LOCUS       DAOMUF010000001        51807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_34_length_51807_cov_3.109658, whole genome shotgun sequence.
ACCESSION   DAOMUF010000001 DAOMUF010000000
VERSION     DAOMUF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 51807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51807
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34_length_51807_cov_3.109658"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLN77_00005"
     CDS             complement(<1..393)
                     /locus_tag="PLN77_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42694.1"
                     /translation="MRERDENAYIRFFALWFAAGMTLGVLLLLTASHTGMKEGNSVRL
                     FQAEAFFGRKISAKACFSYLLKVRLAPAFAGLLFGLTAIGAYFATAAVWAYGFLCGVF
                     LSAAYLQNGMAGLLFFFLALFPQIFLYLA"
     gene            complement(608..3190)
                     /locus_tag="PLN77_00010"
     CDS             complement(608..3190)
                     /locus_tag="PLN77_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRM42695.1"
                     /translation="MKAKVRTFNGTTSPEVTQRETDHRKLAREAAAEGFVLLENKDHF
                     LPLAKGSKVGLYGAGAIRTIKGGTGSGDVNERDSVNIFQGMKNAGYDVTSSEWLEDYD
                     KLYVQARLDWKNEIFTKLNGDDTKFFDAYSATPFFMPSGNPIDEEKAAADGADTAFFV
                     LARIAGENKDRFDTEGDYFISKEEKAILAQVSRCYKNVVLVINTGGLMELAFVEEFDN
                     IRSILQYVQAGQEGGNAFADVVSGDVTPSGKMTDTWAKDYYDYPGAEVYSYKSGDLMK
                     EKYEEGIFVGYRYFDTFEVPVRYSFGYGMSYTDFEIRTDDIKVSGRGMMNPKVSVTVT
                     VTNTGDTYAGKEVVQIYASCPQGRLVKEFRRLAGFGKTKLLAPKESQTMTITFPLYQL
                     TSYEEESASWILEPGMYGIWIGNDLNTSVLSGALELDEKAVMTACENICPLKEKLNEI
                     VPDAEKVQAREAAWQKEVKEKRMSVIELKASEIPTEKVDYQPVPEELPGKAGKIVESL
                     SVDQLALLATGDPGRAQGNALGSAGISVPGAAAETSPCASEEPWNVTTIALVDGPAGL
                     RLKREYQVDNGEIVASDFLAALEGGFFSKPQEKRGTTYYQFCTAIPVGTLLAQSWDVE
                     LIKRVGEMIGHEMELFNVTLWLAPGMNIHRNPLCGRNFEYYSEDPLLAGMMAAAMTLG
                     VQKVPGCGTTIKHFACNNQEDNRMGSDSILSERALREIYLKGFEIAVKDAQPMSIMTS
                     YNLINGVHAANCYDTCTKAARDEWGFAGAIMTDWTTTNVQIQGECTAAGCMRAGNDMV
                     MPGLPEDHENIKKELADGTLTMAELKRCIYNTVNIVLQSNMYEGAVSYLDQFDDLDTY
                     LVVK"
     gene            3378..>3624
                     /locus_tag="PLN77_00015"
     CDS             3378..>3624
                     /locus_tag="PLN77_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM42696.1"
                     /translation="MESCFYETIHETDLLHVRFELQDAPAFVFPAHWHEYVEILYLIR
                     GQFSAIVQATEYQLNEGDLIIINSGDLHMTRSNTCTYL"
     assembly_gap    3625..3634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3635..4199
                     /locus_tag="PLN77_00020"
     CDS             <3635..4199
                     /locus_tag="PLN77_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM42697.1"
                     /translation="QMRQYLPDFDTMRFDTIIPCSEQPAPLRALLSEMNEIRQNPSDS
                     YQLLFTSRLYEFLYHLCRSYSHQIPSASLTGSQRDLQRVTHVMNWVKVHYPEPLSLET
                     AADSLALSKEYFCRLFKKYTGQTFLEYLNDVRTMHLYEDLQNSDETITVLMEKNGLSN
                     YKIFMRTFKKLYGSTPQKMRSNRIRHV"
     gene            complement(4312..5877)
                     /gene="rny"
                     /locus_tag="PLN77_00025"
     CDS             complement(4312..5877)
                     /gene="rny"
                     /locus_tag="PLN77_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRM42698.1"
                     /translation="MGTGITILLVFIAVVVTLLIAVPVTRKIAVDNKIKQDAEKIGTA
                     EEKARNIIDEALKTAETKKREALLEAKEESLRTKNEVEKETRERRAELQKYEKRVLSK
                     EESVDKKADVLERRENEIAAKEASVRKKEKEVDGLHEKGVQELERISGFTSEEAKEYL
                     LKSVEDEVKIDTAKLYKELESRAKEEAGRKAKEYVVTAIQKCAVDHVSESTISVVQLP
                     SDEMKGRIIGREGRNIRTLETMTGVDLIIDDTPEAVVLSSFDPIRREVARIALEKLIV
                     DGRIHPARIEEMVEKARKEVEQNIREEGEAAALEVGVHGLHPELIRLLGRMKFRSSYG
                     QNALKHSIEVAQLSGLLAGEVGCDVRMAKRAGLLHDIGKSIDHEVEGSHIQIGADLCK
                     KYKESQIVINTVESHHGDVEPQSLIACIVQAADAISAARPGARRETLETYTNRLKQLE
                     DITNSFKGVEKSFAIQAGREVRIMVVPEQVSDSEMVLLARDISKQIEAELEYPGQIKV
                     NVIRESRVTDYAK"
     gene            complement(6026..6652)
                     /locus_tag="PLN77_00030"
     CDS             complement(6026..6652)
                     /locus_tag="PLN77_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRM42699.1"
                     /translation="MFVTEIRPLNKKKSRILFDDGEDLVLYNGEIRASRIKEQEELPD
                     EVYEKLAGEVLTKRAKHRAMYLLLGQMRTKKELEDKLRKDGYPERAIEEAVSYVESFH
                     YIDDRAYVEQYLAGSGAKKGKKAVLYALAQKGVDRELVESVLEEQEDARDEYELAKSA
                     AFAKLGTPHRLDEKEYRRAAGFLTRKGFGSSEIRRALEEYQDSADEEF"
     gene            complement(6653..7720)
                     /gene="recA"
                     /locus_tag="PLN77_00035"
     CDS             complement(6653..7720)
                     /gene="recA"
                     /locus_tag="PLN77_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRM42700.1"
                     /translation="MGNNNEDKLRALDAALGQIEKQYGKGAVMKLGDSAANMNVETIP
                     TGSLSLDIALGLGGVPKGRIIEVYGPESSGKTTVALHMVAEVQKRGGIAGFIDAEHAL
                     DPAYAKNIGVDIENLYISQPDNGEQALEITETMVRSGAVDIIIVDSVAALVPKAEIDG
                     DMGDSHVGLQARLMSQALRKLTAAISKSNCIVIFINQLREKVGVMFGNPETTTGGRAL
                     KFYSSIRLDVRRIEALKQGGEMVGNRTRIKVVKNKVAPPFREAEFDIMFGKGISKEGD
                     VLDLAASVGIVQKSGAWFAYGGDKIGQGRENAKAYLREHPEIMDEIEHKVRVAYGLIK
                     EEAPEGTEETAAVPETEGDDE"
     gene            complement(7994..9436)
                     /gene="glgA"
                     /locus_tag="PLN77_00040"
     CDS             complement(7994..9436)
                     /gene="glgA"
                     /locus_tag="PLN77_00040"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HRM42701.1"
                     /translation="MKNILFVASECVPFIKTGGLADVVGSLPKYFPKEYFDVRVVIPK
                     YAGIKEQFKNQMQYVTNFYMDLNWRQQYVGIFEMEYEGIKFYFIDNEEHFGGSTPYAG
                     MPWDLEKFAYFSKAALSILPTIGFRPDLIHCHDWQTGLVPVYLHERFQADEFYRGIKT
                     VMTIHNLKFQGIWDRKTIQSITGLSDYYFTPDKLEFKHDASYLKGGLVYADAITTVSN
                     TYAQEIQTPFYGEGLDGLMCARANDLRGIVNGLDYNEWNPETDAQIAKNFNVKNFRKE
                     KVKNKLALQEELGLEVNPKTMMIGIVSRLTDQKGFDLISYMMDEMCQDAVQFVILGTG
                     EERYENMFRHFAWKYDKKVSANIYYSDAMSHKIYASCDAFLMPSLFEPCGLSQLMSLR
                     YGTLPIVRATGGLKDTVEPYNEYEGTGTGFSFTNYNAHEMMGTVRYAERIYYDKKREW
                     NKMIDRAMAQDFSWANSAKQYEEMYNWLIG"
     gene            complement(9629..10429)
                     /gene="spo0A"
                     /locus_tag="PLN77_00045"
     CDS             complement(9629..10429)
                     /gene="spo0A"
                     /locus_tag="PLN77_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HRM42702.1"
                     /translation="MEKINIAIADDNERMQEMLGNVIKQDDTLELIGQTGNGNDIYSI
                     IRDKEPDVVLLDIFMPKMDGLTVMEKVNEDKNLKKRPAFIVVSAVGEERITEDAFRLG
                     AYYYVLKPFDNETLLNRIKATKGMTAVRRYHPRNFTNPGKEPICPPENSLEIDVTNMI
                     HEIGVPAHIKGYQYLRDAILLSVEDTEMLNSITKILYPTIAKRHQTTSSRVERAIRHA
                     IEVAWSRGKMDTIDELFGYTVNNRKGKPTNSEFIALIADKIRLELKNQ"
     gene            complement(10636..11853)
                     /gene="spoIVB"
                     /locus_tag="PLN77_00050"
     CDS             complement(10636..11853)
                     /gene="spoIVB"
                     /locus_tag="PLN77_00050"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HRM42703.1"
                     /translation="MTRKQKYRRVLLALAALFLILAVLLGYDAMRESLPDTMYVDENS
                     ESPFQPLTKHPLLTISEDTKEAEKTEFGETGETVVECSAAGIPLKKIRVIQEERPKVA
                     VCGAPVGIYMETDGVMVIRTAEIRSSDGTVSRPSENILREGDYITKVNGQSIRSKQEL
                     IDAVKVSNGSPVCLSLLRDGETIEVQVTPQKSQDGTCRLGAWVRDNMQGIGTLTFVEP
                     DGTFAALGHGISDVDTGKMLQLNGGELYLTQILSVIPGKAGVPGELQGVIHYEEKNRI
                     GSVAENTIYGIHGVLDREKSSALPLTVTEIAYRQEIETGPATVRMCVDNTVEEYEAEI
                     TEIDLGSENTNKNFVLEITDQRLLQKTGIVQGNSGSPVLQNGRLIGAITHVFVNHPEK
                     GYGVFAENMLARN"
     gene            complement(12019..13710)
                     /gene="recN"
                     /locus_tag="PLN77_00055"
     CDS             complement(12019..13710)
                     /gene="recN"
                     /locus_tag="PLN77_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRM42704.1"
                     /translation="MLTNLHVKNLALIDEAEVEFGPGLNILTGETGAGKSILIGSINA
                     ALGKKISREMIRTGETSALVELVFETENPHVLELLKETDLGAEEGQVIISRKITGSRS
                     VCRINGEACNISQVKVLASLLLDIHGQHEHQSLLYPDRQIAILDAFAGTEAATAKGEV
                     RALYQEWKNVRKELSAYELDEEARKREAAFLTFEINEIDQAELKEGEDEALETAYRKM
                     GHAKKIAESLQTVYAITGYGAENSAGEQVGRAIRELQQAAVYDDALSGPSQTLSDIDG
                     LLNDFNREISAYLSELTFSEEEYYETEKRLDEINRLKAKYGKTMEEITAYREEQQKKL
                     EKLENFESCRAALQEQLQKTEQKLDAAAHNLSKIRRNYAKRLETQIIEGLRDLNFLHV
                     AFEISFEKTKSYTENGTDAVEFLISTNPGEAIRPLAKVVSGGELSRIMLAIKTILADR
                     DETETLIFDEIDTGISGRTAQKVSEKMAQIGQRHQVICITHLPQIAAMADSHFEIEKN
                     VEENETVTSIHPLSEEDSVRELARMLGGAKITDSVLANASEMKELAQVQKSARLK"
     gene            complement(13775..14238)
                     /locus_tag="PLN77_00060"
                     /pseudo
     CDS             complement(13775..14238)
                     /locus_tag="PLN77_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948754.1"
                     /note="regulates arginine biosynthesis when complexed with
                     arginine by binding at site that overlap the promotors of
                     the arginine biosynthesis genes; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
     gene            complement(14268..15125)
                     /locus_tag="PLN77_00065"
     CDS             complement(14268..15125)
                     /locus_tag="PLN77_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRM42705.1"
                     /translation="MERFYLITNRAKDPDLTVTDQIRQYLEERGKTCLLCDNSEKGEK
                     YHYTDPAKVPSDIDGILVLGGDGTLLQAAGDVVDLQIPLLGINLGTLGYLAEIDRDTL
                     YPALDHLMADEYTIEHRMMLCGSIYRDGKLIAEDAALNDITITREGNLRVVRFDNYVN
                     GEFLNSYSADGIIIATPTGSTGYSLSAGGPIISPSASLMLMTPLAPHTLNTRSIVFPA
                     EDVIEVELGPSRDGGIEQGMAYFDGDTRVPMKTGDRIVIKKARRDTLIVKISNISFLE
                     TLRKKMANI"
     gene            complement(15145..15954)
                     /locus_tag="PLN77_00070"
     CDS             complement(15145..15954)
                     /locus_tag="PLN77_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRM42706.1"
                     /translation="MKKRLDVLLVERGLATSREKAKAMIMAGEVLVDNEREDKAGSMF
                     PEEVEIVLKGKPLPYVSRGGLKLEKAMKNFNLTLDGKVCMDVGASTGGFTDCMLQNGA
                     VKVYSVDVGHGQLDWKLRNDPRVVCMERTNIRYVVTEDIEERPSFVSIDVSFISLTKV
                     LLPVRNLMEENGEIAALIKPQFEAGREKVGKKGVVRDPAVHKEVIEMVISYAKSISFG
                     VRHLEFSPIKGPEGNIEYLVHLVRLPDGVTEEETNVDVDAVVKSAHDTLDK"
     gene            complement(15951..17828)
                     /gene="dxs"
                     /locus_tag="PLN77_00075"
     CDS             complement(15951..17828)
                     /gene="dxs"
                     /locus_tag="PLN77_00075"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRM42707.1"
                     /translation="MLEKIQKPNDIKKIPADQLPALAEEIREFIIESLSKTGGHLASN
                     LGVVELTIAMHRVFDLPKDKLIWDVGHQSYTHKILTGRKDGFETLRREGGISGFPKRS
                     ESDCDVFDTGHSSTSISAGVGYVRARELKKENYSVVSIIGDGALTGGMAYEALNNAAS
                     LKSNFIIVLNDNEMSITENVGGMSSYLSGLRTASAYTDFKMDVTKALNRIPGIGPGMV
                     DAMRKTKSSIKQIIIPGMLFEDMGLTYLGPVDGHNIPQLIKTFQEAKRFEGPILVHVL
                     TQKGRGYEPAMRHPARFHGAGPFDVKTGLPVGKSNPTYTDVFSTVMRKMGDRRKDVAA
                     VTAAMMTGVGLKRFSNMFPDRCFDVGIAEEHAVTFAAALSLGGITPVVAIYSSFLQRA
                     YDQIMHDVCMQNLHVVFAIDRAGLVGYDGETHHGIFDLSYLGSMPNMTILAPKNLWEL
                     SDMIKFAVDYDGPIAVRYPRGEAYTGLKEFRAPICLGKSEVIHEGSRVALLAVGSMVK
                     MAEEVQKQLKERMDMDAALVNARFVKPIDEELLRSFADTYELVVTLEENVKDGGFGER
                     VLAFAEEEDLPFGVEIIALPDRFIPHGSVSYQMKQVGFTPEDICGRIEEYYRKRGQEE
                     R"
     gene            complement(17840..18760)
                     /locus_tag="PLN77_00080"
     CDS             complement(17840..18760)
                     /locus_tag="PLN77_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRM42708.1"
                     /translation="MRMEHSENFKAVLEEKTREAESIIAAYLPKEEGFAKELAKAMNY
                     SMTAGGKRLRPLLLQESFRLFGGTGEAVKPFMAAMEMIHTHSLIHDDLPAIDNDDYRR
                     GRKTTHVVFGEAVGVLAGDALLNYAYETAWKAFPLEEHTDRVVRAFGILCQKTGISGM
                     LGGQSVDVMNEKAGHFAIDREMLDYIYENKTSALLEAAMMAGAALAGANDDDLDALCR
                     IASKVGIAFQIRDDILDVTSTMEELGKPVFSDEKNEKTTYVTMFGTDGAEQEVKRLTK
                     EAVDELKNLSCPGDKIFLEELFESLTSRKK"
     gene            complement(18732..18977)
                     /gene="xseB"
                     /locus_tag="PLN77_00085"
     CDS             complement(18732..18977)
                     /gene="xseB"
                     /locus_tag="PLN77_00085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRM42709.1"
                     /translation="MGNMEAEERKEEGTPSGETLEAAFGKLDQMLETLESSDVSLEDS
                     FRLYKEGMELLNECSQKIDRVEKKMLQMNEDGTFREF"
     gene            complement(19000..20226)
                     /gene="xseA"
                     /locus_tag="PLN77_00090"
     CDS             complement(19000..20226)
                     /gene="xseA"
                     /locus_tag="PLN77_00090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRM42710.1"
                     /translation="MRNKNQNVYTVGQVNSYIKNIFDQDYMLRRIYVSGEVSNCKYHP
                     SGHIYFSLKDAEGTISCVMFAGSRRGLAFPMRDGDNVIVGGSISVYERDGKYQIYAKE
                     ITKEGAGLLYEKYLALKTELEEMGMFAPEYKQPIPAYVKKIGVVTAPTGAAVQDIKNI
                     ALRRNPFVQIILYPALVQGEGAADSIVEGIRRLDVLNLDVLIVGRGGGSIEDLWAFNE
                     EKVARAVFSCHTPVISAVGHETDTTIIDFVADLRAPTPSAAAELAVADVRAVLNTLEN
                     YKSRMNHAMANQTQRARSRLLQYETKWKYLGPENKIRDQRARHAELERRYTDAMEEKL
                     KEARHRLALYIQAMKGLSPLDKLNQGYSYVEKEGKAITSVQDVKEGDLLEICVSDGKI
                     TARAEKTSAVSWMEQS"
     gene            complement(20216..20653)
                     /gene="nusB"
                     /locus_tag="PLN77_00095"
     CDS             complement(20216..20653)
                     /gene="nusB"
                     /locus_tag="PLN77_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRM42711.1"
                     /translation="MNRSKLRENIFKLLYTAEFQEKEEMPQQMSLYFTYSDDNDEDEE
                     VTVSEKDQTYMEEKYHHVTEHIPEIDEMLNRTSEGWKTTRMGKVDLAILRLAVYEMKF
                     DEDVPVGVAINEAVELAKRFGRDQSPSFINGILGKLAREEDEK"
     gene            complement(20781..21173)
                     /locus_tag="PLN77_00100"
     CDS             complement(20781..21173)
                     /locus_tag="PLN77_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRM42712.1"
                     /translation="MAEAKIQKRNTYTIYDDEGIGTVQIADEVVAIIAGLAATEVDGV
                     ASMAGNITNELVSKLGMKNLSKGVKVDVLDGVVCVDLALNLKYGYEIPATCKNVQEKV
                     KTAIENMTGLEVSDVNVAIAGVNMEQTK"
     gene            complement(21244..22116)
                     /gene="sdaAA"
                     /locus_tag="PLN77_00105"
     CDS             complement(21244..22116)
                     /gene="sdaAA"
                     /locus_tag="PLN77_00105"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRM42713.1"
                     /translation="MSYNSLEEIVRECQEKDLPFDEVILLDDMNERNVSREESIETMR
                     GMWEAMKGAERNYDGSLQSRSGLVGGAGKTMRAYVESGNTLCGPFVGKVMASALAMGE
                     SNACMKRIVAAPTAGACGVLPAVLVNYQKEKGTADEQIVRALYTAAGIGQVVAARAYI
                     AGASGGCQAEIGTASAMAAGALTALGGGTPSQITHAAAMALKNLLGLVCDPVGGLVEV
                     PCVKRNVIGSVNALSAADMALAGIISRIPPDQVINAMREVGDQMHPSLRETGQGGLAN
                     TPAAREWCAAQEEE"
     gene            complement(22106..22789)
                     /gene="sdaAB"
                     /locus_tag="PLN77_00110"
     CDS             complement(22106..22789)
                     /gene="sdaAB"
                     /locus_tag="PLN77_00110"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HRM42714.1"
                     /translation="MNLFDILGPVMVGPSSSHTAGAVRIGWMTQKLLGEQPVEAKILM
                     YGSFAATGKGHGTDRGLVAGLLGMRPDDERIPDSFEIARKMGLSYSFGIANLTSAHPN
                     SVVLEVKGKSGRTLEVQACSLGGGRIMINRLDGLDVNCSCEIPTLIVHNLDQPGHVAE
                     VTSMLAHKSVNIANMSLYRDKRGGSAVMVVEMDQPLPKESLEWLEHLEGIVKVTYINV
                     TEGEEDDVL"
     gene            complement(22828..24420)
                     /locus_tag="PLN77_00115"
     CDS             complement(22828..24420)
                     /locus_tag="PLN77_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRM42715.1"
                     /translation="MPDYTKEIERRRTFAIISHPDAGKTTLTEKFLLYGGAINLAGSV
                     KGKATARHAVSDWMEIEKERGISVTSSVLQFNYDGYCINILDTPGHQDFSEDTYRTLM
                     AADSAVMVIDASKGVEAQTRKLFKVCVMRHIPIFTFINKMDRDANDTFELLDEIEKEL
                     GIATCPINWPIGSGKKFRGVYDRDKKKVLTFSDTMKGTKEGIEEEIDIDDPHLLDVVD
                     EDQKEQLMDEIELLDGASAEFDQELVSKGELSPVFFGSALTNFGVETFLQHFLTMTMP
                     PLPRTADCGVIDPVKEDFSAFVFKIQANMNKAHRDRIAFMRICSGKFDAGMEVFHVQG
                     GKKMRLSQPQQMMASDRHIVDEAYAGDIIGVFDPGIFSIGDTICMPGKKFAYEGIPTF
                     APEHFARVRQLDTMKRKQFVKGISQIAQEGAIQIFQEYKGGMEEIIVGVVGVLQFDVL
                     KYRLENEYNVDIRLENLPYEHIRWIANKEEINLDRIQGTSDMKKVMDLKGNPLLLFVN
                     PWSVGMVQDRNKDLVLTEFSKN"
     gene            complement(24528..25046)
                     /locus_tag="PLN77_00120"
     CDS             complement(24528..25046)
                     /locus_tag="PLN77_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HRM42716.1"
                     /translation="MIESSFTLYKLIILYMLQNVNFPLSNTQISDFILEKEYTNYFHL
                     QEALSELQDTKLIEIEKVRNTSYYHMTEEGSKTLSFFENEISPEIREEIDRYMKEHSY
                     ELRKKASVVADYYKTAGDDYEVRCQARERKTTIFEIVVTVPSEEAAKKICSNWENRSQ
                     KIYASIMNELSR"
     gene            complement(25043..25990)
                     /locus_tag="PLN77_00125"
     CDS             complement(25043..25990)
                     /locus_tag="PLN77_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706535.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRM42717.1"
                     /translation="MTDWLGKPYYSLDAYLKKTFGEKVYKLSLDGGMTCPNRDGTCGD
                     RGCIFCSAGGSGDFAGDRNKSIPEQIREQKSLLQQKRPVHKFIAYFQAYTNTYAPVEY
                     LEKIFREAISDEEVVVLSIGTRPDCLGEEVLSLLEELNRIKPVWVELGLQTMHEKTAQ
                     YIRRGYPLSCFEQAVNELHRRGIPVIVHTILGLPGEGQEEVFATMDYLNGLSISGIKL
                     QLLHVLKNTDLAADYAAGKFEVLEQDAYLTLVIDCLRRLRPDLVIHRVTGDGPGDLLI
                     APTWSQAKRTVLNELHHRMKEEHAYQGQLLDEEKGGNTP"
     gene            complement(26005..27327)
                     /locus_tag="PLN77_00130"
     CDS             complement(26005..27327)
                     /locus_tag="PLN77_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRM42718.1"
                     /translation="MDLFDYMKETTMEKESPLASRMRPTTLDEVVGQEHIIGPGKLLY
                     RAIKADKLGSVIFYGPPGTGKTTLARVIANTTSAEFTQLNATTAGKKDMEEVVKEAQQ
                     RIGMYGKKTILFVDEIHRFNKGQQDYLLPFVEDGTLVLIGATTENPYFEVNGALISRS
                     IIFELKPLEKEDIEKLLERAVTDPVKGLGTYNVVLDADAKEFLADIAGGDARAALNAI
                     ELGVLSTERQADGKIHIDLETASECIQKRVVRYDKTGDQHYDTISAFIKSMRGSDPDA
                     AVFYLAKMLYAGEDIKFIARRIMICAAEDVGLADPNALNVAVSASLAVERVGMPEAQI
                     ILAEAATYVACAPKSNSSCEAVFAAMKTVGSVRTSVPPHLQDAHYKGSAKLGHGEGYQ
                     YAHDYPNHYVEQQYLPDEILGSRFLHLGTNGYEQKIRENFKKIGKDIE"
     gene            complement(27441..28115)
                     /locus_tag="PLN77_00135"
     CDS             complement(27441..28115)
                     /locus_tag="PLN77_00135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM42719.1"
                     /translation="MRHVPLPLLAVIMAAILFAELARCEREGAKETAAEAEKSVPRRA
                     ALTFDDGPNACYTPEVLEILKENEIPATFFLQGQCLAGNEEIVRRMHAEGHLIGNHTF
                     HHVQLTKVSEEEAREEVVKTSNAIYEITGEYPVYIRPPFGEWREGLDLAVTMIPVLWD
                     VDSRDWESQNTASICSEVLPNVKDGSIILMHDGYRATVEALRRILKELKEEGYTFVTV
                     RELIEE"
     gene            complement(28232..>28851)
                     /locus_tag="PLN77_00140"
     CDS             complement(28232..>28851)
                     /locus_tag="PLN77_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42720.1"
                     /translation="FILFGICMSYIITRFLLWKKERILQVFGGIGLVLLLWTFGYFGT
                     SVYSWFGNVFDLSEYRGLDATAYLENVFSEDAGAIRWLDETIKGQPVVLEANGDSYSD
                     YERVSAMTGLPTVLGWYVHEWLWRGDPADLNVRAEDVKQMYTSTDTNEVLRLLEQYHV
                     TYIFVGSKEKEKYGDALNESLLQSIGDIVYQDTASGTYILQVQDT"
     assembly_gap    28852..28861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28862..30637)
                     /locus_tag="PLN77_00145"
     CDS             complement(<28862..30637)
                     /locus_tag="PLN77_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HRM42721.1"
                     /translation="MSKKTTVSGILLVLLVLATTPLLGTDNVSFLKWWLMTLVLGIGF
                     YPAAAALFPRFHDRGWMFSKVLGIVVSGFAVFALGSFGLVPFTAPVCLITVGVLILAS
                     GIFGCFKVRVHAPEIDFLMMEEVLFFAFFLLWTYLAGFHPEAHGTEKFMDYGFMKAMM
                     RSTAVPAEDLWYSGSGINYYYGGQFYAVFLTKITFTDVKQTYHLTRTMVASFAFVLPF
                     SITYHLAESRACHRIRKEGGNKSQIAPVLGGLLSGGAVSLAGNMHYVIYGCIRQWLGL
                     NESAYWFPSSTRYIGYDPLVENDRTIHEFPSYSFVLGDLHAHVVNVMFVLLVLGLLYS
                     YVKNTCRDPEKEWKWSLKDVLLQPQIIAAGFLIGVFHWSNYWDFVIYFVVIAGFSLYG
                     ALYRYHARAKETIGTVLLQAAEAFAIGTIVALPFTMKFETMVSGVGIAKHHSMLYQLA
                     ILWGLPTVLVVLFIAAVLLAWRKNCHLPGMERQGQIVLADGKTQEEVEEQAVALILGE
                     KKPEPGEKETAEKPKKVSAFCNFWREIAVSDMVIGILGLCAIGLIIIPELVYVRDIYE
                     ESYARSNTMFKLTYQAYILFGMTMGY"
     gene            complement(30640..32271)
                     /locus_tag="PLN77_00150"
     CDS             complement(30640..32271)
                     /locus_tag="PLN77_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM42722.1"
                     /translation="MIAANNVTLRVGKKALFEDVNIKFTEGNCYGMIGANGAGKSTFL
                     KILSGQLEPTNGDVTITPGQRLSFLEQDHFKYDSYPVLDTVIMGNARLYEIMKEKEAL
                     YAKEDFTDEDGIKASELEGEFAEMNGWEAETDAEQLLNGLGIEPELHYAQMADLTGSQ
                     KVKVLLARALFGNPDILLLDEPTNHLDLDAIEWLEEFLINFENTVIVVSHDRYFLNKV
                     CTQIADIDYGKIQLYAGNYDFWFESSQLLVKQMKEANKKKEEKIKELQDFISRFSANA
                     SKSKQATSRKKALEKIQLDDIKPSSRKYPYIDFRPNREIGNDVLQVEGLTKTVDGVKI
                     LDNLSFTLGREDKVAFVGSNEQAITMLFKILTGEEEPDSGYYKWGVTTTQAYFPKDNT
                     AEFDCDLTIADWLTQYSEIKDVTYVRGFLGRMLFAGEDGVKRVRVLSGGEKVRCLLSK
                     MMISGANVLVLDEPTNHLDMESITALNNGLIKFPGVLLFTCHDHQFVQTTANRIMEIL
                     PNGALIDKMTTYDEYLASDEMARKRHVYTMTEEDN"
     gene            complement(32393..34462)
                     /locus_tag="PLN77_00155"
     CDS             complement(32393..34462)
                     /locus_tag="PLN77_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRM42723.1"
                     /translation="MQKFYQRLKENQKERARCAFLVLYFGVLAVLLFLARPLLDTTAA
                     DREWSIHFLFPCLLACIILTTVVSFCRFAAKPDQKPKPRYVGWKQPILMLANAAYLFA
                     TLEFVTNSQFREMKWYYALLNIGVIFVLSIFVSLFLNSIRRAMIFMNIFYFCMSLVFY
                     YVYLFRGEAFQLIDLYSIATAADVVGGYKFEITGEIVTSFITMMLVVRLWLQSREYRF
                     ARKTRNKILLRVAAAALTLGTYLAYMNLNWNAEFGVISDLWNPAKTYRQYGTTVGFTA
                     VAKYMRLTPPDGYSKDEVTAIADTSEKETKTEDLRKDNADSVTPVNIIAIMNESWFDY
                     RSVGDPQTSESYMPFLDSLTENIIKGHTLTCTKGGGTAKTEYEFLTGNSCKRFPGMVP
                     YVSYFTHSQYSLVTTLKDQGYQAIAMHPNKATNWKRSTAYRFLDFDDFISIDQFSDTA
                     KRYRGMISDQANYEKIVETYENKEPGSPFFLFDVTMQNHSGYTNRYFQADINCNGYDS
                     DEADQYFSLLKLSDEAISYLIRYFQQVDEPTMIIMFGDHSPKLPDAFETWIAGDAYQN
                     LSVEDQEKFYGTPFFIWTNYSMKSESNVWISTNYLSSYALSLTGLELTPYNEYLLDLR
                     EKMPALNHLGFLASDGTWYRWNDSDAPEEDLEYERQYECLQYNELIDKKDRDDSFFTI
                     SGEKDEE"
     gene            complement(34494..35267)
                     /locus_tag="PLN77_00160"
     CDS             complement(34494..35267)
                     /locus_tag="PLN77_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HRM42724.1"
                     /translation="MKESIHTIPLTDAFKAEDECPFCYLEREAEQHAISFALGSGASY
                     MEDDVRAETDAMGFCRHHYKMMYDYGNRLGSGLILSTHLKKLNQELAAQMDLFAPGKS
                     SVFKRMQKTSLDKQGRETAIGQWIDEKTHSCYVCDHFKANYNRYLDTFFDLYKKDEEF
                     ARLFREGKGFCLPHFADLVETAEKKLNDKQKAEFYPVLFKIMKENYQRLQEEVTWFTD
                     KFDYRNKDKDWGNSKDSIQRCMQKLGGGYPADEPFTEGL"
     gene            35505..35738
                     /locus_tag="PLN77_00165"
     CDS             35505..35738
                     /locus_tag="PLN77_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM42725.1"
                     /translation="MGERHTEEMRLLGLTIAFYRRARGLTQSELAEAVQISRTHMSNI
                     EAPNSHTSLSLNKLMDIAEALEIPLKTLFDFKR"
     gene            complement(35828..36760)
                     /gene="metA"
                     /locus_tag="PLN77_00170"
     CDS             complement(35828..36760)
                     /gene="metA"
                     /locus_tag="PLN77_00170"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HRM42726.1"
                     /translation="MPVKVQADLPVKEILESENIFVMDETRAVHQDIRPLKIMILNLM
                     PLKEDTELQLLRSLSNTSLQVDVTFLMVASHEAKNTSTSHLNTFYVKFENVRKNYYDG
                     MIITGAPVEQMEFEEVDYWDELTKIMDWTNTHVTSTMFLCWGAQASLYHFYGLKKRML
                     PEKKFGLFWHKVNNRKIPLVRGFDDEFLAPHSRHTEVPIDDIRACKDVTILAESDEAG
                     FYLGMAEEGRKIFVMGHPEYDRMTLDGEYHRDKDKGLPIEIPKNYYKNDDPNTKPVLL
                     WRSHANNLYTNWLNYYVYQVTPYNLDGTPDFSGK"
     gene            complement(36912..38867)
                     /gene="ligA"
                     /locus_tag="PLN77_00175"
     CDS             complement(36912..38867)
                     /gene="ligA"
                     /locus_tag="PLN77_00175"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRM42727.1"
                     /translation="MNETERMKELVKKLNEAAKAYYQEDREIMSNLEYDTLYDELQVL
                     EEKTGVTLAGSPTTKVGYEAMDELPKEAHESPMLSLDKTKDVEVLRSFIGDHKTLLSW
                     KMDGLTVVLTYRGGTLAKAVTRGNGVIGEVVTNNAKVFENIPLKIPYQGELVLRGEAI
                     IRYSDFEKINEEIEDVDAKYKNPRNLCSGSVRQLNNEITAKRHVHFMAFALVSAPEQD
                     FENSRIRQMEWLKEQGFEVVEYKVVTRDNLDEAMQYFSTKIETNDFPSDGLVALYDDI
                     AYGESLGTTAKFPRNAFAFKWADEQKETTLLEIEWSPSRTGLINPVAVFEPVELEGTS
                     VSRASVHNISIMRSLDLGVGDKILVYKANMIIPQIAENLTRSGVRDIPETCPACGGAT
                     RIQMMNNVETLYCTNPECPAKFMKAFTLFVSRDAMNIDGMSEATLEKFVGHGFIREFA
                     DIFRLDRYRDEIVEMDGFGEKSYQNLIDSIERAKNTTLPRLIFGLGILNIGLANARMI
                     CKAFDFDLDRIRNASAEDFAQIDGVGEVIAKSIVDYFADEENKERLERLLSYLTIEKP
                     QASQEEQKLAGLTFVITGSVEHFPNRNALKARIEELGGKATGSVTGKTSYLINNDTTS
                     SSSKNRKARELGVPVISEEEFLKMTEE"
     gene            complement(38864..39565)
                     /locus_tag="PLN77_00180"
     CDS             complement(38864..39565)
                     /locus_tag="PLN77_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM42728.1"
                     /translation="MRLNKYLSDAGVCSRREADRLTEAGEITVNGKRAETGMQVESGD
                     VVVVKGKTVVPEEKKVYLAFNKPRGIVCTAEKREKNNVIDYLSYPVRIYPVGRLDKDS
                     EGLLLMTNDGAIVNGIMRARNRHEKEYQVEVNKEITPEFLKKMASGVPILDTVTRPCR
                     IRKTGERSFTIILTQGLNRQIRRMCEALGYRVTKLKRVRIMNIHLKDLKTGEYRPLTE
                     KELAELKKQIGEEKR"
     gene            complement(39641..40519)
                     /locus_tag="PLN77_00185"
     CDS             complement(39641..40519)
                     /locus_tag="PLN77_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42729.1"
                     /translation="MAKPMTTKDKIREWISDNLRYMLLIGAILLIVAAVLLIVHLVSP
                     KNDGKSSGGTVTSSSTGNNVPDKKTQVKADSKADSEDSTGISSDVSDSANDSSADASS
                     TDTASAPDAEAEPTSEPEAADDTSNTENTDNTAVSFAADNVPEVSTVLEQYYTALGAR
                     DINGLFAVTDNLTAEEQAQIEAESDVESYGDVKAYTISGPSDGTYITFVSSRCKYLGI
                     NQTLPMLSEYYLYTKEDGSLKIMDDTDSDAAVTEAMKAALENEEVKNLIEQVQNDYQN
                     ALDADASLRAYVESIQ"
     gene            complement(40664..40736)
                     /locus_tag="PLN77_00190"
     tRNA            complement(40664..40736)
                     /locus_tag="PLN77_00190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40701..40703),aa:Arg,seq:ccg)
     gene            complement(40864..41775)
                     /locus_tag="PLN77_00195"
     CDS             complement(40864..41775)
                     /locus_tag="PLN77_00195"
                     /inference="COORDINATES: protein motif:HMM:NF039828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5688 family protein"
                     /protein_id="HRM42730.1"
                     /translation="MDYQMFKEQLAALLKRKAGDDFEVKVESVQKLNGIEKEAFVISR
                     KKDWIAPTIYVELLYECCLQGVPLSQIAEKVLEQYRKVEAEARRPEKAAFFTNWKNAA
                     PQIYCELIHAEKNRTLLSEVPHRKFLDLAVVYYYQMRGNRVPDATILIHETHRELWGI
                     SAEELDARAWENTLRDLPVRTDSLMVYLKEEYGVTFPLFELGPLAEQFYLVSNRRGKL
                     GAICMCYPGVLESLSQRFEASLYVIPSSIHECMVIPDYDVFPEECLSDMVRDVNEKTV
                     KPQEILSDHVYYYHRESGELTLCEEET"
     gene            complement(42044..42601)
                     /gene="efp"
                     /locus_tag="PLN77_00200"
     CDS             complement(42044..42601)
                     /gene="efp"
                     /locus_tag="PLN77_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRM42731.1"
                     /translation="MISAGDFRNGVTLEIDGQVVQILEFQHVKPGKGAAFVRTKLKNV
                     INGGVVERTFRPTEKFPQARIERVDMQYLYSDGDLFYFMNVENYEQIALNEETIGDAL
                     KFVKENEMVKVCSYNGNVFAIEPPLFVELEITETEPGFKGDTAQGASKPATVETGATV
                     SVPLFVNQGDKIKIDTRTGEYLSRV"
     gene            complement(42686..43210)
                     /locus_tag="PLN77_00205"
     CDS             complement(42686..43210)
                     /locus_tag="PLN77_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HRM42732.1"
                     /translation="MMRMFYPDRREKSAYTIDYADLYRKGYRGVIFDIDNTLVPHGAP
                     ATKEAAAFFASLRETGFSTCLLSNNQKPRVAPFADAVGSIYIENAHKPSVKNYQEACR
                     RMGIKEEQAVFVGDQLFTDVYGAKRTGLYNILTEPINPKEEIQIVLKRYLEHIVLFFY
                     ERDLARGRITKKNS"
     gene            complement(43285..44172)
                     /locus_tag="PLN77_00210"
     CDS             complement(43285..44172)
                     /locus_tag="PLN77_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HRM42733.1"
                     /translation="MNETKNYSITKSGAVILEGGANRGVFTAGALDFLMEQEYYIPHV
                     IGVSAGACNALDYVSRQIGRTRDCMIVTDEKNRYVNKNIKTIVEKKALLDMDMVFERY
                     PYEIFPFDFDTYFASPQTCELVVTNCETGRAEYLDDRENKERLLAIGRASSSMPIACP
                     MVEIDGNEYVDGGVADSIPIIRSLKTGHRKNVIILTRNFGYRKKEGTRGWELYVAAFR
                     KYPNLVRALVNRAKHYNQVLECIEKWEEEGSVFVIRPEIPAVSRLEKDEEKLREFYQH
                     GYDQMKANFEALQEFLKEE"
     gene            complement(44277..45635)
                     /locus_tag="PLN77_00215"
     CDS             complement(44277..45635)
                     /locus_tag="PLN77_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRM42734.1"
                     /translation="MAFSMKVTPEIEELTNICLDNSKMDVSLYGQYDVKRGLRDINGV
                     GVLAGLTQISNVVSSDIIDGVKTPCDGRLYYRGINVEKLTQGFLSENRMGFEEVAYLL
                     LFGNLPNEEQLNHFKTILKQQQSLPKNFVRDVVMKAPTKDMMNTLSRSVLTLYAYDNH
                     ADDTSLPNVLRQCINLISVFPMLSVYGYQAYNHYIKGKSLYIHNPSKKLSTAENILLM
                     LRPDQKYTPLEAKILDLALVLHMEHGGGNNSTFTTHVVSSSGTDTYSAIAAALGSLKG
                     PKHGGANIKVVRMFDDMKSHVKDWTDEEEVSNYLRKLLHKEAFDQKGLIYGMGHAVYS
                     ISDPRARIFKSFVEQLAKEKHREKDYRLYAMVEELAPKIIGEERHIYKGVSANVDFYS
                     GFVYSMLDLPLELYTPMFACARIVGWSAHRMEELINTDKIIRPAYKSVKDEVDYVPLE
                     NR"
     gene            complement(45766..46401)
                     /locus_tag="PLN77_00220"
     CDS             complement(45766..46401)
                     /locus_tag="PLN77_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HRM42735.1"
                     /translation="MEVSNYIKMRDQMQERFLTYDQKEIEGKYGFAFDDDYFYVPYLF
                     EEWRVNRHTGVVEGSKDHFLHVHQGSFNETLTIFDLLCCGKKEIVLAGTYARTNSLKG
                     TIKGRTIASGVHDSRAARYDGHVEQLKKACVSLSGKPYSPGDAAFVFHVFPFLPLVFQ
                     FWESDEEFPPVLKFMWDENVLDYLRFETLYYVMGDFLDALDQAFDACKTEA"
     gene            complement(46416..46868)
                     /gene="nrdR"
                     /locus_tag="PLN77_00225"
     CDS             complement(46416..46868)
                     /gene="nrdR"
                     /locus_tag="PLN77_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRM42736.1"
                     /translation="MRCPFCNQDNTRVVDSRPIEDSNAIRRRRMCDVCGRRFTTYEKV
                     ETIPLIVIKKDMNREQYDRSKIETGILRACHKRPVSAEKINEMVNKVETELFNGEERE
                     IPSSVIGEKVMHYLKDLDQVAYVRFASIYREFKDVNTFMDELKKMLDK"
     gene            47011..47262
                     /locus_tag="PLN77_00230"
     CDS             47011..47262
                     /locus_tag="PLN77_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HRM42737.1"
                     /translation="MRVVDLRQKEVINICTCKTLGCPIDVEFNPKTGHLTGLVLPGPV
                     KFCGFFCHGEDFVIPWDRICQIGNDIILVEIPEKEKKQP"
     gene            complement(47263..47568)
                     /locus_tag="PLN77_00235"
     CDS             complement(47263..47568)
                     /locus_tag="PLN77_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRM42738.1"
                     /translation="MNETEKRDRRDEKEEIRYHVIFYGQVQGVGFRYRAYYAAYQLGL
                     TGWVRNCWDETVEMEVQGDEETITEMIKRIRTSSYIEITDAKWTKIPLESEFGFHIR"
     gene            complement(47596..48762)
                     /locus_tag="PLN77_00240"
     CDS             complement(47596..48762)
                     /locus_tag="PLN77_00240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM42739.1"
                     /translation="MTEYRLAKPEEWEDCIELANYVFSTAHRPHDFEQLIPRVYQAGP
                     EMARIHRVAVAENGRLRAEIAVLPQQMAAGGKLLRAGYVGSVSVHPKARGEGHMKRLL
                     GDWITDLEGTCDLLALDGQRQRYAYFGFTPGSEKYTFYLDVANVRHTWKNGELSAYTF
                     APLFPENGEADEEAAAFAKKLNEEKPLYVVREDVKACLKTFGEQAIAIWKNGERIGYL
                     ALCGGNQVVEAEVLEEQDFVPALAAYLKENALDSLFISIPVYETGKAAALSEVCESFT
                     KERCGSAMYRIFQFADVIEAMLTMKAETMGISDGTWFAVLEGQPLTVTVKDGTVTVTR
                     EAHPGADVLNREQAQELLLSPLASKGSKVPSEIWKNIPSDWFPLPLYCATADEF"
     assembly_gap    48924..48933
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(49017..51557)
                     /locus_tag="PLN77_00245"
     CDS             complement(49017..51557)
                     /locus_tag="PLN77_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM42740.1"
                     /translation="MEQYNVTGMSCAACQTRVEKAVSKVPGVTSCAVSLLTNSMGVEG
                     TASPAEIIAAVENAGYGASKKGEKGSTSQSASTAEYEEMLKDKETPVMKRRLVSSVFL
                     LIPLMYFSMGHMMWGWPLPKFFEGNHIAMGLVQLLLTIAVMVINQKFFISGFKSLWHK
                     APNMDTLVALGSTASFVYSVYALFAMTGAQVQGDMDAVMSYMHEFYFESAAMILTLIT
                     VGKMLESISKGKTTDALKSLMKLAPKTAVLLRDGKEVEVGIDQVKKGDHFIVRPGENI
                     PVDGVVLEGTSAVNESALTGESIPVDKEAGDRVSAATLNQSGFLTCEATRVGEDTTLS
                     QIIQMVSDAAATKAPIAKVADKVSGIFVPAVICIALATIVIWLFAGESVGFALARGIS
                     VLVISCPCALGLATPVAIMVGNGMGAKHGTMFKTAVSLEKTGKTQIVALDKTGTITSG
                     EPQVTDLVPADGVSEEELLKGAFALEQKSEHPLARAILALAEEKGLAREEVSDFSALP
                     GNGLVAKRNGKELCGGNRALITKKAVLSKQMEEQAAKLSEEGKTPLFFSEDGKMLGMI
                     AVADVIKEDSPRAVKELQNMGIRVVMLTGDNERTARAIGAQAGVDEVIAGVLPDGKEA
                     VISRLKEKGKVAMVGDGINDAPALTSADIGIAIGAGTDIAIDAADVVLMKSRLSDVPA
                     AIRLSRATLRNIHENLFWAFIYNIIGIPLAAGVWIPLFGWKLNPMFGAAAMSLSSFCV
                     VTNALRLNLFRMHDASKDKRIKNEIPKEDLNMTTEQGMVKTMTIEGMMCGHCEARVKK
                     TLEAIEGVASAEVSHEKGTAVVTLTAPVSDDVLKTAVEAQDYTVKGIQ"
BASE COUNT        11144 a        14281 c        11827 g        14525 t
ORIGIN      
        1 ggcaagatag aggaaaatct gcggaaaaag agccagaaaa aagaataaaa gacctgccat
       61 tccgttctgc agataggcag ccgagagaaa gacgccacac agaaaaccat atgcccaaac
      121 ggcagcagtg gcaaaatacg caccgattgc cgtcaatcca aagagaagtc ccgcaaatgc
      181 cggtgctaac cgtaccttta gaagatagga aaagcaggcc tttgcggaaa tttttcttcc
      241 aaaaaacgcc tccgcctgaa agaggcggac gctatttccc tccttcattc ccgtgtggga
      301 tgccgtaagc agcagcagta caccgagggt cataccggcc gcaaaccaga gggcaaaaaa
      361 acgaatatat gcattctcat cccgttctct catttgcttt ttgcgcctcc ttattttctg
      421 ccttactgtc aatgtattca cggttcatag acaatatacg taaaagaagt gcctgctccg
      481 caaaccccca tctgcgaatg gcacttcttt cctcttttca gggtccccca atgtctcacg
      541 caccgaaatg tccgttcatt tcaaaccctg ctttttgtat atccccataa gatatttttc
      601 acttctttta tttcacgaca agataggtat ccagatcatc gaactgatca aggtagctga
      661 ctgcgccctc gtacatattg gactgcagta cgatatttac cgtgttatag atgcagcgtt
      721 tcagttctgc catcgtcaga gttccatctg caagttcttt tttgatgttc tcgtgatcct
      781 ccggcagacc cggcattacc atgtcatttc cggctctcat gcagcccgct gcggtacact
      841 cgccctggat ctgtacgttg gtggttgtcc agtcggtcat aatggcacct gcaaatcccc
      901 actcatctct cgctgctttc gtgcaggtat cgtagcagtt tgcagcgtgt acgccgttga
      961 tcagattata agatgtcata atggacatcg gctgtgcatc tttgactgca atctcgaagc
     1021 ctttcaggta aatctcacgc agtgcacgct cggacaggat ggaatcggag cccatacggt
     1081 tatcctcctg attgttgcat gcgaagtgtt tgatcgtggt tccgcagccc ggtaccttct
     1141 gtacaccgag tgtcatggca gccgccatca taccggcaag cagcggatcc tcggaatagt
     1201 attcaaagtt tcttccgcag agcggatttc tgtggatgtt cataccaggt gccagccaca
     1261 gtgttacgtt gaacagttcc atctcatggc cgatcatttc gccgactctc ttgatcagtt
     1321 ctacatccca gctctgtgcc agaagtgttc caaccggaat tgcggtacag aactgataat
     1381 aggttgttcc gcgtttttcc tgcggtttgc tgaagaaacc gccttccagg gcagccagga
     1441 aatcgctcgc tacgatctct ccgttgtcta cctgatattc tctcttcaga cgaagacctg
     1501 caggtccgtc gaccagagca atcgtggtta cattccacgg ctcctccgaa gcgcacgggg
     1561 atgtctccgc tgctgcaccc ggaacagaaa ttcctgcgga gccaagcgcg ttgccctgtg
     1621 ctcttcccgg atcaccggtt gcaaggagtg ccagctgatc cacagaaagg gattccacaa
     1681 tctttcctgc ttttccagga agctcttccg gtaccggctg gtaatctact ttctcagttg
     1741 ggatctcaga tgctttgagc tcgatcacgc tcattctctt ttcttttact tccttctgcc
     1801 atgcagcctc tcttgcctga actttttctg catcaggaac aatctcattc aatttctctt
     1861 tcagcgggca gatattttca cacgctgtca tgactgcttt ctcgtccagc tccagcgcgc
     1921 cggagagaac agatgtattc agatcatttc caatccagat accatacatg cccggctcca
     1981 gaatccagga tgcgctttcc tcctcatagg aagtcagctg atacagcggg aaggtgatgg
     2041 tcatggtctg ggattccttc ggagccagca gttttgtttt tccaaagcct gccagacggc
     2101 ggaactcttt caccagtctg ccctgcgggc aggaagcgta gatctgtaca acttcttttc
     2161 ctgcataagt atcgccggtg ttggtgacgg taacagtcac gcttactttc ggattcatca
     2221 taccgcggcc gcttactttg atgtcatcgg tcctgatctc gaaatcggtg taggacatac
     2281 cgtaaccgaa ggagtaacgg actggtactt caaaggtatc gaagtaacgg tatccaacga
     2341 agataccctc ttcgtatttt tctttcatga ggtcgccgct cttgtagctg taaacctctg
     2401 cacccggata atcatagtaa tcttttgccc aggtatctgt catttttccg gatggggtta
     2461 catcgccgct tacaacatca gcaaatgcat ttccgccttc ctggcctgcc tgtacgtact
     2521 gtaaaataga gcggatattg tcaaattctt ctacgaatgc cagctccatc aggccgccgg
     2581 tgttgataac aagaacaacg tttttatagc atctggaaac ctgtgccaga atagctttct
     2641 cttctttgga gatgaagtag tcaccctccg tatcaaaacg gtctttgttc tctcctgcga
     2701 tacgtgccag aacgaagaat gcggtgtctg caccgtcggc tgctgctttc tcttcatcga
     2761 ttgggtttcc ggatggcata aagaacgggg ttgcagagta agcatcaaag aattttgtgt
     2821 cgtctccgtt caattttgtg aaaatctcat ttttccagtc cagtcttgcc tgtacgtaga
     2881 gtttatcgta atcctccagc cattcactgc ttgttacgtc gtagcctgca tttttcatgc
     2941 cctggaaaat attgacgctg tcacgctcat tgacatcgcc ggaaccggtg cctcctttga
     3001 tggtgcggat cgcacctgct ccgtaaagac ctaccttgct gccctttgcc agcggcagga
     3061 aatgatcttt gttttccaga agaacgaatc cctctgctgc tgcctcgcgg gcaagtttgc
     3121 ggtgatctgt ttcacgctgg gttacttccg gagatgtagt tccgttaaag gttcttacct
     3181 ttgctttcat gtctttgttc ctcccaaact ttgtttcgtg ctaatgtctc cattatcttc
     3241 cctttttcgt gtaacttcta gttcctttct tgtcctttct ctggttgata ttgatatttc
     3301 gcccaatttc tggtacaata ttcttatctt ttttgattat aattgacgta tatttctatc
     3361 acatcctgga ggtacttatg gaatcctgct tttacgaaac aatacacgaa accgatctgc
     3421 tgcacgtccg ttttgagctg caggatgcgc ccgcttttgt ttttccggcg cactggcacg
     3481 aatatgtgga aattctctat ctgatcagag gacagttttc cgcgattgtc caggcaacgg
     3541 agtatcagtt aaacgagggt gatctgatta tcatcaactc cggcgacctc catatgacgc
     3601 gctccaacac ctgtacatat cttcnnnnnn nnnnccagat gcgccaatat ctgccggact
     3661 ttgatacgat gcgtttcgac acgatcattc cctgctcgga acagccggct ccgctccgcg
     3721 cactgctctc ggagatgaac gagatccggc agaatccttc cgattcatac cagcttctct
     3781 ttacctcacg actgtacgaa tttctgtacc atctctgccg gtcgtattcc caccagatcc
     3841 cttcggcatc cctcacgggt tcacagcgcg atctgcagcg cgtgacacac gttatgaact
     3901 gggtgaaagt gcattacccg gaaccgttaa gcctcgaaac agccgcggat tccctggcgc
     3961 tctcgaagga gtatttctgc cggctcttta aaaagtatac cggacagacc tttctcgaat
     4021 atctgaacga tgtgcgcacg atgcacctgt acgaagattt acagaattcc gacgaaacga
     4081 tcacggtact gatggagaaa aacgggctgt cgaattacaa aatttttatg cggaccttca
     4141 aaaagctgta tggctctaca cctcagaaaa tgcgctcgaa ccggatacgg cacgtataaa
     4201 ttcttttgaa atttactgtt cttatacaca ctgaaaaggc tgtggaacat gattgctcat
     4261 gtccacagcc tctttttgat ttcgttatta aattgtttcg tagaaagtct tttatttcgc
     4321 gtaatccgtt acacggctct cgcggataac attgaccttg atctgtcccg ggtattccag
     4381 ttcagcttcg atctgtttcg agatatccct tgcgagcagt accatttcag aatcggatac
     4441 ctgctcagga acaaccatga tgcggacttc tctaccagcc tgaatagcaa aagatttttc
     4501 gacacccttg aacgaattcg tgatgtcttc caactgtttt aatctgttgg tatatgtttc
     4561 gagtgtttct cttcttgcac ccggtcttgc tgcggaaatt gcatccgctg cctgaacgat
     4621 acatgcaatc agagactggg gctctacatc cccgtgatgt gattctactg tgttgatcac
     4681 aatctgagat tctttgtact ttttgcagag atctgcaccg atctgaatgt gagatccttc
     4741 tacctcatgg tcgatggatt taccgatatc atgaagcagt ccggcacgtt ttgccatgcg
     4801 gacatcgcat cctacctctc ctgcgaggag accggaaagc tgtgctacct cgatcgaatg
     4861 ctttaatgca ttctgtccgt agctggaacg gaatttcatt cttccaagga gacggatcag
     4921 ctccggatga agtccgtgta caccgacttc cagtgcggct gcctcgcctt cctcgcgaat
     4981 attctgttct acttctttgc gggccttttc taccatctct tcgatgcggg ccggatggat
     5041 tcttccgtct acgatcagtt tttccagagc aattcttgcc acctcacggc ggatcggatc
     5101 gaatgaggaa agaacgactg cttccggtgt atcgtcgatg atcaggtcaa cgcctgtcat
     5161 ggtttcgagt gtacggatgt ttcgtccttc acggccgatg atgcggcctt tcatttcgtc
     5221 gctcggaagc tgtaccacgg agatggtact ctcggatacg tgatcgacag cacatttctg
     5281 aatagctgtt accacatact cttttgcctt acggcctgct tcctcttttg ctctgctctc
     5341 cagctctttg tagagctttg ctgtgtcgat ttttacttcg tcttccacag attttaacag
     5401 atactctttt gcttcctcgg aggtaaaacc ggaaattctt tccagttcct gtactccttt
     5461 ttcgtgtaga ccgtctactt ccttttcttt ctttctgacg cttgcctctt ttgctgcaat
     5521 ttcgttttca cgacgctcca atacgtccgc ttttttatct acagattctt ctttcgataa
     5581 aacgcgcttc tcatatttct gcagttccgc tctgcgctct cgggtctctt tttctacttc
     5641 attcttcgta cgaagagact cctccttcgc ctccagcagg gcttcccgtt tcttagtttc
     5701 ggcagttttc agtgcttcat ctatgatgtt tctcgccttt tcttccgctg tgccaatttt
     5761 ctcagcatcc tgcttgattt tattgtccac agcaattttc ctggtaacag gaaccgcaat
     5821 aagcagcgtg actactacag caatgaatac cagcagaatt gtaatacctg tacccattac
     5881 cggagcacct ccttatttag ttttcattgc acatttacaa cactacaatt ttatactgtt
     5941 ttcacaaatt tgtcaatgtt ttaaaaatat tcatgtaatt tattgcacat aatttcgcgg
     6001 catgtggcgg aatcgccggg aatattcaaa attcttcgtc agcggaatct tggtattctt
     6061 ccagtgcccg acggatttcg gaactgccga atcctttccg cgtcagaaaa ccggcggcac
     6121 ggcggtactc tttttcatcc agccggtgcg gcgtgccgag ctttgcgaag gcggcacttt
     6181 ttgcaagttc atactcatcg cgggcatcct cctgctcttc caggacggat tccaccagtt
     6241 cccggtccac gcctttctgt gccagcgcat agaggactgc ctttttccct tttttcgcac
     6301 cgctgcccgc aagatactgc tccacgtatg cgcggtcgtc gatatagtga aagctctcca
     6361 cgtacgagac agcctcttca atggctctct cgggatagcc gtcttttcgt aacttatctt
     6421 ccagctcttt tttcgtccgc atctgtccga gcagcagata catcgcgcgg tgtttggccc
     6481 ttttagtcag cacttcccct gccagctttt cgtaaacctc atcgggaagc tcctcctgct
     6541 ctttgatgcg ggaagcgcgg atttccccgt tataaagaac cagatcctct ccgtcatcga
     6601 agaggatccg tgatttcttt ttattcagag gacgaatctc tgtcacaaac atttattcgt
     6661 cgtctccttc ggtttccgga actgctgctg tttcttctgt gccttccggc gcttcttcct
     6721 tgatcaggcc gtaagccacg cgcactttgt gctcgatctc atccatgatt tccggatgct
     6781 cgcgcaggta tgctttcgca ttttcgcgtc cctggccgat cttgtcgccg ccgtatgcga
     6841 accatgcgcc gctcttctgt acgatgccga cggaagctgc cagatccaga acgtctcctt
     6901 ctttggagat tccttttccg aacatgatat caaattctgc ctcgcggaac ggcggagcta
     6961 ctttgttttt cacgactttg attctggtac ggtttccgac catctctccg ccctgcttta
     7021 aggcttcgat tcttctgaca tcgagacgga tggaagaata gaatttcagt gcacggccgc
     7081 cggttgtggt ctccggattt ccgaacatga caccgacttt ttcacgcagc tggttgataa
     7141 agataacaat acaattggat ttgctgatcg ctgcagtcag tttccggagt gcctgagaca
     7201 tcagacgcgc ctgcagtccg acgtgggaat ctcccatgtc gccgtcgatc tcggcttttg
     7261 gaacgagggc tgcaacagag tccacgatga tgatgtctac cgcaccggaa cgaaccatcg
     7321 tctcggtgat ctcaagtgcc tgctcgccgt tgtccggctg tgagatatag aggttttcaa
     7381 tatcaacacc gatgtttttg gcataggccg gatcgagcgc atgctctgca tcgatgaagc
     7441 cggcgattcc gccgcgtttc tgtacttcgg ctaccatgtg gagcgctacg gttgttttac
     7501 cggaagactc cggtccatat acctcgatga ttcttccctt cggcacaccg ccaagaccga
     7561 gtgcgatatc caggcttaaa gagccggtcg ggatggtttc cacgttcatg tttgctgcgg
     7621 aatctccgag cttcatgaca gctccttttc cgtactgttt ttcaatctgt ccaagtgcgg
     7681 catcgagtgc acgcagttta tcttcgttat tatttcccat actgaatatt ctccttacca
     7741 tgcgaacgtt cgttcgcttc tgtatacata ctattacaaa tctgctttgt tgtcaactgt
     7801 ttttcttgtg tcttttttca gcccccaaat cctctgcacc ggagttgctt cgaatatatc
     7861 cttatgcaca agtgtagcat attccactct cctgccgcaa cccaaaaata ctgcaaaaaa
     7921 atcccgaaac ggcttttctt tcctcttctg ccatttcggg atttttttct tatttttcag
     7981 aatgcagacc agcttatccg atcagccagt tgtacatttc ctcgtactgt tttgcggaat
     8041 tcgcccagga gaaatcctgt gccattgcgc ggtcgatcat cttgttccac tcgcgttttt
     8101 tgtcgtaata gatgcgctct gcgtaccgca cggtacccat catctcatgc gcattgtagt
     8161 ttgtgaagct gaatccagtt cctgttccct cgtactcatt gtacggctct acggtgtctt
     8221 tgaggccgcc ggtcgcgcgg acgatcggaa gggttccgta gcggaggctc atgagctggg
     8281 agagtccgca tggctcaaac agggacggca tcaagaaggc atcgcaggat gcatagatct
     8341 tgtgtgacat tgcatcggaa tagtagatgt ttgccgaaac cttcttgtcg tatttccatg
     8401 cgaagtggcg gaacatgttc tcgtagcgct cctcgccggt tccaaggatc acaaactgta
     8461 cggcatcctg gcacatctca tccatcatat agctgatgag gtcgaagccc ttctgatcgg
     8521 tcagccggga tacgataccg atcatcatcg tcttcggatt gacctcgagt cctaactcct
     8581 cctgcagtgc cagtttattt ttcactttct ctttacggaa gttctttaca ttgaaattct
     8641 ttgcaatctg agcgtctgtc tccgggttcc actcattgta gtcgagtccg tttacaatac
     8701 cgcgcagatc gtttgctctt gcacacatca gaccgtcgag tccttcgcca tagaacgggg
     8761 tctgaatctc ctgcgcgtat gtattgctga cggtggtgat cgcatctgcg taaaccagac
     8821 cgcccttcaa gtagctggca tcgtgtttga actccagctt atccggtgtg aagtagtagt
     8881 cggaaaggcc ggtaatgctc tgaatcgtct tgcggtccca gatgccctgg aatttcaggt
     8941 tgtggatcgt catgacggtt ttgattcccc gatagaattc atctgcctgg aaacgctcgt
     9001 gcaggtaaac cggaacgaga ccggtctgcc agtcgtggca gtggatgagg tccgggcgga
     9061 agccgatggt cggtaaaatg gaaagcgctg ctttgctgaa gtaagcaaat ttttccagat
     9121 cccacggcat gcccgcatat ggagtgcttc ctccgaaatg ctcctcgttg tcgataaagt
     9181 agaatttgat gccttcatat tccatctcga aaatgccgac atactgctgt ctccagttca
     9241 gatccatgta gaagtttgtc acatactgca tctgattttt aaactgttct ttgattcctg
     9301 cgtattttgg aatcaccacg cgcacatcaa aatactcttt cgggaagtac ttcggcagag
     9361 aacctactac atccgccaga ccgcctgttt tgatgaacgg tacacactct gaagccacga
     9421 acaaaatatt tttcatgaaa agaatcctcc tgtaatttca tgctgattat cgcatctttg
     9481 tttttctgta attccttcct ttttgtcacg atcctatgaa acgcgaaagg ataatcaacg
     9541 tcctgtctgt attataccgt actttgtcca gaaaaaaaag tctaaacttc tgtaatttct
     9601 ccgtttgttc ggattttttt gttacttttt actgattttt cagttcaaga cgtattttat
     9661 ctgcaattaa tgcaataaat tcagaatttg tcggtttacc cttgcggttg ttcacggtat
     9721 agccgaacag ctcgtcgatt gtgtccattt tgccgcggct ccaggccact tcaatcgcat
     9781 ggcggatggc acgctcgaca cgacttgacg ttgtctggtg gcgtttcgcg atggtcggat
     9841 agagaatctt ggtaatggaa ttgagcattt ccgtgtcctc tacggaaagc agaatcgcat
     9901 cgcgcaggta ctggtatcct ttgatatggg caggaacgcc gatctcgtgg atcatatttg
     9961 tcacatcgat ttccagactg ttttccggcg gacagatcgg ctcttttccg gggtttgtaa
    10021 aatttcttgg atggtatctt cttactgctg tcattccttt tgttgctttg atccggttca
    10081 gaagagtttc gttgtcgaac ggttttaaga cgtaatagta agcgccgagc cggaacgcat
    10141 cttcggtgat cctctcctct cctacggcgg agacaacgat aaaggcaggt ctttttttca
    10201 gatttttatc ctcattgact ttctccatta cagtcagacc atccatcttc ggcatgaaaa
    10261 tgtcaagcag tacgacgtcc ggttctttgt cgcggatgat actgtagata tcatttccgt
    10321 ttccggtctg cccgatcagt tcaagcgtgt catcctgttt tatgacgttc ccaagcattt
    10381 cctgcatgcg ttcgttatca tccgcgatgg caatgttgat cttttccata aagctctcct
    10441 ttgcaaaaac tcatagtctt tccggaattt cactgcatcc gttttcttct gaactttttc
    10501 caatcacaca gacattataa ttttattttt tcgcattttc aatatatttc ccgtaaattt
    10561 ttgaaaattc gatcgacatt ttccgtaaaa attcggaagt ttgtaacaat ttcaaccaaa
    10621 ttaacgcata ttttttcagt tccgtgcaag catgttttcc gcaaagacac cgtagccttt
    10681 ttctggatgg tttacgaaaa catgggtaat ggcgccgatc agccttccgt tctggagcac
    10741 cggcgagccg gaatttccct gtacgatgcc ggttttctgc agcagccgtt gatcggtaat
    10801 ttcgagcaca aaatttttgt tcgtattctc gcttccaaga tcgatttccg taatctccgc
    10861 ctcatactct tctaccgtat tgtccacgca catccgcacg gtggccgggc ccgtctcgat
    10921 ctcctgccgg taggcaattt ccgtcacggt aagcggcagt gcactgcttt tttccctgtc
    10981 cagaacgccg tggataccgt agatggtatt ctccgccact gacccgatac ggttcttttc
    11041 ttcgtaatgg atcacgccct gcagttcacc cggtacaccc gcttttcccg ggatcacaga
    11101 cagaatctgt gtcagataca gttctccgcc gttcaattgg agcatttttc cggtatcgac
    11161 atcactgatt ccatggccga gcgctgcaaa ggttccgtcc ggttccacga aagtcagtgt
    11221 tccgattccc tgcatattgt cgcggaccca ggccccgagg cggcaggtgc cgtcctggct
    11281 tttctgcggt gtcacctgca cctcgatcgt ttccccgtcc cgaagcaggg aaagacacac
    11341 cggagaaccg tttgatactt ttactgcatc aatcagctcc tgcttgctgc ggatgctctg
    11401 tccgtttact tttgtgatat agtcgccttc ccgcagaata ttttctgacg gacggctgac
    11461 ggttccgtcg gacgaacgga tttcggctgt acggatcacc atcacaccgt cggtctccat
    11521 atagataccg accggcgcgc cgcagacggc aactttcggc cgttcttcct ggatcacacg
    11581 gatctttttc agcgggatcc cggcggcgga acattctaca accgtttcac ccgtttctcc
    11641 gaattcggtt ttttctgctt ccttcgtatc ttccgaaatc gtcagaagcg gatgtttcgt
    11701 gagcggctga aacgggcttt cgctgttctc atccacatac atcgtatccg gcagggattc
    11761 gcgcatggca tcgtagccaa gaagaacggc gaggatcaga aacagcgccg ccagcgcaag
    11821 caggactctt cggtattttt gttttctcgt catttgcatt cccatccttt ccgccgcagg
    11881 gcagaactgc ggcatttctt ttgttttcgc ggcggtcgtc aagttctcgt gcaggcacgg
    11941 actttgactc gccgcccgcg aaaaagaggg aaaccacgct ttgtttcgtg atttccctct
    12001 tagtatgcgg acttctcttt atttgagtct tgcagatttt tgtacctgtg ccaattcctt
    12061 catctcgctg gcatttgcca gaacagaatc cgtaattttc gcgccgccga gcattcttgc
    12121 cagttctctg acagaatctt cctcggataa tggatggatc gatgtgacgg tctcgttttc
    12181 ttcgacgttt ttttcgattt caaaatgaga atctgccatc gctgcgatct gcggcagatg
    12241 ggtgatgcag atgacctgat ggcgctgccc gatctgtgcc attttttcgg aaactttctg
    12301 cgccgtcctt ccgctgattc cggtgtcgat ttcatcgaaa atcagggttt ctgtctcgtc
    12361 gcggtcggca agaatcgtct tgattgcaag catgattcgc gacagttcac cgccggaaac
    12421 aaccttcgcc agcggacgga tcgcttcgcc cgggtttgtg gagattaaga actccacagc
    12481 atcggttccg ttttcggtgt agctttttgt cttttcaaat gaaatttcaa atgcgacatg
    12541 caggaaattc aggtcgcgca gaccttcgat aatctgtgtt tcgagccgtt ttgcatagtt
    12601 tctgcggatt ttggacagat tatgtgcggc cgcatccagt ttctgctccg ttttctgtaa
    12661 ctgctcctgc agtgcggcac ggcagctctc aaagttttcc agtttttcca gttttttctg
    12721 ctgttcttcg cgatatgcgg tgatctcttc catcgtcttt ccatactttg ctttcaggcg
    12781 gttgatctca tcgagccgct tttccgtctc gtaatactct tcttcggaaa aggtcagttc
    12841 ggaaagatat gcggaaattt cgcggttgaa atcattcagc agtccgtcaa tatcggaaag
    12901 ggtctgcgac ggaccggaaa gtgcatcgtc gtacaccgcg gcctgctgca gctcccggat
    12961 tgcgcggccg acctgctccc cggcgctgtt ctctgcaccg tagccggtga tcgcatatac
    13021 cgtctgcaga gattccgcga tctttttcgc atggcccatt ttccggtacg cagtttccaa
    13081 cgcttcgtcc tcgccctctt ttagctctgc ctgatcaatc tcgttgattt caaacgtaag
    13141 aaatgctgcc tcccgctttc ttgcctcctc gtcgagttcg tacgccgaga gctcctttcg
    13201 gacattcttc cactcctggt acagcgcccg gacctctcct tttgccgtgg ccgcctcggt
    13261 tcccgcaaac gcgtcaagaa tcgcgatctg ccggtccgga tagagcagcg actggtgctc
    13321 atgctggccg tggatgtcga gtagcaggga cgcaaggact tttacctgcg agatattgca
    13381 tgcctcgccg ttgatgcggc agacgctgcg ggatccggtg atctttctgg aaataatcac
    13441 ctgtccctcc tccgccccga gatccgtctc ttttaacagc tccagcacat gcggattttc
    13501 ggtttcaaag accagttcca ccagcgcgct tgtctctccc gtccggatca tctcgcggga
    13561 tatcttcttt ccaagcgccg cgttgatcga accgatcaag atggattttc cggctccggt
    13621 ttctccggtc agtatattca ggcccggacc gaactccact tccgcttcgt cgatcagggc
    13681 cagatttttt acgtgtaagt ttgtgagcat tcttttcctc cggtgtcaca cctgctgtca
    13741 tctttttgtc tctttttgca gagacgtgcg ttctttagaa ctcttcgccc gtctcctcca
    13801 tgccgtccac catttttttc agtcgttctg ctgccacaag cgccgcatca tcgtctcttg
    13861 ccacgcagag aatcgtatcg tctccgccga tgcagccgac aatttcattc cagttcatct
    13921 catcgaaagc cgctgctgcc gccattgcca tgccggatac ggaacatttt tggcaagatc
    13981 aacagaaata aacgcgtctt ttaacacgcg cgcatagcgg ctcccaacct cggacggtgc
    14041 gttctgcaga accgcatagc ggcttccgcc gttctctttt ggtacttttg tgagcttcag
    14101 ctggcggata tccctcgaaa tcgtcgcctg cgttacggca aagccgctct cctccagctt
    14161 ctttgccagc tcctcctgtg tttcaatgtc atattggtta attaatttga taatctgtgc
    14221 atgtctcgcg attttcattt caagcatcct tccccagtaa ctatatctta aatgtttgcc
    14281 attttctttc ttaatgtctc cagaaaactg atgttgctga tcttcacaat cagagtatcc
    14341 cgtctggcct ttttgatcac gatccggtcg ccggttttca tcggaacgcg ggtgtcgccg
    14401 tcaaaatatg ccatgccctg ttcaatgccg ccgtctctcg aaggcccgag ctccacctcg
    14461 atcacatcct ctgccggaaa gacaatgctt ctcgtattca gggtgtgcgg ggcaagcggt
    14521 gtcatcagca tcaggctggc tgacggtgag atgatcgggc cgcccgcgga gaggctgtag
    14581 ccggtcgaac cggtcggcgt tgcgatgatg atgccatcgg cgctgtagga atttaaaaac
    14641 tccccgttga cgtaattgtc gaaccggacg acacgcaggt ttccctcacg ggtgatcgta
    14701 atgtcgttga gtgccgcgtc ctccgcaatc agctttccgt cccggtaaat cgatccacag
    14761 agcatcatgc ggtgctcaat cgtatactca tctgccatca gatgatccag cgccggataa
    14821 agcgtgtcac ggtcgatttc cgcaagatag ccgagcgttc ccagattgat gccgagcagt
    14881 ggaatctgta aatccaccac gtcgcctgct gcctgcagaa gcgtgccgtc accaccgagc
    14941 accagaatgc cgtcgatatc tgacggaacc ttcgccggat ccgtataatg gtatttttcc
    15001 cctttttcgc tgttatcgca gagaagacac gtttttccgc gctcctccag atattgcctg
    15061 atctggtctg tcacggtaag gtccgggtct tttgctctgt ttgtgatcag gtaaaatcgc
    15121 tccatatcat gctccttaac ccttttattt atccagtgta tcgtgtgcgc tctttacaac
    15181 ggcgtctaca tcgacattgg tctcttcctc tgtcacgccg tccggcagcc ggacaaggtg
    15241 aacgagatac tcgatatttc cctccggtcc tttgatcgga gaaaattcca gatggcgcac
    15301 gccgaaggaa atgcttttcg cataggaaat gaccatctca atgacttctt tgtgtacagc
    15361 cggatcacgg acaacacctt ttttgccgac tttttcgcgt cctgcctcaa actgcggctt
    15421 gatcagagct gcaatttcgc cgttctcttc catcagattc cggaccggaa gcagcacctt
    15481 ggtcagggag ataaacgata cgtcgatcga tacgaaggac ggtctctcct cgatatcctc
    15541 cgtcacgacg taacggatgt tcgtgcgctc catacagaca acgcgcggat cgttccgcag
    15601 cttccagtcg agctggccgt ggccgacatc cacggaatat accttgacgg cgccgttctg
    15661 cagcatacag tcggtaaagc cgccggtgga tgctccgaca tccatgcaga cctttccatc
    15721 cagggtaagg ttaaagtttt tcattgcttt ctctaatttt aagccgccgc ggctcacata
    15781 cggcagtggt tttcctttta agacaatctc cacttcttcc gggaacatgg aaccggcttt
    15841 gtcctcccgc tcattgtcca ccagaacttc tcctgccata atcatggctt tcgctttttc
    15901 ccgcgatgtg gcaaggcccc gttctaccag taatacatct aatcgttttt tcatctttct
    15961 tcctgccccc gttttctgta gtattcctca attctgccgc agatatcttc cggcgtaaag
    16021 ccgacctgtt tcatctggta tgatacactt ccgtgcggaa taaagcggtc cggcagtgca
    16081 atgatctcca cgccgaacgg aaggtcttct tcctcggcaa aagcaaggac gcgctctcca
    16141 aagccgccgt cttttacatt ttcctcgagt gttacgacaa gctcatacgt atcggcaaag
    16201 cttcgaagaa gttcttcatc gattggtttg acgaaccgcg cattcacaag cgccgcatcc
    16261 atatccatcc gctcttttaa ctgtttctgc acttcttctg ccatttttac catactgccg
    16321 acggcaagaa gtgcaacacg gcttccctca tggatcactt ctgattttcc gaggcagatg
    16381 ggcgcacgga actcttttaa tcccgtatac gcttcccctc tcgggtagcg caccgcgatc
    16441 gggccgtcgt aatcgaccgc aaatttgatc atatccgaaa gctcccagag gttcttcggt
    16501 gcaagaattg tcatattcgg catcgaaccc agataggaaa ggtcgaaaat gccgtggtgt
    16561 gtctccccgt cgtagcccac aagaccggcg cggtcaatcg caaagacgac atggagattc
    16621 tgcatacaga cgtcatgcat gatctgatcg tacgcccgct gcaggaatga agaataaatc
    16681 gcaacgacag gcgtaatgcc gccgagtgac agtgccgctg caaatgtcac cgcgtgctct
    16741 tctgcgattc ccacatcgaa acagcggtcc gggaacatgt tggagaagcg tttcagtccg
    16801 acaccggtca tcatcgccgc cgtgactgcc gcaacatcct ttcttcgatc tcccattttc
    16861 cgcatgaccg tggaaaagac atccgtgtac gtcggattgg attttccaac cggcaatcct
    16921 gttttcacat cgaacggacc cgcaccgtgg aatcttgccg ggtggcgcat cgccggctca
    16981 tagccgcggc ccttctgtgt caggacatgc accagaatcg gtccttcaaa ccgttttgct
    17041 tcctggaagg tcttgatcag ctgcggaatg ttgtggccgt ccacaggacc taaatacgta
    17101 agtcccatat cctcaaacag catgcccgga atgatgatct gtttgatgct gcttttcgtc
    17161 ttccgcatcg catccaccat gccaggtccg atgcccggaa tgcggttcaa tgcctttgtg
    17221 acgtccatct tgaaatccgt gtacgccgat gccgtccgaa ggccggacag gtacgatgac
    17281 ataccgccga cattctcggt gatcgacatt tcgttgtcat ttaaaacgat aataaaattc
    17341 gatttcaggc tggccgcatt gttcagcgcc tcgtacgcca tgccgccggt cagcgcacca
    17401 tctccgatga tcgaaacaac ggaataattt tcttttttca gttcccgtgc acgcacatag
    17461 ccgacgcctg cggaaatcga ggtcgagctg tggccggtgt cgaatacatc gcagtcactc
    17521 tcgctccgct tcgggaatcc gctgatgccg ccttcccggc gcagcgtttc aaacccgtct
    17581 ttccgcccgg tcagaatctt gtgcgtgtag gactggtgtc ccacatccca gatcagcttg
    17641 tcttttggaa gatcgaagac gcggtgcatc gcaatcgtca gttccacgac accaagattg
    17701 gacgccagat ggccgccggt tttactcaaa gactcgataa taaattcccg gatttcctct
    17761 gcaagcgccg gaagctggtc cgccggaatc ttcttaatat cgtttggttt ctgtattttt
    17821 tctaacatga aatcatccct tacttttttc tggaagtcag agattcaaac agctcttcca
    17881 gaaatatttt atctcccgga caggacagat ttttcagttc gtctacggcc tctttcgtga
    17941 gccgttttac ctcctgctcc gccccgtctg tgccaaacat cgtcacatac gttgtttttt
    18001 cattcttttc atcgctgaaa accggctttc caagctcctc catcgtggat gtgacatcca
    18061 ggatgtcgtc ccggatctgg aacgcgattc cgactttgga ggcaatccgg cagagcgcgt
    18121 caagatcatc gtcgtttgct cctgcaagtg ccgcacctgc catcatggca gcctctaaaa
    18181 gcgccgaagt cttgttctcg taaatgtagt ccagcatctc acggtcgatg gcgaaatggc
    18241 cggctttttc attcatcacg tcaacactct gaccgcccaa catgccggaa attccggttt
    18301 tctgacagag aatgccaaaa gcacgcacta cgcggtcggt gtgctcctcc agtggaaagg
    18361 ctttccaggc tgtctcgtat gcatagttta aaagggcgtc tcctgccagc acgccgacgg
    18421 cctccccgaa gacgacatgg gtcgtttttc tgccgcggcg gtaatcgtcg ttgtcgatcg
    18481 cagggagatc atcgtggatc agggaatggg tgtgaatcat ctccatggct gccataaatg
    18541 gttttactgc ctccccggtt ccgccgaaca gacggaaact ctcctgcaaa agcagcggac
    18601 gaagcctctt tccgcccgct gtcatactgt aattcatggc ttttgccagt tcctttgcaa
    18661 agccttcctc tttcggcagg taagcggcaa tgatactttc cgcttcccgc gttttttctt
    18721 cgagtaccgc tttaaaattc tctgaatgtt ccatcctcat tcatctggag catttttttc
    18781 tcgacgcggt caattttctg actgcattcg ttcaaaagct ccattccctc cttgtataaa
    18841 cgaaaggagt cttccaggct gacatcgctg ctctccagcg tttcgagcat ctgatccagt
    18901 tttccaaatg ctgcttccag tgtctcaccg gaaggggttc cttcttcttt tctctcttct
    18961 gcttccatat ttcccatgtt ttctcgattc tctgcttttt cacgattgtt ccatccagga
    19021 tacggccgat gtcttctctg cgcgcgctgt gatctttccg tctgatacac agatttcgag
    19081 cagatcacct tctttgacat cctgcaccga agtgatggct tttccttctt tttccacgta
    19141 ggaatagccc tggttcagct tgtccagcgg ggacagaccc ttcatcgcct gaatgtaaag
    19201 ggcaaggcgg tggcgcgctt ctttgagctt ttcttccatc gcatctgtat accgacgctc
    19261 cagctcggcg tggcgtgctc tctggtcacg gatcttattt tccggtccga gatacttcca
    19321 ctttgtctcg tactgcagaa ggcggcttct cgctctctgc gtctggtttg ccatcgcatg
    19381 gttcatgcgg cttttataat tttccagtgt gttgagtacc gcgcggacat cagcgactgc
    19441 cagctccgcc gctgcggacg gggtcggggc gcgcagatct gccacgaaat cgatgatcgt
    19501 tgtgtcggtc tcatggccga cggcggagat caccggcgta tggcaggaaa aaacggcacg
    19561 cgctactttt tcctcattga acgcccaaag atcctcgatc gaaccgcctc cgcggcccac
    19621 gatcagcaca tccagattca gtacgtccag acggcgtatg ccctccacga tgctgtccgc
    19681 cgctccctct ccctgcacca gggccgggta gaggatgatc tggacaaagg gattccggcg
    19741 gagtgcaatg tttttgatat cctgcacagc agctcccgtc ggtgctgtga caacgccgat
    19801 tttctttaca tacgcaggaa tcggctgctt gtactccggg gcaaacatgc ccatctcttc
    19861 cagttctgtt tttagggcca gatacttttc gtataaaagg ccggcgccct cttttgtgat
    19921 ctcttttgca tagatctgat actttccgtc ccgctcatag acgctgatgc tgccgccgac
    19981 aatcacgtta tcgccgtccc gcatcgggaa ggcaaggccg cgccgcgaac cggcgaacat
    20041 gacacaggaa atggttcctt ccgcatcttt taaggaaaaa tagatgtggc cggacggatg
    20101 gtatttacag ttgcttactt ctccgctgac gtaaatccgg cggagcatat agtcctggtc
    20161 aaagatgttt ttgatgtatg aattgacctg gcctacggtg taaacattct gatttttatt
    20221 tctcatcttc ttctcttgcc agttttccaa gaataccgtt gataaaggac ggagactggt
    20281 ctctgccgaa gcgttttgcc agctcaacgg cctcgttgat ggctacaccg accggaacgt
    20341 cctcgtcgaa tttcatctcg taaacggcaa gacgcaggat cgccaggtct acttttccca
    20401 tacgggtcgt tttccagccc tcggaagtac ggttcagcat ctcgtcgatt tccgggatgt
    20461 gctcggtcac atgatggtat ttctcttcca tataggtctg atctttttcg cttacggtta
    20521 cttcttcatc ctcgtcgttg tcgtcagagt acgtaaagta aagagacatc tgctgcggca
    20581 tctcctcttt ctcctggaac tctgcggtat agagaagttt aaaaatattt tcgcgaagtt
    20641 tgcttctgtt cattgtctgt tttaattcct tcctgtatag ttaattaact atttttcttc
    20701 catttcatct ggctctgaat agttaacaag aaccgcgggg ttgtaagggg tcgccttatt
    20761 ccctcgcggt tcgttgtttt ttatttcgtc tgttccatat tcacgcctgc aatggctaca
    20821 ttgacatcgg aaacttccag gcctgtcata ttctcgatcg ctgtttttac tttttcctgt
    20881 acgtttttgc aggttgccgg aatctcgtat ccatatttga gattgagggc aagatccacg
    20941 cagacaacgc cgtcgagtac atcaactttg acacccttgg aaagattttt catgccaagc
    21001 ttgctgacta attcgttggt aatatttccg gccatggagg ctaccccgtc cacttccgtt
    21061 gccgcaagtc ctgcgataat agctaccacc tcgtctgcaa tctgcacggt tccgattcct
    21121 tcgtcatcat atatcgtata agtgtttctt ttctgaattt ttgcctctgc catcttaaag
    21181 aacctcctcc ttggctgact ttcctttatt ataccaaaag ttttccgaaa ataaaagttt
    21241 tttttattct tcttcctgtg ccgcgcacca ctcgcgtgct gccggcgtgt tggcaaggcc
    21301 gccctggccg gtttcgcgca gactcggatg catctggtcg ccgacttccc gcatcgcatt
    21361 gatcacctga tccggcggaa tgcggcttat gattcccgca agtgccatgt ccgccgcaga
    21421 aagtgcattc acggaaccga tcacattccg cttgacgcac gggacctcga caagaccgcc
    21481 gaccgggtca cacacgagac cgagcaggtt ctttaatgcc atcgctgccg catgcgtgat
    21541 ctgggacgga gtgccgccgc caagtgcggt cagtgcgcct gccgccatcg cgcttgctgt
    21601 tccgatttcg gcctggcagc cgccggaagc gcccgcaatg taggcccgcg ccgcaacgac
    21661 ctggccgata ccggctgccg tgtagagcgc acgcacgatc tgctcatccg ctgtcccctt
    21721 ctctttctgg taattcacga gcacagccgg aagcacaccg cacgctcctg cggtcggtgc
    21781 tgcaacgatc cgcttcatgc aggcgtttga ctcacccatc gcaagtgcag atgccataac
    21841 ttttccgaca aacggtccgc agagcgtgtt cccgctctcg acatacgcgc gcatcgtctt
    21901 tccggcgccg cccacaagac cgcttctcga ctgcagcgaa ccgtcgtaat tccgttcagc
    21961 gcctttcatg gcttcccaca tgccgcgcat cgtctcaatg gactcttcgc ggcttacatt
    22021 tctctcattc atatcgtcca gaaggatgac ttcatcaaac ggcaggtctt tttcctggca
    22081 ctcccggaca atttcttcca gtgaattata agacatcgtc ctcttctcct tccgttacgt
    22141 tgatgtatgt tacctttaca atgccctcaa gatgctccag ccattccagc gactctttgg
    22201 gaagcggctg atccatctcc acgaccatga cggccgatcc gcctctcttg tcgcggtaga
    22261 gggacatatt cgcaatgttg actgacttat gtgccagcat cgacgtaacc tcggctacgt
    22321 ggcccggctg gtccagattg tggacgatca gggtcggaat ctcgcagctg cagttgacat
    22381 ccagcccgtc gaggcggttg atcatgatac ggccgccgcc aagggagcac gcctgcacct
    22441 cgagcgtccg gccgctcttt ccctttactt ccagaacgac gctgttcgga tgggcactcg
    22501 tcagattggc gatgccgaat gaataggaaa gacccatctt ccgggcaatt tcaaagctgt
    22561 ccggaatccg ctcgtcatcc ggccgcatgc cgagaagacc cgcgacgaga ccgcggtccg
    22621 ttccatggcc ctttcccgtt gcggcaaagg aaccgtacat cagtattttt gcttccaccg
    22681 gctgttcccc gagaagtttc tgcgtcatcc atccaatccg caccgccccc gctgtgtgcg
    22741 aactcgacgg accgaccatc acagggccca gaatatcgaa taagttcatt ccttctcctt
    22801 tttaagcggc atcccgctct tttgttctta gtttttgctg aattcggtca gcacaaggtc
    22861 tttgtttcga tcctgtacca tgccgacact ccacggattg acaaagagaa gcagcggatt
    22921 tcccttgagg tccatgacct tcttcatatc ggatgtaccc tgaatacggt ccaggttgat
    22981 ctcctctttg tttgcgatcc agcggatgtg ctcatacggc aggttctcca gacggatatc
    23041 cacgttgtac tcattctcca gacggtattt taaaacgtca aactgcagga cgccgacaac
    23101 gccgacgatg atctcctcca tgccgccttt gtactcctgg aaaatctgga tcgcaccttc
    23161 ctgtgcaatc tgggaaattc ccttgacgaa ctgttttctc ttcatcgtat cgagctggcg
    23221 cactcttgca aaatgctccg gcgcgaaggt cggaattccc tcgtatgcga attttttgcc
    23281 cggcatacag atcgtatctc cgatggagaa gatgcccggg tcaaatacac cgatgatatc
    23341 gccggcgtat gcttcatcaa caatatgacg gtcactcgcc atcatctgct gcggctggga
    23401 aagacgcatt ttctttcctc cctgcacatg gaagacttcc attccggcgt cgaatttgcc
    23461 ggagcagatg cgcatgaatg cgatacggtc acggtgtgct ttgttcatgt ttgcctggat
    23521 tttaaagaca aacgcagaga aatcctcttt gaccggatcg atcacgccgc agtctgccgt
    23581 acgcggaagc ggcggcatcg tcatcgtgag gaagtgctgc aggaaagtct ccacaccaaa
    23641 gttggtgagc gcagatccga agaataccgg ggagagctct cccttgctga caagctcctg
    23701 gtcaaactct gcacttgcgc cgtcgagcag ctcgatctca tccataagct gttctttctg
    23761 gtcctcatcg accacatcga gaagatgcgg atcgtcgata tcaatttctt cctcgatacc
    23821 ttccttcgtt cccttcattg tatcggagaa ggtaaggact ttctttttgt cacggtcgta
    23881 aacaccgcgg aatttctttc cggaaccgat cggccagttg atcgggcagg ttgcaatgcc
    23941 gagctctttt tcgatctcgt cgagcagctc gaaggtatcg ttcgcatcgc ggtccatctt
    24001 gttgatgaag gtaaagatcg ggatgtggcg catgacacag actttaaaca gctttctggt
    24061 ctgtgcctcg acacccttcg atgcatcgat aaccatgacg gcggaatcgg ctgccatcag
    24121 ggtacggtac gtatcctccg agaagtcctg gtgtcccggg gtatccagaa tgttgatgca
    24181 gtagccgtcg taattgaact gcagaacgga agaggtaacg gaaataccac gctctttctc
    24241 gatctccatc cagtcggata cggcatggcg cgcagtcgct tttcctttta cggaaccggc
    24301 gagattgatc gctcctccgt acagcaggaa tttctcagtc agtgtggttt taccggcatc
    24361 cgggtgggaa atgatggcaa aggtacgccg tctttcaatt tcttttgtat aatcaggcac
    24421 ggttttatct cctttagaat ataaaatctt atttactatg ttttccatgt ttatgctttt
    24481 tagacatact aatttattct attatatgcc accattcctg tcaatcgtta tcttgacagt
    24541 tcattcataa tcgaagcata aatcttctga ctccggtttt cccagttgct gcagatcttt
    24601 ttggccgcct cctcggacgg aaccgtcacg acaatctcaa aaatagtcgt cttccgctct
    24661 cttgcctgac agcggacctc gtagtcatct cccgcggtct tgtagtaatc ggccacaacg
    24721 gacgcttttt tccgcagttc ataggagtgt tccttcatat agcggtcgat ttcctcccgg
    24781 atttccggag agatctcgtt ttcgaagaag gaaagcgtct tgctgccctc ctcagtcatg
    24841 tggtagtacg aggtgttgcg caccttctcg atctcgatca gctttgtatc ctgcagctcg
    24901 cttaacgcct cctggagatg aaagtagttc gtgtactctt tttccaggat aaaatccgaa
    24961 atctgtgtat ttgacagcgg aaaattcaca ttctgcagca tgtagagaat aatcagtttg
    25021 tataacgtaa aggatgattc aatcacggtg tgtttcctcc tttttcttcg tccagaagct
    25081 gtccctgata cgcatgttct tctttcatgc gatgatgcag ctcatttaag acggtgcgtt
    25141 ttgcctggct ccatgttggt gcaatcagaa gatccccggg gccgtctccc gtcacacggt
    25201 ggatcacaag atcgggacgg aggcgccgca ggcagtcgat gacaagggta agatacgcat
    25261 cctgctccag cacctcaaat tttccggccg cgtagtccgc cgcaagatcc gtgtttttca
    25321 gcacatgaag cagctgaagc ttgatgccgg aaatggaaag tccattcagg taatccattg
    25381 ttgcaaagac ttcttcctgt ccctcccccg gcagaccgag gatcgtgtgg acaatcacgg
    25441 gaatcccccg cctgtgcagc tcgtttactg cctgttcaaa acaggaaagc ggatagcccc
    25501 ttcggatata ctgtgccgtt ttttcatgca tcgtctgcag tcccagctcc acccagaccg
    25561 gtttgatccg gttcagctct tctaacagag aaagaacctc ttcccctaag cagtccggtc
    25621 tcgttccgat cgaaaggacc acgacctcct cgtccgaaat tgcctcccgg aaaatttttt
    25681 ccagatattc caccggcgcg tacgtgttcg tatacgcctg gaaataggcg atgaacttat
    25741 gcaccggccg cttttgctgc aggagggatt tctgctcccg gatctgttcc gggatggatt
    25801 tgtttctgtc cccggcaaaa tcaccggagc cgcccgcact gcagaaaatg cagccccggt
    25861 caccgcaggt gccgtcccgg ttcggacacg tcatcccgcc gtcgagcgag agcttgtaca
    25921 ctttttcccc gaacgtcttt ttcagatagg cgtccagcga ataatacggc tttccaagcc
    25981 agtctgtcat gatttcttcc ccttttattc gatgtctttt ccaatttttt tgaagttttc
    26041 ccggatcttc tgctcatagc cgttcgttcc aagatgaagg aaacggcttc ctaagatctc
    26101 gtccggcaga tactgctgct cgacgtagtg attcgggtaa tcatgtgcat actgatagcc
    26161 ttcaccgtgg ccgagtttgg cagaaccttt gtaatgggcg tcctgcagat gcggcggaac
    26221 cgacgtccgc acacttccga ccgtcttcat cgccgcaaag actgcctcgc acgaagaatt
    26281 actcttcggt gcacaggcaa catacgtggc tgcctccgcc agaataatct gcgcctccgg
    26341 catgccgacg cgttccacgg caagggacgc ggaaactgct acatttaagg cattcggatc
    26401 ggcaaggccg acatcctccg ccgcgcagat catgatgcgg cgggcgataa acttgatatc
    26461 ctccccggca tacagcattt tggccagata aaagaccgcc gcatccgggt cagaaccgcg
    26521 catgctcttg ataaatgcag agatcgtatc gtaatgctga tcgcccgtct tgtcgtaccg
    26581 cacgacgcgc ttctggatgc actccgatgc cgtttcgaga tcgatatgga tttttccgtc
    26641 cgcctgacgt tccgtcgaga ggacgcccag ctcgattgca tttaaggccg ctctcgcgtc
    26701 accgcctgcg atgtcggcga ggaactcttt tgcatccgca tcgagcacaa cgttgtacgt
    26761 cccgagcccc tttaccgggt ccgtcaccgc gcgctccagc agtttttcga tgtcctcttt
    26821 ttcgagcggc tttaattcaa agataatgga acgcgaaatc agcgccccgt tgacctcgaa
    26881 atacgggttt tccgtcgtcg cgccaatcag aacgagtgtg ccgtcctcca caaacggaag
    26941 cagataatcc tgttgcccct tgttgaagcg gtggatctcg tcgacaaaaa gaatcgtctt
    27001 tttgccgtac atgccgatcc gctgctgcgc ttcttttacg acttcttcca tatccttttt
    27061 gccggctgtc gtggcattca gctgggtaaa ctctgcgctt gtcgtgttgg cgatgacgcg
    27121 ggcaagcgtc gtttttcccg ttcccggagg gccgtagaaa atgacggagc cgagtttatc
    27181 cgccttgatc gcacggtaca acagctttcc agggccgatg atgtgctcct gtccaaccac
    27241 ctcgtccagc gttgtcgggc gcatgcgcga tgcgaggggg gactcttttt ccattgtggt
    27301 ttctttcata taatcaaaca aatccatgta tttttcaccc catttttctt tctgccattt
    27361 atcttatcac attttggcgg gtatcctcaa ccccttaatc tcggattctc cgcaactatg
    27421 ttcgatggaa tccgcggaag ctattcttca atcagctcac gcaccgtcac aaacgtgtat
    27481 ccctcttctt ttaattcttt taagatccgc cgcagcgctt ccactgtcgc gcggtagccg
    27541 tcatgcatga gaataatgct gccatccttc acattcggaa gcacctcgct gcagatcgac
    27601 gcggtgttct ggctctccca gtcgcgggaa tccacatccc agagtaccgg gatcatcgtc
    27661 actgcaaggt ccagcccctc tctccattcc ccgaacggcg gacggatata gacgggatat
    27721 tcccctgtga tctcgtagat tgcattgctt gttttcacga cctcctccct cgcttcctct
    27781 tctgatactt tcgtaagctg aacatgatga aacgtatgat ttccgatcag atggccttcc
    27841 gcatgcatcc ggcggacgat ttcttcgttt ccggcaagac actgcccctg cagaaaaaac
    27901 gttgccggaa tttcattttc ttttaaaatt tcaagcacct ccggcgtata gcacgcattc
    27961 ggcccgtcat caaatgtcag cgccgcacgg cggggcacac ttttttctgc ttccgccgct
    28021 gtctctttcg cgccttctct ttcacaccgt gccagctctg caaacagaat agccgccatt
    28081 atcacagcca gaagcggcag aggaacgtgc cgcacttttc ggaatctccg catggtctgc
    28141 ccttcgcact ttcttctgca ttctctgtat tatatgaaaa acaggagaaa agatgccgca
    28201 cccggcggtt cttttctcct gtttttgttc tttatgtatc ctgcacctga agaatatacg
    28261 taccggacgc agtatcctgg taaacgatat ctccgatgga ctgcagcagc gattcgttca
    28321 gcgcatcgcc gtatttctcc ttttctttgc ttcccacaaa aatgtaggtc acatgatact
    28381 gctccagcag gcgcagcact tcattcgtat cggtcgaggt gtacatctgt ttcacgtcct
    28441 cggcgcggac atttaagtct gcgggatccc ctctccagag ccattcatgg acataccatc
    28501 caagcacggt cggaagaccg gtcattgcgg aaacgcgctc gtaatcgctg tagctgtcgc
    28561 cgttcgcctc aagcacaacc ggctgtcctt ttattgtttc atccagccag cggatggcac
    28621 ctgcgtcctc ggaaaagaca ttttccagat acgctgtcgc atcgagtccg cggtactcgg
    28681 acagatcgaa aacatttccg aaccaggagt acacgctcgt tccaaaatag ccgaacgtcc
    28741 ataaaagcag taccagcccg attccgccga acacctgcag aatccgctct tttttccaga
    28801 gcaggaaccg cgtgatgatg tagctcatgc agataccgaa caggataaat gnnnnnnnnn
    28861 natatcccat tgtcattccg aaaaggatat atgcctgata cgtcagcttg aacatcgtgt
    28921 tggagcgcgc atacgattct tcgtagatat cgcgcacata gacgagttcc ggaatgatga
    28981 taaggccgat ggcacagagg cctaaaatac cgatcaccat atcgctcacc gcaatctctc
    29041 tccagaaatt acagaaggca gaaacttttt ttggcttttc cgccgtttct ttttcgcccg
    29101 gctccggttt tttctctccc aggatcaagg caacggcctg ctcttccacc tcttcctgcg
    29161 tctttccatc ggcaagtaca atctgtccct gccgctccat gcccggaaga tgacagtttt
    29221 tccgccatgc tagcagaacg gctgcgataa acagcacaac gagcaccgtc ggaaggcccc
    29281 agagaatcgc cagctgatac agcatcgaat ggtgcttggc aatgccgaca ccggaaacca
    29341 tcgtctcaaa cttcattgta aacggaaggg cgacaatcgt tccaattgca aaagcttctg
    29401 cagcctgcag gagaaccgtt ccgatcgtct ctttcgcccg cgcatgatac cggtacagcg
    29461 caccgtacag agagaagcct gcaatcacga cgaaatagat aacaaaatcc cagtagttgg
    29521 accagtggaa cacaccgatc agaaagcccg ccgcgatgat ctgcggctga agcagaacgt
    29581 cttttaacga ccatttccac tctttttccg gatcacggca ggtatttttc acgtaggaat
    29641 ataaaaggcc cagcaccagc aggacgaaca tgacattcac cacatgggca tgcaggtcgc
    29701 cgagcacaaa ggaatacgat ggaaactcat gaatcgtgcg gtcattttcc acaagcgggt
    29761 catacccgat atagcgggtc gaactcggga accagtaagc cgattcgttc agtcccagcc
    29821 actgtcggat gcagccgtaa atcacatagt gcatatttcc ggcaagggaa acggcgcctc
    29881 ccgaaagaag accgccaagc accggtgcaa tctggctttt attgccgccc tctttccgga
    29941 tgcggtggca cgcacgcgac tctgccagat ggtaggtgat cgaaaacggc agaacaaacg
    30001 caaacgatgc caccatcgta cgcgtcagat ggtacgtctg ctttacatcc gtgaaggtaa
    30061 tcttcgtcag aaatacggca tagaactggc cgccgtaata gtagttaata ccgcttccgc
    30121 tgtaccagag atcctctgcc ggaacggcgg tgctgcgcat catggccttc ataaaaccat
    30181 aatccatgaa tttttcggtt ccgtgtgcct ccgggtgaaa gcccgcaagg taggtccaga
    30241 gaaggaaaaa ggcgaaaaac aacacttctt ccatcatcag gaagtcgatt tccggcgcat
    30301 ggacgcgtac cttaaagcat ccgaaaatcc cagaagccag gatcagtaca ccgactgtga
    30361 tcaggcagac cggtgcggta aacggaacca gtccgaagct tccaagagca aatacagcaa
    30421 aaccggaaac aacgatgcca agcactttag aaaacatcca tccccggtca tgaaaccgcg
    30481 gaaacagcgc tgcggcagcc ggataaaatc caataccgag caccagggtc ataagccacc
    30541 acttcaggaa cgaaacgttg tccgttccaa gaagcggcgt tgtggccagc acaagcaaca
    30601 ccagcagaat gccggaaact gtcgtctttt tgctcatctt taattatctt cctctgtcat
    30661 tgtgtagaca tgacgttttc ttgccatctc atcgcttgca agatactcgt cgtaggtggt
    30721 catcttatcg atcagcgcgc cgttcggaag gatctccatg atacggtttg ctgtggtctg
    30781 tacaaactga tggtcgtggc aggtgaacaa gagcacgccc gggaatttga tcagaccgtt
    30841 gttcagtgca gtgatggact ccatatccag atggttggtc ggctcgtcaa gaacgagaac
    30901 gtttgcaccg gaaatcatca tcttggagag caggcagcgc actttctctc ctccggaaag
    30961 gactctgaca cgtttcacgc cgtcctcacc ggcaaaaagc attcttccga ggaatccgcg
    31021 gacatacgtt acgtccttga tctcggaata ctgggtcagc cagtctgcga tagtcaggtc
    31081 gcagtcgaat tctgcggtgt tgtccttcgg gaagtaagcc tgtgtggtgg ttacgcccca
    31141 tttgtagtaa ccggaatccg gctcttcttc tccggtcaga atcttgaaca gcatggtgat
    31201 cgcctgctcg ttgcttccga cgaaagcaac tttatcttct cttccgaggg taaagcttaa
    31261 gttatccagg attttcacgc cgtctacggt tttggtcagt ccctctacct gcagaacgtc
    31321 gtttccgatc tcacggttcg gacggaaatc gatgtaagga tatttccggc tggacggttt
    31381 gatatcgtcg agctggattt tctccagggc tttcttacgg gaagtagcct gtttactctt
    31441 cgatgcgttg gcgctgaatc tagagatgaa atcctgtaac tctttgatct tctcctcttt
    31501 cttcttgttc gcctctttca tctgttttac cagcagctgg ctggactcaa accagaagtc
    31561 gtagtttccg gcatagagct ggatttttcc gtaatctata tctgcgatct gggtgcagac
    31621 tttattcagg aagtaacggt cgtgggatac gacgataacg gtattctcga agttgatcag
    31681 gaactcctca agccactcga tcgcatcgag gtccaagtgg ttggtcggct cgtcgagaag
    31741 aaggatgtcc gggtttccga acagggctct tgcaagaaga actttgacct tctggctgcc
    31801 ggtcaggtcc gccatctgtg cgtagtgcag ttccggctca atgccaaggc cgtttaagag
    31861 ctgctctgca tcggtctctg cctcccagcc gttcatctct gcaaactcac cctcgagctc
    31921 gctcgctttg ataccgtcct catcggtgaa gtcttctttt gcgtacagcg cctctttctc
    31981 tttcatgatc tcatagagac gggcatttcc catgataacg gtgtcgagta ccggatagct
    32041 gtcgtatttg aagtggtcct gctcgaggaa ggaaagacgc tggcccggtg tgatggttac
    32101 gtctccgttt gtcggttcaa gctggccgga caggattttc agaaaggttg attttcccgc
    32161 tccgttggca ccgatcatgc cgtagcagtt tccctcggtg aatttgatat tgacatcttc
    32221 aaacaacgct ttttttccga cacgaagtgt cacattattt gctgcaatca tactttttaa
    32281 aacctcattt tcttattctt aattgtaaac gtgaagttac cgccggggtt tcccggtcat
    32341 acggagtata gtctaccata ggtttccgtt ttctggcaag tagggaaagc ccttattctt
    32401 cgtctttttc accggaaatt gtaaaaaagc tgtcatcccg gtcttttttg tcgatcagtt
    32461 cattgtactg caggcattca tactgccgtt cgtactcgag atcctcctcc ggcgcatcgc
    32521 tgtcgttcca gcggtaccag gttccgtcac ttgctaaaaa gccgaggtga ttgagcgccg
    32581 gcatcttctc gcgcaggtca agcagatact cgttgtacgg cgtcagttcc aggccggtaa
    32641 ggcttaacgc atagcttgag agatagtttg tgctgatcca gacattgctc tcggatttca
    32701 tgctgtagtt ggtccagatg aagaacgggg ttccatagaa cttctcctga tcctcgaccg
    32761 aaagattctg ataggcgtct ccggcaatcc atgtttcaaa cgcatccggc agtttcgggg
    32821 aatgatcgcc gaacataatg atcatcgtcg gctcgtccac ctgctggaaa taccggatca
    32881 gataggaaat tgcctcatcg gaaagcttta acagggagaa atactgatcg gcctcatcac
    32941 tgtcgtagcc gttgcagttg atatccgcct ggaaataacg gttcgtgtag ccgctgtggt
    33001 tctgcatcgt cacatcaaag aggaagaacg ggctgcccgg ctccttgttc tcgtaggttt
    33061 ccacgatctt ctcatagttc gcctgatcgc tgatcatgcc gcggtaccgc ttggccgtat
    33121 cggagaactg atcaatcgag atgaaatcat caaaatcaag gaaacggtat gccgtgctcc
    33181 gcttccagtt tgtcgccttg ttcggatgca tcgcaattgc ctgataaccc tggtctttta
    33241 acgtcgtgac aagggaatac tggctgtgcg taaagtagct gacgtacggc accatgcccg
    33301 ggaaccgctt gcaggagttt ccggtcagga actcatactc cgtttttgct gttccgccgc
    33361 ccttggtaca cgtcagcgtg tgccctttga tgatattttc ggtcagcgaa tcgaggaacg
    33421 gcatgtagct ctcgctcgtc tgcggatcgc cgacggaacg gtagtcaaac cacgactcgt
    33481 tcatgatcgc gatgatgttg acgggtgtca cgctatccgc attatctttt cgaagatctt
    33541 ctgttttcgt ctctttctcg ctcgtatcgg caatcgctgt cacttcgtct ttcgaatagc
    33601 cgtccggcgg tgtcaggcgc atgtattttg cgaccgcggt aaaaccgacc gtcgttccgt
    33661 actggcggta tgtcttcgcc ggattccaga ggtcactgat gacaccgaac tcggcgttcc
    33721 agttcagatt catgtacgcc aggtatgttc cgagcgtcag cgcagcagca gccacgcgga
    33781 gcagaatttt attcctcgtt ttccgtgcaa aacggtattc ccggctctgc agccagaggc
    33841 gcacaacgag catcatcgtg ataaacgaag tcacgatttc acctgtgatc tcgaatttgt
    33901 agccgccgac tacgtccgcc gccgtcgcaa tgctgtacag gtcgatgagc tggaacgctt
    33961 ctccgcggaa cagataaaca tagtagaata ccagactcat gcagaaataa aagatgttca
    34021 tgaaaatcat cgcgcggcgg atggagttca gaaagaggga aacgaaaatg gaaagtacga
    34081 aaatgacacc gatattcaac aacgcataat accatttcat ctcccggaac tggctgtttg
    34141 tgacaaattc cagtgtggca aacagatagg ctgcgtttgc cagcatcaga atcggctgtt
    34201 tccatcccac atagcgcggt tttggtttct ggtccggctt tgcagcaaac cggcagaaac
    34261 tgacgactgt cgtcagtatg atacacgcca gcaggcacgg gaacagaaag tgaatactcc
    34321 actcccggtc tgccgccgtc gtatccagca gcgggcgcgc cagaaacagc agcaccgcca
    34381 gcacgccgaa atacaaaaca agaaacgcgc accgggcgcg ctccttttga ttctctttta
    34441 atctctgata aaacttctgc atcgaaactt tatgctcctt ttataaacaa gaattataat
    34501 ccttccgtaa acggctcgtc cgccggatag ccgccgccta atttttgcat acagcgctgg
    34561 atggaatctt ttgaatttcc ccagtcctta tccttgttgc ggtaatcgaa tttgtcagta
    34621 aaccatgtta cctcttcctg cagccgctgg taattttctt tcatgatttt gaacagaacc
    34681 gggtaaaatt ccgctttctg tttatcattc agctttttct ccgccgtttc taccagatcc
    34741 gcaaaatgcg gcagacagaa gccttttccc tcgcggaaca gtcttgcaaa ctcctcgtct
    34801 tttttgtaga gatcaaagaa cgtatcaaga tagcggttat agtttgcctt gaagtgatcg
    34861 cagacgtagc acgaatgcgt cttctcatca atccactggc cgatcgcagt ctcacggccc
    34921 tgtttgtcga gggatgtctt ctgcatccgc ttgaacaccg aggattttcc cggtgcaaac
    34981 agatccatct gtgcggcaag ctcctggttc agttttttca gatgggtgga taaaatcaga
    35041 ccgcttccga gacggtttcc gtaatcgtac atcattttat agtggtggcg gcagaatccc
    35101 atcgcgtctg tttccgcacg cacatcatcc tccatatagg atgcgccgga accgagcgca
    35161 aacgaaatcg catgctgctc tgcttcccgc tccagatagc agaacgggca ctcatcttcc
    35221 gccttgaatg catccgtcag cggaatggta tggatcgact ctttcatatg caaaaacctc
    35281 cgattttatt tgttggtgct ttctacagca tacacaaaaa cctctcattt gtcaaaagaa
    35341 ctctttttcc tgttttccta ttattctgat ggataatttc tatattttgc acaatttaca
    35401 tgtgcattat aaaaatttaa agttgtttct ttttggtatt taatcgttta agataaatat
    35461 tatataagtc tttgtatcac tctgttcaga aaggagccat agcaatggga gaacgtcata
    35521 cggaggagat gcgcctcctc ggtcttacca tcgcttttta tcgcagagca cgcggattaa
    35581 cgcaaagtga gcttgccgaa gctgtccaga tcagccgtac acatatgagc aacatcgaag
    35641 cgccaaacag tcatacctct ctgtctctaa acaagctgat ggatattgcg gaagcactcg
    35701 aaattccgct gaaaacatta tttgatttca aacggtaagc ccggaaagct ttccgttttt
    35761 tatagcagat ttttacagca accccccgca gccggtgtgc gcccggtgcg gggggtcttt
    35821 ttctctctta ttttcccgaa aaatccggag ttccgtcaag attatacggg gtcacctggt
    35881 atacatagta gttcagccag ttcgtgtaca gattgttcgc atgtgaccgc cataaaagca
    35941 ctggttttgt atttggatcg tcgtttttat aatagttctt cggaatctca atcgggagtc
    36001 ctttgtcttt atcgcggtgg tactcgccgt cgagcgtcat acggtcgtac tccggatgac
    36061 ccatcacaaa aatctttctt ccctcttccg ccatgccaag atagaatccg gcttcgtcgg
    36121 actctgcgag aatcgtgaca tctttgcagg cacggatatc gtcgatcggt acttctgtgt
    36181 ggcgggaatg cggtgccaaa aactcgtcat cgaagcctcg cacaagcggg atcttgcggt
    36241 tgttgacttt gtgccagaac agcccgaatt tcttctccgg aagcatccgt ttcttcagtc
    36301 cgtagaaatg atacagactc gcctgggctc cccagcagag gaacatcgtg gaggtaacgt
    36361 gtgtgttcgt ccagtccatg atctttgtca gttcatccca gtagtcaact tcttcaaatt
    36421 ccatctgctc gaccggtgca ccggtgatga tcataccgtc gtaataattt ttccgcacat
    36481 tctcaaattt cacatagaac gtgttcagat ggctggtcga tgtatttttc gcctcgtggc
    36541 ttgccaccat cagaaaggtg acatcaacct gcagggaggt gttggaaagg gagcgcagaa
    36601 gctgcagctc ggtatcttct ttgagcggca tcagattcag gatcataatc ttcagcggac
    36661 ggatgtcctg atgaaccgct cttgtctcgt ccatgacgaa tatattttcg ctttcgagta
    36721 tttcttttac aggtaaatct gcctgaactt taaccggcat attctttttc ctcgtttcta
    36781 ttcttcttga tctgccgcgc ttttctctgc gcagacaggc ggtttgcttt ttccggcagg
    36841 aatttctgcc gtctgtttcc gattatagca gattcccgcg cgggatgcaa accgcctttg
    36901 cagatatttc gttattcttc tgtcatcttc agaaattcct cttctgagat caccggcacg
    36961 ccgagttcgc gcgctttccg gtttttggag gaactggatg tcgtgtcgtt gttgatcaga
    37021 tagctcgtct ttcccgttac acttccggtg gcttttccgc cgagctcctc gattcttgcc
    37081 tttaatgcat tccggttcgg gaaatgttcc acacttccgg tgatgacgaa ggtaagaccg
    37141 gctaatttct gttcttcctg gcttgcctgc ggcttctcaa tcgtcagata cgacagaagg
    37201 cgctcaaggc gttctttatt ttcctcatcc gcaaagtaat ccacgatgct ttttgcaatc
    37261 acttctccga caccgtcgat ctgcgcgaaa tcctccgccg atgcattccg gatccggtcg
    37321 agatcgaaat cgaaggcttt gcagatcatt ctcgcgtttg caagaccgat atttaagatg
    37381 ccaagtccga aaatcagacg gggcagcgtt gtgtttttcg cgcgctcgat gctgtcgatc
    37441 agattctggt atgatttttc accgaaaccg tccatctcca caatctcgtc gcggtagcgg
    37501 tcgagacgga aaatatcggc aaactcacgg atgaagccgt ggcccacgaa tttttcgagc
    37561 gttgcctccg acatgccgtc gatgttcatc gcgtcgcggc ttacaaacag ggtgaatgcc
    37621 ttcatgaatt ttgccgggca ctccggattc gtacagtaga gggtctcgac attattcatc
    37681 atctgaatcc gggtggcacc gccgcaggca ggacacgttt ccggaatatc gcgcacgccg
    37741 cttctcgtca gattctctgc gatctgtgga atgatcatgt ttgctttgta gacaaggatt
    37801 ttatcaccca caccgagatc cagcgaacgc atgatactga tgttgtggac gcttgcgcgg
    37861 gaaaccgaag ttccttccag ctcgaccggc tcaaaaaccg caaccggatt gatgagaccg
    37921 gttctcgacg ggctccactc aatttcaagc agagtggtct ccttctgttc atccgcccat
    37981 ttgaaggcga aagcgttccg cgggaatttc gcggttgtgc caagtgattc tccgtaggcg
    38041 atatcgtcat acaacgcgac aaggccatcg gacgggaaat cgtttgtctc gattttcgtg
    38101 gagaaatatt gcatcgcctc gtcgaggtta tctctggtga caaccttgta ttccaccacc
    38161 tcgaagccct gctctttcag ccactccatc tgacggattc tggaattttc gaaatcctgt
    38221 tccggagcgc tcacaagcgc aaatgccata aaatgaacgt ggcgttttgc cgtaatctca
    38281 ttgttcagct ggcgcacaga accgctgcag aggttcctcg gattcttgta tttcgcatcg
    38341 acatcctcaa tctcctcgtt gatcttctca aaatcggaat agcggatgat cgcctcgccg
    38401 cgcagaacca gctctccctg gtacgggatt tttaagggaa tattttcaaa cacttttgca
    38461 ttgttcgtga caacctctcc gatcacgccg tttccacggg tgacggcctt tgcgagggta
    38521 ccgccgcggt acgtcagaac gacggtcaaa ccgtccatct tccaggatag cagcgttttg
    38581 tgatccccga tgaagctgcg gagcacttcc acatctttcg ttttatcaag ggaaagcatc
    38641 ggcgactcgt gcgcttcttt cggcagttcg tccatcgcct cgtagccgac ttttgtggtc
    38701 gggcttccgg cgagtgttac gcctgttttt tcctctaata cctgcagttc gtcatacagc
    38761 gtgtcgtact ccaggttgct catgatctcc cggtcttcct ggtaataggc ttttgccgct
    38821 tcgttcagct ttttgaccag ttccttcatc cgttctgtct cgttcatcgc ttttcctccc
    38881 cgatctgctt tttcagttct gctaattctt tttccgttaa agggcggtac tcccctgttt
    38941 tcaggtcttt caagtgaata ttcatgatcc gcacacgttt cagctttgtg acacgatatc
    39001 ctaatgcttc gcacatccgc cggatctgac ggttcagccc ttgcgtcaga ataatcgtaa
    39061 agctgcgctc tcccgttttc cggatccggc acgggcgcgt cacggtatcg agaatcggca
    39121 cgccggatgc catttttttc agaaattccg gtgtaatctc tttgtttacc tcgacctggt
    39181 attctttttc gtggcggttt ctggcacgca tgataccatt tacaatcgca ccgtcgttcg
    39241 tcatcagaag gagaccctcg gaatctttat cgagtcttcc caccgggtaa atccgcaccg
    39301 ggtaggacag ataatcgatg acgttgtttt tctcccgttt ttccgcagta cagacgatgc
    39361 cgcgcggttt gttgaaggcg agatagactt ttttctcctc cgggacaacg gttttgcctt
    39421 tcacgacaac aacgtcgcca gactccacct gcatgccggt ttccgcgcgt tttccgttga
    39481 cggtaatctc tcccgcttct gtcagacggt ccgcctcccg ccgcgagcag acgcccgcat
    39541 cacttaagta tttgttcaag cgcatggttt tatccttcca aaaaagcagt ttcataaaaa
    39601 ccagaaactg ctttttttgt tttgtattat tctattgttc ctactgaatg ctctctacat
    39661 atgctctcag ggaagcatct gcatcgagtg cattctgata atcgttctga acctgttcga
    39721 tcagattttt gacttcttcg ttttcgagcg ccgctttcat agcctcggtt acggccgcat
    39781 cgctgtcggt atcatccatg attttcaggc tgccgtcttc tttcgtgtag agataatact
    39841 ccgaaagcat cggcagggtc tggttgattc ccagatattt gcatctgctg ctgacaaacg
    39901 tgatgtacgt tccgtcgctt ggaccgctga tcgtgtaagc ctttacatct ccgtatgact
    39961 ccacgtcgct ttctgcctca atctgcgcct gctcctccgc ggtcagatta tctgttactg
    40021 caaaaagacc gttgatatcc cttgcaccga gcgcggtgta atactgctcc agcacggtgc
    40081 ttacttccgg gacattgtcc gcggcaaagg agacagccgt attatcggta ttctctgtat
    40141 tactggtatc gtctgccgct tccggctcac ttgtcggctc agcctctgca tccggcgcgc
    40201 tggcggtatc ggtactggaa gcatctgcac tggaatcatt cgcggaatcc gaaacgtccg
    40261 atgaaatgcc tgtgctgtcc tcagaatctg ctttgctgtc tgcttttacc tgtgtctttt
    40321 tatccggcac attgtttccg gtgctgctgc ttgttacggt tccgccgctc gattttccat
    40381 cgttttttgg tgataccaga tgaacgatga gaagaaccgc tgccacaatc aggagaattg
    40441 ctccgatcag aagcatgtag cgcagattat cggaaatcca ttcccggatt ttatcttttg
    40501 ttgtcattgg ttttgccata tttttcctcc ttcatcaaaa ctgatccgcc ggatcagtta
    40561 ttgatatgct cagtaagcaa aaaaacctct acttcacacc gaagcagggg ctttccttgt
    40621 cctgcgccgg tcctgcccgc cgcagcatgc catgtagcaa aaacgcgtct ggagggattc
    40681 gaacccccga cacacggtac cggaaaccgt tgctctatcc cctgagctac agacgctgat
    40741 gtgatctgtt ttctcaacca cactatgata gtttaccaca gactcctgca attgtaaagt
    40801 atttttccca cccagccgcc catttttttc ttaaattatt cgcagactat tctgccacac
    40861 agcttacgtt tcttcctcac acagcgtcag ttccccgctt tcccggtggt aataataaac
    40921 gtgatccgac agaatctcct gcggcttgac cgtcttttca ttcacgtccc gtaccatgtc
    40981 ggaaagacac tcctccggga acacatcata atccggaatc accatgcact cgtggatact
    41041 gctcggaatc acataaagac ttgcctcaaa gcgctgcgaa agactctcca gcacgccggg
    41101 atagcacatg cagattgcac ccagctttcc tctgcggttg ctgaccaggt aaaactgttc
    41161 cgccagcgga ccaagctcga acaggggaaa cgttactccg tactcctctt tcaaatacac
    41221 cataaggctg tctgtgcgca ccggcagatc ccgcagggtg ttctcccagg cacgggcatc
    41281 cagctcctcc gccgaaattc cccacagctc ccggtgcgtc tcgtggatca gaattgtcgc
    41341 atccggcacg cggtttcccc gcatctggta atagtacacg accgcgagat cgagaaattt
    41401 tctgtgcgga acttcggaga gcagcgtacg gtttttctcc gcatgaatga gctcgcagta
    41461 gatctgcggc gcagcatttt tccagttggt aaaaaaggca gctttctccg gtcttctcgc
    41521 ctccgcttcc actttccggt actgctccag taccttttct gcaatctgag aaagcggcac
    41581 gccctgcaga cagcattcat acagaagctc cacgtagatc gtcggtgcaa tccagtcctt
    41641 ctttctgctg atgacaaacg cctctttttc gataccgttc agcttctgca ccgattctac
    41701 cttcacctca aaatcatcgc cggcttttct tttcagcagc gcggcaagct gctctttgaa
    41761 catctgatag tccatttttt cccacctttc ctgtttgatt gccttttgta tttttgcttt
    41821 acggaacaaa aaagctccgg aaaacacaac ttaaaaacct ctgtacagat ctgtatatgc
    41881 cagagattcg ttggttcgcg gaacttcgaa cgatgagaaa aacgggcccg ggctgcttcc
    41941 gacagccttt atcagccatt tttcagacga atattctatt tatttagaaa accatgtccg
    42001 ccacaaagaa agttgtgacg gacaacggcg ggaacagtct tacttataca cgggacagat
    42061 attcacctgt tctggtatca attttgattt tgtctccctg gtttacgaac agcggaacgc
    42121 ttacggtagc accagtctca acggtagccg gtttggaagc accctgtgcg gtatctccct
    42181 tgaaacctgg ctcagtctcg gtgatctcaa gctctacaaa cagaggcggt tcgattgcga
    42241 atacatttcc gttgtaggaa catactttaa ccatttcgtt ttcttttaca aatttcagag
    42301 catctccgat ggtctcttcg ttcagagcga tctgctcata gttttctacg ttcatgaagt
    42361 agaataaatc accatcgctg tacagatact gcatgtctac acgctcgata cgagcctgcg
    42421 ggaatttctc agtcggacgg aaggtacgct ctaccacacc accgttgatg acatttttta
    42481 attttgttct tacgaatgca gctcctttac caggttttac atgctgaaat tcaagaatct
    42541 gaacgacctg accatcgatc tcgagggtaa cgccgtttct aaaatctcct gctgaaatca
    42601 ttctgatttt tcctccttaa catagtacga tgcgccaaat gcgcaccata ggcttatctt
    42661 ataatataat atttttcccg atttttcaac tgtttttttt cgtaatcctg ccgcgggcca
    42721 gatcacgctc ataaaagaac agtacaatgt gctccagata ccgtttcaga acgatctgaa
    42781 tttcctcttt cggattgatc ggctcggtca gaatattgta aagtccggtc cgttttgcac
    42841 cgtacacatc ggtgaaaagc tgatccccga caaagaccgc ctgctcctct ttgatcccca
    42901 ttctccggca tgcctcctgg tagtttttca ccgaaggctt gtgcgcattt tcgatataga
    42961 tagaaccgac cgcatcggca aacggagcca cacgcggttt ctgattgttg gaaagcagac
    43021 aggtggaaaa accagtctcc cgaagagacg caaagaacgc agccgcctcc ttcgttgccg
    43081 gcgcaccgtg cggaacaagc gtattatcga tatcaaaaat cacgccccgg tatcctttcc
    43141 ggtacagatc cgcgtaatcg atcgtatagg cacttttttc tctccggtcc gggtaaaaca
    43201 tccgcatcag tatagttccc cggttcttct ggttttttcc actcatcttc tttctccaat
    43261 cctcttttcg tttatacagt aactttattc ttctttcaga aactcctgca gagcctcaaa
    43321 atttgctttc atctggtcat agccgtgctg gtaaaattcc cgcagcttct cctcgtcctt
    43381 ttcgagacgc gataccgccg gtatctccgg gcggatcaca aagacggagc cttcttcttc
    43441 ccatttttcg atgcattcga gcacctggtt ataatgtttc gcgcggttta caagcgcacg
    43501 cacgagattc gggtatttgc ggaatgccgc cacatacagc tcccagcctc tcgttccctc
    43561 tttttttcgg taaccgaaat tccgtgtcag aataatgaca ttttttcggt gtcccgtctt
    43621 taaggaacgg ataatcggga tcgaatcggc aacaccgccg tcgacgtact cgtttccgtc
    43681 gatctccacc atcgggcagg cgatcggcat gctcgatgat gcacggccaa ttgcaagcag
    43741 acgttcttta ttttcgcggt catccagata ttccgcgcgt cccgtctcac agtttgtcac
    43801 aaccagctca cacgtctgcg gcgatgcaaa atacgtatca aaatcaaatg gaaaaatctc
    43861 atacggatac cgttcgaaca ccatatccat gtcgagcagt gcttttttct caacgattgt
    43921 cttgatattc ttattgacgt agcggttttt ctcatccgtg acaatcatac agtccctcgt
    43981 tctgccaatc tggcgcgata catagtccag cgcattgcag gctccggcgg aaacgccaat
    44041 cacatgaggg atataatact cctgctccat cagaaagtcc agcgctcctg ctgtaaagac
    44101 gccgcgattg gcccctcctt ccagtatgac tgctccggat tttgttatgc tgtaattttt
    44161 tgtctcattc attctcctgc actctcctat ttttcacaat gacacattac ttcctgcaca
    44221 gagcaatgcc ggagcagatc ggaaaatccc gacacgctcc ggcccttctg cctgttttat
    44281 ctgttttcca gcggaacata gtccacttca tctttgacac tcttgtacgc cgggcggatg
    44341 atcttgtcgg tgttgatcag ttcctccatc cggtgtgcac tccagccgac gatacgcgcg
    44401 caggcgaaca tcggggtgta gagctccagc ggaagatcca gcatgctgta aacgaagccg
    44461 ctgtagaagt cgacgtttgc ggatacacct ttgtaaatat ggcgctcctc gccgatgatc
    44521 ttcggagcca gttcctcgac catagcgtac agccggtaat ctttctctct gtgtttttct
    44581 tttgccagct gctcaacgaa gctcttaaag atgcgcgcac gcgggtcgga aatggagtaa
    44641 accgcatggc ccataccgta aatcagacct ttctggtcga acgcctcttt gtgcagcagc
    44701 tttcttaagt aattcgatac ttcttcctca tccgtccagt ctttgacgtg tgacttcata
    44761 tcgtcgaaca tccgcacaac cttgatgttt gcaccgccgt gcttcggtcc ctttaaggag
    44821 ccgagcgccg ctgcaattgc ggagtacgta tcggttccgg aagaagaaac aacatgtgtt
    44881 gtaaaggtcg agttgttacc gccgccgtgc tccatgtgga gaacgagggc aagatcgagg
    44941 atcttcgcct ccagcggagt gtacttctga tccggtcgga gcatcagaag aatattttct
    45001 gcagtcgaaa gcttcttcga cgggttgtgg atatataaac tttttccctt gatataatgg
    45061 ttgtacgcct gatagccata gacggacagc atcgggaaaa cagaaatcag gttaatgcac
    45121 tggcgaagca cattcggaag ggaggtatca tctgcgtggt tgtcgtacgc gtacagggtc
    45181 agaacgcttc gggacagagt gttcatcatg tccttggtcg gcgctttcat aacaacgtca
    45241 cgcacaaagt tcttcggcag ggactgctgc tgttttaaga ttgtcttaaa atgattgagc
    45301 tgctcttcat tcggcagatt tccaaataag agcagatacg caacttcttc gaatcccata
    45361 cggttttcac ttaaaaagcc ctgggtcagt ttttccacgt tgatgccgcg gtagtacaga
    45421 cgtccgtcac acggcgtttt gacgccgtca atgatatccg aagacacaac gtttgaaatc
    45481 tgtgtcagtc ccgcaaggac gccaacgccg ttgatatcgc gcagtccgcg ttttacatcg
    45541 tattgcccat acagcgaaac atccattttg gagttatcca gacagatgtt tgtcagttcc
    45601 tctatttccg gtgttacttt catgctaaaa gccatatgca ttcccctttc tcttctctct
    45661 ttttacttac tttcactcat tatagcatta aaccagggga ttcgtatact gaaattttta
    45721 taaacattcg gaaaattttc gtaagatttt gtctcacaaa aggtatcagg cttctgtttt
    45781 acatgcatcg aaggcttgat caagcgcgtc caggaagtct cccatcacgt aatacagcgt
    45841 ctcaaaccgc agataatcga ggacgttttc atcccacata aacttcagca ccggcgggaa
    45901 ttcctcatcg gactcccaga actggaagac gagcggcaga aacggaaaga catgaaaaac
    45961 aaaagcagcg tctcccgggg aatatggttt tccgctaagg gaaacgcatg cttttttcag
    46021 ctgttccaca tgcccgtcat accgtgcggc gcggctgtcg tgaacgccgg acgcaatcgt
    46081 tcggcctttg atcgttccct ttaaggaatt ggtgcgcgca tacgtgccgg cgagcacaat
    46141 ctccttcttt ccgcagcaga gcaggtcgaa aatcgtcagt gtctcattga aactgccctg
    46201 gtgcacatga agaaaatggt cttttgagcc ctctacaacg ccggtatggc ggttcactct
    46261 ccactcctca aaaaggtacg gcacatagaa ataatcgtca tcaaaggcaa agccatactt
    46321 cccttcgatc tccttctgat cgtacgtcag aaagcgctcc tgcatctgat cacgcatttt
    46381 aatatagttc gacacttcca tttttttgcc tcccgctatt tgtcgagcat ctttttcagc
    46441 tcgtccatga acgtgttgac atccttgaac tcgcggtaaa tcgacgcaaa acggacgtac
    46501 gccacctgat ccaggtcttt taagtaatgc atgaccttct ctccgatcac agagcttgga
    46561 atctcgcgtt cctcgccgtt gaaaagctcc gtctccacct tgttgaccat ctcgttgatc
    46621 ttctcggcgg acaccggccg tttgtggcag gcgcgcagga tgccggtctc gattttcgag
    46681 cggtcatact gctcgcggtt catatctttt ttgatgacga tcaaagggat cgtctccact
    46741 ttttcgtatg tcgtaaagcg tctgccgcag acatcgcaca tccggcggcg gcggatcgca
    46801 ttgctgtctt caatcgggcg tgaatccaca acccgggtat tatcctgatt acaaaatgga
    46861 catctcatat ctttcgtcct cgtttctaaa taagtatttc ggaaagtttt ctacttttac
    46921 agtatacgca tttttccgtg aaattcaagt caactgcagc aacattttcg catatgtttt
    46981 tccataggaa tctgtaaaaa ggagcaccgc atgcgcgttg tagatctccg ccagaaagaa
    47041 gttatcaata tctgtacctg caaaacgctc ggctgtccga ttgatgtgga attcaatcca
    47101 aaaacgggac atcttaccgg actcgttctt ccgggtcctg tgaaattctg cggctttttc
    47161 tgccacggcg aggattttgt gatcccatgg gaccgcatct gccagatcgg caacgatatc
    47221 attcttgtcg aaattccgga aaaagagaaa aagcagccct gatcaacgga tatgaaaacc
    47281 gaattccgat tccagcggaa tcttcgtcca ttttgcatcc gtgatttcga tgtagctgga
    47341 ggtgcggatc cttttgatca tctccgtgat cgtctcctca tcaccctgca cctccatctc
    47401 caccgtctca tcccagcagt ttctcaccca gccggtcagt ccgagctggt aggcagcata
    47461 atacgcccgg taacggaacc ccacgccctg cacctggccg tagaaaatca catggtaccg
    47521 gatttcttct ttttcgtctc ttctgtctcg tttctctgtc tcgttcatat ttctctgcct
    47581 cccaacagtc attgcctaaa attcgtctgc cgtcgcacag tacagcggaa gcgggaacca
    47641 gtcggatgga atgttcttcc agatttcaga tggaaccttg cttcccttac ttgccagcgg
    47701 tgaaagaagc agttcctgcg cctgctcccg gttgagtaca tctgcccccg gatgggcttc
    47761 ccgtgtcacg gtcacagttc catcttttac cgtcacggtc agcggctgtc cctccagtac
    47821 ggcgaaccac gttccatcgc tgatgcccat cgtctctgct ttcatggtca gcattgcctc
    47881 gatgacgtct gcaaattgga aaatacggta catcgcggaa ccgcagcgct cttttgtgaa
    47941 gctttcgcag acttccgata acgctgctgc ttttccggtc tcatacaccg gaatggaaat
    48001 gaaaagagag tcaagcgcat tttcttttaa ataagcggca agtgcaggaa cgaaatcctg
    48061 ttcctccagc acttctgctt ccacaacctg gtttccgccg caaagtgcca gatatccgat
    48121 ccgctcaccg tttttccaga ttgcaattgc ctgctcgccg aacgttttca ggcaggcttt
    48181 cacatcctct cttaccacgt aaagcggttt ttcctcattc agcttctttg caaaggctgc
    48241 cgcctcttcg tccgcttcgc cgttttccgg aaacagcggc gcaaaggtgt aagcggagag
    48301 ttccccgttt ttccaggtat ggcgcacatt tgccacatcc aggtaaaacg tgtatttttc
    48361 acttcccggc gtaaagccaa agtaggcata ccgctgtctc tggccgtcca gtgccagaag
    48421 atcacacgtt ccttccagat ccgtaatcca gtcgccaaga agacgcttca tgtggccttc
    48481 gccccttgcc tttggatgca cggaaacgct tcccacatat cctgcgcgga gcagcttacc
    48541 ccctgctgcc atttgctgtg gaagcaccgc aatctcggcc cggagtctgc cgttttccgc
    48601 aacagcgacg cggtggatcc gtgccatttc cggtcctgcc tgataaaccc ttggaattaa
    48661 ctgctcaaag tcgtgcggac ggtgtgccgt gctgaacacg tagtttgcaa gttcgatgca
    48721 gtcctcccac tcttccggct ttgcaagtct gtactctgtc ataatgtaaa aacctcctga
    48781 tcctaaaaac ccttttgttt ttgctcttct ttttctctat cctaacggat ttttgtcctt
    48841 ttgtcacgaa aattccgaac aaattgcaat atttttgcta tatctggtaa gtcatgacca
    48901 cccgcttagc gggtggcatg ttcnnnnnnn nnnttttgct atatctggta aaaaaaagaa
    48961 acgcgcccgg aaattttcat tccaggcgcg tttccctttt ctcaatgttt cctgttttac
    49021 tggattcctt ttacggtgta atcctgtgcc tctacggctg ttttcagcac atcgtctgat
    49081 accggagctg tcagtgttac aacggctgtt cctttttcat ggcttacttc tgcggatgca
    49141 acaccctcga ttgcctcaag tgttttcttc actcttgcct cgcagtggcc gcacatcatg
    49201 ccctcgattg tcatggtttt taccattccc tgttctgttg tcatgttcag atcctccttt
    49261 ggaatttcgt ttttgattct tttatctttt gacgcgtcgt gcatccgaaa cagattcaga
    49321 cgcagggcgt ttgtcacaac gcagaagctc gaaaggctca tcgccgccgc accgaacatc
    49381 gggttcagct tccagccgaa cagcgggatc catacgcccg cagccagcgg aataccgatg
    49441 atattgtaaa tgaaggccca gaacaaattc tcgtgaatgt tcctgagggt cgcacggctt
    49501 aagcggatcg ctgccggtac atcggagagg cggctcttca tcaggacaac gtctgccgca
    49561 tcgatcgcga tatcggttcc tgctccgatg gcaataccga tatccgcact ggtcagagcc
    49621 ggtgcatcgt tgatgccgtc tcctaccatc gctacttttc ctttttcttt caggcggctg
    49681 atgaccgctt cttttccatc cggcagaacg cctgcgatga cttcgtccac acctgcctgt
    49741 gcgccgatgg cacgcgcggt gcgctcattg tctccggtca gcatcacgac acggattccc
    49801 atattctgca gttctttcac cgcacgcggg ctgtcctctt tgatgacatc cgcaaccgcg
    49861 atcataccga gcatctttcc gtcttcactg aagaacagcg gtgtctttcc ttcttcggaa
    49921 agttttgctg cctgctcttc catctgtttc gagagaaccg cttttttcgt aatcagtgcg
    49981 cggttgcctc cgcagagctc ttttccgttc cgttttgcca caaggccgtt tccaggaagc
    50041 gcagagaaat cactgacttc ttcgcgggcc aggccttttt cctctgcaag tgcaagaatc
    50101 gcacgggcca gcggatgctc acttttctgc tccagtgcaa aggcaccttt taaaagctcc
    50161 tcctcactta caccgtccgc cgggacaaga tcggttacct gcggctctcc gcttgtgatg
    50221 gttccagtct tatcgagtgc aacgatctgt gtttttcctg tcttctcgag agaaactgcg
    50281 gttttgaaca tcgttccgtg ttttgctccc attccgtttc cgaccatgat cgcaaccggt
    50341 gttgcaaggc cgagcgcaca cgggcagctg ataacgagaa cggaaatacc tcttgcaaga
    50401 gcaaagccga cgctctctcc tgcaaacagc cagatcacaa tcgtagcaag tgcaatgcag
    50461 ataacggccg gcacaaaaat accggaaact ttatcggcca ctttggcgat cggcgctttt
    50521 gtcgctgccg catcgctgac catctgaatg atctgggaga gtgttgtgtc ttctccgact
    50581 ctcgtcgcct cacaggtcag gaaaccggac tggtttaagg tcgccgccga tacgcggtcg
    50641 ccggcctctt tatccaccgg aatactctcg ccagtcagcg cggattcgtt gacggcactc
    50701 gttccctcca ggacaacgcc gtccaccgga atattttcgc ccgggcggac gatgaaatga
    50761 tcgccctttt tcacctgatc aatgccgact tccacttctt ttccgtctct taagagaacg
    50821 gcggtcttcg gagccagctt catcagactc tttaacgcat cggtcgtttt tcctttggaa
    50881 atcgactcca gcatttttcc gacggtaatc agggtcagaa tcatggctgc ggactcgaag
    50941 taaaattcat gcatatagct catgactgca tccatgtccc cctgtacctg cgctcccgtc
    51001 atggcaaaca gggcgtagac gctgtataca aacgatgcgg tagaaccaag cgctaccagc
    51061 gtgtccatgt tcggtgcttt atgccagagg ctcttgaaac cgctgataaa gaatttctgg
    51121 ttgatgacca tgaccgcaat ggtaagcagg agctgtacca gtcccattgc gatgtgattt
    51181 ccttcaaaga atttcggcag cggccatccc cacatcatat ggcccatgga gaaatacatg
    51241 agcggaatca aaagaaaaac ggaactcacc agacggcgtt tcatgaccgg tgtttccttg
    51301 tctttcagca tctcttcgta ttctgctgtg gaagccgact gtgatgtgct tcccttctcg
    51361 ccttttttcg atgcaccgta gcctgcattt tccacggcgg caataatctc tgccggggag
    51421 gccgttccct ccacgcccat ggaatttgtc agaaggctga ctgcgcagga tgtgacaccg
    51481 ggtacctttg acacggcttt ttcgacacgg gtctggcagg cggcgcagct cataccggtt
    51541 acattgtact gttccatcgt atgtcttcct ttctgtagtt ctggattttc tttttcgtat
    51601 accccaatag ggtatcttat tttcttctac actatatacc ctgtccgggt attttgcaag
    51661 tgttttttca aaaaatttcc acttctcttc tgccatcccc cgcacaatcc ggccggacca
    51721 tcctcagaaa tcccttgaca acccggcgca tgtattttat attgaagcaa tcatggatga
    51781 ttttttcttc ttcatccttc ggaggtt
//
LOCUS       DAOMUF010000002        50262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_42_length_50262_cov_4.151792, whole genome shotgun sequence.
ACCESSION   DAOMUF010000002 DAOMUF010000000
VERSION     DAOMUF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 50262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50262
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42_length_50262_cov_4.151792"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1934
                     /locus_tag="PLN77_00250"
     CDS             234..1934
                     /locus_tag="PLN77_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM42741.1"
                     /translation="MKKRHVMRALVAAATALALTACGSTGSSDTSKSTSTASAASTSS
                     GSEADTENTITIPAGESVSQDSDVTAMVAVDFTTMDPMDTSDTLSGGVQRMMMDGLFG
                     FDENMQIIPMLATSYEANDEATEYTIHLREGVSFTDGTPWNADALIANVNKWADKSLG
                     LKRTTFLCNVLDHAEKIDDYTVKIYLSQSFGAFISNLAHPATLIMSPKQIEAGEDACA
                     QAPVGTGQYKFVEWVAGDHMKVELNKDWWGYDADVCGGTALADADAGFKSITFKPVAE
                     SATRVSAIQAGDAQIMWSVPTESVDTLKGDSNVSVGMGDSIAVWYFFMNTQKAPFNDV
                     KVREAMAYAINKEAYIQVVMNGYGSVATSMVGEEVQHYKGNDPYSYDPEKAKELLKEA
                     GYPDGFTTTLMYSNTTANQKKAEFYKQQLSEVGIDLELNGMENAVLNEKVQGADCAGA
                     DAEVDCYLSGWSTSTGDADWGLRPMLATESEPPMSYNVSYYENEKVDQLLKDGLATAD
                     EDKRGEIYGEVQDIVWKDLPLLCIASDKNIWATSNNITGVYLLGDGSIGMRNARMAAE
                     "
     gene            2075..2995
                     /locus_tag="PLN77_00255"
     CDS             2075..2995
                     /locus_tag="PLN77_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM42742.1"
                     /translation="MPKYILKRIVGTIPTLIIVVTFVFFFVRLIPGDPARLVAGEQAT
                     QDDVEAVREELGLNKSIPAQYVTYVTGLLKGNLGTSLRTKQPVTKEVAGRFGNTVRLA
                     FVTLLWSSVVGVLFGVWSGTHRGKWQDYTGMTIAVSGISLPSFWIGFLLIMLFAVKLR
                     ILPTTSGTSLKSLIMPSITLGVGIAAVIARFTRSSIIEVLKEDYVRTARAKGIKEKVV
                     IWKHVFRNSMISVVTVVGLQFGFLLGGSVVTETVFAYPGLGSLLIESVNYRDYPAIQS
                     LILIFSLQFIVINLIVDILYAVLNPEIKLQ"
     gene            3008..3892
                     /locus_tag="PLN77_00260"
     CDS             3008..3892
                     /locus_tag="PLN77_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM42743.1"
                     /translation="MKEKKKVQTENQERIKTPFSEMVRKFKKQKNAVVALCFIIFLIF
                     LAFFGSKIVPYGINEYDYNAILQGPSMKHLFGTDEFGRDLFSRIVCGTRISLAVGFGA
                     VTVGAVCGTILGLLAGYYGGWIDSIIMRICDVLFAFPGLILAIAIVAILGSGMTNVVI
                     AVAVFSTPTFARLVRSKTLTLKNSVFVLAARNIGVSDARILFHHILPGAIPDIIVQYS
                     MSFGSSILTASSLSFLGMGAQPPTPEWGLLLSNGRNYMTSAMYVTIFPGLAIFLTVLS
                     FNLLGDGLRDALDPKLSD"
     gene            3908..4921
                     /locus_tag="PLN77_00265"
     CDS             3908..4921
                     /locus_tag="PLN77_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM42744.1"
                     /translation="MATDNVLEIKNLHTYFYTDGGVIKAVDGVDVELRRGATLGIVGE
                     SGSGKSVTSLSVMGLLTGTKGKIADGEILLDGDDIVKLTKEQQRKLRGSKISMIFQEP
                     MTSLNPVMKIGDQVAECVLQHEKISKKEALKKAEDMLRKTGVPRVEHMMKEYPFQLSG
                     GQRQRVMIAMALVCKPEILIADEPTTALDVTIQAQILDLMNQLKKETGTSILFITHDL
                     GVVAEVCDDVAVMYCGRVVERGDVKTIFANPSHPYTKGLLGSIPRLGDAGKELKSIPG
                     NVPNPKYMPKGCKFEPRCPYASEKCREEEPGFFQIEEGHISRCWLCESGQLKTDHVEK
                     AGD"
     gene            4923..5915
                     /locus_tag="PLN77_00270"
     CDS             4923..5915
                     /locus_tag="PLN77_00270"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRM42745.1"
                     /translation="MSDTLLRVEDLKIYYPVAGSGFGKKEFVKAVDGVTFEVKKGEVF
                     GIVGESGCGKSTLGRGVCKLENLTSGHVYLDGEDITEYNDRRMRSIRKKVQMVFQDPY
                     ASLNPRMSVFDIIAEPLLVHHLYQDKADLEKKVLDLLHRVGLDDYHANRYPHEFSGGQ
                     RQRIGIARALAVEPSLIIADEPVSALDVSIQAQVLNLLNELKHDLDLTYIFVAHDLSV
                     VEYISDRVGVMYLGNFVEVGEKEKIYSNPMHPYTQALLSAVPVPDPTAKRERILLEGS
                     IPSAHKPPTGCKFHTRCPKCMECCKTQAPERYEVDDGHYVYCHLYDKERRKQQK"
     gene            5912..6712
                     /locus_tag="PLN77_00275"
     CDS             5912..6712
                     /locus_tag="PLN77_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HRM42746.1"
                     /translation="MKLFISADIEGCAGVALTDETHKKESVYQRFAEEMTEEVVAACE
                     AAHEAGADEIVVKDGHGDASNIDPLKMPEYVTLIRGKSGHPYNMMYGIDDSYDGVLYL
                     GYHAPAGDPNFSISHTSTGNSLYIHLNGKVMSECMLNSYTAASHHVPVLFLSGDEEIC
                     RQAKELIPGITTVETKHGVGAATWCKAKGKVISEIREGVKKAVAGQKKECQVALPEHF
                     TYEVTYKDWKKAYTMSFYPGMQKVDTFTNKLETDRWMDIVTAHCFVVY"
     gene            6756..7847
                     /locus_tag="PLN77_00280"
     CDS             6756..7847
                     /locus_tag="PLN77_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM42747.1"
                     /translation="MDIEMFGKLSDAFGPSGFEEDVIRTIADYCKEFDVENDAMNNLY
                     VRMPGTEKDSRPVIQLDAHLDACGFMVQNIQDNGCLGIIMLGGFHLTSLPAHAVWIRT
                     RSGKMVHGIICAKPVHFMTTAERSSQTLEIEKMYVDVGATSREEVENVFGIHIGDPMM
                     PDVTFEYDAEHEICFGKAFDNRAGCACIVDTMKQLEKEQASLAVNVVGAFAAQEEVGT
                     RGAVVTSQIVKPDLAIVFEGSPSDDFFISAGQAQGCMKKGVQIRILDNSYISNTQFIS
                     LAEEVAEKCGIKYQESVRRGGSTNAAKISVTGKAVPCLVLGVPSRYAHSHYNFCAKED
                     LEAASAMAANVIRALDEEKIRHICHQDVL"
     gene            7944..8684
                     /locus_tag="PLN77_00285"
     CDS             7944..8684
                     /locus_tag="PLN77_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRM42748.1"
                     /translation="MRKPIIGILGNTYKTQPGLFSSAERMYVNSDYIESVLNNGGIPM
                     AIPAVAMKADPEGILAVCDGILVPGGEDLNPWYYGEEPKPQIQTIRPEIDEAWFALGR
                     AAKEMGMPMLGICKGIQFLNVLCGGDLYQDIYTQKETTILHLQSLERSYLHHHVEIKE
                     GTHLAEILGAGTHAVNSMHHQAVRTPGEDIVVSATAPDGTIEAIESRNGQIVAVQWHP
                     EGLIHSAPEMNRLFADLVERSSRYREKR"
     gene            8814..10124
                     /locus_tag="PLN77_00290"
     CDS             8814..10124
                     /locus_tag="PLN77_00290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRM42749.1"
                     /translation="MNRTIRVSAAGDILIMKRLLPGYQDVLPIREFLMQGEVRMANLE
                     TTISDGSCYASAYSGGTWLTADAKCLEDTLRYGFNFLGISNNHTMDYSYEGLASTISE
                     LKKKDVAYAGAGKNLYEASRPAILDTTAGQIAVIDICSTFENAARAGSQTERQPGRPG
                     LNPLRFSEKYTITEKHAQDLAKIAEVTGINGLRDLHRQEGFIAPLPEGVMEFGGKMFE
                     ISKDGTEGRSSSPNPIDVKRTVDAIREAKMTCEAVLVMVHSHEIRKDNDEEADYFLEE
                     FAHACIDAGASAVIGGGTHQLKGIELYHDCPIFYCLGNFIFENQYVRLLPADYMEKYG
                     LNIHTAASIGIARRQEQSSHSLYEIPEVYRSVLPYFEICQGKCTHLELLPVELGMDRE
                     NAEKNIPYVADEKTAEKIAEYLTRVSRRYGTEWEYKEGRIVLHA"
     gene            10117..11814
                     /locus_tag="PLN77_00295"
     CDS             10117..11814
                     /locus_tag="PLN77_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM42750.1"
                     /translation="MRKKWEIEEEYRNFCRNNKELALQTLRELTLTPTETGKEDQRIA
                     YCMEWMKQQGMESVHTDELGNVIWEYRPEQEKKVLYTAHLDTVFSLEEPLEIKEDGMI
                     WRCPGITDDTVNVVMLLMAAKYVHETEPELPCGLIFAADLGEEGLGNLCGVRALVGHY
                     EKNLCGMAAFDLYRDKMYPICIGSVRYRISAKTKGGHSFLNFGRKNAIAELAGLIGEL
                     YRFQTDAASHTTYNVGKIEGGTSVNTIAQDASMLFEFRSEDYRSLEACETYLEETIAA
                     RQNEEVQYSCELVGKRPCARETDPVQMARMTRCAQKTLKAADGEEPVCSEASTDCNIP
                     LSRHIPAICVGFCRGGGAHTREEWLDAASVEDGMCAAVALVCRLPWMCCESRVVVRDG
                     IEDPKEKEEIQQLLELCDQDFVPPLSHRNSTSQTNWAETEEKTDGIAEYLENICSQHV
                     VLWKEEGVVRAFMTWKDHFNCENLEAYPDSCYLTTLCVWPDYRGQGISEVMYAEAEKD
                     IAAKFPGSRITLRTWSTNGAQEHILDKLGYSLVRRLKDDRGEGIDTVYFVKKEENNR"
     gene            11887..13371
                     /locus_tag="PLN77_00300"
     CDS             11887..13371
                     /locus_tag="PLN77_00300"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRM42751.1"
                     /translation="MPCTTILVGKKASYDGSTMIARNDDSGAGHYTSKKFVVIHPEEQ
                     PRTYKTEISHLTIELPDNPMRYTAVPNAEKGEGVWAASGVNAAQVGMTATETITSNPR
                     VLGADPLVVYQPAEDGKEEVPGGIGEEDLVYIVLPYIKSAREGVKRLGSLLEQYGTYE
                     MNGIAFQDHDEIWWLETIGGHHWMAVKVPDDHYVAMPNQLGIDHFDLEDALGGQKEYM
                     CSADLKEFIETYHLDLSMDGNFNPRDAFGSHDDSDHVYNTPRAWFMERYLNPHTKKWD
                     GPYADYTPVSDDIPWMMVPEKKITVEDVKYVLSSHYQGTPYDPYAAYGDKSWKGAYRS
                     IGVNRTDFLSVIQMRPDHKGDNGILQWIAFASNAFNVLVPFYTDVTTTPEYLSNTTGE
                     VSTDNFYWASRMVAAMADASYKSSIFHIERYQEKVMAKGHALIHQYDALLAGETDETK
                     QTQLREEANNKIAEMLKKETGATLHKVLFELSNQMKNAYSRSDA"
     gene            13716..13865
                     /gene="rpmG"
                     /locus_tag="PLN77_00305"
     CDS             13716..13865
                     /gene="rpmG"
                     /locus_tag="PLN77_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRM42752.1"
                     /translation="MRVKITLACTECKQRNYNMTKEKKNHPERMETKKYCRFCKKHTM
                     HKETK"
     gene            13887..14087
                     /gene="secE"
                     /locus_tag="PLN77_00310"
     CDS             13887..14087
                     /gene="secE"
                     /locus_tag="PLN77_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRM42753.1"
                     /translation="MAETEKKASKKSWFKGLKAEFRKIIWVDNKTLGKQTVAVVAISA
                     VTCGLITLVDSAVIEFVNLLIK"
     gene            14104..14625
                     /gene="nusG"
                     /locus_tag="PLN77_00315"
     CDS             14104..14625
                     /gene="nusG"
                     /locus_tag="PLN77_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRM42754.1"
                     /translation="MSEVNWYVVHTYSGYENKVKANIEKTIENRHLEDQILEVRVPME
                     EVVEVKNGTKKQVLRKLFPGYVLINMIMNDDTWYVVRNTRGVTGFVGPGSKPVPLTDE
                     EMVNLGITGGEEKVELDLEVGDAVVVTGGAWKDTTGLIQSINDTKQMVTINVELFGRE
                     TPVEISFAEVKKM"
     gene            14698..15123
                     /gene="rplK"
                     /locus_tag="PLN77_00320"
     CDS             14698..15123
                     /gene="rplK"
                     /locus_tag="PLN77_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRM42755.1"
                     /translation="MAKKVTGYIKLQIPAGKATPAPPVGPALGQHGVNIVQFTKEFNA
                     RTADKGDLIIPVVITVYADRSFSFITKTPPAAVLLKKACNLKSGSGVPNKTKVATISK
                     DKVREIAEMKMPDLNAATVEAAMSMIAGTARSMGIVVED"
     gene            15203..15895
                     /gene="rplA"
                     /locus_tag="PLN77_00325"
     CDS             15203..15895
                     /gene="rplA"
                     /locus_tag="PLN77_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRM42756.1"
                     /translation="MKHGKKYLEAAKAVDRSIQYDPADAISTVKKLATAKFDETIEVH
                     IKTGCDGRHADQQIRGAVVLPHGTGKQVRVLVFAKGAKADEAVAAGAEFVGAEELIPR
                     IQNDGWFDYDVVVATPDMMGVVGRLGRVLGPKGLMPNPKAGTVTMDVTKAVKEIKAGK
                     IEYRLDKTNIIHVPIGKASFTEEQLSDNFQTLIGAINKAKPATLKGQYLKSVTLAPTM
                     GPGVKVNTLKLA"
     gene            16170..>16428
                     /locus_tag="PLN77_00330"
     CDS             16170..>16428
                     /locus_tag="PLN77_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42757.1"
                     /translation="MQYFEKQGNALIFRQDGETVRVEPWQKDSLRVRGTLLSEIEEGS
                     IALLDPEPVEAEIELVDELKATIKNGKIQAVQFPLPPVPDQA"
     assembly_gap    16429..16438
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16439..>17461
                     /locus_tag="PLN77_00335"
     CDS             <16439..>17461
                     /locus_tag="PLN77_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRM42758.1"
                     /translation="IQAVLELQPWGQKLRITFLNQKGEVLLSEIANGGALCLRAHDYR
                     ALKGGAYQLKVSFDSNPDEKIYGMGQYQQERMNLKGCNLELAHRNSQASIPFYVSSLG
                     YGFLWHNAAVGEVHFGTNTTEWLARTTKQLDYWVTAGDTPAEIEEHFADAIGKVPMMP
                     EYGLGFWQCKLRYYNQEQVLNVAREYKKRGIPLDVFVIDYYHWPRCGDYRFDEEYFPD
                     PKAMIDELHEMGIETMVSIWPQIDWRSENYEEMKQQGLLVKSNAGVDVQMLFHGNNVF
                     LDATNPRTRKYVWEKVKKNYADLGIRTFWLDEAEPEFSTYEYECYRYAAGPVEEIGNI
                     YPREYS"
     assembly_gap    17462..17513
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17514..18251
                     /locus_tag="PLN77_00340"
     CDS             <17514..18251
                     /locus_tag="PLN77_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRM42759.1"
                     /translation="GQEDIVNLVRCAWLGSQKYGALVWSGDIFSTYEDFRKQICAGLH
                     MGLCGIPWWTTDIGGFHGGVTEDPDFQELLVRWFQFGTFCPVMRIHGNRGPREEIINK
                     AGEVREGTGADNEVWSFGEKNYEILTKFIGVREKMRDYTRSLMAEAHEKGTPVMRTMF
                     YEFPEDAACWDISDAYMFGSDILVAPIVRAKATSRTVYLPAGASWTLANTGDVYEGGK
                     AYEIEAPIETLPIFLRDGKQGYLVGEI"
     gene            complement(18454..19374)
                     /locus_tag="PLN77_00345"
     CDS             complement(18454..19374)
                     /locus_tag="PLN77_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM42760.1"
                     /translation="MKETVLGSHLRKVPSIAVGCMRLSEKSKDEMNHFIHSALEQGAY
                     FFDHADIYGSGMSETVFGDAFASDPSLHREDVLIQSKCGIRQGCYDLSKEHILESVDG
                     ILKRLQTDYLDLLLLHRPDALVEPEEVAAAFDVLFESGKVRHFGVSNHKPMQIALLQK
                     YVRQPLVVNQVQFSIPVSNLVANGMEVNMETPGSVDHDGSLLDYCRLHDITLQAWSPF
                     QMPSWKGCFLGSDEYPELNQKIRVLAEKYGVSDTTIAAAWILRHPANMQLVTGTASEK
                     RLKEIIAACNITLTREEWYELYLAAGHPLP"
     gene            19659..20435
                     /locus_tag="PLN77_00350"
     CDS             19659..20435
                     /locus_tag="PLN77_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRM42761.1"
                     /translation="MDAKIIAGILAAGGAALAAGGVYRMNKKTGYFKKGNSVRYDVSR
                     IPFKKTSPLKGKTVVFLGSSVTKGFAAHNNAFAEYIAKKDSCICIKEAVNGTTLIDNC
                     EDSYIERMRDNLDPERQVDLFICQLSTNDATRNSPLGEISESRDLDSFDVKTVCGAIE
                     YIIAYAKETWHCRVMFYTNPQYDSDTYAKMVEALLKIQEKWQIGVIDFWNDERINSIS
                     PEKRKLYLQDPIHPMKAGYAEWLVPVMEQRIAEYLETQVS"
     gene            complement(20496..21188)
                     /locus_tag="PLN77_00355"
     CDS             complement(20496..21188)
                     /locus_tag="PLN77_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM42762.1"
                     /translation="MGKIDNNKQMKRESLLDSAFSLFIDNGFNKTSISDIVNNAGVAK
                     GTFYLYFKDKYDIRNHLIAHKASQIFQAAYADLLRHPDIQDFEEQVLFITDNILDQFA
                     ANHSLVTLISKHLSWGFFKNSLTFSPFSDAPAIYTIYESLLSHAAYTYRSPELMFYMI
                     LELVSGTSYNAILYQQPIPLEDLKPELYDAIRGIFKQFRIRKKRNVTESTEKTISSAQ
                     QPDHPAPLRAAQ"
     gene            21540..24638
                     /locus_tag="PLN77_00360"
     CDS             21540..24638
                     /locus_tag="PLN77_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42763.1"
                     /translation="MNRNRKLRKQMMSGLAVGMAAVMGAAPVYAATGEDAAVSKEETV
                     YVNANADGSEDQITVSNWLKNAGTEKNLQDESELKDIKNVKGDEEFTQSGNKLTWQTD
                     GEDIYYQGTTTKEPPVSVKFTYYLDGKEIKPDDLAGKSGKLKIKVDYTNNTKTTVDIN
                     GKKETMYTPFVLMTGMILPQDTFSNVKIDNGKVISDGSRNIVIGFGMPGLQESLGLNS
                     DSLEDLVSGDSDFELPESLEITADVENFSLDSTFTVALDDVLDTIDTDNITGYDDLKD
                     ALSDLEDAAMELVDGSKALSDGVDTLKDSYKELDDGIGTLKGGADELNSGAGTLAGGA
                     GTLASGASTLANGTSTLNSGASTLNSGAATLSNGASTLSSGASSLASGVNSYTEGADT
                     LNKAIQTYLGKNGVLTGSVTEYVNGVNKVVAGVKQYTDGTTQLSDGVASYVAGEKQLA
                     AGAEKLQPLADGLTEVNSAIATLNAALDGKDENDILAGAKQLSEGLTSLNEQLQSTEV
                     QSLLTMVDGMTSTGSTLISETEALSEVLNTTIAGSVNSMMTNGNELMSQMDTLSKGLE
                     SYLSGLEAQITNQATQMGTQMAAQVQAWADGAAAQLNQEATAAAQSAADAAASQASAD
                     AAKQANDQLSAAAATLDAQISAAKSAGNTELADSLSKARNQIGSVSVSASAGTVSVTP
                     VTAPQITLPELNFEIERPDLSGLAAMQQKMSDIKSQYSDLQKTVAALMPQLETMQKQM
                     QTITDNKAVVEQNLGAMQKLTAGVAQLDAGGSALYKGIGTLSAGVSQLYSSTNESFAK
                     ATAGINALNAGFTQLSAYNDTLLSGASQLKANAPALVEGVTTLQGGTTQLTAGLGQLG
                     SQLSDGSAKLSANSGSLRSGASQVASGASQLASGASTLANGTSSLASGASELNSGAST
                     LASGASTLASGASTLANGTSSLASGASDLKSGSSKVVDGIDELSDGAKELADGTKKFE
                     EEGTSKLKSTFEDDLGDVLDRIQALNSDKCAYTSYSGKANGMDGSVKFIIETAAIEAA
                     EE"
     gene            complement(25174..26106)
                     /locus_tag="PLN77_00365"
     CDS             complement(25174..26106)
                     /locus_tag="PLN77_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM42764.1"
                     /translation="MISVLFPDAACFTYDLCGKFQSPSPEWMHLTRNLVNFELMVVTE
                     GTLFIADERRSYEVHPGEYLLLPPTVHQHGSKKGSCAFYWLHFIPGIPSAPIIAPSDP
                     TAGSVLDEFHAAPPSSYRLEDLAPAQLPSNYPETLCIPRQGTLSSPNRVIILMKQLQD
                     TSLRFHNTFLNNSLTAAVLGEINCQSPFYRTYTSETQRTQILQDIISYIQYHSSESLK
                     VTELARYFGYNDKYLSSLFHEGTGMTLKQFILKSKMDSAMAELTDTNHTVSQIAYNVG
                     FQDAHNFSTAFRKLTGMTPGQYRKSYGNRKLSFE"
     gene            26296..28305
                     /locus_tag="PLN77_00370"
     CDS             26296..28305
                     /locus_tag="PLN77_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HRM42765.1"
                     /translation="MDQKNFSKPLSLAKVQVTDAFWKKEMELVRTEVIPYQWNALNDN
                     VPGAAPSFCMRNYRRAGEVEKERKAKGDKFVQIKYPLDTFETLPKDGKMDGRFYGFLF
                     QDTDFTKWVEAVAYSLTQHPDPELEKTADEAIEAVCAAQREDGYLDTYYLINDQDMIF
                     TNLKDNHELYCFGHLTEGAVAYYQATGKDHLLKAAERFADFIASKLGHGEGKKAGYPG
                     HEIAEMALMRLYDLTGEQKYLDLAKYFIDERGTKPYYYDIERGLTCPEGEERYSYNQA
                     NRPVRQQDEVQGHSVRAVYLYSGMADVARATQDESLLKACETLWNNMVHQKMYVTGGI
                     GATHIGEAFSFNYDLPNDTAYAETCASIGLVFFARRMLEIQAKAEYADVMELALYNGV
                     LSGMALDGKSFFYVNPLEVLPEACHKDERKFHVKPIRQKWFGCACCPPNLARTVSSVA
                     SYAYTENDTTLFVHLYMGGTVEGEKVKASITSEFPWDGHVSVTCESDTKEPYTFAFRI
                     PGWCASYEKEIPKGAEVEEKDGYLYVTKVWAKGETIRLNFPMEIQFLASNPLVREDAG
                     RVAVKRGPVVYCMEEADNGKNLHLTKLCVNGEKTAEAADELGEHFVRVTAEGRRMKLG
                     DAEKQPLYHLYQKEEEEKTKLKLIPYYMWNNRGEGEMQVWIRACE"
     gene            complement(28458..29069)
                     /locus_tag="PLN77_00375"
     CDS             complement(28458..29069)
                     /locus_tag="PLN77_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM42766.1"
                     /translation="MMNTNRTLKLTTLALCGVINILGGTIALLLRLPVYLDSIGTVLA
                     AALFGPAAGLVPGLISGLISGMTSDVYAFYYIPVQLVIGLVAGLVFRRLRPSHTAGSR
                     SESLSLRSMGWKLFPAALVISLPGTVVSSTITAVVFGGITSSGSTVLVQLLHSLGMNL
                     TLSVCVVQALTDFVDRMIVLAVVLVILPAVSSAFGSLSMRSRK"
     gene            complement(29066..30112)
                     /locus_tag="PLN77_00380"
     CDS             complement(29066..30112)
                     /locus_tag="PLN77_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HRM42767.1"
                     /translation="MNAKKLIIDCDPGIDDSLALMLAAASKELDLVGITTVSGNVPAS
                     MGAKNALKILSMIGKPDVPVYVGEETPLVRDYVDAMDTHGMDGLGESGWPDAEGLTPN
                     KDAVSFLIETLKATPDVTILALGPMTNLAKVISRAPECLTNLTALVSMGGSYKSHGNC
                     SPVAEYNYWCDPDAAAEVFRAFSELPQLAGKKLHMIGLDVTRKIVLTPNLVEYMKCVN
                     PGVGTRIEELTRFYFDFHWKQEKVIGCVINDPLAAAYLVDPSLCSGFDAYTQVETGGL
                     CIGQTVVDAMNFYQKAPNSHILTQVDVRRFFLMFFERVLGVPQEESAPVLDQILTAPP
                     VFPHETMFQKGGEA"
     regulatory      complement(30141..30185)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30346..30813
                     /locus_tag="PLN77_00385"
     CDS             30346..30813
                     /locus_tag="PLN77_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42768.1"
                     /translation="MADGFLAPTGKFYPKTENFHAQTARAILGPDGQTDEPIQELLRR
                     GYILFVGFHKPGEPENLHADMDYVLGGPGYPATEGQKAWIAEHTEELSRKQQFDINND
                     ETNFENFYISNVRMFPWCKGCAEEKARDLWGNAQSEEKPKRCDACPAFRNRPL"
     gene            31102..31518
                     /gene="rpsL"
                     /locus_tag="PLN77_00390"
     CDS             31102..31518
                     /gene="rpsL"
                     /locus_tag="PLN77_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRM42769.1"
                     /translation="MPTFNQLVRKGRETSVKKSTAPALQKSYNSLRKKSTDMSSPQKR
                     GVCTAVKTATPKKPNSALRKIARVRLSNGIEVTSYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGTRYHIIRGTLDTAGVANRKQARSKYGAKRPKKK"
     gene            31692..32162
                     /gene="rpsG"
                     /locus_tag="PLN77_00395"
     CDS             31692..32162
                     /gene="rpsG"
                     /locus_tag="PLN77_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRM42770.1"
                     /translation="MPRKGHTQKRDVLADPLYNNKVVTKLINNIMLDGKRGVAQKIVY
                     GAFAKVEEKAGKPAIEVFEEAMNNIMPVLEVKARRIGGATYQVPIEVRPDRRQALGLR
                     WLTAYSRKRGERTMEDRLANEILDASNNTGASVKKKEEVHKMAEANKAFAHYRF"
     gene            32178..34295
                     /gene="fusA"
                     /locus_tag="PLN77_00400"
     CDS             32178..34295
                     /gene="fusA"
                     /locus_tag="PLN77_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRM42771.1"
                     /translation="MAGREYPLERTRNIGIMAHIDAGKTTLTERILYYTGVNYKIGDT
                     HEGTATMDWMEQEQERGITITSAATTCHWTLEENCKPKPGALEHRINIIDTPGHVDFT
                     VEVERSLRVLDGAVGVFCAKGGVEPQSENVWRQADTYNVPRMAFINKMDILGADFYNA
                     VDQIKTRLGKNAICLQLPIGKEDEFKGIIDLFEMKAYIYNDDKGDDISIVDIPEDMKD
                     DAELYHTELVEKICELDDELMMRYLDDDIPSVDEMKAVLRKATCECTAVPVCCGSAYR
                     NKGVQKLLDAILEYMPAPTDIPPIEGTDLDGNPIVRHSSDDEPFSALAFKIMTDPFVG
                     KLAYFRVYSGTMNSGSYVLNATKDKKERVGRILQMHANKRMELDKVYSGDIAAAIGFK
                     FTTTGDTICDEQHPVILESMEFPEPVIELAIEPKTKAGQGKLGESLAKLAEEDPTFRA
                     HTNQETGQTIIAGMGELHLEIIVDRLLREFKVEANVGAPQVAYKETITKPADIDSKYA
                     KQSGGRGQYGHCKVKFEPMDANGEEIYKFESTVVGGAIPKEYIPAVGEGIEEAMKAGI
                     LGGFPVVGVHANVYDGSYHEVDSSEMAFHIAGSLAFKEAMQKGAPVLLEPIMKVEVST
                     PEDYMGDVIGDINSRRGRIEGMDDIGGGKMIRGFVPLAEMFGYATDLRSKTQGRGNYS
                     MFFDKYEQVPKNVQEKVLSAKAK"
     gene            34493..35686
                     /gene="tuf"
                     /locus_tag="PLN77_00405"
     CDS             34493..35686
                     /gene="tuf"
                     /locus_tag="PLN77_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRM42772.1"
                     /translation="MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKVLSTRVEGNT
                     AENFEDIDKAPEERERGITISTAHVEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGVMAQTKEHILLSRQVGVPYIVVFMNKCDMVDDEELLELVDMEIRELLN
                     EYDFPGDDTPIIQGSALKALEDPAGPWGDKIMELMDAVDSWIPDPQRDTDKDFIMPVE
                     DVFTITGRGTVATGRVEAGVLHLNEEVEIAGLKEETKKTVVTGIEMFRKLLDEAQAGD
                     NIGALLRGVQRTEIERGQVLAKPGTVHCHTKFTAQVYVLTKDEGGRHTPFFNNYRPQF
                     YFRTTDVTGVITLPEGTEMCMPGDNVEMTIELIHPIAMDQGLTFAIREGGRTVGSGRV
                     ATLID"
     gene            36021..36686
                     /locus_tag="PLN77_00410"
     CDS             36021..36686
                     /locus_tag="PLN77_00410"
                     /inference="COORDINATES: protein motif:HMM:NF021201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2399 domain-containing protein"
                     /protein_id="HRM42773.1"
                     /translation="MKPLIYQYRMQWRELLQCVGVVPDNISSMVHAFGIRLKKQEIWH
                     PAYEAFCRCGEPYVLTMENLKGITEVQPVGTCVYIVENEMVFSYLMEQVQGKNVSLLC
                     TSGQPRYAALKLISLIVQSGILIYYSGDLDPDGIGIADRLWQRFGNRIQFFGMSPEDY
                     RNSLSKEVFGENGRKKLEHIWHPLLRETAELVRKTGKAGYQENILKELSEKLVGCDQN
                     QNL"
     gene            36739..40134
                     /locus_tag="PLN77_00415"
     CDS             36739..40134
                     /locus_tag="PLN77_00415"
                     /inference="COORDINATES: protein motif:HMM:NF020044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HRM42774.1"
                     /translation="MITKADIKQETNSVSYNRGKKIYEEQKVHAFQVQEMEDIFGYQL
                     HKITAVVDGSGKNMYCVSVSVDEEMSEIMEDDCDCPAHEQYWGLCKHCVAVLLYYLEW
                     RKKERKKLEEKVRNDEEHQELEQLLRAVGVKKGMEEFQGEHKIVRKVVKPTPKAETSP
                     GFSELLSHYVMRDQVAFLPKAKAGQVKLEIHLESQFDDSFRAEFKIGSKRMYVLKSVS
                     KMTAAVKNMETVSYGAQLSFLHCMDAFEEESRPMVEFLSAYTMERGSCYQIGTGSYAS
                     CFDDRYVMISVQNMDEFFEAVGSQEFFCRTNWREEKLWHCQEGMPQYEMKLLRKADGL
                     KVQMYADPIFYGRKYLYFLSEKNTIYRIPREEIAEVCEFLEYMERCPDGVCEIAKEDI
                     PTFCQGLLPVLEEHFKVKKEEKLELQQYLPPQVEFQIYLDAPQHDMITCELLAVYGEK
                     KYNVFADANDIHQLSHGRDVRKEAAANQLVRSCFSAYDARKHQMLLQGADEMYEFLSS
                     GMEDLQKLGEIFVSDRLKAIRVIPSPKVSVGVSLAENVLELHLNPGNFGMEELAEILS
                     KYDRKKKFYRLRTGEFMDMDEDGIRVLSELRENLQISEAKLKSGEITIPKYRALYLDT
                     RLKEQDDLQVEKNREFRMLIRNMKTAEENDFELPDNLQAQLREYQKTGFWWLKTLCQN
                     GFGGILADDMGLGKTLQTIAFLLSEMQEAPEDANRRSLIVAPASLVYNWESECARFAP
                     ELQTRLVAGTQEQRKEMIQNAGMQDILITSYDLLRRDIELYQDLPFFCEIIDEAQFIK
                     NHATQGAKAVKTIHASFRLALTGTPVENQLSELWSIFDYLMPGFLYGYQKFREQFELP
                     IVRNGDEERLERLQKMIRPFILRRLKKEVLKDLPDKIEKNMAACMEQKQKELYHAHAQ
                     RLALMLQNQTEEEFADSRFQVLSELTRLRQLCCDPALVYENYEGGSAKLDLCIELIQS
                     AVESGHKILLFSQFTSMIEILTARLQEESISCFVLQGSTKKEQRAQMVEQFNRDDTSV
                     FCISLKAGGTGLNLTGADIVIHYDPWWNVAVENQATDRAHRIGQKNVVTVYKLIAKGT
                     IEEKIIKLQQMKKELAEEILSGDEIKTASFQREELLELLEERF"
     gene            complement(40285..41298)
                     /gene="asnA"
                     /locus_tag="PLN77_00420"
     CDS             complement(40285..41298)
                     /gene="asnA"
                     /locus_tag="PLN77_00420"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRM42775.1"
                     /translation="MGEIIIPQGYKSALNLHDTQVAIKTVKDYFQHELTHRLHLLRVT
                     APLFVQPETGLNDNLNGVEHPVSFELKDQGTKKTAEIVQSLAKWKRYALHKYGFSEGE
                     GIYTDMNAIRQDEVTDNIHSVYVDQWDWEKIMSPEERNLDFLKSTVKDIYEALKNTEK
                     HMADQYDYIETILPDEIFFITSQELEDRYPNLTPKEREYTITKEKGAVFIMQIGGVLA
                     SGERQDGRSPDYDDWSLNGDLIVYYPVLDIALELSSMGIRVNADALRAQLKECGCQSR
                     AKLPFQKSILNGTLPQTIGGGIGQSRICMFFLRKAHIGEVQCSVWPMETREVMDVMNI
                     QLL"
     gene            complement(41513..42364)
                     /locus_tag="PLN77_00425"
     CDS             complement(41513..42364)
                     /locus_tag="PLN77_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRM42776.1"
                     /translation="MRKLSVFYEHILQACEQSGKTIPEVLAFCREQGISAVEMNYSLF
                     ASEREVIAPMLSDAGLVISCMHETFDFSHDTDLGRAQQMLDTAASQQVSRVLFVAGAL
                     ETAEAAELAACSSTYEATSTFMAENKSIQNMVHALSSLAEYAALRGVTITLEDFDGFL
                     QPFARTYPLLWFMEHVPGLRYTLDIGNFAFSDEDVSYAAWLLKDYIVHVHCKDRAESP
                     LVHGRFCRGLGQTPAGTGYLPMAKLLTDLEACDYDGYLAIEHFDAPDQLSFIEQSAAF
                     LRQWVSE"
     gene            complement(42498..43241)
                     /locus_tag="PLN77_00430"
     CDS             complement(42498..43241)
                     /locus_tag="PLN77_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HRM42777.1"
                     /translation="MIRVTVWNEFQHERTQEKVKEIYPDGIHTCIAKFLGQEEELSVR
                     TAVFDEKEHGLTEEVLANTDVLVFWNHMLQEEFSDDVAERVRRHVLAGMGLVVLHSGH
                     YSKIMRKLLGTSMTLRWRHGDRERLFCTCPTHPIAQGIPAWVDIPEEEMYGEFFDIPK
                     PDDVVFTGWFAGGEVFRSGCTFTRGLGKIFYFQPGHEEYPIYHMPVIQKIIKNGVRWC
                     VPVVRRPAPLDNAWVNPSTEEVYLSSHQK"
     gene            43456..43718
                     /gene="ffs"
                     /locus_tag="PLN77_00435"
     ncRNA           43456..43718
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLN77_00435"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            43913..44992
                     /locus_tag="PLN77_00440"
     CDS             43913..44992
                     /locus_tag="PLN77_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRM42778.1"
                     /translation="MLRIGLLTSGGDCQALNATMRGVVKGLANNVKDLEVYGFMNGYK
                     GLIYSDYRMLSAKDFSGILTQGGTILGTSRQPFKLMRTPDANGLDKVEAMKHTYHKLL
                     LDCLVILGGNGTQKTANLLREEGLNIIHLPKTIDNDIYGTDMTFGFYSAVNIASDAID
                     CIHTTASSHQRVFIVEVMGHKVGWLTLYAGIASGADVILIPEIPYDIEKVCEAINRRK
                     ERGSGFTILAVAEGAIAKEDAALPKKELKKRQEEIAKKYPSVSYKIAEEIEAKTGMEI
                     RVTVPGHMQRGGSPCPYDRVLSTRLGSEAAQLILDKQYGYMVGMVNGKVKKIPLGECA
                     GKLKTVDPNAQEVLQAKRMGISFGD"
     gene            45136..46785
                     /gene="dnaX"
                     /locus_tag="PLN77_00445"
     CDS             45136..46785
                     /gene="dnaX"
                     /locus_tag="PLN77_00445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRM42779.1"
                     /translation="MSYTALYRKFRPSTFEDVKGQDHIVTTLKNQIRADRIGHAYLFC
                     GTRGTGKTTIAKILARSVNCEHPVDGSPCNECPTCKAILAGVSTNVIEIDAASNNGVD
                     NIREIREEVAYRPTQGRYKVYIIDEVHMLSTGAFNALLKTLEEPPSYVIFILATTEAN
                     KIPVTILSRCQRYDFRRITIDTIAARLSELMEKEGVDVEEKAIRYVAKAGDGSMRDAL
                     SLLDQCIAFYLGQKLTYDKVLEVLGTVDTEIFSQMLRTILAGDVTGSIRTLETLLNRG
                     KELGQFVTDFTWYLRNLLLVKSADETEELLDVSTENLKLLKEESTMVDDETLIHYIRV
                     LSELSGQLRYAAQKRVLVEVALVKLCKPQMEQNLESVLERLRILEEKMERGIPMMVTN
                     APGAGNGSAGGSGAAGDGYAAAETPQSAPAPVKAAPADLQRISASWKAIVGQTSGMFR
                     QFLQSAVPKYNSETGDAKLFVEFADQLASIYVERPEAADEIRTIIEQMTGKTVEVQLI
                     LADQHANAALSTISVDDALTQAIHGIPVEEDDESDFDDGEE"
     gene            46818..47171
                     /locus_tag="PLN77_00450"
     CDS             46818..47171
                     /locus_tag="PLN77_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRM42780.1"
                     /translation="MAKRGGFPGGMPGNMNNLMKQAQKMQKQMEENQKALEEKEFTAT
                     AGGGAVEVTISGKKEVTKVKISEDAVDPDDVEMLEDLIMAATNEALRKVEDEAAKAMG
                     KLAGGLGSMGGGFPF"
     gene            47171..47767
                     /gene="recR"
                     /locus_tag="PLN77_00455"
     CDS             47171..47767
                     /gene="recR"
                     /locus_tag="PLN77_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRM42781.1"
                     /translation="MDYYSGYINQLIEQLSHLPGIGVKTAQRLAFHIMNMPADQAKRL
                     ADSITEAKSHVQYCKCCQTLTDKELCPICANVKRDHSVIMVVETTQDLAAYEKTGKYD
                     GVYHVLHGAISPMLGIGPDDIRLKELMQRLQGDVSEVIVATNSSLEGETTAMYISKLI
                     KPTGIKVTRIASGVPVGGDLENIDEVTLLRALEGRTEL"
     gene            47770..48873
                     /locus_tag="PLN77_00460"
     CDS             47770..48873
                     /locus_tag="PLN77_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM42782.1"
                     /translation="MGRKKVTSSDVAAASGVSQATVSMILNRRENVSFSRETVEKVEA
                     AAKELGYQIPRRKMRRESNGNKLIVVFCPTLTNPYYVMLLHGIEEVAKEKNYGVFVCN
                     TQRDLKIEENYLRMMKSVRPRGIIYTCNPSRSCKEKVEELAAEIPLVIINNREELLEI
                     DAVELNNAKPGRLMARHLLELGHRHVAFVSPPLTSKQGQRLKRVEGFVREFEEAGYGD
                     GVIVKSADEDLDEVIPSMDSEYKMGYYLTKELLREDKNLTAIVGMNDMIAFGIMDAVL
                     ESKLRIPADISVVGCDNTVYSSFRSISLTTIDHYVPLKGRDACDIILRKIQSLRESQD
                     GNEPLSTYHVEYEPKLIVRRSTSYARTEKLKNK"
     gene            49154..49807
                     /gene="fsa"
                     /locus_tag="PLN77_00465"
     CDS             49154..49807
                     /gene="fsa"
                     /locus_tag="PLN77_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRM42783.1"
                     /translation="MRFFIDTAKVEDIKKANDMGVICGVTTNPSLIAKEGRDFKEVIK
                     EITEIVDGPISGEVKATTTDAEGMIKEGREIAAIHPNMVVKIPMTVEGLKAVKVLSAE
                     GIKTNVTLIFSANQALLAARAGATYVSPFLGRLDDISTRGVDLIREIVEIFDVAGIDT
                     EIIAASVRNPIHVTDCALAGADIATVPYNVIVQMTKHPLTDAGIAKFQADYKAVFGE"
     gene            49917..>50262
                     /locus_tag="PLN77_00470"
     CDS             49917..>50262
                     /locus_tag="PLN77_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRM42784.1"
                     /translation="MNKLELQKTANEIRKGIVSGVHAAKAGHPGGSLSAADIFTYLYF
                     EEMNVDPKNPKDPDRDRFVLSKGHTAPGLYAALAEKGYFPKEDLLTLRHLGSYLQGHP
                     DMKHIPGVDMSSG"
BASE COUNT        14519 a        11144 c        13324 g        11213 t
ORIGIN      
        1 tataccattt ttgacgaaaa taaacaacct aaatgcaaaa agacaaaaaa gaaaagaaaa
       61 aatcaggaag aatgctttga catagtgacg cattacatct ataataccga ttaagcaaag
      121 gggctgtaaa aaaaggtaag gcatctttgc tcttttttat atcatggttc tgttatctgt
      181 gaaaagaaag aacaaagaaa acagcaacac ttgcagaaaa ggaggagaca acaatgaaaa
      241 aaagacatgt aatgcgtgct ctggttgcag cagcaacagc actggcactg acagcatgcg
      301 gatcgacagg ctcttctgac acaagtaaga gcacatcaac agcatctgct gcatcgacct
      361 catccggttc ggaagcagat acggagaata ccatcacgat cccggcaggc gagtcggtat
      421 cccaggattc tgatgttacg gcaatggtag ctgtagactt taccaccatg gatccgatgg
      481 atacttccga tacactttcc ggtggtgtac agcgaatgat gatggacggt ctgtttggat
      541 tcgatgagaa tatgcagatc attccgatgc tggcaaccag ctacgaagca aacgatgaag
      601 caaccgagta tacgatccat cttcgtgagg gcgtaagctt taccgatgga actccgtgga
      661 atgcagatgc cctgattgca aacgtaaata aatgggcaga caagagcctt ggactgaaac
      721 gtactacatt cctctgcaat gttctggatc atgcagagaa gatagatgat tataccgtta
      781 aaatttattt atcacagtca ttcggtgctt tcatcagcaa cctggcacat ccggctacgc
      841 tgatcatgag cccgaaacag attgaagcag gagaagatgc ctgtgcacag gcaccggtag
      901 gaaccggcca gtacaaattt gttgagtggg ttgccggtga ccatatgaag gtagagctga
      961 acaaagactg gtggggatat gacgcagacg tctgcggcgg aactgctctg gcagatgcag
     1021 atgcaggatt taagagcatc acattcaaac cggttgcaga aagtgctacc cgtgtatctg
     1081 caattcaggc aggtgacgca cagattatgt ggtctgtacc gaccgaaagc gttgacacct
     1141 taaaaggtga cagcaacgta tccgttggaa tgggcgacag catcgccgta tggtacttct
     1201 tcatgaatac acagaaagca ccgttcaacg atgttaaggt tcgtgaggca atggcttatg
     1261 caatcaacaa agaggcatat atccaggtcg ttatgaacgg ttacggttct gtggcaactt
     1321 ctatggtagg agaagaagta cagcactaca aaggaaatga tccgtattcc tacgatccgg
     1381 aaaaagcaaa agagctgtta aaggaagcag gatatccgga tggatttacc acaacactga
     1441 tgtacagcaa cacgacagca aatcagaaaa aagcagagtt ctacaaacag cagctttctg
     1501 aggttggtat tgatctggaa ttgaacggaa tggaaaatgc cgttctgaat gaaaaggttc
     1561 agggagctga ctgcgcagga gcagatgccg aggttgactg ctacttaagc ggatggtcta
     1621 cttccaccgg tgatgctgac tggggacttc gtccgatgct ggcaaccgag tctgagccgc
     1681 cgatgagcta caatgtcagc tactatgaga atgaaaaagt agatcagctt ctcaaagacg
     1741 gtcttgcaac tgcagatgaa gacaaacgtg gtgagatcta tggagaagta caggatatcg
     1801 tatggaagga tctcccgctg ctctgcatcg caagcgataa aaacatctgg gcaaccagca
     1861 acaacattac cggagtatat ctgttaggcg atggaagcat cggcatgaga aatgccagaa
     1921 tggcagccga ataacggcag actgaaacaa ggatatgaaa aagaccccgg cgtccttcca
     1981 tcaaaagagg gcgccggttt cttacaaaca ggaaagcagg cagtgatccg tgggattgta
     2041 cctgtaggat aagcatgaaa gtaggagata caatatgcca aaatatatac tgaagcgtat
     2101 tgtgggtacg atccccacac tgattattgt tgttacattt gtatttttct ttgtccgcct
     2161 gattccggga gatccggcga gactcgttgc aggagagcag gcaacacagg atgacgtaga
     2221 ggcagtaaga gaagagcttg gcctcaataa atccattccg gcacagtatg ttacctacgt
     2281 gacaggactt ttaaaaggaa atctcggaac ctcccttcgc acaaagcagc cagttacaaa
     2341 ggaggttgcg ggaagattcg gaaataccgt tcgtctggca tttgtcacat tgctgtggag
     2401 ttcggttgtg ggtgtgctct ttggtgtgtg gtccggaact cacaggggaa aatggcagga
     2461 ttataccgga atgacgattg cggtctcagg tatttcgctc ccctcgttct ggatcggctt
     2521 tctgctgatt atgctgtttg cggtaaaact gcgaattctg ccgacaacaa gcggaacgag
     2581 cctgaaaagt ctgattatgc cgtctattac gcttggcgtc ggaattgcag ccgtgatcgc
     2641 acgatttaca agatcctcca ttattgaggt tttaaaagaa gattacgtca gaacagcaag
     2701 agcaaaagga atcaaagaaa aggttgtgat ctggaagcat gttttccgca actccatgat
     2761 ctccgtcgtg acggttgtgg gtctgcagtt cggctttctg ctgggaggtt ctgttgtcac
     2821 agaaacggta tttgcctatc ctggccttgg ctcccttctg attgaatcgg tcaattacag
     2881 agattatccg gcaatccagt ccctgattct gatcttttcg ctgcagttta tcgtcatcaa
     2941 cctgattgtc gatatcctct atgcggttct gaatccggaa attaaacttc agtaggagat
     3001 gagtggaatg aaagaaaaga aaaaagtaca gaccgagaac caggagagaa ttaaaactcc
     3061 gttttctgag atggttcgaa aatttaaaaa acagaaaaat gcagtagttg cgctttgttt
     3121 tatcattttt ctcattttcc ttgcgttttt cggaagcaaa attgtgccgt acggaatcaa
     3181 tgagtatgat tacaatgcga ttctgcaggg accaagtatg aagcacctgt ttggaacgga
     3241 tgagtttgga cgggatctgt tttcccgtat cgtctgtgga acccggattt cacttgcagt
     3301 cggattcggc gctgttacgg tcggtgcagt ctgtggtacc attctcggac ttctggccgg
     3361 atattacggc ggatggattg attctattat tatgagaatc tgcgatgtcc tgttcgcttt
     3421 tccgggactg attcttgcaa tcgccatcgt ggcaatcctc ggaagcggca tgacaaacgt
     3481 tgtgattgcg gtcgctgtct tttctacacc gaccttcgca cgacttgtcc gaagcaaaac
     3541 gctgaccctg aaaaattccg tttttgtcct ggcggcacgg aacatcggtg tttccgatgc
     3601 gagaattctg tttcaccata ttctgccggg agctattccg gacatcattg tacagtattc
     3661 catgtcattt ggatcttcca ttctgacggc atcgagcctt tccttcctgg gaatgggagc
     3721 acagccgccg acaccggaat ggggactgct tctttccaac ggaagaaact atatgacgag
     3781 cgcaatgtat gtgacgattt tcccgggtct tgcgattttc cttaccgtac tgagctttaa
     3841 cctgttaggc gacggacttc gcgatgcact cgatccgaaa ctttccgatt gataaggagg
     3901 agcagaagtg gctactgata acgtacttga gataaaaaat ttacatacgt atttctatac
     3961 cgatggcggt gtgatcaaag cagtagacgg tgtcgatgtg gagcttcgac gcggcgcaac
     4021 gcttggcatc gtaggcgagt cgggaagtgg aaagagcgtg acatccctct ctgtgatggg
     4081 attactgacc ggaacgaaag gaaaaattgc tgatggcgaa atcctgctgg atggcgatga
     4141 cattgtaaag ctgacgaagg aacagcagag aaagctgcgc ggaagcaaaa tttccatgat
     4201 tttccaggaa ccgatgacca gtctgaaccc ggtcatgaag attggagacc aggtggcaga
     4261 atgcgttctg cagcacgaaa aaatatcaaa aaaagaggca ctgaaaaagg cggaagatat
     4321 gctccggaaa accggtgttc cgcgtgtgga acatatgatg aaggagtatc cgttccagct
     4381 ttccggcggc cagagacaga gggtcatgat tgccatggca cttgtatgca agccggaaat
     4441 cctgattgcg gatgagccga cgactgcact ggatgtaacg attcaggcac agatactgga
     4501 tctgatgaat cagctgaaaa aagagacagg tacatccatc ctgtttatta cacatgatct
     4561 tggcgtcgtt gcagaggtct gcgatgatgt tgccgttatg tactgcggcc gcgtggtaga
     4621 gcgcggagat gtaaaaacaa ttttcgccaa tccgtcccac ccgtatacga agggactgct
     4681 gggatcgatt ccgagactgg gagacgccgg aaaagaactg aagtcgattc cgggtaacgt
     4741 tccaaacccg aaatatatgc cgaaaggctg caaatttgag ccgcgatgtc catacgcttc
     4801 ggaaaaatgc cgcgaggaag aaccgggatt tttccagatc gaggaaggcc atatcagcag
     4861 atgctggctc tgcgaaagcg gacagctcaa aacagatcac gtagagaagg caggtgacta
     4921 ggatgtcgga tacattactt agagtagagg atttgaaaat ttattatccg gttgccggaa
     4981 gcggatttgg aaaaaaggaa ttcgtcaaag cggtagacgg agttaccttt gaggtgaaaa
     5041 aaggcgaggt attcggaatt gtaggcgagt ccggctgcgg aaaatccaca ctgggaagag
     5101 gggtctgcaa actggaaaat ctgacatcgg gccatgtgta tctggatgga gaagatatta
     5161 cagagtacaa tgaccggaga atgcgttcca tccggaaaaa ggtacagatg gtttttcagg
     5221 atccgtatgc gtccctgaat ccacggatgt ccgtttttga catcattgca gagccgctgc
     5281 tggtgcatca tctgtatcag gataaagcgg atctggaaaa gaaagtgctg gatcttttgc
     5341 accgggtagg actggatgac taccatgcaa accgttatcc acatgaattt tccggcggtc
     5401 agcgtcagag aatcggaatt gcccgtgcac ttgcggtaga accgtctctg atcatcgcag
     5461 acgaaccggt ttcggcactg gatgtgtcta ttcaggcgca ggttctgaat cttttaaatg
     5521 aactgaagca tgacctggat ctgacctata tttttgttgc acatgacctg agcgttgtgg
     5581 aatacatcag tgaccgtgtc ggcgttatgt atctcggcaa tttcgtggag gtcggagaaa
     5641 aagagaaaat ttacagcaat ccgatgcatc cgtatacgca ggcactgctt tccgcagttc
     5701 cggttccgga tccgacggca aaaagagaaa gaattctgct tgagggaagc atcccatccg
     5761 cccacaagcc gccgacaggc tgcaaattcc ataccagatg tccaaaatgc atggaatgct
     5821 gcaaaacaca ggcgccggag cggtatgaag tggatgacgg acattacgta tattgtcatc
     5881 tttatgataa agaaagaagg aaacagcaga aatgaagctt tttatcagtg cagatatcga
     5941 gggctgtgcc ggagtggcac tgaccgatga aacacataag aaggaaagcg tataccagag
     6001 atttgcagag gagatgaccg aagaggtcgt tgcggcatgc gaagcagcac atgaagcggg
     6061 tgcggatgag attgtcgtaa aggacggtca cggagatgca tccaacattg acccgctgaa
     6121 aatgccggag tatgtcaccc tgatccgggg aaagagtggt catccgtata atatgatgta
     6181 tggtatcgac gacagctatg acggtgtact gtatcttgga taccatgcac cggcaggaga
     6241 tccgaatttt tcgatcagcc acacatcgac aggaaacagc ctgtatattc atctgaatgg
     6301 aaaagtgatg agtgagtgca tgctgaacag ctatacagca gcatcacatc acgtaccggt
     6361 gctgtttctt tccggagatg aagagatctg ccgtcaggcc aaagaactga ttccgggcat
     6421 tacgacagtg gaaacgaagc atggtgtcgg tgcggcaacg tggtgcaagg cgaaaggaaa
     6481 agtgatatcc gaaatccggg aaggggtaaa aaaggcagtt gccggacaga aaaaagagtg
     6541 tcaggtggct cttccggagc actttacata tgaggtaacc tataaagact ggaaaaaagc
     6601 gtacacgatg tcgttttatc cgggaatgca gaaggtagat accttcacaa ataagctgga
     6661 gacagatcgc tggatggata ttgtcacagc acactgcttt gttgtatatt aagaatataa
     6721 atctgataga aacccaaaga aaagaggata aaacaatgga tatagaaatg tttggtaagc
     6781 tttccgatgc atttggacca agtggatttg aggaggatgt catccgtacc atagcagatt
     6841 actgcaaaga atttgatgta gaaaatgacg caatgaataa cctgtatgtg cggatgccgg
     6901 gaacagagaa ggattcccgt ccggtcatcc agctggatgc ccatctggac gcatgcggtt
     6961 tcatggtaca gaatatccag gacaacggat gccttggcat tatcatgctt ggcggctttc
     7021 atctgaccag ccttccggca catgcggtat ggatccgcac cagatccgga aagatggtac
     7081 atggtattat ctgcgcaaaa ccggttcatt tcatgacaac agcggaacgc agcagtcaga
     7141 ctctggaaat tgaaaaaatg tatgtggatg tcggagcaac cagccgcgag gaggttgaaa
     7201 atgttttcgg aatccatatc ggtgatccga tgatgccgga tgtaaccttt gaatacgatg
     7261 cagagcatga gatctgcttc ggaaaagcat ttgacaaccg ggccggctgc gcatgcatcg
     7321 tagatacgat gaaacagctg gaaaaggagc aggcttctct tgcggttaat gttgtcggcg
     7381 cctttgcagc gcaggaggag gtcggaaccc gcggtgctgt ggtgacgagc cagattgtaa
     7441 aaccggacct tgccattgta tttgagggat ctccttccga tgactttttc atcagcgcag
     7501 gacaggcaca gggctgcatg aagaagggag ttcagattcg tattctggac aacagctata
     7561 tttccaatac gcagtttatt tcactggctg aggaagtagc agaaaaatgc ggaattaaat
     7621 accaggagag cgtaagaaga ggcggaagca caaacgcagc aaaaatcagc gtgacaggaa
     7681 aagcagttcc gtgtctggtg cttggtgttc catcccgcta tgcgcacagc cattacaatt
     7741 tctgcgcaaa agaagacctg gaggcagcaa gcgcaatggc agccaatgtc attcgtgctt
     7801 tagatgagga aaagatccgt catatctgcc atcaggatgt actgtaaaat attcgataac
     7861 gaaagaaaaa gcatgccgga agcgggttca caggaaaggt gaagccgttt tcggcatatc
     7921 tggtaaaaaa ggagagttta caagtgagaa aaccgatcat tggaatcttg ggaaatacgt
     7981 ataaaacaca gccgggactt ttttcaagtg cggagagaat gtatgtcaac agcgattata
     8041 tcgaatcagt tctgaacaac ggcggaattc ctatggcaat tccggcagtt gccatgaaag
     8101 cggatccgga aggcatactt gctgtgtgcg acggaattct ggttcccggc ggagaggact
     8161 taaatccgtg gtattatgga gaagagccaa aaccgcagat ccagaccatc cgcccggaga
     8221 tcgacgaggc atggtttgca cttggacggg cagcaaaaga gatgggaatg cccatgcttg
     8281 gcatctgcaa aggaattcag tttttaaatg tcctgtgcgg cggtgatctg taccaggata
     8341 tctatacaca gaaggaaact acaattctgc atctgcagtc cctggagcgg tcgtatctgc
     8401 atcaccatgt ggaaattaaa gagggaaccc atctggcaga aattctcggg gcgggaaccc
     8461 atgcggtaaa ctccatgcat catcaggcag tgcgtacgcc gggagaggac atcgtggttt
     8521 cagcaacggc gccggatgga accatcgagg cgatagaaag ccgcaacgga cagattgtgg
     8581 ccgtacagtg gcatccggaa gggctgatcc actcagcgcc ggagatgaac cgcctttttg
     8641 cggatctggt agaaagaagc agcagataca gagagaaaag gtaacgattt ggaaccatgt
     8701 aaaaggaaag aaaaaccggg atgggacttt tgaaccgtta cgaaaaaata agaatggata
     8761 aagatgcgat tccaaaagca gagagcagga atcgttcggg aggcaaaaaa agaatgaaca
     8821 gaacaataag agtatcggcg gcgggtgata ttctgattat gaaacggctt ctgccgggat
     8881 atcaggatgt tttgccgatt cgcgaatttc tgatgcaggg agaggttcgg atggcgaatc
     8941 tggaaacgac gatatcggac ggcagctgtt acgcatcggc gtattccggt ggaacatggc
     9001 tgacggcaga tgcgaagtgt ctggaagata ccctgcgtta cggttttaat tttcttggga
     9061 tttcgaataa tcatacgatg gattattcct acgaaggact tgcatctacg atcagcgaac
     9121 tgaaaaaaaa ggatgtggca tatgccggag caggcaaaaa tctttacgaa gcatcgcgtc
     9181 ctgccattct ggacacaacg gcgggacaga ttgcggtgat tgatatctgt tctacgtttg
     9241 agaatgcagc cagggcggga agccagacgg aacggcagcc gggccggccg ggcttaaatc
     9301 cgctgcgctt ttcggaaaaa tataccatta cggagaaaca tgcgcaggat cttgcaaaga
     9361 ttgcagaggt gaccggaatc aacggactgc gggatctgca ccggcaggag ggatttatcg
     9421 ctccgcttcc cgagggtgtc atggaatttg gcgggaaaat gtttgaaatc agcaaggacg
     9481 gaacagaggg aagaagctcc agcccgaatc cgatcgatgt gaaacgcacc gtcgatgcaa
     9541 tccgggaagc gaagatgaca tgtgaggcag tcctggttat ggtacattcc catgaaatcc
     9601 gtaaggacaa tgacgaggaa gcggattatt ttctggagga atttgcacac gcctgtatcg
     9661 atgcgggagc cagcgcggtg atcgggggcg gaacgcatca gctgaaggga atcgaactgt
     9721 atcatgactg tccgattttc tactgcctcg gaaattttat ttttgaaaat cagtatgtac
     9781 gccttctgcc tgcggactac atggaaaaat atggtctgaa tattcacacg gcagcatcca
     9841 tcggaatcgc caggcggcag gagcagtcct cacattccct ctacgaaatt ccggaggtct
     9901 atcggagtgt gctgccgtat ttcgagatct gtcaggggaa atgcacacat ctggagcttc
     9961 tgccggtcga gcttggtatg gacagggaaa acgcagaaaa aaatattccg tacgtggcag
    10021 acgaaaaaac agcagaaaaa attgcagaat atctcacccg cgtgagcagg agatatggaa
    10081 cagaatggga atacaaagaa ggaaggattg tacttcatgc gtaaaaaatg ggaaatagaa
    10141 gaggaatacc gaaacttttg ccggaacaac aaagaacttg cgctgcagac gctgcgggag
    10201 ctgacactga ccccgacgga aacgggaaaa gaggatcaga gaatcgcata ttgcatggaa
    10261 tggatgaaac agcagggcat ggagtcggtt catacggatg agcttggaaa tgtaatctgg
    10321 gagtatcggc cggaacagga aaagaaggtt ctgtatacgg cgcatctgga cactgtgttt
    10381 tcgctggagg aaccgctgga gattaaggaa gacggaatga tctggcgttg tcccggcatc
    10441 acagacgata ccgtgaatgt cgtgatgctt ctgatggcgg caaaatacgt tcatgagaca
    10501 gagccggagc ttccatgcgg tctgattttt gcagcggatc tgggagaaga agggcttggc
    10561 aatctgtgcg gagttcgcgc gcttgtcggc cattatgaaa aaaatctgtg cggaatggca
    10621 gcctttgacc tgtatcggga taaaatgtat cccatctgca tcgggtctgt ccggtatcgg
    10681 atttcggcaa aaacaaaggg cggtcattct ttcctgaatt tcggaagaaa aaatgccatt
    10741 gcagagcttg cgggtctgat cggagagctt taccgttttc agacagatgc agcatctcat
    10801 acaacataca atgtaggaaa aatagaggga ggaacctccg tcaatacgat tgcgcaggat
    10861 gcctccatgc tgtttgaatt ccgttcggag gattaccgga gtctggaagc ctgcgaaaca
    10921 tatctggagg agaccatagc agcacgccag aatgaggagg tacagtattc ctgtgagctc
    10981 gtcggaaagc ggccgtgcgc aagagaaacc gatcctgtcc agatggcacg tatgacgcgg
    11041 tgtgcacaga aaacgctgaa ggcagccgac ggggaagagc cggtatgctc tgaagcatcc
    11101 acagactgta atatcccatt gtcgcggcat attcctgcca tctgtgtcgg tttctgccgg
    11161 ggcgggggag cgcataccag agaagaatgg ctcgatgccg ccagcgtaga agatggcatg
    11221 tgtgcagccg tagcactcgt atgccggctt ccgtggatgt gctgcgagag cagagtcgtt
    11281 gtaagggatg ggatagagga cccaaaagag aaagaagaaa tccagcagct gctggagctg
    11341 tgtgatcagg atttcgttcc gccgctttcc caccgcaaca gtacatccca gacgaactgg
    11401 gccgagacag aagaaaagac ggatggaatt gcagaatatc tggagaatat ctgcagtcag
    11461 catgtggtgt tgtggaagga agagggagtc gtgcgggcct ttatgacctg gaaggatcac
    11521 tttaactgtg agaatctgga agcatatccg gattcctgct atctgaccac cctctgcgtc
    11581 tggccggatt accgcggaca gggaatttcg gaggtaatgt atgcagaagc agaaaaggac
    11641 atcgcagcaa aatttccggg aagccggatt actctccgta cctggtccac gaacggggcc
    11701 caggagcata tcctggacaa gctgggatat agtcttgtca ggcggctgaa ggatgaccgg
    11761 ggagaaggaa ttgatacggt ttattttgtg aaaaaggaag aaaacaacag ataacatggt
    11821 gcttgcaaat cgtagctgaa ggtgtcataa tcggaagcat aagacagcaa aaggagggct
    11881 atagctatgc cttgtacaac cattttagtt ggaaagaaag catcgtatga cggttccacc
    11941 atgatcgcgc gaaacgacga ttccggtgca ggccattata catcgaaaaa atttgtagtg
    12001 attcacccgg aggagcagcc gagaacctat aaaaccgaga tttctcatct gaccattgag
    12061 cttccggaca atccgatgcg gtacactgca gtgccaaacg cagaaaaagg cgagggtgtc
    12121 tgggcggcaa gcggcgtgaa tgcagcgcag gtcggtatga ctgcgaccga gacaatcaca
    12181 tcgaatccgc gtgtgctggg tgctgatccg cttgttgttt atcagccggc ggaagacgga
    12241 aaagaagagg ttccgggcgg aatcggcgag gaggaccttg tttatattgt tcttccttat
    12301 attaaaagcg ccagagaggg tgtcaaaaga ctgggcagtc tgctggagca gtatggtaca
    12361 tacgagatga acggcattgc atttcaggac cacgatgaga tctggtggct ggagacgatc
    12421 ggcggccatc actggatggc agtgaaagtg ccggatgacc attatgtggc catgccgaac
    12481 cagcttggca tcgaccattt tgatctggag gatgcactcg gcgggcagaa ggaatatatg
    12541 tgctcagcag acttgaaaga atttattgaa acatatcatc tggatctttc tatggatgga
    12601 aattttaatc cgcgcgatgc cttcggaagc cacgatgatt ccgatcacgt ttacaataca
    12661 ccgcgtgcct ggttcatgga gcgctatctg aacccgcaca cgaaaaaatg ggacggaccg
    12721 tatgccgatt atacaccagt atccgacgat attccgtgga tgatggttcc ggagaagaag
    12781 atcaccgtag aggatgtgaa atacgtactg tcctcccact atcagggaac accgtacgac
    12841 ccgtatgccg catacggcga taaatcgtgg aaaggggcat accgctccat cggtgtcaat
    12901 cggaccgatt tcctttctgt catccagatg cgcccggatc acaaaggaga caacggcatt
    12961 ctgcagtgga ttgcatttgc gtcaaatgca ttcaatgtgc tggttccgtt ctacaccgat
    13021 gtgacgacaa caccggagta tctttccaac acaacgggag aggtttccac agataacttc
    13081 tactgggcaa gccgcatggt ggcagcaatg gcagatgcat cctataagtc ttctatcttc
    13141 cacattgagc ggtatcagga aaaggtgatg gcaaaaggcc atgcactgat ccatcagtac
    13201 gatgcgctgc ttgcagggga gacagacgaa acaaaacaga cacaattgag agaagaagca
    13261 aacaacaaaa tcgctgaaat gctgaaaaaa gagacgggtg caacactgca caaggtactc
    13321 tttgaactca gcaaccagat gaaaaacgcg tattccagat cggatgcgta aggctttttt
    13381 gggggaaaga ataagagaat ggttctgcgc gcaggaaaaa ttgcgcgcag aaccatttcc
    13441 tgtccggaac cgcctcgaac aaacagggtg ttacagttcg tcggaaacca gtgaacgata
    13501 cgatcctgat aaaaaatctc agcgtacttt ggtgaatttt gttgacaaca ggcagttcct
    13561 gtgctacaat aaaccagcga catggaagaa ggtctttttt ttatgcctga aattgctgaa
    13621 gtacgagcag caaaaacagg gcagaaatga agagaccgcc gctgatccgc aaggatcaca
    13681 agcgataacg caaatacaca gtggaggtgg aagtcgtgcg cgtaaaaatc acattggcat
    13741 gtactgaatg caaacagcgt aattacaaca tgacaaaaga gaaaaagaac catcctgaga
    13801 gaatggagac aaagaagtac tgcagattct gcaaaaagca cacaatgcac aaagaaacca
    13861 aataatcttt ggtaagaggt gatccaatgg cagaaactga aaaaaaagca tccaaaaaga
    13921 gctggttcaa gggcttgaaa gcagagttca gaaaaatcat ctgggttgac aacaaaacgc
    13981 tgggcaagca gaccgttgca gtcgtagcga tctctgcagt aacctgcggg ttaatcaccc
    14041 tggttgacag cgctgtaatc gagttcgtca atttattgat caaataagga taaaggtgtc
    14101 agaatgtcag aagtaaactg gtatgtagtc cacacctatt cgggctatga aaacaaagta
    14161 aaagccaata ttgagaaaac aattgagaac cgtcatcttg aggatcagat tttggaagta
    14221 cgtgttccta tggaagaagt tgttgaagtc aagaacggaa ccaagaaaca ggtactccgg
    14281 aaattgtttc cgggctacgt tctgatcaac atgatcatga acgacgatac ctggtatgtt
    14341 gtccgcaata ccagaggtgt tacgggcttc gtaggaccgg gatccaagcc ggtaccgctt
    14401 acggatgagg aaatggtgaa ccttggaatc accggtggtg aggaaaaggt tgagcttgac
    14461 cttgaagtcg gcgatgcagt cgttgtaaca ggcggcgcat ggaaggatac aaccggactg
    14521 attcagagta tcaatgatac aaaacagatg gttaccatca atgttgagct gttcggccgc
    14581 gaaacgccgg tagaaataag tttcgcagaa gtcaagaaga tgtaagaaat gccccagggg
    14641 cgtttcgtgg gagggaaatt cccgcaatta ccacatttat ttaggaggtg ccgtaaaatg
    14701 gcaaagaaag ttacaggata tatcaaatta cagattcctg ctggtaaagc aacaccagca
    14761 ccaccggttg gacctgcact tggacagcac ggagtaaaca tcgtacagtt taccaaagag
    14821 ttcaacgcaa gaacagcaga taagggagac ctgatcatcc ctgtagttat cactgtatat
    14881 gcagacagaa gtttcagctt cataaccaaa actcctccgg cagcagttct gcttaagaaa
    14941 gcctgcaacc tgaaatctgg ttccggcgtt ccgaacaaaa cgaaagttgc tactatctct
    15001 aaggacaaag ttcgtgagat cgcagagatg aagatgccag acctgaatgc agcaactgtt
    15061 gaggcagcta tgagcatgat cgcaggtacc gcaagaagta tgggtatcgt tgtagaggac
    15121 taatttagaa gaagagttta catcttctcg tattgacgtg ggagggagat tcccgcaact
    15181 accaccagga ggttattaga caatgaaaca tggtaagaaa tatttagagg ctgcaaaagc
    15241 agttgatcgt agcattcagt acgatccggc agatgctatt tccacagtta agaaattagc
    15301 tactgctaaa tttgacgaga caatcgaagt tcacatcaaa actggttgtg acggacgtca
    15361 cgcagaccag cagatccgtg gagcagttgt tctgccgcac ggtaccggaa aacaggttcg
    15421 tgtacttgtt ttcgctaaag gcgcaaaagc tgatgaggct gttgcagcag gtgcagagtt
    15481 cgttggagct gaggagctga tcccgagaat ccagaacgat ggctggtttg attacgacgt
    15541 tgttgttgct acaccggata tgatgggtgt tgttggtcgt ctgggccgtg tacttggacc
    15601 gaaaggttta atgccaaacc cgaaggctgg taccgtaact atggatgtta ccaaagccgt
    15661 taaagagatc aaagcaggta agattgagta tcgtcttgac aaaacaaaca ttatccacgt
    15721 gccaatcgga aaagcttctt ttactgagga gcagttaagc gacaacttcc agacgctgat
    15781 cggtgcaatc aacaaagcaa aaccggctac tctgaaggga cagtacctga agagcgttac
    15841 tctggctcct actatgggac ctggagttaa ggttaatact ttgaagcttg cataataaat
    15901 gaaatactga gaacccccgg ggctgtaatg gctccggggg ttctttgcgt tgaaaaacgg
    15961 acgcagggga aaggtgaatt ccgattcctt aagaaagagc tttatttcaa tgggaaataa
    16021 ttgaaaaaga tcaaaatagt gttagaacat gaaaattaag atcaagtatt ctgccggaaa
    16081 tgttagtata atgttaagta gaaagcaaac gaaaacaaca gagcccgcgt gcgcttatgg
    16141 aacggcgggg gatggaaagg gagggtttta tgcagtattt tgagaaacag ggaaatgcgc
    16201 tgattttccg gcaggatggg gagacggtcc gggtggagcc gtggcagaag gacagtcttc
    16261 gggtgagagg tacgcttctt tcggagatag aagagggaag cattgcactg cttgatccgg
    16321 aaccggtgga agcggaaatt gagctggtgg atgaactgaa agcgacgatc aaaaacggta
    16381 aaatccaggc ggttcaattt ccgcttccac cggttccgga tcaagcagnn nnnnnnnnaa
    16441 atacaggcgg ttctcgagct tcagccgtgg ggacagaagc tgcggattac gttcctgaat
    16501 cagaagggcg aggtgctgct ctccgagatc gcaaacggcg gagcgctctg cctgcgcgca
    16561 cacgattacc gtgcattaaa gggaggcgct tatcagctga aagtatcctt cgattccaac
    16621 ccggatgaga agatttatgg tatgggacag tatcagcagg agagaatgaa cctgaaggga
    16681 tgcaacctgg aactggcgca ccgtaattcg caggcgagca ttccgtttta tgtatcgagc
    16741 cttggctacg gcttcctgtg gcacaatgca gcggtcggtg aggttcattt cggaacgaac
    16801 acaacggaat ggctggcacg caccacaaag cagctcgact actgggtaac cgcaggcgat
    16861 accccggcag aaattgaaga gcactttgca gatgccattg gtaaagttcc gatgatgccg
    16921 gagtacggcc ttggcttctg gcagtgcaag ctgcgttact acaaccagga gcaggttctg
    16981 aacgtggcgc gcgagtacaa aaaacgcggc attccgctgg atgtttttgt gatcgactac
    17041 tatcactggc caagatgcgg tgattaccgt tttgacgagg aatacttccc ggatccgaag
    17101 gcaatgatcg acgagctgca cgagatgggc attgagacga tggtttccat ctggccgcag
    17161 atcgactgga gaagtgaaaa ttacgaagaa atgaaacagc agggacttct tgtgaagagt
    17221 aatgcaggtg ttgatgttca gatgctgttc catggaaata atgtcttttt ggatgcaacg
    17281 aatccgagaa cgagaaagta cgtctgggaa aaagtaaaga aaaactatgc agatctcggc
    17341 atccgcacct tctggctcga tgaggctgaa ccggagtttt caacctatga gtacgagtgc
    17401 taccgctacg ccgcaggccc ggtggaagag atcggaaata tttacccgag agagtattcc
    17461 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngggcagg
    17521 aagatattgt caaccttgtg cgctgtgcat ggctcggaag ccagaaatac ggtgcgcttg
    17581 tgtggtccgg cgatattttc tcaacctacg aggacttccg caaacagatc tgtgcaggtc
    17641 ttcatatggg actttgcggt attccgtggt ggacgaccga tatcggtgga ttccacggcg
    17701 gtgtgaccga agatccggac ttccaggagc tgcttgtgcg ttggttccag tttggcacct
    17761 tctgcccggt tatgcgtatt cacggaaacc ggggaccaag agaagagatc atcaacaagg
    17821 ccggcgaagt gcgtgaggga accggagctg acaacgaggt atggagcttc ggagagaaaa
    17881 attatgaaat cctgacaaaa ttcatcggtg tccgcgaaaa aatgcgcgat tacacgagaa
    17941 gcctgatggc agaagcacac gaaaaaggaa caccggtcat gagaacgatg ttctatgagt
    18001 tcccggagga tgcagcgtgc tgggacatct ccgatgctta catgttcggc tccgacatcc
    18061 ttgtagcacc gattgtccgg gcaaaagcaa cgagccgtac cgtttatctg ccggccggtg
    18121 cgtcctggac gctggcaaat accggtgatg tttatgaggg cggaaaagcc tacgaaatcg
    18181 aggcgccgat cgaaacactg ccgatcttcc tgcgcgacgg aaaacaggga tatttggtgg
    18241 gagagatctg ataaatcaca gagcagttgt ttggttatgg gcaggcagca aatgcggatt
    18301 gcagatagct gcatgaaaaa agaaaaggaa aaagggaaaa gcattccgga agatcgacaa
    18361 gatccaaagg aatgcttttc cgatgttata aatcaattcc ggatccgaaa aacggatttt
    18421 agccttgctt cgcatacggg cggacagaaa aacttacgga agcggatgac ctgccgccag
    18481 atacagctca taccattctt cccgcgtcag cgtaatattg catgccgcga taatctcttt
    18541 caggcgtttt tcacttgccg taccggtgac gagctgcata ttcgccggat ggcgcagaat
    18601 ccacgccgct gcaatcgtgg tatcactgac accgtatttt tcggccagca cgcggatttt
    18661 ctggttcagc tccggatact cgtcacttcc aaggaagcat cccttccatg acggcatctg
    18721 gaatggcgac cacgcctgaa gcgtaatatc atggagccgg cagtagtcga gcagacttcc
    18781 gtcgtggtca accgagcccg gtgtctccat attaacttcc atgccgttgg cgacaagatt
    18841 ggaaaccgga atgctgaact gtacctgatt gacaacgagc ggctggcgga catatttctg
    18901 caaaagcgca atctgcatcg gcttgtggtt ggaaacaccg aaatggcgga cctttccgct
    18961 ctcaaacaga acatcaaacg ctgctgcaac ttcttctggc tctacgaggg cgtccgggcg
    19021 gtggagcagc agaagatcga gataatcggt ctgcagccgt tttaaaatac cgtccaccga
    19081 ttccagaatg tgctctttcg acagatcata gcagccctgg cggatgccgc atttggactg
    19141 gatcagaaca tcctcgcggt gcagggaagg atcggaggca aatgcatcac cgaaaactgt
    19201 ttcactcatg ccgctgccgt aaatatcagc atgatcaaag aaatatgcgc cctgttccag
    19261 cgcggaatgg atgaaatgat tcatctcgtc tttcgatttt tcggaaagcc gcatgcatcc
    19321 gactgcaatc gaaggaactt ttctcagatg actgcctaaa accgtttctt tcatgacaga
    19381 attctccttt gtgataaaat aaaatcttac tctcactata ccactcaagt acacttgaga
    19441 gtcaatcttt tttgggaaaa agtggtgaaa gctttcgcca gaaccgctgc ggattcgacc
    19501 aaaacacaga aaagccgaac tttgttaaca aaatcggaac aaaatcgaca acaaagttta
    19561 cttgcatttt cgaaaaaatg atgtaagata ggggtattcg aaaaaagatc cgaaaggatc
    19621 tgtaaatctg tactgactca gataatggag gtttacccat ggatgcaaaa atcatagccg
    19681 gaatcctggc agcaggaggc gcagccttag ctgccggcgg cgtatataga atgaataaga
    19741 aaactggtta cttcaaaaaa ggaaactccg tccggtacga tgtttcccgg atcccgttta
    19801 aaaagacgag tccgttaaaa ggaaagacgg ttgtcttcct gggatcttcc gtgacgaagg
    19861 gatttgcggc ccacaacaat gcgttcgcgg agtacatagc gaaaaaagac agctgtatct
    19921 gcatcaaaga agcggtcaat ggaacaacac tgattgataa ctgtgaggat tcctacatcg
    19981 agcgcatgcg cgacaatctc gacccggagc gtcaggtgga tctgtttatc tgccagcttt
    20041 ccacgaacga tgcaacgcgc aacagcccgc tcggcgaaat ttcggaatcc cgtgatctgg
    20101 acagcttcga tgtcaaaacc gtctgcggtg cgatcgagta catcattgcg tatgcaaaag
    20161 aaacgtggca ctgccgggtc atgttctaca ctaacccaca gtatgattcc gatacgtacg
    20221 caaagatggt ggaagcactt ctgaaaattc aggaaaaatg gcagatcggc gtgattgatt
    20281 tctggaatga cgaacggatc aacagcattt cgccggaaaa acggaaactg tatctccagg
    20341 atccgatcca tccgatgaag gccggctacg ctgagtggct cgtcccggtg atggagcagc
    20401 ggattgccga atatctggaa acgcaggtat cataaaaaag acaaaaaaat actttcgtgg
    20461 tttctttggt tggaagattc cgcgaaagta tttttttatt gcgctgccct taatggagcc
    20521 ggatgatcag gctgctgtgc tgacgaaatt gttttttccg tactttctgt tacgttccgt
    20581 tttttacgga tccggaactg tttgaaaatg ccgcggatgg cgtcgtagag ctccggcttt
    20641 aagtcctcaa gcgggattgg ctgctgatac aggatggcat tgtagcttgt gccgcttacg
    20701 agttccagaa tcatatagaa catcagctcc ggtgagcggt acgtatatgc cgcgtgagag
    20761 agcagcgatt cgtaaattgt gtagatagca ggtgcgtcac tgaacggaga aaatgtaagc
    20821 gaatttttga agaaccccca gcttaagtgc ttggagatca gcgtcacaag gctgtggttg
    20881 gcagcaaact gatcgaggat attgtcggtg atgaaaagca cctgctcctc aaaatcctgg
    20941 atgtccggat ggcgcagcag atccgcatag gctgcctgga atatctggct tgccttgtgc
    21001 gcaatcagat gatttcgaat atcgtattta tctttgaaat aaagatagaa agtgcccttt
    21061 gcaacgccgg cgttgttcac gatgtccgaa atagaggttt tattaaagcc gttgtcgata
    21121 aagagcgaga aagcagagtc cagaagggac tcacgcttca tttgtttgtt attatcaatt
    21181 tttcccacag agagtctcct ttcctgaaag atatcaaaaa cgtgtgtcat tatcctgcct
    21241 ataattatag gtatcataca tggaaactgt caaagggaaa atgactaatt ttctcatttt
    21301 tgtgactatg cacgaagaaa aaaatgaccg atcgtcaatt ttgtgcagta tttaatgttg
    21361 actaagagtc atttttgcgt tataacatta gatgattgaa aggcatagga aaaacatcct
    21421 cgatctgagg gaaacagtat cccgcagtat cttactccga gcgggtttta cttctcttat
    21481 gatcctggat ttcatcataa aaaaccatta taaaaaccac agaaaggaag tttatcacaa
    21541 tgaatagaaa cagaaaactg agaaaacaga tgatgtctgg tcttgctgtt ggtatggctg
    21601 ctgttatggg agctgctccg gtttatgcag cgacaggaga ggacgcagcg gtttccaaag
    21661 aagaaaccgt atatgtaaat gcaaatgcag acggatcgga agatcagatt accgtatcca
    21721 actggctgaa gaacgcagga acggaaaaga acctgcaaga tgagagtgaa ctgaaggaca
    21781 tcaaaaacgt aaagggcgac gaggagttca cccagagcgg aaacaagctg acctggcaga
    21841 cagacggcga ggatatctat tatcagggaa caaccacaaa agagcctccg gtttccgtga
    21901 agttcaccta ttatctggac ggcaaggaaa tcaaaccgga tgacctggcc ggaaagagcg
    21961 gaaaactgaa aatcaaagtt gactatacaa acaacacaaa gacaacggtt gacatcaacg
    22021 gcaagaaaga gacgatgtac accccattcg tcctgatgac cggaatgatt cttccgcagg
    22081 atactttctc caatgtcaag atcgacaatg gtaaagtcat ttccgatgga agcagaaaca
    22141 tcgtaatcgg atttggtatg ccgggcctgc aggaaagcct gggattaaat tccgattccc
    22201 ttgaagatct ggtatccggt gacagcgatt tcgaactgcc ggaaagcctg gagatcactg
    22261 cagatgtgga gaacttctct ctggattcta ccttcaccgt tgcacttgac gatgttctcg
    22321 acaccatcga taccgacaac atcaccggat acgatgacct gaaggatgca ctgagtgatc
    22381 ttgaggatgc tgcaatggaa ctggttgacg gttccaaggc actctccgat ggtgtggata
    22441 ctttaaaaga cagctacaaa gagctggatg acggtattgg aacgttaaaa ggcggcgctg
    22501 acgagctgaa cagcggagcc ggcacactgg caggcggagc cggcacactg gcaagcggag
    22561 catcgactct ggcaaacgga acttctactc tgaacagcgg agcttctacc ttaaacagcg
    22621 gagcagctac cctgtccaac ggagcatcca ccttaagcag cggagcttct tccctggcaa
    22681 gtggcgtaaa ctcctacacc gagggcgctg ataccctgaa taaagcaatc cagacgtatc
    22741 ttggaaagaa cggtgtgctg actggaagcg taaccgagta tgtaaacggt gtgaataaag
    22801 tcgttgcagg cgtaaaacag tacaccgacg gaacaacaca gctgtccgac ggcgttgctt
    22861 cttatgtagc aggcgagaaa cagcttgcag caggcgctga gaagcttcag cctctggcag
    22921 acggcctgac cgaggtaaac agtgcaatcg caacactgaa cgcagcactc gacggaaaag
    22981 atgagaatga tattttagca ggagcaaaac agctctccga aggactgacc agtttgaacg
    23041 agcagctgca gagcaccgag gtacagagcc ttctgacgat ggtagacggc atgaccagca
    23101 ccggttctac cctgatctcc gagaccgagg cactctccga ggttctgaac accacaatcg
    23161 caggatctgt caacagcatg atgacaaacg gcaacgagct gatgagccag atggatacgc
    23221 tgtccaaagg acttgagagc tacttaagcg gtctggaggc acagatcacg aatcaggcaa
    23281 cgcagatggg aacccagatg gcagcacagg ttcaggcatg ggcagacgga gcagcagcac
    23341 agctgaacca ggaagcaaca gcagcagcac agagcgcagc tgatgcagca gcaagccagg
    23401 catccgccga tgcagcaaaa caggcaaatg atcagctctc cgcagcagcg gctacactgg
    23461 atgcacagat ttccgcagct aagagcgccg gaaatacaga gcttgccgat tccttaagta
    23521 aagcaagaaa ccagatcgga agtgtcagcg tttccgcttc agcaggaacc gtttcggtta
    23581 ctccggttac agctccgcag attactcttc cggaactgaa cttcgaaatc gagcgcccgg
    23641 atctctccgg tcttgcagca atgcagcaga agatgagcga catcaagagt cagtacagtg
    23701 atctgcagaa gacggttgca gctctgatgc cgcagcttga gaccatgcag aaacagatgc
    23761 agaccatcac cgataataaa gcagttgttg agcagaatct tggtgcaatg cagaaactga
    23821 ccgcaggtgt ggcacagctt gatgcaggcg gaagcgctct gtacaagggc atcggtactc
    23881 tgtccgcagg tgtgagccag ctgtacagca gcacaaatga gagttttgca aaagcaacag
    23941 caggcatcaa tgcactgaac gcaggattca ctcagctgag tgcttacaat gatacgctgc
    24001 tttccggcgc aagccagctg aaagcaaatg caccggctct ggttgagggt gttacgactc
    24061 tgcagggcgg cacgacacag ctgaccgcag gtcttggaca gctgggaagc cagttaagcg
    24121 atggatctgc aaaactttct gcaaacagcg gaagcttaag aagcggtgct tctcaggtag
    24181 caagcggagc atcccagctg gcaagcggag cctccacact ggcaaacgga acttcttccc
    24241 tggcaagcgg agcttctgaa ctgaacagcg gagcttctac gctggcaagc ggagcatcta
    24301 cgctggcgag cggagcttct acactggcaa atggaacttc ttctctggca agcggagctt
    24361 ctgatctgaa atcgggaagc agcaaggttg ttgatggtat tgacgagctg tccgatgggg
    24421 cgaaagaact ggctgacggt acgaagaagt ttgaagaaga gggtaccagc aagctgaaga
    24481 gtacctttga ggatgatctg ggagatgtac tggatcggat tcaggcgctg aattcggata
    24541 aatgcgctta tacttcttac agtggtaagg caaatggaat ggatggaagc gtgaaattta
    24601 ttatcgagac tgcagcaatt gaagctgcgg aagagtaatg tgattccacg gtagattgtg
    24661 aggccgcctg cggtcgggat gttttccccg accgcaggcg gcatttacgt tacgtggggt
    24721 gcaaaaagac aaaaaataag aacagcccgc ggatatgcgg gctgctcttt cataatgagg
    24781 ggggagatag ttttgagtgg ttcatcaact atctaagtta tagtataggg aagaaatgtg
    24841 agcaaagtat gtctattttc taaaagaaac agtaaatttc taaagaataa gtgaatccat
    24901 ccattgagta aatcacttcc gctcgccaaa acgtagatgg gtccccaggc gcagcggagc
    24961 gtccgccccc cgcggtgcat ttgcaggagg ggtaaggatg ttagagggcg gaaatttgcg
    25021 ttgggaccgg gcgcagcact gtctgaccga agggagtttg cggagcccgg ttccggtttt
    25081 agcaaatttt cggcctctta cagcctcttt ccctccgaaa cagcaccgtg ggggcggacg
    25141 ctccgctgtg actgggggtc gtatcctccc gtcctattca aaagaaagtt tccggttccc
    25201 ataactcttc cggtactgtc ccggcgtcat cccggtgagt tttcggaaag ccgtggaaaa
    25261 attatgcgca tcctgaaatc caacattata ggcaatctga gagactgtgt gattcgtgtc
    25321 cgtcagctca gccattgcgc tgtccatttt ggatttcagg atgaactgct tcagtgtcat
    25381 cccggtgccc tcgtggaaga gggaggagag gtatttgtcg ttgtagccga aataacgggc
    25441 gagttcggtg actttgaggg actcgctgct gtggtactgg atgtagctga tgatgtcctg
    25501 caggatctgc gtccgctgcg tttcggaagt gtaggtgcgg tagaaggggc tctgacagtt
    25561 gatttcgccg agaacagcgg cagtcaggct gttgttgagg aacgtgttgt ggaaacgcag
    25621 ggaagtgtcc tggagctgct tcatgaggat gatgacgcgg ttcggggacg aaagcgttcc
    25681 ctggcgcggg atgcataacg tttcggggta gttggatgga agctgggcgg gagcgaggtc
    25741 ctcaaggcgg taggaggatg gcggtgcagc atgaaactca tccagaaccg atccggcggt
    25801 cgggtcagaa ggagcgatga tcggtgcgga ggggatacca gggataaaat ggagccagta
    25861 gaaagcgcag cttccttttt tcgagccgtg ctgatggacg gtcggcggca aaagaagata
    25921 ttcgccgggg tgaacctcgt aagagcggcg ttcgtccgcg ataaaaagcg ttccttccgt
    25981 cacaaccatc agttcaaaat tgacgagatt ccgtgtcagg tgcatccact ccggggacgg
    26041 ggactggaat ttgccgcaga ggtcgtaggt aaaacaggcg gcatctggaa ataaaacaga
    26101 gatcatatga ctttttcaaa ccttttcaaa cattcttgta ttttaaacgg cggtccgttt
    26161 tgctaaaata aaactataga aaaacaggaa aacagccgtc aaaagaagtg taacgcttcc
    26221 catatctgaa agcaaggaaa atatcttttt caaaccagga ggcggcggaa gaaaatgaaa
    26281 aaggagaccg aaaaaatgga tcagaaaaac ttcagcaaac cattatccct ggcaaaagtc
    26341 caggttaccg atgcattctg gaaaaaagag atggagcttg tccgcaccga agtaattccg
    26401 tatcagtgga atgccttaaa tgacaatgtt ccgggagcag caccgagctt ctgtatgcgg
    26461 aactaccgcc gcgccggtga agttgaaaaa gagcggaaag caaaaggaga caaatttgtt
    26521 cagatcaaat atccgctgga tacttttgag acactgccga aggatggcaa aatggacgga
    26581 agattttatg gcttcctgtt ccaggatacc gattttacca aatgggtgga ggcagttgcg
    26641 tactccctga cacagcatcc cgatccggag ctggaaaaaa cggcagatga agcaattgaa
    26701 gctgtctgcg cagcacagag agaagacgga tacctcgata cctactatct gatcaacgat
    26761 caggatatga tttttacaaa ccttaaggac aaccatgagc tgtactgctt cggtcatctg
    26821 acggaaggcg ccgtagccta ttatcaggcg accggaaaag accatctctt aaaagcagca
    26881 gagcgtttcg cggactttat tgccagcaaa ctcggtcacg gcgagggtaa aaaagcaggt
    26941 tatcctggtc acgaaattgc agagatggct ctgatgcgtc tctatgatct gaccggcgag
    27001 cagaagtatc ttgatcttgc aaaatacttc atcgatgaac gcggaacaaa accatactat
    27061 tacgatatcg agcgcggtct gacctgcccg gagggcgagg aacgatatag ctacaatcag
    27121 gcaaaccgcc cggtgcgtca gcaggatgag gtgcaggggc actccgtacg cgccgtatat
    27181 ctctattccg gcatggcgga tgtggcaagg gcaacgcagg atgagagcct gctgaaagcc
    27241 tgtgaaactc tctggaacaa catggtgcat cagaaaatgt acgtcaccgg cggaatcggt
    27301 gccactcata taggagaggc attctctttc aactatgacc tgccaaacga taccgcttac
    27361 gcagagacct gtgcatccat cggtcttgtg ttctttgctc gccgcatgct ggagattcag
    27421 gcaaaagcag agtacgcgga tgtgatggaa ttagccctct acaatggcgt tctctccgga
    27481 atggcgctcg acggaaagag cttcttctat gtcaatccgc tggaggtact gccggaggca
    27541 tgccacaagg atgagcggaa attccatgta aaaccgatcc gccagaaatg gttcggctgt
    27601 gcctgctgcc ctccgaacct ggcaagaacg gtaagctcag ttgcatctta cgcatacacg
    27661 gaaaacgaca caaccctgtt tgtccacttg tacatgggtg gaacagtgga aggagaaaaa
    27721 gtaaaagcat ccatcacttc cgaattcccg tgggacggcc atgtttccgt gacctgcgag
    27781 agcgatacca aagagccgta cacctttgca ttccgcatcc cgggatggtg tgcttcttat
    27841 gaaaaagaga ttccaaaagg cgcagaagtg gaagaaaaag acgggtatct gtatgtgaca
    27901 aaagtatggg cgaagggtga gacgatccgc ctgaacttcc cgatggaaat ccagtttttg
    27961 gcatccaatc cgcttgtacg cgaggacgcc ggccgtgttg ccgtaaaacg aggaccggtt
    28021 gtttactgta tggaagaagc agacaacgga aagaatctgc atttgacaaa actctgtgtg
    28081 aatggtgaaa aaaccgcaga agcagccgat gagctgggcg agcactttgt gcgcgtaacg
    28141 gccgagggac gtcgcatgaa gctgggcgat gcggaaaaac agccgttgta tcatctgtat
    28201 cagaaagagg aagaggaaaa gacgaaactg aagctgattc catattatat gtggaacaac
    28261 cgaggtgagg gcgaaatgca ggtatggatc cgcgcatgtg aatgatttta ttaaagaaga
    28321 aataaaaaac tccggaaaag ggatggctcc tgatgcagcc tccccttttt tccggagttc
    28381 ttgttatgcc aaaatatgag aatcctgcaa aaaagaaagt gaattttttg caggattctt
    28441 tgtgctattg gtacagatta ttttctgctt ctcatagaaa gactgccgaa agcagaagaa
    28501 acggcgggca gaatcacgag gacgactgca aggacaatca ttcgatccac aaagtctgtc
    28561 agagcctgta caacgcagac ggacagcgtc agattcatac cgaggctgtg aagcagctgc
    28621 accagcacag tggagccgga ggaagtaatt ccaccgaaaa caaccgcggt gattgtggaa
    28681 cttaccacag tacccggaag tgagatcaca agcgctgccg gaaacagctt ccagcccatg
    28741 gagcggaggg aaagactctc gctgcgggat cctgccgtat gggacgggcg gagtctgcgg
    28801 aatacaaggc cggcaacgag accgatgaca agctgcaccg gaatatagta aaacgcatag
    28861 acatcactcg tcatgccgga aatgagtccg gagatcagtc ccggaacgag gcctgcagca
    28921 ggtccgaaca gagcggctgc cagaactgtt ccgatggagt ccagataaac cggaagacgc
    28981 agcagcagag caatggtacc gccgagaata ttgatgacac cgcagagcgc gagtgtcgtg
    29041 agctttaacg tacggttagt attcatcatg cctcgcctcc cttctgaaac atcgtctcat
    29101 gtgggaagac cggcggtgcg gtgagaatct ggtcgagaac cggtgcggat tcctcctgcg
    29161 gcacgccgag aacgcgctca aagaacatca ggaagaaacg gcgcacatcg acctgcgtca
    29221 gaatgtggct gttcggtgct ttctgataaa aattcatggc atccacaacc gtctgtccga
    29281 tgcagaggcc tccggtttct acctgcgtat acgcatcaaa accgctgcag agcgacggat
    29341 ccacaagata agcggccgcg agcggatcgt tgatcacgca tccgatgacc ttttcctgtt
    29401 tccagtggaa atcaaagtag aagcgcgtca gctcctcaat gcgggttccg actcctggat
    29461 ttacgcattt catatattcc acaagattcg gcgtcaggac aatttttcgt gtgacatcca
    29521 gcccgatcat atgaagcttt ttgcctgcaa gctgcggcag ctcggaaaac gcgcggaata
    29581 cttcggctgc ggcatccggg tcgcaccagt aattgtactc ggcaaccggg gagcagtttc
    29641 catggctctt atagcttccg cccatggaga caagagccgt caggtttgtc aggcactccg
    29701 gtgcgcggga gatgactttt gcaaggttcg tcatcggtcc gagggcaaga atggtaacat
    29761 caggggttgc ttttaaggtt tcgatcagga aagaaacagc gtccttgttt ggcgtaaggc
    29821 cttcggcatc cggccagccc gattcgccga gaccgtccat gccgtgggtg tccatcgcgt
    29881 ccacgtagtc gcggaccaga ggcgtctcct cgccgacata aaccgggaca tctggttttc
    29941 caatcataga aagaattttg agggcatttt tagcgcccat agaagcgggt acatttccgc
    30001 tgactgtggt gatgccgaca agatccagct ctttcgaagc cgcggcaagc atcagggcga
    30061 gagaatcgtc gatgcccgga tcacagtcga tgatcagttt ttttgcattc attttccaat
    30121 ctcctttgat gaaaattttg agatacttgg ttttttatac tgggaagttc acgaaccagt
    30181 ccggacaccc gcaaaaagac gggaaaatcc gattgcataa atgcacgggc aagagtatag
    30241 cacagataaa aaaaaaaatc cagtaaaaca aaccttgcct gttttgaaaa aatcggttat
    30301 ggtatagaaa agcatacagc aaatgagaac atgggagaaa agaaaatggc agatggattt
    30361 ttagcaccaa ctgggaaatt ctatcccaaa acagaaaatt ttcatgcgca gaccgcgcgg
    30421 gcgattttag gtccggacgg ccagacggac gagccgatcc aggagctttt gcgccgcggt
    30481 tatattttat ttgtaggatt ccacaaacca ggcgaaccgg aaaatctcca tgccgatatg
    30541 gattacgtcc tcggcggacc ggggtatccg gcgaccgaag ggcagaaggc ctggattgca
    30601 gaacatacgg aagagctgtc gagaaagcag cagtttgaca tcaacaacga cgagacaaac
    30661 tttgagaact tttacatcag caatgtccgc atgtttccct ggtgcaaagg gtgtgccgaa
    30721 gagaaagcgc gtgacctctg gggcaatgca cagagcgagg agaagccgaa gcggtgtgat
    30781 gcgtgtccgg ccttcaggaa ccgtccgctt taacgaaaaa cagaaaatgt cagaaaataa
    30841 tctggtgaga ttataatagg aagaaaaaat acttttgcac agtaatgcat caatggggga
    30901 aatccgcaga agccgcgtaa atacgcgatt ttttgcgaat ttcccccttg acttttgaaa
    30961 ggacagacgc tataataaac gagcgtgcgt aaaagactgt tttttgtgcg cgcaaaagct
    31021 gcaaagcaat tatgttgaac taaagcagcc gtatgaaacc ccgtgggaca cacggagaaa
    31081 tctatcagga ggtgaaatgg aatgccaaca ttcaaccagt tagtaagaaa aggccgtgag
    31141 acttccgtaa agaaatctac cgcgccggca ctgcagaaaa gctacaattc tctgagaaag
    31201 aaatccacag atatgtcttc tcctcagaag agaggtgtgt gcacagctgt taaaactgct
    31261 acaccgaaaa aacctaactc agctcttcgt aaaatcgcca gagttcgtct ttccaacgga
    31321 atcgaagtaa caagttacat cccaggagaa ggtcacaacc ttcaggagca cagcgttgtt
    31381 ctgatccgtg gaggaagagt taaggacctg ccaggtacca gataccacat catccgtggt
    31441 acacttgata ctgcaggtgt tgctaacaga aaacaggccc gttctaaata cggtgctaag
    31501 agaccgaaaa agaaataatt aagtaaaacg agttcgagat atatctagta tcacaaaaaa
    31561 gcgaaatgtt gtgcgttatt ccataaatcc ccacggctgc ggatttatag agaatttaca
    31621 cgcgcggaca tactgcaaat aagtgtgagt accgatgatt taatcgaatg atggttaagg
    31681 agggaagtaa cgtgccacgt aaaggacata ctcagaaaag agatgtttta gcagatccgt
    31741 tatacaataa caaagtggta accaagctga tcaacaacat catgctggat ggtaagagag
    31801 gcgttgccca gaaaatcgta tacggcgcat ttgctaaagt agaagaaaaa gctggaaaac
    31861 cagcaatcga agtatttgaa gaagcaatga acaatatcat gccggttctt gaagtaaaag
    31921 caagacgtat cggtggtgct acttatcagg taccgatcga ggtaagaccg gacagacgtc
    31981 aggctcttgg ccttcgttgg ctgaccgctt attctcgtaa gagaggcgag cgtaccatgg
    32041 aagacagact ggcaaacgag attctggatg catctaacaa cacaggtgct tctgtgaaga
    32101 agaaagaaga agttcataag atggcagagg ctaacaaggc atttgctcac tacagattct
    32161 aagaggagga aaatccgttg gctggaagag aatatccatt agagagaacc agaaacattg
    32221 gtattatggc tcacatcgat gcaggtaaaa caaccctgac cgagcgtatc ctgtactata
    32281 ccggtgttaa ctacaagatt ggtgatactc acgaaggaac tgctaccatg gactggatgg
    32341 aacaggagca ggagcgtggt atcacaatta cttcagctgc tacaacctgc cactggactc
    32401 tggaagagaa ctgcaagccg aaaccgggcg ctcttgagca ccgtatcaac atcattgata
    32461 ccccaggcca cgttgacttt accgtagagg tagagcgttc tcttcgtgta ctggacggcg
    32521 ctgtcggcgt gttctgtgca aaaggtggtg tagagcctca gtctgagaac gtatggcgtc
    32581 aggctgatac ctacaacgta ccgcgtatgg cattcatcaa caagatggac atccttggtg
    32641 ctgacttcta caacgctgta gatcagatca agaccagact tggtaaaaat gctatctgcc
    32701 ttcagcttcc aattggtaag gaagatgaat tcaagggaat tatcgatctg ttcgagatga
    32761 aagcttacat ctacaacgac gataagggcg atgatatctc catcgttgat atcccggaag
    32821 atatgaaaga cgacgctgag ctgtatcaca ccgagctggt agagaaaatc tgcgagctgg
    32881 atgatgagct gatgatgcgt tatctggatg atgatatccc gtctgtagac gagatgaaag
    32941 cagttctgcg taaagctacc tgcgagtgta cagctgtacc ggtatgctgc ggttctgcat
    33001 acagaaacaa aggtgttcag aaactcctgg atgcgatcct tgagtacatg ccggctccga
    33061 ccgatatccc gccaatcgag ggtaccgatc tggacggaaa cccgatcgta agacattctt
    33121 ccgatgacga gccgttctcc gcactggcat tcaagattat gacagaccca ttcgttggta
    33181 agctggcata cttccgtgtg tattcaggaa ccatgaactc cggttcttac gtactgaatg
    33241 caacaaaaga taaaaaagag cgtgttggac gtatccttca gatgcatgca aacaaacgta
    33301 tggagctgga taaagtttac tccggtgaca ttgcagctgc aatcggattc aagttcacca
    33361 caacaggtga taccatctgc gacgagcagc atccggtaat ccttgagtct atggagttcc
    33421 cggagccggt tatcgagctg gctatcgagc ctaagacaaa agcaggccag ggcaaactgg
    33481 gcgagtccct tgcaaaactg gctgaggagg atccgacctt ccgtgcacac accaatcagg
    33541 aaactggtca gaccatcatc gctggtatgg gtgagctgca tctggaaatc atcgttgacc
    33601 gtctgcttcg tgagttcaaa gtagaggcta acgtaggtgc tccgcaggtt gcatacaaag
    33661 agaccattac caaaccggca gacatcgaca gcaaatatgc aaaacagtct ggtggacgtg
    33721 gacagtatgg tcactgtaaa gttaaattcg agcctatgga tgcaaacggc gaagagattt
    33781 acaaattcga gtctaccgtt gtcggtggag ctattccgaa agagtatatc ccggctgtag
    33841 gcgaaggtat cgaagaggct atgaaagccg gtatcctggg aggcttcccg gtagtcggtg
    33901 ttcatgcaaa tgtatacgac ggatcttatc atgaggtcga ttcttctgag atggcattcc
    33961 acattgctgg ttctctggca ttcaaggaag ctatgcagaa gggtgctccg gttcttcttg
    34021 agcctatcat gaaagtcgaa gtttccactc cggaagatta catgggtgat gttatcggtg
    34081 atatcaactc ccgtcgtggt cgtatcgagg gtatggatga catcggcggc ggaaagatga
    34141 tccgtggatt cgtaccgctg gctgaaatgt tcggttacgc aacagacctt cgttcgaaaa
    34201 ctcagggacg tggtaactac tccatgttct ttgataaata cgagcaggtt ccgaaaaacg
    34261 tacaggaaaa agttctgtct gcaaaagcaa aataatgtaa gaaaaataat cgggtgtgca
    34321 gctgtcaaag gctgcacagc ctaaaagtat tatttttctg agatttccct tgcaaataag
    34381 acggaaatgc aatataatca gacttagcag gtttacgatt caggtgggtt cctgccacca
    34441 atttagtctc aaaaaagccc actgggcgca attttaagga ggacatttta aaatggctaa
    34501 agctaaattt gaaagaacaa aaccgcattg taacattggt accatcggcc acgttgacca
    34561 tggtaaaaca actctgaccg cagctatcac aaaagtactt tctaccagag tagaaggaaa
    34621 cactgctgag aacttcgaag atatcgataa agctccggaa gagagagagc gtggtatcac
    34681 aatctctact gcacacgttg agtatcagac agagaaaaga cactatgcac acgttgactg
    34741 cccaggccat gctgactatg taaagaacat gatcactggt gctgcacaga tggacggagc
    34801 tatccttgta gtagctgcta cagacggtgt tatggctcag acaaaagagc acatccttct
    34861 gtcccgtcag gtaggtgtac cttacatcgt tgtattcatg aacaaatgcg atatggtaga
    34921 cgacgaagag ctgcttgagc tggtagacat ggagatccgt gagctgctga acgagtatga
    34981 cttcccggga gatgacactc cgatcatcca gggttctgct ctgaaagctc tggaagatcc
    35041 ggctggccca tggggagata aaatcatgga gcttatggat gcagtagata gctggattcc
    35101 ggatccgcag cgtgacacag ataaagactt catcatgcct gtagaggatg tattcaccat
    35161 cactggtcgt ggtactgttg ctactggccg tgttgaggct ggtgttcttc atctgaatga
    35221 ggaagttgaa atcgcaggtc tgaaagaaga gaccaagaaa actgttgtaa ctggtatcga
    35281 gatgttccgt aaactcctcg atgaggctca ggctggtgat aacatcggtg ctctgcttcg
    35341 tggtgttcag agaacagaga tcgaaagagg acaggttctg gctaaaccag gtaccgttca
    35401 ctgccatacc aaatttaccg ctcaggtata cgttctgaca aaagacgagg gtggccgtca
    35461 tactccattc ttcaacaact atcgtccgca gttctacttc cgtacaactg acgtaacagg
    35521 tgttatcact cttccggaag gaactgagat gtgcatgcct ggcgataacg tagagatgac
    35581 catcgaactg atccacccga tcgctatgga tcagggtctt accttcgcta tccgtgaggg
    35641 tggccgtacc gttggatcag gccgtgttgc tactctgatc gactaatttc ggttatctat
    35701 gagcagtgca aattcggaga gtaatgaccg gtgggagctt gctcacacaa gggcattagt
    35761 cggagaattt atagggcgac agcccgaccg agatggagcg aagcgaaatc gaggaaagca
    35821 ctgcgggaaa taaaatataa ttgtatcgaa agatacttgt gctcaaacgt aagagacccc
    35881 ggcgccgtaa ggcggccggg gtttttctgt ttttaaccaa aagaaactta tcatatatag
    35941 caggatatga tataattttt attagttgaa ataaaagaga ataagaaagg aaaaaaggat
    36001 ggactacttg gaggcaattg atgaaacctc ttatttatca gtaccgaatg caatggagag
    36061 aactgctgca gtgcgtggga gttgtgccgg ataatatttc ttccatggta catgcgtttg
    36121 gaattcgttt gaaaaaacag gagatatggc atcctgcata tgaagccttt tgccgatgcg
    36181 gggaaccgta tgtgcttacc atggaaaatc tgaagggtat tacagaagta cagcctgtcg
    36241 gaacgtgtgt atacattgtg gaaaacgaaa tggtattcag ctatcttatg gaacaggtgc
    36301 agggaaagaa tgtgtcgctg ctgtgtacgt cgggacagcc gcggtatgca gcattaaaac
    36361 tgatttcgtt gatcgtacag agtgggattc tcatttacta cagtggagat cttgatccgg
    36421 atgggatcgg aattgccgac agactctggc agcggttcgg aaacagaatc caattctttg
    36481 gaatgtcacc agaggattac cggaacagtc tttcaaaaga agtatttgga gagaatggca
    36541 gaaaaaagct ggaacacatt tggcacccgt tattaagaga aacggcggaa ctggtaagga
    36601 aaacaggaaa agcgggatat caggaaaata ttttaaaaga gctttccgag aagcttgtcg
    36661 gatgtgatca aaaccagaat ctgtaaaata cgaattgtga atgatgggaa aagatagaac
    36721 agaaaggatt ttaccagcat gattacaaag gcggacatca aacaggagac gaattccgta
    36781 tcatataacc gtggaaaaaa gatttatgaa gaacagaaag tgcatgcttt tcaggtacag
    36841 gaaatggagg atattttcgg atatcaactg cataaaatta cagctgtcgt agacggaagc
    36901 ggaaaaaata tgtattgtgt cagcgtttct gtagatgaag aaatgtctga aattatggaa
    36961 gatgattgtg attgtccggc acatgagcag tactggggat tgtgcaaaca ttgtgtcgct
    37021 gttttgctgt actatcttga atggcggaaa aaagagagaa aaaaactgga agaaaaagtg
    37081 agaaatgacg aagaacatca ggaactggag cagcttcttc gtgctgtcgg cgtgaaaaaa
    37141 ggaatggaag aattccaggg agaacataaa attgtcagaa aagtagtaaa accaacacca
    37201 aaagcagaga caagccccgg tttttctgaa ctgctttcac attatgtgat gcgggatcag
    37261 gttgcttttc tgccgaaagc aaaagcaggt caggttaaac tggaaattca tctggagagc
    37321 cagtttgacg attcctttcg ggcagaattt aaaatcggaa gcaaaaggat gtatgtatta
    37381 aaaagtgttt ccaaaatgac agcagctgta aaaaatatgg aaacggtcag ctacggtgcg
    37441 cagctttctt ttttgcactg tatggatgct tttgaagaag aaagccgtcc gatggtagaa
    37501 tttctgagcg catataccat ggaacgcggc tcatgctatc agataggtac cggcagctat
    37561 gcgtcctgct ttgatgaccg ttatgtgatg atttctgtgc agaatatgga tgaatttttt
    37621 gaggctgttg gcagccagga gttcttttgt cgtacgaact ggagagaaga aaaactgtgg
    37681 cactgccagg aggggatgcc gcagtatgaa atgaagcttt tacggaaagc agatggccta
    37741 aaggtacaga tgtacgcgga tcccattttt tatggcagaa aatatttgta ttttctttca
    37801 gaaaaaaata caatttaccg aatcccaagg gaagagatag cagaagtctg cgaattcctg
    37861 gaatatatgg agcgatgtcc ggacggagtc tgtgaaattg caaaagaaga tatcccgacc
    37921 ttctgccagg gacttcttcc ggtgttggag gaacatttta aggtaaaaaa agaagaaaag
    37981 ctggaattac agcagtatct tccgccgcag gtggaatttc agatttatct ggatgcgccg
    38041 cagcatgata tgattacctg cgaacttctg gctgtctatg gcgagaaaaa atacaatgtt
    38101 tttgcagatg caaacgatat ccatcagctt tcccatggcc gtgatgtacg gaaagaagca
    38161 gcggcaaacc agctcgttcg atcctgtttc agcgcctatg atgcgcggaa acatcagatg
    38221 cttctccagg gagccgatga gatgtatgag tttttaagca gcggtatgga agatctgcag
    38281 aaactgggcg aaatttttgt ttctgaccgg ttaaaagcga tccgtgtcat cccgtccccg
    38341 aaagtttctg tcggtgtatc cctcgcagaa aatgtactgg aacttcattt gaatccgggc
    38401 aattttggca tggaggaact ggcagagatt ctttcaaaat atgaccggaa aaagaaattt
    38461 taccgcctga ggaccggtga atttatggat atggacgagg acggtatccg cgttctctcg
    38521 gaactgcggg aaaatctgca gatctccgag gcaaagctga agagcggcga gatcacaatt
    38581 ccgaaatacc gagcactcta cctcgatacc cgtttaaaag agcaggatga tcttcaggtt
    38641 gagaaaaacc gcgagttccg tatgctgatc cgaaatatga aaactgcaga ggagaacgat
    38701 tttgaacttc cggacaatct gcaggcacag ctccgtgaat atcagaaaac aggattctgg
    38761 tggctgaaaa ccttatgcca gaacggcttt ggcggtattc tggcggatga tatgggactt
    38821 ggaaagacgc tgcagaccat cgcctttctc ttatctgaaa tgcaggaagc acccgaagat
    38881 gcaaatcgtc gttctttgat cgttgcaccg gcatctctcg tctataactg ggagagcgaa
    38941 tgtgcccgct ttgctccgga gctgcagacg agactcgtcg ccggcacaca ggaacagagg
    39001 aaagagatga tccaaaatgc cggtatgcag gatattttga ttacctcgta cgatttgctc
    39061 cgccgcgata tcgaactgta tcaggatctc ccgttcttct gcgagatcat tgatgaggcg
    39121 cagttcatca aaaatcacgc aacgcaggga gcaaaagccg tgaaaacgat ccatgcatcg
    39181 ttccgcctgg cattaaccgg aacaccggta gaaaaccagt taagtgaact atggagcatc
    39241 tttgattatc tgatgccggg attcctctat ggatatcaga aattccgcga gcagtttgaa
    39301 cttccgattg tgcggaacgg cgacgaagaa agattagaac gtctgcaaaa gatgatccgt
    39361 ccgtttattc tgcgccgtct gaagaaggaa gtgctgaagg atctgccgga caaaattgag
    39421 aaaaatatgg ctgcctgcat ggaacagaag cagaaggagc tgtatcatgc acatgcccag
    39481 cggctggcgc ttatgctgca gaaccagaca gaggaagaat ttgccgattc caggttccag
    39541 gtgctctctg aactgacgcg gctgcgtcag ctctgctgtg atcctgcgct tgtttacgaa
    39601 aattacgaag gcggttcggc aaaactcgat ctctgcatcg agctgattca gagtgccgtg
    39661 gaatccggcc acaaaatcct gctgttctcc cagttcactt cgatgatcga gatcctgacc
    39721 gcgcgtctgc aggaagaaag catcagctgc tttgtcctgc agggaagcac gaaaaaagag
    39781 cagcgtgcac aaatggtgga acagttcaac cgggatgaca cgtctgtatt ctgtatttcc
    39841 ctgaaagcag gtggaaccgg cctgaacctg accggcgcgg atatcgtcat ccattacgat
    39901 ccatggtgga atgttgctgt tgaaaatcag gcgacagacc gtgcacatcg tattgggcag
    39961 aaaaatgttg tgacggtata caaattgatt gcaaaaggaa ccatcgagga aaaaatcatc
    40021 aagcttcagc agatgaaaaa agagctggcg gaagagatcc tttccggtga tgaaattaag
    40081 acagcatcct ttcagagaga ggaactgctt gagcttttgg aagaacggtt ctgaaaccgc
    40141 gaaaagcaga agaagatttg atgtaaggac gaaatgcagc agcaatgcag gaaaatataa
    40201 aagggatacg tttctgtcat accctgaaaa cgggcggaca ggaaacgtat cccttttaca
    40261 gtaatagtct attcgaaacc aatcttacag aagctgaata ttcatcacat ccatcacctc
    40321 tcgtgtctcc atcggccata ccgagcactg tacctctccg atgtgcgctt tgcgcaggaa
    40381 aaacatacag atacgggact gtccgatacc gccgccgatt gtctgcggga gagtgccgtt
    40441 cagaatggat ttctggaagg gaagcttcgc gcgggactgg catccgcatt cctttaactg
    40501 agcgcggaga gcatctgcgt tgacgcggat acccatggag gagagctcaa gagcgatgtc
    40561 aagcactgga taataaacga tcaggtcgcc gtttaaggac cagtcgtcgt agtccgggga
    40621 acgtccgtcc tgacgctctc cggaagcaag cacgccgccg atctgcataa taaagacagc
    40681 acctttttct ttggtgattg tatactctct ttcctttgga gtcagattcg gatagcgatc
    40741 ctcaagttcc tgggaagtga taaagaaaat ttcatccggc agaatggttt cgatgtagtc
    40801 gtactggtct gccatatgtt tttccgtgtt tttcagtgcc tcatagatat cttttacggt
    40861 actctttaag aaatcaagat tgcgttcttc cggggacatg attttttccc agtcccactg
    40921 atccacgtaa acggagtgaa tgttgtcggt cacctcgtcc tggcggatgg cgttcatatc
    40981 ggtgtagatg ccttctccct cagagaagcc gtatttgtga agtgcgtacc gtttccattt
    41041 tgccagagac tgaacaattt cagcggtttt cttggtaccc tgatccttta actcaaaaga
    41101 aaccgggtgt tctacgccat tcagattgtc attcaagccg gtttccggct gtacaaaaag
    41161 tggagctgtt acacggagca gatgcagacg atgtgtcagc tcatgctgga aataatcctt
    41221 tactgtttta atcgccacct gtgtatcgtg gaggtttaat gcagatttgt aaccctgcgg
    41281 aataataatt tctcccatta ctttaaaacc ctcttgtttt agatttgctt ttcgaatcat
    41341 gccttctcaa aaagcttcaa atttagttta ccatgttttc cctggaaagc aaggaaaaga
    41401 tacgaaaaaa ccaaagatag taaagaattt tttcgacata ttttccttgc tgaatttctc
    41461 cccggaaata cgaaattttc cgaaaatcag agcgcttatg aaagaggcgg aattattcgg
    41521 aaacccactg gcgcagaaac gctgcggact gttcgatgaa cgataactga tccggcgcat
    41581 cgaaatgctc gatggcgaga taaccgtcgt aatcgcacgc ttctaaatct gtaagaagct
    41641 ttgccatcgg cagatatccc gttccggccg gtgtctggcc aaggccgcgg cagaaacgtc
    41701 cgtgaacgag cggactttcg gcacgatcct tacagtgaac atggacaata taatccttca
    41761 gaagccaggc agcatacgaa acatcctcat cactgaaagc aaaatttccg atatcaagcg
    41821 tataccgcag ccccggcacg tgctccataa accagagcag cggatatgtg cgtgcaaacg
    41881 gctgtaaaaa accgtcaaag tcctctaagg taatcgtaac gccacgcaaa gccgcatatt
    41941 cggcgagtga ggaaagcgca tgtaccatat tttgaatcga tttgttttcg gccataaaag
    42001 tactcgttgc ctcataggtg ctgctgcagg ctgcaagctc tgccgcttcg gcagtttcaa
    42061 gcgcgcctgc aacaaacagg acacgcgaaa cctgctgcga tgccgcagta tcgagcatct
    42121 gctgtgctct gccaagatcc gtatcatggg aaaaatcaaa ggtttcatgc atacagctga
    42181 tcaccagccc cgcatcggaa agcatcgggg caatgacctc tctttctgat gcaaacagac
    42241 tgtagttcat ctcaacagcc gaaattccct gctccctgca gaacgcgagc acttccggaa
    42301 tcgtcttacc ggactgctca cacgcctgta gaatgtgttc gtaaaaaacg gaaagctttc
    42361 tcatataaca tttcctcctg atttatgcaa tgtgtacgca gattccatcc gtgtattgca
    42421 gaacattttt gatcgtgaaa tcagacccct gttttgaaaa aatcaaacgg gatacagata
    42481 tcggtaaact gcagctgcta tttttggtgt gaagaaagat acacttcctc cgtagatgga
    42541 ttcacccatg cattatccag tggtgccggt cttcgtacta caggaacgca ccagcgcacc
    42601 ccatttttga taatcttctg aatcaccggc atatggtaaa tggggtattc ctcgtgtccc
    42661 ggctgaaaat agaaaatttt gcccagccca cgtgtaaagg tgcatccgct gcggaacacc
    42721 tcaccgcctg caaaccatcc cgtaaatacc acatcatccg gtttgggaat atcaaaaaac
    42781 tccccataca tctcttcctc cggaatatcc acccatgccg gaattccctg cgcgatcgga
    42841 tgggtcggac acgtacagaa cagtctctca cgatccccat gccgccaccg aagcgtcata
    42901 cttgttccga gaagcttccg cataatcttg gaataatgcc ctgaatgcag aactaccagc
    42961 cccattccgg ccagcacatg acgccgaacc cgctcggcca catcgtccga gaactcctcc
    43021 tgcagcatat gattccagaa caccagcaca tcggtatttg ccagcacttc ttccgtcagc
    43081 ccatgctcct tctcgtcaaa cacagccgtc cgaaccgaaa gctcctcctc ctgtcccaga
    43141 aactttgcaa tacaggtgtg aatcccgtcc ggataaatct ctttcacttt ctcctgcgtt
    43201 cgctcatgct gaaactcatt ccagaccgta actcgtatca ttcgtccatc ctccttctat
    43261 aagaaaaaaa tgtatcattt tcaaaaagtt atcgatagca aatcttgaaa ataatgaaaa
    43321 aagcagtcgt tcacactctg cggtactgaa tttttgcgtt attttctggc cttactataa
    43381 attatactcg tgctatacca atatttcaat tcacaaattc cttgacatcc cctccgaaat
    43441 cccgtatact aaacaatgcg cagccgggga gatagcggtg ccctgtaccc gcaatccgct
    43501 acagcggggg tgatgccaat ccgagggctc tttcattgcg aagctgcctt ctataagtga
    43561 cgttgacgtc tgggtcttac gcaacaggac tccatgaacc gtgtcagggc aggaatgcag
    43621 cagcactaag tggaatctcc tgtgtgccgt gagggtgcct gggccgagta ggctgtagga
    43681 gtaacgttcg tggtgagagt tcgaagtgcg gcgcgcataa aaaagaaaat ccaaggaagc
    43741 ctgaaccaca gacttccttt tttcgtacaa aaaatagaaa ctcccgcgct ttcttccccc
    43801 gaactattgt gagaacgaaa aaaatagggt ataatgtaaa cattccataa tactgccaag
    43861 aaacaagaag cagtcgtgaa aatctaataa agaacaggat gggaaaagtc atatgttaag
    43921 aattggtctg ttaacaagcg gcggagactg ccaggcatta aatgcgacca tgcgcggagt
    43981 cgtaaaaggc cttgcaaaca atgtaaaaga tctcgaagta tacggattca tgaacggcta
    44041 taaaggcctc atctacagcg attaccgcat gctcagtgca aaagattttt ccggtatatt
    44101 aacacaggga ggaactattc tcggaacctc acgtcagccg ttcaagctga tgcgtacccc
    44161 ggatgcaaac ggtctggaca aagtggaagc aatgaagcat acctaccaca agctccttct
    44221 ggactgcctt gtcattcttg gcggaaacgg aacccagaaa acagcaaacc tcttgagaga
    44281 ggaaggactg aatattatcc atcttccgaa aaccatcgat aatgatatct acggaacgga
    44341 tatgaccttc ggtttctaca gtgccgtcaa tattgcatcc gatgcgatcg actgcatcca
    44401 tacaacagcc tcctctcacc agagagtttt tatcgtagag gttatgggtc ataaagtagg
    44461 ctggctgacg ctgtacgccg gaatcgcaag cggcgcagac gttatcctga ttccggaaat
    44521 tccatacgat attgaaaaag tatgcgaggc catcaacaga cgaaaagagc gcggaagcgg
    44581 attcaccatt cttgccgttg ccgagggtgc aattgcaaaa gaagacgcag cacttccgaa
    44641 gaaagagctg aaaaaacgtc aggaagaaat cgcaaagaaa tacccgtccg tatcctacaa
    44701 gatcgcagaa gagatcgagg caaagacagg catggaaatc cgcgtcaccg taccgggcca
    44761 catgcagcga ggcggcagcc cgtgtccgta tgaccgtgtc ctttccacaa gactcggatc
    44821 cgaggcagca cagcttattc tggacaaaca gtacggctac atggtcggaa tggtcaacgg
    44881 aaaagtaaag aaaattccgc tgggcgagtg cgccggaaaa ttaaagaccg tagatccgaa
    44941 tgcacaggaa gtcctgcagg caaaacggat gggaatcagc ttcggcgatt gatgcgttcc
    45001 cgaaaaaggc aaggaaaaag aacggaggcc ttaaagcaag aagaacaagg tctccgttct
    45061 tctgttgcac tgctgtccaa aagaaactgc cggcggcagg cttccatgcc gatgcaataa
    45121 aaggagaaaa attttatgag ctatactgct ctgtaccgaa aattccgtcc ttccaccttt
    45181 gaggatgtaa aaggacagga tcacattgta acaacattaa aaaatcagat ccgcgcggac
    45241 cgcatcggtc atgcgtacct gttctgcgga acccgcggaa caggaaaaac gacgatcgcc
    45301 aagattctcg cccgctcggt caactgcgaa catcccgtgg acggaagccc gtgcaacgaa
    45361 tgtccgacgt gtaaggcgat tctggcgggc gtttccacga acgtcatcga gatcgacgcg
    45421 gcatcgaaca acggtgtcga caacatccgt gaaatccgtg aggaggtagc ctaccggccg
    45481 acacagggac ggtataaagt ctatatcatc gacgaggtgc atatgctttc taccggagcg
    45541 ttcaatgcac tcttaaaaac cctggaggag ccgccgtcct atgtcatctt cattctggcg
    45601 accacagagg caaacaaaat cccggtgacg atcctttcca gatgccagcg gtacgatttc
    45661 cgccgcatca cgatcgacac cattgcggcg cgtcttagtg aactgatgga aaaagagggc
    45721 gtggacgtcg aagaaaaagc catccgctac gtcgcaaagg cgggcgacgg ctccatgcga
    45781 gacgcgttaa gcctcctgga ccagtgcatc gccttctacc tcgggcagaa gctgacgtac
    45841 gataaagtcc ttgaagttct cggaactgtt gataccgaaa tcttcagcca gatgctccgc
    45901 acgatcctcg ccggggatgt aacgggaagc atccgcacgc tggaaaccct tttaaaccgc
    45961 ggaaaagagc tgggacagtt cgttaccgac tttacgtggt atctgcggaa tctgctgttg
    46021 gtaaaaagcg cagatgaaac cgaagaactg ctcgacgtct ctacagaaaa cctgaagctc
    46081 ttaaaagaag agagcacgat ggtagacgat gagacgctga tccactatat ccgcgtttta
    46141 tccgagctct ccggccagct ccgctatgcg gcgcagaagc gcgtgctcgt tgaggtggcg
    46201 ctcgtcaaac tctgcaaacc gcagatggag cagaacctcg aatccgtgct ggagcgtctt
    46261 aggattctcg aagaaaaaat ggagcgcgga atcccgatga tggtgacaaa tgcaccggga
    46321 gcaggaaacg gcagtgcagg aggctccgga gcggccggag acggctatgc ggcggcagaa
    46381 acaccgcaga gtgcaccggc accagtgaaa gccgcaccgg cggatttaca gcgcatcagc
    46441 gcgtcctgga aggcaattgt cggccagaca agcggcatgt tccgccagtt tctgcagagc
    46501 gccgttccga aatacaacag cgagacgggc gacgccaaac tgtttgttga gtttgcagac
    46561 cagctcgcat ccatctacgt tgagcggccg gaggcagccg atgagatccg caccattatt
    46621 gaacagatga ctggaaaaac cgtagaggtc cagctcattt tagcagacca gcatgcaaac
    46681 gccgcgctct ccacgatttc cgtggatgat gcactgacgc aggcgattca tggaatcccg
    46741 gtcgaggagg acgacgagag cgacttcgac gacggggaag aataaattaa aaacaaattt
    46801 ttcaggagga tattaccatg gcaaaaagag gaggattccc gggaggaatg ccgggcaaca
    46861 tgaacaatct gatgaagcag gctcagaaaa tgcagaagca gatggaagag aaccagaaag
    46921 cactggagga gaaagagttc actgcgacag caggcggcgg agccgtagaa gtaaccattt
    46981 ccggtaaaaa agaagtaacc aaagttaaaa tcagcgaaga tgccgtagat ccggacgacg
    47041 tagaaatgct cgaggatctg atcatggcag caaccaacga agcactgcgc aaagttgagg
    47101 acgaggcagc caaagctatg ggcaaacttg ccggcggact cggcagcatg ggcggaggat
    47161 tcccgttctg atggattact acagcggata catcaatcag ttgatcgagc agctgtccca
    47221 tctgcctggc atcggtgtga aaaccgcaca gcggctggcc tttcatatta tgaatatgcc
    47281 ggccgaccag gcaaaacggc tggccgattc gatcacagaa gcaaaatccc acgtccagta
    47341 ctgcaagtgc tgccagaccc tgaccgacaa agagctgtgc ccgatctgcg caaatgtcaa
    47401 gcgcgaccac agcgtgatca tggtggtgga gacgacgcag gatcttgcgg catatgaaaa
    47461 aaccggaaaa tacgacggcg tttaccacgt cctgcacggg gccatctccc cgatgctggg
    47521 gattgggccg gacgacatcc ggttaaaaga gctgatgcag cgcctccagg gcgatgtctc
    47581 ggaggttatc gttgccacga actccagcct ggagggcgag acaacggcca tgtacatcag
    47641 caagctgatc aagccgaccg gcatcaaggt gacgcggatt gcaagcggtg ttccggtggg
    47701 cggcgatctg gaaaatattg acgaagtcac ccttctgcgt gcgttagagg gcagaacgga
    47761 gctgtaatta tgggcagaaa aaaagtgaca tccagtgatg tcgcagcagc ctcaggcgta
    47821 tcccaggcga cggtctccat gatcctgaac cggcgggaaa acgtttcgtt ctcgcgggaa
    47881 acagtagaaa aagtggaggc ggcggcaaaa gagctgggct accagattcc gcggcggaag
    47941 atgagaagag agtcgaacgg caacaagctg atcgtcgtct tctgtccgac gctgacgaat
    48001 ccttactatg tgatgctgct gcacggcatc gaagaagttg caaaagagaa gaattacggg
    48061 gtctttgtct gcaatacgca gagggatctg aaaatcgaag agaattatct tcggatgatg
    48121 aagagtgtgc gcccgcgcgg cattatctac acctgcaatc cgagcagaag ctgcaaggaa
    48181 aaagtggagg agctggccgc ggagattccg cttgtcatca tcaacaaccg agaagagctg
    48241 ctggaaattg atgcggtcga gctgaacaat gcaaaacccg gcaggctgat ggcacgccac
    48301 ctgctggaac tgggacaccg gcatgtggcg tttgtttcac ctccgctgac ttccaaacag
    48361 ggccagcgcc tgaaacgcgt ggagggcttt gtgcgcgagt ttgaggaggc aggatacggc
    48421 gacggcgtga tcgtcaaatc ggcggatgag gacctcgacg aggtgatccc gagcatggat
    48481 tctgagtaca agatgggcta ttatctgacg aaggaactgc tgcgggagga caagaacctt
    48541 acggcaatcg tcggcatgaa tgatatgatc gcgttcggga tcatggacgc cgtgctcgag
    48601 agcaaactgc gcattccggc ggatatttcc gtagtgggat gtgacaacac cgtctattcc
    48661 agcttccgct cgatttccct gacgacgatc gaccactatg tgccattgaa gggccgcgat
    48721 gcgtgcgata tcatcctgcg gaaaatccag tctcttcggg aaagccagga cggcaatgaa
    48781 cccttaagca cgtatcatgt cgagtatgag ccgaaactga tcgtgcggcg cagcacttcg
    48841 tatgcaagaa ccgaaaaatt aaaaaataaa taggacggct gaaattatta ttaataattt
    48901 tgaataaaac agctccggcg caaaccggga aaacacaaag aaaaggaaaa accggaaaaa
    48961 atgagaaaaa atccgccaga gtaatttata aaatagtatt tataaataat aaaattaatt
    49021 aataaaaatg aggccgactt gacggaaaaa ccaaacatat ctatactgga gataacagaa
    49081 aaacggagcc gttctgcgga aaacaacagg ccccgaaacc ttacgaaagt atgaatatgg
    49141 gaggaaaaac aacatgagat ttttcatcga cacagcaaaa gtagaggaca ttaaaaaagc
    49201 aaacgacatg ggcgttatct gcggcgttac caccaacccg tccctgatcg caaaagaagg
    49261 acgtgacttc aaagaggtca tcaaagagat caccgagatc gtagacggac cgatcagcgg
    49321 cgaggtaaaa gctaccacaa cagatgcaga aggcatgatc aaagaaggcc gtgagatcgc
    49381 agccatccat ccaaacatgg ttgttaaaat cccgatgacc gtagaaggac tcaaagctgt
    49441 taaagttctg agcgcagagg gcatcaagac aaacgtaacc ctgatcttct ccgcaaacca
    49501 ggcacttctg gctgcaagag caggtgcaac ttatgtttcc ccattccttg gaagactcga
    49561 tgacatcagc acccgcggcg ttgatctgat ccgtgaaatc gttgagatct tcgacgtagc
    49621 cggcatcgac accgagatca tcgcagcaag tgtccgcaac ccgatccacg taaccgactg
    49681 cgcactggct ggtgcagaca ttgcaactgt tccatacaac gtaatcgtac agatgaccaa
    49741 acatcctctg accgacgccg gaatcgcaaa attccaggct gactacaaag ctgtattcgg
    49801 cgaataagac ctaaacatga ttgcataaaa gctggaaata tggtattgtg aacaaagtga
    49861 acaatagcga tatttccagc ttttgattcc gattcaacta aacaaggaga aaagacatga
    49921 acaagttaga acttcagaag acggccaatg agatccgcaa agggatcgtc agcggcgttc
    49981 atgcagcaaa ggccggccat ccgggcggat ctctgtctgc agccgatatt ttcacgtatc
    50041 tgtattttga agaaatgaac gtggacccga aaaatccaaa agatccggac cgcgatcgtt
    50101 ttgtactttc gaaaggccac acagcgccgg gactctacgc agcactggct gaaaaaggtt
    50161 acttcccgaa agaagacctg ctgacccttc gccaccttgg ctcttacctg cagggacatc
    50221 cggatatgaa acatatcccg ggcgttgata tgtcaagcgg tt
//
LOCUS       DAOMUF010000003        39748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_84_length_39748_cov_3.561610, whole genome shotgun sequence.
ACCESSION   DAOMUF010000003 DAOMUF010000000
VERSION     DAOMUF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 39748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39748
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84_length_39748_cov_3.561610"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1855
                     /gene="rho"
                     /locus_tag="PLN77_00475"
     CDS             191..1855
                     /gene="rho"
                     /locus_tag="PLN77_00475"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRM42785.1"
                     /translation="MTREKYESLPLSTLKALAKSRNMKGISTLRKEALIERMLEEDQK
                     ENQKNMEEPAVLKEEKKTETVQDQNQEPQEKREDGMERQNRETSENGTARPYQRPRYV
                     RREERSENGERQEYRPRAPRFNERRFQETRNYNQNNREEEQRQEPRQEAGQEMRQEPL
                     RQEARPETRQEPRQETSTLDSGQEACGILEVMPDGFGFIRSDNYMPGDSDVYVSPSQI
                     RRFGLKTGDIIRGNTRVKSATEKFSALLYLKSVNNLPLDQIMRRGNFEDMTPVFPDER
                     LRLERPGGSMAMRIVDLVSPIGKGQRGMIVSPPKAGKTTLLKDAAKSILRNNPEMYLI
                     ILLIDERPEEVTDIKEAIQGSNVEVIYSTFDEQPEHHKRVSEMVIERAKRLVESKKDV
                     TIFIDSITRLARAYNLTVPPSGRTLSGGLDPAALYMPKRFFGAARNMREGGSLTILAT
                     ALVDTGSKMDDVVYEEFKGTGNMELVLDRKLQERRVFPAIDIAKSGTRREDLLLTPDE
                     QEAVYNMRKALNGMKSEEAVENILNMFARTRNNGELIQILKKQKIV"
     gene            1987..2187
                     /gene="rpmE"
                     /locus_tag="PLN77_00480"
     CDS             1987..2187
                     /gene="rpmE"
                     /locus_tag="PLN77_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRM42786.1"
                     /translation="MREGIHPEYHQATVTCNCGNTFVTGSTKKEIHVEICSKCHPFYT
                     GQQKAAAARGRIEKFNKKYGMK"
     gene            2292..>3109
                     /locus_tag="PLN77_00485"
     CDS             2292..>3109
                     /locus_tag="PLN77_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRM42787.1"
                     /translation="MKSSNIGGQAVMEGVMMMNKDRYSVAVRRPDGEIEVKVEQHKPL
                     TKHKGLLKVPILRGVFSFADSMIVGMSSLMYSASFYEDEEEEKKKPATKEEEEQQKAK
                     KEKTDRLMMYGTVTFSVVIAVVLFLMVPYFLSELLHKAGAGTTLTAVAEAFARVALFL
                     GYLAAISRMEDIQRVFMYHGAEHKCINCVEHGLELNVENVLKSSREHKRCGTSFLMYV
                     IVISIIFFMFIRVDSPVLKIVIRLLLIPVIAGVSYEFIRLAGTSENKIVGLLSKP"
     assembly_gap    3110..3119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3120..3345
                     /locus_tag="PLN77_00490"
     CDS             <3120..3345
                     /locus_tag="PLN77_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRM42788.1"
                     /translation="EIIRLAGKSDNKLVNLISKPGLMLQHLTTREPDAEMAEVAIAAV
                     EAVFDWRAYLNENFGYTYPAKTEKKEHEEP"
     gene            3362..4198
                     /gene="prmC"
                     /locus_tag="PLN77_00495"
     CDS             3362..4198
                     /gene="prmC"
                     /locus_tag="PLN77_00495"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRM42789.1"
                     /translation="MEKTALESAILYLQKQGVEEARQDAWLLLEHVCGISHSTYFVHS
                     EDEMPKEQQEQYEALVRKRGEHIPLQQLTGEAWFYGIPFFVNEHVLIPRQDTEILVEE
                     ALRGLPKDARILDLCTGSGCILISLLVTREDCTGIGADLSEKALQVAEKNGAQAGGRA
                     SFVKSNLFENIEGTFDVIASNPPYIRTDVIETLMPEVRDHEPRMALDGSADGLVFYRK
                     ITEQAGSYLKEGGKLCFEIGYDQGDEVRELMETHGFADVQVLKDLAGLDRVVTGIWHK
                     KP"
     gene            4274..5350
                     /gene="prfA"
                     /locus_tag="PLN77_00500"
     CDS             4274..5350
                     /gene="prfA"
                     /locus_tag="PLN77_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRM42790.1"
                     /translation="MFDKLDDILMRLEEVLNQLSEPDVAADAAKFQKLMKEQAELQPI
                     ADAYKDYKTQKQTIEESLMLLEEESDEEMREMLKEELSDAKKRVEELEQELKVLLLPK
                     DPNDDKNVIVEFRAGAGGDEAALFTAEICRMYIKYAESRGWKTELTSADENGIGGFKD
                     ATLLINGAGAYSRMKYESGTHRVQRVPETESGGRIHTSTMTVAIMPEVDDVEVQIDPK
                     DYKFDVFRASGNGGQCVNTTDSAVRLTHFPSGIVISCQDEKSQLKNKDKALKILRARL
                     YDLEQQKQHDEMAELRKSQVGTGDRSEKIRTYNFPQGRVTDHRIKLTLYKLDAIMNGD
                     LDEIIDSLIAADQAAKLSSMQEEA"
     gene            5443..6363
                     /locus_tag="PLN77_00505"
     CDS             5443..6363
                     /locus_tag="PLN77_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRM42791.1"
                     /translation="MKKTALVIMAAGIGSRFGKGIKQLEPVGPNGEIIMDYSIHDALE
                     AGFNKVVFIIRKDLEKDFKEVIGNRISKITEVEYAFQELDDLPAGFAKPEGRTKPWGT
                     GQAVLACKGLVKEPFAVINADDYYGKDPFVKLHDYLVQEHDVDEVMPISMAGFVLGNT
                     LSDNGGVTRGVCVVDENGNLTGVNETKNIVKTAEGAGVEKEDGTVDPIDKNSLVSMNM
                     WGLQPEFIDLLEKGFVEFLSAQKEGDVKGEYLLPIYIDELLHAGKAEVKVLPTDAEWF
                     GVTYQEDKASVTEAFRKLIADGVYSEKLYD"
     gene            6468..7814
                     /gene="glmM"
                     /locus_tag="PLN77_00510"
     CDS             6468..7814
                     /gene="glmM"
                     /locus_tag="PLN77_00510"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRM42792.1"
                     /translation="MGKYFGTDGFRGEANIDLTVEHAYKVGRFLGWYFGKDHKAQIVI
                     GKDTRRSSYMFEYSLVAGLTASGADAYLLHVTTTPSVSYVVRTENFDCGIMISASHNP
                     FYDNGIKVINGLGQKLEADIEAQIEAYIDDGTPKIPFATREDIGRTVDFAAGRNRYIG
                     HLISIPTKSFKNMRVGLDCSNGSASSVAKSVFDAIGAKTYVIHNEPDGTNINCGCGST
                     HIEELQKYVREKHLDVGFAYDGDADRCIAVDELGNVVDGDLIMYVCGKYLKEKGQLNG
                     DTIVTTIMSNLGLYKACDRAGIRYEKTAVGDKYVCENMMANGYSLGGEQSGHIIFSKH
                     ATTGDGILTSLMVMEVLIDKKLPLSTLTAEVVIYPQLLKNVRVANKAAAKANLAVQKA
                     VEDVAEALGDDGRILVRESGTEPVIRVMVEAPDDATCAKYVDQVVSVMHKEGLVTE"
     gene            7811..8845
                     /locus_tag="PLN77_00515"
     CDS             7811..8845
                     /locus_tag="PLN77_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM42793.1"
                     /translation="MKKRLVIFFLAAAMVLGGCENKNSKLYKKGIEALEQKDYVTSIA
                     MLEGAVKAGDRLAESYRSLGIAYLKSQEYDKAKEAFKSSLSSMKHKDAEFSRDVMYYE
                     AETCVQAGDLDGAIEICTNIQEEKADADALFLRGRAYFLQKNYEQAKVDFDAAVETKE
                     SYQLCLDIYELYQESSMKADGDRYLEAAVKIEPKTTEDYYNIGCAYYYLEEQDEAVKA
                     FSKAMKDGSSAAMEMLGEVYLSNGQNDEAKALFSSYLSTDGFAAAANNGLALCALAEN
                     DTDSALSYIEEGLKTAEDSDRENLLFNQIVSYEKRADFDTAKSLMADFLAAYPENTAA
                     QRENTFLQNR"
     gene            8864..>10079
                     /gene="hflX"
                     /locus_tag="PLN77_00520"
     CDS             8864..>10079
                     /gene="hflX"
                     /locus_tag="PLN77_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRM42794.1"
                     /translation="MFDLREEQERVILVGVQENGGANAEESLDELAELASTAGAKVEG
                     RLVQVREAIHPGTYIGKGKLEELKILVRACDATGIICELLYDLDATGVICDDELSSIQ
                     LKGLEEALECKIMDRTLLILDIFAARAVSSEGKIQVELAQLKYRASRLSGLGTSLSRL
                     GGGIGTRGPGEKKLETDRRLIRTRITQLKRELEEVRQHRELLREGRKRSKIPVAALVG
                     YTSAGKSSLLNALTGSEILADAMLFSTLDTTTRSLTLPDGQEILLTDTVGFINKLPHH
                     LIDAFRSTLEEARYADYILHVVDTSNPQMELQMQVVYDTLRELKVEDKKIVTLLNKQD
                     KLLAPMVVKDFRADASLAVSAKTGQGLEDLKNLLSSWMMEGKIYIERLYPYDKAGTIS
                     LIRGYGILLEEEY"
     assembly_gap    10080..10089
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10395..12806
                     /locus_tag="PLN77_00525"
     CDS             10395..12806
                     /locus_tag="PLN77_00525"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HRM42795.1"
                     /translation="MFQVEKRDGTIAEFQMKKITDAIGKAFGAKDMQFSDDMLQMLAL
                     RVTADFQSKIKDGKISVEAIQDSVENVLIQCGYAEVAKAYILYRKQREKIRNMKSTIV
                     DYKEIVDSYVKVEDWRVKENSTVTYSVGGLILSNSGAITANYWLSEIYDDEIAEAHRN
                     ADIHIHDLSMLTGYCAGWSLKQLIKEGLGGINGKITSSPARHLSVLCNQMVNFLGIMQ
                     NEWAGAQAFSSFDTYLAPFVKVDNLSYGEVKKCIEAFIYGVNTPSRWGSQAPFSNITL
                     DWTVPDDLAELPAIVGGKEMDFKYKDCKKEMDMVNKAFIETMIEGDANGRGFQYPIPT
                     YSITSDFDWSDTENNRLLFEMTAKYGTPYFSNYINSDMKPSDVRSMCCRLRLDLRELR
                     KKTGGFFGSGESTGSVGVVTINMPRIAYQSKDEKEFYRRLDHMMDIAARSLKTKREVI
                     TRLLDEGLYPYTKRYLGTFENHFSTIGLIGMNEAGLNAAWLGKGLEDPKTQQFTKEVL
                     NHMRERLSDYQEEYGDLYNLEATPAESTAYRLAKHDRAKWPDIKTAGHEGDTPYYTNS
                     SHLPVDYTEDIFSALDIQDDLQTLYTSGTVFHAFLGEKLPDWKAAASLVRKIAENYKL
                     PYYTLSPTYSVCANDGYLAGEHFTCPICGKEAEVYSRITGYYRPVKNWNDGKRQEYKN
                     RTVYDIIHSKSPEQKMKSYGAAEKLAEQAAGKEEPKAAAAADKIEEDGMYLFTTSTCP
                     NCKMAKEMLAEEQYEVIDAERHPDLAAQYGIMQAPTLLVVEDGQAQKIVNASNIQKYV
                     DEYLS"
     gene            12965..14242
                     /locus_tag="PLN77_00530"
     CDS             12965..14242
                     /locus_tag="PLN77_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRM42796.1"
                     /translation="MGYSQDTICVQGGWQPKNGEPRVLPIYQSTTFKYATSEEMGKLF
                     DLEEEGYFYSRLQNPTNDVVAQKICDLEGGVAAMLTSSGQAATMLAVTNICEAGDHVV
                     CAAKVYGGTSNLLAVTLKRFGIETTLVDQDAPAEELEKAFQPNTKAVFAETISNPAGV
                     VLDIDKFVKLAHEHGVPMICDNTFATPINCRPFEFGVDIVTHSTTKYMDGHAMALGGA
                     IVDSGNFDWDAHADKFPGLTTPDESYHGVIYTQKFGKKAYITKATAQLMRDMGACQSP
                     QNAFLTNVGLETLHLRVERHCRNAEKVAEFLKNHPKVAWVEYAGLADSKYHALAEKYM
                     PNGTCGVLCFGPKGGRDGAMRFTNGLKMVAIVTHVADARSCVLHPASHTHRQLNDQQL
                     IEAGVLPDLIRLSVGIEDVNDIIADLDQALANV"
     gene            14284..14766
                     /locus_tag="PLN77_00535"
     CDS             14284..14766
                     /locus_tag="PLN77_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRM42797.1"
                     /translation="MEEMAKKRVKDSETEQSYLLMHRHINGYGRLFGGQLVAWIDELA
                     GLVGRRHCGHEITTACIDNLNFKHPAFLNDTVVLVGRVTYVGRTSMEVRVDTYVEELA
                     TGIRKMINRAYVVMVAIDENGRALPVPGLILESPEQEAEWEGGKRRYELRKERHREGF
                     "
     gene            14919..15122
                     /locus_tag="PLN77_00540"
     CDS             14919..15122
                     /locus_tag="PLN77_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRM42798.1"
                     /translation="MDYLEAAYVLKLRRGYVRNAMICRYMGYSQASISAGIKMLREDG
                     CLVRDSDGFLDITEKGETLAKKI"
     regulatory      15187..15267
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            15346..16059
                     /locus_tag="PLN77_00545"
     CDS             15346..16059
                     /locus_tag="PLN77_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HRM42799.1"
                     /translation="MKKVSLEQELKGNAYPGRGIVIGRSKDGTKAVAGYFIMGRSQNS
                     RNRVFVEEGEGIRTQAFDPSKLEDPSLIIYAPVRVLGNDTIVTNGDQTDTVYEGLEQG
                     KTFEVSLRSREFEPDAPNYTPRISGVMHVENGTYGYQMSILKSNNGDPSQCNRYTFSY
                     DNCVAGEGHFIHTYMHDGNPLPSFEGEPKLVEIPDDIDAFTELLWSSLNEDNKVSLFT
                     RYIDIATGKYETRIVNKNK"
     gene            16091..17269
                     /locus_tag="PLN77_00550"
     CDS             16091..17269
                     /locus_tag="PLN77_00550"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HRM42800.1"
                     /translation="MKELALKYGCNPNQKPSRIYMDDDRDLPIEVLSGRPGYINFLDA
                     FNGWQLVRELKAATGLPAATSFKHVSPAGAAVGLPLDETLRKIYWVDDMGELSPLACA
                     YARARGADRMSSFGDFIALSDVCDKDTASLIKREVSDGVIAPGFTDEALEILKAKKKG
                     NYCVIKIDENYRPAPLEHKQVFGITFEQGRQELPIDDELLSNVVTENKEIPEAAKRDL
                     KIALITLKYTQSNSVCFVKDGQAIGVGAGQQSRVHCTRLAGQKADNWFLRQCPKVLDL
                     QFVDGIRRADRDNAIDVYIGEEYMDVLADGAWEKIFKVKPEVFTREEKRAWLDQMKGV
                     TLGSDAFFPFSDNIERAHKSGVQYIAEPGGSVRDDAVIECCNKYNMAMAFTGIRLFHH
                     "
     gene            17412..17597
                     /locus_tag="PLN77_00555"
     CDS             17412..17597
                     /locus_tag="PLN77_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42801.1"
                     /translation="MKAREKIWKMIKTKILGAKVRYRLFLSKTPVLFRKSVIKYPMGV
                     SFARCKKEDEEYAADGI"
     gene            17578..17820
                     /locus_tag="PLN77_00560"
     CDS             17578..17820
                     /locus_tag="PLN77_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42802.1"
                     /translation="MRQMEFKMERQGLLEEGQEVNVTESALPTSYYYTITPAVAMSRN
                     YQAYERLQSRKGIVKEVKETPRGFYTVVEFDEDEPT"
     gene            17807..18334
                     /locus_tag="PLN77_00565"
     CDS             17807..18334
                     /locus_tag="PLN77_00565"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HRM42803.1"
                     /translation="MSRPDENKNEHPVVHPIPPVYRADSRILILGSFPSVKSRETGFF
                     YGHPQNRFWKVTAAVCGEEVPQTVEEKKAFLLRNHIAVWDVIASCVITGSSDASIRDV
                     VPNPLQDILECADIRQIYCNGATAWKYYRKYQEKQLGREAVRLPSTSPANAAWSVERL
                     LGEWSQCGEVLRNGE"
     gene            18339..18569
                     /locus_tag="PLN77_00570"
     CDS             18339..18569
                     /locus_tag="PLN77_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42804.1"
                     /translation="MKKEYETVWEIFNECANNQMRDVFFDEIETDDPEAYIRQKFPDK
                     NLKYEKTVEADGSLVFDIETSGIRQRFTFTEI"
     gene            18608..18952
                     /locus_tag="PLN77_00575"
     CDS             18608..18952
                     /locus_tag="PLN77_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HRM42805.1"
                     /translation="MSEEKHTHVHVLEDGTVIEHSHDHAHGHHHTNTKAVLNRLSRAI
                     GHMESIKRMVEEGRDCTEVLIQLSAVKAAINNTGKIILEDHIEHCIVDAVEHGDIKAI
                     QELNKAIDRFVK"
     gene            18983..19807
                     /locus_tag="PLN77_00580"
     CDS             18983..19807
                     /locus_tag="PLN77_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRM42806.1"
                     /translation="MAKECSNSSCSKESCEGCPSKAGQGKPDFHIDLNADSSIKHVIG
                     VVSGKGGVGKSMVTASLANQKAKEGYKVGILDADITGPSIPKMYGLKGPANVDDKGVY
                     PAVAANGVKVMSINLLLPGEEEPVIWRGPVIAGTVKQFWTDVIWGDLDYLFVDMPPGT
                     GDVPLTVFQSLPVDGVVIVSSPQDLVRMIVMKAYKMAQMMSVPVLGIVENYSYLVCPD
                     CGKKLSIFGESHIDEIAAEIGVPVLGKMPIHPEFAELVDEGRFAEVDNTDIAKINI"
     gene            20143..20370
                     /locus_tag="PLN77_00585"
     CDS             20143..20370
                     /locus_tag="PLN77_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM42807.1"
                     /translation="MSHCVVRLNGTEEVEEFVKAASKCDFDIDIFYNRVIIDAKSILG
                     ILSMDLNRDLTVHCYGDSPAFNKTLQKFAVA"
     gene            20700..23192
                     /locus_tag="PLN77_00590"
     CDS             20700..23192
                     /locus_tag="PLN77_00590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRM42808.1"
                     /translation="MEEKKKEIRISVRNLVEFILRSGDLDNSSGGFGERDAMQAGTRV
                     HQQIQKKRGADYRAEVPLVHEKEYEHFILRVEGRADGMYEDGTTTVIEEIKGTYRDLE
                     TMEEPVPVHLAQAKCYAYIYGLQNRKEEMGVLMTYTLLSSEEEGLLRPLTKEEVKPEH
                     SNWRIRHFLQTYKLPELEQWFDELLDAWFIWAEFQYQWQKARDASMQHLEFPFPYRKG
                     QRELVSGVYRTILRKKELFVQAPTGIGKTMSTVFPAIRAIGEGCGDRLFYLTAKTITR
                     TVAEEAVSILKEKGLQFKAITLTAKEKMCILDEPECDPIHCPRAKGHFDRINAAVYEM
                     LTECDSYTREEVLRQAEKWNVCPHELQFDVASWVDGVICDYNYAFDPTSKLKRFFAEG
                     TKGDYIFLIDEAHNLVERGRDMFSATLVKEEILKVRQLLRPYAPGKKLEKALNRCNHQ
                     MLVYKRECDSCTELESVSTFLMMVNQLLEELAGWLEAHKTSEIRKQVLEFYFNLRNFS
                     EIYNLVDENYLIYTSYLDNGDFALRLFCVNPAENLQQCINQGRSAVFFSATLLPVQYY
                     KKMFSTNTDDYAIYVESPFDPTKRCLAIGSEVSTKYQRRNRAEFEKIAAYLNEMIQSR
                     KGNYMAFFPSYRLMQDVYAVYEELYADENVTCLIQESAMREQEREEFLEAFAKDNEKT
                     LVGFCIMGGIFSEGIDLDGEKLIGAAVVGAGIPQVGPERELLKQYFDRSGENGFDYAY
                     RYPGMNKVLQAAGRVIRTTEDTGVILLLDERFRNREYRELFPREWEDCRIVQRSSLPE
                     VIHSFWERVDCESGSKAGQQEMKSRYSAERYV"
     gene            complement(23183..24076)
                     /locus_tag="PLN77_00595"
     CDS             complement(23183..24076)
                     /locus_tag="PLN77_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM42809.1"
                     /translation="MQIRMDKENRELKAHGSYEFPVNISYEQLSRYERGSFSWHWHPE
                     IELTFVLSGQIGYQVNGKSYVLKEGDGIFCNTNALHSGHMIDGMDCVYISTTFAPRFL
                     YGFSNSVIQTRYVEPVLTDMQLASIVFSPEIDWQNQVLACMHRIYDLSLSQPSAFEME
                     IQELLLSIWIEIYRHASFSGESQNTAAARDVERLRILLDYIHRHYMEHLTLEDLAAQI
                     HICRSECCRFFKKCMNESLFDYLLDYRIEKSLPLLLERQASITSVAEQTGFSSPAYFS
                     KIFRQQMGCSPSEYRNRADHT"
     gene            24358..25527
                     /locus_tag="PLN77_00600"
     CDS             24358..25527
                     /locus_tag="PLN77_00600"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRM42810.1"
                     /translation="MKQQVLDCFAKLFGGEGDIRTYFAPGRVNLIGEHTDYNGGHVFP
                     CALTIGTYMAARKRDDRKLRFYSMNFEKLGVVESSLDEFQMGKEGNWTAYPKGMMWAF
                     CQKGFPVEQGFDIVLYGNIPNGSGLSSSASVETVTGVMLRDMFGYDTISMIDIALYGQ
                     FSENNYNGVNCGIMDQFAIAMGKKDCAIFLDTSDLKYEYAPVKLEGARIVISCSNKKR
                     GLGDSKYNERRSECETALAEIQKVKDIKSLGELTEEEFEAVKDAIKDPVRQKRAKHAV
                     YENQRTVQAVAALKANDIAKFGELMNASHVSLRDDYEVTGIELDTLVEEAWNCPGVIG
                     SRMTGAGFGGCTVSIVKTEDIDNFIAKVGAAYKEKIGYAADFYVVDIGDGAGVLK"
     gene            25568..27061
                     /gene="galT"
                     /locus_tag="PLN77_00605"
     CDS             25568..27061
                     /gene="galT"
                     /locus_tag="PLN77_00605"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRM42811.1"
                     /translation="MINESIAKLVKYGENAGLVSGLDKIYATNRILEVMQISDYEEPE
                     QIPETPDLEETLNELLDDAAKRGLLEHNSVVYRDLFDTKLMDCLMPRPGEVVKKFEEL
                     YAKSPQEATDYFYKLSQDSNYIRRYRIAKDIRWSVPSAYGDIDISINLSKPEKDPKAI
                     AAAKLAKQSGYPKCLLCKENVGYAGRVNHPARQNHRIIPLTINQTEWGFQYSPYVYYN
                     EHCIVFNFQHNPMKIERATFVKLFDFIKLFPHYFIGSNADLPIVGGSILSHDHYQGGH
                     YTFAMAKAPIERHFTIKGYEDVETGIVKWPLSVIRIRHKDEKRLIDLAEHILNCWRGY
                     TDEDAFVFAETDGEPHNTITPIARKVGDTFELDLALRNNITTDECPLGVYHPHAQYHH
                     IKKENIGLIEVMGLAILPARLKDELEELSKCLVEGKDVRAVAELEKHADWVDEFLPRH
                     PELNSENVMDILKEEVGKVFVGVLEDAGVYKCTEEGRSAFAKFMETL"
     gene            27135..28151
                     /gene="galE"
                     /locus_tag="PLN77_00610"
     CDS             27135..28151
                     /gene="galE"
                     /locus_tag="PLN77_00610"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRM42812.1"
                     /translation="MKILVTGGAGYIGSHTCVELLNAGYDVVVMDNLYNASEKAIERV
                     EQITGKKVTFYKTDMLDREGVKKIFDNEKIDAVIHFAGLKAVGESVHKPIEYYHNNMT
                     GTLILCDEMRNHGVKNIIFSSSATVYGNPAQIPITEECPKGTPTNPYGWTKSMLEQVL
                     TDIHTADPEWNVILLRYFNPIGAHKSGLIGEDPKGIPNNLLPYVAQVAIGKLECIGVF
                     GDDYDTPDGTGVRDYIHVVDLARGHVKAIQKLADNEGVSIYNLGTGKGYSVLDVIHAF
                     EKACGHPLKYEIKPRRDGDIATCYSKCDKAKEELGWEAEYGIEEMCADSWNWQQKNPN
                     GYND"
     gene            28339..30297
                     /gene="ftsH"
                     /locus_tag="PLN77_00615"
     CDS             28339..30297
                     /gene="ftsH"
                     /locus_tag="PLN77_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRM42813.1"
                     /translation="MDDRKNQNREPENGSGGGPKNRQTILIILICLLVSLMVMGLLNN
                     ALGGITSEDLDYSKFLDMVNNDEVQEVVLKSGTLTVTPKDQKSEGITYQVTMMGDEQE
                     LVDLLQEKGITYHKDPPDSTGTIIYTLLTVILPVVMLVAVMVWVMRSMNKGGGVMGVG
                     KSRAKAYVQKETGVTFRDVAGQDEAKESLQEVVDFLHNPGKYTTIGAKLPKGALLVGP
                     PGTGKTLLAKAVAGEAHVPFFSMSASEFVEMFVGVGASRVRDLFEEAKKNAPCIIFID
                     EIDAIGKSRDNSYNSNDEREQTLNQLLAEMDGFDSSKGILILAATNRPEVLDPALLRP
                     GRFDRRIIVERPDLKGRVEILKVHAKNVSLDETVDLDGIALATSGAVGSDLANMINEA
                     AILAVKNGRKAVSQKDLLEAVEVVLVGKEKKDRILSQEERKIVSYHEVGHALVTALQK
                     DSEPVQKITIVPRTMGALGYVMQVPEEEKYLNTKSELEAMIVECLGGRAAEEIVFGNV
                     TTGASNDIEKATKIVRAMITQYGMSEKFGLIGLATQQNQYLDGRMVLNCGDATATEID
                     HEVMKILKESYEQAKELLAANRDAMDEIAAYLIQKETITGKEFMEIFHQVIAKRNGNA
                     EEAVENPENTETENTALPEADFTVNPEV"
     gene            30390..32249
                     /gene="uvrC"
                     /locus_tag="PLN77_00620"
     CDS             30390..32249
                     /gene="uvrC"
                     /locus_tag="PLN77_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRM42814.1"
                     /translation="MAFDIQEELKKLPGKPGVYLMHDADDTIIYVGKAISLKNRVRQY
                     FQTSRNKGPKIERMVTHIARFEYIVTDSELEALVLECNLIKEHRPKYNTMLKDDKAYP
                     YIKVTVNEDFPRILFSHQMKKDKAKYFGPYTSAGAVKDTIELLRKLYDIRSCNKSLPK
                     EIGKDRPCLYYHIHQCKAPCQGYISKEEYGEQIKKAISFLNGNYNDIIKELTGKMTEA
                     AEEMRFEQAAEYRDLIDSVRRIGERQKITNSDGEDKDVIALAMDKEDAVAQVFFIRNG
                     KLIGREHYYLRIGEEEQKSEVLLTFMKQFYSGTPFIPREIMISEEIAEQELMEEYLSS
                     KRGQKVRIRVPKKGSKEKMVELAERNAQIVLDQDRERIKREEGRTVGAVKEIARWLNL
                     PSIDRIEAYDISNISGFQSVGSMIVYEKGKPKRADYRKFKIKSVEGPNDYASMKEVLS
                     RRFLRGIQEDSGFERLPDLIMMDGGRGQVNIALEVLEEMDLAIPVCGMVKDDKHRTRG
                     LYFRNVEIPIDRNSEGFKLITRVQDEAHRFAIEYHRLLRSKGQVHSILDDIPGIGPTR
                     RKELMRHFQGIDDIKAATVEELSGLPGMNKKAAEQVYTFFHAPAQEENEDSSK"
     gene            32344..33279
                     /gene="hprK"
                     /locus_tag="PLN77_00625"
     CDS             32344..33279
                     /gene="hprK"
                     /locus_tag="PLN77_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRM42815.1"
                     /translation="MKEIEMTKMVEEMDLRNLTPEVDMTDLVIATPDINRPALQLTGY
                     YEHFANERVQIIGYVEFTYLQHLEESERVRAFEEFVSRKIPCVIFTTKMIPGPEILEL
                     CNRYQVPAFAVDKSTSVFMAEIIRWLNVQLAPMISIHGVLVDVFGEGVLIMGESGIGK
                     SEAALELIKRGHRLVSDDVVEISRVSDITLVGTAPDITRHFIELRGIGIIDVKTLFGV
                     ESVKDTQSIDLVIKLEEWDKEKEYDRLGLEEEYIEFLGNKVVCHSLPIRPGRNLAIIV
                     ETAAVNHRQKKMGYNAAQELYRRVQANLVKKREEK"
     gene            33284..34228
                     /locus_tag="PLN77_00630"
     CDS             33284..34228
                     /locus_tag="PLN77_00630"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HRM42816.1"
                     /translation="MANYVFGIDVGGTSVKCGLFQTDGTLLEKWEIPTRTENGGSEIL
                     PDIAKSVKAKMGEKGIAADDVAGVGIDVPGPVNDKGELSIAVNLNWGYKNIVKELSDE
                     LGGMAVKAANDANAAALGEMWAGGGKGSKNLVMVTLGTGVGGGIIVDGKIVAGAHGAG
                     GEVGHACVDPEEEAVCNCGNHGCLEQMTSATGIVRLAKKYLASHDTPSSLRERGESIS
                     AKAVFDALKEGDAAAEAIVQEFSAYLGRALAVFACVVDPEVIVVGGGVSKAGQILIDG
                     VAKYYREAAFIACKDTPIVLASLGNDAGIYGAAKMLID"
     gene            complement(34320..36899)
                     /locus_tag="PLN77_00635"
     CDS             complement(34320..36899)
                     /locus_tag="PLN77_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRM42817.1"
                     /translation="MHFGKSHLEDKREDLRSHYGKIEKTAGVTALRIAFCLLLVVLVC
                     GTGLVIGAVRGVIDSAPDISEANIMPLGNASFIYDADGNQVQKLTGAQGNRVSISIEE
                     IPLDMQHAIVAVEDARFYEHNGIDPSGIIRAFVVGVSHGFHFTEGASTITQQLLKNNV
                     FTDWMEETRMQSFKRKIQEQYLALKLEKTLTAEGENAKDVILENYLNTINLGSGCYGV
                     QTAAQTYFGKDAKDLTLSECAVLAAIPKAPTAYNPKNHPEKNQQRRDKILNNMKEQGY
                     ITEEEYTIAMNDNVYDRIAMHTQSQAESAPYSYFIDEVITNLINDLMVQKGYTEVQAK
                     NVVYSGGLKIYTTQDSYMQSILDTEFQNPENFPANTQIGLDWALTVEQADGEVQNYSK
                     EMLQLYFRNSDPNFDLLFDSQEEAQSYIDQYKAAIMQEGDTIVAERSSFTPQPQACMT
                     VMDQRTGYVKAIVGGRGEKTASLTFNRATDNYSQPGSTFKILSAYGPALDLGKITLAT
                     VIKDEPFNYSDGTPLQNSDLTYHGDVTVRQAIINSINIPAVKVLTELTPKVGFDYLKK
                     LGFSKLSEEYDVIQPLALGGITYGVSDLELTAAYAAIADGGQYRKPVFYTKVTDSKGN
                     VLIDNTENKATQVFKASTASLLTNAMEDVLEKGTGVAARLDNMHAAGKTGTTNEAKDL
                     VFAGFTPYYTAAIWATYDTHAEFPESDREFHKRLWAKVMNEIHEGLADMDFETSATVQ
                     EATICTKTGLLARSSCPSITEYFAVSDLPTERCKGHYTAPTSTPTPTRTPSAASTPQA
                     TPTPTEAPQETPAPPETPADPPSNTPETPDTPDTPDAPSEPDTPAETSEVQPQSEESG
                     SSE"
     gene            37105..37800
                     /locus_tag="PLN77_00640"
     CDS             37105..37800
                     /locus_tag="PLN77_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRM42818.1"
                     /translation="MIDQFRTVYRGGTGEIVEKKSRFIATVRLVESEEEALSCLEALR
                     KKYWDARHNCFVYIIGENQETVRCSDDGEPSGTAGRPMLDVVQGAGLRNVLVVVTRYF
                     GGTLLGTGGLVRAYSQAVQEGLANSVLIDEICGVRLLIETDYNGIGKIQYLLGQRGIP
                     ILESEYTDQVKIRVLVPKTEVDRLCAEITEGTNGKAKLEKEEELYFAQNDGELIFGDE
                     LTQYKNLEAEELG"
     gene            37814..38798
                     /locus_tag="PLN77_00645"
                     /pseudo
     CDS             37814..38798
                     /locus_tag="PLN77_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559607.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
     gene            complement(38871..39554)
                     /locus_tag="PLN77_00650"
     CDS             complement(38871..39554)
                     /locus_tag="PLN77_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM42819.1"
                     /translation="MSSFLESCFQEHYPFYSKLTADEQQTLVLSSVLTTYQRGDFLYS
                     CDDDCLGVLLVLSGKLRIFIQSGEQREITLFTTGPGEVCTLSASCAMQEISFDILIEA
                     EEETKVLITPASTFRRLLNQNIYVENYIYRETTEHFSEVMWAMGQLLFTRLDQRLAGY
                     LEDERIRTGSPVLSTTHEQIARNLGSAREVVSRTLKSFEKDGIVALSRGTVTILDVKK
                     LRGICRMRR"
BASE COUNT        11045 a         9324 c        11116 g         8243 t
ORIGIN      
        1 gaatcacttt cttacgcagc gaaacagcaa gtgtttcact gctgagtgaa cagtaacgaa
       61 aaatccacag cagtaaaatg acaaaaatca gattctaaat aattgcagca aaaatattcg
      121 cgcatcaaat atcttaaaaa ctccaaacag aaccgaaaat caaatagaga atagatagag
      181 gaaaaatggt atgacaagag aaaagtatga gtcattgcct ttgagcacgc tgaaagcgct
      241 ggcaaaatcc agaaacatga aggggatttc caccctgcgg aaagaagcgc tgattgagcg
      301 gatgctggaa gaagatcaga aagaaaacca gaaaaacatg gaagagccgg cggttctaaa
      361 agaggaaaaa aagacggaaa ctgtacagga tcagaatcag gaaccgcagg aaaagcggga
      421 ggacggtatg gagcgccaga atcgggaaac ctcggaaaac ggcaccgcgc gcccgtacca
      481 gagaccgcgg tatgtccgcc gggaagagcg cagtgaaaac ggagagcgcc aggagtaccg
      541 tccaagagcg ccgcgtttca atgagcgtag atttcaggaa acacggaatt ataatcagaa
      601 taaccgtgag gaagagcaaa gacaggagcc gcgtcaggaa gcagggcagg aaatgagaca
      661 ggagccgctg cgtcaggaag caagaccaga gacgagacag gaaccgcgtc aggaaaccag
      721 cacgctcgac agcggccagg aggcctgcgg catcctcgag gtcatgccgg acggatttgg
      781 atttatccgc tccgacaact atatgccggg cgacagcgat gtctacgttt ccccgtccca
      841 aatccgccgt ttcggactga aaacgggaga tatcatccgc ggaaacacga gagtaaagtc
      901 cgcaaccgaa aaattcagcg cccttttata cttaaagagc gtcaacaatc tgccgctcga
      961 ccagatcatg cgccgcggca atttcgaaga tatgacaccg gtcttcccgg atgagcggct
     1021 gcgccttgag cgcccgggcg gaagcatggc gatgcggatc gtggatcttg taagtccgat
     1081 cggaaaaggc cagcgaggca tgatcgtctc cccgccgaaa gccggaaaaa cgacgctttt
     1141 gaaggatgcg gcaaaatcca tcctgcggaa caacccggag atgtatctga tcatccttct
     1201 gatcgacgag cgtccggagg aggtcaccga cattaaagaa gcgattcagg gatcgaatgt
     1261 ggaagtcatc tattccacct tcgatgagca gccggagcac cacaaacgcg tgtccgagat
     1321 ggtcattgag cgcgcgaaac gtcttgtgga gagtaaaaaa gacgttacga tcttcatcga
     1381 cagcatcaca cgtctcgcca gagcttacaa cctgaccgtt ccgccgagcg gccgtaccct
     1441 ttccggcggt ctcgatccgg cagcactcta catgccgaaa cggttcttcg gcgcggccag
     1501 aaacatgcgc gagggcggaa gcctgacgat tcttgcaacg gcgcttgtgg acaccggaag
     1561 caagatggac gatgtcgttt acgaggaatt caaaggcacc ggaaacatgg aactcgtgct
     1621 cgaccggaaa ctgcaggaac gccgcgtctt cccggccatc gacatagcca aatcgggaac
     1681 aagaagagaa gatctgcttc tgacaccgga tgagcaggag gccgtttaca atatgcggaa
     1741 ggcattaaac ggcatgaaat ccgaggaagc cgtggaaaat attctgaaca tgtttgcccg
     1801 caccagaaac aacggcgaac tgattcaaat tttaaagaaa caaaaaatag tataaggctt
     1861 gcaattttca aaattgtgtg atacaatgag aaagctgtgc aaggttgatg agattttgtc
     1921 agcctgaaat acgaaatcat cagagtaaat ctgagaagat aacaatatta agaagaggtg
     1981 aataaaatga gagaaggaat ccatccagaa taccatcagg caactgttac ctgcaactgc
     2041 ggaaatactt tcgtaacagg ttctaccaag aaagagatcc acgttgagat ctgttctaaa
     2101 tgccatccgt tctacactgg ccagcagaaa gctgctgcag ctcgtggacg tatcgagaag
     2161 ttcaacaaga aatacggcat gaaataagaa ttgaatcaag caagatgaaa aacgggctga
     2221 ggttggaaga tctcagcccg ttcttgcgtt acggatgcag aaaatcccct ttgaggaggt
     2281 aaacgtaaat tatgaaatca tccaatattg gcggccaggc cgtgatggaa ggcgtcatga
     2341 tgatgaacaa ggaccgctat tccgtcgcag tacgccggcc ggacggggag attgaggtta
     2401 aagtagagca gcacaagccg ctgacaaagc acaaggggct tttaaaagtt ccgattctgc
     2461 gcggtgtctt cagctttgcc gattcgatga tcgtcggcat gagttccctt atgtactccg
     2521 cttcgttcta tgaggacgag gaggaggaaa agaaaaagcc ggcgacgaaa gaggaagagg
     2581 agcagcagaa agcgaaaaaa gaaaagacag acaggctgat gatgtacgga accgtcacat
     2641 tttccgttgt catcgccgtc gttctctttc tgatggtgcc gtacttcctg tccgaactgc
     2701 tgcacaaagc gggagccgga acgacactga ccgcggtggc agaggccttt gcgcgtgtgg
     2761 cactgttcct cggctatctg gcagccattt cccgtatgga agacattcag cgcgtgttca
     2821 tgtaccacgg cgccgagcat aaatgcatca actgcgtcga gcacggtctg gaactgaacg
     2881 tggaaaacgt cttaaagagt tcccgggagc acaagcgctg cggaacgagc tttcttatgt
     2941 acgtcatcgt catcagcatc atctttttca tgttcatccg ggtcgattcc ccggtgctga
     3001 aaattgtgat ccgcctgctt ctcattccgg tgatcgcggg cgtttcctat gagttcatcc
     3061 gtctggcggg aaccagcgag aataagatcg taggtctttt gagcaaaccn nnnnnnnnnt
     3121 gaaattatcc gcctggcggg aaaaagtgac aataaactgg tcaacctgat cagtaaaccg
     3181 ggcctgatgc tgcagcacct gacgacgcgc gagccggatg ccgagatggc agaggttgcg
     3241 atcgccgctg tggaggctgt gtttgactgg agagcgtacc tgaacgaaaa ctttggttat
     3301 acgtacccgg ccaaaaccga aaagaaagaa catgaggagc cgtgaccgat gagtgtgcac
     3361 gatagaaaaa actgcactgg aatccgctat tctgtactta caaaagcagg gggtggagga
     3421 agcccggcag gacgcatggc ttttactgga gcatgtctgc gggatcagcc acagcaccta
     3481 ctttgtacac agtgaagatg aaatgccaaa agaacagcag gagcagtacg aagcgctggt
     3541 cagaaagcgc ggcgaacaca ttccgctgca gcagctgacc ggagaggcgt ggttctacgg
     3601 aatccctttt tttgtgaatg agcatgtact gattccgcgg caggacaccg agattcttgt
     3661 ggaggaagcg ctccgcggcc ttccaaaaga tgcacggatt ctcgatctct gcaccggttc
     3721 gggatgcatc ctcatatcgc ttcttgtgac gagagaggac tgcacgggaa tcggcgcgga
     3781 tctctccgag aaagccttac aggttgcgga aaagaacggc gcacaggcgg gcggacgggc
     3841 atcgttcgta aagagcaact tatttgaaaa catcgaggga accttcgatg tgatcgcaag
     3901 caatccgccg tatatccgca cggatgtgat tgaaacgctg atgccggagg tgcgcgacca
     3961 tgagccgcgg atggcactcg acggcagtgc ggacggtctt gtcttttacc ggaagattac
     4021 agagcaggca ggaagctatt taaaagaggg cggaaagctc tgttttgaaa ttggatacga
     4081 tcagggagat gaagtgcggg agctgatgga aacccacggc tttgccgatg tacaggttct
     4141 gaaagatctg gcaggcctgg accgcgtggt gaccggtatc tggcacaaaa aaccctgaca
     4201 gaaatgaatt ttaattttga caccataatg acatcataaa cagacgaaaa cagcaaaaag
     4261 gagcagtcat tttatgtttg ataaacttga tgatatcctc atgcgtctcg aggaggtact
     4321 gaatcagtta agcgagccgg acgtcgctgc cgatgcggcg aagttccaga agctgatgaa
     4381 ggagcaggca gaactccagc cgatcgcaga cgcatataag gactataaaa cacagaaaca
     4441 gaccatcgaa gagagcctga tgcttctcga ggaggaatcc gacgaagaga tgcgcgaaat
     4501 gctgaaagaa gagctctccg atgcaaaaaa acgcgtagag gagctggagc aggagctcaa
     4561 agtcctgctt ctgccgaaag acccgaacga tgacaagaac gttatcgtag agttccgcgc
     4621 aggtgccggc ggagacgagg cggcactctt taccgcagaa atctgccgta tgtacatcaa
     4681 atacgcagag agccgcggct ggaaaacaga gctgaccagc gccgatgaaa acggaatcgg
     4741 cggctttaaa gacgcaacac ttctgatcaa cggagcaggc gcgtactccc ggatgaagta
     4801 cgagagcggc acgcaccgcg tacagcgtgt gccggaaacc gagtccggcg gaagaatcca
     4861 tacctcaacc atgaccgtag cgatcatgcc ggaggtggac gacgtcgaag tacagatcga
     4921 cccgaaagat tacaaattcg atgtattccg tgcttccgga aacggcggcc agtgtgtcaa
     4981 tacgaccgac tcagcagtcc gtctgaccca cttcccgagc ggcatcgtca tttcctgcca
     5041 ggatgagaaa tcacagttaa agaataagga caaggcactg aagattctgc gtgcccgcct
     5101 gtatgactta gagcagcaga aacagcacga tgagatggct gagcttcgaa agagccaggt
     5161 cggaacggga gaccgctctg agaagatccg tacgtacaac ttcccgcagg gacgtgtgac
     5221 ggatcacaga atcaaactga ccttatacaa actggacgcc atcatgaacg gcgatctgga
     5281 tgaaatcatc gacagtctga ttgcagccga tcaggccgca aagctgagca gcatgcagga
     5341 agaagcataa taataaacgc tgcgaatcac gattggtcta caggatcgat tgttgataga
     5401 ataaacaaga gaaaaaaggg gaaaagagaa agggaaaacg ctatgaagaa aacagcactt
     5461 gtgatcatgg cagccggaat cggcagcaga tttggaaagg gaatcaagca gcttgagcca
     5521 gtaggaccaa acggggagat catcatggat tattccatcc acgatgcact ggaagccggc
     5581 ttcaacaaag tagtcttcat catccgcaag gatctggaga aagatttcaa ggaagtcatc
     5641 ggcaaccgca tcagcaaaat cacagaggtg gaatatgcat tccaggagct cgatgatctg
     5701 ccggcaggct ttgcaaaacc ggaaggccgt accaaaccgt ggggaaccgg ccaggccgtt
     5761 cttgcatgca aaggtcttgt aaaagagccg tttgctgtta tcaatgctga tgactattac
     5821 ggaaaagatc cgttcgtaaa actgcacgac tacctcgtac aggagcatga tgtggatgaa
     5881 gttatgccga tctccatggc aggattcgtg cttggaaaca ccttaagtga caacggcggt
     5941 gttacaagag gtgtctgcgt cgttgatgaa aacggaaacc tgaccggtgt caacgagacg
     6001 aaaaacatcg taaagaccgc agagggcgca ggcgttgaaa aagaagacgg aacggttgat
     6061 ccgatcgaca aaaactccct tgtttccatg aacatgtggg gactgcagcc ggagttcatt
     6121 gatcttctgg agaaaggctt tgttgaattc ctttctgcac agaaagaggg agatgtaaaa
     6181 ggcgagtacc tccttccgat ctacatcgac gagctgctgc acgcaggaaa agcagaagta
     6241 aaagttcttc cgaccgacgc agagtggttc ggcgtaacgt atcaggaaga caaagcttcc
     6301 gtaacagaag cattccgcaa actgattgcg gacggcgtat acagcgagaa gctgtacgac
     6361 taattacgat attttgttat ttgagtgaat aaccagaaaa agcgtgtttg aatgattgca
     6421 aaaaatttca gacacgcttt ctgcccgaaa ggggaaagga gcataccatg ggtaaatatt
     6481 ttggaacaga tggatttcgc ggagaagcaa acattgatct gacggttgag catgcatata
     6541 aagtgggaag attcctcgga tggtatttcg gaaaagacca caaagcacag atcgtcattg
     6601 gaaaagacac cagacgctcc agctacatgt tcgaatattc gctggtagca ggactgaccg
     6661 catcgggagc agacgcttat ctgctgcacg taacgaccac cccgagcgtt tcctatgttg
     6721 tccgtaccga aaactttgac tgcggaatta tgatctccgc cagccacaac ccgttctatg
     6781 acaacggaat caaagtcatc aacggcctgg gacagaaact ggaagccgac atcgaagcgc
     6841 agatcgaagc ctacatcgac gacggaacac cgaagatccc gttcgcaacg agagaagaca
     6901 tcggacgcac cgtggatttt gcagccggaa ggaaccgcta cattggacac ctgatctcga
     6961 ttccgacaaa atcctttaaa aacatgagag tcggactcga ctgctccaac ggaagtgcct
     7021 cttccgtagc caagagcgtg tttgatgcca tcggcgccaa gacatatgtg atccacaacg
     7081 agccggacgg aacaaacatc aactgcggct gtggttccac ccacatcgaa gagcttcaga
     7141 aatatgtaag agagaaacac ctcgatgtcg gatttgccta cgacggagac gcagaccgct
     7201 gcatcgctgt cgatgagctg ggaaatgtgg tagacggaga cctgatcatg tacgtctgcg
     7261 gtaaatactt aaaagaaaaa ggccagttaa acggagatac gatcgtgacc acgatcatgt
     7321 caaacctcgg tctttacaaa gcgtgtgacc gcgccggtat ccggtatgag aagacggcag
     7381 tcggcgacaa atacgtgtgc gagaacatga tggcaaacgg ctattctctc ggcggagagc
     7441 agtccggcca catcattttc agcaagcacg cgacgaccgg tgacggaatc ctgacctccc
     7501 tgatggtgat ggaagtgctg atagataaga aactcccgct cagcaccctg acagccgagg
     7561 tagtgattta cccgcagctc ttaaagaatg tccgcgtggc aaacaaagca gctgcaaagg
     7621 ccaacctggc agtacagaaa gcagtagaag acgtagcaga ggccctgggc gatgacggac
     7681 gcatcctcgt aagagagagc ggcaccgagc cggtgatccg tgtcatggtg gaagcaccgg
     7741 acgacgcaac gtgtgcaaaa tacgtagacc aggttgtttc tgtgatgcat aaagaaggac
     7801 ttgtgacaga atgaaaaaaa gactggtaat cttttttctg gcggcggcaa tggtgcttgg
     7861 cggctgtgaa aataaaaatt caaagcttta taaaaagggc atcgaggcgc tcgaacagaa
     7921 ggactacgta acgtcgattg cgatgctgga gggagccgta aaggcaggcg accgactggc
     7981 ggagagctac cggtcgctcg gcatcgctta tctgaaaagc caggagtatg acaaggcaaa
     8041 agaggctttc aagagcagtc tttcgagcat gaagcacaaa gacgcggagt tttcgcgcga
     8101 tgtcatgtat tacgaggcgg aaacctgcgt acaggccgga gatctggatg gagccattga
     8161 gatctgcacc aatattcagg aagaaaaggc cgatgccgat gcgctgttcc ttaggggacg
     8221 cgcgtacttc ctgcagaaga attacgagca ggcgaaggtg gattttgatg cggcagtgga
     8281 gacgaaggag agctatcagc tctgccttga catttacgag ctgtatcagg agagcagcat
     8341 gaaggcggac ggagaccgtt acctcgaagc ggcagtgaag attgagccga aaacgaccga
     8401 ggattattat aacatcggct gcgcatacta ctatctggag gagcaggacg aggcggtcaa
     8461 agcgttctcg aaggcaatga aggacggcag cagcgcggcg atggaaatgc tcggcgaggt
     8521 ttacctttcc aatggtcaga acgacgaggc gaaagcactg ttttcttcgt atctttcgac
     8581 agatggcttt gcggcggcag caaacaacgg gctggcgctc tgcgcacttg cggaaaacga
     8641 cacggacagt gcactctcgt atatcgagga aggactcaaa acggcagagg attccgaccg
     8701 ggaaaatctg ctgttcaacc agattgtatc ctatgaaaaa cgggcggatt ttgataccgc
     8761 gaagtcgctg atggcggatt ttctggcagc atacccggaa aacaccgcgg cgcagcggga
     8821 aaatacgttt ctccagaacc gataacagac ggaggaagtt ggattgtttg atttaagaga
     8881 agaacaggag cgcgtgattc tggtcggcgt acaggaaaac ggcggcgcaa acgcagaaga
     8941 atcgctcgac gagctggcgg aactggccag caccgccggt gcgaaggtgg agggccgcct
     9001 ggtgcaggtg cgggaggcca ttcatccggg aacgtacatc ggaaaaggaa aattagagga
     9061 attgaaaatt ctggtgcgcg cctgcgatgc gacgggcatc atctgcgaac tgctgtatga
     9121 cctggatgct accggcgtga tctgcgacga tgagctttcc tcgatccagt taaaagggct
     9181 ggaggaagca ctggaatgca agatcatgga ccgtacgctt ttgatcctgg atatctttgc
     9241 ggcgcgcgcc gtgagcagcg agggtaaaat ccaggttgag ctggcccagt taaagtaccg
     9301 tgcgtcccga ctttccggcc ttggcacatc gctttcccgt ctcggcggcg gaatcggtac
     9361 gagaggaccg ggagagaaaa agctggaaac cgaccggcgc ctgatccgca cgcgtattac
     9421 gcagttaaaa cgtgagcttg aggaagtacg ccagcacaga gagctccttc gtgaaggcag
     9481 aaaacgttcc aaaatcccgg tagcggcgct cgtaggctat acgtcagcgg gaaagagcag
     9541 tcttttaaat gcactgacag gatcggaaat tctggcagat gcaatgctgt tttccacgct
     9601 ggatacgacg acgcggtcac tgacccttcc ggatggacag gaaatcctgc tgaccgatac
     9661 ggttggattt atcaataagc tgccacatca tctgatcgat gcattccgca gtacgctgga
     9721 ggaagcacgg tatgccgact atattctcca cgttgtggat acgtcaaatc cgcagatgga
     9781 gctgcagatg caggtcgtgt acgatacgct gcgggaactg aaagttgagg acaaaaagat
     9841 cgtcaccctg ctgaacaagc aggataagct tctcgccccg atggttgtaa aagatttccg
     9901 ggcagacgcg agccttgcgg tctctgccaa aacaggacag ggactcgaag acctcaaaaa
     9961 tttactctcc tcgtggatga tggagggaaa aatctatatc gagcgcctct atccgtacga
    10021 taaggcggga acgatttcac tgatccgcgg atacggaatt ctgcttgaag aagaatatcn
    10081 nnnnnnnnng ccggaaggca tccgcgtaaa agcctatgta ccgaaggata tctatccgag
    10141 ggtatagagg tatggcaagc aggcttaaaa acggcagtac atccctttaa gcgccgtaca
    10201 gcgccgaaaa atggaaggga cagaacacaa gtaaaagtac catatctagt gatgttagaa
    10261 aatttcacac tagatatggt actttttact attgacggga gaaacatgct ctgctatcat
    10321 atggttcaga cactgcaaag agtggaacct taaaaaggcc ttgcagaaac ggttgaatta
    10381 acaggaggga ttccatgttt caggtagaaa agagagacgg aacgattgca gagttccaga
    10441 tgaaaaagat taccgatgcg attggaaaag cattcggcgc aaaagatatg cagttcagcg
    10501 atgacatgct tcagatgctg gcactgcgcg taacagcgga ctttcagtcc aagatcaaag
    10561 acggaaaaat ttcagtggag gcaattcagg acagcgtgga gaacgtgctg atccagtgcg
    10621 gctacgcaga agtggcaaaa gcttacattc tgtaccgcaa gcagagggaa aaaatccgca
    10681 acatgaaatc gacgatcgta gactacaaag agatcgtgga cagctacgta aaagtagagg
    10741 actggcgtgt caaagagaac tcgaccgtaa cgtattctgt cggcggtctg atcttaagca
    10801 attccggcgc gatcacagcc aactactggt tatcagagat ttatgatgac gagatcgccg
    10861 aggcacaccg caacgcggat attcacatcc acgacctgtc gatgctgacc ggctactgtg
    10921 caggatggtc gttaaaacag ctcatcaaag aaggacttgg cggcatcaac ggaaagatta
    10981 cttcttctcc ggcaagacat ctttcggttc tgtgcaacca gatggtcaac ttccttggca
    11041 ttatgcagaa cgagtgggca ggtgcgcagg cattttcttc gtttgatacg tatctggcgc
    11101 cgttcgtcaa agtggacaac ttaagctacg gcgaagtgaa gaagtgcatc gaggcgttta
    11161 tctacggcgt caacacaccg agccgctggg gaagccaggc accgttctcc aacatcacac
    11221 tcgactggac cgtaccggat gacctcgcgg agcttccggc gatcgtcggc ggcaaggaga
    11281 tggacttcaa gtacaaagac tgtaaaaaag agatggatat ggtcaacaaa gcgttcatcg
    11341 agacgatgat cgagggagat gccaacggaa gaggcttcca gtacccgatc ccgacctact
    11401 ccatcacatc cgattttgac tggtccgata cggagaataa ccgtctgctg ttcgaaatga
    11461 ctgcaaaata cggtaccccg tacttctcca actacatcaa cagcgatatg aaaccaagcg
    11521 acgtgcgctc catgtgctgc cgtctccgcc tggatctgcg tgagcttcga aagaaaaccg
    11581 gcggattctt cggttccggc gagagcaccg gatcggtcgg cgttgtcacc atcaatatgc
    11641 cgcgtatcgc atatcagtcc aaagatgaga aagagttcta ccgccgtctc gatcatatga
    11701 tggatattgc cgcacgttcg ttaaagacga aacgtgaggt cattacccgc ctcctggacg
    11761 agggactgta tccatatacg aaacggtacc ttggaacgtt tgagaaccac ttctcaacca
    11821 tcggtctgat cggtatgaac gaggccggct taaatgccgc atggctcgga aagggtctgg
    11881 aggatccgaa gacacagcag tttacgaaag aagttctgaa ccatatgaga gagcgtcttt
    11941 ctgattacca ggaggagtac ggcgatctgt acaaccttga ggcaactccg gcggagtcga
    12001 ccgcataccg tctggcaaaa cacgaccgtg caaaatggcc ggatatcaag accgcaggcc
    12061 atgagggaga taccccgtac tacaccaaca gctcccatct gccggtggac tataccgagg
    12121 atattttctc tgcgcttgat attcaggatg atctgcagac gctgtacacc tcgggaactg
    12181 tcttccatgc gttccttggc gaaaaactgc cggactggaa agcagcagct tcccttgtgc
    12241 ggaagattgc agagaactac aaactgccgt actacacgct gtccccgacg tattctgtct
    12301 gtgcgaacga cggctatctc gccggcgagc actttacatg tccgatctgc ggaaaagagg
    12361 cagaggttta cagccgtatc acgggatatt accgtccggt caagaactgg aacgacggaa
    12421 aacgccagga gtacaagaac agaactgtat atgatatcat ccactccaaa tcgccggagc
    12481 agaaaatgaa atcgtacggc gcagcagaga aactggcaga gcaggccgct ggaaaagaag
    12541 agccgaaagc cgctgcagca gcagataaaa tcgaggaaga cggcatgtac ctgttcacga
    12601 caagcacctg cccgaactgc aaaatggcaa aagaaatgct cgccgaggag cagtatgagg
    12661 tgatcgacgc agagcgtcac ccggatcttg ccgcacagta cggcatcatg caggcaccga
    12721 ctcttctcgt tgtggaggac ggccaggcgc agaagattgt gaatgcttcc aatatccaga
    12781 aatatgtgga tgaatatctc tcctaaatct gtgcgcatat agttgcagga aagaacaaac
    12841 agagttataa taggaaaaag tgataccctc gcccgggcat cccgggtggg ggtattatta
    12901 tggcgctgag cacagcagaa atcggcccgg tggaccggtt tcacttacag aagggagaaa
    12961 aattatggga tacagccagg acacaatctg tgtacaggga ggatggcagc cgaaaaacgg
    13021 tgagccgcgt gtacttccga tttaccagag cacaacattc aaatatgcaa ccagcgagga
    13081 aatgggaaaa ctgttcgatc tggaggagga gggatatttc tattcccgtc tgcagaaccc
    13141 gacgaacgac gtcgtagcac agaaaatctg cgatctggag ggcggcgtgg cagccatgct
    13201 cacatcttcc ggccaggcgg caacgatgct tgctgttacc aacatctgtg aggcaggcga
    13261 ccacgttgtc tgcgcggcaa aggtttacgg aggaacctcc aacctgctcg ccgttacctt
    13321 aaaacgattc ggcatcgaga ctaccctcgt ggaccaggat gcgccggcag aggagctgga
    13381 aaaagcattc cagccgaaca ccaaagctgt ctttgccgag acgatttcca acccggcagg
    13441 tgttgtactt gacattgaca agtttgttaa acttgcacat gagcacggcg ttccgatgat
    13501 ctgcgacaac acctttgcaa ccccgatcaa ctgccgtccg ttcgagttcg gtgtagatat
    13561 tgtcacccat tccaccacaa aatacatgga cggccatgca atggcgctcg gcggagcgat
    13621 tgtcgattcc ggaaacttcg actgggatgc gcatgcagac aaattcccgg gtcttaccac
    13681 accggacgag tcgtatcatg gtgttattta cacccagaaa ttcggcaaaa aagcatacat
    13741 cacaaaagca accgcacagc tgatgcgcga tatgggagca tgccagtccc cgcagaatgc
    13801 atttctgacg aatgtcggcc tggagaccct gcacctgcgc gtggagcgtc actgcagaaa
    13861 tgccgagaaa gtggcagagt tcctgaaaaa ccacccgaag gtagcatggg ttgagtacgc
    13921 aggccttgca gacagcaaat accatgcgtt ggcagaaaaa tacatgccga acggaacctg
    13981 cggcgttctc tgcttcggac caaagggcgg aagagacggc gcgatgcgct tcacgaacgg
    14041 cctgaaaatg gtagcaattg taacacacgt tgccgatgca agaagctgcg ttctgcaccc
    14101 ggcaagccat acccatcgcc agctgaacga tcagcagctc atcgaagccg gtgttctgcc
    14161 ggatctgatc cgtctgtcag tcggcatcga ggatgtgaac gatatcattg cagaccttga
    14221 tcaggcgctt gcgaacgtat aaagatacaa aaaacaggat gtgaaaagga gcgtattggt
    14281 aaaatggaag aaatggcaaa gaaacgcgta aaagattctg agacagagca gtcgtatctt
    14341 ctgatgcacc gccatatcaa cggctatggc cgtctgttcg gcggccagct ggtcgcgtgg
    14401 atcgatgagc ttgccggtct ggtgggacgc cgccactgcg gccacgaaat caccaccgcg
    14461 tgcatcgaca atctgaactt caagcatcct gcttttctga acgacaccgt cgtgctggtc
    14521 ggaagagtga cgtatgtcgg acgcacctca atggaggtgc gcgtcgacac gtacgtggaa
    14581 gagcttgcga ccggcattcg gaaaatgatc aaccgtgcct acgtggtgat ggtggccatc
    14641 gacgagaacg gccgcgcact tcccgtcccg ggcctgattc tggaatcccc ggagcaggag
    14701 gccgagtggg agggcggaaa acgccggtat gaactgagga aagagcgtca cagagagggc
    14761 ttctaaaaga ggctctcttt ttttcgaaaa attccattga catttctgtt tcttacgccc
    14821 tataatgcca gataacggtt cgtaagaact aatagaaatt accataaagc aactacaaaa
    14881 gaatcaggag acacaaaaat gaagaaactg aatcgagggt ggattatctg gaagcggcgt
    14941 atgttttaaa actccgcaga ggctacgtcc gaaatgcgat gatctgcaga tatatggggt
    15001 attcacaggc aagtatctcg gccggcatca aaatgctgcg ggaagacggc tgtctggtcc
    15061 gcgacagtga cggttttctg gacattacag aaaaaggcga gacactcgca aagaaaattt
    15121 aaaaaactga ttttgagaaa atcccgcttc gtcccttgac aggcgggatt ttccatgtta
    15181 aggtaaaggc agttatacga ctgacggaag tggaaatgac cacagggagt atagcgtgag
    15241 aagaggccga ccgtctgggc agaagaatgc ttgggggagt gggtctgttt ttttatccaa
    15301 aaacagaaaa tcccattcca gccaccttga aaaggagatc agaagatgaa aaaagtatca
    15361 ctggaacagg aattaaaagg aaatgcatat ccaggaagag gaattgtgat cggcagatcc
    15421 aaagacggca caaaagccgt tgccggatac tttattatgg gcagaagcca gaacagccgc
    15481 aaccgtgtct ttgttgagga gggtgaggga atccgcacac aggcgtttga tccgtcgaag
    15541 ctggaagatc cgagcctgat catctacgca ccggtacgcg ttctgggaaa tgatacgatc
    15601 gtaacaaacg gagaccagac cgataccgta tacgaaggac tggagcaggg aaaaacgttt
    15661 gaggtatccc ttcgaagcag agagtttgag ccggatgcac cgaactacac gcctcgtatt
    15721 tccggtgtga tgcacgtaga gaacggaacg tacggctacc agatgtcgat cttaaagagc
    15781 aataacggcg atccgtccca gtgcaaccgc tacaccttct cctatgataa ctgtgtagca
    15841 ggggaaggcc atttcatcca cacctacatg cacgacggca atccgctgcc aagttttgag
    15901 ggtgagccaa aactggtcga gattccggat gacatcgatg ccttcaccga actgctctgg
    15961 agcagcttaa acgaggacaa caaggtgtcc ctcttcacac gctatatcga cattgcaacc
    16021 ggcaagtatg agacaagaat cgtgaataaa aataaataag aaatcgagtg ttttgcgaaa
    16081 ggagaaaatc atgaaagaat tagcgttaaa atacggatgc aacccgaacc agaaaccttc
    16141 cagaatttac atggatgatg acagagatct tccgatcgaa gtattaagcg gccgtccggg
    16201 ctatatcaat tttctggatg ccttcaatgg ctggcagctg gtacgtgagc tgaaagcagc
    16261 caccggtctt ccggcagcaa cttccttcaa gcacgtttcc ccggcaggtg cagccgtagg
    16321 tcttccgctg gacgagaccc tgcgtaaaat ttactgggta gacgatatgg gagagctttc
    16381 tccgttagca tgcgcttatg caagagccag aggcgcagac cgtatgtctt ctttcggcga
    16441 ctttatcgca ctttctgatg tctgcgacaa agacaccgca tccctgatca aacgcgaggt
    16501 ttctgacggt gtcatcgctc cgggatttac cgatgaagca ctggaaatcc tgaaagcaaa
    16561 gaaaaaaggt aactactgcg tcatcaaaat cgacgagaat taccgtccgg ctccgctcga
    16621 gcacaaacag gtcttcggca ttacctttga gcagggccgc caggagctgc cgatcgacga
    16681 tgagctgctc tccaacgtgg taaccgagaa caaagagatt ccggaagcag caaaacgcga
    16741 tctgaagatt gcactgatta ccttaaaata tacccagtcc aactccgtat gcttcgtcaa
    16801 agacggtcag gccatcggtg tcggcgcagg ccagcagtcc cgtgtgcact gcacccgtct
    16861 tgccggacag aaagccgaca actggttcct gcgtcagtgc ccgaaggtgc tggatctgca
    16921 gttcgtagac ggcatccgcc gcgcagaccg tgacaatgcg atcgacgttt acatcggcga
    16981 ggagtacatg gatgttctcg cagacggcgc atgggagaag atcttcaaag taaaaccgga
    17041 agtcttcaca agagaagaga aacgtgcatg gctcgaccag atgaagggcg taaccttagg
    17101 ttccgatgcc ttcttcccgt tcagcgacaa catcgagcgc gcacacaaga gcggcgtcca
    17161 gtacatcgca gagccgggcg gatcggttcg tgacgacgct gtcatcgagt gctgcaacaa
    17221 gtacaacatg gcgatggcat ttacaggaat ccgtctgttc catcactaaa aagaaatgcg
    17281 ttttatattt atataagaag aaagaatccg gtctctgggc ggccggattc tttctttttt
    17341 cgcacatgcg gcaaaatatg catagtcgga atccgggttt ttgcgctata ctggacacgg
    17401 aggatttgtg aatgaaagca agagaaaaga tctggaaaat gatcaaaaca aaaattctgg
    17461 gggcgaaggt ccggtaccgc ctttttctgt cgaaaacccc tgttcttttt cggaaaagtg
    17521 tgataaaata ccccatgggc gtatcttttg cccgctgcaa aaaggaggat gaggaatatg
    17581 cggcagatgg aatttaaaat ggagcgacag ggacttctcg aggaaggcca ggaagtcaac
    17641 gtgacagaaa gcgcactgcc tacctcctat tattatacga ttaccccggc agtggccatg
    17701 agcaggaatt atcaggcgta tgagaggctg cagtcccgga agggaatcgt aaaagaagta
    17761 aaagagacac cgagaggatt ctatacggta gtggagtttg atgaagatga gccgacctga
    17821 cgagaacaaa aatgagcacc cggtggtgca tccgattccg ccggtttacc gcgccgattc
    17881 ccggattctg attctcggca gttttccgtc cgtaaaatcc agggagaccg gattttttta
    17941 cggccatccg cagaaccgtt tctggaaagt gacggcagcc gtgtgcgggg aagaggtgcc
    18001 gcagaccgtg gaagagaaga aggcgtttct gctccggaat catatcgctg tctgggatgt
    18061 gatcgcgtcc tgcgtcatca caggctcgag cgatgcgagc atccgcgatg ttgtgccgaa
    18121 tccgctgcag gacattctgg aatgcgccga tatccggcag atttactgca acggggcgac
    18181 cgcgtggaag tattaccgga agtaccagga gaagcagctg ggacgggaag cggttcgtct
    18241 tccgtcgacg agtcccgcga acgccgcatg gtctgtggag cggctgcttg gcgagtggag
    18301 ccagtgcgga gaagtcttac gaaacggaga gtgacaggat gaaaaaagag tacgagaccg
    18361 tctgggagat ttttaatgag tgtgccaata atcagatgcg ggacgtcttt ttcgacgaga
    18421 tcgagacgga tgacccggag gcgtatatcc gtcagaaatt tccggacaaa aacttaaaat
    18481 atgaaaaaac ggtggaagcg gacggatctc tggtctttga cattgagacc tccggcatcc
    18541 gccagcggtt tacgtttaca gaaatctgaa tcgcgcagaa aataccagaa aagcggagaa
    18601 aaaaacgatg agtgaagaaa aacacacgca tgtgcatgtg ctggaagacg gcaccgtgat
    18661 cgagcattcc cacgatcacg cccacgggca ccatcataca aatacaaaag cagtcttaaa
    18721 ccgcctctcc cgcgcgatcg gacacatgga gtcgatcaag cggatggtag aggaaggccg
    18781 ggactgcact gaggttctga tccagctgtc tgccgtgaag gcggccatta acaatacagg
    18841 aaaaattatc ctggaggatc acattgaaca ttgcatcgtc gatgcagtcg agcatgggga
    18901 cataaaagcg attcaggagc tgaataaggc gatcgaccgc tttgtcaaat aagcaagaaa
    18961 aacaagaaaa gaggaaagaa acatggcaaa agaatgcagc aattcgagct gttccaaaga
    19021 aagctgcgaa ggatgtccaa gcaaagcagg acagggcaaa ccggattttc atattgatct
    19081 gaatgccgat tcttcgatca aacatgtcat tggcgttgtc agcggcaagg gcggtgttgg
    19141 taaatccatg gtaaccgcat ctctggcaaa ccagaaagca aaagagggct acaaggttgg
    19201 tatcctcgat gccgatatca caggaccgtc catcccgaaa atgtatggcc tgaaaggacc
    19261 tgcaaacgta gatgacaagg gcgtttaccc ggcagttgca gcaaacggcg taaaggtaat
    19321 gtccatcaac ctcctgcttc cgggcgagga agagccggtc atctggagag gacctgtcat
    19381 cgcaggaacc gtaaaacagt tctggaccga cgtcatctgg ggcgacctgg actatctctt
    19441 cgtcgatatg ccgccaggaa ccggtgatgt tccgctgacc gttttccagt ctcttccggt
    19501 agacggcgta gtgatcgtat cttccccgca ggatctggtg cgcatgatcg tcatgaaagc
    19561 ctacaagatg gcacagatga tgagcgttcc ggtgctcggt atcgtagaga actacagcta
    19621 cctcgtatgt ccggactgcg gcaaaaagct gtccattttc ggcgaaagcc acatcgacga
    19681 gatcgcagcc gagatcggcg tgccggttct cggcaaaatg ccgattcatc cggagtttgc
    19741 cgagctggtg gacgaaggaa gatttgcaga agtggataac accgatattg caaagatcaa
    19801 catctaatca ttaaatatag aagcaagtaa tggcatgaaa aactcgtttt tccttgattt
    19861 tacaaggaaa agcgggtttt tttgacaaga tttttacgtt tcttttggca aaaactattt
    19921 tattttaaac ggattttgtg taaccggtta cacacgactg cgcaaatgag acaaaaagcg
    19981 acaaatatga agggttaatg agaaaaactt gcataaaaaa catgcataat atggcaaaaa
    20041 caaggaaata aattcaatat tgacatttaa agagaaaaag tgcataatct acctcaacga
    20101 taggaaaaca aacagctatt gattaagaaa gaggtgaatc gtatgtcaca ctgcgtagta
    20161 agactcaatg gaactgagga agtagaggag tttgtaaaag cagcttccaa atgtgatttt
    20221 gatattgaca ttttttacaa ccgcgttatt attgatgcaa aatccattct tggcatcctc
    20281 agcatggatc tgaaccgtga tctcaccgta cactgttatg gtgacagtcc ggcattcaat
    20341 aaaaccctgc agaaatttgc tgtagcataa attttattaa gggaaacgaa aaaaattcag
    20401 cctgcgccgc cagcgagagt agagacagaa tttgcgcagg ggactttctg aagaaaaact
    20461 aatcacacga gacacataac ttcagagaga aaaaaaggag actgttgcgg cagtctcctt
    20521 ttttctgtat aatgataact attcagagcc atgcgaaatt ccagaaaagg acacgaatca
    20581 tgcttgcatg aatgagcgtt cttttctgga atttcatatg gcgagaagcc acatcgagat
    20641 gcagcgcagc ggaatcgaga tggggctctg aatagtctgg ttagaaaagg agcgggctag
    20701 tggaagagaa gaagaaagag atccggattt ccgtgcggaa tctggtggag tttattctgc
    20761 gcagcgggga tctggacaac tcgtcgggag gttttggcga gcgcgatgcc atgcaggcgg
    20821 gaacgcgggt gcatcagcag attcagaaaa aacggggcgc cgattaccgt gcggaggttc
    20881 cgctggttca cgaaaaagaa tatgagcatt tcatcctgcg cgtcgaggga agagcggacg
    20941 gcatgtacga ggacggaacc acgaccgtga tcgaggagat caagggaacg taccgtgacc
    21001 tcgaaacgat ggaagagccg gttccggtac atctggcaca ggcgaagtgc tacgcttaca
    21061 tctacggcct tcagaaccgc aaagaggaga tgggcgtgct gatgacgtac accctgctct
    21121 cctccgagga ggaaggcctt ctgcgcccgc tcaccaaaga agaggtaaag ccggagcatt
    21181 ctaactggcg catccgccat tttctgcaga cgtacaagct cccggaactg gagcagtggt
    21241 tcgatgagct actcgacgcc tggtttatct gggcggaatt ccagtaccag tggcagaaag
    21301 cgcgagatgc ctcaatgcag cacctcgaat tccccttccc gtaccggaaa ggccagcggg
    21361 aactggtgag cggcgtctac cgtaccattc tgcggaaaaa agaactcttc gtccaggcgc
    21421 cgaccggcat cggaaaaaca atgtccacgg tctttccggc catccgcgcg atcggagagg
    21481 gctgcggcga ccgtcttttt tatctgactg cgaaaaccat cacccgtacc gtggcagagg
    21541 aggcggtctc cattctaaaa gagaagggac tgcagttcaa agcaatcacg ctgaccgcaa
    21601 aagaaaaaat gtgcatcctc gatgagcccg aatgcgatcc gatccactgc ccgcgcgcga
    21661 aaggtcattt tgaccggatc aacgcggcgg tgtacgagat gctgacggaa tgcgattcct
    21721 acacaagaga ggaagtcctg cgccaggcgg aaaaatggaa cgtctgcccg cacgagctcc
    21781 agtttgatgt ggcttcgtgg gtggacggcg tgatctgcga ttacaactat gcgtttgatc
    21841 cgacctcgaa attaaaacgc tttttcgcag aaggaacgaa gggtgactac atctttctga
    21901 tcgacgaggc gcacaacctg gtcgagcggg gccgcgatat gttcagtgcc acgctcgtca
    21961 aagaggagat tttaaaggtc cgccagctcc tccgcccgta tgcaccgggc aaaaagctcg
    22021 agaaggcgct gaaccgctgc aatcaccaga tgctcgtcta caaaagagag tgcgattcct
    22081 gcacggagct ggaaagcgta tcgacgtttc ttatgatggt caatcagctg ctggaagaac
    22141 tggcgggctg gctggaagcg cataagacaa gcgaaatccg gaagcaggtt ctggaatttt
    22201 actttaactt gcgaaatttt tccgagattt acaatttagt ggatgaaaat tatctgatct
    22261 acacgagtta tctcgataac ggcgattttg cactgcgcct gttctgcgtc aacccggcgg
    22321 agaatctgca gcagtgtatc aaccaggggc ggtcggccgt cttcttttcc gcgacgctgc
    22381 tgccggtgca gtattataag aagatgttca gcacgaatac ggacgattac gcgatttacg
    22441 tggagtcccc gtttgatccc acgaagcggt gtctggcgat cggaagcgag gtcagcacga
    22501 agtatcagcg gagaaaccgc gcagagtttg agaagatcgc cgcctatctg aacgagatga
    22561 tccagagccg gaagggcaac tatatggcgt tcttcccgtc ataccgcctg atgcaggatg
    22621 tctatgcggt ctatgaggaa ctgtatgcag atgaaaacgt gacctgcctg atccaggagt
    22681 cggcgatgcg ggagcaggag agagaagagt ttctggaagc gtttgcgaaa gacaacgaga
    22741 aaacgctggt gggattctgt atcatgggag gcattttctc ggagggaatt gatctggacg
    22801 gggaaaagct gatcggcgcg gccgttgtgg gagccggaat cccgcaggtg gggccggaga
    22861 gagaactttt aaagcaatat ttcgaccgca gcggagagaa cggtttcgac tacgcgtacc
    22921 gctatcccgg catgaataaa gtattgcagg cggcagggcg cgtgatccgt acgacggagg
    22981 acacgggtgt gattctgttg ctcgacgagc ggttccgaaa ccgggagtac cgcgagctgt
    23041 tcccaagaga gtgggaggac tgccggattg tgcagcgcag cagcctcccg gaggtaatcc
    23101 attcgttttg ggagagggtg gattgtgaga gcggatcaaa agccgggcag caggaaatga
    23161 aatctcggta cagcgctgaa cgttacgtgt gatccgcgcg gttccggtat tcgctcggtg
    23221 agcagcccat ctgctgccgg aaaattttag aaaaataggc aggactggaa aatccggtct
    23281 gttccgctac ggaagtgata ctggcctggc gctcgagaag gagcggcagg cttttttcaa
    23341 tacggtaatc gagaagatag tcaaacaggg attcgttcat gcatttcttg aaaaaacggc
    23401 agcattcact gcggcagatg tggatctgtg cggcgagatc ctcgagcgtc agatgctcca
    23461 tataatggcg gtggatgtag tcgagcagaa tgcggagccg ctcaacatca cgcgctgccg
    23521 ccgtgttctg ggattcaccg gaaaacgatg cgtggcggta aatctcaatc cagatcgaca
    23581 gcagaagctc ctggatttcc atctcaaacg cggacggctg cgaaagcgag aggtcgtaga
    23641 tgcggtgcat acaggcaagt acctggttct gccagtcgat ctccggcgaa aagacgatgg
    23701 aggcgagctg catatccgtc agcaccggtt cgacgtagcg cgtctggatc acactgtttg
    23761 aaaaaccata gagaaagcgc ggggcgaacg tggttgaaat atagacgcag tccatcccgt
    23821 cgatcatgtg gccgctgtga agcgcattcg tgttgcagaa aatgccgtcc ccttctttta
    23881 agacatacga tttgccgttg acctgatatc cgatctgccc cgagaggaca aacgtcagct
    23941 caatttccgg gtgccagtgc caggaaaagg agccgcgctc gtagcgggag agctgttcgt
    24001 aactgatatt gacaggaaat tcatagctgc cgtgggcctt cagctcacgg ttttctttat
    24061 ccatgcggat ctgcatgaaa cgtacctcca atccataaaa tccgttaaaa atcatacgat
    24121 aagtaaagta tgcggcaaaa agggaaaaaa aacaagaaaa aatcgaagat cacctcaata
    24181 taattatcgt ttgccccaat attgttgttg cagatgggcg tacggctcac tataatgata
    24241 cttaagaaga taagaaaaaa cacacaaaca cacaaacaca caatacacac acaacacaca
    24301 aaacacacaa aacgaaacca cgaaaacaac acaccaaaaa gaaaaggaga caacaaaatg
    24361 aaacagcagg tactggattg ttttgcaaaa ctgttcgggg gagagggcga catccgcacc
    24421 tacttcgcac ctggacgtgt gaacctgatc ggtgagcaca ccgattacaa cggaggccac
    24481 gtatttccat gcgcactgac catcggaacc tacatggcag caagaaaacg tgacgacaga
    24541 aagcttcgtt tttactctat gaactttgaa aaactcggcg tggtagaatc ttcgctcgat
    24601 gagtttcaga tgggcaaaga aggcaactgg accgcatatc cgaagggaat gatgtgggca
    24661 ttttgccaga agggcttccc ggtagagcag ggcttcgata tcgtgctcta cggaaacatt
    24721 ccgaacggct ccggcctttc ctcttccgca tccgttgaga ccgtaaccgg cgtaatgctg
    24781 cgtgacatgt tcggctacga caccatctcc atgatcgaca tcgctctgta cggccagttc
    24841 tccgagaaca actacaacgg cgtcaactgc ggtatcatgg atcagttcgc catcgccatg
    24901 ggcaaaaagg actgtgcgat cttccttgac accagcgatc tgaaatatga gtacgcaccg
    24961 gttaaactgg aaggcgcaag aattgtcatc tcctgcagca acaagaaacg cggccttggc
    25021 gactccaaat acaacgagcg ccgcagcgag tgcgagactg ctctggctga gattcagaaa
    25081 gtcaaagaca tcaagagcct tggcgagctg accgaggaag agtttgaggc agtgaaagat
    25141 gccatcaaag atccggtccg ccagaaacgt gcaaaacatg ctgtttacga gaaccagaga
    25201 accgttcagg ccgttgcagc tttaaaggca aacgacatcg caaaattcgg cgagctgatg
    25261 aacgcatccc acgtttccct gcgtgatgac tatgaggtaa ccggcatcga gctggatact
    25321 cttgtggaag aagcatggaa ctgcccgggt gtcatcggat cccgtatgac aggcgcaggc
    25381 ttcggcggat gcaccgtcag catcgtaaaa acagaagata ttgacaactt catcgcaaaa
    25441 gtaggcgcag cttacaaaga aaaaatcggc tatgccgcag acttctatgt cgtagacatc
    25501 ggagacggcg caggcgtatt aaaataagaa ataaaaaagc tggatcggca gcagggagga
    25561 agctacagtg atcaacgaga gtattgcaaa actggtaaaa tatggagaaa atgccggtct
    25621 tgtttcggga ctggataaaa tctatgcgac aaaccggatt ctggaagtca tgcagatttc
    25681 cgattacgaa gagccggagc agattccgga gaccccggat ctggaagaga ctttaaatga
    25741 actgcttgat gatgcagcta agcgcggtct tctcgaacac aacagcgtcg tttaccgcga
    25801 tctgttcgat acgaaactga tggactgcct gatgccgcgc ccgggagagg tggtaaagaa
    25861 gtttgaagaa ctctatgcga agagcccgca ggaggcaacc gattacttct acaaactgag
    25921 ccaggattcc aactacatcc gcagataccg catcgcaaag gatatccgct ggagcgtacc
    25981 gagtgcctac ggagatatcg atatctcgat caacctctcc aaaccggaga aggacccgaa
    26041 ggcgatcgcg gctgcaaaac tggcaaaaca gagcggttat ccgaaatgcc tgctctgcaa
    26101 ggagaatgtg ggctatgcgg gacgcgtcaa ccacccggcg cgccagaacc accgcatcat
    26161 tccgctgacg atcaatcaga cggagtgggg attccagtac tcgccatatg tgtattacaa
    26221 tgagcactgc atcgtgttca atttccagca taacccgatg aagatcgagc gcgcgacctt
    26281 tgtgaagctg tttgacttta tcaagctgtt tccgcactac ttcatcggtt ccaatgcgga
    26341 tctgccgatc gtgggaggct ccattttaag ccacgatcat tatcagggcg gccactatac
    26401 cttcgcaatg gcgaaagcgc cgatcgagcg ccattttacc atcaaaggat acgaggatgt
    26461 ggaaaccggc atcgtcaaat ggccgctgtc cgtcatccgg atccgccaca aagatgagaa
    26521 acggctgatc gatctggcag agcatatcct gaactgctgg agaggttaca cggatgagga
    26581 tgcgtttgta ttcgccgaga ctgacggaga gccgcacaac acgatcacgc cgatcgcaag
    26641 aaaagtgggc gatacgttcg aactcgatct ggcactgcgc aacaacatca cgacggacga
    26701 atgcccgctc ggtgtttacc atccgcatgc acagtatcat cacatcaaaa aagagaacat
    26761 cggccttatt gaggttatgg gacttgcgat tctgccggcg cgcttaaaag atgagctgga
    26821 agagctttcc aaatgcctcg tagagggaaa agatgtccgc gctgtcgcag agctggagaa
    26881 acatgccgac tgggtagacg aattcctgcc gcgccatccg gagctgaaca gcgaaaatgt
    26941 gatggatatc ctgaaagagg aagtcggaaa agtctttgtc ggcgtactcg aggatgccgg
    27001 cgtttacaag tgcaccgagg aaggcagaag tgcatttgca aaattcatgg aaacgttgta
    27061 gaataaacta aatgcttcaa ttaacccgct gcggcaaaaa agcagcagcg aaggaaaaca
    27121 tggaggatac caaaatgaaa atcttagtta ctggcggtgc aggctatatt ggaagccaca
    27181 cctgtgtgga actgctgaac gcaggctatg acgttgttgt catggacaac ctttacaatg
    27241 caagcgagaa agccatcgag cgtgtagaac agattaccgg aaagaaagta accttctaca
    27301 aaaccgatat gctggaccgc gaaggcgtaa agaaaatttt cgacaacgag aaaattgacg
    27361 ctgtcatcca tttcgcaggc ttaaaagcag tcggcgagtc cgtacataaa ccgattgaat
    27421 attatcacaa caacatgacc ggaaccctga ttctctgcga tgagatgaga aaccacggcg
    27481 taaagaacat catcttcagt tcttctgcaa ccgtatacgg caatccggca cagattccga
    27541 tcaccgagga gtgcccgaag ggaaccccga ccaacccata cggctggaca aaaagcatgc
    27601 tcgagcaggt tctgaccgac atccacaccg cagatccgga atggaatgtt atcctgcttc
    27661 gttacttcaa cccgatcgga gcgcataaga gcggccttat cggagaggat ccgaagggca
    27721 tcccgaacaa cctgcttccg tatgtcgcac aggttgccat cggaaaactc gagtgcatcg
    27781 gcgttttcgg tgatgactac gatactccgg acggaactgg tgtccgcgat tacatccatg
    27841 ttgttgacct ggcacgcggc catgtaaaag ccatccagaa actggctgac aacgagggcg
    27901 tttccattta caacctcgga accggcaaag gctacagcgt actcgatgtg atccacgcat
    27961 tcgagaaagc atgcggccat ccgctgaaat acgaaatcaa accgcgccgt gacggtgata
    28021 tcgcaacctg ctactccaaa tgcgacaaag caaaagagga gcttggctgg gaggctgagt
    28081 acggcatcga ggagatgtgt gccgattcct ggaactggca gcagaagaat ccgaacggat
    28141 ataacgacta atgtaatgtg agtgttatct ggcggtccgc cgatacttga aagagtgtcg
    28201 gcgggccgtt tttgtataga taaattcagt cttttttcac aaagtccggc ttgctaatta
    28261 agagggaatc cgatataatg taagagttaa agtgtccgta aaaaaagagg ggcggtttga
    28321 catacaagga ggaaaagcat ggacgataga aaaaatcaga accgggaacc agaaaacggc
    28381 tccgggggag gaccgaaaaa cagacagacg atactcatta tcctgatctg cctcctcgtc
    28441 agcctgatgg tgatgggact tctgaacaat gcccttgggg gaattacttc cgaggatctt
    28501 gactatagca aattcctgga catggtaaac aacgatgaag tgcaggaagt tgtcttgaaa
    28561 tcgggaacac tgaccgtaac gccgaaagac cagaaatcgg agggaatcac ctatcaggta
    28621 acgatgatgg gagatgagca ggagctggtc gatctgttac aggagaaagg cattacgtac
    28681 cacaaagatc cgccggattc taccggaacg atcatttaca cattgctgac cgtcattctg
    28741 ccggttgtga tgcttgtcgc tgtcatggta tgggtgatgc gcagcatgaa caaaggcggc
    28801 ggagtcatgg gcgtaggaaa gagcagagca aaggcctacg tacagaaaga aaccggcgtg
    28861 acattccgcg atgttgcggg acaggacgag gcaaaagaat cgctccagga ggttgtggat
    28921 ttcctgcata accctggaaa atacacgaca atcggtgcaa aacttccgaa aggagcgctt
    28981 cttgtaggcc ctcctggaac cggaaaaaca ctgcttgcca aagccgtagc cggtgaggcg
    29041 cacgtaccgt ttttctcgat gtccgcttcg gaatttgtcg agatgttcgt cggtgtcggt
    29101 gcatcgcgtg tgcgtgacct gtttgaggaa gcaaagaaaa acgcaccgtg catcatcttt
    29161 atcgatgaga tcgatgcgat cggaaagagc cgtgacaaca gctacaacag caacgacgag
    29221 cgggagcaga cgttaaacca gctcctcgcc gagatggacg gattcgacag ctccaagggt
    29281 attctgatcc ttgcggccac aaaccgcccg gaggttcttg acccggcgct gctgcgtcca
    29341 ggccgttttg accgccggat tatcgtagaa agaccggacc tgaaaggccg tgtggaaatc
    29401 ctgaaagtac acgccaagaa cgtatcgctc gatgagacgg ttgatctcga cggcatcgca
    29461 cttgcgacat cgggagccgt tggttctgat cttgcaaata tgatcaacga ggcagcgatt
    29521 cttgcggtca agaacggaag aaaagccgtt tcccagaagg atcttctcga ggcagtcgag
    29581 gttgtcctcg tcggaaaaga gaagaaggac cgcatcttaa gccaggaaga gcgaaagatc
    29641 gtttcctacc acgaggtagg ccatgcactt gttactgcac tgcagaagga ttccgagccg
    29701 gtacagaaaa tcacgattgt gccgcgtacg atgggtgcgc tcggctacgt catgcaggtg
    29761 ccggaggagg agaaatacct caacacgaag agcgaactcg aggcgatgat cgtcgagtgc
    29821 ctcggtggaa gagcggcaga ggagatcgta ttcggcaacg tgacaacggg agcttcgaat
    29881 gatatcgaga aagcgacgaa gattgtccgc gcgatgatca cgcagtacgg tatgtcggag
    29941 aaattcggtc tgatcggtct tgcgacccag cagaatcagt acctcgacgg aagaatggtc
    30001 ttaaactgcg gcgatgccac agcaaccgaa atcgaccacg aggtcatgaa gatcctgaaa
    30061 gagtcgtatg agcaggcaaa agaacttctg gcagctaacc gcgatgcgat ggatgaaatc
    30121 gcagcttacc tgatccagaa agagacgatc accggaaaag aatttatgga gatttttcat
    30181 caggtgattg caaaacggaa tggaaacgcg gaagaagcgg ttgaaaatcc agaaaataca
    30241 gaaacagaaa atacagcact tccggaggcg gattttaccg taaatccaga agtataaaaa
    30301 gaaagcgaaa aggcccggcg ggagcagaga aaacgatctc tgcacgctgg gcattttcaa
    30361 cattacagtc ccaaaaagag gagggtcata tggcgtttga tatccaggaa gaactgaaaa
    30421 aactgccggg gaaacccggc gtctatctga tgcacgacgc ggacgataca attatctacg
    30481 taggaaaggc gatttcgctg aaaaaccgtg tccgccagta tttccagacg agccgcaaca
    30541 aagggccgaa gattgagcgg atggtcacgc atatcgcccg ctttgagtat attgtgacgg
    30601 attccgaact ggaggcgctc gtgctggagt gcaaccttat caaagagcac cgtccgaaat
    30661 acaatacgat gctgaaagat gacaaggcgt acccgtacat taaggtgacc gtgaacgagg
    30721 attttccacg cattctgttt tcccatcaga tgaagaagga caaggcgaaa tatttcggcc
    30781 cgtacacgag cgccggagct gtgaaagata cgatcgaact gttgcggaag ctgtacgata
    30841 tccgttcctg caataagagc ctgccaaagg agatcggcaa ggaccgcccg tgtctttact
    30901 atcacatcca ccagtgtaag gcgccgtgcc agggatacat ttccaaagaa gagtacggcg
    30961 agcagatcaa aaaggcgatc agttttctga atggaaatta caatgacatt atcaaagagc
    31021 tgactggaaa aatgacggag gcggctgagg agatgcggtt cgagcaggcg gcggagtacc
    31081 gtgatctgat cgacagcgtg cgcaggatcg gcgagcggca gaagattacg aacagtgacg
    31141 gcgaggacaa ggatgtcatt gcgcttgcga tggacaaaga ggatgcggtg gcgcaggtct
    31201 tctttatccg gaacggaaag ctgatcgggc gggagcatta ttatctgcgg atcggcgagg
    31261 aggagcagaa aagcgaggtg ctgctcacgt ttatgaaaca gttctattcc ggcacgccgt
    31321 tcatcccgcg ggaaatcatg atttccgagg agattgccga gcaggagctg atggaagagt
    31381 atttaagcag caaacggggc cagaaggtcc gcatccgcgt gccgaaaaag ggcagcaagg
    31441 aaaaaatggt ggaactggca gagcggaacg cacagatcgt tctcgaccag gaccgcgagc
    31501 ggatcaagcg ggaagagggt cggacggtcg gtgccgtaaa agagatcgcc agatggctga
    31561 acctgccgtc gatcgaccgc atcgaggcgt acgatatctc caatatcagc ggtttccagt
    31621 cggtcggctc gatgatcgtc tacgagaaag gaaagccaaa gcgcgccgat taccgaaaat
    31681 tcaagatcaa atccgtcgag ggaccgaacg attacgccag tatgaaagag gtcttaagcc
    31741 gccgttttct gcgcggcatc caggaggaca gcggtttcga gcggctgccg gacctgatca
    31801 tgatggacgg cggacgcggc caggtcaaca ttgcgcttga ggtgcttgaa gaaatggatc
    31861 ttgcgattcc ggtctgcggt atggtaaagg acgacaagca ccgcacgagg ggtctgtatt
    31921 tccgaaacgt ggaaatcccg atcgaccgga acagcgaggg cttcaaactg atcacacgcg
    31981 tgcaggacga ggcgcaccgc tttgcaatcg aataccaccg cctgctgcga agcaagggcc
    32041 aggtccactc gattctcgac gacatcccgg gcatcgggcc gacaagaagg aaagaactga
    32101 tgcgccactt tcagggaatt gatgatatca aggcggcaac cgtggaagaa ctttccggac
    32161 ttccgggcat gaataaaaag gcggcggagc aggtttacac attctttcac gcacctgcac
    32221 aggaggaaaa tgaggattcg tcaaaatagt taaatttcta ggttgcccgt gatgctttat
    32281 tgtgctaaaa tcatagaaga gacggattta aaaagacgtc gaatgaagtc ggaggacagg
    32341 aaaatgaaag aaattgaaat gaccaaaatg gtggaggaaa tggatctccg caacctgaca
    32401 ccagaggttg atatgacaga tcttgtcatt gccacaccgg atatcaaccg tcctgcactg
    32461 cagctgaccg gttactatga gcattttgcc aatgagcgtg tgcagatcat cggttatgta
    32521 gagttcacgt atctgcagca tcttgaagag agcgagcgcg tccgcgcgtt tgaagagttt
    32581 gtaagccgca agatcccatg tgtcattttc accacgaaga tgattccggg accggaaatt
    32641 ctggagctgt gcaatcgtta tcaggtaccg gcctttgcgg tagacaaatc gacttccgtt
    32701 ttcatggctg aaatcatccg atggctgaat gtccagcttg ccccgatgat ctccatccac
    32761 ggcgtgctgg tcgatgtatt cggcgagggc gttctgatca tgggcgagag cggaatcgga
    32821 aagagcgagg ctgcactgga gctgatcaaa cgaggccatc gtctcgtcag cgatgatgtg
    32881 gttgaaatca gccgtgtcag cgacattacg ctggtcggaa cggcaccgga tatcacgcgt
    32941 cactttatcg agctgcgcgg tatcggtatc atcgacgtca agacgctgtt tggtgtcgag
    33001 agcgtaaagg atacccagtc tatcgacctt gttatcaagc ttgaagagtg ggacaaagag
    33061 aaagaatacg accgtctggg cctggaagaa gagtatattg agttccttgg aaacaaggtt
    33121 gtctgccatt cccttccgat ccgtccgggc cgtaacctgg cgatcatcgt agaaaccgct
    33181 gctgtcaacc acagacagaa gaagatgggc tacaatgccg cacaggagct gtaccgccgt
    33241 gtacaggcta acttagtgaa aaaaagagag gagaagtaag aaaatggcaa actatgtatt
    33301 tggaatcgat gttgggggta cttcagtaaa atgcggactg ttccagacag acggaactct
    33361 gttggaaaaa tgggaaatcc cgacccgcac cgaaaacggc ggaagcgaaa ttctgccgga
    33421 tatcgcaaaa agcgtaaaag caaaaatggg tgagaagggc atcgcagcag atgatgttgc
    33481 cggtgtcggc atcgacgtac ctggcccggt taatgacaaa ggtgagcttt ccatcgcagt
    33541 caacctgaac tggggatata aaaatatcgt aaaagagctt tccgacgaac tcggcggaat
    33601 ggcagtaaaa gcagcaaacg acgcaaacgc agcagctctc ggtgagatgt gggcaggcgg
    33661 cggaaaggga agcaagaacc tggttatggt tacccttgga accggtgtcg gcggcggaat
    33721 catcgtggac ggcaagatcg tagcaggtgc tcacggcgca ggcggcgagg ttggtcatgc
    33781 atgtgttgat ccggaagaag aagcggtctg caactgcgga aaccacggat gcctcgagca
    33841 gatgacatct gcaaccggta tcgttcgtct ggcaaagaaa tatctggcat cccatgatac
    33901 tccgtcttct ttaagagaaa gaggagagtc catcagtgca aaagctgtct ttgatgcatt
    33961 aaaagaaggc gatgcagcag cagaagcaat cgttcaggag ttctccgcgt acctcggaag
    34021 agcacttgca gtattcgcat gcgttgttga cccggaagtt atcgtagtcg gcggcggtgt
    34081 atccaaagca ggccagatcc tgatcgacgg cgtagctaaa tactaccgtg aagcagcatt
    34141 catcgcatgc aaagataccc cgatcgtact cgcttccctt ggaaacgatg ctggtatcta
    34201 cggcgcagca aaaatgctga ttgactaatt gattcagatg gaaaaagaag tatagaaaaa
    34261 ccggtcctgc tggcatatgc cagagggccg gttttttgtg catagactga ctgaaaaagc
    34321 tattcggaag agccgctctc ttcggactgc ggctgtactt ccgatgtctc cgccggggta
    34381 tccggttctg acggtgcatc aggggtatcc ggtgtgtcgg gagtttctgg tgtattagaa
    34441 ggcggatcgg caggtgtttc gggcggagcc ggagtttcct gcggtgcctc cgtcggtgtc
    34501 ggggttgcct gcggagttga tgctgcagaa ggggttctgg tcggcgtcgg tgttgaggtc
    34561 ggcgcggtgt agtggccctt gcaacgctcg gtcggcagat cggataccgc aaagtattcg
    34621 gtgatggacg ggcagcttga gcgggcgagc aggccggtct tcgtacagat cgttgcctcc
    34681 tgtactgttg cggaagtttc gaaatccata tcggcaaggc cttcgtggat ttcattcatg
    34741 acctttgccc agagacgttt gtggaattca cggtcgctct ccggaaactc cgcgtgtgta
    34801 tcatacgttg cccagatggc agcggtataa tatggtgtaa agcctgcaaa gacgagatcc
    34861 ttggcctcgt tcgtcgtacc ggtttttccg gctgcgtgca tattgtcgag gcgggcggca
    34921 acaccggtac ctttttccag aacatcctcc atggcattgg tgaggaggga ggcagtgctt
    34981 gccttgaaga cctgcgttgc cttgttttct gtgttgtcaa ttaaaacatt tcccttgctg
    35041 tcggtaacct ttgtgtagaa caccggcttg cggtactggc cgccgtctgc gattgccgcg
    35101 taggctgccg tcagttcgag atcactgaca ccgtaggtga taccgccgag agccagcggc
    35161 tggatcacat cgtattcctc ggagagtttg gaaaaaccaa gcttcttcag ataatcaaag
    35221 ccgaccttcg gcgtaagctc ggtcagtacc ttgacggccg ggatattgat ggaattgatg
    35281 atcgcctggc ggacggtaac atcaccatgg taggtaagat ccgagttctg cagcggtgtg
    35341 ccgtcgctgt agttgaacgg ctcatcctta attaccgtag cgagtgtaat ctttccaagg
    35401 tcgagcgccg gtccgtacgc ggaaagaatc ttaaacgtgg aacccggctg ggaataatta
    35461 tcggttgcgc ggttgaaggt aagacttgct gttttttcgc cgcgtccgcc gacgatggct
    35521 ttcacgtaac cggtgcgctg atccataacg gtcatgcacg cctgcggctg cggggtgaag
    35581 ctgctgcgct cggcaacaat cgtatcgccc tcctgcatga tagcggcttt gtactggtcg
    35641 atatagctct gtgcctcctc ctgggagtca aagagcagat cgaaattcgg atcgctgttg
    35701 cggaagtaga gctgcagcat ctctttgctg tagttctgta cctcgccgtc tgcctgctct
    35761 acggtgagcg cccagtcgag accgatctgc gtgttcgccg ggaagttctc cggattctgg
    35821 aattccgtat cgagaatcga ctgcatgtag gaatcctgtg tggtatagat tttgaggccg
    35881 ccgctgtaga caacgttctt tgcctgcact tcggtgtagc ctttctgtac catcagatcg
    35941 ttgatcaggt tggtgatgac ttcatcgata aagtaggagt acggcgcgct ctccgcctgg
    36001 gactgcgtgt gcatggcgat gcggtcgtaa acattgtcgt tcatggcaat ggtgtattct
    36061 tcttcggtga tgtagccctg ctctttcatg ttgttcagga tcttgtcgcg gcgctgctgg
    36121 tttttctccg ggtggttttt cggattatat gccgtcggcg cctttggaat tgcggcaaga
    36181 acggcgcatt cagagagcgt cagatctttt gcatcctttc cgaaatacgt ctgcgctgcc
    36241 gtctggacac cgtagcagcc ggaacctaag ttgatcgtat tcagataatt ttccagaata
    36301 acgtctttgg cgttttcacc ctctgcggtc agtgtttttt ccagcttcag cgccaggtac
    36361 tgttcctgga tcttccgctt gaagctctgc atccgcgtct cttccatcca gtcagtgaaa
    36421 acattgtttt ttaagagctg ctgtgtgatc gtgctggcgc cctcggtaaa atgaaagccg
    36481 tggctgacgc cgacaacgaa agcacggatg ataccgctcg gatcaatgcc gttgtgctca
    36541 taaaaacggg catcctcaac cgcgacgatc gcgtgctgca tatcgagcgg aatctcctca
    36601 atcgaaatcg agacacggtt tccctgagcc ccggtcagct tctgtacctg gttgccgtca
    36661 gcatcgtaga taaacgacgc gttgccgagc ggcatgatat ttgcctccga gatatcaggg
    36721 gcactgtcaa tcacgccgcg gacagcgccg atcacaagcc cggtaccgca gacgagaacg
    36781 acaagaagaa gacagaacgc gatgcgcagc gccgtcacgc cggccgtctt ttcaatcttt
    36841 ccgtaatggg accgcagatc ttcacgtttg tcttccagat gactttttcc aaagtgcata
    36901 tagaaaatcc tccttatgtc atgttcctgc aaaactgatt cagttcagta atcaatggtg
    36961 ttcctgccct gcaaaaagca gggtatactt atttttatta taccaaacaa agggctggaa
    37021 taaaagtatt tctgaaaaga atgctgattt ttcagaaatg atatggtaag ataggtacat
    37081 atttggatga aggagtaagg acatatgatc gatcagtttc ggacggttta ccggggtgga
    37141 accggggaaa tcgtggagaa gaaatcacgt tttattgcga cggtgcgtct ggtggaatct
    37201 gaggaggagg cactttcctg cctggaagct ctgcggaaaa aatactggga tgcgcggcat
    37261 aactgttttg tgtatattat tggagaaaat caggagacgg tgcgctgcag cgacgacggg
    37321 gagccttccg gcacggcagg gcggccgatg ctcgatgtgg tacagggagc aggcctccgc
    37381 aatgtgcttg ttgtggtgac gcggtatttc ggcggtacgc tgctgggaac gggcggcctg
    37441 gtgcgcgcgt actcgcaggc ggtgcaggag gggctggcaa acagtgttct gattgacgag
    37501 atctgcggcg tgcggctgtt gattgagacg gattacaacg gcatcggtaa aatccagtat
    37561 ctgctcggcc agcgggggat tccgattctg gaatccgagt acaccgatca ggtgaaaatc
    37621 cgcgttctcg ttccgaaaac ggaggtggac cgtctctgcg cggagattac cgaggggacg
    37681 aacggaaagg caaaactgga aaaagaagag gagctgtact ttgcgcagaa cgacggagag
    37741 ctgatcttcg gggatgagct gacgcagtat aaaaatctgg aggcagaaga attaggataa
    37801 aggaggatgc gacatgaggt ttccggaaaa tctgaaaaag ggcggaacga tcggctttgt
    37861 ggcaccatcg tttggatgca caacagaacc atacaagtcc gcgtttgcaa atgcgcagaa
    37921 aaaatggaaa gagatgggat atcagctgga tcttgggcca aactgctatg cagagcacgg
    37981 catcggcatc agtgcggcgc cggaggagtg cggaaaagaa ctgacggaat attatgtcag
    38041 tgagaagaat gactgtctga tatcctgcgg aggcggagag ctgatgtgcg aggatctcga
    38101 ttacgtggat tttgaaaaga tcagaaaggc accggcgaaa tggtatctcg gctattctga
    38161 caatacgcat tttacgtatc ttctgccgac cctctgcgat acggcggctg tttacggtcc
    38221 gtgtgcgagt gatttcggca tggagccgtg gcataaatcc gtagcggatg cgtttggcgt
    38281 actgacggga gaagtgcgta ccgtccgcgg ctatgaggga tgggaaagag acgatgtgaa
    38341 gggcagaagc gaggaaaatc cgtatctgcc gtaccagatt acagaactgc gcgttctgcg
    38401 ccgcttcccg gatgcggaca gtgcatttga gggacggctc atcggcggct gtctcgactg
    38461 tctcgtcaat attcttggaa cgaagtacga cggaacggtt gattttgtgg aaaaatataa
    38521 agaggacggt tttgtctggt ttctggaagc gtgcgatctg aacgtctttg cgatccgccg
    38581 cgcaatctgg cagatggcgc ccgctgcagt tcggacagaa attcggggaa ctcgaccagt
    38641 accgcgccgt gacggatctg cttggaaaat acaacgtgcc gatcgtgatg gatctcgaca
    38701 tcggacacct tcatccggcg atgccgctga tcgtcggaag ttatgcgaag gtggccgtgg
    38761 aaggaaatga gattgaaatt aagatggagt gccgttagaa cgcttttgaa aaatattttc
    38821 tcgagcggca gggcagagcc tgtgggaaaa tgtttgcgcg cgtagaagaa ctatcggcgc
    38881 atccggcaga tgccgcggag ctttttgaca tcgagaatcg tgaccgtgcc gcgggagagt
    38941 gcgacgatgc cgtctttctc gaacgacttt aaggtgcggg aaacgacctc tctggcactg
    39001 ccgaggttcc gcgcgatctg ctcgtgcgtg gtggagagca ccggggaacc ggtgcggatg
    39061 cgttcatcct cgagatatcc ggcaaggcgc tggtcaagcc gcgtaaagag aagctggccc
    39121 atcgcccaca tgacttcgga aaaatgctcc gttgtttccc ggtaaatgta gttttcaacg
    39181 taaatattct gattcaacag tctgcggaac gtgctggccg gagtgatgag aactttcgtc
    39241 tcctcctcgg cctcaatcag aatatcaaag ctgatttcct gcatcgcaca ggaggcagac
    39301 agcgtgcaga cttcgccggg accggtggta aacagggtaa tttcccgctg ctcgccggac
    39361 tggataaaga tgcggagctt gccggagagg acaagcagaa cgccgagaca gtcatcgtca
    39421 cagctgtaga gaaaatcgcc gcgctgatac gtcgtcagga cggaagagag cacgagtgtc
    39481 tgctgttcat cggcggtaag ctttgaataa aatggataat gctcctgaaa acaggattcc
    39541 agaaaagatg acataaataa aactcctttc ggaagaagaa cgttcttttg taaactacgg
    39601 cagcagccgt ttcttccatg atagaaaaga atagaaagaa atgcaagaaa aaacgtatcg
    39661 gtttttgtgc cgatacgttt tttctgcatt tccgatatta cgctgtttat tttacagagt
    39721 taatcagttc ttccagctcc tcatagga
//
LOCUS       DAOMUF010000004        37267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_102_length_37267_cov_3.612007, whole genome shotgun sequence.
ACCESSION   DAOMUF010000004 DAOMUF010000000
VERSION     DAOMUF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 37267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37267
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102_length_37267_cov_3.612007"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..170
                     /locus_tag="PLN77_00655"
     CDS             24..170
                     /locus_tag="PLN77_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRM42820.1"
                     /translation="MTCYFVKPGDTLWDIAKKFYTTTDKIRELNELKTETLTPMQQLL
                     VERV"
     gene            500..1849
                     /locus_tag="PLN77_00660"
     CDS             500..1849
                     /locus_tag="PLN77_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRM42821.1"
                     /translation="MNMTSRKEYSYTTGALGNAVKTDLFTYRASGWKDQLISYNGSSI
                     SYDAVGNITAYQGRTLTWYRGSLLQSLTLNGKKAVYHYDSEGHRVQWKDLNGISHKNY
                     WCGNKLMGTKYGDVLVQFVYGADKSPLMLKKGEKEYYYLYNSQNDVIGLIDSDGKQVV
                     NYSYDAWGKQTGLTDISGENIGKLNPFRYRAYCYDDDTKLYVTASRYYDPELCRFLCA
                     DNFDAVKAKMFSMNGKNLYVYCCNNPVNAVDDDGDFGILMLAFPGIDPRKVAWNILKD
                     VFVYAVQCGMANEKVTLEGIAEVAIESAISSVLGDSQGIIFSVVVSGLKGGYLEYQES
                     GDMARAMGAGFYDAIMTLLEPKTYNAYVKNETIENALGAFTDFFQSPLLSYPKSGVLD
                     GVFGKPKKQANKNGSTLKKNSNINFSTKKVGGKKKNWQSNKPAISRKMQNIGLPALR"
     gene            1931..2416
                     /locus_tag="PLN77_00665"
     CDS             1931..2416
                     /locus_tag="PLN77_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42822.1"
                     /translation="MKVVWGKFGTGVDLIVCAISSYWWVSLWVNEKDAFHAYSLKTSI
                     IVFLGIMFFLSIAAYGTVTFLLKRIYIYEWGIMLSFWGYEKKIEWEDFTVIQRYNDHF
                     FFNISKQKDDSLFWCTYHPFTSIMIFDKFRGPGWGIIPLSADEFCELLTSYGVKYTES
                     K"
     gene            2512..2931
                     /locus_tag="PLN77_00670"
     CDS             2512..2931
                     /locus_tag="PLN77_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRM42823.1"
                     /translation="MKSIYRTILAVAGSFLAAQFGGWDAALEMLVCFMAIDWITGGVL
                     LPVVFKKSPKSENGTLESRAGWKGLCRKGMMLFFVLIAEKLDQLTQTNYLRDAVCIGF
                     ILNEAVSILENAGLMGVKIPEVLRSRIDVLRKEEQSK"
     gene            2928..3647
                     /locus_tag="PLN77_00675"
     CDS             2928..3647
                     /locus_tag="PLN77_00675"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM42824.1"
                     /translation="MRVDRSYCSNQNTWSENHPQCIVVHNTDNFAAGADARAHARAQY
                     QGNFQIMSAHYYVDDGEIAYQAAPHSRGCWHVGRNYGSKNLFGRYGNRNSIAVEMCVQ
                     KGYDYETAFLHTVELVKNLMNETGIPSDAVYRHYDICSKNCPSQIQKRGDWERFQRLI
                     RETEDGSEKSEYNPGIYRVTDPALNIRTGPGVEYPVVGVIKDQGSYTITEIRNKSWGR
                     LLSGAGWINCHKAYCFYGGRV"
     gene            4188..4670
                     /locus_tag="PLN77_00680"
     CDS             4188..4670
                     /locus_tag="PLN77_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42825.1"
                     /translation="MKAGYRKFYKYFSILVFMGCGYLMLNFWEEGKGQISSYNMQDKA
                     LMILWFMIYTFGTAAGIINTLTSIYIYEWGMQISFLKYKKKIEWEEFSVIRRYHNHFF
                     FNMSKQKDAGPYWAMFHPFTSFVIFDNSRNRSVHTMPLNADEFCELLTSYGVQYTERK
                     "
     gene            4696..5181
                     /locus_tag="PLN77_00685"
     CDS             4696..5181
                     /locus_tag="PLN77_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42826.1"
                     /translation="MRAGYRKFFMFAQVATLIGIGYLMTRFWEDAKKQCELYNLPQKL
                     WVIFGIMFICGIVSWGSVDALTRIYIHKWGMELAFLGYKKKIVWEDFSVIQRYKNHFF
                     FNVSKQKDSGLFFSTYHPFTSFVIFDKSEFSCVRTMPISADEFCDLLTSYGVKYKENK
                     A"
     gene            5429..5830
                     /locus_tag="PLN77_00690"
     CDS             5429..5830
                     /locus_tag="PLN77_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42827.1"
                     /translation="MEAMILVVFSFLDYLVCFISVAEMDCLKDRRYAAAKESGEFYKV
                     SEIESSLFNYVPRTYTIYYRRGSRRLKFGVRTRTTGNPLEMGGREYFIGPTGKREQIV
                     KDLDTLMKEVKKYARFDKVLVQTINGEPVKR"
     gene            5947..7296
                     /locus_tag="PLN77_00695"
     CDS             5947..7296
                     /locus_tag="PLN77_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM42828.1"
                     /translation="MNYLKERDGDLFSNADLKQLIVPLLIEQLLAIAVGMADSIMVSS
                     VGEAAVSSVSLIDTIFILLINMFTAIATGGAVVCGQYIGKKRPHKACEAADQLLLVTT
                     ALSVVIMAAVYLLKQFILHVVFGNITAEVERNCNIYLMIVAASIPFLAVYNGCAAIFR
                     AQGDSKTPMKISIVMNLINISGNALLVYALKFGVDGVAIPTLLSRMYAAAAAFVLVHN
                     KRYPVRCSYPVKVKPDFHMLKKILGIGIPNGLENSMFQLGKIMVLSMITQFGTASIAA
                     NAVSNTIAVFQTLPGMAIGNAVLTVSAQCAGAGDYEQVKYYAKKLHIIIYGLMIVCCL
                     GVIAVLPAVLHVYSLSAEATAMTEDIIVYHGICCMIIWPIAFNLPNVLRASNDVAFCM
                     ILSILSMWICRIGMSYVLGVRMGFGVFGVWVAMTMDWVVRAIFFVIRYRSGKWQNQM"
     gene            7363..8403
                     /locus_tag="PLN77_00700"
     CDS             7363..8403
                     /locus_tag="PLN77_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HRM42829.1"
                     /translation="MKNAKKLIPGAAIALVIAAAAKFLESLEESAGLHFIGASVIAMF
                     IGMIVNAFYKPNSVTAPGIKFTSKKILKFAIILLGASLNIRTVLTVGRFSLTVMVFTL
                     ATCFGLGALIGKALGLNWKTSSLINAGTGICGGSAIAAIAPVIEATDMDIAYGLSATF
                     LFDTVMIVVFPILGHWMGLSDAAFGLWAGTAVNDTSSVVATGYAFSEAAGDFATMVKL
                     TRTLAIIPAVLAFAAINVHLKRKAQAAEGTAVKVSIKSIFPWFILGFLAMSALTSLGL
                     IPAGTAAALKTISKFLMVAALAAIGLNTDFKSLCRSGAKPMLHGFIISLLVVLVAIAV
                     EFAIGIAPHGVL"
     gene            complement(8459..9274)
                     /locus_tag="PLN77_00705"
     CDS             complement(8459..9274)
                     /locus_tag="PLN77_00705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM42830.1"
                     /translation="MIKLIATDVDGTLVKDGTLLIDPEYMTVVSRLVDQGIYFVVCSG
                     RQFSSEQKLFAPIKDRLLYISDGGTVVRTPEKILKTYPMETALWKSMCRMVHDELPSC
                     DCFAATPDYCLAEDAGSRMFHWLRDSYGFDVREVPDLSKVENEDIIKFTIYHPTSCEE
                     LCSPNFIPAWNHQAKLAVAGKEWLDCNARGVSKWTGLSFLMNYFGLTSDEVCCFGDNL
                     NDIEMLQNAGESYAVSNARAEVIASAKHTCPPYWENGVLQVLKTFLKETTNPF"
     gene            9606..10436
                     /locus_tag="PLN77_00710"
     CDS             9606..10436
                     /locus_tag="PLN77_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRM42831.1"
                     /translation="MAFIQVSFMSKTLMRTVPLQVILPVDKFTFGQPEREEKPFKTLY
                     LLHGIFGNETDWVHGTRIQRWAEEKNLAVVMPAGENAFYVDQPSIGAMHGQFIGEELV
                     EITRKMFPLSRKREDTFIGGLSMGGFGALRNGLKYHDTFGAVICLSGALHVLENPEES
                     RADSFAHEEGYFGNLVEAAKSDKNPAVLIEQLKEARKKDPSVNVPTVFQACGTEDGLL
                     EVNRLYHKRMEESGMDIVYYESKGGHEWDFWDEWIKKALDWLPLEEKTEGLNSGNVNV
                     "
     gene            10457..10729
                     /locus_tag="PLN77_00715"
     CDS             10457..10729
                     /locus_tag="PLN77_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42832.1"
                     /translation="MPETRNDELYRALKHNGYPDDFCREIAYRQMNTDFTATRMLGYL
                     YRVTSPRAEDVVDEMLAILSDRKAWIEKKQSEEAQAAISRMYREGL"
     misc_binding    10809..11111
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11292..12995
                     /locus_tag="PLN77_00720"
     CDS             11292..12995
                     /locus_tag="PLN77_00720"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRM42833.1"
                     /translation="MKLEKLVGERFKERPADCVIDSHAIMVKGGYIKYMANGIYSSYL
                     PLRRIVRKIEQILREEMDKIDGQEVQFPVVMPASLWDESGRYDSIGDELLRFTDRNNA
                     KMVLGMTHEEAAVHLVREYAQSYTKYPFMIYQIQTKFRDEARPRAGLIRVREFTMKDA
                     YSFHTSQEDLEQYYEKCHAAYERIFERVGVPEVVSVKSDSGMMGGNISHEFMLLTPVG
                     EDSIVLCDSCDYRANMEAAENISDIARDAESAALEKVYTPNVHTIEDVCNFFGDETKN
                     SCKAVVYQQNVDDKYVVLFIRGDLEVNETKLVNFLGEQVHAAVITEECGLNAGYIGPV
                     NLKVNGDAVVLYDKSLEGRNNLSCGANEAEHHYKGLDMERDVPNAEYHDFAKIQEGGI
                     CPKCGKKTVKISRGIEVGNIFQLGTKYTKSMNMTYVDANGESKTPIMGCYGIGVGRLA
                     ASVCEAHHDEYGPIWPKAIAPWQVHLCAVRVDDEEVRAYADKLYEDLQNAGIEVIYDD
                     RSVRAGVMFADADLLGIPLRIIVSPKNMKQGVVEVASRDKTLKTQIPLENVMEEIKQY
                     L"
     gene            13070..13573
                     /locus_tag="PLN77_00725"
     CDS             13070..13573
                     /locus_tag="PLN77_00725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM42834.1"
                     /translation="MELWDIYDENKQPTGRTMKRNDWHMKPGEFHLTVLGVIARPDGT
                     FLITKRVMTKAWAPGWWEVSGGAAQAGESSWEAVRREVREETGLDVSKADGGYLFTYR
                     RINPDEGDNYFVDVYRFVMDVKDEDLHLQEEETDGYRFATLDEIKGYAAEGKFLHYDS
                     IRQAFEL"
     gene            complement(13698..14996)
                     /locus_tag="PLN77_00730"
     CDS             complement(13698..14996)
                     /locus_tag="PLN77_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRM42835.1"
                     /translation="MVRNRKETTEKSKKKPGLSTQIFIALLAGALLGVVIHYWIPSSY
                     IKDTVIVEGVLYVIGQGFIRLMQMLVVPLVFCSLVCGSMSIGDSKTLGKVGVKTIGFY
                     LVTTALAVCIALGTALLIHPGLGLNMSAVQKADLSSTAADSSVSLVDTLLNIIPKNPI
                     GSMANGDMLPIIVFALFVGVMLAKLGNRASVVSNFISQFNDVMMEMTMTVMKVAPVGV
                     FCLIARTFATVGFSAFAPMLKYMGNVTLALALQCFVIYQVLLFVFTRLNPIKFVKKFL
                     PVMGFAFSTATSNATIPMSIDTLNKKMGVSKQISSFTIPLGATINMDGTSIMQGVAVI
                     FIAEAYGIHLTPANLATVVVTATLASIGTAGIPSVGLVTLAMVLNSVGLPTEGIALIM
                     GIDRILDMLRTAVNITGDAVCTTIVSHQEKALNREVFNRN"
     gene            complement(14996..15841)
                     /gene="rfbD"
                     /locus_tag="PLN77_00735"
     CDS             complement(14996..15841)
                     /gene="rfbD"
                     /locus_tag="PLN77_00735"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRM42836.1"
                     /translation="MKKIWITGAEGHIGTALLELLEGVEYQLLPTDIHEVDITKIDEV
                     TQFVYVNRPDVIINCAGMTDVAECEKNIDEAYRVNAIGVRNVALAANDIDAKVIQIST
                     DDVFDKESRIPYNEFDTVHPRTIYGKSKEAGEKILTQLLNRFVIIRSSWIYGIGRDFV
                     DEVLRNAAKGGTMEVPNNQYAAPTSAFELARVICHFIDNEEYGLYHVVCPGSCSRYEF
                     ARTILEYTGKTGDLDLYPVVARDDVRPTYSVLDNMMLRLTGIPEPPDWKTALKEYLEE
                     TGGIE"
     gene            16054..17385
                     /locus_tag="PLN77_00740"
     CDS             16054..17385
                     /locus_tag="PLN77_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM42837.1"
                     /translation="MCNGTIMDKLISFSLPLMVSGILQLMFNAVDIIVVGRFSGSQAL
                     AAVGSTTALINVFINLFMGISLGANVLAARYYAAGRDREMSETVHTSIMLALISGVVM
                     AFVGVFFAKGALELMDTPADVIDQSALYMRIYFMGMPFFMLYNYGAAILRAVGDTKRP
                     LYFLIVSGVINAGLNMFLVIVFSLDVAGVAIATVVSQAISCVLVLRCLYKSEGSYQLR
                     FSKLCLKKDYIIPIFQVGIPAGIQSTVINFSNVLLQSSVNSFGSIAMAGYTAANNVLG
                     FLYTSVNSVTQACMSFTSQNYGVGKYKRMDKVLIDCLILSSGMALVMGGGAYLFGTEI
                     LQIYTGDPEVIRCGMEILSITTVPYFLCGIMDLFPGALRGMGCSAVPMILSVIGTVGT
                     RIVWIFGIFPQHRSLYVLFISYPGSWIITIIMQVICYLIMRKKVHSRAAAV"
     gene            17548..18774
                     /locus_tag="PLN77_00745"
     CDS             17548..18774
                     /locus_tag="PLN77_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRM42838.1"
                     /translation="MKKFYAWYQKHITGAIFTGLILGILTGLLLADRFTPILTVTGVI
                     GGIYMNALNMMIFPMVFCSIVMGICSIGNAKTTGKITGYSMIFFLCTTAIASAVGIII
                     PRLIRLGKGVHFEMATSDIQATEMTSILDTIKNLIPSNPVKAFAEGNMLQVLVFAVVV
                     GFTLIAVGEKGQPLLNVIDSLNEVCLKVISTVMYFTPIGVFCTIVPVVEANGTKTIVS
                     LATQLIILYVAFYGFAIVVYGGAVKLIGKTSPVKFFKAIMPAALNAFGTCSSSATIPI
                     SKRCVEKELGVSNQISSIAIPLGATVNMDAVSILMSFMIMFFANACGINVSISMMVII
                     LLANVLLSVGTPGIPGGAIASFAALATMAGLPAGVMGVYISINTLCDMGATCVNVIGD
                     MAGCVVLQEKIKLEEN"
     gene            complement(18842..20056)
                     /locus_tag="PLN77_00750"
     CDS             complement(18842..20056)
                     /locus_tag="PLN77_00750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM42839.1"
                     /translation="MIQTKDSNLTFRPLTIDDAEQYNALLRYAFQVTEQELSESGWKD
                     DEIKQSKFPVLVRADVLGCFCEEDLVSQFAVYPLDMNIYGVRYPVGFVTSVCTYPEYT
                     GQGIMKKLMIRSLTRMRENHRSFALLYPYSIPLYRNLGWEIISNKMTYTIKDTQVPRK
                     LKAPGYVRRVAWDDKDFKELHTKFASKTHGCLYRNNLAWEEYFRWDEDDTVVAIYYSA
                     DDVPYGYMVYLISSDIMHIKEMIYLNREAQLGLWEYIHAHDSMIDEVKGNNYYSEPIA
                     FELEDSDIKETIRPYSMGRIIDVVQFMEHYACDPDEPDVCIRFEIEDELLPWNNDSFT
                     FFFEKGHCVPTDREPDHVMKMTIASLTTLLLGYKTASKLYEMARIETTPQTVECLDDL
                     LFHHIPYVSDYI"
     gene            20325..21020
                     /locus_tag="PLN77_00755"
     CDS             20325..21020
                     /locus_tag="PLN77_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRM42840.1"
                     /translation="MFNPETRKYVWDVCKKNYYDYGINAFWLDNSEPDYGVYDFDHYR
                     YIEGPALSCSNIYPQLYSRVFYDNMKDLGDVPSTFEAFYDQLQAGLNMGLAGIPWWTT
                     DIGGFMTDDVNDPDFQQLLIRWYEFAVYSAVLRMHGDRGPYNIPMLDDRDFGGGCLHT
                     GQPNELWSYGEENYKIMKKYYDIRIEMHDYIKKLYDEASENVQGTEKSMKAARRSTPM
                     HRSIRFRYLNENS"
     gene            21304..23301
                     /locus_tag="PLN77_00760"
     CDS             21304..23301
                     /locus_tag="PLN77_00760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM42841.1"
                     /translation="MTQKQAERLVEHLKNIFMYVRVLRPDEIGDFIIDEENGKLCECY
                     TVWNKAMPCLNCISEKALREKSQKSKLECVASNVYQVIARYVEIEGEPCVIEMINRLD
                     DETLMDSEGRQNLVSKLNSYSEELYRDALTGVFNRRYFEDQIRDASFCCGVAMIDLDD
                     FKLYNDTYGHNAGDMALDTIVKTVNRCTRRTDRLIRLGGDEFLLVLPEMDEENFVQKL
                     KQIQSQIHEAQVPGYSKMRLSVSVGGVLTRDETIGEAAMRADQLMYLAKQRKNMVVTE
                     KDQILGCQDDVVRQENRREKQRILIVDDSEMNRIILSEILQDEYDIIEASTGEECLRL
                     LEEYGTGLALILLDIVMPGMDGFGVLDYMNRNHWIEEVPVIMISSEDSTTFVRRAYEQ
                     GVSDYISRPFDAKVVYQRVFNTIKLYAKQRRLVTLVTEQIYEKEKNNQMMISILSHIM
                     EFRNGESGLHVHHINVLTSMLLERLVQKTDRYHLSWSDQLMITTASALHDIGKMGIDE
                     NILNKPGRLTKEEFEEMKKHTLIGASMLKSLELYQDEKLVQIAYQICRWHHERYDGKG
                     YPDGLKGEEIPIAAQVVSIADVYDALTSERVYKSAYTHEKAIEMILNGECGTFSPLML
                     ECLLDIKDAICEELNTISEEKQTSIQIEKLKKNLKSGGGQS"
     gene            23571..24362
                     /locus_tag="PLN77_00765"
     CDS             23571..24362
                     /locus_tag="PLN77_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRM42842.1"
                     /translation="MNQLEVLRESLGQCDEIILDALLMRNRIVEDIMVYKEANNVPIL
                     QPEQEAKQKEWLERRMQEKRHKNEVADVFESITRNSKKIQARKLFNYNIVLIGFMGAG
                     KSTISDYLKTVFAMDIIEMDQIIAERAGMSISDIFETYGEQYFRDCETNLLIEMQSRT
                     NVVISCGGGTPMRECNVVEMKKNGRVVLLTAKPETILDRVKDSHDRPLIENNKTVPFI
                     ADLMSKRREKYEAAADIIIETDGKSELEICEELIHSLRQMDAKAE"
     gene            24667..26418
                     /locus_tag="PLN77_00770"
     CDS             24667..26418
                     /locus_tag="PLN77_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM42843.1"
                     /translation="MRKRNKVVSLLLAGCMAAGLLAGCGSSGETQVATADNATAASTS
                     TGAADSAAPETAGEVDGQINLRAVAFGNNFDVQDMGWRWMMGECYEGLMRDVADENGD
                     RFEYAGAESMDVSDDGLVYTFHLRKDAKWSDGQPVTAADYEYGWKRLLNPEYGYSYSF
                     YLFNVVGAEEYYNGQGSADEIGIKAVDDYTFEVTLKVADPTFQSKLVATPLYPTRQDV
                     AEAAGDQWGKDWTKCVYNGPFAMTNLVEDNSMTWVKNDQYWDKDNVKLNQVNWYCVAE
                     NATAATMFDNGQLDVFDAAGDYIAKYDKEVEAGNMQTMTTEYPGTMVLCYEQSEGGKS
                     GLMKNVNIRKAISYSINREEMVSAVYGRYTAAYGFVSPAITLDGTSYRKQASETMKEE
                     YEQYAGDADKLKALFQKGLDELGITDKPEDITITLLSQGSATENQLEREYLQQSISQN
                     LGVKVELNTVGDYQMFANERDNKNYDIFVGGWFSDYNDPLDFLNVMKTGVYDSYGLYS
                     NSEYDSLIDSLTGENDNAKRLEIYQKLEDLLVAQDCGVAPLYYSDKHYYYQNWVKDFY
                     TSSFGASQELYRASVQK"
     gene            26533..27489
                     /locus_tag="PLN77_00775"
     CDS             26533..27489
                     /locus_tag="PLN77_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM42844.1"
                     /translation="MPKYLLRRFAEMLITLFLIATATFFLLEAVPGDPLTERASKLSP
                     TVKENLYKRYGLDKPMMERYVITMKGICKGEFGESVVYAGQTVQKLIHDRLPVSARLG
                     IQQVLVGITIGLLLGILAAMRRGSFWDYMVIALSVLLISIPNLIFGLMLQKIFAGNLK
                     VLPTIGWPAGKDLWFGGWEYTILPTLTGCFSYIATYARLLKTSMLDVINQDYVLTAES
                     KGLSRGQIIRRHILRNSFIPVITQLPMSVAMCITGSFFIESIFSIPGIGQYYVTAVNN
                     QDLSIIMGETVIIAALYIGVLFITDLLYVVVDPRISLTGKSR"
     gene            27500..28417
                     /locus_tag="PLN77_00780"
     CDS             27500..28417
                     /locus_tag="PLN77_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM42845.1"
                     /translation="MAALTPEKFKVIGYTEEDANRIDRPTISYWQDAWRRLRKNPIAM
                     GSLVVLGILLVMVIIGPHIKGYDYVSMNIAEKNQGVSSKYWFGTDNLGRDLFSRVWVG
                     ARASLIIAIVATAIKLIIGTVYGALMAHFGGWVDEVLMRIIEVINSIPSLLLTILIMM
                     ILGNNLFALLVALSVTAWCNTARQVRGMIKQLRETEYVYAAEVLGAKPLRIILKHYVP
                     NMLGILILDTATAIPMFIFTEAGLSFLGIGLKAPSISLGVLISMGQQNMDFYPSQLLF
                     PCLVLCIIVMAFNLLGDGLRDALDPRLRQ"
     gene            28436..29467
                     /locus_tag="PLN77_00785"
     CDS             28436..29467
                     /locus_tag="PLN77_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM42846.1"
                     /translation="MNEQNNPILEVKNLRVSYHTYAGEVKAVRGVQFSLEKGQALAIV
                     GESGCGKSVTAKSIMGLIKAPQGEIKENSEILYHGENILKYNKKQWQHYKGGECAIIF
                     QDALASLNPTMRVGKQIMENLMGHKNMTKQEAAKEAVEMLRMVGIPEPEKRVRQYPHE
                     FSGGMRQRVMIAIAFACDPKILICDEPTTALDVTIQGQILDIIKNLQKKNQTSVIMIT
                     HDLGVVANIAQKIAVMYSGIIVESGTCEDIFYRPRHPYTWALIRSVPRLDLKNKQELA
                     TIPGTPPDLLNPPVGCPFCTRCSYCMPICKEEMPEATEFGGEHKAACWLHHPMAPKVE
                     MPNLEGGEL"
     gene            29467..30468
                     /locus_tag="PLN77_00790"
     CDS             29467..30468
                     /locus_tag="PLN77_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM42847.1"
                     /translation="MENNEKEVLFSVSHLKKYFQVGNHATLKAVDDVSFEIYKGETLG
                     MVGESGCGKTTCGRTCIGLYDKTDGQVLYKGTDVHALNGKERFAFKKQVQMVFQDPYG
                     SLDPRMTVADIIGEGIDIHHLAKNKKERQEMIYKYLELVGLNREHANRFVHEFSGGQR
                     QRIGIARALAVQPEFIVLDEPISALDVSIQAQIVNLLVDLQKKMGLTYLFVAHDLSMV
                     KHISDRVAVLYLGTLVELTTSEELYANPRHPYTQALLSAIPIPDPKVEAERDRKKIRL
                     EGEVPSPINTKPGCKFQGRCKYAKDICRQQMPELKDVGNNHFVACHFCDALEENKEL"
     gene            30472..30825
                     /locus_tag="PLN77_00795"
     CDS             30472..30825
                     /locus_tag="PLN77_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42848.1"
                     /translation="MEKIKNICRDLGKAFTIGLLSGGAAAAVLLLGGFLFGGFTFRAA
                     LEAAKDGLLLIAALGMFVIAGMLMVKGKNPEKFTQKEKWRVHFAVLGYKSVFAVLCVS
                     WVLWAAAADYLLYRM"
     gene            complement(30907..31494)
                     /locus_tag="PLN77_00800"
     CDS             complement(30907..31494)
                     /locus_tag="PLN77_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM42849.1"
                     /translation="MKKMILVTSPPACGKTYISKQLAKELKHVVYLDKDTVIPLSNRV
                     FKAGGQEINRSSDFFEENIRDYEYEVTVNFGLEALEYDDIVLINAPFTREVHDEKFLK
                     DFSKKLADHGAKLVVIWVVTKPEICHQRMIDRNSVRDTWKLEHWDEYVKTIDYSIPEA
                     LKKMSSCLDLMLFYNSSDEEYQKSMEMVISRLEDE"
     gene            31662..32717
                     /gene="ispG"
                     /locus_tag="PLN77_00805"
                     /gene_synonym="gcpE"
     CDS             31662..32717
                     /gene="ispG"
                     /locus_tag="PLN77_00805"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRM42850.1"
                     /translation="MSRANTRVVKIGDRVIGGGNPILIQSMTNTKTEDVEATVKQIQA
                     LTEAGCDIIRCAVPTMEAAKALSEIKKQISIPLVADIHFDYRLAIAAIENGADKIRIN
                     PGNIGSRDRIQAVVDAAKERQIPIRVGVNSGSLEKELVEKYNGVTAEGLVESALDKVR
                     IIEEMGYDNLVISIKSSDVMMCAKAHELITNQTDHPLHVGITEAGTLYSGNIKSAVGL
                     GIILYQGIGDTIRVSLTGDPLEEIKSAKRILKTLGLRKGGIEVVSCPTCGRTQIDLIG
                     LANQVETLVQGYDLDIKVAVMGCVVNGPGEAKEADLGVAGGKGVGILIKKGEIVKKVP
                     ESELLSVLKDELDHWGK"
     gene            32738..>37267
                     /locus_tag="PLN77_00810"
     CDS             32738..>37267
                     /locus_tag="PLN77_00810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HRM42851.1"
                     /translation="MEKQFQEVFPDLRIEGGMAELLDTVEVSRVSINKKKDLLRIYVV
                     SHQWIHKKYIYQLERQIEEQLFSNVPLRVKIIERFYLSRQYTPENFLEVYRPSILLEL
                     KNHSVFLNHLFSTAEITFPETNKMHLVLVDSVVAKEREEELVHILEKIFCERCGFDLI
                     VETEMRKPEGESRAMRNSELRIREEVRHVLAHTHYGEMLGEKEQNRMEEQAEAAGEQP
                     KASAAAKDGEAKKEKAADGGRGKGFSGGGRGSFKKGKGSFGRNADNQPRKSSNPDVLY
                     GRDFEDESVEILKIGDEIGDVVIRGRVQSVDMREIRNERTIFMFTITDFTDTIGVKIF
                     VQNAEVPELKDAIKKGAFIKVKGKTTVDAFDHDLTVMSVWGIKKITDFRTGRQDTSPV
                     KRVELHCHTKMSDMDGVTDAARLVQRAYEWGHPAIAITDHGVVQSFPEANHAIEAIDG
                     AYRKKYQAEHPDATKDELKKVSAPFKVIYGMEAYLVDDLKDIVVNSKGQDIHGSYVVF
                     DIETTGFSPVVNKIIEIGAVRVENGAIVDKFSTFVNPKVPIPFRIENLTGINDNMVLD
                     APDIETVLPKFLEFSEGAVMVAHNASFDMSFIEHNCVLQGIEREFTTADTVAMARFLL
                     PGLNRFKLDTVAKAVGVSLENHHRAVDDAGCTAEIFVKFVKMLEERNILTLDDLNAQG
                     KVSEEAVRKLPSYHAIILAKNETGRVNLYRLVSESHLKYYNRRPKLPKSVYLKYQDGL
                     MIGSACEAGELYQAILRGEPDAAIARLVDFYDYLEIQPIGNNAFMIKKEDVSAVESEE
                     DLKEINRRIVKLGEQFHKPVVATCDVHFMDPQDEIYRRIIMTGKGFDDADEQAPLFLR
                     TTEEMLEEFSYLGSEKAEEVVITNPRKISDLVEKISPIRAGKFPPVIEDSDKTLRRIC
                     YDRAHEIYGEELPEIVSARLERELNSIISNGYAVMYIIAQKLVWKSNEDGYLVGSRGS
                     VGSSFVATMAGITEVNPLSPHYYCESCHYSEFDSPRVKEYVGRAGCDMPDAVCPNCGK
                     PLKKAGFDIPFETFLGFKGNKEPDIDLNFSGDYQSKAHKYTEVIFGAGQTFRAGTIGT
                     LADKTAFGYVKNYYEERGQHKRNCEIDRIVEGCTGIRRTTGQHPGGIIVLPFGEDINS
                     FTPVQHPANDMTTDIITTHFDYHSIDANLLKLDILGHDDPTMIRMLEDITHLDAQTIP
                     LDNPEVMSLFKSTEALGIKPEDIGGCPLGCLGVPEFGTDFVIQMLLDTKPQSFSDLIR
                     ISGLSHGTDVWLGNAQTLIEEGKATISTAICTRDDIMIYLIDKGLESELSFTIMESVR
                     KGKGLKPEWEEEMKAHDVPDWYIWSCKKIKYMFPKAHAAAYVMMAYRIAYYKIFYPLA
                     YYAAYFSIRASAFSYELMCMGRDRLEYYMKDYEKRKDTLTQKEQATVKDMKIVQEMYA
                     RGFDFVPVDLYKAQAHRFQVYDDKHLMPALDSIEGLGDKAADAVVLAARNGKFLSKDD
                     FRDRTKVSKTIIDFMA"
BASE COUNT        10247 a         8325 c         9942 g         8753 t
ORIGIN      
        1 aagaactgca gagcctcccg ggcatcacct gctacttcgt caagccgggg gatactctgt
       61 gggatatcgc gaagaaattc tatacgacga cagataagat cagggaatta aatgaactga
      121 aaaccgaaac actcacaccg atgcagcagt tattagtgga gcgggtatag cggaaaagac
      181 agcctgctgc cttaagaatg gagttaatcc atcaggcagc aggctgtctt tttgcgagtt
      241 ccatgccgat ctgcattgca gaaagaacag gtacactttt gtaccgggaa gaaaaacggg
      301 aattacaacc ttgcttctca aagaaatcaa caataacgga aagaaactgt cctttgttta
      361 tgatgctgtg ggaaatattg ttacgatttc cgagaatggt gttcagaaag taaaatatac
      421 gtataatgct ttgagtgaac tgacccgtgt ggattctgca tgggaaaata agagtattac
      481 gtatacctac gatgccggaa tgaatatgac gtccagaaaa gagtattcct atacaacggg
      541 agcactgggc aatgcggtaa agacagatct gtttacctac cgtgcttcag gatggaaaga
      601 tcagcttatt tcctataatg gcagcagcat ttcctatgat gcggtcggca acattacagc
      661 ttatcaggga agaacactga cctggtacag aggaagcctg ctgcagagtc tgaccttaaa
      721 cggaaagaaa gcagtttacc attacgacag cgaaggacac agagtccagt ggaaagacct
      781 gaacggaatc agccataaga actactggtg cggaaacaaa ctgatgggaa cgaagtatgg
      841 agatgtactg gttcagtttg tttacggtgc ggataaatcc ccgctgatgc tgaaaaaggg
      901 agaaaaagag tattattact tgtacaacag ccagaatgat gtcataggac tgattgacag
      961 cgatggaaaa caggtagtaa actattctta cgatgcatgg ggaaaacaga ctggtctgac
     1021 cgatatcagc ggagaaaata tcggaaaact aaacccattc cgttatcgtg cttactgcta
     1081 cgatgacgat accaaactct atgtcaccgc cagccgttat tatgatccgg aattgtgcag
     1141 attcctttgt gcggacaact ttgatgctgt aaaagcgaaa atgtttagca tgaatggaaa
     1201 gaacctttat gtgtactgtt gcaacaatcc ggtgaatgcg gtagatgatg atggtgattt
     1261 tggaatttta atgctggcat tcccaggaat cgatcccaga aaagtagcat ggaatatctt
     1321 aaaagatgta tttgtatatg cggtacagtg cggaatggcg aatgagaaag taaccttaga
     1381 aggaattgca gaggtggcca tagaaagtgc aatcagtagt gttttgggag atagccaagg
     1441 aattatcttt tcagtagtag taagtggttt gaaaggagga tatttagagt atcaggaatc
     1501 aggagatatg gcaagagcaa tgggggcagg attttacgat gctataatga ccttgttaga
     1561 acctaaaacg tataacgcat atgtaaaaaa tgaaactatt gagaatgcat tgggtgcttt
     1621 tacagacttt tttcagtctc cattactgag ctatcccaaa agcggtgttt tggatggagt
     1681 gtttggaaaa cccaagaaac aggcaaataa aaatggatca acacttaaga aaaacagcaa
     1741 catcaacttt agtacaaaaa aagttggggg gaaaaagaag aactggcaga gtaataagcc
     1801 tgcaatttcc agaaaaatgc aaaatatagg acttccagcg cttagataga aaagttttaa
     1861 gatgcctttg cgcgaagaaa aatagttttg tacaaaggca aagcgtaaga aataacgagg
     1921 agaagattgt atgaaagtag tatggggaaa atttggtacc ggcgtagacc tgattgtgtg
     1981 tgctattagt agttattggt gggtgagcct gtgggtaaat gaaaaagatg cgttccacgc
     2041 ctattcatta aaaacaagta tcatagtgtt tctaggaata atgttctttt taagcatagc
     2101 agcttatgga actgtgacat ttctactaaa acgaatttat atttatgaat ggggaataat
     2161 gttatctttt tggggctatg aaaaaaagat agaatgggaa gattttactg tgatacaaag
     2221 atacaatgat cattttttct tcaacatatc gaaacagaaa gatgacagtt tgttctggtg
     2281 tacctatcac ccatttacca gtatcatgat attcgacaag ttccgcggac cgggctgggg
     2341 aatcataccg ctcagcgccg acgaattttg tgaactcctc acttcatacg gtgtcaagta
     2401 tacggaaagt aaataacata caaaaagagt ggaagtggcc gtaagatcca cgaattacca
     2461 gccacttccc tttttatatg caaatttaga aaaagaaaga aggaaatgcc aatgaaatca
     2521 atttatcgca cgatccttgc cgtagcaggc agcttccttg cggcacagtt tggaggctgg
     2581 gatgctgcgc tggaaatgct ggtctgcttc atggcaattg actggatcac aggaggtgtt
     2641 ctgcttcctg ttgtttttaa aaagagtccg aaatctgaaa acggaacgtt ggagagccgt
     2701 gcgggatgga aagggctttg cagaaaaggg atgatgctgt tctttgtgct gatcgcggaa
     2761 aaactggatc agctcacaca gacgaattac ctgcgggatg ccgtctgcat tggatttatt
     2821 ttaaatgaag cggtttcgat cttggaaaat gcagggctga tgggtgtgaa aatccctgag
     2881 gttctgcggt caaggattga tgtgctgaga aaggaggagc agagtaaatg agggttgaca
     2941 gaagctattg tagcaaccag aatacatggt cggaaaatca tccgcagtgt attgtggtac
     3001 acaatacaga caattttgcc gccggagccg atgcccgcgc gcatgcaaga gcgcagtacc
     3061 agggaaattt tcagattatg tcggcgcatt attacgtaga tgacggggag atcgcttatc
     3121 aggctgcgcc gcacagcaga ggatgctggc atgtcgggag aaattatgga agtaaaaatc
     3181 tattcggcag atatggaaac cggaacagca tcgcggttga gatgtgcgtg cagaaaggat
     3241 atgattacga aaccgcattt ttacatacgg tggagctggt gaaaaacctg atgaacgaga
     3301 cggggattcc atcagatgca gtttaccggc attacgacat ctgcagcaaa aactgcccgt
     3361 cccagattca gaagcgggga gactgggagc ggttccagcg tctgattcgg gaaacagaag
     3421 acggcagtga gaaaagtgaa tacaatcccg gcatctaccg tgtaacagac ccggcgctca
     3481 acatccgcac aggtcccgga gtagaatacc cggtggtggg agtcatcaaa gaccagggaa
     3541 gctatacgat cacagaaatc agaaacaaaa gctggggcag actgctgtcg ggagcgggat
     3601 ggatcaactg ccataaagca tattgttttt acgggggaag agtataaaat cccatctttg
     3661 caaaacgatg tcatcggacg gattgacagc gatggaaagc agggagtaaa ctattcttac
     3721 gatgcagggg gagaactggt aaataccatg gatacaagtg gtgcgaatat tgcacagtcg
     3781 aaagaaataa cgaggagaaa attgtatgaa agtaatatgg ggaaaatttg gtacaggtgt
     3841 agatttgatt ctgtgtgtta ttattaatta ctatgtggtg agtatatggg taaatggaaa
     3901 agatgagttc catgcatatt cattaaaaac aagtattata tttctaatag aggtaatgct
     3961 cgttttaaat atggggaaaa cagaccggtc tgaccgatac cagcggagaa aatatcggaa
     4021 agctcaatcc attccgctat cgtgcttact gctacgatga cgacaccaag ctctatgtca
     4081 ccgccagccg ctattacgat ctggaattgt gcagattcct ttgcgcagac aactttgatg
     4141 ttgtaaaagc ggaaatgttc aaaaagttaa attagtaggt ggcaaatatg aaggcaggat
     4201 atcgtaaatt ttataaatat tttagtatat tggtatttat gggctgcgga taccttatgc
     4261 tgaatttttg ggaagaggga aaaggtcaaa ttagttctta taacatgcaa gataaagcac
     4321 ttatgatatt gtggtttatg atttatactt ttggtacggc tgctggaatt ataaatactt
     4381 taaccagtat ttacatttat gaatggggaa tgcagatctc atttttgaaa tataagaaaa
     4441 aaatcgaatg ggaagaattt tctgttatac gaagatatca taatcatttt ttcttcaata
     4501 tgtcaaaaca aaaagatgca ggtccgtatt gggcaatgtt tcatcccttt accagttttg
     4561 taatattcga taattcgcga aatcgttctg ttcataccat gccgcttaac gccgacgaat
     4621 tttgtgaact gctcacttca tatggtgttc agtacacgga aagaaaataa agatcgaagg
     4681 aattaaagag aaaatatgag agcaggatat cgtaagtttt ttatgtttgc acaagttgct
     4741 acattaatag gcatcgggta tcttatgaca agattttggg aagatgcaaa aaaacaatgt
     4801 gaattatata atttgcctca aaagctttgg gtaatatttg gaataatgtt tatttgtggg
     4861 atagtgtcat ggggaagtgt agatgcttta acacgtattt acatccataa gtggggaatg
     4921 gaacttgcct ttttgggata taagaaaaaa attgtatggg aggatttttc cgttatacaa
     4981 agatataaaa atcatttttt ctttaacgta tcaaaacaaa aagatagtgg tctgtttttt
     5041 tcaacatatc atccatttac tagctttgtg atatttgata agtcagaatt tagttgtgtc
     5101 cgtactatgc caatcagtgc cgacgaattt tgtgatctcc tcacttcata cggtgtcaag
     5161 tataaggaaa ataaagcatg agaagagtgg aagtgatcgg atttctgttg tttttacggg
     5221 ggaaagagag tagtataata catgtataat tacagctttg gaggaaaaac agttgcttga
     5281 ctggatcaat gtttttaaag taaaccggaa aattaaatcc cgtctctggc cgtgaattaa
     5341 gattatttta agtattgttc ttttctttgt ggtattttac ggtgacatct atgttcatat
     5401 tctggatgga acaaatctgg gacgaattgt ggaagcaatg attctggttg tgttttcgtt
     5461 tctggactat ctggtctgct ttatttccgt agctgaaatg gactgcctga aggaccggcg
     5521 gtacgcggcg gcgaaagaaa gcggcgaatt ttataaggtt tctgaaatag aaagctctct
     5581 gtttaattac gtgccgcgta cctatacgat ttattaccgg aggggtagca gaagacttaa
     5641 attcggtgta cggacaagaa cgacaggaaa tccgctggaa atgggcggca gggaatattt
     5701 cataggaccg acaggaaagc gcgagcagat tgtgaaagac ctcgatacgc tgatgaaaga
     5761 agtaaaaaaa tacgcaagat ttgacaaagt cctggtacag acaatcaatg gagaaccggt
     5821 aaagagataa tgccggttct tcttgctttt acgtaaaaat gcaggtaaga taaaaacagc
     5881 ataagtgtaa tacacatgcg taaaaactca tcaacggaga aggtatgaaa atggaggaaa
     5941 caaaagatga attatctgaa ggaacgggat ggtgacctgt tctccaatgc ggatctgaaa
     6001 cagctgattg tgccgcttct gattgaacag cttcttgcca tcgcagtcgg catggcggat
     6061 tcaattatgg tatccagtgt cggcgaggcg gcggtgtcga gtgtgtcgct gattgataca
     6121 atctttattc tgctgatcaa catgttcacc gcgattgcaa ccggcggagc cgtcgtgtgc
     6181 ggccagtata tcggaaagaa gcggccacac aaagcatgtg aggcagctga ccagcttctg
     6241 ctggtgacaa cggcgctgtc agttgtgatc atggcggcgg tctatctttt aaagcagttt
     6301 attttgcatg ttgtgtttgg aaacattacg gcagaggtag aaagaaactg caatatttat
     6361 ctgatgatcg tagcggcctc cattccgttt ctggcggttt acaacggctg tgcggccatt
     6421 ttccgtgcgc agggtgattc gaaaacgccg atgaaaatat ccatcgtcat gaacctgatc
     6481 aatatttcgg gaaatgcgct tctggtctat gcgctgaaat tcggcgtgga cggcgttgcg
     6541 attccgacgc tgctttcacg tatgtacgcg gctgcggcag catttgttct cgtacataat
     6601 aagcggtatc cggtgcggtg ctcttatccg gtgaaagtaa aaccggattt ccatatgctg
     6661 aaaaaaatcc ttggcatcgg cattccgaac ggcctcgaga acagtatgtt ccagctcggc
     6721 aaaattatgg tcttaagtat gatcacacag ttcggaacag cgtcgatcgc ggcaaatgcc
     6781 gtgtccaaca cgattgctgt cttccagaca ctgcccggta tggcgatcgg aaatgcagtg
     6841 ctgaccgtaa gcgcacagtg cgcgggagcg ggagactatg agcaggtaaa atactatgcg
     6901 aaaaagctgc acatcatcat ttatggcctg atgatcgttt gctgtctcgg tgtcatcgcg
     6961 gtgcttccgg cagtgcttca cgtttacagt ctgtctgcgg aggcaacagc catgacggaa
     7021 gatatcatcg tttaccacgg catctgctgt atgataatct ggccaattgc cttcaatctg
     7081 ccgaatgtac tccgcgcgtc caatgatgtg gcattttgca tgattctctc gattctttcc
     7141 atgtggatct gcagaatcgg catgagctat gtgctcggtg tgcgtatggg attcggcgtc
     7201 ttcggtgtct gggtcgccat gacgatggac tgggttgttc gtgccatttt ctttgtcatt
     7261 cgctatcgaa gcgggaagtg gcagaatcag atgtgatgaa aaatacggaa tgttttgggg
     7321 agagagaaag aaaaaacgat acatctggag gaagttatta ttatgaaaaa cgcaaagaaa
     7381 ctgattcccg gagcggcgat tgcgctggtg attgccgctg cggcgaaatt cctggaaagc
     7441 ctggaggaga gcgccggact gcactttatc ggggcatcgg tcatcgccat gtttatcggt
     7501 atgattgtga atgcctttta caagccgaac agcgtgacag cgccgggaat taaatttaca
     7561 tcgaagaaga tcctgaaatt tgcgattatt ctgctcggtg cgagcctgaa tatccgcacg
     7621 gtgctgacgg tcggaagatt ttccctgacg gttatggtat ttaccctggc aacgtgcttt
     7681 ggcctcggcg cactgatcgg aaaagcactt ggactgaact ggaagacgag cagcctcatc
     7741 aacgccggca cgggtatctg cggcggttcc gcgatcgcgg caatcgcgcc ggtcatcgag
     7801 gcgaccgata tggacattgc ctatggcctc tccgcaacgt ttttatttga tacggtcatg
     7861 attgtcgtat tcccgattct cggtcactgg atgggccttt cagatgccgc gtttggcctc
     7921 tgggccggaa ctgcggtcaa cgatacctca agcgtcgttg ccaccggtta cgcattcagc
     7981 gaggctgccg gtgatttcgc aaccatggta aaactgacac ggacccttgc catcattccg
     8041 gctgttctgg cattcgccgc catcaacgta catctgaaac ggaaggcgca ggcagcagaa
     8101 ggaaccgccg taaaggtaag catcaaatcc atttttccat ggtttatctt aggctttctc
     8161 gccatgtcag cactgacctc cttaggtctt atcccggcag gcacagcagc cgcattaaaa
     8221 accatcagca aattcctgat ggtagccgcc cttgcggcca tcggcctgaa caccgatttc
     8281 aaatccctct gccgctccgg cgcaaagccg atgctgcacg gattcatcat ttcgctgctg
     8341 gtagtcctgg tagcgattgc agtcgaattt gcgatcggca ttgcgccgca tggggtactg
     8401 taaagaaaca gagaattaaa aatccaggca tccatatagg atgcctggat tttttgaatc
     8461 aaaacgggtt tgtcgtttcc ttcaaaaacg ttttcaaaac ctgcagcacc ccgttctccc
     8521 agtacggcgg gcaggtgtgt tttgctgagg cgatgacttc cgctcgggcg ttggagacgg
     8581 cgtagctttc gccggcgttc tgaagcatct cgatgtcatt caggttgtcg ccgaagcagc
     8641 agacttcgtc tgaggtaagg ccgaagtagt tcatcagaaa tgagagaccg gtccatttgc
     8701 tgacgccgcg ggcgttgcag tccaaccatt ccttaccggc gacagcgagt tttgcctgat
     8761 gattccacgc cgggatgaaa tttggggagc aaagctcttc acaggaggtt ggatggtaga
     8821 ttgtgaattt gataatgtcc tcgttttcta ccttcgacag atccggtact tcgcggacat
     8881 cgaagccgta ggagtcgcgg agccagtgaa acatgcggct tccggcatct tcggcgaggc
     8941 agtagtccgg ggtggcggca aagcagtcgc aggacggaag ttcgtcgtgt accatgcggc
     9001 acatggattt ccagagtgcc gtttccatcg gatatgtttt caaaatcttt tccggtgtgc
     9061 gcacgacagt tccgccatcg gagatgtaga gcaggcggtc cttgatcgga gcgaaaagct
     9121 tctgttcact ggaaaactgg cggccggaac agacaacaaa atagatgccc tggtcgacaa
     9181 gcctggaaac aacagtcata tattccgggt cgatcagaag tgtgccgtct ttgacgagcg
     9241 ttccatcgac atcggttgcg atcaacttta tcataataga aaatctcctt atcttgtcac
     9301 agaaaaaatt caaactatag caagaccaga aaataacgca aaaaaggcag tgctgcaggg
     9361 cgcgaattac aaggaacgga tttgaaggcg taccggggta cgacgaaaat ccgtgacgca
     9421 gtaattcacg ttctgcagtg ctgaattttt tgcattattt tctgggattg ctataaacat
     9481 gattatatcg tgaattgctt cggggtgcaa gccggaaccg gaggaacgca aaactccacg
     9541 ttgacgaaac gcagaaataa ggcgtaaaat aaagaaaaat atatatccac agggggattt
     9601 ttattatggc atttattcag gtaagcttca tgtcaaaaac tttaatgcgc accgtaccgc
     9661 tgcaggttat tctgccggtc gacaaattta cctttgggca gccggagcgc gaggaaaaac
     9721 cgtttaaaac gctgtatttg ctgcatggca tttttgggaa tgagacagac tgggttcatg
     9781 gaaccagaat ccaaagatgg gcagaggaga aaaaccttgc cgttgtcatg ccggccggcg
     9841 agaatgcgtt ttatgtggat cagccgtcca tcggtgccat gcacggacag tttatcggag
     9901 aagaactggt tgagattaca agaaaaatgt ttccgctgtc cagaaaaaga gaagacacct
     9961 ttatcggagg cctttccatg ggtggttttg gcgcactcag aaatggcctg aaatatcacg
    10021 ataccttcgg ggctgtgatc tgcctgtccg gtgcacttca tgtgctggaa aatccggaag
    10081 agagcagagc ggacagcttt gcacatgaag aaggttattt cggaaatctg gtcgaagcag
    10141 caaagagtga caaaaacccg gctgttctta tcgagcagtt aaaggaagca cggaaaaaag
    10201 atccgtctgt caatgtgccg accgtattcc aggcgtgcgg aacagaggac ggacttctgg
    10261 aagtaaaccg tctctaccat aaacggatgg aagagagcgg catggacatt gtctactatg
    10321 agagcaaggg tggccatgaa tgggatttct gggatgagtg gatcaaaaag gcactggact
    10381 ggctgccact tgaggaaaag acggaaggtc tgaacagcgg aaacgtaaat gtttaaagga
    10441 aaaagggaga caggcaatgc cagaaacgag aaatgacgaa ttgtaccgtg cgttaaaaca
    10501 caacggttac ccggacgact tctgccggga aattgcatac cgccagatga acaccgattt
    10561 taccgccacg cggatgctcg gctatctcta ccgcgtcaca agcccgcgcg ccgaggacgt
    10621 tgtcgatgag atgctggcta tcctgagtga ccgcaaagca tggattgaga aaaaacagtc
    10681 tgaggaagcc caggcggcga tcagcagaat gtaccgggaa ggcctgtaac ctgggcgggc
    10741 gtttatgcag aaaaagcaga aggaacgggc ttgcattctt ttccggtttc tgctacaatc
    10801 agtgcagtga aaaagcgacg aaaagaaaca gtagcgacag agcgcattca gagagaaagc
    10861 ggcaggtgaa agctttttgc agaatctatc gtgaaccggt cttggagctg tgcaccgaag
    10921 agaagaaaat ccatcaggga aaatttattt cgtaggctgc accgggatct cccgttacag
    10981 agaaggtaca gagagcagag atcacgcgta ttacaaaata caaaaatcgt ggggtacaaa
    11041 tgctctgtgt cagagtgcag gagttttcct gaatttaggt ggtaccgcgg ataaaaaaat
    11101 tcgccctaaa ctgcagcttg cggtttaggg cttttttctg tttgaaaacg aagcatctaa
    11161 ctcgttactt cagtgatgag ttagatgcgt aaaatgttga ctaaaagctt aaaatcggac
    11221 aggctggaat gttctcgcgt ctggaagact tcagatgttg aaaacccaaa gaataaggag
    11281 gaaatcagaa aatgaaactt gaaaaattag taggagagcg cttcaaagaa agacctgctg
    11341 actgtgtcat cgacagccat gctattatgg taaaaggcgg atacatcaaa tatatggcaa
    11401 acggaatcta ttcttcctat ctgccgctgc gccgcattgt gcgcaaaatc gagcagatcc
    11461 tgcgcgagga gatggacaaa atcgacggcc aggaagtgca gttcccggtt gtcatgccgg
    11521 cttctctgtg ggatgagtcc ggccgttacg atagcatcgg cgatgagctg ctgcgcttca
    11581 ccgaccgcaa caatgccaaa atggtactcg gcatgaccca cgaggaggca gccgtacacc
    11641 ttgtacgcga gtatgcacag agctacacca aatatccgtt tatgatctat cagatccaga
    11701 caaaattccg tgatgaggcc cgtccgagag ccggactgat ccgtgtgcgt gagtttacga
    11761 tgaaggatgc ttactccttc cataccagcc aggaagatct ggagcagtat tacgagaaat
    11821 gccacgcagc atacgagcgc atcttcgagc gtgtcggcgt gccggaggtg gtatctgtca
    11881 aatccgattc cggtatgatg ggcggaaaca tctcccatga gttcatgctt ctgactccgg
    11941 tcggagagga ttccatcgtt ctgtgtgatt cctgcgatta cagagccaac atggaggcag
    12001 ctgagaacat ttccgatatc gcaagagacg cagaatccgc tgcactcgaa aaggtttata
    12061 caccgaacgt ccacaccatt gaagacgtct gcaatttctt cggagacgag acaaagaact
    12121 cctgcaaagc cgttgtttat cagcagaatg ttgatgataa atacgttgtc cttttcatcc
    12181 gcggtgatct cgaagtcaac gagacgaagc tggtcaactt cctcggcgag caggttcacg
    12241 cagccgtgat caccgaagaa tgcggactga acgcaggcta tatcggaccg gtgaacctga
    12301 aggtgaacgg agatgccgtt gttctctatg ataaatccct cgagggaaga aacaaccttt
    12361 cctgcggagc caacgaggca gagcatcatt acaaaggact cgacatggag cgcgacgttc
    12421 cgaatgccga gtatcacgat tttgcaaaaa tccaggaggg cggcatctgc ccgaaatgcg
    12481 gcaaaaagac cgtgaagatc tccagaggta tcgaggtcgg aaatatcttc cagcttggaa
    12541 ccaaatatac aaaatccatg aatatgacct atgtcgatgc aaacggcgag agcaagaccc
    12601 cgatcatggg atgctacggt atcggtgtcg gccgtctggc agcatcggtc tgcgaggcac
    12661 atcacgatga gtacggcccg atctggccga aagctatcgc tccgtggcag gtacacctct
    12721 gtgcagtccg cgtcgatgac gaggaagtca gagcatatgc ggacaagctg tacgaggatc
    12781 tgcagaatgc aggcatcgaa gtgatctacg acgacagaag cgtcagagcc ggcgtaatgt
    12841 ttgccgatgc cgacctgctc ggcattccgc tgcgcatcat cgtaagcccg aaaaacatga
    12901 aacagggcgt tgtcgaggtt gcatcccgcg ataagactct gaaaacacaa attccgctgg
    12961 aaaatgtaat ggaagagatt aaacagtatc tgtaatgatt cagaaccatg cgaaagggaa
    13021 gaaaaatcgc gatggggctc ggaagaagtt cgacaggaga aaacacgata tggaactctg
    13081 ggatatctat gatgaaaaca aacagccgac cggccgcacg atgaaacgga atgactggca
    13141 tatgaagccg ggcgagtttc acctgaccgt actcggcgta atcgcaagac cggatgggac
    13201 ctttcttatc acaaaacgtg tcatgacgaa agcctgggca ccgggctggt gggaggtttc
    13261 cggcggcgca gcacaggcag gagagagctc ctgggaggcc gtgcgccgtg aggtcagaga
    13321 ggaaaccgga ctcgatgtga gcaaggctga cggcggttat ctctttacct accggcgcat
    13381 caacccggat gagggagaca attactttgt cgatgtttat cgcttcgtga tggatgtcaa
    13441 agacgaagat ctccatcttc aggaggaaga aacagacggc taccgttttg ctacgttgga
    13501 cgaaatcaaa gggtacgccg cagaaggtaa attcctgcat tacgacagca tccgccaggc
    13561 atttgaactg taacagaaaa ataagataag aaaaacgcgt tcccgccgga aaccacaggg
    13621 gtttccgaaa acgggaacgc gttttcttag ccttagagcg tgtctgaaaa aggcttttcg
    13681 caagcagttt attgcagtta attgcgattg aatacttcgc ggttgagcgc cttttcctga
    13741 tggctgacaa tcgttgtgca gacagcatcg cctgtgatgt tgactgcggt acgcagcata
    13801 tcgagaatac ggtcgatacc catgataaga gcgataccct ccgttggcag tccgacggaa
    13861 ttcagtacca tagccagggt aacaaggccg acacttggga taccggcggt accgatggaa
    13921 gccagtgttg cggtaacaac aaccgttgcg aggtttgccg gggtcagatg aataccgtac
    13981 gcctctgcga tgaagatgac agcgacaccc tgcataatgg aggtaccatc catgttgatg
    14041 gttgcgccga gcggaatggt gaacgaagag atctgtttcg agacacccat ttttttgttc
    14101 agggtatcaa tcgacatcgg gattgttgcg ttggaggttg cggtggaaaa tgcaaatccc
    14161 ataaccggaa gaaatttctt cacgaattta atcggattca gccgtgtgaa tacgaacaga
    14221 agaacctgat aaatgacgaa gcactgcagt gccagcgcca gtgttacatt tcccatgtat
    14281 ttcagcatcg gtgcgaacgc agaaaagccg accgttgcaa aggtacgtgc aatcagacag
    14341 aaaacgccga ccggggccac cttcatgacc gtcatggtca tttccatcat gacatcgtta
    14401 aactggctga taaagttgga aacgaccgaa gcgcggtttc cgagctttgc cagcattacg
    14461 ccgacaaaca gggcgaatac gatgatggga agcatatcgc cgtttgccat ggaaccaatc
    14521 gggttcttcg ggatgatgtt gagcagtgta tccaccaggc tgacgctgga atctgctgct
    14581 gtcgaggaca ggtcggcttt ctgaaccgca ctcatgttaa gtccgagccc cgggtgaatg
    14641 agcagggcgg ttccaagtgc gatgcagacg gcaagtgctg tggtaacaag gtaaaagccg
    14701 atcgttttta cgccgacctt tccgagggtt ttgctgtctc cgatggacat acttccgcag
    14761 acgagggagc agaagacaag cggtacgaca agcatctgca tcaggcggat aaagccctgt
    14821 ccgatgacat acagcacacc ttctacgatg acggtgtcct tgatatagct ggacgggatc
    14881 caatagtgaa tcaccacgcc gagcagcgca ccggccagca gggcaataaa aatctgggtg
    14941 gaaagtccgg gcttcttttt tgacttttcc gttgtttctt ttctgtttcg gaccattatt
    15001 cgattcctcc ggtttcttcg agatattctt tcagtgccgt tttccagtcg ggcggttcag
    15061 gaataccggt caggcggagc atcatgttgt ccagaacaga ataggtcggg cggacatcat
    15121 cgcgtgcgac aaccgggtag agatcaaggt caccggtttt tccggtatac tcgaggatgg
    15181 ttcttgcaaa ctcatagcgg ctgcagcttc cggggcagac aacgtggtac agaccatact
    15241 cctcgttgtc gataaagtgg cagatcacgc gcgcaagctc aaaagcactg gtcggagccg
    15301 catactggtt gtttggaacc tccatcgtgc cgccttttgc cgcattgcgg agtacttcat
    15361 ccacaaaatc gcggccgatc ccgtaaatcc aggagcttcg gatgatgaca aagcggttca
    15421 aaagctgtgt cagaattttc tctccggcct cttttgattt tccgtagatc gtacgcggat
    15481 gcacggtatc aaactcgttg tacgggatgc gggattcctt gtcgaagacg tcatccgttg
    15541 agatctgaat aacctttgcg tcgatatcat tggcggcaag cgctacgttg cgcacaccga
    15601 tggcattgac acgataggct tcgtcgatgt ttttttcaca ctcggcaacg tcggtcatac
    15661 cggcgcagtt gatgataacg tcgggacggt tgacataaac gaactgtgtg acttcatcga
    15721 ttttggtaat atcaacctcg tgaatatccg ttggaagaag ctgatattcc acaccctcca
    15781 gcagttccag aagggcagta ccgatgtggc cttcggcacc tgtaatccat atttttttca
    15841 taggttctct cctgtccttg atttatatac agaaaaactg taattgtatt tattttacct
    15901 gtggacttta cagaagtcaa taaattatgg aaagtctctg tgcagaaccg gaaaaaagtg
    15961 gtatcatttc tgtatatgtc tgcggtgtaa gactgcatat ctgctacaaa agaaggagga
    16021 gtatttttga aaaagaacaa atatgaaatc gacatgtgca acggcacgat tatggacaag
    16081 ctgatttcct tttcgctgcc gctgatggtg tccggcattc tgcagctgat gttcaacgcg
    16141 gtggatatca tcgtcgtcgg acggttcagc ggaagccagg cgcttgcggc tgtcggatcg
    16201 acaacggcac tcatcaacgt gttcatcaac ctgtttatgg gaatctcgct cggtgcgaat
    16261 gtgcttgcgg cgcggtatta tgcagcgggg cgcgaccggg agatgtcgga aacggtacat
    16321 acatcgatca tgctggcgct gatcagcggc gttgtgatgg cgtttgtggg cgtatttttt
    16381 gcaaaaggcg cgctggagct gatggataca ccggcggatg tcattgatca gtcggcattg
    16441 tatatgagaa tttactttat gggaatgccg tttttcatgc tgtacaacta cggtgcggcg
    16501 attctgcgtg ccgtcggcga cacgaagcgg ccgctgtatt tcctgattgt atcgggtgtg
    16561 atcaacgctg gtctgaatat gttcctcgtg atcgtgttca gtctcgacgt ggcaggtgtg
    16621 gcgattgcaa cggtggtttc gcaggcgatc tcctgcgtgc tggttctgcg gtgcctctat
    16681 aagagcgagg gaagttatca gctccggttt tcgaagctgt gcctgaaaaa ggattacatc
    16741 atcccgattt tccaggtcgg catcccggcg ggtattcaga gtaccgtcat caacttctcg
    16801 aacgtgctgc tgcagtcatc cgtcaattcc ttcggttcga ttgcaatggc aggctacacg
    16861 gcggccaaca atgtgctggg atttttatac acctcagtca actccgtcac ccaggcgtgc
    16921 atgagcttca caagccagaa ctacggtgtc ggaaaataca aacgaatgga taaggtgctg
    16981 atcgactgcc tgattctctc cagcggcatg gcactggtga tgggtggcgg cgcgtatctg
    17041 ttcggtacgg aaatcctgca gatttacacc ggggacccgg aggttatccg ctgcggtatg
    17101 gaaattctgt cgatcacgac ggttccgtat ttcctctgcg gcatcatgga cctcttcccg
    17161 ggagccctgc gcggtatggg atgctcggcg gtgccgatga ttctgtccgt catcggaacg
    17221 gtcggaacgc gaatcgtgtg gatcttcggc attttcccgc agcaccgctc gctgtatgtg
    17281 ctgttcattt cgtatccggg atcgtggatc atcacgatta tcatgcaggt gatctgctat
    17341 ctgattatgc ggaagaaagt ccacagccgg gcagcagccg tataaaaaag agaaaaaaga
    17401 caaaaagcca gaaaaaccgc ggataaatcc atttttaggt ggaattatcc gcggttgtgt
    17461 gataagatgg ctgtatgcga agaggcaggt agaagataca gggacacctg cctcttactc
    17521 tgtgaaaaag gagagaagag agaacatatg aaaaagtttt atgcatggta tcagaagcat
    17581 attacaggtg cgatctttac cggactgatt ctcggtatct taaccggact tcttctggcg
    17641 gaccgcttta cgccgattct gaccgtaacc ggcgtcatcg gcggcatcta catgaacgcc
    17701 ttgaatatga tgattttccc gatggtattc tgctcaattg tcatgggtat ctgcagcatc
    17761 ggaaatgcaa aaacgacagg taagatcacc ggctactcga tgattttctt cctgtgcacc
    17821 accgcgattg caagtgcagt cggcattatc attccaagac tgatccgcct cggaaaggga
    17881 gtccattttg agatggcaac ttccgatatc caggcgaccg agatgaccag catcctcgac
    17941 acgatcaaaa acctgatccc gtccaacccg gtgaaggcgt ttgcggaggg caacatgctc
    18001 caggtactcg tgttcgctgt cgttgtcggc tttacgctga ttgcggtcgg agagaaggga
    18061 cagccgctct taaacgtgat cgactccctg aatgaggtct gtttaaaagt catcagcacc
    18121 gttatgtact tcaccccgat cggtgtcttc tgtacgatcg tgccggttgt cgaggcaaac
    18181 ggaaccaaaa ccatcgtttc cctggcaacc cagctcatca ttctgtatgt ggcattctat
    18241 ggatttgcga tcgtcgtcta cggcggcgct gtaaaactca tcggaaagac gagtccggtc
    18301 aagttcttta aggcgatcat gccggcagcc ctgaatgcat tcggaacatg ttccagttcc
    18361 gcgacgatcc caatcagcaa gcgctgtgtc gagaaagaac tcggcgtttc gaaccagatc
    18421 agcagtatcg caatcccgct cggtgccacc gtcaacatgg atgcggtatc gatcctgatg
    18481 tcgtttatga tcatgttctt cgcaaatgcc tgcggcatca acgtcagcat ttcgatgatg
    18541 gtgatcattc tgcttgccaa cgtcctgctt tctgtcggaa caccgggtat cccgggcggc
    18601 gcgatcgcat cattcgcagc actcgccacg atggcaggtc ttccggccgg cgtcatgggc
    18661 gtttacatca gcatcaacac actgtgtgac atgggtgcca cctgcgtcaa cgtcatcggc
    18721 gatatggcag gctgcgttgt gctgcaggag aaaattaaac tggaagaaaa ctaaaaaatg
    18781 ttacatagaa aaagagggat tcctttcacg ggagtccctc ttttgcaatg cgtaaaataa
    18841 attaaatata gtccgaaaca tatggaatat ggtggaagag caggtcatcc aggcattcca
    18901 cagtctgcgg cgtcgtttcg atgcgcgcca tctcatacag cttggaagcc gtcttgtagc
    18961 cgagcagcag tgtcgtgagc gatgcgatcg tcattttcat cacatggtcc ggctcgcggt
    19021 cggtcggcac acagtgtcct ttttcaaaga agaatgtgaa gctgtcattg ttccacggaa
    19081 gcagctcatc ctcgatctca aaacggatac acacatccgg ctcgtccggg tcgcaggcgt
    19141 aatgttccat aaactgcacg acatcgatga tacgccccat cgagtacggg cgaatcgttt
    19201 ctttgatatc gctgtcctca agctcgaacg cgatgggttc actgtagtag ttatttcctt
    19261 tgacctcatc gatcatggaa tcgtgcgcat gaatatactc ccagagtccc agctgcgctt
    19321 cgcggttcag atagatcatc tctttgatat gcatgatatc ggagctgatc agatagacca
    19381 tgtagccgta cggcacatca tcagcggaat agtagatcgc aacgacggta tcatcctcat
    19441 cccagcggaa atattcttcc cacgccagat tgttgcggta gaggcagccg tgcgtcttcg
    19501 aagcaaattt ggtatgtagt tccttgaaat ccttgtcatc ccatgccacg cggcgcacat
    19561 agccaggggc tttgagtttc cgcggcacct gcgtgtcctt gatggtgtac gtcatcttat
    19621 tggaaatgat ttcccatccg agattccggt agagcggaat agagtaggga tagagaagcg
    19681 caaaggaacg gtggttttcc cgcatgcgcg tgagactccg gatcatcagc tttttcatga
    19741 tgccctggcc ggtgtattcc gggtaggtgc agacgctggt gacaaaaccg acgggatagc
    19801 ggacaccgta gatattcatg tccagcggat aaaccgcgaa ctgtgatacc agatcctcct
    19861 cgcagaaaca gccgagcaca tcggcacgga cgagaaccgg gaatttggac tgcttgattt
    19921 catcgtcttt ccatccgctt tcggaaagct cctgctctgt aacctgaaag gcgtagcgca
    19981 gaagtgcatt gtactgctct gcgtcgtcga tggtcagcgg gcggaaggtt aagttcgaat
    20041 ctttcgtttg aatcatggat tactccctca ttttattgtg aatcaaagta tagtgaatca
    20101 gatactttat tttttcgtaa atattctatc ataagaaatg gaatttgtgc agttaaaaat
    20161 aaagcccatt ccgcaaattt ggtaaaaaga tcaaaatagt gttagaaatc ggataaaaac
    20221 ttcaagaaac agacaaaaaa atctaataaa atgaaaaaca tacagggaca gtctgcgcga
    20281 aaaagcagac atacgatttc cagggtgact gcgttgagat cgacgtgttc aatccggaga
    20341 caagaaaata tgtctgggac gtatgcaaga agaactacta cgattacggc atcaatgctt
    20401 tctggctgga taactccgag ccggattacg gtgtttacga tttcgatcat taccgctata
    20461 ttgaaggacc ggcgctctcc tgcagcaaca tctatccgca gttatacagc cgtgtcttct
    20521 atgataatat gaaagattta ggcgacgttc cgagtacctt tgaagctttc tacgatcagt
    20581 tacaggcagg tcttaacatg ggtcttgccg gaattccgtg gtggacaacc gatatcggcg
    20641 gtttcatgac agacgatgtc aacgatccgg acttccagca gcttctgatc cgctggtatg
    20701 agtttgcagt atattccgct gtcctccgta tgcacggcga ccgcggccca tacaacattc
    20761 cgatgctcga cgaccgtgat ttcggcggcg gctgcctgca cactggccag ccgaacgagc
    20821 tctggagcta cggcgaagag aactataaga tcatgaagaa atactatgat atccggatcg
    20881 agatgcatga ttacatcaaa aaactgtacg acgaggcatc cgagaacgta caagggacgg
    20941 aaaagtctat gaaggcggcc agacgatcca cgccgatgca ccgatcgatt cgattccggt
    21001 atttaaacga aaatagttaa gtaagaagaa aaaaccgacc gcgtaaaacc ggtcggtttt
    21061 tttgaataaa tttccattac aggacggttt ttgtacactt ttttcttgac gaaacgggaa
    21121 acaatcgtta cactattatg tataactgta attaaaatgg gaaaatgtag caaaacgcgg
    21181 aagcacacaa aggggtaatg aagttttgtg tgtaccgggg agaagagagg gaaacagatg
    21241 aatcagaccg aattgccgga atgtgaaaaa ataaataaga aagagccttc taaggatgtt
    21301 cagatgacac agaaacaggc agaacgtctg gtggaacatc tgaagaatat tttcatgtat
    21361 gtgcgggttc tccgtccgga tgagatcgga gattttatta tcgatgaaga gaatggcaag
    21421 ctgtgtgaat gctacacagt atggaacaaa gcgatgccgt gccttaactg tatttccgaa
    21481 aaagccctgc gggagaaatc gcagaaatca aaactggaat gcgttgcgtc gaatgtgtat
    21541 caggtcattg caagatacgt ggagatcgaa ggagaaccat gcgtgatcga gatgatcaac
    21601 cgtctcgacg atgaaacact gatggactcc gaaggacgcc agaatctggt aagcaagctg
    21661 aacagctaca gcgaagagct gtatcgtgac gcgctgaccg gagtattcaa ccgccgctac
    21721 ttcgaagacc agatccggga cgccagcttt tgctgcggcg tagccatgat cgatctcgat
    21781 gatttcaaat tatacaacga tacatacggc cacaatgccg gagatatggc gcttgacacc
    21841 attgtaaaga ccgtcaaccg ctgcacccga cgtaccgacc gtctgatccg cttaggagga
    21901 gacgaatttc tgctggttct gccggagatg gatgaggaaa actttgtaca gaaattaaaa
    21961 cagattcagt cacagatcca tgaggcacag gtgccgggct actcgaagat gcgtctttcc
    22021 gtcagcgtag gaggtgttct gacaagagat gagacgatcg gcgaagctgc gatgcgtgcc
    22081 gaccagctga tgtatcttgc aaaacaacgg aaaaacatgg ttgtcaccga gaaagaccag
    22141 atcctgggtt gtcaggacga tgttgtccgt caggaaaacc gcagagaaaa acagcgcatc
    22201 ctgatcgtgg atgattccga gatgaaccgt atcatcctct cggagattct gcaggatgaa
    22261 tacgatatca tcgaggcttc caccggcgaa gagtgcctgc gtctgctgga agaatacgga
    22321 acaggccttg cgctgatcct tctggatatc gtcatgccgg gcatggacgg tttcggagtg
    22381 cttgactaca tgaaccgcaa ccactggatc gaagaggttc cggtcatcat gatatcgagc
    22441 gaggattcca ccacatttgt gcgccgtgcg tatgagcagg gcgtatccga ttatatcagt
    22501 cgtccgtttg atgcaaaagt tgtttaccag cgtgtcttca atacgatcaa attatatgca
    22561 aaacagcgcc gtctcgtgac gctcgtcacc gagcagattt acgaaaaaga gaaaaacaat
    22621 cagatgatga tcagtatcct gagccatatc atggaattcc ggaatggcga gagcggactt
    22681 catgtccacc acatcaacgt tctgaccagt atgctcctcg agcgtctggt acagaagaca
    22741 gaccgctacc acttaagctg gtccgatcag ctgatgatca caacggcatc cgcgctgcac
    22801 gatatcggaa agatgggcat tgacgagaac attttaaata agccgggaag gctgacgaaa
    22861 gaagagttcg aggagatgaa gaaacatacc ctgatcggag cgtccatgct gaagagcctg
    22921 gaactgtatc aggacgagaa gctggtacag attgcgtacc agatctgtcg ctggcatcat
    22981 gagcggtacg acggaaaggg ttacccggac ggcttaaaag gtgaggagat cccgattgca
    23041 gcccaggttg tatcgattgc cgatgtttac gatgcgctga caagcgagcg tgtctacaaa
    23101 tcggcataca cgcacgagaa agccatcgag atgatcctga acggcgagtg cggtaccttc
    23161 agcccgctga tgctcgagtg tctgctcgat atcaaagatg ccatctgcga ggagctgaac
    23221 accatatccg aagaaaaaca gacttcgatt cagattgaaa agctgaaaaa gaatctgaaa
    23281 tcgggggggg ggcagagctg agctttctga actctgatga gaagttatcc acagaagatg
    23341 cctgggagaa caggtatcat gcggattcga aagacgatct taaataaaaa tataccaaaa
    23401 cccatggatt tctgaaaaga ttccatgggc ttttcgcact tttgtgcaaa tttttgttgt
    23461 gattccggag gacaactgct ataataacag aaaatgtcaa aaaatacgac aaaaataccg
    23521 gaagagggct gtgcgaacaa gtacacacaa gtctggaaag gataaatatc atgaatcagt
    23581 tggaagtatt acgagagagc cttggacagt gcgatgagat tattttggat gcgctgctta
    23641 tgagaaaccg tattgtcgag gacattatgg tatataaaga agcaaataac gtgccgattc
    23701 tccagccgga gcaggaagca aaacagaagg aatggctgga acgccggatg caggaaaaac
    23761 gccataaaaa cgaagtggcg gatgttttcg agagcatcac aagaaacagt aaaaagattc
    23821 aggcaagaaa gctgttcaat tataatattg tactgattgg cttcatggga gccggaaaga
    23881 gcaccatttc cgattatctc aaaaccgtgt ttgccatgga tatcatcgag atggatcaga
    23941 tcatcgcaga gcgtgccggt atgagcatct ccgatatttt cgagacctac ggcgaacagt
    24001 atttccgtga ctgcgagacg aatcttctga tcgaaatgca gtcgagaaca aacgttgtca
    24061 tttcgtgcgg cggcggaact ccgatgcgtg agtgcaacgt tgtagagatg aaaaagaacg
    24121 gccgcgttgt tcttctgact gcaaagccgg agacgatttt agaccgtgtc aaagacagcc
    24181 acgacagacc gctgattgag aacaacaaaa ccgttccgtt tatcgcagat ctgatgagca
    24241 agcgccgtga gaaatatgag gccgcagccg atattatcat cgagacggac ggaaagagcg
    24301 agctggagat ctgcgaggag ctgatccaca gcctgcggca gatggatgca aaagcggaat
    24361 aaaagagaaa agcaagggaa aaaggaaaag agactgctgt gtgtaacgga ggtctctttc
    24421 ttttttgcgt aaccgtgaca caaaaaacag aaaacgcaaa atttacggga aaaggattga
    24481 caaaacagat atttcccgat attctgataa aagttgggct aaaatcgcat gaaaaaagcg
    24541 aaaaaacaag gtattattgt ccgaacaaga gaaaaaattc aggataatta tatgaaatac
    24601 aatggcccgt gtgcagacac agccggatct gccggggaag gtctgcggaa aaaaggagga
    24661 acatcaatga gaaaaagaaa caaagtcgtt tctctgcttc tggcaggatg catggcagcc
    24721 ggtcttctgg ccggatgcgg aagcagcgga gagacacagg tagcaactgc tgacaatgcc
    24781 acggcggcat caaccagtac tggagcagca gattcagcag caccggagac tgcaggcgaa
    24841 gtggacggac agatcaatct tcgtgcagta gcctttggaa acaatttcga tgtgcaggat
    24901 atgggatggc gctggatgat gggagagtgc tacgaaggac tgatgcgtga cgtagcagat
    24961 gaaaacggag accgtttcga gtatgccggt gcagaatcca tggacgtatc cgatgacgga
    25021 ctggtttaca ctttccatct gagaaaggat gccaaatggt ccgacggaca gccggttacc
    25081 gcagcagatt atgagtacgg ctggaaacgt ctgttaaatc cggaatacgg ctattcctat
    25141 tctttctacc tgttcaacgt agtaggagca gaggagtatt ataacggaca gggctctgcc
    25201 gatgagatcg gaatcaaggc agtggacgat tacaccttcg aggttacctt aaaggtagcg
    25261 gatccgacct tccagtccaa gctggtagca actccgcttt acccgacccg tcaggatgtc
    25321 gcagaggcag ccggagacca gtggggaaaa gactggacaa aatgtgtcta caacggaccg
    25381 tttgccatga ccaacctggt agaagataac tccatgacat gggtgaaaaa tgaccagtac
    25441 tgggataagg acaacgtaaa actgaatcag gtaaactggt actgtgtagc agagaacgca
    25501 acagcagcaa ccatgttcga taacggacag ctggacgttt tcgatgcagc aggcgattac
    25561 attgcaaaat atgacaaaga agtagaagca ggcaatatgc agaccatgac caccgagtac
    25621 ccgggaacta tggttctgtg ctacgagcag tctgagggcg gaaaatccgg tctgatgaaa
    25681 aacgtaaaca tccgcaaggc aatttcctac tccatcaacc gtgaggagat ggtatcagct
    25741 gtttacggaa gatacaccgc agcttacggc ttcgtttccc cggcgatcac gctcgacgga
    25801 acttcctacc gcaagcaggc gtcggagacc atgaaagagg aatatgagca gtatgcaggc
    25861 gatgcagaca agttaaaagc actgttccag aaaggccttg atgagcttgg tatcaccgac
    25921 aaaccggaag acatcaccat tacactgctg tcccagggtt ctgctaccga gaatcagctc
    25981 gagagagagt acctgcagca gtccatttcc cagaatctgg gcgtaaaggt agagctgaac
    26041 accgttggcg attaccagat gtttgcaaac gagcgcgata acaagaacta cgatatcttt
    26101 gtaggcggat ggttcagcga ttacaacgat ccgctggatt tcctgaacgt catgaagaca
    26161 ggcgtttatg attcctacgg cctctatagc aacagcgagt acgacagcct gatcgattct
    26221 ctgaccggag agaacgacaa cgcaaaacgc cttgagattt accagaagct ggaagatctg
    26281 ctggttgcac aggattgtgg tgttgcaccg ctgtactact ctgataaaca ttactattat
    26341 cagaactggg taaaagattt ctacacatcc tccttcggcg caagccagga gctttacaga
    26401 gcatctgtac agaaataaaa aagcagcaaa acagaagaaa tgagggaaga agggcttatg
    26461 aaacaatagg ctcttctttc gctatcataa taaaattccg gtcggaaaag accggacagg
    26521 gagaggattt ttatgccaaa atatttactg cgccgttttg cagaaatgct gatcacactg
    26581 tttctgattg caacggccac gtttttcctt ctggaagcgg ttccgggaga tccgctgacg
    26641 gagcgtgcga gcaagctctc gccgaccgta aaggaaaacc tctataagcg ctacggcctc
    26701 gacaagccga tgatggagcg ctatgtgatc acaatgaagg gaatctgcaa gggagagttc
    26761 ggagaatctg ttgtctatgc gggacagacg gtacagaagc tgattcatga ccgtcttccg
    26821 gtatccgccc gccttggtat ccagcaggtg ctggtcggta tcacgatcgg tctgctgctt
    26881 ggaattcttg cggcaatgag gaggggaagc ttctgggact atatggtgat cgccctgtcg
    26941 gtcctcttga tttccattcc gaacctgatc tttggtctga tgcttcagaa aattttcgca
    27001 ggcaacctga aggttctgcc gacgatcggc tggcctgcgg gaaaggatct gtggttcgga
    27061 ggatgggaat acacgattct tccgaccctg acaggatgct tttcttatat cgcaacgtat
    27121 gcgcgtctgc tgaaaacctc gatgctcgat gtgatcaatc aggattacgt gctgactgct
    27181 gaatcgaagg gcttaagccg cggacagatt atccgccgcc atatccttcg gaactcgttt
    27241 atcccggtta tcacacagct tccgatgtcg gttgccatgt gtatcacagg ctccttcttc
    27301 atcgagagca tcttttccat cccgggaatc ggacagtatt atgtgacggc ggtcaataac
    27361 caggatcttt ccattatcat gggagagacg gttattatcg cagcacttta catcggtgtt
    27421 ctctttatca cggacctgct gtacgtggtc gttgacccga gaatcagtct gacaggaaag
    27481 agcagataag gaggatatta tggcagcatt aacaccggaa aaatttaaag taataggata
    27541 caccgaggaa gatgcgaacc ggattgaccg cccgaccatt tcctactggc aggacgcctg
    27601 gagacggctc cgcaaaaacc cgatcgcaat gggatctctt gttgtgctcg gcattctgct
    27661 tgtcatggtc atcatcggac cgcatatcaa aggatatgat tatgtatcca tgaatatcgc
    27721 cgagaaaaac cagggcgtat cgtcaaaata ctggtttgga accgacaacc tgggacgtga
    27781 cctcttctca agagtctggg tcggcgcgcg ggcatcactg atcatcgcga tcgttgcaac
    27841 ggcaatcaaa ctcatcatcg gaacggtata tggcgcgctg atggcacatt tcggcggctg
    27901 ggtcgatgag gttctgatgc ggatcatcga ggttatcaac tcgattccgt ctctgcttct
    27961 tacgattctg atcatgatga ttcttggaaa taacctgttt gcacttctgg tcgcgcttag
    28021 tgtcaccgca tggtgtaaca cggcacgtca ggtgcgcggt atgatcaaac agctccgtga
    28081 gacggaatac gtttacgcgg cggaggtgct cggtgcaaaa ccgctccgca tcatcttaaa
    28141 acactatgta ccgaatatgc tgggtatcct gattctggat acggcgacgg caatcccgat
    28201 gtttattttt acggaggcag gtcttagctt cctcggcatc ggcctcaagg caccatcgat
    28261 cagccttggt gtcctgattt cgatgggaca gcagaacatg gatttctatc cgtcacagct
    28321 tctgttccca tgtctcgtgc tctgtatcat cgttatggca ttcaaccttc tgggagacgg
    28381 ccttcgcgat gcccttgatc cgagacttcg ccagtaataa gtgggagata acgatatgaa
    28441 cgaacaaaac aatccgattc tggaagtaaa aaacttaaga gtctcttatc acacctatgc
    28501 cggtgaagta aaggcagtcc gcggtgtaca gttttctctc gagaaaggtc aggcgctcgc
    28561 aattgtgggc gaatccggct gcggaaaatc tgtaacggcg aaatccatca tgggcctcat
    28621 caaggccccg cagggtgaga ttaaagagaa cagtgaaatt ctctatcatg gagagaatat
    28681 tctgaaatac aacaaaaaac agtggcagca ttacaaaggc ggcgagtgtg caatcatttt
    28741 ccaggatgcg ctggcttcct taaatccgac gatgcgtgtc ggaaaacaga ttatggaaaa
    28801 tctgatgggc cacaaaaaca tgacaaaaca ggaagcggca aaggaggctg tcgagatgct
    28861 gcgcatggtc ggcatcccgg agccggaaaa acgtgtcaga cagtatccgc acgaattttc
    28921 cggcggtatg cgtcagcgtg ttatgatcgc gattgcattt gcctgtgatc cgaaaattct
    28981 gatctgtgac gagccgacga ccgcgctcga tgtgacaatt cagggacaga ttctcgatat
    29041 tatcaagaat ctgcagaaga aaaatcagac atccgtcatc atgatcacac atgatctcgg
    29101 tgttgtggca aatatcgcac agaagattgc tgtcatgtat tccggcatca tcgtggagag
    29161 cggaacgtgt gaggacattt tctatcgtcc gcgtcacccg tatacctggg cgctgatccg
    29221 ttccgtaccg cgtctggatc tgaaaaacaa gcaggaactc gcgacaattc cgggaactcc
    29281 gccggatctt ttaaacccgc cggtcggatg tccgttctgc acgcggtgca gctactgtat
    29341 gccgatctgt aaggaagaga tgccggaggc aacggaattc ggcggcgagc ataaggctgc
    29401 ctgctggctc catcacccga tggcaccaaa agtagaaatg ccaaatctgg aaggaggaga
    29461 actgtaatgg aaaataacga aaaagaagtg cttttttccg tttcccatct gaaaaaatat
    29521 tttcaggtcg gaaaccatgc gaccttaaag gccgtggatg atgtctcctt cgaaatttac
    29581 aagggtgaga cacttggaat ggttggcgag tccggatgtg gaaagacgac gtgcggccgt
    29641 acctgcatcg gcctgtatga caagaccgac ggacaggtgc tctacaaggg aaccgatgtc
    29701 catgcattaa acggaaaaga gcggtttgcc ttcaaaaaac aggtgcagat ggtcttccag
    29761 gatccgtatg gatcgctcga cccgcgtatg accgttgccg acatcatcgg cgagggcatc
    29821 gacattcacc atctggcaaa gaataagaaa gagcgccagg agatgatcta caaatatctg
    29881 gagctggtcg gactgaaccg cgagcatgcg aaccgtttcg tgcacgagtt ttccggcgga
    29941 cagcgccagc gaatcggtat cgcgcgtgcg cttgccgtgc agccggagtt catcgttctc
    30001 gatgagccga tctccgcact cgacgtttcg attcaggcgc agatcgtcaa cctgcttgtg
    30061 gacttacaga agaagatggg tctgacctat cttttcgttg cgcatgatct gtcgatggtc
    30121 aaacatattt ccgaccgtgt ggcggttctg tatctgggaa cgctcgtcga gctgacgaca
    30181 tccgaggagc tgtacgccaa cccgcgccac ccgtacacac aggcactgct ctccgcgatt
    30241 ccgattccgg acccgaaagt agaggcagag cgcgaccgca aaaaaatccg cctcgaaggc
    30301 gaggtgccga gcccgatcaa cacgaagccg ggctgcaaat tccagggaag atgcaagtac
    30361 gcaaaagaca tctgccgaca gcagatgccg gaattaaaag acgtgggcaa caatcacttt
    30421 gtggcatgtc atttctgcga tgcgttagaa gaaaataagg aattataaaa catggaaaag
    30481 ataaagaaca tctgccgtga tctcggcaaa gcatttacaa tcggcctgct ctccggcggc
    30541 gctgcggcag ctgtgctgct gctgggagga ttcctctttg ggggattcac gttccgcgct
    30601 gcccttgagg cggcgaaaga cggcctgctg ctgatcgcgg ccctcggcat gttcgtgatc
    30661 gccggaatgc tgatggtaaa aggcaaaaac ccggagaaat tcacgcagaa ggaaaaatgg
    30721 cgcgtccact ttgccgtgct cggatataag agtgtgttcg cagttctctg cgtttcctgg
    30781 gtgctgtggg cggcagcggc ggattatctt ttgtatcgga tgtgaaaata gtttctataa
    30841 ataaaaagag aattttgcag actgtaaaaa tgaggtccgg caaaattctc ttttttcgtt
    30901 tagtcgttat tcgtcttcga gacgggagat aaccatctcc atggatttct gatattcttc
    30961 atccgaagaa ttgtagaaca gcatcagatc gaggcagctg ctcattttct tcagtgcttc
    31021 cgggatggag tagtcgatcg ttttcacata ttcatcccag tgctccagct tccacgtatc
    31081 gcgcacggag ttgcggtcga tcatgcgctg gtggcagatc tccggttttg tgacaaccca
    31141 gatcacaaca agctttgccc catggtcagc gagctttttg gagaagtctt tcaggaactt
    31201 ctcatcatgc acttcgcgtg tgaatggtgc gttgatcagc acaatatcgt catactcgag
    31261 tgcttccagt ccgaaattga ccgttacttc atattcataa tcgcggatat tctcctcgaa
    31321 gaaatcagaa ctgcggttga tttcctgtcc gcccgctttg aaaacgcggt tcgacagcgg
    31381 aatgaccgta tccttgtcga gataaacaac gtgtttcagc tccttggcga gctgttttga
    31441 aatgtatgtc tttccgcacg ccggcggcga ggttacgagg atcatctttt tcatcgtttg
    31501 tatgtcttcc tttccagttg aataaaaaaa atcactccta gcattataga aaatttggga
    31561 aaaaattgca agagaaaaga cggttgtgcc ggagaaaaaa ttgtcttata ataaaataag
    31621 atttttatgc catcacggag aaaataaatg gaggaaagac aatgagcaga gcaaatacac
    31681 gagttgtgaa aatcggcgac cgggtcatcg gaggcggaaa tccgattctg atccagtcca
    31741 tgacgaatac caaaacagaa gatgtggagg caaccgtaaa gcagatccag gcgctcacgg
    31801 aagccggatg tgacattatc cgatgcgccg tcccgaccat ggaggcagcc aaggcgctgt
    31861 ccgagatcaa aaagcagatc tcgattccgc ttgtagcaga tattcatttt gactatcgat
    31921 tggcgatcgc cgcgatcgaa aacggagccg ataaaatccg catcaacccg ggcaacatcg
    31981 gcagccgcga ccggatccag gcggttgtag atgcagcaaa agaacgccag attccgatcc
    32041 gcgtcggcgt caacagcggt tcactcgaaa aagagctggt ggaaaaatac aacggcgtaa
    32101 cggcagaggg cctggtggag agcgcgctcg acaaagtgcg gatcatcgag gagatgggct
    32161 atgacaatct cgtcatcagc atcaaatcgt ctgatgtgat gatgtgtgca aaagcgcatg
    32221 agctgatcac gaaccagacg gatcatccgc ttcatgtcgg cattacggag gcaggaacgc
    32281 tgtattccgg caacatcaaa tcggcagtgg ggctcggtat tattttatat cagggcatcg
    32341 gagatacgat ccgtgtttcc ctgaccggcg atccgctcga ggaaatcaaa tccgcaaaac
    32401 ggatcttaaa gaccctggga cttcgaaaag gcggcattga ggtcgtatcg tgtccgacct
    32461 gcggacggac ccagattgac ctgatcggac tggcaaacca ggtggaaaca ctggtgcagg
    32521 gctacgatct tgatatcaag gttgccgtca tgggctgcgt ggtcaacgga ccgggcgagg
    32581 caaaagaggc ggatcttggt gttgcaggcg gaaaaggcgt cggcatttta attaaaaaag
    32641 gtgaaatcgt gaaaaaagta ccggagagcg agctgctgtc cgtcttaaag gacgagctgg
    32701 atcactgggg caagtaaaaa agaaagcagg aataggaatg gaaaaacagt ttcaggaagt
    32761 atttccggat ttgagaatcg aagggggcat ggcagagctt ctcgataccg tggaggtttc
    32821 gcgggtatcg atcaataaga aaaaagatct gctgcggatc tatgtggtca gccatcagtg
    32881 gattcacaaa aaatatattt atcagctgga gcgccagatc gaggagcagc tcttttccaa
    32941 cgtgccgctg cgcgtgaaga ttattgagcg gttctatctg tcgcgccagt atacgccgga
    33001 aaactttctg gaggtgtacc gtccgagtat cctgctcgag ctgaaaaacc acagtgtctt
    33061 tctgaatcat ctgttttcga ctgccgagat taccttcccg gagacgaaca aaatgcacct
    33121 ggtgctcgtg gattccgttg tggcaaagga gcgcgaggag gagctggtcc acattctcga
    33181 gaaaatcttc tgtgagcggt gcggcttcga cctgatcgtg gaaaccgaga tgagaaagcc
    33241 ggagggcgag agccgcgcca tgagaaattc cgagctccgc atccgggagg aagtgcgcca
    33301 cgttctggcg catacccatt acggtgagat gctcggcgaa aaagagcaga accggatgga
    33361 agagcaggcg gaagcagcag gcgaacagcc aaaagcatcc gcagcagcca aggacggcga
    33421 ggcgaaaaaa gaaaaagcag cagacggcgg aaggggaaaa ggtttttccg gcggcggacg
    33481 cggttccttc aaaaaaggca agggcagttt cggccggaat gcggacaatc agccgcgcaa
    33541 atcctccaat ccggatgtgc tgtacggaag agatttcgag gatgagagcg tggagattct
    33601 gaaaatcggc gatgagatcg gtgatgttgt catccgagga agagtccaga gtgtggatat
    33661 gcgcgagatc cggaatgagc gtacgatttt catgttcacg atcaccgact ttacggatac
    33721 catcggcgtc aagattttcg tccagaacgc ggaagtgccg gaattaaagg atgcgatcaa
    33781 aaaaggcgcg ttcattaaag taaaaggaaa aacgacggtt gatgcatttg accacgatct
    33841 gacggttatg tctgtctggg gcatcaagaa gatcaccgat ttccgtaccg gccgtcagga
    33901 tacgagtccg gtgaagcgtg tggagctgca ctgccataca aagatgagcg atatggacgg
    33961 tgtcaccgat gctgcccgtc tggtacagcg tgcctacgag tggggccatc cggcgattgc
    34021 aatcaccgac cacggcgttg tgcagtcctt cccagaggca aaccatgcga tcgaggcgat
    34081 cgacggcgct taccggaaaa aataccaggc agagcacccg gatgcgacaa aagatgagct
    34141 gaaaaaagtt tcggctccgt tcaaggtcat ctacggcatg gaggcttatc tcgtcgacga
    34201 cttgaaagat atcgttgtca acagcaaagg acaggacatc cacggaagct acgttgtttt
    34261 cgatatcgag acgacgggat tttccccggt tgtcaacaaa atcattgaga tcggtgccgt
    34321 ccgtgtggaa aacggagcga ttgtcgataa gttttccacc ttcgttaacc cgaaagtccc
    34381 gatcccgttc cggattgaga acctgaccgg catcaacgac aacatggttc ttgacgcgcc
    34441 ggacatcgaa acggtgctgc cgaaattcct ggagttcagt gagggagccg ttatggttgc
    34501 ccacaatgct tcgttcgata tgagctttat cgagcacaac tgcgtcctgc aggggatcga
    34561 gcgggaattt acaacggcgg acaccgtcgc catggcgcgt ttcctgctgc cgggcctgaa
    34621 ccgtttcaag ctggataccg ttgcgaaggc ggtcggcgta tcgttggaaa accatcaccg
    34681 tgcggtggac gatgccggct gtacggcgga aattttcgtg aaattcgtaa aaatgctgga
    34741 ggagcgcaat attctgactc tggatgacct gaatgcgcag ggaaaagtat cggaggaggc
    34801 tgtgcgcaag cttccatcgt accatgcgat cattctggca aagaacgaga cgggacgtgt
    34861 caatctgtac cgcctcgtgt cggaatccca cctgaaatac tacaaccgcc ggccgaagct
    34921 gccaaagagc gtctacctga aataccagga cggtctgatg atcggatctg cctgcgaggc
    34981 gggcgaactc taccaggcaa tcctgcgcgg tgaaccggat gcggcgattg cgcgtctcgt
    35041 ggacttctac gattatctgg aaatccagcc gatcggcaac aacgcgttca tgatcaaaaa
    35101 agaggacgtt tctgccgtag agagcgagga ggatttaaag gagatcaaca ggcgcatcgt
    35161 caaacttggc gagcagttcc ataagcctgt tgtggcaacg tgcgacgtcc acttcatgga
    35221 tccgcaggac gagatctacc gccgcatcat catgaccgga aaaggttttg acgacgcgga
    35281 tgagcaggca ccgctgttcc tgcgcaccac cgaggagatg cttgaagagt tttcgtatct
    35341 cggaagtgag aaagcggaag aggtcgtcat caccaatccg cgcaaaatct ccgaccttgt
    35401 cgagaaaatc tccccgatcc gtgccggaaa attcccgccg gtcatcgagg attccgacaa
    35461 gacactgcgg agaatctgtt acgaccgcgc gcatgaaatc tacggcgagg agctgccgga
    35521 gatcgtttcc gcgcgcctcg aacgggagct gaactccatt atttccaacg gatatgccgt
    35581 tatgtacatc atcgcacaga aactagtgtg gaagtcgaac gaggacggct acctcgtagg
    35641 atcgcgtggt tccgtcggtt cctcgttcgt tgcgacgatg gcgggaatca cggaggtaaa
    35701 cccgttgagc ccgcattact actgcgagtc gtgccattac agcgagttcg attcgccgcg
    35761 cgtcaaagag tacgtcggcc gtgccggatg cgatatgccg gacgccgtct gcccgaactg
    35821 cggcaagccg ctgaaaaaag ccggatttga cattccgttc gagacgttcc ttggattcaa
    35881 gggaaacaaa gagccggata tcgacctgaa cttctccggt gattaccaga gtaaggcgca
    35941 caaatacaca gaggttattt tcggcgccgg tcagacgttc cgtgcgggaa ccatcggtac
    36001 gctggcggat aaaacagcgt tcggttacgt gaagaactat tacgaagagc gcggccagca
    36061 caaacggaac tgcgagatcg accggatcgt ggaaggatgc accggcatcc gccgtaccac
    36121 cggccagcac ccgggcggta tcatcgttct gccgttcggt gaggacatca actccttcac
    36181 cccggtgcag catccggcga acgatatgac aaccgacatt attacgaccc atttcgatta
    36241 ccactccatt gatgccaacc tcttaaagct cgacattctc ggacacgatg atccgaccat
    36301 gatccgtatg ctcgaggata tcacgcacct tgatgcgcag acgattccgc tcgataaccc
    36361 ggaggtcatg tcgctgttca agagcaccga ggcgctcggc atcaagccgg aggacatcgg
    36421 cggctgtccg cttggatgtc ttggcgtgcc ggagttcggt accgatttcg ttatccagat
    36481 gcttctggac acgaagccgc agtcgttctc cgatctgatc cgtatctcag gtctgtcgca
    36541 cggaaccgac gtatggctcg gcaatgcgca gaccctgatc gaggagggca aggcgacgat
    36601 ttccaccgcc atctgtaccc gggacgatat catgatctac ctgatcgaca agggactcga
    36661 aagtgagctt tcctttacga tcatggagag tgtgcgaaaa ggaaagggct taaaaccgga
    36721 gtgggaagag gagatgaagg cacatgacgt gccggactgg tacatctggt catgtaaaaa
    36781 gatcaagtac atgttcccga aagcccacgc ggcggcgtac gtcatgatgg cgtaccgcat
    36841 cgcttactat aaaattttct atccgctggc gtactatgcg gcgtacttct ccattcgtgc
    36901 gtctgcattc tcctacgagc tcatgtgcat gggacgcgac cggctggaat attatatgaa
    36961 agattacgaa aaacggaaag acacgctgac tcagaaggag caggcaaccg tcaaggatat
    37021 gaagattgtc caggaaatgt acgcccgcgg cttcgatttc gtgccggtgg acctctataa
    37081 ggcacaggcg caccgcttcc aggtctacga tgacaaacat ctgatgccgg cgctcgactc
    37141 gatcgagggt ctgggcgaca aggcggccga cgccgttgtc cttgcggcac gaaacggaaa
    37201 attcctctcg aaagatgatt tccgcgaccg caccaaggtc agcaagacga ttatcgactt
    37261 tatggcg
//
LOCUS       DAOMUF010000005        33432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_142_length_33432_cov_4.561345, whole genome shotgun sequence.
ACCESSION   DAOMUF010000005 DAOMUF010000000
VERSION     DAOMUF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 33432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33432
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142_length_33432_cov_4.561345"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLN77_00815"
     CDS             <1..444
                     /locus_tag="PLN77_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM42852.1"
                     /translation="TDNVPGIAGLAKSAEIVYRHFDEQTAQMRACKHRLIEGLRELDD
                     VVIHGMPEEEGAPQIVNASFLNVRSEVLLHTLEDRQIYVSAGSACSSHKRAGSPTLTA
                     IGASKAEMESAVRFSFSEFTTLEQIDYTLDTLREVLPMLRRFTRK"
     gene            461..1117
                     /gene="thyX"
                     /locus_tag="PLN77_00820"
     CDS             461..1117
                     /gene="thyX"
                     /locus_tag="PLN77_00820"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRM42853.1"
                     /translation="MITIQPEIEILSTESYEDMLRRIEKIGRVCYKSEERIGEGTAEK
                     FIAGIIKRGHESVIEHGSITVKVTCDRGVTHEIVRHRIASYSQESTRYCNYSKDKFGN
                     QITCIDLATGFSYDLDDENDRKKYEVWQKAMEAAEQYYFQMLELGAKPEEARSILPNS
                     LKTEIVMTMNLREWRHFIRLRSANAAHPQMRQVSDLILKKFSEEYPLFFRDLAEKKGE
                     "
     gene            1127..2305
                     /gene="thiI"
                     /locus_tag="PLN77_00825"
     CDS             1127..2305
                     /gene="thiI"
                     /locus_tag="PLN77_00825"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HRM42854.1"
                     /translation="MYKAFLVKYAEIGIKGKNRHLFEKALCVQMEHALAPVDGNFQVT
                     REMGRIYVNTEGDFDYEGAVDALTRVFGIAEICPVVILEDTGFEDLAEAVVSYVKELY
                     PTEEKTFKVCARRANKNYPMHTMEVNCELGGRILDVCPNLKVDVHEPDIYLNVELRQK
                     IYLYCESIAGPGGMPVGTNGQAMLLLSGGIDSPVAGYMIAKRGVKIDAVYFHAPPYTS
                     ERAKQKVVDLAKLVARYSGPIRLHVVNFTEIQLAIYEKCPHDELTIIMRRYMMKIAEA
                     FARQDKCLGLITGESIGQVASQTMHSLAVTNEVCTMPVFRPLIGFDKQEIVDISEKIG
                     TYETSVLPYEDCCTIFVAKHPVTKPNLNVIKCSERHLDDVIDEMMERAVSTAETIEVC
                     "
     gene            complement(2439..2672)
                     /locus_tag="PLN77_00830"
     CDS             complement(2439..2672)
                     /locus_tag="PLN77_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM42855.1"
                     /translation="MKTVQISLNSIDKVKSFVNDISKFDFDFDLVSGRYVIDAKSIMG
                     IFSLDLSKPIDLNVHADGDALQTVLDVLAPYIV"
     gene            2835..>3492
                     /locus_tag="PLN77_00835"
     CDS             2835..>3492
                     /locus_tag="PLN77_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM42856.1"
                     /translation="MKKTVALHNLGCKVNAYELEAIQQLLEDRGYEIVPFAPGADVYI
                     INTCTVTNIADRKSRQMLHKAKKMNPDAVVVACGCYVQAAGEKLEEDTAIDLIIGNNK
                     KKDLVDILENFLAEREDGGKPSDAECKSFLVDMTHNREYETLSISKTAEHTRAFLKVQ
                     DGCNQFCTYCIIPYARGRVRSRRQSDVVEEVKRLVEAGYQEVVLTGIHLSSYGLDFPE
                     G"
     assembly_gap    3493..3510
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3511..4168
                     /locus_tag="PLN77_00840"
     CDS             <3511..4168
                     /locus_tag="PLN77_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM42857.1"
                     /translation="LIRAVDAVDGIRRIRLGSLEPGIITEEFAEAIAAMPKVCPHFHL
                     SLQSGSNGTLKRMNRKYTAEEYKHKCDLLRKVYGNPALTTDVIVGFPQESEEEFEESR
                     AFVEDIHFFETHIFKYSRREGTRAAAMAGQIPEQEKTKRSHVLLELDERRRQEYMEGF
                     LGKELEVLMEEEVEIDGVHYWTGHTREYLRVAVLSEENLMNQMVLVTPARIHGKDCLM
                     "
     gene            4405..5340
                     /gene="murB"
                     /locus_tag="PLN77_00845"
     CDS             4405..5340
                     /gene="murB"
                     /locus_tag="PLN77_00845"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRM42858.1"
                     /translation="MRQRVKIVIKEEIVRELYAIAGEDAVQTAEPMKNHTTFRVGGPA
                     DYFVAPHTEEEIRKIVALCEEKEIPWFVTGNGSNLVVSDEGYHGVILSVYKNFQGIEV
                     EGNRIRAKAGSMLVSISRAAREAGLSGMEFASGIPGTLGGGVRMNAGAYGGEMKDIVQ
                     NVTVLTREGEIRELEKEELGFGYRASVIKDRGYVVLGAELMLVPGDKEEILARMQELK
                     NKRVEKQPLEYPSAGSAFKRPEGYFAGKLVMDAGLSGYAVGGAKVSEKHCGFLINAGG
                     ATASDVMELIRQIQAKVKEQFGVQLEPEIQFLGEF"
     gene            5370..6251
                     /gene="rapZ"
                     /locus_tag="PLN77_00850"
     CDS             5370..6251
                     /gene="rapZ"
                     /locus_tag="PLN77_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRM42859.1"
                     /translation="MRFVIVTGMSGAGKSTALKMLEDAEYFCVDNLPIPLVEKFAQLT
                     ADGTSGEIQKVAVGIDIRSGQALPDLQNVLEWMSINGICYEILFLDASDDVLVKRYKE
                     TRRTHPLAGLGRVDEGIRKERKALEFLKKDADYILDTSQLLTRELKAELDKIFVQDQK
                     FKNLMITVLSFGFKYGIPADSDLVFDVRFLPNPYYVEGMRPLSGNDAPVRDYVMGFET
                     AQVFAEKLEDMIRFLIPNYISEGKHQLIISIGCTGGKHRSVTLANELYRRLSNAKDYG
                     IRIEHRDLEKDAIRKGI"
     gene            6262..7200
                     /gene="whiA"
                     /locus_tag="PLN77_00855"
     CDS             6262..7200
                     /gene="whiA"
                     /locus_tag="PLN77_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRM42860.1"
                     /translation="MSFSSEVKEELSRHLPTARHCQIAETAAILSLCGQISEKDGTIR
                     IQTENAYVARKYFTLLKKTFNIEAELAVKLNQKENTYLIRVNDREETRQILQAVKLHE
                     TDRQLAMPIPAVVVQRQCCKRAFLRGAFLASGSISDPNRFYHLEIVCPDAPRAEQVQE
                     LLKSFGIDARIVLRKNHHVVYMKEGAQIVEMLGIMEANIALMNLENVRILKEMRGSVN
                     RKVNCETANINKTVSAAIRQIEDIRYIEETTGFSSLPEPLEQMARLRLQYPEASLLEL
                     GKMLTPVVGKSGVNHRLRKLKEIAEDLRRKQGGEVL"
     gene            7197..7460
                     /locus_tag="PLN77_00860"
     CDS             7197..7460
                     /locus_tag="PLN77_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM42861.1"
                     /translation="MIKKPITIRLSSGLEARPVAMLVQVASQYESEIYVESGRKKVNA
                     KSIMGMMTLGLDNGEEVTVTANGKDESEAMDGVEKYLSSPFAE"
     gene            complement(7560..8315)
                     /locus_tag="PLN77_00865"
     CDS             complement(7560..8315)
                     /locus_tag="PLN77_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM42862.1"
                     /translation="MNTLIKTIEDLSLNAWPSHQIELYDGWILRFSYFYTHRTNSVEQ
                     IGPSTIPVEEKVQYCEEIYRRWGTPAIFKISPLVNPGFDAMLADRGYVVQHTTHVMTM
                     DLANFAKKNPAVAVTVSSFIPPKWIDSLFALKSTTNVMHRMVVPSMYRAIPKETICTS
                     IQDETGTIVATGLGIRDRDYVGVYAIHVHPAYRHRHYASSICRTILSEGIRLGATKAY
                     LQVVEGNTPAITLYESLGFSYLYTYWFRAKNVV"
     gene            8503..9561
                     /locus_tag="PLN77_00870"
     CDS             8503..9561
                     /locus_tag="PLN77_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42863.1"
                     /translation="MSKLLKVIVNTLLLLAILSAGALVIPPFAGITTLVSDGMGNTNI
                     SRGAVTYAKKTDTSSVATGDKILVDDNSGKYIYTIQSLDTAAGTASVKNVISGDTTEI
                     TVRATVSKVIVTIPVIGFLSMAAQSREGIMIIGLAILFLVILFILSEVWKRDEEEDEE
                     EEEEDEDEDDEKASEELRSRKKAALEEKKKREEQEMTEQLRAADQKTAEEEKQDEFEI
                     PDLEEEGTEQVSFHTVDLQNLVRGEETEEIPEAVEETEIVEEAAAEETAEEVTEETEE
                     TESAEPGTVEEIEEFEETIEEPEEEPEPEPSEPIELAMPVYTAEELLQKAHAAGDDPK
                     VIEDPATGITILDYSDIL"
     gene            10071..10388
                     /gene="rpsJ"
                     /locus_tag="PLN77_00875"
     CDS             10071..10388
                     /gene="rpsJ"
                     /locus_tag="PLN77_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRM42864.1"
                     /translation="MASQVMRITLKAYDHQLVDASAQKIIETVKKNGATVSGPVPLPT
                     KKEVVTILRAVHKYKDSREQFEQRTHKRLIDIIAPTQKTVDALARLEMPAGVYIDIKM
                     KNK"
     gene            10582..11217
                     /gene="rplC"
                     /locus_tag="PLN77_00880"
     CDS             10582..11217
                     /gene="rplC"
                     /locus_tag="PLN77_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRM42865.1"
                     /translation="MKKAILATKVGMTQIFDENGVLVPVTVLQAGPCVVTQVKTADND
                     GYSAIQVGFGEKKENRVNKPMKGQFAKAGVSCKRYLKEFRFEDAETYTLGQEIKADIF
                     GAGDKIDATATSKGKGFQGAIKRHGQSRGPMAHGSKYHRHAGSNGACSDPSKVFKGKR
                     MPGQMGGKKITVQNLEIVKVDVENNLLLVKGAVPGPKKSMVTIKETVKCGK"
     gene            11244..11864
                     /gene="rplD"
                     /locus_tag="PLN77_00885"
     CDS             11244..11864
                     /gene="rplD"
                     /locus_tag="PLN77_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRM42866.1"
                     /translation="MANVSVLNMEGKEVGSMELNDAVFGVEINEHLVHQAVVLQLANN
                     RQGTQKAKTRSEVSGGGRKPWRQKGTGHARQGSTRAPQWTGGGVVFAPVPRDYSFKMN
                     KKEKRAALKSVLTSKVQENKFIVLDELKLAEVKTKEMKKVLDNLKVNNALVIIGDDSE
                     NVALSARNIAGVQTASVNTINVFDMLKYNTIIATKSSVASIEEVYA"
     gene            11864..12163
                     /gene="rplW"
                     /locus_tag="PLN77_00890"
     CDS             11864..12163
                     /gene="rplW"
                     /locus_tag="PLN77_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRM42867.1"
                     /translation="MADIRYYDVILKPIVTEKSMNAMGEKKYTFMVHPEANKSQIKEA
                     VEKMFEGTKVKSVNTMNMDGKNKRRGMVVGKTAKSKKAIVALTEDSKDIEIFEGL"
     gene            12225..13070
                     /gene="rplB"
                     /locus_tag="PLN77_00895"
     CDS             12225..13070
                     /gene="rplB"
                     /locus_tag="PLN77_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRM42868.1"
                     /translation="MGIKSFNPYTPSRRHMTSSDFNEITKSTPEKSLVVSLKKNAGRN
                     NQGKITVRHRGGGSRRKYRIIDFKRRKDDVPAIVKSIEYDPNRTANIALICYADGEKA
                     YILAPEGLKVGMKVMSGASAEVRPGNCLPLSEIPVGTMVHNVELHAGKGGQLVRAAGN
                     GAQLMAKEGKYATLRLPSGEMRMVPINCRASIGVVGNGEHSLINIGKAGRKRNMGIRP
                     TVRGSVMNPNDHPHGGGEGKTGIGRPGPSTPWGKPALGLKTRAKNKQSNKYIVRRRDG
                     KALSK"
     gene            13082..13363
                     /gene="rpsS"
                     /locus_tag="PLN77_00900"
     CDS             13082..13363
                     /gene="rpsS"
                     /locus_tag="PLN77_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRM42869.1"
                     /translation="MSRSLKKGPFADESLLKKVDAANASGDKTVIKTWSRRSTIFPSF
                     VGHTIAVHDGRKHVPVYVTEDMVGHKLGEFVATRTYRGHGKDEKKSGVR"
     gene            13397..13783
                     /gene="rplV"
                     /locus_tag="PLN77_00905"
     CDS             13397..13783
                     /gene="rplV"
                     /locus_tag="PLN77_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRM42870.1"
                     /translation="MAKGHRSQIKRARNANKDTRPSAKLSYARMSAQKAGFVLDAIRG
                     KDVTTALGIVTYNPRYASSVVKKLLESAIANAENNNGLSRENLYVAECFANKAPTMKR
                     IHPRAQGRAYRILKRNSHITIVLDEK"
     gene            13796..14452
                     /gene="rpsC"
                     /locus_tag="PLN77_00910"
     CDS             13796..14452
                     /gene="rpsC"
                     /locus_tag="PLN77_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRM42871.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSKWYAEGDFADNLVEDYNIRTYLKK
                     KLYSAGVSKIEIERASDRVKVIIYTAKPGVVIGKGGAEIEKVKAEVQKLTDKKLIVDI
                     KEVKRPDRDAQLVAENIALQLENRISFRRAMKSTMQRTMRAGAKGIKTSVSGRLGGAD
                     MARTEFYSEGTIPLQTLRADIDYGFAEADTTYGKVGVKAWIYNGEVLPTRTKKEGSEN
                     "
     gene            14455..14892
                     /gene="rplP"
                     /locus_tag="PLN77_00915"
     CDS             14455..14892
                     /gene="rplP"
                     /locus_tag="PLN77_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRM42872.1"
                     /translation="MLMPKRVKRRKQFRGSMKGKALRGNKINYGEFGLVALEPCWIKS
                     NQIEAARIAMTRYIKRGGKVWIKIFPDKPVTAQPAETRMGSGKGSLEYWVAVVKPGRV
                     MFEIAGVPEETAREALRLAMHKLPCKCKVVSRADLEGGDNSEN"
     gene            14882..15091
                     /gene="rpmC"
                     /locus_tag="PLN77_00920"
     CDS             14882..15091
                     /gene="rpmC"
                     /locus_tag="PLN77_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRM42873.1"
                     /translation="MKTNEFVKDLNTKSAAELNDELVAAKKELFNLRFQNATNQLDNT
                     SRIKEVRKNIARIQTVIAQKANAAE"
     gene            15108..15365
                     /gene="rpsQ"
                     /locus_tag="PLN77_00925"
     CDS             15108..15365
                     /gene="rpsQ"
                     /locus_tag="PLN77_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRM42874.1"
                     /translation="MEERNLRKTRVGKVVSDKMDKTIVVAIEDHVKHPLYKKIVKSTY
                     KLKAHDANNECHVGDTVRVMETRPLSKDKRWRLVEIVEKAK"
     gene            15401..15769
                     /gene="rplN"
                     /locus_tag="PLN77_00930"
     CDS             15401..15769
                     /gene="rplN"
                     /locus_tag="PLN77_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRM42875.1"
                     /translation="MIQQETRLKVADNTGAKEILCIRVMGGSTRRYANIGDVIVASVK
                     DATPGGVVKKGDVVKAVVVRSVKGARRKDGSYIKFDENAAVIIKDDKTPKGTRIFGPV
                     ARELREKQFMKIVSLAPEVL"
     gene            15780..16121
                     /gene="rplX"
                     /locus_tag="PLN77_00935"
     CDS             15780..16121
                     /gene="rplX"
                     /locus_tag="PLN77_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRM42876.1"
                     /translation="MSMKIKKGDTVKVIAGKDKDKTGKVVSVDVKNNRVVVEGVNMIT
                     KHEKPSMSNQQGGIVNKEAPIDASNVMLVVKGVPTRVGIKVEMVEKNGKTKAVRTRVA
                     KAKELNGAVID"
     gene            16142..16681
                     /gene="rplE"
                     /locus_tag="PLN77_00940"
     CDS             16142..16681
                     /gene="rplE"
                     /locus_tag="PLN77_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRM42877.1"
                     /translation="MSRLKDLYNEQIVDAMTKKFGYKNVMEVPKLSKIVINMGVGEAK
                     ENAKLLDAAISDMEAITGQKAVTTKAKNSVANFKIREGMPIGCKVTLRGEKMYEFADR
                     LINLALPRVRDFRGVNPNAFDGRGNYALGIKEQLIFPEIEYDKVDKVRGMDIIFVTTA
                     NTDEEARELLTLFNMPFAK"
     gene            16698..16883
                     /locus_tag="PLN77_00945"
     CDS             16698..16883
                     /locus_tag="PLN77_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRM42878.1"
                     /translation="MAKTAMKIKQQRKAKFSTREYNRCRICGRPHAYLRKYGICRICF
                     RELAYKGQIPGVKKASW"
     gene            16908..17309
                     /gene="rpsH"
                     /locus_tag="PLN77_00950"
     CDS             16908..17309
                     /gene="rpsH"
                     /locus_tag="PLN77_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRM42879.1"
                     /translation="MSMSDPIADMLTRIRNANTAKHDTVDVPASKMKIAIADILVNEG
                     YIAKYELSDEGAFQNMHITLKYGADKNEKIITGLKRISKPGLRIYVGKDELPRVLGGL
                     GIAILSTNQGVITDKEARKLQVGGEVLAFVW"
     gene            17391..17930
                     /gene="rplF"
                     /locus_tag="PLN77_00955"
     CDS             17391..17930
                     /gene="rplF"
                     /locus_tag="PLN77_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRM42880.1"
                     /translation="MSRIGRLPVAIPAGVTVDIQENNHVVVKGPKGTLERTLPAEMSI
                     KLEDGHVVVTRPSDLKKMKALHGLTRTLIHNMVVGVSEGYTKTLEVNGVGYRAAKQGN
                     KLVLSLGYSHPVEMIDPEGLSSAVDGNKIIVSGISKEKVGQYAAEIRDKRRPEPYKGK
                     GIKYADEVIRRKVGKTGKK"
     gene            17948..18316
                     /gene="rplR"
                     /locus_tag="PLN77_00960"
     CDS             17948..18316
                     /gene="rplR"
                     /locus_tag="PLN77_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRM42881.1"
                     /translation="MISKVSRAQMRQKKHRRLRNHIVGTAQRPRLCVFRSNNHMYAQI
                     IDDTIGNTLVSASTLQKDVKAELEKTNNVDAAAYLGTVLGKKAVEAGITEVVFDRGGF
                     IYQGKIQAFADAAREAGLVF"
     gene            18337..18846
                     /gene="rpsE"
                     /locus_tag="PLN77_00965"
     CDS             18337..18846
                     /gene="rpsE"
                     /locus_tag="PLN77_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRM42882.1"
                     /translation="MKHTIIDASQFELEEKVVSIKRVTKVVKGGRNFRFTALVVVGDG
                     NGHVGAGLGKAAEIPEAIRKGKEDAMKKLVSVAINENGSITHDYVGKFGSAEVLLKKA
                     PEGTGVIAGGPARAVIELAGIKNIRTKSLGSRNKQNVVLATIAGLSELKTPEEVARLR
                     GKSTNEIFA"
     gene            18861..19043
                     /gene="rpmD"
                     /locus_tag="PLN77_00970"
     CDS             18861..19043
                     /gene="rpmD"
                     /locus_tag="PLN77_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRM42883.1"
                     /translation="MADKLKVTLVKSPIGCVPKHRRTVEALGLKKVNKTVELPDNAAV
                     RGMVNQVSYLVKVEEA"
     gene            19056..19496
                     /gene="rplO"
                     /locus_tag="PLN77_00975"
     CDS             19056..19496
                     /gene="rplO"
                     /locus_tag="PLN77_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRM42884.1"
                     /translation="MDLSNLQPAEGSRQNDNFRRGRGHGSGNGKTAGKGHKGQKARSG
                     APRPGFEGGQMPLYRRLPKRGFKNRNSKEIVGINVDALNRYEDGAVVTVETMIETGLV
                     SNPRDGVKILGNGEVTKKLTVKANAFSESAKAKIEAAGGTVEVI"
     gene            19496..20812
                     /gene="secY"
                     /locus_tag="PLN77_00980"
     CDS             19496..20812
                     /gene="secY"
                     /locus_tag="PLN77_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRM42885.1"
                     /translation="MFKTFINAFKVKDVRRKIFYVLMMLVVIRLGSQMPVPGVNTSFF
                     ANYFANNTNEAFSFLNAFTGGGFDRFSIFALSITPYITSSIIIQLLTIAIPRLEEMQR
                     DGEEGRKKMTAITRYVTVGLALFESVAMAVGFGRQGLLTTFNAVNVIVVVAALTAGSA
                     FLMWVGEQINDKGVGNGISMVLLINILSRIPQDMVTLYETFVKGKTIARGLLMWIIIA
                     VVIIAVVVLVLILNGAERRIPVQYSKKMAGRKMVGGQSSNIPLKVNTAGVIPIIFASS
                     IMSFPSIILSFVGKSDIKGIGGTLIKMLNSNNWFDKTNPVASLGLILYIVLVIFFAYF
                     YTSITFNPMEVADNMKKQGGFIPGIRPGKSTVDYLNNLLSYIIFIGAAGLTIVAVIPF
                     FFNGFFKANVSFGGTSLIIIASVILETLKQIESMMLVRNYKGFLND"
     gene            20980..21624
                     /locus_tag="PLN77_00985"
     CDS             20980..21624
                     /locus_tag="PLN77_00985"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRM42886.1"
                     /translation="MKIIMLGAPGAGKGTQAKQIAAKYEIPHISTGDIFRANIKEGTE
                     LGKKAKAFMDKGELVPDELTCDLVVDRISKPDAAKGYVLDGFPRTIPQAEALTKALEA
                     RGEKIDYAINVEVPDENIVHRMGGRRACIGCGATYHVEFNAPKVEDVCDTCGGELVLR
                     DDDKPETVQKRLSVYHAQTQPLIDYYKKANVLVEVDGTQDINVVFQDIVKILGA"
     gene            21628..22398
                     /gene="map"
                     /locus_tag="PLN77_00990"
     CDS             21628..22398
                     /gene="map"
                     /locus_tag="PLN77_00990"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRM42887.1"
                     /translation="MSISIKTAREIEKMRRAGELLEKVHDELGRAIHPGMSTWEINKL
                     GEEMIRSFGCTPNFLNYNGYPASICVSVNEEVVHGIPSKKRILKEGDIVSLDAGLIYE
                     GYHSDAARTHAVGEISPEARKLVEVTRQAFFEGIKYAKAGHHLHEISAAIGAYAESFG
                     YGVVRDLVGHGIGTHLHEDPAVPNFAQPSRGVRLQPGMTLAIEPMITAGGYAVRWLDD
                     DWTVVTEDGSLAAHYENTILITEGEPEILTLSHTEEDL"
     gene            22395..22646
                     /locus_tag="PLN77_00995"
     CDS             22395..22646
                     /locus_tag="PLN77_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HRM42888.1"
                     /translation="MKPIVKGMFARSLAGHDKGRLYVISDVQEPYVFLVDGKIRTANR
                     PKKKKLIHVQPDYEMTDAVRDEDIRKALKEKSCKELKED"
     gene            22650..22868
                     /gene="infA"
                     /locus_tag="PLN77_01000"
     CDS             22650..22868
                     /gene="infA"
                     /locus_tag="PLN77_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRM42889.1"
                     /translation="MSKADVIEVEGTVLEKLPNAMFKVELENKHVVLAHISGKLRMNF
                     IRILPGDKVTIELSPYDLDKGRIIWRDK"
     gene            23553..25025
                     /locus_tag="PLN77_01005"
     CDS             23553..25025
                     /locus_tag="PLN77_01005"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRM42890.1"
                     /translation="MTKMGKKKKKGLVAALSVVAVIVLLAAAYGIFCLIIEDDKIWAD
                     ASINGVNIQGMSKKEAAQTVEQKFEEDYKDTAVTVELDGQQYTMNVFPMLGMDASAEI
                     EKAYEKGHGNLLVRGLEWVEMKRGKAEKLSYDVQPTVAHPDEVEGIVQASGIQDYNSM
                     QDTTYEVTDTGLIVHKGIAGTRPDVDALKQQIQTNVTAMNFQDVISCPTAENQLTEPD
                     FAGIASQIYTEPVNATLDPDNGYSVVASKNGTSMDADSAKAEYESAEENTDITIPFTF
                     TEPEITTEKMNANLFKDTLGSYSSSAAGGTSGRIHNVGLTASICNGQIVLPGETFSFN
                     GVVGDTTAEKGYQEAAGYQDGKVVQVLGGGECQVSSTLFSAILYTDLDVLERANHSMP
                     VHYVPSGMDATVFWDSPDFKFENNHKYPVKIVMNMDSSDTLTVKILGTKENDYTIEPR
                     VEQIDEMSNVTYRDYKDASGNVVKSESVCASKYKPLNSGS"
     gene            25036..25329
                     /locus_tag="PLN77_01010"
     CDS             25036..25329
                     /locus_tag="PLN77_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42891.1"
                     /translation="MLPVKNRRDREEKSVPPVFFLTGGRDEKMHRKRAKKNEKICKKF
                     LAFPGTKCYTIKRTFARGLFSNPQKENAFQKFTTENSPTRGARSKERRIHHEG"
     gene            25319..25432
                     /gene="rpmJ"
                     /locus_tag="PLN77_01015"
     CDS             25319..25432
                     /gene="rpmJ"
                     /locus_tag="PLN77_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRM42892.1"
                     /translation="MKVRSSVKPICEKCKVIKRKGSIRIICENPKHKQRQG"
     gene            25728..26096
                     /gene="rpsM"
                     /locus_tag="PLN77_01020"
     CDS             25728..26096
                     /gene="rpsM"
                     /locus_tag="PLN77_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRM42893.1"
                     /translation="MARIAGVDLPRDKRVEIGLTYIYGIGRTSADRILKEAGVNPDTR
                     CRDLTDDEVKRLSAVIDESQVVEGDLRREIAMNIKRLQEIGCYRGIRHRKGLPVRGQK
                     TKTNARTRKGPKRTVANKKK"
     gene            26147..26539
                     /gene="rpsK"
                     /locus_tag="PLN77_01025"
     CDS             26147..26539
                     /gene="rpsK"
                     /locus_tag="PLN77_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRM42894.1"
                     /translation="MAKVTKKTTKRRVRKNVEHGQAHIQSSFNNTIVTLTDAEGNALS
                     WASAGGLGFRGSRKSTPYAAQMAAETATKAALVHGLKTVDVMVKGPGSGREAAIRALQ
                     ACGLEVTSIRDVTPVPHNGCRPPKRRRV"
     gene            26556..27149
                     /gene="rpsD"
                     /locus_tag="PLN77_01030"
     CDS             26556..27149
                     /gene="rpsD"
                     /locus_tag="PLN77_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRM42895.1"
                     /translation="MAVNRVPVLKRCRSLGMDPVYLGIDKKSTRQLKRANRKMSEYGL
                     QLREKQKAKFIYGVLEKPFVNYYKKADRMEGQTGENLMILLELRLDNVLFRMGMARTR
                     KEARQIVDHKHVLVNGKQVNIPSYLVSAGDTIEIKEGKKSSQRYKDIVEVTGGRLTPE
                     WIEADLEALKGVVKERPTREMIDVPVDEVQIVELYSK"
     gene            27222..28184
                     /locus_tag="PLN77_01035"
     CDS             27222..28184
                     /locus_tag="PLN77_01035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRM42896.1"
                     /translation="MFDFNKPKIEITEISDDKKYGRFVVEPLERGYGITLGNSLRRIM
                     LSSLPGAAVSQVKIEGVLHEFSSIPGVKEDVTEIIMNLKSLAIRNNSETNEAKVAYIE
                     FEGEGVVRASDIQVDQDIEIMNPDQVIATLNGGPDSKLYMELTITKGRGYISADKGKS
                     DDMPIGVLAVDAIYTPVERVNLKVENTRVGQITDYDKLTLDVYTRGTLDPDEAVSLAA
                     KVLSEHLKLFIDLSENAKTAEVMIEKEDNEKEKVLEMNIDELELSVRSYNCLKRAGIN
                     TVEELCNKTSEDMMKVRNLGRKSLEEVLAKLKELGLSLSPGDGE"
     gene            28289..28825
                     /locus_tag="PLN77_01040"
     CDS             28289..28825
                     /locus_tag="PLN77_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HRM42897.1"
                     /translation="MAKYRKLGRTSDQRKALLRNQVTNLLYHGKIVTTETKAKEIRKI
                     AESMIALAVKEKDNFETVTVTAKVARKDADGKRVKEVKDGKKVTVYDEVQKEIKKDLP
                     SRLHARRQMASFFYPVTEVPTEAAGRKKNTKSIDMPQKMFDEIAPKYADRNGGYTRII
                     KIGPRKGDAAMEVVIELV"
     gene            29012..29911
                     /locus_tag="PLN77_01045"
     CDS             29012..29911
                     /locus_tag="PLN77_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HRM42898.1"
                     /translation="MESKKKMLFIFNPFSGKAQIKSKLFEIIDVFVKGGYEVIVHPTQ
                     AVGDGFEKTKELAPQVDLVVCSGGDGTLDEVVSGLMEVDQRVPIGYIPAGSTNDFANS
                     LSISKDMVQAAKDIIEGNLYSCDVGAFNNDSFVYIAAFGLFTDVSYETDQHMKNILGH
                     LAYLLEGSKRIWNVPTYWIKVEANGETFEGEYIYGMVTNAKSVGGFKNLPGQDVRLDD
                     GLFEVTLIKRPKNPLELNEIIASLLMQEDHTDLIDSFKTDRIIIESNQEISWTLDGEY
                     GGDHGYVCIEARKHAMELVLNNK"
     gene            30091..30954
                     /gene="fba"
                     /locus_tag="PLN77_01050"
     CDS             30091..30954
                     /gene="fba"
                     /locus_tag="PLN77_01050"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HRM42899.1"
                     /translation="MLVSAKEMLDKAKAGHYAVGQFNINNLEWTKSILLTAQEMNSPV
                     ILGVSEGAGKYMTGFKTVAAMVKAMIEELNITVPVALHLDHGTYEGCYKCIEAGFSSI
                     MFDGSHYPIEENVAKTKELVAVCKEKGMSLEAEVGSIGGEEDGVVGMGECADPNECKM
                     IADLGVDMLAAGIGNIHGVYPANWQGLSFETLAAVQELTGTMPLVLHGGTGIPDDMIK
                     KAIDLGVSKINVNTECQLAFAAATRKYIEEGKDQQGKGFDPRKLLAPGAEAIKATVRE
                     KIELFGSANKA"
     gene            31369..>33432
                     /locus_tag="PLN77_01055"
     CDS             31369..>33432
                     /locus_tag="PLN77_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRM42900.1"
                     /translation="MGEYVNPAKIFGENVFNDTVMQERLPKNVYKKLKKIIEEGGELD
                     LATADTIAHEMKEWAIEKGATHYTHWFQPLTGTTAEKHDSFITAPMPNGKVLMSFSGK
                     ELIKGEPDASSFPSGGLRATFEARGYTAWDCTSPAFVRQDAAGATLCIPTAFCSYTGE
                     ALDQKTPLLRSMEALNVQSLRLLKLFGNTTSKKVTPSVGPEQEYFLVDAEKFLQRKDL
                     IYTGRTLFGAMPPKGQEMDDHYFGTIRQRIASFMKEVNIELWKMGVTAKTQHNEVAPA
                     QHELASIYSEANVAVDNNQLVMQTLKRVACQHGLKCLLHEKPFAGVNGSGKHNNWSIT
                     TDDGINLLEPGKTPHENIQFLLVLSCVLKAVDVHADLLRESAADPGNDHRLGANEAPP
                     AIISVFLGEQLEDVVEQLISTGNATKSKKEGVLETGVKTLPDLKKDATDRNRTSPFAF
                     TGNKFEFRMVGSRDSVAAPNIVLNTIVAEAFRDACDVLEGAENFEDAVHDLIKKNLSE
                     HQRIIFNGDGYADEWLAEAERRGLPNIKSMVYAIPALTTDTAIKLFGDFKVFTEAELV
                     SRAEVKFENYAKTINIEAKTMIDMASKQIIPAVIKYATSLAGSINTITAAGVTAVGVQ
                     KNLLNETSALLEETQKALDELIAIENAGCEMEDGEAKAKYYYEKVAPAMEALRAPVDK
                     LEMIVD"
BASE COUNT        10454 a         7014 c         8576 g         7370 t
ORIGIN      
        1 accgacaatg tgccgggcat cgcaggcctt gcgaaatccg ctgagatcgt ttacaggcat
       61 tttgacgagc agactgcgca gatgcgtgcc tgcaaacacc gtctgatcga aggcttgcgg
      121 gagctggacg atgtggtcat ccatggcatg ccggaagaag agggtgcgcc gcagattgtc
      181 aatgcatcgt tcttaaatgt gcgtagtgaa gtgctgctgc acacgctgga ggaccgccag
      241 atctacgttt ccgccggaag cgcctgctca tcacacaaga gagccggaag cccgacgctg
      301 actgccatcg gcgcatcgaa agccgagatg gagtctgccg tgcggttcag cttcagcgaa
      361 tttacgacac tggaacagat tgattacacc ctggatacgc tgcgcgaggt actgccgatg
      421 ctgcgcagat ttaccaggaa ataatagaaa gaggaaaatt atgattacaa tacagccgga
      481 aattgaaatc ctgagcaccg aatcctacga ggatatgctg cgccggatcg agaaaatcgg
      541 aagagtctgc tacaagagcg aggagcgcat tggagaaggc actgcagaga aattcatcgc
      601 aggcatcatc aaacgcggtc atgaatccgt cattgagcac ggctccatca ccgtaaaagt
      661 aacgtgcgac cgcggcgtga cccatgagat cgtccgccac cgtatcgcaa gctacagcca
      721 ggagagcacc cgctactgca actattcgaa agataaattt ggaaaccaga tcacctgtat
      781 cgacctggca accggatttt cctatgatct ggacgacgag aacgaccgta aaaaatacga
      841 ggtatggcag aaagcaatgg aagctgcaga gcagtattac ttccagatgc tcgaactcgg
      901 agcaaagccg gaggaggcaa gaagcattct tccgaactcc ctgaaaaccg agatcgtcat
      961 gaccatgaac ttaagagagt ggcgccactt tatccggctt cgaagcgcca acgccgcaca
     1021 tccgcagatg cgtcaggtat ctgacctgat cctgaagaaa ttttctgagg aatatcctct
     1081 gttcttccgc gatctggctg agaaaaaagg agaataaaaa acgatgatgt acaaagcatt
     1141 tctggtaaaa tatgccgaga tcggcattaa aggaaagaac cgtcatctgt tcgagaaagc
     1201 gctctgcgta cagatggagc acgcgcttgc cccggtagac ggaaatttcc aggtcacccg
     1261 tgagatggga cgaatctatg tcaacaccga aggagacttt gattatgagg gcgctgttga
     1321 cgcgctgaca agagttttcg gtatcgccga gatctgcccg gttgtcattt tagaggacac
     1381 cggctttgaa gatctggcag aggcagtggt ttcctacgtg aaagagctgt acccgaccga
     1441 ggagaaaacc tttaaggtat gcgcacgccg tgcgaacaaa aactatccga tgcacacaat
     1501 ggaagtcaac tgcgagctgg gcggccgcat cctcgatgtc tgcccgaacc tcaaagtaga
     1561 cgttcacgag ccggatatct atctgaacgt cgagctgcgt cagaagattt atctgtactg
     1621 cgagagcatc gcaggccctg gcggtatgcc ggtcggcaca aacggacagg ccatgctgct
     1681 gctttccggc ggtatcgaca gcccggttgc cggatacatg atcgcaaaac gcggtgttaa
     1741 gattgatgcc gtttacttcc acgctccgcc gtacaccagc gagagagcca aacagaaggt
     1801 tgtggatctt gcaaaactcg ttgcacgcta cagcggcccg atccgtctgc atgtggtcaa
     1861 cttcaccgag atccagcttg caatctacga aaaatgcccg cacgacgagc tgaccatcat
     1921 catgcgccgt tatatgatga agatcgcaga agcgtttgca cgtcaggaca aatgccttgg
     1981 cctgatcacc ggagagagta tcggacaggt ggcaagccag acgatgcatt ccctcgccgt
     2041 taccaacgaa gtctgcacca tgccggtctt ccgtccgctg atcggtttcg acaaacagga
     2101 gatcgtcgat atttccgaaa aaatcggtac gtacgagaca tctgttctgc cgtatgagga
     2161 ctgctgcacg atcttcgttg caaagcatcc ggtgacaaaa ccgaacctga atgtcatcaa
     2221 atgctccgag cgccatctgg acgacgtgat cgatgagatg atggaacgcg cagtttctac
     2281 cgcagagacg atcgaggtct gctaaggaaa acttgcttta aagaacaaaa aggccggatc
     2341 ttgttttggg ggttctgaaa aacaagatcc ggtcctgatg ataaacaaag acaaaaaaag
     2401 agccgcggcg agtgcgcggc ccttatgttc tatcgtaatt atacaatgta cggagcaaga
     2461 acatcaagta cggtctgaag agcatctcca tcagcatgga cgttcaggtc gatcggtttg
     2521 gaaaggtcga ggctgaagat tcccatgatg gattttgcgt cgattacgta tcttccggat
     2581 accagatcga aatcaaaatc gaatttggaa atgtcgttga cgaaagattt tactttgtcg
     2641 atagaattaa gagaaatctg tactgttttc atgtttgtaa tcctccctgt tatttggtta
     2701 acattattta ttaccctcat ttaacagctt tatcttaccg tctggagggg aaaaagtcaa
     2761 tgcgaaaaca tacaaagata aagtggaatc ttcgtgattt ttaataataa agatgaagaa
     2821 gaaaggagta tggcatgaaa aaaacggtag ctttgcataa tcttgggtgt aaagtgaacg
     2881 catatgagct ggaagccatc cagcagctcc tcgaagacag agggtacgag atcgtgcctt
     2941 ttgcaccggg agcggatgtt tacattatca atacgtgtac cgtgacgaac attgcggacc
     3001 ggaaatcgcg gcagatgctg cacaaagcga aaaagatgaa tcccgatgcg gttgtcgtgg
     3061 cctgcggctg ttatgtgcag gcggctgggg aaaaactcga agaggatacg gcgattgatc
     3121 tgatcatcgg aaataataag aagaaagatc ttgtggatat tctggaaaac tttctggcag
     3181 aacgtgagga tggtggaaaa ccatccgacg cggagtgtaa gagcttcctc gtggatatga
     3241 cacacaaccg tgagtacgag acactctcga tcagcaaaac ggcggagcac acccgcgcat
     3301 tcctgaaagt acaggatgga tgcaatcagt tctgtactta ttgtattatc ccgtacgcgc
     3361 gcggcagagt cagaagccgc cgccagagtg atgtggtaga agaagtaaaa cgtctggtgg
     3421 aggctggata ccaggaagtc gtgctgaccg gtatccacct ttcctcctac ggactcgatt
     3481 tcccggaggg cgnnnnnnnn nnnnnnnnnn cctgatccgc gctgttgatg cagtggacgg
     3541 catcagaaga atccgtctcg gatccctgga gccgggcatc atcaccgaag agtttgcaga
     3601 ggcgatcgcc gcaatgccga aggtgtgccc gcatttccat ctttcgctgc agagcggaag
     3661 caatggaaca ctgaagcgga tgaaccggaa atataccgca gaagaataca aacacaaatg
     3721 cgatcttctg cgtaaggtat atggaaatcc ggcgctgacg accgatgtga ttgtcggttt
     3781 cccgcaggag agtgaggagg agtttgaaga gtcgcgcgcc tttgtcgagg acattcattt
     3841 cttcgagact cacatcttca agtattcgag aagagaaggc acccgcgccg cagcgatggc
     3901 aggccagatt ccggagcagg agaagacgaa gcgcagccac gttctgctgg aactggacga
     3961 gagacggaga caggaatata tggaaggctt cctcggaaaa gagctggaag tgctcatgga
     4021 agaagaagtc gagatcgacg gcgtccatta ctggacggga cacacgagag aatatctgcg
     4081 cgttgctgtt ctttcagaag aaaatctcat gaatcagatg gtgcttgtca ccccggcgag
     4141 aatccatgga aaagactgtc tgatgtagga aatgatacca atccggaaga tctgatagaa
     4201 aaaagagccg gaacgaaaaa gttcgttgca ctttttgagg gttcgtggta gaataccccg
     4261 tgagcctgca aaaaggcggg cagacgaaga agaaaaacag cggctggcag aacgaacgaa
     4321 gaagtgaggg atgtcggctg ctttcgcgtc taaaaagtat aatctgtaat tcgtagtaat
     4381 tcgtaatgaa atgttcggcc gcagatgcgg caaagggtaa aaatcgtgat caaagaagag
     4441 attgtcagag aactttatgc aattgcaggg gaagatgcgg tacagaccgc agaaccgatg
     4501 aaaaatcata cgaccttccg tgtgggcggt ccggcagatt atttcgtcgc tcctcataca
     4561 gaagaagaaa ttagaaaaat cgtggctctc tgcgaagaaa aagagatccc gtggttcgtc
     4621 accggaaacg gcagcaatct cgttgtaagt gacgagggct accacggtgt gattctttcg
     4681 gtttacaaaa actttcaggg aatcgaagtg gaaggaaaca gaatccgggc gaaagccgga
     4741 agcatgcttg tatccatcag ccgggcggca agagaagccg gtctttccgg tatggagttt
     4801 gcctccggaa tccctggaac tttaggcggc ggcgtccgta tgaacgcggg agcctacggc
     4861 ggagagatga aggatattgt acagaatgtc actgtactca caagagaagg tgagatccgg
     4921 gaactggaaa aagaagagct cggcttcggc taccgtgcca gcgtcatcaa agacagaggc
     4981 tacgtcgtac ttggagcaga acttatgctt gttccgggag ataaagaaga aattcttgcc
     5041 cgcatgcagg aattgaaaaa caagcgggta gagaaacagc cgctcgagta tccgagcgcc
     5101 ggcagcgcat tcaagcggcc ggaaggctat ttcgcaggca agcttgtgat ggatgccgga
     5161 ctttccggct acgcagtcgg cggtgcaaaa gtatcggaaa aacactgcgg cttcctgatc
     5221 aatgcaggcg gtgcaaccgc atcggatgtg atggaactga tccgtcagat ccaggcaaaa
     5281 gtaaaagaac agtttggcgt acagcttgag ccggaaatcc agttcctcgg ggaattctga
     5341 ttttcgttat ccaccaaagg agaaaaaaca tgcgttttgt aattgtgacc ggcatgtccg
     5401 gtgcgggaaa aagtacagcg ttgaaaatgc tggaggacgc ggaatatttc tgcgtcgata
     5461 accttccgat cccgctggtt gaaaaatttg cacagcttac cgccgacggt acttccggag
     5521 aaatccagaa agtggcagtc ggaatcgata tccgaagcgg ccaggcgctc ccggacctgc
     5581 agaatgttct ggagtggatg agcatcaacg gaatctgcta tgagatcctg tttctggacg
     5641 cgtccgatga cgtgctggtc aaacgataca aagagacgag aagaacgcac ccgctggcag
     5701 gtcttggacg ggtggatgag ggaattagaa aagagcgcaa agcacttgag ttcctgaaaa
     5761 aagatgcgga ctacattctc gatacgagcc agcttctcac aagagagctg aaagcggagc
     5821 tcgataagat tttcgtgcag gatcagaaat tcaaaaatct gatgattact gtgctctcat
     5881 ttggattcaa atacggaatc ccggcggatt cggaccttgt tttcgatgtg cggttcctgc
     5941 cgaatcccta ctacgtagaa ggcatgcgcc cgctctccgg caacgatgct ccggtgcggg
     6001 attatgtgat gggatttgag acagcgcagg tgtttgcgga aaaattagag gatatgatcc
     6061 gttttctgat tccgaattat atttccgagg gcaagcatca gctcattatc agcatcggct
     6121 gcaccggtgg aaaacaccgt tccgtaaccc ttgccaacga gctgtaccgg cgtctttcca
     6181 acgcgaagga ctatggaatc cggattgagc accgggatct ggaaaaagac gccatccgaa
     6241 aaggaattta gaggtggaag gatgtcattc tcaagcgagg taaaagagga attatcgaga
     6301 catcttccga cggcacgcca ctgccagatt gcggaaacag cggcgatcct gtcactctgc
     6361 ggacagattt ccgagaagga cgggacgata cggattcaga cagagaatgc gtatgtggca
     6421 agaaagtact ttactttatt gaaaaaaaca tttaatatag aggcagagct tgcagttaag
     6481 ctgaatcaga aggaaaatac gtatctgatc cgcgtaaatg acagggaaga aacccgtcag
     6541 attctgcagg cagtgaagtt acacgagaca gaccggcagt tagcaatgcc gattccggct
     6601 gttgtggtac agcgtcagtg ctgcaaacgg gcatttctgc ggggcgcatt tttagcgtcc
     6661 ggttccatca gcgatcccaa ccggttttac catctcgaaa ttgtctgccc cgatgcaccg
     6721 agagcagaac aggtgcagga acttctgaaa agctttggaa ttgatgcacg gatcgttctc
     6781 cggaaaaacc accatgtggt ttatatgaaa gaaggcgcac agattgtgga gatgcttggc
     6841 atcatggaag ccaacatcgc acttatgaat ctggaaaacg tgcggatctt aaaagaaatg
     6901 cgcggcagtg tcaaccgaaa ggtgaactgt gaaactgcca acatcaacaa aacggtcagc
     6961 gcggcgatcc gccagatcga agatatccgg tatatcgaag aaaccacagg cttttcttct
     7021 ctgccggaac cgctggagca gatggcaagg ctccgtttac aatacccgga agcatcgctt
     7081 ctggaactgg gcaagatgtt aacgccggtt gttggtaaat ccggagttaa tcatagatta
     7141 aggaaactaa aagagattgc agaagatttg cgaaggaaac aaggaggaga agtattatga
     7201 ttaagaaacc aatcacaatt cgtctttcaa gcggcctgga ggcgagacca gttgccatgc
     7261 tggtacaggt agcgagccag tacgaaagtg aaatctatgt cgagagcggc agaaaaaaag
     7321 taaatgcgaa gagcattatg ggtatgatga cacttggtct ggacaatggc gaggaagtta
     7381 ccgtgaccgc aaacggaaaa gacgagagcg aagccatgga cggcgttgaa aaatatttaa
     7441 gcagtccttt tgctgaataa tcttactgca cttatagcat aaacatagag caaaacggca
     7501 tgtcagaaaa ttttgacatg ccgttttgct atgctttttt atgtgaaatc agacgaaaat
     7561 cagaccacgt tcttcgcgcg gaaccagtag gtatagagat aggaaaaacc gagcgattca
     7621 tacagtgtga tcgcaggcgt gtttccctcg acaacctgaa gatacgcttt cgttgcaccg
     7681 agccggattc cctcggagag gattgtgcgg cagatcgagg aagcgtagtg gcggtggcgg
     7741 tacgcaggat gcacatggat ggcatagaca ccgacgtaat cgcggtcgcg gatgccaagg
     7801 ccggtggcaa cgatcgttcc ggtttcatcc tggatggagg tacagatggt ttcttttgga
     7861 atggcgcggt acatcgaagg aacgaccatg cggtgcatca cgttcgtcgt gctctttaaa
     7921 gcaaagaggc tgtcaatcca tttcggcggg ataaagctgc tgacggtcac ggcaacggca
     7981 ggattctttt tggcaaagtt cgccagatcc attgtcatga cgtgcgtcgt gtgctggact
     8041 acatagccgc ggtcggcgag cattgcatca aagccgggat tgacaagcgg actgatcttg
     8101 aaaattgccg gtgtacccca gcgacggtag atctcctcac agtactgcac tttttcctcc
     8161 accggaatcg tggacggccc gatctgctcg acgctgttgg tccggtgtgt ataaaaataa
     8221 gaaaaacgaa gaatccatcc gtcgtacagc tcgatctggt gagacggcca tgcgttcagg
     8281 gaaagatctt cgatggtttt gatcagtgta ttcatatgaa agtattcccc ctttggttat
     8341 gtatatagta tagaagaaac aggacagatc ttcaagaaaa aatcgaaaaa ttcgacacgc
     8401 ggaaccgggc ggccgggaca cgatgcgtaa cgttttcttg cataatcgaa gaaaaggggt
     8461 tataataggg ggcaaagact gagaatacaa ggagaattga atatgtcaaa attactgaag
     8521 gtaattgtga atacgctgct tctgcttgca atcctgtcag caggcgcact ggtgattccg
     8581 ccgtttgccg gcatcacgac acttgtctca gacgggatgg gaaataccaa catttcccgc
     8641 ggcgctgtca cgtacgcaaa gaagacagat acgtcatcgg ttgcgacggg agacaaaatc
     8701 ctggttgatg acaattccgg aaagtatatc tatacgatac agtccctcga caccgctgcc
     8761 ggaaccgcat ccgtaaaaaa tgtgatcagt ggagacacaa cggaaattac cgtgagagca
     8821 accgtgagca aagtgatcgt cacgattccg gtgatcggct ttctgtcgat ggcggcgcag
     8881 agcagagagg gaatcatgat catcggactt gccattctgt ttctggtcat tttgttcatc
     8941 ctttccgagg tgtggaaacg tgatgaagaa gaggatgagg aagaagagga agaagacgaa
     9001 gacgaggacg atgagaaagc gtccgaagag cttcgcagca gaaagaaagc tgccctggaa
     9061 gaaaaaaaga aacgggaaga acaggaaatg accgaacagt tgagagcagc cgaccagaaa
     9121 acggcagaag aagagaaaca ggacgaattt gaaattccgg atctggaaga agaaggaacg
     9181 gagcaggtgt cctttcatac cgtcgatctg cagaaccttg taagaggaga agaaaccgaa
     9241 gaaattccgg aagccgtgga agaaacggaa attgtcgaag aagctgccgc agaagaaaca
     9301 gcggaagaag tgacagaaga aaccgaagaa acggaatctg cagagccggg aactgtggaa
     9361 gaaatcgaag agtttgaaga aaccatcgaa gagccggaag aagagccgga accggaaccg
     9421 tccgagccga tcgaacttgc catgccggtt tacacggcgg aagaactcct ccagaaggcc
     9481 cacgcagccg gagatgaccc gaaagtcatt gaagatcctg caaccggcat cacaatctta
     9541 gattattccg acattctgta actattcaga gccatgcgaa cgaaaagaaa agaacgcgaa
     9601 tcatgcttgc atgaatgaac gttcttttct ttttgttcat atggcaagaa gccacatcga
     9661 gatgcatcgc agcggaatcg agatggggct ctgaatagtt ggaaaacgtc aaaaaagaga
     9721 gaaatatagg aaaagtgcac aacacggcac ccctgtattt ctctttttgc ataaaaacgg
     9781 ataaatcgaa aaaaataaaa aaaaaccttg atttttcgcg tgctttcttg tatactaaca
     9841 actgttgtga cattgatagc attgaagcgc gaggttgccg ctgagaaaca gcgggttttc
     9901 cgtggagcga atgtcaagtt aaagaaactg gcgacaagtc actgtaccca caattaagtc
     9961 cacctgtaag agtggaaacg accgtgtgca aacatcagga cacacacggg taagtgtaca
    10021 gtcaccgctt gtcgtactcg taaaagtgcg aaaaggaggc gacttttttt atggcaagtc
    10081 aagtaatgag aattacactg aaagcgtatg atcatcagtt agtagatgca tctgcacaga
    10141 aaattatcga aactgtaaag aagaacggag caacggtgag cggaccagta ccgcttccaa
    10201 cgaagaaaga ggtagtaaca atccttcgtg cagttcacaa gtacaaagat tcccgtgagc
    10261 agttcgagca gagaacccac aagagactga ttgatatcat cgcacctacc cagaaaacag
    10321 tggatgcatt agcaagactg gaaatgccgg ctggtgttta catcgatatc aagatgaaaa
    10381 acaaataaga tcagctgtaa aaagtccggg atcagccccc ggccatcaat taagtaagca
    10441 atagatgata tagaagcaac actcattcga aggaatcaac atccctaagg gtcatagtgt
    10501 tccacttata tcaactgttc gctagaatga acttctttat aataggaagt tccgctgtag
    10561 ataaaacagg aggtaaaagg aatgaagaaa gcaattttag ctacaaaagt cggaatgact
    10621 cagatcttcg acgaaaacgg tgttttagta ccggtaactg tattgcaggc aggtccgtgt
    10681 gttgtaacac aggtgaaaac cgcagacaac gacgggtaca gtgcaattca ggtaggcttc
    10741 ggcgagaaga aagaaaaccg tgtgaacaaa ccaatgaaag gccagttcgc aaaagcaggt
    10801 gtttcctgca agagatatct gaaagagttc agatttgaag atgctgaaac ctatacatta
    10861 ggacaggaaa tcaaggccga tatctttggc gcaggcgaca aaatcgacgc tactgcaaca
    10921 agcaaaggta aaggattcca gggtgccatc aagagacatg gtcagtccag aggacctatg
    10981 gctcatggtt ccaaatatca tcgtcacgca ggttccaatg gtgcttgctc cgatccgagt
    11041 aaggtattca aaggaaaaag aatgccagga cagatgggtg gaaagaaaat caccgtacag
    11101 aacctggaaa tcgtaaaagt tgacgtagag aacaatttac tgttagtcaa aggtgctgta
    11161 ccaggaccga agaaatccat ggttaccatc aaagagacag taaaatgcgg taaataagtt
    11221 cgttgaaagg aggaacacac gtaatggcta acgtatctgt attaaatatg gaaggcaaag
    11281 aagttggcag catggagctt aatgatgcag tgttcggtgt agaaatcaac gagcatctgg
    11341 tacatcaggc tgtagtgctt cagcttgcaa ataatcgtca gggaactcag aaagctaaaa
    11401 cccgttccga agtaagcggt ggcggaagaa aaccgtggag acagaaagga acaggtcacg
    11461 caagacaggg ttcaacaaga gctcctcagt ggacaggcgg cggagttgta ttcgctccag
    11521 taccaagaga ttactctttc aaaatgaaca agaaagagaa aagagctgca ttaaaatccg
    11581 ttctgacaag caaagttcag gagaacaaat tcatcgttct tgatgagctg aaacttgcag
    11641 aggttaaaac aaaagagatg aagaaagtgc ttgataacct caaagtaaac aacgcactgg
    11701 taatcatcgg agatgacagc gaaaacgtag cactttccgc aagaaatatt gccggcgttc
    11761 agactgcatc tgttaatacc atcaatgtat tcgacatgct gaaatacaac accatcatcg
    11821 caaccaagag cagtgttgca tctatcgagg aggtgtacgc ataatggcag atatcagata
    11881 ttacgacgta atcctgaaac caatcgtaac tgagaagagc atgaacgcta tgggcgagaa
    11941 gaaatacaca tttatggttc acccggaagc aaacaaatct cagattaaag aagcagttga
    12001 gaaaatgttc gaaggaacaa aagtaaaaag cgtaaacacc atgaacatgg acggaaagaa
    12061 caaacgtcgt ggaatggtag ttggaaaaac tgcaaagagc aagaaagcaa tcgttgctct
    12121 gaccgaggac agcaaagaca tcgaaatctt cgaaggactt taattagcag ctgttcgaaa
    12181 tatgcacaca taagtgcgat acgaaatgaa aggagaacaa agtaatggga atcaaaagct
    12241 ttaacccata tacaccttcc agaagacaca tgacctcctc tgatttcaac gaaatcacga
    12301 agagcacacc ggagaaatct ctggtagtgt ccctgaagaa aaacgctggt cgtaacaacc
    12361 agggtaaaat cactgtaaga caccgcggag gcggaagcag aagaaaatac agaatcatcg
    12421 acttcaagag aagaaaagat gatgttccgg caatcgttaa atcgatcgag tacgatccga
    12481 acagaacagc aaacatcgct ctgatctgct atgcagacgg cgagaaagca tacatcctgg
    12541 ctccggaagg actgaaagtt ggcatgaagg ttatgagcgg cgctagcgcc gaggtaagac
    12601 ctggaaactg cctgccgctg tccgagattc cggttggtac catggttcac aacgtagagc
    12661 ttcatgcagg caaaggcgga cagcttgttc gtgcagctgg taatggcgct cagctgatgg
    12721 ctaaagaagg caaatacgca acccttcgtc ttccgtctgg agagatgaga atggttccga
    12781 tcaactgtcg tgcatccatc ggtgttgttg gaaatggcga gcacagcctg atcaacattg
    12841 gtaaagcagg acggaaacgt aacatgggta tccgcccgac tgtacgtggt tctgtcatga
    12901 acccgaatga ccatccacat ggtggtggag aaggaaagac cggtatcgga cgtccgggcc
    12961 cgtctactcc ttggggaaaa ccggctcttg gcctgaagac cagagcaaag aacaagcagt
    13021 ctaacaagta catcgtaaga agacgcgatg gaaaagcttt atcgaaataa ggaggtaaac
    13081 gatgtctcgt tcattaaaaa aaggaccatt tgcagatgaa agcctgttaa agaaagttga
    13141 cgctgcaaac gcttcaggag ataaaacagt cattaaaacc tggtcacgtc gttctacaat
    13201 cttcccgtca ttcgttggac acacaatcgc tgttcatgac ggaagaaaac atgtgccggt
    13261 atacgttaca gaggacatgg ttggacacaa actcggtgag ttcgtagcaa ccagaaccta
    13321 cagaggtcac ggcaaggacg agaagaaatc cggcgtccgc taagtagaat gatttgagag
    13381 aggaggtttt ttagcaatgg ctaaaggaca tagaagtcag attaaaagag caagaaatgc
    13441 taacaaagat accagaccgt ctgcaaagtt atcctacgct agaatgtctg cgcagaaagc
    13501 tggatttgtt ttagatgcaa tccgcggtaa agacgtaaca accgcacttg gtattgtaac
    13561 ttataacccg agatatgcct ccagcgtagt gaaaaaactg ctggagtccg caatcgcaaa
    13621 cgctgagaac aacaacggtc tttccagaga aaacctgtat gtagctgagt gcttcgctaa
    13681 caaggctccg accatgaaga gaattcatcc gagagcacag ggcagagctt acagaatcct
    13741 gaagagaaac agccacatca cgattgtgtt agacgagaaa taaggaggac atgtcatggg
    13801 acagaaagtt aatccacacg gcctgagagt cggtgttatt aaagactggg attccaaatg
    13861 gtatgccgag ggtgatttcg cagataacct cgtagaggac tacaacatcc gcacatacct
    13921 gaagaagaaa ctgtacagcg caggtgtttc caagatcgaa atcgaaagag catctgacag
    13981 agtaaaagtt atcatctaca ccgcaaaacc gggcgttgtt atcggtaaag gcggagcaga
    14041 gatcgagaaa gtaaaagctg aagttcagaa actgactgac aaaaagctta tcgtagatat
    14101 caaagaagta aaaagaccgg accgcgatgc acagctcgtt gctgagaaca ttgcattaca
    14161 gctggaaaac cgtatttcct tccgtcgtgc tatgaagtct accatgcagc gtacgatgag
    14221 agctggcgct aagggaatca aaacatctgt atccggacgt cttggtggag ctgatatggc
    14281 tcgtaccgag ttctacagcg agggaactat tccgctgcag accctgcgtg ccgatatcga
    14341 ttatggattc gctgaagcag ataccactta cggtaaagtt ggcgtaaaag catggatcta
    14401 caatggcgaa gtacttccaa ccagaactaa aaaggaaggg agcgagaatt aattatgtta
    14461 atgccgaaga gagtaaaacg tcgtaaacag ttccgtggtt ccatgaaggg taaagccctc
    14521 agaggaaaca aaattaacta cggtgaattt ggtctcgtag cccttgagcc atgctggatc
    14581 aaatccaacc agatcgaggc agcccgtatc gccatgacac gttacatcaa acgtggtggt
    14641 aaagtttgga tcaaaatttt cccggacaaa ccagtaaccg cacagccggc tgaaacccga
    14701 atgggttccg gaaaaggttc tctggagtac tgggtagcag tagtaaaacc aggccgtgta
    14761 atgttcgaaa tcgcaggtgt tccggaagaa acagcccgcg aggcattacg tcttgccatg
    14821 cataagttac catgtaaatg caaagttgtt tctcgtgcag acttagaagg cggtgataac
    14881 agtgaaaact aacgaatttg taaaagattt aaatacaaag agcgcagctg agctgaatga
    14941 cgaattagta gctgctaaaa aggaactctt caatttaaga ttccagaatg caaccaacca
    15001 gcttgacaat acaagccgaa tcaaagaggt tcgcaaaaat attgcaagaa tccagactgt
    15061 tattgcccag aaggcaaacg cagctgagta attaggagga aaatatcgtg gaagaaagaa
    15121 atcttagaaa aactcgcgtg ggcaaagttg tcagcgataa gatggataag actatcgtgg
    15181 ttgccatcga agaccatgta aaacatcctc tgtacaagaa aattgtaaaa agcacatata
    15241 agctgaaagc acacgatgca aacaacgagt gccatgtagg tgataccgta cgagttatgg
    15301 aaacaagacc tttatccaaa gataagagat ggagacttgt ggagatcgtt gagaaagcga
    15361 aataaataag agatcagaat gctaaaggag gaataccagc atgattcagc aggaaactag
    15421 actgaaagtc gctgacaaca ccggtgcaaa agaaatctta tgcattcgtg ttatgggcgg
    15481 ttctacaaga agatatgcca acattggtga tgtaatcgtt gcttcggtca aagatgcaac
    15541 accaggcggc gttgttaaaa aaggtgacgt agtaaaagct gttgttgtaa gaagcgtaaa
    15601 aggcgctcgt cgtaaagacg gttcttacat caaatttgat gagaacgcag cagttattat
    15661 caaagatgac aaaactccaa aaggaacccg tatttttgga ccagtagcca gagagcttcg
    15721 tgagaaacag ttcatgaaga ttgtttcctt agctccggaa gtattatagg aggacagcta
    15781 tgtcaatgaa gattaaaaaa ggcgataccg taaaggtaat cgccggcaaa gataaagaca
    15841 aaaccggcaa agtagtcagc gttgacgtaa agaacaaccg tgttgttgtt gaaggcgtaa
    15901 acatgattac aaagcatgag aaaccgtcta tgagcaatca gcagggcgga atcgttaaca
    15961 aagaagctcc tatcgatgca tccaacgtaa tgctggttgt aaaaggagtt ccgacccgcg
    16021 ttggaatcaa agttgagatg gttgagaaaa acggcaaaac aaaagctgtt agaacccgcg
    16081 ttgcaaaagc aaaagagctg aacggcgcag taattgatta aggaagagag gaggcaaaac
    16141 cgtgagtaga ctgaaagatc tttacaacga gcagatcgtt gatgcaatga ccaaaaaatt
    16201 tggatacaaa aacgtgatgg aagtgccgaa gctctccaaa atcgtaatca acatgggtgt
    16261 tggtgaagct aaggagaacg ctaaacttct ggacgctgca atcagtgata tggaagctat
    16321 caccggacag aaagcagtta ccaccaaagc gaaaaattcc gtagcaaact ttaaaatccg
    16381 tgagggtatg ccgatcggat gcaaagttac ccttcgtggc gagaaaatgt acgagttcgc
    16441 agaccgcctg atcaacctgg cactgcctcg tgtacgtgac ttccgcggcg taaatccgaa
    16501 cgctttcgat ggcagaggaa actatgctct tggtatcaaa gagcagctga ttttcccaga
    16561 gatcgagtat gataaagttg ataaagtcag aggtatggac attatcttcg ttaccactgc
    16621 taacacagat gaagaagctc gtgaattact gacattattc aacatgccat tcgcaaagta
    16681 ataggaggaa ttgattcatg gctaaaacag caatgaaaat caaacagcag cgcaaagcta
    16741 aattctctac aagagaatac aatcgttgca gaatttgtgg ccgtccacac gcatatttaa
    16801 gaaaatacgg aatctgcaga atctgcttcc gtgaattagc atacaaaggc cagattcctg
    16861 gcgttaagaa agctagttgg taaaatttta gaaggaggca aattcaaatg agtatgagtg
    16921 atccgattgc agatatgctt acaagaatcc gtaacgctaa cactgcaaaa catgatacag
    16981 tagatgttcc ggcatccaaa atgaaaatcg caatcgctga tatcctggta aacgagggat
    17041 acattgcaaa atatgaactc agcgatgagg gtgcattcca gaatatgcac atcaccctga
    17101 aatacggcgc tgacaaaaac gagaagatca tcaccggtct gaagagaatc tccaaaccgg
    17161 gtctgagaat ctatgttggc aaagatgagc tgccgagagt acttggcgga cttggaatcg
    17221 ctattctttc cacaaaccag ggtgttatta ccgataaaga agctcgcaag ctgcaggttg
    17281 gcggcgaggt tctggcattc gtttggtaat ctgaaacgaa acaaagcaac tgaaaacaga
    17341 gagagcgtaa ggctctctcc gaaaatctaa gttaatagga ggaaattggt atgtcacgta
    17401 ttggaagact gccagtggca attcctgcag gtgtaacagt agacatccag gaaaacaacc
    17461 acgttgtagt aaaaggacca aaaggaacat tagagagaac tctcccggct gaaatgagca
    17521 tcaaactgga agacggacat gtagtagtta caagaccgag cgatctgaag aaaatgaaag
    17581 ctcttcatgg tctgaccaga actctgatcc acaacatggt agttggtgtt agcgaaggct
    17641 ataccaaaac actggaagta aacggtgtag gttacagagc tgcaaaacag ggcaacaaat
    17701 tagttctgtc tcttggatat tcccatccgg tagagatgat cgatccggaa ggactctcct
    17761 ctgctgtaga cggcaacaag atcattgttt ccggtatcag caaagagaaa gttggccagt
    17821 atgctgctga aatcagagat aagagaagac ctgagccata caagggcaag ggaatcaagt
    17881 atgctgatga agtgatcaga cgtaaagttg gtaagactgg taagaaataa ttaaggagag
    17941 tgtgaacatg attagtaaag tatcaagagc gcagatgcgc cagaaaaagc atagaagatt
    18001 acgtaatcat atcgtaggta ctgcacagcg tccgcgtctg tgtgtattcc gcagcaataa
    18061 ccatatgtat gctcagatta tcgacgatac gattggaaat actttagttt ctgcttctac
    18121 tcttcagaaa gatgttaaag cagagcttga gaaaaccaac aatgtagatg cagcagcata
    18181 tcttggaacc gttcttggaa agaaagctgt agaagcaggt attactgaag ttgtatttga
    18241 ccgtggcgga ttcatctacc agggaaaaat ccaggcattc gcagacgcag ctagagaagc
    18301 tgggctggta ttctaagaaa ggaaggaaaa gaacacatga agcatacaat tattgatgca
    18361 agccagttcg aactggaaga aaaagtcgta tcaattaagc gtgttacaaa agttgttaaa
    18421 ggtggtcgta acttccgttt cacagcttta gtagttgtcg gcgacggcaa tggacacgta
    18481 ggcgcaggcc ttggaaaagc agccgagatt ccggaagcta tccgcaaagg taaagaagac
    18541 gctatgaaga aactggtaag cgtagcaatc aatgagaacg gaagtatcac tcatgactac
    18601 gtaggtaaat tcggaagcgc agaggttctg ttaaagaaag ctcctgaagg tactggtgtc
    18661 atcgctggtg gcccagcccg tgccgtaatc gagttagctg gtattaagaa catccgtaca
    18721 aaatcccttg gatccagaaa taagcagaac gtagttctgg ctaccatcgc tggtctgtct
    18781 gagctgaaga ctccagaaga agtagcaaga cttcgcggca aatctacaaa cgagatcttt
    18841 gcttaaggag gatgtgaaaa atggcagata aattaaaagt aacactggtg aaatccccga
    18901 tcggatgtgt tccgaaacac agaagaaccg tggaagcact gggtctgaaa aaagttaaca
    18961 agacggtaga actgccggat aacgcagcag ttcgcggcat ggttaatcag gtttcttacc
    19021 tggttaaagt agaagaagca taaggaggta ccgtaatgga cttatctaat ttacagccgg
    19081 ctgaaggttc cagacagaac gataacttcc gcagaggccg tggacatggt tcaggaaatg
    19141 gtaaaacagc aggtaaaggc cacaaaggac agaaagcacg ttccggagca ccgagaccgg
    19201 gatttgaagg tggtcagatg cctttataca gacgtcttcc gaagagaggt ttcaaaaaca
    19261 gaaactccaa ggaaatcgtt ggaatcaatg tagatgctct gaatcgttat gaggacggag
    19321 ctgttgtaac cgtagaaact atgattgaga ctggtcttgt ttccaatccg agagatggcg
    19381 taaaaatcct tggaaacggt gaagtcacca aaaaactcac tgtaaaagca aatgctttca
    19441 gcgagagcgc aaaggcaaaa attgaagcgg caggtggaac agtcgaggtg atctaatgtt
    19501 taagaccttt atcaatgcat ttaaggtcaa agatgttcgt cgcaaaatat tttatgtgct
    19561 tatgatgctt gttgtcatcc ggttaggctc tcagatgcct gtaccgggtg tcaatacaag
    19621 cttttttgcg aattattttg caaataatac gaatgaggca ttcagcttcc tgaatgcatt
    19681 cacgggcggc ggtttcgacc gtttctccat ctttgctctg agtattacac cgtacattac
    19741 ttcttccatc attattcagc tgctcaccat tgcaattcca cgacttgagg aaatgcagcg
    19801 tgacggcgaa gagggaagaa agaaaatgac tgcgattact cgttatgtga cagtaggtct
    19861 tgcattgttt gagtctgttg ccatggcagt aggctttgga cgccagggac tcctcaccac
    19921 ttttaacgca gttaatgtga ttgttgtagt ggcagctctg acagccggtt ctgccttcct
    19981 tatgtgggtg ggagaacaga tcaacgacaa gggcgttgga aatggtattt ctatggtcct
    20041 gttaatcaac atcctttcca gaatcccgca ggatatggta accctttacg aaaccttcgt
    20101 aaaaggcaaa accatcgcgc gtggtctgct gatgtggatt atcatcgctg ttgtgatcat
    20161 cgctgttgtt gtgctggtac tgatcctgaa tggcgctgag agaagaattc cggtacagta
    20221 ttccaagaaa atggccggaa gaaaaatggt tggcggacag tcatccaata ttccgctgaa
    20281 ggtaaataca gcgggcgtta tcccgatcat ctttgcatca tccatcatgt ctttcccatc
    20341 gattatcctg tcgtttgtcg gaaagtcgga tatcaaaggc atcggcggaa cgctgatcaa
    20401 gatgctgaat tccaacaact ggtttgacaa aaccaatcca gttgcttcgc tgggcctgat
    20461 tctctacatt gtccttgtta ttttcttcgc ttatttctat acctccatca ccttcaaccc
    20521 gatggaagtt gcagataaca tgaaaaaaca gggcggcttt atcccaggca tccgcccggg
    20581 taaatccacg gtagattatc tgaacaatct gctcagctat attattttta tcggtgcagc
    20641 aggtcttacc attgttgcag tgattccatt tttctttaat ggatttttca aagcgaatgt
    20701 atcctttgga ggtacttctc tgatcattat tgccagcgta attctggaaa ccttaaaaca
    20761 gattgaatcc atgatgctgg tacgcaatta caaaggattc ttaaacgatt aaggcataag
    20821 agggactgtc tggataagac aggccctttc ttttgtttaa agcgctttta aagaggcttt
    20881 agcagagaaa atttttgcta ttgtggttta ctttatggta taataaggta aattacacgc
    20941 agcggattgc tgcaaattca aacatgggag gaaaagcgca tgaaaattat tatgttgggg
    21001 gcacctggtg ccggaaaagg tacacaggca aaacagatcg ctgcaaaata tgagattcct
    21061 catatttcaa ccggcgatat cttccgcgca aatatcaaag agggaaccga gcttggaaag
    21121 aaggcgaaag cattcatgga caagggagaa ctcgttccgg acgagctgac ctgcgatctt
    21181 gttgtagacc gtatttccaa accggacgca gcaaagggct acgtgctgga tggcttccca
    21241 agaacgattc cgcaggccga ggcactgacc aaagccctgg aagcacgcgg agagaaaatt
    21301 gactacgcaa tcaatgtaga agtaccggat gaaaatatcg ttcaccgtat gggcggacgc
    21361 cgtgcctgca tcggatgcgg agcaacgtat catgtagaat ttaacgcacc aaaggtagaa
    21421 gatgtatgcg atacctgcgg cggcgagctg gttcttcgtg atgacgataa acctgagacc
    21481 gtacagaaga gactgtctgt ataccatgct cagacacagc ctctgattga ctactataag
    21541 aaagccaatg ttctggtgga agttgacgga acccaggata tcaacgtggt atttcaggat
    21601 atagtaaaga ttttaggagc gtaaaggatg tccatatcaa ttaaaactgc ccgggaaatt
    21661 gaaaaaatgc gccgtgccgg tgaactgctg gagaaggtac acgatgaatt aggccgcgcc
    21721 atccatccgg gaatgtcaac ctgggaaatc aacaagctcg gagaagagat gatccgcagc
    21781 ttcggatgca ctcccaactt tttaaactat aatgggtatc cggcttcgat ctgcgtttct
    21841 gtgaacgaag aagtggttca cggaatccca agtaaaaaac ggattctgaa agagggcgac
    21901 attgtcagcc tggatgcggg acttatctat gaaggatacc attccgacgc agcccgcaca
    21961 cacgcggtcg gtgagatctc gccggaggca agaaaactgg tggaagtaac acgccaggcc
    22021 ttttttgaag gaatcaaata cgcaaaagca ggccatcatc ttcatgagat ttcggcggcg
    22081 atcggtgcat acgcagagag cttcggctac ggcgttgtcc gcgacctggt aggccacggc
    22141 atcggaacgc acctgcacga agatccggca gtgccgaact tcgcacagcc aagccgcggc
    22201 gtccgcctgc agcctggcat gacgctcgcc atcgagccga tgatcacagc cggaggctat
    22261 gcagtcagat ggctggatga tgactggacc gttgtgacag aagacggctc tcttgccgca
    22321 cattatgaga acacgattct gattacggag ggagaaccgg aaattctgac actttcgcat
    22381 acggaggagg atttatgaaa ccgattgtga aaggaatgtt tgccagaagc ctggccggcc
    22441 acgataaagg aaggctgtat gtgatttcgg atgtacagga gccatacgta ttcctggtgg
    22501 acggaaaaat ccgtaccgcc aaccggccga aaaagaagaa gctgattcac gtacagccgg
    22561 attacgaaat gacggatgcg gttcgtgatg aagatatccg taaggcatta aaagaaaaaa
    22621 gttgcaaaga gttgaaggag gattaatcaa tgtctaaggc agatgtaatt gaggtagagg
    22681 gaactgtcct cgagaaatta ccaaacgcaa tgtttaaagt agaactggag aacaaacacg
    22741 tagtattagc tcatatcagt ggaaagctgc gtatgaactt tatccgtatt ctcccgggag
    22801 acaaagtaac catcgagctg tccccatacg atctggacaa gggacgtatt atctggagag
    22861 ataaataaga aatacgataa aaaaagccat ccggcatgcc ggatggcttt ttctctgtat
    22921 gtgtttctct gtattcatcc ccaataacag taaccgttct acagaacaaa gatttcgatc
    22981 accggccttc agatttctga cagccacaaa gcaagcacca gtctgcaaac cctgcagcat
    23041 ccgcggcggc gggcatctga atttcttttc agtccctttt gaaaccttcc agatgtatta
    23101 tgcaatattt taatagaaaa agcaatgaaa aaagtataaa attttatgaa aactttttgt
    23161 aaaaaccagg agaatttgtg cacttttgaa acaaaatttt taaaccgtca gaagaaaaag
    23221 aaaaagtgaa aagacaggag aaaagagcgg ccggaaaacc gtgaaaatga cagatgaggg
    23281 ctcgaaagcg acagaagaat atatgttcgt gaattctgtg tgaagttgaa atatttggtt
    23341 gaattctcaa aataatgtga tacaatgcaa tagagaaact ttacggggaa attttaccgg
    23401 gaagggtctc ctgggcggtg cggtaaaatc catgcaccgg cagaaatcag ggaaacattt
    23461 tatgaaacag caggctgcag tctgaaaaat aattcggatc tgcagcaatg gcatcgctgc
    23521 tccttacaga taacaggaaa ggaaaatgtg caatgacaaa aatgggaaag aaaaagaaaa
    23581 aaggccttgt tgcagcattg agcgtagttg cagtgattgt tttacttgcc gctgcatatg
    23641 gaatcttctg tctgatcatc gaggatgaca aaatctgggc agatgcttct attaacggag
    23701 taaacattca gggaatgagc aaaaaagaag ctgcacagac agtggaacag aagtttgaag
    23761 aagattataa agatacagct gttaccgttg agctggatgg tcagcagtat acgatgaacg
    23821 tatttccaat gctggggatg gatgcttccg cagaaattga aaaagcatat gaaaaaggcc
    23881 atggaaatct tctggttcgc ggtctggagt gggtcgagat gaaacgggga aaggcagaga
    23941 aactttccta tgatgtgcag ccgactgtgg cccatccaga tgaggtggaa ggaattgtac
    24001 aggcatccgg cattcaggat tacaactcca tgcaggatac tacatatgaa gtaacagata
    24061 ccggcctgat cgtgcataaa ggcattgccg gaaccagacc ggatgtggat gctttaaaac
    24121 agcagatcca gacgaatgtg acagcaatga atttccagga tgtaatttcc tgcccgacgg
    24181 cagaaaatca gctgacggag ccggattttg ccggaattgc aagccagatt tataccgagc
    24241 cggtcaatgc gacgctggat ccggacaacg gatattccgt tgtggcatcg aaaaacggaa
    24301 cctcaatgga tgcggacagt gcgaaagccg agtatgagag tgcagaagaa aatacggata
    24361 tcacgattcc gtttaccttt accgagccgg agattacaac cgagaagatg aacgcaaatc
    24421 tgtttaaaga cacgcttgga agttacagct ccagcgcagc aggtggaacg agcggaagaa
    24481 ttcacaacgt cggacttacg gcatctatct gcaacggaca gatcgttctg ccgggagaaa
    24541 ccttctcttt taatggtgtc gtaggtgata caacggcaga aaaaggatac caggaggcag
    24601 caggctatca ggatggcaaa gtcgtccagg tacttggagg aggcgaatgc caggtgtcct
    24661 ccacactgtt ttccgctatt ttgtatacag accttgatgt gttggaaaga gccaaccatt
    24721 cgatgccggt acattatgtg ccgtcaggaa tggatgcaac cgtattctgg gattcaccgg
    24781 atttcaaatt tgaaaataat cataagtatc cggtgaaaat tgtgatgaat atggacagca
    24841 gtgatacact gaccgtaaaa attctcggaa caaaagaaaa cgactatacc attgagccaa
    24901 gggtagagca gatcgatgag atgagtaatg taacgtatcg tgattataaa gatgcaagcg
    24961 gaaatgtagt aaaatcagaa tccgtatgcg caagcaaata caaaccgctg aactcaggat
    25021 catgatgcaa aaaagatgtt acctgtaaaa aacaggcggg acagagaaga gaaatctgtc
    25081 ccgcctgttt tttttcttac cggtgggaga gatgaaaaaa tgcacagaaa aagagcaaaa
    25141 aaaaatgaaa aaatatgtaa aaaatttctt gcatttccgg gaacaaaatg ctatactata
    25201 aaacgaactt ttgcgcgtgg acttttttct aacccacaaa aagaaaacgc attccaaaag
    25261 tttacaacag aaaattcccc tacacggggc gctaggagca aagaaaggag gattcatcat
    25321 gaaggttaga tcttctgtaa aaccgatctg cgagaaatgc aaagttatca aaagaaaagg
    25381 tagcatcaga atcatttgtg agaatccaaa gcacaaacag cgccagggct aatccgtcat
    25441 taggcttgat cgtaattgtg cagattttaa gtgcggctgc agcgaggacg cgcgtgaatg
    25501 cgttgttctg ctgttaatat tataaagtga gacacctggc aagttgcagc caggacacct
    25561 ccggcgggtg gacgcgcgga gcgcagacat cccttataat attgcttaat actccgatct
    25621 gtcaggtggc agaaggagaa aggtcgtgcg aacacggctg gatacacgca tacctgtatc
    25681 ccaattagga aaatattaat gttttagaaa aatggaggac aaagtacatg gctcgtatag
    25741 ctggtgtaga cttaccaaga gacaaacgtg ttgagattgg tctgacctat atctacggaa
    25801 tcggtagaac aagtgcagat cgtatcttaa aagaagctgg agtaaatccg gacacacgct
    25861 gcagagattt gacagacgat gaagtaaaga gactcagcgc agttatcgat gagagtcagg
    25921 ttgtagaagg agatcttcgt agagaaatcg ctatgaacat caaacgtctg caggagatcg
    25981 gatgctacag aggtatccgt catcgtaaag gccttccggt tcgtggtcag aaaacaaaga
    26041 ccaacgcaag aacccgcaaa ggtcctaaga gaactgttgc aaataagaag aaataattta
    26101 accagtagtc caatataaga ttaagaaagt gtaggttagt tttaaaatgg caaaagtgac
    26161 aaagaaaacg acaaaacgtc gtgtcagaaa aaacgttgaa cacggacagg cacatatcca
    26221 gtcttccttc aataatacaa tcgtaacact gactgatgca gaaggaaatg ctctttcttg
    26281 ggcaagtgcc ggcggtctgg gatttagagg ttcaagaaaa tctactccat acgcagctca
    26341 gatggctgca gaaactgcta ccaaagcagc gctggttcat ggcttaaaga ctgttgacgt
    26401 tatggtaaaa ggaccaggat caggtcgtga agctgcaatc cgtgcccttc aggcatgcgg
    26461 tcttgaagtt acaagcatcc gcgacgtaac accggttccg cacaacggat gccgcccacc
    26521 aaaacgtaga agagtctgat taggaggtta agaacatggc agtaaataga gtaccagtcc
    26581 tgaaaagatg cagatccctc ggaatggatc ctgtatatct tggaattgat aagaagtcca
    26641 caagacagct gaaaagagca aacagaaaaa tgtctgagta tggtcttcag ttaagagaaa
    26701 aacagaaagc aaaattcatc tacggcgttc tggagaaacc ttttgttaac tattacaaaa
    26761 aggctgacag aatggaagga cagactggtg agaacctgat gatccttctt gagctccgtc
    26821 tggacaacgt tctgttccgt atgggaatgg ctagaaccag aaaagaagca cgtcagatcg
    26881 ttgaccacaa acacgttctg gttaacggca aacaggtaaa tatcccgtct taccttgtaa
    26941 gcgctggtga taccatcgaa atcaaagagg gtaaaaaatc ctctcagaga tataaagaca
    27001 tcgtagaggt tacaggcggc cgtctgactc cagaatggat cgaggctgat cttgaggcac
    27061 tcaaaggtgt tgtaaaagag cgtcctaccc gcgaaatgat cgatgttccg gttgacgaag
    27121 tgcagatcgt cgagctgtat tctaaataaa gaatcgccat acgcggaagg ctttattccc
    27181 atgagtaatg agccaaccaa tttaaaagga ggggctttcg agtgtttgat tttaacaaac
    27241 cgaaaattga aattactgag atttctgatg acaagaagta cggcagattt gtggttgaac
    27301 ctctggaaag aggctacggt atcacattag gtaattctct tcgcagaatt atgctttctt
    27361 ccctccctgg agcagcagta agccaggtta aaatcgaggg cgttcttcat gaattcagct
    27421 ccatccctgg agtaaaagaa gacgttacag aaatcatcat gaacctgaag agccttgcaa
    27481 tccgcaataa cagcgaaacc aacgaagcaa aagttgctta tattgagttc gagggcgagg
    27541 gagttgtaag agcatccgat attcaggtag atcaggatat tgagatcatg aatccggatc
    27601 aggtaatcgc aaccctgaac ggcggcccgg acagcaagct gtacatggag ctgacgatca
    27661 caaaaggccg cggttatatc agtgctgaca aaggcaagag cgatgacatg ccgatcggcg
    27721 ttctcgctgt ggatgctatc tatacaccgg ttgagcgtgt aaacctcaaa gtggagaaca
    27781 cccgtgtagg tcagattacc gattacgata aactgacact ggatgtttac accagaggaa
    27841 cactggatcc ggacgaggca gtcagcctgg cagctaaggt tttaagcgag catctgaaac
    27901 tcttcatcga tctgtctgag aatgcgaaga cagcagaagt tatgatcgag aaagaggaca
    27961 atgagaaaga gaaagttctg gagatgaaca tcgacgagct tgagctttcc gtgcgttcct
    28021 ataactgctt gaaacgtgcc ggtatcaata ccgtagaaga gctgtgcaac aaaacttccg
    28081 aagacatgat gaaggttcgt aacctgggcc gcaagtctct ggaagaggta cttgcaaagc
    28141 tgaaagaact gggactgtca ctgagtcctg gggacggaga ataaatcaca gcgatttatc
    28201 tttccgtttg atttgtagac gaaaaccccc tgtcagtaag tccgaggcag catgacttgc
    28261 gggttccaca aatggaggaa aatttaaaat ggcaaaatat agaaaacttg gcagaacatc
    28321 tgaccagaga aaagcattat taagaaacca ggtaaccaac cttctgtatc acggaaaaat
    28381 cgttaccact gagaccaaag cgaaagagat ccgcaagatc gctgaaagca tgatcgcact
    28441 ggctgtaaaa gagaaagata actttgagac cgttaccgtt actgctaaag tagctcgcaa
    28501 agatgctgat ggcaaacgtg tgaaagaagt aaaagacggc aaaaaagtta ccgtatacga
    28561 tgaagttcag aaagagatca aaaaagatct tccgtccaga cttcacgcta gacgtcagat
    28621 ggcaagcttc ttctatcctg taacagaggt tccgaccgaa gcagcaggac gcaagaaaaa
    28681 caccaagtcc atcgatatgc cacagaaaat gttcgatgag atcgctccga aatatgcaga
    28741 ccgtaacggc ggatataccc gtatcatcaa gatcggaccg cgtaaaggtg atgcagctat
    28801 ggaagttgta atcgagctgg tttaatctgt aaatagtttt gaaattcgca ggggctgcct
    28861 cttagtaggt agctcctgtt ttgttgcata tcatatttta tatgacacaa ttgcgaaatt
    28921 gtggcaatgt acggggttgc atctgctttg cgaataaggt acaatacaga atacagaaca
    28981 gctaaaatgg gatacaaagg agagcatttg catggagagc aagaaaaaaa tgttgtttat
    29041 ttttaatccg ttttccggaa aagcacagat taaaagtaaa ctgtttgaaa ttatcgatgt
    29101 ttttgtaaaa ggcggatatg aagtcattgt ccacccgacg caggcagtcg gagatggttt
    29161 cgaaaagaca aaggagcttg cgccgcaggt ggatctcgtt gtctgcagcg ggggcgacgg
    29221 aacactggat gaagttgtca gcggtctgat ggaagtggat cagcgcgttc cgatcggtta
    29281 tattccggcc ggaagcacca atgactttgc aaacagtctt tctatttcga aagatatggt
    29341 acaggcggca aaggatatca tcgagggaaa cctgtattcc tgtgatgtgg gagcgttcaa
    29401 caacgacagc tttgtctaca tcgctgcatt cggccttttc acggatgttt cctatgagac
    29461 agaccagcac atgaaaaaca ttctcggcca tctggcatac ctgctggagg gatccaaacg
    29521 gatctggaat gtgccgacgt actggattaa agtcgaggca aacggggaaa cctttgaggg
    29581 agagtatatc tacggtatgg tgacgaatgc caagtccgtc ggcggcttca aaaacctgcc
    29641 gggacaggat gtacgcctgg atgacggact cttcgaggta accctgatca aacgcccgaa
    29701 aaacccgctg gaactgaatg agatcattgc gtccctgctg atgcaggagg atcacacaga
    29761 cctcatcgat tcgttcaaga ccgaccgcat catcatcgag tccaaccagg agatttcctg
    29821 gactctggac ggcgaatacg gcggagatca cggctatgtc tgcatcgaag cgcgcaaaca
    29881 tgccatggaa ctggttctga acaataagta ggcacggctt tttgataagt ttttcacaaa
    29941 aaagtgcaaa aaaatggatt tcgccacgaa aaccatttgg catttgcaga aagatgttgt
    30001 ataataaaac cagttatgaa agtaacaagt atgccgtaag cctgaaaacg gtacacggca
    30061 caattacaga cacaggaggt catttgagct atgttagtat cagcaaaaga aatgttggac
    30121 aaagcaaaag ccggccacta tgcagttggt cagtttaaca tcaacaacct ggagtggaca
    30181 aaatccatcc ttcttaccgc acaggagatg aattctccgg ttatccttgg tgtatctgag
    30241 ggtgcaggaa aatacatgac aggtttcaaa actgtagctg ctatggtaaa agctatgatc
    30301 gaagagctga acatcactgt tccggttgct ctgcatctgg accacggaac ctacgaagga
    30361 tgctacaaat gcatcgaagc aggtttttca tccatcatgt ttgacggatc tcactatccg
    30421 atcgaagaga acgttgcaaa aacaaaagaa ctggtagctg tctgcaaaga gaaaggaatg
    30481 tctctggaag cagaagttgg ttctatcggt ggagaagaag atggtgttgt aggtatgggc
    30541 gaatgcgctg atccgaacga gtgcaagatg attgcagacc tcggtgtaga tatgcttgca
    30601 gcaggtatcg gcaatatcca cggtgtatac ccggctaact ggcagggact gagctttgag
    30661 actctggcag ctgtacagga gctgacagga actatgccgc tcgttcttca cggtggtaca
    30721 ggtatcccgg atgacatgat caagaaagcc atcgatcttg gcgtttccaa aatcaatgta
    30781 aatacagagt gccagcttgc attcgcagca gctacacgta aatacatcga agagggaaaa
    30841 gatcagcagg gtaaaggatt cgatcctcgt aaactgcttg ctcctggcgc agaagctatc
    30901 aaagctactg taagagagaa aatcgaactg ttcggttctg caaacaaagc ctgagtttaa
    30961 gcacgaaaat gctgtgcagg attcctgggg gacttcctcc tcagggtccg cacagtattt
    31021 tttttcgcga gcaacgagtc aaagtccgtg cttgcacgag accttgacga ctgccgcgaa
    31081 agctttcatc gaatccgcta attttttcgc ctgaattagg aaagttttgt aaaataatac
    31141 gaaaaaggga ctgagttcaa aaaagcactt gcgtttttta agtttttgag ttattttagt
    31201 atcagttgga aatcaaatcc atatgcgaat gtaaaacaag ggcgttctac aaaaacttag
    31261 ctttccttag ctgctttttg agaacccctt tttcttgtat caggaccttt ttagagtccg
    31321 gcgggaaggt cgcatcgcaa aattcacaaa tggaaaggaa gaaagaaaat gggagaatat
    31381 gtaaatccgg ccaaaatctt tggcgaaaat gtattcaatg acactgtaat gcaggaacgt
    31441 cttccaaaaa atgtttacaa gaagctgaaa aagatcatcg aagaaggcgg tgagctggat
    31501 cttgctactg cagatacgat tgcacatgag atgaaagaat gggcaattga aaaaggagca
    31561 acacactata cacactggtt ccagccgctg actggtacaa ctgcagaaaa gcacgattct
    31621 tttatcacag caccgatgcc gaacggcaaa gtactgatga gcttctccgg taaagagctg
    31681 atcaaaggcg agccggatgc atcttctttc ccgtccggtg gactgcgtgc tacctttgag
    31741 gcaagaggat atacagcatg ggactgcacc tcaccggctt tcgttcgtca ggacgcagca
    31801 ggcgcaaccc tctgcattcc gactgcattc tgctcctaca caggagaggc gcttgaccag
    31861 aaaacaccgc ttcttcgttc catggaagct ctgaatgtac agtccctgcg tctgttaaaa
    31921 ctgttcggaa acaccacttc caagaaagta actccttccg taggaccgga gcaggaatac
    31981 ttcctggtag atgcagagaa attcctgcag agaaaagacc tgatctacac cggccgtacc
    32041 ctgttcggcg caatgcctcc aaaaggacag gagatggacg atcactattt cggaaccatc
    32101 cgtcagagaa ttgcttcctt tatgaaggag gtcaacattg agctgtggaa gatgggcgtt
    32161 accgcaaaga cacagcacaa tgaggttgca ccggcacagc atgagctggc ttccatctat
    32221 tccgaggcaa acgtagcagt cgacaacaac cagctcgtaa tgcagacctt aaagagagtt
    32281 gcatgccagc acggactgaa atgcctgctg catgagaaac cgttcgcagg tgtcaacggt
    32341 tccggaaaac ataacaactg gtccatcacc acagatgacg gcatcaacct tcttgagccg
    32401 ggcaaaacac cgcacgagaa cattcagttc ctgcttgtgt taagctgcgt cctgaaagct
    32461 gttgatgttc acgcagatct gctccgtgag tccgcagccg atccaggaaa cgatcacaga
    32521 ctgggagcca acgaggcacc gccggccatc atttccgtat tccttggcga gcagctggag
    32581 gacgttgttg agcagctgat cagcaccgga aatgcaacca agagtaaaaa agagggcgtt
    32641 ctggagactg gtgttaagac acttccggat ctcaagaaag acgctaccga ccgtaacaga
    32701 acctcaccgt tcgccttcac cggaaacaaa ttcgagttcc gtatggttgg ttcccgtgac
    32761 tccgttgcag ctccgaacat cgtactgaac accatcgttg cagaagcatt ccgcgatgca
    32821 tgcgatgtac tggaaggcgc tgaaaacttc gaggacgctg tccatgatct gatcaagaag
    32881 aacttaagtg agcatcagag aatcatcttc aatggcgacg gatatgcaga tgagtggtta
    32941 gctgaggcag agagaagagg acttccgaac atcaaatcca tggtgtacgc aatcccggca
    33001 ctgaccactg ataccgcaat caaactgttc ggcgatttca aagtattcac cgaggcagag
    33061 ctggtatccc gtgcagaagt taaattcgag aactatgcaa agaccatcaa catcgaagca
    33121 aagaccatga tcgacatggc aagcaaacag atcatcccgg cagtcatcaa atatgcgact
    33181 tccctggctg gatccatcaa caccatcacc gcagcaggtg ttacggcagt tggtgtacag
    33241 aagaatctgc tgaacgagac aagtgcactg cttgaggaaa ctcagaaagc actcgacgag
    33301 ctgatcgcaa tcgagaatgc aggatgcgag atggaagacg gcgaagcaaa agcaaaatac
    33361 tactacgaga aagttgctcc ggctatggag gcactccgtg caccggttga caaactggaa
    33421 atgatcgtag ac
//
LOCUS       DAOMUF010000006        29772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_190_length_29772_cov_3.035502, whole genome shotgun sequence.
ACCESSION   DAOMUF010000006 DAOMUF010000000
VERSION     DAOMUF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 29772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29772
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190_length_29772_cov_3.035502"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /gene="cbiB"
                     /locus_tag="PLN77_01060"
     CDS             complement(<1..673)
                     /gene="cbiB"
                     /locus_tag="PLN77_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HRM42901.1"
                     /translation="MKTALAALIVGYGLDLLLGDPSFLYHPIRVIGNLIALLEKWLRK
                     VFPKTPKGELAGGVFLVILVCLAGYGVPALLLFAAFKIHPVIGFLLEVLWCWQIPATK
                     CLKDESMKVYQKLKENDLPGARYAVSMIVGRDTENLSETGVTKAAVETIAENTSDGVI
                     APLLFLAIGGPAVGFLYKSINTMDSMVGYKNDKYLYFGRCAAKLDDIVNYIPARISAW
                     LMILAS"
     gene            complement(670..>1413)
                     /locus_tag="PLN77_01065"
     CDS             complement(670..>1413)
                     /locus_tag="PLN77_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HRM42902.1"
                     /translation="ASQMKETLDIDGILKIAENASENEIHYKEKKKRNLRIAVAKDEA
                     FCFFYRDNLELLASLGAKIEYFSPLSDAEPPKNTDGFLFCGGYPELHAEELSENQTMR
                     TKIRDNIRQGMPVLAECGGYMYLSESMEDMDGNVYPMVQAVPGNVYRTKKLGRFGYIT
                     LTPNTKDTFRNGGNPVRGHEFHYFDSTDCGSAWHAAKPLRKRNWECIHATKRMMAGFP
                     HLYYMASPHLALEFLEQCVSYRKEQEKTR"
     assembly_gap    1414..1423
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1424..2204)
                     /locus_tag="PLN77_01070"
     CDS             complement(<1424..2204)
                     /locus_tag="PLN77_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM42903.1"
                     /translation="MKAPRIMLAAGRSGSGKTMITCGLLRALQQMGKQPAAFKCGPDY
                     IDPMFHEQVIGAPSCNLDPFFTEEETTQYLFCKHAAGHDISVLEGVMGYYDGLGGIST
                     KGSAYDLAGMTKTPVLLVVDAKGMSVSALAFIKGYLQYKEDSRIAGVIFNRMSEMLYR
                     EIAPMAEKELGISCVGFVPELKECHLESRHLGLVMPDEVENLQEQVNLLAETLQKTLD
                     FEKILAIAEGAEELSGKMPEVLRKVTESEAAAKVRAAKPILA"
     gene            complement(2201..4180)
                     /gene="cobK"
                     /locus_tag="PLN77_01075"
     CDS             complement(2201..4180)
                     /gene="cobK"
                     /locus_tag="PLN77_01075"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HRM42904.1"
                     /translation="MNKVIVFAGTTEGREICSFLKKYEIASLACTATEYGKCRIEEGG
                     ALTVRSGRLTEEEMEQLFGEEQPEMVIDATHPYADLVSENIRSACEKAGKTYLRLLRP
                     SLAADPSCVMVDTTEEAVSYLAGTEGKILVTTGSKELAKFAELPDYKNRVYARVLPLP
                     GVIASCAELGFDAAHTIGMQGPFSMDMNVAMLQSFHAEWMVTKESGRNGGFEEKIEAA
                     KKAGVRVVLIGRPKEEEGLSEDEVKRYLVEKFDLSVKRDVVIAGIGPGAEAQMTLALV
                     DACEQADVLIGAGRMTKAVEKYQKPMLAEYSPEKIYEYLKAHPEYEKIVLLQSGDVGF
                     YSGAKKLQAVLAEDADFSVRVEPGISSLVYFCAKIGVSWEDAAFVSLHGQHCNFLETV
                     RRNRKTLAIAGGKGSVREICQTLSSYGWEQIVLHVGTDLSYPEEQIVTGTPFELAKME
                     FAPLAVLLFENPNPEPEFTAGMADEVFLRDKVPMTKREVRNASLSHLQLSSNSVVYDI
                     GSGTGSVSIEMALRAAQGKVYAIEKNPAAVALLQENKKKFAVDHLEIIEGTAPEALEA
                     LPVPTHAFIGGSAGNLKEILELLLRKNPNIRVVLNTVTVETLAEAASCFKKLAFEEPE
                     IVCLQASNAKKAGRSHLMIAQNPVYIFTAQGGAKE"
     gene            complement(4173..4904)
                     /gene="cobJ"
                     /locus_tag="PLN77_01080"
     CDS             complement(4173..4904)
                     /gene="cobJ"
                     /locus_tag="PLN77_01080"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HRM42905.1"
                     /translation="MSTIFVVGIGPGAYEKMTIEAADALRNCDVIIGYTVYVDLVKEH
                     FAGKEFLTTPMRKEVERCRLAFETAAEGKQVAMICSGDAGVYGMAGLMYELSEEWPDI
                     EIKVIPGVTAATGGAAVLGAPLIHDFALISLSDLLTPWEKIEKRLLLASEADFVICLY
                     NPGSHKRADYLEKACDLMLRYKAPETVCGIVSQIGRDGETSRTMTLAELRNTKVDMFT
                     TVFIGNSQTKEIRDHMVTPRGYRYE"
     gene            complement(4901..5932)
                     /locus_tag="PLN77_01085"
     CDS             complement(4901..5932)
                     /locus_tag="PLN77_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HRM42906.1"
                     /translation="MKIAIASFTRNGCVWNQKLCAALKKHSCEGYALEKYGKEAGIPA
                     IAPSLPAWTERMFQKMDAILFIGACGIAVRSIAPYVKSKKTDPAVLCMDEQGKFVISL
                     LSGHIGGANELTEEVAAVTGAVPVITTATDVNHKFAVDVFAVKNHCEICEMELAKEAA
                     ALLVDGKTVGFCSRFPVDGTKPEELIPEEAAKPSMGICITTSEEDSPYERTLHLIPKV
                     ITVGIGCKKNTPAGRIEEKVLAALAAAGISPKAVRQAASIDLKAQEPGILQLVEKYGW
                     QYRTFSAEELETAEGEFTPSPFVKKITGIDNVCERSAVLAGGRLIKKKKAADGVTVAL
                     AAADWRAVF"
     gene            complement(5934..6692)
                     /gene="cobM"
                     /locus_tag="PLN77_01090"
     CDS             complement(5934..6692)
                     /gene="cobM"
                     /locus_tag="PLN77_01090"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HRM42907.1"
                     /translation="MVHFIGAGPGAPDLITVRGQKLLGEADIIIYAGSLVNPALLDLK
                     KEGCVVYNSATMTLEQVIDVMKEAESKGQTVARLHTGDPCLYGAIREQIDALDALGIA
                     HDDCPGVSSFCGAAAALEMEYTLPDISQSVVITRMAGRTPVPERESIASFAAHQATMV
                     VFLSTGLLEELSRELIAGGYTADTPAAIVYKATWPEEKTFVCTVGTLAETAKKNQITK
                     TALMIIGNAVSQGNYRRSDLYNPAFTTEFREAEK"
     gene            complement(6693..7385)
                     /gene="cobI"
                     /locus_tag="PLN77_01095"
     CDS             complement(6693..7385)
                     /gene="cobI"
                     /locus_tag="PLN77_01095"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HRM42908.1"
                     /translation="MTGILFGTGIGPGDPELMTLKAVRLIRENDTIAVPGEKPEESIA
                     YQIAVQAVPELAEKELVAIPMPMTKNAKELEENHRRGAEKIEELLDAGKNVVFLTLGD
                     PTVYSTYLYVHRRVLEDGYDARIVSGVTSFCAAAASLSEGLVENSEELHVIPASYQIE
                     DALELSGTKVLMKAGKKMPAVKQFLKEKNCRAVMVENCGMDTEQKYFSAEEIPDQASY
                     YSLIIVKEKRKK"
     gene            complement(7393..8565)
                     /gene="cbiD"
                     /locus_tag="PLN77_01100"
     CDS             complement(7393..8565)
                     /gene="cbiD"
                     /locus_tag="PLN77_01100"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HRM42909.1"
                     /translation="MSRTGLEDAYIMRGSKKLRMGYTTGSCAAAAARGAAFMLLSGKE
                     IQEVKIHTPKGIVLNLELLDIRRSAEKVSCAVRKDGGDDPDVTDKALIYAEVTFGTEE
                     GIVIDGGFGVGRVTKPGLDQPVGNAAINHVPRQMIRENVEEIQKKLDDFRALQVIISV
                     PEGEELAKHTFNPRLGITGGISILGTSGIVVPMSEEALISTIRVEMEMRKAQGDRVLL
                     VTPGNYGQDFLKTYPWVRADHSVKCSNYVGKTLEFAAELGFDAILFVAHLGKFVKVSG
                     GIMNTHSHEADCRAELLTAQAVRAGADLALAKKLLETGTTEEAVQILKEAGCLKETME
                     KVTEKIAFYMNYHIEGRVQTEAIVFSSNEGLLGETPGAGELLERLREQEEKENRKE"
     gene            complement(8700..10400)
                     /locus_tag="PLN77_01105"
     CDS             complement(8700..10400)
                     /locus_tag="PLN77_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRM42910.1"
                     /translation="MKELYTAAGIFVVLILLGVWMVQNNRNEQEKKLVQLRKKWGKKQ
                     EKRTAREKKEKLEACLRRKAGENFCVDAITWNDLDLDAVFDTVDHTVSVCGEEYLYTA
                     LRMPVTTTEIREERERLMKLFAENQKAREAVQKALLLMGKEKMNPPTEEIAVLMEAEP
                     GEKGKYLLMAGIAVLGIGLLFVLPPLGVAVLFASMVGNGLMHGRESKKLESALKAFGC
                     ILRLQRTARELGKEKISGLEMYQKRLEEQEKQLRPITRKCRTLVNTKESQGGAANMIF
                     AYFHTFFLLDLIEYSSVVSGVKSYEKAILQMAEDLGLLDFALSAASYRESLSYYCRPE
                     FLDEKKAGCRIDAEELYHPLLTHPVANSLYAEGGILLTGSNASGKSTFMKNMAVNAIL
                     AQALNTSLSKRYRGVVCRIMTSMALRDNLAQGESYFVVEVKSLKRILEASREKTPLLC
                     VIDEVLRGTNTTERIAASESVLAALRRANVLCLAATHDTELTYLLEDLYTNYHFEEQI
                     TAQSISFPYRLEQGPARGKNAIALLGNMGIDEKIVKQAERRAAEFETTGSWEKNKFLR
                     "
     gene            complement(10454..11287)
                     /locus_tag="PLN77_01110"
     CDS             complement(10454..11287)
                     /locus_tag="PLN77_01110"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HRM42911.1"
                     /translation="MEREKISRKRRGIAVLTLAALFFWYLPQIQVSRQIRKEQEEAVE
                     MYVREEKEENKEENEKSEEVQEQEPGSTDQNLFRTIDFAGLRSKNREICAWLWIPGCA
                     LDVPVAHGQDNAYYLTHDAFCRERIYGSIFAPCDTPEDFSERHTILYGHNMRDGTMFG
                     GLKKYREASWKQEFPYLYLYLPGEAWQCRIWSVEETDAKSDVYRLGAWSDADWNAWVQ
                     ERKENSIIFGSGEEKEVQKILTLSTCIGDGSSSQRLAVHAAVEMVFATKSGREEGSVL
                     E"
     gene            complement(11339..13981)
                     /locus_tag="PLN77_01115"
     CDS             complement(11339..13981)
                     /locus_tag="PLN77_01115"
                     /inference="COORDINATES: protein motif:HMM:NF037639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HRM42912.1"
                     /translation="MKRAKKSFWFLAGILLLLGFCFREDVLASSLTSLPSLSKAGIGE
                     VVSSDSVIDGRYDFTPLIGSETKIRFGSTDGSTWDNLFCKNGESHSKCCSTWWPAADV
                     KGWSNLRSFTPLESKKGKMYAEYTNVGECHGENVTMRIWLEDWQNTVVPSGYEDVDAV
                     VVAIDHNKPVIDIKGLLWIKVRFAYYDSKGNPLNVKGYFTLSDLDFKQGFYLADETEH
                     IYLTKEADARIVYDARTEAIWSARRGSEPDGGTTPENSEGWVTFTFEGNSQTMIFYDG
                     GTNDAVTGPGGGVKAPKKWPDNFVNDTSSTYNNWHGASRETGITVVPWNTSEFGYTAN
                     VPCHLSKVGDLVVKKMDAETKEAISGAEFTVYRWKNNTWSEFKKMNWVKKTGSYLLEG
                     ILDTDSENGKFRVVETKNPAGYAGSWEQEFSIDKEGMQTIHLEAENTRKTGSLKIKKT
                     EENSGKALSGATYQVIASGAITTVNGTVLFPDQAVAAVLKTDENGEAFKDGLEYGTYL
                     VRETAAPNGYVLDPTEKKITIGEQNAQITLTFTNWKNRFVLQKVSQGDGKVLQGAAFH
                     IWTKDGSFDETKKTDANGKIELTGLLDGVWYYQETASPEGYLLDSTCREFVVENGKID
                     GKSELSVAVENDGTHLTIEKIDAESGKRISGAKLVLKDMDGNRVDSWISGGSGPHELE
                     KLKPGSYVLVEEAAPDGYLAAEPVSFVLEAKQEVQTVTMKDLACETLTITKKIKADEI
                     TWAHGNPTFLFSVKGKDLYGKEHTYQCYLTFTKTQVEKTTDQDGYTEQSVQIRGIPAG
                     NDYRVQEKKVLRYSLMQVTGTKNVTVKKLEEPAYGKDPARVFSVSVNLCGHPKESEVV
                     FENQKYRWDDYGHNSIVKNRIPVE"
     gene            complement(13978..15150)
                     /locus_tag="PLN77_01120"
     CDS             complement(13978..15150)
                     /locus_tag="PLN77_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42913.1"
                     /translation="MKGKRVRIGCAAASLLMGISGTYAYYRSSVETINKIAVGDVNIG
                     IWEYEEDGDGEKKYEGPENGIVLPSQVISKIPRITNYAEPCYVRVKPVFDEEETQDYR
                     LGEEDLGGIEDVWLKAGEYYYYPKVLGKQESVDFFRTITIPSAWTSEAEGQVLSVEIQ
                     AEAVQAANVTPDFTAEAPWGEELEIEQCVHEADNTVSEVSQQSFLTVSYEGAARNLIL
                     APDNFFRNFPVLMPGDEWEDTFTLVNTTASRAEFFLRTELPEELSETEKELLSKVKLE
                     IREGDSVIYEGDLESEGLWAAKSLGICSPDERKEICFSIRVPSELGNEWALRKSGVKW
                     IFSVEGEDLPQEEGPSIWHSAPKTGLRDPSAAVPIGIAVTGAFLFLTAGRSERRKE"
     gene            complement(15147..15680)
                     /locus_tag="PLN77_01125"
     CDS             complement(15147..15680)
                     /locus_tag="PLN77_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42914.1"
                     /translation="MKKKTKRLFFLAGAAGCTMAAAIWLLPGTSAYFTDHESVWNRMV
                     FGHNTSTIDEEFPTPTPVPPGKSVVKRVKIRNEGSVACYIRAALIFSEPIEAIEGLDT
                     ENWVKGEDDYYYYKKPVSEGESTTELFTGIRVAKEMEQKELEVSVYEESVQIQKGNMV
                     FHGYREAWDAYERGGQT"
     gene            complement(15703..16407)
                     /locus_tag="PLN77_01130"
     CDS             complement(15703..16407)
                     /locus_tag="PLN77_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HRM42915.1"
                     /translation="MKKQRKIVFWAASLLLTGALAAGGTLAYLTDKDSVTNQFVVGKV
                     DIEGNEPSYAPDPDGKTNHIVPTQVIPKDPRIKNKGKNDSYVYMDVSIPIAKVIVADT
                     SGNRLNGGAAVDTELFSMNQLSKKWVLMYKKKQNNSMVYTYLYNEVLAAGKTTDPLFT
                     SVTAANIVEGQLDEKEVDIPINYYAIQALNTGEGSTAAERAAKAWEKYVNQNNGQKGQ
                     VTAPQSEPEQTEAPGI"
     gene            complement(16452..17021)
                     /locus_tag="PLN77_01135"
     CDS             complement(16452..17021)
                     /locus_tag="PLN77_01135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM42916.1"
                     /translation="MKQGYSETEGKCYPLFFACKNDSLDIRIDGREQMKAKGKLGLQG
                     FLSFLFLAAGWYLMQYFCAYVVLSGSMEPEIPTGSVVVVDGRKKEWNPGDVITYRRGN
                     MVVTHRIVEKSEEGYYTKGDANAEEDAGIVLEKQVIGNVIVALPWLGFGIIWLRQRGT
                     LFFLAVGAVMLFTIRQLWHGRKIMQENKL"
     gene            complement(17138..18007)
                     /locus_tag="PLN77_01140"
     CDS             complement(17138..18007)
                     /locus_tag="PLN77_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRM42917.1"
                     /translation="MVTAECHAHIFMDGVDYKKAAAAHENGPREDLIREHLAAYQKAG
                     ISYVRDGGDPYGAGVLARSLAPEYGITYRTPVFAIHRAGRYGKIVGRAFSDWKEYCTL
                     VREVRRAGGDFIKIMTTGILDFATKGHVTSEPLSREEVFAMTAAAHDAGLSVMAHVNG
                     AQAVMDAAEAGVDSVEHGNFQNEESICCMAEHATIWVPTIVTVSNLLENGRYPAETLA
                     WIFETQKKGLQLAFEKDVVLAAGSDAGAYGVLHGKGIREEESVMRKILGAENGQELEK
                     RLAFGEKKLREKF"
     gene            complement(18014..18610)
                     /locus_tag="PLN77_01145"
     CDS             complement(18014..18610)
                     /locus_tag="PLN77_01145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM42918.1"
                     /translation="MIKIAMIRHGKTYGNTLGRYIGVTDEPLCEEGKKELEQRTMDTV
                     ELLFVSPLKRCLETADILYPYAKQVKIPEFAECNFGEFENKNYQELKDNPKYQEWIDS
                     MATLAFPGGESPQQFKDRCIAGFDKMVDTCIRRHYRNVAMVVHGGTIMSILEQYGVPQ
                     KSYYDWRVENGCGYHLYLSEQNWKMGRREAVVESEIRR"
     gene            complement(18603..19010)
                     /locus_tag="PLN77_01150"
     CDS             complement(18603..19010)
                     /locus_tag="PLN77_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRM42919.1"
                     /translation="MKLVIGGAFQGKLDFAKKMTGKETGWADGAAVSKEELSACSGVY
                     DFQKLVRRWLLLEEETGTAPEAELEELLQKNPEICIVTNELGYGVVPIEPFDRKWREK
                     TGRICTELAAKADSVTRVVCGIGILLKGEEKHD"
     gene            complement(19007..19783)
                     /locus_tag="PLN77_01155"
     CDS             complement(19007..19783)
                     /locus_tag="PLN77_01155"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRM42920.1"
                     /translation="MKTIWNNFKVAFAMYSKIPMPPADWEKENMKYALCFFPWVGLAV
                     GAVSAVLFWLLQQIGAGSMLRAAVLTAVPVLVTGGIHLDGYLDTMDALSSWREKQRRL
                     EILKDPHAGAFAIIMGCLYFVLYAGAAGELVWKIFPAYAFGFAVSRSFSGLSIAFFPN
                     ANPKGTAAAFGMSAEKKRTAAVLVVTIFAVTALSASFCVWAALDVLVVSALVFCYYKW
                     KSSKYFGGITGDLAGYFLSLCELAQLLSLVVLSLGMENWK"
     gene            complement(19785..20306)
                     /locus_tag="PLN77_01160"
     CDS             complement(19785..20306)
                     /locus_tag="PLN77_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRM42921.1"
                     /translation="MIHLVTGGSGSGKSAYAEQCILDFGGTRRVYIATMQPFGAEGQA
                     RIARHRKMRAAKQFSTIECYTNLKEVELEPGSDVLLECMSNLTANEIFDPSGAGKARA
                     EEEILAGVSRLAHQARNLVLVTNEIFSDGYRYEEETMEYQRILGNINVRLAEMADCVT
                     EVVYGIPVRIKKE"
     gene            complement(20324..21373)
                     /gene="cobT"
                     /locus_tag="PLN77_01165"
     CDS             complement(20324..21373)
                     /gene="cobT"
                     /locus_tag="PLN77_01165"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRM42922.1"
                     /translation="MTLQETIEQIHPLSKEAMDRAKAHWDGIAKPLHSLGKLEDVLIQ
                     IAGITGKEEISIEKRALLTFCADNGVVEENVTQSGQEVTATVAENFLQEKATAAILCR
                     GAHADLFPIDIGMARDTKVPRYKVAYGTKNMAKGPAMTREEAVRALETGIAVAEEKIA
                     AGYQLMATGEMGIGNTTTSSAMAAVFLNQPVPELTGRGAGLSSEGLARKIAAIEKAIA
                     INQPDPSDPIDVLAKVGGFDIAGMAGVFLAGAAHGVPVVMDGFISCVAALLAARIAPF
                     AKDYMIASHVSKEPAAHLLLAELGKEAFLHCDMCLGEGTGAIMLFPILDQANAVYRGM
                     ATFDEAQIETYVPLT"
     gene            complement(21409..23640)
                     /locus_tag="PLN77_01170"
     CDS             complement(21409..23640)
                     /locus_tag="PLN77_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRM42923.1"
                     /translation="MKNKVIRGVLAAACVTTAVSAANVFGAGTEQSLVNEASAVTQEE
                     TEETSEAETTDENTPEMTETETPDSTAENAASDLPAAEVQSGKPEETAVSVQAGSYYP
                     WVNENGIWYFKDPDGTIVKGAWREYDKNRYYLNNDGKMAVGWKKLDGAWYYFQSWGGV
                     YRDAFYTVKNVPYYSDADGKMATGWKLIDDVYYYFDDQGAMYRNRFFEYDKNTYYVDA
                     DGKMASGFEQIDGIWYYFRSWGGMAQNTFLTHKNNIYHVDTDGKMTTGWLLQDGTWYY
                     FRSWGGMYRSTFFKAPTGSALYYADENGKMAVGKKQIDGDWYYFKDWGGMYQNAFIKN
                     GTSVCHAAADGKLTVGWLQQGSTYYYFDETGEQYFDRFFEYDNNTYRVNADGKMVTGW
                     QKINGTYYYFRGWGGMYRSTFFQLGGETYYADADGKMVTGWLSKENQWYYFRENGAMY
                     RNTFFTHLNNSYYADANGVMVTGERTINGASYYFKDWGGMAKNQWLNAQKRMVSGDPQ
                     TGWYYFGSDGKMVKSYYALLKKNSSNWYYSFDENGVCILGSSQYVRAKDSVSGKYYTM
                     EHQYYTDPSVSDRDFFAAICSAEAGVQRKTGMTAVAMVIRNRMAAQNISLRTAIYKQQ
                     QFEPARNGSLTNYLTGIAEQSSSIINQLKNNGAYGAVDESQSIMDAYLKNGTKRVIPG
                     FGDTRDDFDYLYFMTPKAFKNLNMDKEKCVTYTYTYKWTTSSGTAREDSHIFFVNWVK
                     KQS"
     gene            complement(23722..25206)
                     /gene="thrC"
                     /locus_tag="PLN77_01175"
     CDS             complement(23722..25206)
                     /gene="thrC"
                     /locus_tag="PLN77_01175"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRM42924.1"
                     /translation="MEVLYKSTRGNGETVTASQAILKGLSDDGGLFVPTSIPALDVPM
                     EKLAAMSYQEVAYEVMSRFLTDFTEDELKNCIANAYDAKFDTEEIAPLTKADGVYYLE
                     LFHGATIAFKDMALSILPHLMTTAAKKNGVKNEIVILTATSGDTGKAAMAGFADVPGT
                     RIIVFYPKNGVSPIQEKQMVTQKGKNTHVVAIHGNFDDAQTGVKKMFNDKELGKELAD
                     KGFQFSSANSINIGRLVPQVVYYVYAYANLVKNGEIADGEKINVTVPTGNFGNILAAF
                     YAKQMGLPIAKLICASNENKVLFDFFRTGIYDRNRDFILTTSPSMDILISSNLERLIY
                     RIAGNSEAADRELMQDLAKDGKYEITDAMKKELADFYGNYASEQETADAIAKLYSDTG
                     YVIDPHTAVAAAVYGKYREETYDETKTVIASTASPYKFTSSVLAAIAPEYDTLPVLEQ
                     VDKLSALANTEIPRAIEEIRTAPVLHTTECDVDQMPETVRAFLK"
     gene            complement(25416..26021)
                     /locus_tag="PLN77_01180"
     CDS             complement(25416..26021)
                     /locus_tag="PLN77_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42925.1"
                     /translation="MLFKNKIEYTKGMFVEIYGTEIKKVRLVLRIITGIAVVAAIAFM
                     IYGAAARGFIMPGDFFNLGISILMALLCTFLPNLMARSQMKKCKKRGLLGERTLRFTE
                     QVLTMTYEKEGRSTDIPLEELTKVTEFDNFIRITIGGRSTFLDKKRFEIGDAAAFVTW
                     AEDKIAENAEKEEEALALEETEREAPALEDGEAKTEESDEN"
     gene            complement(26037..27698)
                     /locus_tag="PLN77_01185"
     CDS             complement(26037..27698)
                     /locus_tag="PLN77_01185"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRM42926.1"
                     /translation="MMNVKKYQKQYYMPPVPCMDWAEKDSIDKAPVWCSVDLRDGNQA
                     LVIPMSLEQKVEFFKLLVEVGFKEIEVGFPAASETEYEFLRTLIEHDLIPDDVTVQVL
                     TQAREHIIRKTFEALKGCKNAIVHVYNSTSYAQRQQVFKKSKEDILKIAVEGAKLLKE
                     LTEEAGEKYRFEYSPESFTGTEPEYALEVCNAVLDVWQPTADRKAIINLPSTVELSMP
                     HVYASQVEYMSKNLKYRDNVVLSLHPHNDRGCGVSDAEMGILAGADRIEGTLFGNGER
                     TGNVDIITVGMNMYMQGVDPELDFSDMPKLCHAFESFTGMSINPRSPYCGSLVFAAFS
                     GSHQDAIAKGMHWIEEKAPDTWTVPYLPIDPTDIGRNYDADVIRINSQSGKGGVGYIL
                     ETKYGLNLPAKMREAMSYTAKSVSDHLHKELRPDEIFELFKDTFENVTTPYNINGVHF
                     KQLNEGRIEAQVTSNYEGGEYATVYAEGNGRLNAVSNALKQQYNLQYDLVSYTEHALE
                     QSSSAQAIAYVGIKKPDGILSWGAGVDPDIIRASIDALVTAINNR"
     misc_binding    complement(27799..28062)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28188..>28748)
                     /locus_tag="PLN77_01190"
     CDS             complement(28188..>28748)
                     /locus_tag="PLN77_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42927.1"
                     /translation="AWCYFGITNVCSDPERAYAIFDDMATPENWIHKKFGVEGRDYKD
                     NGDGTYEIINPGDGAVNTEQNLGINLFQDLFARKDAANIANTEETTELFEKVKENSRD
                     AYAKTIEKKNPDAYTVNNDLGTDIGDAMKEYTWGVIGGGKSLDDWDSYVDKINGLGLQ
                     DVLDELKEIHGKQVEDYQKYLEEYNK"
     assembly_gap    28749..28758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28759..>29772)
                     /locus_tag="PLN77_01195"
     CDS             complement(<28759..>29772)
                     /locus_tag="PLN77_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM42928.1"
                     /translation="MKRRMMALLLAGVMGVGMMSGCGSSKSADSGKVVREESDEPIDV
                     LNQDEKMKLSIVCLQGYTQPDSEMQKWMEDRYNLDIDIIALPGWSDATSKISLLMGDE
                     TQRPDIIWWWNMEADYTKWVDAGLLVDISPYMKKYTNMVDYYNSVDPGVMFYASGDNG
                     IYRIPGDVAEPACETLWIRKDWLDNLGLAVPTTLDELEDCLYKFTFDDPDGNGVDDTY
                     GWNGDGYDYRTFWPWIQGSGDGNGRMDFLRLEDGSYIYGPTTDDCKEWLGRVAKLYAD
                     GVIDPSIITDTDRDEEMANGGFGATYSWVSWNNPSSGTMQSFYASNPDAEWIPIDMVS
                     GDN"
BASE COUNT         6490 a         8599 c         6124 g         8539 t
ORIGIN      
        1 cagacgcaag aatcatcagc catgcgctga ttctcgccgg tatgtaattg acgatatcgt
       61 ccagctttgc cgcacaccgt ccaaaataca gatatttgtc atttttgtat cccaccatgg
      121 aatccatggt attgatggac ttatacagaa agcccactgc cggtccgccg atggcaagaa
      181 agaggagcgg tgcaatgaca ccgtccgacg tattctccgc gatcgtttcc acggcagctt
      241 ttgtcactcc tgtttccgaa agattttccg tatcacgtcc tacgatcatg gaaaccgcat
      301 agcgcgcccc cggaagatcg ttttctttta atttctgata gaccttcatg ctctcatcct
      361 tcaggcattt cgttgccgga atctgccagc accagagaac ctccaaaagg aagccgatga
      421 ccggatgaat tttaaatgcc gcaaagagca gcagtgccgg aacaccgtat cccgccagac
      481 agacgaggat cacgagaaaa actcctccgg caagctctcc ctttggcgtt ttcggaaata
      541 ccttccgcag ccatttttcc agcagggcaa tgagatttcc gatcacacgg atcggatggt
      601 acaaaaagga gggatctcca agcagcaggt ccaatccata gcccacaatc agggctgcta
      661 atgctgtttt catcttgttt tctcctgttc tttccggtaa gagacacact gctccagaaa
      721 ttccagtgcg aggtgcggac ttgccatata gtaaagatgc gggaatcccg ccatcatccg
      781 ttttgtcgca tgaatgcatt cccagttccg cttccgcagc ggttttgccg catgccatgc
      841 acttccgcag tctgtgctgt caaagtaatg gaactcatgg ccgcgcaccg gatttccgcc
      901 gttccgaaag gtatccttcg tgtttggcgt gagggtgata tagccgaatc ttcccagctt
      961 ttttgtccgg taaacatttc ccggaacagc ctgtaccata ggatacacat ttccatccat
     1021 atcctccatg gattctgaca ggtacatata accgccgcat tctgcaagaa ccggcattcc
     1081 ctgacggatg ttatcgcgga tcttcgttct catggtctgg ttttccgaaa gctcttctgc
     1141 gtgcagctcc gggtagccgc cgcagaacag aaatccgtct gtattctttg gcggctccgc
     1201 atcggaaagc ggcgaaaaat attcgatttt ggctccaagt gacgcgagaa gctccagatt
     1261 atctcgataa aagaaacaga atgcttcatc ctttgcaact gctattctta gatttctctt
     1321 tttcttttct ttgtaatgta tttcattctc tgaggcattt tctgcaatct taagaatccc
     1381 gtctatatcc agtgtttctt tcatctgact tgcnnnnnnn nnncggcaag aatcggcttc
     1441 gcagcacgca ccttggcggc tgcttcgctt tctgtcacct tacgcaggac ttccggcatt
     1501 tttcccgaga gctcttctgc cccttctgca atcgcaagaa ttttttcaaa atccagtgtc
     1561 ttctgcagtg tttctgcaag cagattgacc tgctcctgca gattttccac ctcatccggc
     1621 atcacaaggc cgaggtggcg gctttctaag tggcattcct tcagttccgg cacaaagccg
     1681 acacaggaaa tgccaagttc tttttccgcc atcggcgcga tctcccggta cagcatctct
     1741 gacatccggt taaagattac accggcgatc cggctgtcct ctttgtactg caggtatccc
     1801 ttgataaagg caagcgctga aacgctcatg cctttggcat cgacaaccag aagaaccggc
     1861 gtttttgtca taccggcgag atcgtaggcg ctgcctttgg tagaaattcc gccaagaccg
     1921 tcgtaatacc ccataacgcc ttccaatacg gaaatgtcat gacccgctgc atgtttgcag
     1981 aacagatact gtgttgtttc ttcctctgtg aaaaacggat ccagattaca cgacggggcg
     2041 ccgattacct gttcgtgaaa catcggatcg atataatccg ggccgcattt aaacgctgcg
     2101 ggctgctttc ccatctgctg cagtgcacgg agcagtccgc aggtgatcat cgttttcccg
     2161 cttccgctcc ttcctgctgc cagcatgatt cttggtgctt tcattctttg gcacctccct
     2221 gggctgtaaa gatataaacc ggattctggg caatcatcag atggcttctt ccggcctttt
     2281 tcgcattgga agcctgcagg cagacgattt ccggctcttc aaacgcaagt tttttgaaac
     2341 acgatgctgc ctccgctaat gtttccacgg ttactgtatt taataccacg cggatgttcg
     2401 gattcttccg caggagcagt tccagaatct cttttaagtt tccggcagac ccgccgataa
     2461 atgcatgcgt cggaaccggg agtgcttcga gtgcttccgg tgcagttccc tcaataattt
     2521 ccagatgatc cactgcaaat ttctttttat tttcctgcag aagtgcaacg gctgccggat
     2581 ttttttctat cgcatatacc tttccctgtg ctgcacgaag cgccatctca atcgaaacag
     2641 aacctgtgcc cgatccaata tcatagacaa cagaattcga agaaagctgc agatgtgaaa
     2701 gagacgcatt gcgcacttcc cgcttggtca tcggtacttt gtcacgcaga aatacttcat
     2761 ctgccatgcc ggctgtaaac tctggttccg gattcgggtt ttcaaacaga agaaccgcaa
     2821 gcggagcaaa ctccatttta gcaagttcga atggggttcc tgtcacaatc tgttcttccg
     2881 gatacgacag atccgttccc acatgcagca caatctgctc ccagccgtac gaacttaacg
     2941 tctggcagat ttccctgaca ctgccctttc cgccggcgat cgccagagtt ttccggtttc
     3001 ggcggaccgt ctccagaaag ttgcagtgct gtccgtgcag gctcacaaat gctgcatcct
     3061 cccaggacac tccgattttg gcacagaaat aaaccagcga agaaattccc ggctctacac
     3121 ggacagaaaa atccgcatct tccgcaagga cggcctgaag tttctttgct ccgctgtaaa
     3181 agccgacatc acccgactgc agaagaacaa tcttttcata ttccgggtgc gccttcagat
     3241 actcgtaaat cttttccggg ctgtattctg caagcattgg cttctgatat ttttctactg
     3301 cttttgtcat gcgtcccgca ccgatcagca catccgcctg ctcacacgcg tctacgagcg
     3361 ccagcgtcat ctgtgcttct gcaccaggtc cgattccggc gatcacgacg tcccgtttca
     3421 cggacagatc aaatttttca acgagatacc gcttgacctc gtcctccgag agtccttctt
     3481 cctctttcgg acggccgatc agaacgacac ggacaccggc cttttttgct gcctcaattt
     3541 tttcttcaaa gccgccgttt cttccggatt ccttcgttac catccattcg gcatggaacg
     3601 actgcagcat tgcaacgttc atgtccatag aaaacggtcc ctgcatgcct atggtatgtg
     3661 cggcatcaaa tccaagttcc gcgcaggatg caattacgcc tggcaacggc agcacccggg
     3721 catacacacg gtttttataa tccggaagct ctgcaaattt tgcgagttcc ttgcttcctg
     3781 ttgtcaccag aatctttccc tctgtgcccg ccagatacga taccgcttct tctgtcgtgt
     3841 cgaccatgac gcaggatgga tctgccgcaa gcgacggccg cagcagccga agatacgttt
     3901 ttcctgcttt ttcacatgca gaccggatat tttccgacac aagatcggca tacggatggg
     3961 ttgcatcgat aaccatttcc ggctgttctt cgccaaaaag ctgttccatc tcttcttctg
     4021 tcagtcttcc gcttcgaacc gtcagggcac cgccctcctc gatccggcac ttgccgtatt
     4081 ctgtcgccgt acaggcaagg gatgcaatct catatttctt cagaaaactg cagatttccc
     4141 ggccttccgt cgttccggca aagacgatta ctttattcat agcgatatcc cctcggcgtt
     4201 accatgtgat cccggatttc ctttgtctga ctgtttccga taaataccgt cgtaaacata
     4261 tccacttttg tattgcgaag ttctgcaagc gtcatggtcc ggctcgtctc cccgtctctt
     4321 ccgatctggc ttacgattcc gcagacggtt tccggcgctt tgtaacgaag catcagatca
     4381 cacgcttttt ccagataatc cgctctctta tggcttcccg ggttataaag acagatgaca
     4441 aaatccgctt ccgatgccag cagaagacgt ttttcaattt tctcccacgg tgtcagaaga
     4501 tcgctcaggc tgatcagggc gaagtcgtgg atcaacggtg caccaagaac ggcagcgcct
     4561 cccgttgcgg cggtcacgcc tggaattacc ttaatttcaa tatcaggcca ttcttccgaa
     4621 agctcataca tcagacctgc cataccgtaa acgccggcat ctccgctgca gatcatagca
     4681 acctgttttc cctctgccgc agtttcaaat gccagacggc agcgctccac ctctttccgc
     4741 atcggcgtcg tcagaaattc ttttcctgca aaatgttctt ttaccagatc cacatagacg
     4801 gtatagccga tgatcacatc gcagttccgc agcgcatccg ccgcctcgat cgtcattttt
     4861 tcatatgctc cggggccgat gcccacgaca aaaattgtac tcaaaatact gccctccagt
     4921 ccgccgcagc cagtgcaact gttacaccgt ccgctgcctt ttttttcttt atcagtctgc
     4981 cgccggcgag aactgcgctg cgctcacaga cattgtcaat tcctgtaatc ttcttcacaa
     5041 acggagacgg cgtaaattcc ccctccgctg tttccagttc ttctgcagaa aaggtccggt
     5101 actgccagcc atacttttcc accagctgca gaattcccgg ctcctgcgct ttcagatcaa
     5161 tacttgccgc ctgtcgaact gcttttggag aaatcccggc cgccgcaagt gccgcaagaa
     5221 ccttttcttc aatccggccg gccggtgtat tcttcttaca gccgatgccg acggtgatca
     5281 ccttcgggat caggtgaagt gttctctcat atggcgaatc ctcttccgat gtggtaatgc
     5341 agattcccat cgacggtttt gccgcctctt ccgggatcag ttcctccggt tttgttccat
     5401 ccaccggaaa acggctgcag aatccaaccg tttttccgtc caccagaagc gccgctgcct
     5461 cttttgccag ctccatctca caaatttcac aatgattttt tacagcaaag acatcgaccg
     5521 caaacttgtg attgacatcc gttgccgttg taatgaccgg aaccgcaccc gttacagctg
     5581 caacttcttc cgtcagctcg tttgctccgc cgatatggcc ggaaagtaat gaaatcacga
     5641 actttccctg ttcatccata cacagaaccg ccggatctgt ctttttactt ttcacgtacg
     5701 gtgcaatact gcgcaccgca atcccgcacg ccccgataaa caaaatcgca tccattttct
     5761 gaaacatgcg ctctgtccat gcaggaagcg acggtgcaat cgcaggaatt cccgcttctt
     5821 tcccgtactt ttccagtgca tatccttcac agctgtgttt tttcagtgcc gcacagagtt
     5881 tttgattcca gacgcatccg tttcttgtaa aacttgctat ggctattttc atcttatttt
     5941 tctgcctctc ggaattcggt ggtaaacgcc ggattgtaca gatcacttct gcggtaattt
     6001 ccctgggaaa cggcatttcc gatgatcatc agcgctgtct tggtgatctg gttctttttc
     6061 gccgtctcgg caagtgttcc aacggtgcag acgaacgtct tctcctccgg ccaggttgct
     6121 ttgtaaacga tcgctgccgg agtatctgcc gtatagccgc ctgcaatcag ctcacgggaa
     6181 agttcctcta agagtcccgt gcttaagaat acaaccatcg ttgcctgatg tgccgcaaaa
     6241 gacgcaatgc tctcccgctc cggaaccggt gttcttccgg ccattctggt gatgacaaca
     6301 ctctgggaga tatccggcag cgtatattcc atttcaaggg cagcagccgc tccgcagaaa
     6361 gagctgacac ccggacagtc atcgtgagcg attccaaggg catcgagcgc atcaatctgt
     6421 tcgcggattg caccgtacag gcacggatct cccgtgtgga gtcttgccac agtctggcct
     6481 ttgctttcgg cctctttcat aacatcaatc acctgctcca gcgtcatggt tgcactgtta
     6541 tataccacac agccctcttt tttcagatcc agcagtgccg gattgacaag agaacctgca
     6601 taaatgataa tgtccgcctc accaagcagc ttctgtcctc ttaccgtaat cagatccggg
     6661 gctccagggc ccgctccaat aaaatgtacc atctattttt tcctcttttc ttttacaata
     6721 atcagtgaat agtaacttgc ctggtccggg atttcttccg cgctgaaata cttctgttcc
     6781 gtatccatgc cgcagttttc taccatcacc gcccggcagt tcttttcctt caggaactgt
     6841 ttcacagccg gcatcttttt acccgctttc atcagaacct ttgttcctga aagttccagt
     6901 gcatcttcaa tctggtaaga cgccggaatc acatgcagtt cttcggaatt ttccaccagt
     6961 ccctcggaaa gagatgctgc tgccgcgcag aacgatgtaa caccgcttac aattctcgca
     7021 tcgtaaccgt cttccagtac ccgtctgtgt acataaaggt aggtagaata cacagttgga
     7081 tctccgagtg tcagaaatac tacgtttttt cctgcatcca gcagctcctc tattttttct
     7141 gcaccgcgcc ggtgattttc ctccagttct ttggcattct ttgtcatcgg cattggaata
     7201 gcaacgagct ctttttctgc cagttccggc actgcctgaa cagcaatctg gtaggcaatg
     7261 ctctcttccg gtttctctcc tggtactgca atcgtatcat tttcccggat cagacggacg
     7321 gcctttaacg tcataagctc cgggtctccc ggtccgattc ctgttccaaa taaaattcct
     7381 gtcattttgt ttttactctt ttctgttttc tttttcttcc tgctcccgca ggcgctccag
     7441 aagttctccc gctcccgggg tttccccgag caggccctca ttgcttgaaa aaacaatcgc
     7501 ctccgtctgc actctgccct caatatggta attcatgtaa aaagcaattt tttctgtcac
     7561 tttttccatg gtctctttca ggcatcctgc ttcctttaaa atctgcaccg cttcttccgt
     7621 cgttccggtc tccagcagtt tttttgccag tgcaagatcc gcccctgcac gcaccgcctg
     7681 cgccgtaaga agctccgcac ggcagtctgc ctcgtgggaa tgggtattca taattccgcc
     7741 ggacaccttg acaaattttc caagatgtgc cacaaaaagg atggcgtcaa agccgagctc
     7801 agctgcaaat tccagtgttt ttccgacgta attactgcac ttaacggaat ggtctgcacg
     7861 gacccacgga tacgttttta agaagtcctg tccgtaattt cccggtgtca ccagaaggac
     7921 gcggtcaccc tgtgctttcc gcatttccat ctcgacgcgg atcgtggaaa tcagcgcctc
     7981 ctcgctcatc ggcactacaa tcccgctcgt tccaaggatt gaaatgccgc cggtgatgcc
     8041 aagtctcgga ttgaatgtat gctttgccag ttcttcccct tccggtacgg agatgataac
     8101 ctgcagtgcg cggaaatcat cgagtttttt ctgaatctcc tccacattct cgcggatcat
     8161 ctggcgcggt acatggttga tcgccgcgtt tccgaccggc tgatccagcc ccggctttgt
     8221 cacacgcccg acgccgaatc ctccatcgat tacaattccc tcttctgttc caaacgtcac
     8281 ttccgcgtaa atcaatgctt tatctgtcac atccggatca tccccgccgt cttttcggac
     8341 ggcacagctt actttttcgg cacttctccg gatatccagc aattccagat tcagaacaat
     8401 gccctttggc gtatggattt tcacttcctg tatttctttc ccggaaagca gcataaacgc
     8461 cgctccccgc gctgccgctg cagcgcagga acccgtcgta tatcccatgc ggagtttctt
     8521 gctgccgcgc ataatatatg catcttctaa gcctgtcctg ctcatgtttt tctccttaac
     8581 agatccggcg gtttttctgc cggatcacgc tatctgtttt atcataacac acaatgaaaa
     8641 aatgctgaac cattttcgca tattttttca aaaaagatca atttttcccg gctgtttttc
     8701 tatctcagaa acttattctt ttcccagctt cctgttgttt caaactccgc ggctcttctt
     8761 tctgcctgtt ttacaatttt ttcatcaatt cccatatttc caagcagcgc aatcgcattc
     8821 tttcctcttg ccggcccctg ttccaggcga tacgggaagg aaatactctg cgcggtaatc
     8881 tgctcttcaa aatgataatt ggtgtagaga tcttccagca gatacgtcag ttcagtatca
     8941 tgtgttgctg caagacagag cacatttgca cggcgcaggg cagcaagcac actctctgac
     9001 gctgcaatcc gctccgtcgt gtttgttccc cgcagcacct catcaatgac gcacagaagc
     9061 ggtgtttttt cacggcttgc ctccagaatc cgttttaaag attttacttc cacaacaaag
     9121 taactctctc cctgtgccag attatcgcgg agtgccatgg atgtcatgat ccggcacaca
     9181 acaccgcgat accgcttcga aagactcgtg ttcagtgcct gtgccagaat cgcattcact
     9241 gccatgtttt tcataaatgt ggattttccc gacgcattcg agccggtcag cagaattccg
     9301 ccctccgcat acagactgtt tgccaccgga tgtgtcagaa gcggatgata aagctcctcc
     9361 gcatcgatcc gacagcccgc ctttttctca tccaggaatt ccggccggca gtaatacgaa
     9421 aggctctccc ggtacgatgc tgcagacagt gcaaagtcca ggagtccaag atcctctgcc
     9481 atctgcagaa tcgctttttc atacgatttc actccggaaa caaccgaaga atactcgatc
     9541 agatccagca gaaagaacgt gtgaaagtat gcaaaaatca tgtttgctgc accgccctgc
     9601 gattctttcg tattcacaag tgttctgcat tttcttgtaa tcggacggag ttgtttttcc
     9661 tgttcttcca gacgcttctg atacatttcc aatccggaaa ttttttcttt tccaagttcc
     9721 ctggctgtcc gctgcagccg caggatacag ccaaacgctt ttaatgcact ctccagcttt
     9781 ttgctctccc ttccatgcat cagtccgttg ccgaccatag aagcaaacag aacagccaca
     9841 cccagcggag gcagtacaaa cagcagtcca attccaagca cagcaatacc ggccatcaga
     9901 agatatttcc ccttctctcc cggctccgcc tccatcagta cggctatttc ttctgtcggc
     9961 ggattcatct tttcttttcc catcaaaaga agtgctttct gcactgcttc cctggctttt
    10021 tggttctctg caaaaagctt catcagccgt tcccgctctt ccctgatttc tgttgttgtg
    10081 accggcatcc gaagcgccgt gtacagatat tcttccccgc acaccgacac tgtgtggtcg
    10141 actgtgtcaa aaaccgcatc gagatccagg tcattccagg taatcgcgtc cacgcagaag
    10201 ttttcccctg cttttcgtct cagacacgct tccagttttt ctttcttttc ccgtgccgtc
    10261 cgtttctcct gcttttttcc ccatttcttc cgcagttgta ccagcttctt ttcctgttca
    10321 tttctgttat tctgtaccat ccaaactccc agcagtatca gaaccacaaa aattcctgct
    10381 gccgtgtaca gttctttcat gtcttatcgc catccttttt cctgctctgt atcataagtc
    10441 ttttgattct gtcttattct agcacagaac cctcttccct tccagatttt gttgcaaaaa
    10501 ccatctccac cgccgcatgt acagccagtc tctgtgaact gctgccgtca ccaatgcagg
    10561 tggaaagggt aagtatcttt tgaacttcct tttcctcacc gctgccaaag ataatgctgt
    10621 tttcctttct ttcctgtacc catgcattcc agtctgcatc gctccatgct cccagccggt
    10681 aaacatcact tttggcatcc gtctcctcca cactccaaat acggcactgc catgcttcac
    10741 ccggcaaata caggtacaga tacggaaatt cctgtttcca cgacgcttca cgatactttt
    10801 tcaatcctcc gaacatcgtt ccatcccgca tattatgccc atataaaatc gtgtgacgtt
    10861 cagaaaaatc ctccggtgta tcacacggtg caaaaatact gccgtaaatc cgctccctgc
    10921 aaaacgcatc gtgtgtcaga taatatgcgt tatcctgccc atgcgcaacc ggaacatcta
    10981 aggcgcagcc cggaatccag agccacgcgc agatttctct attttttgat cgaagtcctg
    11041 caaaatcaat tgtccgaaac agattttgat ctgtactgcc cggttcctgc tcctgcactt
    11101 cttcgctttt ttcgttttcc tctttgttct cttctttttc ttctctgaca tacatctcca
    11161 ccgcttcttc ctgttctttt cgaatctgcc ggcttacctg aatctgcggc agataccaga
    11221 aaaaaagcgc cgccaatgtc agaactgcaa tccctcttct ttttctgctg attttttctc
    11281 tctccatttt gttttcattc caccacccga accgatggtg gaatgttttt ccgttttgtt
    11341 attcaaccgg aatccggttt tttacaatac tgttgtgtcc ataatcatcc cagcggtatt
    11401 tctgattttc aaatacaacc tcactttctt tgggatgtcc gcaaagattt accgataccg
    11461 aaaagacgcg cgcaggatct tttccatatg ccggctcttc tagttttttc acggtaacat
    11521 tcttcgtacc tgtcacctgc ataagcgaat accgcagcac cttcttctcc tgtacccggt
    11581 aatcgtttcc tgccggaatt ccccggatct gtaccgactg ttccgtgtag ccgtcctgat
    11641 ctgtcgtttt ctccacctgt gtttttgtaa acgtaagata gcattggtat gtatgttctt
    11701 ttccatacag atcctttccc ttcacggaaa acagaaacgt tggatttcca tgcgcccatg
    11761 taatctcatc cgccttaatc ttctttgtaa tcgtcaaggt ttcacatgca agatccttca
    11821 tcgtcacggt ctgcacttcc tgttttgcct ccagcacaaa cgaaaccggt tctgccgcaa
    11881 ggtatccatc cggcgcggcc tcctccacaa ggacataaga acccggcttt aatttttcca
    11941 gttcatgcgg gccgcttcct cctgaaatcc agctgtccac acggtttcca tccatatctt
    12001 ttaataccag ctttgcgccg gaaattcgtt ttccgctctc tgcatctatt ttttcaattg
    12061 tcagatgtgt tccatcattt tcgaccgcca ccgacagttc cgatttaccg tcaatttttc
    12121 cgttttctac cacaaattcc cggcatgtgc tgtccagcag atagccctcc ggagatgctg
    12181 tctcctgata gtaccagaca ccgtccaaaa gtcccgtcag ctcaattttt ccatttgcat
    12241 ccgtcttttt tgtctcatca aaacttccat ctttcgtcca gatatgaaag gctgctccct
    12301 gaagcacttt tccgtctccc tgacttactt tctgcagaac aaaacggttc ttccagtttg
    12361 taaacgtcag tgtgatctgt gcgttttgtt ccccgatcgt tattttcttt tccgtcggat
    12421 ccagcacata tccatttgga gccgccgttt cccgcacaag atacgttccg tactccagcc
    12481 cgtctttgaa cgcctctcca ttttcatccg ttttcagcac agctgctacc gcctgatccg
    12541 gaaacagcac tgttccgttt accgttgtaa ttgctccgct cgcaatcacc tgatatgttg
    12601 caccagaaag tgctttccct gagttttctt ccgtcttttt tatcttcagt gagcctgttt
    12661 ttcttgtgtt ttctgcctcc agatggatcg tctgcatgcc ttccttgtca atggaaaatt
    12721 cctgctccca gcttcctgca taaccagctg gattctttgt ctccacaaca cggaattttc
    12781 cattttcact gtctgtatcc agaattccct ccagcagata agaacccgtt tttttcaccc
    12841 agttcatctt tttgaattca ctccaggtgt tgtttttcca gcggtacacg gtaaattctg
    12901 cacccgaaat agcttctttt gtctctgcat ccattttttt cactacaaga tctcctactt
    12961 tcgacagatg acagggaacg ttcgctgtgt aaccaaattc cgaagtattc cacggcacaa
    13021 cagttattcc cgtttcccgg gatgctccat gccagttgtt gtacgtactg gaagtatcgt
    13081 ttacaaaatt atccggccat tttttcggtg ctttcactcc gccgccagga ccagttaccg
    13141 cgtcgttagt gcctccatca tagaaaatca tcgtctggga atttccttca aaggtaaatg
    13201 taacccagcc ttccgaattt tccggagttg ttccaccgtc cggctcagag ccgcgtcttg
    13261 ccgaccagat cgcttctgtt ctggcatcgt agacgattct ggcatctgcc tccttcgtca
    13321 gatagatatg ttctgtctca tcagccagat aaaagccctg cttaaagtcc agatctgaaa
    13381 gtgtaaaata tcctttcaca ttcagcggat ttcctttact gtcgtaataa gcaaaccgta
    13441 ccttgatcca gagcagacct ttaatatcaa tgaccggctt attgtggtca atcgccacga
    13501 ctaccgcatc cacgtcctca taccctgatg gtacaaccgt gttctgccag tcttccagcc
    13561 agatccgcat cgttacattt tccccgtggc actctccaac attcgtatat tccgcataca
    13621 tttttccttt tttcgactcc agcggcgtga aggaacggag attactccat cccttcacat
    13681 cggccgccgg ccaccatgtg gaacagcatt tcgaatggct ctctccgttt ttgcagaaca
    13741 gattgtccca ggtacttcca tctgtacttc caaaacggat ttttgtctca cttccgatca
    13801 gcggggtaaa atcatatcgg ccgtcaataa cagagtccga tgatacaact tctccgattc
    13861 cggctttcga aagagaagga agactcgtaa gactcgaagc cagaacatct tcccgaaaac
    13921 aaaatcccaa caacagcaga attcctgcga gaaaccagaa gcttttcttt gctcttttca
    13981 ttctttcctc ctttcagacc ggcccgcggt aagaaacagg aaggctcctg tcacggcaat
    14041 tccaatgggg accgctgctg acggatcgcg cagacctgtt tttggcgcgc tgtgccagat
    14101 agaggggcct tcttcctgcg gcagatcttc tccttcgaca gaaaaaatcc attttacccc
    14161 gcttttccga agtgcccatt catttccaag ttctgacggc acacggattg aaaaacagat
    14221 ttctttccgt tcatctggac tgcatattcc aagacttttt gctgcccata gtccctcact
    14281 ctctaaatca ccttcataaa tcacgctgtc tccctctcgt atttccagtt tcaccttgga
    14341 aagcagttct ttttctgttt cactgagttc ttccggaagt tccgtccgca gaaaaaattc
    14401 tgcacggctt gctgtcgtgt tcaccagtgt gaacgtatct tcccattcat ctcccggcat
    14461 cagcactgga aagttccgaa agaaattgtc gggtgccaag atcaggttcc gcgctgctcc
    14521 ttcgtacgaa acggtgagga agctctgctg cgaaacctcc gaaacggtat tatccgcctc
    14581 gtggacacac tgttcaattt ccagttcttc tccccacgga gcttctgccg taaaatccgg
    14641 cgtcacattc gctgcctgta cggcctccgc ctggatttca acggacagca cctgtccctc
    14701 cgcctcactg gtccacgcag atggaatggt gattgtacgg aaaaaatcga cagattcctg
    14761 ctttcccagc actttgggat aataataata ctctcctgct ttcagccaga cgtcctcgat
    14821 tcccccaagg tcctcttccc ccagacggta atcctgcgtt tcttcctcat caaacacagg
    14881 ctttacccgg acatagcatg gttcagcata attggtgatc cgcggaattt ttgaaatcac
    14941 ctgggaaggc agtacaatgc cgttttccgg tccctcatac ttcttttctc catccccgtc
    15001 ttcctcatat tcccagattc caatatttac atctccgaca gctattttgt taatggtctc
    15061 aacactgctc cggtaatatg catacgttcc gctgattccc atcagaagac ttgccgccgc
    15121 acatccgatc ctgacgcgtt ttcctttcat gtctgaccac ctctctcata agcatcccat
    15181 gcctcccgat agccgtgaaa taccatattc cccttctgga tctgcaccga ttcctcatag
    15241 accgaaacct caagttcttt ctgttccatt tcttttgcaa cgcggattcc ggtaaacaat
    15301 tccgtcgtgc tttcgccttc tgaaaccggt tttttgtaat aatagtagtc atcttctcct
    15361 tttacccagt tttccgtatc aagtccctca atcgcttcaa ttggttcact gaatataagt
    15421 gccgcacgaa tatagcaggc aacggaacct tcatttctga tttttactct ctttaccaca
    15481 cttttccctg gcggtacggg cgtcggtgtc ggaaattctt catcgatagt acttgtatta
    15541 tgtccgaaca ccatacgatt ccagacactc tcgtgatctg taaaatacgc cgaagttccc
    15601 ggcagcagcc aaatggctgc cgccatcgta cacccggccg cacccgcaag gaaaaacagc
    15661 cgtttcgtct ttttcttcat cttttgtccc ccagaaaact gcttaaattc caggtgcttc
    15721 tgtctgttcc ggctccgact gcggtgccgt tacctgcccc ttctgcccat tattctgatt
    15781 tacatacttc tcccaggcct ttgctgcccg ctcggcagcc gtacttccct caccggtatt
    15841 cagcgcctgg attgcataat aattgatcgg aatatcgacc tctttttcat ccagctgccc
    15901 ttccacgata ttagctgctg tcacgctcgt aaataaagga tccgtcgttt tgccagctgc
    15961 gagcacttca ttgtagagat atgtatatac catcgaattg ttctgttttt tcttgtacat
    16021 cagcacccat tttttcgaaa gctgattcat cgaaaacagt tccgtatcga cggctgcccc
    16081 gccgtttaaa cggtttccgg atgtatctgc aacgatcact tttgcgattg gtatggaaac
    16141 atccatatag acgtaagaat catttttacc tttatttttg atacgcgggt ccttcggaat
    16201 cacctgggtc ggcacaatat gattggtctt tccgtccgga tccggtgcgt acgatggttc
    16261 atttccttcg atatctactt ttcccacaac aaactggttc gtcacagaat ccttatccgt
    16321 cagatacgcc agtgttccgc ccgctgccag ggctcctgtc agaagcagtg atgctgccca
    16381 gaatacgatt ttccgctgtt ttttcataga tcaattcctc ctgttttcct aaataatttt
    16441 gctaattctt tttataactt gttttcctgc atgatttttc taccgtgcca aagctgccgg
    16501 attgtaaaaa gcatcaccgc tcccacggcc agaaaaaaga gtgttcctct ctgccggagc
    16561 cagatgatac caaagccaag ccagggaaga gctacgatca cattcccgat cacctgtttt
    16621 tccaaaacga tgcctgcatc ttcctccgcg ttggcatccc ccttagtata gtatccttcc
    16681 tcgctctttt ccacgatccg gtgtgtcacc accatatttc ctctccggta ggtaatcaca
    16741 tcccccggat tccattcctt cttccgtcca tccacaacca caacagatcc cgtcgggatc
    16801 tccggttcca tggacccgga cagtacgaca taggcgcaga aatactgcat cagataccat
    16861 cccgccgcca gaaaaaggaa ggaaagaaag ccctgcagcc ccagttttcc ttttgctttc
    16921 atttgctccc ttccgtcgat gcggatatcc agactgtcat ttttgcacgc aaaaaacaga
    16981 ggatagcatt taccctctgt ctcagaatat ccctgtttca ttttctctaa agttcttgta
    17041 aaagtgattt aaacttacca cagtccgggc gaaaacgcaa gcattgtctg cccgtttaat
    17101 tctaaatttt ttctaaagcc tgaaacgcag aaaattttca gaacttttcc cggagctttt
    17161 tttctccaaa cgcaagccgc ttttccagct cctgcccatt ttctgcccca aggatttttc
    17221 gcattacact ctcttcttcc cggattcctt ttccgtgaag tacgccgtat gcgccggcat
    17281 cacttcccgc cgcaagcaca acgtcctttt caaatgcgag ctgcagacct tttttctgcg
    17341 tctcaaaaat ccacgccagc gtttcagccg gatatctgcc gttctccaga agattcgaaa
    17401 ccgtcacaat tgtcggcacc cagattgttg catgctctgc catacagcag atgctctctt
    17461 cattctgaaa gttcccgtgt tcaaccgagt ccacgcctgc ctctgccgca tccatcacag
    17521 cctgtgcgcc gttcacatgc gccatcacag aaagccccgc atcatgcgcc gcagctgtca
    17581 ttgcaaaaac ctcttccctg gaaagcggct cactggtcac atgccccttt gtcgcgaaat
    17641 ccaggattcc cgtcgtcatg atcttgataa aatctccgcc cgcacggcgt acttcacgca
    17701 caagcgtaca atactctttc cagtctgaaa atgcgcgtcc gacaattttt ccataacggc
    17761 ccgccctgtg aatcgcaaaa accggtgtgc ggtatgtgat cccgtactcc ggcgcaaggc
    17821 tccgtgcaag aacacccgcc ccgtacggat ctccgccgtc acgcacatag gaaatccctg
    17881 ccttctgata cgccgccaga tgttcccgga tcagatcctc ccgcgggccg ttttcatgcg
    17941 ctgccgccgc ctttttgtaa tccacaccgt ccataaaaat atgggcatgg cattctgctg
    18001 tcaccatttt tgtttaccgc cttatctccg attccaccac agcttctcta cggcccattt
    18061 tccagttctg ctccgagaga tacagatgat atccacagcc gttttctact cgccagtcat
    18121 agtaactctt ctgtggcacg ccgtactgct caaggatact catgatcgtg cctccgtgca
    18181 ccaccatcgc cacgttgcgg taatggcggc ggatacacgt atccaccatc ttgtcaaagc
    18241 cggcgatgca gcggtccttg aactgctgcg ggctctcacc gcccggaaag gcaagcgtcg
    18301 ccatggaatc aatccactcc tggtatttcg gattatcttt taattcctga taatttttgt
    18361 tctcaaactc accaaaattg cactctgcaa attccgggat tttgacctgt ttggcgtacg
    18421 gatacagaat gtctgccgtt tccagacacc gtttcagcgg gctgacgaac agaagctcaa
    18481 cggtatccat cgtccgctgt tccagctctt ttttcccttc ctcacacagt ggttcatccg
    18541 tcacgccgat ataccgtcct aatgtattgc cataggtttt tccatggcgg atcattgcaa
    18601 ttttaatcat gtttttcctc acctttcagc aggattccga ttccgcacac cacacgggtt
    18661 accgaatcag cctttgctgc cagctccgta cagatgcgcc ccgtcttttc ccgccatttc
    18721 cggtcgaagg gttcgatcgg aaccactccg taaccgagtt cattcgtaac aatgcagatt
    18781 tccggatttt tctgcagcag ttcttccagt tccgcttccg gtgcagtccc ggtctcctct
    18841 tccagaagga gccatctgcg gaccagtttc tgaaaatcat atacgccgga acatgccgac
    18901 agctcctctt tcgaaactgc tgccccatcg gcccatcctg tttcttttcc ggtcatcttc
    18961 tttgcaaagt caagctttcc ctgaaaagcc cctccaatta ctaatttcat ttccagttct
    19021 ccattccaag agaaagtacc acaagagaca ggagctgtgc cagctcacag agcgaaagga
    19081 aatagcctgc gagatctcct gtaattcctc caaaatactt actgcttttc catttataat
    19141 aacagaatac cagtgccgat acaaccagta catccagtgc cgcccagacg cagaacgaag
    19201 ctgagagcgc cgtcacggca aaaatcgtca caacaagcac cgccgccgtt ctttttttct
    19261 ctgcgctcat gccaaacgct gccgctgttc ctttcggatt ggcatttgga aaaaacgcga
    19321 tcgaaagacc gctgaacgat ctcgaaactg cgaagccaaa tgcatacgcc ggaaaaatct
    19381 tccagacaag ttctccggct gcgcccgcgt acagtacaaa atacagacat cccataataa
    19441 tcgcaaatgc tccggcgtgc gggtctttta agatttccag tcggcgctgt ttttctctcc
    19501 aggaacttaa cgcatccatc gtatccagat agccgtcgag gtgaatgccg cccgtcacca
    19561 gaaccggaac tgccgtcagc actgcagccc gcagcatgct tcccgcgcca atctgctgca
    19621 gcagccagaa aagcaccgcc gaaactgcac cgacagccag tccgacccac ggaaagaaac
    19681 acaacgcata tttcatgttc tctttttccc agtctgccgg cggcatcggg attttcgagt
    19741 acatggcaaa cgccacttta aagttattcc atatcgtttt catcttattc tttttttatt
    19801 ctgacgggaa tcccatacac aacctcggtc acacagtccg ccatctctgc cagacgtaca
    19861 ttaatgtttc ccaggatccg ctggtattcc atcgtctcct cctcatatcg gtatccgtcc
    19921 gaaaaaattt cattcgtcac cagaaccaga ttccgcgcct gatgggccag acgggaaacg
    19981 cccgcaagaa tttcctcctc cgcccgcgct tttcccgccc cggatggatc aaatatttca
    20041 tttgccgtga gattcgacat acattcgagc agcacatcgc ttcccggttc cagctccact
    20101 tcttttaaat tcgtataaca ctcaatcgtc gaaaactgct ttgccgcgcg catcttccgg
    20161 tggcgtgcaa tacgcgcctg cccttccgca ccgaatggct gcatcgttgc gatataaacc
    20221 ctccgtgttc cgccaaaatc cagaatacac tgctccgcat acgccgattt tccgctgccg
    20281 ctgccgcctg taaccagatg tatcatcgtt tcacctgcct tttttaagtc agcggcacat
    20341 acgtttcgat ctgtgcctcg tcaaatgtcg ccatgccgcg gtatacggca tttgcctgat
    20401 ccagaatcgg gaaaagcatg atggcgcccg ttccctcgcc cagacacata tcgcagtgca
    20461 ggaaggcttc ttttccaagc tctgcaagca gcagatgcgc cgccggctcc ttcgatacgt
    20521 gcgaagcgat catatagtcc tttgcaaacg gtgcaatccg tgcagcaaga agcgctgcca
    20581 cacaggaaat aaagccgtcc atcacaaccg gaacgccatg tgcagcccct gcaagaaaga
    20641 cacccgccat gccggcaatg tcaaaaccgc cgacctttgc cagcacatcg atgggatcgg
    20701 acggatccgg ctgattgatg gcaattgctt tttcaatcgc tgcaattttc cgcgcaagac
    20761 cttcactcga aagcccggcg ccccgtcccg tcagttccgg aaccggctga ttcaggaaaa
    20821 cggctgccat tgcactgctc gtcgtcgtat ttccaatgcc catctcaccg gttgccatca
    20881 gctgatagcc cgccgcaatt ttttcttccg ccacggcgat tcctgtttca agcgcacgaa
    20941 cggcctcttc ccttgtcatc gccggacctt ttgccatatt ttttgttcca tacgcaactt
    21001 tataccgcgg aaccttcgta tcgcgcgcca ttccgatatc aatcggaaac agatccgcat
    21061 gcgcaccgcg gcagagaatg gctgctgtcg ccttctcctg cagaaaattt tccgcaactg
    21121 tcgctgtcac ttcctgaccg ctctgcgtca cgttttcttc cacaacgcca ttgtccgcac
    21181 agaacgtcag aagtgctctt ttttcaatcg aaatctcctc ttttccggtg attccggcaa
    21241 tctggatgag cacgtcctcc agctttccga ggctgtggag cggctttgca atgccgtccc
    21301 agtgagcctt ggcacgatcc atcgcctctt tcgaaagcgg atggatctgt tcgattgttt
    21361 cctgtaatgt catcttttgt tcgccccttc tttcgatatg acgtccgatc agctctgttt
    21421 tttcacccag ttcacaaaga aaatatggga atcttcccgc gctgttccgc tggaagttgt
    21481 ccatttatag gtgtatgtat atgtcacaca tttttcttta tccatattca gatttttaaa
    21541 tgctttcgga gtcatgaaat atagataatc aaaatcatct ctcgtatcgc caaagcccgg
    21601 gatcacacgt tttgtgccgt tcttcagata tgcatccatg atcgactggg attcatccac
    21661 tgcaccgtag gctccattat ttttcagctg attgataatc gaactgctct gctccgcaat
    21721 tcccgtcaga tagtttgtca gacttccgtt ccgcgccggt tcaaactgct gctgtttata
    21781 aatcgcggtg cggagcgaaa tattctgtgc cgccatccgg ttccggatga ccatcgcaac
    21841 tgccgtcata ccggtttttc tctggacacc cgcctccgca ctgcagatag ccgcaaagaa
    21901 atcacggtcg ctcacagacg gatccgtgta atactgatgc tccatcgtat aatattttcc
    21961 gcttacgctg tcctttgcac gcacatactg gctcgagccg agaatgcaca ctccgttttc
    22021 gtcaaagctg tagtaccagt tgctgctgtt ctttttcagc agagcgtaat acgattttac
    22081 catctttccg tcgcttccaa agtaatacca gcccgtctgc ggatcaccgc ttaccatccg
    22141 tttctgtgca ttgagccact ggttctttgc catgcctccc cagtctttaa agtaatagga
    22201 agcgccgtta atagtccgct ctcccgtcac catcacgccg ttggcatcgg catagtaact
    22261 gttgtttaaa tgcgtaaaga aggtattgcg gtacatcgca ccattttccc ggaagtaata
    22321 ccactggttc tctttcgaaa gccagcccgt caccatcttt ccatccgcat cggcataata
    22381 cgtttcaccg ccaagctgga aaaaggtact acggtacatg ccgccccagc cccggaaata
    22441 ataatacgta ccattgattt tctgccagcc ggttaccatc tttccatctg cattcacacg
    22501 atatgtattg ttgtcatatt caaaaaagcg gtcaaaatac tgctctccgg tttcatcaaa
    22561 atagtaatat gtactgccct gctgcagcca gccgacggtc agttttccat ctgcagccgc
    22621 atggcagaca ctcgttccgt ttttgatgaa tgcattctga tacatgccgc cccagtcctt
    22681 gaaatagtac cagtcaccgt cgatctgttt ttttccgaca gccatctttc cgttctcatc
    22741 ggcatagtaa agtgcacttc ctgtcggtgc tttgaaaaag gtgctgcggt acatgccgcc
    22801 ccagcttcgg aaataatacc aggttccatc ctgcagcagc cagccggtcg tcatctttcc
    22861 atccgtatca acatgataaa tattattttt atgcgtcaga aaggtgtttt gtgccatgcc
    22921 gccccagctt cggaagtaat accagatccc gtcaatctgt tcgaacccgg atgccatttt
    22981 tccatccgca tcaacataat acgtattctt gtcatactca aaaaaacgat tccggtacat
    23041 cgcgccctga tcatcaaaat aataatacac atcatcgatc agtttccatc cggtcgccat
    23101 ttttccgtcc gcatcagaat aatacggtac atttttcacg gtgtaaaagg catcccggta
    23161 aacaccgccc cagctttgga aataatacca ggctccatca agttttttcc agccgaccgc
    23221 catttttccg tcattattca ggtagtaacg gttcttgtcg tactctctcc aggctccttt
    23281 gacaatggta ccatccggat ccttaaaata ccagatcccg ttctcattca cccatgggta
    23341 atagcttcca gcctgcacgg ataccgccgt ctcttcgggc tttccgctct gtacttctgc
    23401 cgccggaaga tccgaggcgg cattttccgc tgtcgaatcc ggcgtctccg tttctgtcat
    23461 ctccggcgta ttttcgtctg tggtttccgc ctccgatgtt tcttctgttt cttcctgtgt
    23521 aacagcagaa gcctcattta ccagactctg ctctgtgcct gctccaaata cgtttgcagc
    23581 actgacagcg gttgtcacgc aggctgccgc cagtactccc ctgattactt tatttttcat
    23641 tatttattct tctctccctg caaaaacagg caaagcctgc tgtgtgctct gcctgtttta
    23701 aaattctgct cctgctttat cttatttcaa aaacgcccga acagtctccg gcatctgatc
    23761 tacatcacac tctgtcgtat gaagcaccgg tgcagtacgg atttcttcga tcgcacgcgg
    23821 aatctcggta ttggcaagtg cagacagctt gtctacctgc tccagtaccg gaagggtgtc
    23881 atactccggt gcaatcgctg caagaacgct gcttgtaaat ttatacgggc ttgcggtaga
    23941 tgcgatgacg gtcttggttt cgtcataggt ttcctcgcgg tattttccgt atacagccgc
    24001 tgcaaccgcg gtatggggat caatcacata tccggtatcg ctgtacagtt ttgcaatcgc
    24061 gtctgctgtc tcctgttcgg atgcataatt gccgtagaag tctgccagct cttttttcat
    24121 cgcatcggtg atctcatatt ttccgtcctt tgcaagatcc tgcatcagct cgcggtctgc
    24181 tgcttcgctg tttccggcga tgcggtagat cagacgctcc agattgctcg agatcaggat
    24241 atccatggac ggtgatgttg tcagaataaa gtctctgttt ctgtcgtaaa tgccggtgcg
    24301 gaagaagtca aacagaactt tgttctcatt ggaagcgcag atcagttttg cgatcggcag
    24361 tcccatctgt tttgcataga atgcagcgag aatatttccg aagtttccgg tcggaacggt
    24421 tacattgatc ttttctccgt ctgcaatctc tccgtttttc accaggtttg cgtaagcata
    24481 tacgtagtag acgacctgcg gaacgagtct tccgatgttg atcgagtttg cagagctgaa
    24541 ctggaaacct ttatctgcca gctcttttcc aagctcttta tcgttaaaca ttttcttcac
    24601 gccggtctgt gcgtcgtcga agtttccgtg gatggcaaca acgtgggtat ttttgccctt
    24661 ctgtgttacc atctgttttt cctgaatcgg gcttacgccg ttcttcgggt aaaatacaat
    24721 aattctggtg cccggcacat ctgcaaagcc tgccattgct gcttttccgg tatctcccga
    24781 tgtagcagtt aagatgacga tctcattttt cacgccgttt ttctttgcgg cggtcgtcat
    24841 cagatgcggc agaatggaaa gtgccatgtc cttgaacgcg atcgttgcgc cgtggaacag
    24901 ctccagatag taaacaccgt ctgcttttgt cagcggtgca atttcctctg tgtcaaattt
    24961 tgcatcgtat gcgtttgcaa tacagttttt cagttcatct tctgtaaagt cggtaagaaa
    25021 acggctcatt acctcgtatg ccacttcctg atagctcata gccgcaagtt tttccatggg
    25081 aacatccagt gctggaatgg aggtcggtac aaataatccg ccgtcgtcgg acagaccctt
    25141 taagattgcc tgggatgcag ttacggtttc tccattgcct ctggtacttt tgtataatac
    25201 ttccatgctc tttctctcct tcactttgac tcccggcaga gcctctctaa ctcccggccg
    25261 gatacgtttc agattagttt tacctgtacg cttcctctta ttataatgga tgaagtatga
    25321 aaaaacaaca aaaatataca aaaaatgact gtgcacaaaa atgcacagcc attttccacc
    25381 attctgccaa aatatttttt gtcagaatgt atgatttaat tttcgtcgga ctcctccgtt
    25441 tttgcttctc cgtcttctaa cgccggagct tccctttctg tctcctcgag tgcgagtgcc
    25501 tcttcctctt tttccgcatt ctctgcgatt ttatcttccg cccaggtcac aaacgctgcc
    25561 gcgtctccga tttcaaagcg tttcttatcg agaaaggtgc ttctgcctcc gatggtgatg
    25621 cggataaagt tgtcaaactc cgtcaccttt gtcagctctt cgagcggaat atcagtgctt
    25681 ctgccctctt tttcgtatgt cattgtgagc acctgctcag taaaacgaag ggtacgttct
    25741 ccaaggagtc ctctcttttt gcattttttc atctgtgaac gtgccatcag attcggcaga
    25801 aacgtacaca gaagtgccat cagaatggag attccaagat tgaagaagtc tcccggcatg
    25861 ataaatcccc gcgctgctgc accgtagatc ataaaagcga tcgcagccac aacagcaatc
    25921 cctgtaatga tccgcagtac cagacgtact ttttttatct cggtgccgta aatctccaca
    25981 aacattcctt tggtgtattc aattttgttt ttaaacagca tatgctctct cttttcttat
    26041 cggttgttga ttgcagttac cagcgcgtcg atggaagcgc ggatgatatc cggatctacg
    26101 cctgcgcccc aggaaaggat tccgtccggc tttttgatgc cgacgtaggc aattgcctgt
    26161 gcacttgagc tctgctccag cgcgtgctcc gtatagctga ccagatcata ctgcagattg
    26221 tactgctgtt tcagcgcgtt gctgacagca ttcagacggc cgtttccttc tgcatataca
    26281 gttgcatact caccgccctc gtaattggag gtaacctgtg cttcaattct gccctcattg
    26341 agctgtttaa agtgaacgcc gttgatgttg tacggtgtcg ttacgttctc gaaggtatct
    26401 ttaaagagtt caaagatctc atccggacgc agctctttat gcagatgatc ggaaacggat
    26461 tttgcggtat agctcatagc ctcgcgcatc ttcgccggca gattcaggcc gtatttcgtc
    26521 tccagaatat agccgacacc gccctttcca gactggctgt tgatgcggat gacgtctgca
    26581 tcgtagtttc ttccgatatc ggtcggatcg atcggcagat acggaacggt ccaggtatcc
    26641 ggtgctttct cctcgatcca gtgcatgcct tttgcgatcg catcctgatg ggaaccggag
    26701 aaggctgcaa atacgagaga tccgcaatac gggcttctcg ggttgatgct cataccggtg
    26761 aaggattcaa atgcatggca gagtttcggc atatcggaga agtcaagctc cggatcgacg
    26821 ccctgcatgt acatgttcat gccgaccgtg ataatatcca cgtttccggt tctctcgccg
    26881 tttccgaaca gcgttccctc gatacggtct gctccggcaa ggatacccat ctctgcatcg
    26941 gaaacgccgc agccgcggtc attgtgcgga tgaagggaaa gaacgacatt atcgcggtat
    27001 ttcaggtttt tgctcatgta ttccacctgg cttgcataaa cgtgcggcat ggaaagctcc
    27061 acggtgctcg gcaggttgat gatggctttg cggtctgcag tcggctgcca tacgtcgaga
    27121 acggcgttgc acacctcaag cgcatattcc ggctcggttc cggtaaagct ctccgggctg
    27181 tactcgaagc ggtatttctc gccggcctct tctgtcagct cttttaagag ctttgctccc
    27241 tcaacggcga ttttcaggat gtcttctttc gattttttga agacctgctg acgctgtgca
    27301 taggaagtgg aattgtaaac gtgaacgatg gcattcttgc agccctttaa tgcctcgaag
    27361 gtcttgcgga tgatatgctc tctcgcctgg gtcagaacct gaacggttac gtcatccggg
    27421 atcagatcat gctcgatcag ggtgcgaagg aattcgtact cggtctcaga tgctgccggg
    27481 aagccgacct cgatctcttt gaaaccaacc tcgacgagga gtttgaagaa ttctaccttc
    27541 tgctccaggc tcatcggaat tacaagtgcc tggtttccgt cacgaaggtc aacgctgcac
    27601 cataccggtg ctttgtcaat gctgtctttt tctgcccagt ccatgcacgg aaccggcggc
    27661 atataatact gtttctgata cttctttaca ttcatcatga ttttttcctc ccagaaagtg
    27721 aaatacaatt tattctgccg acataaattc gggatgaaag cataaaaaaa tctctcatct
    27781 tctgtgtcgg cttctgacac aagagacgaa agacttgcta tcttacgcgg taccactcct
    27841 gttttacaga gattttctct gtacactctg cggatacgga tgaaacatct gcatatcctg
    27901 cccttctaac ggtgggtttc cgtctgcacc tactcttttt attctaaatt tgggagcagc
    27961 ggctctgagg tcagttccac ggaattcgtc tgctgtttca catcacccaa cagctttctg
    28021 gattcggaag ctccatgtag tattcctctt ctttgccttt ttcctaactt ggctcttatg
    28081 ttaccatttt tctccatctc tgtcaagcgg aaaacgcagg attccgtaaa aatacacccg
    28141 ccggaaagga ggctccggcg agtgctctaa cgcatgattt cgttgtttta tttgttgtac
    28201 tcttccaggt atttctggta atcctctacc tgttttccgt ggatttcctt gagctcatcc
    28261 agcacatcct gcagaccgag cccattgatt ttatcaacgt aggaatccca gtcatccaga
    28321 gattttccgc cgccgatgac accccaggtg tattctttca tcgcgtcacc gatatcggtt
    28381 ccgagatcat tgtttacggt atatgcatcc gggttcttct tctcgatggt ctttgcataa
    28441 gcatcacggc tgttctcttt tactttttca aagagttcgg tcgtctcttc tgtattggca
    28501 atgttggcag catcttttct tgcgaagaga tcctggaaca ggttgatacc aaggttctgc
    28561 tcggtgttga ctgcaccatc gcccgggttg atgatttcgt aggttccgtc gccgttgtct
    28621 ttgtagtctc ttccctcaac gccgaatttc ttgtgaatcc agttctccgg tgttgccatg
    28681 tcatcaaaga tcgcatacgc tctttccgga tcggagcaaa cgtttgtgat accgaagtag
    28741 caccatgcnn nnnnnnnncg ttgtcgccgc ttaccatatc gatcggaatc cactctgcat
    28801 ccgggttgga agcatagaag gactgcatcg taccgctgga cgggttgttc caggataccc
    28861 agctgtaggt tgcgccgaat ccgccgtttg ccatctcctc atcacggtca gtatcggtga
    28921 tgatgctcgg atcgatgacg ccgtctgcgt acagttttgc tacgcgtccc agccactctt
    28981 tgcagtcatc ggtggtcggt ccgtagatgt aggaaccgtc ttccagacga aggaagtcca
    29041 tacgtccgtt tccatcgccg cttccctgga tccatggcca gaaagtacgg taatcgtatc
    29101 catcaccatt ccatccgtag gtgtcatcca caccgtttcc gtccggatcg tcgaaggtaa
    29161 atttgtacag gcagtcttcc agctcatcca gtgtagtcgg aacagccagt cctaagttgt
    29221 ccagccagtc tttccggatc cacagagtct cacatgccgg ctctgcaacg tcgcccggga
    29281 tacggtaaat tccgttgtct ccggatgcgt agaacataac gccaggatct acgctgttgt
    29341 agtagtcaac catgttggta tatttcttca tatacggaga aatatccacc agaaggcctg
    29401 cgtctaccca tttcgtatag tcagcttcca tgttccacca ccagatgatg tccggtcgct
    29461 gtgtctcatc gcccatcaga agggaaattt tggatgttgc atccgaccag cccggcagtg
    29521 cgatgatatc gatatccagg ttgtagcggt cctccatcca tttctgcatt tcgctgtccg
    29581 gctgggtata accctgcagg cacacgatgg aaagtttcat tttctcatcc tgattcagca
    29641 cgtcgatcgg ttcatccgac tcttctctta cgacttttcc gctgtctgcg gatttgctgc
    29701 ttccgcagcc cgacatcatt cccactccca ttactcctgc aaggagcagt gccatcattc
    29761 ttctcttcat tt
//
LOCUS       DAOMUF010000007        29576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_196_length_29576_cov_4.277734, whole genome shotgun sequence.
ACCESSION   DAOMUF010000007 DAOMUF010000000
VERSION     DAOMUF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 29576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29576
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196_length_29576_cov_4.277734"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="PLN77_01200"
     CDS             complement(<1..102)
                     /locus_tag="PLN77_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM42929.1"
                     /translation="MAVFRIEKTRDYTVMSNYHLRDRLLSLKAKGLLS"
     gene            complement(562..2331)
                     /locus_tag="PLN77_01205"
     CDS             complement(562..2331)
                     /locus_tag="PLN77_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM42930.1"
                     /translation="MRNLWTKIKKCLQAEMLQKMWKEFLWIAQYARRYWKAMIFYTAL
                     GVFGTGVSLASSVISKDLVDIITGHQTGKLLQTFALMIGFSFGNTLISQASAYASTMI
                     NLKVDREIQNDIFAKILVTDWESLTAYHTGDLVTRWSSDASNISSGILNWLPNLVIYT
                     VKFISALAIVVHYDPTFAIFALLGIPFSALLSRPLLRRMTNNNQRSAQMSAKLYGFNQ
                     EAFSNIQTIKAFDMIRFYVDRLKNLQQEYIKMRLDFQKMSIATSLLMTLISYLVSYSC
                     YGWGIYRVWSGAISYGTMTMFLSLSGTLTGSVNSLVSLVPSAISLGISAGRLMDIVEM
                     PQEDYSADREVEQFAEKYRASGIGLAMEDLSYTYHTGTEVFSKASLEAWPHEIIALVG
                     PSGEGKTTMLRLILSLLRAQGGEAWIGAGEDREQRIALSPSTRKLFSYVPQGNTMFSG
                     TIAENMRNVKPEATDEEIVEALKLACAWEFVEKLPDGIESMVKERGGGFSEGQAQRLS
                     IARALLRKSPILLLDEATSALDVATERKVLKNIMTDTYPRTCIVTTHRPTVLGICTRV
                     YAIRGKKCEELGEKEILEMEKDF"
     gene            complement(2328..3593)
                     /locus_tag="PLN77_01210"
     CDS             complement(2328..3593)
                     /locus_tag="PLN77_01210"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRM42931.1"
                     /translation="MRIRRQEGYLLQKIGNTHYLLPYGQKIADQQRGMELNETARILW
                     EALETPKTMEELQQKMIRCYEVPEEEQEELKKDIQAFVKELLAFGAVRRELGSPDGTC
                     AGELKIAGISIAVYGKEGCIPKQFASFEKKRGKEEATEKTEGEAAKKTGEKEVEETVI
                     KTENRQDAADLTLELIEHVPESHQNGNILIRNKDLTVCAWEEGYVLWFPALKNIYEIW
                     MKADGSFACIYYRLPMTEEEQDSLFLAIRPVFLFLAQKKGMFVLHSASLLYLEKAWLF
                     SGPSGMGKSTHTALWKKLFDTPFLNGDLNLIGKEGDQFVVYGIPWCGTSEIFTVEKKE
                     LGGIVLLEKAPEDKIVSLTKEQKTLRVMQRMISPPWTAGLMKKNLAFAEEIANEKPVY
                     FLRCTKNDTAAEVMHHRITEDELAQEALK"
     gene            3793..4230
                     /locus_tag="PLN77_01215"
     CDS             3793..4230
                     /locus_tag="PLN77_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HRM42932.1"
                     /translation="MYTYDIEQLLKNGSAVKLKPQGYSMYPLFLPGRDEAVIEHCVPS
                     ELHRGDVILYRRVQGILVLHRICRITRDGFYLVGDNQTEVEGPLSPEQIHGKLTGVVR
                     NGKSFSVSHPIYRMLSSIWLFLRPVRPFFWSVTAFFRRFFQKH"
     gene            complement(4288..5811)
                     /locus_tag="PLN77_01220"
     CDS             complement(4288..5811)
                     /locus_tag="PLN77_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRM42933.1"
                     /translation="MRIKNLFRNSFFSMLSQFALLILGFFSQRAMNLYMGAELVGMNG
                     VISNVINILSVTELGISTAIVYHLYGALARKDEAEIASLMNLYRKAYHVFAVLVFAAG
                     MAVLPFIHLFMKNNSYSLSYIRVLYAMWLVRTVLSYLLTYRKSLLIADQKEYIASITG
                     LILNVVSYLAIILLVTWEQNYLLALGIGTVTEVVLNIWISWYVEQKYPFLRQRRKEKS
                     TSSLTKRIVRDLKSLFISRISEKMLYCTDNLIISGFIGVGTVGRYANYCMITQAISNI
                     LTILANTIQPTVGDYFTEKNRQRNYEILRQLTFGFFCIASVVSVGLFSLITPFVRDIW
                     LGPSYELGWGITVFCIVVLLVRIMGLPLSLVMGVTGLFEKEKNVGASSALLNLIVSLA
                     LVKPYGMAGVLLGTLLSYLLQILYRYIIYFRDFLEMPGEALRYAQDIGEYLAVIAGEV
                     LLMQKIVPLIYGAGGMLLFCVCFLLCIAIPLLLDLVIFGRGWRLQSIVTMVKGMRKA"
     gene            6008..7423
                     /locus_tag="PLN77_01225"
     CDS             6008..7423
                     /locus_tag="PLN77_01225"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HRM42934.1"
                     /translation="MKTNRKKSFSYLLFFLLLLVFLALFAFLGIRSFYRANYMPEPCT
                     ESADELSNPYIGFYHMYGYVLGSDSLSNLPAASDSDAKNRQGIVMLEVNLQNFSNEDL
                     SDSALSQLDTILSAWQRHGSQVILRFLYDWDGKAMETEPQSLEQILRHMDQTAEVVNR
                     YTDCVFLMQGIFVGNCGEMNNSHYMSDEDCTTLMHHLAEVTDPSVFLSVRTPVQRRKI
                     LDSSERPTKETAFDGSLSSRLGLFNDGMLGTANDTGTYGDTAASADTYRSAWVREDEL
                     SFQNELCNFVPNGGEVTLDNPLNDLAHAIQDLSRMHVSYLNSEHDPAVLDKWKAAAYK
                     DKASVFNGLSGYDYIERHLGYRYVIQDTALDSSDFQIRLENVGFSVCYRKLDLQLTLV
                     SSDGEVFSFPISSDSRFWIPGTTAELSISLPLARLTKGSYTVYFQMTDPVLQREILPA
                     NTFSHDTHGFSIGTLEISKLY"
     gene            complement(7415..8797)
                     /locus_tag="PLN77_01230"
     CDS             complement(7415..8797)
                     /locus_tag="PLN77_01230"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HRM42935.1"
                     /translation="MWREKIKSILSWQKGREAKGRAEKKEFPELRGKQDKKELFQKAI
                     LQESRKAIQNPGCGWYHLYTFNAAQTGEPLYIACEEEELVLLRIDIGAFRDKPISEPS
                     LEWIRRILAFFREKRKGMILRFSYDLEGKGLEKEPGSIRLVEEHMQQIGEVIREYADD
                     IFAVQGILIGNWGEMHGSRYLTPDAFRTLTDTMIKAVDGACPVAVRKPAQWRELTLGW
                     TEQEKKKLTLFNDGIFGSETDLGTYGTLSPETPGQKICSREEELEWQKNEMCGKFCGG
                     EAIWGSGAEPAPFVSAPKAMADLERMHISYLNSTYDLRRLEQWKAETVEDESGLEAIG
                     KRLGYRFVVEGVEFVSEREELQITLENKGFAELTEEADCYLELERNGTLQKKIQIATD
                     ARLWKSGEKTTLCQKIKLPAELAEPEEQKLYLALYRRRDGRTISFANQGEKQGRPFLG
                     IFKIDPIVLI"
     gene            complement(8742..9560)
                     /locus_tag="PLN77_01235"
     CDS             complement(8742..9560)
                     /locus_tag="PLN77_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM42936.1"
                     /translation="MRQKEVLLIIPAYNEEKNIEKVMKQLAQPEIADIVDVLVMNDAS
                     SDNTNWLAKEYGCAVVTHVFNLGYGSGLQLGYKYAIRRGYSYVIQMDADGQHDVCNIP
                     KIYQRLKEKDDDGRTPDIVLGSRFMKGSTEFEVSFAKKMAFVLFRKMLYLATGRKIAD
                     PTTGLQGLSRKAVLYYSKYNHFDDKYPDTNMITQMLLLDFKVVEIPAVMHARTSGKSM
                     HSGLKPIWYMMRMFYSVLAVIFRCKVLKMDAGAGRIDVEREDKKYPELAEGKRS"
     gene            complement(9574..10776)
                     /locus_tag="PLN77_01240"
     CDS             complement(9574..10776)
                     /locus_tag="PLN77_01240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM42937.1"
                     /translation="MKKILFVINTLGRAGAETALLELLRRLDAEEYEISLYVLLGQGE
                     MISQVPQRVHVKNKKICTASVLSGAGRRKLIQTSFLAFFRHGRTIKKMQYVIQNFFRM
                     KKTGNIQPDKLLWRVISDGADRFSETFDLAVAYLEGGSAYYVADHVNAKKKAAFVHIA
                     YGNSGYTREMDRDCYEKMDQIFTVSQETRRHFLEFYPEYEKKVQVFHNILDTDRIARE
                     AQLPGGFSDSYTGIRILTVGRLAYQKAYDIAIPAMKKIREDGYEARWYVLGEGSERNA
                     LEKQIAELGLQEDFQLPGAKENLFPYYAQCDLYVHATRFEGKSIAIQEAQTLGCAVIA
                     SDCAGNREQIVDGEDGLLCTLTPEGIAEKVEELLDDPKKRERLAAAAKQKMNGQTEEI
                     WKLLNLMK"
     gene            complement(10800..11183)
                     /locus_tag="PLN77_01245"
     CDS             complement(10800..11183)
                     /locus_tag="PLN77_01245"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HRM42938.1"
                     /translation="MSTAAIVKACVILMGIGIMIVTFVLHAKKKLTVNLAVTWELLGI
                     AAILSGAVPRFSGWSSRIGLGSLVVLLITALLVLWGVYQMTIQISSLLMKNQELAILV
                     SLLNQENERILRELEKLTGKNAREL"
     gene            complement(11184..12650)
                     /gene="pelG"
                     /locus_tag="PLN77_01250"
     CDS             complement(11184..12650)
                     /gene="pelG"
                     /locus_tag="PLN77_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HRM42939.1"
                     /translation="MAGIGVRLNRIFEKNTLTTNLVGFFYSTVVTVAPMFVIIINLIL
                     MEYLLDFASVGYVTRELFSCTILYTFIFSLLTASPFNAVLSRYMSDVIYEERYQDILP
                     CYHIGMLLNIGLSCLLGIPFCLWEHFVGKVPTAYVFCGFCGYISLVLVFYSMIYLSIC
                     KDYQRISQYFFFGMLVAFVLALILHFILKWETTWSMLFSLTIGFFVTATLENALIRRY
                     FVRNSNHYRPVLQYFRKYWKLIFTNFLYILGLYIHNFVFWTTDMRMEIAKSFVCNQPY
                     DMASCLAMFTNISATIIFIARVEMNFHEKYKLYSEAVIGGKGADIDNAKKRMFRQLSS
                     ELMTLARLQFIISVVVYLICVILLPGEGFSGMTLKIYPSLAVGYFILFLMYAEIIFLY
                     YFDDLTGAIWTAIVFCLVTWIGSLIATHLPVIWYGAGVIAGAFAGWSVAYARLRWVEK
                     NMDRHVFCRGNLIPQGKGKRPSGMVYERKAEQEQKEEA"
     gene            complement(12685..14148)
                     /gene="pelF"
                     /locus_tag="PLN77_01255"
     CDS             complement(12685..14148)
                     /gene="pelF"
                     /locus_tag="PLN77_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HRM42940.1"
                     /translation="MRVCIVAEGCYPFVVGGVSSWVHSLIQLFPETEFVILAIAANRS
                     QRGKYAYELPKNVSAVYELYLDDVDWSGSKKLHRKRLKKEQYQALRSLILDQDIEWDV
                     LFDLFQKENVSLNALLMGEDFLNAVRDCYNLKYSQIVFSDFLWTMRSIYLPLFLTMQT
                     EIPRADLYHCVATGYAGVLGAMAKHFYGSRLLISEHGIYTREREEELIKAKWVEGIYK
                     NIWIDQFRKMSKLAYNEGTLITSLFEHARELQIELGCPIEKTMVTPNGIRVENLQNIP
                     GKTEEDEGKINIGAVLRVTPIKDVKTMIQAFGFAKEKDDRLKLWIMGPWEEDREYAEE
                     CFQLVSDMEIPDVVFTGRINIRDYLGRMDVTILTSISEGQPLTILESYAAHKPVIATD
                     VGNCYGLIYGESDDFGAAGIVTHIMNIEEIAQAMLDMAANEKMRLEMGETGYRRVMEK
                     YRVEYMQKTYWEIYRDFAEQMGLEWKEESVKLPDKEK"
     gene            complement(14145..16013)
                     /locus_tag="PLN77_01260"
     CDS             complement(14145..16013)
                     /locus_tag="PLN77_01260"
                     /inference="COORDINATES: protein motif:HMM:NF021477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2194 domain-containing protein"
                     /protein_id="HRM42941.1"
                     /translation="MVSRRNFVAITIIMAIVLFLFQGLNVAREKLNYYDTNAYAKDKE
                     TLADGSGAFGAVDASDQINIEGSRGTIVYIGAEKSDSIGTVVQEWCRYMKYESAVYSS
                     ITKYEKAMEKKDTRLPEMVIVNSENIDWTTVKDTVELQKCTEQGIHLVFANLPDVSVI
                     KKNQKFMELLGIKKITADQVTVKGMDLYANLMLGGENIYEAKKQEEKKMQDLELTFPW
                     FKLAGGTKAYMKGIPEDSTLKVQEHPVAIWRKSTGNAYVFAVNGDYMEDETGLGILTG
                     MLYETRDYLIYPVVNAQNLIAANFPVLTEENTAQMQEIYGNTATAVNRDIIWPSFASV
                     YEKNHLGLTCMLAPKLDYSSTTKPDGSMLNYYVKLINEQKGETGLSGTCESETDVIQK
                     LQEDQEFMQKKLNTFAFSSFYAGEQTDEEMEKALQQPVLEQIRTVVKAKDTEGDIVRY
                     QNDQVTSQKVISEEEEQFTYRSWMKVKSVESALGYTSVLLDLDAILYPKTEEDRWENI
                     GKEFAANLSTYWKLFSGFDGTTVSECDTRIRTFLNSRYEDDREDNAITLRTTGTDETA
                     YYVLRTHNEDVRKVTGGTAEKLEDSAWLIRAEQSEVRITLGASDQRYYYEKGAKNE"
     gene            complement(16016..18361)
                     /locus_tag="PLN77_01265"
     CDS             complement(16016..18361)
                     /locus_tag="PLN77_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42942.1"
                     /translation="MTMKWLTILQFLEIVAAYGIVTLFLPWLVLRKRFRRFSAAEQLT
                     GYFFAGNFYIIYLVYLLQFLHISNRVTLTAGTVGPFLLVWIWKRRSKIPGVIEWVLEN
                     VERLLSGERGKKTSLVQLRGTIYRRFFRGKRKQWLNVLLELAVMGAAIAAITYVYGPN
                     MIEAYGYKASDIPVHNYWINELDRNNIWVAGVYPYGFHIVIYYLHMVFGIKTYVLLRI
                     FGVVQTYFVYLAMVVALKMVCKGRFTPYLGVLFYVMDIFNRNTYARFEGALPQEFSMI
                     FILTSVCMAIRFFQEFAKEQKAEENEKKELDQNCRWYLIQFAIGFSLTLTIHFYSTMI
                     AGLFCIGVAVGFCFRFARWKYFRRIMATGILSVLLSVLPMAAGVAMGKGLQGSLYWGL
                     SVINGGKDDETEATDSLVENTELSTISESEGTENRDGGSTVSASQVEALKKAAEEKLA
                     EQAKEQQKPLKERLKEKFAAVRSSIRVNVMNVKPYIVEITLGSIVLLAALGSLMVLLR
                     EIDFAGVIFSTAFYLLCMCCLQCASLLHLPALMDPNRCSIFLCYSVCFSWTLGADAIV
                     HLLIRWWKKRWVSNVASILTLVIAVSAVISNGLLRKPIVVDALEDNGAIACVTNILKE
                     NRNFTWTICSADDELRMTEFYGYHYETITFLRELQDLEKNPEIIMPTQNVYFFVEKIP
                     IDYAGSTNHKEVTVEGAEAPLPSAGGIEPYFAENRWYTMCHIYYWTQKFKELYPDEME
                     VYYETDSFICYRVQQNVNSPYNFAIDYGYNNPTPKAGSEEE"
     gene            complement(18402..19358)
                     /locus_tag="PLN77_01270"
     CDS             complement(18402..19358)
                     /locus_tag="PLN77_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42943.1"
                     /translation="MSINGTLAFVILWIVNALIALVYLLVGIFLYVPACDLKREQGEE
                     IQYDNRRAFFIRFIVMVLCPVVGPAFFLCSYLIYKTVFRQAVDLEDVIFSKERVQTHL
                     KADEERERNMAPLEESLAVSDKRNMRMLMLNVIRGDMQKSLESITMALNSEDSETSHY
                     AASVLRDELNDFRSNVQKMYTQMQQETETETECEEMLIDYMNRILSQKIFTTMEQTKY
                     VNMLEEAAESLYQKNGARITADRYEGLCLKLLDLKKIPETEKWCMRLARQHGNALAAY
                     TCRLKLYFTMGEKEKFFEVLQELKESDIIIDNETLELIRIFS"
     gene            complement(19355..21535)
                     /locus_tag="PLN77_01275"
     CDS             complement(19355..21535)
                     /locus_tag="PLN77_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM42944.1"
                     /translation="MIVFLVGGESRLMDALITKMEKDGHKTYLLTGKRDGRGKYRRVF
                     ERYNFPYDSESVREIFTNVNPDLVIFLGAYDTNYDWSDARRESVRYTADLTNLLSAYA
                     FKQNGRFVYLSSEVVYGRSYSSDIHETEPASSKSFQAMAILQGENICKSYRDTREMDT
                     RVLRFDHMYDIPRKGKADNNPCFQMMLEMLKTNQIAANSRNVFSMIYQSDAVEFAYRV
                     MMAEHPKYPLYHISSGEVISQMKLAELIQQNAGDGTTIRDDTVGEGYRLVLDGRRYRK
                     EFDGKIFVPYEEGVKAVVEYMKKYSSSFLQREDTGAGWGNRVWRLLVRIFRMLAPYLE
                     NLICFIPFFMIHNRAVGSQYFAKLDAYLLYVLLFAIVYGQQQAIFSALLATAGYCFRQ
                     MYNQSGLEVLMDYSTYIWMAQLFILGMVVGYMRDQIHHIKKDDQEEIGYLHGQLGDMT
                     EINDSNVRMKQIFELQLVNQKDSLGKIYEITSSLDQYGAYEVLFYAAQTISKLMNTED
                     VAVYTVANQSYARLFSFTSPTARKLGNSIRYPEMEAMYTDLKERRVFINKTMDERYPL
                     MAQAIYAEDEMQIILMLWGLPWDRMNLAESNRLTVISYLIQNAVVRANRYLEALREHR
                     YLGNSSILEKEAFTQLVSAFFEAKRNGLTECSLVKITCGEEKYLEAAEVLGKKLRQTD
                     YVGMLDGGLYVLLSDTEEKNALGVITRFAEAGYESTIANGEVAA"
     gene            complement(21564..21833)
                     /locus_tag="PLN77_01280"
     CDS             complement(21564..21833)
                     /locus_tag="PLN77_01280"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRM42945.1"
                     /translation="MTRKGDFVLRQIADEYVLIPYGATAEKMNQVLTLSETAAFIYEQ
                     TGDAVSAKEIAKRLGKAYDMPAEEVQEDVNAVLETFRNYGILERV"
     gene            22051..23247
                     /locus_tag="PLN77_01285"
     CDS             22051..23247
                     /locus_tag="PLN77_01285"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM42946.1"
                     /translation="MQPLDAVYLQILKNLCTDLSEPVPLDGVDPSALYRLAEKHCSLP
                     FLLPYFEQQPQFSALKQQTKQMLLSYYQLEHFTRLTFSLLLAEKIPCFLLKGISLAAN
                     YPIPEYRKLGDLDLYIPEKDAFSRACRILNAHGYTEEPEESDHHVTYRFTFPETGRSF
                     TLELHYRIVGIYQFSRANELVDEIFSASHLKPSFVELYGQTYPVLPPTENVFYMLHHM
                     LKHYLYSGFGSRLLCDFTLYLERYASEVNFEKIHFWCRESKIFHLYELILESCRIYFG
                     LSASIDPQVHCSASDCAIFLEKILADGDFGSDTQRKIVSSGSYQHTGIHAWFHEGHIQ
                     MKLRFPKLHKCVLLWPVLWVITFFCFLSNTYRIRHTTLRQTLADFRADNRKTKLLKIF
                     DNNDLQ"
     gene            complement(23366..24340)
                     /locus_tag="PLN77_01290"
     CDS             complement(23366..24340)
                     /locus_tag="PLN77_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRM42947.1"
                     /translation="MTFKKSYVPGILGSVLTLTVIMAAPVKAETKEETILNKTDWSTM
                     AAANVEGYANIRSEASVDSEIVGVLMPGYAVTVTEKGDEWSKISSNGVEGYIKNEYLV
                     FGEEAKAHYRNMCGITGVVQADSLRVREAASTDSAQVGTLTQNGEVSVFGEEADWYQI
                     QYSGSSAYVHGDYVTLSEELKGAVSMEEYQASQACAASSAAAASTAGSASVISADSND
                     VAMLAALIECEAGGESYTGMVAVGAVVVNRVNSGSFPNSISGVIYQSGQFTPVATGTF
                     QSVLARGARSDCYAAAQAALAGESPVGGCLYFNSGYGSGIQIGYQHFY"
     gene            24903..26030
                     /locus_tag="PLN77_01295"
     CDS             24903..26030
                     /locus_tag="PLN77_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42948.1"
                     /translation="MLHSTQTTKKHTGNLILRFGMAGLLAAGLFFSAPYSAAAETVPS
                     AENTDAGEITIHFTHYDAQDLEFYLNTDQAALAFTDAALTFQDSTMDAPVTMSYLSDD
                     GSYVLYQNSEPVEEVNGTFSVSMVTEPGYTLDMDQISFYVNGQAVSNGGSVVITDSSA
                     AMTSLVYCTAIPETPATDPEPSLPEEPSAPEVPEEPAAPEPSVPEQPSAPAETPTVVQ
                     PSASEQPAQENILPETAAPASKTAPEQVPASEPIAAEPIASAVVTEDEEVDLSTIHPE
                     VTASAVTSSSQLPAATQIVPSVRTTDPDAQIITSETAALENAAQTENTQTAHTGIGSW
                     FHQRTNQILAVMIAVILLLILIESLLALKDSMNQKRSHRFY"
     gene            complement(26120..26845)
                     /locus_tag="PLN77_01300"
     CDS             complement(26120..26845)
                     /locus_tag="PLN77_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM42949.1"
                     /translation="MDTLYIVIPAYNESENIKRTIEEWCPVVEKHNGGGASRLVIIND
                     GSRDDTYEIASAECETHPLLTVLTKPNGGHGSTVLYGYRYALENGADYIFQTDSDGQT
                     DPEEFEKFWQIREGYDAIFGNRTSRGDGFSRAVVEKVLCAVLWTYFHVSVPDANAPFR
                     LMKTDYVAEYLPLMPENYNLPNALLTAFGAYFHRKIRFIPISFKPRQGGTNSINIRKI
                     VKIGRQALHDFCEIQKAAAKKRG"
     gene            complement(26944..27873)
                     /locus_tag="PLN77_01305"
     CDS             complement(26944..27873)
                     /locus_tag="PLN77_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM42950.1"
                     /translation="MSKKPVLVIMAAGMGSRYGGLKQIDPVDPYGNMIIDFSIYDAKQ
                     AGFEKVIFIIKKAIDEAFREGIGKRIEKYMDVEYVYQELDVLPKGYEVPEGRVKPWGT
                     GHAILCAAEAIDGAPFAVINSDDYYGRTAFEEIYRQLTTEQDGATYQYAMVAYDLYKT
                     LTDNGHVARGICTADDQNHLVGIHERTRIENHGGQAEYTEDDGATWTGLPQGTIVSMN
                     LWGFTPSILIELKRRFLPFLENVYKTNPLKGEYFLPFVVDELLQEKKAEVTVLRSYDN
                     WYGVTYKEDKENVMAALQKLKDEGRYPQKLWEE"
     gene            complement(27889..29148)
                     /locus_tag="PLN77_01310"
     CDS             complement(27889..29148)
                     /locus_tag="PLN77_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM42951.1"
                     /translation="MKANTISGQRQYAFVSQFNYIREAGTEGEEKAACRIEKELSEIA
                     EKWGQGELQIRREPFEIETWQVDEAVFTVTEPYEKTYTVRGCFAAANTAPEGVEAPFL
                     YVENGDPVSLSHAEGKIVLINGGANAENYEKLEKAGAVGFLILTGTPLDKDEDRLPDY
                     RTLRGVKNPKLPCAVIHYLDAMELVERGASRARLVLQQKKIRRTSKNIILRIPGTEQP
                     EEILTLTAHYDSVPQGPGAYDNMAGAAIIMELAHYFAENRPKRTLECIWFGAEELGLC
                     GSRAYVKAHEAELAQHRFNMNVDLAGQAIGGTVLGVAATKEACDVIMEHLKQADKGVS
                     LINNIWSSDSNTFAWKGIPAMTLNRDGFGMHTCHDTIDWISAWSLNRSAGVLGEIAEY
                     LADAEPFPFEREIPADFAERLKVYFGE"
     gene            complement(29187..>29576)
                     /locus_tag="PLN77_01315"
     CDS             complement(29187..>29576)
                     /locus_tag="PLN77_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HRM42952.1"
                     /translation="VTLHRAFDVCRDPKEALEQAVSLGMNTILTSGQQNSAVKGAELL
                     AELQRQAAGRIRIQAGGGICADAIRELYPKTGVTAYHMSGKIELASPMQYRKENVNMG
                     LPSLSEYTLYRTDVKAVRSARAVLEEL"
BASE COUNT         7200 a         7910 c         5957 g         8509 t
ORIGIN      
        1 ggacagcagc cccttcgctt tcagggacag caacctgtcc cgcaggtggt agttggacat
       61 gaccgtgtaa tctctggtct tttcgatacg gaatacagcc atcttcgcac ctccttcctc
      121 tgaaaatgaa aagtgttgat ttcgatgaaa aaagcctccg gtttgcctct cggatgtgat
      181 ccataggaaa acatccatgc ccgcctgctt acggtggtct gagaggaaaa aacagaggct
      241 ttcgcaccct aagtgataca gttttgagac agggacaggt gcgggtatga aaaaacgcca
      301 tgggacggtt gcctatggcg tgaaaacagg aaaagggcgc tgatatttgc atatcaacgc
      361 cctttgagcc tgtcagtatt tgattgtttc gacttcgcac cgtgtttgca ccatgaaaaa
      421 cattgatttt actgacttct tcaatgtttt cttatctggt gctaccattt ggcattccat
      481 tttttattcg cttgtaacct gtttttacaa attcggctcc aaattttgca ggtactttca
      541 attctgcaac tttcttcaat tttagaaatc cttctccatc tccaaaattt ctttctcgcc
      601 cagctcctca cacttctttc ctcgaatcgc atacactctc gtacagattc caagcaccgt
      661 cggccggtgt gtcgttacaa tgcaggttcg cggatacgta tctgtcataa tatttttcag
      721 cactttccgt tccgtcgcca catcgagcgc cgaagttgct tcatccagca gcaaaatcgg
      781 agattttcga agcagtgcac gcgcaatcga aagccgctgt gcctgtcctt ctgagaatcc
      841 gccgccgcgt tctttcacca tactttcaat accatccggc agtttctcca caaattccca
      901 tgcacatgcc agtttcaacg cctccacgat ctcttcatct gtggcttccg gtttcacatt
      961 tcgcatattt tctgcaattg ttcctgaaaa catcgtattc ccctgtggca cataagaaaa
     1021 caatttcctt gtggacggcg acagtgcaat ccgttgttct ctgtcttccc ctgcaccaat
     1081 ccatgcttct cccccctggg cacgcagcaa ggataagatc agccgcagca tcgttgtctt
     1141 tccctcgccg gacgggccga cgagtgcaat aatttcatgc ggccacgctt ccagtgatgc
     1201 tttcgaaaac acttctgttc ccgtatggta ggtatagctc aagtcctcca tagcaagccc
     1261 gatgccgctt gcccggtatt tttctgcaaa ctgctccact tcccgatccg cactgtagtc
     1321 ctcctgcggc atctccacaa tatccatcag acggccggct gagatgccaa gagaaatcgc
     1381 cgatggcaca aggctgacca gcgaattgac ggaacccgtc agtgtaccgg acagagacag
     1441 aaacatggtc atggttccgt aagaaattgc cccgctccag acacggtaaa ttccccatcc
     1501 gtagcagctg taagatacaa gatagctgat taacgtcatc agaagcgagg ttgcaatcga
     1561 cattttctgg aaatccaggc gcattttgat gtattcctgc tgcagatttt tcaggcgatc
     1621 gacataaaag cggatcatat cgaatgcctt gattgtctga atattggaaa atgcttcctg
     1681 attaaatccg taaagtttcg cactcatctg tgcgctccgc tggttattgt tcgtcatccg
     1741 gcgcagcagc gggcgggaca gaagcgcact gaacgggatg ccaagcaatg caaaaatggc
     1801 aaaggtggga tcataatgta ccacaatcgc caatgcactg ataaatttca cagtatagat
     1861 cacaagattc ggcagccagt tcaggatacc actggaaata tttgaagcat cagaactcca
     1921 ccgtgttacc agatctccgg tatgatacgc tgtcagcgat tcccagtccg tgaccagaat
     1981 cttggcaaaa atatcattct ggatttcgcg gtctaccttc aggttaatca tcgtagatgc
     2041 atacgcagat gcctgcgaaa tcagggtatt tccaaaagaa aagccgatca tcagtgcgaa
     2101 ggtctgcaaa agttttcctg tctggtgccc ggtgataata tcaaccagat ctttcgatat
     2161 cacactggac gcaagagaaa caccggttcc gaaaacacca agtgcggtat agaaaatcat
     2221 ggctttccag tatcgtctgg catactgtgc aatccacaga aattctttcc acatcttctg
     2281 aagcatttcc gcctggaggc atttttttat ttttgtccac aagtttctca ttttaaagcc
     2341 tcctgcgcaa gttcatcctc cgtgattctg tgatgcatca cttctgccgc tgtatcattt
     2401 ttcgtacagc gcaggaagta aaccggcttt tcatttgcaa tttcttccgc aaaagcaaga
     2461 ttctttttca tcagccctgc cgtccagggc ggcgagatca tccgctgcat cacacggagt
     2521 gttttctgct ctttcgtcag ggaaacgatt ttatcctccg gcgctttttc cagcagcaca
     2581 atccctccca gctctttctt ttcgactgtg aaaatttctg atgtgccgca ccacgggatt
     2641 ccgtatacta caaactgatc gccctctttt cctatgaggt tcagatctcc gttcagaaac
     2701 ggcgtgtcaa acagtttttt ccaaagtgcc gtatgagtcg attttcccat tcccgacgga
     2761 ccggaaaaca gccatgcttt ttccagataa agaagagatg cggaatgcag cacaaacatg
     2821 ccttttttct gtgccaggaa cagaaatacc ggccggatcg ccagaaagag ggaatcctgc
     2881 tcttcttctg tcataggcag acggtaataa atacaggcga agcttccatc cgctttcatc
     2941 cagatttcat agatattttt aagagccggg aaccagagga catagccttc ttcccacgcg
     3001 cagactgtca gatctttatt ccggatcaga atgtttccat tctggtggct ttccggtaca
     3061 tgctcgatca attctaacgt gagatccgcc gcatcctgcc tattttctgt ttttatcacc
     3121 gtttcttcca cttctttttc tcctgttttt ttcgctgcct ctccttctgt tttctctgtt
     3181 gcttcttcct ttcctctttt cttctcaaaa gaagcaaact gttttggaat gcagccctct
     3241 tttccataaa cagcaatgga aattcctgcg atcttcaatt ctccggcgca agttccatcc
     3301 ggagaaccaa gttcccgtcg taccgcacca aaggcaagaa gctccttcac aaaagcctga
     3361 atatcttttt tcagttcctc ctgctcttct tccggcactt cataacagcg aatcattttc
     3421 tgctgaagtt cttccattgt ttttggtgtt tccagcgctt cccagagaat ccgtgcggtc
     3481 tcattcagct ccattcctcg ctgctggtct gctattttct gcccataagg cagaagataa
     3541 tgtgtgttcc cgattttctg cagtaaatat ccttcctgcc ttctgattct cataatctga
     3601 tttcccttcc tgtgtttgtt ttcttatgtt tgctgtcttt cgttttgtat gtaaagttat
     3661 gctttttctg ctcatcatcc tttttatttt cagggtaagc tgttataata aaattcaggt
     3721 aactattcag agccactcgt caaaatgcca cgcatttcgt cggttcggcg tatccgccaa
     3781 atggagtttt ttatgtacac ttacgatatt gaacaacttt taaaaaacgg ttctgccgtc
     3841 aaactgaaac cgcagggcta cagtatgtat cctctttttc ttccgggccg ggacgaagct
     3901 gtcattgaac actgtgttcc ttccgaactt catcgcggcg atgtaattct gtatcgccgt
     3961 gtgcagggaa tccttgttct gcaccgcatc tgccgaatta ccagggatgg cttctatctg
     4021 gtcggggaca atcagaccga agtggaagga cctctttccc cggagcagat tcatggaaaa
     4081 ctgactggtg tcgtacgcaa tggaaaatcg ttttctgttt cgcatccaat ttatcggatg
     4141 ctttcttcca tctggctctt tttacgccct gtccgcccgt ttttctggtc ggtaacggct
     4201 tttttccgca ggttttttca gaagcactga ttttttcatt gtcctctccg aatttttttc
     4261 aaaatgcaca gagaaatttc tattgcatca ggcttttcgc attcccttca ccatggttac
     4321 aatgctctga agtctccagc ctcttccaaa gatcaccaga tccagcagca gcggaatcgc
     4381 aatacacagg agaaagcaga cgcagaacag aagcataccg cctgctccgt atatcagtgg
     4441 tacgattttc tgcatcagca gaacttcgcc cgctatgact gccagatact ctccgatatc
     4501 ctgcgcatac cgcagcgcct ctcccggcat ctcgaggaaa tcccggaaat aaatgatata
     4561 ccgatacaag atctgcagca gataagacag taaggttcca agcagaacgc ctgccatccc
     4621 atacggtttt acaagtgcca gtgacacgat cagattaaga agtgcggagg atgctccgac
     4681 atttttctct ttttcaaaca gtcccgtcac gcccatcaca aggctgagcg gcaatcccat
     4741 aattcgaacc agcaatacta caatacaaaa aacagtaatt ccccagccaa gttcgtagga
     4801 aggaccaagc cagatatcac ggacaaatgg tgtaatcaga gaaaacaggc caaccgatac
     4861 aaccgacgca atgcagaaaa agccaaaggt caattgccgc agaatctcat aatttctctg
     4921 acgatttttt tctgtaaaat aatctccgac cgtcggctgg atcgtattcg caaggatcgt
     4981 gagaatattg ctgatcgcct gcgtaatcat acagtaattt gcatatcgtc ctaccgtacc
     5041 gaccccgata aaaccggaaa taatcaggtt gtccgtgcaa tacagcattt tttctgaaat
     5101 tctggaaata aacaggcttt tcagatctct tacaattctt ttcgtcagag acgatgtgct
     5161 cttttctttt ctccgctggc gcaaaaacgg atatttctgc tccacatacc agctgatcca
     5221 gatattcagc acgacttccg ttacggttcc aataccaagc gccagcagat aattctgttc
     5281 ccatgtaacc agcagaataa tcgccagata gctgaccaca tttaaaatca gtccggtgat
     5341 gcttgcaatg tattctttct gatccgcaat cagcaacgac ttccggtacg ttaaaagata
     5401 agaaagaacg gttcgaacca gccacatcgc atacagaacg cggatatagg ataacgaata
     5461 actgttattt ttcataaaaa ggtggatgaa cggcagaacc gccattcccg ccgcaaatac
     5521 aagaacagca aagacgtgat atgcctttcg gtaaagattc atcagcgatg caatctctgc
     5581 ctcatccttt cttgcaagcg cgccatacaa atggtaaacg attgccgtgg atatgccaag
     5641 ctccgtcacg gaaaggatat tgatgacatt ggaaataaca ccattcattc caaccagctc
     5701 cgcgcccatg tacaggttca tggcacgctg gctgaaaaag ccgaggatca gaagcgcaaa
     5761 ctgtgaaagc atactgaaaa aactgtttcg gaataaattt ttaatgcgca taagttcctc
     5821 cttcttctcg tttttataac atgctacgcg ggtaaacagt tccgtaattt tacatcactc
     5881 ttattttcca ctgttttcat ggattttgta acattaaaat ggtatcatat atctaatacc
     5941 tgctatcgtt gctgccgttt ccagatacct gtgaatggca gtatcccttt tggaaaggct
     6001 ttcctttatg aaaacaaata gaaaaaaatc attttcttat cttctgtttt ttcttctgct
     6061 tctggtattc cttgcactgt tcgcatttct tggtatccgt tctttttatc gtgcgaatta
     6121 catgccggaa ccatgcacag aatccgcaga cgagttatcc aatccataca ttggatttta
     6181 tcacatgtat ggctatgttc ttggaagcga ttcgctctcg aatcttcctg ccgcctctga
     6241 ttccgacgcc aaaaacaggc agggaattgt catgctggaa gtgaatctgc agaatttttc
     6301 aaacgaagat ctctccgatt cagctctttc ccagcttgat accattctct ctgcctggca
     6361 gagacatggc agtcaggtga tcttacgttt tctctacgac tgggatggaa aagcaatgga
     6421 aacagagccg caaagtctgg agcaaattct gcgtcacatg gaccagacag ctgaagttgt
     6481 aaatcgctac acggactgtg tttttctgat gcagggcatt tttgtcggaa actgcgggga
     6541 aatgaacaat tctcactaca tgtccgatga ggactgtact accctgatgc accatctggc
     6601 agaggtaacg gatccttcgg tttttctgtc tgtccgcaca ccggtacaga ggcggaaaat
     6661 tctggattcc tccgaacggc cgacaaagga aacagctttt gatggttcgc tttcctcccg
     6721 gcttggactg tttaacgatg gtatgctcgg aacggccaac gataccggca cttatggtga
     6781 taccgctgcc tccgcagaca cttaccgttc cgcctgggtt cgtgaggatg agctttcctt
     6841 ccagaatgaa ctctgcaatt tcgtcccaaa cggcggcgag gtcactctgg ataacccatt
     6901 gaatgatctc gcacatgcca ttcaggattt atcgcgcatg catgtctcct atctgaattc
     6961 ggaacacgat cccgcggttc tggataaatg gaaagcagct gcttacaaag acaaagcttc
     7021 cgtttttaac ggattgtccg gctatgatta tatcgagcgt catctgggat accgctatgt
     7081 catccaggac actgctttgg attcgtctga ttttcagata cggctggaaa atgttggttt
     7141 ttctgtctgc tatcgaaaac tggatttaca gctcaccctg gtttcttccg atggtgaggt
     7201 tttctcattt cccatctctt cggattccag attctggata ccggggacta ctgcagagct
     7261 ctctatttct ctcccgcttg cgagactcac caaaggcagt tatacggtat atttccagat
     7321 gacggatcct gttctgcaaa gggaaatttt gcccgcaaac acattttctc atgatacgca
     7381 tggattttcg attggtacgc tcgaaatcag caaattatat taaaacaatc gggtcaattt
     7441 tgaaaattcc caaaaagggg cgtccctgtt tttctccctg attggcaaat gaaatagttc
     7501 ttccatcccg cctgcggtac agcgccagat acagtttctg ttcttctggt tctgctaatt
     7561 ccgcaggcag ttttattttc tggcaaagag tggttttttc tccgctcttc cagagtctcg
     7621 catctgttgc aatctgtatt tttttctgaa gcgttccgtt tctttccagc tccagataac
     7681 agtctgcctc ttcggtcagt tctgcaaatc ctttgttttc cagtgtaatc tgaagttctt
     7741 ccctttccga aacaaactcc acaccttcca caacaaaccg atagccgaga cgttttccaa
     7801 ttgcctccag gccgctttcg tcctcgaccg tttctgcttt ccattgttcc agccgtcgta
     7861 aatcatacgt actgttcaga taggaaatat gcattctttc gagatccgcc attgcttttg
     7921 gggccgagac gaaaggagcc ggttctgccc cacttcccca tatggcctct ccgccgcaaa
     7981 attttccgca catttcgttt ttctgccatt ccagttcttc ttcccggctg cagattttct
     8041 gtcccggtgt ttccggtgac agtgtgccat acgttccaag atccgtttcc gatccgaata
     8101 tcccatcgtt aaacagagtc agttttttct tttcctgctc cgtccatccc aaagtcagtt
     8161 cccgccactg cgccggtttc cgcactgcaa ccgggcaggc gccgtccact gcttttatca
     8221 tggtatcggt cagggttcga aaggcatccg gggtcagata acgggaaccg tgcatttcgc
     8281 cccagttccc gatcagaatt ccctgtactg caaaaatatc gtccgcatat tcccgaatga
     8341 cttctccgat ctgctgcata tgttcttcca ccagacggat actgcccggc tctttctcca
     8401 gtccttttcc ctcaagatca taggaaaacc gcaggatcat tccttttctt ttctcccgaa
     8461 aaaatgccag aatccttcga atccattcca gagacggttc tgaaatcggc ttgtcccgga
     8521 atgctccgat atcaattctg agaaggacaa gttcttcctc ttcacaggcg atgtagagcg
     8581 gttccccggt ttgcgccgcg ttgaaagtat ataaatgata ccagccacac ccgggattct
     8641 gtatcgcttt cctgctttcc tgaagtatcg ctttctggaa aagctctttt ttatcctgtt
     8701 ttccccgcag ttctggaaat tctttctttt ctgccctgcc tttagcttct cttcccttct
     8761 gccagctcag gatacttttt atcttctctc tccacatcaa tccgtcctgc tcctgcatcc
     8821 attttcaata ccttacaccg gaaaatcaca gccaggacac tgtaaaacat ccgcatcata
     8881 taccagattg gcttcaaacc agaatgcatg cttttccccg aagtccgagc atgcataaca
     8941 gctggaatct ccacaacttt aaaatcaaga agcagcatct gagtaatcat attagtatcc
     9001 ggatatttat catcgaaatg attatatttg gaatagtata aaacagcttt ccggcttaaa
     9061 ccctgcagtc cggttgtcgg atccgcaatt ttccgtccag ttgccaaata cagcattttt
     9121 cgaaacagga caaatgccat tttttttgca aaggaaactt caaactctgt actccccttc
     9181 atgaaacggg atccaagcac aatgtccggc gttcttccat catcatcttt ttcctttaat
     9241 ctctggtaaa ttttcgggat attgcacaca tcgtgctgcc catccgcatc catctgaata
     9301 acataagaat agccccttcg gattgcatat ttatatccaa gctgcagccc actgccatat
     9361 cccagattaa aaacatgagt aaccaccgca cagccatatt ccttcgccag ccagttggta
     9421 ttgtcagaag aagcatcgtt catgaccagc acatcaacaa tatctgctat ctccggctgc
     9481 gccagctgtt tcatgacctt ttctatattt ttttcctcgt tgtatgccgg aataatcagc
     9541 agtacctctt tttgtctcat atgtatgtcc gcctcatttc attagattca gcagtttcca
     9601 gatttcctct gtctgaccgt tcattttctg ttttgccgct gctgcaagcc gttcccgttt
     9661 cttcggatca tccagcagtt cttctacttt ttcggcaatg ccttccggtg tcagcgtaca
     9721 gagaagacca tcttccccgt ccacaatctg ctctctgttt cccgcacagt ccgatgcaat
     9781 cacagcacag ccaagtgtct gcgcttcctg gatggcgatg ctttttcctt caaaccgtgt
     9841 tgcatgaacg tagagatcac actgtgcata ataaggaaac agattctctt ttgcacccgg
     9901 cagctggaaa tcttcctgca ggccaagctc tgcaatctgc ttttcgagcg catttctctc
     9961 ggatccctct cccagtacat accatcgcgc ctcatagcca tcttcgcgga tctttttcat
    10021 ggccggaatt gcgatgtcat atgctttctg ataggccagt cttccgacag ttaagattcg
    10081 aattcctgta taggaatctg aaaaaccgcc cggcagctgc gcctcacgcg caatccggtc
    10141 tgtatcgaga atattgtgaa aaacctgcac tttcttctca tattccggat aaaattccag
    10201 aaaatgacgg cgcgtctcct gcgaaacggt aaagatctga tccatctttt cgtagcagtc
    10261 ccgatccatt tcccttgtat aaccggaatt gccgtatgca atatggacaa aggcagcttt
    10321 tttcttagca ttcacatgat cggcaacgta ataagcagaa ccgccttcca ggtaagcaac
    10381 cgccagatcg aaggtttccg aaaatcggtc tgctccatcc gaaattacac gccagagcag
    10441 cttgtccggc tgaatgtttc cagttttttt cattcggaaa aaattttgta tcacatattg
    10501 cattttcttt atggtgcgac catggcggaa aaacgccaga aaagatgtct gtatcagttt
    10561 tctccgcccc gcaccggaca gcacagaagc ggtacagatt ttcttatttt tcacatgtac
    10621 cctctgcggt acctgggaaa tcatttcacc ctgccccaaa agtacgtaaa gtgaaatttc
    10681 atattcctcc gcatccaggc ggcgcaaaag ttccagcagc gctgtttccg ctcccgcccg
    10741 gccaagggtg ttgatcacaa acaaaatttt cttcattcgt tcttccttct tctattccct
    10801 tataactctc ttgcattttt gcccgtcagt ttttccagtt ctctcagaat tctttcattc
    10861 tcctggttca aaagagaaac cagaatcgcc agttcctggt ttttcattaa cagggaagag
    10921 atctgaattg tcatctggta aacgccccac agaaccagaa gcgctgtgat cagaagaaca
    10981 acgaggctcc caagcccgat ccggctgctc catccggaaa atctcggaac cgctccgctt
    11041 aagattgctg caattccaag cagttcccag gtaactgcca ggtttacggt cagcttcttt
    11101 ttcgcatgaa gcacgaaggt aacgatcata attccaattc ccatcaaaat aacgcaagct
    11161 tttacaattg ctgctgtgct catttacgct tcctccttct gctcttgctc tgcttttctt
    11221 tcataaacca taccggatgg ccgtttcccc tttccctgag gaatcagatt tccacggcaa
    11281 aagacatgtc tgtccatgtt tttttctacc catcgcagtc tcgcatatgc cacactccat
    11341 cctgcaaatg ctcctgctat gaccccggcg ccataccaga tcaccggtaa atgtgttgca
    11401 atcagactgc caatccatgt caccaggcaa aacacgatcg ctgtccagat tgcccctgtc
    11461 agatcgtcaa aataatacag gaagataatt tccgcataca tcagaaacag aataaaatat
    11521 ccaaccgcaa gtgacggata aattttcagt gtcataccgg aaaatccctc ccctggtagc
    11581 agaataacac agatcagata aaccacaaca gaaatgataa actgtaaccg cgccagtgtc
    11641 atcagttcac tggaaagctg acggaacatc cgtttcttcg cattgtcaat atcggcgcct
    11701 tttccgccga tgactgcctc cgagtacagt ttatattttt cgtgaaagtt catctccaca
    11761 cgcgcaataa agatgatcgt tgcggaaatg ttggtaaaca tggcgagaca ggatgccata
    11821 tcgtatggct ggttacatac gaaacttttt gcaatctcca tccgcatatc cgttgtccag
    11881 aacacaaaat tgtgaatgta taacccaagg atatacaaaa agtttgtaaa aatcaatttc
    11941 caatatttcc taaaatactg cagtaccggc cggtaatgat tgctatttcg cacaaaatac
    12001 cggcggatca gcgcattttc cagcgttgcc gtcacaaaaa atccgatcgt cagagaaaac
    12061 agcatactcc atgttgtctc ccacttcaga ataaagtgta aaatcagggc aaggacaaat
    12121 gccaccagca tgccgaagaa aaaatactgt gaaatccgtt gataatcttt gcaaatagaa
    12181 agataaatca tcgagtaaaa aacaagcacc agactgatat aaccgcaaaa gccacagaat
    12241 acatatgcgg ttggtacctt tcccacaaaa tgttcccaga gacagaacgg aatccccaac
    12301 agacagctga gaccaatgtt cagaagcata ccaatatgat agcagggtaa aatatcctga
    12361 taccgctctt cgtagatcac atcagacata tagcgggaca gcaccgcatt aaacggggac
    12421 gccgtcagaa gagaaaagat aaaggtataa agaatcgtgc aggaaaacag ctctcttgtc
    12481 acatatccca cactggcaaa atccagcaga tattccatta aaatcagatt gataatgatg
    12541 acaaacatgg gcgcaaccgt cactacggtg ctgtagaaaa atccaaccag atttgtcgta
    12601 agtgtatttt tttcaaaaat ccgattcagc cgaactccga ttcctgccat atcctcttcc
    12661 tccttttttg tgcctgctcc ggttttactt ttccttatcc ggcagtttta ccgattcttc
    12721 cttccactcc agccccatct gttctgcgaa atcccggtaa atctcccagt atgttttctg
    12781 catatattct actctatatt tctccatcac acggcgatag ccggtctctc ccatttccag
    12841 gcgcattttt tcatttgccg ccatatccag catcgcctgc gcgatttctt caatattcat
    12901 gatatgcgtg acaattccgg ccgcaccgaa atcatcactt tccccgtaaa tcagtccata
    12961 gcagtttccg acatccgttg caatcaccgg cttatgcgcc gcatagcttt ccagaatggt
    13021 cagcggctgt ccctcactta tgctggtcag aatggtcaca tccattcttc caagataatc
    13081 gcggatattg attcttccgg taaaaacaac atccgggatt tccatatccg agacaagctg
    13141 gaaacactcc tcggcatatt cccggtcttc ttcccatggt cccataatcc agagtttcag
    13201 acggtcatct ttttctttcg caaaaccaaa tgcctgaatc atcgttttga catctttaat
    13261 cggggtcaca cgcagtaccg caccgatgtt gatttttcct tcatcttctt ccgttttccc
    13321 cggaatattc tgcagatttt ccacacggat tccgtttggc gtgaccatgg ttttttcgat
    13381 cgggcagcca agttcgatct gcagttcccg tgcatgttca aacagtgatg taatgagcgt
    13441 cccttcgttg tacgccagct ttgacatttt tcgaaactga tcgatccaga tatttttata
    13501 gattccttct acccatttgg ctttgatcag ttcttcttcc cgctctctgg tgtaaattcc
    13561 gtgctcagaa atcaggagac ggctgccgta gaagtgtttt gccatggcac caagaacccc
    13621 cgcataaccg gttgcaacac agtggtacaa atcggcacgc ggtatttccg tctgcatggt
    13681 gagaaacagc ggcagataaa tcgaacgcat cgtccacagg aaatcagaaa agacaatctg
    13741 actgtatttc agattatagc agtctctgac cgcattcaga aaatcctccc ccatcagcag
    13801 cgcattcagg gaaacatttt ctttctggaa aaggtcaaac aaaacatccc actcgatgtc
    13861 ctgatccaga atcagactgc gcagtgcctg atactgttct tttttcagcc gttttctgtg
    13921 cagtttcttg ctgccgctcc agtccacatc gtcaagataa agttcataga ccgcagacac
    13981 atttttcggc aattcatagg catattttcc ccgctgggaa cgatttgccg caattgcaag
    14041 aatcacaaat tccgtttccg gaaacagctg gatcaggctg tgtacccagc ttgaaacacc
    14101 tcctacgacg aaagggtagc atccttccgc aacaatgcag actctcattc gtttttcgcc
    14161 cctttctcat agtaataccg ttgatccgat gctccaagcg taatccttac ctcactttgc
    14221 tctgcgcgaa tcagccacgc ggaatcttca agtttttctg ctgttccgcc tgtaactttt
    14281 ctgacatctt cattgtgcgt gcgaagaaca taatacgctg tttcatcggt tcctgttgtc
    14341 cttaatgtga tggcattgtc ttctctgtca tcttcataac gggaattcag aaatgtccgg
    14401 attctggtgt cacattccga tacggtcgtg ccgtcaaaac cggagaatag cttccagtac
    14461 gtagaaagat tcgccgcaaa ttccttgccg atattttccc agcgatcctc ttctgttttc
    14521 ggataaagaa tagcatccaa atccagcaga acactggtat atccaagtgc agattccaca
    14581 ctctttactt tcatccagga acggtatgta aattgctcct cttcttccga tatcactttc
    14641 tggcttgtaa cctgatcgtt ctgatatctt acaatatccc cctcggtatc cttggctttt
    14701 acaaccgtac gaatctgttc aagaacaggc tgctgcaatg ctttttccat ttcttcatcc
    14761 gtctgttctc cagcgtaaaa agaagaaaaa gcaaacgtat tcaatttttt ttgcatgaac
    14821 tcctgatctt cctgcagttt ctgtatcaca tccgtttctg actcacaagt accggaaagc
    14881 cctgtttccc ctttttgctc attaatcagt ttcacataat agttcagcat actgccatct
    14941 ggttttgttg tactgctgta atcaagcttc ggcgccagca tacaggtcag tccgagatga
    15001 ttcttttcat aaacagaagc aaaagagggc cagatgatat cacggttaac agctgttgct
    15061 gtgttgccgt aaatctcctg catctgtgct gtattttcct ctgtcagaac cggaaaatta
    15121 gcagcaatca gattctgtgc atttacgacc gggtaaatca gataatctct tgtctcgtaa
    15181 agcattccgg tcaggattcc aagtcccgtt tcatcctcca tataatctcc gtttactgca
    15241 aaaacatagg catttcccgt actttttcgc cagattgcaa cgggatgttc ctgcactttt
    15301 aaggtactgt cttccggaat acctttcata taagctttcg taccacctgc cagtttaaac
    15361 cacggaaaag tcagttcaag atcctgcatc tttttctctt cctgtttctt cgcctcataa
    15421 atgttttctc cgccaagcat cagattcgca tacaaatcca ttcccttcac cgttacctga
    15481 tctgctgtaa tttttttgat gccaagcaat tccatgaatt tctgattctt ttttatcaca
    15541 gaaacatcag gcagatttgc aaatacaaga tgaatcccct gttctgtaca tttttgcagt
    15601 tccacggtat cctttacggt tgtccagtct atattttccg aattcacaat caccatttcc
    15661 ggcagcctgg tatctttttt ctccattgct ttctcatatt ttgttatcga tgaatagact
    15721 gctgactcat atttcatata ccggcaccat tcctgaacaa cggtaccgat agagtcgctc
    15781 ttttctgctc cgatataaac aatggttccc ctgctgccct ctatgttaat ctgatcggaa
    15841 gcatccactg ctccaaatgc tccgcttcca tccgctaacg tttctttatc cttcgcatat
    15901 gcatttgtat cgtaatagtt cagcttttct cttgccacat tcaatccctg gaataagaaa
    15961 agaacgatcg ccataatgat tgtaattgct acaaaatttc tacgcgaaac catctctatt
    16021 cttcctcgct tcctgccttt ggcgttggat tgttatatcc gtaatcaatt gcaaaattgt
    16081 acggactgtt tacgttctgc tgtacacgat aacaaataaa actgtctgtt tcataataga
    16141 cttccatctc gtctggataa agttctttaa atttctgtgt ccagtaataa atatggcaca
    16201 tcgtatacca ccggttttct gcaaaatacg gttcaattcc gcctgctgac ggaaggggtg
    16261 cttctgctcc ctccacggtt acttctttgt ggttggtact tcccgcataa tcaattggaa
    16321 ttttttccac aaaaaaatat acattctgtg tcggcataat gatctccgga tttttttcca
    16381 gatcctgcag ttcacgcaga aaagtaatgg tctcataatg gtagccgtaa aactccgtca
    16441 ttcgaagttc atcatcggca gagcagatgg tccaggtgaa attcctgttt tcttttaaaa
    16501 tattggtaac gcaggcaatc gctccattat cttccagagc gtctacaaca atcggctttc
    16561 tcagaagacc atttgatatc acagcagaaa ccgctatgac taaagtaagg atagatgcaa
    16621 cattgctgac ccatcttttc ttccaccatc gtatcaggag atgtacgatg gcatctgctc
    16681 ccagcgtcca tgaaaaacaa accgaatagc aaaggaaaat gctgcagcgg tttggatcca
    16741 tcaatgccgg caaatgcagc agggatgcac attgcagaca gcacatacag agcagataaa
    16801 aggcagtgga aaaaatcact cccgcaaaat caatctcccg taaaagcacc atcaaacttc
    16861 caagcgcagc caaaagcaca atacttccca gggtaatttc cacaatatat ggttttacat
    16921 tcatcacatt tacgcgaatg ctgcttcgaa ctgctgcaaa tttttctttt aaacgttcct
    16981 ttaatggctt ctgctgctcc ttcgcttgtt ctgccagttt ctcttcagcc gcttttttca
    17041 gcgcctcgac ctgtgatgca ctgactgttg agccgccatc cctgttttct gtcccctcac
    17101 tttccgaaat ggtgctcagc tctgtatttt ccaccagaga atctgtcgct tccgtttcat
    17161 catcctttcc tccattgata acacttaatc cccagtacag ggatccctgc agtccttttc
    17221 ccatcgcaac acccgccgcc atgggcagta cagaaagcag cacactcagg attcccgttg
    17281 ccataattct gcggaaatac ttccaccggg caaaccggaa acaaaagccg actgcaacgc
    17341 caatgcaaaa cagcccggcg atcatcgtgc tgtaaaagtg gatcgtcagc gtaagcgaaa
    17401 atccaatggc aaactggatc agataccaac ggcagttttg atccagttct tttttttcat
    17461 tctcttctgc cttctgctct tttgcaaatt cctgaaaaaa acggatcgcc atgcagacag
    17521 acgtcagaat aaagatcata ctgaattcct gcggcagcgc accttcaaat cttgcatacg
    17581 tattccggtt aaaaatatcc atcacataga acagcacgcc aagatatgga gtaaatcttc
    17641 ccttgcacac cattttcagc gccacaacca ttgccagata tacaaaatat gtctgcacta
    17701 cgccaaagat ccgcagcaag acatacgttt tgatcccaaa caccatatgc agataataaa
    17761 tcacgatgtg aaatccatac gggtacacac ctgcaaccca gatgttgttc cgatccagct
    17821 cattgatcca gtagttgtgt accgggatat cactggcttt ataaccgtaa gcttcgatca
    17881 tattcggccc atagacatac gtaattgccg caatcgcggc ccccatcaca gcaagctcca
    17941 gcagcacatt cagccattgc tttctttttc cacggaaaaa ccgcctgtaa atggtccctc
    18001 gcagctgcac aagcgaagtt ttctttccac gttctccact cagcagacgc tctacattct
    18061 ccagaaccca ctcgatcaca cctggaatct tacttcttct tttccagatc cacaccagaa
    18121 gaaacggccc cacggtgcct gccgtcagtg tcacacgatt gctgatatga agaaactgca
    18181 gcagatatac cagatagatg atataaaaat ttcccgcaaa aaaatatccg gtcagctgtt
    18241 ccgcagcaga aaaacggcgg aaccgcttcc ggagtaccag ccatgggagg aacaaagtta
    18301 caatgccata tgccgcgaca atttccagaa actgtaaaat tgtaagccat ttcattgtca
    18361 taatctctgt tcctcctttc tgcttttctt tttcttcctg cttaactgaa aatccggatc
    18421 agctccagcg tctcattatc aataataata tcagactctt tcagttcctg caaaacttcg
    18481 aagaatttct ctttttcccc cattgtgaaa tacagtttta accggcaggt atacgctgcc
    18541 agcgcatttc cgtgctgccg tgccagacgc atacaccact tttcggtttc cggaattttt
    18601 tttaaatcca gaagtttcag gcacagtccc tcgtaccggt ctgccgtaat tcgtgcaccg
    18661 tttttctggt acagcgattc ggccgcttct tccagcatat tgacatactt ggtctgctcc
    18721 attgttgtaa aaattttctg acttaaaatt cgattcatat aatcgatcag catttcttca
    18781 cattctgttt ccgtctctgt ttcctgctgc atctgcgtgt acattttctg cacattggac
    18841 cggaaatcgt tcagctcatc ccgcaatacc gaggccgcat aatgggatgt ctcagaatct
    18901 tcgctgttca gtgccatggt aatggattcc agggattttt gcatatctcc gcggatcaca
    18961 ttcagcatca gcatacgcat attccgcttg tcactgacgg caagcgattc ttccagcggc
    19021 gccatgttgc gctcccgctc ctcgtccgct ttcagatgtg tctgcacacg ttccttgctg
    19081 aaaataacat cttccagatc gaccgcctgg cggaataccg tcttgtaaat cagataactg
    19141 cacagaaaaa atgccggacc gacgaccggg cagagaacca tgacgataaa acgaataaaa
    19201 aatgctctcc gattgtcgta ctgaatctcc tccccctgct ctcttttcag atcacacgcc
    19261 ggaacataaa ggaaaattcc aaccagcaga tacacaagcg caatcagagc attcacgatc
    19321 cacaggatca caaacgccag tgttccgttt atgctcatgc ggccacctcc ccatttgcaa
    19381 ttgtactctc atatccagct tctgcaaaac gagtaataac acccagcgca ttcttttcct
    19441 ctgtatccga aagcaataca tacaggccgc catccagcat tccgacgtaa tcggtctgtc
    19501 gcagtttctt tccgagcacc tctgctgctt ccagatattt ttcttctccg caggtaatct
    19561 ttaccaggga acattctgtc agtccattcc gctttgcctc aaaaaacgca cttaccagct
    19621 gtgtaaatgc ctctttttcc aaaatgctgc tgttgcccag ataacggtgc tcccggagtg
    19681 cttccagata acggtttgca cggacaaccg cattctgaat cagatagctg atgaccgtca
    19741 gacgattgga ctcggccagg ttcatgcggt cccacggaag tccccacagc atcagaatga
    19801 tctgcatttc atcctccgca taaatggcct gcgccatcag cggatagcgc tcatccatcg
    19861 tcttgttgat gaatacgcgg cgttctttca gatccgtata catcgcttcc atctccgggt
    19921 agcggatgga atttcccagc tttctggccg tcggcgaagt aaaggaaaag agacgcgcat
    19981 acgactggtt ggcaacggtg taaaccgcca catcctccgt attcatcagc ttggaaatcg
    20041 tctgtgctgc atagaaaagc acctcatagg caccgtattg atccagtgac gatgtaattt
    20101 cataaatttt tccaagactg tccttctggt tgacaagctg cagttcaaaa atctgtttca
    20161 tccgcacatt gctgtcgttg atctcagtca tatcgccaag ctgtccgtgc agatagccga
    20221 tctcctcctg atcgtccttt ttgatatgat gaatctggtc acgcatgtaa ccgactacca
    20281 tgcccaggat aaaaagctgc gccatccaga tatacgtact gtaatccatc agcacttcca
    20341 gaccgctctg gttatacatc tggcggaaac agtagccagc cgtcgccaga agcgctgaga
    20401 agatcgcctg ctgctgtcca tacacaatcg caaacaaaag cacatacaaa agatacgcat
    20461 ccagctttgc aaaatactgg ctgccgaccg cacggttatg aatcataaag aacgggataa
    20521 aacagatcag gttttccaga tacggagcca gcatgcggaa aatccgcacc agcagtctcc
    20581 agacccggtt gccccatccg gcgcccgtat cttctctctg taaaaaggaa ctgctgtatt
    20641 tcttcatata ctctacaaca gcttttacgc cttcttcgta tgggacaaaa atttttccgt
    20701 caaattcttt cctgtaccgt ctgccatcca gcaccaggcg atagccctct ccgacggtat
    20761 catcccgaat cgtcgttccg tctcccgcat tctgctgaat cagctctgcc agtttcatct
    20821 gactgatgac ctcgccggaa gaaatgtgat acagcggata tttcggatgc tctgccatca
    20881 tcacgcggta cgcaaactcc accgcatcgc tctgataaat catggaaaaa acatttcggc
    20941 tgtttgctgc aatctgattg gttttcagca tttccagcat catctggaaa catgggttat
    21001 tgtcggcctt tcctttccgt ggaatatcat acatatgatc gaagcgcaga acccgcgtgt
    21061 ccatctctct ggtgtcacga tagcttttgc agatattttc tccctgcaaa atcgccattg
    21121 cctgaaagct tttggacgat gccggttccg tttcgtggat atcgctgctg taagaccggc
    21181 cataaacaac ttcggaagac aggtacacaa aacgaccatt ttgtttaaat gcatacgccg
    21241 aaagaaggtt cgtcagatct gcggtgtaac ggaccgactc tcttctggca tcactccagt
    21301 catagttggt atcgtaagct ccgagaaaaa taacaagatc cgggtttaca ttggtaaaaa
    21361 tttcccgcac gctctcgctg tcgtagggga aattataccg ttcgaagacg cggcggtact
    21421 ttcctcttcc atcccgcttt ccggtcagca gatatgtctt atgtccgtct ttttccattt
    21481 tggtgatcag agcatccatc agtctgctct cgccgccgac taaaaatact atcatacgct
    21541 acctcgccgt tttcgtagat ttttcaaaca cgctccagga taccatagtt tcggaatgtt
    21601 tccagcactg cgtttacgtc ctcctgcact tcctccgccg gcatatcgta agcttttccg
    21661 agccgttttg caatctcttt tgcagatacg gcatcgccgg tctgctcgta aataaaagct
    21721 gctgtttcac tcagtgtgag tacctgattc atcttttccg ccgtcgcccc atatggaatc
    21781 agaacatatt cgtctgcaat ctgtctgagt acaaaatcac cttttctggt catttttatc
    21841 tccattcttc tgcgtcttgc ttttctttct tacctcagag aaaaacaagg atactaatag
    21901 ttctattata gccctaccca attgcattgt aaactttatt ttgcttttta ttgatatctg
    21961 taataaagtt ttatttctct gcactttctt tttccatttt gtgatatact aaaacatatt
    22021 tgaaaaatct tttccccaaa ggagtatctt atgcagccgc ttgatgccgt ttatttacag
    22081 attttaaaaa atttatgcac ggatttgtcc gaaccggtcc cgcttgatgg tgtggatccg
    22141 tccgccttat accgtctggc cgaaaaacac tgttcgcttc cgtttcttct gccatatttt
    22201 gaacagcagc cgcagttttc tgctctcaaa cagcagacga agcagatgct gctctcctat
    22261 tatcagctgg aacattttac acgccttact ttttcgctcc tccttgcgga gaagattccc
    22321 tgctttctct taaagggcat cagtctcgcc gccaattatc ccattcccga atatcggaag
    22381 ctgggagatc tggatcttta tattcccgaa aaagatgctt tttcccgtgc ctgccggatt
    22441 cttaacgctc atggctacac ggaggagccg gaggagagcg atcaccatgt cacctaccgc
    22501 ttcacttttc ccgagactgg ccgctccttt acgctggaac tccattaccg catcgtcggt
    22561 atttatcagt tttcccgggc caatgaactg gtggacgaaa ttttttccgc cagtcatctg
    22621 aaaccatctt tcgtggaact ctacggtcag acctatccgg tactgccgcc aacggaaaat
    22681 gtgttttaca tgctgcatca catgctcaag cattacctct acagcggttt tggcagccgt
    22741 ctgctctgcg atttcactct gtatctggaa cgttatgcct ccgaggttaa ttttgaaaaa
    22801 atccacttct ggtgccggga atcgaaaatt tttcatctgt atgagctgat tctggaatcc
    22861 tgccgtattt atttcggtct ttctgcctcc attgatccgc aggttcactg ctctgcctct
    22921 gactgtgcca tttttctgga aaaaattctc gcagacggcg atttcggcag tgatacacag
    22981 agaaaaattg tcagcagcgg ttcctaccag cacaccggta tccatgcctg gttccacgaa
    23041 ggccatattc agatgaagct gcggttccca aagcttcata aatgcgtact tctctggccg
    23101 gttctctggg tcatcacatt tttctgtttc ctgtccaaca cctaccgtat ccgccacacg
    23161 acactgcggc agacgctggc agacttccgg gccgataacc ggaagaccaa gctgttaaaa
    23221 atcttcgaca acaacgattt gcaataaaac gaaaaacgag taagcgaacg tctcccctgt
    23281 tcacttactc gtttttctgt tctgtcaatc tatgggaatg cgtcttccct ctgcctatcc
    23341 ccttgcaaag gttccacgcc tgtttttaat aaaaatgctg atatccgatc tgaattccgc
    23401 tgccgtagcc gctgttgaaa tacaggcatc cgccaaccgg gctctcacct gcaagtgcag
    23461 cctgtgcagc tgcgtaacag tcacttctcg ctccgcgtgc gagcacactc tggaaagttc
    23521 cggtcgcaac cggtgtaaac tgtccactct gataaataac gccggaaatg ctgttcggga
    23581 aggaaccgct gttgacacgg tttactacaa ccgctccgac tgccaccatt cctgtgtagc
    23641 tctcgccgcc tgcctcgcac tcgatcagtg ctgcaagcat tgccacatca ttgctgtctg
    23701 cggaaattac cgatgcactg cctgctgtgg aggctgctgc tgcgctgctt gccgcacagg
    23761 cctgtgatgc ctggtattcc tccatggaaa cagcaccttt taactcctct gaaagtgtca
    23821 catagtctcc atgaacgtat gcgctgcttc cgctgtactg gatctgatac cagtcagcct
    23881 cttcgccgaa tacgctgact tcaccattct gtgtcagtgt tccaacctgc gcggaatctg
    23941 tggatgctgc ctcacggaca cgaaggctgt ccgcctgaac aactccggtg ataccgcaca
    24001 tattgcggta atgtgctttt gcctcttccc caaataccag atattcattc tttatgtagc
    24061 cttctacacc attggaagag atcttgctcc actcgtctcc tttttctgtt acagtaaccg
    24121 catagcccgg catcagaact ccgacgatct cggaatccac gcttgcctcg ctgcggatgt
    24181 tggcatatcc ttctacgttt gctgcagcca ttgtgctcca gtctgtttta tttaaaatgg
    24241 tttcttcctt tgtctctgct tttacgggtg cagccatgat aacagtcagt gtgagcacgc
    24301 tccccaggat gcccggcaca taactttttt tgaatgtcat acttacctcc tgaacaatta
    24361 ttacgttttt gttacatctt gttacaaacc gattctacca gtgttacatc tttttgtcaa
    24421 ctagaatatt tcgacaacat tcctgtcttt tcccatcgtt tttttcatag aacccacaat
    24481 ttttcaccac ttttccgctt ttttgacgaa ttggatcggt ttttattccc actttttctt
    24541 ctgcatgcgg cctgcctgga atacttttcc gtgtcttgtt tccttctttt tattttgctt
    24601 ttgtttcaca ttctgccctt tctgccctct ttttccgcaa atatttttta agaaattgtg
    24661 acagttcttt ttctattttt cgacatgcat tacaaacctt ttccactttt gacgcatttt
    24721 caaattttct tttaactttt tatttttaca tgatatttct ttctacatat ggtataatct
    24781 ttaaaaagaa agaaaattta tattttatca attatttttc tttcaattta tacgtgaaac
    24841 ttaccgcagg aaaataaaac agaccgacat tagaaaccga tcaggtgaaa ggaggatgtc
    24901 atatgttaca cagcacacag acaacgaaaa aacacacagg aaatttgatt ctgagattcg
    24961 gaatggcagg cctcctggcg gccggattat ttttttccgc tccgtattcg gcagcagccg
    25021 agacagttcc aagtgcagaa aatacagacg ccggtgaaat taccattcac ttcacccatt
    25081 acgatgcaca ggacctggaa ttctatctga acaccgatca ggcggccctt gcttttaccg
    25141 atgctgccct gacatttcag gattccacaa tggatgcgcc ggtcaccatg tcatatcttt
    25201 ccgatgatgg ttcctatgtc ctgtatcaga acagcgaacc ggtcgaggaa gtaaacggaa
    25261 cgttctccgt atcgatggtt acggaacctg gctacacact ggatatggat cagatttcct
    25321 tctatgttaa tggacaggcc gtttccaatg gcggttccgt tgtgattacg gattcttcgg
    25381 cggcgatgac ttctcttgtt tactgtaccg caatcccgga aacaccggca actgacccgg
    25441 agccatctct tccggaagag ccatctgcac cggaagttcc ggaagaaccg gccgctccag
    25501 aaccatccgt tccggaacag ccatccgcac cggcagaaac cccgaccgtg gtacagccat
    25561 ctgcttcgga acagccggca caggaaaata ttcttccgga aaccgcagct cctgcatcca
    25621 aaaccgcacc ggaacaggtt cctgcatccg aaccgatcgc agcagaaccg attgccagcg
    25681 cagttgttac agaagacgaa gaagttgatc tttccaccat ccacccggaa gttaccgcat
    25741 ccgctgttac ttcttccagc cagcttccag ccgcaacgca gattgtacca tctgtcagaa
    25801 caacagaccc ggatgcacag attattactt cggaaactgc agctcttgaa aatgccgcac
    25861 agaccgagaa tacacagact gcacacacag gaatcggctc ctggttccat cagcgaacaa
    25921 atcagatcct cgcagtcatg atcgctgtga tcctgcttct gatcctgatc gaatcacttc
    25981 tggcactaaa ggattccatg aatcagaaaa gatcgcatcg cttctattaa aaataaggtc
    26041 cataataaaa aataagcata caaaaagaag gaacgtatct catgaaacat gggatacgtt
    26101 ccttctctta tattattttt tatcctcttt ttttcgcagc cgctttctga atctcacaga
    26161 aatcgtgcag cgcctgacgg ccgattttta caattttacg gatgttgatg gaatttgttc
    26221 ctccctgacg cggcttgaag ctgatcggga tgaaacggat tttccggtga aaatacgctc
    26281 cgaaggcagt cagcagtgcg ttcggcagat tgtagttttc cggcatcagc ggcagatact
    26341 cggccacgta gtctgttttc atcagacgga acggggcatt tgcatccggc acggatacgt
    26401 ggaaataggt ccagagcacc gcgcacagca ctttttccac gacagcgcgg gaaaagccgt
    26461 cgccgcggct tgtgcggttt ccgaaaatgg cgtcgtagcc ctcgcggatc tgccagaact
    26521 tttcgaactc ctccggatcg gtctgaccgt cggaatcggt ctggaagata tagtccgcac
    26581 cgttttcaag tgcatagcgg tagccgtaca gaacggtgga accgtggccg ccgtttggct
    26641 tcgtcagcac ggtgagcaac gggtgggttt cgcactctgc tgaagcaatt tcatacgtat
    26701 cgtcgcggct tccgtcgttg atgatcacaa gacgggacgc accgccgccg ttatgcttct
    26761 ccacgacggg acaccattcc tcgatcgttc ttttgatatt ttccgactca ttataggctg
    26821 gtattacaat atataaggta tccattggta ttctccttgc atttccttcc atccattata
    26881 caggatttct tttcattgcg aaagatgcac tcatggagaa aatttcccag taccgttcgt
    26941 ttgttattct tcccacagtt tctgcggata acggccctcg tcttttaatt tctgcagtgc
    27001 tgccatgaca ttttctttgt cttctttgta cgttacgccg taccagttat cataggagcg
    27061 gagtacggtg acttccgctt ttttctcctg cagcagttca tcgacaacga acggcaggaa
    27121 gtattcgcct tttaacgggt tggttttgta tacattctcg aggaacggca ggaagcggcg
    27181 tttcagctcg atcagaatgc tcggtgtgaa gccccagagg ttcatggaaa cgatggttcc
    27241 ctgcggcaat ccggtccagg ttgcgccgtc atcctcggtg tattcggcct gtccgccgtg
    27301 gttttcgatt cttgtgcgct catgaatgcc gacaaggtgg ttctggtcgt ctgcggtgca
    27361 gatgccgcgg gctacgtggc cattgtcggt cagggttttg tacaggtcgt aggcaaccat
    27421 cgcgtactga taggtcgcgc cgtcctgctc ggttgtcagc tgacggtaga tctcctcgaa
    27481 tgccgttctt ccataataat catcggaatt gatgactgcg aacggtgcgc cgtcgatggc
    27541 ttctgccgca cagagaatcg catgaccggt tccccacggt ttgacgcggc cttccggcac
    27601 ttcgtagcct ttcgggagaa cgtcgagttc ctggtaaaca tactctacat ccatgtattt
    27661 ctcaattcgc ttgccgatgc cctcgcggaa tgcctcgtcg atggcttttt tgatgataaa
    27721 gatgactttc tcaaagcctg cctgttttgc atcataaatg gaaaagtcga taatcatatt
    27781 tccgtacggg tcaaccggat caatctgttt taatccgccg taacggcttc ccattcctgc
    27841 tgccatgatg actaatactg gttttttgct catgatgttc tctccttttt attctccaaa
    27901 ataaactttt aatctctccg caaagtctgc cgggatttct ctttcaaatg gaaatggttc
    27961 tgcatccgcc aaatactcgg cgatttcgcc caggacaccg gcgctccggt tcagtgacca
    28021 ggcggagatc cagtcgatcg tatcgtggca ggtatgcatg ccgaaaccat cgcggttcag
    28081 cgtcatcgcc gggatgccct tccatgcgaa ggtgttggaa tcgctgctcc agatgttgtt
    28141 gatgagcgat acgcctttgt ccgcctgttt taagtgctcc atgatcacgt cgcatgcctc
    28201 tttcgttgct gccacgccaa gcacggttcc gccgatggcc tgtccggcga ggtcgacgtt
    28261 catgttgaag cggtgctgcg ccagctcggc ttcatgcgct ttcacatacg cccggcttcc
    28321 gcagagaccc agctcctccg cgccgaacca gatgcattcc agtgtccgtt tcgggcggtt
    28381 ttcggcgaag tagtgtgcca gctccatgat gatcgctgcg cccgccatgt tgtcgtatgc
    28441 accaggtccc tgcggaacgg aatcgtaatg agccgtcagc gtcaggattt cctctggctg
    28501 ctcggttccc gggatgcgca ggatgatatt tttcgacgtt ctcctgattt ttttctgctg
    28561 taaaacgagg cgtgcacggc tcgctccccg ttctaccagt tccatcgcat caagataatg
    28621 aatgacggcg cacggcagtt tcggattttt tacgccgcgc agggtgcggt agtccggcag
    28681 acggtcctcg tctttgtcca gcggcgttcc ggtcaggatc agaaatccga cggctcctgc
    28741 tttttccagt ttttcatagt tttctgcatt ggcaccgccg ttgatcagga cgattttccc
    28801 ttctgcatgg gaaaggctga ctgggtcgcc gttttctacg tagaggaatg gtgcctccac
    28861 gccttccgga gcggtattgg ctgccgcgaa gcagccgcgg acggtgtatg ttttctcata
    28921 tggctctgtg accgtaaaga cagcttcgtc aacctgccat gtctcaatct cgaacggctc
    28981 gcggcggatc tgcaattcgc cctgccccca tttttctgcg atttcagaaa gttctttttc
    29041 gatccggcag gctgcttttt cttcgccttc cgttccggct tcgcggatgt agttaaactg
    29101 gctgacgaac gcatactgcc gctggccgct gatggtattt gctttcataa taatgttatg
    29161 tccttttctt ttcttttttt attttcttaa agttcttcca gtaccgctct cgcgctgcgc
    29221 acggctttga catcagtgcg gtagagggtg tattcgctta aggatggaag tcccatgttc
    29281 acattttctt tccggtactg catcgggctc gccagttcga tttttccgga catgtggtag
    29341 gcggtcacgc cggttttcgg atacagctcg cggatcgcat ccgcgcagat accgccgccc
    29401 gcctgaatac ggatgcgtcc cgccgcctgg cgctgcagtt cggcgagaag ctctgctcct
    29461 tttaccgcac tgttctgctg accggaggtg agaatggtgt tcataccgag cgataccgcc
    29521 tgctcaagcg cttctttcgg atcgcggcag acatcgaacg cgcggtgcag cgtcac
//
LOCUS       DAOMUF010000008        27027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_238_length_27027_cov_4.521949, whole genome shotgun sequence.
ACCESSION   DAOMUF010000008 DAOMUF010000000
VERSION     DAOMUF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 27027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27027
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238_length_27027_cov_4.521949"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1051
                     /locus_tag="PLN77_01320"
     CDS             398..1051
                     /locus_tag="PLN77_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42953.1"
                     /translation="MKEIIQGFYQKKHFWTRLILVIAAVILMGFALSWLLLVDLGTDP
                     CTLLNQTISTRLGISIGNWQALFNTVLLIFVIIFGGRNLGFGTLANMFLVGYSIDFFS
                     WIWAKVLPADLFTSMGVRIAVLIPALILFILAASFYMDVDMGTAPYDAVSFIISTHLP
                     NVSFRVIRIVYDFVVTAIAVLLGGKLGIVTVLMVITLGPVIEWLGNIMKQHLNIFDD"
     gene            1226..2071
                     /locus_tag="PLN77_01325"
     CDS             1226..2071
                     /locus_tag="PLN77_01325"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRM42954.1"
                     /translation="MSNTPNPVYEKLMKCYESFRSQIDFQPEVALILGSGLGDFANDI
                     RVTATLDYHDIEGFPVSTVPGHAGRFIFGYVGDVPVVCMQGRVHYYEGYPMTDVVLPT
                     RLMKLMGAKALFLTNAAGGIKQGTKPGSLMLLNGQIACFVPSPLIGPNIDELGTRFPD
                     MSQIYDLEFQKIARKAAASLDIDLMEGVYLQLTGPQYESPQEIAMCRTLGADAVGMST
                     ACEAIAARHMGMRVIGISCITNLAAGISPQPLCHAEVQEAADMVAPQFKKLVAATIQG
                     IAKTL"
     gene            2151..3461
                     /locus_tag="PLN77_01330"
     CDS             2151..3461
                     /locus_tag="PLN77_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRM42955.1"
                     /translation="MNTRFYNAKILVLDAENHFSIAEDELHVTGDTITYIGPGRKADP
                     DAAETPVFDREIDARGNLLIPGFKNAHTHTPMTFLRSYADDLPLHEWLERQVFPNEAK
                     LAGDDVYWLNILGIMEYLTSGITSNFDMYIQQKNSIAATVDTGFRTVLTSGLNNFVDS
                     PEILEEMYNYVNELSDRTSYLLGFHAEYTTGGPLLESVAKLAEKYHSPVWTHNAETKS
                     EVEGCKERWGLTPTQLMERLGMFQYGGGGYHCIWMEDRDFEIFRDRKLTAVTNPSSNL
                     KLASGICPLKRFYDNGINLAIGTDGPASNNCLDMFREMFLTTGLSKVREMDAAGIPAD
                     AILYMATAGGAHAMQLTDCDRLAAGKKADLVMIDLHQPNMQPENNLIKNLVYSGSKQN
                     VKLTMVNGQILYENGEFRIGFDPEEIYARSNAIIRRISGSHADN"
     gene            3567..4739
                     /locus_tag="PLN77_01335"
     CDS             3567..4739
                     /locus_tag="PLN77_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM42956.1"
                     /translation="MKKKVVSLLLAAAMTTAALAGCGSSNGSSSSDASTGADSTSATS
                     ASASTDGEKKDAAGEKIAMITDSGDITDESFNQITWETVCAYGDANGVEYEYYKPAED
                     TDEERINSIDLAVSEGATVVVMPGYLFGPAIAEEQDTYPDVTFIAVDVTEGDIVKNGK
                     AVDLGKNVYICSFEEEQAGYLAGYAAVKDGYTSLGFLGGIAVPAVIRFGYGYVQGINA
                     AAEEDGVDVNVKYYYGGQFYGDDSITARMEGWYADGTQVVFACGGGIWTSALDAAETH
                     DGKVIGVDVDQRSKIGERCLTSAMKGLPSAINNALDSYFSGNFSELGGTAQQLGLKDG
                     DYIGLPTDSESWGFTTFTQDQYETVLAGIRDGSIEISSDTENQPEVGSHVTVDYIS"
     gene            4914..6455
                     /locus_tag="PLN77_01340"
     CDS             4914..6455
                     /locus_tag="PLN77_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM42957.1"
                     /translation="MENEYIIEMLNITKEFPGIKANDNITLQLKKGEIHALLGENGAG
                     KSTLMSILFGLYQPTSGIIKKNGQEVHINTPNDANDLNIGMVHQHFKLVECFSVLDNI
                     ILGVEPTKGLFLEKKNARAKVMELSEKYGLMVDPDALISDITVGMQQRTEILKMLYRD
                     NEVLIFDEPTAVLTPQEIDELMKIMKNLAKEGKSILFITHKLNEIMEVADRCTILRKG
                     KYIGTVNISETSKEELSRMMVGRNVSFSVNKKPSKPGDTVLKVEHMTVPSKVHGNNAV
                     KDVSFNVRKGEIVCIAGIDGNGQSEFVQGLTGLEKVSGGKILFNGQDITKASIRDRSK
                     MGMSHIPEDRHKHGLVLDYSIEQNMVLQRYWQPEFESFGFIKSKAVREYAERLIEQYD
                     VRSGQGPVTITRSMSGGNQQKAIIAREIDKDPDLLVAVQPTRGLDVGAIEYIHSQLVA
                     QRDKGKAVLLVSLELDEVMDVSDRILVMYEGELVGQLDPKTVSVEELGLYMAGAKRDP
                     VPEEV"
     gene            6474..7595
                     /locus_tag="PLN77_01345"
     CDS             6474..7595
                     /locus_tag="PLN77_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM42958.1"
                     /translation="MNEKTKNALLSIVASLVCIVIGLLVGFIILYCINAENAVDGFTR
                     IIKGGFYLKPKGIGSEIAQSAPLIMTGLSVAFAFKTGLFNIGAAGQYTVGVFGALYFA
                     IILHMPWYVCLLAAMVCGAIWGAVPGIFKAYFNVNEVITSIMFNWIGLYLVNELMYNT
                     IPGMYDQKTTRTYKLSSASPKSLIPDCGMNSIFRTSSTTIAIFIAIAIAVIIYIVLNK
                     TTFGYELKACGFNKDAAKYAGINEKRNVVLSMTIAGALAGIGAGLYFLSGASEWNPQV
                     STALPAIGFNGIPVALLALSNPIGVIFAALFVAHISVGGAYLPTKYYQPEIADLIVAV
                     IIYLCAFVTLFKGIVANLFKSKKEQKANANADNGKEEKK"
     gene            7595..8530
                     /locus_tag="PLN77_01350"
     CDS             7595..8530
                     /locus_tag="PLN77_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM42959.1"
                     /translation="MFLLIKFTLLYAVVLMLVAMGGMFSERSGVINIALEGIMAIGGL
                     AGVFVLVLFPDLPAPALVALSILASAVSGMIYSLLLGFSAITLKADQTIGGTALNMLA
                     TAIAIVLVKAFNNGSDAGNGAASAKLNYTNAAFIYKIGPFTVNIFLFIGIILLICSYV
                     FLFKTKWGLRLRACGEHPQAADSVGINVYQWRYTGVIISGALGGIGGFAYIVPSVTTW
                     NFEVGVAGAGFLALAVMIFGQWKPFHIAGAAVFFAVFRSLANIADSTVLSHLGLSKNI
                     YSMMPFIASMIILAFTSKNSQAPKAEGIPYDKGAR"
     gene            8779..9420
                     /gene="deoC"
                     /locus_tag="PLN77_01355"
     CDS             8779..9420
                     /gene="deoC"
                     /locus_tag="PLN77_01355"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRM42960.1"
                     /translation="MLNTKLFDHTILKADATEAQVAKICDEALAHDFASVCVNQYYTK
                     FVAEKLKGSDVKVCTVVGFPLGMSDTRVKAFETRAAIEDGAQEVDMVINVGALKDGKY
                     DFVRSDIQTLKEVCGKDIVLKVIIETCLLTDEEKVKACELAKEAGADFVKTSTGFSTG
                     GAKAADVALMRKTVGPDMGVKASGGIHNADEAQAMVDAGASRLGTSATLAIIG"
     gene            9434..10063
                     /gene="udk"
                     /locus_tag="PLN77_01360"
     CDS             9434..10063
                     /gene="udk"
                     /locus_tag="PLN77_01360"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRM42961.1"
                     /translation="MDTILIGIAGGTGSGKTTLADKLVESFGRDEVSILRHDNYYKRH
                     DDIPFEERAKLNYDHPDAFDTDLLCQHILELKAGKSIEMPVYDYAMHNRSDKTILVHP
                     APVIVLEGILLFVEKDLCDLMDIKVFVDTDADVRILRRILRDVKERGRSLDSVVTQYL
                     TTVKPMHEQFVEPSKRRADVIIPEGGQNTVALEMLIERVRKHLNLVQNQ"
     gene            complement(10151..11029)
                     /locus_tag="PLN77_01365"
     CDS             complement(10151..11029)
                     /locus_tag="PLN77_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3881 family protein"
                     /protein_id="HRM42962.1"
                     /translation="MHAYLKTIGFSKIKTTKELQEILRDVVENYDRKKIVETEEHHLF
                     AEFSKEYAYDCGITVCGEIDENNRFQMEYYYPYFEGGQISSYEEVSVERHVDKESFAG
                     ACDDMRLGITMIFYLTHAAEYLEMKQKNALQPLPLPLSVSGLAAEGTILLPIQKDPEQ
                     VEKERRSTQQKNSLISAARNGDEDAMESLTMDDIDTYAMISQRLTKEDVFSIVDSFFM
                     PSGLECDQYSVMGEITEMNTTKNAVTGERVYELGLVCNDVPIDICINEKDLFGVPEVG
                     RRFKGQIWLQGMLEHL"
     gene            complement(11069..12358)
                     /gene="serS"
                     /locus_tag="PLN77_01370"
     CDS             complement(11069..12358)
                     /gene="serS"
                     /locus_tag="PLN77_01370"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRM42963.1"
                     /translation="MLDIKFLRENPEVVKQNIRNKFQDQKLELVDKVIELDKENRDIK
                     QEVEALRAKRNKISKQIGALMGQGKREEAEEVKKQVAVDGSRIEALSAREKEVEEEIK
                     KIMMVIPNIIDPSVPIGKDDSENVEAERFGEPVVPDFEIPYHTDIMESFDGIDLDAAR
                     EVAGNGFYYLMGDIARLHSAILSYARDFMIDRGFTYCVPPFMIHGNVVNGVMSFAEME
                     SMMYKIEGEDLYLIGTSEHSMIGKFIDSIIPEEKLPLTLTSYSPCFRKEKGAHGIEER
                     GVYRIHQFEKQEMVVVCKPEDSMMWYDKLWKNTVDLFRSMDIPVRTLECCSGDLADLK
                     VKSVDVEAWSPRQKKYFEVGSCSNLGDAQARRLKIRVNGEDGKKYLAHTLNNTVVAPP
                     RMLIAFLENNLNADGTVSIPEVLRMYMGGKEKLVPKH"
     gene            complement(12538..13053)
                     /locus_tag="PLN77_01375"
     CDS             complement(12538..13053)
                     /locus_tag="PLN77_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HRM42964.1"
                     /translation="MSDLFSKVGIDLGVVVLLLIVLIIVMMIMTVSMSLRLSRLNQKY
                     RSFMKGKDAQSLEKLFHQRFKEIDKLNKQNELTKKNLADLKKIQGMTLNKYGIVKYDA
                     FDDVGGKMSFALAMLDKENTGFILNAIHSRDNCFLYLKEIVKGESYIMLSAEEIDALR
                     QAITMNSIDLM"
     gene            complement(13065..13997)
                     /locus_tag="PLN77_01380"
     CDS             complement(13065..13997)
                     /locus_tag="PLN77_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRM42965.1"
                     /translation="MAARRGGLGKGLDSLISPGHSAKAEAKEKPAKPEVKVVEKVVEK
                     VVEKPVEMKLKISEIEPNREQPRKSFDEDGLIELSESIKQFGVLQPILVQKKDDYYEI
                     IAGERRWRAAKLAGIKEVPVIIKDFTEQEVVEISLIENIQRESLNPIEEAHAYKRLME
                     EFHLRQDEIAERVSKSRTAVTNSMRLLKLDDRVQQMVVDEMLTTGHVRTLLALENKEL
                     QYNTAMKIFDEKLSVRETERLVKEILNPKVKKEKKVNLEEEAIYEGLEEKIKSIIGTK
                     VSIHRGAKHKGKIEIEYYSQEELERIMDLFESIQ"
     gene            complement(13997..14767)
                     /locus_tag="PLN77_01385"
     CDS             complement(13997..14767)
                     /locus_tag="PLN77_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM42966.1"
                     /translation="MGRTIAIANQKGGVGKSTTAINLSSCLGEMGQKVLTIDMDPQGN
                     TTSGMGVEKNEVENTIYELLLGESKLEDCIIPLNFDNLSLIPSNVNLAGAEIELIGVE
                     DKEFILKNAIDQVRDQYDFIIIDCPPSLNMLTINAMTTADTVLVPIQCEYYALEGLSQ
                     LMHTIELVQERLNPELEMEGVVFTMYDARTNLSLQVVENVKDSLDQNIYKTIIPRNVR
                     LAEAPSYGMPINYYDKRSTGAESYRLLADEVIHRGDEE"
     gene            complement(15891..16016)
                     /locus_tag="PLN77_01390"
     CDS             complement(15891..16016)
                     /locus_tag="PLN77_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42967.1"
                     /translation="MEEAEKAKKDKNELRKGKKRLKRGKCSIDMGEKNKNGRERQ"
     gene            16774..17736
                     /locus_tag="PLN77_01395"
     CDS             16774..17736
                     /locus_tag="PLN77_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM42968.1"
                     /translation="MKHSKKLVTLSVLTTMSGAAIYFLNKTLDTAAVRKNLLASAEKE
                     IFSWQFGDIFYTKKGTGTPMLLLHDLHCASSGREWQYIEDALAQDHTVYTLDLLGCGR
                     SDKPAITYTNFLYVQLIVTFIKQVIGCPTDVIASGLSGSFVVTACNTAPKCFGRIMMI
                     NPTDLAKLNKIPDKRSRFLKRMIELPLVGTLLYHTLTGRSNIELLFTESYYHNPFHRS
                     PEDVDAFYESAHLQSSAGKYLFASLAGNYVNLNIGHALKHIDNSIFLIGGEEEPGIAE
                     TFALYTALNTSIETQILPKTKHMPQMEAPQALLDQIQIFFAPVQ"
     gene            complement(17835..18629)
                     /locus_tag="PLN77_01400"
     CDS             complement(17835..18629)
                     /locus_tag="PLN77_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42969.1"
                     /translation="MTVLCILLVGCGNAETAETEEKMRFENLDPAKVNMQYGGLKEWD
                     RFYNSFYEQKEGSDLIVLGTVEDYSCFAGGIEIATNISLRVDDVLKGDMEAGEHITVR
                     KPGGAVTVEEYLQSMEDAGITYWNAEELKAEYSEEERRENYIQISFCDLDPVIGQKSL
                     YFLEKDAEKELYYRLCDGFGQYVETASGEYVNAYEIADEKRNADEPMMLALGETVEFD
                     PDAAPEERINIYTMDEIKEGMETHTAPPTDYPGAEEDALEMDCEPG"
     gene            complement(18791..20377)
                     /locus_tag="PLN77_01405"
     CDS             complement(18791..20377)
                     /locus_tag="PLN77_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRM42970.1"
                     /translation="MKKKGKMAACLILLVLLLAVLVVLTKKNSTADADSSSSDSGADA
                     EKVVDFSKDDVTALSFQINGETVSFTKTAGDDDTDIWTYDQEDGFPLDEGKITSVLSS
                     LSSMTAERVIEGDEIDSMADFGLETPSQEVVVTAGDEKTTIHVGDKNSSSRYYIYLND
                     DTSKVYLVSTSLGTMFPSDMMEWATTESMPSVTAENITKLQVEGENGYTLTKEVSAAD
                     SALQTDEWQVVDADGAAHGGDADSIGTMTSAVASLSFGDLVTYNASDLSQYGLDQPKT
                     TIRVHYTEEQEVEADDTTTADTSSDSTADSSSSDSTATSSSSETTTVTVEKDLVLYVG
                     NANEDGGSYYVKLDGSNEVHLMTASNVETFTGKKASDFWNMYIGMENVSDLTSLDITY
                     NGETKTYVRHMEEKKDDDSDSTTQEISYICGDETIDKSTFTTFYSSLIGLKAQTKDES
                     LVQAKDAEFTAVFHMTDGDLTFAYSPYDSSFYYTVDEDGVPSLVNKNNVKTMVENYGK
                     LLAAKTEDDSSSDSTSESAE"
     gene            complement(20377..21894)
                     /locus_tag="PLN77_01410"
     CDS             complement(20377..21894)
                     /locus_tag="PLN77_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HRM42971.1"
                     /translation="MSSEKKEKKQHKMNFNIKEKLKKTFSKEHLKEVFTGEEQKKHLK
                     QGSYSSIMTVIVLAVIVVINLIVAQIPTSKTQIDLSTQKMYSITDETKTALKDLDQDV
                     TLYYIVQKGSEDDTIEKLLEHYDELSDHIKVETKDPDLYPKFTSQYTDQTVAANSVIV
                     VCGDKSKVVSNSDMWETSTNYQTYQTQTTGFDGEGQITSAITYVTSENNPKVYWVTGH
                     GEASESDLSTNFSDAINKNNVEISDLALMTDDIPEDAEELVIMSPTTDFSEDEANKVI
                     TYLENGGKLLMFTSYTGTDMPNLDSILENYGVKRSSGIVVETDSQHYYPQMPYYLLPN
                     IQSDDITTEVKSNYILMPVAQAIQKLDSYRDTITIKSLLTTTEDAYIENDPENSTWSK
                     SADSETGAFDLGVSITETVDDKETQIIYFSSASMLSSQIDQAISGANSKLAATALTSM
                     CDVEQTVVIPSKSTSYTNLVFTQGAVNTWSIITIAGIPLVLVVIGVMIWMKRRKQ"
     gene            complement(21907..22770)
                     /locus_tag="PLN77_01415"
     CDS             complement(21907..22770)
                     /locus_tag="PLN77_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM42972.1"
                     /translation="MTAIYKKELKSYLTSMIGYLFMAFTLALFGLYFTAINLQQGYPE
                     IGYALQNSAFILLIAVPVLTMRVLSEEQKNKTDQLLLTAPVKISDIILGKYLALLTIY
                     VIPVLIMCLYPLLLGTHGTVSYAVSYTSILGYFLLGAAYISVGVFVSSITESQVIAAV
                     VGFVILFLCYVESGIANFFPEGAGSSFFAFFIIIALVCLWIGSMIKNPIITGVIAVIG
                     EGALTAVYFTKSTLLEGKIQDLLGVFNMAGHMDNFVNGILDIGGVVYYLSVIAICTFL
                     AMQSLQKKRWN"
     gene            complement(22767..23876)
                     /locus_tag="PLN77_01420"
     CDS             complement(22767..23876)
                     /locus_tag="PLN77_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM42973.1"
                     /translation="MIEVKNLVKRYGDHVAVNNLSFTVEEGQIYGFLGPNGAGKSTTM
                     NIITGYLASTEGEVLINGHNILEEPEEAKKCIGYLPELPPVYTDMTVLEYLKFVAELK
                     KIPKDQRKKQILEVMNLVKITDMQNRLIKNLSKGYRQRVGLAQAVLGYPPIIILDEPT
                     VGLDPKQIIEIRDLIKSLGKKHTVILSSHILSEVSAVCDYVMIIAKGQLVASDTPENL
                     SKLMLGSNTVTCTVHGTRKQLVPALNALDAKEIQYENTPNEEQIKVKITTEKDKDIRE
                     ELFYALAKANCPILELTSSSMSLEDVFLELTDDSGKAARKLKKAEKEQLKQKECEKEC
                     EPEADKTAVGETSEEKSTDAAESAAETKQEEGDAQ"
     gene            24187..25533
                     /locus_tag="PLN77_01425"
     CDS             24187..25533
                     /locus_tag="PLN77_01425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRM42974.1"
                     /translation="MNKKDYYWAKYMLIFGFFCICFGTSIFLKTEHAPDEAMRMLIPE
                     YIVSHHTLPNGMEESVRHPLWGFSYALYPYLTAIISSVFMAITSLFTKSAAALLTAAR
                     LTSVLSGTGTLIVVFLIGEELFERRESALLGGIFVGFLPQFVFLSCYVNNDSFAVFTV
                     ALIIYFWIRGMKSAFCKKDCIGLGAGCGLCALSYYNAYAYLLCSILLFFALMIHFRKP
                     AKEIFVKALAVFAIAFLIGGWFFIRNAVIHDGDLLGMRTSNESASLYAADEYKPENRQ
                     TPASEGWSFQQTYLQTPEGRTSNWLFSTVSSFIGSFSYMTVHLPMVLYLLYGALMAFG
                     FLVFLFLGMVPHWFRKKPQLLLFVMLFLACLVTLFMDMYNTYFSDYQSQGRYLMPALV
                     PLMIWIDDGYSSLTAKLPAEWKRASCHLTLLPGTIWLVLFLISYIGFLIPGCCMAS"
     gene            complement(25617..26342)
                     /gene="rsmG"
                     /locus_tag="PLN77_01430"
     CDS             complement(25617..26342)
                     /gene="rsmG"
                     /locus_tag="PLN77_01430"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRM42975.1"
                     /translation="MKEYNLEGFERGLFELGITLSEEQKTQFLQYYELLVEWNSFMNL
                     TAITEFDQVITKHFLDSLSLVKVCDVAQAGRILDVGTGAGFPGIPLKIAFPEVEVVLL
                     DSLNKRVKFLNEVIGQLGLSKITAIHGRAEDFARQKEYREQFDLVVSRAVANLSSLSE
                     YCLPYVKVGGRFVSYKSGKLSEELTAAGKAIHVLGGEAKEPVYFQLTGTEDERSFVCI
                     EKGKATPKKYPRKAGTPAKEPIQ"
     gene            complement(26467..>27027)
                     /locus_tag="PLN77_01435"
     CDS             complement(26467..>27027)
                     /locus_tag="PLN77_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRM42976.1"
                     /translation="RLLLRQDNADLRLRKYGYRVGLISEEQYAALKRKEQQIQEEIER
                     VENTYVGTSSNVNELLAEYGSTLLSGGSSLAELIRRPELNYKMLAEVDPKRPKLPEDV
                     QEQVNINIKYDGYIKRQMKQVEQFKKMEEKKIPENINYDEIQSLRIEAKQKLNLYRPI
                     NIGQASRISGVSPADISVLLVYLGHK"
BASE COUNT         6337 a         7233 c         5813 g         7644 t
ORIGIN      
        1 gtatattgta gtttttttca aggtcctgtc cgtctcgcgt ggacacattg tagaatgcac
       61 aaatttcatt cagctcacat gttcatttcg ttaatttatt aatgaacgtg ttttttcaat
      121 tttatgtttt atgttaattt atgttcattt atcgttcatc attttgtgaa catcaagttg
      181 acatcattaa aagaaaatag tttacatttt gggaaaaata tactaaaata acagaaacgg
      241 tcaaatctcc gccgaaaaaa aacggcggag atttttctgg aaagaagggc gaaaaacgtg
      301 ttggaaagac gatcgaaaaa tttcgaagaa aatgcgtaga aaatccgccc gaaaaggtga
      361 aaaaccaagc ataaatcaca gggaggaact tcatactatg aaagaaatca tccagggctt
      421 ctaccagaag aaacatttct ggacccgcct gattctggtc atcgctgccg tcatcctgat
      481 gggctttgcg ctctcatggc tgcttctcgt cgatctcggc accgatccgt gtaccttgtt
      541 aaaccagacc atctccaccc gcctcggcat cagcatcgga aactggcagg cgctcttcaa
      601 taccgtcctg ctcatcttcg ttatcatttt cggcggcaga aacttaggct tcggaaccct
      661 cgcaaacatg tttctcgtgg gatattccat cgactttttc tcctggatct gggcgaaagt
      721 ccttccggca gacctcttca cctcgatggg tgtgcgaatc gccgtgctga tcccggcgct
      781 gatcctgttt atccttgccg catccttcta tatggatgtt gatatgggaa ctgcaccgta
      841 cgacgcagta tccttcatta tctccaccca cctgccgaac gtttcgttcc gtgtgatccg
      901 catcgtctac gacttcgttg tcaccgcgat cgccgttctg ctcggcggaa aactcggcat
      961 tgtcaccgtg ctgatggtta ttaccctcgg tccggtcatt gaatggctgg gaaatatcat
     1021 gaaacagcat ctgaatattt ttgatgactg aaatcattgc ttttccgcgc tttctatact
     1081 acaatagaac taccagatga tcttttttct aaaagaaaag cctgcctccg ggactcacat
     1141 ctctgtggca ggcttttctt ccctaaaaat agccgaaaaa agactcaaaa agcagaaaat
     1201 catttatatt aaggaggttt ttcctatgag caacacccca aaccctgttt acgaaaaact
     1261 gatgaaatgc tacgagagct tccgctccca gatcgatttc cagccggaag tcgcactgat
     1321 tctcggttcc ggcctcggtg attttgcgaa tgatatccgc gttaccgcaa ccctcgatta
     1381 ccacgatatc gaaggcttcc cggtttccac tgttccgggc cacgcgggcc gttttatctt
     1441 cggctacgtc ggcgatgtgc cggttgtctg catgcagggc cgcgttcatt attatgaagg
     1501 ctatccgatg accgacgtcg ttctcccgac ccgcctcatg aagctgatgg gcgcaaaagc
     1561 actgttctta accaacgccg ccggcggcat caaacagggc acaaaaccag gctctctgat
     1621 gctgttaaac ggccagatcg cctgcttcgt tccatctccg cttatcggac cgaacatcga
     1681 cgagctcggc acccgtttcc cggatatgag ccagatctac gaccttgaat tccagaaaat
     1741 cgcacggaaa gccgcagcca gccttgatat cgacctgatg gaaggcgttt atctgcagct
     1801 caccggcccg cagtacgagt ccccgcagga aatcgccatg tgccgcaccc tcggcgcaga
     1861 cgcggtcggc atgagcaccg cctgcgaagc catcgctgca cgccatatgg gcatgcgcgt
     1921 catcggtatt tcctgcatta ccaatcttgc cgccggcatc tccccgcagc ctctgtgcca
     1981 tgcggaagtt caggaggcag ctgacatggt tgcaccgcag ttcaaaaaac tcgttgccgc
     2041 aacgattcag ggcatcgcaa agacactgta aacacacagc ggccggggtc tgtcaaaaaa
     2101 acaggccccg gccgctgaaa acagcacgta aaataaagga gagatctttt ttgaatacac
     2161 gtttttacaa cgccaaaatt ctcgtactcg acgctgaaaa ccatttttcc atcgccgaag
     2221 acgaacttca tgtcaccggc gacaccatca cctatatcgg acctggtaga aaagcagatc
     2281 cagatgccgc agagacccct gtcttcgacc gcgaaattga cgcccgcggc aatctcctga
     2341 tcccgggctt taaaaacgca cacacgcaca ccccgatgac cttcctccgc tcctacgcgg
     2401 acgatctccc gctccacgaa tggctcgagc gccaggtctt cccgaacgag gcaaagctcg
     2461 ccggggacga cgtttactgg ctcaatatcc tcggcatcat ggaatatctg acgagcggca
     2521 tcacctccaa tttcgacatg tacatccagc agaaaaacag catcgctgcc acggtcgaca
     2581 ccggattccg caccgttctt accagcggtc tcaacaactt cgtcgacagc ccggaaattc
     2641 tcgaagagat gtataactat gtcaacgaac tctccgaccg cacctcgtat ctgctcggtt
     2701 tccacgccga atatacgacc ggcggcccgc ttcttgaatc cgttgccaaa ctcgccgaaa
     2761 aataccattc ccctgtctgg acacacaacg ccgaaacaaa aagcgaagtc gagggctgta
     2821 aagagcgctg gggactcacc ccgacacagc tgatggaacg cttaggcatg ttccagtacg
     2881 gaggcggcgg ctaccactgc atctggatgg aggaccgcga tttcgaaatt ttccgcgacc
     2941 ggaaactgac ggccgttacc aatccctcct caaacctgaa gctcgcctcc ggcatctgcc
     3001 cgctgaagcg tttttacgat aacggcatca acctcgccat cggcactgac gggccggcca
     3061 gtaacaactg cctcgatatg ttccgcgaaa tgttcctgac caccggtctt tccaaagtcc
     3121 gtgagatgga cgccgcagga atcccggctg atgcgatact ctacatggca accgcaggcg
     3181 gcgcgcacgc catgcagctt actgactgcg accgtctcgc tgccggcaaa aaggccgatc
     3241 tcgtcatgat tgacctccat cagccgaaca tgcagccgga gaataatctg atcaaaaacc
     3301 tcgtttacag cggcagcaaa cagaacgtaa agcttaccat ggtaaacggt caaatcctct
     3361 acgaaaacgg cgaattccgc atcggctttg acccggaaga aatttatgca agatccaacg
     3421 ccattatccg aagaatttcc ggctcgcatg cagataatta gtggatttca cgcagttttt
     3481 cctttgcttg acatactttt ttcttttata ttataatgta gccatacacg caaaaacgct
     3541 gtacctcttg aaaggagaat actattatga aaaagaaagt agttagctta ctgcttgcag
     3601 ccgccatgac aactgctgca ctcgcaggat gcggttcctc caacggctcc tcttcctccg
     3661 acgcatccac aggcgccgat tccaccagcg caacttccgc ttctgcttcc acagatggag
     3721 aaaaaaaaga cgccgcaggt gagaaaattg ccatgatcac agactccgga gatatcaccg
     3781 acgaatcctt caaccagatc acctgggaaa ctgtatgcgc ctacggcgat gcaaacggcg
     3841 tagagtacga gtactacaaa ccggcagagg ataccgatga agagcgtatc aactccatcg
     3901 atcttgctgt ttccgagggt gctaccgttg ttgtaatgcc aggttacctg ttcggacctg
     3961 ccatcgctga agagcaggac acctatccgg atgtaacctt catcgcagtc gatgttaccg
     4021 aaggtgatat tgtaaaaaac ggcaaagctg ttgatctcgg aaagaacgtg tacatctgct
     4081 ccttcgaaga agagcaggcc ggctacctgg ctggatacgc tgcagtaaaa gacggctaca
     4141 caagtcttgg tttcctcggc ggtatcgctg ttccggccgt tatccgtttc ggttacggtt
     4201 atgtacaggg tatcaacgct gctgctgaag aagacggcgt agatgtaaac gtaaaatatt
     4261 actatggcgg ccagttctac ggcgatgact ccatcaccgc ccgtatggag ggctggtatg
     4321 cagacggaac acaggttgta ttcgcctgcg gcggcggtat ctggacatct gctctggatg
     4381 cagctgagac acacgacgga aaagttattg gtgtagacgt tgaccagcgt tccaaaatcg
     4441 gcgagcgctg cctgacttcc gctatgaaag gtcttccaag cgccatcaac aacgctctgg
     4501 attcctactt cagcggaaac ttctctgaac ttggcggaac tgctcagcag cttggactga
     4561 aagacggaga ttacatcggc cttcctaccg attccgaatc ctggggattc acaaccttca
     4621 cacaggatca gtatgaaact gtactggctg gtatccgcga tggttccatc gaaatctcca
     4681 gcgatacaga aaatcagccg gaagtaggtt ctcacgtaac cgttgattac atcagctaat
     4741 gtgcatactg tttctgtaac aatatgcctt ttggggaaga gaatattatt tctcttcccc
     4801 tttttttctg tcacacaaaa tgctatacta gacataatga aagcccgcag ttctgccctg
     4861 ttttccatgc cttcggcagt cctgcaaaac cacgcttgaa agggaatatg tatatggaaa
     4921 atgagtacat tattgagatg ttgaacatca ccaaagaatt cccgggtatc aaggcgaacg
     4981 acaacattac gttacagctc aaaaaaggag aaatccatgc tctgcttgga gaaaacggtg
     5041 caggaaaatc aaccctgatg agtattcttt ttggtctgta tcagcctacc tccggaatca
     5101 tcaagaagaa tgggcaggaa gtccatatca acacgccgaa cgacgccaat gatctcaaca
     5161 tcggtatggt gcaccagcac tttaagctgg tcgaatgttt ctcggttctc gacaacatca
     5221 tcctcggcgt agagccgacc aaaggccttt tcctcgagaa aaagaatgcc cgtgcaaaag
     5281 tcatggagct gagcgagaaa tacggtctga tggtcgatcc ggacgctctg atctcggata
     5341 tcaccgtcgg catgcagcag cgtaccgaaa ttctgaaaat gctctaccgt gacaacgaag
     5401 tcctgatctt cgacgagccg accgcggttc tgacgccgca ggaaatcgac gagctgatga
     5461 agatcatgaa gaacctcgca aaagaaggaa aatccatcct cttcatcacc cataaactca
     5521 acgagatcat ggaagttgcc gaccgctgta cgattctccg caaaggaaaa tacatcggaa
     5581 ccgtcaacat cagcgaaact tccaaagaag agctctcccg catgatggtc ggaagaaacg
     5641 tcagcttctc cgtcaacaaa aagccgtcaa aaccgggcga caccgttctg aaagtagagc
     5701 acatgaccgt accgagcaag gtgcacggaa acaacgccgt caaagatgtt tcctttaatg
     5761 taagaaaagg cgagattgtc tgcatcgcag gtatcgatgg aaacggccag tccgagttcg
     5821 ttcagggcct cacgggactg gaaaaagtat ccggcggtaa aattctgttc aacggacagg
     5881 acatcacgaa agcttccatc cgcgaccgct ccaagatggg catgagccac atcccggagg
     5941 accgccataa acacggcctc gtgctggatt actccatcga gcagaacatg gtgctgcagc
     6001 gttactggca gccggaattt gaatccttcg gctttatcaa atcaaaagcc gtgcgcgaat
     6061 atgccgagcg cctgatcgag cagtacgatg tccgcagcgg ccagggtcct gtgaccatca
     6121 cccgcagcat gtccggcgga aaccagcaga aagcgatcat cgcgcgtgag atcgacaaag
     6181 atcctgatct tctcgttgcg gtacagccta cccgtggtct tgatgtcgga gccatcgaat
     6241 acattcacag ccagctcgtt gcacagcgcg acaagggaaa agccgttctt ctggtttctc
     6301 tggaactgga tgaggttatg gatgtttccg accgtattct tgttatgtac gaaggagagc
     6361 tggtcggcca gctggatccg aaaaccgttt ctgtcgaaga actcggtctc tacatggcag
     6421 gtgcaaaacg ggatccggtt cccgaagaag tataaaagag aggtaagaaa agcatgaacg
     6481 aaaaaacgaa aaacgctctt ctgtccattg tcgcttctct tgtatgtatt gtgatcggtc
     6541 ttcttgtggg atttatcatt ttatactgca tcaacgcgga gaacgcagtg gatggattta
     6601 cacgaatcat caaaggcggc ttttacttaa agccgaaagg aatcggaagt gaaatcgctc
     6661 agtccgctcc cctgattatg accggacttt cggttgcatt tgcttttaaa acaggtctgt
     6721 tcaacatcgg tgctgccgga cagtacaccg tcggtgtctt cggcgccctg tattttgcca
     6781 tcatcctcca catgccatgg tatgtatgcc ttctggctgc tatggtctgc ggagccatct
     6841 ggggagcagt tcctggtatc ttcaaagcat acttcaacgt aaatgaagtt attacttcca
     6901 tcatgttcaa ctggattggt ctttatcttg tcaatgagct gatgtacaac accatcccgg
     6961 gcatgtatga tcagaagacc acacgtactt acaaattaag ctccgcttct ccgaaatccc
     7021 tgattccgga ctgcggcatg aactccattt tccgtaccag ttccacaacg attgcgattt
     7081 tcattgcgat tgccattgcg gtcatcatct acatcgttct gaacaaaaca accttcggct
     7141 atgagctgaa agcctgcgga ttcaataagg atgctgccaa atacgccggc atcaacgaga
     7201 agagaaacgt tgttctctcc atgaccatcg caggtgcgct tgccggaatc ggtgcaggcc
     7261 tctacttcct ctccggtgca tccgagtgga acccgcaggt atcgacagca ctcccggcca
     7321 tcggatttaa tggtatcccg gttgcgcttc ttgcacttag caacccgatc ggcgttatct
     7381 tcgctgctct cttcgttgct catatttcgg tcggcggtgc ttacctgcct acgaaatatt
     7441 atcagccgga gatcgcggac ctgatcgttg ccgttatcat ttacctttgc gcattcgtta
     7501 ccctgttcaa gggcatcgtt gcaaacctgt tcaagagcaa aaaagagcag aaagcaaatg
     7561 ccaatgcaga caacggaaag gaggagaaga aataatgttc cttttaatta aatttacctt
     7621 actgtacgcc gttgttctga tgctcgttgc gatgggcggt atgttctccg agcgaagcgg
     7681 tgtcatcaac atcgcgctgg aaggaatcat ggcgatcggc ggccttgccg gtgtcttcgt
     7741 cctggttctg ttcccggatc tcccggctcc tgcacttgtg gcactttcca ttctggcgag
     7801 cgccgtatcc ggtatgatct actctctgct gcttggcttc tccgccatca cactgaaagc
     7861 agaccagacg atcggcggaa ccgcgctgaa catgctggct actgccattg ccatcgtcct
     7921 ggtaaaagca ttcaacaacg gctcggatgc cggaaacggt gcagcttccg caaagctgaa
     7981 ctacaccaac gccgctttca tttacaagat cggaccattt accgtgaaca tcttcctgtt
     8041 catcggcatc atccttctga tctgcagcta cgtgttcctc ttcaaaacga aatggggact
     8101 gcgcctgcgt gcctgcggtg agcatccaca ggcagccgac tctgttggta tcaacgtata
     8161 ccagtggaga tacaccggtg ttatcatctc cggtgcactc ggcggtatcg gcggatttgc
     8221 atacatcgtt ccgtccgtta ccacctggaa cttcgaagtc ggcgtagccg gtgccggatt
     8281 ccttgccctc gctgttatga tcttcggtca gtggaaaccg ttccacattg ccggagcagc
     8341 cgtattcttt gcggtattcc gttccctggc aaacattgcg gactccaccg tcctctccca
     8401 tctgggactg tccaagaaca tttacagcat gatgccgttt atcgcatcca tgattattct
     8461 cgccttcaca tccaagaatt cccaggctcc gaaagccgaa ggtattccgt acgataaggg
     8521 cgcaagataa tatgtaacta ttcagagcca tgtgaaatgg aagaaaaaat tgcgaatcat
     8581 gcttgcatga atgagcggtt ttttctggaa tttcatatgg cgagcagcca catcgagatg
     8641 cagcgcagcg gaatcgagat gtggctctga atagttacaa tttttaaagg cagagccgtt
     8701 aaatccgaac tgtgtttttt cggctctgcc ttttctctat cattctgatt tatttttcat
     8761 caaagaaagg aaaaaattat gctgaacacc aaattatttg accacactat cttaaaagca
     8821 gacgcaaccg aagcacaggt tgcaaagatc tgcgacgagg cacttgcaca tgattttgct
     8881 tctgtctgcg tcaaccagta ctacaccaaa ttcgttgctg aaaagctcaa aggatctgac
     8941 gtcaaagtct gcaccgttgt cggcttccct ctgggcatgt ccgatacccg tgtaaaagca
     9001 tttgagaccc gtgcggccat cgaagacggt gcacaggagg ttgatatggt catcaacgtc
     9061 ggagccctca aagacggcaa atacgacttc gtacgctccg atatccagac cttaaaagaa
     9121 gtatgcggca aggacatcgt tctgaaagtc atcatcgaga cctgcctttt aaccgatgaa
     9181 gagaaagtaa aagcgtgcga gctcgcaaaa gaagccggcg ctgacttcgt aaaaacctcc
     9241 accggatttt ctaccggcgg tgcaaaagcg gctgatgttg ccctgatgag aaagaccgta
     9301 ggcccggata tgggcgtaaa agcttccggc ggaatccaca acgcagacga ggcacaggct
     9361 atggtagatg ccggcgcaag ccgtctcgga acaagcgcaa cactggctat catcggataa
     9421 aaaggagttt cttatggata cgatactgat aggaattgcc ggaggaaccg gcagtggaaa
     9481 aacgacactg gcagataagc tggtagaaag ttttggacgc gatgaagtga gcattctgcg
     9541 ccatgacaac tattacaaac gccatgacga tattcctttt gaggagcgtg ccaaattaaa
     9601 ttacgatcat cccgatgcct ttgataccga cctgttatgc cagcacattc tcgagctgaa
     9661 agccggaaaa tccatcgaga tgccggttta cgattatgcc atgcataacc gctcggataa
     9721 aacgattctg gtccatcctg ccccggttat cgtgctcgag ggcattctcc tcttcgttga
     9781 gaaggatctc tgcgatctga tggacatcaa agtctttgtc gataccgatg cggacgtgcg
     9841 gattctccgc cggatcctgc gcgatgtaaa agagcgcggc cgttccctgg acagcgttgt
     9901 aacgcagtac ctgacgaccg taaaaccgat gcacgagcag ttcgtagagc cgagcaagcg
     9961 ccgtgccgac gtgatcatcc cggagggcgg acagaacacc gttgcgcttg aaatgctgat
    10021 cgagcgggtg cgtaagcatc tgaatctggt gcagaaccag taaacatccg tttatcgtta
    10081 taaaaatctg caaaaaaaga acgaggactg aattttctct ttttccgtcc tcgctctttt
    10141 tatttttccc ttacagatgt tccagcattc cctgcagcca gatctggcct ttgaaacgtc
    10201 ttcccacttc cggtactcca aataaatcct tttcgttgat acagatgtct atcggcacat
    10261 cgttgcagac gaggccgagc tcatacaccc gctcgcccgt taccgcgttc ttcgttgtat
    10321 tcatctccgt aatttcaccc atcacgctgt actggtcaca ttcgagaccg ctcggcatga
    10381 aaaagctatc tacaatagaa aagacatctt ccttcgtcag acgctgggaa atcatggcat
    10441 atgtgtcaat atcatccatg gtgagacttt ccatcgcatc ctcatccccg tttctggcag
    10501 ccgagatcag tgaattcttc tgctgcgtgc tgcggcgctc cttctcgacc tgttccgggt
    10561 ctttctggat cggaagcaga attgttcctt ccgctgcaag accggagaca gaaagcggga
    10621 gcggaagcgg ctgcagcgca tttttctgtt tcatctcaag atattccgct gcatgcgtca
    10681 gataaaagat catcgtgatg ccaagccgca tatcgtcgca ggcgcctgca aacgattcct
    10741 tatcgacgtg gcgctccacc gatacctcct cgtaggagct gatctgcccg ccctcgaaat
    10801 agggataata atattccatc tggaaacggt tgttctcatc aatttcccca cagaccgtga
    10861 taccacagtc atatgcatat tctttcgaaa attcagcaaa aaggtggtgt tcttctgttt
    10921 ccacaatctt tttacggtcg tagttttcta ctacatcacg aagaatctcc tggagttcct
    10981 tcgtcgtctt gatctttgaa aatccaatgg ttttcaggta agcgtgcata agaacacctc
    11041 ctctcattcc tttgtatttc ttatgcgatt agtgtttcgg aaccagtttc tcttttccgc
    11101 ccatgtacat acgaagaact tccggaatgc ttacggttcc gtctgcattc aggttgttct
    11161 ccaggaatgc gatcagcata cgcggcggag caactacggt gttgttcagg gtatgtgcca
    11221 ggtatttctt tccgtcctcg ccgttgacac ggattttcag acgtcttgcc tgtgcatcac
    11281 caaggttgga gcagcttcct acctcgaagt atttcttctg acgcggagac catgcctcaa
    11341 catcgacaga ttttactttc aggtcagcca ggtcaccgga gcagcactcc agagtacgaa
    11401 ccgggatatc catggaacgg aacaggtcta cggtgttctt ccacagtttg tcataccaca
    11461 tcatgctgtc ttccggtttg cagacaacaa ccatctcctg tttctcgaac tgatggatac
    11521 gataaacacc acgctcctcg atgccgtgtg ctcctttttc tttacggaag cacggagagt
    11581 agctggtcag ggtcagaggc agtttctcct ccgggatgat gctgtcgatg aatttaccga
    11641 tcatggagtg ctcgctggtt ccgatcaggt acagatcctc gccctcgatt ttgtacatca
    11701 tggactccat ctcagcgaag ctcataacac cgtttacgac gttgccgtgg atcatgaaag
    11761 gcggtacgca gtaggtaaat ccgcggtcga tcatgaaatc tcttgcataa gaaaggatgg
    11821 cagagtggag gcgtgcaatg tcgcccatca gataatagaa tccgtttcct gcaacttctc
    11881 ttgctgcatc cagatcgatt ccgtcgaagg actccataat gtcggtgtga tacgggattt
    11941 caaaatccgg tacaaccggc tcgccgaagc gctctgcttc tacgttctcg ctgtcatctt
    12001 ttccgatcgg tacggaagga tcgatgatgt tcgggatcac catcatgatc ttcttgattt
    12061 cttcttctac ttctttttct cttgcagaaa gagcctcgat gcgggaaccg tcaacagcta
    12121 cctgtttctt tacttcctca gcctcttctc tcttgccctg gcccatcagt gcgccgatct
    12181 gtttggagat tttgtttctt tttgcacgaa gtgcttctac ttcctgtttg atgtcacggt
    12241 tttctttgtc cagctcgatg actttatcaa ccagctccag tttctgatcc tggaatttat
    12301 ttcggatatt ctgttttaca acttccgggt tttctcttaa aaacttaatg tctaacataa
    12361 tttcctccta gtagatctta tcccctcatt ttggcgggat tctttttgtt ctttccagcc
    12421 cggaaccact gccgtttttc cagcatttat gcgggtttgg cgcgttccag taccatcata
    12481 taacagtata tgcatttttt caagcgttct ttcatgctgt ttttctgcgg tccgctttta
    12541 catcagatcg atcgaattca tcgtaattgc ctggcgcagc gcgtcgattt cctcggcgct
    12601 cagcatgata taggactcgc cttttacgat ctctttcaga tataaaaagc agttgtcacg
    12661 gctgtggatt gcattcagga taaaacctgt gttttctttg tcgagcattg ccagcgcaaa
    12721 actcatcttt ccgcccacat cgtcaaacgc atcatatttt acgattccgt atttgttcag
    12781 cgtcatgccc tggatctttt tcagatccgc aagattcttt ttcgtcagct cattctgctt
    12841 gttcagtttg tcaatttcct tgaaccgctg atgaaacagc ttttcgagtg actgggcgtc
    12901 ttttcccttc ataaagcttc tgtacttctg gttcagtctg ctcagacgca gcgacatgct
    12961 gaccgtcata atcatcatca cgataatcag tacaatcaga agaagtacaa caacgccaag
    13021 gtcaattcct accttactaa ataaatcgct cattattcta cctcttactg tattgattca
    13081 aacagatcca tgattctctc gagctcttcc tgggaataat actcgatctc gatctttcct
    13141 ttgtgctttg ccccgcggtg aatggaaacc ttcgtaccaa tgatgctctt gattttctct
    13201 tccagacctt cgtagatggc ttcctcttcc aaattgactt tcttttcttt cttcaccttc
    13261 ggattcagga tctctttgac caggcgctcg gtctcacgga cactgagctt ttcatcgaag
    13321 attttcattg ccgtattgta ctggagctct ttattctcca gcgccagcag cgtacgcaca
    13381 tgaccggttg tcagcatttc atcgacaacc atctgctgta ccctgtcgtc cagtttcagg
    13441 agacgcatcg agtttgtaac cgccgttctg ctcttggaaa cgcgctctgc gatttcatcc
    13501 tggcggagat ggaactcttc catcaggcgt ttgtatgcgt gtgcctcctc aatcgggttc
    13561 agggattctc tctgaatatt ttcaatcagg gaaatctcga caacttcctg ctcggtaaaa
    13621 tctttgatga tgaccggtac ttctttgatt ccggcaagct ttgcagccct ccatctccgt
    13681 tcccctgcga tgatctcata ataatcatct tttttctgca ccagaatcgg ctgtagaact
    13741 ccgaactgtt tgatggattc cgaaagttcg atcaggccgt cctcgtcaaa ggattttcgc
    13801 ggctgttcgc ggttcggttc aatttcggag attttcagtt tcatctcgac tggtttttcg
    13861 acaacttttt caacaacctt ctcgactact ttgacctccg gttttgccgg tttctctttt
    13921 gcttctgctt tggccgaatg tcccggtgaa atcagggaat ccaggccctt gcctaatcct
    13981 cctcttctcg ctgccattac tcttcatccc ccctgtgaat cacttcgtct gccaggagac
    14041 ggtagctctc tgcaccggtg gaccgcttgt cgtaatagtt gatcggcata ccgtagctcg
    14101 gtgcctccgc aaggcggaca tttctcggaa taatggtttt gtagatattc tgatccaggc
    14161 tgtcttttac gttttctact acctgcagag aaagattcgt tctcgcatcg tacatggtaa
    14221 agacaacgcc ttccatctcc agttccggat tcagacgctc ctgcaccagc tcaatcgtgt
    14281 gcataagctg ggataagccc tccagtgcgt aatattcaca ctgaatcggc accagaacgg
    14341 tgtccgcggt ggtcatcgcg ttgattgtca gcatatttaa ggatggcgga cagtcaataa
    14401 taatgaagtc gtactgatcg cggacctgat caattgcgtt tttcaggatg aattctttgt
    14461 cttccacccc gatcagctcg atctccgctc cggccagatt gacattggaa gggataaggg
    14521 aaagattgtc aaaattcaga ggaatgatgc agtcctccag ttttgactct ccaagaagca
    14581 gctcatagat ggtattttcc acttcatttt tctctactcc cattccgctg gtggtatttc
    14641 cctgcgggtc catatcaatg gtcagaactt tttgtcccat ttctcctaaa caggaagaaa
    14701 ggttaattgc ggtggtagat ttacctacgc cgcctttctg gtttgcaatt gcaattgttc
    14761 ttcccaaaat atgtattgcc tcctttcatc tttttgtttt ccatttgcag tttttccttt
    14821 ttacaggaaa tgacctgccg gcacttcttt caaaaaaatg attcatgcgg caggtgtttt
    14881 ggcatatata taggaaaaat ctccttttct atgccagtct atctgaggaa ttcttcagat
    14941 tctatctcaa aattgcccca atttgggaca aagatatttt atcatactac gccggatttt
    15001 actactggaa atgtttcacg tgaaacattt tctgcatttt tgcatgatta ggcggtttga
    15061 aacataagaa aattggagat ttttggcgat gtttcacgtg aaacattttg aaaagcactg
    15121 ttttacaatg atttttctcg gtttttacgc tgtttaaagg ctttttggaa ttttttcagc
    15181 gatgattttg cattttttga tggtttccaa gtaattctgc aatgtcagaa catttttttc
    15241 tgtttcacac agtttttata aaatgtttca cgtgaaacat tttaattttt gacgtttttg
    15301 aggtataaat atactggaaa cgttatttta atcgtgtgat gctctttttc aaggaaatct
    15361 caggtgatta ggaagaaaaa atcaacttat caggatatta ttgatacata gatcaggaat
    15421 caaaactgtt tatggttgtt cggtgatcgt aaatgtctct tttgtatata aaatgtattt
    15481 ttattcacca gggctctgtt ttttcatcgt tcttcctcgc ttttttcaaa ttttcagcat
    15541 ttttctcttt attttcatca gtaagaggca taatccgtga gacatcctct ttttcatttt
    15601 ttctccagtt tttctctttt tgggcggttt ttctttgatt tttttatttt acaggccaag
    15661 ctgcacattt tctgttttta tcgttttatt tgatttcact ataatttttc tgtttttatg
    15721 gttttaatat ttttctctcg ctgccacgtc ttttatcctc ttttccatct ttctggctta
    15781 cctttctctt tttttgtaaa caatctcact ttttctcttt tatcaacatt ttttattcct
    15841 tctctccgtt ttttatccct catcgccatt tcacaccatt ttcttgcctt tcactgcctc
    15901 tccctcccgt ttttattctt ttcgcccatg tctatactac attttcccct tttcagtcgt
    15961 tttttgcctt ttctcaactc atttttatct ttttttgctt tttccgcctc ttccatcgtt
    16021 ttacaccctc tttttgccat tttttttaga tcgtccttca ttttttcgtt tctacttatg
    16081 tcactttccc cgtctcattt ttcacgtttc acgtctctca aaatgtttca cgtgaaacat
    16141 cgttttttac gattttcggt cttcggcgat caatgagaag cgatttgctt cccgactttc
    16201 cgaaaatcac tctcattttt cacctctctt atttttaaaa caatcgtatt ttctattcta
    16261 tttttgattt tcccgtcttt tctacatttt tcctgtattt ttgcgtgttt cacgcagttt
    16321 ttcgtctctg ctttctctct tttattaaat tatttaatta tatttttata ttttcgtatg
    16381 aattgaactt ttacattcgt tttttcggat tttccaaacc atttactaaa cgcattttga
    16441 ggccattttt tcagggattt ttgccaatct gaaaggattt tatcccatag tgccgttttc
    16501 atgcttttat gtgtattatt gcatcatttt ttctctgttt tttacagtta tgtaaaccat
    16561 ttaaatatgc cattcgccag tagttgacat aatgtagttt tcctagaatt gtctacgctt
    16621 ttcgccgaat tgcgtcttat tttcgtctct ttttctcatt tttcccttaa tttttgcatt
    16681 gtatctttcc cttttttcgc ttataataag ctcaacacaa cgtttcgaac cgattcctcc
    16741 cctccgggag acccctgaaa ggagttttat catatgaaac acagcaaaaa actggtcacc
    16801 ttgagtgtcc tcaccacgat gtccggcgca gcgatttact ttctgaataa aacgctcgat
    16861 accgccgccg tacggaaaaa cctgctcgcc tcggctgaaa aagagatctt ttcctggcag
    16921 ttcggcgata ttttttacac aaaaaaaggc accggaaccc cgatgctctt acttcatgac
    16981 ttacattgtg cctccagcgg ccgcgaatgg cagtatattg aggatgctct cgcccaggat
    17041 cacaccgtct acacactcga tctcctcggc tgcggccgtt ccgacaagcc ggcgatcacg
    17101 tacacgaact ttttatacgt acagctcatc gtcaccttca tcaaacaggt catcggatgt
    17161 ccaaccgacg taattgcatc cggtttatcc ggctctttcg tcgtaaccgc ctgcaataca
    17221 gccccgaaat gcttcggccg aatcatgatg atcaacccga ccgaccttgc caagctcaac
    17281 aaaatcccgg ataaacgcag ccgcttcctg aaacgcatga tcgagctccc gctcgtcgga
    17341 acgctgctgt accacacgct caccggccgc tccaacatcg agcttttatt caccgagagc
    17401 tactaccaca acccgttcca ccgctcgccg gaggacgtag acgcattcta tgagtcagcc
    17461 cacctccaga gcagcgccgg aaagtacctt tttgcaagcc ttgcgggcaa ttacgtgaac
    17521 ctcaacatcg gccacgcctt aaagcacatt gacaacagca tcttcctcat cggcggcgag
    17581 gaagagccgg gcatcgcaga aacctttgcg ctgtacacag cgctcaacac ctccatcgaa
    17641 acccagatcc tgccgaaaac aaagcacatg ccgcagatgg aggccccgca ggccctctta
    17701 gaccagatcc agatcttctt cgcaccggta cagtaatacc cccttctgct cccggcctcg
    17761 agcaaaaaaa tgagacaaaa aagcggagga tttactgata tttccatgtc agtaaatcct
    17821 ccgtttttcc atttttaacc cggctcacag tccatttcca acgcatcttc ctctgcgccc
    17881 ggataatccg ttggcggcgc tgtatgcgtt tccattccct cttttatttc atccatcgta
    17941 taaatattaa ttctctcctc cggcgctgca tccgggtcaa attccaccgt ttctcccagt
    18001 gcaagcatca ttggctcatc cgcatttctt ttctcatctg caatctcata cgcgtttaca
    18061 tattcacccg acgcagtctc tacatactgt ccaaacccgt cgcaaagccg gtaataaagc
    18121 tccttttccg catctttctc cagaaaatac aaggatttct gcccgatcac cggatcaagg
    18181 tcgcagaagc tgatctgtat atagttctcc cgccgttcct cctccgaata ctccgctttc
    18241 agctcctctg cgttccagta cgtaattccc gcatcctcca tggactgcag atactcttcc
    18301 accgtaactg ctccgcccgg ctttcgaacc gtaatatgtt ctccggcttc catatcacct
    18361 ttcaaaacgt catccacccg cagtgaaata ttggttgcaa tctcaattcc cccggcaaaa
    18421 cagctataat cctctaccgt tcccagaaca atgagatcgc ttccctcttt ctgctcataa
    18481 aagctattat aaaaccgatc ccactccttc aaccctccgt actgcatatt tacctttgcc
    18541 ggatccagat tttcaaaccg cattttctcc tctgtttccg ccgtttctgc attaccgcag
    18601 cccaccagca gaatacacaa tactgtcaaa caaacctttt tcatcctttc accccccatc
    18661 caccgtcttt gttatactcc cattatactt cttccgcttt cattttccca taaaaaagcg
    18721 gaagacttac tgattttttc actctcagta aatcctccgt tttcaatttt aaccagattt
    18781 taatgactct ttattccgct gattctgacg tgctgtctga agaactgtca tcttccgtct
    18841 ttgccgcaag cagtttcccg taattttcca ccatggtctt cacgttattt ttattcacaa
    18901 gagacggaac tccgtcctca tctaccgtgt aatagaagct cgaatcatac gggctgtaag
    18961 caaatgtcag gtcgccgtct gtcatatgga agactgcggt aaactcggca tccttggcct
    19021 gcacgaggct ttcatccttc gtctgcgcct tcaggccaat gagcgaagaa tagaacgtgg
    19081 taaacgttga tttatcaatc gtttcatcgc cgcagatgta actgatttcc tgtgttgtgg
    19141 aatcgctgtc gtcatccttt ttctcttcca tatggcgtac atacgtcttc gtctcgccgt
    19201 tatacgtaat atcaagactc gtcagatccg atacattttc catgccgata tacatgttcc
    19261 agaaatcaga tgccttcttt ccggtgaagg tttcaacatt cgaagccgtc atcagatgaa
    19321 cttcattcga gccgtccagc ttcacgtaat aagaaccgcc gtcctcattt gcattgccga
    19381 catacaatac cagatctttc tccaccgtaa cggttgtcgt ttcggaagaa gagctcgttg
    19441 cagtgctgtc gcttgatgaa gaatctgctg tggaatccga agaagtatct gccgttgttg
    19501 tatcgtcagc ctcaacctcc tgctcttctg tgtaatgcac gcggatcgtc gtctttggct
    19561 gatccagtcc gtactgggaa agatccgatg cgttgtacgt aacaagatct ccaaagctaa
    19621 gagatgccac cgcacttgtc atggtgccga tcgaatccgc atcaccgcca tgtgccgctc
    19681 catctgcgtc cacaacctgc cattcatccg tctggagtgc cgaatcagca gcgcttactt
    19741 cttttgtcag cgtatatccg ttctcaccct ccacctgcag ctttgtaatg ttttccgccg
    19801 tcacagatgg catggattcc gttgtcgccc actccatcat atcggacggg aacatcgttc
    19861 caagcgatgt gcttaccaga taaaccttac tcgtatcatc gttcagataa atatagtatc
    19921 tgctcgagct gttcttatct ccgacatgaa tcgtcgtttt ctcgtctccc gcagtcacta
    19981 caacctcctg agacggcgtc tcgagcccaa aatcggccat agaatcgatt tcatcgcctt
    20041 caatgactct ttctgctgtc atgctgctaa gagagcttaa aacgctcgtt atcttgccct
    20101 catccagggg gaatccatcc tcctggtcgt atgtccagat atcggtatca tcgtctcctg
    20161 ctgttttcgt aaaagacacc gtctctccgt tgatctggaa cgaaagcgct gttacatcat
    20221 cttttgaaaa atcaaccact ttttcggcgt ctgcaccaga atccgaactg ctgctgtccg
    20281 catcggcggt gctgtttttc ttcgtcagca ccaccagtac ggcaagaagc agtaccaaaa
    20341 ggatcaggca ggccgccatc tttccttttt tcttcatcac tgttttctcc tcttcatcca
    20401 gatcatcacg ccaatcacaa caagtaccag cggaattccc gcaatggtaa taatagacca
    20461 ggtgttaact gctccctgtg taaataccag gtttgtatag cttgtgctct tgcttggaat
    20521 aacaacggtc tgctccacat cacacatgga ggtcagtgca gtcgctgcca gctttgagtt
    20581 cgcaccgctg attgcctggt caatctggct gctcagcatc gatgcactcg agaaataaat
    20641 gatctgtgtc tctttatcat ctacggtctc tgtgatggaa acaccaaggt caaatgcgcc
    20701 cgtctcactg tccgcactct tcgaccaggt gctgttctcc ggatcatttt caatataagc
    20761 gtcctcggtg gtcgtcagaa ggctcttgat cgtaatggta tcacgataag aatcgagctt
    20821 ctgaattgcc tgtgcaaccg gcatcagaat gtaattactc tttacttcgg ttgtaatgtc
    20881 atcactctga atgttcggca gcagataata cggcatctgc ggataataat gctgggaatc
    20941 ggtctccaca acaattccgc tggagcgttt cacgccgtag ttctccagta tggaatccag
    21001 attcggcata tccgttccgg tatagctggt gaacatcaga agttttccgc cgttttccag
    21061 gtaggtaatg actttatttg cctcatcctc ggaaaaatcg gtggtcgggg acatgatcac
    21121 aagctcctcg gcatcctccg ggatatcgtc tgtcatcagg gcaagatcac tgatttccac
    21181 attgtttttg ttaatggcat cactgaagtt tgtagagaga tcactctcgg aagcctcgcc
    21241 gtggccggtt acccagtaaa ccttcgggtt gttttcactc gtcacatacg tgatggcgct
    21301 ggtaatctga ccctcgccgt caaagccggt tgtctgtgtc tgataggtct gatagtttgt
    21361 ggaagtctcc cacatatcac tgttgctgac caccttgctc ttatcgccgc atacgacaat
    21421 tacactgttt gcagctaccg tctgatcggt atactgactc gtaaatttcg gatacagatc
    21481 cgggtctttt gtctctactt tgatatggtc agaaagctca tcgtagtgct ccagcagctt
    21541 ctcgatggtg tcatcctcac tgcccttctg cacaatataa tacagggtaa cgtcttgatc
    21601 gagatccttt aatgccgttt tcgtctcatc tgtaatggaa tacattttct gcgtactcag
    21661 gtcaatctgt gttttactgg tcggaatctg cgccacaatc aggttaataa ccacaatcac
    21721 agccagaaca atcaccgtca taatcgaaga ataagagccc tgtttcaggt gctttttctg
    21781 ctcctcgccg gtaaacactt ccttcagatg ttctttggag aacgtctttt tcagcttttc
    21841 tttgatattg aaattcattt tatgctgttt cttttctttc ttttcgctgc tcatcgtctc
    21901 cacctcctaa ttccagcgtt tcttctgcag ggactgcatt gcaaggaaag tgcagattgc
    21961 aatgacggac aggtagtaaa caacaccgcc aatgtccaga ataccgttca caaagttatc
    22021 catatggcct gccatgttga atacaccgag cagatcctgg attttgccct cgagaagggt
    22081 ggattttgtg aagtaaactg ccgtcagcgc accctcgccg atcacggcaa tcacaccggt
    22141 aatgatcggg tttttaatca tactgccgat ccagaggcat accagcgcga tgatgataaa
    22201 gaaagcaaag aaagagcttc cggctccttc cgggaagaag tttgcgatac cgctctccac
    22261 gtagcacagg aacaggatca caaagccgac aacggctgcg atgacctggc tctcggtgat
    22321 ggaggagacg aacacgccga ccgaaatata ggctgccccc agcaggaaat aaccaaggat
    22381 cgaagtgtag gatacggcgt aggaaaccgt tccgtgggtt ccaagaagca gcggatacag
    22441 gcacataatc agcactggaa tcacataaat ggtgagaagc gccagatatt ttccaaggat
    22501 aatatcggaa atttttaccg gtgcggtaag gagcagctga tccgtcttat ttttctgctc
    22561 ctccgacagg acacgcattg tcagaaccgg tactgcgatc agcagaataa atgcggaatt
    22621 ctgcagcgcg tatccgatct ccgggtaacc ctgctgcagg ttgatggccg taaaatacag
    22681 gccgaacagc gccagagtaa acgccataaa cagatatccg atcatgcttg tcagatagct
    22741 ttttaactct tttttataaa tcgccgtcat tgtgcgtctc cctcctcctg ttttgtttca
    22801 gcggctgatt ccgctgcatc tgtgcttttt tcttcagacg tttccccgac agccgttttg
    22861 tctgcttctg gttcacattc tttttcacat tctttttgtt tcagctgttc tttctctgct
    22921 tttttcagct ttcttgcagc ctttccggaa tcatcggtca gctccaggaa gacgtcttcc
    22981 agagacatcg aggaggaagt cagctcgagg atcgggcagt tggcctttgc cagtgcatag
    23041 aacagctcct cgcggatatc cttatccttt tccgtggtaa tttttacctt aatctgctcc
    23101 tcattcggcg tattttcata ttggatctct tttgcatcga gcgcattaag cgccggaacc
    23161 agctgctttc tcgttccgtg aacggtacat gtaaccgtgt ttgatccaag catcagttta
    23221 cttaagtttt ccggggtatc actcgcaacg agctgtccct ttgcgataat catgacgtaa
    23281 tcgcagacgg cgctgacctc ggaaaggata tgggaactta agataacggt atgttttttg
    23341 cccagtgatt taatcagatc gcggatctca atgatctgct tcgggtccag acctacggtc
    23401 ggctcgtcga gaatgatgat cggcggataa ccgagcaccg cctgtgccag tccgactctc
    23461 tgacggtatc ctttcgacag gttcttgatc agacggttct gcatatcggt gatcttcacc
    23521 agattcatga cctcaagaat ctgtttcttt ctctgatcct ttggaatttt ctttaattcc
    23581 gccacgaatt tcagatattc cagaacggtc atgtccgtgt agaccggcgg cagctccggc
    23641 agatagccga tgcatttctt tgcctcctcc ggctcctcca ggatattgtg gccgttgatc
    23701 agcacttcgc cctccgtaga tgccagatag ccggtgatga tgttcatggt cgttgatttt
    23761 ccggcaccgt ttgggccaag gaaaccatag atctggcctt cttctacggt aaaactcagg
    23821 ttgttgacag ctacgtggtc gccatagcgt ttcaccaggt ttttaacctc tatcaatttt
    23881 ccttcctcct tgtttcttaa agcagtccca gcccgccctt gggggtgaaa gagccggaga
    23941 tgctctcatt ttctaaagac actttatctt attctgaaaa tgtgataatt tgctggaaaa
    24001 tttgtgaaaa aacggtgaag ccatggagca gtttgtttgt ttttcgacga aaaatcaatt
    24061 tcttttacca taagaatcat gtatactata acgtatccgc ggacggacgc ttttccattt
    24121 ttgttccgcc gcgaaacatt acgccggact ttttcccgtc cgggactgga gattttagga
    24181 gactctatga ataaaaaaga ctactactgg gccaagtata tgctgatttt cggctttttc
    24241 tgcatctgct tcggcacttc cattttcctg aaaaccgaac acgcccccga cgaggcgatg
    24301 cggatgctga ttccggaata catcgtctca caccacacac tgccgaacgg catggaagaa
    24361 tcggtgcgcc atccgctgtg gggattctcc tatgcgctgt atccgtatct gaccgcgatc
    24421 atcagctccg tgtttatggc cattacatcg ctgtttacga aaagtgcagc ggctctttta
    24481 actgccgccc gcctcaccag cgtcctttcc ggcaccggca cactgatcgt tgtgtttctg
    24541 atcggcgaag agctctttga acggcgcgaa tccgcactgc tcggcggtat tttcgtcggt
    24601 tttctgccgc agtttgtgtt cctctcctgc tatgtgaaca acgactcgtt cgccgttttc
    24661 acggtcgcac tgatcatcta tttctggatt cgcggcatga aaagcgcatt ctgcaaaaaa
    24721 gactgtatcg gcctgggagc aggctgcgga ctctgcgcgc tgtcgtacta taacgcctac
    24781 gcgtacctgc tgtgcagcat tctgctgttc ttcgcgctga tgattcattt ccggaaaccg
    24841 gcaaaagaaa tttttgtcaa agcgctggct gttttcgcta tcgccttcct gatcggcggc
    24901 tggttcttca tccgcaatgc cgtcatccac gacggcgatc tgctcggcat gcgcacctcg
    24961 aatgaatccg catcgctgta cgcagccgat gaatacaaac cagagaatcg tcagaccccg
    25021 gcatccgaag gctggagctt ccagcagacg tatttacaga ccccggaggg ccgcacctcc
    25081 aactggctgt tctcgaccgt cagcagcttc atcggctcgt tctcctacat gaccgtccat
    25141 ctcccgatgg tgctttatct gctgtacggc gccctgatgg cattcggatt cctagtgttt
    25201 ttgttcctcg gcatggttcc gcactggttc cgcaaaaagc cgcagcttct cctctttgtg
    25261 atgctgttcc tcgcctgcct ggtcacgctg ttcatggaca tgtacaacac gtatttcagc
    25321 gattaccagt ctcagggccg ctacctgatg ccggcgctcg tccctctgat gatctggatc
    25381 gacgacggct actcttctct caccgcgaaa cttcccgccg agtggaagcg cgcctcctgc
    25441 catctgacac tcctgcctgg aacgatctgg ctggtactgt tcctcatcag ctacatcgga
    25501 tttctgattc cgggctgctg tatggcatcc taaacgtttt cagaacgtca aaaagcgctg
    25561 caaagaactt ttttcttctg ttctttgcag cgcttctttt attttctttg aattttttac
    25621 tgaatcggct ccttcgccgg tgttcccgct tttctcgggt actttttcgg cgttgccttc
    25681 cccttctcga tacagacaaa cgatctctca tcctccgttc ccgtaagctg gaagtaaacc
    25741 ggctcttttg cctcgccgcc gagtacatgg atcgcttttc cggctgcggt aagctcctcg
    25801 cttaatttgc ccgatttata cgaaacaaac cgtccgccca ccttcacata cggcagacaa
    25861 tactccgaaa gcgaactcaa attcgccacc gcgcgcgata ccacgagatc aaactgctcc
    25921 cggtactcct tctggcgtgc gaaatcctcg gctcttccat gaatggccgt aattttggaa
    25981 agtccaagct gtccgatcac ttcattcagg aatttcactc tcttattcag tgaatccagc
    26041 agtacaactt ccacctccgg aaacgcgatt ttcagcggga ttcccggaaa accggctccc
    26101 gttcccacgt cgagaatgcg tcccgcctgt gccacatcac agaccttcac cagcgacaga
    26161 ctgtcgagaa aatgcttcgt aatcacctga tcgaactccg taatcgccgt cagattcata
    26221 aacgaattcc actccaccag cagctcgtaa tactgcagaa actgcgtctt ctgctcctcg
    26281 gaaagtgtga ttccaagctc aaaaaggccg cgctcaaatc cttcaagatt gtattctttc
    26341 atgttgttct ccgttctact ctattcttcc aagtgtccgc tgcacttttt gttacgattc
    26401 acacagcgtg aaagcacttg ctgcttcgct gcataagaac gtaattcagg catgcatatc
    26461 tgtcatttat ttatgtccca gatacaccag cagaaccgaa atatccgccg gggaaacgcc
    26521 cgaaatacgt gacgcctggc cgatgttgat cgggcggtac aggttcagct tctgtttcgc
    26581 ctcgatgcgc aggctctgga tctcgtcgta attgatgttc tccgggattt tcttctcttc
    26641 catcttcttg aactgctcca cctgcttcat ctggcgcttg atgtagccgt catacttgat
    26701 gttgatgttg acctgctcct gcacatcctc cggcagcttg ggtcttttcg gatctacttc
    26761 tgccagcatt ttatagttca gctccggccg gcggatcagc tctgcaagtg agcttccgcc
    26821 ggaaagcagc gtcgagccat attctgccag cagttcattg acattcgagc tcgttcccac
    26881 atacgtattc tccacgcgct caatctcttc ctggatctgc tgttctttcc gtttcagcgc
    26941 cgcatactgc tcctcgctga tcagtcccac gcggtatccg tatttgcgca gacgcaggtc
    27001 cgcattgtcc tggcggagca gcagacg
//
LOCUS       DAOMUF010000009        25115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_283_length_25115_cov_3.761093, whole genome shotgun sequence.
ACCESSION   DAOMUF010000009 DAOMUF010000000
VERSION     DAOMUF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 25115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25115
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_283_length_25115_cov_3.761093"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="PLN77_01440"
     CDS             complement(<1..177)
                     /locus_tag="PLN77_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HRM42977.1"
                     /translation="MEVITYKCPNCGGDLTFDPASGKYKCEYCLSSFTQEEAEKANPK
                     AAEALNGEDDAAGAQ"
     gene            complement(234..1487)
                     /locus_tag="PLN77_01445"
     CDS             complement(234..1487)
                     /locus_tag="PLN77_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRM42978.1"
                     /translation="MGIIRAAVNAVHGSLADQWLETVEPYEMGEHTVFTEGILVRKGQ
                     NKKGSQTISNGSVIHVYDNQFMMLVDGGKIIDYTAEPGYFTVDQSSSPSMFSGSLDAA
                     VKDTFERLKFGGQTPHEQRVFYINLQEIKGIKFGTRNPVNYFDQFYNAELFLRAHGSY
                     SIRIVDPLRFYAEAVPRNASRVEIEDINEQYMNEFLEGLQSSINQMAADGIRISFAAS
                     KSAELSRYMADAMDESWRAMRGMEIQSVAIASLSYDEASQKLIQMRNEGAMMSDPSIR
                     EGYVQGAMARSMEKAAANPNGAMNGFMGVQMGMNAFGSSFASASASNQQQMQQQAAAK
                     AQQEAAKGVWKCSCGTENTGNFCSNCGSAKPMVWICGKCGTENSGKFCSNCGSPKPEA
                     AGKWVCACGTENTGKFCSNCGKPRQ"
     gene            complement(1599..2706)
                     /gene="prfB"
                     /locus_tag="PLN77_01450"
     CDS             complement(join(1599..2654,2656..2706))
                     /gene="prfB"
                     /locus_tag="PLN77_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156797306.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRM42979.1"
                     /translation="MELGTYDKPLVEVRDSLDLANKEQRIEELERKMEEPNFWDNPEE
                     AQEQMKQLSSMKEDRDTYQKLVSAKEDMETLIEMGQEEDDDSVVPEIAEMMEEFKASF
                     DAIRIKTLLSGEFDKDNAIIKLNAGAGGTEACDWCGMLYRMYTRWAERKGFTIEELDF
                     LDGDEAGVKSVTFQVNGENAYGYLKSEKGVHRLVRISPFNAAGKRQTSFVSCDVMPDI
                     DKEIDVEVRDEDIRIDTYRSSGAGGQHINKTSSAIRITHMPTGIVVTCQNERSQFQNK
                     DKAMQMLKAKLYMLKLQEQADKLSDIRGEVTEIGWGNQIRSYVMQPYTMVKDHRTNAE
                     TGNVDAVMDGGIDLFINAYLKWIALGTGAKEEEA"
     gene            complement(2848..5418)
                     /gene="secA"
                     /locus_tag="PLN77_01455"
     CDS             complement(2848..5418)
                     /gene="secA"
                     /locus_tag="PLN77_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRM42980.1"
                     /translation="MKFTEKLFGTHSERELKRIYPIVDKIEALRPTMQALTDEELRAK
                     TKEYKDRYNGGESLDSILPEAFATVREAAKRSLGMEHHRVQLIGGIILHQGRIAEMKT
                     GEGKTLVSTLPAYLNALTGRGVHIVTVNDYLANRDAEWMGKVHRFLGLTVGVVLNDMK
                     NDERRQQYACDITYITNNELGFDYLRDNMVIYKEQLVQRELAYCIIDEVDSVLIDEAR
                     TPLIISGQSSKSTKLYETADILAHQMQRGEASGEMTKMTAIMGEEIEETGDFIVNEKD
                     KFVTLTDDGVKKVENFFHIENLSDPENLEIQHNVILALRANYLMHRDKDYVVKDDEVL
                     IVDEFTGRIMPGRRYSDGLHQAIEAKEHVKVRRESKTLATITFQNFFNKYEKKAGMTG
                     TALTEEQEFRDIYGMDVIEIPTNRPVIRVDMDDAVYMTKKEKFHAVVEEVKEAHAVNQ
                     PVLVGTITIETSELISKMLRREGIPHQVLNAKFHELEAEIVAHAGEAGAVTIATNMAG
                     RGTDIKLDDVAKAAGGLKIIGTERHESRRIDNQLRGRSGRQGDPGESRFYISLEDDLM
                     RLFGSEKLMGIFKSLGVPENEQIEHKMLSSAIEKAQKKIEGNNYGIRKNLLDYDQVNN
                     EQREIIYKERRRVLDGESMRDTIFKMIADTIDNTVDLCIGENTEQSEWDLTELNEMLL
                     SIMPVKAVTKERVEKISKNELKQQLKEEAVKLYEEKEAEFPEEEQIRELERVVLLKVI
                     DQKWMDHIDDMDQLREGIGLQAYGQRDPKVEYKMAAYDMFNEMLESIQQDTVRLLFHV
                     RVEQKVEREQVAKVTGTNKDESGPRKPVQRAERKIYPNDPCPCGSGKKYKQCCGRKPV
                     "
     gene            complement(5653..6180)
                     /gene="raiA"
                     /locus_tag="PLN77_01460"
     CDS             complement(5653..6180)
                     /gene="raiA"
                     /locus_tag="PLN77_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRM42981.1"
                     /translation="MKITISGKNIDVTEGLKAAVNEKLGKLEKYFTPDTNVIVTLSVE
                     KERQKIEVTIPVKGNIIRSEQVSNDMYVSIDLVEEVIERQLRKYKNKIADKQQEAGNF
                     QKEYLEHDYLEDEDIQIIRTKKFGMKPMYPEDACVQMELLGHTFFVFRNAETDEVNVV
                     YKRKNNTYGLIEPEC"
     gene            complement(6481..7257)
                     /gene="hisA"
                     /locus_tag="PLN77_01465"
     CDS             complement(6481..7257)
                     /gene="hisA"
                     /locus_tag="PLN77_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HRM42982.1"
                     /translation="MKFRPCIDIHNGKVKQIVGGSLKDQGNQANENFVSEQDASYYAQ
                     LYEEKGITGGHVILLNKKDSEYYPETKRQALLALSTYPGGLQVGGGIDAENACEFLTA
                     GASHVIVTSYVFRDGHVFYENLEKLVTTVGKQRLTMDLSCRKRNGKYYIVTDRWQKFT
                     EEEVTEPLLHHLTEYCDEFLIHAVDVEGKASGIETELVELLAKMKDFPVTYAGGVGNF
                     ADLELLKKVGNDHVDVTIGSALDLFGGNMKFEDVLAVCRR"
     gene            complement(7583..10075)
                     /locus_tag="PLN77_01470"
     CDS             complement(7583..10075)
                     /locus_tag="PLN77_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRM42983.1"
                     /translation="MKKEIKCPRCRRRIQKEDAYCQYCGCHVAEEKKKKRKKKIRRFL
                     VLVIVIVLFLGILLAIAVGSQKKQIQGWKERQLEEWKQKQEKVQADEGDQSQKQNKNQ
                     NGEQSQDSQDVKEPGTMKKCANGDTVYQPSVSTVSYDEDTGLLYYENLLIVYLIENSS
                     QEEREALAAKVDGTLVGNMSGSMNVIEILVEDTAYADLEQKAEILMEEENVLYSSTEI
                     PMMMENTADENPWEAGEQDKKNNDHNESKPDGNDWWAEAIGAFSAWDYVDSHEQDLAN
                     VAVGIMDDGVDAEHEEFQKNGESKIHWLEDYKENLAFDHGTHVTGLIGAENNQAGIRG
                     VADRTDLYFVSWWKEDLFSTGEYVNVTKQMIETMLSEHTACVVNNSWATVIQSREQYL
                     KDTLIGERPVWEEKIAEFLIEKGKIDQKYELYESYQKELETSAKYTSSQMIVMLFELL
                     LNQQERFLIVQSAGNGYDNGGVGFDVQKTGDYCGINEEVYNRLDGETHRLSRSGYSYE
                     KIRNHILIVGAVQETEKGYQMTEFSNYGSNIDIVAPGYDVYSTITEKDDSYTEENPGH
                     ENLRTVKNGIKYGNLPGTSMAAPLVSGSAAVLWSVAPELSAEEVKETLISTAGTARST
                     CQEDKREEYPMLNLKAALEKVAKKDATHVILETFYNNGEKTHDLFASEENRDQEYAVI
                     TGLDQDENVVWPIETEKSPAAEITANTEIGIYEDRYYYAHCGVIYAVRLRDGKEIWHS
                     ASSHGSMTGTDFGPDGTLYYCSFYGPDFGAIDKDGNELYEVESFYPGYYWAYEVHYEG
                     DHVDVKMDGTPSGEETVIRVNLSDYSYSVVQE"
     gene            complement(10088..10777)
                     /locus_tag="PLN77_01475"
     CDS             complement(10088..10777)
                     /locus_tag="PLN77_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM42984.1"
                     /translation="MKTKAKYVIVILFLCTIPLYFCRMIGWKDDAIRLYDVIIYGMES
                     QEGDSFVGGMIWKLMQESVFENINAWLIGTAAVTLVGSVLVTLLNGKKTYVLAIVLSI
                     VTNGGLAWSMQRLSWGWKNKVDYNGQMTGMPLRMEFVWVWIALHVLILIFALWGMVSK
                     EKSASKKSVSRKIEPEKIKSKKTESKKIKPEETVSKNPAASKMRSACPSCGRIPKEGE
                     KFCTRCGSKLQ"
     gene            complement(10806..12074)
                     /locus_tag="PLN77_01480"
     CDS             complement(10806..12074)
                     /locus_tag="PLN77_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42985.1"
                     /translation="MENMKFSDAREFQDGLAFVRIHGKGESYKGFLDKKGKLKFYIPF
                     DEDAIDADIYRYDVNFNNGYNWFVYDNIFYVIDTDGMIKSQYDADKVADYGGGYTWLE
                     EEENVSWDDAGFWKYTLYSPEGGEVCDYTVSNQDLENLSFKRSYMGDGKFLYKKMDGT
                     KEVCVLVEPELGKETELNVSFDKAEEHGMRDGLIIETGAMDDGYWAGDENPFHITVIG
                     NGEEKEIEIPSQYLGPFGGYPTLLDWTKKYALFTVRQDDQDIYFLCDLETGDIKKYTG
                     KYAPYFKYYSTTTSCIEDNVLALSMYGEDNQFYVCLINADTMKEIADPIAGESFSMED
                     KTLLIDQKELYDLSGNLLYTVEDGKKGELVSDGILQVTYSEEEKETVDGESEYVEVDK
                     TDYYDLKGKKLFSEMDTADSKMVLEPSEEV"
     gene            complement(12172..12666)
                     /locus_tag="PLN77_01485"
     CDS             complement(12172..12666)
                     /locus_tag="PLN77_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42986.1"
                     /translation="MKKAGSRILIITFGIMAAIAILYPWKFVPEYGILQMTESGEKAG
                     KTAESQESDEEYMQVADRAVEALFTGDTDTLLSLLPERYFGQYGETLQWIIENAVEEE
                     LQKLQDEVGDIQITHWPVRSEKQSEGNMEKIKSHYGEEGDTVTDARNVFVEVSASGIE
                     PEEL"
     gene            complement(12807..>13324)
                     /locus_tag="PLN77_01490"
     CDS             complement(12807..>13324)
                     /locus_tag="PLN77_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42987.1"
                     /translation="ANETDLFLESGSDFWHAAIKNDEVSDLEEMPEGYVKSVTPTEIY
                     YTSNFYTEGDNAYYDVNVIENGKSRCLAKEVLEDYVKIYEDGTVMAYTDRNSDGEYEL
                     SIFDKKGNKTKIADGVTKAIREEDGDIVYDSRHDLMLYQKEESERIGIGIVDFWYADE
                     MKTEQNFRLDW"
     assembly_gap    13325..13334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13335..14827)
                     /locus_tag="PLN77_01495"
     CDS             complement(<13335..14827)
                     /locus_tag="PLN77_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM42988.1"
                     /translation="MKKCPVCGKEWSDATRFCGSCGAKLEDGNIVVPDIVPDDGKEEQ
                     RKKTEPVKPSVKKTIEPVRPKTRETAENKKIAEEKEEEKNTAERKRAEYNKSDAVLPV
                     KRRKKGWKIATALGLTAIVAVGAVVFCVKSGENMEHPFVELYGDSYEILTKEKLGHTI
                     TITDNGLDWLGDAVMQLSPDRKYLYYMENYDISTGTSRLYRCEYQKLSKKEEKNEKYK
                     TKVAANVRVFYPLENGKLLYIDKDSELFCYDGEVSVKIADSLQGYYQLKDGTGLVYTK
                     GNADEGFDVCGVSYNNPTEEVFLADGMTDVMLADDQEHILCRRYDGQSEDDQLVFLTS
                     FEEETKELGTPVSDMYAWENGAVYYFASGENTECELEDFVIDSRGNTEQWQELQKEAE
                     SYHPVPTECQSLYEYKDGEIKQITANDINNAGLYRGNSVLYLDVKKMEPVDLAEVKDA
                     DYMIDLLDQRIYEQGMYVKPTSRKNSIHIIGEVAKKIEDIDEEGENF"
     gene            complement(14983..15456)
                     /locus_tag="PLN77_01500"
     CDS             complement(14983..15456)
                     /locus_tag="PLN77_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRM42989.1"
                     /translation="MAFLDGVKGKISKAGYSTMQKAKDLSEITKLNMAVSESETKINE
                     IYSEIGYKVYCAYRENPLEEVKEEIGQIRELEEAMEACKLQIQAINAMNSCPRCGAKI
                     KPEMMFCSSCGMKLQSEEQETVEEEQERPAFCSECGAPLEPDMKFCTVCGHKVDE"
     gene            complement(15660..16259)
                     /locus_tag="PLN77_01505"
     CDS             complement(15660..16259)
                     /locus_tag="PLN77_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM42990.1"
                     /translation="MENENIEKQEFFKTYLGIKNKNVLSSLEQMAYVWKVKDKEVIIR
                     EGEPLPCVPFLISGIVKGYCESKGDGKEHVSCFGYLKGDIVVGISNLSVNVKSLLTIE
                     SISSCTMLCVPIPELLKLIEQSPEMMRLYNRALSLAYKKLIEHEDIVANHRAKERYQY
                     FVDTYRDLVDVVSKKDIASYLHLSPESLSRVLKSYKEEK"
     gene            complement(16590..17801)
                     /gene="dnaJ"
                     /locus_tag="PLN77_01510"
     CDS             complement(16590..17801)
                     /gene="dnaJ"
                     /locus_tag="PLN77_01510"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRM42991.1"
                     /translation="MAEKRDYYEVLGVDRGADDATLKKAYRKLAKKYHPDMNPGDKEA
                     EAKFKEATEAYGVLSDPDKRRQYDQFGHAAFENGGGGAGAGGFGGFDFGGGDMGDIFG
                     DIFGDLFGGGGSRRRANNGPMKGANLRAVVHITFEEAVFGCQKELEINLKDTCTTCHG
                     TGAKPGTSPETCSKCNGTGQITYTQQTMFGSVRNVTTCPDCNGTGKIIRDKCTACHGT
                     GYTSSRKRIQVNIPAGIDDGQSIRIREKGEPGVNGGPRGDLLVEVQVTRHPIFQRQDM
                     NIFSTAPITFAQAALGGDVKISTVDGDVLYTVKPGTQTDTKIRLKGKGVPSLRNSNVR
                     GDHYVTLVVQTPTKLNEKAKEALRAFDEACGNRPAAEAKAADGENTAEQPKKKKGFMD
                     KVKEAFEDIDK"
     gene            complement(18027..19904)
                     /gene="dnaK"
                     /locus_tag="PLN77_01515"
     CDS             complement(18027..19904)
                     /gene="dnaK"
                     /locus_tag="PLN77_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRM42992.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGKPTVIANAEGERTTPSIVAFTK
                     TGDRLVGEPAKRQAVTNADRTIRSIKREMGTDYKVDIEGKKYSPQEISAMILQKLKGD
                     AENYLGEKVTEAVITVPAYFNDAQRQATKDAGKIAGLDVKRIINEPTAAALAYGLDNE
                     KEQKIMVYDLGGGTFDVSIIEIGDGVIEVLSTAGNNRLGGDDFDQKVTDYILAEFKKQ
                     EGVDLSNDKMALQRIREAAEKAKKELSSATTTNINLPFITATADGPKHLDMNLTKAKF
                     DELTHDLVEKTAEPVQRALSDAGLQASELSKVLLVGGSTRIPAVQDKVRQLTGHEPSK
                     SLNPDECVALGASVQGGKLAGDAGAGDILLLDVTPLSLSIETMGGIATRLIERNTTIP
                     TKKSQIFSTAADNQTAVDINVVQGERQFAKDNKSLGQFRLDGIPPARRGVPQIEVTFD
                     IDANGIVNVSAKDLGTGKEQHITITAGSNMSDSEIDKAVKEAAEFEAQDKKRKEAIDA
                     RNDADSMVFQTEKAIEEVGDKLDANDKTAVEADLNALKDLIAKCNPEEMTDAQVADLK
                     AAQEKLMTSAQKLFAKLYEQQGGAAGAGAGTAGAGPDMGNAGAAGGHDDDVVDADYKE
                     V"
     gene            complement(19971..20561)
                     /gene="grpE"
                     /locus_tag="PLN77_01520"
     CDS             complement(19971..20561)
                     /gene="grpE"
                     /locus_tag="PLN77_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRM42993.1"
                     /translation="MEKETGAETDLPETEPAAEQEEAKKAAEAKEAEAAEETAEDGKE
                     RKFFKKKKDKKDEKIEELTDRLTRQMAEFDNFRKRTEKEKANMYAIGAKDIIEKILPV
                     VDSFERGLATVENPEEDPFADGMNKVYKQFTTALDELGVTVIEAVGKEFDPNLHNAVM
                     HVEDEEAGENIVVEEFMKGYMYKGSVVRHSMVKVAN"
     gene            complement(20632..21690)
                     /gene="hrcA"
                     /locus_tag="PLN77_01525"
     CDS             complement(20632..21690)
                     /gene="hrcA"
                     /locus_tag="PLN77_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRM42994.1"
                     /translation="MDTELDERKRKILKAIIQTYLETGEPVGSRTISKYTDLNLSSAT
                     IRNEMSDLEEMGYILQPHTSAGRIPSDKGYRLYVDELMREKEQEVADIKNMVIEKTDR
                     MEKVLQQVVKVLASSTNYATLITGPEYHRNKVKFIQLSKVNEEQLLAVIVVEGNLVKN
                     QMIDLDETINDEQILKLNLLLNTQLNGLTIEEINLGMIKKMKEQAGIHSGIISKVVDT
                     VAEAIAVSEEDVPVYTSGATNIFKYPELSDSSKASQLISTFEERQKLVDMIKGAAEEQ
                     DGEENTGIQVYIGNESSMEPLRDCSVVTTTYDMGNGMRGTIGIVGPKRMDYENVVDNL
                     KTLKVQLDAVFKKPDTGE"
     gene            21976..22194
                     /locus_tag="PLN77_01530"
     CDS             21976..22194
                     /locus_tag="PLN77_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM42995.1"
                     /translation="MKYQRLEDLRTDHDLTIRQVADYLGCNRDVYTRYEKGVRQLPIS
                     IAIRLAELYQVSLDYLVGISDEKRPYGS"
     gene            complement(22272..22637)
                     /locus_tag="PLN77_01535"
     CDS             complement(22272..22637)
                     /locus_tag="PLN77_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM42996.1"
                     /translation="MGKAERIEIEDFVQNIVERMETPEAFEKMISREEECEAQGQESR
                     LRDVLKKEWPVDEKGERIYQITNIYEEKAEELLIVELYTGIHLENGVPCGHFTLYLCG
                     EPDGWKLSETRMMEYLQNL"
     gene            22810..23028
                     /locus_tag="PLN77_01540"
     CDS             22810..23028
                     /locus_tag="PLN77_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM42997.1"
                     /translation="MKYQRIEDMRIDQDLTIKEISALLECNRDVYSRYEKGIRDIPID
                     YVIRLARFYDCSVDYLLSVSSKKRKFGD"
     gene            complement(23241..>25115)
                     /gene="uvrB"
                     /locus_tag="PLN77_01545"
     CDS             complement(23241..>25115)
                     /gene="uvrB"
                     /locus_tag="PLN77_01545"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRM42998.1"
                     /translation="VTGSGKTFTMANVIAQLNKPTLIIAHNKTLAAQLYGEFKEFFPE
                     NAVEYFVSYYDYYQPEAYVPSSDTYIAKDSARNDEIDKLRLSATSALSERNDVIVVSS
                     VSCIYGIGSPKDYMEMIISLRPGMEKDRDDVIRQLIDIQYDRNDMDFHRGTFRVRGDV
                     LEIFPAEETDKAVRVEFFGDEIDRLVQIDTLTGEILCELNHIAIFPASHYVVPMDTIL
                     KATGEIEKDMEEQVKFFKHEGKLIEAQRIEERTNFDIEMLKETGICSGIENYSRYLSG
                     LKPGEPPYTLMDYFGDDFLIIVDESHKTVPQIRSMYAGDQSRKSTLVDYGFRLPSAKD
                     NRPLNFGEFEDRIDQILFVSATPGDYEADHELLRAEQIIRPTGLLDPDVEVRPVEGQI
                     DDLISEVKKETEKHNKVLVTTLTKRMAEDLTDYMKEAGIRVRYLHSDIDTLERTEIIR
                     DMRLDVFDVLVGINLLREGLDIPEITLVAILDADKEGFLRSETSLIQTIGRAARNSEG
                     HVIMYADVMTDSMRLAIDETKRRRALQEAYNKEHGITPTTIKKAVRDLISISKEVAKT
                     EEKMEKDPESMSREELEKLIGQVQKQMKAAAAELNFEMAAELRDKMITLKKSLDDMEE
                     "
BASE COUNT         5264 a         6616 c         5029 g         8196 t
ORIGIN      
        1 ctgtgcaccg gcagcatcgt cctccccgtt cagcgcttcc gctgctttcg ggttcgcctt
       61 ttcggcttct tcctgtgtaa acgaggacag gcagtactcg catttatact ttccgcttgc
      121 cggatcgaag gtcagatcgc ctccgcagtt cgggcatttg tatgtaataa cctccatatt
      181 ctatccctcc ttccccggag ccgctgttgt attggatggt tatctgtttt cttttactgt
      241 cttggttttc cgcagttcga gcagaacttg ccggtattct cggtcccgca tgcgcagacc
      301 cattttcctg ccgcttccgg tttcgggctg ccgcagttcg aacaaaactt cccgctgttc
      361 tccgttccgc acttgccgca gatccacacc atcggctttg cacttccgca gtttgagcag
      421 aaattgccgg tgttctccgt tccgcaggag catttccata cgccttttgc agcttcctgc
      481 tgtgccttgg ctgccgcctg ctgctgcatc tgctgctgat tcgaagcgga cgccgatgca
      541 aacgaggaac cgaaggcatt catgcccatc tgaacaccca taaagccgtt catggctccg
      601 tttgggttgg ctgctgcttt ctccatactg cgtgccatcg ctccctgtac atagccctca
      661 cggatcgatg gatcagacat catcgcacct tcgttgcgca tctggatcag tttctgggaa
      721 gcttcatcgt aggaaaggct tgcgattgcc acggactgga tctccatgcc gcgcattgcc
      781 cgccagctct catccatcgc atctgccatg tagcgggaca gctcggcgct cttggatgct
      841 gcgaacgaaa tgcggatgcc gtcggctgcc atctgattga tcgaggactg caggccctct
      901 aaaaattcgt tcatatactg ctcgttgata tcctcgatct ctacgcggga tgcgtttctc
      961 ggcacagcct ctgcgtagaa gcggagcgga tcgacgatgc ggatggaata ggaaccgtgt
     1021 gcccgaagga acagctcggc attgtagaac tggtcgaaat agttgaccgg atttctcgtt
     1081 ccgaatttga ttcccttgat ttcctgcaga ttaatataga aaactctctg ctcatgcggg
     1141 gtctggccgc cgaatttcag gcgctcaaac gtatctttta ctgccgcgtc aagcgatccg
     1201 ctgaacatcg acggagacga tgactggtct acggtaaagt agcccggctc tgcggtgtaa
     1261 tcaatgatct ttccgccgtc caccagcatc atgaactgat tgtcatagac atggatcaca
     1321 gaaccgtttg aaatcgtctg gcttcccttt ttattctggc cttttcttac cagaattccc
     1381 tctgtaaata ccgtatgttc ccccatctca tacggttcca cggtctccag ccactgatcg
     1441 gcgagactgc cgtgtactgc attgactgct gctctgataa tacccatact tttacctcct
     1501 gtttgccttt ggcttcaaaa cagaaaggag catttccgtc ctctgttggt gaaaatgctc
     1561 cttattttac ttgtcactgt gttctgccca cagccgaatt atgcttcttc ctctttcgct
     1621 ccggttccga gggcaatcca cttcagatat gcgttgataa acagatcaat tccgccgtcc
     1681 atgactgcat cgacgtttcc ggtctctgcg ttggtacggt ggtctttgac catggtgtat
     1741 ggctgcataa cgtaagaacg gatctggttg ccccagccga tctcggttac ttcgccgcgg
     1801 atgtcgctca gcttatctgc ctgctcctgg agtttcagca tatacaattt tgctttcagc
     1861 atctgcattg ccttgtcctt gttctggaac tgggaacgct cattctggca cgtaaccacg
     1921 atgccggtcg gcatatgcgt gattcggatc gccgatgacg tcttgttgat gtgctgtccg
     1981 ccggcaccgc tggaacggta cgtatcgata cggatatcct catcacgaac ctcaacatcg
     2041 atctctttgt cgatatccgg cataacatcg cacgatacga acgatgtctg gcgttttcct
     2101 gccgcgttga acggagaaat acggacgaga cggtgaacgc ctttttcgga tttcagataa
     2161 ccgtaagcgt tttcgccgtt gacctggaat gtcacggact tcacaccggc ctcatcaccg
     2221 tccagaaagt cgagttcctc gatcgtaaag cctttgcgtt ctgcccatct ggtgtacata
     2281 cggtacagca tgccgcacca gtcgcacgcc tcggttccgc ctgcacctgc gttcagtttg
     2341 atgatcgcat tgtccttgtc aaattcgccg gacagcaggg ttttgatgcg gatggcatcg
     2401 aaggatgcct tgaactcttc catcatttcc gcgatttccg gaacaacgga atcgtcatcc
     2461 tcctcctgtc ccatctcgat cagagtttcc atatcttctt tggcggatac cagtttctga
     2521 tacgtatctc tgtcctcttt catgctgctc agctgtttca tctgctcctg cgcttcttcg
     2581 gggttgtccc agaaattggg ttcctccatt ttacgctcta attcttcgat cctctgctct
     2641 ttgttagcga ggtcaaagtg aatccctcac ttccactaat ggtttatcgt atgtgccaag
     2701 ttccattttg aactggtcta attcaactac cacaagcttc acctgctttc tgttttatat
     2761 tttgtatgac acgcagcttc tggtagggaa gctgcgtgct ttgtcaaacg gatcgtgctc
     2821 atccgtctca ctgctgttct gcttttatca aactggtttt cttccgcagc actgtttgta
     2881 cttcttgccg cttccgcacg ggcatggatc gtttggatag atcttacgct ctgcgcgctg
     2941 taccggtttt ctcgggccgg actcgtcttt gttggttccg gtcactttgg ctacctgctc
     3001 acgttctact ttctgctcaa cacgtacatg gaacaggaga cggacggtat cctgctggat
     3061 ggactcgagc atttcgttaa acatgtcgta agctgccatc ttgtactcaa ccttcgggtc
     3121 tctctggccg tatgcctgca ggccgatacc ctcgcggagc tgatccatat catcgatatg
     3181 atccatccac ttctggtcga taaccttcag aagaacgaca cgctcaagct cacgaatctg
     3241 ctcctcctcc gggaattctg cttctttctc ctcgtagagt ttaacagctt cctcttttaa
     3301 ctgctgtttc agctcgtttt tggaaatctt ctctacgcgc tcttttgtca ctgccttcac
     3361 cggcatgatg gagagcagca tctcatttaa ctcggtcaga tcccactcgg actgctcggt
     3421 gttctcgccg atgcagagat ccacagtatt gtcgatggtg tcggcaatca tcttgaagat
     3481 ggtgtctctc atgctctcgc cgtccagaac gcggcgtctc tctttgtaga tgatctcacg
     3541 ctgctcgttg ttgacctggt catagtcgag caggttttta cggattccgt agttgttgcc
     3601 ctcgatcttc ttctgcgctt tttcgatggc ggaagaaagc atcttgtgct cgatctgctc
     3661 attttccggt acgccgaggg atttgaaaat gcccatcagt ttttcggaac cgaagagtct
     3721 catcagatcg tcttccaggg aaatgtagaa acgggactca cccgggtctc cctgacggcc
     3781 ggaacgaccg cggagctgat tgtcgatacg gcgggactca tgacgctcgg tgccgatgat
     3841 ctttaagccg ccggcagctt ttgccacatc atccagcttg atatcggtac cacggccggc
     3901 catgttggtc gcgatggtaa cggctcctgc ctcaccggca tgggctacga tttctgcctc
     3961 gagctcatgg aacttcgcat ttaagacctg atgcggaatt ccctcgcggc gcagcatttt
     4021 gctgatcagc tcggaagtct cgatggtgat ggtaccaacc agaaccggct ggttcacggc
     4081 atgtgcctct ttgacttcct ctacgactgc gtggaatttc tcttttttcg tcatgtatac
     4141 ggcatcgtcc atatccacac ggatgaccgg gcggttggtc gggatctcga taacgtccat
     4201 gccgtagatg tcacggaact cctgctcctc ggtgagggca gtaccggtca tgccggcttt
     4261 tttctcgtat ttgttaaaga agttctggaa cgtgatcgtt gccagagtct tactctctct
     4321 tcttactttt acgtgctctt ttgcctcgat tgcctgatgc agaccatcgg aataacggcg
     4381 gcccggcatg atacggccgg tgaactcatc gacgatcaga acctcgtcgt ctttgactac
     4441 gtagtccttg tcgcggtgca tcagataatt ggcacgcagc gccaggatca cgttgtgctg
     4501 gatctccagg ttctccggat cggagaggtt ctcgatatgg aagaagttct cgactttctt
     4561 cacaccgtca tcggtcaggg taacgaattt gtccttctcg ttgacgatga agtctccggt
     4621 ctcctcgatc tcctcgccca tgatcgcggt catcttggtc atctcgccgc ttgcctcgcc
     4681 gcgctgcatc tggtgtgcca gaatatctgc ggtctcgtac agcttggtgg atttgctgct
     4741 ctgtccggaa ataatcagag gtgttcttgc ctcgtcgatc aggacggagt cgacctcgtc
     4801 gatgatacag taagcaagct ctctctgtac gagctgctct ttgtagatca ccatgttgtc
     4861 acgcaggtaa tcgaaaccaa gttcgttgtt ggtgatgtag gtgatgtcgc atgcatactg
     4921 ctggcggcgc tcatcgttct tcatatcgtt cagaacgaca ccgacggtca aaccgaggaa
     4981 tctgtggact ttgcccatcc actcagcatc tcggtttgcc aggtaatcgt tgacggtaac
     5041 gatgtgtaca ccgcgtccgg tcagtgcatt caggtaagcc ggaagggtgg aaacgagggt
     5101 ttttccttca ccggttttca tctcggcgat acggccctga tggaggataa taccaccgat
     5161 cagctgtaca cggtggtgct ccataccgag ggaacgtttt gctgcctcgc ggacggttgc
     5221 gaatgcttcc gggagaatgg aatccaggga ttcgcctccg ttgtagcggt ctttatattc
     5281 ctttgtcttg gcacgcagct cttcatcggt cagcgcctgc atggtcgggc ggagagcttc
     5341 gattttgtct acgattggat agattcgttt cagctcgcgc tcactgtgcg taccaaaaag
     5401 tttttctgtg aatttcatag aatgtttgct cctgctttct ctattaattg ccatattaac
     5461 atatggtatt attctaccac ttctggcaca agataacaat gaatttttcg taaacatttg
     5521 gtgccgttcc ccggttttcc gctgtttttg cggcttttcc cggactttcc ggccctttcc
     5581 gcgaaaaagt caccctgttt cacagctttt ctaagctgga aacagggtga ctgttcattc
     5641 tttatttact tttcagcact ccggctcgat cagaccgtag gtgttgttct ttctcttgta
     5701 aacgacgttt acttcgtcgg tctctgcatt gcggaatacg aagaaggtgt gtccaagaag
     5761 ttccatctgt acgcaggcat cttccggata cattggtttc atgccgaatt ttttcgtacg
     5821 gatgatctgg atgtcttcgt cctccagata gtcatgctcc agatattctt tctggaagtt
     5881 tcctgcttcc tgctgtttgt cggcaatttt gtttttgtat ttgcgaagct ggcgttcgat
     5941 gacttcctct accagatcga tggatacgta catatcgttg cttacctgct cggaacggat
     6001 gatatttcct tttaccggaa tggtaacctc aattttctgt ctttctttct ctacacttaa
     6061 ggttacaatg acatttgtgt caggcgtaaa atatttctcc agtttgccaa gtttttcatt
     6121 gaccgcggct tttaatccct ctgtcacgtc aatatttttt ccgctgatgg taattttcat
     6181 tatgcccaac ccctttgctc tcaaatttta caggtttttc acttcccctg taagtatctc
     6241 taattatagc aatagttgtc tttatttgca atagtattta gaaaattcat gaatatttta
     6301 gagttttttc tgttacagtt cacttgtatc ttatttttga cagatttcat gctcgcatga
     6361 aaatctgtcg agaataagat acaaagggag cgcctggtta tgagcaaaag gagctgcgca
     6421 gcagcggaga gcgctgaaag cgcggttttg cgaatggcgc gggtgaacgt ccgtttccgc
     6481 ttaccggcgg catactgcca gtacgtcttc aaatttcatg tttccgccaa acagatccag
     6541 tgcgcttccg atcgtcacat ccacatgatc gttcccaacc tttttcagca gttccagatc
     6601 cgcaaagttt ccgacgccgc cggcatatgt aacagggaaa tctttcattt tcgcaagtaa
     6661 ttccaccagt tctgtctcga taccggatgc ttttccttcg acatctaccg cgtggatcag
     6721 aaattcatca cagtattccg tcagatgatg caggagcggt tccgttactt cttcctctgt
     6781 aaacttttgc cagcggtccg ttacaatata atatttcccg tttcgtttcc ggcagcttaa
     6841 gtccatggtc agccgctgtt ttccaacagt cgttaccagt ttttcgagat tttcatagaa
     6901 tacatggcca tcccggaaca catacgatgt cacgatcaca tggcttgcac cggccgtcag
     6961 aaattcgcag gcattttccg cgtcgatacc gccgccaacc tgcagtccgc ccggatacgt
     7021 agaaagtgcc agcagcgcct gccgctttgt ttccggataa tattcggaat cttttttgtt
     7081 cagaagaatc acatgacctc cggtgatccc tttttcctca tataactgcg catagtaaga
     7141 cgcatcctgc tccgaaacaa aattttcatt tgcctggttt ccctgatctt ttaaggatcc
     7201 cccaacaatc tgttttactt ttccattgtg gatatcaata catggtcgaa atttcataaa
     7261 taaagtcccc tgtccctttc ctgttcatcg aatggatatc tgtagttagc gatgattcgg
     7321 ttaaaaaatt tttatctccc tcaaatttct catattatat caaatttcgg acttcctgtg
     7381 tgcaaattca ctcttttttc actaaaaagc tccccttgga tcttttgttc gtgtgcatga
     7441 cagcaaaaaa atgccgcaat ccggattttt tcccgaactg cggcatccca ttatacttta
     7501 tcttatgttt tgtcctatcc cgtgcttatg cctagactca tgtttcagtc tatccttggg
     7561 tttatgcctg gaatcatgtt ttctactcct gtaccacaga ataagaatag tcagaaagat
     7621 tgacacggat caccgtttct tctccggacg gtgtaccgtc cattttcaca tcaacatgat
     7681 caccttcgta atgtacttca tatgcccaat agtatcccgg atagaaactt tctacttcat
     7741 acaattcatt tccgtcttta tcaattgcgc caaaatccgg tccatagaaa gagcagtaat
     7801 aaagtgttcc gtccggtccg aaatccgttc ctgtcatgga accatgtgat gatgcactgt
     7861 gccagatttc ttttccgtcc cggaggcgta cggcataaat aactccacaa tgcgcataat
     7921 aatacctgtc ttcataaatt ccaatttctg tattggctgt tatctccgct gccggagatt
     7981 tttctgtctc aatgggccat actacatttt catcctgatc caatccggta atcacagcat
     8041 actcctgatc ccgattttct tctgaagcaa acaggtcatg cgttttttct ccgttattat
     8101 aaaaggtttc cagtattaca tgtgttgcat ctttttttgc caccttttcc agggcagctt
     8161 tcagattcag catcggatac tcttcccgct tgtcctcctg acaggttgat cttgcggttc
     8221 cggctgtgga gatcagtgtc tcttttacct cctccgccga aagttccgga gccacagacc
     8281 acagaacagc cgcagaaccg gaaaccagtg gggccgccat ggatgttcca ggcagatttc
     8341 catatttaat cccgtttttc actgttctaa gattttcatg gcctggattt tcttcggtat
     8401 aggaatcatc tttttctgta atcgtgctgt aaacatcata tccaggtgca acaatatcta
     8461 tattggaacc ataatttgaa aattctgtca tttgatatcc cttttctgtt tcctgcactg
     8521 cacctacaat caggatatga tttcgaattt tctcatagga gtatccgctt ctgcttaatc
     8581 tatgcgtttc tccatccagt ctgttataaa cctcctcatt aattccgcaa taatctcctg
     8641 tcttttgaac atcaaatccc acaccaccgt tatcataccc attgcctgca ctctggacaa
     8701 ttaaaaatct ttcctgttga tttaaaagca attcaaaaag catcactatc atctgactgg
     8761 aagtgtattt tgcgctcgtt tctaattcct tttgataact ttcgtacaac tcatatttct
     8821 gatcaatctt ccctttttct atgagaaact ccgctatttt ttcttcccat accggtcttt
     8881 ccccgattag cgtatctttt aaatactgct ctctggattg tatcacagtt gcccatgaat
     8941 tattcaccac gcaggccgta tgttcactta gcatcgtttc aatcatctgt tttgttacat
     9001 ttacatattc tcctgtactg aagagatctt ctttccacca gctcacaaaa tacaaatccg
     9061 tccgatctgc cacaccacgg attccggcct gattattttc cgcaccgatc aatcccgtca
     9121 catgtgtgcc atggtcaaat gccagatttt ctttgtaatc ttccagccag tgaattttac
     9181 tttcgccgtt tttctgaaat tcttcgtgtt ccgcatcaac tccatcatcc ataatgccga
     9241 ccgccacatt ggcaagatcc tgctcgtggg aatcaacata gtcccatgca gaaaatgctc
     9301 caattgcctc tgcccaccag tcatttccat ctggtttgct ttcattatgg tcattattct
     9361 ttttatcctg ttctcctgcc tcccatggat tctcatctgc agtattttcc atcatcatcg
     9421 gaatttccgt cgaactgtag agcacatttt cttcttccat cagaatttca gccttctgtt
     9481 ccagatccgc atacgcggtg tcctccacca ggatttcaat cacgttcatg cttccgctca
     9541 tatttccaac cagcgttcca tctacctttg ctgcaagtgc ttcccgctct tcctggctgc
     9601 tgttttctat cagataaaca atcagcagat tttcgtagta caacaggccg gtatcttcat
     9661 cataagaaac ggtacttacc gacggctgat aaaccgtatc cccattggca cattttttca
     9721 ttgtgcctgg ctctttcaca tcctgactat cctgcgactg ttctccattc tgattcttgt
     9781 tctgtttttg gctttggtca ccttcatctg cctgcacttt ttcctgtttt tgtttccatt
     9841 cttccagttg tctttctttc catccctgta tttgcttctt ctgtgatcca actgcaatgg
     9901 caagcaggat acctaaaaac agcacaatca caatgacaag taccagaaat cttcgaattt
     9961 tcttttttct cttcttcttt ttttcttccg ccacatggca gccacagtac tggcaatagg
    10021 catcttcttt ctgaatcctg cgtctgcacc tgggacattt gatttccttt ttcattttct
    10081 gctcctctta ttgcagtttt gatccgcagc gcgtacaaaa cttttctcct tctttcggaa
    10141 ttcttccgca ggagggacac gctgaccgca tcttcgaagc tgccgggttc tttgatacgg
    10201 tttcttccgg cttgattttt ttcgattccg ttttttttga tttgattttt tccggctcaa
    10261 tttttcttga aacacttttc tttgaagcac ttttctcttt agaaaccatt ccccacagtg
    10321 caaatataag aatcagaaca tgaagtgcta tccacaccca cacaaattcc atgcgaagcg
    10381 gcattcctgt catctggccg ttataatcaa ccttgttttt ccatccccag ctgagtcgtt
    10441 gcattgacca cgcaagacct ccgtttgtca cgatggataa tacaattgcc agaacatagg
    10501 tcttttttcc atttaacagc gtaaccagaa ctgatcccac aagcgttact gccgctgttc
    10561 caatcaacca tgcgttgata ttttcaaaga cactttcctg catcagtttc cagatcatcc
    10621 ctccgacaaa agagtctcct tcctggcttt ccattccata gataatcaca tcgtaaagcc
    10681 ggatggcatc atctttccag ccgatcattc tgcagaaata tagtggtatc gtacataaaa
    10741 acagaattac aatgacgtat tttgcttttg ttttcatctg aaaattctcc tgttctgctc
    10801 tatttttata cttcttcgga tggttccagc accattttgc tatctgctgt atccatttcc
    10861 gaaaataact ttttgccttt cagatcgtaa taatctgtct tatctacctc gacatattcc
    10921 gactcaccat ccacagtctc tttttcttcc tcgctgtatg ttacctgcag gattccatca
    10981 ctgaccagtt ctcctttttt tccatcctca acggtatata acagattccc gcttaaatca
    11041 tacaattctt tctgatcgat cagtaatgtc ttatcttcca tggaaaaaga ttctccggca
    11101 atcggatcgg caatttcttt catggtatcc gcattgatca ggcagacata aaactgatta
    11161 tcttccccat acatggaaag tgcaagaaca ttatcctcga tgcagcttgt tgtagtggaa
    11221 taatatttaa aatacggcgc atactttcct gtatattttt tgatatcgcc tgtctccaga
    11281 tcacacaaaa aataaatgtc ctggtcgtcc tgtcttaccg taaaaagtgc atattttttt
    11341 gtccagtcga gcagagtcgg atatccgcca aacgggccaa gatactgact cgggatttca
    11401 atttcttttt cttctccatt cccgatcact gtaatatgaa acggattttc atcccctgcc
    11461 caatatccat cgtccattgc gcccgtttct atgataagtc cgtcccgcat tccatgttct
    11521 tctgctttgt caaacgaaac gtttaactct gtttcttttc ccagttccgg ctcaaccaga
    11581 acacagactt ctttcgtacc gtccattttt ttatacagaa attttccatc acccatgtag
    11641 gatcttttaa agctcagatt ttccaggtcc tgattgctga ctgtataatc acacacttca
    11701 cccccttccg gactatacag tgtatatttc caaaacccgg catcatccca ggatacattt
    11761 tcttcctcct ccagccaggt ataaccaccg ccgtaatctg ccactttgtc tgcatcatac
    11821 tggcttttta tcattccatc cgtgtcaatc acataaaaaa tattgtcata gacgaaccag
    11881 ttataaccat tattaaaatt cacatcgtac cgataaatat ctgcatcaat tgcatcttca
    11941 tcaaaaggta tataaaactt taactttccc tttttatcca gaaagccttt gtaagattcc
    12001 ccctttccgt gaattcttac aaaggcaagt ccatcctgaa attctcttgc atcagaaaat
    12061 ttcatatttt ccaatgtatg agacgtctcc gcatttgaac ttatatcgtc ttcatcatcc
    12121 gaccatgtat catcatcatc ttcttcttct tcgtcttcgt cgtcttcgtc gtcatagttc
    12181 ttccggttct atgccagatg cactcacttc cacaaacaca tttcttgcat cggtgaccgt
    12241 atctccttct tctccataat gactttttat tttttccatg ttgccttcgg actgtttttc
    12301 tgatctgact ggccaatggg taatttgtat atccccaact tcatcttgca gtttctgaag
    12361 ttcctcttcc actgcatttt ctatgatcca ctgtaacgtt tccccatatt ggccaaaata
    12421 ccgctccggc aacaggctca gaagcgtatc ggtatctcct gtaaataatg cttcgactgc
    12481 acgatctgcc acctgcatat attcctcatc ggactcctgg ctttccgctg tttttccagc
    12541 tttttcccct gattccgtca tctggagtat tccatattcc ggaacaaact tccatggata
    12601 taaaattgct atagccgcca taattccaaa agtaatgatc aaaattcggc ttcctgcttt
    12661 tttcatatat tctctactcc tttttctctc atcgtatctt tacttctgaa tagagtccta
    12721 atataatttt ccagattttt tgcactctct tactctgtaa ggacactgct actctgcttt
    12781 ccacaatagg taatagggat ctcttctcac cagtccagcc ggaaattctg ttccgttttc
    12841 atttcatccg cataccagaa atctactata ccgattccaa tccgctcact ttcttccttt
    12901 tgataaagca tcagatcatg ccggctgtca tacacaatat ctccatcctc ttcccgaatc
    12961 gccttcgtga ctccatctgc aatttttgtc ttatttcctt ttttatcaaa aatggaaagt
    13021 tcatattctc catcggaatt ccgatccgtg taagccatga ctgttccatc ttcgtaaatc
    13081 ttgacatagt cttccagcac ttcttttgca aggcatctgc tttttccatt ttctatcacg
    13141 ttgacatcgt agtacgcatt gtccccttcc gtgtagaaat tgcttgtata ataaatctct
    13201 gttggcgtaa cagattttac atagccttcc ggcatctctt ccagatcgct gacttcatca
    13261 tttttgattg cggcgtgcca gaaatcagat ccgctttcca ggaacagatc tgtttcgttt
    13321 gcatnnnnnn nnnncagaag ttttctcctt cctcatctat gtcttcaatt tttttcgcaa
    13381 cttctcctat gatgtgaatg gaattcttcc tgctcgttgg tttaacatac attccctgct
    13441 catatattct ctgatctaaa aggtcaatca tataatctgc atccttcact tctgctaaat
    13501 ctaccggctc cattttcttt acatccaggt atagtacgct atttccccta taaagtccgg
    13561 cattattaat gtcattggct gtaatctgtt ttatttcacc atctttatat tcataaagac
    13621 tttgacactc cgttggaaca ggatgatatg attccgcttc tttttgtaat tcctgccact
    13681 gttccgtgtt tcctctgctg tcaatcacga agtcttccag ttcacattct gtattctcgc
    13741 cggaagcaaa gtaataaacc gcgccatttt cccatgcata catatcactg actggtgttc
    13801 ccagctcttt tgtctcttcc tcaaaagatg ttagaaaaac aagctgatca tcctctgact
    13861 gaccgtcata tcgtctgcac aaaatatgct cctgatcatc tgccagcata acatcggtca
    13921 tcccatccgc cagaaatact tcttccgttg gattgttata gcttacgcca cagacatcaa
    13981 atccttcgtc agcgtttccc ttcgtataaa cgagtcccgt tccgtctttg agctgataat
    14041 atccctgcag tgaatctgca atcttcacag atacttcccc gtcataacaa aacagctcgg
    14101 aatccttgtc gatataaagg agttttccgt tttccagcgg ataaaaaact ctgacatttg
    14161 ccgcaacttt tgttttgtat ttctcatttt tttcttcttt tttggacaac ttctgatatt
    14221 cacaccggta gagtctggac gttcccgtcg aaatatcata gttttccata taatagagat
    14281 attttcgatc cggtgaaagc tgcattacgg catcgcccag ccagtccagc ccattgtctg
    14341 taatggttat ggtatgtccc agtttttctt tcgtgagaat ttcataactg tccccatata
    14401 attctacaaa tgggtgttcc atattttccc cggacttcac acagaaaact actgctccga
    14461 ctgccactat tgcagtcagg ccaagtgctg tcgcaatttt ccatcctttt tttctgcgtt
    14521 ttaccggcag cacggcgtcc gatttattgt attctgctct tttacgttca gctgtgtttt
    14581 tttcttcttc tttttcttct gctatctttt tattttctgc tgtttccctc gttttcggtc
    14641 tgacaggttc tatcgttttt ttcacactcg gtttgaccgg ctctgttttt tttctctgct
    14701 cctctttccc atcatccggc actatatctg gcactacaat atttccatcc tccagttttg
    14761 ctccacagct tccgcaaaat cttgtcgcat ctgaccactc ttttccacat accgggcatt
    14821 ttttcatact tgttttcctc tcctgtccct ttttatccac aaatcttttt tctttgcaat
    14881 aaatgatcca aaatccacct ccctattttg gatcatttat ctttttgctg tctgatttga
    14941 tcttctcttc tgtgctttgc tttttcaaac acgctctcga ttttactcgt caactttatg
    15001 tccacaaacc gtacagaatt tcatatccgg ttccagcggt gctccgcatt cactgcaaaa
    15061 agccggccgt tcctgttctt cttccaccgt ttcctgttct tcactctgca gtttcattcc
    15121 acagctgctg cagaacatca tttccggttt gatttttgca ccgcagcgcg ggcagctgtt
    15181 cattgcattg attgcctgga tctgcagttt gcatgcttcc atagcctctt ccagctcccg
    15241 gatctggcca atttcctctt tgacttcttc cagcggattt tcccgatatg cacagtaaac
    15301 tttatatcct atttcactgt agatttcatt gattttcgtc tccgattcag aaaccgccat
    15361 attcagtttc gtaatctccg acaaatcctt tgctttttgc atagttgaat atcctgcttt
    15421 tgaaattttt ccttttactc catctaaaaa tgccattttt cttccccttc catttctttt
    15481 ttgtacttta taatctgctt tttttagatc tgccgtcttc tctcttataa ggggctttcc
    15541 cctcctgatt cacgcaattc cccgcctcac tgcttattgc ttttctcaat gaaaacaggg
    15601 atttccttga tttgattaaa tttttccgct cagcgcgcat cacttttcga ttcgtttttc
    15661 tatttttctt ccttatagct tttcaggaca cggctcagac tctccggcga taaatgcaga
    15721 taggaagcga tatctttctt actcacaacg tctaccagat cccgataggt atcaacaaaa
    15781 tattgatatc gttcctttgc cctgtggttt gcaacaatat cctcatgttc aattagcttt
    15841 ttatatgcaa ggctgagtgc acggttatat aaacgcatca tttccggcga ttgctcaatc
    15901 aacttcaaca attctggtat cgggacgcac agcatggtac acgaactgat tgattctatc
    15961 gtcaaaagtg atttcacatt tacggataaa ttggaaattc ctacaacaat gtctcctttt
    16021 aaataaccaa agcaggaaac atgctctttt ccatctccct tgctttcaca ataccctttt
    16081 acaatgcccg atatcagaaa aggaacgcat ggcagcggtt ccccttctcg aatgataact
    16141 tccttatctt tcaccttcca gacatacgcc atttgttcca gactgctcag cacattttta
    16201 tttttgatac ctaaataagt tttgaagaac tcctgttttt ctatattttc attttccaac
    16261 cgctctattt cctttctgcc tttttatttt acattacata acatcaatct tttttattcc
    16321 tatgcaattt tgttaattat atcatgaaca gtttttcaca tctcatcatt ctcttgattt
    16381 agatcaatat ttgcaatttt ctgaatattc tgtattttta aattttgtca aaaaagaaga
    16441 acctgtttct cctgcaggtt cttcttttca tttatctcat attttctatt tctttcatat
    16501 tagagcgtgt tggaaaagac ttttcatatt ctacatgctc aagtctgtga gaaattttgt
    16561 ccgaatgaga acaaaaacag atagattttt tatttatcaa tatcctcaaa cgcttccttc
    16621 accttatcca taaacccttt tttcttcttc ggctgttctg cggtattctc accatcggct
    16681 gcttttgctt ctgctgccgg gcggtttccg caggcttcgt cgaaggcgcg gagggcttct
    16741 tttgctttct cattcagttt cgtcggagtc tggactacga gtgttacgta gtgatcaccg
    16801 cgcacattgc tgttgcgaag ggacggaaca ccttttccct tcagacggat cttcgtatcg
    16861 gtctgggttc ccggttttac agtgtagaga acgtctccgt caacggtgct gatcttcaca
    16921 tcgccgccga gtgctgcctg ggcaaacgta atcggtgcag tggagaaaat gttcatatcc
    16981 tgtctctgga agatcggatg acgggttacc tgaacctcga caagcagatc tcctctcggt
    17041 ccgccgttta cacccggctc acctttttca cggatccgga tgctctgtcc atcgtcgatt
    17101 ccggccggaa tgttgacctg aatgcgtttt ctgctggatg tatagcccgt gccgtggcat
    17161 gcggtacatt tatcgcgaat gatctttccg gttccgttac aatccggaca cgttgtcacg
    17221 tttctcacag aaccgaacat ggtctgctgt gtgtacgtaa tctgaccggt accgttacat
    17281 ttggagcatg tttccggtga agttcctggt tttgcgccgg ttccgtggca cgttgtacag
    17341 gtatccttca ggttgatctc gagctctttc tggcatccga aaacagcttc ctcaaacgta
    17401 atgtggacaa cagcgcggag gtttgctccc ttcatcggtc cattgtttgc tcttcttctg
    17461 cttccgccgc caccgaacag gtcgccgaaa atatcgccga agatatcacc catatctccg
    17521 ccgccaaagt cgaagccgcc gaatccgccg gctcctgcgc cgccaccgcc gttttcaaat
    17581 gcagcgtggc caaactgatc gtactgacgc cgtttgtccg gatcactcag cacgccatag
    17641 gcttctgtcg cctctttaaa ttttgcctcg gcctctttgt cgcccgggtt catatccgga
    17701 tggtatttct tggccagttt acggtatgct ttttttaatg ttgcatcgtc cgctccgcgg
    17761 tctacgccga ggacctcgta ataatctctt ttttctgcca tttatgttca ccttttcatt
    17821 tctgttccac aaatttcatc tttgcagaaa tgtttttccg cacgctccag tatagcactt
    17881 ttccgaaagc aataccagca taaatacagg gggcggaatg tattttccgt cccctgcaaa
    17941 cattcgcctc acagggcttt ttgcgtttat ttacttattg tttcaactca cagtcggtat
    18001 tttcacggag tgtgacatgc tgaaaattaa acctctttgt agtcagcgtc tacgacatcg
    18061 tcgtcatggc cgcctgctgc tcctgcattt cccatatccg ggcctgcacc agctgttccg
    18121 gcaccagcgc ctgctgcgcc gccctgctgc tcatacagct ttgcgaacag tttctgagcg
    18181 cttgtcatca gtttttcctg agctgctttc aggtcagcta cctgtgcatc tgtcatctct
    18241 tccggattgc attttgcaat gaggtctttc agtgcgttca ggtcagcttc tactgctgtc
    18301 ttatcgtttg catccagctt gtcgccgact tcctcgattg ctttctcggt ctggaatacc
    18361 atagagtctg catcgtttct tgcgtcgatt gcttctttgc gtttcttatc ctgagcttcg
    18421 aactcagcag cttctttgac tgctttgtcg atctcgctgt cggacatatt agagcctgcg
    18481 gtgatggtga tgtgctgctc ttttcctgtt cccaggtctt ttgcagatac atttacgata
    18541 ccgttggcat cgatatcaaa ggtaacctcg atctgcggaa cgcctcttct tgctggcgga
    18601 attccatcca gacggaactg gccgagggat ttgttgtctt tcgcgaactg acgctcaccc
    18661 tgtactacgt tgatatctac tgctgtctgg ttatcagctg cggtggagaa gatctggctt
    18721 ttcttggtcg ggattgtggt gttacgctcg atcagacgag ttgcaatacc tcccatcgtc
    18781 tcgatggaca gagacagcgg agttacatcc agaagaagga tgtcgcctgc gccggcatct
    18841 cctgcaagtt taccaccctg tacggaagca ccaagtgcta cgcactcatc cgggttcagg
    18901 ctcttgctcg gctcgtgtcc ggtcagctgt cttactttat cctgtacagc cgggatacgg
    18961 gtagatccgc ctaccagaag gactttggaa agctcggatg cctgaaggcc tgcatcagaa
    19021 agtgctctct gtaccggctc tgcggttttc tctaccaggt cgtgggtcag ctcatcgaat
    19081 ttagcctttg tcaggttcat atcgagatgt ttcgggccat ctgcggttgc agtgatgaac
    19141 ggcaggttga tgttggttgt agttgcgctg gaaagctctt tctttgcttt ctctgctgct
    19201 tctctgattc tctgcagagc cattttatca ttggaaagat ctacgccttc ctgtttcttg
    19261 aactcagcaa ggatgtaatc tgttactttc tggtcgaagt catcgccgcc cagacggttg
    19321 tttcctgctg tggaaagaac ttcgataaca ccgtcaccga tctcgatgat ggaaacatcg
    19381 aaggtaccgc ctccaaggtc gtataccatg atcttctgct ctttctcgtt atccagtccg
    19441 tatgccagag ctgctgcggt aggctcgttg atgatacgtt ttacatcaag acctgcgatt
    19501 ttaccggcat ctttggttgc ctgacgctgt gcgtcattga agtaagccgg aactgtgata
    19561 actgcttctg ttactttctc accaaggtag ttctctgcat cacctttcag tttctgaaga
    19621 atcatagcgg agatctcctg cggagagtat ttctttcctt cgatgtctac tttataatca
    19681 gtacccatct cacgtttgat ggaacggatg gttctgtctg cgtttgttac agcctgacgt
    19741 tttgccggct cgccgaccag acggtcacct gtttttgtga atgctacgat agatggagtt
    19801 gttctttcac cctctgcgtt tgcgataacg gttggttttc caccttccat aacggctaca
    19861 cagctgtttg ttgttcctaa gtcaatacca ataattttgc ccattgttgt ttcctcctca
    19921 atgaattatg ctaaaaaatg gtttaatttc tatttattta tcttcaaggc ttagttagcc
    19981 accttgacca tactgtgacg gactacggaa cctttgtaca tgtagccctt catgaactct
    20041 tctacgacga tgttctcgcc ggcttcctca tcctccacat gcatcactgc gttgtgcagg
    20101 ttcggatcga attcctttcc aacggcctcg atcacggtta caccgagttc atcgagtgct
    20161 gtggtaaact gtttgtatac tttattcatg ccgtctgcga acggatcttc ctccgggttt
    20221 tccaccgttg caagtccgcg ctcgaagctg tcaacaaccg gaagaatctt ctcgatgatg
    20281 tcttttgctc caatggcata catatttgct ttttcttttt cggtgcgttt gcggaagtta
    20341 tcgaactctg ccatctgacg ggtcaggcgg tcagtcagct cttcgatttt ctcatctttt
    20401 ttatcttttt tctttttgaa gaactttcgt tctttgccat cctcagcagt ttcttctgca
    20461 gcttccgctt cttttgcctc tgctgctttc tttgcttctt cctgctccgc agccggttcg
    20521 gtttccggca gatcggtctc tgctccggtt tctttttcca tctcgcgtgt aggttcttcc
    20581 atggtttcct ctttctttct attttcttcg ctcactgatc attccacctt tctactcacc
    20641 tgtgtccggc tttttgaaga cggcgtccag ttgaactttt aatgttttca ggttatctac
    20701 cacattttcg taatccatcc gctttggtcc cacgataccg attgttccgc gcataccgtt
    20761 tcccatatcg tatgtggtgg tgaccacgct gcagtcccgt aagggttcca tcgaagattc
    20821 atttccgatg tatacctgaa taccggtatt ttcctctccg tcctgctcct cggcagctcc
    20881 tttgatcata tcgaccagtt tctgccgttc ctcgaatgtg gagatcagct ggctggcttt
    20941 gctgctgtcg gaaagctcag ggtacttgaa aatgttggtt gcgccgctgg tgtacaccgg
    21001 cacatcttct tcactgactg cgatggcttc cgctacggta tcgaccactt tgctgatgat
    21061 gccggagtgg atgcctgcct gctctttcat cttcttgatc atgccaagat tgatttcttc
    21121 aatggtcagg ccattgagtt gtgtgttcag aagcaggttc agctttaata tctgttcatc
    21181 gttgatggtc tcgtcgaggt cgatcatctg gtttttcacc aggttgcctt ctacgacgat
    21241 gacggccagg agctgctctt cattgacttt tgagagctgg atgaatttca ctttatttct
    21301 gtgatattcc ggtcctgtaa tcaacgttgc atagttggta gatgaagcga ggactttgac
    21361 aacctgctgc agaacttttt ccattcggtc tgttttttca atgaccatgt ttttgatgtc
    21421 tgcaacttcc tgttcttttt cccgcatcag ctcatctaca tagaggcggt agcctttatc
    21481 ggaaggaatg cgtcctgcgg aagtgtgggg ctgtaagatg taacccatct cttccagatc
    21541 ggacatttca ttccggattg tggcggagct taaattcaaa tccgtgtatt tggaaatcgt
    21601 tcttgagccg accggctcgc ccgtttccag atacgtctgg atgatcgctt ttaatatttt
    21661 tctttttctt tcatccaatt ctgtatccac taagataagc tccttcctgt tgtatagtcc
    21721 tgttagcact cgtttcagtg gagtgctaac atctatgctc ttaagatagc acgctgcctt
    21781 ttgtttgtca acaccttttc ccggtttttc tatgaagaaa ttgtgaaaaa agtataaacc
    21841 gttttctttc tgaaaaggaa tctgactttt cttttttatt gcattctact gtttcaaagg
    21901 cgctatagcc gtatggtagg attctgccat tttgatacga ccatttagta gcatttttct
    21961 cagaggtgat gaatcatgaa atatcagaga ctggaggatc tgcgcacgga ccacgatctg
    22021 acgatccgcc aggttgccga ctatctcggc tgcaaccgcg atgtgtatac ccgatatgaa
    22081 aaaggcgtcc ggcagctgcc gatctccatt gccatccgtc tggcggagct gtatcaggtc
    22141 agtctggatt atctggtcgg catcagcgat gagaagcggc cgtatggatc ctgactttcc
    22201 ggtgtctccg ccgcagcaaa agcaggagga cagtttttct tttccctgtc ctccctgttt
    22261 tgctttttcg tttacagatt ctgcagatat tccatcatcc gcgtttcgct gagtttccat
    22321 ccgtccggct ccccgcagag atacagcgta aaatgcccgc acgggactcc gttttccagg
    22381 tggatgccgg tatacagttc tacaatgagg agttcttctg ctttttcttc atatatatta
    22441 gtgatctgat agatgcgctc tcccttttcg tccaccggcc attctttctt cagcacatcc
    22501 ctcagacggc tctcctgccc ctgcgcttcg cattcttcct cccggctgat cattttttca
    22561 aacgcttccg gcgtctccat ccgttccact atattctgga caaaatcttc tatttctatt
    22621 ctttctgctt ttcccattcg ttcccccatt tctgccgtac atatttttct gttttctgca
    22681 caggctgtct tcacccgccg tttctctctc ccgccccatt ctccaatttg cgaccatatg
    22741 atctgattta actttgtcca tccaaccgtt tggtagtatg ggtttgaatc tgaaaggatg
    22801 gtacaacgta tgaaatacca gagaattgaa gatatgcgga tcgatcagga tctcaccatc
    22861 aaagagattt ctgccctgct cgaatgcaac cgcgacgtct attcccgcta cgaaaaagga
    22921 atccgcgaca tcccgatcga ctatgtgatc cggcttgcac ggttctatga ctgctcggtc
    22981 gactatctgc tgagtgtctc cagtaaaaag agaaaattcg gcgattaatt tttttattgt
    23041 tgtctttttt atttttgtgt tttgcgtttt gatacgaccg tttggtcgta ttttatatta
    23101 taatggtatt cttattttgc gtcaattctg aacaagaagg aagcaaaatc ggacgcaaaa
    23161 aaagcgtgcc ggaaagtttt ctgtcgcttt tccggcacgc tttttttcat gctcttttct
    23221 tcttttttat ttctgatttt ttattcttcc atgtcatcca ggctcttctt cagtgtgatc
    23281 attttgtcac gcagctcggc ggccatttcg aagttgagtt cggcggcagc ggctttcatc
    23341 tgtttctgta cctggccgat cagtttttcc agttcttcgc ggctcatgga ctccgggtct
    23401 ttttccatct tttcttctgt ctttgccact tctttcgaaa tgctgatgag atcgcggacg
    23461 gcttttttga tggtggtcgg agtgatgccg tgttctttgt tgtaggcttc ctgcagggca
    23521 cggcggcgct tcgtctcgtc gatcgcgagg cgcatggaat cggtcatcac atcggcgtac
    23581 atgataacat ggccctcgct gtttcgcgcg gcacggccga tggtctggat cagggaggtt
    23641 tcggaacgca ggaaaccttc tttatcggca tccaggatcg ccacgagtgt gatttccggg
    23701 atgtcgagac cctcacgcag aaggttgatg ccgacgagca catcgaagac atcgagacgc
    23761 atatcacgga tgatctcggt ccgttccagg gtgtcgatat cggagtgcag gtagcgcacg
    23821 cggatgccgg cctctttcat atagtcggtc agatcctctg ccatacgctt cgtcagcgtc
    23881 gtcacgagaa ctttgttgtg tttttcagtt tctttcttta cttccgaaat cagatcatcg
    23941 atctgtccct ccaccgggcg cacctcgaca tccgggtcga gaaggccggt cgggcggatg
    24001 atctgttcgg cgcgcagcag ctcgtggtct gcctcgtagt cgcccggcgt ggcagatacg
    24061 aagaggatct gatcgatccg gtcctcgaac tcgccgaagt tcaacgggcg gttgtctttt
    24121 gcggacggca gacggaatcc atagtccacc agcgtcgatt tccgcgactg gtcgccggca
    24181 tacatgctgc ggatctgcgg aacagtctta tgcgactcat ccacgatgat gaggaaatca
    24241 tctccgaaat agtccatcag tgtgtagggc ggctcgcctg gctttaatcc ggagaggtaa
    24301 cgcgaatagt tttcgatgcc ggaacagatg ccggtttctt tgagcatctc aatatcgaaa
    24361 ttggtccgct cctcgatgcg ctgggcctcg atcagttttc cctcgtgctt gaagaacttc
    24421 acctgctctt ccatatcctt ttcaatctcg cctgttgctt tcaggatcgt gtccatcggc
    24481 acaacgtagt gggaggcagg aaagattgcg atgtggttca gttcacataa gatttcaccc
    24541 gtcagcgtat cgatctgcac cagacggtca atctcatccc cgaagaactc gacgcgcacc
    24601 gccttgtccg tctcctccgc cggaaaaatc tccaatacat caccgcgcac gcggaacgta
    24661 ccgcggtgga aatccatatc attccggtca tactgaatat caatcaactg ccggatcaca
    24721 tcgtcccggt ccttttccat tcccgggcgg agcgaaataa tcatctccat gtagtccttc
    24781 gggcttccga ttccatagat acacgaaaca ctggaaacca caatgacatc gttccgctca
    24841 ctcagtgcag aagtcgcgga aagccgcagc ttatcaatct catcgttccg cgcagagtcc
    24901 ttcgcaatat acgtatccga agacggcacg taagcctccg gctggtagta gtcgtagtag
    24961 gagacaaaat attctactgc attctcaggg aagaattcct taaactcacc gtacagctgt
    25021 gctgcaagtg ttttattgtg cgctatgatc aaggttggct tatttaactg tgcaataaca
    25081 tttgccatgg taaaggtctt gccggaacct gtaac
//
LOCUS       DAOMUF010000010        24390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_303_length_24390_cov_3.342305, whole genome shotgun sequence.
ACCESSION   DAOMUF010000010 DAOMUF010000000
VERSION     DAOMUF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_303_length_24390_cov_3.342305"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..906
                     /locus_tag="PLN77_01550"
     CDS             262..906
                     /locus_tag="PLN77_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HRM42999.1"
                     /translation="MMEYTSKYWNMLTPLIKKSLLKRYGKEETAELLRKTNQIYRDML
                     RRADDIGKDNPMASNLYEGLIFLALWDASDRQISVDELRTISEEMMAFPLLKAMGLMI
                     NANKKSGMARIAKMMHKDAAWLEAHPQYKAYSWDFHFDETKHQDGFYYHFTQCPLNTF
                     ARKEGYLEVLPIMCDIDYKTAALMHAKLYRDHTLAGGGEVCDYWFVGDKVENPK"
     gene            975..1781
                     /locus_tag="PLN77_01555"
     CDS             975..1781
                     /locus_tag="PLN77_01555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM43000.1"
                     /translation="MSNEKIKPKLNGTAETMLQCFYARAMHSRNPKNKFRDTKAEELI
                     DRIDYDFSNAKKDTAMSGGIVARSVVFDELVSDFIRKNPDCTVVNIACGLDTRFYRMD
                     NGKITWYNLDLPETIEVRDSIYQESGRVSTIACSVLDPAWADQVKVRGKMLFIIEGLT
                     MYMKADEVRMMLAIIRDHFDNAYVCMETLCPYFVKKENIEKSIQATGATFTWGANSFA
                     DLGDIAAGFRKVKDDNIARGMETLNPIYKLVMWMPIVHKFAEKILVFEKA"
     gene            complement(1887..2543)
                     /locus_tag="PLN77_01560"
     CDS             complement(1887..2543)
                     /locus_tag="PLN77_01560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM43001.1"
                     /translation="MRVLITGTSSGIGKAIAEKFLKEGFDVTGFDRKEASISQDSYTH
                     FCLDIRDREQYPALAPFDIVINNAGVQNENDIDINLKGTIAITEAYGIHDTIRAVLMI
                     GSASGHTGAEFPEYTASKGGVLAYTKNVALRIAPYGGTCNSLDFGGVLTELNLPVTED
                     QKLWDQIMDLTPLKRWMTVEETADWAYFMTVTNRFCTGQNILIDGLEAGNAHFVWPDE
                     "
     gene            2773..3504
                     /locus_tag="PLN77_01565"
     CDS             2773..3504
                     /locus_tag="PLN77_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM43002.1"
                     /translation="MLKVDNLTFEVNEEGRKRCLVNHISFEVKDGEMLVITGPNGGGK
                     STLAKVLAGIEDANGGEIFLDEENITGYDIDHRANAGIGYAFQQPPRFKGMTVKKMLS
                     LAAGKELTEAESCKLLSAVGLCSADYLNRQMDGTLSGGEMKRLEIATVLAKPHKVSIF
                     DEPEAGIDLWSFSMLIRRFEEIHKEKKECLILISHQERIIQMADKIMVIEDGQVKEYG
                     TKEDVLPTLFQQKTGRMCGQEGGTC"
     gene            3504..4427
                     /locus_tag="PLN77_01570"
     CDS             3504..4427
                     /locus_tag="PLN77_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRM43003.1"
                     /translation="MVQVDETTQGVLNQISEGFEQKGAYNLRQNGIPLCHGDSEHIKI
                     RKKEDKPGIDILIDGETKGEKVFIPVVVSVSGMTDLVYNDFYVADGADVTIVAGCGIH
                     NGGCNDSRHDGIHTFHVGKNANVRYEEKHYGEGNGTGQRILNPVTNIEIGENSVFTLD
                     TVQIKGVDSTIRTTNVVMDANSKLFVLERLMTDGDQKAESDIEVCMNGSGSSAQIISR
                     SVGKGNSSQVFHPKAVGKNKCHAHIQCDSIIMDHAQVGSIPEIQAKHVDAAIIHEAAI
                     GRINDEQLLKLRTLGMTEAEAENVIIENFLS"
     gene            4614..5261
                     /locus_tag="PLN77_01575"
     CDS             4614..5261
                     /locus_tag="PLN77_01575"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HRM43004.1"
                     /translation="MGKAKMGIVINHSENIFLPQMIITKPEMEEKVEAFVKNGGTVIV
                     TYRHAVKDADNNVPFGETLPVHYNALAGLTVEETESLQDYDAFPVVGSGVFEGVEGTG
                     GIFRDMIQVQDAEVLFHYADAFYLEFAAVMRKQTGRGTLYYVSCGLEEKITKLLMEQV
                     MRDWHFQMVPSEEGLEIVTRGNEKQKVTMYINHNAKEVTYGDMTLAPFACKILEA"
     assembly_gap    5371..5380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5585..6421
                     /locus_tag="PLN77_01580"
     CDS             5585..6421
                     /locus_tag="PLN77_01580"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HRM43005.1"
                     /translation="MEKQKKIALINDLTGFGRCSTAVMAPIVSALPIQAVAVPTAILS
                     AHTQFPTYYFDDYTDRMKDYIQTYKDLKLDFDAISTGFLGSEQQVDIVLDFIRHFKTD
                     RNFVIVDPVMGDYGKLYRTYTKEMCEKMKELVHYADIITPNLTELCTLIDAPYPENGA
                     SIEELKEMCGKLAERGPKHIVVTGIHFNQTQIANFIYNKGEDFQIVMVDRIGGDRSGT
                     GDVIAAIIAGMYLNGHSLYESVKKAADYVSKCIRYCEENEVPSYWGLCFEMFMKDLTE
                     EA"
     gene            6421..7080
                     /locus_tag="PLN77_01585"
     CDS             6421..7080
                     /locus_tag="PLN77_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HRM43006.1"
                     /translation="MYLYTVTGAVGEEGYLDLAKSGDLTGKNVYVNMTNKCPCSCVFC
                     LRQTKQQQEGNTLWLKEGEPSVETMLELFAQYDLNVINELVFCGFGEPLERLEDVCRV
                     IDSLKETYPNLKVRLNTIGLANLIHGRDVTPELKGRFDTVSISLNAPDEKEFLELTRS
                     KFGIQSYEAIKEFAVLSKQYVPNVVMTVVEKVMPEEKIQKCQEICDTLGVTLRVRPFE
                     N"
     gene            7161..8543
                     /locus_tag="PLN77_01590"
     CDS             7161..8543
                     /locus_tag="PLN77_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HRM43007.1"
                     /translation="MNESNGVLRDARKLGIPKMLILGLQHMFAMFGATILVPILVNSY
                     FVDACGEGPSRGLTVSVTLFCAGFGTLLFHLCAKFKVPAFLGSSFAFLSGFAAVANLN
                     TGKYASMSVNDKAAYACGGIVVAGLLYLIFALIIRMVGVKRVMRYLPPVVTGPVIICI
                     GLSLAGSAINNASTNWFLALVALAVIIIFNIWGKGMFKIIPILMGVVIAYAVAVVLNA
                     LGIKNPDGSVIINFVPVAQAGIVGLPPLQLCKFDLTSIMIMAPIAIATMMEHVGDMSA
                     ISATVGENFLSDPGLHRTLLGDGLATAMAGFIGGPANTTYGENTGVLELSRVHDPRVI
                     RIAAVFAIIVSFIPKVSALISTMPSSIIGGVSFMLYGMISAIGVRNVVENKVDLTKSR
                     NLIIAGVIFVCGLGFSNGLSFTIGGTTVTLTALAIAAIAGILLNMILPGNDYEFGVNE
                     SGDENRGIHA"
     gene            complement(8826..9308)
                     /locus_tag="PLN77_01595"
     CDS             complement(8826..9308)
                     /locus_tag="PLN77_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRM43008.1"
                     /translation="MIRLAVPEDFTSIMSIYAYARSFMQETGNPNQWGNHFPPEELIH
                     NRIRDKQLFVLEENGTLHGAFAFIIGEDPTYLQIDDGSWLSDAPYGTIHQVASDGTIH
                     GFFSQIVDYCWAQIPHLRVDTHADNQIMQHLIQKNGFERRGIIYVLNHSPRIAFERVK
                     "
     gene            9525..10766
                     /locus_tag="PLN77_01600"
     CDS             9525..10766
                     /locus_tag="PLN77_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HRM43009.1"
                     /translation="MKQNRKRKETAGMNFLRYLGPGLLVTVGFIDPGNWASNIAAGSG
                     YGYELLWMVTLSTIMLIFLQHNAAHLGIVTGKCLSEAASLYLPAWLKNTILATAMMAA
                     VATAMAEILGGAIALQMLFGIPIKLGSMMILVLVLICEFTSAYKRIEKLIIVFVSLIG
                     FSFLFEVWIADIDWAQAAVGWVKPSFPEHSMPVILSVLGAVVMPHNLFLHSEIIQSRQ
                     WNLEDDEVIARQLKYEFKDTLFSMIIGWAINSAMILMAAATFFQPQNAKQVDDLATAG
                     KMLTPLIGNAASVIFAAALLLAGISSSITAGMAGGTIFSGIFNKPYEIKSKETRWGIL
                     LTMIPAAAVILLIDSPFQGLLYSQMFLGMQLPITVFTQIYLTSSKKVMGKYANSLRLK
                     IALFAIAVIVTLLNIALLFVQ"
     gene            complement(10843..11700)
                     /locus_tag="PLN77_01605"
     CDS             complement(10843..11700)
                     /locus_tag="PLN77_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM43010.1"
                     /translation="MELRQLEYFRVIAETESIHEAARRLNMSQPPLSYQLKQLEAELN
                     VTLFERTRQGVRLTEAGKLLYDRSANLLEYVKSTELEVAKVGKKRVLRLGITPTTVAT
                     IMPCVSLFSRRNPDVNFEVHDGITYTLYRYLMDGIIDISIARTPLRLDDVDYKILSSE
                     PMIAVSSKDMLPEKNGSLKLPDLLNIPLIIYRRYEALIMDAFHSRGMEPDLFCVCDDP
                     RGAMLWAKEGLATAIFPQSMSSLCQGLRIQPLAESDLETQIAVIWKKGRKLSPLLQDF
                     VDACMESIL"
     gene            11838..13598
                     /locus_tag="PLN77_01610"
     CDS             11838..13598
                     /locus_tag="PLN77_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43011.1"
                     /translation="MKSLGVYLKHYKKESIIAPLFKLLEVVFDLLVPMVVAQIIDVGI
                     AGNNRPYIVSRFGILVLMAAVGLCCSFTAQYFAAKASVGCATELRQAVFDHIQALSFT
                     ELDTIGTDTLITRLINDINQVQNGINMGLRLLLRSPFIVFGSVVMAFTVNVKCAIVFL
                     VTIPILFVAVFGIMLVSIPLFKKVQAALDRVTGMTRENLNGVRVIRAFCREEQSVADF
                     EKSNDELTCLNEFVGKISALLNPLTYVLINIAAVVLIWQAGLQVNIGSMQQGQVVALY
                     NYMLQIIVELIKLASLIITLNKSVACAERVSGVLAVPTTMNYPAKGGAAKKVTGDAAV
                     TFKNVSFTYAGAGAESLSDISFTAKRGQTIGIIGGTGSGKSTLVSLISRFYDATSGDV
                     MVDGENVKNYTKEELCNKVGVVQQRAVLFKGSIRDNMKWGKEDATDAEIWEALTTAQA
                     KEVVEGKQDQLDFMLEQNGKNLSGGQKQRLSIARALVKKPEILILDDSSSALDFATDA
                     KLRKAVHNLEGNTTTFLVSQRIAGIRQADQILVLGNGTLEGNGTHDELMKTCEVYREI
                     YFSQFPEERAAYNNESGVIA"
     gene            13595..15349
                     /locus_tag="PLN77_01615"
     CDS             13595..15349
                     /locus_tag="PLN77_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43012.1"
                     /translation="MKKTENKSGKSGMQVMKKLMAFIGNYKIFLGLSIVLAAVTVILQ
                     LYIPILFGDAIDEVVAAHQVNFPEMWFYLKRIVFFTLVSALSAWIMNLINNRMTYRIV
                     RDLRSQAIRHIQVLPLSYLDQHSSGDIVSRVIADTDILSDGLLLGFTQLFSGIVTIIV
                     TLFFMFSKNVWITLLVICLTPLSFFVAKFISGRSFKMFQKQSETRGKQTALIDEMIGN
                     QKVVKAFGYEEKASERFAEINKDLQKYSQQAVFYSSLTNPSTRFVNNVIYAGVALVGA
                     FMIPGGALTVGGLSVLLSYANQYMKPFNDISSVITEMQNALACAGRIFALLEEKEESA
                     DPAGMIDTVTGNVTIDDVAFSYDKEKKLIENFNLDVQPGMRVAIVGPTGCGKTTFINL
                     LMRFYDVDAGKICIDGKPIDELSRHALRSCFGMVLQDTWIKQGTVRDNISFGKPDATE
                     EEIVNAAKEAHSWEFIKRLPKGLDTVLSEDSISQGQKQLLCITRVMLCLPPMLILDEA
                     TSSIDTRTELQVQEAFDKLMQGRTSFVVAHRLSTIRNASLILVMKDGKIIEQGRHEEL
                     LEKKGFYYNLYNSQFQAS"
     regulatory      15661..15751
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            15961..17325
                     /locus_tag="PLN77_01620"
     CDS             15961..17325
                     /locus_tag="PLN77_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRM43013.1"
                     /translation="MDALNQAIAQISDVLWNSVLLFLLVGTGVYFSVRTRFVQVRKFK
                     TAWNRVFGGFSLKGKKAGKDGMTSFQALATAIAAQVGTGNIAGCATALVSGGPGAIFW
                     MWLAAFFGMATIYGEAYLAQISKRRDENGEVIGGPVYYITHAFKGTFGKALAGFFAVA
                     VILALGFMGNMVQSNSISDAFYTAFSVPKWVMGVIVAVLAAFIFIGGISRIASFTEKV
                     VPVMAALYLVGALVVILINIQHVPSAIASIFICAFRPDAVFGAAAGITVRKAMRYGVA
                     RGLFSNEAGMGSTPHAHAIADVENPEDQGEVAMIGVFIDTFVVLTMTALVILSSGVLE
                     EMMSTGVKGTPVAQAAFRTGLKGFGPAFVAICLLFFAFSTIVGWYFFARQNVQYLFGK
                     KAVVPFSLIVVVFVFLGSLLKVDLVWNLSDLFNGLMVIPNLMALIVLAGTVAKAAKGE
                     KVEF"
     gene            17878..18063
                     /locus_tag="PLN77_01625"
     CDS             17878..18063
                     /locus_tag="PLN77_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43014.1"
                     /translation="MTRNSKNEYSKNEYSKNGYEKSEYSKNQKNSRDCNHVKDREMSS
                     YGQQNREQDEKDEYEKY"
     gene            18183..18500
                     /locus_tag="PLN77_01630"
     CDS             18183..18500
                     /locus_tag="PLN77_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM43015.1"
                     /translation="MAGMTLLAADQLDWYVGKPWAMIIDVRNRREYEKGHVKGAVNLP
                     YDHLLERVCLPRDKILVICCERGAASMAEGKRLAENGYEVKTVIGGMHAYRGRYLVAG
                     EEL"
     gene            18607..19149
                     /gene="yfcE"
                     /locus_tag="PLN77_01635"
     CDS             18607..19149
                     /gene="yfcE"
                     /locus_tag="PLN77_01635"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HRM43016.1"
                     /translation="MKYMFASDIHGSAYYCRKMLEAFEAEGASRLFLLGDLLYHGPRN
                     DLPKEYAPKQVIAMLNEKKQYLTNVRGNCDAEVDQMVLEFPVLADYGILIEGGHTFYM
                     SHGHIYNEQNLPPLMDGDIFLYGHTHVLRAEKKGAHTFLNPGSVSLPKEGNIPTYAIL
                     EDGVFTIKGFDGTVVKQLTL"
     gene            19166..20323
                     /locus_tag="PLN77_01640"
     CDS             19166..20323
                     /locus_tag="PLN77_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRM43017.1"
                     /translation="MELYELIAPCHFGLEAVMKREILDLGYEIERVEDGKVTFLADAE
                     GIAYANLFLRTTERILLKVGQVHAETYDELFEATKALPWEKFIPKNGKFWVTKANSIK
                     SKLFSPSDIQSIMKKAIVERLKQVYHMEWFPEDGAQYPIRVSILKDEVTIGLDTSGVS
                     LHKRGYRKLTAKAPITETLAAALIMLTPWKGDRILVDPFCGSGTFPIEAAMMAADIAP
                     GLNRSFLAEQWTHLVPKKCWMDAAEDARYRIRNDIETDIQGFDIDGDVIKSARENAKL
                     AGVDHLIHFQQRPVAELSHPKKYGFLITNPPYGERLEDKKDLPALYQTLGERYRALDS
                     WSMYLITSYEDAERYLGKKADKNRKIYNGMLKTYFYQYAGPKPPRREKEQK"
     gene            20485..21009
                     /locus_tag="PLN77_01645"
     CDS             20485..21009
                     /locus_tag="PLN77_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43018.1"
                     /translation="MNGQEIRDRLLHSYERSYDIVKPSEVNGHTYDACASYHESGAKY
                     VLSKKAELWRISCHEHAYFKAVEELNDQDVETFLTDLTEWIEPKVVREGKEVPDTDHM
                     YTLVTGIFFADKPVADSVKKKIRKYKYYKNYRFSLRGYCQVRLLVFDTAEQKVFGNRA
                     AWELVKGYAKMFRS"
     gene            21054..22784
                     /locus_tag="PLN77_01650"
     CDS             21054..22784
                     /locus_tag="PLN77_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HRM43019.1"
                     /translation="MNSILVLVVGIGILIAGYVFYGKWLAEQWGVDPKRKTPAFTEED
                     GVDYVPAKAPVLMGHHFSSIAGAGPINGPIQAAVFGWVPVLLWVLIGGIFFGGVHDYG
                     ALFASLRHKGQSIGEVIADTMGSKAKKLFIVFAYLTLILVVAAFGSIVANTFKAAYTE
                     SGAVDVAASSANATTAMISILFIVLAVVFGFMVYRRNVSLGISTIAGVAAIVVCVVVG
                     LNFHPIYLSETAWMVIVGIYITVASVAPVWILLQPRDYLSSFLLYFMMIVAAVGVIGS
                     ALMGHASLDIPAFTGFKDTLAPTGSSLGFMFPALFVTIACGAISGFHSLVGSGTTSKQ
                     LDNEKNARPIAYGGMLIECALAIVSLCAVGYIWSRYADGTTVVPTAVFATGISEMVAT
                     IPGLGGSTHVLYSLLVLTVSVFCLTSLDTATRLARYMFQEFWLEPGQTYKEATGYKAV
                     LTNPVVSTVITVVLGIGLGLTGYSKIWPLFGASNQLLAAIGLLAVATWLGKVGKNNKM
                     FLFPMVFMLIVTITSLVQTLIANFKGLGAGVGIGWCVARIVLAGLLVILALILAVDGF
                     KTLANQKKAK"
     gene            22913..23515
                     /locus_tag="PLN77_01655"
     CDS             22913..23515
                     /locus_tag="PLN77_01655"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HRM43020.1"
                     /translation="MKKIVFATGNAGKMKEIREILADLPVEIYSMKEAGISVEIDENG
                     KTFEENAKIKAQTVAGCTDGDTIVLADDSGLEVDALNGEPGVYSARYMGEDTSYRIKN
                     QSLVDRLEGVPVEKRTARFVCVIAAAFPDGTVCTTKGTIEGKIGYEERGENGFGYDPI
                     FYLPDMSRTTAELSPEEKNAVSHRGKALAAMKEQIASYLK"
     gene            23536..24015
                     /locus_tag="PLN77_01660"
     CDS             23536..24015
                     /locus_tag="PLN77_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM43021.1"
                     /translation="MKFLIVSDTHGRMYNLEEAMEREEFDGMIHCGDVEGMDDLIKSK
                     VNGPCYMVMGNNDWFSNLPAEIQVNIDDYRAFITHGHNYGVSLGLEGIYAEALSRGVD
                     IVLFGHTHRPVAEKRGNLWIVNPGSLTYPRQMGRKPSYAMLTINEEHELSVEIKYLD"
BASE COUNT         6650 a         5636 c         6491 g         5603 t
ORIGIN      
        1 ggcgaaaata ttaaatagct ataagaagtg gccatccatg cttaccagag agcaggatgg
       61 ccatttctta atttctataa taaaggaagg ctgaattata gtcaagaaat ttttgcgatt
      121 ttaaaacaaa gtaataaaaa gtgtgatata ataacctcat aatgaaaagt aaaattccgc
      181 ctatttttca acattgtagg cggcaatttt acacctataa gttagtgaat cctaataaaa
      241 gtaagatgga ggtacccgcc catgatggaa tacaccagca aatattggaa catgctgact
      301 cctttaataa agaaaagtct gctaaaacgc tacggaaaag aggagaccgc agagcttctc
      361 cgcaagacca accagatcta ccgcgacatg cttcgccgcg ccgatgatat cggaaaggac
      421 aacccgatgg cgtcgaatct ctatgaaggc ctcatttttc ttgcgctctg ggatgcgtcg
      481 gacagacaga tttctgtgga tgaactgcgt acaatctcag aggagatgat ggccttcccg
      541 cttctgaaag ccatgggcct gatgattaat gcgaataaga agagcggcat ggcgcggatt
      601 gcgaagatga tgcataagga tgccgcctgg ctggaagcac atccgcagta taaggcgtat
      661 tcgtgggatt ttcattttga tgagacgaag catcaggacg gtttttatta ccacttcacc
      721 caatgtcccc tgaacacctt cgcccgaaaa gagggctatc tggaagtgct gccgattatg
      781 tgcgacattg attacaagac agcagccctg atgcacgcga agctctaccg tgaccatacg
      841 ctggcaggcg gcggtgaggt ctgtgattac tggttcgtgg gggacaaggt ggaaaatccg
      901 aaataaaaca agaccggacc ggaaccggtg aaatggatcg aagaatataa aacgcagaag
      961 ggagatcacc cagaatgagc aatgaaaaaa taaaaccaaa actgaacgga accgccgaga
     1021 cgatgctgca gtgcttctac gcccgcgcca tgcacagcag aaatccgaaa aacaagttcc
     1081 gcgacaccaa agcagaagag ctgattgacc gcatcgacta tgatttcagc aatgcgaaaa
     1141 aggacacagc aatgagcggc ggaatcgtag cgcggagtgt tgtgtttgat gagctggtca
     1201 gcgactttat ccgaaagaac ccggattgta ccgtcgtcaa catcgcctgt ggtctggaca
     1261 cacggtttta ccgcatggat aatggaaaaa tcacctggta taatctcgat ctgccggaga
     1321 cgattgaggt acgcgattcg atctatcagg aatccggccg cgtttccacg attgcgtgtt
     1381 ccgtgctcga tccggcatgg gccgatcagg taaaagtgcg gggcaaaatg cttttcatta
     1441 tcgaaggtct gaccatgtac atgaaagcgg atgaggtcag aatgatgctc gccatcatcc
     1501 gcgatcattt cgacaatgcc tacgtctgca tggaaacgct gtgcccgtac tttgtaaaga
     1561 aagaaaacat cgaaaaatct attcaggcga cgggcgcgac gtttacctgg ggtgcgaaca
     1621 gctttgcaga cctgggcgac attgccgccg gtttccgaaa agtaaaagac gacaacatcg
     1681 cccgcggtat ggaaacactg aatcccatct acaagctggt gatgtggatg ccgatcgtcc
     1741 acaaattcgc agagaaaatt ctggtgtttg agaaagcgta agaaaaaacg aaaaaaatag
     1801 aaagaaacag gcagcccggc agcggaaaag ctcaaaaagc ttccgtgcag ggaacctgtt
     1861 ttttgtttgt gcaggaaatt aacaaatcat tcatccggcc acacaaagtg tgcgtttccc
     1921 gcttcaaggc cgtcaatcag aatattctgc cccgtgcaga accggttcgt cacggtcata
     1981 aaatacgccc agtccgccgt ttcctcgacg gtcatccacc gtttcagcgg cgtcagatcc
     2041 atgatctgat cccagagctt ttggtcttct gtgacaggca gattcaactc ggtcagtacg
     2101 ccgccgaaat caaggctgtt gcacgttccg ccatacggtg caatgcggag tgccacgttt
     2161 ttcgtgtagg cgagcacacc gcccttgctt gccgtgtatt ccggaaactc agctccggta
     2221 tggccgctgg ccgagccgat cataaggacg gcgcggatgg tgtcatgaat gccatacgcc
     2281 tctgtgattg cgatggttcc ctttaaattg atatcaatat cattctcgtt ctgcacaccg
     2341 gcgttgttga tgacaatatc gaaaggcgca agcgccggat actgttccct gtcgcggata
     2401 tcaaggcaga aatgagtata cgaatcctgg ctgattgaag cctccttccg gtcaaatccg
     2461 gtaacgtcaa agccttcttt taaaaatttc tccgcaatcg ccttcccgat tccggaactg
     2521 gttcctgtaa taagtacacg catacgataa cctcccgatg aaatattacc ctcattatac
     2581 cgcaaacaca gcatgcgatc cagaagaaac gaaaaagaat gcaggaaaag aaaatgttac
     2641 gggaaaaatc actgcataaa acaaaagagt ctcccaaact gcaacaaata attgacagat
     2701 tttaggagaa ggagtagagt ttttgaaagg gggattatcc ctgcaaagag tgaaaacaaa
     2761 agaggtgcaa atatgctgaa agtagacaat cttacctttg aagtaaacga agagggaaga
     2821 aaacgctgcc tcgtcaacca catcagtttt gaggtaaagg acggggaaat gctggtgatt
     2881 accggaccga acggtggcgg aaaatcaacg ctggccaaag tgcttgcagg aattgaggac
     2941 gccaatggcg gagaaatctt cctggatgag gaaaatatta ccggttatga tattgaccac
     3001 cgtgcaaacg ccggcatcgg ctatgcattc cagcagccgc cgcgttttaa aggaatgacg
     3061 gtgaaaaaaa tgctgtcgct ggcagccgga aaagaactga cggaagccga gagctgtaaa
     3121 ctcctctccg ccgtcggact ctgctctgcc gattacctga accgccagat ggacggtacg
     3181 ctctcgggag gagagatgaa gcgtctggag atcgcaacgg tgcttgcaaa accgcataag
     3241 gtcagcattt tcgatgagcc ggaggccggc attgacctgt ggagcttttc catgctgatc
     3301 aggcggtttg aagaaatcca caaagagaaa aaagaatgtc tgattctcat ttcccatcag
     3361 gaacgcatta tccagatggc agacaagatc atggtgatcg aggacggcca ggtgaaagaa
     3421 tacggtacaa aggaagacgt actgccgacc ctgttccagc agaaaacagg ccggatgtgc
     3481 ggccaggaag gaggcacatg ctgatggtac aggtagatga gacaacccag ggcgtattaa
     3541 atcagatcag cgagggattt gagcagaaag gggcatacaa tctccgccag aacggcattc
     3601 cgctctgcca cggtgacagt gaacatatca aaatccgcaa aaaagaggat aagccgggca
     3661 ttgatatttt gatcgacggc gagacgaagg gcgaaaaagt atttatcccg gttgtcgtca
     3721 gcgtttccgg catgacggat cttgtatata atgatttcta cgttgcggac ggcgccgatg
     3781 ttacgattgt agcgggctgc ggtattcaca acggcggctg caacgacagc cggcacgacg
     3841 gtatccatac gttccatgta ggaaaaaatg ccaacgtgcg ctacgaggaa aagcattacg
     3901 gagagggaaa cggaacggga cagcggattc tgaacccggt gacaaatatt gaaatcggag
     3961 aaaattccgt ttttacgctg gataccgtac agattaaggg cgttgactca acaattcgta
     4021 cgacgaatgt tgtgatggat gcaaactcca aactgttcgt gctcgaacgt ctgatgacag
     4081 acggtgacca gaaggcagag tcggatattg aagtatgtat gaacggaagc ggttcttccg
     4141 cacagattat ttcacgttct gtcggaaagg gcaattcctc ccaggtcttc catcccaagg
     4201 cagtcggcaa aaacaaatgc cacgcccata tccagtgcga ttctatcatc atggatcacg
     4261 cacaggtcgg atctattccg gagatccagg caaaacatgt ggatgcggca atcatccacg
     4321 aggcggcgat cggacggatc aatgatgagc agctattgaa actcagaacg ctcggcatga
     4381 cggaagcgga ggcggaaaac gtcattattg aaaacttcct gagctgaaga aaaaaaggaa
     4441 acaagagaac aaaaaagagg catttccagt ctgcatagat ggagatgcct ctttttgtgc
     4501 atttttcaca aaagcaggta gtaaatttta ctaaaataaa aagtaaaata ttttgacttt
     4561 taacatatga taaatttgta ctgttgaaaa aaggaaagat acaagataga gaaatgggga
     4621 aagcgaaaat gggtattgtg atcaatcatt ccgaaaatat tttcctgccg cagatgatca
     4681 ttacgaagcc ggagatggaa gaaaaggtgg aagcgtttgt gaaaaacggc ggaaccgtaa
     4741 tcgtgacgta ccgacacgcc gtgaaagatg cggacaacaa cgttccgttc ggcgagacac
     4801 tgccggtgca ctacaatgcg cttgcgggtc tgaccgtaga agagacggag agcctgcagg
     4861 actatgatgc attcccggtt gtgggaagcg gcgtgttcga gggcgtggaa ggcacaggcg
     4921 gaattttccg tgatatgatt caggtgcagg atgcggaagt gctgttccat tacgcggatg
     4981 cgttctatct ggaattcgca gccgtgatga gaaaacagac cggcagggga actttgtatt
     5041 acgtgagctg cggtctggaa gagaagatca cgaagcttct gatggagcag gtgatgcggg
     5101 actggcattt ccagatggtg ccgtccgaag agggccttga gatcgtgacg agaggaaacg
     5161 agaagcagaa ggtgacgatg tacatcaacc acaatgcaaa ggaagtgacg tatggggata
     5221 tgacactggc accgtttgca tgcaagattt tagaagcata atataaaaga gtaaacgaga
     5281 gaaaaggcgc ggccgcccgg agcaaaaaac agcttcgggc gggccgtgcc ttttgcgtgc
     5341 tgacaaaaaa gatgcaaaaa gcggaattct nnnnnnnnnn gacggaaaaa ctttaaaggg
     5401 agagcagaaa tgaatcagaa aaaagacaaa attcagttat ccgatcattt tacatacggg
     5461 gcggtctatt tcccgacagc cccttttctt tttgtccgga aaaaaacttg taaataagac
     5521 gcgagtgtgc aaaaatagac ataaaacaga tgcggaggca tcctgaaaaa aagaggagaa
     5581 caacatggaa aaacagaaaa agatagcact gattaacgat ctgacaggct ttggccggtg
     5641 ctcgacggct gtgatggcgc cgatcgtgtc ggcgctgccg attcaggcgg tggcagttcc
     5701 gaccgcaatt ctctcagcac acacgcagtt tccgacgtat tactttgatg attatactga
     5761 ccggatgaag gattacattc agacctataa ggatctgaag ctcgatttcg atgcgatctc
     5821 gaccggcttc ttaggatcgg aacagcaggt ggatatcgtg cttgatttta tccgccattt
     5881 taagacggac cgcaactttg tcatcgtcga tccggtcatg ggggactatg ggaagctgta
     5941 tcggacgtac acgaaagaga tgtgcgaaaa gatgaaagaa ctggttcact acgcggatat
     6001 catcacgccg aacctgacag agctctgcac actgatcgat gcgccgtatc cggagaacgg
     6061 ggcttcgatt gaggagctga aagagatgtg cggaaaactg gcggagcggg gaccgaaaca
     6121 catcgtggtg acgggcatcc actttaacca gacacagatt gccaacttta tctataacaa
     6181 aggcgaggat ttccagatcg tcatggtaga ccgcatcggc ggcgaccgga gcggtacggg
     6241 agatgtcatt gcggcaatta tcgcaggcat gtatctgaac ggccacagcc tgtacgagtc
     6301 ggtgaaaaag gcggcggatt acgtatcgaa atgcatccgc tactgcgaag aaaacgaagt
     6361 gccgagctac tggggactct gctttgaaat gtttatgaag gacttaacgg aggaagcgta
     6421 atgtatttat atactgtaac cggagccgtc ggcgaggaag ggtatctcga ccttgcaaag
     6481 agcggcgatc ttaccggaaa aaatgtctat gtcaacatga caaacaaatg cccgtgcagc
     6541 tgtgtgttct gtctgcgcca gacgaaacag cagcaggagg gaaatacact gtggttaaaa
     6601 gagggcgagc catccgttga gacgatgctg gagctgttcg cacagtacga tctgaacgtg
     6661 atcaatgaac tggtcttctg cggcttcgga gaaccgcttg agcggcttga ggatgtctgc
     6721 cgggtgatcg attccttaaa agaaacgtat ccgaacctga aagtgcgtct gaatacgatc
     6781 ggtcttgcca acctgatcca tggaagagat gtcaccccgg aattaaaggg aagattcgat
     6841 accgtctcga tttccttaaa cgcgccggat gaaaaggaat ttctggagct cacgcggtcg
     6901 aaattcggca tccagtcgta cgaggcgatc aaggagtttg cggttctttc caaacagtat
     6961 gtgccgaatg ttgtgatgac ggttgtcgag aaagtcatgc cagaggagaa aattcagaag
     7021 tgccaggaga tctgcgatac cttaggagtt actttacgtg tccgcccgtt tgaaaattga
     7081 gaggcccgca atctttcgct tgtatttgaa cgacataacg cctataatgg gaaggaaaaa
     7141 caaaagagag ggagagacgt atgaacgagt ctaatggagt cctccgcgat gcgagaaagc
     7201 tcggcattcc taaaatgctg attctcggat tgcagcacat gtttgccatg ttcggtgcaa
     7261 ccattctggt gccgattctg gtaaacagct actttgtaga cgcatgcggc gaggggccgt
     7321 cccgcggtct taccgtatcc gtcacactgt tctgcgccgg ctttggaacc ctgctgttcc
     7381 atctgtgcgc gaaatttaaa gtaccggcat ttcttggttc ttcgtttgca ttcttaagcg
     7441 gtttcgccgc tgttgcaaac ctgaataccg gaaaatatgc cagcatgagt gtcaacgaca
     7501 aggcagccta tgcctgcgga ggtattgtgg tagcaggtct tctgtacctg atttttgccc
     7561 tgatcatccg catggtcggc gtcaaacgcg tgatgcgcta cctgccgccg gttgtcacag
     7621 gtccggttat catctgcatt ggcctcagcc ttgctggttc cgccattaat aatgcttcga
     7681 cgaactggtt cctggcactg gttgcgctgg cagtcatcat cattttcaac atctggggaa
     7741 aaggaatgtt caaaatcatt ccgattctga tgggcgttgt gattgcctac gctgtggcag
     7801 ttgtcctgaa tgcactcggc atcaaaaatc cggacggatc cgtgatcatc aactttgtgc
     7861 cggttgcaca ggcaggcatc gtcggacttc cgccgctgca gctctgcaaa ttcgatctga
     7921 ccagcattat gatcatggca ccgatcgcga tcgcgaccat gatggagcat gtcggcgata
     7981 tgtcggcgat ctctgcaacc gtaggagaga acttcctttc tgatccgggc ctgcacagaa
     8041 ccctgctcgg cgatggtctt gcaaccgcaa tggcaggttt catcggcgga ccggccaaca
     8101 cgacgtacgg cgagaacacc ggcgttctgg aattgagccg cgtacatgac ccgagagtca
     8161 tccgtattgc cgcagtcttt gcgattatcg tcagcttcat tccgaaggta tcggcactca
     8221 tcagcaccat gccgtcctct atcatcggag gcgtcagctt catgctgtac ggtatgattt
     8281 ctgccatcgg tgtgcgcaac gtggtagaga ataaagtaga cctgaccaaa tcgagaaacc
     8341 tgatcatcgc gggcgtcatc tttgtctgcg gactgggctt cagcaacggt ctctccttta
     8401 cgatcggcgg aacgaccgtc accctgactg cacttgccat tgcagccatc gcaggaatcc
     8461 tgctgaacat gattctgccg ggcaatgatt acgaattcgg cgtaaacgag tccggggacg
     8521 agaacagagg aatccacgca taaagaaaaa tgaaaaagaa aatccgtttt ttctgcatct
     8581 gtattctgca caaaggaatg tgcagaaaca gaggggaaaa agcggatttt ctttgcattt
     8641 cggtacgggc aagtgcatac tggagagtaa gaagatgtaa ggctggtaaa aaaatgacac
     8701 acgaaagatc gaaaaatggg atgatgggac aggtcgtcgg atccttatat tatctggatc
     8761 caacggtgat tccgcggctg aaaagtctgc gaagcggtga cgatccggtg ttttcgtttg
     8821 gtgttttatt ttacgcgctc aaacgcaatc cgcggagagt ggttcagcac gtagatgatg
     8881 ccgcgccgtt caaatccgtt tttctggatc agatgctgca tgatttgatt gtccgcatgc
     8941 gtatcgacgc gcaggtgcgg gatctgtgcc cagcagtaat cgacgatctg ggagaaaaat
     9001 ccgtggattg ttccgtctga ggcaacctgg tggatcgtgc catacggcgc atcggagagc
     9061 caggagccgt cgtcgatctg cagatacgtc ggatcctcac cgatgatgaa ggcaaacgcg
     9121 ccgtgcagcg tgccgttttc ttccaggaca aaaagctgtt tgtcacggat tctgttgtga
     9181 atcagctcct ccggcggaaa atggtttccc cactgattcg gatttccggt ttcctgcata
     9241 aaagagcggg cgtacgcata gattgacatg atcgaagtaa aatcctccgg gacggcaagt
     9301 cgtatcatga taaaaattct ccttttagtt caaaataagc agttttattg tagcacagcg
     9361 gacgatgaag cacaagaaag agaaaacagg atttgacttt ttgaaaaatc ccatgtacaa
     9421 tgcagttagt tacaactaac taaagtaacc aaaaccacaa caaaacgatt tctgccggtc
     9481 ctggatgggc cggttccgta tcaaattgtc agaaaggaaa gaatatgaaa caaaatcgaa
     9541 aacgaaaaga gacggcgggc atgaattttc tccgctatct cggcccggga cttcttgtca
     9601 cggtcggttt tatcgacccg ggaaactggg catccaacat tgccgccgga tcgggttacg
     9661 gctacgagct tctgtggatg gtcaccctgt ccacgatcat gctgattttt ctgcagcaca
     9721 atgcggcgca tctcggcatt gtcacgggaa agtgcctctc ggaggcggcg agcctctatc
     9781 tgcctgcatg gctgaaaaac acgattcttg cgacggcgat gatggcagca gtggcgaccg
     9841 caatggcgga gatcctaggc ggtgcgattg cgctccagat gctgttcggc attccgatca
     9901 agctcggcag catgatgatt ctcgtgctgg ttctgatctg tgagtttaca agtgcttaca
     9961 aacggattga aaaactgatc attgtgtttg tctcgctgat cggcttttcg ttcctctttg
    10021 aggtctggat cgccgatatc gactgggcgc aggcggctgt cggctgggta aaaccgtcgt
    10081 ttccagagca ctcaatgccg gtcatcttaa gcgtcctcgg tgcggtcgtc atgccgcaca
    10141 acctgtttct ccactcggaa attattcaga gccgccagtg gaatcttgag gatgatgagg
    10201 tgattgcgcg ccagttaaag tatgagttca aagacaccct gttttccatg atcatcggat
    10261 gggccatcaa cagtgcaatg atcctgatgg cggccgcaac gtttttccag ccgcagaatg
    10321 caaagcaggt ggatgatctc gcaacggcgg gaaagatgct gacaccgctc attggaaatg
    10381 ccgcatcggt gatttttgca gcagcgcttc tgctcgccgg aatttcatcg agcatcaccg
    10441 cgggaatggc gggcggaacg attttttcgg ggatttttaa caagccgtat gagatcaaga
    10501 gtaaggagac acgctgggga atccttctta ccatgatccc ggccgcagcg gtcattcttc
    10561 tgattgattc cccgttccag ggccttctct attcacagat gttccttgga atgcagctcc
    10621 cgattacggt atttacgcag atttatctga cgtcctcgaa aaaagtcatg ggaaaatacg
    10681 cgaacagtct gcggctgaaa attgccctgt ttgcgattgc ggtgattgtg acactgctga
    10741 atatcgcgct gttgtttgtg cagtaaaaag aatgagaaat aaaaaaagga agtccgctgc
    10801 gctgatccct gaaaaagaga ccggcgcagc ggacttcttc tattataata tgctctccat
    10861 gcaggcatcg acaaagtcct gcagaagcgg agagagtttc cgcccctttt tccagatcac
    10921 ggcgatctgc gtttccaggt cgctttctgc gagcggctgg atgcggagtc cctggcagag
    10981 agagctcatg gactgcggga aaatggcagt ggcaaggcct tcctttgccc agagcatcgc
    11041 gccgcgggga tcgtcgcaga cgcagaaaag atcgggctcc atgccgcgcg agtggaacgc
    11101 atccatgatg agcgcctcat accggcggta gatgatgagc ggaatattca aaagatccgg
    11161 gagctttagc gatccgttct tctcgggaag catatctttt gaagaaacgg caatcatagg
    11221 ttcagagctt aaaattttat agtcgacatc gtcgagccgc agcggcgtgc gggcgatgga
    11281 aatgtcgatg atgccgtcca tcagatagcg gtacagcgtg taggtgatgc cgtcgtgcac
    11341 ctcgaagttg acatcgggat tgcggcggga aaaaagcgag acgcacggca tgatcgtcgc
    11401 aacggtggtc ggtgtgatgc cgaggcgcag aacccgcttt ttgccgacct ttgcgacctc
    11461 aagctccgtc gatttcacgt actcgagcag gttggcggag cggtcgtaca gcagttttcc
    11521 ggcttcggtc aggcggacgc cctggcgggt gcgttcaaat aaagtgacat tcagttcggc
    11581 ttccagctgc tttaactgat aactgagcgg cggctgggac atattaaggc ggcgggcggc
    11641 ctcgtgaatg ctttcggttt cggcgatgac gcggaaatat tcaagctggc gcagttccat
    11701 aagagacttc ctttcgtgaa attatctatg atattaaaat tgtatcacta agctataaaa
    11761 acaatactat tcgtattaat catatgagat tatactgtca atgtcaagaa acaaagaaat
    11821 ggagagtgaa aaagaagatg aaaagtcttg gagtctactt gaagcactac aaaaaagaga
    11881 gcatcatcgc tcctttgttc aagcttctgg aagtcgtatt cgaccttctg gtgccgatgg
    11941 ttgttgccca gatcatcgat gtgggtatcg caggcaataa ccgtccgtac atcgtcagcc
    12001 gttttggaat cctcgttctg atggcggcag tcggactttg ctgcagcttc accgcgcagt
    12061 atttcgcggc aaaagcgagc gtcggctgtg caaccgagct tcgccaggcg gtctttgacc
    12121 acatccaggc tctgtccttt acggagctgg ataccattgg aaccgatacg ctgatcaccc
    12181 gtctgatcaa cgatatcaac caggtgcaga acggaatcaa catgggtctg cgtctgctgc
    12241 tgcggagtcc gtttatcgta ttcggttccg ttgtgatggc atttaccgtt aacgtaaaat
    12301 gtgccatcgt attcctggtt actattccga tcctgttcgt tgccgtattc ggcattatgc
    12361 tggtcagcat cccgctgttc aaaaaggtac aggcggcact cgaccgcgtc accggaatga
    12421 caagagaaaa tctgaacggt gtccgtgtca tccgtgcgtt ctgccgtgag gagcagtctg
    12481 ttgccgattt cgaaaagagc aacgatgagc tgacttgtct gaacgagttt gtcggaaaaa
    12541 tttccgcact gttaaatccg ctgacctatg tcctcatcaa catcgcagcc gttgtcctga
    12601 tctggcaggc aggccttcag gtcaacatcg gcagtatgca gcagggacag gttgtcgcac
    12661 tttacaacta catgctgcag atcatcgtgg agctgatcaa actggcatca ctcatcatca
    12721 ccttaaacaa atccgttgcc tgtgcagagc gtgtctccgg cgtactggcg gttccgacaa
    12781 ccatgaacta cccggcaaaa ggcggcgctg caaagaaagt aactggcgat gcagctgtta
    12841 catttaaaaa tgtcagcttc acctatgcag gagccggtgc agagagctta tcggatatca
    12901 gttttaccgc aaaacgcggc cagaccatcg gaatcatcgg cggtaccgga agcggaaaat
    12961 cgacgctggt cagcctgatt tcccgtttct acgatgcgac cagcggtgac gtcatggtag
    13021 acggcgagaa tgtaaagaat tacacgaaag aagagctctg caataaagtc ggtgttgttc
    13081 agcagagagc agtgctcttt aaaggcagca tccgcgacaa tatgaagtgg ggcaaagagg
    13141 acgccaccga tgcagaaatc tgggaggcgc tcaccacagc tcaggcgaaa gaagtcgtag
    13201 aaggcaaaca ggatcagctc gattttatgc tggagcagaa cggaaagaac ctctccggcg
    13261 gacagaaaca gcgtctttct attgcgcgtg cgctggtaaa aaaaccggaa atcctgattc
    13321 tggacgacag ctcgagtgcg cttgactttg caacggatgc aaaactccgc aaagccgtcc
    13381 acaatctcga aggaaacacg acaaccttcc tcgtatccca gcgaatcgcc ggcatccgtc
    13441 aggcagacca gatcctcgtt cttggaaatg gtacgctcga aggaaacggc acacacgacg
    13501 agctgatgaa aacctgcgag gtttaccgcg agatttactt ctcccagttc ccggaagagc
    13561 gtgcagctta taacaacgaa agcggggtga tcgcatgaaa aagacagaaa acaagtccgg
    13621 aaaaagcggc atgcaggtca tgaaaaagct gatggcgttt atcggaaatt ataaaatttt
    13681 cttaggcctt agtattgtcc tggctgcggt taccgttatt ttacagcttt atattccgat
    13741 cctgttcggc gatgcgatcg atgaggtcgt tgcagcccac caggtcaatt tcccggagat
    13801 gtggttctat ctgaaacgaa tcgtattctt taccctcgtt tccgcacttt ccgcatggat
    13861 catgaacctg atcaacaacc gtatgacgta ccgcatcgtg cgcgaccttc gttcccaggc
    13921 gatccgtcat atccaggtgc tgccgctgtc ctatctggat cagcacagct ccggagacat
    13981 cgtaagccgt gttatcgcag ataccgatat cttatcggac ggtcttctgc ttggctttac
    14041 gcagctgttc tccggcatcg taaccattat tgtgacactg tttttcatgt tctcgaagaa
    14101 cgtatggatc acccttcttg tgatctgcct gacacctctc agcttcttcg tggcaaaatt
    14161 tatttccggc cgctcgttca aaatgttcca gaaacagagt gagacgagag gaaaacagac
    14221 cgcactcatc gatgagatga tcggaaacca gaaagtagtc aaagcgttcg gctatgagga
    14281 aaaagcttcc gagcgttttg cagagatcaa caaagatctg cagaaataca gccagcaggc
    14341 cgtgttctac agttccttaa cgaacccgtc cacccgtttt gtcaacaacg tgatctatgc
    14401 cggtgttgcc cttgtgggag ctttcatgat cccgggcggt gcgctgactg tcggcggact
    14461 ttccgttctg ctgtcgtacg caaaccagta tatgaaaccg ttcaatgata tcagttccgt
    14521 tatcaccgag atgcagaacg cgcttgcctg cgcaggccgt atctttgctc ttctggaaga
    14581 gaaagaggag agtgcagacc cggccggaat gatcgatacc gtgacgggaa atgtgacgat
    14641 cgatgatgta gcatttagtt atgacaaaga gaaaaaactg atcgagaact ttaatcttga
    14701 cgtacagccg ggcatgcgtg ttgccattgt cggcccgacc ggatgcggaa agacgacgtt
    14761 tatcaacctg ctgatgcgct tctatgatgt cgatgccgga aagatctgca tcgacggaaa
    14821 gccgatcgac gaactgtccc gccatgcgct ccgcagctgc tttggtatgg tgctgcagga
    14881 tacctggatc aaacagggaa ccgtgcgcga caacatcagc ttcggaaagc cggatgcgac
    14941 ggaagaggag attgtcaatg cggccaaaga ggcacacagc tgggagttta tcaagcgtct
    15001 gccgaagggg cttgacacgg ttctcagcga agacagcatc agccaggggc agaaacagct
    15061 tctctgtatc acccgtgtca tgctctgcct gccgccgatg ctgatcctcg acgaggcgac
    15121 ctccagcatc gatacccgta ccgaactgca ggttcaggag gcgtttgaca agctgatgca
    15181 gggcagaacg agtttcgtcg ttgcccaccg tctgtccacg atccgcaacg cttcgctgat
    15241 cctcgtcatg aaagacggaa agattatcga gcagggacgc catgaggagc ttcttgagaa
    15301 gaaaggcttc tactacaatc tgtacaatag ccagttccag gcgtcataaa agatatttgg
    15361 aaaagagaac agcagatctg tgccgcgcat gcgattttga gacgtgcgcg cgcagatctg
    15421 ctgatttttt atatattttt gcaatattcc gaaaaaaata cggcatgggg tattgacgaa
    15481 tcctatcaca ggtgctatag ttttacaaaa ataaaaaaag acgtagaagt ttttagaaaa
    15541 tgtgcggaga gcctttggaa atttaatttt cgaaaggtgc acgaccgaag aagtaaaact
    15601 ttcaggtgct ggttctcttc ttaaaacaag aatggacggg agacggcgag gactaaaaac
    15661 ggagtggtct ctggagaagc tcttgaaaag agtgccgaag gtgcaatgca gcgatgcaga
    15721 atctctcagg caaaaggaca gagtcaaaaa agatacagaa gaaaaacagc cgcggaacaa
    15781 aaaaagcggt gcgcagaaac gaaaaaattc cggaagtgcg gttttttgct gtatttttca
    15841 atcagagcca tgttctgcag gaaaaattcc gcaggagatg gtattttttt tctcaaaacg
    15901 gtgcggatgt gtgatccaca tcgttcagac catacgtcga atggaaaggg agaaaagaaa
    15961 atggatgcat tgaatcaggc aattgcgcag attagtgatg tgctctggaa cagcgtactg
    16021 ctgtttctgc tggtaggtac gggagtttat ttctctgttc ggacccgctt tgtacaggta
    16081 agaaagttta agaccgcgtg gaaccgcgtc ttcggcggtt tctcgttaaa gggtaagaag
    16141 gccggaaaag acggcatgac ttccttccag gcacttgcaa cagcgatcgc cgcacaggtc
    16201 ggaaccggaa acatcgcagg atgtgcgacc gcactcgtat ccggcggccc gggagccatc
    16261 ttctggatgt ggcttgctgc gttcttcggc atggcaacga tctacggcga ggcgtatctc
    16321 gcgcagatct ccaaacgccg cgatgagaac ggagaagtca tcggaggacc ggtttactac
    16381 attacccacg cattcaaggg aacgtttgga aaagcactgg caggcttctt tgccgtagcc
    16441 gttattctgg cgcttggctt tatgggaaat atggtacagt cgaactccat cagtgatgcg
    16501 ttctacaccg cattttccgt accgaaatgg gtgatgggtg tgattgtagc cgttctcgcc
    16561 gcattcattt tcatcggagg tatctcccgt atcgcatcct tcaccgaaaa agttgttccg
    16621 gtgatggcag cactgtatct cgtcggagcc cttgttgtta ttctgatcaa catccagcat
    16681 gtgccgtcag cgattgcttc gatctttatc tgtgcattcc gtccggacgc cgtatttggt
    16741 gcagcagccg gtattaccgt gcggaaagcc atgcgttacg gtgttgcccg aggcctcttc
    16801 tctaatgaag ccggtatggg ttccaccccg catgcccatg cgattgccga tgtcgagaac
    16861 ccggaggatc agggtgaagt agcgatgatt ggtgtcttta ttgatacctt cgttgtcctg
    16921 accatgaccg ctctcgttat tctttcttcc ggcgttctgg aagagatgat gagtaccggt
    16981 gttaaaggaa ccccggtcgc acaggcggca ttccgaaccg gcctcaaagg cttcggaccg
    17041 gcgttcgtag cgatctgtct tctgttcttt gcattctcga ccatcgtcgg atggtacttc
    17101 ttcgcaagac agaacgtaca gtatctgttc ggaaagaaag cagtcgttcc gttctcgctg
    17161 atcgtcgttg tgttcgtttt cctcggctct ctgctgaaag tggatctcgt atggaacctg
    17221 tcagacctct ttaacggcct gatggtaatt ccaaacctca tggcgttgat cgtcctcgca
    17281 ggaaccgtag caaaagcggc aaaaggtgaa aaagtagaat tctaatcata tatacagaac
    17341 gggaaaaagg aggaaagtct gctgatgcag actttcctcc tttttgcaat tattcagagc
    17401 cacatctcga ttccgctgcg ctgcatctcg atgtggcttc tcgccatata aaatttcaag
    17461 aagaactgtt cgttcatgcg agcatgactc acagttcttc ttgaaatttt gcatggctct
    17521 gaataattgc aaaaagaaag accggcaggg gagctaccga tctttcaagg ggagtataaa
    17581 taattaataa ggggttatta attgtaaaaa aatttatgaa gggtaaattc ttttacaaaa
    17641 ttgattataa tacagataac gggaaaaagc aacatcgaaa ttcaaaagtt ggaaaaaatt
    17701 accacaattt ggaaagcagt ggtccgaaag caggaaaaac actgtatttt tgggcttgca
    17761 ttccccactt tggaacagat accgtggaaa aacagggttc tggatgcata atctgcgcgg
    17821 aaaaagcgat actaaaggat gtcgcaggac aaaaaacatt caggaggaaa cgagattatg
    17881 accagaaaca gcaaaaatga atacagcaaa aacgagtaca gcaaaaacgg atacgagaaa
    17941 agcgaataca gcaaaaacca gaagaacagc agagactgca atcatgtgaa agaccgtgag
    18001 atgtcatcct acggtcagca gaaccgggag caggacgaga aggatgagta tgaaaaatac
    18061 taagtaaacc ttgggattcc ggcagaaaaa gccggcggag ggtcccatac ggactctgcc
    18121 gccggtttct gcatacatat aaaacaggag agaaagtcag atccggagag ggggaccagg
    18181 atatggcagg catgacactg ctggcggcag accagctgga ctggtacgtg ggaaaaccgt
    18241 gggcgatgat catcgatgtg cggaaccggc gggaatacga aaaagggcat gtgaaaggag
    18301 cggtgaatct tccctatgac catctgctcg aacgggtctg tctgccgcgg gacaaaattc
    18361 ttgtgatctg ctgtgagcgg ggtgccgcga gcatggcgga gggaaagcgg ctggcggaga
    18421 acggatacga ggtcaaaacg gtcatcggcg gaatgcatgc ataccgcgga cgatatctgg
    18481 tggcgggaga agagctgtaa gggggcggca atccaacatt tcttcttgtc aagcgggaaa
    18541 agatactgta agatgaaaca gaaaacaata caaaaccaaa ccacaaaacg aaagagagat
    18601 cacgatatga aatacatgtt tgcatctgat attcacggtt ctgcctatta ctgcagaaaa
    18661 atgctggaag ccttcgaggc agagggcgca agccgcctgt ttctgttagg cgaccttctc
    18721 tatcacggcc cgagaaacga tcttccaaaa gaatacgcac cgaaacaggt catcgccatg
    18781 ctgaatgaga aaaaacagta cctcaccaac gtgcgcggaa actgcgacgc agaagtagat
    18841 cagatggtac tggagttccc ggtgctggca gattacggca tcctgatcga gggaggccat
    18901 accttctata tgagccatgg ccacatctac aatgagcaga atctgccgcc gctgatggat
    18961 ggagatatct tcctgtacgg ccatacccac gtcctgcgtg ccgagaaaaa aggcgcacat
    19021 accttcctga acccgggttc tgtatctctg ccgaaagagg gcaatatccc gacctatgca
    19081 atcctcgagg acggcgtatt tacgatcaaa ggctttgacg gcaccgtcgt aaaacagctc
    19141 acattataag aggggacggg agagtatgga attatatgaa ctgatcgccc cgtgccactt
    19201 cggcctggag gcggtgatga aacgtgaaat cctcgacctc ggctacgaaa tcgagcgcgt
    19261 ggaggatgga aaagtaacgt ttcttgcaga tgcagaggga atcgcctacg ccaacctgtt
    19321 ccttcgcacg accgagcgta tcctgctcaa agtaggacag gtccacgccg agacgtacga
    19381 tgaactcttt gaagcgacaa aagcgctccc gtgggagaaa tttatcccga aaaacggaaa
    19441 gttctgggta acgaaggcaa actccatcaa gagtaagctc ttcagcccgt ccgatatcca
    19501 gtcgatcatg aaaaaagcga ttgtcgagcg attgaaacag gtctaccaca tggagtggtt
    19561 cccggaggac ggtgcacagt acccgatccg tgtttcgatt ttaaaagacg aggttacgat
    19621 cggactcgat acctccggcg tttcccttca taagagaggc taccgcaagc tgaccgcgaa
    19681 agcgccgatc accgagaccc ttgccgcagc acttatcatg ctgactccgt ggaagggtga
    19741 ccggattctt gtcgatccgt tctgcggaag cggtacgttc ccgatcgagg cggccatgat
    19801 ggcggccgat attgcaccgg gtctcaaccg ttctttcctt gcggagcagt ggacgcacct
    19861 cgtgccgaaa aaatgctgga tggatgcggc ggaggatgca cgctaccgga tccggaacga
    19921 tattgagaca gatattcagg gctttgatat cgacggagat gtcatcaaga gtgccagaga
    19981 gaacgcaaag ctggccggtg tggatcacct gatccatttc cagcagcgtc cggttgccga
    20041 gctgtcgcac ccgaaaaaat acggttttct gattaccaac ccgccgtacg gtgagcgcct
    20101 ggaggacaaa aaagatcttc cggccctgta tcagacatta ggagagcggt accgcgctct
    20161 cgattcctgg tcgatgtatc tgatcacgag ctacgaggat gccgagcggt atctcggcaa
    20221 aaaggcggat aaaaaccgta aaatttacaa tggaatgctg aaaacctact tttaccagta
    20281 cgcgggaccg aagccgccgc gccgggaaaa agaacagaaa taatacaaat ggaggagcaa
    20341 tgccgaatga tttttggcgt tgctcctttt tgataacaaa aattaaaaga aaaattttag
    20401 aaaagtttaa tatttccttg aaatcactgc actactgtgc taaactgtcc tcatgcgaaa
    20461 ttttttcatc aagcggagga aaccatgaac ggacaggaga tcagagacag gctgctgcat
    20521 tcctacgaga ggagctacga tatcgtaaag ccgtccgaag taaacggcca tacatacgat
    20581 gcctgtgcaa gctaccacga gagcggcgca aaatacgtgc tctcaaagaa agcagagctg
    20641 tggaggatca gctgtcatga gcatgcgtat tttaaagcgg tggaagaact gaacgaccag
    20701 gacgtcgaaa catttctgac ggacctgaca gaatggatag agccaaaggt ggttcgcgag
    20761 gggaaggaag taccggatac cgaccacatg tacacgctgg tcaccggtat tttttttgcg
    20821 gacaaaccgg ttgcggacag cgtaaagaag aaaatccgaa aatataagta ttataaaaat
    20881 taccggtttt ctttgagagg ctactgtcag gtgcggctgc tcgtctttga tacggcagaa
    20941 cagaaagtgt ttggaaaccg ggcggcttgg gaattggtaa agggatatgc taagatgttc
    21001 cgatcttagt tatctataaa tttattctac caaaaagagg gaggaaaaaa caaatgaata
    21061 gtattcttgt actggttgtc ggaatcggca ttctgatcgc cggatacgta ttctacggaa
    21121 aatggcttgc cgagcagtgg ggcgttgatc cgaaacggaa aacccctgca ttcaccgagg
    21181 aggacggtgt ggactacgtt cctgccaaag ctcctgtctt aatgggacat cacttctcat
    21241 ccatcgcagg cgcaggcccg atcaacggac cgattcaggc cgctgtcttt ggatgggttc
    21301 cggttctttt atgggtactc atcggcggta tcttctttgg aggcgttcat gattacggcg
    21361 cactgtttgc atccctgcgc cacaaaggac agtccatcgg cgaggttatc gcagacacca
    21421 tgggaagtaa agcgaaaaaa ctctttatcg tcttcgctta cctgaccctg attctcgttg
    21481 ttgcagcctt cggttccatc gttgccaata ccttcaaggc agcctacacc gagagcggcg
    21541 ctgtcgatgt ggcagcaagt tctgcaaatg caacaaccgc tatgatttcc attctgttca
    21601 tcgtactcgc cgttgtgttc ggtttcatgg tatacagaag aaatgtatct cttggcattt
    21661 ccaccatcgc aggcgttgca gctatcgttg tctgcgttgt tgttggatta aacttccatc
    21721 cgatctatct ctccgagaca gcatggatgg taatcgttgg tatctatatc accgtagctt
    21781 ctgttgcacc ggtctggatt ctgcttcagc cgcgtgacta cttaagctcc ttccttctgt
    21841 acttcatgat gatcgtagcc gctgtcggtg tcatcggaag tgctctgatg ggccatgcat
    21901 ccctggatat cccggcattc accggattca aagatacgct ggctccgacc ggaagttctc
    21961 ttggatttat gttcccggcc ctgttcgtaa ccatcgcctg cggagcaatt tccggattcc
    22021 attcactcgt tggttcagga actacatcga aacagcttga caatgagaaa aatgcccgcc
    22081 cgatcgcata cggcggtatg ctgattgagt gtgcacttgc aatcgtatct ctgtgtgcag
    22141 tcggctacat ctggtccaga tacgcagacg gaaccaccgt tgttccgacc gctgtatttg
    22201 caaccggtat ctccgagatg gtagcaacca ttccgggact tggcggatcg acccatgtac
    22261 tgtactctct gctggttctg actgtatcgg tattctgcct gacttctctg gataccgcaa
    22321 cccgtctggc aagatacatg ttccaggagt tctggcttga gccgggacag acttacaaag
    22381 aggcaaccgg ctacaaagcc gttctgacca atccggttgt atcaaccgtt atcaccgtag
    22441 tactcggcat cggccttggc ctgaccggtt attccaaaat ctggccgctg ttcggtgcat
    22501 ccaaccagct gctggcagcc atcggacttc ttgctgttgc cacctggctt ggaaaagtcg
    22561 gcaaaaacaa caaaatgttc ctgttcccga tggtattcat gctgatcgta accatcacat
    22621 cccttgtaca gaccctgatc gcaaacttca agggccttgg cgcaggcgtt ggcatcggct
    22681 ggtgcgtagc acgtatcgtg ctggcaggcc tgcttgtcat cctggcgctg atcctggctg
    22741 tcgatggatt caagactctg gcaaatcaga aaaaagcaaa ataatgtgtg attacaaaaa
    22801 aggctcccgg ataaaaacgg gggtcttttt ttgtgacagg gttcttgtgt ttgcgtcaca
    22861 catacggtat aatgatcgca agttatttca gcgtgaaaga aggaaaacgg ttatgaaaaa
    22921 aattgtattt gcaacaggaa atgccggcaa aatgaaagaa atccgtgaaa tccttgcgga
    22981 tctgccggtg gaaatttatt cgatgaaaga agccggcatt tcggtcgaga tcgatgagaa
    23041 cggcaaaacc tttgaggaga atgcaaaaat caaagcacag accgttgccg gatgcacaga
    23101 cggcgacacg atcgtgctgg cggatgactc cggtctggaa gtggatgcct taaacggcga
    23161 gcctggtgtc tattcggcaa gatatatggg agaggatacg tcctaccgca tcaaaaatca
    23221 gagcctcgtt gaccgcctgg agggcgtgcc ggtggaaaaa agaacggcac gattcgtctg
    23281 tgtgattgca gccgccttcc cggatggaac agtctgtacc accaaaggaa cgatcgaggg
    23341 aaaaatcggt tacgaggaac gcggggagaa cggttttggc tatgacccga ttttctatct
    23401 gccggatatg agccgcacga ccgcagaact ttctccggaa gagaaaaacg cagtcagcca
    23461 cagaggaaaa gcactggcgg cgatgaaaga acagattgca tcatatttga aataagaaga
    23521 gggaagagaa tgagtatgaa atttctgata gtgagcgata cccatggacg gatgtataac
    23581 ctcgaggagg ccatggaacg tgaggagttc gacggcatga tccactgcgg tgacgtggaa
    23641 gggatggatg acctgatcaa atccaaggta aacggcccgt gctatatggt catgggaaat
    23701 aatgactggt tttcaaacct cccggcggag attcaggtga acattgatga ttaccgtgcc
    23761 tttatcacac acggtcataa ctacggcgta tcgctcggcc tggagggaat ctatgcggag
    23821 gcgctttcta ggggtgtgga tatcgtactg ttcggccata cgcaccggcc ggtcgcagaa
    23881 aagcgtggaa atctctggat tgtcaatccg ggcagcctca cgtatccgcg tcagatggga
    23941 agaaaaccaa gctatgccat gctgacgatc aacgaagagc atgagctttc ggtggaaatc
    24001 aagtatctgg attaaaaaac gggtcgaaaa agggcggaaa acagcccaaa aacgaatgaa
    24061 ttatcgcaac taacatgact attccaaaga aaacagggta gtaccggagc aggttctgac
    24121 taatccttaa aaagtttgtc aaaaaaatac aatttaggtg ttgacagatg agaaaagata
    24181 tgataagatc tctttgttgt ccggtgagaa caaaacaaca caaaaaacat tttgaaaaaa
    24241 atgaaaaaaa gtgttgacaa accgaaaaca atgtaatata ataaatcttg cgtcgagcgc
    24301 gaaagggtta aacgcaaatc gggaaacctt gtttacgggg tgtggctcag cttggctaga
    24361 gcgcctggtt tgggaccagg aggtcgcagg
//
LOCUS       DAOMUF010000011        23997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_313_length_23997_cov_3.721201, whole genome shotgun sequence.
ACCESSION   DAOMUF010000011 DAOMUF010000000
VERSION     DAOMUF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 23997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23997
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_23997_cov_3.721201"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLN77_01665"
     CDS             <1..753
                     /locus_tag="PLN77_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HRM43022.1"
                     /translation="IYEAVRHLIETKTPHRPIEILISTGEELYCKGANAFDYSQVQAK
                     EAYVLDLSGAIGAAAYAAPTLVSFAAKIQGKAAHAGFHPEDGINSIQAAACAIAQLPQ
                     GHVDESTTANIGEIGGGSGTNIVSESCVIEGEIRSLNHETAMELLETYYRTFQQEAEK
                     IGAKLVWEEKLNIHAYETPLTSDAARHYESAVAEEGLTAAFEKTFGGSDNNVFAQHGI
                     EGLVIATSMNQVHSCAEYTKIPEIAQVAPQEA"
     gene            1113..2087
                     /locus_tag="PLN77_01670"
     CDS             1113..2087
                     /locus_tag="PLN77_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRM43023.1"
                     /translation="MIHTPIQKLEFPNDRNVRLYCKREDLLPFSLGGNKVRIGRAFFC
                     DMQEKNKDCMIIYGNSRSNLCRVLANLCCAEKIPCYMICSSEENEEQPIETNNSRLMK
                     WLGAEVIPCRKTEIAQTVEQTMNRLTEEGYHPYYIFGDKFGTGNEGTPVQAYVDGYRE
                     ILAWEKEQNISFSHIFAASGTGSTQCGLVSGKLLEQGTEQIIGLSISSREYDRAIRIM
                     ESGISDYFAKNHLVLPEGWESELHLEMGYRQGGYGQYDERILQVIREEFCRNGLPLDP
                     TYTGKAFWGMKQYIEEHQLQDCDVLFIHTGGTPLFYDCILNDTDGGKK"
     gene            2084..3031
                     /locus_tag="PLN77_01675"
     CDS             2084..3031
                     /locus_tag="PLN77_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM43024.1"
                     /translation="MKLISIVIPCYNSEATIRKVVEMVMEEFKKMDGYDCEFVLVNDG
                     SPKDNTYGEIKKLGEQYQNVKGINLLRNFGQHNALMAALHYTEGDFILGMDDDMQTHP
                     SQISKLIHKMEEGYDLVYGCYPKRKNSFLKNVTSKLNEVSSRILLGRPKDIVSSNFWM
                     ITRQIKDEVVKYDSFNPYIDGIFYRTTHKIGNVEVEHFKRAVGTSNYTLKKLIKLWLA
                     YWNYSVIPLRISSVLGGVSAAGGFLAAIIIAIRKLIDPTVMVGWSSTICAIVVFSGLI
                     LMVLGIIGEYIGKMILILNRTPQYIVRETINVSDEEEKK"
     gene            3028..4248
                     /locus_tag="PLN77_01680"
     CDS             3028..4248
                     /locus_tag="PLN77_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRM43025.1"
                     /translation="MKKIMILGAGIYQVPLIRTARRMGLYTIVVSIPGDYPGFALADK
                     IYELNTRDKEAILAAAEKEQIDGICTSGTDVAVSTIGYVCEKMHLSGIPYEAAEILTD
                     KAKMKDAFRQGGVSAADGMRVRSAEEAQKAAEQLGYPVVVKRVDSSGSRGITVVEHSG
                     QIEEAYENARNGSARDYVLVEKFLRGTEIGVDGFVQNHKLVFLAPHTKFVYRGAHTTV
                     PVGHAFPYGCSGALREEIARQMQLAVTASGADQCSVNADVFVDGEKVWIIEMGGRTGA
                     TCIPELISTYYGFDFYEQMIKSALGEETDFQQTESCPCMAKLLLSPVSGTITQIDRDQ
                     VERLRQEGLELVLDYPEGHEVSAMADGTDRIGHVIVKTDREAELDEQMKRVYRCIWID
                     GKNLETIWEEKTAK"
     gene            complement(4344..5444)
                     /locus_tag="PLN77_01685"
     CDS             complement(4344..5444)
                     /locus_tag="PLN77_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM43026.1"
                     /translation="MTKSRFLSGMRKSIAVSTLLSIAFTVVLTLVCCLLSRQILVWMQ
                     IPDDIAEEANAYMFVVLLGTGATVFYNMISNMLRALGDSKTPLYFLVFSSVLNIILDI
                     VFIVPMHMGVAGAAWATILSQFLSAVFSTVVGLRNFPILHLRREDFHDLKGAAVLHLK
                     TGFPMGFQMSVMCIGQLAMQAVVNSLGTAAVAGYTAASKADQLSVLVNNAMMTAISNY
                     VAQNFGAGKKDRIRLGVRASLIQTEAFNILMCIGILLLRHPIVELFLSNPTAEIYHYS
                     DAFLTIEAPFYFLLGLLAVYRTSIQSMQNGRAPFAACMIELVMRLAATVWLSRFLGYT
                     AVCIASPLAWFGAIALLIPVYYRMMIKPVKTV"
     gene            complement(5455..5682)
                     /locus_tag="PLN77_01690"
     CDS             complement(5455..5682)
                     /locus_tag="PLN77_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43027.1"
                     /translation="MAFNPMKLMELKNLRDRFAQNHPKFVKFMGDLASSQIEEGTILE
                     VTVKKPDGRTMVSNIKVTASDLEMLQAIKQM"
     gene            5833..6288
                     /locus_tag="PLN77_01695"
     CDS             5833..6288
                     /locus_tag="PLN77_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43028.1"
                     /translation="MKAKKYLLFFLLGYCIYIAIEVTYRGYSFRLMGIAGGLSLAGIG
                     MLEDAGLRRFPLAFQMMVSAVLITAIELYSGMFSLRVLHYRMWDYSALPFSFCDGLIC
                     PWFFGIWYLLSAVGIFLTDYVSYYIFHEETHPVYRKKKKGCAEGEFVIK"
     gene            6317..8266
                     /locus_tag="PLN77_01700"
     CDS             6317..8266
                     /locus_tag="PLN77_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HRM43029.1"
                     /translation="MENEKNYLEYLAELYPTIEKAATEIINLQSVINLPKGTEHFLSD
                     IHGEYEAFSHVLRNGSGAVRKKIDDVFGHTLGISEKRALATLIYYPRERLEMEKKQQE
                     DIDDWYKVMLYRLIDVCKIVSSKYTRAKVRKALPADYGYIIEELITEKPEVLDKEAYY
                     EAIIQTILEIGSAENFIIAVAELIQRLVVDHLHIIGDIFDRGPEPYKIMDCLMNYHSL
                     DIQWGNHDVLWMGAATGQPACVASTIRICLHYGNLDVLEDGYGINMLPLATFALETYG
                     DDPCECFAAKGGEDSGNSQQMLERRMHKAITVMQLKLENRLIRENPEYGMENRRLLEK
                     IDYQNGTVTIGEKTYALRDKSFPTIDPAHPDELTEKEAEVLDKLIFAFRNSEKLQAHV
                     DFLLKKGSLYRVYNGNLLYHGCMPMNEDGTLKEVQVDGKKYKGKALYDILEHNVRRAF
                     VSRDPKKREQGRNTLWYLWTAPNSPLYGRDKMTTFERYFLAEKETWTEVKNAYYRLIE
                     KEETADRILQEFGLAGENVHIINGHVPVHQSAGESPVKCGGKVLIIDGGFCRAYHKET
                     GIAGYTLIYNSYGLSLTAHEPFESTEKAIQEEKDIVSRQVAVRYNMKRQLVGDTDQGR
                     QIRQRIRELKELIEAYRTAQLKELL"
     gene            8367..9416
                     /locus_tag="PLN77_01705"
     CDS             8367..9416
                     /locus_tag="PLN77_01705"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRM43030.1"
                     /translation="MRAWIARMTEDNIDQSAIEKAGEILKNGGLAAFPTETVYGLGGN
                     ALDAKASEKIYAAKGRPSDNPLIVHIGRMEDLETVAANVPESARKLAAACWPGPLTMI
                     FEKTNAVPLETTGGLTSVAVRYPSNKVANALILAGGGFIAAPSANTSGRPSPTKAEHV
                     IEDLGDKIDCIIDGGDAEIGLESTIVDFTEEIPTILRPGYYNKEMLEKVLGTVRVDPG
                     ILAEDSHVRPKAPGMRYKHYAPKADLTIIQGEMERVIPEINRLAAEQEKAGKKVGVIC
                     TDETREQYTTGDIKSIGLRAEDETIAHHLFAILRDFDEDGVEVIYSEAFDTPRMGQAI
                     MNRLLKAAGHKVAEV"
     gene            9419..9877
                     /locus_tag="PLN77_01710"
     CDS             9419..9877
                     /locus_tag="PLN77_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotyrosine protein phosphatase"
                     /protein_id="HRM43031.1"
                     /translation="MKHYSRILFVDADDTARAPMAKAILQSKELPWPMEIGSRGLVVL
                     FPQPVNQKVEAVLARNGLSAKDHTTTPLTAEDINDDTLLLVMEDSQREKIREDFPDAP
                     HLQTIAGFLRLAGDIPALYGEPLTEYGKCYEALEFLIEGVLARLKEEEEQ"
     gene            9874..10317
                     /gene="rpiB"
                     /locus_tag="PLN77_01715"
     CDS             9874..10317
                     /gene="rpiB"
                     /locus_tag="PLN77_01715"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRM43032.1"
                     /translation="MIALGCDHGGYELMQEVKAYLDKQGLEYQDFGCYSNEAVDYPVY
                     ARKVGHAIQNGTCDKGILICGTGIGISITANKMEGIRAALCTDCFCAEATRLHNDANV
                     LAMGGRVVGPGLAIKIVETFLNTPFSGEERHERRIHLIEHEGENE"
     gene            10342..10971
                     /gene="upp"
                     /locus_tag="PLN77_01720"
     CDS             10342..10971
                     /gene="upp"
                     /locus_tag="PLN77_01720"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRM43033.1"
                     /translation="MARTVIMDHPLIQHKIGIIRKKDTSSKEFREMISEIAMLMCYEA
                     TRELPLTDVEIETPICKTTVKELEGKKMAVVPILRAGLGMVEGMLAMIPAAKIGHIGL
                     YRDPETLAPVEYYCKLPSDIASREVFVTDPMLATGGSATAAITMLKEKGAKKIHFMCI
                     IASPEGIEKMHEVHPDVDVFVGALDDHLNEHGYIVPGLGDAGDRIFGTK"
     gene            11015..11503
                     /locus_tag="PLN77_01725"
     CDS             11015..11503
                     /locus_tag="PLN77_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRM43034.1"
                     /translation="MSDKREDYISWDEYFMAVAQLAGMRSKDPHTQVGACIVSEDNKI
                     LSMGYNGFPKGCSDDEFPWGKTSENPLENKYLYTTHSELNAILNYRGGSLEGAKLYVT
                     LFPCNECCKAIIQAGIKTVIYDCDKYADTPSVIASKRMMKASGVTFQKYHRTGREITM
                     SL"
     gene            11598..12497
                     /locus_tag="PLN77_01730"
     CDS             11598..12497
                     /locus_tag="PLN77_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM43035.1"
                     /translation="MESNFEWITANGDIGIRFFHSIDDGGYSNPPHWHNDLEIIYLLE
                     GRLKVRFGDGGERQIGADELLVINSGTIHAVTAYPNQAIVLQVPYEMLLQYMEHYDQL
                     YFVVDERPASEAEKQKLAEFKAVLREMDEVYGAQKEGYQLKFDSLLLEALYRLLVDYA
                     VRIGKEHIPQNQKAVQQIREVMRYIEEHYAEKLTVAQVAEHFGYTPNYLSKLLKRYLG
                     LTVYQYLYEIRLDRICRDLQHPEARIMELLPKHGCENYKSVSRLFKERYGCSMKEMQK
                     RYEKEKGDRPMGQWVRIRKAEMP"
     gene            12611..14080
                     /locus_tag="PLN77_01735"
     CDS             12611..14080
                     /locus_tag="PLN77_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HRM43036.1"
                     /translation="MKAKQITTYHVKGEAKTWEKALAPKDESKSVKMIESNVINLYPD
                     FAFQTIEGFGGAMTESSAYLLSRMDEETQNQALQDIFGPDGLHARFVRVPIDSCDYSL
                     EEYQAVADPIADPDLATFSIDRDRKYVLPMLKKAIEISAEPISVLMSPWSPPYQWKTA
                     PKIAKNDAAVYGAMGMPVPEEIPQRNHGGSLKPEYYGSWAKYVVKYLQAYLDEGIPVT
                     MLSLQNETVAATTWDSCVWTPEESKTYLKDYLYPEMKKAGLTDKIGIFIWDHNKERMI
                     EHVLTVLDEETMDMVAGIAFHWYSGDHFEAVELMKQKYPDKTFMLSECCALHMPGRIG
                     FGDGGFGPVNFQTPEYVDYLDAADYAHDMIGNLNAGMNRWIDWNFLVDKDGGPRHVPM
                     GFTSGLIVDDDFHYRKPIMYHYIGHISKYIAPGAKRIGWSKYGANLDVTAAVNPDGSY
                     VVILLNRTKEDSGCFLRVNGHIMRVDLPAETLSTVVIEK"
     gene            complement(14156..15565)
                     /locus_tag="PLN77_01740"
     CDS             complement(14156..15565)
                     /locus_tag="PLN77_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM43037.1"
                     /translation="MKLDRYRKYVRAGVTAFLVLAASMIFYYLLFHQTSVRTAFHNVL
                     NILSPLIAALILAYVISPIINFIEQRIIARLFARHGKTQSVRCKKLVRALLILLTYVS
                     MLFALYGLIATLIPELFSSIANIVSNFSNYANNIQDFATKVLANYPEMQQTADQYIST
                     FSAKLSNWLTNDLMPMLRTFLLNLSGQLVNMVTFFKNILLGAIVAIYMLYNKESYIAK
                     LKKAVYALLGLDHGNAVIRDCQYVNQEFSGFLVGKVIDSIIIGAICYLVTSLIGTPYA
                     LLVSVIIGVTNIIPFFGPFLGAVPCLLLILLIDPLQALYFLIFVVLLQQFDGNFLGPK
                     ILGETTGVSSFLIIISILIGGGFWGVFGMIIAVPVCAIICTVCRNFANRRLEKHHLPD
                     DDAFYFHMDHIDPETRQAIPYQKKKIDEAEVFHFPGYRLRKALTKLTTEESPVDAPYT
                     PAKPSTEEKNSDSSDSDQK"
     gene            15707..17035
                     /locus_tag="PLN77_01745"
     CDS             15707..17035
                     /locus_tag="PLN77_01745"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRM43038.1"
                     /translation="MKIKMPPSVNMVIDSLHAKGFEAYAVGGCVRDTILAREPDDWDI
                     TTSASPQQVKEIFDRTVDTGLEHGTVTVLAGSGAHEVTTYRIDGEYEDGRHPKEVVFT
                     SSLAEDLRRRDFTINAMAYNNEEGLVDLFDGIRDLQRKVIRCVGNPTERFSEDALRIL
                     RALRFSAQLGFKLDPATLDAIIALAPTLEKISVERIRVELNKLLISKRPEYMEAVYEA
                     GITKIVLPEFDELMKTPQNNPHHCYNVGEHTIAALQHVPADPILRWTMLLHDIAKPAC
                     RTTDEAGIDHFKGHAPAGEKMAKAILRRLKFDNETIRQVAALIRWHDCRMAPEKPVIR
                     RILNKLGPELFEKLMIVQEADTMAKSLYQREETLERIGKVRECVREILDNGDCFTLKT
                     LAVSGKDLMELGVPKGPKIGECLNAALEEVMKDPAKNTKEYLTEYVKTLL"
     gene            complement(17108..18973)
                     /locus_tag="PLN77_01750"
     CDS             complement(17108..18973)
                     /locus_tag="PLN77_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRM43039.1"
                     /translation="MIFLPQPSSLSYGEGTFTIHYDSRIFLDSESPAELFSAAQLLQQ
                     EIETQTGFRPAICRRHQPVGSHLIYLTASPELSREAYTLAVTPENITICGSLESGVLY
                     GVQTLRQMIRQAGAVLPTVLISDKPAMENRGFYHDATRGRVPTLSYLKQLADTLSFYK
                     INQLQLYIEHSYLFDDLTEMWRDDTPLTAEDILELDRYCKGLGIDLVPSLASFGHLYK
                     LLCTKSYAHLCELEGSASAPFSFYDRQAHHTLDITNPESLSLAKHILSEYMQLFSSKY
                     FNLCADETFDLGKGASRALAEEKGTTVIYTEFVTELANYITESGRTPMFWSDVISQEP
                     EVYHLLPKNLICLHWDYASNVSSERLTRLANSGAEHLYVCPGVQGWNQLINKYHEAYE
                     NISRMARYGHECHAMGLLNTDWGDYGHINHPDFSRIGMIYGAAFSWNADILPEEEINR
                     QISVLEFGDASGKLVSVLDLLCHQDAYPWRTAVMVQEALELHQDKEEAAELLRSCAEG
                     DADAANASIDALCAVLYEKAGTVRPENRPMIYAYLLAADGLKVLNRLLPFLRASLLSE
                     GTLPEKEDCFALAGDLERWLHSYKELWRTVSKESELYRIAHVFCWYADLLRDLNA"
     gene            19222..20199
                     /locus_tag="PLN77_01755"
     CDS             19222..20199
                     /locus_tag="PLN77_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HRM43040.1"
                     /translation="MYDYGLSVLSHYGLTASGTMRVRGALLCHTEQGDKLISEFGTAA
                     EKLEQRYLLQCRITESGLLRCDQILRNEEGALATRGDDGGTYIVRNWYPGRECETGNS
                     EDLIRASDALARLHTAAHLPVKMEYRRESLVEECIRHNVEIRKIRKYLQTKKKKNEFE
                     KLLLASAGPYLEQGERAAAEMKASSYEKLRQRADEEGAVCHGEFSQHNILLENGRGET
                     AVNFDKWNFDLQVADLGYFMRKILEKHGWSERAAERILNAYSARRPLDEGEIETLRLY
                     LSYPWKYWKLANRYYGSRKSWISGRNTEKLKTLNAQQADWLRFVKGSFF"
     gene            20441..20713
                     /locus_tag="PLN77_01760"
     CDS             20441..20713
                     /locus_tag="PLN77_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRM43041.1"
                     /translation="MNKTELVAAMAEQTNLSKKDAEAALKAFIDVVSEELKKGEKVQL
                     VGFGTFEVSERAAREGRNPQTGETMEIKASKTPKFKAGKALKDMMN"
     gene            20724..20963
                     /locus_tag="PLN77_01765"
     CDS             20724..20963
                     /locus_tag="PLN77_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRM43042.1"
                     /translation="MRLDKFLKVSRLIKRRTVANEACDAGRVLVNDRPAKASAQVKAG
                     DVLEIQFGSKSVRVEVLNVQETVKKEEAQELYRYL"
     gene            21051..21329
                     /gene="yabP"
                     /locus_tag="PLN77_01770"
     CDS             21051..21329
                     /gene="yabP"
                     /locus_tag="PLN77_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HRM43043.1"
                     /translation="MGEMNEQVHRLLLEQRKTLSLGGVQDVKSFDENEVLLVTVCGVL
                     TIRGKELHVGRLELEKGEADIEGSIDSLVYTEHGPLQKKGSALARLFR"
     gene            21332..21739
                     /locus_tag="PLN77_01775"
     CDS             21332..21739
                     /locus_tag="PLN77_01775"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HRM43044.1"
                     /translation="MSAYVTRELILLACSIWQGAVLGFWYDLIKTARKLIRHSHAAVS
                     AEDLCYWIAAALYLFVRIYGENSGILRGYLFGGILGGAALYHASFGRFLPELLAKGIK
                     KAGRILAFPGKWLVKSMKKRRKRLKFHGKNGKM"
     gene            21840..22169
                     /locus_tag="PLN77_01780"
     CDS             21840..22169
                     /locus_tag="PLN77_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRM43045.1"
                     /translation="MKEREEKNSGRKKKVRKKSRTTEFSITIVLCCLVAVLTVKGISL
                     QQKVNANASRLSSLDSQIEEEKQRTDDITALKEYMQTDEYIEKTAREKLGMVKDNEIL
                     FKESQSK"
     gene            22244..23725
                     /locus_tag="PLN77_01785"
     CDS             22244..23725
                     /locus_tag="PLN77_01785"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRM43046.1"
                     /translation="MPEWIIAMFLIGALLSAGDMAKIFLEARRSRREAAVYDNHPQKL
                     QMEHYADSFRVLAESFYQMPSKSVMPETGRVDKILEKEQQEVCSRCAKASWCWEQYGN
                     LTRERCQELLQTIADGDEDEISRAKGEWNASCLNGSRFLELFWNRYQQERQTALWSGR
                     VAESRRVVAEQLSEVAGIMDRAACDLYSLNSLPDELSEKICRQMKKNGAFVQKIWLQE
                     RPKEHLQIYMTVKAGRHCRITLRQTAELIGSLCGIPMVAVRDGAQVLSGEYQTVLFQE
                     DVKFQVLYGVGRITKEQESISGDNYSVLCENGQFVLCLSDGMGSGIEANRESETVVEL
                     FEQFLRAGFPRIMAARMINSMLLLQPKEGMFSTFDVASINLYTGVCSFLKGGASPTFL
                     RRDTWVEVVESTSLAAGLVSQTDFDTTTKKLYDGDYLVMMTDGVLDALPGDRTEQMKQ
                     LLLEVKNSEPREFARELLERVLRLGGCRARDDMTVLVARIWKK"
     gene            23741..>23997
                     /locus_tag="PLN77_01790"
     CDS             23741..>23997
                     /locus_tag="PLN77_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM43047.1"
                     /translation="MREKVRAFMEQYHMVTAGERVLLGLSGGADSVCLFHLLRELQEP
                     LGFSLLAVHVNHNLRGAEAGRDAAFAENLCRYAVVHGYSASP"
BASE COUNT         6599 a         5705 c         6818 g         4875 t
ORIGIN      
        1 atctatgaag ctgtccgcca cctcattgag accaaaacac cgcaccgccc gatcgaaatc
       61 ctgatctcga ctggtgagga gctgtactgc aaaggcgcaa atgccttcga ctacagccag
      121 gtgcaggcaa aagaagcata cgttctggat ttaagcggcg cgatcggtgc agccgcctac
      181 gcggcaccga cactcgtttc cttcgcggcg aaaatccagg gaaaagccgc ccacgccggt
      241 ttccacccgg aggacggcat caacagcatc caggcggccg cctgcgcaat cgcacagctt
      301 ccgcagggcc acgtggatga aagcaccact gccaacatcg gagaaatcgg cggcggctcc
      361 ggtaccaaca tcgtttcgga aagctgcgtg atcgaaggcg aaatccgaag cctgaaccac
      421 gagacggcga tggagcttct ggaaacatat tacagaacct tccagcagga agcagagaag
      481 attggtgcga agctggtctg ggaagaaaag ctgaatatcc atgcgtatga gacgccgctg
      541 acaagcgatg ccgcaaggca ttacgagagc gccgttgcag aagaaggcct gaccgctgca
      601 ttcgaaaaaa ctttcggcgg cagcgacaac aacgtctttg cacagcacgg catcgaaggg
      661 cttgtcatcg caacctcgat gaatcaggta cacagctgcg ccgagtacac aaaaatcccg
      721 gagatagccc aggtggcacc gcaggaagcc tgaacagatt ccggacgaaa aaacagaata
      781 cgtaaaaaat ccccaaagcc cgccggcaaa tgtgaaaaca aaaaccggcg ggcttttcct
      841 gtttccggca cggcttgcca cgcagagaaa aatagtctat aatagggaaa agcttccggt
      901 gcatgccgga atttaacacc agtagacaag aagtcccccg cttctaaagc ggtgggatga
      961 attgttaaaa gaaacataac ggcagtggcg ggcggagaga aatcggtatc ctccactgac
     1021 atgaggcaag tcacagtcga actattttcg acatgtcaga ctccgtgaaa ataccgactg
     1081 tgagttgaaa caagaataaa agaggaaaaa caatgataca tacaccaatt cagaaactgg
     1141 aatttccaaa cgacagaaat gtccgactgt actgcaagag agaggatctt ctcccgtttt
     1201 ccctgggcgg aaataaagtg cggatcggac gcgccttttt ctgtgacatg caggagaaaa
     1261 acaaagactg catgatcatc tacggaaaca gcagatccaa cctctgccgc gtgctggcca
     1321 atctgtgctg tgccgagaaa attccatgct atatgatctg ttcttccgaa gaaaacgagg
     1381 aacaaccgat cgaaacaaac aacagccgtc tgatgaaatg gctcggagcc gaagtcattc
     1441 cgtgcagaaa aacagagatt gcacagaccg tcgagcagac gatgaaccgt ttgacagaag
     1501 agggatatca cccatattat attttcgggg ataaattcgg aacgggcaat gaaggcacac
     1561 cggtacaggc ctacgtggac ggctaccgcg agattcttgc gtgggagaaa gaacagaaca
     1621 tttctttttc tcatatcttt gcggcatccg gaaccggctc gacccagtgc ggcctggtaa
     1681 gcggaaaact gctcgagcag ggaacggagc agatcatcgg cctttcgatt tcatcccgcg
     1741 agtacgaccg cgccatccgc atcatggaga gcggcatttc cgattatttt gcgaagaatc
     1801 atctcgtgct gccggaagga tgggagagtg aacttcatct ggaaatggga taccgccagg
     1861 gcggatacgg ccagtacgat gagcggattt tacaggtgat ccgggaagag ttctgccgga
     1921 acggccttcc gctggatccg acgtacaccg gaaaggcatt ctggggaatg aagcagtaca
     1981 tagaagaaca tcagttacag gactgcgatg tgctttttat ccataccgga ggcacgccgc
     2041 tgttctatga ctgcattctg aacgataccg atggaggaaa aaaatgaaac tgatttcaat
     2101 agtcatccca tgctacaatt ccgaggcaac gatccggaaa gttgtagaga tggtgatgga
     2161 agaattcaag aaaatggacg gatatgactg cgaatttgtc ctggtgaacg acggttcccc
     2221 gaaagacaat acgtatgggg agatcaagaa gctcggtgag cagtatcaga atgtcaaggg
     2281 catcaacctg ctgcgcaatt tcggccagca caatgcgctg atggcggcac tccactacac
     2341 cgaaggagac tttattctcg gcatggacga tgacatgcag acacatccgt cccagatcag
     2401 caaactgatc cacaagatgg aagaaggata cgatctggtt tacggctgct atccgaaacg
     2461 aaaaaattcg ttcctgaaaa atgtcaccag caaactgaac gaagtttcct cccggattct
     2521 tctcggacgg ccaaaagaca tcgtttccag caatttctgg atgattaccc gccagatcaa
     2581 ggacgaggtg gtaaaatacg acagcttcaa tccctatatt gacggcattt tctaccgcac
     2641 gacacacaaa atcggaaatg tagaggtgga gcatttcaaa cgtgcagtgg gaacttccaa
     2701 ctatacgtta aagaaactga tcaaattgtg gcttgcctac tggaactatt ctgtaattcc
     2761 gcttcgcata tcgtccgttc tcggcggagt ttctgcagcc ggcggttttc tggcagccat
     2821 cattattgcc atccgaaagc tcatcgatcc gaccgtgatg gttggctggt cttctacaat
     2881 ctgcgccatc gttgtctttt ccggattgat tctgatggtg ctcggcatca tcggagaata
     2941 catcggaaag atgattctga ttctgaaccg cacgccgcag tatattgtgc gggaaaccat
     3001 taacgtatct gatgaggaag aaaaaaagtg aaaaaaatca tgattttagg tgccggtatt
     3061 taccaggtgc cgctgatccg gactgcacgc agaatgggtc tttacacgat cgtagtgagt
     3121 attcccggcg attatccggg gtttgcactt gcagataaaa tttatgaact gaatacgcgg
     3181 gataaagaag cgattcttgc ggcggcggaa aaagaacaga tcgacggaat ctgtacatcg
     3241 ggaaccgatg tggccgtcag cacaatcggc tatgtctgtg aaaagatgca tctgtccggc
     3301 attccgtatg aggcggcgga aattctgacc gataaggcaa agatgaaaga cgcattccgg
     3361 cagggcggcg tttccgcggc agacgggatg cgggtaagaa gcgcagagga ggcacaaaaa
     3421 gccgcagaac agcttggata cccggttgtc gtaaaacggg tggacagttc aggaagccgc
     3481 ggaattaccg tggtggagca ttccggacag atcgaagaag cgtatgaaaa tgcgagaaac
     3541 ggttcggcaa gagattatgt gctggttgaa aaattcctcc gcggaacgga gatcggcgtg
     3601 gatggatttg tgcagaacca caaactggtc tttctggcac cgcacacgaa atttgtatac
     3661 cgcggtgcac acacgaccgt tccggtcggc cacgcatttc cgtatggatg cagcggggca
     3721 ctgcgggaag aaattgcccg ccagatgcag ctggccgtca ccgcatccgg tgccgatcag
     3781 tgctccgtca atgcggatgt ctttgtggac ggagagaagg tctggatcat tgaaatgggc
     3841 ggaagaaccg gggcaacctg tattccggaa ctgatttcaa cgtattacgg atttgatttt
     3901 tatgagcaga tgatcaaaag cgccctcggg gaagaaacgg attttcagca gacggaaagc
     3961 tgcccgtgca tggcaaaact gctgctgagt ccggtaagcg gaaccatcac acaaatcgac
     4021 cgggatcagg tcgaacgtct gcgtcaggaa gggctggagc ttgtcctgga ttatccggag
     4081 gggcatgaag tttctgccat ggcagatgga actgaccgga tcggccatgt gattgtaaag
     4141 acggaccggg aagcggaact ggatgagcag atgaagcggg tgtaccgctg tatctggatt
     4201 gatggaaaaa atctggagac aatctgggaa gagaaaacag caaaataaaa aacccggaat
     4261 cttttcaaga cctcagaaga ggagcgaaag gattccgggt tttgcgttgc caggcatatc
     4321 aatatccggt ggaccaattt tgattacact gtttttacag gctttatcat catccgatag
     4381 tagactggaa tcagcagtgc aattgcaccg aaccaggcaa gcgggcttgc gatgcagacg
     4441 gcagtgtagc ctaagaagcg tgacagccag acggtagcag caagacgcat cacaagctcg
     4501 atcatgcatg cggcaaacgg tgcgcgtccg ttctgcatac tctgaatcga ggtgcggtag
     4561 acggcgagaa ggccgagcag gaagtagaac ggcgcttcga tcgtcaggaa tgcatcggag
     4621 taatggtaaa tttctgcggt cggattcgaa agaaacagtt cgacgatcgg atggcgcagc
     4681 agaagaatac cgatgcacat cagaatgttg aacgcttcgg tctggatcag cgaagcgcgt
     4741 acgccgaggc ggatacggtc cttcttaccg gcaccgaagt tctgtgcaac gtagttagag
     4801 atcgccgtca tcatggcatt attcacaagg acggagagct ggtcggcttt ggaagctgcc
     4861 gtgtagccgg caaccgcagc cgttccaaga gagttgacaa cagcctgcat cgcgagctgg
     4921 ccgatacaca tgacggacat ctgaaagccc atcggaaagc cggttttcag gtgaagaaca
     4981 gcagctcctt tcaggtcgtg gaaatcctca cggcgcaggt gcagaatcgg gaaattccga
     5041 agtccgacaa ccgtgctgaa aacggcggag aggaactgcg agagaatcgt cgcccaggca
     5101 gcgcccgcaa cgcccatgtg catcggaacg atgaacacga tgtcgaggat gatgttgagc
     5161 accgacgaga acaccagaaa atacagcggt gttttgctgt cgccgagtgc gcggagcata
     5221 ttggaaatca tgttgtagaa cacggtagcg ccggtgccga gcagaacgac gaacatatag
     5281 gcattcgctt cctctgcgat gtcatccgga atctgcatcc agacgagaat ctggcgggag
     5341 agcagacaac agacgagcgt cagcacaacg gtgaacgcga tactcagcag cgtggaaact
     5401 gcgatgcttt tgcgcatgcc ggaaagaaag cggctttttg tcacgaaaaa cgtgttacat
     5461 ctgtttgatt gcctgcagca tttcaagatc cgaagcagtc accttgatat tggaaaccat
     5521 tgttcgcccg tccggctttt tcaccgtgac ttcgagaatc gttccctctt cgatctggga
     5581 ggatgcgaga tcgcccatga attttacgaa cttcgggtgg ttttgggcga aacggtctct
     5641 cagatttttt aattccatta acttcattgg gttaaatgcc attctttttt ctgtcctttc
     5701 tattcgaaac ttcttttgaa acatgaaagt attgtaacat ggaaaagaaa aaatgcaagc
     5761 ggataaaaag agaaaagaca gagatactgg gaataagaga aaagattctc aattagaatg
     5821 ggagaaaaac gaatgaaagc gaagaagtat ctgctgtttt ttcttttggg ctactgcatt
     5881 tatattgcca tcgaagtcac ctaccgcggt tacagcttcc gcctgatggg cattgccgga
     5941 ggactgtccc ttgcaggaat cggaatgctg gaagacgccg ggctgcgccg ctttccgctg
     6001 gcgtttcaga tgatggtatc tgcggttctg atcacggcca tcgaactgta ttcgggaatg
     6061 ttttccctgc gggtgctgca ttaccgtatg tgggactaca gtgcgctgcc gttttccttc
     6121 tgcgatgggc tgatctgccc gtggtttttc gggatctggt atctgctctc ggcggttggg
     6181 atttttctta cggactatgt gagctattat atcttccacg aggagacgca cccggtgtat
     6241 cgaaagaaga aaaagggttg tgccgaaggg gagttcgtta taaaataaga aaaaacaacc
     6301 caggcaggag gtcctcatgg aaaacgaaaa aaattatctg gaatatctcg cagaattgta
     6361 cccgaccatc gaaaaagcag cgaccgaaat catcaatctg cagtccgtta tcaaccttcc
     6421 caaaggcacg gagcatttcc tttcggatat tcatggcgaa tacgaagcct tttctcatgt
     6481 actccgcaac ggttccggtg ccgtgcgaaa gaagattgac gatgtcttcg gccacacgct
     6541 cggcatttcg gaaaaacgtg cgcttgcaac ccttatctat tacccgagag agcgcctgga
     6601 gatggagaaa aagcagcagg aggacatcga tgactggtac aaggtcatgc tgtaccgcct
     6661 gatcgacgtc tgcaaaatcg tttcttccaa atacacccgc gccaaagtgc ggaaagccct
     6721 gccggcggat tatggctata tcatcgagga gctgatcacc gaaaagccgg aggtgctcga
     6781 caaagaagca tactacgagg cgatcatcca gactatcctc gaaatcggca gcgccgagaa
     6841 cttcatcatt gccgtggcag agctgattca gcggctcgtt gttgatcatc tccacatcat
     6901 cggcgacatc tttgaccgcg gtccggagcc gtataaaatc atggactgcc tgatgaatta
     6961 ccattccctc gatatccagt ggggcaacca cgatgtgctc tggatgggcg ctgcgactgg
     7021 ccagccggcg tgcgtggcca gcacgatccg catctgcctc cactacggca atctcgatgt
     7081 gctggaggac ggctacggca tcaatatgct tccgcttgcc acctttgctc tcgagacgta
     7141 cggcgatgat ccgtgcgaat gctttgccgc gaaaggcggc gaggacagcg gaaattccca
     7201 gcagatgctg gagcgccgca tgcacaaggc catcaccgta atgcagttaa agctcgagaa
     7261 ccgtctgatc cgtgagaacc cggagtacgg catggaaaac cgccgtctgc tggaaaagat
     7321 cgattaccag aacggaacgg tgacgattgg ggagaaaacg tatgctctgc gcgacaagag
     7381 ctttccgacg atcgacccgg cgcatccgga cgagctgaca gaaaaagagg cagaagtgct
     7441 cgataagctg atcttcgcct tccgaaacag cgaaaaactg caggcgcacg tcgatttcct
     7501 gctcaaaaaa ggaagcctct accgcgtcta caatggaaat ctgctgtacc acggctgcat
     7561 gccgatgaat gaggacggca ccttaaaaga ggtacaggtc gacggaaaga aatacaaggg
     7621 aaaagcactg tacgatatcc tcgagcacaa cgtgcgccgc gcatttgtaa gccgcgatcc
     7681 gaaaaaacgg gagcagggcc gcaatacgct gtggtatctg tggaccgcgc cgaattcccc
     7741 gctgtacggc cgcgacaaga tgacaacatt tgagcggtat ttcctcgcag aaaaagagac
     7801 ctggaccgag gtgaaaaacg cgtattaccg cctgatcgag aaagaggaga cggcggaccg
     7861 gatcttacag gagttcgggc ttgccggtga aaatgtacat attatcaacg gccacgtccc
     7921 ggtacaccag agtgccggtg aaagcccggt caaatgcggc ggcaaggtgc tgatcatcga
     7981 cggcggcttc tgcagggcgt accacaaaga aaccggcatc gcgggttaca cgctgattta
     8041 caactcgtac ggcctcagcc tcaccgcgca cgagccgttt gaatcgacgg aaaaagcaat
     8101 ccaggaggag aaggatattg tctcccgcca ggtggcggtg cggtacaaca tgaagcgcca
     8161 gttagtcggc gatacggatc agggacggca gatccggcag cgcatccgtg aattaaagga
     8221 actgatcgag gcgtaccgca cggctcagtt aaaagagctt ctttaaatca agggttttca
     8281 actgcgccgg aaaatggtat aatcaaaaca gtgtgcgcag atggaaacac tgtgcgctgg
     8341 aacagatgga aagaaggaag aaacatatga gggcatggat tgccaggatg acagaagata
     8401 acatagatca gagcgcgatc gaaaaagcag gagagattct gaaaaacggc ggtctggcgg
     8461 cattcccgac cgagaccgta tatggtcttg gcggaaatgc gctggatgcc aaagcttctg
     8521 agaaaattta tgcggcaaag ggccgtccgt cggacaaccc gctgatcgtc cacatcggaa
     8581 gaatggagga cctcgaaacc gtagcggcaa acgtgccgga gagtgcacgc aaactggcag
     8641 ccgcatgctg gccgggaccg ctgacgatga tttttgagaa aacaaatgcc gttccgctcg
     8701 agacgacggg aggccttacc tccgtagccg tgcgctaccc gagcaacaaa gtggcaaacg
     8761 cactgatctt agccggaggc ggcttcatcg cagcgccgag cgccaataca tcaggccggc
     8821 cgagtccgac gaaagccgag catgtgatcg aggatcttgg ggacaaaatt gactgtatca
     8881 tcgacggagg agacgccgag atcggcctcg aatccactat cgtcgatttt acagaggaga
     8941 ttccgacgat tctccgcccg ggctattaca acaaagagat gctggaaaaa gtgcttggaa
     9001 ccgtgcgtgt cgatccggga atccttgcgg aggacagcca tgtgcgtccg aaggcaccgg
     9061 gcatgcgcta caaacattat gcgccgaagg cagaccttac gattatccag ggcgagatgg
     9121 aacgggtgat tccggaaatc aaccgccttg ccgcagaaca ggaaaaggca ggaaagaaag
     9181 tgggcgtaat ctgcaccgat gagacacggg agcagtacac cacaggagat atcaagagca
     9241 tcgggctgcg cgccgaagat gagacgatcg cgcatcacct gtttgcgatt ctgcgcgact
     9301 tcgatgagga cggcgtggag gtgatttatt ccgaggcttt cgacaccccg cgtatggggc
     9361 aggcgatcat gaaccgcctg ttaaaagccg caggccacaa agtagcagag gtataacagt
     9421 gaaacattac agcaggattt tatttgtgga cgcggacgat accgcgcgcg caccgatggc
     9481 gaaggcgatt ctgcagagca aagagcttcc gtggccgatg gaaattggtt cccgcgggct
     9541 tgtcgttctg tttccgcagc cggtcaacca gaaggtagag gcagtgcttg cgcgcaacgg
     9601 gctttcggcc aaagaccaca ccaccacacc gcttaccgcg gaggacatca acgacgacac
     9661 gctgcttctt gtgatggagg acagtcagag ggaaaagatc cgtgaggact tcccggatgc
     9721 gccccatctg cagacgatcg ccggatttct gcggctggca ggagacatcc ccgcccttta
     9781 cggggaaccg cttaccgaat acggaaaatg ttatgaagcg ctggaattcc tgattgaagg
     9841 tgttcttgcg cggctgaaag aggaggaaga acaatgatag ctttaggatg cgaccacggc
     9901 ggatatgagc tgatgcagga ggtaaaagca tatctggata aacagggact ggaatatcag
     9961 gacttcggat gctacagcaa cgaggcagtt gattatccgg tttacgccag aaaagtaggc
    10021 catgcgatcc agaacggaac gtgtgataag ggaatcctga tctgcggaac cggaatcggc
    10081 atttctatca cggcaaacaa aatggaagga atccgtgcag cactctgcac cgactgcttc
    10141 tgcgcagagg caacccgcct gcacaacgat gccaacgtac tggcgatggg aggacgtgtt
    10201 gtaggccctg gccttgcaat caaaatcgta gaaaccttcc tgaacactcc attttccgga
    10261 gaagagcgtc acgaacgccg gattcatttg attgagcatg aaggagaaaa tgaataaatt
    10321 ttaaacaaag gagaagggaa aatggcaaga acagtcatca tggatcaccc gctgatccag
    10381 cacaaaatcg gtattatcag aaagaaagac acaagctcca aggagttccg cgagatgatc
    10441 agcgagattg caatgctgat gtgctacgag gcaaccagag agctgccgct cacagacgtt
    10501 gaaattgaaa ccccgatctg caaaacaacc gtaaaagagc tcgaaggaaa gaaaatggca
    10561 gttgtgccga tcctccgtgc cggtcttggt atggtagaag gtatgctggc tatgattccg
    10621 gcagcaaaaa tcggacacat cggtctgtac cgtgatccgg aaaccctggc accggttgag
    10681 tattactgca aacttccgtc agacatcgca agccgtgaag tatttgtaac cgacccgatg
    10741 ctggcaaccg gcggttctgc aacggcagcc atcaccatgc tgaaagaaaa aggcgcaaag
    10801 aagatccact tcatgtgcat catcgcatct ccggaaggaa tcgagaaaat gcatgaggtt
    10861 cacccggatg tcgatgtttt cgtcggagca ctggatgacc atctgaacga gcacggctac
    10921 attgtaccag gcctcggcga tgccggagac cgtatcttcg gtacgaaata agagcggaag
    10981 caaagtacgt taagaaaaca caggggaaat gggtatgagc gataaacgag aggattatat
    11041 ttcctgggat gaatatttca tggcagttgc ccagcttgcc ggtatgcgct cgaaggatcc
    11101 gcacacccag gtgggagcct gcattgtgag cgaggacaac aagattctgt ccatgggata
    11161 caacggtttc ccgaaaggct gctcggacga cgagttcccg tggggaaaga caagtgaaaa
    11221 tccgctggaa aacaagtatc tttacacaac gcacagtgaa ctgaacgcca tcctgaatta
    11281 ccgcggtgga agcctcgagg gagccaagct gtatgtgaca ttgttcccat gcaacgagtg
    11341 ctgcaaggcg attatccagg cgggaatcaa aacggtgatc tacgactgcg acaagtacgc
    11401 ggacaccccg tccgtgatcg catcgaaacg gatgatgaag gcgtccggag tcacttttca
    11461 gaaataccac cgcacagggc gggaaattac catgtccctg tagcagaaaa gataatagaa
    11521 ggagcgcgcc tgaacgaaca gaaagttcag acgcgcttcg tctgtataca cgaaaagaca
    11581 agaaacggga gggcaccatg gagagcaatt tcgaatggat tacggcaaac ggggatatcg
    11641 gcatccgctt tttccacagc atcgacgacg gcggctacag caacccgccg cactggcaca
    11701 acgatctgga gatcatttat cttctggaag gccgtctgaa agtgcgtttc ggtgacggcg
    11761 gcgagcggca gatcggggca gatgaacttc tcgtcatcaa ctccggcacg atccacgccg
    11821 tgacagccta tccgaatcag gccatcgtcc tgcaggttcc gtacgagatg ctgctgcagt
    11881 acatggagca ttacgatcag ctttactttg ttgtggatga aagaccggcc agtgaggcgg
    11941 aaaaacaaaa actcgcagaa ttcaaagcag ttctccggga gatggacgag gtgtacggtg
    12001 cgcagaaaga agggtatcag ttaaaattcg acagccttct tcttgaagcg ctgtaccgct
    12061 tactggtgga ctatgcggtg cggatcggaa aagaacacat tccgcagaat cagaaggccg
    12121 tacagcagat ccgcgaggtc atgcggtata ttgaggagca ttacgctgaa aagctgacgg
    12181 tggcgcaggt cgcggagcac tttggttata cgccgaatta tctgtcgaag cttctgaaac
    12241 ggtacctcgg tctgacggtc taccagtatc tgtatgaaat ccggctcgac cggatctgcc
    12301 gcgacctgca gcatcccgag gcgcggatca tggaacttct gccgaaacac ggctgcgaaa
    12361 actacaagtc ggtgtcgcgt ctgtttaagg agcggtacgg ctgcagcatg aaggaaatgc
    12421 agaagcggta tgaaaaggaa aaaggagacc gcccgatggg ccagtgggta agaatcagaa
    12481 aagcggaaat gccctaattt tctgccgaaa aaggcactaa acaagaagaa gagagcatgg
    12541 tatagtggaa gcatgaaaca gcggattttc agtaaatgcc gcaaaatatg tgaaaaatgg
    12601 agtgtggaaa atgaaagcaa aacagattac aacgtaccat gtaaaagggg aagcaaaaac
    12661 ctgggaaaaa gcgctggcac cgaaagacga gtccaagagc gtaaaaatga ttgagtcgaa
    12721 cgtcatcaac ctgtatccgg actttgcgtt ccagaccatc gagggcttcg gcggcgccat
    12781 gaccgagagc agcgcatacc tgctgtcccg tatggatgag gaaacacaga atcaggcatt
    12841 acaggatatt ttcggaccgg acggccttca tgcaagattt gtccgcgtgc cgatcgacag
    12901 ctgtgactat tcccttgagg agtatcaggc agtcgcagac ccgatcgcag acccggatct
    12961 ggcaaccttc tcgatcgacc gcgaccgcaa atatgtcctt ccgatgctga aaaaagccat
    13021 cgaaatctcc gcagagccga tcagtgttct gatgagcccg tggtctccgc cgtatcagtg
    13081 gaaaactgca ccgaagatcg caaagaatga tgctgctgtt tacggtgcaa tgggaatgcc
    13141 ggttccggaa gagattccgc agagaaacca cggcggcagc ctgaaaccgg agtattacgg
    13201 aagctgggca aaatacgtcg taaaatatct gcaggcatac ctcgatgagg gaatcccggt
    13261 gacgatgctg agcctgcaga acgagaccgt agcggctacc acctgggaca gctgcgtatg
    13321 gacaccggaa gagtccaaaa cctacctgaa agattatctg tatccggaga tgaaaaaagc
    13381 aggcctgacg gacaagatcg gcatcttcat ctgggatcac aacaaagagc gtatgatcga
    13441 gcacgttctg accgtccttg acgaggagac catggacatg gtagccggta tcgcattcca
    13501 ctggtattcc ggcgaccact tcgaggctgt tgagctgatg aaacagaaat acccggacaa
    13561 aaccttcatg ctttccgagt gctgtgccct tcacatgccg ggcagaatcg gttttggcga
    13621 cggtggtttc ggaccggtga acttccagac accagagtac gtcgattacc ttgacgccgc
    13681 agattatgca cacgatatga tcggaaacct gaacgccggc atgaaccgct ggatcgactg
    13741 gaacttcctc gtagacaaag acggcggccc gcgccacgta ccgatgggat tcacctcagg
    13801 tctgattgtg gatgatgatt tccattaccg caaaccgatc atgtaccact acatcggcca
    13861 tatttccaaa tatattgctc cgggtgcaaa acgcatcggc tggagcaaat acggagcaaa
    13921 cctggatgta acggccgcag tcaatccaga cggaagctac gttgtgatcc tgctgaaccg
    13981 cacaaaagag gattccggct gcttcctgcg tgtcaacgga catatcatgc gtgtcgatct
    14041 tccggcagag acactcagca cggttgtaat cgagaaataa aaagcaaaaa taagaaaatg
    14101 ccttctgtgc agaatggaaa cagtctgcac agaaggcatt tttgtaagat acgatttatt
    14161 tttgatcgga atcggaagaa tctgagtttt tctcttcggt cgaaggcttt gccggtgtat
    14221 acggcgcatc aaccggagac tcttccgtgg tcagctttgt cagcgctttg cgcagccggt
    14281 agcccggaaa gtggaagact tcggcctcgt cgatcttctt tttctgatat gggatcgcct
    14341 gtctcgtttc cggatcaatg tggtccatgt ggaagtaaaa cgcatcgtca tccggcaggt
    14401 gatgtttttc aagacgacgg ttggcgaagt tgcggcagac cgtgcagata atggcacaga
    14461 ccggaacggc gatgatcatg ccaaagacac cccagaagcc gccgccgatc agaatcgaga
    14521 tgatgataag gaagctggac acgccggtcg tctcgccgag gattttcggt cctaagaagt
    14581 ttccgtcaaa ctgctgcagg agaacgacga aaatcaggaa atacagtgcc tgcagagggt
    14641 cgatcagaag gatcagcaga agacacggaa cggcgccgag gaacgggccg aaaaacggaa
    14701 tgatattcgt cacaccgatg attacgctga ccagcagtgc gtacggggtg ccgatcaggc
    14761 tcgtcacaag atagcagatg gcaccgatga tgatcgaatc gatgactttt ccaacgagga
    14821 atccgctgaa ttcctggttc acatactggc agtcgcggat cacggcattt ccgtgatcca
    14881 gaccgagcag cgcgtagact gcttttttca gcttggcaat gtagctttcc ttgttgtaca
    14941 gcatgtagat cgcaacgatc gcgccaagca gaatgttttt gaaaaacgta accatgttga
    15001 cgagctgtcc ggagaggttc agaaggaacg tacggagcat cggcatcagg tcattggtca
    15061 gccagttgga aagctttgcg gagaacgtgg agatgtactg gtctgccgtc tgctgcatct
    15121 ccgggtaatt cgccagcact tttgtggcaa aatcctgaat gttgtttgcg tagttggaga
    15181 agttggagac gatgtttgcg atacttgaaa acagctccgg gatcagggtc gcaatgagtc
    15241 cgtacagcgc gaaaagcatg gagacgtacg tcagaagaat caaaagcgcc cgcacaagct
    15301 ttttgcagcg taccgactgt gtttttccgt gccgggcaaa gagtctggcg atgatccgct
    15361 gttctatgaa gttaatgatc gggctgatca cataggcgag aatcagggct gcaatcaggg
    15421 gggagagaat gttgaggaca ttgtgaaagg cggtccgcac agaagtctgg tggaagagca
    15481 ggtagtaaaa aatcatggac gctgcaagta ccagaaaggc agtaacgccg gcccggacat
    15541 attttcggta acgatccagt ttcataaaaa tgtaaatccc ctttcgaatt ttattaacct
    15601 ctattataca catgcaatcc ccaaaataca accgcaacca cacttgcggt aaaaaaagga
    15661 aactgctata ctggaaaagc agatttagaa aaaggagata acaaatttga agataaaaat
    15721 gccgccaagc gtaaatatgg taatcgattc cctgcatgcg aaaggcttcg aggcgtacgc
    15781 ggtcggaggc tgtgtgcgcg atacgattct tgcgcgggag ccggacgact gggatattac
    15841 aacctctgct tccccacagc aggtaaaaga aatttttgac cgcaccgtgg atacgggact
    15901 ggagcacgga acggtaacag ttctggccgg aagcggcgcg cacgaggtca cgacgtaccg
    15961 catcgacgga gagtatgagg acggccgcca tccgaaagaa gtggtcttta cctccagcct
    16021 cgccgaggat ctgcgccgcc gtgattttac gatcaatgcg atggcataca acaacgaaga
    16081 ggggcttgtg gatctgttcg atggaatcag ggatttacag cgcaaagtca tccgctgtgt
    16141 cggaaatccg accgagcggt tcagtgagga tgcactgcgg atcctgcgtg ccctccgctt
    16201 ttccgcacag ctcggcttca aactcgatcc ggcaacactg gatgccatca tagcactcgc
    16261 gccgacgctt gaaaaaatca gcgtcgagcg gatccgtgtg gaactgaata aactgctgat
    16321 ctcgaagcgg ccggagtaca tggaggccgt ctatgaggcg ggtattacaa aaattgtgct
    16381 gccggagttt gatgaactga tgaaaacgcc gcagaacaat ccgcaccact gctataatgt
    16441 cggtgagcac acgattgccg ctctgcagca cgttccggca gatccgattc tgcgctggac
    16501 gatgctgctc cacgacattg caaaaccggc gtgccgcaca acggatgagg ccggcatcga
    16561 tcattttaaa ggccacgcac cggcggggga gaaaatggcg aaagcgattc tgcgccgtct
    16621 gaaattcgac aacgaaacca tccgccaggt cgcagcattg atccgctggc atgactgccg
    16681 catggctccg gagaaaccgg tgatccgccg tattctgaac aaactcggcc cggagctgtt
    16741 cgaaaaactg atgatcgtgc aggaagccga tacgatggcc aaaagcctgt accagcggga
    16801 ggaaaccctg gaacggatcg ggaaggtaag ggagtgcgtc agagaaattc tcgataacgg
    16861 cgactgcttt acgttgaaaa cgctggccgt ttccggcaaa gatctgatgg aactcggtgt
    16921 cccaaaaggc ccgaaaatcg gcgaatgcct gaacgcggcg ctggaagaag tgatgaaaga
    16981 tccggcgaag aatacaaaag aatacctgac agagtacgta aaaacactgc tgtaaagcca
    17041 aaacggcaaa caagagagcc ggggacagaa caatccgtcc ccggctctct tttgtttttt
    17101 gctggtttta tgcgttcaga tcacgcagca gatccgcata ccagcagaac acatgggcaa
    17161 tgcggtacag ttcgctctcc ttggagacgg tgcgccacag ctctttatag ctgtgcagcc
    17221 accgttcgag atctccggcg agtgcaaaac agtcttcttt ctccggcaga gtgccctcgg
    17281 aaagaaggga agcgcgcagg aacggcagca ggcggttcag cactttcagg ccgtctgcgg
    17341 cgagcaggta cgcatagatc atcggacggt tctccgggcg gacggtgccg gctttctcgt
    17401 acagaacggc gcagagcgcg tcgattgaag catttgctgc atccgcatcg ccttccgcgc
    17461 aggagcggag cagttcggca gcctcttctt tgtcctgatg cagttcgagt gcttcctgta
    17521 ccataacggc agtgcgccac ggatacgcat cctgatggca gagaagatcg agcacggaaa
    17581 cgagttttcc ggaagcatca ccaaattcca gcacggaaat ctggcggttg atttcctcct
    17641 ccggcagaat gtctgcattc caggagaacg ccgcgccgta gatcatgccg atgcgggaga
    17701 agtccgggtg attgatgtgg ccgtaatcgc cccagtcagt gttcagaagt cccatcgcat
    17761 ggcattcgtg gccgtagcgc gccattctgg aaatgttttc atacgcctca tgatacttgt
    17821 tgatgagctg gttccatccc tgtacgcccg ggcatacata cagatgctcg gcaccggaat
    17881 tggcaaggcg ggtcagacgc tccgatgaaa catttgacgc ataatcccag tggaggcaga
    17941 tcaggttctt cggcaggaga tgataaactt ccggctcctg gctgatgaca tcactccaga
    18001 acatcggcgt gcggccggat tcagtgatat agttggcaag ctctgtcaca aactcggtgt
    18061 agatgacggt ggttcccttt tcctcggcga gtgcgcggga agcccctttt ccaaggtcaa
    18121 acgtttcatc cgcgcagaga ttgaaatact tcgaggagaa gagctgcata tattcggaaa
    18181 gaatgtgttt ggcaagggag aggctctccg ggtttgtgat atcgagcgta tggtgcgcct
    18241 ggcggtcata gaaggagaac ggagccgaag cagagccttc cagttcgcac agatgtgcat
    18301 acgatttcgt gcagagcagc ttgtacagat ggccgaagct tgcaagcgac ggaacgagat
    18361 caatgccaag tcccttgcag tagcggtcca gctcgaggat gtcctctgca gtgagcggcg
    18421 tatcgtcgcg ccacatctcg gtcagatcgt cgaagagata gctgtgctcg atgtagagct
    18481 gaagctgatt gattttatag aagctcaagg tgtcggcaag ctgtttcaga taggaaagcg
    18541 tcggaacacg tccgcgggtc gcatcgtggt agaagccgcg gttttccatg gccggtttgt
    18601 cggagatcag gacggttgga agaacagcac ctgcctggcg gatcatctga cggagtgtct
    18661 gcacgccgta gagaacgccg ctttccagag atccgcagat cgtgatgttt tccggtgtga
    18721 cagcgagggt ataagcctca cgggataatt ccggagaggc agtcagatag atcaggtgtg
    18781 atcccaccgg ctgatgacgg cggcagatgg caggacggaa tcccgtctgt gtctcaatct
    18841 cctgctgcag aagctgtgct gcagaaaaaa gctcagccgg gctctcggaa tcgagaaaaa
    18901 tgcggctgtc atagtgaatt gtaaaggtgc cctctccgta ggaaagggaa gatggctgtg
    18961 gtaagaaaat cataaaaacc ctccttaaaa taaaatgatt ttagagaacc ccataacagg
    19021 tgtttttcac cctgtcaagt ttggaatatt ttaccatgcg gtgaaattaa aaacaataaa
    19081 ataagagaaa aagaaaatac ttttaacatt gcttttgaga tgaaagaaaa aacagaaacc
    19141 agtatgcatg gcacagggcg gatacgcata cattgaatcg tgaccccaaa aacgatcaca
    19201 acagggaaag gagtcaggct cttgtatgat tatggattga gtgtgctgtc ccattacggt
    19261 cttacggctt ccggcacaat gcgggtgcgg ggcgctttat tgtgccacac cgaacagggg
    19321 gataagctga tcagcgagtt cgggacggcg gcggaaaaac tggagcagcg gtatcttcta
    19381 cagtgccgca tcacggagtc ggggctcctg cggtgcgatc agattctgcg gaacgaagag
    19441 ggagcgcttg caacgcgcgg ggacgacgga ggaacgtaca ttgtgcggaa ctggtacccg
    19501 ggccgggaat gtgagacggg aaacagtgag gatcttatcc gcgcctcgga tgcgctggcg
    19561 cggctacata cggcagctca tctgccggtg aaaatggaat accggaggga atccctcgtg
    19621 gaggagtgca tccgccacaa tgtggaaatt cgaaagatcc gaaagtattt gcagacaaaa
    19681 aagaagaaaa atgagttcga aaaactgctg ctggcaagcg ccggtccgta tctggaacag
    19741 ggagagcggg cggccgcgga aatgaaagct tcttcctatg aaaaactgcg gcagcgggcg
    19801 gacgaagaag gcgccgtctg ccacggggaa ttcagccagc acaatattct gctggaaaac
    19861 ggcagaggcg aaacggcggt caatttcgac aagtggaact tcgatctcca ggtggcagat
    19921 cttggctatt ttatgcggaa aattctggaa aaacacggct ggagcgagcg cgcggcagaa
    19981 cggatcttaa acgcctacag cgccagacgg ccgctcgatg agggggaaat cgagacactc
    20041 cgcctctatc tttcctatcc gtggaaatac tggaaactgg caaaccgcta ctacggaagc
    20101 agaaaatcgt ggatatccgg gcggaatacg gagaaactaa aaacactcaa tgcgcagcag
    20161 gcagactggc tgcggtttgt taagggatcc ttcttttaac cgtgcggtgg aaaagagtct
    20221 gttttgggca gaaatcgcca taaaaccgct ggtatggggg acttttttct tgacaagaga
    20281 gacgcattag tatataaata tatacagcta cgcgaaaaag ccggtcaacg taacagactg
    20341 atcatccgga agaaaaacag ccggcagggc aggtcgtgtg tatcagcggg cccgcgtacc
    20401 atgtaaaaaa caaatgtaag atacaggagg agtaaaactc atgaacaaaa cagaattagt
    20461 tgcagctatg gctgagcaga caaatttatc caaaaaagat gcagaggctg ctttaaaagc
    20521 atttatcgat gtagtatctg aagaattaaa gaaaggtgag aaagttcagc tggtaggctt
    20581 tggtaccttc gaagtatccg aaagagcagc aagagaagga agaaatccgc agactggcga
    20641 gaccatggaa atcaaagctt ccaaaactcc gaaattcaaa gcaggaaaag ctttaaagga
    20701 catgatgaac taagtaaaaa tctatgcgct tagataaatt tttaaaagta tcaagactga
    20761 tcaagcggcg taccgtagcc aatgaagcct gtgatgcagg ccgtgtcctc gtcaacgaca
    20821 gaccggcaaa agcatccgca caggtcaagg caggagatgt actggaaatt cagttcggaa
    20881 gtaaatccgt cagggtagaa gtgctgaatg tgcaggaaac cgtgaaaaaa gaagaagcac
    20941 aggagcttta ccgctactta taagaagtgc ggcaggattc atgtgcagcg aataaaaaga
    21001 gaaaaccttc atataatggg tgtatagacc attgtatgga ggttttttgc atgggggaga
    21061 tgaacgaaca ggtgcaccgc cttttgctgg aacagcggaa aacactttct cttggcggcg
    21121 tgcaggatgt gaaaagcttt gatgagaacg aagttctgct cgtgaccgtc tgcggtgttc
    21181 ttaccatccg cggaaaagag ctgcatgtgg gccgcctgga gctggaaaag ggagaggcgg
    21241 atatcgaagg cagcatcgac agcctggtct atacggaaca tgggccgctg cagaagaagg
    21301 ggtctgcgct tgcgcggctg ttccggtaaa aatgagtgcg tatgtcaccc gggagctgat
    21361 tctgctcgcc tgttccatct ggcagggggc agtcctgggg ttctggtacg atctgatcaa
    21421 gacagcgcgg aaactgatcc gccacagcca tgcggcggta tctgcagagg acctctgcta
    21481 ctggattgcg gcagcgctgt atctgtttgt acggatttac ggagagaaca gcggcattct
    21541 ccgtgggtat ctgtttggcg gcatccttgg gggcgctgca ttgtaccacg catcgtttgg
    21601 acgctttctt ccggaacttc tggcaaaggg aatcaaaaaa gcagggcgaa tcctcgcttt
    21661 tccaggaaaa tggctggtaa aaagtatgaa aaaacggaga aaaaggttga aatttcatgg
    21721 gaagaacggt aagatgtaac tacacatgta agaacaaaag aaacaagtct gcggacgaaa
    21781 aacaacagat agtttggctg cagatggttg gaaaatagaa agcaagggta aagaaaagaa
    21841 tgaaagaaag ggaagaaaag aattctggcc gaaagaaaaa agtcagaaag aagagccgta
    21901 caacagagtt ctcaattacc atagtcctgt gctgcctggt tgcagtcctt accgtaaagg
    21961 gaatcagcct tcagcagaag gtcaatgcaa atgcatcccg tctttccagt ctggacagcc
    22021 agattgaaga ggagaagcag cgcaccgatg atattaccgc cttgaaagaa tacatgcaga
    22081 ccgatgagta catagaaaaa acggcaagag aaaaacttgg catggtaaag gataatgaga
    22141 ttttatttaa agaatcccag tcgaaataaa caaaacagat aaagaaaggg catgatttcg
    22201 cagtcccctt tgaagtccag aacaaagaga ggaggagcgg aacatgccgg agtggatcat
    22261 tgccatgttc ctgatcggcg cattgctgtc agcaggggac atggctaaaa tttttttgga
    22321 agccagaaga agccgccggg aagcggccgt ctatgacaat catccgcaga aactgcagat
    22381 ggagcactac gccgattcgt tccgcgttct ggcagagagc ttttaccaga tgccgtcaaa
    22441 aagtgtgatg ccggagacgg gccgggtgga caagattctg gaaaaagaac agcaggaagt
    22501 ctgtagccgc tgtgcgaagg cgtcgtggtg ctgggagcag tacggaaacc tgacgcgcga
    22561 gcggtgccag gaacttcttc agacaatcgc cgacggggat gaggacgaga tcagccgtgc
    22621 aaaaggcgaa tggaacgcct cctgcctgaa cggctcccgc tttctggaac tgttctggaa
    22681 ccgctaccag caggaacgcc agacggcgct ctggtcgggg cgtgtggcgg agagccgccg
    22741 cgtggtggca gaacagttaa gtgaagtcgc cgggattatg gaccgcgccg cgtgtgatct
    22801 gtacagcctc aacagtctgc cggacgagct ctcggaaaaa atctgccgcc agatgaagaa
    22861 aaacggggct ttcgtccaga aaatctggct ccaggagcgg ccgaaagagc atttacagat
    22921 ctatatgacg gtcaaagcgg gccgtcactg ccggatcacg cttcgccaga cggcggaact
    22981 gatcggttcc ctgtgcggaa tcccgatggt tgcggtacgc gacggggcac aggtcttaag
    23041 cggagaatac cagacggtgc tgtttcagga ggatgtgaaa ttccaggttc tctacggtgt
    23101 gggacgcatc accaaggagc aggagagtat ctccggggac aattacagtg tgctctgcga
    23161 aaacggccag tttgtcctct gcctgtcgga cggcatgggt tccgggatcg aggcaaacag
    23221 ggagagcgag acggtggtgg agctgtttga acagttcctg cgcgccggat tcccgcggat
    23281 catggcggca cggatgatca attcgatgct tctgctgcag ccaaaggaag ggatgttttc
    23341 cacgtttgat gttgcttcga ttaatctcta cacaggcgtc tgcagctttt tgaaaggagg
    23401 tgcatcgccg acgttcctta ggcgggatac gtgggtggag gttgtggagt cgaccagtct
    23461 tgccgcaggt ctcgtttccc agacggattt tgataccaca acgaaaaagc tgtacgacgg
    23521 tgattacctt gtgatgatga cggacggagt gctggacgcg ctgcccgggg acaggacgga
    23581 gcagatgaag cagcttcttc tcgaagtgaa aaacagcgag ccgcgggaat ttgcccggga
    23641 gcttttggaa cgcgtgctgc gcctcggcgg ctgccgcgca cgggatgata tgacggttct
    23701 ggtcgccaga atctggaaaa aataggagta aaaacgaagg atgcgagaga aagtaagagc
    23761 ctttatggaa cagtatcata tggtaacggc aggcgaacgc gtgctgctcg gcctctccgg
    23821 cggcgcggat tccgtctgcc tgtttcatct gctgcgggaa ctgcaggagc cgcttggctt
    23881 ttcgctgctg gctgtccacg tcaaccataa tctgcgcggc gcggaagccg ggcgggatgc
    23941 ggcatttgca gaaaatctgt gccgatacgc cgtggttcac ggatattcag caagccc
//
LOCUS       DAOMUF010000012        22396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_367_length_22396_cov_3.264312, whole genome shotgun sequence.
ACCESSION   DAOMUF010000012 DAOMUF010000000
VERSION     DAOMUF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 22396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22396
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_367_length_22396_cov_3.264312"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLN77_01795"
     CDS             complement(<1..561)
                     /locus_tag="PLN77_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRM43048.1"
                     /translation="MIVKILIAVLIFGITVIIHELGHFLLAKANGITVTEFSIGFGPT
                     VVKTEKGGTIYSLKLLPFGGACQMLGEDGEEEGEGTFNSKSVWARISVVAAGPVFNMI
                     LAFFCAIFVISYAGSSPARVTEVADGSPEAEAGLEAGDIITSYEGRHISIGKELNSVM
                     TVRGVPTDEITLEVKKADGEKKTITYE"
     gene            complement(568..1722)
                     /locus_tag="PLN77_01800"
     CDS             complement(568..1722)
                     /locus_tag="PLN77_01800"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRM43049.1"
                     /translation="MKKIAILGSTGSIGTQTLDVVRANGDLEVVGISAGSNITLLEQQ
                     IREFHPKFAAVGDEAKAAELRDRVKDLPVKIGSGMDGMIELATMPESEILVTAIVGMI
                     GIRPTVAAMKAGKDIALANKETLVTAGHIIMPLAEECGVQILPVDSEHSAIFQCLHGE
                     NRKEIEKLLITASGGPFRGKTRAELENVTVEQALRHPNWSMGHKITIDSATLVNKGLE
                     VMEAKWLFGVDLDHIQVVVQPKSIIHSMVEFKDGAVMAQLGTPDMKLPIQYALYYPER
                     RFLAGDRLDFAALTQITFEKPDMDTFLGLPMAMQASRTGGSMPTVFNAANERAVALFL
                     AKKIRFLEIYDVIAGAMEAHKTIADPTLEQILAAEQETYEWIANRYKMGE"
     gene            complement(1773..2564)
                     /locus_tag="PLN77_01805"
     CDS             complement(1773..2564)
                     /locus_tag="PLN77_01805"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRM43050.1"
                     /translation="MFKTRLLSGIVLVLVALLTICSGGTVLYMTVLCLSLIGAGELFR
                     VMKVREDKWNLLEITGYAGVVIFYLSLLLHDPMYQMLALILDLVLLMAVYVFTYPKYN
                     ANQVMPAFFAIVYVGVMLSYVYQTRMLDGGKFHVWLIFLCSWGCDTCAYCVGVLFGKH
                     KMAPVLSPKKSVEGAVGGVVGAILLGIIYAAATKGGMVEYAIICGVGALISMVGDLAA
                     SAIKRNQGIKDYGKLIPGHGGILDRFDSVIFTAPIIYFLSVLLIH"
     gene            complement(2570..3307)
                     /gene="uppS"
                     /locus_tag="PLN77_01810"
     CDS             complement(2570..3307)
                     /gene="uppS"
                     /locus_tag="PLN77_01810"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRM43051.1"
                     /translation="MDNELKVPNHIAIILDGNGRWAKKRNMPRGYGHIVGCDNLEKMC
                     DVIKDMGVKYLTVYAFSTENWKRSRAEVEGLLKLFRQYLKRCIKKAHKNQMRVKIIGD
                     PSAFPQDIQDSIHELEECSKDYDHMYFQIAMNYGSRDEILRGMTRMIEDVKNGTLAEN
                     AFDENGKLTEQCFESYLDTAGIPDPDLLIRTSGEQRLSNYLMWQLAYTEFYFTEVPWP
                     DFHEEELRKAIEQYNNRDRRYGGVKEE"
     gene            complement(3375..3926)
                     /gene="frr"
                     /locus_tag="PLN77_01815"
     CDS             complement(3375..3926)
                     /gene="frr"
                     /locus_tag="PLN77_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRM43052.1"
                     /translation="MDERVKPFEVKMGKSIASLDSEFGTIRAGRANPHVLDRITVDYY
                     GTPTPLQQVANISVPEARMIQIQPWEASLVKEIEKAIMMSDLGINPSDDGRVIRLVFP
                     ELTEDRRKELVKEVKKKAEGAKVAIRNIRRDANDAFKKLAKEDVSEDEIKELEEQTQK
                     LTDKYIKEVDQMVDVKSKEIMTV"
     gene            complement(4104..4802)
                     /gene="pyrH"
                     /locus_tag="PLN77_01820"
     CDS             complement(4104..4802)
                     /gene="pyrH"
                     /locus_tag="PLN77_01820"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRM43053.1"
                     /translation="MKRRVLLKLSGEALAGEKHTGFDEPTVTKVAKQVKTLVDQGIEV
                     GIVIGGGNFWRGRSSENIDRTKADQIGMLATVMNCIYVSEIFRSQGMMTAVLTPFPCG
                     EFTKGFSKDRANKYFQHNMVVFFAGGTGHPYFSTDTATVLRAIEIEADEILLAKAVDG
                     VYDSDPKTNPDAKKFDEISIQEVIEKDLKVVDMTASILCKENKMPMLVFGLNEENSIV
                     NAANGKINGTRVTV"
     gene            complement(5208..6527)
                     /locus_tag="PLN77_01825"
     CDS             complement(5208..6527)
                     /locus_tag="PLN77_01825"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRM43054.1"
                     /translation="MKKVVKFGGSSLASAEQFKKVGDIIRADEGRRYVVPSAPGKRNS
                     ADTKVTDMLYDCYRTAVAGRDFKKKLQNIKARYQEIIDGLNLSLDLSPEFEKIAENFA
                     AAAGEDYAASRGEYLNGIVMANYLGYTYIDSAEVICFDENGNFLADKTDEVLSERLKD
                     LDNAVVPGFYGAKPDGSVKTFSRGGSDITGSIVAKAVHADIYENWTDVSGFLVTDPRI
                     VKDPEVISTITYRELRELSYMGATVLHEDSIFPLRQEGIPIHVLNTNAPQDPGTMIVE
                     NTCSKPKFTITGIAGKKGFASITVEKSMMNTEIGFGRKVLGVFEDNNLSFEHMPSGID
                     TMTVFVHQNEFAEKEQQVIAGLHRAVQPDSIDLESDLALIAVVGRGMRRTRGTAGRIF
                     SALAHAHVNVKMIDQGSSELNIIIGVENRDFETAIKAIYDIFVVAQI"
     gene            complement(6662..7981)
                     /locus_tag="PLN77_01830"
     CDS             complement(6662..7981)
                     /locus_tag="PLN77_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRM43055.1"
                     /translation="MKNRLHVKAPGNWVNDPNGMIYYKGQYHLFYQHFPYAPRWGTMH
                     WGHAVSPDLIHWEHVGVALFPSLSEDQNGCFSGSAVEENGVMHLFYTGVHYNQVNPKD
                     IHQCLDQDFTSAQLHLTSKDGMHFDVFGDKEVVIPALTDPEIGDNTHTRDPKVWKGSD
                     AWYMVLGSTTKDHKGEALFYKSEDLSHWHLAGQATTKQKLGWMWECPDLFNVNGRSVF
                     VFSPMELIEDTDGYADRAICMLPEFDEKTCTMKFPENWQLLDCGGDLYAPQTMTDADG
                     NRISIAWLRMPEPVDGIWQGMFCMPRVVDVQNDHIYFRVLPAIRDGFCKKTDSLVSAC
                     GECAMIRAELKEGESLEIGGYKIWRQSGKVCADRTDVYPSGAKGWLHAETPQLKEGDL
                     LEIFVDPNMIEIYANNGEYVLSQAVYGLGETFSYEMEKKPEIYLWNE"
     gene            complement(8099..8959)
                     /locus_tag="PLN77_01835"
     CDS             complement(8099..8959)
                     /locus_tag="PLN77_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM43056.1"
                     /translation="MNQKKIGGTKKKVTDALAMLLMIIVFCAFMFPFVMVIINSLKQK
                     RDIIKSPFSWLFTIKGLSFDNFVKAFNQMNFLNAFKNSLVVTVTATVLVTVLAAMLAY
                     YIVRNVNVFSNIIFALMVASMIIPFQAIMIPLVSVYGGTLNILNHRLTLIFMHTGFSM
                     AMSVFMFHGFIRGSIPLALEEAAYIDGCTHTQTFFRIVFPLLKPIISTMVIMNAMAFW
                     NDFLLPSLVLTDKKLLTLPLSTYSFYGTYSADYGTIMAGLLLCVIPILILYVALQKQI
                     INGVVAGAVK"
     gene            complement(8959..9831)
                     /locus_tag="PLN77_01840"
     CDS             complement(8959..9831)
                     /locus_tag="PLN77_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRM43057.1"
                     /translation="MKSSNKGKDFCVFALPGLFCFLAVVMVPFIYGIYLTLTDWNGVS
                     SVKNFVGLSNFAAVMRDAQFWKSLLLTFKYVIAVVVLVNVIAFLLAYILTRGIKGQNF
                     FRAGFFTPNLIGGIVLGYIWQFVFSRVFVSIGETTGWSLFEISWLSTPNKAFAALVLV
                     SVWQLAGYMILIYVAGFMGLSEDVLEAASIDGASGWVKMKNIILPLMMSSVTICLFLT
                     LSRAFMVYDVNLSLTGGAPYGTTEMAAMHVYEKAFTSRQFGVGQAEAFILFIIVACIS
                     GIQVYTTKKREVEA"
     gene            complement(9859..11232)
                     /locus_tag="PLN77_01845"
     CDS             complement(9859..11232)
                     /locus_tag="PLN77_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43058.1"
                     /translation="MRKKFSARAAALGMSGVLLTSALTGCTFGFASDPDDPNAVKEEV
                     PIDMTTDTFQYDTSLSGTSIVIMNTKAEIQTALEKIAKVFEEKAGVHIEIMPVTDGDS
                     PYTKVVSLYNSGNPPTLSILDTTDVIALAEEKAADLSNEAWTGEAEEYLTKINGKVYS
                     FPLCIEGRGIIYNKSVIEDTLGTEFDPSDIKTQDDFAALLQSLRDNGMKNPISMAKED
                     WSLGAHQLQYIYETYNGTSDGAAEVINELKDGTLYLPDYTRMNEFLNTFDLLKEYNVA
                     KGDPLGADYDEMAIDLADGKTAFWFNGNWAWPNLEEAGAETTDEYGFLPYFLNNDPDD
                     FVNSKIQASPSKQIMLDDIVATDEQKAAAKEFLNWLVYSEIGQQMVVKTCSLIPPFKN
                     NPNEPSDPLSRDIYEKVQNGGAFNASAIVPNDHWSVLGAAMQKYLAGRSDREELIDSI
                     QAYWDEQ"
     gene            11545..12573
                     /locus_tag="PLN77_01850"
     CDS             11545..12573
                     /locus_tag="PLN77_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRM43059.1"
                     /translation="MARKKNVTFNDIAQYTHFSKTTISRYFNNPDSLTPENQQIISDA
                     LDKLNYKENKVAKILANGQTEFVGVLIPSLSMNYYSEMLNQILASYEKYGYKFLVFAG
                     NGHDETEHRYIQELMSYKIEGLIVLSHTLSSRELSDLQIPVVAIEREDQFVSSVNTDN
                     YLGAYEATSLLIHNHCDVLIHLNSPTKENVPAYRRLAGFQDACVKAGVPHKVIIRDFG
                     LSYQEAAAPLQEIFDNIEQKYAGQKKGIFLSNDDHANILLNLLIRKYHTLPDDYELIG
                     FDGAPISEQAIYPISTVGQQIDQIADEAVSLLVSQMQERKKRHPVPLAEPVHKTVPPL
                     LIRRQTTT"
     gene            complement(12688..13383)
                     /locus_tag="PLN77_01855"
     CDS             complement(12688..13383)
                     /locus_tag="PLN77_01855"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HRM43060.1"
                     /translation="MLEELKKRVYEANMLLPKYGLVTFTWGNVSEIDRESGLFVIKPS
                     GVDYDLLTPDDMVVMDLNGNKVEGRYRPSSDTPTHLELYKAFPEIGGIVHTHSSYATS
                     WAQAGRSIPCYGTTHADYIYGEVPCLRCLTKEEIDEAYEENTGHLIVNEFKRMGKDVK
                     AVPAVLCKNHGPFTWGKDAHEAVHNAVVLEEVAKMAYRAETINPRIQPAPQELQDKHY
                     YRKHGANAYYGQN"
     gene            complement(13418..15016)
                     /locus_tag="PLN77_01860"
     CDS             complement(13418..15016)
                     /locus_tag="PLN77_01860"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRM43061.1"
                     /translation="MGMDAKTAILEQKTALGIEFGSTRIKAVLIGADNAPIASGDHEW
                     ENRYDNGVWTYTLEDIWTGLQDAYTKMAADVKEKYDITLTRVGAIGFSAMMHGYMAFD
                     KAGNLLVPFRTWRNNITEEASEKLTDLFGFHIPQRWTIAHLYQAILNGEPHVADIDYV
                     TTLAGYIQWKMTGERVVGIGEASGIFPIDSETNTYFADMIAKFDEAVADKAYSWKALD
                     VLPHVLTAGDNAGVLTKEGAALLDMSGNLEAGIPLCPPEGDAGTGMAATNSVRVRTGN
                     VSAGTSVFAMIVLEKNLSKVYPEIDMVTTPSGHPVAMVHCQNCTSDLNAWVNLFREFA
                     QTFGMEISTNDLFGKLYNKALEGDADCGGLLAYNYFSGEHVTGFNEGRPVFARTPDAK
                     FNLANFMRVNLFTSLGALKVGLDILMKEEHVQVDQILGHGGLFKTKGVGQKILAGAID
                     APVSVMETAGEGGAWGIALLASYMINKEENETLEDYLDAKVFAGNAGTKMDPDPADVA
                     GFEVFTERYKKGLPIERAAVESLK"
     gene            complement(15043..16161)
                     /locus_tag="PLN77_01865"
     CDS             complement(15043..16161)
                     /locus_tag="PLN77_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM43062.1"
                     /translation="MEPTTGKAKYYTLMEALKEQILSGTIKPGQKLPSENELTREYAL
                     SRHTVRKALALLENEGYITARHGKGTFCSERVIQRHNSKNIAVTTTYLSDYIFPRLIQ
                     GMDRVLSENGYSIILKNTCNSRSKEEKVLKELLDKPIDGLIIEPSKSQILCRHINLYE
                     MLDKYQIPYVFIQGCYPQMADKPCILMDDCKGGYLLTRHLLETGRRNIIGIFKADDSQ
                     GAQRHKGYVQALQESGILYDPENVIWFHTEDRKTKPSLMLSMLLDSKKSVDAAVCYND
                     QIALAVISMLEEKGISVPEDIAVTGYDNSLIGQSSPIGITTIAHPQEKLGEMAAELIL
                     EKIRKVPEEESSVKRLISPELIVRESTAGKNLSTETKK"
     assembly_gap    16329..16368
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16542..17159)
                     /locus_tag="PLN77_01870"
     CDS             complement(16542..17159)
                     /locus_tag="PLN77_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HRM43063.1"
                     /translation="MKAWQHFKTITTHKMWVMRYCFKIGLYWQGLTHDLSKYSPTEFL
                     VGMKYYQGDRSPNNAEREDTGMSKSWMHHKGRNKHHFEYWIDYGINCDTIIKGVPMPR
                     RYVAEMIMDRISASRVYLGDAYTDQAPYQYLKKGIGHLWFVHPETLSQLEFLLRMLSE
                     RGEDDTLYYIRYHFLKGDPVPRMHRSAECTAYEEAIRKKVFPSTH"
     gene            complement(17137..19032)
                     /locus_tag="PLN77_01875"
     CDS             complement(17137..19032)
                     /locus_tag="PLN77_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM43064.1"
                     /translation="MIQTKNRGKYRILPGNPEKMGASATKDGYNFAVEVSDGKKAELL
                     LYRKGHQKPFLTVPLPEEERTGVISSVEITGLTVGKFSYAYRMDEAFCLDPYAGAVEG
                     RETFGAPMEKEEGACCCLLPEYPVMRTTSLHRPYEETILYKLHVRGFTKDGSSRVKNK
                     GTFRGVIEKIPYLKELGINAVELMPSYEFFEWTSLTEPAYVYPAPAEEKRVNYWGYGT
                     KALYFAPKASYAASSDAVAEFAEMVDALHENGIECLMEFCFAPGTPSGFVLQVLHHWQ
                     LRYQIDGFHLVGDASLAEEASKDALLRKTKLIFLGFDGARIYQGKRPWFRNLGEHNQG
                     YQYHIRRFLKGDEGSLSDFTYYLRRSPETHGVINYLADHDGFTLYDSVSYEQKHNLDN
                     GEDNMDGSNENLTWNCGAEGVTRKPAVRALRLRQLKNAVLMLMTSQGTPLIYGGDEFG
                     NSQKGNNNAWCQDNKTGWIDWKAAARNPEFAAFVKAAIAFRKAHPALHGEREPRLIDY
                     KSYGCPDMSFHSQRAWFSQMENTCRFIGVMYCGSYFQDKDGNKDDDLYIAYNMHWNPH
                     ELALPSLPEQKVWYLTADTNTPEVFLTEENQKKVSAKTVIVPPRSIIILTGKQEKRQN
                     DESMAAL"
     gene            complement(19129..19884)
                     /locus_tag="PLN77_01880"
     CDS             complement(19129..19884)
                     /locus_tag="PLN77_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRM43065.1"
                     /translation="MKKMISWNVNGLRACMKKGFQEYFEAADADIFCLQETKLQEGQI
                     DLELPGYHQYWNYAEKKGYSGTALFTKEEPISVRYGIGIEEHDTEGRVITAEFPEYFV
                     VTVYTPNSQDELKRLAYRMEWEDAFLSYLKGLEQEKPVIFCGDLNVAATEMDIKNAKA
                     NEKNAGFTKEEREKFAVIKEKGFVDSFRMLYPDTVTYSWWSYRFQARQRNAGWRLDYF
                     MVSESLKDAVADAKIHTEIFGSDHCPVELDLEV"
     gene            complement(19966..21426)
                     /locus_tag="PLN77_01885"
     CDS             complement(19966..21426)
                     /locus_tag="PLN77_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM43066.1"
                     /translation="MDQNRMPLVEALQQFREEAPAYFRIPGHRFERGLDEALNGASLA
                     AYDLSEAEGLDDLHHAEGVIAEAQRLAAETWKAKKTFFLVNGTTCGNEAMVLSSVKEG
                     EKIIVPRNVHKSVLMGLILCGATPVYVMPEYSTKWQMWGGVTPETIEKAFAETPDAKA
                     VLLVSPTYYGLCSDLQAIAEICHCHNAALLVDEAHGSHLYFSEQLPLGALESGADACA
                     QSIHKTAGSFTQSSFLSLGSGRLDEARVAANLQMVQSTSPSYLLMASLDAARRGMALH
                     GKERMERALELGQRARQELAKIKGIEVLGTEMEGTCGVWKIDPTRLVFSARELGISGY
                     DLQVRLYEEYRVSTELADEENVVCVITWANSEEDITRLIDALAGISAAESGKTTGRTK
                     FTEKQWLFRSLPEMVKTPREAYFAEKEAVPFAEAEGRIAAEMAAPYPPGIPLICPGER
                     YSREMLELMRQYKADGCEFHGPSDASLEVLYVLKEE"
     gene            complement(21540..>22396)
                     /locus_tag="PLN77_01890"
     CDS             complement(21540..>22396)
                     /locus_tag="PLN77_01890"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRM43067.1"
                     /translation="MKAYRTAEGKIQLFRPEMNARRMIKSNERLCMPGVPEEMFVEAV
                     KALVKAQEDWVPSEPGTSLYIRPFMFATEASLGVHMAKSYKFVIITTPVGAYYAEGIN
                     PVKILVEDEYVRAVKGGTGFTKCGGNYASSIAGQVKAEKMGCTQVLWLDGVHRKYVEE
                     VGTMNIMFKIDGEIYTAPLEGTVLPGVTRDSLIHILRDWGYKVNETHLSIDDLMKAGH
                     DGTLEEAFGTGTAAVVSPVGEFVYKDDSVIVNDFKIGELTQKLYDTLTGIQWGKLEDK
                     YGWTTPVE"
BASE COUNT         4822 a         5859 c         5456 g         6219 t
ORIGIN      
        1 ttcatatgtg atcgttttct tttccccgtc ggccttttta acctccagcg taatctcatc
       61 cgtcggcaca ccccgtaccg tcatcacaga gttcagctct tttccgatgg aaatatgtct
      121 tccctcatag gaagtgatga tatctcccgc ttccaggcct gcctctgcct ccggcgaacc
      181 gtctgccact tctgtgacgc gcgccgggga acttcccgcg tagctgatca caaagattgc
      241 gcagaaaaat gccagaatca tgttgaacac cggtcccgcc gcaacgacgg aaattcttgc
      301 ccacacggat ttgctgttaa aggttccttc cccctcctct tcaccgtcct cgccgagcat
      361 ctggcacgca ccgccgaaag gcagaagttt taaggaatag atcgttcctc ctttttctgt
      421 ctttaccacc gtcgggccaa agccgatcga aaattccgtc acggtaatac cattcgcttt
      481 cgccagaaga aagtgcccca gctcatggat aatgacggtg attccgaaaa tgagtacggc
      541 tatcaggatt tttacaatca tgctgtctta ttctcccatc ttatatctgt tcgcaatcca
      601 ttcatacgtc tcctgctccg ccgcgagaat ctgctccagc gtcggatcgg caatcgtttt
      661 gtgtgcttcc attgcaccgg cgatcacgtc gtaaatctcg aggaaacgga tcttcttcgc
      721 gaggaacagt gcaacggcgc gttcatttgc cgcattgaag accgtcggca tggagccgcc
      781 cgtccgggac gcctgcattg ccatcggaag gccgaggaag gtatccatat ccggtttttc
      841 aaacgtgatc tgtgtcagcg ccgcaaagtc gaggcggtcg cctgcaagga agcggcgctc
      901 cgggtaatac agtgcgtact ggatcggcag tttcatatcc ggtgtgccaa gctgtgccat
      961 gacagcgccg tctttaaatt ctaccatgga atggataatg ctctttggct gtaccacaac
     1021 ctggatgtgg tcgagatcaa caccgaacag ccattttgct tccatgactt ccagtccttt
     1081 gttaacgagt gtcgcggaat caatcgtgat cttgtgcccc atcgaccagt tcggatgacg
     1141 gagcgcctgc tctaccgtta cgttttcgag ctccgctctt gtttttccgc ggaacggacc
     1201 gccggatgca gtaatcagaa gtttttcaat ttcttttctg ttttccccgt gcaggcactg
     1261 gaaaatcgcc gagtgctcgc tgtccaccgg aaggatctgc acaccgcatt cctctgccag
     1321 cggcataata atgtgtcccg ccgttaccag tgtctctttg tttgccagtg caatgtcttt
     1381 tcctgccttc atcgccgcaa ccgtcgggcg gatgccgatc atgccgacga tcgctgtcac
     1441 aaggatttcg ctctccggca tcgtggcaag ctcgatcatg ccgtccatgc cgctgccgat
     1501 ttttacagga agatccttca ctctgtcacg gagttctgcc gctttcgcct catcgccgac
     1561 tgccgcaaac ttcggatgaa attcccggat ctgctgctcg agcagtgtaa tattacttcc
     1621 ggcgctgatg ccgaccacct caagatcgcc gttggcacgc acgacatcga gggtctgggt
     1681 tccgatcgaa ccggttgaac caagaattgc tattttcttc atattccagt ttctcccatc
     1741 tgcttcccgt ttatgctgta ggggaagctt ttttaatgaa tcagaagcac cgacaggaaa
     1801 tagatgatcg gtgcggtaaa gatcacgctg tcgaagcggt cgagaatacc gccgtggccc
     1861 ggaatcagct ttccgtaatc cttgatgccc tgatttcgtt tgattgcgga tgccgcaagg
     1921 tctccgacca tggagatcag cgcgccgaca ccgcagatga tggcgtattc caccatgccg
     1981 ccctttgtcg cagccgcata gatgatgccg agcagaatcg ctccgacgac accgccgaca
     2041 gctccttcta cggatttttt cgggcttaaa accggcgcca ttttatgttt tccaaagagt
     2101 acgccgacac agtatgcaca cgtatcgcag ccccaggagc acaggaaaat cagccataca
     2161 tggaattttc cgccgtccag catacgggtc tgatatacat aggaaagcat tacgccgaca
     2221 tagacaatcg caaagaacgc cggcatcacc tgattggcgt tgtatttcgg ataagtaaat
     2281 acgtatactg ccatcagaag cacgagatcc aggatcagcg cgagcatctg gtacatcggg
     2341 tcatgaagca gcagggacag atagaaaatc acaacgcccg catagcccgt aatttccaac
     2401 agattccatt tatcttctct taccttcatc acgcggaaca gctctcctgc gccgatcagc
     2461 gacaggcaca gaaccgtcat atagagcacg gttccgccgc tgcagatcgt aagcagcgcc
     2521 acaaggacga gcacgatgcc gcttaacagt cttgtcttaa acatcttttt tattcctcct
     2581 tcacgccgcc gtaacgccgg tctctgttat tatattgttc gatggctttt ctcaattcct
     2641 cttcgtggaa atccggccac ggaacctcgg taaagtaaaa ttccgtgtag gccagctgcc
     2701 acatcaggta gttggaaagg cgctgctcgc cgcttgtacg gatcagaaga tctggatccg
     2761 ggattccggc ggtgtcgagg tagctctcaa aacactgctc cgtcagtttt ccgttctcat
     2821 caaacgcatt ctctgcgaga gttccgtttt tcacatcctc gatcatgcgg gtcatgccgc
     2881 gcagaatctc atcgcgggaa ccgtagttca ttgcgatctg gaagtacatg tgatcgtaat
     2941 ctttggagca ctcctccagt tcatgaatgc tgtcctgaat atcctgcggg aatgcgctcg
     3001 gatctccgat gattttgacg cgcatctgat ttttatgtgc ttttttgatg catcgtttca
     3061 gatactggcg gaacagcttt aagaggccct ccacctcggc gcgggagcgt ttccagttct
     3121 cggttgagaa cgcatagacg gtcagatatt tgacacccat gtctttgatc acgtcacaca
     3181 ttttttccag attgtcgcat ccgacgatat ggccgtagcc gcgcggcata ttgcgttttt
     3241 tcgcccatct gccgtttccg tccaggatga tggcgatgtg attcggcact tttaattcat
     3301 tatccatact tttttcctcc gtaaacgata acaggctgcc tgcaaatgac aggcagcccc
     3361 tcgttttctt tgtattacac agtcataatt tctttggatt ttacgtccac catctgatct
     3421 acttctttga tatatttgtc tgttaatttc tgggtctgct cctcgagctc tttgatttcg
     3481 tcctcggaaa cgtcctcttt ggcaagtttc ttgaacgcat cattggcatc gcggcggatg
     3541 ttgcggatgg ctacttttgc gccctctgct tttttcttta cctcttttac caactcttta
     3601 cgacggtcct cggtaagctc cgggaatacc agacggatga ctcttccgtc atcggacggg
     3661 ttgatgccaa ggtcagacat catgatcgct ttctcgatct cttttaccag ggatgcttcc
     3721 cacggctgga tctggatcat acgtgcttcc ggaacagaga tgtttgccac ctgctgcagc
     3781 ggagtcggtg ttccatagta atctacagta atgcggtcca gtacgtgtgg gtttgcacgg
     3841 cctgcgcgga tggttccgaa ttcggaatcg aggcttgcaa tggatttgcc catttttacc
     3901 tcaaacggtt ttactctctc gtcgatctga tttgccatat taaatttgtc ctcctgctat
     3961 ctcttatacg gtcacagttc tgcgaactgt gacattcgca gctctcattt aaagttttgc
     4021 ttcgcaaaag gagagctgct cacacctgaa tcattttctt acgcagtaaa acagcaagtg
     4081 ttttactgct gaatttttat taattatacc gttactcttg tgccgttaat ttttccgttt
     4141 gcagcattta caatgctgtt ctcttcattt aaaccaaata ccagcatcgg cattttgttt
     4201 tccttgcaaa ggatggatgc ggtcatatct accactttca gatccttttc aatgacttcc
     4261 tgaatggaaa tctcgtcaaa tttctttgca tccgggtttg ttttcggatc gctgtcgtag
     4321 acaccgtcta ctgcttttgc aagcagaatc tcgtctgcct cgatctcgat cgcgcgaagt
     4381 acggttgccg tatcggttga gaaatatgga tggcctgtac cgccggcaaa gaatacaacc
     4441 atgttgtgct ggaaatattt gtttgcacgg tctttggaaa atccttttgt gaactctccg
     4501 catgggaatg gagtcagaac tgcggtcatc attccctggg aacggaagat ttctgataca
     4561 tagatacagt tcataacggt tgccagcata ccgatctggt ctgctttggt acggtcgata
     4621 ttttcggaac tgcgtcctct ccagaagttt cctcctccga ttacgatgcc gacttcaatc
     4681 ccctggtcta ccagagtttt tacctgtttt gccacttttg tgacggtcgg ctcgtcaaaa
     4741 ccggtgtgtt tctctcctgc aagagcttct ccgctcaatt tcagtaatac tcgtcgtttc
     4801 atcttgcatc ctcgttcctt tatgatattt tttcacaaat gtcttgcatt tatgaaatga
     4861 ttataccatc tgttcccaaa aacgacaagg gcaaaattcg tttctgttta cctgcggtac
     4921 tcggtcggcg taaggccggt gtagccttta aatctctttg aaaaataccc cggtgaggaa
     4981 aattgatagt tcagtgcaat tgacataatc tcttcgtttg tgttccgcag atcggttacc
     5041 gcgctgtctg cttctgcgaa ccgacgacct ggatcatctg ctgtgtggta atctgtattt
     5101 ttacattcac cattcgaaaa gaattccgcg gtacggcggc ataaaaacca taaaaaaatc
     5161 tctgtcggaa gttcagactc ctgacagaga ttttttatag attcatttta gatctgtgct
     5221 actacaaaga tatcatagat tgctttgatt gccgtctcaa aatcgcggtt ctccacgccg
     5281 atgatgatgt taagctcact ggaaccctgg tcgatcatct taacgttgac gtgtgcgtgt
     5341 gccagtgcgg agaagattct tccggcagtg cctctcgttc ttctcatgcc gcggcctaca
     5401 acagcgatca gcgccagatc ggactcaagg tcgatggaat ccggctgtac cgcacggtgc
     5461 agtcctgcga tgacctgctg ctctttttct gcgaactcgt tctggtgaac gaatacagtc
     5521 atggtatcga tgccggacgg catatgttcg aaggaaagat tgttgtcctc aaaaacaccg
     5581 agcacttttc tgccaaagcc gatctcggta ttcatcatgc tcttctcaac cgtgatggat
     5641 gcaaagcctt tctttccggc gataccggtg atggtgaatt tcggtttgct gcaggtgttc
     5701 tccacaatca tggttcccgg atcctgcggt gcgttggtgt tcagcacgtg gatcgggatt
     5761 ccctcctgac ggagtgggaa gatggagtcc tcatgaagga cggtagctcc catgtaggaa
     5821 agctcacgca gctcacggta ggtaatggtg gaaatgactt ccggatcctt gacgatacgc
     5881 ggatcggtta ccaggaaacc ggaaacatcg gtccagttct cgtagatgtc agcgtgtacg
     5941 gcctttgcca cgatggaacc agtgatatcg gatccgccgc gggagaaggt ttttacgctt
     6001 ccgtccggtt ttgcaccgta gaatccagga acgactgcgt tatccagatc ttttaaacgc
     6061 tcggagagca cttcgtcagt cttgtctgca aggaagtttc cgttctcgtc gaagcagatc
     6121 acttctgcag aatcaatata cgtatatccc agataatttg ccatcacgat accgttcagg
     6181 tactctccgc gggatgcggc gtagtcctct ccggctgctg cggcaaagtt ttctgcgatt
     6241 ttttcaaatt ccggggaaag atccaggctg aggttcagac cgtcgatgat ctcctgatat
     6301 ctcgctttga tgttctgcag tttcttcttg aaatctcttc cagcaactgc agtgcgatag
     6361 cagtcataca gcatatcggt tactttcgta tcagcagaat ttcgttttcc aggagcggaa
     6421 ggtaccacgt atcttcttcc ctcatctgcg cggatgatgt ctccgacctt cttaaactgc
     6481 tctgcgctcg cgagggagct gccgccgaat tttactactt tcttcataat aatctctcct
     6541 tgcgttcttt tcggatatct ttcgaaatct cgtttttcag cccgtcactt taacgttaga
     6601 aagctgttac gtactagatt agaacaaaat cccgtatgcg tcaagaactt tttgggctga
     6661 tttattcatt ccacagataa atttccggtt ttttctccat ttcataggag aatgtctctc
     6721 caagcccgta aaccgcctgg cttaatacgt actcgccgtt gtttgcatag atttcgatca
     6781 tattcggatc cacgaaaatc tcaagaagat ccccctcttt taactgcggt gtctcggcat
     6841 gaagccatcc ttttgcaccg gatggataga catccgtgcg gtctgcacag acttttccgc
     6901 tctgccgcca gatcttatag ccgccgattt ccaggctctc accctctttt aattcggcgc
     6961 ggatcatggc acactctccg catgccgaaa cgagggaatc cgtctttttg cagaatccgt
     7021 cgcggatggc aggcagaaca cgaaagtaaa tatgatcgtt ctgtacatcc accacacgtg
     7081 gcatgcagaa catgccttgc catataccat ctaccggttc cggcatccga agccatgcga
     7141 tggaaatccg gtttccgtcc gcatccgtca tggtctgcgg tgcatagagg tcaccgccgc
     7201 agtccagaag ctgccagttc tccggaaatt tcattgtgca ggttttttca tcaaactcag
     7261 gcagcataca gatcgcccga tccgcatagc cgtcggtgtc ctcgatcagt tccatcgggg
     7321 agaatacaaa gacgctgcgt ccgtttacgt taaagagatc cgggcactcc cacatccagc
     7381 cgagcttctg tttcgtcgtc gcctgtccgg ccagatgcca gtgggaaaga tcctcactct
     7441 tatagaataa tgcctcgccc ttgtgatcct tcgtcgtgct tccaagcacc atataccatg
     7501 cgtcgcttcc cttccagact ttcgggtcac gcgtgtgcgt attgtcgccg atttccggat
     7561 cggtgagtgc cgggataaca acctccttat ccccgaagac atcaaaatgc atgccgtcct
     7621 tcgaagtaag gtgaagctgc gccgaggtaa aatcctgatc aaggcactga tgaatatcct
     7681 tcggatttac ctgattatag tgaacacccg tgtaaaagag atgcatcacg ccgttctctt
     7741 ctactgcgct tccggaaaag cagccgttct ggtcctcgga aagactcggg aacagggcaa
     7801 cgccgacatg ctcccagtga atcagatccg gactcaccgc atggccccag tgcatggtgc
     7861 cccagcgcgg cgcgtacgga aaatgctgat aaaaaagatg atactgtccc ttataataaa
     7921 tcatgccgtt cgggtcattc acccagttgc ccggcgcttt tacgtgtaat ctgtttttca
     7981 tgtgtttgat ttcctttctg tctctttctt gttgtctctg ataagaaaag ccggaaaaag
     8041 aaagcatttt taattccgcc ctcctttgcc cggcttgttc ttgatttcta tgatttactt
     8101 atttgactgc tccggctacg acaccgttga tgatctgttt ctgcagtgcc acgtacagaa
     8161 tcaggatcgg gatgacgcag agcagaagac ctgccatgat ggttccgtaa tctgcggagt
     8221 aagttccgta gaagctgtag gtggaaagcg gcagagtcag cagctttttg tcggtcagca
     8281 ccagggacgg cagcaggaag tcattccaga atgccatcgc attcatgatt accatggtgg
     8341 aaatgatcgg ctttaacagc gggaacacga tgcggaagaa ggtctgggtg tgggtacagc
     8401 cgtcgatgta agcggcctcc tccagcgcca gcgggatact tccgcggatg aatccgtgga
     8461 acatgaacac ggacatcgcc atggaaaatc cggtgtgcat gaagatcagg gtcagtctgt
     8521 ggttcagaat gtttaaggtt ccgccgtata cactgacaag cgggatcatg attgcctgga
     8581 atggaatgat catggatgca accatcagtg caaagatgat attggaaaat acgttgacat
     8641 tacgcacgat gtagtaagcc agcatggctg cgagtaccgt aaccagtacg gttgctgtta
     8701 cggttacaac cagagagttc ttgaaggcat tcaggaagtt catctgattg aatgctttta
     8761 caaagttgtc aaacgaaaga cctttgatgg taaacagcca ggagaacggg ctcttgatga
     8821 tatcacgttt ctgttttaag gagttgatga taaccatgac aaacgggaac atgaacgcac
     8881 agaaaacgat gatcataagg agcatagcca gcgcgtcagt tacttttttc tttgttccgc
     8941 caattttctt ctgattcatt atgcttcaac ctccctcttc ttggtggtgt atacctgaat
     9001 accgctgatg caggctacaa tgataaacag gataaaggct tctgcctggc cgacaccgaa
     9061 ctgtctcgat gtgaatgctt tctcatatac gtgcatggct gccatctcgg tggttccgta
     9121 cggagcaccg ccggttaagg acaggttgac atcgtatacc atgaacgcac gggacagggt
     9181 caggaagagg cagatagtaa cggaggacat catcagcgga aggataatgt ttttcatttt
     9241 tacccagccg gatgcaccgt cgatgcttgc cgcctcgagc acgtcctcgg aaagtcccat
     9301 aaagccggca acgtagatca ggatcatgta gcctgccagc tgccatactg aaacgagcac
     9361 cagtgctgca aatgctttgt tcggggtaga aagccaggaa atttcaaaca ggctccatcc
     9421 tgtggtctcg ccgatgctga cgaaaacacg ggagaatacg aactgccaga tgtatccaag
     9481 tacgataccg ccgatcaggt tcggggtgaa aaatccggcg cggaagaagt tctgaccttt
     9541 gatgccgcgg gtcaggatgt aagccagtaa aaaggcgatc acattgacga gaacaacaac
     9601 cgcaatgaca tatttaaagg ttaacagcag ggatttccag aactgcgcgt cgcgcataac
     9661 ggctgcgaag ttggaaagtc ctacaaagtt tttcacgctg gaaacaccgt tccagtcggt
     9721 taaggtcagg taaataccgt agataaacgg taccatgacg actgccagga agcagaacag
     9781 gcccggcagc gcaaatacac agaaatcttt tcccttgttg cttgatttca ttttcgttcc
     9841 tccttcatat cctccggctt actgctcatc ccagtatgcc tggatggaat ctatcagttc
     9901 ctcgcggtca cttcttccgg ccagatattt ctgcattgcc gcaccaagga cagaccagtg
     9961 atcgttcggc acgatcgcag atgcgttgaa tgctccgccg ttctgtactt tttcgtaaat
    10021 gtcgcggctt aacggatcgc ttggctcgtt cgggttgttt ttaaacggag ggatcaggct
    10081 gcaggtcttt acaaccatct gctgtccgat ctcactgtaa accagccagt tcagaaactc
    10141 ttttgctgct gctttctgct cgtcggtagc cacgatatca tccagcatga tctgtttgga
    10201 cggggatgcc tggattttgc tgttgacgaa gtcgtccgga tcattgttca ggaagtatgg
    10261 aaggaaacca tactcatcgg tcgtctcggc tcctgcctcc tcaaggttcg gccatgccca
    10321 gtttccgttg aaccagaaag cggtttttcc gtctgccagg tcaattgcca tctcatcgta
    10381 atctgcgccg agtggatcgc cctttgctac gttgtactct tttaacaggt caaaggtatt
    10441 caggaactcg ttcatgcggg tgtagtccgg cagatataag gttccgtcct tcagctcgtt
    10501 gatgacttct gctgcaccgt cggaggttcc gttgtatgtc tcgtagatgt actgcagctg
    10561 gtgtgctcca agggaccagt cttcttttgc catggagatc ggattcttca ttccgttgtc
    10621 ccgcaggctc tggagaagtg ctgcgaaatc gtcctgggtt ttgatatcgg atggatcgaa
    10681 ctcggtacct aaggtatctt caatcacgct cttgttgtag ataataccgc ggccctcgat
    10741 acagagcggg aagctgtaaa cctttccgtt gattttggtc aggtactctt ctgcctcgcc
    10801 ggtccatgct tcattgctta agtcggctgc tttttcttct gccagtgcaa tgacatcggt
    10861 cgtatccaga atggaaagtg tcggcgggtt tccggagttg tagaggctga ctactttggt
    10921 ataaggagaa tcgccgtccg ttaccggcat gatctcaata tgtacgcctg ctttctcctc
    10981 gaacactttt gcaatctttt ccagtgctgt ctggatttct gcttttgtgt tcatgatgac
    11041 gatactcgtt ccggaaagcg acgtatcgta ctggaaggta tcggtggtca tgtcgatcgg
    11101 cacttcttct tttactgcgt tcgggtcatc cggatcgctt gcgaagccga aggtgcatcc
    11161 ggtcagtgca gaagtaagaa gcacgccgga cattccaaga gctgccgccc tggcactaaa
    11221 tttctttctc atcacaaatc ctcctgttat ctctggacag cgggtcctgt ccttcgtttt
    11281 gtgtaaccgg tcaagtaacc ggttactttc attgtcttta tcatagcatt gacagtgggt
    11341 ttcgtcaata ttttctttac ttttcgtgat tttccataat tttgtttttg gcttttgtgg
    11401 aacttttaca aaaccggtta cttaaccgat tcccgcttgt tccggcgggc gagaaatgtt
    11461 atactatctt catatattat ttgttttttt ctgaaagctt acatgattca cgcaacactt
    11521 ttgaagggaa ggacacttga acttatggca cgaaaaaaaa atgtaacctt taatgatatt
    11581 gcacagtaca cgcatttttc caaaacaacc atttcccggt atttcaacaa cccggattcc
    11641 ctgacaccgg aaaatcagca gatcatttcc gatgcactgg ataaactgaa ctataaagaa
    11701 aacaaagtgg caaaaattct cgcgaacgga cagaccgaat ttgtcggcgt cctgatcccg
    11761 agtctctcca tgaactacta ttccgagatg ctgaatcaga ttcttgcttc ttatgagaaa
    11821 tatggctata aattcctcgt cttcgccgga aacggccacg acgagacgga gcaccgctac
    11881 attcaggagc tgatgtcgta caaaatcgag ggcctcatcg tcctctccca tacactctcc
    11941 tcccgcgaac tctccgatct gcagatcccg gtcgttgcaa ttgagcgcga agaccagttc
    12001 gtcagcagtg tcaatacaga caactacctc ggcgcgtacg aagcgacttc gcttctgatt
    12061 cataaccact gtgatgtcct gattcatctg aactcaccga ccaaagaaaa tgttcctgcc
    12121 taccgccgtc tcgccggttt ccaggatgcc tgtgtaaagg ccggtgtacc gcacaaggtt
    12181 atcattcggg attttggact ctcctatcag gaagccgcag caccgctgca ggaaattttt
    12241 gacaacatcg aacaaaaata tgccggtcag aaaaagggca tcttcctctc caacgatgat
    12301 catgccaata ttcttttaaa tcttctgatc cgcaaatacc ataccctgcc ggacgattac
    12361 gaactcatcg gcttcgacgg tgcgccgatt tccgagcagg ctatctatcc gatcagcact
    12421 gtcggccagc agattgacca gattgcagac gaggctgttt cccttctggt ctcccagatg
    12481 caggaacgga aaaaacgcca cccggtgccg cttgccgagc cggttcacaa gaccgttccg
    12541 ccgcttctga tccggcgtca gacgaccact tgattttaaa agcagatttc tatcataaaa
    12601 atgtttttat caaaaaagtg tcatccacct gcgggctgtg tgccctgcag tttgtggatg
    12661 acacttttct tatcttctta ctctttttta gttctgtccg taataagcat ttgcaccatg
    12721 cttacggtaa tagtgtttat cctgcagctc ctgcggtgcc ggctggattc tcgggttgat
    12781 ggtctcggca cggtatgcca ttttggcaac ctcttcgagg acaacagcat tgtgaacagc
    12841 ctcatgcgca tctttgcccc aggtgaacgg tccgtggttc ttgcaaagaa cggccggaac
    12901 ggcttttaca tcttttccca tccgtttgaa ctcattgacg atcagatggc cggtgttttc
    12961 ctcgtatgcc tcgtcaatct cttcttttgt caggcagcgc aggcacggta cttcaccgta
    13021 gatgtagtct gcatgggttg ttccatagca cggaatgctt cttcccgcct gtgcccagct
    13081 tgtcgcatag gaggaatggg tgtgaacaat gccgccgatc tccggaaatg ctttgtacag
    13141 ctccagatgg gtcggggtgt cagaagacgg acggtagcgt ccttctactt tgtttccgtt
    13201 cagatccatg acaaccatat cgtccggtgt cagcaggtcg taatccacgc cgcttggttt
    13261 gatgacgaac agaccgcttt cccggtcaat ctcactgaca tttccccagg taaaggtaac
    13321 cagtccgtat tttggaagaa gcatattggc ttcgtatact ctttttttca gttcttctaa
    13381 cattgtgttg tcctttctgt ctttcccaaa cgatggttca ttttaagctc tctactgctg
    13441 cacgctcaat cggcaggcct tttttgtagc gctcggtgaa tacttcaaat ccagcgacgt
    13501 ctgccgggtc cggatccatc ttggttccgg catttcctgc gaagactttt gcatcgaggt
    13561 aatcctcgag tgtttcattc tcttctttgt tgatcatata agaagccagc agtgcgatgc
    13621 cccaggctcc gccctctcct gctgtctcca tgacggaaac cggtgcgtcg atggcacctg
    13681 cgaggatctt ctggccgaca ccttttgtct tgaagagacc gccgtggccg agaatctggt
    13741 cgacctgcac atgctcctct ttcatcagga tatcaaggcc gactttcagg gcgccaaggg
    13801 aagtaaacag gttgactctc ataaaatttg ccagattgaa tttcgcatcc ggagtacgtg
    13861 cgaagacagg acggccctcg ttgaagccgg ttacgtgctc gccggagaag tagttgtagg
    13921 caaggagtcc gccgcagtct gcgtcgccct ccagggcttt gttgtacagc ttgccgaaca
    13981 gatcattcgt gctgatttcc atgccgaagg tctgcgcaaa ttcacggaag agattgaccc
    14041 atgcattcag atcagaagta cagttctggc agtgcaccat ggcaaccgga tgaccggacg
    14101 gggttgttac catatcgatc tccgggtata ctttggagag gttcttctcc agaacgatca
    14161 tagcaaaaac ggaagttccg gcagacacgt ttccggttct gacgcggacg ctgtttgttg
    14221 ctgccatacc tgttccggca tctccctccg gcgggcagag cggaatgcct gcttccagat
    14281 ttccggacat gtcgagaagt gcggcacctt cttttgtcag aacaccggca ttgtcacctg
    14341 cggtcagaac gtgcggcaga acatcgagtg ctttccatga atatgctttg tctgcgactg
    14401 cctcgtcaaa tttggcgatc atgtcggcaa agtaagtgtt ggtttcagaa tcgattggaa
    14461 agattccaga tgcctcgccg ataccaacga cacgctcgcc ggtcattttc cactgaatgt
    14521 atccggcaag ggttgtcaca tagtcgatat ctgccacatg cggctcaccg tttaagattg
    14581 cctgatacag atgggcgata gtccatctct gcgggatgtg gaatccgaaa agatcggtca
    14641 gcttctcgct tgcttcttct gtgatattgt ttctccaggt gcggaacggc acgaggaggt
    14701 ttcctgcttt gtcaaatgcc atatagccgt gcatcatggc actgaagccg atggctccga
    14761 cgcgggtcag ggtgatgtcg tatttttctt tgacatcagc tgccattttg gtatatgcat
    14821 cctgcaggcc cgtccagatg tcctcgaggg tgtaggtcca cacgccgttg tcgtagcggt
    14881 tttcccactc gtgatctcca gatgcaatcg gggcgttgtc tgcgccgatc aggacggctt
    14941 tgattctggt tgagccgaac tcaataccaa gtgctgtttt ctgttcgagg attgctgttt
    15001 ttgcatccat tcccattgtt ttttcctccc ttgggttctt ttctattttt tcgtctctgt
    15061 tgacagattt tttcctgcag tggattctct tacgatgagt tccggggaaa tcaggcgttt
    15121 gacgctgctt tcttcttccg ggacttttct gattttttcg agaatgagct ctgctgccat
    15181 ctcaccgagt ttttcctgcg gatgggcaat ggtcgtgatc ccgatcggac tgctctggcc
    15241 gatcagcgag ttgtcgtatc cggtgacggc gatgtcttcc ggcacggaga ttcccttttc
    15301 ttccagcatg gagatgacgg cgagcgcgat ctggtcgttg tagcagacgg cggcatcgac
    15361 ggactttttg ctgtcgagca gcatggaaag catcagggac ggtttggttt tccggtcttc
    15421 ggtgtgaaac cagatgacgt tttcgggatc atacaggatt ccgctttcct gcagcgcctg
    15481 cacgtagcct ttatggcgct gggcgccctg gctgtcgtcg gccttgaaga tgccgatgat
    15541 gtttcgtcgg ccggtctcta aaaggtgccg ggtcagcaga tagccgcctt tgcagtcatc
    15601 catcagaatg cagggcttgt ctgccatctg ggggtagcag ccctggatga agacatacgg
    15661 gatctggtac ttgtccagca tttcgtacaa gttgatgtgg cggcagagga tctggctttt
    15721 gctcggctcg atgatgaggc cgtcgatggg cttgtcgagg agttctttta agactttttc
    15781 ttctttggag cggctgttgc aggtgttctt taagataatg ctgtagccgt tttcgctcag
    15841 cactcgatcc attccctgga tgaggcgggg gaagatgtag tcggacaggt aggtggtggt
    15901 gacggcgatg ttttttgaat tatgacgctg gattacgcgc tcggagcaga aggttccttt
    15961 gccgtgccgg gcggtgatgt agccttcatt ttcgagaaga gcgagggcct ttcggacagt
    16021 gtggcgtgag agggcgtatt cccgggtcag ttcgttttcg gagggaagct tctggccggg
    16081 ttttatggtt ccggagagaa tctgctcttt tagggcttcc atcagggtgt agtatttggc
    16141 tttgccggtg gttggttcca tgtgattggt gattcccctt tctggaattt ataatacaac
    16201 ttgtacggtt gttgtgacaa gttgtatttt tgttgtgggg aagggacgct caggagtatc
    16261 gggtgtccgg ggcgtggctg tttcggaggc taggaactct aagggtgtga aaatttgcgt
    16321 tattccccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa taacgcaaaa
    16381 ttccacgccc taagggttcc tacgcctcct gcaaatgcca cgccccggac acccgatgct
    16441 cctgagctga ctttggcccc gtaaatggag ggaagcggtc ctgcggtggc ttcccttggt
    16501 tcaccaatag agtaaatggg cgggcgggtg ggtccaatac cttaatgagt ggacgggaac
    16561 acttttttac ggatggcttc ttcataggcg gtgcattctg cagaccggtg catccgcggg
    16621 acgggatctc ctttgaggaa gtggtagcgg atgtaataga gggtatcgtc ttcgccgcgt
    16681 tccgagagca tgcgcagcag aaattcgagc tgcgagagtg tctcgggatg gacgaaccag
    16741 aggtggccga ttcctttttt gagatactgg tacggcgcct gatcggtata agcgtctccg
    16801 aggtagacgc gggacgcgct gatgcggtcc ataatcatct cggcgacgta gcggcgcggc
    16861 attgggacgc ctttgatgat ggtgtcacaa ttgataccgt aatcaatcca gtattcaaaa
    16921 tgatgtttgt tgcgcccttt atggtgcatc caggattttg acatgccggt gtcttcgcgc
    16981 tcggcgttat tgggactccg gtcgccctga tagtatttca tgccgaccag aaattccgtc
    17041 ggcgaatatt tcgagaggtc gtgggtcagt ccctgccagt acagtcctat tttgaagcag
    17101 taccgcatga cccacatttt gtgtgtggta atggttttaa agtgctgcca tgctttcatc
    17161 attctgtctc ttctcctgct ttcctgttaa aattatgata ctgcgcggcg gcacgatgac
    17221 cgtttttgcg ctgacttttt tctgattttc ttctgtcagg aatacttccg gtgtgttggt
    17281 gtcggccgtc agataccaga ccttctgttc cgggagggac ggcagtgcca gctcatgcgg
    17341 attccagtgc atgttgtagg caatataaag atcgtcgtcc ttgttgccgt ctttatcctg
    17401 gaaatagctt ccgcagtaca tgacgccgat aaaacggcat gtgttttcca tctgggagaa
    17461 ccaggctctc tggctgtgga aggacatatc cgggcagccg taggatttgt agtcgatgag
    17521 gcgcggctcg cgttcgccgt gcagcgccgg atgcgctttc cggaaggcga ttgctgcttt
    17581 tacaaaggcg gcaaactccg gattccgggc ggctgctttc cagtcgatcc agccggtttt
    17641 gttgtcctgg caccaggcgt tgttgttgcc cttctggctg ttgccgaatt catcgccgcc
    17701 gtaaatcagc ggtgttccct ggcttgtcat cagcatgaga acggcatttt tcagctggcg
    17761 gagacgcagc gcgcgcacag ccggtttccg ggtcacgcct tctgcgccgc agttccaggt
    17821 caggttttcg ttggaaccat ccatattgtc ttcgccgttg tccagattgt gtttctgttc
    17881 gtaggaaacg ctgtcataca gggtaaaacc gtcgtgatcc gcaagataat tgatcacacc
    17941 gtgtgtctcc gggcttcttc tcagataata ggtgaagtcg gaaaggctgc cctcatcgcc
    18001 ttttaagaag cggcggatat ggtactggta gccctgattg tgttccccta aattccggaa
    18061 ccacgggcgt tttccctgat agatccgtgc gccgtcaaaa ccgaggaaaa tcagtttcgt
    18121 cttccgaagg agtgcatctt tggacgcttc ttctgccagc gatgcatcgc cgaccagatg
    18181 gaaaccgtcg atctgatagc ggagctgcca gtggtgcaga acctgcagga caaaaccaga
    18241 aggcgttccc ggggcaaaac agaattccat caggcattcg ataccattct catgcagggc
    18301 atcgaccatc tctgcgaact ctgcgacggc atctgaggat gcagcatacg aagctttcgg
    18361 agcaaaatac agggcctttg tgccgtatcc ccaataattc acgcgcttct cttccgccgg
    18421 cgccggatat acataggccg gctccgtaag ggaggtccac tcaaaaaact cgtaggacgg
    18481 catcagctct actgcattga taccgagttc ttttaagtat ggaatttttt cgatcacgcc
    18541 gcggaacgtt cccttgttct ttacacggga gcttccgtct ttggtgaagc cgcgcacatg
    18601 gagcttatac aaaatcgtct cctcatacgg ccggtgcagt gaggtggtgc gcattaccgg
    18661 gtactccggg agaaggcagc agcaggcgcc ctcttctttt tccatcggtg caccgaaggt
    18721 ttctcttcct tctactgctc ccgcatacgg atccaagcag aaagcttcgt ccatccggta
    18781 ggcatacgaa aactttccta ccgtcaggcc tgtgatttcc acggaactga tcacgccagt
    18841 gcgctcctct tccggaagcg gcacggtaag aaacggtttc tggtgacctt tccggtacag
    18901 cagaagctct gctttttttc catctgatac ttctacggca aaattgtatc cgtctttcgt
    18961 cgccgatgcg cccattttct ccggatttcc cggtaaaatt cgatattttc ctctgttttt
    19021 tgtctgtatc ataattcccg cccttatttt ttataatttg ttgcaattgt catactatta
    19081 atgtaaatca tttgaataaa attgtcaatt gaccaatgtt accaattgtt atacctctaa
    19141 atccagttcc accgggcaat ggtcggaacc gaagatttcg gtatggattt tggcatctgc
    19201 aactgcatct tttaaggact ctgataccat aaagtaatca agccgccatc ctgcattccg
    19261 ctggcgtgcc tggaagcggt atgaccacca ggaatacgtc acggtgtccg gatacagcat
    19321 acggaaggaa tcaacaaagc ctttttcttt gatcacagca aatttttcgc gctcctcttt
    19381 cgtaaagccg gcgtttttct catttgcctt tgcatttttg atatccatct cggtcgctgc
    19441 gacgttcaga tctccgcaga aaatcaccgg tttttcctgc tccagtcctt ttaagtaaga
    19501 aagaaacgca tcctcccact ccatccggta ggcaaggcgt tttaattcat cctgcgaatt
    19561 cggtgtgtaa acggttacaa caaagtattc cggaaactct gccgtaatca cacggccttc
    19621 cgtatcatgt tcttcgattc cgataccata gcgaacagaa atcggctcct ctttggtaaa
    19681 cagggccgta ccggaatatc ctttcttttc cgcataattc caatactgat gatagccggg
    19741 cagctcaagg tcgatctgtc cttcctgcag ctttgtctcc tgcaggcaga aaatatcggc
    19801 atctgccgcc tcaaagtatt cctgaaagcc tttcttcatg caggctctca gtccatttac
    19861 gttccaggat atcatttttt tcatacgcaa ttcatctcct tttcctgaca gtctgttctc
    19921 tgcggtttct gttgcaaaac gccgggaacc gacttccgtt ttcttttatt cctctttcag
    19981 aacatacagc acttccagcg acgcatccga cggtccgtga aattcgcagc cgtctgcttt
    20041 atactggcgc atcagttcca acatttctct gctgtaccgc tcgcccgggc agatgagcgg
    20101 gattcccggc ggatatgggg ctgccatctc ggctgcaatt cgtccttccg cttctgcaaa
    20161 cggaacggct tccttctccg caaaatatgc ctcgcgcggt gtctttacca tctccggcag
    20221 gctgcggaac agccactgct tttctgtaaa cttcgtccgc cccgtcgttt tcccgctctc
    20281 tgctgccgaa attcctgcca gcgcatcgat cagccgtgtg atatcttcct cgctgtttgc
    20341 ccaggtaata acgcagacca cgttctcctc gtcggcaagc tctgtactga cgcggtattc
    20401 ctcgtacaga cgcacctgca gatcgtagcc ggaaatacca agttctcttg ccgagaatac
    20461 aagacgcgtc ggatcgatct tccatacgcc gcacgttcct tccatctcgg tcccaagaac
    20521 ttcgattcct ttgatctttg caagctcctg ccgcgccctc tggccgagtt ccagcgcgcg
    20581 ttccattcgc tcttttccgt gcagcgccat cccgcgccgc gccgcatcga gagatgccat
    20641 cagaaggtag gacggtgatg tactctgtac catctgcaga ttggcggcca ctcttgcctc
    20701 atcgagacgg ccagaaccga gtgataagaa ggagctctgt gtaaaggaac ccgccgtttt
    20761 gtggatactc tgcgcgcagg catccgcacc actttccagt gcgccgagag gaagctgttc
    20821 ggaaaaatag aggtgcgatc catgtgcttc atcgacaaga agcgctgcgt tgtggcagtg
    20881 acagatttct gcgatcgcct gcagatcact gcaaagaccg tagtacgtcg ggctgaccag
    20941 cagcacggct ttcgcatccg gcgtttcggc aaacgctttt tcgatggttt ccggcgtcac
    21001 gccgccccac atctgccatt ttgtgctgta ctccggcatc acataaaccg gtgtggctcc
    21061 gcataaaatc agtcccatca gcaccgattt gtgcacgttc cgcgggacaa tgattttttc
    21121 gccctctttc acagaggaaa gcaccatcgc ctcgttgccg catgtcgtac cgttgacgag
    21181 aaagaacgtt ttttttgcct tccacgtttc tgccgccaga cgctgcgcct ccgcgatcac
    21241 gccctccgca tggtgaagat cgtcgaggcc ttctgcctcc gagaggtcat acgctgcaag
    21301 cgatgctccg tttagcgcct catccagccc ccgctcaaac cggtgtcccg ggatgcggaa
    21361 ataagccggc gcttcctccc tgaactgttg taacgcctcc acaagaggca ttcgattctg
    21421 atccattctg ttttgtttcc tttccaatgg tttctccatc tttttctgaa acgaaagaat
    21481 cctccccgca ccgccgtgcg ggaaggaccc cttattcgca tttttctgtt tattatggtt
    21541 tattctaccg gagtagtcca gccatatttg tcttccagct tgccccactg gataccggtc
    21601 agggtatcgt acagtttctg ggtcagctca ccgatcttga aatcgttgac gataacggaa
    21661 tcgtctttgt atacgaactc gccgactgga gatacaacag ctgctgtacc ggtaccgaat
    21721 gcctcttcca gtgtgccgtc atggcctgct ttcatcaggt cgtcgatgga caggtgggtc
    21781 tcgtttactt tgtagcccca gtcacgcagg atgtggatca gggaatcacg ggttacgccc
    21841 ggaagtacgg ttccttccag cggtgcggtg tagatttcgc cgtcgatctt gaacatgatg
    21901 ttcatggttc caacttcctc tacatattta cgatgtacgc cgtccagcca cagaacctgg
    21961 gtgcatccca tcttctctgc ttttacctgg cctgcgatgg agcttgcata gttaccgccg
    22021 cattttgtga agcctgtgcc gcctttgact gcacggacat attcatcctc aaccaggatc
    22081 ttaaccgggt tgatgccctc tgcatagtat gctccaaccg gagtggtgat gataacgaat
    22141 ttgtaggatt ttgccatatg tacgccaagg cttgcctcag tagcgaacat gaacggacgg
    22201 atgtaaagag atgtacctgg ctcagacggt acccagtctt cctgtgcttt aaccagtgct
    22261 ttgacagctt ctacaaacat ctcctccggt acacccggca tgcagagtct ttcgttggat
    22321 ttgatcatac ggcgtgcgtt catttccgga cggaacagct ggatttttcc ttctgctgtt
    22381 ctgtatgctt tcatac
//
LOCUS       DAOMUF010000013        22101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_376_length_22101_cov_3.415132, whole genome shotgun sequence.
ACCESSION   DAOMUF010000013 DAOMUF010000000
VERSION     DAOMUF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 22101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22101
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_376_length_22101_cov_3.415132"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLN77_01895"
     CDS             <1..225
                     /locus_tag="PLN77_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43068.1"
                     /translation="SDFQPQGRYSMPMLIPLMYFVTKGVQAFLKREKKLQKYEKLFCI
                     LVCVAAAALAAFTWARIIYPMYLQNYYGLR"
     gene            256..2460
                     /locus_tag="PLN77_01900"
     CDS             256..2460
                     /locus_tag="PLN77_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM43069.1"
                     /translation="MEKISVLTAKLKKAVAEEGIGGLAGRTKGYLARAKKEKEFQREK
                     SRVYRDILFISGCNEQLPHPHRYRVVHQMEQLEAGGYTCDTVYFQELKPWMVRCYGAF
                     VIFRCPMTDTLREFATMAKQMNKPLWYDVDDLVIDTKYTDQIPFLDRMQPEERQAYDQ
                     NVRNMGELLSLCDAAVTTTAALAEELKQYVPEVLINRNCASDEMLLLSEAVLKEKQKK
                     NEADGTAKKVRLGYFSGSATHLDDIEMIVPVLKQLLGKNPNLELLIVGILELPVELKL
                     FASQIQMEGFVDYQKLPERIASVDINLAPLTDTIFNRAKSENKWVEAALVQTVTAASN
                     LGAFAEMVQDGEDGVLCRDEAEWLEKLQWLIDDEPARKAIAGRAYGRCSRECVTIFHA
                     TGICEWVERHWNLRCAFVLPAMEISGGIRVALLHAEMLVKAGAQVSLFTLEGEAEWYH
                     EGDFHFPVLSAEREKLQGTLDLAVATMWNTAEFVEQSSKIRKKKYLVQNFEVGFYPPG
                     SPYRIATSATYRMRSPMEYVTISKWCQNWLREEYHTEAVYLPNGIDPSFYPKRGRDLQ
                     GKIRILIEGDCSAEHKNVDESFRIVEQLDLEKFEIWYMSYNGNPKSWYRVDRFLHRVP
                     YEKTPEVYAACDILLKTSLLESFSYPPLEMMASGGYVVAVPNGGNLEYLKDGENCILY
                     PQGNLAEAKAAIERILTDAELRKKLDTGAEETVKERNWKRIEPQILEQYLGK"
     gene            complement(2655..3962)
                     /locus_tag="PLN77_01905"
     CDS             complement(2655..3962)
                     /locus_tag="PLN77_01905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRM43070.1"
                     /translation="MIRKNSAARTFLCLLLAFVFAASCLPQTIFATTDKKTVTTWPEG
                     PENNSGAVCLLDADTGAVLYDKNMDEQRYPASITKILTALLIIENKQMTDTVTFGEYA
                     VSESIPGNARINVQLGETITVEDALHAILLASANEVCTQLAIDIAGSEEGFAAMMNER
                     AAALGCTNTHFVNANGLPDPNHYTSAHDMALIMQECIKNETFCRIESDLTYTIQPTNM
                     TSTPRDLQNHHALLFQDGQWGYKGAFAGKTGYTDEAHNTLVTAAKRGNMTLVCVVLTC
                     DNLDYINDTRTVLDFGYDNFTHYTLKNAKKEILSGVVTLPKNAKIETATYTDPDGASV
                     EEPYTRQYFFDDHFIGTAEVSAPGTVSDSSSVSSDSSEETDSTDIPTPEETKKISFLS
                     TLLYILLGLNGVAFLILIIVVINNARKRRKRRNRRRNNRNKRH"
     gene            4214..6049
                     /locus_tag="PLN77_01910"
     CDS             4214..6049
                     /locus_tag="PLN77_01910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM43071.1"
                     /translation="MKWLRFIRKLKPYNIQKGLRYLKHYGPKEFLIRLSERMEPEEVP
                     YGPWREQYCAKEEDLARQKNHKFSYCPKISVVVPAYRTPEIFLRQMIESLEKQSYENW
                     ELVIGNASPEDETMARILKEYTGKDPRVKNVPIPENIGIAENTNAALAAAAGNFVGFM
                     DHDDLLAPDALFEIAIRLEKDPSIDAFYTDEDKVRTDLSEYFQPHFKPDFNLDLLRSN
                     NYICHFFVVRREIAEKTGGLRPEYNGAQDYDYIFRCTEMAGKIVHIPRVLYHWRVHSA
                     STADNPASKLYAYEAGKKAIEGNLARCGEEGTVTLRSDYGFYDVDYKLRGTPLVSILI
                     PNKDQADTLRTCLESIKKKCMYPSYEILIIENNSTEEATFSYYKELEAQGIRVLKYPK
                     QGFNYSAINNFGVKEAKGEYLLFLNNDMEIITPDFIEKMVSNLQRPQIGAVGAKLYYP
                     DNTVQHAGIIIGIGGIAGHAFLGLARGRSGYLHKASLQMNVSAVTAACMMMRKEVFEE
                     VGGFEEELSVAFNDVDLCLRIGKAGYKIVYNPHVELYHYESKSRGAEDDEKKVRRFQS
                     EIEFMRSRWIGLLKAGDPCYNPNLTLASWNYGLRADGRGVKPWIK"
     gene            6037..7011
                     /locus_tag="PLN77_01915"
     CDS             6037..7011
                     /locus_tag="PLN77_01915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM43072.1"
                     /translation="MDKVAVVIPNYNGYRYLETCFAALAKQTFTEFTTYFVDNGSQDE
                     SCAFLREHYPETKIIQLDDNYGFSRAVNEGIRQSKEPYVILLNNDTEVFPDFVERLYE
                     GICKRKNAFACSAKLISYQERDKIDDAGNYYNMLGWAFARGKGKPVSEFETSDRIFAA
                     CAGAAIYRREFLEKTGLFDEEHFAYLEDTDIGYRARLLGYENWYEPSAKVYHVGSGTS
                     GSRYNLFKIRYSSRNNIYLIYKNMPVLQILLNLPVFILGFSAKLVFFASKGYGKEYLA
                     GIKNGFFICRKPENRAKKIRFEWKRLGTYFVIEWELFINVFRRFLERA"
     gene            7162..8571
                     /locus_tag="PLN77_01920"
     CDS             7162..8571
                     /locus_tag="PLN77_01920"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRM43073.1"
                     /translation="MINDNQKYFDRMHVLIDALVLALSYVLSWYCYVRLGVMNNHPER
                     GILPPATYMMALLYIVPGYLLLYYAFNLYASKRFQGRRLEFANILKANTVGFLVWLAS
                     LYMVRQVHFSRGMLCIFFVLNVTLETIVRNLIHYILSNMWKHGRNQKQILMVGYSRAT
                     EEYIDRIIANPEWGYLVRGILDDHVKAGTMYRGVKVLGRINNLSVILPENKLDEIAIT
                     LGLNEYFRLEEIVAMCEKSGVHTQFVPDYHNIIPTKPYTEDLQGLPVINIRYVPLSNT
                     FNAMLKRIVDIFGALVAIILFSPVMLFSCIMIKLTSPGPLIYRQTRVGLHNKPFEMYK
                     FRSMEVQTEEKEKKAWTVKNDPRVTGFGKFMRHTSIDELPQLFNILKGDMSLVGPRPE
                     RPFFVEKFREEIPRYMVKHQVRPGLTGWAQVNGYRGDTSIRKRIDCDLYYIENWTLGL
                     DFKILFLTFFKGFVNKNAY"
     gene            8590..9786
                     /locus_tag="PLN77_01925"
     CDS             8590..9786
                     /locus_tag="PLN77_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRM43074.1"
                     /translation="MSKKNYKKSRKYKKMRRRKIIFGIEITVLLILSGILFVYAWINR
                     SMDKMNQDTLDSSQIQINSEVKANTDLSQMSGTQVIALVGVDARGVEGSELAESMNSD
                     TIILCCIDHDKQEIRMVSIMRDTWMNMAKYTDEYYEFDKANSAYNRGGPESMLSMLNT
                     NLDFALTDYVTVNFKALADAIDVLGGLDIEMTNAECVHANNYNREVSEAQGVEYEAIP
                     YDEDLGDDYSEVRHVSGALATSYARIRYGGGDDAKRTSRQRIVINLMVQKLKQNPTKI
                     PEILDKVMGNVSTSLTKNEILELGMHAVTYTMGTSYAYPFQLCYGENVVNALGEDVVI
                     PVTLEFNVRELHEYLYPGLSYEPSAAVTEYSDYIARKSGYDEDMIGYVLNQGPGAEAD
                     SVIAGD"
     assembly_gap    9829..9852
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9853..10764
                     /locus_tag="PLN77_01930"
     CDS             <9853..10764
                     /locus_tag="PLN77_01930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM43075.1"
                     /translation="PGQEFAELLPRLLEQTYPIRSIRVVNTGKKFWNPAWEQLSDKIM
                     VEHIRPSEFDHGGTRRAMAESSDADLLLFMTQDAMPYDNECVEHLVEKFSLPHVKAAY
                     ARQLPRDDCRTLERFTRGFNYPEESDIKDAGDLPKLGVKTFFCSNVCAMYDRRTYEEL
                     GGFVESTLFNEDMIYAGHLVQAGYAIAYAADAKVVHSHNYGCLAQFHRNFDLGVSQAQ
                     HPEIFDAYPSEGEGIRMVKKSASYVCKIHKPWLLVTLFFQSGFKYVGYFLGKRYKSLP
                     DGVIRWCTSNKTWWEYHGKIRKKRNRR"
     gene            10938..12764
                     /gene="glmS"
                     /locus_tag="PLN77_01935"
     CDS             10938..12764
                     /gene="glmS"
                     /locus_tag="PLN77_01935"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRM43076.1"
                     /translation="MCGIVGYIGTCQAAPVLLDGLSKLEYRGYDSAGIAVYDGNKIQM
                     QKAMGRLKVLEEMTQNGATLPGTVGIGHTRWATHGAPSDLNAHPHFNKDHSIVVVHNG
                     IIENYLKLKKKLMSKGYEFLSETDTEVIAHMLDYYYNGDPLATITKVMHRMEGSYALG
                     ILFRDHPDEVYAVRKDSPLIVGTSKSGNLIASDVPAVLKYTRDVYFLENEEIVKLTRD
                     GLHFYNIDEEELEKEATHIDWDMNAAEKGGYEHFMLKEMHEQPKAVKDTLTPRIKNGD
                     VVIEELGMTDEEIRAIRKIFIVACGSAYHTGVTSKYIFEKLARIPVEVDLASEFRYRD
                     PILPENTLVVIVSQSGETADTLAALRLAKEKGVRTLGIVNVVGSSIAREADNVMYTWA
                     GPEIAVATTKAYSTQLIAQYLLAMKFAKVRGTVSQNDFDAMIQSLERLPEQISLLLSH
                     KENVQRFANRYLAAEHVFFIGRGIDYAISMEGSLKLKEISYIHSEAYAAGELKHGTIS
                     LIEDGRLVVAVLTQPELYKKTISNIQEVKTRGAFVMAVTTVGNTEVEKVADYVVYLPE
                     TNPIFANSLAIIPLQLFGYYVSIGRGLDVDKPRNLAKSVTVE"
     gene            12910..14631
                     /locus_tag="PLN77_01940"
     CDS             12910..14631
                     /locus_tag="PLN77_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRM43077.1"
                     /translation="MSEEFKKEEINGEAAEETKAAEHTEEVKAETTAEQSAAETEKPQ
                     ETAEAPKTEEKKTTTYSWVNPKISGKTGEENNSASYTGKPDPSEAKAERTSSAKTEHY
                     QYQSQTQSAPKQEKPQFSAVPQPQDHKKKAKKPMSSAKKWGMTLAMAACFGLVAGGVF
                     IGTAAVGTKVIGTATEQKQEVTIPTTTTTTAADSSDTSDTVKATGEMSVKDVASSAMP
                     SLVAISTTTVEEVETFFGTTSQEVPASGTGVIVGQNDDELLIATNNHVVSGATSLSVS
                     FIDDETVEGQIKGTDADNDLAVVAVKLSDIKDETKSQIKIAVMGNSDDLEVGDQVVAI
                     GNALGTGQSLTSGYISAKDRTISSQDESTGETITSEGLIQTDAAINPGNSGGALLNMN
                     GELIGINEAKYSSTQVEGMGFAIPISKAEPILQNLMNLTTRYKVSDDEAAYIGINMAD
                     VSADVSQNYGIPTGVCIMSVVDGSPAADAGFKKGDVITTFDGRSISNAKGLKETLTYY
                     AAGETVDVTVQRADNGEYKEVTLTLTLGSAADMPQTSGSSNGTTNDGTGSSDQNDQGI
                     QNPFGNR"
     gene            14857..16293
                     /gene="clpX"
                     /locus_tag="PLN77_01945"
     CDS             14857..16293
                     /gene="clpX"
                     /locus_tag="PLN77_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRM43078.1"
                     /translation="MSEEKDRVDGTADETKAETKSSDHHTEDTYEKVCFMCRRPESKA
                     GKMITLPGNINICPDCMQKSFDTMNQNPGQINEMMNNMNNMPNIGMFDLSGLSNQIPN
                     RQRVKKKEEKKKTKEFSIKDIPPPHRIKAMLDEYVIGQDRAKKVMSVAVYNHYKRVFA
                     PQDGNIEIEKSNMLMIGPTGSGKTYLVQTLARLLDVPLAITDATSLTEAGYIGDDIES
                     VVSKLLAAADNDVERAEHGIIFIDEIDKIAKKKNTNQRDVSGEAVQQGMLKLLEGSDI
                     EVPVGANSKNAMVPLETVNTRNILFICGGAFPDLTDIIKERLNKTASIGFQADLKDKY
                     DGDKNLLSQVTLEDLRTFGMVPEFLGRLPIVFTLESLNEDMLVEILKEPKNAILKQYK
                     RLLELDEVKLEFDDDALRSIARKALEKDTGARALRSIIEDFMLDIMYEIPKDSSIGEV
                     IITKDYIEKTGGPTILLRGQEVPKLEQK"
     gene            16374..16865
                     /locus_tag="PLN77_01950"
     CDS             16374..16865
                     /locus_tag="PLN77_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRM43079.1"
                     /translation="MAEDFFGNLSKTLKKTVDTVGKKTDEFVEIQKIRTRQHALEDQI
                     EKNYQDIGQIIYNRYLNGEAFDENLAGICKDITDLEKEIADCREDVANKRGRIVCPAC
                     GASVPKDAAFCMRCGAAMPEKEEEPEDAEFVHVEPEEKAEETEAAAPEEEPEKEAEVP
                     EEE"
     gene            16862..17320
                     /locus_tag="PLN77_01955"
     CDS             16862..17320
                     /locus_tag="PLN77_01955"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRM43080.1"
                     /translation="MIWTLLPGAVFLADGCLKKKAEEELKENEQIPVCKGHIVLRKCH
                     NRGVAGGGFADSTKKVEILTFFLLSGLWARLGALAGRRKCKAKKLGLALTIGGGMSNW
                     YDRHKRGYVTDYVSFRFGPEKFRRLVFNVSDFCIFFGIALLTAISCRESR"
     gene            17483..17566
                     /locus_tag="PLN77_01960"
     tRNA            17483..17566
                     /locus_tag="PLN77_01960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17517..17519,aa:Leu,seq:cag)
     gene            17868..17951
                     /locus_tag="PLN77_01965"
     tRNA            17868..17951
                     /locus_tag="PLN77_01965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17902..17904,aa:Leu,seq:cag)
     gene            18253..19326
                     /locus_tag="PLN77_01970"
     CDS             18253..19326
                     /locus_tag="PLN77_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HRM43081.1"
                     /translation="MKAFLILEDGHVFTGTSIGSQKEVISEIVFNTSMTGYLEVLTDP
                     SYAGQAVCMTYPLIGNYGICYDDQESLRPWPDGYIVRELSRLPSNFRCQDTIQNFLKK
                     FDIPGIAGIDTRALTKILREKGTMNGMITTNENYHLDEIIPKLKAYNTGKVVEKVTCS
                     EKSVLKGEGFKVALLDLGAKNNIARSLNERGCEVTIYPALTTAEEILAANPDGIMLSN
                     GPGDPKECVSIIKEIKKLYDSNVPIFAICLGHQLMALATGADTHKMKYGHRGGNHPVK
                     DLATGRVYISSQNHGYVVDTDTLDPNVAVPAFVNVNDGTNEGLKYVGKNIFTVQFHPE
                     ACPGPQDSGYLFDRFLEMMGGNK"
     gene            19326..>22101
                     /gene="carB"
                     /locus_tag="PLN77_01975"
     CDS             19326..>22101
                     /gene="carB"
                     /locus_tag="PLN77_01975"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRM43082.1"
                     /translation="MPKNPNIKKVLVIGSGPIVIGQAAEFDYAGTQACRSLKEEGMEV
                     VLLNSNPATIMTDKDIADRVYIEPLTVEVVEQLIQKEKPDSVLPTLGGQAGLNLAMEL
                     EEKGFLKENNVRLIGTTAQTIKKAEDRQEFKDTMEKIGEPVAASLVVHDVQAGIDFTN
                     KIGYPVVLRPAYTLGGSGGGIAYNEEELIEILSNGLRLSRVGEVLVERCIAGWKEIEY
                     EVMRDSVGNCITVCNMENIDPVGVHTGDSIVVAPSQTLGDKEYQMLRTSALNIITELG
                     ITGGCNVQYALNPDSFEYCVIEVNPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     PNAITGKTYASFEPMLDYCVVKIPRLPFDKFISAKRTLTTQMKATGEVMSICNNFEGA
                     LMKAIRSLEQHVDSLMSYDFSHLSKDELIEELHIVDDMRIWRIAEAIRQGISYDEIHE
                     ITKIDVWFIDKLAILVEMEQALQAKELDEDLLREAKRLEFPDYLVAKLAGKTEEEVKA
                     LRKQYDITAAYKMVDTCAAEFAATTPYYYSVYGGENEAVETNDRKKVLVLGSGPIRIG
                     QGIEFDFCSVHCTWAFKKEGYETIIINNNPETVSTDFDIADKLYFEPLTPEDVENIVN
                     IEHPDGAVVQFGGQTAIKLTEALLKMGVPILGTSAENVDAAEDRELFDEILEQCEIPR
                     PKGHTVFTAEEAKKAANELGYPVLVRPSYVLGGQGMQIAISDEDVDEFIGIINRIAQE
                     HPILVDKYLQGKEIEVDAVCDGTDILIPGIMEHIERAGVHSGDSISVYPAQSISQHAK
                     DTIVEYTKRLARSLHVIGMINIQFIVCGEDVYVIEVNPRSSRTVPYISKVTGIPIVPL
                     ATKVILGHTIRELGYEPGLQREADYIAIKMPVFSFEKIRGADIALGPEMKSTGECLGI
                     AKTFNEALYKA"
BASE COUNT         6302 a         4913 c         5874 g         4988 t
ORIGIN      
        1 tctgattttc agccgcaggg acgatacagc atgccgatgc tgattccgct gatgtatttc
       61 gtgacgaagg gtgtgcaggc gttcctgaaa cgggagaaaa aactacagaa gtatgaaaaa
      121 ttgttctgta ttctggtctg tgtggcggca gcggcattag cggcattcac ttgggcgaga
      181 atcatctatc cgatgtactt gcagaactac tacggattgc ggtaagcgga acagagaaag
      241 aaagcggtga aacgtttgga aaaaatttca gtgctgacag caaaattaaa aaaagctgtt
      301 gcagaagaag gaatcggcgg tctggctggg cgtacgaaag ggtaccttgc gcgggcaaaa
      361 aaagaaaaag agtttcagag agaaaaatcc cgggtctacc gggatattct ctttatcagt
      421 ggctgcaatg aacagctgcc gcatccacac cggtaccgcg tggtgcatca gatggagcag
      481 ctggaggcag gcggatatac gtgtgatacg gtttattttc aggagctgaa gccctggatg
      541 gtacgctgct acggcgcgtt cgtgattttc cgctgcccga tgacggacac cctgcgggaa
      601 tttgccacaa tggcaaagca gatgaacaag cctctgtggt acgatgtgga tgatcttgtg
      661 atcgatacga aatacaccga tcagattccg tttctcgacc ggatgcagcc ggaagaacgg
      721 caggcatacg atcagaatgt ccgaaacatg ggcgagctgc tttccctctg tgatgcggcg
      781 gttacgacga cagcggcgct ggcagaagaa ctgaagcagt acgtaccgga ggtgctgatc
      841 aaccgcaact gtgcgtcaga tgagatgctg ctactgtccg aggcggtgtt aaaagaaaaa
      901 cagaaaaaga acgaagcaga tggaacggcg aaaaaagtac gccttggcta tttcagcggc
      961 agtgcgacgc atctggacga catcgagatg atcgttccgg tgctgaaaca gctgcttggc
     1021 aaaaatccga acctggaact cctcatcgtg ggaattctgg aactcccggt ggagctgaaa
     1081 ctgtttgctt cccagatcca gatggaggga tttgtcgatt accaaaaact gccggagcgg
     1141 attgcgtcgg tggatatcaa cttggctccg cttaccgata cgatttttaa ccgtgcgaaa
     1201 tcggaaaata aatgggtgga agcggctctg gtgcagaccg tgacagcagc cagcaatctc
     1261 ggcgcttttg ctgagatggt acaggatgga gaagacggcg tactctgccg agacgaagcg
     1321 gagtggttgg aaaaactgca gtggctgatc gacgacgaac cggcacgaaa agccattgcc
     1381 ggacgggcat atggccggtg cagccgcgag tgtgtcacaa tctttcatgc caccgggatt
     1441 tgcgagtggg tggagcgcca ctggaatcta cgctgtgcct ttgtgcttcc agcgatggag
     1501 atcagcggag gcatccgcgt tgcacttctt catgcggaaa tgctggtaaa agcaggagcg
     1561 caggtcagcc tctttactct ggaaggggag gcggagtggt atcatgaggg tgatttccat
     1621 tttccggtgc tgtcggccga gcgagagaaa ctgcagggaa cgctggatct tgcggtagcg
     1681 acgatgtgga ataccgcaga gttcgtcgaa cagtccagta agatccgaaa gaaaaaatat
     1741 ctggtgcaga attttgaagt tggattttat ccgccgggaa gcccgtaccg gatcgcaact
     1801 tcggcgactt accggatgag gagcccgatg gagtacgtaa ccatttccaa atggtgtcag
     1861 aactggctgc gggaggaata ccataccgaa gcagtctatc ttccgaacgg catcgatccg
     1921 tccttctatc cgaaacgggg gcgggatctg cagggaaaaa ttcggattct gatcgaggga
     1981 gactgcagcg cggagcataa aaacgtggac gagagcttcc gtatcgtgga acagctggat
     2041 ctggaaaaat tcgagatctg gtacatgtcc tacaatggaa atccgaaatc ctggtaccgc
     2101 gtggaccgct ttttacaccg ggttccgtat gaaaaaacac cggaagtcta tgcggcgtgt
     2161 gatatcctgc tgaaaacaag cctgctggag agcttttcct atccgccgct tgagatgatg
     2221 gcgtccggcg gctacgtcgt tgctgtgccg aacggtggca atctggaata cctgaaagat
     2281 ggggaaaact gtattctcta tccgcaggga aatctcgcgg aggcaaaagc ggcgattgag
     2341 cgtattctga cagatgcgga actgaggaaa aaactggata cgggagcaga agagacggta
     2401 aaggaaagaa actggaaaag aatcgaacca cagattttag aacagtatct tggaaaataa
     2461 ataagcaaca aaaagggctg tcgcactagc aaatatttct ggcggtagct ctgctttata
     2521 cccaaatggc atcgaaatcg gttgcgtttt cgatgccatt ctttacttct tataggaaag
     2581 ttcgacttcc gtcgaactct tgaatcagga tcacccgttg gacgggaggt cctgattgct
     2641 tataaattag aagatcagtg ccgtttgttt cggttattgc ggcgtctgtt tctgcgtttc
     2701 cgtctctttc tcgcgttgtt gatcacgaca atgatcagaa tcagaaaagc gacaccgttc
     2761 aggccaagca gaatatagag cagtgtggag agaaaagaaa ttttcttggt ttcttccggt
     2821 gttggaatat ctgtgctgtc ggtttcttct gatgaatcgg aggaaaccga agaactgtcg
     2881 gaaacagtac ctggagcgga aacctcggct gttccgatga agtgatcatc gaaaaaatac
     2941 tggcgggtgt acggttcctc aacggaagcg ccgtccggat cggtgtaggt tgcagtttca
     3001 attttcgcat tctttggaag cgttaccaca ccggaaagaa tttccttttt tgcgttcttc
     3061 agggtataat gtgtaaaatt atcataaccg aaatcaagta cggtacgggt atcgttgata
     3121 taatcgaggt tatcacaggt taaaacaacg cagaccagcg tcatattgcc tcgcttggca
     3181 gcggtaacga gtgtgttgtg tgcctcatcg gtataaccgg tctttccggc gaaagcaccc
     3241 ttgtatcccc actgaccgtc ctggaacagc aatgcgtggt ggttctgaag atcacgcggt
     3301 gtcgaggtca tattggtcgg ctgaattgtg taggtcagat cggactcgat gcggcagaag
     3361 gtctcgtttt tgatgcactc ctgcataatg agtgccatat cgtgtgcact ggtgtagtgg
     3421 ttcggatctg gcagaccgtt tgcattgaca aaatgggtat tcgtgcaacc aagtgcagcg
     3481 gcgcgttcgt tcatcatggc ggcgaaacct tcctcgcttc cggcaatgtc gatggcaagc
     3541 tgtgtgcaga cctcatttgc cgaggcaaga agaatcgcgt gaagagcatc ctccactgtg
     3601 atcgtttcgc caagctgaac gttgatgcgg gcatttcccg gaatggattc gctgacagca
     3661 tattcgccga aggtaacggt gtcggtcatc tgcttgtttt caatgatcag cagagccgtc
     3721 agaatcttgg tgatgctggc aggatagcgc tgctcgtcca tgtttttatc gtataaaaca
     3781 gccccagtat cggcatcgag taaacagaca gcgccggaat tgttttccgg tccttcgggc
     3841 caggtggtga ctgttttttt atcggttgtg gcaaaaatcg tctgcgggag gcaggacgcg
     3901 gcaaaaacaa aagcaagcaa caggcataaa aatgtgcggg ctgcagaatt ctttctaatc
     3961 ataactcact ccttctgatc cagcgggtac agctgcataa gactgtccgg gcagcggatc
     4021 tctctaaaat gtattagaca cacttttatt attataacat aaagcgagca gggaagaaac
     4081 acataatttc ttaatatttt cagtcgggat ttgcaaaatg cagtaaaata gagtataatg
     4141 cgaacgagtt catacggaaa aaacaaaaaa cctgaaaaac aaaaacccca aggagttaaa
     4201 aaaagtgtca gatatgaaat ggctccgttt tatacggaaa ttgaaacctt acaacataca
     4261 gaagggactg cgttacctga aacattatgg gccaaaggaa ttcctgatcc gtctttcgga
     4321 gcggatggag ccggaggagg ttccgtacgg accgtggcgg gagcagtact gcgcaaaaga
     4381 ggaggacctg gcgcgtcaga aaaatcataa attttcttat tgtccgaaga tcagcgttgt
     4441 tgttccggct taccgtacgc cggaaatttt tctgcgtcag atgatcgaat ccctggaaaa
     4501 acagagttat gaaaactggg aactggtgat cggaaatgcc agcccggagg atgagactat
     4561 ggcgcgcatc ctgaaagagt ataccggaaa agatccgcgc gtaaagaacg ttccgattcc
     4621 ggaaaatatc ggcattgcag agaatacaaa tgcagcgctt gccgcagcag cgggaaattt
     4681 cgtcggtttt atggatcacg acgatctgct tgcgccggat gcactctttg aaattgccat
     4741 ccgtctggaa aaagatccgt ccatcgatgc tttttatacc gatgaggaca aggtacggac
     4801 cgatctgtcc gagtatttcc agccgcattt caaaccggat ttcaatctgg atctgctgcg
     4861 gtcaaacaat tatatctgcc atttcttcgt tgtacggcgg gagattgcgg agaaaaccgg
     4921 cggccttcgt ccggaataca atggggcgca ggattatgat tatatcttcc gctgcacgga
     4981 aatggcggga aaaattgtac acattccgag ggttttatat cactggaggg tccacagcgc
     5041 ttccaccgca gacaatccgg caagcaagct gtacgcgtat gaggccggaa aaaaggcaat
     5101 tgagggaaac cttgcgcgct gcggcgaaga gggaaccgtc acactgcgtt cggattatgg
     5161 cttctacgat gtagattata aactgcgggg aacgcctctg gtttcgattc tgatcccgaa
     5221 caaggatcag gccgatacgt tacgcacttg tctggaatcc attaagaaga agtgcatgta
     5281 tccatcctat gagattctga ttattgagaa caacagcact gaggaagcaa cgttttccta
     5341 ttacaaagaa ctcgaagcac agggcatccg tgtcctgaag tatccaaaac agggctttaa
     5401 ctattctgcg atcaataatt tcggcgtcaa agaggcaaag ggagagtatc tcctgtttct
     5461 caataacgat atggaaatca tcacacccga ttttatagaa aaaatggtgt ccaatctgca
     5521 aagaccgcag atcggggcgg tcggcgcgaa gctgtattac ccggacaaca cggtgcagca
     5581 tgcgggtatc atcatcggta tcggcggcat tgcgggccac gcctttttag gactggcgcg
     5641 cggaagaagc ggctacctgc ataaggcatc gctccagatg aatgtgagtg cggtgacagc
     5701 agcgtgcatg atgatgagaa aagaggtctt tgaggaggtc ggcggctttg aagaagaact
     5761 gtccgtcgct ttcaatgatg ttgatctgtg cctgcgcatt ggaaaagcag gttataaaat
     5821 cgtatacaat ccgcacgtgg agttgtacca ttatgaatcg aaatccaggg gagcagagga
     5881 tgacgagaag aaggtgcgcc gcttccagtc ggaaatcgaa tttatgcgca gccgctggat
     5941 tgggctgtta aaggcaggag atccctgcta caatccaaat ttaacgctgg catcgtggaa
     6001 ctatggactg cgtgccgacg gcaggggagt aaaaccatgg ataaagtagc agttgtaata
     6061 ccaaattata atggataccg gtatctggag acgtgctttg cagcgcttgc aaagcagacc
     6121 tttacggaat ttaccacata tttcgtggac aatggttcac aggatgagag ctgtgcgttt
     6181 ctgcgggaac actacccgga gacgaaaatc atccagctcg atgataacta tggcttcagc
     6241 cgtgcggtca acgaaggcat ccgccagtcg aaggagccgt atgtgatcct tttaaacaac
     6301 gacaccgaag tgttcccgga ctttgtcgag cgcctttatg agggaatctg taaaagaaag
     6361 aatgcgtttg catgcagcgc aaagctgatt tcctatcagg agcgtgataa aatcgacgat
     6421 gcgggaaatt actataatat gctgggatgg gcattcgcaa gaggaaaagg aaaaccggtc
     6481 agtgaatttg aaacgtccga ccggattttt gcggcctgcg ccggagcagc gatctaccgc
     6541 cgggaatttc tggaaaagac cggacttttt gacgaagaac atttcgctta tctggaagat
     6601 accgacatcg gctatcgtgc gcggcttttg ggctacgaga actggtatga gccgtccgcg
     6661 aaggtttacc acgtcggaag cggaacgagc ggctcccggt ataacctctt taaaatccgt
     6721 tattcttcca ggaacaatat ttatctgatc tataaaaata tgccggtttt acagattttg
     6781 cttaacctgc cggtctttat cctcggtttt tcggcaaaac tggtcttttt tgcatcgaag
     6841 ggatacggaa aagaatatct ggcaggaatc aagaatggtt ttttcatctg ccggaagccg
     6901 gaaaacagag caaaaaagat ccgtttcgag tggaagcgcc tcggcacgta ttttgtcatc
     6961 gaatgggagc tttttatcaa tgtgttccgg agatttctgg agagggcata aggattcttt
     7021 caaaagatta cgaaaaagtt aaaaagttta cggaaataag aagaatgtta gaaaaatatg
     7081 attgctcttt tttggaactt attgtatagt taggggtagg gaatttttaa atcccgaaaa
     7141 aaacagataa ggtgaacgaa tttgatcaac gataatcaga aatattttga ccggatgcac
     7201 gttctgatcg atgccctggt gctcgcactt tcctatgtac tgtcctggta ctgctatgtg
     7261 cggctgggcg ttatgaataa tcatccggag aggggaattc tgccgccggc gacgtatatg
     7321 atggcactgc tctatatcgt gcccggctat ctgctgcttt actatgcatt caatctctat
     7381 gcatccaagc gttttcaggg aagacgtctg gaatttgcca atatcttaaa agcgaatacg
     7441 gttggatttc tggtctggct ggccagcctt tacatggtgc gccaggttca cttttcccgt
     7501 ggtatgctct gcatcttctt tgtgctgaat gtgacactgg aaacgatcgt aagaaatctg
     7561 atccactata tcttaagcaa tatgtggaaa cacggccgca accagaagca gattctgatg
     7621 gtcggctaca gccgtgcgac cgaggaatac attgaccgga tcattgcaaa cccggagtgg
     7681 ggctatctcg tccgcggaat cctcgatgac catgtcaaag cgggaacgat gtaccgcggt
     7741 gtcaaggttc ttggaagaat caacaatctt tcagtcattc tgccggaaaa caaactggat
     7801 gaaattgcca ttacccttgg actgaacgaa tacttccgcc tggaggagat cgttgccatg
     7861 tgtgaaaaat ccggcgtaca tacccagttt gtgccggatt accacaacat cattccgaca
     7921 aagccgtaca cagaagacct gcagggactt cctgtcatca atatccgcta tgtgccgctt
     7981 tccaacacgt tcaacgcgat gctgaaacgg attgtggata ttttcggcgc gctcgtggcg
     8041 atcattctgt tctcaccggt gatgctgttt tcctgcatca tgatcaagct gacctcaccg
     8101 ggtccgctga tctaccgtca gacacgcgtc ggactgcaca acaaaccgtt tgaaatgtac
     8161 aagttccgtt cgatggaagt tcagacggag gagaaagaaa agaaggcatg gaccgtgaag
     8221 aatgacccgc gtgtgacggg atttggaaaa ttcatgcgcc ataccagcat tgatgagctt
     8281 ccacagctct ttaacatctt aaaaggagat atgagcctgg tcggaccgcg gccggagcga
     8341 cctttctttg tggaaaaatt ccgggaggag atcccgcgtt atatggtaaa acatcaggtt
     8401 cgtcccggtc ttacgggatg ggcgcaggtg aatggatacc ggggggatac ttctatccgc
     8461 aagcgtattg actgtgatct gtattatatt gaaaactgga cgctgggact tgattttaaa
     8521 attctgttcc tgaccttttt caaaggattt gtgaataaaa acgcttacta aaagataagg
     8581 ggaaaagaga tgtcgaagaa gaattacaaa aagagcagaa aatacaaaaa aatgcgccgc
     8641 agaaaaataa ttttcggaat tgaaatcacc gttctgctga ttctttccgg aattctgttc
     8701 gtctacgcat ggatcaaccg ttcgatggat aagatgaatc aggatacgct ggacagctcc
     8761 cagattcaga tcaacagtga agtaaaggca aataccgatc tctcacagat gtccggtaca
     8821 caggtcatcg cgctggtcgg cgtggatgcc agaggtgtgg aaggctccga gctggcagaa
     8881 agcatgaaca gtgacacgat tattctctgt tgtattgatc atgacaagca ggaaatccgt
     8941 atggtgtcga ttatgcgtga cacctggatg aatatggcga aatataccga tgaatattat
     9001 gagtttgaca aagccaattc cgcatacaac cgcggcggcc cggagagtat gctctccatg
     9061 ctgaatacaa accttgactt tgcactgacc gattacgtta ccgtaaactt caaggcactc
     9121 gccgatgcga ttgatgtcct gggcggtctc gacatcgaga tgacaaatgc agagtgtgtt
     9181 catgcaaaca actacaacag agaggtatcg gaagcacagg gcgtagagta tgaagctatt
     9241 ccgtatgatg aggaccttgg ggatgattac agcgaagtac gtcatgtcag cggtgcgctt
     9301 gctacctcct atgcgcggat ccgttatggc ggcggtgatg atgcaaaacg taccagccgt
     9361 cagcgaattg ttatcaatct tatggtacag aagctgaaac agaatccgac caagattccg
     9421 gaaattctgg acaaggtgat gggaaatgtc agcaccagtc ttaccaagaa tgaaatcctg
     9481 gaacttggta tgcatgcggt aacgtatacg atgggaacct cctatgccta tccgttccag
     9541 ctctgctatg gtgaaaatgt agtgaatgcc cttggtgagg atgttgtaat tccggttacg
     9601 ctggagttca atgtaagaga gctgcatgaa tatctgtatc cgggtctttc gtatgaaccg
     9661 tctgcggctg ttacagagta cagcgactac attgcaagaa agagcggtta cgatgaagat
     9721 atgatcggct atgtgctgaa ccaggggcct ggtgcagagg cagattcggt gatcgcaggc
     9781 gactaaatac gagggagaaa accagacatg aaattctata ccgttgatnn nnnnnnnnnn
     9841 nnnnnnnnnn nnccagggca ggagttcgca gagcttctgc cccgcctttt ggaacagact
     9901 tacccgatcc ggtcgatccg ggttgtaaac acagggaaaa agttctggaa cccggcgtgg
     9961 gagcagttat cggataaaat tatggtagag catatccggc caagcgaatt tgaccacggc
    10021 ggaacgagaa gggcgatggc agaaagttcg gatgcggatc ttctgctttt tatgacccag
    10081 gatgccatgc cgtacgataa cgagtgcgtg gaacatctgg tggaaaaatt ttcactgccg
    10141 catgtgaagg cggcctatgc gcggcagctt ccgcgggatg actgccgtac actggaacgg
    10201 tttacgagag gctttaacta tccggaggag agtgatatca aggatgccgg tgatctgcca
    10261 aaacttggtg ttaagacttt tttctgctcc aatgtctgtg caatgtatga ccgcaggaca
    10321 tatgaagagc tgggtggatt tgtagaaagc accttgttta acgaagatat gatctatgcc
    10381 gggcatcttg tgcaggcggg ctatgcgatt gcctatgcgg cagatgcgaa ggtggtacat
    10441 tcccacaatt acggttgtct ggcacagttt caccgcaatt tcgacctcgg tgtatcccag
    10501 gcacagcatc cggagatctt tgatgcgtat ccgtctgagg gtgagggaat ccgtatggtg
    10561 aaaaaatcgg catcctacgt ctgcaaaatt cataagccgt ggctgctcgt cacgttattc
    10621 tttcaaagcg gattcaaata tgtgggatac ttcctcggaa aacgatacaa aagccttccg
    10681 gacggtgtga tccgctggtg tacctcaaac aaaacgtggt gggaatatca cggaaagata
    10741 agaaaaaaac gaaatagaag ataagtgaaa gtaaaaaaca aaaataaaaa gattttgtaa
    10801 gtaaatacga aatgctccat aaggttgaca gagaacggag cagcgtggta aaaaagtaaa
    10861 gaacaagaaa ttagaaggcg gcataacctg aaaacagaac aagggtgccg ccttttgctg
    10921 ttaggaggaa aaaatctatg tgtggaattg ttggatatat tggaacctgc caggctgccc
    10981 cggtgctgct ggacggattg tcaaagctgg agtaccgcgg atacgattcg gccggaattg
    11041 ctgtatacga tggaaacaaa attcagatgc agaaagcaat gggccgtctg aaggtgcttg
    11101 aggaaatgac acaaaatgga gcgacacttc cgggtaccgt aggaatcgga catacgagat
    11161 gggcaacgca tggggcacct tccgatctga atgcacatcc gcatttcaat aaagaccact
    11221 cgattgtcgt tgtacacaat ggaattattg agaattatct gaaattaaag aaaaaactga
    11281 tgtccaaagg atatgagttc ctttcggaga cagataccga ggtaattgcc catatgctgg
    11341 attactatta taacggcgat ccgcttgcaa cgatcacaaa ggttatgcac cgcatggagg
    11401 gatcgtatgc actgggtatc ctgttccgcg accatccgga tgaggtgtat gcggtccgta
    11461 aagacagccc gctgatcgtt ggtacaagta agagcggaaa cctgattgca tccgacgttc
    11521 cggctgtcct gaaatataca agagatgtct attttctgga aaatgaggag atcgtgaaac
    11581 tgacccgcga tggccttcac ttctacaata ttgacgagga ggagctggag aaagaggcaa
    11641 cccacatcga ctgggatatg aatgcggcag aaaaaggcgg ctatgagcat ttcatgctga
    11701 aagagatgca tgagcagcca aaagccgtaa aagataccct gacgccgcgt attaagaacg
    11761 gtgatgttgt gatcgaagaa ctcggcatga ctgatgagga gatccgcgcg atccgcaaaa
    11821 tctttatcgt tgcctgcggt tccgcatacc acaccggtgt aacctcaaaa tacatcttcg
    11881 aaaagctggc aagaatcccg gttgaggtag atctggcaag cgaattccgc taccgtgatc
    11941 cgattctgcc ggaaaatacg ctcgtggtca tcgtcagcca gtcgggtgag acggccgata
    12001 ccctcgcggc tctccgcctt gcaaaagaaa agggcgtgcg tacccttggc atcgtaaacg
    12061 tagttggaag ttcgattgca agagaggcag acaatgtcat gtacacctgg gccggtccgg
    12121 agattgctgt tgcgacaacg aaagcgtaca gcacacagct tatcgcgcag taccttctgg
    12181 cgatgaagtt tgccaaagta cgcggaaccg tttcgcagaa tgacttcgat gcgatgatcc
    12241 agagccttga gcgcctgccg gagcagattt ccctgctgct gtcccacaag gagaacgtac
    12301 agcgttttgc caaccgttat cttgcggcag aacacgtctt tttcatcgga cgcggcatcg
    12361 actatgcaat ctcgatggag ggctccctga agctgaaaga gatttcctac atccattccg
    12421 aggcatatgc agccggtgag ctgaaacacg gaaccatttc cttgattgag gacggacgcc
    12481 ttgtcgtagc cgtgctgacc cagccggagc tctacaagaa aacgatcagc aatattcagg
    12541 aagtgaagac acgaggcgcg tttgtcatgg cggtaacgac cgttggcaat acagaagtgg
    12601 aaaaagtagc cgactacgta gtttacctgc cggagacaaa tccgattttt gcaaactccc
    12661 tggcgatcat tccgctgcag ctctttggct actacgtatc gatcggcaga ggcctcgatg
    12721 tggataaacc gagaaacttg gcgaaatcgg ttacggtaga ataaaaaccg cagcataaaa
    12781 ttcctctttt ttcaaaatta tcacagtttc aacacaattc taacgtacaa taggcagtag
    12841 aaaatgaaga aagaaataag agcactgtgt attggtgcct gagatagaaa agagaaagag
    12901 ggatcagcca tgagtgaaga attcaaaaaa gaagaaataa atggtgaggc agcagaggaa
    12961 accaaagctg cagagcatac agaagaagta aaagcagaaa cgacagcaga gcagagcgca
    13021 gcagagacgg aaaaaccgca ggaaaccgca gaagcgccga agacggaaga aaagaaaacg
    13081 acaacctaca gctgggtgaa tccgaaaatc agcggaaaga ccggcgagga gaataacagc
    13141 gcttcctaca ccggaaaacc ggacccgtcg gaggcaaagg cagagcggac atcttctgca
    13201 aagacagaac attatcagta ccagtcacag acacagagtg ctccgaagca ggaaaaacca
    13261 cagttttctg ccgttccgca gccgcaggat cacaaaaaga aagcgaaaaa accaatgtca
    13321 tccgcaaaaa aatggggcat gacacttgcc atggcagcgt gcttcggact cgtggcaggc
    13381 ggtgttttca ttgggactgc agcagtcggt acgaaagtga tcggtacagc aacagagcag
    13441 aagcaggaag ttacaattcc gacgaccacc acaacaacgg cagcagacag cagtgatacc
    13501 tccgataccg taaaagcaac cggcgagatg tccgtgaaag atgttgcatc gagtgcgatg
    13561 ccgtctctgg tagcaatttc tacgacaacg gtggaagaag ttgagacatt cttcggaacc
    13621 acaagccagg aagttcctgc aagcggaacc ggagttattg tcggacagaa cgacgatgag
    13681 ctcctgattg cgacaaacaa ccacgttgtc agcggtgcaa cgtctctttc cgtcagcttt
    13741 atcgacgatg agacggtaga aggccagatc aaaggtaccg atgcggacaa tgaccttgct
    13801 gttgttgcag taaaactttc cgacatcaag gacgagacaa agagccagat caaaattgcc
    13861 gtgatgggaa attccgatga ccttgaggtc ggtgatcagg ttgtggcaat cggaaatgca
    13921 ctgggaaccg gccagtccct gacgagcgga tatatcagtg caaaagaccg tacgatctcc
    13981 agtcaggatg agagtaccgg cgagacgatt acttctgagg gactgatcca gaccgatgca
    14041 gccatcaacc ctggaaacag cggcggcgcc cttctgaaca tgaacggtga gctgatcggc
    14101 attaacgaag caaaatacag ctccacacag gtcgagggca tgggctttgc gatcccgatt
    14161 tcgaaagcgg agccgattct gcagaacctg atgaacctga caacaagata caaagtcagc
    14221 gatgatgagg ccgcttatat cggaattaac atggcggatg tcagcgcaga tgtttcccag
    14281 aactacggta ttccgaccgg tgtctgcatc atgagcgttg tggacggcag cccggcagcc
    14341 gatgcgggct tcaagaaagg cgatgttatc accacgtttg atggaagaag catctcgaac
    14401 gccaaaggct taaaagagac actgacctat tatgcggcag gcgagactgt agatgtcacc
    14461 gtacagcgcg cggacaacgg tgagtacaaa gaagtaacac tgacgctgac ccttggctct
    14521 gcagcagata tgccgcagac gagcggaagc agcaacggaa ccacaaacga tggaaccgga
    14581 agctccgatc agaatgacca gggtattcag aatccgtttg gcaacagata aaaactcgaa
    14641 ataactattt cctaagagaa aaaagacgga cagcacagga atatgaacgc ctggctgtcc
    14701 gcctttttct gcattttttg atataatttt ttgaatttgt aaaattctta aaaaagttgg
    14761 taatttttaa gaaagttttt acttgtgacc ctatggcttt tgggctataa tcaagctaga
    14821 ccaagcgatc tattacagaa aggaatttga aaaatcatgt cagaagagaa agacagagtg
    14881 gacggtaccg cggacgaaac gaaggcggaa acaaaaagct ctgaccatca tacagaagat
    14941 acctatgaaa aagtctgctt catgtgccgc agaccggaga gcaaagccgg aaagatgatt
    15001 acccttccgg gaaacatcaa tatctgcccg gactgcatgc agaagagctt tgataccatg
    15061 aaccagaacc cgggccagat caatgagatg atgaataata tgaacaacat gccgaatatc
    15121 ggcatgttcg atctctccgg gctttccaac cagatcccga accggcagcg ggtaaagaaa
    15181 aaagaagaaa agaagaaaac aaaagaattt tccatcaagg acattccgcc gccgcaccgg
    15241 atcaaggcga tgcttgacga atacgtcatc ggccaggacc gtgcaaagaa agtcatgtcg
    15301 gttgccgttt acaaccatta caaacgtgtc tttgcaccgc aggacggaaa tatcgagatc
    15361 gaaaaatcca acatgctgat gatcggaccg accggttccg gaaaaacgta tctggtgcag
    15421 acactggcac gcctcttaga tgtgccgctt gccatcacgg acgccacatc ccttaccgaa
    15481 gcgggttata tcggtgatga tattgaaagc gtggtatcta agcttttagc ggccgcagac
    15541 aacgatgtgg agcgcgcgga gcacggtatc atcttcattg acgagatcga caagattgcg
    15601 aaaaagaaaa atacaaacca gagagacgtc agcggagaag ccgtacagca gggaatgctg
    15661 aagctcctcg agggcagcga catcgaagtt ccggtgggag ccaacagcaa aaatgcgatg
    15721 gttccgctcg agaccgtcaa taccagaaac attctcttta tctgcggcgg tgccttcccg
    15781 gatctgacgg acatcatcaa ggagcgtctg aacaagacag cttccattgg cttccaggcg
    15841 gatctgaagg acaagtatga tggggataag aacctgctgt cacaggtgac cttagaagac
    15901 cttcggactt tcggtatggt gccggagttc cttggccgtc tgccgatcgt atttacgctg
    15961 gagagtctca atgaggatat gctggtcgag atcttaaaag aaccgaaaaa tgcgatcctg
    16021 aagcagtata agagactgct cgaactcgac gaggtgaaac tggaatttga tgatgatgca
    16081 cttcgctcta ttgcaagaaa agcgctggaa aaagataccg gtgcgagagc actccgttcg
    16141 atcatcgagg atttcatgct ggatatcatg tatgagattc caaaggacag cagcatcgga
    16201 gaggttatca tcacaaaaga ctatattgaa aagaccggcg ggccgacgat tctcctgcgc
    16261 ggtcaggaag ttccaaagtt agagcagaaa taaggtaact ggaaaacaca gcggagcaga
    16321 cgtgaaactg gcgttttctt gaaataagag ataagaaaga gagggtttta actatggcag
    16381 aggatttttt tggaaattta agtaaaacac tgaaaaaaac agtggatact gtgggaaaga
    16441 aaacagatga attcgtagaa attcagaaga tccgcacccg tcagcatgcg cttgaggatc
    16501 agattgagaa gaattaccag gatatcggac agatcatcta caaccgctat ctgaacggcg
    16561 aagcattcga cgagaatctt gccggcatct gcaaagatat caccgatctg gaaaaagaga
    16621 ttgcagactg cagggaagac gtggcaaaca agagaggccg catcgtatgc ccggcctgcg
    16681 gcgcaagtgt tccgaaagat gcagctttct gcatgcgctg cggtgctgct atgccggaga
    16741 aggaagaaga gccggaggat gccgagtttg tacacgtaga accggaagaa aaagcagaag
    16801 aaaccgaagc agcagcgccg gaagaagagc ctgaaaaaga agcagaggta ccggaagagg
    16861 aatgatctgg accctgctgc cgggagcggt tttccttgca gacggatgcc tgaaaaagaa
    16921 ggcagaagag gagttaaagg aaaatgagca gatcccggtc tgcaaaggac atattgtcct
    16981 gcggaaatgt cataaccgcg gcgtagcggg cggcggcttc gcggacagca cgaagaaggt
    17041 ggaaattctc accttctttt tgctgtccgg actgtgggcg cgtctcggag cgctggcggg
    17101 cagacgtaag tgtaaggcga agaagcttgg gcttgcgctt acgattggcg gcggcatgtc
    17161 gaactggtat gaccggcata agagaggtta cgtgaccgac tatgtcagct tccggtttgg
    17221 accggagaag ttccgcagac ttgtgttcaa tgtttctgat ttctgtattt tcttcggaat
    17281 tgcgctgctg acggcaattt cctgccggga gtccaggtga tgcaggatct cccgaaaagc
    17341 cctgcggatg gcgggctttt cggggcaaaa aagaaaattt gaaatttttt gaaaaaattt
    17401 gaaaaaagct gttgacagat tcggaatcat ctggtaatat atgttttgtt gagcggcaca
    17461 tgagagccgc aagacaagaa atgcaggagt ggcggaattg gcagacgcgc aggcttcagg
    17521 tgcctgtggt cgcaagatcg tgtgggttca agtcccatct cctgcattat tttttcgctt
    17581 tgcgtatcaa gaattggtcc ggtggaccaa ttcggattgc cctgatggga agtgtgaaaa
    17641 ctttccgtca gggtcttttt atttctatgc ggagaagatg tgcagacggg gaaagaaaaa
    17701 cagacgaatc tcagacagtg taaaattgtt taaaattatc gctattatcc atatttttca
    17761 taaaatcaaa aaaattaaaa ttttttcaaa aaaggtgttg acttttcttt agaaagctgt
    17821 tattatctat cttgtcaagc ggacaaccgc taagacaact aagaaatgca ggagtggcgg
    17881 aattggcaga cgcgcaggct tcaggtgcct gtggtcgcaa gatcgtgtgg gttcaagtcc
    17941 catctcctgc attatttttt cgctttgcgt atcaagaatt ggtccggtgg accaattcgg
    18001 attgccctga tgggaagtgt gaaaactttc cgtcagggta tttttatttt tccagaaaag
    18061 aaaataattc cgacaggaca ttttatgccc aatacacact tcaaaagtat tccagaaaag
    18121 aaatttccgt aattcgacaa aaatagttga aatgtttccc tggttgtgat agaatctgaa
    18181 agattaaagt atgagatgca tatttcatac aaactcaaaa ccgaacgaaa aagaaaggaa
    18241 aacggtggaa tgatgaaagc ttttctaatc ttagaagacg ggcatgtctt taccggaacc
    18301 agcattggtt cacaaaaaga agtgatcagt gaaatcgtat ttaatacatc catgactgga
    18361 tatctcgaag tgctgactga cccatcctat gcgggacagg cagtatgtat gacgtatcct
    18421 cttattggaa actatggaat ttgttatgat gatcaggagt ccttaagacc ctggccggat
    18481 ggctatattg taagagaatt atcccgtctc cccagcaact tccgttgcca ggacaccatt
    18541 cagaatttcc tgaaaaagtt cgatattccc ggaatcgccg gaatcgatac tcgtgctctc
    18601 accaaaattc ttcgtgaaaa aggtaccatg aacggtatga tcacgaccaa cgaaaattat
    18661 cacctcgatg aaattattcc aaaattaaaa gcgtataata ccggaaaagt tgtagaaaag
    18721 gtaacctgct ctgaaaaatc agttttaaaa ggagaaggct ttaaagttgc acttctggat
    18781 cttggcgcaa agaacaacat cgcacgttcc ttaaatgagc gcggatgcga agtgaccatt
    18841 tatccggcgc ttactacagc agaagagatc ctcgccgcaa atccggacgg aatcatgctg
    18901 tccaacggcc ctggagatcc gaaagagtgc gtatccatta tcaaagagat taaaaaactg
    18961 tacgattcca atgtaccgat ctttgcaatc tgtcttggac atcagctgat ggcactggct
    19021 accggcgcag atacccacaa gatgaaatac ggccaccgcg gcggaaacca cccggtcaag
    19081 gacctggcaa ccggaagagt ttacatttct tcccagaacc acggctacgt cgtagatacc
    19141 gataccctgg atccgaatgt ggcagttccg gcctttgtga acgtcaacga cggcaccaac
    19201 gagggactca aatacgtcgg caaaaacatc tttaccgtac agttccatcc ggaggcatgc
    19261 cctggcccgc aggattccgg atacctgttt gatcgattcc ttgagatgat gggaggtaac
    19321 aaataatgcc aaagaatccg aatattaaaa aagttctggt tatcggttcc ggtccgatcg
    19381 tcatcggaca ggcagcagag ttcgactacg caggaacaca ggcgtgccgt tccctgaaag
    19441 aagagggaat ggaagttgtt cttctgaact ccaacccggc aaccatcatg accgataaag
    19501 atatcgcaga ccgcgtttac atcgagccgc tgaccgtaga agtggtagag cagctcatcc
    19561 agaaggaaaa accggacagc gttcttccga ctctgggagg ccaggcagga ctgaaccttg
    19621 cgatggaact cgaagaaaaa ggcttcttaa aagaaaacaa cgtacgcctg atcggtacca
    19681 cagcacagac catcaaaaaa gcagaggacc gtcaggaatt caaagatacc atggaaaaaa
    19741 tcggcgagcc ggtagcggca tccctggttg tccatgatgt acaggctggt atcgatttca
    19801 ccaacaagat cggttatccg gttgtactgc gtccggctta caccctcggc ggaagcggcg
    19861 gcggtatcgc ttacaacgaa gaagagctga tcgagatcct ctcgaacgga ctccgtcttt
    19921 cccgtgtcgg cgaagtactc gttgaaagat gtatcgccgg atggaaagag atcgagtacg
    19981 aggtaatgcg ggacagcgta ggaaactgca tcaccgtctg caacatggag aacatcgacc
    20041 cggtcggcgt tcacaccgga gacagtattg tcgtagcacc gtcccagacc ctgggagata
    20101 aagagtatca gatgctgcgt acttccgcac tcaacatcat caccgagctc ggcatcaccg
    20161 gcggatgcaa cgtacagtat gcgttgaatc cggacagctt cgagtactgt gttatcgagg
    20221 taaatccgcg tgtcagccgt tcttccgcac tggcttccaa agcgaccggt tatccgattg
    20281 caaaggttgc tgcaaagatc gcacttggct acactctgga tgagatcccg aacgccatta
    20341 ccggaaaaac ctacgcaagc ttcgagccga tgctggatta ctgtgttgta aaaatcccgc
    20401 gtctgccgtt cgataagttt atcagtgcaa aacgtaccct gaccacacag atgaaggcaa
    20461 ccggtgaggt tatgagtatc tgcaacaact tcgagggtgc gctgatgaaa gccatccgtt
    20521 ccctggaaca gcacgtagat tccctgatgt cctatgactt cagccacctg tccaaagacg
    20581 agctgatcga agagcttcac atcgtagacg atatgagaat ctggagaatc gcagaggcga
    20641 tccgtcaggg catctcctac gacgagatcc acgagatcac caaaatcgat gtatggttca
    20701 tcgataagct ggctatcctt gttgagatgg agcaggcatt acaggccaaa gagctggatg
    20761 aggatctgct gcgcgaagca aaacgccttg agttcccgga ttacctggtg gcaaaactgg
    20821 caggaaagac cgaagaagaa gtaaaagcgt taagaaaaca gtatgatatc accgcagctt
    20881 acaagatggt agatacctgt gccgctgagt tcgcagcaac cacaccgtat tactattccg
    20941 tatacggcgg cgaaaacgaa gctgtcgaga caaacgacag aaagaaagtc ctcgtccttg
    21001 gttccggccc gatccgtatc ggacagggta tcgagtttga cttctgctcc gtacattgta
    21061 cctgggcatt caagaaagag ggctatgaga ccatcatcat caacaacaac ccggagaccg
    21121 tcagcaccga cttcgatatc gcagataaac tgtatttcga gccgctgaca ccggaagatg
    21181 tagaaaatat cgtcaacatc gagcatccgg acggagccgt tgtacagttc ggcggccaga
    21241 ctgcgattaa gctgaccgaa gcactcctga aaatgggtgt gccgatcctt ggaacatctg
    21301 cagagaacgt agatgccgca gaggaccgtg agctgttcga tgagatcctg gagcagtgtg
    21361 aaatcccgcg tccgaaggga cacaccgtat tcaccgcaga agaggcgaaa aaagcagcca
    21421 acgagctcgg ctatccggta ctcgtccgcc cgtcctacgt ccttggagga cagggtatgc
    21481 agatcgccat cagcgatgag gacgtcgatg aatttatcgg catcatcaac cggatcgcac
    21541 aggagcatcc gatcctggtt gataaatatc tgcagggcaa ggaaatcgag gttgatgcag
    21601 tctgcgacgg caccgatatt ctgattcctg gtatcatgga gcacatcgag cgcgccggcg
    21661 ttcactccgg agacagtatc tccgtttatc cggcacagag catcagccag catgcaaaag
    21721 acaccatcgt agagtatacg aaacgtctgg caagatccct gcatgtcatc ggtatgatca
    21781 acatccagtt catcgtatgc ggtgaggatg tttacgtcat cgaggtcaac ccgcgttctt
    21841 ctcgtaccgt gccgtacatc agcaaggtaa ccggaatccc gatcgtgccg ctggcaacga
    21901 aagtcatcct cggccacacg atccgcgagc ttggctacga gccgggtctg cagagagagg
    21961 cagattacat tgccatcaag atgccggtat tctccttcga gaagatccgc ggcgcagata
    22021 tcgcccttgg accggagatg aaatccaccg gtgagtgcct tggtattgca aagaccttca
    22081 acgaggctct ttacaaagca t
//
LOCUS       DAOMUF010000014        20576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_425_length_20576_cov_4.490960, whole genome shotgun sequence.
ACCESSION   DAOMUF010000014 DAOMUF010000000
VERSION     DAOMUF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 20576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20576
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_425_length_20576_cov_4.490960"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLN77_01980"
     CDS             complement(<1..560)
                     /locus_tag="PLN77_01980"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HRM43083.1"
                     /translation="MSFFSLDSKFSQIMGRVFDLMMLNIIFLIMCIPIVTIGANFTAM
                     YYVTLKMIKNEETYIFRTYWKSFRENFKQATAIWLILLAVLIVLILDLLLVMRMPGTI
                     TYLRFVFLVLIFFEAMVLSYVFPVLSRFDNTVKNTIKNSILMAIRHLPWTIMILLVNL
                     CPLLIYVFSTTKIVSYLILLMFLLGFS"
     gene            910..2022
                     /gene="ugpC"
                     /locus_tag="PLN77_01985"
     CDS             910..2022
                     /gene="ugpC"
                     /locus_tag="PLN77_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRM43084.1"
                     /translation="MASLSLQHITKRYPNGFEAVKDFNLEIADKEFIIFVGPSGCGKS
                     TTLRMIAGLEEISDGTLKIDDKVVNDVEPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     LRKVPKDQIDKQVKEAAKILDLEKLLDRKPKALSGGQRQRVAMGRAIVRDPKVFLMDE
                     PLSNLDAKLRVQMRTEISKLHERLGATIIYVTHDQTEAMTLGTRIVVMKDGVVQQVDT
                     PQNLYNAPGNLFVAGFIGSPQMNFLDAKVKVNGNDVTLTVGKYNMKLPASKAKAVIDG
                     GYDGKTVVMGIRPENVHDSQMFIETSKDSVVECKINVYELLGAEVYLYFDCEGFPMTA
                     RVDPRTTARSGDTVKFALDMEKVHLFDKETEITITN"
     gene            2219..3310
                     /locus_tag="PLN77_01990"
     CDS             2219..3310
                     /locus_tag="PLN77_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM43085.1"
                     /translation="MVSSQVLQKTLDELRNITRIDLCVTNTDGVLLVTTFPETAIDAE
                     SIRSFVLSAADSQIVQEYHYFKVYDNQNVEYILISKGSSDDAYMIGKVAVCEIQNLII
                     AYKERLDKNNFIQNLLLDNLLLVDVYNRAKKLRIDTDVRRVVYMVETKLEKDISAQET
                     VKSLFASRPRDFITAVDEKSIIIVKELKDTDTFEDMNQTARMLVDMLNAEAMSQVRVS
                     YGNPVSEIKSVSRSYKEAKMALEVGKIFYADKNVVPYNNLGIGRLIYQLPIPLCEMFM
                     TEVFGENLPDTFDEETLTTINKFFENNLNVSETSRQLYVHRNTLVYRLEKLEKSTGLD
                     IRKFDDALTFKIAMMVVSYMTYMNNSENL"
     gene            3430..5718
                     /locus_tag="PLN77_01995"
     CDS             3430..5718
                     /locus_tag="PLN77_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HRM43086.1"
                     /translation="MIELLNTGAYLVNGIDIIPDTEEGRAELKARTGAVPCPADAAKE
                     TIAYGILKDHNVSGNMENLQIKFDKLTSHDITFVGIIQTARASGLEKFPVPYVLTNCH
                     NSLCAVGGTINEDDHMFGLTCAKKYGGVYVPPHQAVIHQFAREMLAGGGKMILGSDSH
                     TRYGALGTMAMGEGGPELVKQLLSKTYDIAMPGVIAIYMTGAPQPGVGPQDIALAIIG
                     AVFANGYVKNKVMEFVGPGVSSLSADYRIGVDVMTTETTCLSSIWRTDEKISEFYQIH
                     GRSADYKELNPGAVAYYDGVVKVELDKIKPMIAMPFHPSNVYTIEELKANLDDILNDV
                     EKKAAISLDGKVPFTLKNKVHDGKLLVDQGIIAGCAGGGFENICDAADILKGHSIGDD
                     AFTLSVYPASTPIYMELARNGKLADLLETGAIVKTAFCGPCFGAGDTPANNAFSIRHS
                     TRNFPNREGSKIQNGQISSVALMDARSIAATAANKGFLTAADEFTGTYTKPAYHFDKK
                     IYENRVFDSKGVADPSVEIQFGPNIKDWPAMPQLAENLILKVVSEIHDPVTTTDELIP
                     SGETSSFRSNPLGLAEFTLSRKDPAYVGRAKEVQVAEKAIQEGNCPAEALPELKPVFA
                     AIHTQYPDIDKTNVGVGSTIFAVKPGDGSAREQAASCQKVLGGWANIANEYATKRYRS
                     NLINWGMLPFLIPEGDLPFTNGDYIFVPEIRKAVEEKADVIKAYVVKDGALVPFEMTL
                     GALTDDEREIIRKGCLINYYRG"
     gene            6613..7278
                     /locus_tag="PLN77_02000"
     CDS             6613..7278
                     /locus_tag="PLN77_02000"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM43087.1"
                     /translation="MEDNDILVVHKHAGMAVQNARMGQMDLEHALLNYLAKQTRAETP
                     GQARTQIPYLAVVHRLDQPVEGVLVFAKNKDAAGKLGRQVKDGTMKKEYLAVCEGKIE
                     KQGVQKFEDFLVKDGRSNTSRVVQPGTAGAKKAVLEYEVIRTQENRTLVQIHLGTGRH
                     HQIRVQFSHAGYPLAGDAKYGAKEKGSLALCAHHLELKHPRTGKRLSFTVKPEGEIFH
                     SFV"
     gene            7438..8658
                     /locus_tag="PLN77_02005"
     CDS             7438..8658
                     /locus_tag="PLN77_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43088.1"
                     /translation="MKRRMIAVLLSGILLCSAAGCGKEAVTDKSTAASSSQEVTETPA
                     AEESPEASSSSADSTAESASASTSSESSGDEGKEADKKRIEITVPHKDGMEALYDTVD
                     GLTVEKGSYIAVVAKDLSAGFWKAVKAGAQQALDDLNASLGYTGNDKVYMTFEGPENE
                     SDTNSQINIIDSVISENPDALILGIIDQQSGQAQMEEASDSQIPVVIADSGIESDLIT
                     STCATDHAAAAKLAADHLCEAIGNSGEVAIVAHQYNTESSAERVEAFKKEIEEKYTDV
                     KVVQVNYGDGEESIADMLKNTLETYPALKGIFCTNEDMADQTLDALADMENTEQIQIV
                     GFDSGEKQQKAIQEGREYGTVTQNPYGLGYAAIVAAVRAIQGLPVDQKIDTGYQWIDK
                     DTIDLETNQKYLYN"
     gene            complement(8736..9827)
                     /locus_tag="PLN77_02010"
     CDS             complement(8736..9827)
                     /locus_tag="PLN77_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HRM43089.1"
                     /translation="MNLNMQAFNINTLLGGLDKVRDVMGVYQQQLLLIVIVFALLNCF
                     FGYALRKLWSVIPGFFLGAAAGYAAGTYTNQTTTITLALAVGTGLLTAIIAFVLYRIG
                     LFLLICGLVGFCLWHLVNPNDITGYGLVALVAFVIGLICVPFERISVILVTSICGALT
                     TIRMAYFFKDLDLNMMFWIVSAILAAAGMLFQFKPWKEADYWEDDDEEEREHRAKARA
                     RRKRRSGFDPLPSRKKKKKRRGSSSSSSGNARDPYRSYQKPSNRTRVSQNTMYDFRFV
                     PEEPDDDDDEKDLPKIIRRRPSAKPAAPEEFDEEETSPKASMGDTKPYTSPSKPQITE
                     DGIPDVDLSAIRQQISEEVQRIYEDDQNS"
     gene            9991..10416
                     /locus_tag="PLN77_02015"
     CDS             9991..10416
                     /locus_tag="PLN77_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43090.1"
                     /translation="MNQDRVYENRRNSYQGNPMERPEPFFAAQPLWGRSMEEQQEARE
                     RQRMQSYYSRTASDLQRLVEAECDRMDYRGSMMYDEFPDRLMMEHTCRNIARQYGREN
                     GGKDEKTAEEELLDLIGVLFYNEMFRRRSRRKHYYNLWL"
     gene            10564..11721
                     /locus_tag="PLN77_02020"
     CDS             10564..11721
                     /locus_tag="PLN77_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRM43091.1"
                     /translation="MELQKLLQDEIREGLYRITISGPRGDSEVSKVRIRPVEIKGKLL
                     FQCQETVGTKEFHKNMTKDDVITRISDWMNGTFKQMQLESDTCTASVLVSKKGTMTIK
                     KKPHMKGTGKPVNLAHNRKKRYILEEGIPVPFLQDLGVMTKEGKIVRTRYDKFRQINR
                     FLEFIEDILPQLPSDREITILDFGCGKSYLTFAMYYYFRELKKLDVNIIGLDLKEDVI
                     AICNGLAEKYGYEKLHFYQGDIASYTGRDEVDMVVTLHACDTATDYALEKAVKWNAKV
                     ILSVPCCQHELNRQIANKELYPIMDYGILKERMAALLTDGIRAKLLENAGYETQILEF
                     IDMEHTPKNLLIRAVKRQEGSKKLPEELKACMEAYHVKPTLADLLTEKEEP"
     gene            11721..14189
                     /locus_tag="PLN77_02025"
     CDS             11721..14189
                     /locus_tag="PLN77_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43092.1"
                     /translation="MNKRAARILILFLVFAASVGVFTHLMSHETTENATDLDAASLPV
                     LYMKTADTTVNRMYGYRQEMNGVTTRENLTPLPTDRSLTLEIDAKGQKIKNVTYTVES
                     TDGGALIENSVLKSFDEDGSYLKADFQLETAILMNQEYTLKLEVAYGNGQSAWYYTRI
                     VQRNGVEVGDYLAYTQMFAQTCLDKTQAEALVPQLEPDETGDNSSFLNVNIHSSLDQI
                     SWGSLAPTIVQQPVQQIKEANETTTSIKQEYMISAQDENGQTEYYTVSEFYRMRESDG
                     EIILLDFERSAQQIFDPELGVLTKSGINLGVTGEDTKYVTNTAGDIVAFVVNGDLWCY
                     NRSANKTIRVFSFRENGSMDDREQHGEHDVNIVRVDDAGDVTFVVYGYMNRGNHEGEV
                     GAAVYYYRAERNVATEEIFVPADISYEMLKKQLDRLSYVNKQREMYLYLNENLCKVDI
                     ETGTTTVVRESIPEDCFVVSESQESIAWMDADNASSAMNITVMNLESGETQRFAVDDG
                     QKIRALGFINEDFVYGMANDSDILKDISGNEVFAMHTVRIVSIDGNVKKEYHQDGYYV
                     TGVSISDGLLELDRVVRQENGYADAPEDHIMNGEQQSQELVTGRLATVDDRREQQFLL
                     EFSTSGKTQSLLTLTPKYIYSTLRTDLTMSYDTGSADLYYVYGKGKLIAILSSPAEAV
                     QLADENVGVVLNSSQSYVWERGNRQQGMRLDETTMPSGFQSASLDENVLKESLGDDYE
                     LLNLTGCTREEILYMISSGYGVVVKTGANESLLLTGYDIFGNSWLYNPATGETTALSD
                     DDSDALFAQNGNVFISYIKKVKIE"
     gene            14266..15657
                     /gene="asnS"
                     /locus_tag="PLN77_02030"
     CDS             14266..15657
                     /gene="asnS"
                     /locus_tag="PLN77_02030"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRM43093.1"
                     /translation="MKLTTIREIYKNREAYLDKEVTVGGWVRSVRDSKTFGFIVLHDG
                     SYFETLQIVYHDEMENFAEISKMNVGAAIIVKGTLVATPQAKQPFEIQAAEVTVEGVS
                     APDYPLQKKRHSMEYLRTISHLRPRTNTFQAVFRVRSLCAYAIHQFFQERDFVYVNTP
                     LITGSDCEGAGEMFQVTTMDLDNIPKTEDGAVDFTQDFFGKKTSLTVSGQLNGETFAQ
                     AFRNIYTFGPTFRAENSNTTRHAAEFWMIEPEIAFADLEDDMELAEAMLKYVIRYVME
                     HAPEEMQFFNSFVDKGLIERLNHVVNSEFGRVTYTEAIELLEKNNDKFDYKVYWGCDL
                     QTEHERYLTEQIFKRPVFVTDYPKEIKAFYMKLNDDNKTVAAMDCLVPGIGEIIGGSQ
                     REDDYEKLLGRMKELGLAEEDYGFYLDLRKYGSTHHAGFGLGFERCVMYLTGMGNIRD
                     VIPFPRTVNNCEL"
     gene            15787..16851
                     /locus_tag="PLN77_02035"
     CDS             15787..16851
                     /locus_tag="PLN77_02035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRM43094.1"
                     /translation="MRFIHIADVHLGMQPDAGFPWSEERGEAIWESFRRIIRLAGREK
                     TDFLLIAGDLFQRQPLLRELKEVNDLFASIPETIVVLIAGNHDYVKRESFYRGFDWAD
                     NVVMLLSPEPECVEVPEKKTAVYGCSYDKKEILENRLDGVRPEGKMKYHLLLAHGGDA
                     RHMPWNPGRMAQAGFDYIACGHIHKPGILIPDKMAYAGALEPTDETQLGPHGYIRGTV
                     DEHGTRIQFVPFARYEYEDLVLNVTEDLTQYALETKLKQELALREDGKIRKILRLKLV
                     GHRAAELEFSPKRLLDCGRVISVEDETRPAYDLEQLKKTYGASLISAYIEVFETKTDA
                     QSQKALDYGLEALLAARRNG"
     gene            16878..18410
                     /locus_tag="PLN77_02040"
     CDS             16878..18410
                     /locus_tag="PLN77_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM43095.1"
                     /translation="MKIEKLEIRNFGKMQNRTLEFSDGIQLISGENESGKSTVHTFIR
                     SMLFGMTRGRGRAAKNDVYSRYEPWENPAYYAGEMVLESGGKRFRLTRNFGRQNANAK
                     LVCLTDGEVLSVEDGDLSMLLGGVSEIVYDNTASIGQLKGRTEEGLADELRNYMANYQ
                     GSSDGELDVAAALDLLKQKRKRLEQEQKKTLAGLEKRKEELETKISVFTEECRQSEEN
                     LQQAKEQMKRDIYQKDVPIERVRKMKKESRKSRKWGAACVALAGFLLFLCILQFFHID
                     SLLTRTGLGLAIAALLYLAYSLFRHRKTEVEQVVTEPEEQAIRRQQWNVDRLKQELGQ
                     KQTVLSNLQSEYEELCISMTEKDHLQEELDALSLAGETIQSLSVQMQSRIGDRLKQQM
                     SKTLSSLTNGRYLQVNMDENLRIGLHTADEYVPLEQVSRGTIEQAYFALRMAAMDVLC
                     GEEELPVILDESFAFYDENRLKETLKWLAENRTQVLLFTCQKREEEALSEMGIPYRKI
                     VL"
     gene            18471..19301
                     /locus_tag="PLN77_02045"
     CDS             18471..19301
                     /locus_tag="PLN77_02045"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRM43096.1"
                     /translation="MLTIGCHLSSSKGYLAMGKEAVSIGANTFQFFTRNPRGSKAKAI
                     DEADVAAFLSYSKEHGIERILAHAPYTLNPCSKDAHTREFAWMTMADDLKRMEYTPGN
                     CYNFHPGSHTGQGAEEGIRLISEMLNEILKPEQTTTVLLETMAGKGTEVGRSFEELAA
                     ILERVELKDHMGVCLDTCHVYDAGYDIVDHLDDVLEGFDRVVGLSKLKAIHLNDSKNP
                     FESHKDRHEKIGEGSLGLAAFERIVTHPALAGLPFYLETPNELDGYAKEIALLRGFAK
                     "
     gene            19321..>20576
                     /gene="rlmD"
                     /locus_tag="PLN77_02050"
     CDS             19321..>20576
                     /gene="rlmD"
                     /locus_tag="PLN77_02050"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRM43097.1"
                     /translation="MENTREKNKRSDGRKKTYFDRENKKKAVRTEKTKSVAPEKGKQK
                     KSSSSSKCPYQKKCGGCTYLNLSYEEQLAKKEQFLKKTLKGIVPVKGMVGMENPYHYR
                     NKVNSAFARKKDGTIISGIYEKGTHKVVPVDECLLEDQRADAIIRDIRSLLKSFKIMI
                     YNEDSGYGLLRHVMVRTGFASGEIMVVLVCVSPVFPSKNNFVKALRKLHPEITTIVLN
                     VNDRTDSMVLGKRDIVLFGRGYIEDELCGCTFRISPQSFYQVNPVQTKNLYEKAISLA
                     GLTGNERVMDAYCGIGTIGLIASRDAREVISVELNPDAVRDAVTNAKRNQIKNVSFYQ
                     NDAGAFMVSMAEKGEKADVVFMDPPRAGSDEAFLSSVVTLSPKKVVYVSCNPETLARD
                     LRYLTKHGYKAEEAWGYDMFAWSEHV"
BASE COUNT         5976 a         4621 c         5646 g         4333 t
ORIGIN      
        1 gaaaagccca gaaggaacat cagaagaatc agatagctga caatctttgt cgtggagaat
       61 acatagatca gcagcggaca gagattgacg agcaggatca tgatggtcca cggcaggtgg
      121 cggattgcca tcaggatgga gttcttgatc gtgtttttta ccgtattgtc aaagcggctc
      181 agtaccggaa atacatagga aagtaccatg gcctcgaaaa agatcagtac caggaaaaca
      241 aaacgcaggt aggtgatggt acctggcatt ctcatgacca gcagaagatc aagaatcaga
      301 acaatcagaa ccgccagcag gatgagccag atggcagttg cctgcttgaa gttttcgcgg
      361 aaggatttcc agtaggtacg gaagatatac gtctcctcat ttttgatcat cttcagcgtg
      421 acgtagtaca ttgctgtgaa attggcgccg attgtcacaa tcgggataca cataataagg
      481 aaaataatgt tcagcatcat caggtcgaag acacggccca taatctgcga gaacttactg
      541 tccaggctga aaaaactcat aatttgttcc tcttttctgt tgaattaagt gtgccgccga
      601 ttaacgttcc ggctggcaga catcatagaa aagtatagcg aatttttcag aaaaatgcaa
      661 tgaagagaat ataaactttt cgtttcgtca ttttgtataa gagtgaacat tttgtttgtg
      721 caaagtggac agaagagaac ggttcgagaa aatccgaaag cagaattgcc aatctgccgg
      781 aggaaagttt gtgtaattgt ggcatggtcg aatgcgggta gaaaaggtat actagtccca
      841 taagcaagag agatgcaaat gcaaaactca taataaaacg catgatgttc atttagggag
      901 gattcaaaaa tggcaagttt atctttacag cacattacaa aaagatatcc gaacggtttt
      961 gaagcagtaa aagacttcaa cctggaaatc gctgacaaag agtttatcat cttcgtagga
     1021 ccatctggat gtggtaaatc tacaacactt cgtatgatcg caggtcttga ggagatctcc
     1081 gatggtactc tgaaaattga cgataaagta gtaaacgatg tagagccgaa agaccgtgat
     1141 atcgcaatgg tattccagaa ctacgctctg tatccgcaca tgaccgtata tgataacatg
     1201 gcattcggtc tgaaactgag aaaagttccg aaagatcaga tcgacaaaca ggttaaagaa
     1261 gcagctaaaa tccttgacct ggagaaactg cttgaccgta aaccgaaagc tctttccggt
     1321 ggtcagagac agcgtgttgc tatgggacgt gccatcgtac gtgatcctaa agtattcctt
     1381 atggacgagc ctctgtcaaa cctggatgca aaacttcgtg tacagatgcg taccgagatt
     1441 tccaaactgc atgagagact gggcgctacc atcatctacg taacacatga ccagaccgag
     1501 gctatgacac ttggtaccag aatcgttgtt atgaaagacg gagttgttca gcaggtagat
     1561 accccgcaga acctgtacaa cgcaccgggc aacctgttcg ttgcaggatt catcggatct
     1621 ccgcagatga acttcctgga tgcaaaagta aaagttaacg gaaacgacgt tactcttact
     1681 gttggtaaat acaacatgaa actgccggct tccaaagcaa aagctgttat cgacggcgga
     1741 tatgacggaa agaccgttgt tatgggtatc cgtcctgaga acgtacatga ttctcagatg
     1801 ttcatcgaaa cttccaaaga cagcgtagtt gagtgcaaga tcaacgtata cgagctgtta
     1861 ggtgcagaag tttacctgta cttcgattgc gaaggcttcc caatgacagc ccgcgtagat
     1921 cctcgtacaa ctgcaagaag cggtgatacc gttaaatttg ctctggatat ggagaaagtt
     1981 catctgttcg acaaagagac agagatcacc attaccaact aatccatatg aacatcagcg
     2041 cgtatgcgca aacatagaga aaaggatcga catccgttcg gatgccggtc ctttttgcat
     2101 atgcaccgcc ctccttttgc gaagcaaaaa atagttgcca atgagaaaaa aataagctat
     2161 agttataaat aagatgcacc ctggagtaaa atacgacagg agaaaggaag tggcagattt
     2221 ggtatcaagt caggtattac agaaaaccct tgatgaatta agaaatatta cgagaattga
     2281 tttatgtgtg acgaatacgg atggtgttct gctggtgacc acattcccgg aaaccgcgat
     2341 tgatgcagaa agcatccgtt cttttgtatt atccgctgcg gacagccaga ttgtacagga
     2401 atatcattat tttaaagtat atgacaacca gaatgtggag tatattttga tttctaaagg
     2461 aagcagcgac gatgcatata tgatcggaaa ggttgccgta tgtgagattc agaatcttat
     2521 cattgcatac aaagagcgtc tggacaaaaa caactttatc cagaaccttc ttctcgacaa
     2581 tcttcttctg gtggatgtct acaaccgcgc gaaaaagctt cgcatcgaca cagatgtgcg
     2641 ccgcgttgtt tacatggtag agacgaagct ggaaaaagac atcagcgcac aggagacagt
     2701 aaagagcctc tttgcttcaa gaccgagaga tttcatcacc gcagtcgatg agaagagcat
     2761 tatcattgta aaagaactga aggataccga caccttcgag gatatgaacc agaccgcaag
     2821 aatgctggtt gatatgctga atgcagaggc aatgtcccag gtacgtgttt cttacggaaa
     2881 tccggtcagc gagattaaga gtgtttcgcg ttcctacaaa gaagcgaaga tggcgcttga
     2941 ggtaggaaaa attttctacg cagacaaaaa cgtggtacca tacaacaacc tcggcatcgg
     3001 ccgtctgatc tatcagctcc cgattccgct gtgcgaaatg tttatgaccg aagtcttcgg
     3061 cgaaaatctg ccggatacgt tcgatgagga aacactgacc acaatcaata aattttttga
     3121 aaataacctg aatgtgtccg agacatcccg tcagctctat gtacacagaa atactctggt
     3181 gtaccgtctg gagaagctcg agaagagcac aggactcgac attcgtaaat ttgacgatgc
     3241 gctgaccttt aaaattgcca tgatggtcgt aagctacatg acgtatatga acaacagcga
     3301 aaatctgtaa aaccgttcag agccgtacag gattgcggga gtgcaaagca cggagcaatc
     3361 cgggtatcga aggggtcaaa agactttttg accctgacat tttatcaaca acaagaggag
     3421 aggaacgaca tgattgaatt actgaacaca ggtgcatacc ttgtcaacgg aatcgatatc
     3481 atcccggata cagaagaagg ccgcgcggaa ctcaaagcgc gtaccggcgc tgtgccgtgt
     3541 ccggcggatg ctgcaaaaga aaccattgcc tatggaatct taaaagacca caatgtttcc
     3601 ggcaacatgg aaaatctgca gatcaaattt gacaaactta catcgcatga cattactttt
     3661 gttggaatca ttcagaccgc gcgcgcgtca ggactggaaa aattcccggt accgtacgtg
     3721 ctgaccaact gccacaactc cctttgcgca gtcggcggaa ccatcaacga agatgaccac
     3781 atgtttggtc ttacctgtgc gaaaaagtac ggcggcgtct atgtaccgcc tcatcaggca
     3841 gtaattcatc agtttgcccg cgagatgctt gccggcggcg gaaaaatgat cctcggatcc
     3901 gattcccaca cccgttacgg tgcactcgga accatggcca tgggcgaggg cggaccggag
     3961 ctggtaaaac agcttctctc caaaacatat gatatcgcta tgccgggagt gatcgcaatt
     4021 tacatgacag gcgcaccgca gccgggcgta ggaccgcagg atattgcact tgccatcatc
     4081 ggtgcagtct ttgcaaatgg ttacgtaaag aacaaagtta tggagttcgt aggacctggc
     4141 gtatcttcct taagcgcaga ttaccggatc ggtgtggacg ttatgacaac cgagacaacg
     4201 tgtctgtcct ccatctggag aaccgatgag aagatcagcg agttctatca gatccacggc
     4261 agaagcgcag attacaaaga gctgaatcct ggcgcggttg cttactatga cggcgttgta
     4321 aaagtagaac tcgacaagat caaaccgatg atcgcgatgc cgttccatcc gagcaatgtt
     4381 tataccattg aagagctgaa agcaaacctc gatgacatcc tgaacgatgt agagaagaaa
     4441 gcagccatca gcctcgacgg aaaagttccg ttcactctga aaaataaagt acacgacgga
     4501 aaactcctgg tagaccaggg aatcatcgca ggatgcgccg gcggcggatt tgagaacatc
     4561 tgcgacgcag ccgatatcct gaaaggccac agcattggcg acgatgcgtt caccttaagt
     4621 gtttacccgg caagtacccc gatctatatg gagctggcaa gaaacggaaa actggcagac
     4681 cttttggaaa ccggtgcgat cgtaaaaacc gcattctgcg gaccgtgctt cggcgcaggc
     4741 gatactccgg caaacaatgc gttctcaatc cgtcactcca ccagaaactt cccgaaccgc
     4801 gagggatcca agatccagaa cggccagatt tcttccgttg ccctgatgga tgcacgttcc
     4861 attgcagcaa ccgcagcaaa caagggattc ctgaccgcag cagacgagtt caccggaaca
     4921 tacacgaaac cggcatacca cttcgacaag aagatttacg agaaccgtgt cttcgacagc
     4981 aagggtgtgg cagatccgtc tgtagaaatc cagttcggac cgaacatcaa agactggccg
     5041 gctatgccgc agcttgccga gaacctgatt ctgaaggttg tttccgagat ccacgatccg
     5101 gttacgacaa ccgacgagct gattccatcc ggcgagactt cttccttccg ttccaacccg
     5161 ctcggactgg cagagttcac cctgtcaaga aaagatccgg cttacgtggg acgtgcaaaa
     5221 gaagtacagg tcgcagagaa agccatccag gaaggaaact gcccggcaga ggcacttccg
     5281 gaactgaaac cggtcttcgc agccatccat acccagtacc cggatatcga caagacaaac
     5341 gtcggtgtcg gatccaccat cttcgctgta aaaccgggcg acggctctgc gcgtgagcag
     5401 gcagcttcct gccagaaggt attaggcggc tgggcaaaca tcgccaacga gtatgcaacg
     5461 aagcgctacc gctccaacct gatcaactgg ggcatgcttc cattcctgat cccggaggga
     5521 gatctgccgt ttacaaacgg agattacatc tttgtaccgg agatccgcaa ggcagtggaa
     5581 gagaaagccg atgtgatcaa agcgtacgtt gtaaaagatg gagcacttgt tccgttcgag
     5641 atgacgttag gcgcgctgac cgatgatgag agagaaatca tccgcaaggg atgcctgatc
     5701 aactattata gaggataagt atttgagaaa gagctgtgtg gcaggagaaa agtctgtttc
     5761 atagctcttt ttctgcacac ttatttgaaa caaaatatca gaaaacaata taaaattcag
     5821 aaaataaaca caataattaa gaaaaggaaa agcgatataa aaaaacagta aaaatatttt
     5881 aaaaatgtgt tgacaaatgc tgacggatct gttatattaa aatcacaaag aaacaagaaa
     5941 acaaaaacct aaccaagagg tacaaagaga aagacttggg atcccctttt caagagaaca
     6001 aaccattttc atagaaagag acgagaaatc ttctatagaa gaatggaaaa aagggaaaag
     6061 gaagatgttc ttaagatgag agacttccga aaggaaagaa aacctgagat aaagatccat
     6121 acaaaagaag ttcgaaagaa aaccttttga taaacatgag atcagagcag atcttaggaa
     6181 atcctaagga aaagattttc ggagtggaaa tcttaaccgc aaaggaaaag cctctgaaaa
     6241 agaacatctt cccgaaaaaa agaaaatcta caacagggaa atggaagaga aacgatcttt
     6301 gaaatatcaa acgaaagcga aaacaaagat cacagatcgg taaagaaaga aaaccagtga
     6361 aaatccttga aagtagagaa aaaactgaat atggaaaata aagaaaaagc ggtcgctgta
     6421 tcagaaaaaa tgattggtat ggcgatcgct ttttgtgttg tgtaattcgt ccgccgggca
     6481 tgtttttctt gacagatgcg gcggaaatgg tgcatacttc ttaaagcata acagcggaga
     6541 aatccgtgta tcgaaaataa gaagtcagaa caggagaaga aagtggctgg attttgcaga
     6601 gaagatattt taatggaaga taacgatatt cttgttgttc acaagcacgc gggtatggca
     6661 gtgcagaatg cgcggatggg acagatggat ctggagcatg cgctgctgaa ttatttggcg
     6721 aagcagacaa gggcggaaac accgggtcag gcgcggactc agattccata tctggcggtc
     6781 gttcaccggc tcgatcagcc ggttgagggc gtgctggtgt ttgcgaaaaa caaagacgcc
     6841 gcaggaaaac tgggacggca ggtgaaggac gggacgatga agaaagagta tctcgccgtc
     6901 tgtgagggaa aaatcgaaaa acagggcgtg cagaagtttg aggatttcct tgtaaaagac
     6961 ggacggagca acacatcccg cgtcgtacag ccaggcaccg ccggagcgaa aaaagcggtg
     7021 ctggaatatg aagtgataag gacacaggag aaccgaaccc tggtgcagat ccacctcgga
     7081 acgggccgcc accaccagat ccgcgtccag ttttcccatg cgggatatcc gctggcgggg
     7141 gatgcaaaat atggggcgaa agagaaagga tctctggcgc tgtgcgcgca tcatctcgag
     7201 ctgaagcatc cgcggacagg caaacggctt tcgtttaccg taaaaccaga aggtgaaatc
     7261 ttccacagct ttgtgtaact attcagagct atgtgaaatt gcaaaaaagt tcagaaaatc
     7321 ataagaaaac aggactggac tatttttacg gtttgtgtat aatagagaaa ggttatgtaa
     7381 taaaactgta aaaagtaatg gaaaatgatt catcaggcaa agcagaaagg gagaattatg
     7441 aaacgcagaa tgattgcagt actgctttcc ggaatcctgc tgtgctcggc agctggatgc
     7501 ggaaaagaag cagtgacaga caagagcacc gcagcatcgt cgtctcagga agtgacagaa
     7561 actccggcgg cagaagaaag cccggaggcc tcatcgtcca gcgcagattc tacagcagag
     7621 tcagccagtg catccacatc ttctgaaagc tctggagatg aaggaaaaga agcagacaaa
     7681 aaacgaatag aaatcaccgt tccgcacaaa gacggcatgg aagcattgta cgacaccgtg
     7741 gatggactta ccgttgaaaa aggctcttac atcgccgtgg ttgcaaaaga cttaagtgcc
     7801 ggtttttgga aagccgtaaa agccggtgcg cagcaggcac ttgacgatct gaacgcctcg
     7861 cttggctaca ccggaaacga caaagtttat atgacatttg aaggcccgga aaatgaaagt
     7921 gacaccaact ctcagatcaa tatcatcgac tctgtcattt cggaaaatcc ggacgcgctg
     7981 atccttggca tcattgacca gcagtcggga caggcacaga tggaagaggc ttccgacagc
     8041 cagattccag tcgtcatcgc ggattccggt atcgagagtg atctgatcac ctcgacatgt
     8101 gcgactgatc acgcagcggc agcaaagctt gcggcagatc atctctgcga ggccatcggc
     8161 aacagcggag aggttgcgat tgttgcacat cagtacaaca ccgagagcag cgcagagcgc
     8221 gtggaagcat ttaaaaaaga gatcgaagaa aaatataccg acgtgaaagt ggttcaggtg
     8281 aattacggag atggagaaga atccatcgcg gacatgctga aaaatacact tgaaacctac
     8341 ccggctctga agggcatctt ttgcacgaac gaagacatgg cagaccagac gctggatgcg
     8401 ctggcggata tggaaaatac tgagcagatt cagattgtcg gttttgattc cggtgaaaaa
     8461 cagcagaaag cgatccagga gggacgcgag tacggtaccg taacgcagaa tccgtacggt
     8521 cttggctatg cggcaatcgt ggcggccgtc cgcgcgattc agggacttcc ggtagatcag
     8581 aagatcgata cgggatatca gtggatcgac aaagacacga tcgatctgga gacaaatcag
     8641 aagtatcttt ataactgaaa atcaggataa aaaatgaacc cggcagacaa gggaatgttc
     8701 ctttgcctgc cgggttttct gcgctgcagt gcggattacg agttctgatc gtcttcgtaa
     8761 attctctgta cctcttcgga aatctgctgc cggatcgcgg agagatcgac atccgggatg
     8821 ccgtcctcgg tgatctgcgg tttggaagga gaggtatacg gcttcgtgtc gcccatggaa
     8881 gcttttgggg atgtttcttc ttcgtcaaac tcctccggag cggccggttt tgcggatggc
     8941 cggcggcgga tgatttttgg aagatctttc tcatcgtcgt catcgtcagg ttcttccgga
     9001 acaaaacgga aatcgtacat ggtgttctgc gaaacacggg tacggttcga cggtttctga
     9061 tagctgcggt acggatctct tgcgttgccg gaagaagagg aagaggagcc gcggcgtttc
     9121 tttttcttct ttctggatgg aagaggatcg aaaccggatc tgcgcttccg tctggcgcgc
     9181 gcttttgcac ggtgttcgcg ttcttcttcg tcgtcatcct cccagtagtc cgcctctttc
     9241 cacggtttaa actggaagag catgcctgct gcggcgagaa tggcagagac aatccagaac
     9301 atcatgttca gatcgaggtc cttgaagaaa tatgccatgc ggatcgtcgt gagggcgccg
     9361 cagatggagg tgaccaggat tacggaaatg cgttcgaacg gaacgcagat caggccgatc
     9421 acgaaagcga cgagggcgac gagtccataa cctgtgatat cgtttggatt gacgagatgc
     9481 cagaggcaaa agccgacgag cccgcagatc agaagaaaaa gtccgatccg gtagaggacg
     9541 aaggcaatga ttgcggtcag aagaccggtg ccgacggcaa gcgccagggt gatcgtcgtg
     9601 gtctgattcg tgtaggtacc cgctgcataa ccggcggccg cgccgagaaa aaatccggga
     9661 atgacgctcc agagcttgcg gagcgcatag ccgaagaaac agttcagcag ggcaaaaaca
     9721 atcacaatca gaagaagctg ctgctggtaa acacccatga cgtcccggac tttatcgaga
     9781 ccgccaagca gcgtgttgat gttgaaagct tgcatattca gattcatgat attcactcct
     9841 atatgatgta tcggaaaaat agatacaggt tgtattttag tacggaacgg cggttttggc
     9901 aagtgaaatt gtgtttgaga tctgggaaac ggcagaatgg cggcggtgtg catatggtat
     9961 aaggaagagc aaaaaggaaa taaaacaggc atgaatcagg atagagtgta tgaaaataga
    10021 agaaattcct atcagggaaa tccgatggag cggccggagc cgttttttgc ggcacagccc
    10081 ttatggggaa gaagtatgga ggagcagcag gaagcgcggg aacggcagcg gatgcagtcg
    10141 tattattccc ggacggcatc tgatctgcag cggctggtgg aggcagagtg tgaccgcatg
    10201 gattaccgcg gcagcatgat gtacgacgaa ttcccggacc ggctcatgat ggagcacacc
    10261 tgccggaata tcgcacgtca gtacggcagg gaaaacggcg ggaaggatga gaaaacggca
    10321 gaagaggaac tgctcgattt gatcggcgtt cttttttata atgaaatgtt ccggaggcgg
    10381 agcaggagga aacattatta caatctgtgg ctttaaaccg cgccataaag taataagagg
    10441 gtaacacatt tttaataatt tttctgttgt ggttttgcgg aaaacagatt acaatattag
    10501 cggggttgta aggggcttta ttacacatgt ttacaaacga ttcaggaagt ttggagagta
    10561 taaatggaat tacagaaatt attacaggat gaaatccggg aagggctgta ccgtatcacg
    10621 atcagcggcc caagagggga cagtgaagtg tccaaagtga gaatacgccc ggttgaaata
    10681 aaaggaaaac tgctctttca gtgccaggag accgtcggca cgaaggagtt tcataaaaat
    10741 atgacaaaag acgacgtgat cacccggatc agcgactgga tgaacggaac cttcaagcag
    10801 atgcagctcg aatccgacac ctgcacagca tccgttctgg tcagcaaaaa gggaacgatg
    10861 acgatcaaaa agaaaccgca tatgaaagga accggcaaac ccgtcaatct ggcgcacaac
    10921 cggaagaaac ggtatattct ggaagaagga atcccggttc cttttttaca ggatcttggt
    10981 gttatgacaa aagaaggcaa aatcgtgcgg acccgctacg acaagttccg ccagatcaac
    11041 cgctttctgg agttcatcga agatatcctg ccgcagctcc catcggaccg cgagatcacg
    11101 attctcgatt tcggctgcgg aaaatcgtat ctgacgtttg cgatgtatta ttacttccgc
    11161 gaactgaaaa aactggatgt caatatcatc ggacttgact tgaaagagga tgtcatcgca
    11221 atctgcaacg gactggcaga gaaatatggc tatgaaaagc tgcatttcta tcagggtgac
    11281 atcgcttctt ataccggccg cgacgaggtc gatatggtgg tgacgctgca cgcgtgcgac
    11341 acggcgactg actatgcgct ggaaaaggcc gtcaaatgga atgcaaaagt tattctttcc
    11401 gttccgtgct gccagcatga gctgaaccgc cagattgcaa acaaagaact gtatccgatc
    11461 atggattacg gcatcttaaa agagcgcatg gcggcgcttc tgaccgacgg aatccgggca
    11521 aagcttctgg agaatgcagg ttatgagaca cagattctcg aattcatcga catggagcac
    11581 acgccgaaga accttctgat ccgcgcggta aaacgccagg aaggctcgaa aaaactgccg
    11641 gaagaactga aagcgtgcat ggaagcgtac catgtgaagc cgacactggc agacctcctc
    11701 acagaaaagg aggagccgtg atgaataaaa gagcagcaag gattctgatc ctttttcttg
    11761 tttttgccgc atcagtcggt gtgttcacac acctgatgag ccacgagacg acggagaatg
    11821 cgaccgatct ggacgcagcg agtcttccgg tgctgtacat gaagaccgcg gacacgacag
    11881 tgaaccgcat gtacggatac cgccaggaga tgaacggcgt aacgacaaga gaaaacctga
    11941 caccgctgcc gacggaccgt tcgcttacgc ttgaaattga tgcgaaggga cagaaaatta
    12001 aaaacgtgac ttatacggtg gaaagcacag acggcggagc gctgattgaa aactcggtgc
    12061 tgaaatcgtt tgacgaggac ggaagctatc tgaaagcaga cttccagtta gagaccgcga
    12121 ttctgatgaa tcaggaatat acgctgaagc tcgaggttgc ctacggaaac ggccagtctg
    12181 cctggtatta cacccgcatt gtacagcgca acggcgtgga ggtcggcgat tatctggcct
    12241 acacgcagat gtttgcgcag acatgcctgg ataaaacgca ggcggaagcg ctcgttccgc
    12301 agcttgagcc ggatgagacg ggggataatt cttcgttcct caatgtcaac attcattcca
    12361 gcctcgacca gatttcctgg ggaagcctcg caccgacgat cgtacagcag ccggtgcagc
    12421 agatcaaaga agcgaatgag acgacgacca gcatcaaaca ggagtacatg atcagtgcgc
    12481 aggatgagaa cggacagacg gagtattata ccgtaagcga gttctaccgg atgcgagagt
    12541 ccgacggaga gattattctg ctggacttcg agcggagcgc acagcagatc tttgatccgg
    12601 agcttggtgt tctgacgaaa tccggcatta acctcggtgt gaccggcgag gataccaagt
    12661 atgtgacgaa tacggcaggt gatattgtcg catttgtagt aaacggtgat ctctggtgct
    12721 acaaccgcag cgccaacaag acgatccgcg ttttcagctt ccgtgagaac ggcagcatgg
    12781 atgaccggga acagcacgga gagcatgatg tcaacattgt acgtgtggat gatgcgggcg
    12841 atgtgacgtt tgttgtttat ggctatatga accgcggaaa ccacgagggt gaagtgggag
    12901 cagccgttta ctattatcgc gccgagcgga atgtggcaac cgaggagatt ttcgttccgg
    12961 cggatatttc ctacgagatg ctgaaaaaac agctcgaccg cctctcctac gtcaacaagc
    13021 agagagagat gtatctctat ctgaatgaga acctgtgtaa ggtagatatt gaaaccggca
    13081 cgacgacggt ggtccgggaa agtatcccgg aggactgctt tgtagtatcg gaaagccagg
    13141 agagcatcgc gtggatggat gcggacaatg caagcagtgc gatgaacatc accgtgatga
    13201 atctggagag cggggaaacg cagcgttttg cggtggatga cggacagaag atccgggcac
    13261 ttggctttat caatgaagac tttgtctatg gtatggcaaa cgacagcgat attttaaaag
    13321 atatttccgg caatgaagtt ttcgctatgc atacggtgcg gattgtcagc atcgacggaa
    13381 acgtgaaaaa agagtatcac caggacggct attatgtgac aggcgtgtcg atttcggacg
    13441 gccttctgga gctggaccgc gtggtacgcc aggaaaacgg ctatgcggat gcgccggagg
    13501 atcacatcat gaacggcgag cagcagagcc aggaactggt gacgggccgc ctggcaacgg
    13561 tggacgacag aagagagcag cagttccttc tggaattctc gacgagcggc aaaacacagt
    13621 cgcttctgac actgacaccg aaatatatct attcaacctt acggaccgat cttacgatgt
    13681 cgtacgacac gggcagcgca gatctttact atgtatatgg aaaaggaaaa ctgatcgcta
    13741 ttttaagctc cccggccgag gccgtacagc ttgccgatga gaatgtcggc gttgtgctga
    13801 acagcagcca gtcctatgtg tgggagcgcg gaaaccgcca gcagggcatg cgcctcgacg
    13861 agacaacgat gccgtcggga ttccagagtg catctctgga cgaaaacgtg ctgaaggagt
    13921 cactcggtga tgattacgaa ctgctgaacc tgacgggctg cacaagagaa gaaattctct
    13981 acatgatcag cagcggctac ggcgttgttg taaagaccgg cgcgaacgag agtctgcttc
    14041 tgaccggata cgatattttt ggaaattcct ggctgtataa cccggcaacg ggcgagacaa
    14101 ctgcactttc tgacgatgac agcgatgcat tgttcgcaca gaacggcaat gtcttcatca
    14161 gttatattaa aaaagtaaag atcgagtgat ttgcggggtt ttctaatgac gcaaaaagtg
    14221 ctagaataaa gaaagcacta tgaatattta gggagaaaaa agacaatgaa acttacaacc
    14281 atccgagaga tttataaaaa ccgggaagca tacctggaca aagaagtaac cgtaggcggc
    14341 tgggtgcgca gcgtacgtga ttccaaaacc tttggtttca tcgttctgca cgacggaagc
    14401 tatttcgaaa ccctgcagat cgtataccac gatgagatgg aaaacttcgc tgagatttcc
    14461 aaaatgaacg tcggcgcagc catcatcgta aaaggtaccc tcgttgctac cccgcaggca
    14521 aaacagccgt ttgagattca ggcggcagaa gttaccgtag agggcgtttc cgcaccggat
    14581 tacccgctgc agaaaaaacg ccactccatg gaataccttc gtaccatttc ccatctgcgt
    14641 ccgagaacca acaccttcca ggcagtattc cgtgttcgtt ccctctgcgc ctatgcgatc
    14701 catcagttct tccaggagag agacttcgtt tatgtcaaca ccccgctgat caccggaagc
    14761 gactgcgagg gagccggcga gatgttccag gtaaccacca tggatcttga caatattccg
    14821 aaaaccgagg acggcgctgt tgatttcaca caggacttct tcggaaagaa gacaagcctt
    14881 accgtaagcg gacagttaaa cggcgagacc tttgcacagg cattccgcaa tatctacacc
    14941 ttcggaccga ccttccgtgc agagaattcc aacaccaccc gccatgcagc agagttctgg
    15001 atgatcgagc cggaaatcgc tttcgctgat ctggaggacg atatggagct tgctgaggca
    15061 atgctgaaat atgtcatccg ctacgttatg gagcatgcac cggaagaaat gcagttcttc
    15121 aacagtttcg tggacaaggg actgatcgag cgtctgaacc atgtcgtaaa ttctgagttc
    15181 ggccgtgtaa cctacaccga ggctatcgag cttctggaga aaaacaacga caaattcgat
    15241 tacaaagtat actggggatg cgatctgcag accgagcatg agcgttatct gaccgagcag
    15301 atcttcaaga gaccggtttt cgttaccgat tatccgaagg agatcaaagc gttctacatg
    15361 aagctgaacg atgacaacaa gaccgttgcc gctatggact gtctggttcc tggcatcgga
    15421 gagatcatcg gcggaagcca gcgtgaggac gactacgaga aactgcttgg ccgtatgaaa
    15481 gagctgggac ttgctgagga agattacgga ttctatctgg acctcagaaa atatggttct
    15541 acccaccatg caggattcgg actgggattt gaaagatgcg tcatgtatct gaccggaatg
    15601 ggcaatatcc gcgacgtcat cccgttcccg agaacagtaa acaactgcga gctgtaatta
    15661 cgaaaagtga tatagaaact taaaaaatac ggatgccggg tgcagtggaa tccaatgtgg
    15721 atttggtgca cctgacgtcc gtattgtagt aatagagaaa atgaatccag aaaggggtat
    15781 gcgcctatgc ggtttattca tattgcggac gtgcatctcg gcatgcagcc ggatgcggga
    15841 tttccctgga gcgaggagcg cggggaggcc atctgggaga gcttccggcg gatcatccgc
    15901 ctggccggtc gggaaaagac ggactttctg ctgattgccg gggatctgtt tcagcgtcag
    15961 ccgcttctgc gggaactgaa agaggttaac gatctttttg cgtccatccc ggagacgatc
    16021 gtcgtgctga ttgccggaaa ccacgattat gtgaagcggg agagctttta ccgcggtttc
    16081 gactgggcag acaacgtggt aatgctcctg tcgccggagc cggagtgtgt tgaagttccg
    16141 gagaagaaga cggcggtgta cggatgcagt tacgacaaaa aagagatcct ggaaaaccgg
    16201 ctggacggcg tgcggccgga gggaaaaatg aaatatcatc tgctgctcgc gcacggcggc
    16261 gatgcccgcc acatgccgtg gaatccgggc cgcatggcgc aggctgggtt tgattatatt
    16321 gcgtgcgggc acattcataa gccggggatt cttattcccg acaaaatggc atacgcaggc
    16381 gcactcgaac cgacggatga gacgcagttg gggccgcacg gctacatccg cggaacggtg
    16441 gacgagcacg gcacgcggat tcagtttgtg ccgtttgcca gatatgagta cgaagatctg
    16501 gtgttgaacg tgacggagga tctgacacag tatgcgctgg agacgaagct gaaacaggag
    16561 ctggcgctgc gggaggatgg gaagatccga aaaatcctcc gcctgaaact tgtgggacac
    16621 cgtgccgccg agctggaatt ttctccgaaa cggctgctgg actgcggcag agtcatctcc
    16681 gtggaggatg aaacacgtcc ggcgtacgat ctggaacagc tgaagaaaac gtatggcgct
    16741 agtctgatca gcgcctatat tgaagtattt gaaacaaaga cggatgcgca gtcacagaag
    16801 gcgctcgact atggtctcga ggcgctgctg gcggcacgca ggaacggctg aaaaaaagat
    16861 tgcgggaggc gtagaagatg aaaatagaaa aactggaaat ccgcaatttt ggaaaaatgc
    16921 agaaccgcac actggaattt tccgatggga ttcagctcat cagcggggaa aatgagagcg
    16981 gaaaatcgac cgtccatacc ttcatccgca gcatgctgtt cggcatgaca cgcggccggg
    17041 ggcgggcggc aaaaaatgat gtctacagtc gttatgagcc atgggaaaat ccggcatact
    17101 atgcgggaga gatggtgctt gaaagcggcg gcaaacggtt ccgcctgaca cggaatttcg
    17161 ggcggcagaa cgccaatgca aagctggtgt gcctgacgga cggcgaggtg ctctccgtgg
    17221 aggacggcga tctttccatg ctgctcggcg gtgtcagtga gattgtgtat gataatacgg
    17281 cttcgatcgg gcagttaaaa ggcagaaccg aggaagggct tgcggatgag ctgcggaact
    17341 atatggcaaa ttaccagggg agcagcgatg gagagctcga tgtggcggcg gcgctggatc
    17401 tgctgaagca gaagagaaag cgcctggagc aggaacagaa aaagacgctg gcgggactgg
    17461 aaaaacggaa ggaagaactt gagacgaaaa tttccgtttt tacggaggag tgccgtcaga
    17521 gcgaggagaa tctgcagcag gcgaaagagc agatgaagcg ggatatttat cagaaggatg
    17581 tgccgatcga acgggtgcgg aagatgaaaa aagaatctcg gaagagccgc aaatggggtg
    17641 ccgcgtgcgt ggcgctggct ggatttctct tgttcttatg cattctgcag ttcttccata
    17701 ttgattcgct gctgacgaga acgggactcg ggcttgcgat tgcggcgctc ttgtatctgg
    17761 cgtacagtct gttccgtcac cggaaaacgg aggtcgagca ggtcgtgacg gagccggagg
    17821 agcaggcgat ccgccgccag cagtggaatg tggaccgcct gaagcaggag ctggggcaga
    17881 aacagacggt gctgtcgaac ttgcagagtg aatacgaaga gctctgcatt tccatgacgg
    17941 aaaaagatca tctgcaggaa gaactcgatg cgctttccct ggcgggggag acgatccaga
    18001 gtctctccgt acagatgcag agccggatcg gcgaccgctt aaaacagcag atgtcaaaaa
    18061 cgctgtcatc gctgacgaat ggccgctatc tgcaggtgaa tatggatgag aatctgcgga
    18121 tcggccttca caccgcagac gaatatgtgc cgcttgaaca ggtcagccgc ggcacgatcg
    18181 agcaggcgta ctttgcgctc cgcatggcgg cgatggatgt gctctgcggc gaggaggagc
    18241 tgccggtgat tctcgatgaa tcgtttgcgt tttacgatga aaaccggctg aaagaaactt
    18301 tgaaatggct ggcggaaaac cggacgcagg tgctgctctt tacgtgtcag aagcgggagg
    18361 aggaagcact ctcggagatg ggaattccgt accggaaaat tgtgctttaa gtaatgcggc
    18421 tcgcagagaa tcggtgagtt gtggtttgga aataaaggag aatagaagtt atgctgacaa
    18481 taggatgtca tttatcatcg tccaaaggat atcttgccat gggaaaagag gcggtgtcca
    18541 tcggggcgaa tacctttcag ttttttacaa gaaacccgag aggaagcaag gcgaaagcca
    18601 tcgacgaggc ggatgtcgca gcgtttcttt cttacagtaa ggagcatgga atcgagcgca
    18661 ttttggcaca tgcgccgtac acgctgaatc cgtgctcgaa ggatgcgcac acgagagagt
    18721 ttgcatggat gacgatggcg gatgatctga aacggatgga gtatacgccg ggaaactgct
    18781 acaatttcca cccgggcagc cataccggac agggggcgga agagggaatc cgtctgattt
    18841 cggagatgct gaatgaaatt ttaaaaccgg agcagacgac cacggtgctg ctcgagacga
    18901 tggcaggaaa aggaaccgaa gtcggccgct cgtttgagga gctggcggcg attctggagc
    18961 gcgtggaatt aaaagaccac atgggtgtct gcctggatac gtgccatgtg tacgatgcgg
    19021 gatacgatat tgtggatcac ctcgatgatg tgcttgaggg gttcgaccgc gtggtaggac
    19081 tttcgaagct gaaagcgatt catctgaacg acagcaagaa tccgtttgag agccataagg
    19141 accgccatga gaagatcggc gagggatcgc tggggcttgc ggcatttgag cggattgtga
    19201 cacatccggc gctggcggga ctgccgttct atctggaaac accgaatgag ctggacggat
    19261 atgcaaaaga gatcgcgctg ctgcgcgggt ttgcgaaata gaggaagaag gtaagagaaa
    19321 gtggaaaaca cgagagagaa gaataaaaga tctgacggaa gaaaaaagac gtatttcgac
    19381 agggaaaata aaaagaaagc cgttcgtaca gagaaaacaa agagcgtggc accggaaaaa
    19441 ggaaagcaga aaaagagttc atcatcatcg aaatgtcctt atcagaaaaa atgcggcgga
    19501 tgcacatatc tgaacctctc ctacgaagag cagctcgcga aaaaagagca gttcctgaag
    19561 aagactctga agggcatcgt cccggtcaaa ggcatggttg gcatggaaaa tccttatcat
    19621 taccggaata aagtcaattc cgcctttgca cgcaaaaaag acggaacgat catatccggc
    19681 atttatgaaa agggcacaca caaagtggtc ccggtcgatg agtgcctgct cgaggatcag
    19741 cgtgccgatg ccatcatccg cgatatccgc agtctgctga agagcttcaa aatcatgatc
    19801 tacaacgagg acagcggcta cggcctcctg cgccacgtca tggtgcgcac cggctttgct
    19861 tccggcgaga tcatggtcgt cctcgtctgc gtatcaccag tcttcccgtc aaaaaacaac
    19921 ttcgtaaagg ccctgcgcaa actccacccg gagatcacca cgatcgtact gaacgtcaac
    19981 gaccgcaccg acagcatggt actcggaaaa cgcgacattg tcctcttcgg acgcggttac
    20041 atcgaggatg aactctgcgg ctgcaccttc cgcatctccc cgcagtcctt ctaccaggtc
    20101 aacccggttc agacgaaaaa cctgtacgaa aaagccatct ccctggccgg cctcaccgga
    20161 aacgaacgcg tcatggacgc ctactgcgga atcggaacca tcggcctcat cgcatcccgc
    20221 gatgccagag aagtcatcag cgtcgaactg aacccggacg ccgtccgcga tgccgtaaca
    20281 aacgccaaac gaaaccaaat caaaaacgtc tccttctacc agaacgacgc cggcgccttc
    20341 atggtctcca tggccgaaaa aggcgaaaaa gccgacgtcg tcttcatgga cccgccaaga
    20401 gccggcagtg atgaagcctt tctgtcctct gtcgtaaccc tttcaccgaa aaaagtggtc
    20461 tacgtctcct gcaatccgga gacattggcg agagacctgc ggtatctgac gaaacacgga
    20521 tataaggcag aagaggcatg ggggtatgat atgttcgcgt ggtcggagca tgtgga
//
LOCUS       DAOMUF010000015        19656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_466_length_19656_cov_3.467017, whole genome shotgun sequence.
ACCESSION   DAOMUF010000015 DAOMUF010000000
VERSION     DAOMUF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 19656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19656
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_466_length_19656_cov_3.467017"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..913)
                     /locus_tag="PLN77_02055"
     CDS             complement(68..913)
                     /locus_tag="PLN77_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRM43098.1"
                     /translation="MGMIRAAGLGYEYLRYEEEGQEPEVTKAIEDVSLEIQKGQFIAV
                     LGHNGSGKSTLAKHINALLVPTEGTMWVDDMKTTDEEDVWKIRQKAGMVFQNPDNQII
                     GNVVEEDVGFGPENLGVPTDEIWKRVEESLTATGMISYRAHSPNKLSGGQKQRVAIAG
                     VMAMQPECIVLDEPTAMLDPNGRKEVLRAVRKLNEEKGVTVILITHYMEEVVFADRVF
                     VMDNGKLVMQGTPREIFSEVEKLKELRLDVPQVTLLAYELRKNGVDLPEGILTIEELV
                     NALCH"
     gene            complement(918..1523)
                     /gene="yihA"
                     /locus_tag="PLN77_02060"
     CDS             complement(918..1523)
                     /gene="yihA"
                     /locus_tag="PLN77_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRM43099.1"
                     /translation="MVIKQVELQHVCGITSKLPDTGQVEVAFAGKSNVGKSSLINALM
                     NRKSLARTSAQPGKTQTINYYHINEEMYLVDLPGYGFARASEEVKAKWGKLIEDYLHQ
                     SKMLHAVFLLIDIRHAPSNNDRIMYDWILRNGYQPIIIATKLDKIKRSQLQKQIKLIR
                     ETLEVEKDTIVIPFSAETKQGREEIWNLIDEIRSDAEESGE"
     gene            complement(1558..>2495)
                     /locus_tag="PLN77_02065"
     CDS             complement(1558..>2495)
                     /locus_tag="PLN77_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRM43100.1"
                     /translation="EVLFIATANDIQNIPRPLLDRMEVIEISSYTENEKFHIAKEHLV
                     PKQKKANGLKEAQLVIEDEALMEVISGYTREAGVRSLERQIGRICRKSARKILEEQVK
                     EVVVTKENLEEFLGKERYEFQPANEKPEIGIVRGLAWTAVGGDTLQIEVNVMPGKGEF
                     LLTGQLGDVMKESAQAGISFIRSVAEQYEIPKEFFEKHDIHIHIPEGAVPKDGPSAGI
                     TMATAMVSAITKVPVKADVAMTGEITLRGRVLPIGGLKEKLLAAKKAGIHMVLVPEKN
                     EPDVRELDAEITDGLQIEFVGSMEQVLKLALVEKK"
     assembly_gap    2496..2510
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2511..3900)
                     /locus_tag="PLN77_02070"
     CDS             complement(<2511..3900)
                     /locus_tag="PLN77_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRM43101.1"
                     /translation="MEEVTKVLPAIALRGTTILPDMIVHFDISRPKSVRAVEEAMLGD
                     QKIFLITQKDPDTEVPGLLDVYKIGTIAVIKQVVRMPKDVLRILVEAERRAELLNFEE
                     EESEYLRAEVALFTGSDEMQFSDIEQEAMLRNLKELFAVYCEQTQKVSKELAGQILGT
                     EEVGKLVDQICMNLPLDYVNRQKLLEAVELSERYDTLCVMIANEIQIQEIRSEIQGKI
                     KERIDKNQRDYILREQLKVIREELGEDNSRTEIENYKKQLEKLKASKEVKERISEEIH
                     RLENSASNPSEAGVIRGYIETLLGLPWDKASRDNQDLARAREILEEDHYGLSKVKERI
                     LEFLAVRTLTKKGESPILCLAGPPGTGKTSIARSVARALNKKYVRICLGGVRDEAEIR
                     GHRRTYIGAMPGRIAAGLHQAKVKNPLMLLDEIDKVSADYKGDTSSALLEVLDSEQNN
                     KFADHYVELPIDL"
     gene            complement(3922..5217)
                     /gene="clpX"
                     /locus_tag="PLN77_02075"
     CDS             complement(3922..5217)
                     /gene="clpX"
                     /locus_tag="PLN77_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRM43102.1"
                     /translation="MAIKGNDTKIRCSFCGKTEDQVRKLIAGPDGAYICDDCVAICSE
                     IIQEEFEDLYGEQNTDEINLIKPKEIKAFLDDYVIGQDEAKKVLSVAVYNHYKRVLSG
                     ASSDIELQKSNILMLGPTGSGKTYLAQTLAKLINVPFAIADATALTEAGYVGEDVENI
                     LLRLIQAADYDIERAEHGIIYIDEIDKITRKSENASITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFICGGAFEGLEKIIETRQDTKSIGFNNPIGHAK
                     DENVGDLLRQVMPQDLTKFGLIPEFVGRVPIVVTLNSLDETALMKILVEPKNSLVKQY
                     KKLFELDGVQLDFKEDALEAIAKKSLERKTGARGLRAIMEDTMMDLMYEIPSDNTTKE
                     CVITKDAVEKKAEPEIIHGEPAPVPRRNMGRRSGRPETA"
     gene            complement(5326..5907)
                     /gene="clpP"
                     /locus_tag="PLN77_02080"
     CDS             complement(5326..5907)
                     /gene="clpP"
                     /locus_tag="PLN77_02080"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRM43103.1"
                     /translation="MSFVPYVIEQTSRGERSYDIYSRLLNDRIIFLGEEVTDVSANLI
                     VAQLLFLESEDPGKDIHMYINSPGGSVSAGLAIYDTMQYIKCDVSTICIGMAASMGAF
                     LLAGGTKGKRMALPNSEIMIHQPSGGAQGQATEIKIVAEQILKTKRKLNEILAANTGK
                     PLEQIEIDTERDNYMSAEEAKAYGLIDNVITNR"
     gene            complement(5932..7218)
                     /gene="tig"
                     /locus_tag="PLN77_02085"
     CDS             complement(5932..7218)
                     /gene="tig"
                     /locus_tag="PLN77_02085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRM43104.1"
                     /translation="MSVQVENLEKNMAKLTIEVPAEELEKALEKAYQKQKKNISVPGF
                     RKGKVPRAMVEKMYGPAVFYEDAVNSLVPEEYSKAAEESKLDIVSRPEINVEQIEKGK
                     AFIFTATVAVKPEVTLGEYKGLEVAKADTTVTDEEVEAELKKEQEKNARTINVEDRPA
                     ADGDTVTLDFEGSVDGVPFDGGAGKDYPLTLGSHSFIPGFEEQLVGAALEEEKEVNVT
                     FPEDYQAEELAGKAAVFKCTIHKIEAKELPALDDDFAKDVSEFDTLDEYKKEIKDNLT
                     KKKEEQAKTEKENAVVDKAVENATMEIPEAMIDTQVENMVDDFARRIQSQGLSMEQYM
                     QFTGATVDSLKEQMKPQAVKRIESRLVLEKVAEAENIQISDEKLDEELAKMAEMYKME
                     LDKFKELVGEYEKEQMKKDLAVQEAVTLMADSAKEA"
     gene            complement(7362..7913)
                     /locus_tag="PLN77_02090"
     CDS             complement(7362..7913)
                     /locus_tag="PLN77_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRM43105.1"
                     /translation="MAKIYVFLADGCEEIEALTPVDLLRRAGEDVCTVSIMGRKEVTG
                     SHKITILADETIEEGEFDDGDMLVLPGGMPGTLNLAGNETLAALIRSYDDQGKKLAAI
                     CAAPSILGVMGILKDKNAVCFPGFEEKLAGANVLDVPAVTDKNVTTGRGMGAATDFAL
                     ELIRVLQGEDKAKEMAEKIVFHC"
     gene            8150..8605
                     /locus_tag="PLN77_02095"
     CDS             8150..8605
                     /locus_tag="PLN77_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRM43106.1"
                     /translation="MEIERKYLLDQLPENLTSYPCKKIEQGYLSTEPVVRIRRSDDEY
                     YLTYKSKGLMVREEYNLPLTKESYLHLREKADGVLISKTRYLLPLTGTDLTIELDVFD
                     APYEGLYLAEVEFPDEASANAFVPPVWFGKDVTYSSDYHNSTLSKGKKL"
     gene            complement(8786..9583)
                     /gene="proC"
                     /locus_tag="PLN77_02100"
     CDS             complement(8786..9583)
                     /gene="proC"
                     /locus_tag="PLN77_02100"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRM43107.1"
                     /translation="MKVGFIGGGNMASAMIGGMIQKGVVSADDILVSVRTEKSVERLT
                     NQFGVQATMENEAVVAGSDLVFLAVKPNQIKNIINLVKNFISPEKIIVSILPGKSLQW
                     LGTEIGKPTKLIRTMPNTPALVGEGMTGVCANDLVTEEELQLVLTLLKSFGRAEVVPE
                     YLMDTVTGVSGSSPAYVFLFLEALADAAVAEGMPRKQAYEFAAQSVLGSAKMLLETGM
                     HPGELKDMVCSPGGTTIEAVAVLERENLRSAVIDATRACIRKSKGVD"
     gene            complement(9795..10802)
                     /locus_tag="PLN77_02105"
     CDS             complement(9795..10802)
                     /locus_tag="PLN77_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43108.1"
                     /translation="MKGKWLTGVLVLAMAAVTLAGCGAKDETKEKVEQVTLNEVAHSI
                     FYAPQYVAIEEGYFADEGIDLTLITGFGADKVLTAMISGEADIGFMGAEATVYAYLEG
                     TADPVVNFAQLTQRAGNFVVGREKDNSFTWEKLKNKEVLGGRKGGMPEMVFEYVLAQK
                     GIDKKDVNINQSIDFGSTAAAFSGGQGDYTIEFEPSATLLEQKGDGYVVASVGTESGY
                     VPYTSYSVKQSYLKTHGATVEKFTKALQKGMDFVQTHTPEEIAEVIAPQFAETELETI
                     TAIVRRYYEQDTWKENLIFEEESFDLLQEILEQAGELEKKAPYKTLVTTKYAEEAAAK
                     G"
     gene            complement(10911..11045)
                     /locus_tag="PLN77_02110"
     CDS             complement(10911..11045)
                     /locus_tag="PLN77_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43109.1"
                     /translation="MAETHYHISKPTACFMLDLNDLKMYEDKIRTKEEQKAKEKQEKK
                     "
     gene            11224..12180
                     /locus_tag="PLN77_02115"
     CDS             11224..12180
                     /locus_tag="PLN77_02115"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRM43110.1"
                     /translation="MKIKSVAVLGAGAVGSYVIWGLSEKKDIRLGVIASGERAKRLKN
                     KGCKINDTVYHPEVWTPEEAHGVDFLIVSLKYGALPGALDDITAVTGENTVIMSLMNG
                     VDSEEIIAEKAGAEHLLHAVIKVASHKENDGYVFNPEATLGIIFGEVSAPYDSERVQA
                     VLDLFSGTGLHYRATDCILEEIWSKFRLNVCNNLPQAILGAGVGCYRDSVHMKAISDG
                     LRAELMAIAEAKGIDISKADVSSGRGSAVPPTARYSTLQDLDAGRHTEIDMFSGALIR
                     MGKELGIPTPYNEFTYHMIKALEEKNDGRFDYSGSEEPLWAK"
     gene            12202..13179
                     /locus_tag="PLN77_02120"
     CDS             12202..13179
                     /locus_tag="PLN77_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM43111.1"
                     /translation="MKEIKWAVLGCGVIANEMAVALQKNGKNIYAVGNRTHEKAAAFA
                     EKYGIGKVYDDFHEMFTDPEVDVIYITTPHNTHLGFMKEAIANGKHILVEKSITLNSD
                     ELDEAIALAKEKGVIIGEAMTIYHMPIYKELKKILDSGRLGRVNLITMNFGSFKEYNM
                     KNRFFNRNLAGGAMLDIGVYALSFLRWFFDSKPDQLLSQVKPAPTGVDEQAGLLLTNQ
                     EGQMASVMLSLHSKQPKRGMVSCENGFIEIMEYPRAWEAKITDASTGESEIIKAGDNA
                     DALAYELADMERAINGDVSAMHLDYTKDVMDLMTSFRRTWGYTYPEEEK"
     gene            complement(13241..13414)
                     /locus_tag="PLN77_02125"
     CDS             complement(13241..13414)
                     /locus_tag="PLN77_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43112.1"
                     /translation="MRGSHGRDDAASILIFAMTANAFVEDRQAAKEAGMNVHIAKPIN
                     AELLKKKIAEYCR"
     regulatory      13571..13682
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13809..14645
                     /gene="thiM"
                     /locus_tag="PLN77_02130"
     CDS             13809..14645
                     /gene="thiM"
                     /locus_tag="PLN77_02130"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRM43113.1"
                     /translation="MLGTCLDNVRKTTPLVHNITNYVTVNDVANILLACGASPIMSDE
                     PEDVEDITSICGGLNINIGTLHRTSIDGMYRAGAKAASLGHKILLDPVGAGASHLRTS
                     TAVGLMEKIPFTVIRGNISEIKTLALGSGTTKGVDADVADKVTDENLDSAVAFAKNFA
                     KDHNCVVAITGAIDLVADADTCYVIRNGRAEMGSITGTGCQLSGMMTAYLVANPDRIA
                     LDEPLKAAAAAVCAMGLAGEIGWSHMKPEDGNSTYRNRIIDAIYHMDGETLEKGAKYE
                     VR"
     gene            14632..15276
                     /gene="thiE"
                     /locus_tag="PLN77_02135"
     CDS             14632..15276
                     /gene="thiE"
                     /locus_tag="PLN77_02135"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRM43114.1"
                     /translation="MKCDKKGLLLYAVTDRSWLNGETLYSQVEKALQGGATFIQLREK
                     NLDQDHFMEEALELKKLCAAYHVPFVINDNVEIAAKMDADGVHVGQSDMEAGDVRAKL
                     GPDKIIGVSAQTVEQAILAEQRGADYLGVGAVFHTGSKADAEDVSHETLTAICNAVKI
                     PVIAIGGITRDNVTELSGSGICGIAVISAIFAQKDIVSATKDLKEQTLRMLENK"
     gene            15392..16171
                     /gene="thiD"
                     /locus_tag="PLN77_02140"
     CDS             15392..16171
                     /gene="thiD"
                     /locus_tag="PLN77_02140"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRM43115.1"
                     /translation="MKTALTIAGSDSSGGAGIQADIKTMITNGVYAMSAITALTAQNT
                     TGVSGIFDVTPEFLAAQLDAVFTDIFPDAVKIGMVSSPALIEVISERLRFYQAKHIVV
                     DPVLVATSGSALASGGVPDLMKKLLFPLAEVITPNIPEGEVLSGIKIQSESDMERAAK
                     EIGDSCGCAVLLKGGHSINDANDLLYHNGTFTWFTGKRIDNPNTHGTGCTLSSAIASG
                     LAKGLSLPDAISAAKEYISGALASMMDLGHGSGPLNHAYRI"
     gene            16197..17504
                     /gene="thiC"
                     /locus_tag="PLN77_02145"
     CDS             16197..17504
                     /gene="thiC"
                     /locus_tag="PLN77_02145"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRM43116.1"
                     /translation="MERNYATQMDAARRGIVTPELKKVAEKEYMAVEDLMPLVASGKV
                     SIPANKLHTCIDPEGIGSMLRTKINVNLGVSKDCKDYDVEMQKVLSAVNMGAEAIMDL
                     SSHGNTQPFRRKLTSECPAMIGTVPVYDSVIHYQRDLDTLTAKDFIDVIRLHAEDGVD
                     FVTLHCGITRKTIDQIRKHKRKMNIVSRGGSLVFAWMCMTGEENPFYEYYDEILDICR
                     EYDVTISLGDACRPGCLADATDVCQIEELVRLGELTKRAWEKDVQVMVEGPGHVPMDQ
                     IAANMKVQQTICMGAPFYVLGPLVTDIAPGYDHITAAIGGAIAAMNGAAFLCYVTPAE
                     HLALPNLDDVKQGIIASKIAAHAADIAKGVPHARDIDDKMGDARRVLDWEAQWDCALD
                     PETAKAIRADRSPEQEDTCSMCGKFCAVRSMNKALNGEHIDIL"
     gene            complement(17591..19081)
                     /gene="glpK"
                     /locus_tag="PLN77_02150"
     CDS             complement(17591..19081)
                     /gene="glpK"
                     /locus_tag="PLN77_02150"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRM43117.1"
                     /translation="MGKYIMALDAGTTSNRCILFDQKGQIVSLAQKEFTQRFPKPGWV
                     EHDANEIWSTMLGVAVEAMQKAGASYEDIAAIGITNQRETTIVWDKKTGEPVYNAIVW
                     QCRRTSEYCDSLMERGLVDRFREKTGLVIDAYFSATKLKWILDEVPGVRERAERGDLY
                     FGTVETWLIWKMTKGKVHVTDYTNASRTMLFNIHTLKWDEDILKELNIPLCMLPEVRP
                     SSCVYGETASTYFGGPIPIAGAAGDQQAALFGQTCYAPGEAKNTYGTGCFLLMNTGEK
                     PVSSTHGLVTTIAWGIGDKITYALEGSIFVGGAAIQWLRDEMKLIESSADSEYMAQKV
                     NDTNGCYVVPAFTGLGAPYWDQYARGTILGLTRGVNKYHVIRATLESITYQVDDVLKA
                     MEADSGMKLSALRVDGGASANNFLMQCQADISQAPVERPCCIETTAMGAAYLAGLAVG
                     YWPDQETVKQNGAIDRTFEPELSKEERDEKMNGWHKAVRYAEGWAK"
     gene            complement(19219..>19656)
                     /locus_tag="PLN77_02155"
     CDS             complement(19219..>19656)
                     /locus_tag="PLN77_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43118.1"
                     /translation="TLEWGARKFTIAPVEGNQSLVPESRTYCVEFGGSSAKEAKVFVN
                     GVEADAEVKEKDGLLTIAVTDVKPQDTVTICLPEDTEIAKNDVMTRAMDLLLHAEISY
                     ITKEQIANLLHKADGKVAILAAELQSMELSNDLRGALLEIITA"
BASE COUNT         4394 a         5401 c         4485 g         5361 t
ORIGIN      
        1 atatgctcca gggcgtgcat ttcatatgcc gttcccgggc tgtatgtgta cgttacatct
       61 tcaagtttca atgacataat gcattcacca gttcttcaat ggtcaaaatt ccctccggga
      121 gatctacacc attttttctc aattcataag ccaacagtgt cacttggggt acatcaagac
      181 ggagttcttt taatttctcc acttcggaaa aaatctccct tggtgtcccc tgcattacca
      241 gcttaccgtt gtccataacg aacacacggt ccgcaaaaac aacctcttcc atgtaatgcg
      301 taatcagaat aaccgtaacg cccttttctt cattcagctt acgcacagcg cgaagaacct
      361 cttttcgtcc gttcggatcc agcatcgcgg ttggttcatc aagaacaatg cattccggct
      421 gcatagccat cactccggca attgcaaccc tctgtttctg acctcccgag agcttattcg
      481 gagagtgggc ccggtatgaa atcatcccgg tcgccgtaag gctctcctcc acccgtttcc
      541 agatttcatc ggtcgggaca cccagatttt ccggtccaaa gccgacatcc tcttccacca
      601 catttccgat gatctggtta tccgggttct ggaataccat accggctttc tggcggatct
      661 tccagacatc ctcctcatct gtcgtcttca tatcgtccac ccacatagtg ccttctgtag
      721 gcaccagcag agcgttgata tgtttcgcaa gcgttgattt tccgcttccg ttatgcccga
      781 gcaccgcgat aaactgacct ttctggattt ccagactgac gtcctcgatc gcttttgtta
      841 cttccggctc ctgtccttcc tcctcgtaac ggaggtattc atacccaagg cctgcagcac
      901 gtatcatacc catgacatta ttccccgctt tcttctgcat ccgagcggat ttcatcaatc
      961 agattccaga tctcctcgcg tccctgtttt gtctctgctg aaaacggaat cacaatcgta
     1021 tctttctcaa cctccagcgt ctcgcggatc agcttgatct gcttctgaag ctggctgcgc
     1081 ttgatcttgt ccagcttcgt cgcaatgatg atcggctgat agccgtttcg aagaatccag
     1141 tcgtacatga tgcgatcgtt gttggacggt gcatgacgga tatcaatcag aagaaatact
     1201 gcgtgcagca tttttgactg atgcagataa tcctcgatca gcttgcccca ttttgcttta
     1261 acctcctcgg aggctctcgc aaaaccgtat cccggcaaat ccaccagata catttcctca
     1321 ttgatgtggt agtagttaat cgtctgtgtt tttcccggct gcgcactggt acgcgccagt
     1381 gatttccgat tcatcagggc attgatgaga gaagattttc caacattgga ttttcccgca
     1441 aatgccactt ccacctgacc ggtatccggc agtttactgg tgattccgca gacgtgctgc
     1501 agctctacct gtttaattac catacttttc ttctcctgtt ttttgcatct tctggcctta
     1561 cttcttttcc acaagtgcca gttttaatac ctgttccatg ctgccgacaa attcgatctg
     1621 cagtccgtct gtgatctccg catccagttc tctgacatcc ggctcatttt tctccggaac
     1681 gagaaccata tggataccgg ctttctttgc cgccagcagc ttctctttta agccgccgat
     1741 cggaagcaca cggccgcgga gcgtaatctc tccggtcatc gcaacgtctg cttttaccgg
     1801 aacctttgtg atcgcggaaa ccatcgccgt cgccatcgta ataccggcag acgggccatc
     1861 ttttggaacg gctccctccg gaatatgaat gtggatgtcg tgtttctcga aaaattcttt
     1921 cggaatctca tactgctctg ccacggaacg aataaagctg atgcccgcct gcgcagactc
     1981 cttcatcaca tcgccgagct ggccggtcag cagaaactct ccttttcccg gcatcacatt
     2041 gacctcgatc tgcagcgtat cgccgccgac tgccgtccag gcaagaccgc gcacaatacc
     2101 aatctccggt ttttcgttgg ccggctggaa ctcatacctc tcttttccga ggaactcttc
     2161 caggttttct ttcgtcacaa caacctcttt tacctgttcc tcgagaatct ttctcgcgct
     2221 ctttctgcag attctgccga tctggcgctc cagggagcgg acaccggcct ctctggtgta
     2281 gccgctgatc acttccatca gcgcctcatc ctcgatcaca agctgtgctt ctttcagacc
     2341 gttcgccttc ttctgcttcg gaacgagatg ttcttttgcg atatggaact tctcgttttc
     2401 cgtatagctt gaaatttcaa tcacttccat acggtccagc agcggacgcg gaatgttctg
     2461 aatatcattt gcggttgcaa taaagagcac ctccgnnnnn nnnnnnnnnn aaaggtcgat
     2521 cggaagctct acgtaatgat cggcaaattt attattctgc tcggaatcga ggacctcaag
     2581 aagtgcggaa gaggtatctc ccttgtaatc tgcacttact ttgtcgatct catcgagcag
     2641 catcagcgga tttttcacct tcgcctgatg gagtcccgcc gcaatgcgtc ccggcatcgc
     2701 gccgatgtag gttcttctgt ggccgcggat ctccgcctca tcccgcacac cgccgagaca
     2761 gatgcggacg tattttttat tcagggctcg cgccacgctt ctcgcaatcg aggtcttacc
     2821 ggttcccgga gggcctgcca ggcagagaat cgggctctcg cctttcttcg tcagggtgcg
     2881 tacggccagg aattcgagga ttctctcttt tactttcgaa agaccgtagt gatcttcctc
     2941 cagaatctcg cgtgctcttg ccagatcttg attgtcacgg gatgccttat cccacggaag
     3001 ccccagcagt gtctcgatat agccgcggat cacgcctgcc tcggacggat tgctggccga
     3061 attttccaga cggtggatct cctcggaaat ccgctctttc acctctttgg aggctttgag
     3121 cttctcaagc tgcttcttat aattttcaat ctcggtccgg gaattgtcct cgccgagttc
     3181 ctcgcggatg actttaagct gctcgcgcag aatataatca cgctggttct tatcgatccg
     3241 ctctttgatt tttccctgaa tctccgaacg gatctcctga atctgaatct cgttggcgat
     3301 catgacgcag agcgtatcat accgctcgga aagctccaca gcctccagca gtttctggcg
     3361 gtttacgtag tcgaggggca gattcatgca gatctggtcc accagctttc caacttcttc
     3421 ggttccgagg atctggcctg ccagttcctt gctgactttc tgcgtctgct cgcagtagac
     3481 ggcaaacagc tcttttaagt tgcgcagcat tgcctcctgc tcgatatcgg aaaactgcat
     3541 ttcgtccgag ccggtaaaca gcgccacctc ggcgcggaga tactcgctct cctcttcctc
     3601 gaagttcagc agttctgcgc gtctttctgc ttctacgaga atccgaagaa catccttggg
     3661 cattcttaca acctgtttga tgaccgcaat tgttccaatt ttatacacat caagcagacc
     3721 cggtacttcc gtatccgggt ctttctgtgt aatcaggaaa attttctggt ctcccagcat
     3781 cgcctcctcg accgcacgaa ccgatttagg tctgctgata tcaaaatgta caatcatatc
     3841 cggcaaaatc gttgttccac gcagcgcaat cgccggtaat acttttgtca cttcttccat
     3901 ttaagtttcc tcctgcgttt ttcaggcggt ttccggtctt ccggatcttc ttcccatatt
     3961 ccgccgtgga acaggggccg gttcgccgtg aataatctcc ggctccgcct tcttttctac
     4021 agcgtccttg gtgatgacgc attcttttgt cgtattgtcc gacgggatct catacatcag
     4081 atccatcatc gtatcttcca tgattgcgcg gagacctctg gcgcctgttt tccgctccag
     4141 ggatttcttt gcaatcgctt cgagtgcatc ttctttgaag tcaagctgta cgccgtccag
     4201 ctcaaacagc ttcttgtact gtttgaccag agaattcttc ggctcaacca gaatcttcat
     4261 cagggccgtc tcatccagag aatttaacgt aacgacaatc ggcacacgtc cgacaaactc
     4321 cggaatcagg ccgaacttgg tcagatcctg cggcatgacc tgacggagca gatctccgac
     4381 attctcatct tttgcatggc cgatcggatt gttgaatccg atggatttcg tatcctgtct
     4441 cgtctcgatg atcttctcga gtccttcgaa tgcaccgccg cagataaaga ggatattcgt
     4501 cgtatcaatc tgaataaact cctgatgcgg atgctttctt ccgccctgcg gcggaacgga
     4561 agctacagtt ccctccagga ttttcagcag tgcctgctgt acaccctcgc cggaaacatc
     4621 tcgtgtgatc gaagcgtttt cggattttct tgtgatttta tcgatctcat cgatgtagat
     4681 gatgccgtgc tcagcgcgct cgatatcata gtctgctgcc tgaatgaggc ggagcagaat
     4741 gttttctacg tcctcgccga cataacctgc ctcggtgagc gccgttgcgt ctgcaattgc
     4801 aaacggaacg ttgatcagtt tggccagtgt ctgtgccaga tacgtttttc cggaaccggt
     4861 cggtccgagc atcagaatgt tgctcttctg cagttcgatg tcggaggatg caccggaaag
     4921 cactcgtttg taatggttgt aaacagcaac ggagagcact tttttcgcct catcctgtcc
     4981 gattacgtag tcatccagaa atgctttgat ttcctttggt ttgatcaggt tgatctcgtc
     5041 ggtattctgc tcgccgtaca gatcctcaaa ctcttcctga atgatctccg agcagattgc
     5101 cacgcagtca tcacagatgt atgccccgtc cgggcctgcg atcagcttgc gtacctggtc
     5161 ttctgtcttt ccgcagaaag agcagcggat cttggtatca ttccctttta tagccatcgt
     5221 tttcttcctt tcaacatatt acaactaaac cttatcgccg gataaggcaa aaggtgccac
     5281 aaaagcctca ttgccggaac tacgcttttg gacacctttc ctctcttatc ggttcgtaat
     5341 tacattatca atcagaccgt atgcttttgc ttcctctgcg gacatgtagt tgtcacgctc
     5401 ggtatcgatc tcaatctgtt ccagcggttt tcctgtattt gccgccagaa tttcatttaa
     5461 tttccgtttg gttttcagaa tctgttctgc aacgattttg atctcggttg cctgaccctg
     5521 tgcaccgccg gatggctgat gaatcatgat ttcagagttt ggcagagcca ttctctttcc
     5581 tttggtaccg ccggcaagaa ggaatgctcc catgctggca gccataccga tacagatcgt
     5641 ggatacatca catttgatgt actgcatcgt atcgtagata gcaagtcctg cgcttacaga
     5701 acctcccgga ctgttgatat acatgtggat atcttttccc ggatcttcag actccaggaa
     5761 cagaagctgt gcaacgatca ggtttgccga tacgtcagtg acttcctctc ccagaaaaat
     5821 aatacggtcg ttcagcagtc ttgaataaat atcgtaactt ctctctcccc tgcttgtctg
     5881 ctcgatgaca taaggtacaa aactcattca attgcctctc tttccgaggt cttacgcctc
     5941 ttttgcgctg tcggccatga gagttactgc ttcctgaaca gcgagatctt tcttcatctg
     6001 ctctttctca tattcgccaa cgagctcttt gaatttatcc agctccattt tgtacatttc
     6061 agccatttta gccagctctt cgtccagctt ctcatcgctg atctggatgt tttctgcttc
     6121 tgcaactttc tcaagtacca gtctgctctc gattctctta acagcctgcg gtttcatctg
     6181 ctcttttaag gaatcaacgg tagcgcctgt aaactgcatg tactgctcca tggacagacc
     6241 ctgggactgg attcttcttg cgaaatcgtc aaccatgttc tctacctgcg tatcgatcat
     6301 agcttccgga atctccattg ttgcgttctc tactgcttta tctacgacag cgttctcttt
     6361 ttctgtctta gcctgctctt cttttttctt ggtcaggttg tctttgattt cttttttgta
     6421 ctcatccaga gtatcgaact cggaaacgtc ttttgcgaag tcgtcatcaa gtgccggaag
     6481 ctcttttgct tcgattttgt ggatggtgca tttgaatact gctgcttttc cagcaagctc
     6541 ttctgcctga taatcctctg ggaaagtaac gtttacttcc ttctcctctt ccagagctgc
     6601 gccaacgagc tgctcttcga atcccggaat gaaggaatgg gagccaaggg tcagcggata
     6661 atcttttcca gcgccgccgt cgaacggaac gccgtctaca gaaccttcga agtcaagtgt
     6721 aacggtatct ccgtctgctg ccggacgatc ctctacattg atggttcttg catttttctc
     6781 ctgctctttt ttcagctcag cttctacttc ttcatctgta acggttgtgt cagcttttgc
     6841 tacttccaga cctttgtact cgccaagggt aacttccggt tttactgcta cagttgcggt
     6901 aaagatgaaa gctttgcctt tttcgatctg ctctacgttg atctccggac gggatacgat
     6961 gtccagtttg ctctcttctg ctgctttgga atactcttcc ggaacaaggc tgtttacagc
     7021 atcctcatag aatactgccg ggccatacat tttttctacc atagctctcg gaacttttcc
     7081 tttacggaag cccggaacgc tgatgttctt tttctgtttc tggtaagctt tctccagtgc
     7141 tttttctaat tcttcagccg gaacctcaat ggtaagtttg gccatattct tttctaaatt
     7201 ttcgacctgt acactcattt tatcaatttc ctccttgatt tatctgaaaa gcggcagaaa
     7261 acctgcacac ctctccattc tacagtcatt tatcgagtat agcatactct tttcaaaaac
     7321 gccagtgatt tttttatatt tctgtggttt ttaggctttt tttagcaatg aaatacgatt
     7381 ttttccgcca tttcttttgc tttgtcctct ccctgcagaa cacggatcag ctccagtgcg
     7441 aagtcagtgg ctgctcccat gccgcggccg gtggtcacat ttttgtcggt gactgccggt
     7501 acatcgagta cgtttgcgcc tgccagcttt tcttcaaatc ccgggaagca gacggcgttt
     7561 ttatctttta agatgcccat cacaccaagg atgctcggcg ctgcgcagat ggctgccagt
     7621 ttctttccct gatcgtcata ggaacggatc agcgctgcga gcgtctcgtt tccggccaga
     7681 tttaatgtac ccggcattcc gcccggaagt accagcatgt ctccgtcatc aaactctccc
     7741 tcttcgatgg tttcatctgc aagaatggtg attttgtggg atcctgttac ttcttttctt
     7801 cccataatag aaacggtgca tacgtcttct ccggctctgc gcagcagatc caccggggtc
     7861 agtgcctcaa tctcttcaca tccgtctgcc agaaatacat aaatctttgc catggttttg
     7921 tcctcctgtg ttgtatgatc agtctcgtgt gttttgtcac gtgatattct ctgcttttct
     7981 ttgtaacaaa ccgccgctat tataccacat ttttccgcgg aatgcttgca tttcataaaa
     8041 atttcaggac tttttttcgc aaaaagccgg atttcgctta acatcacggg aaaagcatgg
     8101 taaactcttg aaaaaagcag tgacgcaatc acgagaagga gtctctttta tggaaatcga
     8161 acgcaaatac cttctggatc agcttccgga aaatcttaca agctatccat gtaaaaaaat
     8221 cgagcagggc tatctttcca ccgaaccggt tgtccgcatc cgccgctctg acgacgaata
     8281 ttatctgacc tacaagtcaa aagggctgat ggtgcgtgaa gagtataatc ttccgctcac
     8341 aaaggaatcc tacctgcatc tgcgggaaaa ggcagacggc gtactcatct caaaaacacg
     8401 ctatctgctg ccgcttaccg gaacggatct gacgatcgag ctcgatgtct ttgatgcgcc
     8461 gtatgaaggc ctttacctcg cagaggtgga gtttccggac gaagcatccg caaacgcctt
     8521 tgttcctccg gtatggtttg gaaaggatgt aacctattct tccgattatc acaacagtac
     8581 gctcagtaaa ggaaaaaaac tataaattac cagaaaaaat cccggtgcct tttccacggc
     8641 tgccgggatt tttccgctcg tttgtttttt tactcttttt tgtgcttgct ttttgcttgt
     8701 tttggtttgt ttttttgtgc atacattctt tattttgctg ctttcacatt agcgtcattc
     8761 tgtcaaagcg gcggtgctgc tccttctaat ccactccctt tgatttccgg atgcaggctc
     8821 gtgttgcatc aataacagca cttctcaggt tctcccgctc cagtacggca accgcttcga
     8881 tggtggttcc gcctggcgag cagaccatgt cttttaattc tcccgggtgc attcctgtct
     8941 cgagaagcat tttggcgctt ccaaggacag actgtgcagc aaattcatat gcctgttttc
     9001 tcggcattcc ttcggctacg gccgcatctg caagagcctc caggaaaaga aatacgtacg
     9061 ccggcgagct tccgctgaca cctgtgacgg tatccatcag atactccgga acaacttctg
     9121 cgcggccaaa gcttttcagg agggttaaaa cgagctgcag ctcttcctcc gtcacgagat
     9181 cattggcaca gacgccggtc attccttctc cgacgagtgc tggtgtgttt ggcatggtgc
     9241 gaattaactt tgttggtttt ccgatttctg tacccagcca ttgtaaagac ttaccgggaa
     9301 gaatcgatac gataatcttc tcgggtgaga tgaagttttt gacgaggttt attatatttt
     9361 ttatttggtt tggctttact gccaggaata caagatcgga tccggctacg accgcttcgt
     9421 tctccatagt cgcctggact ccaaactggt tggtcaagcg ttccacactt ttctccgtgc
     9481 ggaccgatac cagaatgtca tctgcagata caactccttt ttggatcatt ccgccaatca
     9541 tggcactcgc catgttgccg ccgccgataa aacctacttt catgtctgtt cgcctccgta
     9601 tgcctgtgtt gtaaaaaata tatgcgacac ccatagtatg caacaaaatt tacttttttg
     9661 caatactttt tctctatttt tcttcttttc ttttacaatt ttctttattt ttttcgaatg
     9721 aaatgtcgct gtttgtcgac gaatgtcgaa aagtgtcttt ttctgtcttt ttttgctttt
     9781 ctattccgcc ggaatcagcc tttggcggcg gcctcttcgg cgtattttgt tgtcacgagt
     9841 gtcttgtacg gtgccttttt ctccagctct cccgcctgct ccagaatctc ctgcagaaga
     9901 tcaaaacttt cttcctcaaa gatcagattt tctttccacg tatcctgctc atagtagcgg
     9961 cgcacgatcg cggtgatggt ttccagctcg gtttctgcaa actgcggcgc aatcacttct
    10021 gcaatctcct ccggtgtgtg cgtctgcacg aagtccattc ccttctgcag tgccttcgtg
    10081 aatttttcta cggttgcgcc gtgcgttttc agataactct gtttgacgct gtacgacgta
    10141 tacggcacat acccggattc cgttccgacc gatgccacga catagccgtc gcccttctgc
    10201 tcgagcagtg tcgcggacgg ttcaaattca atcgtataat ccccctgtcc gccggagaac
    10261 gcggcagctg tggaaccaaa atcgatgctc tggttgatgt ttacgtcttt tttatcaatc
    10321 cctttctgcg ccagaacata ctcaaagacc atttccggca taccgccttt tcttccccct
    10381 aatacctctt tgtttttcaa tttctcccag gtaaacgaat tgtctttctc tcttcccaca
    10441 acaaagtttc ccgcccgctg cgtcagctgc gcgaagttca caaccgggtc tgcggttccc
    10501 tcgaggtacg cgtagacggt tgcctccgcc cccataaacc cgatatccgc ctcgccggaa
    10561 atcatcgccg tcagcacttt atctgcacca aatcctgtta tcagcgtcag atcaatgccc
    10621 tcgtccgcga aatacccctc ctcaattgcc acgtactgcg gggcgtaaaa aatggagtgg
    10681 gcaacctcat ttaatgtcac ctgctccact ttttcttttg tctcatcttt tgccccgcat
    10741 cccgccagcg tcactgccgc cattgccagt acaagcacac ctgtcagcca ttttcctttc
    10801 atcaacagat tctcctgttt tttcattcta accttagaat atgatttccc ggtaaaaatg
    10861 cgtgcaaaaa acgaaggaga aacgttcgaa tttcgtttct ccttcgtttc ttatttcttc
    10921 tcttgttttt cttttgcctt ctgttcttct ttcgtccgga ttttgtcttc gtacattttc
    10981 agatcgttca gatccagcat gaagcaggcc gtcggtttgg aaatgtggta atgtgtttct
    11041 gccatctcct cttgccgcat ctgcgcgttt cttttatatt tttcattcta caatctacct
    11101 tatcgctccg tcaacacatt taggcttcct tttccgaaat tttctatttt gaaaaacaaa
    11161 ttgaggatct ccgcaaaatt tgttatagta aggataattc aaacagttac aaaggaggct
    11221 attatgaaaa taaaatcagt tgctgttctg ggcgccggag cggttgggtc ttatgtgatc
    11281 tgggggcttt cggagaaaaa ggacatccgg cttggcgtta tcgcgtccgg ggaacgggcg
    11341 aagcgtctga aaaacaaagg atgcaaaatt aacgatacgg tttatcaccc ggaggtatgg
    11401 acaccggaag aggcacacgg cgtggatttt ctgatcgtct cgctcaaata cggcgcactg
    11461 ccgggggctc ttgacgatat tactgccgtt accggcgaga atactgtgat catgagtctg
    11521 atgaacggcg tggacagcga ggagatcatc gcggaaaaag ccggtgcgga gcatctgctt
    11581 catgctgtta tcaaagtagc ttctcataaa gagaatgacg gctacgtttt caaccctgag
    11641 gcgacgctcg gcatcatttt cggcgaagtt tcggcgccgt acgacagtga gcgtgtgcag
    11701 gctgttctgg atctgttctc cggcacaggg ctgcattacc gggcaaccga ttgtatttta
    11761 gaggaaatat ggagcaaatt ccggctgaat gtctgcaata acctgccgca ggctattctc
    11821 ggtgccgggg tcggctgcta ccgcgacagt gtgcatatga aggcgatcag cgacggactc
    11881 cgcgcagaac tgatggcgat tgcggaggca aagggaattg atatcagcaa ggcggatgtc
    11941 tcctcgggac gcggaagtgc ggttccgccg acggcgcgct actcgacgct ccaggatctc
    12001 gatgcgggac gccatacgga aattgatatg ttctcgggtg cgctgatccg catgggaaaa
    12061 gagcttggaa ttccaacgcc ttacaatgaa tttacgtacc atatgatcaa agcactggaa
    12121 gagaaaaacg acggacggtt tgactacagc ggaagcgaag aaccgctctg ggcgaaatag
    12181 atacgatagg agaatctcac gatgaaagaa atcaaatggg ctgtactcgg atgcggtgtg
    12241 attgcaaatg aaatggcggt cgcactgcag aaaaacggaa agaatattta cgcggtcggc
    12301 aaccggaccc atgagaaggc ggctgctttt gcggagaaat atggaattgg aaaagtttac
    12361 gatgatttcc atgagatgtt taccgatccg gaggtcgatg tcatttatat cacgacgccg
    12421 cacaacacgc atcttggctt tatgaaagag gcgattgcca atggaaaaca cattctcgtt
    12481 gagaaatcga ttacgctgaa cagtgacgag ctcgacgagg ccatagcgct tgcgaaagaa
    12541 aaaggtgtca tcatcggaga ggcaatgacg atttaccata tgccgatcta caaagagctg
    12601 aaaaaaattc tcgactccgg ccgtctcggc agagtcaatc tgatcacgat gaacttcggc
    12661 agtttcaaag agtacaacat gaagaaccgc ttcttcaacc ggaatctcgc aggcggcgcg
    12721 atgcttgata tcggcgtata cgcactctcc ttcctccgct ggttcttcga ctccaagccg
    12781 gatcagctcc tttcccaggt aaagcccgct ccgaccggtg tggacgagca ggccggcctc
    12841 cttctcacca atcaggaagg acagatggca agtgtgatgc tctctctcca ctccaaacag
    12901 ccgaaacgcg gcatggtttc ctgtgagaac ggatttatcg agatcatgga gtacccgcgt
    12961 gcctgggaag caaaaatcac agacgcttcc accggcgaat ccgaaatcat caaagccggt
    13021 gacaacgccg atgcactcgc ctatgagctc gccgacatgg aacgcgccat caatggagat
    13081 gtatctgcca tgcacctcga ctacacgaag gacgtcatgg atctgatgac ctccttccgc
    13141 agaacctggg gctatacgta cccggaggaa gaaaaataag attcgcacat caaaagcccc
    13201 tgcccggcac acatcagatg ccgggcaggg gcacttcttt tcacctgcaa tactctgcaa
    13261 ttttcttttt cagtaattcc gcattaatcg gctttgcgat atgcacgttc atgccggcct
    13321 cttttgcggc ctgccgatct tcaacaaagg cgttggcggt catggcgaaa atcagaatgg
    13381 aggcggcatc atcacgtcca tgacttccac gcatatttta tacttcattt tacgctgata
    13441 ttattttctc tgtcaaccca aataacatta catttccaat attttgcatg tcttttttat
    13501 ttttctgaat tttttttaaa tcttccgttt ttctcttgca caattgtttt tatacgtata
    13561 taattgtctg cagaaagcaa aggggagctc gcgtgcgaag cgggctgaga gtaggatgca
    13621 tctcctagac cctgaacctg atttgggtaa tgccaacgta gggagatacg atatgcacat
    13681 tttctgcaga tatacctcca cgtatatctg cttttttaat ctctatgaac acatcatcaa
    13741 caggttcttc cagtatcaca agggaatttg cttctgcaaa aatactcaca caggaggaac
    13801 aaaaaattat gttaggcact tgtcttgaca atgtcagaaa aacaacccct ctcgtacaca
    13861 acatcacaaa ttacgttacg gtaaacgatg tcgccaatat tctgctggca tgcggcgcaa
    13921 gcccgatcat gtccgatgaa ccggaggatg tcgaggatat cacctccatc tgcggcggtc
    13981 tgaacatcaa catcggaacg ctgcacagaa cgagcatcga cggcatgtac cgtgccggtg
    14041 caaaagcagc ttcgctcggc cacaaaatcc tgctcgatcc agtcggcgca ggcgcaagcc
    14101 acttacgtac ttcgaccgca gtcggactga tggaaaaaat cccgttcacc gtcatccgcg
    14161 gaaatatttc cgaaatcaaa acactggcac tcggaagcgg aacgacaaag ggtgttgacg
    14221 cagacgttgc cgataaagta acagacgaaa acctcgacag cgccgttgct tttgcaaaaa
    14281 actttgcgaa agaccacaac tgtgttgtcg ccatcacagg agcgatcgac ctcgttgccg
    14341 atgctgacac ctgctacgtg atccgcaacg gacgcgccga gatgggaagc atcaccggaa
    14401 caggctgcca gctctccggc atgatgaccg cttacctcgt tgccaacccg gaccgcatag
    14461 ctttggacga gccgttaaaa gccgctgctg ccgctgtctg cgcgatggga cttgccggcg
    14521 agatcggctg gagccatatg aaaccggaag acggcaactc cacctaccgg aaccgcatca
    14581 tcgacgccat ctaccacatg gacggcgaga cgctggagaa aggtgcaaag tatgaagtgc
    14641 gataaaaaag gcctgctttt atatgctgtg acagaccgca gctggctgaa cggcgagaca
    14701 ctgtacagcc aggtggaaaa ggctctgcag ggcggcgcaa cgtttatcca gctccgcgaa
    14761 aaaaatcttg accaggatca ctttatggaa gaggcactgg aactgaaaaa actctgtgcc
    14821 gcataccatg ttcctttcgt catcaacgat aacgtggaaa tcgcagctaa gatggatgcg
    14881 gacggcgttc atgtcggcca gagcgatatg gaggccggcg atgtccgtgc aaagcttggc
    14941 ccggacaaga tcatcggcgt ttcggcacag accgtggaac aggccattct cgccgagcag
    15001 cgcggggcag attacctcgg cgtcggcgcg gtcttccaca ccggttccaa ggcggatgcg
    15061 gaggatgtca gccacgaaac gctgactgct atctgcaatg ccgttaaaat ccctgtcatc
    15121 gccatcggcg gcatcacacg cgataacgtg acggagcttt ccggcagcgg catctgcggc
    15181 atcgccgtca tcagtgccat ttttgcacag aaagacattg tttccgcaac gaaggatctg
    15241 aaagaacaga ctctgcgcat gctggaaaac aaataatttt acgtaaccat tcatcctcac
    15301 accaggcggc agactggggg caccactttc tttgtcgcct gtccaaaaag aaaagtaatg
    15361 caaaggagta attctcatgg acgcgaaaaa catgaaaaca gcattgacaa tcgcgggaag
    15421 tgattccagc ggaggcgccg gcattcaggc agacatcaaa accatgatca caaacggagt
    15481 ttacgccatg tcggcaatta ccgcactcac cgcccagaac accaccggag tttccggtat
    15541 tttcgatgta acaccggagt ttctggccgc acagcttgac gccgtcttta cggacatctt
    15601 cccggacgcc gtcaaaatcg gtatggtatc ctccccggca ctgatcgaag tcatctccga
    15661 acggcttcgc ttctaccagg ccaaacacat cgttgtggac ccggtcctcg tcgctacaag
    15721 cggctccgcg ctcgcttcgg gcggcgtgcc tgacctgatg aaaaaactgc tctttcctct
    15781 ggccgaagtc atcacaccga atatcccaga gggagaagtt ctctccggca tcaaaatcca
    15841 gtcggaaagc gatatggaac gcgccgcaaa agagatcggg gattcctgcg gctgtgccgt
    15901 tctgttaaag ggcggccaca gtatcaatga tgccaacgat cttttatatc acaacggcac
    15961 atttacctgg ttcaccggaa aacgcatcga caatccgaac acacacggca ccggctgtac
    16021 actctccagc gccatcgcat cggggctcgc aaaggggctt tcccttccgg atgccatttc
    16081 cgccgcaaaa gagtatattt cgggcgcact cgcgagcatg atggatctcg gccacggctc
    16141 gggaccgctg aaccacgctt accggatctg attttgaaaa taaaggagaa caacagatgg
    16201 aacgcaatta cgcaacacag atggacgccg cacgccgcgg cattgtaaca ccggagctga
    16261 aaaaagtcgc agaaaaagag tatatggccg tggaagacct gatgccgctc gttgccagcg
    16321 gaaaggtttc cattccggcc aataagctgc acacctgcat cgacccggag ggcatcggct
    16381 cgatgctgcg cacgaaaatc aatgtaaatc tcggtgtttc gaaagactgt aaagattacg
    16441 atgtggagat gcagaaggta ctctccgcgg tcaatatggg cgcagaagcc atcatggacc
    16501 tgtcgagcca cggcaatacg cagccgttcc ggcggaaact gacctccgaa tgtccggcca
    16561 tgatcggcac cgtgccggta tatgacagtg tgatccatta ccagagagac ctggatacgc
    16621 tgaccgccaa agattttatt gacgtcatcc gcctccatgc cgaggacggt gtcgatttcg
    16681 tcaccctgca ctgcggcatc accagaaaaa ccatcgatca gatccgcaaa cacaaacgga
    16741 aaatgaatat cgtttcccgc ggcggctccc tcgtgttcgc ctggatgtgc atgaccggcg
    16801 aggagaatcc gttctacgag tattacgatg agattctcga tatctgccgt gaatacgatg
    16861 tgaccatttc tctcggcgac gcctgccgtc ctggctgcct tgccgacgca acggatgtct
    16921 gccagattga ggagctggtg cgcctcggcg aactcaccaa acgtgcctgg gaaaaagacg
    16981 ttcaggttat ggttgaggga ccgggccacg ttccgatgga tcagattgcg gccaacatga
    17041 aagtacagca gaccatctgc atgggcgctc cgttctatgt cctcggacct cttgtgaccg
    17101 atattgcacc gggctatgac cacatcaccg ctgccatcgg aggcgcaatt gccgctatga
    17161 acggcgctgc tttcctctgc tacgtaacgc cggcagagca tctggctctt ccgaacctcg
    17221 atgacgtaaa gcagggcatc atcgcaagca aaatcgccgc acacgctgcc gacattgcca
    17281 aaggcgtgcc gcatgcgcgt gatattgacg acaagatggg agacgcccgc cgcgtgctcg
    17341 attgggaagc acagtgggac tgtgcactgg acccggagac tgcaaaagcc atccgtgctg
    17401 accgctcgcc ggaacaggaa gatacctgct cgatgtgcgg taaattctgt gccgtgcgaa
    17461 gcatgaacaa agctttaaac ggcgaacata tcgacattct ttaacatcag tagacaagaa
    17521 gtcacagtac gcgaaaagga acggttttca ttacgaaacc gttccttttc tgcatttcca
    17581 aaatcttttt ctatttcgcc catccttcgg cgtaacggac tgctttgtgc catccgttca
    17641 tcttctcatc gcgctcctct ttcgagagct ccggctcaaa tgtacggtca atggctccgt
    17701 tctgttttac cgtctcctga tccggccagt agccgacggc gagtcccgca agataagctg
    17761 ctcccatcgc ggtcgtctcg atgcagcacg gccgctccac cggcgcctgg ctgatgtccg
    17821 cctggcactg catcagaaaa ttgttggcgc ttgctccgcc gtccacacgc agtgcggaga
    17881 gcttcatgcc ggaatctgcc tccatcgctt ttaagacatc atccacctga tatgtgatcg
    17941 actctagcgt tgcgcggatc acatggtatt tgttgacgcc gcgcgtcagg ccgagaatgg
    18001 ttcctctcgc atactgatcc cagtacggtg cgccaaggcc ggtaaacgcc ggcacgacgt
    18061 agcagccgtt cgtgtcgttc accttctgcg ccatgtactc ggaatccgcc gaggactcga
    18121 tcagcttcat ctcatcgcgc agccactgga ttgcggctcc gccgacaaaa atggatccct
    18181 cgagcgcata cgtaatcttg tccccgatac cccatgcaat cgtggtaaca agaccgtgtg
    18241 tcgaagaaac cggcttttct cccgtgttca tcagcaggaa gcagcctgtt ccgtatgtat
    18301 ttttcgcttc gcccggcgca tagcaggtct ggccgaacag cgccgcctgc tggtctcccg
    18361 ctgctcccgc aatcggaatc gggccgccga aatatgtgga tgccgtctcc ccgtagacac
    18421 agctcgacgg gcggacttcc ggcagcatac agagcggaat gttcagctct tttaagatat
    18481 cctcatccca tttcagtgta tggatattga acagcattgt acgggacgcg ttggtgtaat
    18541 cggtgacatg tacctttccc tttgtcatct tccagatcag ccacgtctcc actgttccga
    18601 aatagagatc gcctcgctcc gcccgctcgc gcacgcccgg aacttcatct agaatccatt
    18661 tcagcttcgt ggctgaaaaa tacgcatcaa tcacaagacc tgttttctcc cggaaacggt
    18721 caaccagtcc gcgctccatc agggaatcgc agtattccga tgttctgcgg cactgccata
    18781 caattgcatt gtagaccggc tctcccgtct ttttgtccca gacgatcgtc gtctctctct
    18841 ggtttgtgat gccgattgcc gcgatatcct cgtaggacgc gcccgctttc tgcattgcct
    18901 ccacggctac accgagcatc gtggaccaga tttcattggc atcgtgctcc acccagcccg
    18961 gtttcgggaa acgctgggta aactccttct gcgcaaggct tacgatctgt cctttctgat
    19021 caaacagaat acagcggttg ctcgttgtgc cggcatcaag tgccatgatg tattttccca
    19081 cgtttaaaaa tcctcctgtc tcacatttca agaagagagc ctgacagaat ccactgccac
    19141 gctctcttcc ttttctttgt atgtatcgta tcacagaatt ctttttctgt aaatcaaatc
    19201 gtgtttttct gcagcaattt atgccgtaat gatctccagc agcgcaccgc gcagatcgtt
    19261 tgaaagttcc atgctctgca actctgcagc cagaattgct acctttccgt ccgctttgtg
    19321 aagaagattt gcgatctgct ctttggtgat gtagctgatt tcggcgtgca gcagcagatc
    19381 catcgcacgt gtcatgacat cgttctttgc aatctccgta tcttccggca gacagattgt
    19441 cacggtatcc tgcggtttta catctgttac cgcgatcgta agaagtccgt ctttctcttt
    19501 gacttctgca tccgcttcca cgccgtttac aaagaccttt gcctcttttg cactacttcc
    19561 accgaattct acgcaatatg ttctgctttc cggaacaagg ctctgattgc cctcaaccgg
    19621 tgcaattgta aattttcgtg caccccattc gagcgt
//
LOCUS       DAOMUF010000016        19444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_482_length_19444_cov_4.450926, whole genome shotgun sequence.
ACCESSION   DAOMUF010000016 DAOMUF010000000
VERSION     DAOMUF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 19444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19444
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_482_length_19444_cov_4.450926"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..1372
                     /locus_tag="PLN77_02160"
     CDS             8..1372
                     /locus_tag="PLN77_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM43119.1"
                     /translation="MKTTTKQTHLYSNTDLRKLLIPIIIEQLLSSLMGTVDTMMVSNV
                     GSAAISAVSLVDSINILVIQALSALAAGGAILCSQYLGSEKRDGAIRSAQQVLFVMTV
                     LSVALSAGCLIFRVPLLRVIFGAVEADVMKNSQVYFLFTLLSFPFIGLYDAGASILRA
                     QNNSRSPMVISVISNFMNIGGNAILIFGFHMGVEGAAISTLISRIFCAVVVLWKLRDE
                     SKPIFVKQYLSIRPDFGLIKKILFIGIPSGIENSMFQFGKLAIQSTVSTLGTVAIAAQ
                     AMTNILENLNGVAAIGVGIGLMTVVGQCLGAGRKDEAIYYIKKLCWLSEVVIVASCLL
                     VFVLTKPITMIGGMEAESAKLCMQMMIFITIVKPLCWVMAFVPGYGMRAAGDVKFSMI
                     TSCCTMWLCRVSLCIYLCRVWGFGPIAVWIGMAADWSLRSILFTIRFKSGKWLNHHLI
                     EEKK"
     gene            1711..2025
                     /locus_tag="PLN77_02165"
     CDS             1711..2025
                     /locus_tag="PLN77_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM43120.1"
                     /translation="MEPIYLSIQQKETGSRIKSLLRESGYTVRDIQNAMGFENPQAVY
                     KWISGKCLPSLDNFLILSRLLNTSIEDILVIDGDIPRFRSKYREIRERLNRWYNDKIV
                     IL"
     gene            2364..3572
                     /locus_tag="PLN77_02170"
     CDS             2364..3572
                     /locus_tag="PLN77_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRM43121.1"
                     /translation="MDQIKIKGKVNTAICYARVVEEEAIEQIRRMCDYPMTEGSRIRI
                     MPDVHAGKGCTIGTTMTIRDKAVPNVVGVDIGCGMYTVNLGRAELDFAKIDEAAHVIP
                     SGMNVWEGRQERFDLMTLACSRELKDTKRLERSLGTLGGGNHFIEIDEAADGTKYLVI
                     HSGSRNLGKQVAEYYQNLAVRLDRGYDEYLEKREELIRTYKEQGRRKEIQEALKQLQW
                     KPDEAQMPEDLCYLTGKYLEAYLHDVEICQQFARRNREKMAEILLERTGLTGTDAFHT
                     IHNYIDTNEMILRKGAIAAHKGERVLIPINMRDGSILAVGKGNPEWNDSAPHGAGRLM
                     SRKKAKETLALEEYRKSMEGIYTTSVNEHTLDEAPMAYKSLDDILDIVRASVDVVEVL
                     RPVYNFKASE"
     gene            3622..3876
                     /locus_tag="PLN77_02175"
     CDS             3622..3876
                     /locus_tag="PLN77_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM43122.1"
                     /translation="MMAIPSIDMEATGRNITRLRQQAGLTVRDLQEIFGFTTPQAIYK
                     WQHGTAMPAIDNLVVLAAVLHVSIDEIVIVETRELVLASA"
     gene            4135..4674
                     /locus_tag="PLN77_02180"
     CDS             4135..4674
                     /locus_tag="PLN77_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43123.1"
                     /translation="MTKKKIIGLCIGAVVLAVAAAGVKEGSWLMKHRRMQNEFTHEMN
                     AFTTKTNAELIQPTIVAEDDIYGWFFHEEDDGLYMNLYYRIKNESNQHEIASSEFPVK
                     IKKELLADDKVWSRTTRTAKNQVHAEYLEGEDDMTVSFDVYYQCEGSCGEYFADITVT
                     DSETGKLYYSSEAENEAAE"
     gene            4683..6068
                     /locus_tag="PLN77_02185"
     CDS             4683..6068
                     /locus_tag="PLN77_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRM43124.1"
                     /translation="MAEKQSKNKWHTPAERIMMLGFAAVILLGTILLCLPVSAADGKS
                     VYWLDALFTATTSVCVTGLVTVPTATTWSTFGKIVILGLIQFGGLGIMACLTMVFLIL
                     RRKISLQSRKLIQDTYNLPVLKGSVGIVRRLLIGTATVEIAGAVLYSFWFVPEYGFWK
                     GIGYSIFHAVSAFCNAGIDLVGEASFAPFVTNPLINFTTMGLILLSGLGFPVWWEVME
                     RVQELVKGKRPRKNFVRGFTLHTKLVLTTTMILVFGGALLILALDWNHAPSLGSLKPA
                     QKVMAAFFQSVTTRTAGFETIPQADFSDSSAMVSMVLMFIGGSPMGTAGGVKTTTVAI
                     LVVLVASYIRGDSDTVAWGRKVMEENIRTAVVIFFFVLTTVFTATVLLISVTGSPLLD
                     CTYEIISAVATVGLTRSLTPTLPFMGKVIVILVMFMGRIGPVTLAVALRRRTGRKDVD
                     IQRPEQRILIG"
     gene            6116..6772
                     /locus_tag="PLN77_02190"
     CDS             6116..6772
                     /locus_tag="PLN77_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRM43125.1"
                     /translation="MSKQYAVIGLGKFGFSVATTLAQAGKEVLAVDKDAEPVQEIADL
                     VTYAARADITDSRVFASLGISNMDVVIVGISENMESSILATLQAKEAGVPLVIAKCMS
                     QMHANILKKVGADRVVMAESETGIRLGKSLISGGFQDFFMLSDSFSMVELPVPEAWTG
                     HNLEELDLRKKYSINVIAVKDGEDIRVTVNPKTLLRAEEVLILIGENKELAKLMKNRK
                     "
     gene            6784..7554
                     /locus_tag="PLN77_02195"
     CDS             6784..7554
                     /locus_tag="PLN77_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRM43126.1"
                     /translation="MITSSSNAQVKNIIALNKKAKERREQDVFVAEGWKMFQEAPREW
                     LKKVYVSESGSKMHEIPSDGTEYEVVDDRVFQSMCDTKTPQGVLSVIRMPHYTEEEVM
                     DNGKTPLLMVLEDLQDPGNVGTIIRTAEGAGVTGIIMSRGTADIFNPKTIRSTMGSIY
                     RMPFLIVDDAVGFVKGLKARKICTYAAHLHGVHSYREEDYTKGTAFLIGNEGNGLTDA
                     MAEAAECLIRIPMEGKVESLNAAIASAVLMYEAHGQRA"
     gene            7730..9199
                     /locus_tag="PLN77_02200"
     CDS             7730..9199
                     /locus_tag="PLN77_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HRM43127.1"
                     /translation="MSEKKNISKKELRRRKRKRALMIKTGILCVLLVIFGIGIWALAG
                     GTEKIQQKAQEKEDQKTAEVDGSVSSDSTGSAEAPTTKAQIMAEADALAQTYDYDGAI
                     EKLQSVEGAATDADIITKVAEYTSTRDACVRVNVNEVTHIFYHSLVVDPQKAFYQDNA
                     QTAGFCQWMTTVDEFNAITQQMYDRGYVMVSINDLVKKTVDDDGTVHYEEGDIYLPEG
                     KKAFVLSLDDLSYYHSYDGRGIASKMVVGDDGKPTCEYIQDDGTVVTGAYDCIPLMDQ
                     FIEAHPDAVYHNARGTVALTGYDGILGYRTDGDYKTREDLTDDQVAWLDAHPDFDWDK
                     ECEEAKKVADAIKADGWTFASHTWGHIRVGDKPIETIQADTEKWLTYVAPLIGGSDII
                     IFAHGQDLSDWHDYTMDNEKFAYLKSQGFNIYCNVDSSQYFVQVRDNYLRMGRRNLDG
                     YRLYQNLYGGGEDRTSDLFDSASVIDPQRPTDPSLYNLG"
     gene            complement(9290..11056)
                     /locus_tag="PLN77_02205"
     CDS             complement(9290..11056)
                     /locus_tag="PLN77_02205"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRM43128.1"
                     /translation="MKPASLHRRILFPLLLCGGLLFALLFWYFSPFFSPGENRRFSAY
                     VEERFHSEVTSSAITLHYTLADPASRGIAPGTASFGTVSIPDRTSYDALLQSVETTLT
                     SFHRNRLSAENQITLDLLLYELNCEKMPGSTSLLAEPLGPSLGIQAQLPILLAEYPFR
                     TQQDIADYLHLLQDLPRYFSDLIALEQEKSRQGVFQNDLAVDGIIRQCSAFLADADTP
                     ESHLLHTVFQNKLQASSLFSEAERNLCLQTHEKLLTSCVFPAYRSLSDALSSLRGKGT
                     QNAGGLSRQEGGLSYYAYLLRSRVGVDASPEELTRLLTQQLADQCAEMQIILDQNPSL
                     SLSEQALVLPFSTPEEMLADLQNAVSEDFPVLSGIAYEVKTVDDSLKDFLSPAFYLTP
                     PADVNSPNSIYLNPADEMTGIELYSTLAHEGFPGHLYQTVSFSRSDPLLIRHLYAPSG
                     YVEGWATYVESYACRYAARNLDANTQALCRLWYLNRSVHLILCSLLDLGIHSQGWLLS
                     DASAFLAQFGITDASAAASLYQYILETPANYLKYCVGSLSFSQLLSEVQKQEGDSFSL
                     IGFHEKLLRLGPLPFPLVEKYL"
     regulatory      11176..11380
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11661..15449
                     /locus_tag="PLN77_02210"
     CDS             11661..15449
                     /locus_tag="PLN77_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43129.1"
                     /translation="MKGNSSKFLALLLSASMIMSPTASVLADTQEKAAQTESSAAAEE
                     APQSEETAETAEPETTPAETESSESTAETPAEEPTETPAAPAEDQTPAGSDETETPSA
                     DEDASEDTAEDTAKDGEETADTTEKEPEAADETSQDAGKETPAKEETKDAVKENTSPA
                     AEQPKTEVKETTQNEASNTAEKKETKAAEAAKPADGEYETSAEATGSMFRVISSRLIV
                     KDGKLKASILLSGTGYDYLYTGTAAEAAAADEKSWIAPTGSETYTDTKTGAEKTGYRF
                     EIPVEELDKQMDVAVRSAKKKTWADKTVTIHSTDAIPTVTPEPTETPSENPGEDSSNK
                     PASVEAPVTKEDGSVFKMFVISEATMINKGDKLVLTLKTNNVSFDKLYLGKNDDLAKT
                     PAIQGMQEANGGYSFTFEVDADKAGTTVPIVLGKKDGTWYTKQQLYLAVPGSKTPEVT
                     PIPEPTETPVPTATPTPSETPVPTATPTPEPAKVENGIYSGTAETGAAMFKVVGVELT
                     VKDGDMKARITLSGVAYDYLYMGTPEEAAAADKSAWIAGVGTVDYTTADGETKTGMQY
                     DIPVAALDQALVVSSHSAKRDKWYARTITIASSSLKKTADLPADETNKVADGIYSAEA
                     TTNAAMFKVVGVKLTAKNGKMTAVITLSGSGYDYLYMGTGKDAAAESGAWIASTGKVD
                     YTLDGETKTGMQFEIPVEALDQELAVAAHAVKSGKWYDRAITIASSSLKKTGDLPSDD
                     NKGDTPKPTLVPTNTPTVSPSETPVVKPTSTPTPAPTQKPADTKPEQESKYESDLSGG
                     TSKVDSATTLADGVYTPDGFSFSGGSGKVTISCSKITVTNGQAYATIAFSSPYYGYVK
                     ANGNKYMANVSGGSSIFTIPVELNKNNTIIGMTTRMSVAHEISYNIFISLAAAKKGGS
                     TSSSTGFVKADGTISTISTDNSKLDTTAPEIMGLEYKAETKLAYAEYFKMYHYDEGIT
                     LLEIDMTKDTERDPEKQTEDDKKAEDTDTAAAEDTAAATDEEEAAAASDSKKTGTEKA
                     KDLTEKTKELYEGNVVKYLIVPKDVEIPAGLDKDMIVIQLPVEKAYVASEDALKLLDE
                     QLDAAESIKAVGMEQKDCRIENIAKAMEDKKISFDGAFDDLDYKALVKDEIDFAILPS
                     EFLPGNAKDEEDADAADETADTKAEDQKDDKDDKTTDEKADEDKTPEELLKEENERLS
                     DTAERLATLTIPMLVDRSADEKTDLAKAEWLKVYGVIFGCEDQANELFQQMVKAEENK
                     "
     gene            15529..16893
                     /locus_tag="PLN77_02215"
     CDS             15529..16893
                     /locus_tag="PLN77_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43130.1"
                     /translation="MTGKNERKLKKWAALFLAAAMSLTACGSTASTGSSTSSASGTSA
                     VSESSTSTASAADVSESQTESTSVSESTAESESTSEATAESSTSGSDTASADGFATTD
                     DVSDAPDITGITIESKMVLNYAECFNVYYCTDGYKLIDVKDGAQYLLVPDGKEAPDDL
                     DSDVIVIHQPLERIYMAATSPMALFDAIDSLDTIRLSGETADNWYVQDAVDAMNAGTM
                     IFAGKYSEPDYELLVSENCDLAIESTMILHSPKVQEMIEMLDIPVFIDRSSYESQPLG
                     RTEWIKLYGAMLDKEEEAADFFASQASVVENLKDFQNTEKTVAFFYINTSGAAVVRNP
                     EDYISNMIELGGARNAFRDLQDDSGKTSVPITMEQFYDTAEDADYLIYNASIDSTVKT
                     LDDLLAKDSMFADYKAVKEGNVWTTEKSMYQATDKIAQFTNDINLLVTGGDPDQMVFL
                     RKLS"
     gene            16905..17966
                     /locus_tag="PLN77_02220"
     CDS             16905..17966
                     /locus_tag="PLN77_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRM43131.1"
                     /translation="MSEKKNMTGKENSFHMENFRRRSRYAAVFLVLILAFAAITVLNI
                     NSGNVNISIPEIFRILLRRTGEGKEYNIIWKIRLPRILMAAILGGALSLSGFLLQTFF
                     ENPIAGPFVLGISSGAKMVVALTMIFYLEWARNITSYTMIVAAFLGSLLSVGFILLMS
                     RRIKNMAGLLVGGIMIGYICSAVTDFVVTFAEDSDIVNLHGWSQGSFSGMSWSNVAIS
                     AAVIGITFVITFLMAKPIGAYQLGEAYAQSMGVNIKVFRIALILLSSILSACVTAFAG
                     PISFVGIAVPFLVKQSLGTSRPLVVIPGTFLGGAVFCMMCDLIARMAFAPLELSISTV
                     TSIFGAPVVIFMMLRRKRG"
     gene            17973..19070
                     /locus_tag="PLN77_02225"
     CDS             17973..19070
                     /locus_tag="PLN77_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43132.1"
                     /translation="MNEYYFHLDHLTVGYDKKPLIKDICIGIEKGEIVTLIGPNGSGK
                     STILKSITRQLKLVGGNVEFDGKNLHELSFRELSTKMAVVLTERMKPELMTCHDIVAT
                     GRYPYTGRLGMLTREDEEKVEKAMRAVHAEELGGRDFNAISDGQRQRVLLARAICQEP
                     EVIILDEPTSFLDIRHKLELLAILRRMAKEKGITVIMSLHEIDLAQKISDKIICVKGD
                     AISHFGAPETIFREDIIRELYEIDNGSFDPCFGSIELPRPEGTPRVFVLAGGGTGIPV
                     FRKLQKENVPFAAGVLYTNDIDYQLARILAMETVTEAPFQEISDEAFARACELMKSCE
                     RVIDTGVPVGMCNRRIEELRAEAKRLGKLAE"
BASE COUNT         5472 a         4494 c         5349 g         4129 t
ORIGIN      
        1 aaaaacaatg aaaactacaa caaaacaaac tcatttatac agtaacacgg atcttcgcaa
       61 acttttaatt ccgatcatca ttgaacagtt actttcttcc ctgatgggaa ctgtcgacac
      121 gatgatggtc agcaatgtcg gttctgccgc aatttcggca gtctcgctgg tggattccat
      181 taatatctta gtcattcagg cgctttctgc gctggcggca ggaggagcga ttctctgttc
      241 gcagtatctt ggaagcgaaa aacgggatgg agcaatccgt tcggcacagc aggtgctttt
      301 tgttatgaca gttttgtccg tcgcattatc cgcgggctgc ctgattttcc gtgttccgct
      361 gctgcgcgtg attttcgggg cagtggaagc ggatgttatg aaaaattcac aggtgtattt
      421 cctgtttacg ctgctgtcat tcccgtttat cggtctttac gatgcgggcg catccattct
      481 gcgggcgcag aacaacagcc gcagtccgat ggtgatttcg gtgatttcca actttatgaa
      541 catcggcgga aatgccattc ttattttcgg cttccatatg ggtgtcgaag gagccgcgat
      601 ttcgacttta atttcgcgta ttttctgtgc ggttgttgta ctgtggaagc ttcgggatga
      661 aagcaaaccg atttttgtga agcagtatct ttccattcgg ccggactttg gtttgatcaa
      721 aaaaatactg tttatcggta tcccatcggg aatcgagaac agtatgttcc agtttggaaa
      781 gctggcgatt cagtccactg tgtccacact tggcacggtt gccattgccg cgcaggcgat
      841 gacaaacata cttgagaacc tgaacggtgt cgctgcgatc ggtgtcggca tcggactgat
      901 gacggtagtc ggtcagtgtc tcggcgcggg ccggaaggat gaagcaatct actatattaa
      961 aaaactgtgc tggctgtcag aagtggttat cgttgcaagc tgtctgctgg tatttgtgct
     1021 gacaaaaccg attacgatga tcggcggtat ggaggcagag agtgcaaagc tctgcatgca
     1081 gatgatgatc tttatcacga ttgtaaagcc gctctgctgg gtcatggcgt ttgtcccggg
     1141 atacggcatg cgggctgccg gggatgtaaa attctccatg atcacgtcct gctgtacgat
     1201 gtggctgtgc cgtgtgagtc tctgtattta cctgtgccgc gtatggggat tcggcccgat
     1261 tgcggtctgg atcggtatgg cggcagactg gagcctgcgg tccattctct ttaccatccg
     1321 gttcaaatca ggaaaatggc tgaatcatca tttgatagaa gaaaagaaat aggaagttcg
     1381 ccacacgctc tagacatctt cttgcggtaa aaaggaaagc gcatatgcaa gagagtcgtt
     1441 gagatgagat ttttgaccgc ggctggaagc cgtaccggat caatcacctg agctggtgga
     1501 aagaggagct tgcatccgaa gaagagatgg aagagggacg gaacaatctt gcagcacacg
     1561 acaatcggaa tgtagatgaa aagaaaatcc ttttgtacga acagatcatt cgggttgtgt
     1621 agaaacagga agaaaaaact ggatttttcc agtaccggat gataagataa aactgtgttt
     1681 cgggatggct taaacatatg gaggtgcagg atggaaccaa tttatttgtc aatccagcag
     1741 aaagaaactg gcagccgcat caaatccctt cttcgtgaaa gcggatacac cgtgagagac
     1801 attcagaatg ccatgggttt tgaaaatcca caggcggtct ataaatggat ttccggcaag
     1861 tgcctgccaa gtctggacaa ctttctcatt ttaagcagat tattaaacac aagcatcgaa
     1921 gatatcctcg tcattgacgg ggatattcct cgtttccgaa gcaaatacag agaaatccga
     1981 gaaagattaa accgctggta taatgacaaa atcgttattt tgtgatacag tgaagaaaaa
     2041 gcagaaaaag aaccggttaa aaaggttttg gaaaggaaaa acagatgtca gcaggaatac
     2101 gttttggcta ccgcaattta gaacataccc cagaagcaac gtttgtcgtc aaacagcgtg
     2161 gctttgtttt gatatgttca attgagaaaa gcgacagaac ggattctgtg gaatatgata
     2221 cgggaggatc aaattagacg accgcatcag acaactggat acgaacgaag aggagccgct
     2281 tatcttatgt tgaacacatg ggataggtgg ctttttctat taaattttta tatcagaaca
     2341 aattgagaag ggagaaatcc ggaatggatc agatcaaaat caaaggaaaa gtaaatacag
     2401 ccatctgcta tgccagggtc gtagaggagg aagccatcga gcagatccgc cgcatgtgcg
     2461 attatccgat gacggaaggc tcgcggatcc gcattatgcc ggatgtgcat gcgggaaaag
     2521 gatgtacgat cggaacaacg atgaccatcc gcgacaaggc agtgccgaac gtcgtcggcg
     2581 tggacatcgg gtgcggcatg tacaccgtaa atcttggaag agccgaactt gattttgcaa
     2641 aaatcgacga ggcggcacat gttatcccgt ccggcatgaa tgtctgggag gggagacagg
     2701 aacgttttga tctgatgaca cttgcctgca gccgggaatt aaaagacaca aaacgcctgg
     2761 agcggtcgct tggaacctta ggcggcggca accattttat cgagatcgat gaagccgccg
     2821 acggcacaaa atatctcgtc atccattccg gctcgcggaa tctcggaaaa caggtggcag
     2881 agtattacca gaatctggca gtgcggctgg accgcggtta tgacgagtac ctggaaaaac
     2941 gcgaggagct catacgcacc tacaaagagc agggccgcag aaaggaaatc caggaggcgt
     3001 taaaacagct gcagtggaaa ccggacgaag cgcagatgcc agaggatctc tgctatctga
     3061 ccggaaaata tctggaagcc tatctgcacg acgtggagat ctgccagcag tttgcacgcc
     3121 ggaaccggga aaaaatggcg gaaatcctgc tggaacgaac cggccttacc ggcaccgatg
     3181 cgttccacac gattcataac tatatcgaca caaacgagat gatactccga aaaggcgcaa
     3241 tcgcagcgca caaaggagaa cgagtgctga ttccgatcaa tatgcgcgac ggcagtattc
     3301 tcgccgtcgg aaaaggaaat ccggagtgga atgattctgc cccgcacgga gccggacggt
     3361 tgatgtcgcg caaaaaagcg aaggaaaccc tggcactgga ggaataccgg aagagcatgg
     3421 agggaatcta tacgacttcc gtcaacgaac ataccctgga cgaggcgccg atggcgtaca
     3481 aatcgctgga tgatattctc gatatagtcc gtgcatcggt cgatgtcgtg gaggtgctgc
     3541 gtcccgttta caatttcaaa gcatcagaat agaggcggag gaaaccagta aaattcctcc
     3601 gcaagaagga ggaagaaaaa aatgatggca attccgtcaa ttgatatgga agcaacagga
     3661 agaaacatta caagactgcg gcagcaggca ggccttaccg tccgcgacct gcaggaaatt
     3721 ttcggtttca ccaccccgca ggcaatctac aagtggcagc acggcaccgc catgccggca
     3781 atcgacaacc tggtcgtgct ggcagcggta ctgcatgtgt cgatcgatga gatcgtgatt
     3841 gtggagacaa gagagcttgt gcttgcgagt gcataaacct gagaacatat tttacgcaac
     3901 tttaacacaa tcttccctca atttcatgta gaataaaaag cacagattcc gtagcttttc
     3961 tacaaaccgc agcagccggc ttatggtagc agaagcactc ggtgaggcag aaacagcagg
     4021 ctggatcggg ttggctccgt tgactttata tgtgcaaact gataagatac tcataagtat
     4081 gttcttaggg gtatcttatt tttgtgtttc gaaaagaaaa ggggaaatta aataatgaca
     4141 aaaaagaaaa tcattggcct ttgcatcggc gctgtcgttc ttgccgtggc tgcggccggt
     4201 gtgaaggaag gcagctggct gatgaagcat cgccggatgc agaatgagtt tacgcatgag
     4261 atgaatgcat tcacgaccaa aacaaacgct gagctgatcc agccgacgat tgttgcggag
     4321 gatgatatct acggctggtt tttccatgag gaagatgacg ggctgtacat gaatctctat
     4381 taccggatca aaaacgagtc caatcagcac gaaattgcat ccagtgaatt tcctgtgaag
     4441 atcaaaaagg agcttcttgc ggatgataaa gtgtggagca gaacgacgcg gaccgcgaaa
     4501 aatcaggttc acgcggaata cctggaagga gaagatgaca tgacggtttc gtttgacgtc
     4561 tattaccagt gtgagggttc ctgcggggaa tattttgcgg atattaccgt gacggatagt
     4621 gagaccggaa agctctacta cagcagtgaa gcagaaaatg aagcggcaga ataggaggag
     4681 cgatggccga aaaacaaagc aaaaataaat ggcacacacc ggcggagcgg attatgatgc
     4741 tgggatttgc ggcggtaatc ctgctgggaa cgattcttct ttgcctgccg gtttctgcgg
     4801 cggacggaaa aagcgtttac tggctcgacg ccctgtttac cgcaacgaca tctgtctgcg
     4861 tgacgggcct tgtcaccgtt ccgacggcga cgacatggag tacctttgga aaaattgtga
     4921 tcctcgggtt gattcagttc ggaggtcttg gcatcatggc gtgcctgacg atggtgtttc
     4981 tgatcctgcg ccggaaaatc agtctgcaga gccggaaatt aattcaggat acctataatc
     5041 tgccggtttt aaagggcagt gtgggcatcg tgcgccggct tctgattgga acagcaaccg
     5101 tagagattgc gggggcggtt ctgtatagct tctggtttgt gccggagtat ggcttctgga
     5161 agggaatcgg ctacagtatt tttcacgccg tttccgcttt ctgtaatgcg ggaatcgatc
     5221 tcgttggcga agcctctttt gctccgtttg taacgaaccc gctgataaac tttacgacga
     5281 tgggactgat tcttttgagc ggtcttggtt ttcccgtctg gtgggaagtt atggagcggg
     5341 tgcaggagct tgtgaaggga aaacggcccc gtaaaaattt tgtgcgagga ttcacgcttc
     5401 atacgaaact ggttttaacg acaaccatga ttctggtgtt cggtggagca ctgctgattc
     5461 ttgcactgga ctggaatcac gcgccatcac ttggcagcct gaaaccggcg cagaaggtca
     5521 tggcggcttt ttttcagtcc gtgacgacaa gaacggcagg ctttgagacg atcccgcagg
     5581 ctgatttctc ggacagctct gcaatggtaa gtatggttct catgttcatc ggtggctccc
     5641 cgatgggaac ggcaggtggt gttaagacga caacggttgc cattctggtt gtgctggttg
     5701 cctcctatat tcgcggagac tcagatacgg tggcctgggg gcggaaagtg atggaagaaa
     5761 atatccgtac cgctgtcgtc attttctttt ttgtactgac gacagtcttt actgccaccg
     5821 tgctgctgat ttccgtaacc ggaagcccgc tgctggattg tacgtatgaa attatttctg
     5881 cggttgcaac ggtgggcctc acccgttccc tgacgccgac gcttcctttt atgggaaagg
     5941 tgattgttat tctggttatg tttatgggaa gaatcgggcc ggtgacgctg gctgtcgcac
     6001 tgcgccgcag aacaggaaga aaagatgtcg atattcagag accggagcag cggattctga
     6061 ttggatagtt gggaaaataa aaaacaggac agatttcaga aaacgaggaa aagaaatgag
     6121 taaacagtat gcagtcattg gacttggaaa gtttggtttt tctgtagcaa cgacacttgc
     6181 gcaggcagga aaagaagtgc ttgccgttga taaagacgct gagccggtac aggaaattgc
     6241 cgatcttgtt acctatgcgg cgcgtgcaga catcacggac agtcgtgtgt tcgcatctct
     6301 tggcatcagc aatatggatg tggtgatcgt tggcatttcc gaaaatatgg agtcgagcat
     6361 cctcgccacc ctgcaggcaa aagaggcggg cgtgccgctc gtcatcgcaa agtgcatgag
     6421 ccagatgcat gccaatattt tgaaaaaagt cggcgcggac cgcgtcgtga tggcggagag
     6481 tgagacgggc atccgcctcg gaaaaagcct gatttccggc ggcttccagg acttcttcat
     6541 gctgtcggat tcgttctcca tggtagagct tcccgtgccg gaagcgtgga cggggcataa
     6601 cctcgaggag ctggatctgc ggaagaaata cagtatcaac gtaatcgccg tcaaggacgg
     6661 ggaagacatc cgtgtcacgg tcaatccgaa gacgctgctc cgcgcagagg aagtgctgat
     6721 tctgatcggc gaaaacaaag aacttgccaa attgatgaaa aacaggaaat gaggggacca
     6781 ttcatgatta caagcagcag caatgcacag gtaaaaaaca ttattgcgct gaacaaaaaa
     6841 gcaaaagaac gccgggagca ggacgttttc gtggcggagg gctggaaaat gttccaggaa
     6901 gcgccgcggg aatggctgaa aaaagtatat gtgtctgaga gcggcagcaa aatgcatgaa
     6961 ataccgtccg acgggacaga atacgaagta gtggacgacc gcgtgttcca gtccatgtgc
     7021 gacacgaaga cgccgcaggg tgttttaagt gtgatccgca tgccgcatta caccgaagag
     7081 gaggtgatgg acaacggaaa gacaccgctt ctgatggtac ttgaggatct gcaggaccct
     7141 ggaaatgtcg gaacaatcat ccgcaccgca gagggcgcag gagtcaccgg catcatcatg
     7201 agccgcggta ccgcggatat cttcaatccg aaaacgatcc gctcgacgat gggctcgatc
     7261 taccgtatgc cgtttctgat cgtcgacgat gcggtcggat ttgtaaaagg cttaaaagcg
     7321 cggaagatct gtacgtacgc ggcacatctg cacggcgtgc attcctaccg cgaggaggat
     7381 tacacaaaag gaacggcatt cctgatcgga aatgagggaa acggcctgac tgatgcaatg
     7441 gcagaggccg ccgagtgcct gatccgtatt ccgatggaag gaaaagtgga atcgttaaac
     7501 gcagcgattg catccgccgt tctcatgtat gaggcacacg gccagcgcgc ataaaagaaa
     7561 gagcgttacc ggttttctta catttctctt aacctgccgg aaacctactc gacagaaccg
     7621 gtattttgag ctaaaataga aagagcagag tccgggctga atttctgtca gtccggtaaa
     7681 actgtaatat ttgccagaaa agcaaaagct gttttgggag gaaggaaaaa tgagtgagaa
     7741 aaaaaacatc agtaaaaagg agctccgaag aagaaagcgg aagcgtgcgc tgatgatcaa
     7801 aacaggaatt ctatgtgttc tgcttgtcat cttcggcatt ggaatctggg ctttggccgg
     7861 aggaacagaa aaaatccagc agaaagcaca ggaaaaagag gatcagaaaa cagcagaggt
     7921 ggacggttcc gtatcgtcgg acagcacggg aagtgctgaa gcgccgacaa cgaaagcaca
     7981 gattatggcg gaagccgatg cacttgcgca gacatacgat tacgatggag ccattgaaaa
     8041 gctccagagc gtagagggcg cagcaaccga cgcggatatc atcacaaaag tagcagagta
     8101 caccagcacg agagatgcct gtgtgcgcgt caacgtcaac gaagtaaccc acattttcta
     8161 tcattctctt gtagtcgatc cgcagaaagc tttctatcag gacaacgcac agaccgcagg
     8221 cttctgccag tggatgacca ccgtggatga gttcaacgcg attacccagc agatgtacga
     8281 cagaggatat gtcatggttt cgatcaacga tctcgtgaaa aagaccgtag atgatgacgg
     8341 aaccgttcat tacgaggaag gggacattta cctgccggag ggcaagaaag cattcgttct
     8401 ttctctggat gacctttcct attaccactc ttacgacgga cgcggcattg ccagcaagat
     8461 ggtagtcggc gacgacggaa aaccgacctg cgaatacatt caggatgacg gaactgttgt
     8521 gaccggtgct tacgactgca tcccgctgat ggatcagttt atcgaagcgc atccggatgc
     8581 cgtataccat aatgcgcgcg gaaccgtagc actgaccggc tacgacggaa tcctcggata
     8641 ccgcaccgac ggtgattaca agacaagaga agatctgacg gatgatcagg tagcatggct
     8701 tgatgcacac ccggatttcg actgggataa agagtgcgag gaggcgaaaa aagtagccga
     8761 tgcgatcaaa gcagacggct ggacattcgc aagccatacc tggggccata tccgtgtcgg
     8821 cgacaaaccg atcgagacga tccaggcgga taccgagaaa tggctgacgt atgttgcacc
     8881 gctcatcggc ggaagcgata tcatcatctt tgcacatgga caggatcttt ccgactggca
     8941 cgattacacg atggacaatg agaagtttgc ttacttaaag agtcagggct tcaacatcta
     9001 ctgcaacgta gattcctccc agtatttcgt tcaggtaaga gacaattacc tgcgtatggg
     9061 acgaagaaac ctggatggat accgtctgta ccagaacctc tacggaggcg gcgaggatag
     9121 aacaagcgat ctgtttgatt ctgcctctgt tattgatccg cagcggccga cagatccatc
     9181 tctttacaat ttaggttaaa gaattttgaa aatgaaaaag atctcacaga actttctatt
     9241 atttatagaa gaaagtctgt gggatctttt tttcagcagg gcgaaccgtt tacagatatt
     9301 tttctacgag cggaaacggc agaggcccga gacggagcag tttttcatgg aaaccgatca
     9361 atgagaatga gtcaccttcc tgtttctgca cttccgagag gagctgcgaa aacgagagcg
     9421 atccgacaca gtatttcaga tagttcgccg gtgtctcgag aatatactga tataaagagg
     9481 cagcggccga cgcatccgtg atgccgaact gcgccagaaa ggctgacgca tccgagagga
     9541 gccatccctg gctgtggatt ccgagatcca gcagcgagca gaggataaga tgcaccgagc
     9601 ggttcagata ccagagacgg cagagcgcct gcgtgtttgc atcgagatta cgtgcggcgt
     9661 agcggcaggc atatgattca acgtaggttg cccatccttc cacataaccc gacggcgcgt
     9721 agagatggcg gatcagaagc gggtcggagc gcgagaacga gacggtctgg tacagatggc
     9781 cgggaaagcc ctcgtgggca agtgtggaat acagctcgat gccggtcatt tcatccgcgg
     9841 gattcaggta aatggagttg ggtgaattga catcggcggg cggtgtgaga taaaaggcgg
     9901 ggcttaaaaa atccttgaga gagtcatcca cggtctttac ttcgtatgcg atacccgaaa
     9961 gaacgggaaa atcttcagaa acggcattct gcagatcggc aagcatttcc tccggcgtgg
    10021 agaacggcag cacgagcgcc tgctcggaga gggagagcga cggattctga tcgagaatga
    10081 tctgcatttc ggcacactgg tccgcaagct gctgtgttaa aaggcgggtc agctcctccg
    10141 gtgaagcgtc aacaccgacg cggctgcgga gaagatacgc atagtaggaa agtccgcctt
    10201 cctgccggga aagaccgccg gcgttctgcg ttcctttgcc gcggagtgaa gaaagagcat
    10261 cggaaagaga gcggtaggcg ggaaaaacac aggaggtaag gagtttttca tgcgtctgca
    10321 gacagagatt cctttccgcc tcagaaaaca gtgaagacgc ctgcagttta ttctgaaaaa
    10381 cggtgtggag cagatggctt tccggtgtat ccgcgtcggc gagaaaagca ctgcactggc
    10441 ggataatgcc atcgaccgca aggtcattct gaaacacgcc ctgccgggat ttttcctgct
    10501 cgagtgcgat gaggtcggag aaatagcgcg ggagatcctg cagaagatgg aggtagtcgg
    10561 cgatatcctg ctgtgtgcgg aacgggtact ccgcgagcag aatgggaagc tgtgcctgaa
    10621 tcccgagcga cggaccgagc ggttccgcaa gaaggctggt gctgcccggc attttctcgc
    10681 agttcagctc gtagagcaga agatcgagcg tgatctgatt ttccgcggag aggcggtttc
    10741 gatgaaagga tgtgagcgtt gtttccacac tctgcagcag ggcatcatac gaggtgcggt
    10801 cagggatgga aaccgtgccg aacgaggcag tgccgggggc aattccgcgg gaagccggat
    10861 cggcgagggt atagtggagc gtgatggcag aagaggttac ctcggagtgg aagcgttcct
    10921 ccacatacgc ggaaaaccgg cggttctcac caggggaaaa gaacggggaa aaataccaga
    10981 acagcagggc gaaaagaagt ccgccgcaca gaagcagagg gaacaggatg cggcggtgca
    11041 gagaagctgg tttcatagaa taggctccgg tgcggacagt tatcataaag tatgtaaaaa
    11101 atgctgattt tatgcgcttt ttccgtggcc tttcatttga ctttccaaaa cggctttgct
    11161 ataatcagta acgaatgaaa taagggactt ttcagtgcgg aacggatttg aaaaagaatc
    11221 cgcgaaggta aaagggaatc aggtggaaat cctgagcgat ccggtcactg taagcaggga
    11281 gtgttagctg cagaaatgcc attgtgcgaa acgcatgaga aggcgcggct gccgtgaaga
    11341 tctgcgagtc aggaaacctg ctggaaaggt tagtgaatcg cttccgataa aagcatatat
    11401 attcatggaa agatgtacga agtaaaatcg gacatctgct tttttgtcgc catgcgtccg
    11461 aaaaagagct ccggtggaga aactcttttg cagaaaaagc agatgtctgt ttgtcttttc
    11521 gcagaaaaaa tgtgtcctca aaaacaagat tacttgcagg gagacatcat tgacggggac
    11581 aggcaccgcc cagctgcctg aaaaagtcag acggaatccg gagcagatgc gcgaaggaga
    11641 tccgaagaaa aggagaagtt atgaaaggaa attcaagcaa atttctggca ctgctgctgt
    11701 cggcgtccat gatcatgagc ccgactgcca gcgtattagc agatacgcag gaaaaagcag
    11761 cacagacaga aagcagcgca gccgcagagg aagcgccgca gagcgaagaa acagcagaga
    11821 cggcagaacc ggaaacaaca ccggcagaaa ccgagagcag tgaaagcact gcagagactc
    11881 cggcagaaga gccgacggaa acaccggccg cgcctgcaga ggatcagaca ccggcaggat
    11941 ctgacgagac cgagacacca tccgcagatg aggatgcttc ggaagatact gcagaggaca
    12001 ctgcaaaaga cggtgaagag actgccgata ccacagaaaa agagccggaa gcagcagatg
    12061 agacttccca agatgctgga aaagaaacac cggcaaaaga agaaaccaaa gatgctgtaa
    12121 aagagaatac cagtccggcg gcagaacagc cgaaaacgga agtaaaagaa acaacccaaa
    12181 atgaggcatc caatacagcc gagaagaaag aaacaaaagc agcggaagca gcaaaaccgg
    12241 cagatggtga gtatgagact tctgcagaag caaccggaag catgttccgt gtgatttctt
    12301 ccagactcat cgtaaaagat ggaaaactga aagcatccat tctgctgagt ggaaccggct
    12361 atgattacct gtatacggga acagcagcag aagctgccgc agcagatgaa aagagctgga
    12421 ttgctccgac aggaagcgaa acctatacgg atacgaagac cggtgcagaa aagaccggat
    12481 atcgttttga aattccggtg gaagagcttg acaaacagat ggatgttgcc gttcgctcag
    12541 ccaagaaaaa aacatgggct gataaaaccg ttacgatcca cagcaccgat gcaatcccga
    12601 cggtaacacc ggaaccaacc gagactccaa gcgaaaatcc aggtgaggat tcctcgaata
    12661 agccagccag tgtagaagca ccggttacca aagaggacgg cagcgtattc aaaatgttcg
    12721 tcatctccga ggcaacaatg atcaacaagg gcgataaact cgttcttaca ctgaaaacaa
    12781 acaacgtatc atttgataaa ctgtatcttg gaaagaacga tgaccttgca aaaacaccgg
    12841 ctatccaggg aatgcaggaa gcaaacggcg gttattcctt tacatttgaa gtagatgcgg
    12901 acaaagccgg cacaaccgtt ccgatcgttc ttggaaagaa agacggaacc tggtacacca
    12961 aacagcagct ttatcttgca gttccgggaa gtaaaacacc ggaagtaact ccgataccgg
    13021 agccgaccga gactccggtc ccgactgcaa ccccgactcc gagtgagact ccggttccaa
    13081 ccgcaactcc aactccggaa ccggcgaaag tagaaaacgg tatctacagc ggaaccgcgg
    13141 agaccggcgc agctatgttc aaagtagtcg gcgtagagct gaccgtaaaa gacggcgaca
    13201 tgaaagcacg catcacgtta agtggtgttg cctacgatta tctgtacatg ggaacaccgg
    13261 aagaggcagc tgccgcagac aagagtgcat ggattgcagg cgtcggcacc gttgattaca
    13321 caacagcaga cggcgagacg aaaacaggaa tgcagtatga tattccggta gcagcactcg
    13381 atcaggcact tgttgtttct tcccattctg caaaacggga taaatggtat gcaagaacca
    13441 ttactattgc atcttccagt ctgaaaaaga ccgcagatct tccggcagat gagaccaata
    13501 aagtagcaga tggtatttac agtgcagagg caacaactaa tgcagcaatg ttcaaggtag
    13561 tcggcgtaaa actgaccgcg aaaaacggaa aaatgacagc agttatcacc ttaagcggaa
    13621 gcggctatga ttatctgtat atgggaaccg gaaaagatgc ggcagcagag agcggcgcat
    13681 ggatcgcttc caccggaaaa gtggactaca ccttagacgg tgaaaccaaa accggaatgc
    13741 agtttgaaat cccggtagaa gcactggatc aggaacttgc tgtagcagct catgcagtaa
    13801 agagcggcaa atggtacgac agagccatca cgattgcatc ttccagcctg aaaaagaccg
    13861 gagaccttcc gtcggatgac aacaagggcg atactccgaa accaaccctc gttccgacca
    13921 ataccccaac cgtatctcca tctgagacac cggttgtaaa accgaccagc accccgactc
    13981 cggcaccgac ccagaaaccg gccgatacca aaccggagca ggaatccaaa tatgagtctg
    14041 acttaagcgg cggcacatcc aaagtagaca gtgcaaccac actggcagac ggcgtttaca
    14101 caccagacgg attctccttc tccggcggat ccggaaaggt aaccatctcc tgcagcaaga
    14161 ttactgtaac gaacggacag gcttatgcaa cgatcgcatt cagcagtccg tactacggat
    14221 atgtaaaagc aaacggaaat aaatacatgg caaatgtcag cggcggaagc agcatcttca
    14281 ccattccggt tgagctgaac aagaacaata ccatcatcgg tatgaccacc agaatgtccg
    14341 ttgcccatga gatttcctac aacatcttca tctccctggc agcagcaaag aagggcggca
    14401 gcacttcttc cagcacagga tttgtaaaag cagacggaac aatctccacg atcagcaccg
    14461 ataactccaa gctggatacc accgctccgg agatcatggg actggaatac aaagcagaga
    14521 caaaacttgc ttacgcagag tacttcaaga tgtatcatta cgacgaaggc atcaccctgc
    14581 tggagatcga tatgacaaaa gataccgagc gtgatccgga gaaacagacc gaggacgaca
    14641 agaaagcaga agataccgat accgcagcag cagaggatac cgcagcagca accgacgagg
    14701 aagaagcggc agcagcatcc gattccaaaa agaccggcac tgagaaagcg aaagatctga
    14761 ccgaaaagac aaaagagctg tacgaaggca atgttgtcaa atatctgatc gttccgaaag
    14821 atgttgagat tccggcagga ctggacaaag atatgatcgt gatccagctt ccggttgaga
    14881 aagcatacgt agcatctgag gatgccctga aacttctcga cgagcagtta gacgcagcag
    14941 agagcatcaa agcagtcggc atggagcaga aagactgccg gatcgaaaac attgcaaaag
    15001 caatggaaga taaaaaaatc tccttcgacg gagcattcga tgatctggac tacaaagctc
    15061 tggtaaaaga cgaaatcgac tttgcaattc ttccgtctga gttccttccg ggaaatgcaa
    15121 aagatgagga agatgctgat gcagccgacg agaccgcaga taccaaagct gaggatcaga
    15181 aggacgataa agacgataag acaacggatg agaaagcaga cgaagacaag actccggaag
    15241 aacttctgaa agaggaaaat gagcgtctgt ccgacaccgc agagcgtctg gcaaccctga
    15301 ccattccgat gctggttgac cgttccgcag acgagaagac cgatcttgcg aaagcagagt
    15361 ggctgaaagt ctacggcgtg atcttcggat gcgaggatca ggcaaacgag ctgttccagc
    15421 agatggtaaa ggcagaagag aataaataag tacaataatt tgcagcaaaa aatgagaaag
    15481 ggctgctgcc gtatggcagc ggctttttct ttaagaaagg aatatcaaat gacaggaaaa
    15541 aacgaaagaa agctgaaaaa atgggccgca ctttttcttg cagcagcgat gtcactgacg
    15601 gcctgcggca gcaccgcatc aacgggaagc agcacatcgt ccgcgtcggg cacttcggca
    15661 gtttctgaga gcagcacatc cacagcatcc gcagcagacg tttccgaaag ccagacggag
    15721 agcacatccg tctcggaaag tacggctgaa agtgaaagca cctccgaagc cactgctgag
    15781 agcagcacct ccgggagtga taccgcatcc gcggatggtt tcgcaacgac agacgatgtc
    15841 agcgacgcac cggacatcac cggaatcacc atcgagagca agatggtcct gaattacgcc
    15901 gagtgcttta acgtatacta ctgcacagac ggctataaac tgatcgatgt caaagacggc
    15961 gcgcagtatc tgctcgtccc ggacggaaaa gaggcgccgg atgacctcga ttccgacgta
    16021 attgtaatcc atcagccgct ggaacgcatc tacatggcag caacctctcc gatggcgctg
    16081 ttcgatgcca tcgactccct cgatacgatt cgtctctccg gtgaaaccgc agacaactgg
    16141 tacgtacagg atgccgttga cgcgatgaat gccggaacga tgatcttcgc cggaaaatac
    16201 agtgagccgg attacgaact tctggtcagt gagaactgtg atctggccat tgaatccacg
    16261 atgatcctcc attccccgaa ggtacaggag atgatcgaga tgctcgatat tccggtcttc
    16321 atcgaccgtt ccagctacga atcccagcct cttggcagaa ccgaatggat caaactctac
    16381 ggtgccatgc tggacaaaga ggaggaggca gccgacttct ttgcaagtca ggcaagtgtt
    16441 gtggaaaatc tgaaggattt ccagaatacc gaaaaaacgg tcgctttctt ctatattaat
    16501 acgagcggag ctgccgttgt gcggaacccg gaggactata tttccaacat gatcgagctt
    16561 ggcggtgcgc gcaacgcctt ccgtgatctc caggacgaca gcggaaaaac atccgtgccg
    16621 atcacgatgg agcagttcta cgatacggcc gaagatgcgg actacctgat ctacaacgca
    16681 agcatcgatt ccaccgtcaa aaccctggac gacctgctgg caaaggattc catgtttgcc
    16741 gattacaaag cagtcaaaga gggcaacgtc tggacaaccg agaaatcgat gtatcaggca
    16801 accgataaaa ttgcacagtt taccaacgac attaaccttc tggtaacggg cggcgatccg
    16861 gatcagatgg tattcttaag aaaattatcc tgatggagga acgtatgtca gagaaaaaaa
    16921 atatgacagg aaaagagaac agtttccata tggaaaactt ccggcgccgt tcgcggtatg
    16981 ccgctgtgtt cctggtgctg attctggcgt tcgccgccat caccgtgctg aatatcaatt
    17041 ccggaaacgt aaatatttcg attccggaaa ttttccggat tctgctgcgg cggaccggag
    17101 aggggaaaga gtacaatatt atctggaaaa tccgccttcc gcgtattctg atggcggcaa
    17161 tcctgggcgg tgctctgtcc ctgtcgggct tccttttaca gactttcttt gaaaacccga
    17221 ttgccggtcc gtttgttctt ggaatttcct cgggagccaa aatggtcgtg gcgctgacca
    17281 tgatctttta tctggagtgg gcgcgcaata tcacatctta cacgatgatt gttgcagcat
    17341 tcttaggatc ccttctctcc gtcggcttca ttctgctgat gtcccgacgg atcaaaaata
    17401 tggcaggact tctcgtcggc ggtattatga tcggttacat ctgctcggcg gtcacggatt
    17461 ttgttgtcac cttcgcggag gattccgata tcgtcaacct ccacggctgg tcacagggaa
    17521 gcttctccgg catgagctgg agcaacgtag cgatctctgc ggctgtgatc ggcatcacct
    17581 ttgtcatcac gttcctgatg gcaaaaccga tcggtgcata ccagctcggt gaggcgtatg
    17641 cgcagagcat gggtgtcaac atcaaagtat tccgcatcgc gctgattctg ctttcgagta
    17701 ttctctccgc atgtgtcacc gcatttgccg gtccgatttc gtttgtcggc attgccgtcc
    17761 cgttcctcgt caaacagagc ctcggcacat cgcgtccgct cgtcgtaatc ccgggaacgt
    17821 tcctcggcgg agccgtcttc tgtatgatgt gcgacctgat cgcgcgtatg gcgtttgcac
    17881 cgctggagct gtccatcagt acggtcacat cgatctttgg tgcgccggtc gtcattttca
    17941 tgatgctgcg ccggaaaagg gggtaagaga atatgaatga atattatttt catctggacc
    18001 acctgacggt cggctatgat aagaagccgc tgatcaaaga tatctgcatc ggcatcgaaa
    18061 aaggcgagat cgttacgctg atcggaccga acggttccgg taaatcaacg attctgaaaa
    18121 gcatcacccg ccagttaaag ctggtcggcg gaaatgtgga gtttgacggg aaaaacctgc
    18181 atgagctttc gttccgcgag ttgtccacga agatggccgt tgtgctgacg gagcgcatga
    18241 agccggagct gatgacctgc cacgatatcg tggcaacggg ccgctatccg tacaccggac
    18301 ggctcggcat gctgacccgc gaggatgagg agaaggtcga gaaggcgatg cgcgccgttc
    18361 atgccgaaga gctcggcggc cgtgatttca acgctatcag cgacggccag cgccagagag
    18421 tgcttcttgc gcgtgcgatc tgtcaggagc cggaggtcat catcctcgac gagccgactt
    18481 ctttcctcga tatccgccac aagctggaac tgctcgcgat tctgcgccgc atggcaaagg
    18541 agaagggcat caccgtcatc atgtcgctgc atgagatcga tctggcacag aaaatttcag
    18601 ataagatcat ctgcgtcaag ggcgatgcta tttcccattt cggcgcaccg gagacaattt
    18661 tccgcgagga tatcatccgc gaactgtacg agattgacaa tggttcgttt gacccgtgct
    18721 tcggaagcat cgagctgcca cggccggagg gaacaccgcg cgtctttgtc ctcgcaggcg
    18781 gcggaacggg aatcccggtg ttccggaaac tgcagaagga aaatgtcccg tttgcggcag
    18841 gcgtcctgta tacgaacgat atcgattacc agctcgcccg cattctggcg atggagacgg
    18901 taaccgaagc accgttccag gagatcagcg acgaggcatt tgcccgcgcc tgcgagctga
    18961 tgaagagctg tgaacgtgtc atcgacaccg gcgtcccggt gggcatgtgc aaccgccgga
    19021 tcgaagagct gagggcagaa gcaaagcggc ttggaaagct ggcagagtaa aacaaagaaa
    19081 aatccaatca aaatctcctg tctgggaacg aaacaattgt tttccgaaca ggagattttc
    19141 tttttgcagc cattgtaaaa acagcaaaag aacacgaaaa atgtaatatt aaattgtacg
    19201 aaaaattcac acaattcaag tcaaaaacca acagagattg acacttaaaa agtcaagtgc
    19261 tttgtaaaat cttttggcga aataacggtt ttctctgtta tgaccaaaac acgcgttctg
    19321 atttcgcaag atttaaacac atgctttcca caccgttttt cgtggataat gtggataact
    19381 cggtgtataa cttgttttta ggcatgtttt cggtggttaa ctatgtggat aacttgtgga
    19441 cggg
//
LOCUS       DAOMUF010000017        19136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_502_length_19136_cov_4.110214, whole genome shotgun sequence.
ACCESSION   DAOMUF010000017 DAOMUF010000000
VERSION     DAOMUF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 19136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19136
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_502_length_19136_cov_4.110214"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..469)
                     /gene="rpsT"
                     /locus_tag="PLN77_02230"
     CDS             complement(206..469)
                     /gene="rpsT"
                     /locus_tag="PLN77_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRM43133.1"
                     /translation="MANIKSAKKRILVNQTKADRNKAIRSAVKTSMKKVDAAVAANDK
                     EAAAAALVNAISTIDKAASKGVYHKNNAARKVSNLSKAVNSIA"
     gene            651..1538
                     /gene="gpr"
                     /locus_tag="PLN77_02235"
     CDS             651..1538
                     /gene="gpr"
                     /locus_tag="PLN77_02235"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HRM43134.1"
                     /translation="MIGKYRMRTDLAMENQEKFERDHVEISGVEIEKKKRKAEIQTTI
                     VKITSEQGAKMMGKPKGTYVTIEAPLLLTADEEESRKAAEEFSHCLMEMVPEACGSVL
                     VAGLGNRGITPDALGPETVEQLNVTRHLVRTYGKKGREVSALAPGVMAQTGMEAVEIL
                     RGTAEQTHPDVILAVDALAACSIHRLNCTIQLADTGIWPGSGVGNHRNALNQETLGVP
                     VIGIGVPTVVGAGTIVHDAVAGVLESLEESEMDEFLGEVISPALESLYVTTKEIDEMV
                     RRLAGVLAAGMNQAFSGEL"
     gene            1562..2968
                     /locus_tag="PLN77_02240"
     CDS             1562..2968
                     /locus_tag="PLN77_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HRM43135.1"
                     /translation="MWNARRNGRWFGWGCLFLSCSILLFRSAAVLRKNHFSEGKVLLQ
                     RAAAQAEQLSVDVMMPLLFYDGKEDGRGWMENLLAAVRDQIPALREGSTAENTLSAGT
                     EAAETDTAKQDSNPGPGAERGQTESKEQGTEEDQKDGTQKDETQKDGMEITEPEQTET
                     GITEPAAGESASTAEPEAPQSVPEETIETGGAPREILYPEADTIQETISPDLLADQQA
                     LLNRFFVVDPNTSVLPGEIDGSTLLGKDLTLPENAEGPQILIYHTHSQETFADSRPGE
                     RADTVIGLGDDLQELLEKQYGYEVLHITEAFDMKEGCLERSRAYNYAEPVIAAALEEH
                     PSIQVVIDLHRDGVNGSKRLVTEINGKPTAQIMFFNGLSRTKESGELTSLPNPYQAEN
                     LAFSFQMEYEAEQYYPGFSRCIYLKGYRYNLHLRPRSVLLEVGAQTNTVEEVKNAMGP
                     FSDILHKVLSGKSPASGG"
     gene            3080..4894
                     /gene="lepA"
                     /locus_tag="PLN77_02245"
     CDS             3080..4894
                     /gene="lepA"
                     /locus_tag="PLN77_02245"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRM43136.1"
                     /translation="MSGIDQSKIRNFCIIAHIDHGKSTLADRIIQKTGLLTEREMQDQ
                     VLDNMDLERERGITIKAQAVRTVYKAADGEEYILNMIDTPGHVDFNYEVSRSLAACDG
                     ALLVVDASQGIEAQTLANVYLALDHDLDVFPVINKVDLPSAEPDRVIAEIEDVIGLEA
                     QDAPRISAKTGLNVDEVLEQIVQKIPAPEGDPTEPLKALIFDSVYDAYRGVIISCRVM
                     EGSVKKGTRIRMMATGAVEEVVEVGYFGAGRLIPCDELSAGMVGYITASIKNVRDTRV
                     GDTVTDADRPCAEALPGYKKVNPMVYCGMYPADGAQYGDLRDALEKLQLNDASLFYEP
                     ETSAALGFGFRCGFLGLLHLEIIQERLEREYNLDLVTTAPSVIYKVHKTDGTVIDLTN
                     PSNLPDPSEIDYMEEPLVKAEIMVTTDYIGPIMELCQQRRGQYQGMEYMETTRAMLHY
                     HLPLNEIIYDFFDALKSRSKGYASFDYELLGYERSELVKLDILVNKEEVDALSFIVFE
                     GSAYERGRKMCEKLKEEIPRQLFEIPIQAAVGSKIIARETVRAMRKDVLAKCYGGDIS
                     RKKKLLEKQKEGKKRMRQVGNVEIPQKAFMSVLKLDEN"
     gene            4983..6116
                     /gene="hemW"
                     /locus_tag="PLN77_02250"
     CDS             4983..6116
                     /gene="hemW"
                     /locus_tag="PLN77_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRM43137.1"
                     /translation="MNKKRKPLELYVHIPFCIRKCAYCDFVSGPGTKAMQKEYEEALL
                     AEIDAAEETAEREVISVFFGGGTPSAVDAGMLARVMEKLRSKYVFSEDAEITLEANPG
                     TLDAEKLKCYRKSGFNRISIGCQSVHDEELKRLGRIHTFAEFQESFVLARDAGFANIN
                     VDLMSGLPEQSEEKWEESLRTIAELSPEHISAYSLIVEPGTPFAEQKLDLPDEDTERE
                     MYARTAEILAEYGFFQYEISNYAKPGFACRHNIGYWKRTDYLGFGPSAASLFGNRRWT
                     NTADRSLYLKACGALEKIREDEEILSRQDAMEEFMFLGLRMTQGISTAEFEEKFGKEI
                     HAVYGGVLKKYEAMHLLQEHSGRLALTRDGISVSNVILADFLL"
     gene            6241..9069
                     /gene="uvrA"
                     /locus_tag="PLN77_02255"
     CDS             6241..9069
                     /gene="uvrA"
                     /locus_tag="PLN77_02255"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRM43138.1"
                     /translation="MAEKKEFIRIRGARENNLKALNVDIPRNQFVVFTGLSGSGKSSL
                     AFDTIYAEGQRRYMESLSSYARQFLGQMEKPNVDSIEGLPPAISIDQKSTNRNPRSTV
                     GTVTEIYDYFRLLYARIGIPHCPKCGKVISRQSVDQMVDQIMALPERTKIQLLAPVVR
                     GRKGRHEKVLEQAKRSGYVRVMIDGSQYELSEEITLDKNLKHNISIIVDRLVVKPDIE
                     KRLTDSVENVLDLADGLLVVDTMDGNQLNFSQSFSCPDCGISMEEIEPRSFSFNNPFG
                     ACPSCAGLGYKMEFDEDLMIPDKTVSIADGAITVLGWQSCTDKSSYTRAVLDALAEEY
                     DFSLSTPFCEYPKKIHDILIYGTNGKSVKVHYRGQRGVGVYDVAFEGLIKNVERRYRE
                     TGSDSTKQEYETFMRVTPCQACHGQRLKPTSLGVTVGDKNIFEVTSLSIDNLQKFMQN
                     LELSEQQLLIGKQILKEIRARVSFLADVGLNYLTLARGTASLSGGEAQRIRLATQIGS
                     GLVGVAYILDEPSIGLHQRDNDKLLATLKHLRDLGNSLIVVEHDEDTMRAADCVVDIG
                     PGAGEHGGELVAIGTAEDLMKNPNSITGKYLSGELKIPVPSERRKPTGWLKVVGAKEH
                     NLKNINVNFPLGVMTCVTGVSGSGKSSLVNEILYKRLARDLNRARTIPGAHKDIIGME
                     QLDKVIDIDQSPIGRTPRSNPATYTGLFDQIRDLFAATQDAKARGYKKGRFSFNVKGG
                     RCEACGGDGILKIEMHFLPDVYVPCEVCHGKRYNRETLEVKYKGKSIYDVLNMTVEEA
                     LEFFRPVPSIFRKLQTLYDVGLSYIRLGQPSTTLSGGEAQRIKLAAELSRRGTGKTIY
                     ILDEPTTGLHFADVHKLVEILHRLSEGGNTVVVIEHNLDVIKTADYIIDIGPEGGDGG
                     GTVIACGTPEEVAKSPVSYTGKYVEKYL"
     gene            9238..10236
                     /locus_tag="PLN77_02260"
     CDS             9238..10236
                     /locus_tag="PLN77_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRM43139.1"
                     /translation="MAATTDIGIDLGTSSILVYAKGKGIVLKEPSVVAYDKDADKVRA
                     IGEEARQMIGRTPGNIMAVRPMRQGVITDFLITERMLRYFIQKAMGRRAFRKPRISIC
                     VPSGVTEVERKAVEEATYQAGAREVLIVPEPVAAAIGSGIDITKPCGNMIVDIGGGTT
                     DIAVISLAGTVVSNSVKISSETFDQDIIRYVRKTYNVFIGEQTAEAVKIRIGSAYPRA
                     EEKTMEIKGRNVITGLPATITVSSDEIRGALAQTTNQIADAICAVLEKTPPELASDVS
                     DRGIILTGGGSLLDGMDELIQERTGINTVTAENPASVVALGTGQYREVMNEFESQF"
     gene            10243..12498
                     /locus_tag="PLN77_02265"
     CDS             10243..12498
                     /locus_tag="PLN77_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRM43140.1"
                     /translation="MSEKEKVEGYVEHIIFRNEENGYTVLNLSMKGRELTCVGTLPMI
                     GEGELIEASGDYIEHAAYGKQFRIESYETKVPQDSVALERYLGSGAIKGVGAALAARI
                     VRRFGDDTLRIIEEEPERLAEVKGISERKAREIAQQVAEKAEMQNAMIFLSGYGIALN
                     LGAKIYQQYGDNVYRILKENPYKMAEDIAGVGFRTADEIAGKIGIAVDSEFRIKSGLS
                     YVLNQATAEGHVYLPEPVLLRRGQELLGVEPERMQKSLQDMAIDKKAVIRELPAEREG
                     DEPLRIVYASQYYYLELSTARMLHELNITCEEDREAIEKRIGKIEKKYQIELDEMQKT
                     AVVEAAYSGLMILTGGPGTGKTTTINAMIHYFEAEGLDIFLAAPTGRAAKRMTEATGC
                     EACTIHRLLELTGNVDDSSANVHFERNADNPLDADVIIIDEMSMVDIHLMHALLSAIV
                     PGTRLILVGDQNQLPSVGPGSVLRDLIRSECFPIVCLTHIFRQASQSDIVVNAHKIHN
                     GEEVILDNKSKDFFFLKRYDVNVIWKVLVTLVSQKLPRYVDARPQDIQILTPMRKGAL
                     GVENLNQILQKYLNPPAPDKAERENGGNILREGDKVMQIRNNYQMEWEIRGINGIVAE
                     RGMGVFNGDLGMIRSINPYTEVITVEFEEGKFADYTFKQADELELAYATTIHKAQGSE
                     YPAVVIPLLGGPRMLMTRNLLYTAVTRARKCVTIVGSDVTFQAMIGNHIEANRYTTLA
                     RRIREMQEENA"
     gene            12565..13284
                     /locus_tag="PLN77_02270"
     CDS             12565..13284
                     /locus_tag="PLN77_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRM43141.1"
                     /translation="MKEKNKTGNWLLNAVYPRRCPICDGLLSREELYLCRNCVEKIHP
                     ILGARCKKCGKPVGSEEEFCRDCSRTRHSFDRGFAPYGYHGELEASLMRFKYHERQEY
                     AGFYAHAAFFYVGKQIQRRNPEVLVPVPLHPARQRKRGYNQAEVFAEKLAEEMELPVN
                     ARLIRRVKKTKRQKELGPAERRKNLEGAFALAKPGQMKYCRILLVDDIYTTGSTVDAI
                     ASLLKVNGAEQVDFVCAAIVK"
     gene            13374..13805
                     /locus_tag="PLN77_02275"
     CDS             13374..13805
                     /locus_tag="PLN77_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43142.1"
                     /translation="MLDEKRIRQMTKLAFFEKKEEKNALRIGRYFRSDYIGKELLKNF
                     FLASIGYILVLAVAVLYHIEWLVDEIDTIDLQFAAGCLIASYLCFLAVYSIAVYVIST
                     IRYQGAQKKLKWYGKELDDLQNMYEEQEKSGKKSGRRRKET"
     gene            13802..14980
                     /locus_tag="PLN77_02280"
     CDS             13802..14980
                     /locus_tag="PLN77_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43143.1"
                     /translation="MTVLIELKEKLKEFYGSCSGIVLPIVKFLLALAVFRGINSNLGY
                     LKMLNSMYVVLILSLICAILPLNGMVFLAMLVIIGHCFALHMVIAGLAALVLLVLWFL
                     YLRFVPKDAPALLLTPLAFWLHVPSAVPVAYGLAGTPLSAFSAACGVVVYYMCDMIHG
                     KMEPLLHAAEAPEITAVVQEFFNGLFRNEEMLLVLIACALTVLLVNAIRHSSADYAWQ
                     ISIVAGSVAYAVIMIAGSLALDVQIALPMVLIGAAAGCLVGFVLEFFLFGADYTRTEY
                     LEYDDDDYYYYVKAVPKFNVTKAQRRVTYIRNEKKKEPDLTDEELDALANLGHTEESG
                     NSRDLDEIEEKEEIEEINETEEINETEEIQEEEIPPLDEGDYQDVDYKSMLEDTLKDL
                     "
     gene            15046..15924
                     /gene="cdaA"
                     /locus_tag="PLN77_02285"
     CDS             15046..15924
                     /gene="cdaA"
                     /locus_tag="PLN77_02285"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRM43144.1"
                     /translation="MWNWIVTRTADYGLPKQIELIDIVQILLIAFFVYHLIRWLQNTK
                     AYTLMKGIFLIGVFVIIANVLKMQTILWLFGNIGGAAITAVVIIFQPELRRVVEQIGE
                     KNPLSAISFASGQEENERFSDKTINELVRASFEMGEVKTGALIVIEKTITLEEYEKTG
                     IPIDGVLTSQLLINIFEHNTPLHDGAVIVRNNRVTAATCYLPLSDNMDLNKNLGTRHR
                     AGVGISEVTDSFTIIVSEETGNVSYASGGELHTAVTPSDLREQLHKIQKLAKKPEEKK
                     GWHIRGMRQKVEGEKK"
     gene            15921..17237
                     /locus_tag="PLN77_02290"
     CDS             15921..17237
                     /locus_tag="PLN77_02290"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRM43145.1"
                     /translation="MKKKILKKLLNNLPLKILSILIAIVIWYVVVSVNDPIVKERFDV
                     PVQVTNEAYIAAGKKTYQIEEEYQTVTVTVTDNNSVVSRLKASDITVTADLTQIVTMD
                     TNPVYVPIKAACSMVKQEKLSTVTATIPVEIEDVDSEKFPITIDAGNTKPAKDYEVGT
                     MTSDPESITISGPTSLINKISSVVAKVDVTNMRNSGTVTADLMIIDKNQDEMSESQME
                     FLNFDSGSPQVDVDIELWRRVSGIKLSALYSGTPADGYQIKNIYTTPEEITVAGSEEA
                     LAKLADEGNTIEIPEDYTSVAGQRSDVETTVDLSDVLADVTDLKVSSSSSASVTVHVT
                     VMPNESREFELDVDQIETSNLQSTYTVLYDQTQLAIRIKASDKNLAKLDTSQINASID
                     LNGMGVGDYEVPVKITLPDGYELVDSVKTTIHIKEKAVANKTTGTN"
     gene            17249..17521
                     /locus_tag="PLN77_02295"
     CDS             17249..17521
                     /locus_tag="PLN77_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM43146.1"
                     /translation="MVSGKVTVKNPTGLHLRPAGTLCKQAMQFKSSITFHYGDGNTAN
                     AKSVLSVLGACVKCGDEIELFCEGEDEEEALQFLVNSIRTGLGEEL"
     gene            17534..18634
                     /gene="glf"
                     /locus_tag="PLN77_02300"
     CDS             17534..18634
                     /gene="glf"
                     /locus_tag="PLN77_02300"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HRM43147.1"
                     /translation="MKKYDYVLVGAGLYSGVIAYLAGQAGKSCLVLERRDHTGGNIYC
                     EEIEGIHVHKYGAHIFHTSNKEVWQFVNSLAEFNRYTNSPIANYKGEIYNMPFNMNTF
                     SKMWGIRTPEEAKTIIEEQRKAVPGEPKNLEEQAIKLVGKDIYEKLVKGYTEKQWGRD
                     CSELPSFIIKRLPVRFTYDNNYFNDLYQGIPIGGYNVLTERLFEKADVQTGVDFLEDK
                     EKYLAMGETVIYTGAIDAFYDYALGKLEYRTVRFETEVLDTDNYQGVAVVNYTDRETP
                     YTRVIEHKHFEFGTQKKTVISREYSTDWKEGMEPYYPVNDARNQELYQKYAALAEKEE
                     KVIFGGRLGEYKYYDMDKVIEAALNQWEKMQR"
     gene            18763..>19136
                     /locus_tag="PLN77_02305"
     CDS             18763..>19136
                     /locus_tag="PLN77_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43148.1"
                     /translation="MRKEYDDEDEIPERRHSSGKKKKKFGWVNVLTILLVVVLVVLVG
                     VYAVINHYYSKSNYVSDDKITINKSTEVETESTGLTDEETDALQSEILNETQDIELPN
                     NNNVYNILLIGVDRRDKTWYGNS"
BASE COUNT         5281 a         4332 c         5419 g         4104 t
ORIGIN      
        1 ttcacgctct gcggtgctga attttttgca ttattttcgg gtcttgctat aaaaagaaca
       61 aggcgtaccg gtacaggaaa actggccgat acgccttgtt ctgtgtttat caaaagatct
      121 gtcgcggcgc actcttttgg taagcataaa aaaagaaccc agccacatga cggtatgact
      181 aagttctctt tgttttatat ctttattatg cgattgagtt tacagctttg ctcagattgg
      241 atacttttct ggcagcgttg tttttatgat aaacgccttt agaagcagcc ttgtcgatgg
      301 tagagatcgc atttactaat gcagcagcag cagcctcttt atcgtttgca gcaacagcag
      361 catctacttt tttcatagaa gtcttaactg cagaacggat tgctttgttg cggtctgcct
      421 ttgtctgatt taccaggatt cttttctttg cggatttaat gttagccaaa ctgtacacct
      481 ccaatatttt taaaattaat gtgatttaag ggatgttatt caatggaaca gacacgggcg
      541 ctccactgaa acatgcttta ttatcctata atatttcgca tggtttgtca atacatattt
      601 ttgaaaaaat tgcagaaact acaacaaaaa tacaggagaa ggagaaaagt atgataggaa
      661 agtataggat gcggacagac ctggcgatgg agaatcagga aaaatttgag agggaccatg
      721 tggagatctc cggggtcgag attgagaaga agaaaagaaa ggcggaaatt cagacaacca
      781 ttgtgaaaat cacatcggag cagggtgcaa aaatgatggg aaagccgaaa gggacgtatg
      841 tgacgatcga agcgccgctg cttttgacgg cagacgagga ggagagccgg aaagcggcgg
      901 aggagttttc ccactgtctg atggaaatgg tgccggaagc gtgcggatcc gttcttgtgg
      961 ccggccttgg caaccgcggc atcacgccgg atgcgctcgg gccggagacg gtggagcagc
     1021 ttaacgttac gcgccatctg gtgcgcacct acggaaagaa gggccgggaa gtgagtgccc
     1081 ttgctcccgg cgttatggca cagacgggca tggaggcagt ggaaatcctg cgcggaacgg
     1141 cagaacagac ccatccagat gtgattctcg ccgtggatgc ccttgccgca tgcagtattc
     1201 accggctgaa ctgtacgatc cagcttgccg acaccggaat ctggccgggc tcgggcgtcg
     1261 gaaaccaccg caatgcctta aatcaggaga cgctcggcgt gccggtcatc ggaatcggtg
     1321 tgccgaccgt tgtaggggca ggaacgatcg tccacgatgc cgtcgcaggc gttctggaat
     1381 cgctggagga gtccgagatg gatgaatttc tgggagaagt catttcaccg gcgctcgaaa
     1441 gcctttacgt gacgacaaaa gagatcgacg agatggtccg ccgcctggcc ggggttcttg
     1501 cggcaggtat gaatcaggca ttttccgggg aactgtaaca tatcatgaat atggtgataa
     1561 tatgtggaat gccaggcgaa atggacgatg gttcggatgg ggatgtcttt tcctgtcctg
     1621 cagtatactg ctgttccgca gtgcagcggt tctccggaaa aatcatttct cagaggggaa
     1681 agttctgctg cagcgggcgg cagcacaggc ggagcagtta tccgtggatg tgatgatgcc
     1741 gcttcttttt tatgacggaa aagaggacgg aaggggatgg atggaaaatc tcctcgcggc
     1801 agtgcgggat cagataccgg cgcttcggga gggaagcacg gcagaaaata cgctgtctgc
     1861 cgggacagaa gcagcggaaa cggatacggc gaagcaggat tcgaatccgg gaccgggagc
     1921 agaaagaggg cagacagaat cgaaggagca gggaactgag gaagatcaaa aggatggaac
     1981 tcaaaaagat gaaactcaaa aagatggaat ggaaatcacg gagccggagc agacggaaac
     2041 aggaatcaca gaaccggcag ccggagagtc ggcatcaacg gcagaacccg aagcgccgca
     2101 gtctgtcccg gaggagacca tcgaaaccgg cggcgcaccg cgtgagattc tctacccgga
     2161 ggccgacacg attcaggaga caatttcccc tgaccttctt gctgaccagc aggcgctcct
     2221 gaaccgcttc ttcgttgtcg atcccaacac ttcggtcctg ccgggcgaga tcgacggcag
     2281 caccctgctc ggaaaagacc tgaccctgcc ggaaaatgcc gaaggcccgc agattctgat
     2341 ctatcatacc cattcgcagg aaaccttcgc cgattcccgt ccgggagaaa gagcggacac
     2401 ggtaatcggc ctcggtgatg atctgcagga gcttctggaa aagcagtacg gatacgaagt
     2461 gcttcatatt acagaggcat ttgacatgaa agaaggctgt ctcgagcgga gccgcgccta
     2521 caactacgcg gagccggtca tcgcagcggc gctggaagaa cacccgtcca ttcaggtcgt
     2581 gatcgacctg caccgggacg gggtcaatgg ttcaaaacgt cttgtgacag aaatcaacgg
     2641 aaagccgacc gcgcagatca tgtttttcaa cggcctttcc cgcacgaaag aaagcggcga
     2701 actgacaagc ctgcccaatc cgtatcaggc ggaaaacctt gcattttcct ttcagatgga
     2761 gtatgaggca gaacagtatt atcccggctt ttcacgctgt atctatttaa aaggctaccg
     2821 ctacaacctc catctccgcc cgagatctgt tcttctcgag gttggtgcgc agacgaatac
     2881 ggtcgaggag gtgaaaaatg caatggggcc gttttcggat atcctgcata aggttctgtc
     2941 gggcaaaagt ccggcatcag gcggataaat ttatcttgct tttttgagtg gaatttcgta
     3001 tcatacaaaa tagacagatt gcgggagtac gaaatatgga gcaatctggt atcgtaggga
     3061 aaaagcagga ggaaaaaact tgtcaggaat agatcagagt aaaattcgta atttttgtat
     3121 tattgcccat atcgaccatg gaaaatcaac ccttgcggac cgtattattc agaaaacagg
     3181 ccttttgacc gagcgtgaga tgcaggatca ggtgctggac aatatggatc tggagcgtga
     3241 gcgcggcatc acgatcaagg cacaggcagt ccgtaccgta tacaaagcgg ccgacggcga
     3301 ggagtacatt ttaaacatga ttgacacccc gggccatgtt gacttcaact atgaggtttc
     3361 ccgaagcctt gccgcatgcg acggcgcgct gctcgttgtc gatgcatcgc agggtatcga
     3421 ggcgcagacc cttgccaacg tctacctcgc ccttgaccat gacctggatg tgttcccggt
     3481 catcaacaaa gtagaccttc caagcgccga gccggaccgt gtaattgcag agatcgagga
     3541 tgtcatcgga ctggaagcgc aggatgctcc gcggatttcc gcaaaaaccg gcctgaacgt
     3601 ggacgaggtg ctcgagcaga tcgtacagaa aattccggca ccggaaggtg atccgacgga
     3661 accgttaaaa gcactgattt tcgactccgt ctacgatgca taccgcggcg tcatcatttc
     3721 ctgccgtgtg atggaaggaa gcgtgaaaaa aggaacgaga atccgcatga tggcgacagg
     3781 cgctgtcgag gaagttgtcg aggtcggcta cttcggcgca ggccgtctga tcccgtgcga
     3841 cgagctctcc gccggaatgg taggctatat caccgccagc atcaaaaacg tgcgcgacac
     3901 ccgcgtgggt gataccgtga ccgatgccga ccgtccgtgt gcagaagcac tgccgggcta
     3961 caaaaaagtc aacccgatgg tatactgcgg catgtacccg gccgacggcg cacagtacgg
     4021 cgatctgcgc gacgcgctgg aaaaactgca gttaaacgat gcatccctct tctacgagcc
     4081 ggagacatcg gcagcactgg gctttggctt ccgctgcggt ttccttggac tgcttcacct
     4141 ggaaattatt caggagcgcc tggagcgtga gtacaatctg gatctcgtga cgaccgcacc
     4201 gagcgttatc tataaggttc acaaaaccga cggaaccgtc attgacctga ccaacccgtc
     4261 gaatctgccg gatccgtccg agattgatta tatggaagag ccgctggtaa aagcagagat
     4321 tatggtgacg accgactaca tcggaccgat catggagctc tgccagcaga gacgaggcca
     4381 gtatcaggga atggaataca tggagacgac gcgtgcgatg cttcattacc atctcccgct
     4441 gaatgagatt atctacgatt tctttgatgc cttaaaatcc cgctccaaag gttacgcgtc
     4501 attcgactac gagctgctcg gctatgagag aagcgagctt gtaaaactcg atatcctcgt
     4561 caataaagaa gaagtggatg cgctgtcctt catcgtattc gagggatctg cctacgagcg
     4621 cggaagaaag atgtgcgaga agctcaaaga agagattccg cggcagctct tcgaaatccc
     4681 gatccaggcg gctgtcggat cgaagattat cgccagagaa accgtccgcg cgatgcgcaa
     4741 agacgtcctc gccaagtgct acggcggcga tatttcccga aagaagaagc tgctggagaa
     4801 gcagaaggaa ggtaagaagc ggatgcgcca ggtaggaaac gtggagattc cgcagaaagc
     4861 ctttatgagc gtgctgaaac tggatgaaaa ttaagagata aaaatgagac cggcctgtcg
     4921 gcgccgcctg ctgttgcggg agcgcagggg ccggtctttc gatcgtacgg aggaaaagca
     4981 gaatgaataa aaaaagaaaa ccactggaac tctatgtgca tatcccgttc tgcatccgga
     5041 aatgtgccta ctgtgatttt gtctcgggac cgggcacgaa agcaatgcag aaggagtatg
     5101 aagaggctct gctggcagaa atcgatgcag cggaagagac ggcagaaagg gaagtgattt
     5161 ccgtcttttt cggcggcgga acaccatccg cagtggatgc cggaatgctc gcccgtgtga
     5221 tggaaaaact gcggagcaag tacgtttttt cagaggatgc tgagattacg ctggaggcca
     5281 atccgggaac gctggatgcg gaaaaactga aatgttacag gaagagcggc ttcaaccgga
     5341 tcagtatcgg ctgtcagtcg gttcacgatg aggagctgaa acggctggga agaatccaca
     5401 cgtttgcgga atttcaggag agttttgtgc tggcacggga tgccgggttt gcgaatatca
     5461 acgtggatct gatgtcaggt ctgccggagc agagtgaaga gaaatgggaa gagagtcttc
     5521 ggacgatcgc ggagctttct ccggagcaca tttccgccta cagcctgatc gtggagccgg
     5581 gaaccccgtt tgcggagcag aaactggatc tgccggatga ggacacagaa cgcgaaatgt
     5641 atgccagaac ggcggaaatc ctcgcagaat acggcttttt ccagtatgag atttccaatt
     5701 atgcaaagcc gggcttcgca tgccgccaca atatcggcta ctggaaaagg acggattatc
     5761 tggggttcgg gccttccgcg gcatcgttat tcggaaaccg ccggtggacg aacacggcag
     5821 atcgatcttt gtatctgaaa gcgtgcggag cactggaaaa gatccgtgag gatgaggaaa
     5881 ttctttcccg gcaggatgcc atggaagaat ttatgtttct cggccttcga atgacacaag
     5941 ggatctcgac ggcggagttt gaggagaagt ttggaaagga gatccatgcc gtatacggcg
     6001 gggttctgaa gaaatacgaa gcgatgcatc ttttacagga acacagcggg cgcctggctc
     6061 tgacacgcga tgggatttcc gtcagcaatg tgattctggc agattttctg ctgtaaaaag
     6121 atgataaaga aatagaacaa atgtttgcat aacgcacggc ggtgtgctat aatgggagaa
     6181 aacgagacaa gattcctgtc cctgtccgga cagggattcc aattaataag aggaacagtt
     6241 atggcagaga aaaaagagtt tatcagaata cggggagcca gggagaacaa cctgaaagca
     6301 ctgaatgtcg atatccccag aaaccagttt gttgtattta cgggcctcag cggttcagga
     6361 aagagttcgc ttgcgtttga cacgatctat gcggagggcc agcggcggta catggagagc
     6421 ctttcctcct acgcgcgcca gttcttagga cagatggaaa aaccgaatgt ggacagcatc
     6481 gaaggcctgc cgcctgctat ttcgatcgat cagaaatcga cgaaccggaa cccgcgttcc
     6541 accgtcggaa ccgtgacgga gatttacgat tacttccgtc ttctgtacgc gagaatcggt
     6601 attccgcact gcccgaagtg cggaaaagtc atcagccgcc agtcggtgga ccagatggtc
     6661 gatcagatca tggcactccc ggagcggacg aaaatccagc ttctcgcgcc ggttgtgcgc
     6721 ggccgcaagg gaagacacga aaaggtgctc gaacaggcaa aacgcagcgg ctatgtccgc
     6781 gtgatgatcg acggcagcca gtatgagctg tccgaggaga ttacgctgga taagaacctg
     6841 aaacacaaca tttccattat tgtggaccgt cttgtagtca aaccggacat tgagaaacgt
     6901 ctgacggatt ccgtcgagaa tgtactggat ctggcggacg gccttctggt ggtcgacacg
     6961 atggacggaa atcagctcaa cttcagccag agcttttcct gcccggactg cggcatcagc
     7021 atggaggaga ttgagccgag aagcttttcc ttcaacaacc cgttcggcgc ctgcccgagc
     7081 tgtgccggcc ttggctacaa gatggaattc gacgaggatc tgatgattcc ggataagacc
     7141 gtatccatcg cagacggagc catcaccgtg ctcggctggc agtcctgcac ggacaaatcg
     7201 agctatacga gagccgttct cgatgcgctg gcagaggagt acgatttttc cctttccact
     7261 ccgttctgtg aatatccgaa aaaaatccac gatatcctga tttacggaac gaacggaaaa
     7321 tccgtcaagg tgcattaccg cggtcagcgc ggcgtcggtg tgtacgatgt ggcttttgag
     7381 gggctgatca aaaatgtaga gcgccgctac cgtgagaccg gatcggattc tacaaaacag
     7441 gaatacgaaa ccttcatgcg tgtgaccccg tgccaggcgt gccatggcca gcgtctgaaa
     7501 ccgacgtcgc ttggtgtgac ggtcggagat aaaaatattt ttgaagtaac atccctttcc
     7561 atcgacaatc tgcagaagtt tatgcagaac ctcgaactct ccgaacagca gctgctgatc
     7621 ggaaagcaga ttttaaaaga gatccgtgcg cgtgtgagct tccttgcgga tgtcggatta
     7681 aactatctga ccctggcgcg cggaacggca tcgctgtccg gcggtgaggc gcagagaatc
     7741 cgtctggcaa cgcagatcgg ctccggcctt gtcggtgtcg cctatattct cgacgagccg
     7801 agcatcggtc ttcaccagag agacaacgac aaacttctcg cgacgctgaa acatctgcgt
     7861 gacctcggaa acagcctgat cgtcgtggag cacgacgagg atacgatgcg cgccgctgac
     7921 tgcgttgtcg atatcggccc gggagccggt gagcacggcg gagagctggt ggcgatcgga
     7981 acagcggaag atctgatgaa aaatccgaat tccatcaccg gaaaatactt aagcggagag
     8041 ctgaaaattc cggttccgtc cgagcgcaga aagccgacgg gctggctgaa ggtagtcgga
     8101 gcaaaagagc acaatctgaa aaatatcaac gtgaatttcc cgctcggtgt catgacctgc
     8161 gtgaccggag tctccggatc gggaaagagt tctcttgtca atgaaattct gtacaaacgg
     8221 ctggcaagag atctgaaccg tgcgcggacg attccgggtg cacacaaaga catcatcggt
     8281 atggaacagc tcgataaggt catcgacatc gaccagtccc cgatcggaag aacaccgcgt
     8341 tccaatccgg caacgtacac aggtctgttc gatcagatcc gcgacctgtt tgcggcaacg
     8401 caggatgcca aggcgcgcgg ctacaagaag ggaagattca gctttaatgt gaagggcggc
     8461 cgatgcgagg cctgcggcgg cgacggtatc ctgaagatcg aaatgcactt cctgccggat
     8521 gtctatgtgc cgtgtgaggt ctgccatgga aaacggtaca accgtgagac actggaagtc
     8581 aaatacaaag gaaagagcat ctacgatgtg ctgaatatga cggtcgagga ggcactggaa
     8641 ttcttcaggc cggtaccgtc gattttccgg aaattacaga ccctgtacga tgtcggcctt
     8701 tcctacatcc gcctcggcca gccgtccacc acgctgtccg gcggagaagc gcagcgtatc
     8761 aagctcgcag cagagctgag ccgccgcggc acgggaaaaa cgatctacat tctcgacgag
     8821 ccgacgacgg gacttcattt tgcggatgtc cacaaactgg tggagattct gcaccgcctg
     8881 tccgagggcg gaaataccgt cgttgttatc gaacacaatc tggatgtcat caagacggcg
     8941 gactatatta ttgatatcgg accggagggc ggagacggcg gcggaaccgt gattgcgtgc
     9001 ggaacgccgg aggaagtggc caagagcccg gtatcctaca ccggaaaata tgtcgaaaaa
     9061 tatctctaaa aaatcctttc cattttaaaa gggattgtat ataataagaa gcgggttggg
     9121 ttcagaacac attcgatcct tttgtgtttc gggtgttttg aacccgaact tattctatac
     9181 aaatttcaaa cagattaaaa taaagaaaaa taaaagagaa caggaaaggg gattactatg
     9241 gctgcaacga ccgatatcgg aattgatctg gggacgtcca gcatcctggt ctatgccaag
     9301 ggaaaaggca ttgtattaaa ggagccgtcc gtagttgcgt atgataaaga tgcagataaa
     9361 gtacgggcaa ttggagaaga agcgcgtcag atgattggac ggacaccggg caatattatg
     9421 gcggtccggc ccatgcggca gggcgtgatc acggactttc tgatcaccga gcgcatgctg
     9481 cgctacttta tccagaaggc aatgggaaga agagcattcc ggaaaccgag aatcagcatc
     9541 tgtgtgccgt ccggtgtgac ggaagtagag cggaaggctg tggaggaggc aacgtaccag
     9601 gcgggagccc gggaagtgct gatcgtgccg gagccggtgg cagcggcaat cggatccgga
     9661 attgacatta cgaaaccatg cggaaatatg atcgtggaca tcggcggagg cacgacggat
     9721 atcgctgtga tctcgcttgc gggaaccgtt gtttccaatt cggtcaagat ttccagtgag
     9781 acatttgacc aggatatcat ccgctatgta cggaaaacat acaacgtatt tatcggagaa
     9841 cagaccgcag aggcagtgaa aatccggatt ggttccgctt atccgcgggc cgaggagaaa
     9901 acgatggaga tcaagggaag aaacgtcatc accggacttc cggcgacgat tacggtttcc
     9961 tccgacgaaa tccgaggtgc ccttgcccag acgacaaacc agattgcgga cgcgatctgt
    10021 gccgtgctgg aaaaaacgcc gccggaactg gcatccgatg tttccgaccg cggcattatc
    10081 ctgaccggag gcggcagtct ccttgacgga atggacgagc tgatccagga gcggaccggc
    10141 atcaataccg tgacagcaga aaatccggcg agcgtcgttg cgcttggaac cggccagtac
    10201 cgcgaagtga tgaatgaatt tgaaagccaa ttttgataaa agatgagtga aaaagaaaaa
    10261 gtagaaggct acgtagaaca cattattttc cgaaatgagg agaacggcta taccgtcctg
    10321 aatctgagca tgaaggggcg cgagctgaca tgcgtcggca cgcttccgat gatcggagag
    10381 ggcgagctga tcgaggcttc cggagattat attgaacatg ctgcctatgg aaagcagttc
    10441 cgcattgaat cctatgagac gaaagttcca caggattctg ttgcattgga gcggtatctc
    10501 ggttccggtg cgatcaaagg agtcggtgcg gcgctggcgg caagaattgt gcgccgcttt
    10561 ggtgacgata cgcttcggat catcgaggaa gagccggagc gcctcgcgga ggtaaaggga
    10621 atcagcgagc ggaaagcgcg tgaaattgca cagcaggtgg cagaaaaagc cgaaatgcag
    10681 aatgcgatga ttttcctctc gggatatggc attgcgctga atctcggtgc aaagatttat
    10741 cagcagtacg gggacaatgt gtaccggatt ttaaaggaaa acccgtataa aatggcggaa
    10801 gatatcgcag gcgtgggatt tcggacggcg gatgagattg ccggaaaaat cgggattgcc
    10861 gtggacagcg aattccgcat caagagcggt ctttcgtatg tgctgaatca ggcgacggca
    10921 gaaggccatg tctatctgcc ggaaccggtg ctgcttcgga gagggcagga acttctcggc
    10981 gtagaaccgg agcggatgca gaaatctctg caggatatgg caatcgacaa aaaagcggtc
    11041 atccgcgagc tcccggccga acgggaaggg gatgagccgc tgcggattgt gtacgccagc
    11101 cagtattatt atctggaact gagtacggcg cgcatgctgc atgagctgaa tattacatgc
    11161 gaggaagacc gtgaggcaat cgaaaagcgg atcggaaaga tcgagaaaaa atatcagatc
    11221 gagctggatg agatgcagaa gacggcagtc gttgaggcgg catacagcgg tcttatgatc
    11281 ctgaccggag gtcccggaac cggaaaaacg acgaccatca atgcgatgat ccattatttt
    11341 gaggcagaag gcctcgacat tttcctggca gcgccgaccg gaagagcggc gaaacgaatg
    11401 acagaggcaa ctggctgtga ggcttgcacg atccatcgtc ttctggaact gacgggaaat
    11461 gtggatgact cctcggcaaa tgtgcatttt gagcggaacg cagataatcc gctcgacgcg
    11521 gatgtcatca tcattgatga gatgtcgatg gttgatatcc acctgatgca tgcccttctt
    11581 tccgcgattg tgccgggaac aaggctgatc ctggtgggag accagaacca gcttccaagt
    11641 gtcggcccgg gcagtgtcct ccgggatctg atccgttcgg aatgctttcc gattgtctgc
    11701 ctgacgcaca ttttccggca ggcatcccag agcgatattg ttgtcaatgc acataagatc
    11761 cataatgggg aagaggttat actggacaat aagagcaaag atttcttctt tttgaaacgg
    11821 tacgatgtca acgtgatctg gaaagtgctg gtgacgctgg tgtcccagaa actgccgcgt
    11881 tatgtggatg cccgcccgca ggatatccag attctgactc cgatgcggaa aggtgcactc
    11941 ggcgtggaaa atctgaatca gattctgcag aaatacctga acccgcccgc accggacaaa
    12001 gccgagcggg aaaatggagg taatatcctc cgcgagggcg acaaggtcat gcagatccgc
    12061 aacaattacc agatggagtg ggaaatccgc ggcatcaacg gaatcgtggc ggagcgcggt
    12121 atgggtgtat tcaacggaga cctcggcatg atccggagca tcaacccgta taccgaggtg
    12181 atcaccgtgg agtttgagga aggaaaattc gcagactata cgttcaaaca ggcggacgag
    12241 ctggagctcg cctacgccac aacgatacat aaagcgcagg gaagcgagta cccggcggtt
    12301 gtgattccgc tgctcggcgg cccgcgcatg ctgatgacgc ggaatcttct gtatacggcc
    12361 gtgacgagag ccagaaagtg cgtcaccatc gtcggaagtg atgtgacatt ccaggcaatg
    12421 atcggaaatc atatcgaggc gaaccggtat acaacgctcg ccagaagaat ccgggaaatg
    12481 caggaagaaa acgcgtaaac acgtttttcc ggggcatgga tgcggctgca tccggcagag
    12541 tggataaggg aaagcgcagg ttacatgaaa gaaaaaaata aaacaggaaa ctggctttta
    12601 aacgcagttt atccgagacg atgtcccatc tgtgacggac tgctgtcacg tgaggagctg
    12661 tatctatgcc ggaattgtgt ggaaaagatc catccgattc tgggcgcgcg gtgcaaaaaa
    12721 tgcgggaaac ccgtgggcag tgaggaagag ttctgccggg actgcagcag aacgaggcac
    12781 agcttcgacc gcggttttgc gccgtacggt taccacgggg aacttgaggc ttccctgatg
    12841 cggttcaaat atcatgaacg gcaggaatac gccggatttt atgctcacgc ggcgtttttc
    12901 tatgtgggaa agcagatcca gagaagaaat ccggaggtgc tcgttccggt gccgcttcat
    12961 ccggcgagac agcggaagcg cggctacaat caggcggagg tatttgcaga aaagctggca
    13021 gaagaaatgg aacttcccgt gaatgcccgc ctgatccgcc gtgtgaaaaa aacaaaacgc
    13081 cagaaagagc ttggtcccgc cgagcggcgg aaaaacctgg agggagcttt tgcattggca
    13141 aagccgggac agatgaaata ctgccggatt cttctggtag atgacattta cacaacggga
    13201 agcacggttg atgccattgc gtcactgttg aaagtgaatg gagcagaaca ggtggatttt
    13261 gtttgtgcgg caattgtaaa atagtgtgtg aatattttga gaatagcttt ccaatatcgg
    13321 aaacatatgg taaaataaac ataatttaga acgggaagag gagaccaaga ggcatgttgg
    13381 acgaaaaaag gattcgtcag atgacaaaac tggctttttt cgaaaaaaaa gaagaaaaaa
    13441 atgccttacg aatcggccgc tattttcgga gtgattacat cggaaaagag cttctgaaaa
    13501 atttttttct ggcttccatc ggatacattc tggtacttgc ggttgcagtc ttatatcata
    13561 ttgaatggtt ggtggacgag atagacacaa tcgacctgca gtttgcagca gggtgtctga
    13621 tcgcgtccta tctgtgtttt ctggcagtct attccattgc cgtgtatgtg atcagcacga
    13681 tccgttacca gggagcacag aaaaagctga aatggtatgg aaaagagctg gatgacctgc
    13741 agaacatgta tgaggaacag gaaaaaagcg ggaagaaatc cgggcgcagg aggaaggaaa
    13801 catgacagta ttgatcgaac tgaaagaaaa attaaaagag ttttacggct cctgcagcgg
    13861 aatcgtactt ccgattgtga aatttctgct cgctcttgct gtatttcgtg gtattaattc
    13921 caatctcggc tatctgaaga tgctgaacag catgtatgtg gtattgattc tgtcgctgat
    13981 ctgcgccatc ctgccattga atggaatggt tttccttgca atgctggtga tcatcggcca
    14041 ctgctttgca cttcacatgg taatcgcggg tcttgcggcg ctggttctgc tggtgctctg
    14101 gtttctatat cttcgctttg tgccgaagga tgctccggca ctccttttga caccgctcgc
    14161 attctggctt cacgtgccat ctgccgttcc ggttgcctat ggtcttgccg gcacgccgct
    14221 ctctgcattt tcagcagcct gcggtgtcgt tgtctactat atgtgcgaca tgattcacgg
    14281 aaaaatggag ccgctgcttc atgcggcgga agcaccggag atcacggcag tcgtacagga
    14341 atttttcaac ggcctcttcc gcaatgagga aatgctgctg gttctgatcg cctgcgcact
    14401 gaccgtactt cttgtcaatg caatccgcca cagcagtgcg gactatgcat ggcagatttc
    14461 cattgtggca ggttcggttg cctatgcggt tattatgatt gccggaagcc tggcactgga
    14521 tgtacagatt gcactcccga tggttctgat cggggcggcg gccggctgtc tggttggatt
    14581 tgttctggag tttttcctgt tcggtgcaga ctacacccgg acagaatacc tggaatatga
    14641 tgacgacgat tattattact acgtaaaagc cgttccaaaa ttcaatgtga caaaggcaca
    14701 gcgccgtgtg acttatattc ggaatgaaaa gaaaaaagaa ccggatctga cagacgagga
    14761 actcgatgct ctggcaaatc ttggacatac agaagaatcc gggaacagca gggatcttga
    14821 tgagatcgag gaaaaagaag aaatagaaga aataaacgaa acagaagaaa taaacgaaac
    14881 agaagaaatt caggaagaag aaattccgcc gctggacgag ggcgattatc aggatgtgga
    14941 ttataagagc atgctggagg acaccttaaa ggatctttaa aagaattttg aaaatcagaa
    15001 gaaaaccggc cgggggaaaa gaaaggaagt gacagtgtgc gtggcgtgtg gaactggatt
    15061 gtcacaagaa cggcagacta cgggctgcca aaacaaattg aactgattga tattgtgcag
    15121 attctgctca ttgcattttt cgtctatcac ctgatccgct ggctgcagaa tacaaaggcc
    15181 tatacgctga tgaaaggtat ttttctgatt ggtgtctttg tcatcattgc aaacgtgctg
    15241 aaaatgcaga cgattctgtg gctgttcgga aacatcggcg gggcggcgat cacagcggtt
    15301 gtaattatct ttcagccgga gcttcgaaga gtggtcgagc agattggaga aaagaatccg
    15361 ctgtctgcca tttcttttgc gagcggccag gaggaaaatg aacggttcag tgacaaaacc
    15421 atcaatgaac tggtaagggc cagttttgaa atgggagaag taaagaccgg agcgctgatc
    15481 gtcatcgaaa agaccatcac cctggaggaa tatgaaaaga ccggaatccc gattgacggc
    15541 gttctgacca gccagctgct gatcaatatt ttcgaacaca atacgccgct gcacgacgga
    15601 gccgtgattg tacggaacaa ccgtgtcacg gcagcaacat gttacctgcc gctgtccgac
    15661 aacatggatc tgaacaagaa tcttggaaca cgccaccgtg caggtgtcgg catcagtgag
    15721 gtgacggatt cctttacgat tattgtctcc gaggaaaccg gaaatgtgtc gtatgcttcc
    15781 ggcggagaac tccacacggc agtaacaccg agcgaccttc gggagcagct tcacaaaatc
    15841 cagaagctcg cgaaaaaacc ggaagagaag aaaggatggc atatccgcgg aatgcgtcag
    15901 aaagtggagg gagagaagaa atgaaaaaga aaattctgaa aaagcttctg aacaatctcc
    15961 ctttgaaaat tctttctatc ctgattgcca ttgtaatctg gtacgttgtg gtcagtgtga
    16021 atgacccgat tgtgaaagag cgctttgatg tccccgtgca ggtgaccaat gaagcctaca
    16081 tcgcagccgg aaagaaaaca taccagattg aggaggaata ccagacggtc acagttactg
    16141 tcacggataa caactcggtt gtcagccggc tgaaagcatc ggatatcacc gtcacggccg
    16201 atctgaccca gattgtgacg atggatacaa acccggttta tgtgccgatc aaggcagcgt
    16261 gctccatggt aaaacaggaa aaactgagca cagtgacagc aacgatccct gtggaaatcg
    16321 aggatgtgga cagcgaaaaa ttcccgatca caattgacgc aggaaatacg aaaccggcaa
    16381 aagattacga agtgggaacg atgacttccg atccggagag catcaccatt tccggcccga
    16441 cgtccctgat caataagatc agcagtgtgg tggcaaaagt agatgtaacc aatatgcgga
    16501 actccggaac cgtcacggcg gatctgatga ttattgataa aaaccaggat gagatgtcgg
    16561 aatcccagat ggaatttttg aatttcgaca gcggctcccc gcaggtggat gtggatattg
    16621 aactgtggcg ccgtgtatcc ggtattaagc tgagtgcact gtacagcgga actccggcag
    16681 acggctatca gattaaaaat atctatacca caccggagga aatcaccgtt gccggatccg
    16741 aggaggcact tgcaaagctg gcggatgaag gcaatacgat tgaaattccg gaagactata
    16801 cctccgttgc aggacaacgc tccgatgtgg agacgacggt tgacttaagc gatgttctcg
    16861 ccgatgtgac agatctgaaa gtatcctcca gttcgtctgc atccgttacc gttcacgtaa
    16921 cggttatgcc gaatgagagc agggaatttg aacttgatgt cgatcagatc gagacgagca
    16981 atcttcagtc cacttatacc gttctgtatg atcagacaca gcttgcaatc cgcattaaag
    17041 catcggacaa aaatctggcc aaattagata cttcccagat caacgcatcc atagatctga
    17101 atggaatggg tgtcggggat tacgaggtac cggtaaaaat cacactgccg gatggctatg
    17161 agctcgtaga cagtgtcaag acaacgattc atatcaaaga aaaagcagta gcaaataaaa
    17221 cgacaggaac aaattaggag ggaaaccaat ggtaagcgga aaagtgaccg tgaaaaatcc
    17281 aaccggcctt catcttcgcc cggccggaac cctgtgcaaa caggcaatgc agtttaaatc
    17341 atctattaca ttccactacg gagatggaaa tacagcaaat gcaaagagcg tcttaagcgt
    17401 ccttggcgca tgcgtcaaat gcggagacga aattgagctg ttctgcgagg gagaggacga
    17461 ggaggaagct ctgcagttcc ttgtcaattc catccggacg ggacttgggg aagaactgta
    17521 aggagaaaga acaatgaaga aatatgatta tgttctggtt ggcgccggtc tgtattccgg
    17581 agtgattgct tatcttgccg gtcaggcagg gaaaagctgt cttgtactgg aacgaagaga
    17641 tcatacaggc ggaaatattt actgtgaaga aatcgaggga attcatgtac acaagtatgg
    17701 agcgcatatt ttccatactt cgaataaaga ggtgtggcag tttgtcaatt cgctggcaga
    17761 attcaaccgt tatacaaact cgccgattgc caactacaaa ggcgaaattt ataatatgcc
    17821 gttcaatatg aataccttca gtaaaatgtg gggaatccgc acaccggaag aggcgaaaac
    17881 aatcatcgaa gagcagagaa aagctgttcc gggagagccg aaaaatctgg aagagcaggc
    17941 gatcaaactg gttggaaaag atatttatga aaagctggtt aagggttata cagaaaagca
    18001 gtggggaaga gactgcagtg agctgccgag ctttatcatc aaacggcttc cggtgcgctt
    18061 tacgtatgac aacaactatt tcaatgacct ttatcaggga attccgattg gcggttataa
    18121 cgttctgaca gagcggcttt ttgaaaaagc ggatgtacag acaggtgttg attttctgga
    18181 agataaagaa aagtacctgg cgatgggtga gacggtgatt tacaccggag cgattgatgc
    18241 gttttacgat tatgcacttg gcaagctgga ataccgtacg gtgcgctttg aaacagaagt
    18301 gctggatacc gacaattatc agggcgtagc tgtagtaaac tacaccgatc gggagacacc
    18361 gtatacgcgt gtgatcgaac acaagcattt cgaattcgga acccagaaaa agaccgtcat
    18421 ttccagagag tattccaccg actggaaaga ggggatggag ccgtactacc cggtgaatga
    18481 tgccagaaac caggaacttt atcagaaata tgccgcactt gcggaaaaag aagagaaggt
    18541 tatctttggc ggaagacttg gagaatacaa gtattacgat atggacaaag tcattgaggc
    18601 tgcactgaac cagtgggaga aaatgcagcg gtaggaaaaa gaactgcaaa cggaaatcaa
    18661 tcaaaacgga ctgcacactt gacagcagcc cgttttttga ctatattata aacgatatcc
    18721 ggctgaaaaa taccggaaaa taagaaaatg aaagaggtcg ttatgagaaa agaatatgat
    18781 gacgaggatg aaatcccgga aagacgccat tcctcaggaa agaaaaagaa gaaattcggc
    18841 tgggttaatg tgctcaccat ccttctcgta gttgtactgg ttgtgctcgt cggtgtctat
    18901 gctgtaatca atcactatta cagcaagagt aactacgtaa gcgatgataa aatcacgatc
    18961 aataaatcca cagaggttga gaccgaaagt accgggctga ccgatgagga gacggatgcc
    19021 ctgcagtctg agattttaaa tgagacacag gacattgaac ttccaaacaa caataacgtg
    19081 tacaacattc tgctgatcgg tgttgaccgt cgtgacaaaa cgtggtatgg aaactc
//
LOCUS       DAOMUF010000018        18902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_518_length_18902_cov_2.888576, whole genome shotgun sequence.
ACCESSION   DAOMUF010000018 DAOMUF010000000
VERSION     DAOMUF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 18902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18902
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_518_length_18902_cov_2.888576"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLN77_02310"
     CDS             <1..435
                     /locus_tag="PLN77_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM43149.1"
                     /translation="GVPSVDLPDAKKRKVFGYVEQSFHMVPGSVKDQITLYDAKISDT
                     DVKKAAELTGLAKTIEALPDQYDTICTQDLFSQGQWQLLSIARAAAAEPEILLLDEIT
                     ANLDAETEKMVLNALQRVSKNRTVISISHRTMAETGRVIPIS"
     gene            624..1721
                     /locus_tag="PLN77_02315"
     CDS             624..1721
                     /locus_tag="PLN77_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43150.1"
                     /translation="MKKRKWKFRIAGGAVTLLGIYLMAVGYGETITLTIATVVLIFGI
                     AIWSMATPESYNSMTDMIAMISMEKPRKIEEFYEAYKNVDTPFGSAWLAKFYTMRQKA
                     LVFGPDAKGEYLYFWLTKDGHVGYLGYSFIEDFIKKKLTTPVYPIHEDVAENLADHLS
                     YHSDLMMFQSELKANLEHFVKNGTVQPFQKISASQIYTFTEDYRLTGQHFDLEDTDGN
                     LVYEIDSTVPLKTFYIYDAMHTEIFRMTKELLHALPTYRFYLYGEPYGVLKKQFALVR
                     DQFSMELPEGKLELREYAGSIGHNYSVKLNGTMIGAIVDNMDLTVGNIMFDNAFLIVY
                     DAKYLPQLTALAVMAARELARDKDGGLSNRS"
     gene            1744..2409
                     /locus_tag="PLN77_02320"
     CDS             1744..2409
                     /locus_tag="PLN77_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43151.1"
                     /translation="MRKTTKLFLGFTAGLLLLCGCSAAEEKLGYENADVAKEALASAS
                     SVRMRCDLDNVDASGNIYADEKAAAYMKNSGLFDQTWTISISGEEWFHMKIVTDEEIN
                     KMTKSATTYAFYDPDNNLLGYAQERVTTPDQMPEAYYIIFLDADLNEKPYYASEDGHT
                     IYTEGGTVIGSAKKEMDWSGSQCNLYVNLEDNGTQVMDFQDKYALLDMMYDEANEYYK
                     DNK"
     gene            2428..3195
                     /locus_tag="PLN77_02325"
     CDS             2428..3195
                     /locus_tag="PLN77_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43152.1"
                     /translation="MKKRIYTAAGLFLAAALVLGGCGKEKVPESEFATADEAKEALTS
                     AETIEIYFDLYGSKRKTKIVADGKVAGYLQKNTVYINEEEWFSIDYVEDEPINDREGS
                     GTTYGYYDASGTCLGYAQEQYVDTSYGKDTRLIFLDVDETPLDYQSSRSGRILYDEND
                     EEIGSGKIGRSGVSLLDQWYTNLYCAVFKTETDGTRQIDFMDRMAMLWRLERDYRSID
                     DNPVNTGVQIYETVAVVFVLILLIGSSFSKWRQKKKN"
     gene            3476..6112
                     /gene="mutS"
                     /locus_tag="PLN77_02330"
     CDS             3476..6112
                     /gene="mutS"
                     /locus_tag="PLN77_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRM43153.1"
                     /translation="MMVHYCQTKTGLTDPANTILFYRLGDFYEMFFEDATLVSKELEL
                     TLTGKDCGMEERAPMCGIPYHAAEGYINRLVENGHKVAICEQMEDPKQAKGMVKREVI
                     RVVTPGTTLIPQGLDETKNNYIMSIVCMEGCFGISTADISTGQCLVTEMDKPRRLVDE
                     ITKFMPAEIICNQSFLLCGIDVEDLRTRLHICVNALEDWYFDDDICRRTLQEQFHAAT
                     MEGLGIADYNCGTIAAGALFQYLHETQKSDLSHMNSITPYVTDKYMLIDSSSRRNLEL
                     VETLREKQKRGSLLWVLDKTRTAMGARLLRSYVEQPLIDEDEINARLDAVEALTGQAM
                     IREEIREYLRPVYDLERLVGRISYQSANPRDLIAFKSSLQMLPYIKQLLNSFDGGALA
                     QIQEDLDPLEDLYQLVDAAIVDDPPYLMRDGGMIKDGYNEMVDNYRHAKTEGKQWLSE
                     LEASEREKTGIKNMKVKYNKVFGYYLEVTNSFKDLVPDYYTRKQTLTNAERYITPRLK
                     ELEDTILGAEDKLYALEYDLFCTVRDTVAGEAERIQRTAQALARLDVYASLSYVAEHN
                     HYVRPKLNSRGKIDIKDGRHPVVEKMIPNDMFIANDTLLDNGDHRVSVITGPNMAGKS
                     TYMRQTALIVLMAQIGSFVPASKANIGIVDRIFTRVGASDDLASGQSTFMVEMTEVAN
                     ILRNATSNSLLILDEIGRGTSTFDGLSIAWAVIEHVANPKLLGAKTLFATHYHELTEL
                     EGKLPGVHNYCIAVKEKGDDIVFLRKIVQGGADKSYGIQVARLAGVPESVLSRAQELV
                     EQLSDADITAAVKDLTMQKKPKAKPVHYDEVDMAQMSLFDTVQDDDIIKELSELDISN
                     MTPMDALNTLYRFQGKVKNRWK"
     gene            6168..8198
                     /gene="mutL"
                     /locus_tag="PLN77_02335"
     CDS             6168..8198
                     /gene="mutL"
                     /locus_tag="PLN77_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRM43154.1"
                     /translation="MATIAVLDQNTIDKIAAGEVVERPASVVKELVENAMDAGATAIT
                     VEIRDGGISLVRITDNGSGIPAEQVPLAFLRHATSKIRSVEELVQIASLGFRGEALSS
                     ISAVAQIEMITKTPDALTGTRYVIEGGAEKANEEVGAPTGTTFLVRNLFYNTPARQKF
                     LKTPVTEANAVTSVVEQLALSHPGISFKYMVNSQVKLHTSGNRNLKELVYNIYGRDIA
                     RELIEIHSESPLMTIDGFIGKPVISRGNRNFENYYVNGRYVRSKLLARAIEDGYQTSM
                     MQHKYPFTLFYVQMDGEAVDVNVHPTKMELRFSHQEEIYRQMRDMISGALNHREQIVK
                     VSLSSDRERKAEEKAARKEQIVVPEPFEQKRQVKEGFARQSLPVEPVSSQSRRMPIFP
                     EVERNGYRAAKTYVPVQQMTGMTREEEELFEPGAGNVPKTADLSNADAIQHQQEPDAA
                     PTVSSMPEESSSASASPEPEGQMQLFDEQFLTPEAEKSHRIIGQVFDTYWLIQYGENL
                     YIIDQHAAHEKVLFERMMKNYREKNVTSQMVSPPLIVSLTPQEADLVTRYMDVFQSFG
                     YEISPFGGKDYAINAVPHNLYGIATEELFIEILDNLEEVREKPLEILKDRLATMSCKA
                     AVKGNNHLSYAEAEQLIAELLTLEDPYHCPHGRPTIISLSKRELEKKFKRIV"
     gene            8208..9185
                     /gene="miaA"
                     /locus_tag="PLN77_02340"
     CDS             8208..9185
                     /gene="miaA"
                     /locus_tag="PLN77_02340"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRM43155.1"
                     /translation="MKKPLIILTGPTAVGKTKLSISLAKAVNGAIISADSMQVYRHMD
                     IGSAKIRPEEMCGVPHYLIDVLDPSEEFHVVKFVELAHEAMEKIYAAGQIPIVTGGTG
                     FYIQALLKEVDFTESHGELPIRKQLEEKAATEEGAAELYRELSEVDPASAETIHPNNV
                     KRVIRALEYYHETGQKISEHNEEQKQKESPYCSAYFVLNDERSILYDRIDRRVDQMIR
                     DGLVDEVFRLKEMGYTRDLVSMQGLGYKELFPYLAGECTLEEAVYIIKRDTRHFAKRQ
                     LTWFRREKDVIWLNKPDFDYDEEKILSYMLDRWNEIVSADAKEEGEQSC"
     gene            9179..10465
                     /locus_tag="PLN77_02345"
     CDS             9179..10465
                     /locus_tag="PLN77_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HRM43156.1"
                     /translation="MLTEKAAMYSTLGISKEVFAFGTKIEEELKERFAKIDEIAEYNQ
                     LKVIHAMQASKVSEACLYGTTGYGYNDLGRDTLEQVYATCFGTEDALVRPQIACGTHA
                     LATALSGNLRPGDELLSPVGKPYDTLEEVIGIRPSVGSLAEYGVTYRQVDLKEDGSFD
                     YEGIKNAINKKTKMVTIQRSKGYQTRPTLSVTRIGELIAFIKNIKPDVICMVDNCYGE
                     FVETIEPTNVGADLMVGSLIKNPGGGLAPAGGYIVGKKKYVENAAYRLLSPGLGKEVG
                     ATLGVNGSFYQGFFLAPTVTAAALKGAVFAANVNEKLGFAVVPNGTESRHDIIQAVTF
                     GKPEGVIAFCQGIQAAAPVDSFVSPEPWDMPGYDSPVIMAAGAFVQGSSIELSADGPI
                     KPPYAVYFQGGLTWQHAKFGILMSLQKLVDAGMVTL"
     gene            10465..11652
                     /locus_tag="PLN77_02350"
     CDS             10465..11652
                     /locus_tag="PLN77_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRM43157.1"
                     /translation="MAKNVVIIGGGASGLMAAIWASRLGAAVTVLEKNDKPGRKLLAT
                     GNGRCNFTNRYQDASCYRTGNQERASRVLEQFTEQDTEQFFEQLGIRIRSREGWLYPA
                     SEQAQSVLDLLVLEARFRKVKIKTRETAVAVEKAENGYLVRTEGWQYPADSVIICCGS
                     CASQIAGSDGDTLRFADQLQLASIPFSPALCPLRCKGNQFPSWAGVRVRAKITLLVEN
                     QEILTEQGEMQLTDYGVSGIPVFQISRFAVRACMQKKRAELLVDFFPELTESELTAEF
                     VRRQEICPYKAPKELLIGLLPEKLIPVLIGKKKTPEEMAGAIKSYRLQITGQTDFGKA
                     QVCAGGITLDQLTDSLESVQHPGIFFAGEALDVDGVCGGYNLQWAWSSGSAAGRAAAG
                     EHA"
     gene            11649..13262
                     /locus_tag="PLN77_02355"
     CDS             11649..13262
                     /locus_tag="PLN77_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM43158.1"
                     /translation="MIRIQQLKLPVTHTQEELEQKIRKTLKIGKHDLQEIELLRRSID
                     ARKKSELKYVYQLDVKVKDENAVLRKAKNNQIVKAEKKSYVFPKSGEQTLVHRPVIIG
                     SGPAGLFCAYVLAKHGYRPLVLERGESAEQRKKSVDAFWKTGVLNPESNVQFGEGGAG
                     TFSDGKLNTSVKDPSGRNREVLRIFTECGAPEEILYDQKPHLGTDQLIGIVTTMRKKI
                     EAWGGEVRFGAKVTDFGIENGRLTSVTVNETEKIAAETAVLAIGHSARDTFFKLCENK
                     ISMEAKSFAVGVRIEHPQKMITEDQYGPEAPDFLGAAPYKLTNQCENGRGVYSFCMCP
                     GGYVVNASSEAERTCVNGMSYSDREGKNANSAMIVTVTPEDYRPYHVEGTPDVLDGVA
                     FQRALEHAAWEAGKGKVPVQLFGDFCENRVSTALGEVTPSICGEWTFANLREVLPTFI
                     GDSLVEGIRASERKIHGFSRPDAVLSGVEARTSSPVRIVRNETLESSSLTGLYPCGEG
                     AGYAGGITSAAMDGLKTAEEIAKKYMNFS"
     gene            13357..>13769
                     /locus_tag="PLN77_02360"
     CDS             13357..>13769
                     /locus_tag="PLN77_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43159.1"
                     /translation="MRKNGLRNLPTMEQPYEKCLENGPESLSDAELLAVILRCGARGK
                     TSLELAREILSLPGKEEGLLGLYHCTRQELLQIRGVGEVKAIQLQCIGELSKRMAAAG
                     ARKGLCFHDPASIADYYMERLRHEDQEVLLCMMLDT"
     assembly_gap    13770..13779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13780..14035
                     /locus_tag="PLN77_02365"
     CDS             <13780..14035
                     /locus_tag="PLN77_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRM43160.1"
                     /translation="KEITRGTVNATMISPRELFLAALQFRAVHILLVHNHPSGIPEPS
                     GDDITVTRQIRRVGEMLGITLLDHIIIGDHCYTSMLEQGI"
     gene            14092..15105
                     /locus_tag="PLN77_02370"
     CDS             14092..15105
                     /locus_tag="PLN77_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRM43161.1"
                     /translation="MQIRNVYGIDLGTSTVKIYDLKKDTITKEKNMIAIRNREQVIAV
                     GNYAYEMHERTPSNIEVITPMSNGRIANVLMVEAVLHTLLHRCTRHMGYRPELYFSVP
                     CDMTEIERRSYYTIAHKGALKNCRMYLVDKPIADALALGIPINRTKGSMIVNIGAQST
                     EISVIANARVIFSRIIPVGGKQFNESICNLNRRKNNFQIGSKTAKRVKIALADLGTDK
                     KEARKVRGVDGASGLPMEGIITSSLVNEALLSGVNEIGEEIKHALERTPPQIHDHIQK
                     EGIYITGGSTRIPNIDRYLSRQLGCPVQLSQYYDLCTICGLKELITHDALHRWAYTVN
                     KKK"
     gene            15127..15978
                     /gene="mreC"
                     /locus_tag="PLN77_02375"
     CDS             15127..15978
                     /gene="mreC"
                     /locus_tag="PLN77_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRM43162.1"
                     /translation="MNKKKKFQLKSKHLLFIMTFLCLSGILGSAADKMPVTPIREAAG
                     FVIIPFQNGINQIGAYLTDCLTGFQNVKKLSAEKEELEQKVTDLTEENNQLVQDQQEL
                     QRLQELYQLDQSYSEYEKVAAQVISKDPGNWYDTFVINKGSNDGIQKDMNVIADGGLV
                     GLVEEVSSNSATVRSIIDDSSSVSAMTASTSDTCIVSGDLRLISDGKLAFSQLNTTDT
                     VAEGEKIVTSNISDKYLRGILIGYVSEITEDSNHLTKKGYIIPVVDFQHIQEVLVIKE
                     LKQQGGE"
     gene            15979..16538
                     /gene="mreD"
                     /locus_tag="PLN77_02380"
                     /pseudo
     CDS             15979..16538
                     /gene="mreD"
                     /locus_tag="PLN77_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221849.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
     gene            16523..>18902
                     /locus_tag="PLN77_02385"
     CDS             16523..>18902
                     /locus_tag="PLN77_02385"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM43163.1"
                     /translation="MAKKIRKFFRRLGENRTLLLGVVFLAMFAVLIGRLFILQVVHGE
                     EYADNFELQITKTRTLESTRGNIYDRNGKLIAGNKVSYSVTIEDNGTYNTTKERILSL
                     NAELYRLCKLIRANGDSIDTSTFPIEVDENGAFVFTGEEGTTRDRFRADIYGQKKIDD
                     MTAEQKSSSAETLMTFLAGPDGFGLDAYSDDEKYAYSAKDFEEYGLPYQTDESGNAVL
                     SLSNQERLEILVIRYKMKQTNYQKYVRVTVASGVSSNTTASIAENEDVLQGVSISEDS
                     ERVYYDGKYFSSLIGYTGQASSDELTELNEELKSQGKEETYSTESIVGKSGLEQYMET
                     ILQGTDGSEEIIVNNVGKELETVEGSLVEPKQGNNVYLSIDYDLQIAVYKILEQRIAG
                     ILKQYLSDVKSVDTSTLANTDAVPIPIYDVYNALIENSTIDISHFSAADATEREQRIY
                     ALFQQKLQQVLAEITQELTVETATVYNDLSDEMQEYETFIVDKLLSSTMGILSSTAID
                     EKDATYQAWNDGTISLRDYLTYAASQNWIDVSALTQDDAYLDSQEVYQKLTEVILDRL
                     ANNTTFAKKMYHYMLLQDMLDGYDICNLMYDQNLLTKEDDDYAAYVAGNMTPFQLLSD
                     KIAKLEITPAQLALDPCSGSAVITDPNTGAILACVSYPGYDNNRLANQMDTAYWAKLN
                     TDESSPLYNKATQQKTAPGSTFKPLMAVAGLSEGIITPTSTINCNGLFGEGLVNESDY
                     VHCHQLSGHGDLNIVGAIQNSCNVFFCTLGYRLGLDENGTFTQKRSLEMIQKY"
BASE COUNT         5127 a         4433 c         5062 g         4270 t
ORIGIN      
        1 ggcgttccat ctgtcgatct gccggatgcg aagaaacgaa aggttttcgg ctatgtggag
       61 caatcgttcc acatggtgcc gggatcggta aaagaccaga ttactttgta tgatgcaaag
      121 atttcggata cagatgtaaa aaaagcagca gagctgaccg gacttgcaaa gaccatcgag
      181 gcgcttccgg accaatatga tacgatctgt acacaggatc tcttctcaca gggtcagtgg
      241 cagctcctct cgattgcccg tgcagccgca gcggagcctg aaattctgct cctcgatgag
      301 attaccgcaa accttgatgc agagacggaa aaaatggtat taaatgcatt gcaaagggtt
      361 tccaaaaacc gtaccgtaat ttccatttcc caccgaacta tggcagaaac gggacgcgtg
      421 attccaattt cataatttct tttcgttcat taaatttttg aaacaaagcg tgccggatgc
      481 attctgacaa aaaaagatgc gccgacacgc tttctttgtc ccgccaggca gaacaaaata
      541 gagcggagga gcgagtttca attgacacaa aaaaggggag caagtacaat aaaagtaacg
      601 aaaagagagg aggcggtatt cagttgaaaa agagaaaatg gaaattccgc atcgcaggcg
      661 gcgctgtcac gctgcttggc atctatctga tggcggtcgg ctacggagag acgatcacac
      721 tcaccatcgc cacggttgtc ctgattttcg gcattgccat ctggtccatg gcaaccccgg
      781 agagctacaa ttccatgacg gatatgattg ccatgatctc catggagaag ccgcggaaaa
      841 tcgaagaatt ttatgaggct tacaagaacg tcgatacacc gttcggttcc gcgtggcttg
      901 caaaattcta cacgatgcgg cagaaagcac tcgttttcgg accggacgcc aagggagaat
      961 atctctactt ctggctgacc aaagacggcc atgtcggcta tttgggatat tccttcatcg
     1021 aagactttat caaaaagaaa ctcaccacac cggtgtatcc gatccatgag gatgttgcgg
     1081 aaaacctcgc agaccatcta agctaccatt ccgatctgat gatgttccag agtgaattaa
     1141 aagcgaatct ggagcatttt gtcaagaacg gaacggtaca gccgtttcag aagatttcgg
     1201 cgtcccagat ttacactttc acggaggatt accgcctgac gggccagcat ttcgatctgg
     1261 aggatacaga cggaaacctg gtttacgaga tcgacagcac cgtgccgctg aaaacatttt
     1321 atatctacga tgcgatgcac accgaaatct tccgtatgac gaaggaactg ctccacgcac
     1381 tgccgacgta ccgcttttat ctgtacggcg agccgtatgg tgtgttgaaa aagcagtttg
     1441 cactcgtacg ggatcagttc tcgatggaac tgccggaggg aaaactggaa ctccgggaat
     1501 acgccggctc catcggccac aattactcgg taaaactgaa cggaactatg atcggcgcca
     1561 tcgtcgacaa tatggatctt accgtcggaa acatcatgtt cgacaatgca tttctaattg
     1621 tctacgatgc aaaatatctg ccgcagctga cagcactcgc tgtgatggcg gcaagagaac
     1681 tggcacgcga caaggacggc ggtctttcca atcggtcctg acaaaagaaa gagaggggaa
     1741 tctatgagaa aaacgacaaa attgtttttg ggttttacag caggacttct gcttctgtgc
     1801 ggatgctcgg cggcagaaga aaagttaggg tatgaaaacg cggatgtggc aaaagaagcc
     1861 cttgcatcgg cttcgtccgt ccgcatgcgc tgtgatctgg ataacgttga cgcttcggga
     1921 aacatttatg cggatgaaaa ggctgccgcg tatatgaaaa acagcggtct ctttgatcag
     1981 acctggacga tctctatttc cggcgaagaa tggttccata tgaaaattgt gacggacgaa
     2041 gaaatcaata agatgacaaa atccgctact acctatgcat tctatgatcc ggacaacaac
     2101 ctgcttggct atgcacagga gcgcgtgacg acaccggatc agatgccgga agcgtattac
     2161 attatttttc tcgatgcaga tctgaacgaa aaaccatatt acgcatcaga ggatggacat
     2221 accatttata cagagggcgg aacggtgatc ggttctgcga aaaaagagat ggactggtcc
     2281 ggttcacagt gcaatctcta tgtgaacctg gaggataacg gtacgcaggt gatggatttc
     2341 caggataaat acgccttgct cgatatgatg tacgatgagg caaatgagta ttacaaagat
     2401 aacaagtaag gcggaggaaa aaggcagttg aaaaagagaa tatatacagc cgctggtctt
     2461 tttcttgccg ccgctctggt gcttggcggc tgcggaaaag aaaaagtgcc ggaatctgag
     2521 tttgcaactg cggatgaggc aaaagaggcg cttacatcgg cagaaaccat agaaatctac
     2581 tttgacctgt atggaagtaa acgaaagacg aagattgtgg cagacggaaa agtggcgggc
     2641 tatcttcaga agaatacggt ctatatcaac gaggaggagt ggttttcgat tgactatgtg
     2701 gaggatgagc ccatcaatga cagagaaggc tccggcacga cgtacggcta ttacgacgca
     2761 tccggtacgt gtcttggcta tgcgcaggag cagtatgtgg atacctccta tggaaaagat
     2821 acaagactga ttttccttga tgtagatgaa acgccgctcg attaccagag ttcgagaagc
     2881 ggcaggattc tgtatgatga aaatgacgag gagatcggca gcggaaagat cgggcgatca
     2941 ggggtcagtc ttcttgacca gtggtacacg aatctttact gtgccgtctt taagaccgag
     3001 acggacggca cgcggcagat tgatttcatg gaccggatgg cgatgctgtg gcgtctggag
     3061 cgcgattacc gctcaattga cgacaatcct gtcaacaccg gcgtgcagat ttatgaaacg
     3121 gttgccgttg tttttgtgct gatcctgttg attggttcgt ctttctcaaa atggagacaa
     3181 aaaaagaaaa attaaaggga atgctcgctt cctggacggc tggttttgag aaaaaaccac
     3241 tgacaggaag ggggcatttt atacgcttgt gaatcttgca caatttctgg tttcatgcta
     3301 aaattgtgca ggtataacgt ttgttatact tcttatattt attcttgaga aaaggtgtga
     3361 aatacaaatg aacgatacag aaacaaaaaa cacagcggta aaagcgccgg cactgatgtc
     3421 cacgcggcct gtgacagcac aggatgcgct ggatcagtgt gagaagcttt cgcctatgat
     3481 ggtacattat tgccagacga agacgggctt gactgacccg gctaatacca ttttgtttta
     3541 ccgcttaggt gatttctacg aaatgttctt cgaggatgcc accctggtat ccaaagaact
     3601 tgagcttacg ctgaccggaa aggactgcgg catggaggag cgcgctccga tgtgcggtat
     3661 cccgtatcac gctgcagaag ggtatatcaa ccgtcttgta gagaacggcc ataaggttgc
     3721 gatctgcgag cagatggagg acccgaaaca ggcaaagggc atggtaaagc gcgaagtcat
     3781 ccgcgttgtc acaccgggta cgacgctgat cccgcaagga cttgatgaga cgaagaacaa
     3841 ctacatcatg tcgatcgtct gcatggaggg atgcttcggt atctctacag cagatatctc
     3901 gaccggccag tgcctcgtca cggaaatgga caagccgcgc cgccttgtgg atgagatcac
     3961 gaagtttatg ccggcggaaa tcatctgcaa tcagtcgttc ctgctctgcg ggatcgatgt
     4021 ggaggatctt cggacccgcc ttcatatctg cgtgaatgca ttggaagact ggtatttcga
     4081 cgatgatatc tgccgccgta cgctgcagga acagttccat gctgctacga tggaaggcct
     4141 tggcatcgcc gattacaact gcggaacaat tgcagccggt gcgctgttcc agtatctgca
     4201 cgagacacag aaatcggatc tgtctcacat gaattcgatt acaccgtatg tgacagacaa
     4261 atatatgctg atcgacagct ccagccgccg gaatctggaa ttggtggaaa cgctgcggga
     4321 gaagcagaaa cgcggctccc tcctctgggt tctcgacaaa acccgcacgg cgatgggcgc
     4381 gcgtcttctg cgaagctacg tggagcagcc attgatcgac gaggatgaaa tcaatgcaag
     4441 actcgatgct gtagaggctc taacggggca ggcgatgatc cgcgaggaaa tccgtgaata
     4501 cctccgtccg gtttatgacc ttgagcgtct cgtcggaaga atcagctacc agtccgcgaa
     4561 tccgcgtgac ctgatcgcct ttaaatcgtc gctgcagatg ctgccttaca tcaagcagct
     4621 cttaaactcg tttgacggcg gcgctttggc gcagattcag gaggatctcg atccgctgga
     4681 agacctctat cagcttgtcg atgcggcaat cgtcgatgat ccgccgtatc tgatgcgcga
     4741 cggcggtatg atcaaggacg gctacaacga gatggtcgat aattaccgtc acgccaagac
     4801 cgagggcaaa cagtggctga gtgagcttga ggcaagtgag cgcgagaaaa ccggcatcaa
     4861 aaatatgaag gtcaaataca acaaggtatt cggctattat ctggaggtca cgaactcgtt
     4921 taaagatctt gtcccggatt actatacgag aaaacagacg ttgacgaatg cggagcgcta
     4981 cattaccccg cgtctgaagg aactcgagga tacgattctt ggcgcagagg acaagctgta
     5041 cgcgctggaa tacgatcttt tctgtacggt gcgcgatacg gttgccggtg aggcggagcg
     5101 gattcagcgc acggcgcagg ctctggcacg gctcgatgtg tatgcatcgc tttcctacgt
     5161 tgccgagcac aaccactatg tgcgtccgaa gctgaacagc cgaggaaaga ttgatatcaa
     5221 ggatggccgt cacccagtgg tcgagaagat gattccaaac gatatgttta tcgcaaacga
     5281 taccctgctt gacaacggtg accaccgcgt ttccgtcatc accggaccga acatggccgg
     5341 taaatcgacg tatatgcgcc agacggccct cattgtgctg atggcacaga tcggttcgtt
     5401 tgttccggct tcgaaggcaa acatcggtat tgtggaccgg atctttaccc gtgtcggcgc
     5461 atccgatgac ctggcaagcg gccagagtac gtttatggtt gagatgacgg aggtggcaaa
     5521 tattcttcgg aatgcaacct cgaacagtct gctgattctc gatgagatcg gaagaggaac
     5581 gagtacgttc gacggtcttt cgatcgcctg ggcggtgatt gagcatgtgg cgaatccgaa
     5641 gctgctcggc gcaaagacac tgttcgctac gcattaccat gagctgactg agcttgaagg
     5701 aaagcttccg ggcgtgcaca actactgtat tgccgttaag gaaaagggcg acgatatcgt
     5761 attcctgcgt aaaattgtac agggcggcgc agataagagc tacggtatcc aggtagcgcg
     5821 tcttgcggga gtgccggagt cagtgctctc ccgggcgcag gagctggtgg agcagctgag
     5881 cgatgccgat atcactgctg cggtcaagga tctgaccatg cagaaaaaac cgaaggcaaa
     5941 gccggttcac tacgacgagg tcgatatggc acagatgtcg ctgttcgata cggtgcagga
     6001 cgatgatatc atcaaagagc tctcggagct tgatatctcg aatatgacac cgatggatgc
     6061 tttaaatacc ctgtaccgtt tccagggaaa ggtgaaaaac cgctggaaat aggggaatac
     6121 tggatcagaa cataagttag aatcaatccg aaacagaaag gaacaacatg gctaccatag
     6181 cagtactgga tcagaatacg atcgataaaa ttgcggccgg tgaggtcgtg gaacggccgg
     6241 cttccgtggt aaaggagctg gtggaaaatg caatggacgc cggtgcgacg gcgatcaccg
     6301 tggaaatccg cgacggcggt atctcgctgg tgcggatcac ggacaatgga agcggcattc
     6361 cggccgaaca ggtgccgctc gcttttctgc gccacgcgac gagcaagatc cgttccgtgg
     6421 aagaactcgt gcagattgca tctcttggct tccgcggcga ggcgctctcg agtatctcgg
     6481 cggttgcgca gatcgagatg attacgaaaa cgccggatgc gctgacggga acgcggtatg
     6541 tgattgaggg cggcgcggag aaggccaatg aggaggtcgg tgccccgacc ggaacaacct
     6601 ttctggtccg gaatcttttt tacaacacgc cggctcgtca gaaattctta aaaacgccgg
     6661 ttacggaggc gaatgcggtg acttctgtgg tggagcagct tgcgctgtcg catccgggga
     6721 tttcgtttaa atatatggtg aattcacagg tgaagctcca tacgagcggc aaccggaatt
     6781 taaaagaact ggtctataat atttacggcc gcgacattgc gcgggagctg atcgagattc
     6841 actcggaaag cccgctgatg acgatcgacg gctttatcgg aaaaccggtg atctcgaggg
     6901 gaaaccggaa ttttgaaaat tattacgtga atggacggta tgtgcggagc aagctgctcg
     6961 cccgtgcaat tgaggacgga tatcaaacat cgatgatgca gcataaatat ccgtttacgc
     7021 tgttctatgt ccagatggat ggagaggcgg tggatgtcaa tgtgcatccg acgaagatgg
     7081 aacttcgttt ttctcatcag gaggagattt atcgccagat gcgcgatatg atttcaggtg
     7141 cgctgaatca cagggagcag attgtgaagg tttcgctttc ttccgacagg gaacggaaag
     7201 cagaggaaaa agcggcacgg aaagaacaga ttgttgttcc ggaaccgttc gagcagaaac
     7261 ggcaggtaaa agaaggcttt gcacggcagt cccttccggt ggagccggtt tccagccaga
     7321 gcagaagaat gccaattttc ccggaggtgg aacgaaatgg ataccgcgca gcgaaaacgt
     7381 atgtgccggt tcagcagatg acgggaatga cgcgggaaga ggaagaactg tttgagccgg
     7441 gtgcaggaaa cgtgccaaag acggcagacc tctcaaacgc ggacgcaata cagcatcagc
     7501 aggagccaga tgccgctcca acggtatctt ccatgccgga agaatcctca tctgcatctg
     7561 cttcgcctga gccagaaggc cagatgcagc tctttgatga gcagtttctt accccggagg
     7621 cagagaaatc gcaccgcatc atcggccagg ttttcgatac gtactggctg attcagtacg
     7681 gcgagaatct ctacatcatc gatcagcatg ccgcgcacga aaaggtactg ttcgagcgga
     7741 tgatgaaaaa ttaccgggaa aagaacgtca cctcccagat ggtgagtcct ccactgatcg
     7801 tctccttaac gccgcaggaa gcggatcttg tgacccgtta catggacgtt ttccagtcct
     7861 tcggctacga aatttccccg ttcggcggaa aagactatgc gatcaacgcc gtgccgcaca
     7921 acctgtacgg tattgcgacg gaggagctgt ttatcgagat tctcgacaat cttgaggagg
     7981 tccgcgaaaa gccgctggag attttaaaag accgtctggc gacgatgtcg tgcaaggccg
     8041 ccgtaaaggg caacaatcat ctttcctatg cagaggcgga gcagctcatt gccgagcttt
     8101 tgacgctcga agatccgtat cactgcccgc acggccggcc gacgatcatt tccctctcga
     8161 aacgggaact ggaaaagaaa tttaagagaa ttgtgtaggt gatttttttg aaaaaacctt
     8221 tgattatcct gaccggtccg accgctgtcg gcaagacgaa gctgtcgatt tcgcttgcca
     8281 aagcggtgaa tggggccatt atctccgcgg attccatgca ggtctaccgc catatggata
     8341 tcggctctgc caaaatccgc ccggaggaga tgtgcggcgt tccgcattat ctgatcgatg
     8401 tgctcgatcc ttccgaagag tttcacgtcg tgaagtttgt ggaactggcg catgaagcga
     8461 tggaaaaaat atacgccgcc ggacagattc cgatcgtgac gggcggcacc ggcttttata
     8521 tccaggcgct tttaaaagaa gttgatttta cggaatcgca cggtgaactg ccgatccgga
     8581 aacaactgga agaaaaagca gcaacggagg aaggcgcggc agaactgtac cgggaacttt
     8641 ccgaggttga tcctgcttcc gcggagacga ttcacccgaa taatgtaaaa cgtgtaatcc
     8701 gggcgctgga atattatcac gagacaggac agaagatttc ggagcacaac gaggagcaga
     8761 agcagaagga atctccgtac tgttctgctt attttgtgct gaacgacgaa cgttcgattc
     8821 tgtatgaccg cattgacagg cgcgtcgatc agatgatccg cgacggactg gttgatgaag
     8881 ttttccgttt gaaagaaatg ggatacacaa gagatcttgt ctccatgcag ggactgggct
     8941 acaaagaact gttcccgtat cttgcgggcg aatgcacgct ggaggaggcc gtctatatca
     9001 tcaagcggga cacccgtcat tttgccaaac ggcagctgac ctggttccgc cgggaaaagg
     9061 atgtgatctg gctgaacaag ccggattttg actacgacga agaaaaaata ttatcatata
     9121 tgctggatcg ctggaacgaa atcgtttcgg ccgatgcaaa ggaagaagga gaacaatcat
     9181 gttgacagaa aaagctgcta tgtacagcac acttggcatt tccaaagagg ttttcgcatt
     9241 tggaacgaaa attgaggaag aattaaaaga gcgttttgca aaaattgatg aaatcgcaga
     9301 atacaaccag ttaaaggtca tccacgccat gcaggcaagc aaggtcagcg aggcatgctt
     9361 atatggaacc acaggatacg gatacaatga cctgggacgt gatacgctgg agcaggtcta
     9421 tgcaacgtgc ttcggcactg aggatgcact ggtacgcccg cagatcgcct gcggaaccca
     9481 tgcgcttgca acggcgcttt ccggaaatct ccgcccgggc gacgaactgc tttctcctgt
     9541 tggaaaaccg tacgacaccc ttgaggaagt gatcggcatc cgcccgtccg tcggatctct
     9601 tgcggaatat ggcgtaactt accgccaggt agatttaaaa gaggacggct ctttcgatta
     9661 cgaaggaatc aaaaatgcca tcaacaaaaa gacaaagatg gtgacgattc agagatcgaa
     9721 aggatatcag accagaccga cgctttctgt tacccgcatc ggtgaactga ttgcatttat
     9781 taaaaatatc aaaccagatg tcatctgtat ggtcgataac tgctacggcg agttcgtgga
     9841 gacgatcgaa ccgaccaatg tcggtgcaga tctgatggtc ggatccctga tcaaaaaccc
     9901 gggcggcgga ctggctccgg ccggcggtta cattgttgga aagaaaaagt acgtggaaaa
     9961 tgcagcatac cgtctgctct ccccgggact tggaaaagag gttggcgcaa cactcggtgt
    10021 gaacggttcc ttctaccagg gattcttcct ggcaccgacc gttaccgcag cggcattaaa
    10081 aggtgctgtc tttgctgcaa atgttaatga gaaactggga tttgccgttg ttccaaacgg
    10141 aaccgagagc cgtcacgaca tcattcaggc ggtgaccttt ggaaaaccgg aaggcgtcat
    10201 cgcattctgc cagggtatcc aggcagctgc cccggtcgac agcttcgttt ccccggaacc
    10261 ttgggatatg ccaggctacg acagcccagt tatcatggca gctggcgctt tcgtgcaggg
    10321 ctcttccatt gaactttctg ccgacggccc gatcaaaccg ccgtacgctg tctatttcca
    10381 gggtgggctt acgtggcagc acgcaaaatt cggtattctg atgagtcttc agaaactcgt
    10441 cgatgccggt atggtgacat tataatggca aaaaatgtgg taattatcgg cggcggcgcg
    10501 agcggactga tggcggcaat ttgggcctca cgtctcggtg ccgcggtcac cgttctggaa
    10561 aagaacgata aaccgggcag aaagcttctt gcgaccggaa acggacggtg taattttacc
    10621 aatcgttatc aggatgcatc gtgctaccgc acgggaaatc aggagcgtgc atcacgggtt
    10681 ctcgaacagt ttaccgaaca ggacaccgaa cagttttttg aacagctcgg catccgcatc
    10741 cggagccgtg aaggctggct ttatccggcc agcgagcagg cccagtccgt tcttgatctg
    10801 ctggttttgg aagccagatt ccgaaaggta aaaatcaaga cacgggaaac ggctgttgca
    10861 gtggaaaaag ccgaaaatgg ttatctggtg cgcaccgaag gatggcagta cccggctgac
    10921 agcgtcatca tctgctgcgg aagctgtgct tcccagatcg ccggttccga cggagataca
    10981 cttcgatttg ccgatcagct tcagctcgct tcgattccgt tttccccggc cctctgcccg
    11041 cttcgctgca aaggaaacca gtttccctcc tgggcgggtg ttcgggtgcg cgcgaagatt
    11101 acgcttctgg ttgaaaatca ggagattctg acggaacagg gagaaatgca gcttaccgac
    11161 tacggtgtct ccggcatccc ggtattccag atttcccggt tcgctgtgcg cgcctgcatg
    11221 cagaaaaaac gcgctgaact tctcgtagac ttctttccgg aactgacgga atcggagctt
    11281 actgcagaat ttgtcagacg gcaggaaatc tgcccgtata aagcgcccaa agagcttctg
    11341 atcggtcttc ttccggaaaa actcattccg gttctgatcg gaaagaaaaa gactccggaa
    11401 gaaatggcag gcgctatcaa atcatatcgt ttacagatca ccggccagac cgactttgga
    11461 aaagcccagg tgtgtgcggg cggcatcacg ctcgaccagc ttacggattc tctggaatcg
    11521 gtacagcacc ctggtatttt ctttgcggga gaagccctgg acgtcgacgg cgtctgcggc
    11581 ggctacaatc tccagtgggc ctggtccagc ggaagtgcag ccggaagagc tgcagcagga
    11641 gaacatgcat gattcggata cagcaattaa agcttccagt cacccacaca caggaggaac
    11701 tggaacagaa aataagaaaa acactgaaaa tcggaaaaca cgatctgcag gaaattgagc
    11761 tcttacgccg ctcaattgat gccagaaaga aatccgaatt aaaatacgtt tatcagcttg
    11821 atgtaaaagt aaaagatgag aacgccgtac tgcggaaggc gaaaaataac cagatcgtaa
    11881 aagcggagaa aaagtcatat gtgttcccaa aatccggaga acagactctt gttcaccggc
    11941 cggttattat cggaagcggt ccggcagggc ttttttgtgc ctatgttctc gcaaagcatg
    12001 gctaccgtcc gctcgttctg gagcgcggcg aatctgcaga acagagaaag aaaagtgtcg
    12061 atgcgttctg gaaaaccggc gttctgaacc cggaatcgaa cgtacagttc ggagagggcg
    12121 gcgccggtac gttttcggat ggaaaattaa acacttcggt gaaggatcca tccggcagaa
    12181 accgtgaggt actccggatt ttcacggaat gcggggcacc ggaagaaatt ctgtacgatc
    12241 agaaaccgca ccttggcacc gatcagctca tcggaattgt gacaacgatg cggaaaaaga
    12301 tcgaggcatg gggcggagaa gtccgtttcg gagcaaaagt gacggacttt ggaattgaaa
    12361 acggcagact cacttctgta accgtcaatg aaaccgagaa gattgccgcg gagacggcag
    12421 tccttgcgat cggccactct gcacgcgata ccttctttaa actctgcgaa aataagatct
    12481 ccatggaagc aaaatccttc gctgtcggcg tccgcattga gcatccgcag aagatgatca
    12541 cggaggatca gtacggaccg gaggcaccgg attttcttgg ggcagcaccg tataagctca
    12601 ccaatcagtg tgaaaacgga agaggtgtgt actcattctg catgtgtccc ggcggctatg
    12661 ttgtcaacgc atcctcggaa gcggaacgca cctgcgtcaa cggtatgagt tattctgacc
    12721 gggaaggtaa aaatgcgaac agcgctatga ttgtcaccgt aacaccggaa gactaccgcc
    12781 cgtaccatgt agagggaacg ccggacgtac tcgatggtgt ggcattccag agagcgcttg
    12841 aacacgctgc gtgggaagcc ggaaaaggca aagttccggt gcagcttttt ggtgatttct
    12901 gtgaaaacag agtttctaca gcacttggag aggtaacccc ttccatctgc ggagaatgga
    12961 ctttcgccaa tctgcgggaa gttcttccga cgtttatcgg agactccctt gtggaaggca
    13021 ttcgtgcatc tgagcggaaa atccacggct tttcccgtcc ggacgccgtt ctttccggcg
    13081 tggaagcgcg tacctcgtca ccggtccgca ttgtaagaaa tgaaacgctc gaaagttcgt
    13141 cgcttaccgg tctttacccg tgcggagagg gcgcaggcta tgcgggaggc atcacttccg
    13201 cagccatgga cggattaaaa acggcagagg aaattgcaaa aaaatatatg aatttttcat
    13261 aattcctata catctgcgga aagttgtgct agaatgatgg gtgattcaaa ccaatgtatt
    13321 ccatgaaaat tcccggaaga gtggtgggag gaatacatga gaaagaatgg attacgaaac
    13381 ctgccgacga tggaacagcc atatgaaaaa tgcctggaaa acggcccgga aagtctttcc
    13441 gacgcggagc ttctggctgt cattttacga tgtggggcaa ggggaaaaac atccctggag
    13501 ctcgcaagag aaattctttc ccttcccgga aaagaggagg gcctgttagg actctatcac
    13561 tgtacaagac aggaacttct gcagatccgc ggagtcgggg aagtaaaagc gatccagctc
    13621 cagtgcatcg gtgagctttc caaacgaatg gccgcggccg gagcaagaaa aggtctctgc
    13681 tttcatgatc cggcttccat tgcggattat tacatggaac gtctccgcca cgaggatcag
    13741 gaggttcttc tctgcatgat gctggacacn nnnnnnnnna aaggaaatca caagaggaac
    13801 ggtcaatgcg acgatgattt cgccgcggga gctttttctt gcggcactgc agttccgtgc
    13861 ggttcatatt ctgctggttc acaaccatcc aagcggaatc ccggaaccaa gcggtgacga
    13921 cattaccgtg acacggcaga tccggcgggt gggcgaaatg ctcggcatta ccctgctgga
    13981 tcacattatt atcggggatc actgttatac cagtatgctg gaacagggaa tctgatcccg
    14041 aaaatccgag gcagaaatgc ccaaaggaag ggaacaagtt aaaggagcac gatgcagatt
    14101 cgaaatgtat acggcataga ccttggcacc agtaccgtca aaatctatga cctgaagaaa
    14161 gacacaatca caaaagaaaa aaatatgatt gccatacgca accgcgaaca ggtgattgcc
    14221 gttggaaatt acgcatatga aatgcacgag agaacgcctt ccaatatcga ggtaattacc
    14281 cccatgagca acggacgcat tgcgaatgta ttgatggtag aagccgtttt gcacacgctt
    14341 ctccaccgct gtacgcgcca catgggatac cgcccggagc tgtacttttc agttccctgc
    14401 gatatgacag agattgaacg gcgttcttat tatacgattg cacataaggg agccttaaaa
    14461 aactgccgca tgtatcttgt ggataaacca atcgcggatg cgctggcact tggcattccg
    14521 atcaaccgca cgaagggttc catgattgtc aacatcggag cgcagagcac agagatttct
    14581 gttatcgcca acgcgagggt tattttttcc agaatcatcc cggtaggcgg aaaacagttc
    14641 aacgaatcga tatgcaatct gaaccgccgc aaaaacaatt tccagattgg ctccaaaaca
    14701 gccaaacgtg tcaagattgc gctggcggat cttggaacag acaaaaaaga ggcgcggaag
    14761 gtgcgcggcg tagatggtgc atccggtctt ccaatggaag ggatcattac ttcttccctc
    14821 gtaaacgaag cactcctttc cggtgttaat gagatcggcg aggaaatcaa gcatgcgctg
    14881 gagcgtacac cgccgcagat ccacgatcat atccagaaag agggtattta catcaccggc
    14941 ggaagcacaa ggattccgaa cattgaccgc tatctttcga gacagctcgg ctgtccggtg
    15001 cagctttccc agtattatga cctctgtacc atctgtgggc tcaaggaact gatcacgcac
    15061 gatgcgctgc atcgctgggc ttacacggta aataaaaaga agtaaatcta tcaaaaagga
    15121 cccgctatga ataagaagaa aaaatttcag ctaaaaagca aacatttact tttcatcatg
    15181 acgttcctgt gtctctccgg cattttaggc agtgcagcag acaaaatgcc ggtcacgccg
    15241 atccgggaag ccgcaggctt tgtgatcatc ccgtttcaga acggaattaa ccagatcggt
    15301 gcgtatttga cagactgtct gaccggtttc cagaacgtca aaaaactgtc tgccgagaaa
    15361 gaggagcttg aacagaaggt cacggatctg actgaggaaa acaatcagct tgtacaggat
    15421 cagcaggagc tgcagcgtct tcaggagctg tatcagctgg accagtccta ctcggaatat
    15481 gaaaaagtag cagcacaggt catctcaaag gatccgggca actggtatga tacctttgtc
    15541 atcaacaaag gatcaaatga cgggattcag aaagatatga acgttattgc agacggcggc
    15601 cttgttggac ttgttgagga ggtaagttcc aattccgcta ctgtccgctc tatcattgat
    15661 gactccagtt ccgtgagcgc catgacagca tctacctcgg atacgtgtat tgtatcgggt
    15721 gatctgcgcc tgatcagcga cggaaagctt gccttttccc agctgaatac aaccgacacg
    15781 gtagctgagg gcgaaaagat tgtgacatcg aacatcagtg ataaatacct ccgcggtatt
    15841 ctgatcggct atgtcagtga aatcaccgag gactccaatc acctgacgaa aaagggatac
    15901 atcattcctg ttgtggattt tcaacatatt caggaagttc tcgtgatcaa agaattaaaa
    15961 cagcagggag gagagtaaat gcgcagaaaa atcacgcttg cctttctgat tctggcagct
    16021 ttcctgttac agacaactgt atttcagtat tttcagatcg cgtccgtaac gccgaacctt
    16081 cttctgattc tgacgtccgc ctttgggctg atgtacaaaa ccaaaaaagc gcgccgctga
    16141 gcctgcgcgg aaaaaaagag ggactgatgg tcggattttt cagcggtctg atgattgatc
    16201 tgttctacgg cagcctgttt ggattttacg cactgctcta catgtatatc ggttatctga
    16261 acggcttttt ctgcaaagtc ttctatgacg atgatattaa ggtacacatt gtactggtcg
    16321 ctgccagcga tcttgtctac agtctgctga tgtatgtgct gcagttcctg ctgcgcgtgc
    16381 gcctgcactt tttcgattat ctggcgcata ttattctgcc ggaaatggta tacacggtag
    16441 tgctctccat tctgctttac cggcttttat ttatcatcaa ccgtaaactg accgaaaatg
    16501 aaatggaagg acaacagtcg ccatggctaa gaagataaga aaatttttcc gcagactcgg
    16561 tgaaaaccgc accctattgc ttggtgtggt tttccttgct atgtttgccg tcctgatcgg
    16621 aagacttttt atcctgcagg tggtgcatgg agaagagtat gcggataatt ttgaactgca
    16681 aatcacaaaa accagaaccc ttgagagtac aagaggaaat atttatgacc ggaacggaaa
    16741 actgatcgct ggaaataagg tttcctactc cgtaaccatc gaggataacg gaacgtacaa
    16801 cacgacaaaa gaacgtattc tctctctgaa cgcagaactc tatcgtctct gtaagctgat
    16861 tcgtgcaaac ggagactcga ttgacaccag caccttcccg attgaagtgg atgagaacgg
    16921 tgcgtttgtt tttacaggtg aagagggaac gacccgcgac cgtttccgtg cagacattta
    16981 tggacagaag aaaatcgacg atatgacggc agaacagaag agttccagtg ccgaaacgct
    17041 gatgacattt cttgccggac cggacggctt tggcctggat gcctattctg acgatgaaaa
    17101 atacgcatat tctgccaaag attttgaaga gtatggtctt ccataccaga cagacgaatc
    17161 cggaaatgcg gtcctttctc tttccaatca ggagcgtctg gaaattctgg tgatccgcta
    17221 taagatgaaa cagacgaatt atcagaagta tgtccgcgtg acagttgctt ccggcgtcag
    17281 cagcaataca actgcatcga ttgcagagaa tgaagatgtt ctgcagggtg tcagcatttc
    17341 ggaagattcc gagcgtgtat attatgacgg aaagtatttt tcctctctga tcggttatac
    17401 gggccaggcg tcctcagacg agctgacaga actgaatgaa gagctgaaga gccagggaaa
    17461 agaagaaacg tattccacgg aatccatcgt cggaaaatct ggtctggaac agtatatgga
    17521 gacaatcctt cagggaaccg acggatcaga agagattatc gttaataacg tcggaaaaga
    17581 gctggaaacg gtagagggat ccctggttga gccaaaacag ggaaataacg tctatctttc
    17641 gattgattat gacctgcaga ttgcggtata taaaattctg gaacagagaa ttgccggcat
    17701 tctgaaacag tatctgagtg atgtaaaatc cgttgatacc tcaacacttg ccaatacgga
    17761 tgccgttccg attccaattt acgatgtata caatgcactg atcgaaaaca gcacgattga
    17821 tatttcccat ttctcggcgg cggatgccac ggagcgtgag cagcggatct atgcactgtt
    17881 ccagcagaag ctccagcagg tgcttgccga gattacgcag gaactgaccg ttgaaacagc
    17941 aaccgtttac aacgacctca gcgatgaaat gcaggaatat gagaccttca tcgtggataa
    18001 gctgctttcc agtacgatgg gtattctttc ttcaacagca attgatgaga aagacgccac
    18061 ctatcaggca tggaatgacg gaacgatttc tcttcgtgat tacctgacgt atgcggcaag
    18121 ccagaactgg attgatgtct cagcactgac gcaggatgat gcctacctgg attctcagga
    18181 ggtttatcag aagctgacgg aggttattct ggaccgtctg gcaaacaata caacatttgc
    18241 aaaaaagatg tatcactata tgctgctgca ggatatgctc gatggctatg atatctgcaa
    18301 tctgatgtat gatcagaacc ttctgacgaa agaggatgac gattatgccg cttatgttgc
    18361 aggaaatatg acaccgttcc agcttctgtc cgataaaatc gccaaactgg aaatcacccc
    18421 ggcacagctt gcactcgatc cgtgttccgg ttctgctgtc attacggacc cgaataccgg
    18481 tgctatttta gcctgtgttt cctatcctgg ctacgacaac aaccgcctgg caaaccagat
    18541 ggataccgca tactgggcaa aattgaatac cgatgaatcc agcccgctgt acaacaaagc
    18601 aacccagcag aagacggctc ctggttccac ctttaaacca ctgatggcgg ttgccggatt
    18661 aagcgaaggc ataattactc cgaccagtac catcaactgc aacggtctgt tcggggaagg
    18721 acttgtcaac gaatctgact atgtccactg ccatcagttg agcggccacg gagatctgaa
    18781 cattgtcggt gccatccaga actcctgcaa cgtgttcttc tgtacgcttg gatatcgtct
    18841 gggactcgat gagaacggca cctttacaca gaagcgctct ctggaaatga tccagaaata
    18901 tg
//
LOCUS       DAOMUF010000019        18865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_521_length_18865_cov_3.801914, whole genome shotgun sequence.
ACCESSION   DAOMUF010000019 DAOMUF010000000
VERSION     DAOMUF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 18865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18865
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_521_length_18865_cov_3.801914"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PLN77_02390"
     CDS             <1..927
                     /locus_tag="PLN77_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM43164.1"
                     /translation="QQLKENIAKVMIGNERTIELVLTCLVAKGHILLEDVPGTGKTVM
                     AKSLAKSVEAEFGRIQFTPDLLPSDVTGLNYFDAKSGEFVFSKGPAFCNILLADEINR
                     ATPKTQSSLLECMAERQITVDGVTRPLEEPFLVIATQNPLETLGTFPLPEAQMDRFLM
                     KLSMEALSPAEETQMIARFLTEEPLAELSSVCQKEEIRALQEACKEVYLHPELVQYLV
                     RIVQETRVNSKIASGVSPRGTLAFLRAVQGHALVQGRNYVVPEDFKTVAVPVLAHRLT
                     MQIGADDGRAAESVIEEILNRIDLPTENWSGR"
     gene            934..2064
                     /locus_tag="PLN77_02395"
     CDS             934..2064
                     /locus_tag="PLN77_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRM43165.1"
                     /translation="MLLLLLFGMALAWFLQKKLYQKNWQKNLKIQVSFQDSAIYEGEE
                     STLKEVVVNDKLLPIPALELGISMSRNLEFDREAKANASITDQSYKRDVFSFLFHQQV
                     TRTLPFQAKKRGFYEITGAHAVAYDLFFREGLYSDYPQQTQMYVYPRQVDTARIQMIC
                     RAVSGMVLSRNRLYEDPFEFSGIRDYRKEDPMNRINWKSSARMGDLMVNQFDATTSVE
                     VTVLLDIEDANILREEAQVEESIRITSSLAARMVKNKMNLWVKSNAKDEETGEELRVH
                     LAAGAGKTAELNRKLACVQLGGNLTGSAEFLHKEAEKELTGHTYVVVSKNRTDEVREA
                     LHLLSGAQNQVLWVIPYLPGEEVPSEQNRAYTAIGWEVKPWK"
     gene            2055..3434
                     /locus_tag="PLN77_02400"
     CDS             2055..3434
                     /locus_tag="PLN77_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43166.1"
                     /translation="MEIKKTPVQIPLTWLHVWLLFAGIYGFAAGFLELTGREALYFCA
                     ISLLLLIPAAASWILIRKTKALWQFLLGGIAVCAVTYLAAKFFCSLFSVQAAAVIEAA
                     AAANDGKNKFENGISPAFLESLSGVMTGLMAAVIFLIRGYVRIRKGQLKKAAQELPAG
                     SMPLADMEAWEIPTLLDEPKPLHFLWFMIQYVIGVLMKMPFYWHLIYWFFFADVFLCF
                     FCQYLDGMHGFIRDHQKIANLPVETMQRTGKLILKIAVLLLVLFVLPSALYGKDPIAE
                     AIAGYEPKELESDLTIETPEAETPQGMEQADLSDMLGDKEYKPMPQWIQNLFTAVMYL
                     VMVAVGIAVLRAIYQSVKNAGKAFSEDEKDEVLFLHQGNDERENLPWRREKKEGYLSP
                     NMRIRRQYKKTIKKAGKYQPTGAETPAELEERNELSGDGMRRLHDGYEKARYSCEGCT
                     KEEAEALRN"
     gene            complement(3436..3909)
                     /gene="spoVAC"
                     /locus_tag="PLN77_02405"
     CDS             complement(3436..3909)
                     /gene="spoVAC"
                     /locus_tag="PLN77_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HRM43167.1"
                     /translation="MQSLKSERAKKEYEQFVKEVTPKQNLFCNMAKAFIVGGLICVVG
                     QILLHIGKTQFSLSKDDAGSWCSLILILSSVILTGLNIYQKIVTFAGCGALVPITGFA
                     NSVAAPAIEYKKEGQVFGIGAKIFTIAGPVILYGVFASWLLGFLYWLWTAAGNWF"
     gene            complement(3929..4351)
                     /locus_tag="PLN77_02410"
     CDS             complement(3929..4351)
                     /locus_tag="PLN77_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HRM43168.1"
                     /translation="MRILPEILLAMAGLSLGFFIASGLVALVIGLGIVTRYAGITKTA
                     GSLRFYECCCMAGALFGDLFSLGTFSFSLPSWTAGVFWLFAGIYLGSWIIALGEVVNL
                     FSILCRRIGLTRGLPFVILCMAAGKIAGSLYYFASGFQ"
     gene            complement(4308..4976)
                     /locus_tag="PLN77_02415"
     CDS             complement(4308..4976)
                     /locus_tag="PLN77_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HRM43169.1"
                     /translation="MSEMLYLQTERNIKITSPVVTLGDVAQLSCSDGAVLARNQVRRV
                     AHLPDDGYGRYVCSASTLVRLVTEAEEHVDVTHIGEPNFILTYEDPRQKNTWISWCKT
                     ILVGILTFIGTAFSIMTFHTDIGVRELFLDVYTRFLPQMTPGFSVLEISYSIGIGLGV
                     VLYFNHFGRRKFTQDPTPLEVEMRVYEDDIDTTIIEQNDRSGKGKEAFHANPARDPAG
                     HGRS"
     gene            5134..6153
                     /gene="spoVAD"
                     /locus_tag="PLN77_02420"
     CDS             5134..6153
                     /gene="spoVAD"
                     /locus_tag="PLN77_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HRM43170.1"
                     /translation="MRQMTGKQSISFAKAVYIEGSAAIVGEKEKDGPLGEYFSHTLSD
                     PMCGQESWEEGESELQLATAKLAMQKANVRPEQIRMIFAGDLLAQSIASSFGLVDLNC
                     PLYGLFGACSTMGEALSLGAMAVAGGYGDRVLAVTSSHFGTAEKEFRFPLAYGNQRPP
                     SASWTVTGSGACVLGKTKSRVKITGATTGKIIDYGLKDNQNMGACMAPAACDTIAQNL
                     VDFNREPADYDRIVTGDLGVVGREILLELLKKKGYDISEQHSDCGMLIFDPAKQDTGA
                     GGSGCGCAASVLAEYLLPKLENGEWKRILFVPTGALMSKVSFQEGSSVPGIAQAVVIE
                     REEDV"
     gene            6167..6523
                     /locus_tag="PLN77_02425"
     CDS             6167..6523
                     /locus_tag="PLN77_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HRM43171.1"
                     /translation="MEYFNAFWVGGLICALCQILLDRTKMLPGRIMVTLVCTGAVLSF
                     LGLYDPFAEYAGAGASVPLLGFGHLLFQGVKDAVKESGFLGLFQGGLKAGAVGTSAAL
                     IFAYLASWIFQPKMKK"
     gene            6681..7343
                     /locus_tag="PLN77_02430"
     CDS             6681..7343
                     /locus_tag="PLN77_02430"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRM43172.1"
                     /translation="MKTREEALSYGLSFPDTYQEAPFHDDNWQLIRVKGSKKAFLWVY
                     EKDGIIQLNVKANPEWRDYWRDAFASVIPGYHQNKEHWNTILLDGTVPDDAVRTMIAE
                     SYDIITDSPTKRIYEAVKQIPRGKVATYGQIAALAGEPKMARAVGNALHKNTDPEHIP
                     CYRVVNSKGELAAEFVFGGAGKQADMLEADGIVLENGRVNLKKYGINVEEMLAQRELE
                     QN"
     gene            complement(7443..8645)
                     /locus_tag="PLN77_02435"
     CDS             complement(7443..8645)
                     /locus_tag="PLN77_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM43173.1"
                     /translation="MKYDFTSLMNRSGHDSIAVDQIPIPGAEVKEGFSRIPMWVADMN
                     FPSLPTIQEAIHARVNEPHFGYFDLPDAYFDNIIRWQKERNGVEDLPREAIGYENGVL
                     GCVASAAAAFTSPGESILLHSPTYIGFTGVLENCGRNIVLSDLVQDENGVWRIDYADM
                     DRKLKEHNIHFAIFCSPHNPTGRVWEREEIEKAMEIYKANECVVISDEIWSDLTLPGF
                     KHIPTQSVSKDARERTIALYAPSKTFNLAGLVGSYHIIYNRMLRDRVEKASSLSHYNS
                     PNVLSVHALIGAYRPEGYQWVDELREVLKSNADYAYNYILEHFKGVKLSKPEGTYMLY
                     LDCEEWCKEHDTDMDTLLKAGVAVGVIWQDGRPFHRPYAIRLNLALPHVLVKEAFDRM
                     DKYVFNAK"
     gene            complement(8821..9903)
                     /gene="proB"
                     /locus_tag="PLN77_02440"
     CDS             complement(8821..9903)
                     /gene="proB"
                     /locus_tag="PLN77_02440"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRM43174.1"
                     /translation="MMEKQTIVVKVGTSSLTLSDGTMDAEKIRNITTQLAALQTAGHH
                     VILVSSGAIAAGFSRLGFKKRPTKIADKQAAAAVGQGLLMEEYTKNLMAYQIPCAQIL
                     LNRSDFEDRRRYKNIFGSLSVLLGRGAIPIINENDTIAIEELKVGDNDTLSAQVAAML
                     HASLLILLTDIDGLYTANPKSDPNARHIDVVNEITPELTAAAGGAGSGNGTGGMTTKL
                     SAASLATRAGVPVLICSSAEENNIVRAVKGTAKGTYFTASGHNMKTRLQWMAFYAPSA
                     GNLYVDEGAAEALAVQAKSLLPKGIVAVEGDFKKGDVVSVFRRGSHEYLGKGIVNYDR
                     PDLQKIMNESTSHTEAINRDNWIGAE"
     gene            10182..11294
                     /locus_tag="PLN77_02445"
     CDS             10182..11294
                     /locus_tag="PLN77_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRM43175.1"
                     /translation="MEEKTEERGRGCMTTLCYIEKDDSYLMMHRTVKKNDVNKDKWIG
                     VGGHAELGESPEECLLREVKEETGYTLTSWRFRGLVTFVTEAENSKTVEYMEYMCLYT
                     ADGFTGEPTACDEGELAWVKKEDVLHLNLWEGDKIFFRLLNEDEPFFSLKLRYVGDTL
                     AEAVLNGKQMELFEERSGDGTPTGTIVERGVAHSEGRCHGTAHIWIARANEKSGCEVL
                     LQKRSAWKDSNPGCYDISSAGHLSAGDTYLEGALREIGEELGIHAEAEELRDLGLLEK
                     VSHGVFYGKPFHDHEVSAVYLYTKPVEAEKLHLQESEVEAVRWMDLKECQKAVREGSI
                     PNCIDIRELEMIEKAWFAGEKTKDEAEKEAAATAEK"
     gene            complement(11377..11970)
                     /locus_tag="PLN77_02450"
     CDS             complement(11377..11970)
                     /locus_tag="PLN77_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM43176.1"
                     /translation="MKNEKVWKIAMTGVFAALSYVVFTFLQFKIWLPGGDATSIHFGN
                     AVCVLGALILGGVYGGIGGAIGMTIGDLLDPVYVVYAPKTFILKFCIGLIAGLIAHKI
                     GKINEQTDQKQIFKWALLAAIGGLLFNVIFDPLIGYFYKLVLLGKPAAEVTLSWNVAA
                     TSINAVLSTIASVVIYMALRPALQKSGLFSQVKAAAK"
     gene            complement(12081..12461)
                     /locus_tag="PLN77_02455"
     CDS             complement(12081..12461)
                     /locus_tag="PLN77_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HRM43177.1"
                     /translation="METVLLYQIKGTKTAALLKPVLLKLGIRVRIVEPEQYLQSIGFL
                     AGNKAFAESPEAYDGAGFDEPMMVMAGFSERKLDLFLTEMRRKKVPPIALKAIVTTQN
                     QAWNSLQLYRELKEEHEKMKSYRK"
     gene            12625..13491
                     /locus_tag="PLN77_02460"
     CDS             12625..13491
                     /locus_tag="PLN77_02460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM43178.1"
                     /translation="MWIADGWKDYEVLDTSGGEKLERWGDYLLVRPDPQVIWNTKKEN
                     PGWKKKNGHYHRSSKGGGEWEFFHLPEQWEINYKDLTFRLKPFSFKHTGLFPEQATNW
                     DWFGDLIRRANRPIKVLNLFAYTGGATLAAAAAGASVTHVDASKGMVNWAKENAAVSG
                     LSDKPIRWLVDDCTKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEESIHPFIRL
                     CTQLLSDDPLFFLVNSYTTGLAPAVLTYMISLELKQYKGHVEAQEIGLPVKSTGLVLP
                     CGASGRWQSDNV"
     gene            13709..14995
                     /locus_tag="PLN77_02465"
     CDS             13709..14995
                     /locus_tag="PLN77_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRM43179.1"
                     /translation="MKPYIEWTKEELVAEKARLEQEFAEVKAKGLNLNISRGKPAAAQ
                     LDLTEGMLTVLSKNEDTFAEDGTDCRNYGVLTGIPEARKLIGDICEVPAENVIVYGNA
                     SLTIMFDTVARSFLKGVAGCTPWNKLDKVKFLCPVPGYDRHFGVTEYFGVEMINVPMT
                     PEGPDMDMVEKLVAEDDAIKGIWCVPKYSNPQGYTYSDETVRRFANLKPAAKDFRIFW
                     DNAYIIHHLYDDRQDHLLNIIEECEKAGNPDMVYEFVSTSKVSYPGAGIAALISSEAN
                     LKEAQEYMNFQIIGHDKLNQLRHVKFFQNLDGVMNQMKKHAEILRPKFEAILQVMDQE
                     LSGLGIASWTKPNGGYFISFDAMEGCAKKIVAKAKEAGLTMTGAGATYPYHNDPQDSN
                     IRIAPSFPTPEELEIAGQVFVLSVKLVSVDKILESK"
     gene            15126..16058
                     /locus_tag="PLN77_02470"
     CDS             15126..16058
                     /locus_tag="PLN77_02470"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HRM43180.1"
                     /translation="MKKKVIGIAAGLILLAAAGGYAGVGHYYASHFFPKTAINGLNCA
                     GLTAEQVKEEIQKHIVDYTLSITERGGKTETLSGTEIGLTYVDDHAVEKLLESQNTLA
                     WPAFYWKEKENQVAADSVYDKEMVQEKLQTMEGFQEEQQEAPTDAYLTDDGTSYVIVP
                     ETEGEQVDYEKAEQAVIEALDAGAASVDLEEKDVYRKPGITQDDEALNGEMAELNHLT
                     AARITYAIGENSYAIDRATLQSWLVQGEDGTYTISQDEAAAFVRHMAYETDTFGLAHT
                     FKTSLGATINLNAGGDYGWCIDKEETTQALLQAI"
     gene            16146..16544
                     /locus_tag="PLN77_02475"
     CDS             16146..16544
                     /locus_tag="PLN77_02475"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRM43181.1"
                     /translation="MCISQQKMWCYKDGVLVTETPVTTGNHATGYDTPAGSVWAVDAK
                     KSDCDFKLYPSHVMFWLPFNGDVGIHDASWRTEYGGDIYLTNGSHGCVNTPYTEAEKV
                     FNAIEIGDPVIVYYSLDDVTGPQPTQKNSL"
     gene            16600..17649
                     /locus_tag="PLN77_02480"
     CDS             16600..17649
                     /locus_tag="PLN77_02480"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRM43182.1"
                     /translation="MKIVVLAGGTSTERSVSITSGTMVCKALREKNHQAVLVDVFCGI
                     DVKGNLEDVFAEEAYDIDKAAEYMHSFDAGLNEMIESRRRFFGPNVLELCEAADVVFL
                     ALHGANGEDGKVQATFDLLGIPYTGTGYLSSALAMDKSITKQFFRAHHVPTPNGVTLK
                     KGQESEPLSGFGLELPVVVKPCCGGSSVGVYIAHTNEEYWQAMKDAFSYEPEVVVEEY
                     IEGREFSVGVVDGKAYPIIEIAPIEGFYDYTNKYKAGATVDTCPAVLTEEQTKEMQKH
                     AEDGAAALGIENYCRLDFMMRKDGRMYCLEANTLPGMTPTSLLPQEAQVLGISYPELC
                     ELLIQLSLRKRGNNA"
     gene            17646..>18865
                     /gene="murF"
                     /locus_tag="PLN77_02485"
     CDS             17646..>18865
                     /gene="murF"
                     /locus_tag="PLN77_02485"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRM43183.1"
                     /translation="MKNMTLAHITEVCGGIYFGPEEKKTAEVTDIVTDSRKAGEGSLF
                     VAIPGERVDGHKFIPNVASQGALAVISEQKLETPPCPYILVKDSMEAIKAMAEYYMQQ
                     LNIPVVGITGSVGKTSTKETIASVLAQKYRVLKTDANFNNELGLSLTVFRLREEDEMA
                     VLEMGIDDFGQMHRLAKIARPETAVITNIGWCHLENLKTRDGILQAKTEIFDHLRENG
                     HIIINGDDDKLSTVGTVHGIKPVHFGLDEKNEYYADEIESQGFRGISCRIHTPHGSFS
                     ALIPIPGRHMVYNALAGTAVGCTYGLTLEQIKQGIETLQSVSGRFHIIETGKYTVVDD
                     CYNANPVSMKASLDVLKEAKGRKVAVLGDMGELGTDEAALHAEVGTHAGTCGIDALYC
                     AGPLCEHLAKAAKEA"
BASE COUNT         5024 a         4550 c         5336 g         3955 t
ORIGIN      
        1 cagcaattaa aagaaaatat cgcaaaggtc atgatcggaa acgagcgcac gattgagctt
       61 gtcctgacct gccttgttgc caaaggtcat atcctgctcg aggatgtccc gggaaccgga
      121 aaaacggtca tggcgaaatc ccttgcgaaa tccgtcgagg cagagttcgg ccgcatccag
      181 ttcacaccgg acttactgcc gtccgatgtc accggtctca attattttga tgccaaaagc
      241 ggagagttcg tcttctcgaa aggccctgcc ttctgcaaca ttctgcttgc cgatgagatc
      301 aaccgtgcaa cgccgaagac gcagagcagc cttctcgagt gcatggctga gcgccagatc
      361 accgtggacg gtgtgacacg gccgctcgag gagccgttcc tcgtcatcgc cacgcagaac
      421 ccgctcgaga cactcggaac cttcccgctg ccggaggcgc agatggaccg ttttttaatg
      481 aaactttcga tggaagcatt aagcccggcg gaagagacgc agatgatcgc ccgtttcctg
      541 accgaggagc cgctcgccga gctgtcgtcc gtctgccaaa aagaagaaat ccgtgccctg
      601 caggaggcgt gtaaagaagt ctacctgcat ccggaactgg tacagtatct cgtccgcatc
      661 gtacaggaga cgagagtcaa cagcaaaatt gcaagcggtg tgagcccgcg tggaacactc
      721 gcatttttac gtgccgtaca gggccacgcg ctggtgcagg gacgaaacta cgttgtgccg
      781 gaggacttca agaccgtggc tgttccggta ctcgcacacc gtctcaccat gcagatcggc
      841 gcagacgacg gaagagccgc agaatcggtg atcgaggaaa ttttaaaccg catcgatctg
      901 ccgaccgaaa actggtcagg aaggtaacgg actatgcttc ttttattact atttggaatg
      961 gcgctcgcct ggtttctgca gaaaaagctg taccagaaaa actggcagaa aaatctgaaa
     1021 atccaggtca gcttccagga ttccgcgatc tatgagggcg aggagagtac gttaaaagag
     1081 gtcgtcgtaa acgacaaact gctgccgatt ccggcactcg agcttggcat ttcgatgagc
     1141 cgcaatctgg aattcgaccg cgaggcaaaa gcaaacgcga gcatcaccga ccagagctac
     1201 aaacgcgatg tcttttcgtt cctgtttcat cagcaggtca cgagaacact gccgtttcag
     1261 gcgaaaaaac gaggattcta cgagatcacc ggtgcgcatg ccgtagcgta cgatcttttt
     1321 ttccgggagg gtctttacag cgattacccg cagcagacac agatgtacgt ttacccgcgc
     1381 caggtcgata ctgcacggat tcagatgatc tgccgtgccg tgtcgggcat ggttctgtca
     1441 cggaatcgcc tgtacgagga cccgtttgaa ttttccggta tccgcgatta ccggaaagaa
     1501 gacccgatga accgcatcaa ctggaaatcg tcggcacgga tgggagatct gatggtcaac
     1561 cagtttgacg cgacgacgag cgtggaagtt acggtgcttc tcgatatcga ggacgccaac
     1621 attctgcgcg aggaagcgca ggtcgaggag agcatccgca tcacgtcttc gcttgcggcg
     1681 cggatggtga aaaacaagat gaatctgtgg gtgaagagca atgcgaaaga tgaggagacc
     1741 ggcgaggagc tgcgtgtcca tctggcagcg ggcgccggaa aaacagcgga gctgaaccgg
     1801 aaactggcgt gcgtgcagct tgggggcaat ctcacaggca gcgcggagtt tctgcacaaa
     1861 gaggcggaaa aagagctcac cggccacacc tacgttgtcg tatccaaaaa cagaaccgat
     1921 gaggtccgtg aggcgcttca tctgctctcc ggtgcgcaga accaggtgct gtgggtgatt
     1981 ccgtatctgc cgggcgaaga agttccgtcg gagcagaacc gtgcgtacac cgcaatcggc
     2041 tgggaggtga aaccatggaa ataaaaaaga caccggtaca gatcccgctg acctggcttc
     2101 atgtctggct tttattcgcc ggcatctatg gctttgccgc tggttttctg gagctgacgg
     2161 gaagggaggc gctgtatttc tgcgccatat ccttattgct gctgattccg gccgccgcaa
     2221 gctggattct gatccggaaa acgaaagcac tctggcagtt tctgcttggc ggaattgcgg
     2281 tctgcgcggt gacgtatctg gcagcgaaat ttttctgttc cctgttcagt gtgcaggcgg
     2341 cagcggtaat tgaagcggct gcggcggcaa acgatggaaa aaacaagttt gagaacggta
     2401 tttctcctgc atttctggag agcctttccg gcgtgatgac gggtcttatg gccgctgtga
     2461 ttttcctgat ccgcggctat gtcagaatcc gcaaaggcca gttaaagaaa gcagcgcagg
     2521 aactgccggc cggctcaatg ccgctggcgg atatggaagc gtgggagatc ccgacgctcc
     2581 tcgatgagcc aaagcccctt cattttctct ggtttatgat acagtatgtg atcggtgtgc
     2641 tgatgaaaat gccgttctac tggcatctga tctactggtt tttctttgca gatgtattcc
     2701 tgtgcttctt ctgccagtac ctcgacggca tgcacgggtt catccgtgac catcagaaaa
     2761 tcgccaatct cccggtggag acgatgcagc ggacgggaaa actgatcctg aaaattgcag
     2821 tactccttct ggtactgttc gtgctcccat cggcgctcta cggaaaggat ccgattgcgg
     2881 aggcgatcgc aggttatgag ccgaaagagc tggaaagcga tcttacgatc gaaacaccgg
     2941 aggccgagac gccgcagggc atggagcagg ccgatctgtc cgatatgctg ggggacaaag
     3001 agtacaaacc gatgccgcag tggatccaga atctttttac ggcggtcatg tatcttgtga
     3061 tggttgccgt cggaatcgcg gttctgcggg cgatttacca gagcgtaaaa aatgcgggaa
     3121 aagccttctc agaggatgaa aaggacgaag ttctcttcct gcatcaggga aacgacgaac
     3181 gggaaaatct gccgtggcgg cgggaaaaga aagagggcta tctctccccg aatatgcgta
     3241 tccgccggca gtataaaaaa acgatcaaaa aagccggaaa ataccagccg accggagccg
     3301 aaaccccggc ggagctggaa gagagaaacg agctttccgg cgacggcatg agacggctgc
     3361 acgacggcta tgaaaaagcc cgttacagct gcgagggctg cacaaaagaa gaggcggaag
     3421 cgctgcggaa ctgatttaaa accagttccc ggcagcggtc cacagccaat agagaaagcc
     3481 gagcagccag cttgcaaaga caccgtacag aatgacaggt ccggcgatgg tgaaaatttt
     3541 cgcaccgatg ccgaagacct gtccttcttt tttatattca atcgcaggag ctgcaaccga
     3601 gttggcaaag ccggtgatcg ggacgagcgc accgcagccg gcaaaggtga cgattttctg
     3661 atagatgttg aggccggtta aaatcacact gctaaggatc agaatcagcg agcaccagga
     3721 gccggcgtca tctttgctga gcgagaactg cgttttgccg atatggagca gaatctgccc
     3781 cacgacacag atcagaccgc cgacgataaa ggcttttgcc atattgcaga agagattctg
     3841 ctttggcgtc acctctttga caaactgttc gtattctttt tttgcccgtt cactttttaa
     3901 actctgcatc acatcttacc tccagttctt attgaaatcc acttgcaaaa taatagagag
     3961 agcccgcgat tttccctgcc gccatgcaga gaattacaaa cggcagtccg cgcgtcagtc
     4021 cgatccggcg gcagaggatc gaaaacaggt tgacgacttc gccgagcgcg atgatccagc
     4081 ttcccagata aatgcccgca aaaagccaga acacaccggc cgtccaggag ggcagggaaa
     4141 acgagaaggt gccaagcgag aaaagatcgc cgaacagcgc cccggccata cagcagcatt
     4201 cgtaaaaacg caggctccct gctgtctttg tgatgccggc gtaccgtgtc acgatgccaa
     4261 ggccgatcac aagcgcgaca aggccgctcg cgatgaaaaa gccaaggcta agaccggcca
     4321 tggccagcag gatctctggc aggattcgca tgaaaagcct cctttccttt accggagcgg
     4381 tcgttctgtt cgataatggt cgtatcgata tcatcctcgt agacccgcat ttccacctcg
     4441 agcggcgtcg ggtcctgcgt gaatttccgc cgtccgaaat ggttgaaata gagcacgacg
     4501 ccaaggccga tgccgatgga atatgaaatt tccagcaccg aaaatccggg cgtcatctgc
     4561 gggagaaaac gggtgtaaac atccaaaaac agctcgcgga cgccgatgtc ggtgtggaag
     4621 gtcataatgg aaaacgcagt tccgataaac gtaaggatgc cgaccagaat cgttttgcac
     4681 cagctgatcc aggtgttttt ctgacgcgga tcctcgtacg ttaaaataaa attcggctcg
     4741 ccgatgtggg tgacatcgac gtgctcctcg gcctccgtga cgaggcggac gagggtggag
     4801 gcggagcaga cgtagcgccc gtatccgtca tcgggaagat gcgccacgcg gcggacctga
     4861 ttgcgggcga ggacagcgcc gtctgaacag gaaagctgcg ccacatcgcc gagcgtcacg
     4921 acgggactgg tgattttgat attccgctcc gtctgaagat acagcatttc actcatgaaa
     4981 aagattcctc taaaagtttc tgaaacagga tttttgtgct acagcgtaca gagttcccta
     5041 aaacaggaaa atcatgcgta agaatggaaa aaacggaaat actgaaggtc agaaatggta
     5101 agacgcaaaa taagaaaacg gaggaatttg ccgatgagac agatgacagg aaagcagagc
     5161 atctcgtttg ccaaagccgt ctatatcgag ggcagcgcgg cgattgtagg agaaaaggaa
     5221 aaggatggac cgctgggcga atacttttcg cacacgcttt cggacccgat gtgcgggcag
     5281 gagagctggg aggagggcga gagtgaactg cagcttgcca ccgcgaagct ggcaatgcag
     5341 aaagccaatg tgaggccgga gcagatccgc atgatttttg cgggcgatct tctggcacag
     5401 tcaatcgcat catcgttcgg cctggtggat ctgaactgcc cgctctacgg cctgttcggt
     5461 gcctgctcga cgatgggaga ggcgctttcg cttggcgcga tggcagtcgc gggaggatac
     5521 ggtgaccggg tgctggcggt gacatcgagc cactttggaa cggcggagaa ggagttccgt
     5581 tttccgctcg cctacggaaa ccagcgcccg ccgtccgcga gctggacggt gacgggaagc
     5641 ggcgcgtgtg tgcttggaaa aacgaagagc agagtgaaaa tcacgggcgc tacgacggga
     5701 aaaatcattg attacgggct gaaagacaac cagaacatgg gcgcctgcat ggcccccgct
     5761 gcgtgcgata cgattgcaca gaacctcgtc gatttcaacc gggaaccggc cgattacgac
     5821 cgcattgtga cgggagatct cggcgttgtg gggcgggaaa ttctgctgga gctgctgaag
     5881 aaaaaaggat acgatatctc ggaacagcac agtgactgcg gcatgctgat cttcgatccg
     5941 gcaaaacagg acaccggcgc gggcggaagc ggctgcggct gtgcggcatc ggtgctggcg
     6001 gagtatctgc tgccgaagct ggaaaacgga gagtggaaga ggattctgtt tgtaccgact
     6061 ggtgcgctga tgtcgaaggt cagctttcag gagggcagca gcgtgcctgg tattgcacag
     6121 gcggttgtaa tcgagcggga ggaggatgtg taaaatggga acgttcatgg aatatttcaa
     6181 tgccttctgg gtcggcggcc tcatttgtgc cctgtgccag attctgctcg accgcacgaa
     6241 aatgctgccg ggaagaatta tggtaacgct tgtgtgcacg ggcgcggtgt tgagctttct
     6301 gggactctac gatccatttg cagaatacgc cggggcaggc gcgtcggtgc cgctgctcgg
     6361 gttcgggcat cttttgtttc agggagtaaa agatgccgta aaagaaagcg gatttttagg
     6421 attgttccag ggcggattaa aggcgggggc tgtcggaaca tcagcggcgt tgattttcgc
     6481 gtatcttgca agctggattt tccagccgaa gatgaaaaaa tagcaggtga aaaattgcgc
     6541 agaagcaggc ggactgatat aataaagaaa aatgccgcag aaaaaagcag aatcgcagcg
     6601 gcaggacgaa tacagaaaaa agcgggtgtt ttgcggaaaa aacatcaaaa cacccgcgac
     6661 aggaagaaag gaagtctgca atgaagacaa gagaagaagc gctatcctac ggcctttcct
     6721 tcccggacac atatcaggaa gcgccgttcc acgatgacaa ctggcagctc atccgcgtga
     6781 aaggaagcaa aaaggcattt ctgtgggtct atgaaaagga cggaatcatt cagctgaatg
     6841 taaaagcaaa cccggaatgg cgggactact ggcgcgatgc attcgcgtca gttatcccgg
     6901 gatatcatca gaacaaagaa cattggaaca cgattctcct cgacggaacc gtacccgatg
     6961 acgctgtgcg taccatgatc gccgagagct acgacatcat cacggacagc ccgaccaaaa
     7021 gaatctatga ggcggtaaag cagattccgc gcggaaaagt ggcgacatac ggccagatag
     7081 cagcgcttgc aggcgagccg aagatggcgc gtgccgtggg aaacgcctta cataaaaaca
     7141 cagatccgga gcacattcca tgctaccgtg tggtaaactc caaaggcgaa ctggccgcgg
     7201 aatttgtctt cggcggcgcc gggaaacagg cggatatgct ggaggcagac ggtattgtgc
     7261 tggaaaacgg ccgcgtcaat ttaaagaagt acggcattaa cgtggaagag atgctggcac
     7321 agcgggagct ggagcaaaac tgaaaaaaca ggatgcacaa aacagcgaaa gaccggaacg
     7381 gcagaggaaa atgtccgttt cggtctttca ttatgtttgg cttataaaat tagaaaatac
     7441 aattatttcg cattgaatac atacttatcc atacggtcaa atgcctcttt cacaagcaca
     7501 tgcggcagtg ccagattcag gcggatcgca tacggtctgt ggaacggccg tccgtcctgc
     7561 cagatcacgc cgacagcaac accggctttc agcagtgtat ccatatcggt atcatgctcc
     7621 ttgcaccact cctcgcagtc gaggtacagc atgtacgttc cttctggttt ggaaagcttc
     7681 acgcccttga aatgctcgag aatgtagttg tacgcataat ccgcattcga tttcagcact
     7741 tcgcgcagtt catccaccca ctggtatccc tccggccggt aagcgccgat cagcgcatga
     7801 acagagagta cgttcggaga attgtagtga gaaagggacg aagccttctc cacacggtcg
     7861 cgcagcatgc ggttgtagat gatgtggtag gagcctacaa ggccagccag gttaaaggtc
     7921 ttggatggtg cataaagtgc gatggtgcgc tcgcgtgcat ccttcgaaac ggactgcgtc
     7981 gggatatgtt tgaagcccgg cagtgtcagg tcagaccaga tctcatcgga gatgacaacg
     8041 cattcatttg ctttgtaaat ttccatcgcc ttttcaattt cctcgcgctc ccagacacgg
     8101 cctgtcgggt tgtgcggaga gcagaaaatc gcgaaatgaa tgttgtgttc ttttaacttg
     8161 cggtccatat cggcgtagtc tatacgccat acgccgttct catcctgaac aagatcggag
     8221 agaacgatat tgcgtccgca gttctcaaga acaccggtaa aaccgatgta tgtcggggaa
     8281 tgcagaagaa tggattcgcc cggggatgta aaagcggcag cagccgaagc aacacagccg
     8341 agcacgccgt tctcatagcc gatcgcctcg cgcggcagat cttccacacc gttgcgctct
     8401 ttctgccagc ggatgatatt gtcaaaatat gcatccggca gatcaaagta gccaaaatgc
     8461 ggttcgttga ctctcgcgtg aattgcttcc tgaatggtcg gaagagacgg gaagttcatg
     8521 tcggccaccc acatcgggat gcgggagaag ccctctttga cttccgcacc cggaatcggg
     8581 atctgatcga cagcgatcga atcgtggccg ctgcggttca tcagagatgt aaaatcgtat
     8641 ttcattggta aaaaccctcc tttgtgtttg ctgtgctctc gaagtaaaac caaaagcaca
     8701 tggaaaaccg gatgcagctt cctttcctcg ctgaaagaaa aacctgcagc cggctgaata
     8761 caaatattat aacacggagg ggaattttcg taaacaaaag aatgctttat tcggcaccat
     8821 ttattccgcg ccgatccagt tatcgcggtt gattgcttcc gtgtggctgg tgctttcgtt
     8881 catgattttc tgcagatccg gccggtcata gttgacaatg ccctttccga gatattcatg
     8941 gctgccgcgc cggaagacgc tgacgacatc gcctttctta aaatcgcctt cgactgccac
     9001 gatgcctttg ggaagcaggc tctttgcctg aacagcaagt gcttctgcgg caccctcatc
     9061 gacgtagaga ttgcccgcac tcggcgcata gaacgccatc cactgcagac gcgttttcat
     9121 gttgtggccg ctggcggtaa agtacgttcc ttttgcggtt cctttcaccg cacgcacaat
     9181 gttgttttcc tccgccgagg agcagatcag aaccggcaca ccggctcttg tggcaagcga
     9241 agcggcggag agcttcgttg tcatgcctcc cgttccgttg cccgatcccg caccgcctgc
     9301 cgcagcagtg agctccggcg tgatctcatt taccacgtcg atatggcgcg cattcgggtc
     9361 actctttgga ttggcagtgt agagcccgtc gatatcggtc agcagaatca gaagcgatgc
     9421 atgcagcatc gcagccacct gggcggagag cgtgtcgttg tcgccgacct tcagctcctc
     9481 aatcgcgatc gtatcgttct cattgatgat cggaatcgca ccgcgcccga gcaggacgga
     9541 gagggagccg aagatgtttt tatagcggcg gcggtcctca aagtcactgc ggttcaaaag
     9601 aatctgcgca cacggaatct ggtacgccat cagatttttc gtgtattcct ccatcagaag
     9661 gccctggccg acagcagcgg cagcctgttt atcggcaatt ttcgtcggcc gttttttaaa
     9721 gccgagacgg gaaaagcctg cggcgatcgc accggaggaa acgagaatca catggtggcc
     9781 tgccgtctgc agcgcggcaa gctgggtcgt aatgttccgt attttttcag catccatcgt
     9841 tccgtctgag agtgtcaggg agctggtgcc gactttcaca actatcgtct gtttttccat
     9901 catgaaaacc tcaaatgtaa gaaatgataa gaaacgttgt ttacgggtga caaaacatca
     9961 attacagaca ctttaacaca gcactgtgtg agagtcaaga agatgcggaa tggtcgttac
    10021 atttttcgtg aaagatctgc aaaaagatag aaatttgttg ctggaattct gaaaaagtat
    10081 ggtagaatag aaaacgagaa aaaggtgtgt gcagaaatac atttttcctg aaaaacaaaa
    10141 gaagcggaaa gaagtccggc acacgacgga gggagtcaaa gatggaggaa aaaacagaag
    10201 aaagaggcag aggctgcatg acaacgctgt gctacatcga aaaggatgac agctatctga
    10261 tgatgcaccg cacggtgaaa aagaacgatg tcaacaagga caagtggatc ggcgtcggcg
    10321 gccatgcgga acttggcgaa agcccggagg aatgcctgct gcgtgaagta aaagaggaaa
    10381 ccggctacac gctgacgagc tggcgtttcc gcggtctcgt tacctttgtg acagaggcag
    10441 aaaacagcaa aacggtggag tacatggagt atatgtgtct ctacacggca gacggattca
    10501 cgggagaacc gactgcatgt gatgagggcg aactcgcctg ggtgaaaaaa gaggacgtcc
    10561 tgcacctgaa tctgtgggag ggagacaaaa tctttttccg ccttctgaat gaagacgagc
    10621 cgtttttctc cctgaagctg cggtatgtgg gcgacacgct cgcagaagcg gtgctgaacg
    10681 gaaaacagat ggaacttttt gaggagcgct caggcgacgg cacgccgacc ggaaccatcg
    10741 tagaacgcgg cgtggcgcac agtgagggac ggtgtcacgg aacggcacat atctggattg
    10801 cacgggcgaa tgagaagagc ggctgtgagg tactgctgca gaaaaggagt gcctggaaag
    10861 attcgaatcc gggctgctac gatatttcaa gcgccggcca tctttcggcg ggcgatacat
    10921 atttggaagg ggctctgcgg gaaatcggcg aggagctcgg catccacgca gaggcagagg
    10981 agctgaggga tcttggcctg ctggaaaaag tatcacacgg tgttttttac ggaaaaccgt
    11041 tccacgatca tgaggtcagc gccgtgtatc tttacacgaa gccggtcgag gcggaaaaac
    11101 tgcatctaca ggagtcggaa gtagaagcag tccgctggat ggatctcaaa gagtgccaga
    11161 aggcggtaag agaaggaagc attcccaact gtattgatat ccgggaactg gaaatgattg
    11221 aaaaagcgtg gttcgccgga gaaaaaacaa aagatgaggc agaaaaagag gcagcagcca
    11281 ccgctgaaaa ataaaaagaa atgcatgaaa aaagtcaccg gttcgaaata gaacatggtg
    11341 acttttttgg atatcgcgta aatgaaattt gtttttttat ttcgctgctg cctttacctg
    11401 gctaaacagt ccggatttct gaagagccgg acgcagtgcc atgtagatga caacggaagc
    11461 gatggtggaa agtacggcgt tgatggaagt tgccgctacg ttccagctta aggtaacctc
    11521 tgctgccggt tttccaagaa gaaccagttt gtagaagtaa ccgatgagcg gatcgaagat
    11581 cacattgaac agaagtccgc cgatggctgc aagcagtgcc catttgaaga tctgtttctg
    11641 atcggtctgc tcgttgattt taccgatttt gtgtgcgatc agtcctgcga tgaggccgat
    11701 gcagaatttt aagatgaagg tctttggtgc gtataccaca tagaccggat cgagaagatc
    11761 tccgatggtc atgccgatcg caccgccgat tccgccgtaa acaccgccga gaatcagtgc
    11821 accgagcaca cagacagcgt ttccgaaatg gatcgaggtg gcatctccgc ctggcagcca
    11881 gattttgaac tgcaggaagg tgaagacaac gtaggaaagg gctgcaaaga cacctgtcat
    11941 tgcgattttc cagacttttt catttttcat aacaactcac tcctttggta tctggaactt
    12001 tcgttccttg attgcaccct attataggga gtggtggcat gaagaaaaag ttccaattcc
    12061 ggattttttg accagaccag ttatttccgg tacgatttca ttttttcgtg ttcttctttt
    12121 aattctctgt agagctgcag cgaattccac gcctggttct gcgttgtgac gatggctttg
    12181 agggcgatcg gcggcacttt tttccggcgc atttcggtga gaaaaagatc gagtttccgc
    12241 tcggaaaagc cggccatgac catcattggt tcatcaaatc cggcgccgtc ataggcttcg
    12301 ggggattctg caaatgcttt atttccggca agaaatccga tcgactggag atactgttcc
    12361 ggttccacaa tgcgcacgcg gataccgagc tttaagagaa ccggctttaa aagcgcagct
    12421 gttttcgttc ctttgatctg atataagagt acggtttcca tgagaaaaat cctttccacg
    12481 ggcagatttc cggatcaaat gcgcggatgc ccagaactat gatataagaa atgttttgga
    12541 aaaacaatac ttttatggta tcataggaac gtacaattca gaacacgata aattttgaga
    12601 taaaagagaa aggaaaccaa taaaatgtgg atagcagatg gatggaaaga ttatgaagtg
    12661 ctggatacgt ccggcggaga aaaactggag cggtggggag attaccttct ggtaagacca
    12721 gacccgcagg tcatctggaa cacgaaaaaa gaaaatccgg gctggaaaaa gaaaaacggc
    12781 cattatcaca gaagcagcaa gggcggcgga gaatgggagt ttttccatct gccggagcag
    12841 tgggagatca actataaaga cctgaccttc cgcctgaaac cgttcagctt caaacacacc
    12901 ggccttttcc cggagcaggc aacgaactgg gactggttcg gcgatctgat ccgccgtgcc
    12961 aaccgcccga ttaaagtgct gaacctgttt gcctacacgg gaggcgcaac gctcgcagcg
    13021 gccgcagcag gcgcaagcgt cacacatgtc gatgcatcca aaggcatggt caactgggca
    13081 aaagagaacg ctgccgtgtc cggcctttcg gacaagccga tccgctggct tgtggatgac
    13141 tgcaccaaat ttgtcgagcg cgagatccgc agaggcaacc actacgacgc gatcatcatg
    13201 gacccgccgt cctacggaag aggaccaaag ggcgagatct ggaaaatcga ggagtcgatt
    13261 catccgttca tcaggctctg cacgcagctc ttaagcgatg acccgctgtt cttccttgtg
    13321 aattcttaca cgacaggact tgcaccggct gtcctgacgt acatgatttc cctggaatta
    13381 aaacagtaca aaggccacgt agaagcacag gaaatcggcc ttccggtcaa gtcgaccggc
    13441 cttgttctgc cgtgcggtgc atcgggaaga tggcagtctg acaacgttta aaacatcttt
    13501 tggcagcgga cgtatgtccg tcatacgaaa aacatttggc tgaattgcag gaaatgaagt
    13561 ataaaactgc aaaaagtggg ataaaatgcc gaaaagtgca taaaacatgc aagttctcct
    13621 tgacagaaac tcatgatctg gtaaaatggt ctttagttga gcaagaaacc tgaaaaacgg
    13681 gtaaggaaag tacaagggag gacgtttcat gaaaccgtat atcgaatgga ccaaagaaga
    13741 attagttgcc gagaaagcca gactggagca ggaatttgca gaggtaaaag caaaaggcct
    13801 caatctgaat atttccagag gaaaacctgc agcagcccag ttagatctca cggaaggaat
    13861 gcttaccgtt ctctcgaaaa atgaagacac cttcgcagaa gatggaaccg actgccggaa
    13921 ctacggcgtg ctgaccggta ttccggaagc aagaaaactg atcggagata tctgtgaggt
    13981 tccggcggaa aacgtcatcg tttacggaaa cgccagcctt accattatgt ttgataccgt
    14041 agcacgttcg ttcctgaagg gcgttgcagg ctgcactccg tggaacaagc tggacaaagt
    14101 aaaattcctc tgtccggtac cgggctatga ccgtcacttc ggtgtaaccg agtacttcgg
    14161 cgttgagatg atcaatgttc cgatgacgcc ggaaggaccg gacatggaca tggtagaaaa
    14221 actcgttgca gaagatgacg ccatcaaggg aatctggtgt gtaccgaaat actccaaccc
    14281 gcagggctac acctactccg atgagaccgt cagaagattt gcaaacctga aaccggcagc
    14341 caaagacttc cgtattttct gggataatgc atatatcatc catcacctgt atgatgacag
    14401 acaggatcac ctgctgaaca tcatcgagga gtgcgagaaa gccggaaatc cggatatggt
    14461 ctatgagttc gtatccacct cgaaagtaag ctatccggga gccggaattg cagctctcat
    14521 ttcttctgag gcaaacctca aagaggcaca ggagtacatg aacttccaga tcatcggtca
    14581 tgataaactg aatcagctgc gccatgtaaa attcttccag aacctggacg gcgtgatgaa
    14641 tcagatgaaa aagcatgctg agatcttaag accgaaattc gaggcaattc tgcaggtaat
    14701 ggatcaggag ctgagcggcc ttggcatcgc ttcctggaca aaaccgaacg gtggatactt
    14761 catctccttc gatgcgatgg agggatgcgc aaagaagatc gttgcaaagg caaaagaggc
    14821 cggtcttacc atgacaggcg cgggcgcaac gtatccatat cacaacgatc cgcaggattc
    14881 caacatccgt attgcaccgt ccttcccgac accggaagag ctggaaatcg caggtcaggt
    14941 attcgtgctg agtgtcaaac tggtcagcgt ggataagatt ctggaaagca aataacagat
    15001 aagtaaaaat acaaaagccg gattctcccg ggggacacag tttttgtgaa ctctggtgtg
    15061 ggagttccgg cttttttcta ttagaaagtt aaaaagaaat tttctatggg acagaaagga
    15121 aaaacatgaa aaagaaagta ataggaattg ccgcaggact catactcctt gcggccgcag
    15181 gcggttacgc gggcgtgggg cattactatg catcccactt tttcccgaaa accgcgatca
    15241 acggactgaa ctgcgcaggg ctgaccgcag agcaggtcaa agaggaaatt cagaagcaca
    15301 ttgtggatta tacactcagc attaccgagc gcggtggaaa aacagagaca ttaagcggca
    15361 cggagatcgg cctcacgtat gtggacgacc acgcggtgga aaaactgctg gagtcccaga
    15421 atactcttgc atggcctgca ttctattgga aagagaaaga aaaccaggtg gcggcggact
    15481 ctgtctatga caaagaaatg gtgcaggaaa aactgcagac gatggaaggg tttcaggaag
    15541 aacagcagga agcgccgacg gatgcgtatc tgacggatga cggaacctcg tacgtcattg
    15601 tgccggaaac agagggcgag caggtggact atgagaaggc ggagcaggct gtcatcgagg
    15661 cgctggatgc cggagcggcg agcgtggatc tggaagaaaa agatgtttac cggaaaccgg
    15721 gcattacgca ggatgatgaa gcactgaatg gggaaatggc ggaactgaac cacctgacag
    15781 cggcgcggat cacgtatgca atcggtgaaa acagctatgc catagaccgg gcgaccttac
    15841 agagctggct cgtacagggg gaggatggaa cctacacgat ttcgcaggac gaagcggcag
    15901 cttttgtgcg ccatatggcg tatgagacgg acaccttcgg gctggcgcat accttcaaaa
    15961 cgagtcttgg cgcgaccatt aacctgaatg cgggcggcga ttacggctgg tgcatcgaca
    16021 aggaggagac gacgcaggca cttctgcagg cgatctagga cgaaacacag ggagatctcg
    16081 acccggttta cctctatacg gccaatgacc gctcggcaaa tgatatcgga aatacatacg
    16141 tcgaggtgtg catttcccag cagaaaatgt ggtgctacaa ggatggcgtt ctggtgacgg
    16201 aaacaccggt cacaaccggc aatcatgcga cgggctatga cactccggca ggcagtgtct
    16261 gggcggtgga tgcgaaaaaa agtgactgtg atttcaagct ctatccgagc catgtcatgt
    16321 tctggcttcc gtttaacggc gacgtcggaa tccacgatgc gagctggcgt accgagtacg
    16381 gcggggacat ctatctgacg aacggatccc acggatgtgt gaatacccca tacacagaag
    16441 cagaaaaagt gttcaatgct attgaaattg gtgacccggt tatcgtatac tatagcctgg
    16501 atgatgtaac cgggccgcag cctacacaga aaaacagcct gtaggaaaaa cgcttaagca
    16561 tcacagcaga agcacatcaa ataaaggaga ttttgataga tgaaaatagt agtattagct
    16621 ggaggaacca gcacggaacg ttcggtatcc attacatcgg gaactatggt ctgcaaggca
    16681 ctccgcgaga aaaaccatca ggcagttctg gtagacgttt tctgcggtat cgatgtgaag
    16741 gggaacctgg aagatgtatt tgcagaggag gcgtacgata tagacaaagc agcagaatat
    16801 atgcacagct ttgatgcagg attaaacgaa atgatcgaat cccgccgcag atttttcgga
    16861 ccgaacgtac tggaactctg cgaggcagcg gatgttgttt tccttgccct gcatggcgca
    16921 aacggagagg acggaaaggt acaggctacg ttcgatctgc tcggcattcc gtataccgga
    16981 acgggctatt taagcagcgc cctcgcaatg gacaaatcca tcacaaaaca gttcttccgt
    17041 gcacaccatg tgccgacccc gaacggcgtg accctgaaaa aaggccagga gagcgagcct
    17101 ttgtccggct ttggcctgga gcttccggtt gtcgtaaaac cgtgctgcgg cggttccagt
    17161 gtcggtgtct atattgcaca cacgaacgaa gaatactggc aggctatgaa ggatgctttc
    17221 tcctacgaac cggaggttgt cgttgaggag tatatcgagg gaagagagtt ctccgttggc
    17281 gtggtagacg gaaaggctta cccgatcatc gagattgcac cgatcgaagg cttctatgat
    17341 tacacgaaca aatacaaagc cggagctact gttgatacct gcccggccgt actgacggag
    17401 gagcagacga aagaaatgca gaagcacgca gaggacggcg ctgccgctct cggtatcgaa
    17461 aactactgcc gtctcgactt tatgatgcgc aaggacggaa gaatgtactg cctcgaggca
    17521 aacacccttc cgggcatgac cccgaccagc cttctgccgc aggaggcaca ggtactcggc
    17581 atttcctatc cggaactctg cgagctgctg atccagcttt ctctcagaaa aagaggtaac
    17641 aacgcatgaa aaatatgaca cttgcgcaca tcacagaggt gtgcggcgga atctacttcg
    17701 gaccggaaga aaagaaaaca gcggaagtca cggatatcgt gacggacagc cggaaggcgg
    17761 gagagggaag cctcttcgtt gcaattccgg gtgagcgtgt ggacggccat aaatttatcc
    17821 cgaacgtggc ttcccagggc gcacttgctg tcatttccga gcagaaactt gaaacaccgc
    17881 catgcccgta tatcctcgtg aaggattcca tggaggccat taaagcaatg gcagaatact
    17941 atatgcagca gctcaatatt ccggttgtcg gcattacggg aagcgttgga aagaccagca
    18001 cgaaagagac gatcgcgtcg gtgctggcgc agaaataccg tgtgctgaaa acggatgcga
    18061 acttcaacaa tgagctgggc ttgtcgctca ctgttttccg tctgcgtgag gaagatgaga
    18121 tggcggttct cgagatggga atcgacgatt tcggccagat gcaccgtctg gcgaagatcg
    18181 cacgcccgga gaccgcagtc attacgaata tcggctggtg ccatctggaa aatctgaaga
    18241 cgcgagacgg tattttacag gcgaagaccg agatctttga tcatctgcgt gagaacgggc
    18301 acattatcat caatggggac gatgataagc tgtctactgt tggaacggtt cacggcatca
    18361 agccggttca tttcggattg gatgaaaaaa acgaatatta tgccgatgag atcgagagcc
    18421 agggcttccg cggcatttcc tgccggattc ataccccgca tgggagcttt tctgcactga
    18481 ttccgattcc gggacgccat atggtttaca atgcgctggc cggaaccgca gtcggatgca
    18541 cgtacgggct gacgctggag cagatcaaac agggaatcga gacactgcag tcggtgagcg
    18601 gaagattcca catcatcgag acaggaaaat acaccgttgt cgatgactgc tacaacgcaa
    18661 acccggtttc gatgaaggca tctctcgatg ttctgaaaga ggcgaaggga agaaaagtgg
    18721 ccgtgctcgg cgatatgggc gagctcggaa ccgatgaggc agcactccac gccgaagtcg
    18781 gcacacacgc cggaacctgc ggcattgacg ccctctactg tgcgggaccg ctctgcgagc
    18841 atcttgcaaa agcggcaaaa gaggc
//
LOCUS       DAOMUF010000020        18339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_567_length_18339_cov_3.321921, whole genome shotgun sequence.
ACCESSION   DAOMUF010000020 DAOMUF010000000
VERSION     DAOMUF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 18339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18339
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_567_length_18339_cov_3.321921"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLN77_02490"
     CDS             <1..489
                     /locus_tag="PLN77_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM43184.1"
                     /translation="EIGIVQQDVYLFAGTIIDNIRYGRPDATDEEVIRAAKAANAHEF
                     IMELADGYDTDIGQRGVKLSGGQKQRLSIARVFLKNPPILIFDEATSALDNESEKVVQ
                     KSLELLAKDRTTFVIAHRLSTIRNAKRILVLTENGIAEEGTHKELLAKGGIYAHLYEM
                     QF"
     gene            605..874
                     /locus_tag="PLN77_02495"
     CDS             605..874
                     /locus_tag="PLN77_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HRM43185.1"
                     /translation="MDSSRMNRGCCGANNMQNRRAASNNMELKMEPRNFPRKMQHVDH
                     MIPAMAYVPMQRFEQIYDLSTALKNGTLFPELCKPFCGKGGGECL"
     gene            874..1152
                     /locus_tag="PLN77_02500"
     CDS             874..1152
                     /locus_tag="PLN77_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HRM43186.1"
                     /translation="MPQMPSREKLLAWIDQVSFAVVEMNLYLDTHPEDEDALAFFREK
                     VELRKEALKQYAEQYGPLTIDTANDRMSRSFEWVMQPWPWEVNRKGGC"
     gene            1156..>1222
                     /locus_tag="PLN77_02505"
     CDS             1156..>1222
                     /locus_tag="PLN77_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJC"
                     /protein_id="HRM43187.1"
                     /translation="MWNYEKRLEYPVNITTPNPKIA"
     assembly_gap    1223..1232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1233..1838
                     /locus_tag="PLN77_02510"
     CDS             1233..1838
                     /locus_tag="PLN77_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HRM43188.1"
                     /translation="MFTVTTYHLVLYSGGPDGEMGASMRYLSQRFTAPNRIVAGVLND
                     VGTEELAHLEMVSTIVHQLTCNLSLEEIQNSGFANYYVDHTTGIWPQAAGGVPFNSCE
                     FQSKGDPLTDLFEDLAAEQKARSTYDNILRLVKDPEVADPIRFLRAREVVHFQRFGEA
                     LRSVQDELNSKNFYAFNPSFDAKTFCAAPQPGAGQGNCCTR"
     gene            2004..2294
                     /locus_tag="PLN77_02515"
     CDS             2004..2294
                     /locus_tag="PLN77_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRM43189.1"
                     /translation="MEAMNYVKPELIVVAFVLYFFGVALRQAQAVKNKYIPLILGGIS
                     MVLCAVWVMATSEIETAKEGAMAVFTAVTQGILVAGLSNYVNQIIKQIHKPE"
     assembly_gap    2345..2354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2472..3521
                     /locus_tag="PLN77_02520"
     CDS             2472..3521
                     /locus_tag="PLN77_02520"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM43190.1"
                     /translation="MVVHNTDNFAAGADARAHARAQHDGNFQNISAHYYVDDGDTAYQ
                     AAPHSRGCWHVGINYGGKNLFQQYGNKNSIGVEMCVQAGYNYEKAFENTAALVREIMR
                     ETGIPLERVYRHYDICSKYCPSQIMNRGDWDRMKRMIGSGAGSTGTGTAGSGTGKTYA
                     PGIYQVQTAALNIRQAPDADSRIAGTIRDQGSYTVTEIQNTSWGRLLSGAGWVNCHTA
                     YCRYAGPAKEKSAETAKSSGKTVAEDGIWGENLTRRLQELFGTPQDGKISNQLAVNRK
                     FCDGITAAEWDSTPKGGSALVKEMQKWASAGMDGYIGPQTILAWQKKLGTPIDGTVSS
                     PSAMVKKLQKWCNQK"
     gene            3710..5557
                     /locus_tag="PLN77_02525"
     CDS             3710..5557
                     /locus_tag="PLN77_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HRM43191.1"
                     /translation="MRKKAVKNAGMIRIYLNMAWKLLQKNLLSIVIFETVYRLFSNQL
                     VSRLANAAINFSLKQLGTSYMTSENFNKIMLHPLTLLLIFGILLVFFFCMLFEIYAVM
                     AALEASWKRKRISVPVMMLAGGRGAAQFVRARPWTWFFYMAASFPYLWLHSSYSAIRS
                     MKLLQVSLTKIFNAFPAYWIPVVLTILLVAFSFVFSYTIPFRCMMTEKEKNTHLRVRQ
                     TLEKRVLRELGINVFFQVMIFLITWVLYLIFGTAVVAYAKLVKTPSTVVSTVIVYGDW
                     VKSTMSLIGGAFGLVGSITYLYLIFIRSSRKGYQKSRTTKKPSSKLVRTFCGPVTAVV
                     LTIAMLAGETVYLVYAMRATRAEATASSQYISVSAHRGGARKAPENTMSAIKYAVDSM
                     SDYAEIDVQETSDGEIVLMHDTNLKRTTGLNASIWTLTYDEISQLDAGVRFNKKFRGE
                     QIPKLEEVIAYAKGKINLNIEVKYNGHNQNIVKKVVKIIEDNDFVDQCVLTSMNYNFL
                     RQAKKINPDIKTGYTMKMSYGGLEDMDAADFFSVKYTYITESFVEHAHSLGKEVCAWT
                     LNYQGDMQRMVNCGVDNIITDDPELVRKVILGTTDRNPGFVSLLGYALK"
     gene            5588..6820
                     /locus_tag="PLN77_02530"
     CDS             5588..6820
                     /locus_tag="PLN77_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM43192.1"
                     /translation="MAEKVNMPSQKKEKKKKRKKKLPSDFYDYNLLACTILLVSFGLI
                     MLYSASAYEAASTFKGNDMYYFTHQAGLSAIVLMGIVILSKFVDYHSVWFQRIAALVY
                     WGSLLLMAAVRFTPLGYEAYGARRWLRIGGVSLQPAEIGKLGLILYLPYIILKMGKNM
                     RTIRAKAIVLGLGVLQALAAWILTDNLSTAIILGLIACVILFLADPEVKFYARVIPGA
                     AVIGVFAIIILKNNLQIFERIGGDFRSNRIYEWLSGGSYQILQGLYAIGSGGFLGKGL
                     GNSTQKITTIPEAQNDMIFSIICEELGIFGALLVLLLFGYLLYRLFVVAQNAPDAYGM
                     LMVSGVFAHISIQVILNLCVVLKLMPATGITLPFISYGGTSVLFILTEIGIALCVSRF
                     IVFQDGKSGEGEAQAEEQ"
     gene            6916..7149
                     /gene="acpP"
                     /locus_tag="PLN77_02535"
     CDS             6916..7149
                     /gene="acpP"
                     /locus_tag="PLN77_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRM43193.1"
                     /translation="MLEKIKEILVEQLNCDADSINEDTSFKDDLGADSLDLYEMVMAL
                     EDEYGIEIDTDELTDLSTVGDFMEYLKQHGVEV"
     gene            7292..8743
                     /gene="gltX"
                     /locus_tag="PLN77_02540"
     CDS             7292..8743
                     /gene="gltX"
                     /locus_tag="PLN77_02540"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRM43194.1"
                     /translation="MSNVKTRFAPSPTGRMHVGNLRTALYAYLIAKHDDGTFMLRIED
                     TDQERFQEGALDIIYRTLKETGLVHDEGPDKDGGCGPYVQSERNAAGLYLKYAKQLVE
                     QGDAYYCFCDAERLSQCKRNVGGKEISIYDKHCLGLSKEEVEANLAAGKPYVIRFNMP
                     TEGTTTFHDEIYGDITVNNEELEDLILIKSDGYPTYNFANVIDDHLMGVTHVVRGNEY
                     LSSSPKYNRIYEAFGWEVPVYVHCPLITNEEHQKLSKRCGHSSYEDLIDQGFLKDAIV
                     NFVALLGWSPEGNREIYSLEELVKIFDYHHISKSPAVFDMTKLRWMNGEYMKAMDDEK
                     FYEMALPYIQKTIHRPLDFKKIAAMVKTRIEVFPDIADLIDFFEEVPEYDASMYTHKK
                     MKTNEETSLALLQEVKPLLAAQEDFSNDALFEMLSAFGKEKGYKTGYIMWPIRTALSG
                     KQMTPAGATEILEVLGKEESLARIDKAIEKLSK"
     gene            8740..10560
                     /locus_tag="PLN77_02545"
     CDS             8740..10560
                     /locus_tag="PLN77_02545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRM43195.1"
                     /translation="MIYNNSQKKAVMHTTGPMMVLAGPGSGKTAVITGRTCQLVTSGI
                     SASRILVVTFTRAAAKEMKERYLKAMGKTSTQVTFGTFHGVFYAILRHTYRMSGNNIL
                     SEEEKKRLMRELVNHYARDLEEEDLEENLAREISTVKNNQIPLEHYYSACMPQEKFRE
                     IYEAYEKWRKENKKLDFDDLMVQCKRLFLERPEVLRAWQQKFQYILIDEFQDISPAQY
                     EIVRMLALPENNLFIVGDDDQSIYQFRGAKPEIMLNFPKDYPGAAQVVLDKNYRSTEF
                     IVKRSQCLIHHNKKRYEKAISTDNEKGAPVAIRGYKNPREEMRAVAEELREAEKNGCP
                     YEEMALLFRTNLGCRTAVEELMEAQIPFQMRDALPDLYDHWIAKDLLTYLNLAMGSRK
                     RGEFLKIANRPNRYLSRDAFEEATVSFDALLEYYEEKDWMCQRIRKLEQDITTLTHLS
                     PFGAVNYIRYAIGYEQYVKEYAAYRHLKEDDLISVLDELQEAAKSQKSIEGWFAHIEE
                     YQQKMKEKQKQRAESAEGVTVSTLHSVKGLEYDKVYLLDVNEGVMPYQKAVLAEAIEE
                     ERRMFYVGMTRARKELTLCYVEERFEKKVEPSRFLDEVIQ"
     gene            10711..11703
                     /locus_tag="PLN77_02550"
     CDS             10711..11703
                     /locus_tag="PLN77_02550"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRM43196.1"
                     /translation="MKILFYDTQSYDRESFDRTKEQFPEIEVEYLKTGLAARTASLAK
                     GYDAVCAFVNSDVGTKTVEALHEAGVKLILMRCAGFNNVDLKTAAKYGIDVRRVPGYS
                     PEAVAEHAMTLAMTVNRHMHKAYTKVRENDFSLGGLMGFNFYQKTAGIVGTGKIGAAM
                     ARICRGFGMKVIAYDVYQNESLKDFVTYVTLDELLAQSDLISLHCPLMDSTYHMINRE
                     SIKKMKDGVILVNTSRGGLVKTDDLIEGIRARKFFGVGLDVYEEETPNVFEDRSDEIL
                     EHSTTARLLSFPNVMITSHQGFFTEEALRAIAETTLQNALDFEAGKETPNIVRA"
     gene            11867..12226
                     /locus_tag="PLN77_02555"
     CDS             11867..12226
                     /locus_tag="PLN77_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRM43197.1"
                     /translation="MDCKEAEKLIQPYVQGNMPEKEMEPFISHIRKCHTCHEELETYF
                     IVNRAMAYFEDDAPDSYNLTGLLERDLEKKEEEARHRRYKDTFFRVLMLILVLFLVLL
                     ALHYFEVIELPWLKGLL"
     gene            12223..14898
                     /gene="polA"
                     /locus_tag="PLN77_02560"
     CDS             12223..14898
                     /gene="polA"
                     /locus_tag="PLN77_02560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRM43198.1"
                     /translation="MSDKIVLLDGHSILNRAFYGVPDLSNAEGLHTNAVYGFLNILFK
                     ILDEEQPQYLAVAFDLHAPTFRHKMYDQYKGTRKPMPQELREQVPVIQEVLAAMDIEI
                     VTKEGYEADDILGTLGRKCEAEGMEVTIVSGDRDLLQLATDHILIRIPKTVKRVTTIE
                     NYHTAEVLEKYSLLPKQIIDLKALMGDTADNIPGLPGVGEKTATKILLQYETLENAHA
                     HFEEIKPNKAKEAMRDHYDLAELSKKLATIDTDAPVELDREKAALSNFYTPKAYEMFK
                     RLEFKNLLGRFEETNAEPEDAVFLRTVTDFSEAEELFETIAKEEKAGAALLTEETPKD
                     GPMADRSRSLVGMAVAYGSGEPDVVYFPAEGFLTGDYLKEKLTELQKQIPVFCVMDGK
                     EFLKDMPDADEAHLFDAGIAAYLLNPLKSQYSYDDIVKEYVHRYVPAVEEIFGGSKIP
                     AAGKMTPEQQESYAGHQAYAVFAAQENMEKLLKEQGMWDLYRNVEIPLVFTLRQMEAD
                     GIAAEKEALSVYGAELAERIGELEAQIYEEAGEEFNINSPKQLGVILFEKLQLPGGKK
                     TKTGYSTAADVLEKLAPDHKLVADILEYRQLAKLKSTYADGLQAVIGKDGRIHSTFNQ
                     TITATGRISSTDPNLQNIPVRMELGRLIRKAFVPKPGCVFLDADYSQIELRVLAHMSG
                     DENLIEAYREAEDIHRMTASKVFHVPLEEVTPLQRRNAKAVNFGIVYGISSFGLGQDL
                     GISRKEAEAYIQQYFETYPGIHEFLNRCVEDAKEKGYSVTMFGRRRPMPELKSSNFMQ
                     RSFGERVAMNAPIQGTAADIIKIAMIRVAKRLKEEQLKTRLLLQVHDELLLEVPEDEL
                     EKAEQILEEEMTGAAHLAVKLEIDMHTGNNWYEAK"
     gene            14933..15532
                     /gene="coaE"
                     /locus_tag="PLN77_02565"
     CDS             14933..15532
                     /gene="coaE"
                     /locus_tag="PLN77_02565"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945091.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRM43199.1"
                     /translation="MKVFGITGGVGAGKSEVLTYLGQNYDATVIQADEAGYLVMLPGG
                     ECYGEIVKLFGRQITTETGELDRKRIAEIVFQDEEKLKALNAIVHPAVKRYIKKAIAA
                     AEKAGTEYVFVEAALLIEEKYDEICDELWYIYADEEVRKERLIEGRGYSEKKVREIMA
                     NQLSEDEFSSHCDFEIDNSGDFEETKKQLDHRMKKYETL"
     gene            15519..16316
                     /locus_tag="PLN77_02570"
     CDS             15519..16316
                     /locus_tag="PLN77_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM43200.1"
                     /translation="MKLCSIASGSSGNCIFAGSETTSLLIDAGISGKRVEAGLAVLDR
                     SAKELDGILVTHEHSDHIQGLGVLARRYGIPIYATDGTIQAMQQTKSLGKFPEGIFHT
                     IHADDTFTLGDIAVHPFTISHDAAEPVGYRMESGGKSIAIATDLGVYSDYTVQNLTGL
                     DAVLLEANHDENMLAVGPYPYPLKQRIAGAKGHLSNLAAGHLLCEILHDNLQEVLLGH
                     LSKENNYEALAFETVACEVTTGDNPYHSGDFHLAVAKRSEVSPLIEV"
     gene            16416..16688
                     /locus_tag="PLN77_02575"
     CDS             16416..16688
                     /locus_tag="PLN77_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRM43201.1"
                     /translation="MKKTIITVVGKDTVGIIAKVCNYLAENQVNIEDISQTIVQGYFN
                     MMMVTDVSKSEKSNAELAKDLEELGSSIGVVIRCQHEDIFNMMHRI"
     gene            16708..18072
                     /locus_tag="PLN77_02580"
     CDS             16708..18072
                     /locus_tag="PLN77_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HRM43202.1"
                     /translation="MINLFEVKETNEMIEKENLDVRTITLGISLMDCIDSNLDKLNRK
                     IYDKITTTARNLVSVGEEIEGEFGIPIVNKRISVTPIALVGAAACRTPEDFVTIAQTL
                     DRAAKEVGVNFLGGYSALVSKGMTTADELLIRSIPQALAVTDFVCSSINLGSTKTGIN
                     MDAVKLMGEIIKKTAEASKENNCLGCAKLVVFCNAPDDNPFMAGAFHGVTEADAIINV
                     GVSGPGVVKHALEKVRGENFEVLCETIKKTAFKVTRVGQLVAQEASKRLNIPFGIIDL
                     SLAPTPAIGDSVADILEEIGLEHAGAPGTTAALALLNDQVKKGGVMASSYVGGLSGAF
                     IPVSEDQGMINAVNDGALTIEKLEAMTCVCSVGLDMIAIPGDTKASTISGIIADELAI
                     GMVNQKTTAVRLIPVIGKGVGETVEFGGLLGYAPIMPVNNFSCEAFVNRTGRIPAPIH
                     SFKN"
BASE COUNT         5137 a         4161 c         4987 g         4034 t
ORIGIN      
        1 gaaattggta ttgtgcagca ggatgtgtac ctgtttgcgg gaacgatcat cgacaacatc
       61 cgctatggca gaccggatgc gaccgacgag gaggtcatcc gcgcagcgaa agcggccaat
      121 gcacatgaat ttatcatgga gctggcggac ggctacgaca ccgatatcgg ccagcgcggc
      181 gtaaaacttt ccggtggcca gaaacaacgg ctttccatcg cccgcgtatt cctcaaaaac
      241 ccgccgatcc tgattttcga cgaggcaacc tcagcgctcg acaacgaaag cgaaaaagtc
      301 gtccagaaat ctttggaact tctggcgaaa gacaggacaa ccttcgtgat tgcgcaccgg
      361 ctgtccacaa tccggaatgc aaagcggatt ctggtgctga cggagaatgg aattgcggag
      421 gaaggcacac ataaggaact gctcgcgaaa ggcgggattt atgcgcattt gtatgagatg
      481 cagttttgat gaaaatttca ttagtatgac agaaaggcag cggaagagaa accgctgcct
      541 tttttgacac tttcttattt tcttcatact ctataaagaa cagaaaatga aagaggaaca
      601 tggtatggat tcatcccgta tgaatcgcgg ctgctgcggt gcgaacaata tgcagaaccg
      661 ccgcgccgct tcaaacaata tggaactgaa gatggaacca aggaatttcc cgcggaaaat
      721 gcagcatgta gatcatatga tacctgcaat ggcgtacgtt ccgatgcagc ggtttgaaca
      781 gatctatgat ctttccactg cattaaaaaa tggaacctta ttcccggaac tctgtaaacc
      841 attctgtgga aagggaggag gagagtgcct atgatgccgc agatgccatc ccgcgaaaag
      901 ctgctcgcct ggattgatca ggtcagcttt gctgttgttg aaatgaacct ttacctcgat
      961 acccacccgg aggatgaaga tgcacttgcc tttttccggg aaaaagtgga gcttcgaaaa
     1021 gaagcattga aacagtacgc cgaacagtac ggcccgctca cgatcgatac cgccaacgac
     1081 cgcatgagcc gttctttcga atgggttatg cagccatggc cgtgggaagt caaccggaaa
     1141 ggagggtgct gacggatgtg gaattatgaa aaacgcctcg agtatcctgt aaacatcacg
     1201 acaccaaacc ctaaaatcgc ccnnnnnnnn nnatttttac agttacaaca tatcatctgg
     1261 tattgtattc cggtggaccg gatggagaga tgggcgcatc catgcggtat ctctctcagc
     1321 gtttcacagc accgaaccga attgtagcgg gggtgttgaa tgatgttggg acagaagaac
     1381 tcgcacacct tgagatggtt tcaacgatcg ttcatcagct cacctgcaat ctttctctgg
     1441 aagaaattca gaactctggt tttgccaatt actatgtaga ccacacaact ggcatctggc
     1501 cgcaggcggc tggcggagtt ccgtttaatt cttgtgagtt ccagtcgaaa ggtgatcctt
     1561 tgacagattt gttcgaagac ctcgctgcgg agcagaaagc ccggtcaacg tatgataaca
     1621 tccttcgttt ggtaaaagat cctgaagttg cagatccgat tcgtttcctg cgggcgcgtg
     1681 aagttgtgca tttccagcgt tttggtgaag cactgcgttc tgtacaggat gaattgaatt
     1741 cgaagaactt ttatgcgttt aatccgtcgt ttgatgcaaa gactttctgt gcggcaccgc
     1801 agccaggggc aggtcagggg aattgctgca caagatagta aattaaaaat caaagaacag
     1861 cacaccactg gaaaatccca tctgaaaaac aggtgggatt ttttgggttt acagtaagaa
     1921 aatcttatcc agtcgtaaat cattatccca tcataatata gaaaaaagac tttcaaaaac
     1981 agaacagtca gaaaggatgt tccatggaag caatgaatta tgtaaagccc gaactcatcg
     2041 tcgttgcgtt cgtgctgtat tttttcggtg ttgctttgcg gcaggcgcag gcggtgaaga
     2101 ataagtacat tccgctgatc ttaggcggca tcagcatggt gctgtgtgcg gtttgggtga
     2161 tggcgacgag tgagattgag acggcgaagg agggggcgat ggcggttttt acggcggtca
     2221 cgcaggggat tctggtggcg gggctgagca attatgtgaa tcagattatc aagcagatcc
     2281 ataagccgga gtgaacggcg cttcaaaaaa tctgtgttat gccggagcag tctgaaccgt
     2341 cagannnnnn nnnnattcaa tccactcttc gcttatcctg aaaatgtcgt tgtcagtacg
     2401 gcacatcctt tcgcattgat cggtcgtatc ttggcaatca gaatacgtat gcggaaaaca
     2461 atcccaaatg tatcgtagtc cacaacaccg acaacttcgc agcaggtgcc gatgcgcggg
     2521 cacatgcgag agcacagcat gacgggaatt tccagaacat ttccgcacac tattacgtcg
     2581 atgacggtga cacagcctac caggcggcac cgcacagccg ggggtgctgg catgtcggca
     2641 ttaattacgg cggaaaaaat ctgtttcagc agtacggcaa caagaacagc atcggtgtgg
     2701 agatgtgtgt gcaggccggg tataattatg aaaaagcatt tgagaatact gcggcactgg
     2761 tgcgggagat catgcgggag acggggattc cgctggaaag agtctatcgt cattatgaca
     2821 tctgcagcaa atactgtccg agccagatca tgaaccgcgg tgactgggac cgcatgaagc
     2881 ggatgatcgg aagcggtgca gggagcacag gaacgggaac agcaggcagc ggaacaggaa
     2941 aaacgtatgc gcccgggatc tatcaggtgc agacagcggc cctcaacatc cgtcaggcac
     3001 cggatgcgga cagcaggatt gccggaacga tccgggatca gggaagctac acggtgacgg
     3061 aaatccagaa cacaagctgg ggacggcttc tctcaggggc aggctgggtc aactgccata
     3121 cagcgtattg ccgttatgcc ggtcccgcaa aagaaaaatc ggcagagaca gcaaagtcat
     3181 ccggaaagac agtcgcagag gacggaatct ggggcgaaaa tctgacgcgc cgtctgcagg
     3241 aacttttcgg cacaccgcag gatggaaaaa tcagcaatca gctggccgtc aaccggaaat
     3301 tctgtgatgg catcacagcc gccgagtggg acagtacgcc aaaaggcgga tcggcccttg
     3361 taaaagaaat gcagaaatgg gcatcggccg gcatggacgg ctatatcggt ccgcagacaa
     3421 tcctcgcctg gcagaaaaaa ctcggcacgc caatcgacgg cacagtaagc agcccatccg
     3481 ccatggtaaa aaaactgcag aagtggtgca accagaaata gaaaaaagaa tactcaaaga
     3541 cccggttttg tgttatactg gaagatagtt taaaatgaac agaaagaaaa agagcttcaa
     3601 aaaagactcg aagtgtcaaa tgtaaaatac aaaagagaaa aaataaaggt aatttttgca
     3661 tacagattca tcggggggaa ttgcggctga caggagagaa caacagcaga tgagaaaaaa
     3721 ggcagtaaaa aatgcaggaa tgatacgaat ttatctgaat atggcatgga aacttttgca
     3781 gaaaaacctg cttagcatcg tgatatttga gacggtctac cgcctgtttt ccaaccagct
     3841 ggtttcaaga ctcgccaatg cggcgatcaa cttttcctta aaacagctgg gaacgagtta
     3901 tatgacatcg gaaaacttca acaagatcat gctgcatccg ctgacccttc tgctgatttt
     3961 tggaattctg cttgttttct ttttctgtat gctgtttgaa atctatgctg tcatggcggc
     4021 tctggaggcc tcctggaaac gaaaacggat ttccgttcct gtcatgatgc ttgccggcgg
     4081 aagaggagcc gcacagtttg tgcgcgcccg cccgtggacc tggtttttct acatggcagc
     4141 gagcttcccg tacctttggc tgcacagcag ctacagcgcc atccgcagta tgaaactgct
     4201 tcaggtttcc ctgacgaaga ttttcaatgc cttcccggca tactggatcc cggtggtgct
     4261 gacgattctg cttgtcgcgt tctcgtttgt gttttcctac accatcccgt tccggtgcat
     4321 gatgacggaa aaagagaaaa atacccatct gcgggtgcgc cagacactgg aaaaacgcgt
     4381 gctgcgtgag cttggcatca acgtcttttt ccaggtgatg atttttctga ttacgtgggt
     4441 tttgtatctg attttcggaa cggctgtcgt tgcctatgca aagctggtga aaacgccgtc
     4501 taccgttgtc agtaccgtca tcgtctacgg tgactgggtc aaatcgacga tgagcctgat
     4561 cggcggagcg ttcggccttg tcggaagcat tacgtatctg tatctgattt ttatccgttc
     4621 ctcccgaaag ggctaccaga agagccggac aacgaaaaag ccgagctcga agctggtacg
     4681 caccttctgt ggtccggtaa cggcggtggt tctcacgatc gccatgctgg cgggagaaac
     4741 ggtttatctt gtatatgcca tgcgcgcaac acgtgcagag gcaacggctt cctcccagta
     4801 tatttcggtg tcagcgcacc ggggcggagc cagaaaagca ccggaaaata cgatgagcgc
     4861 gatcaaatat gcagtggaca gcatgtcgga ttacgcggag atcgatgtac aggagacgag
     4921 tgatggtgag attgtcctta tgcacgacac aaatctgaag cggacaaccg gtctgaatgc
     4981 cagcatctgg accctgacct acgatgaaat cagccagctc gatgccggtg tacgattcaa
     5041 caaaaaattc cgcggcgagc agattccgaa gctggaagaa gtcatcgcgt acgcgaaagg
     5101 aaaaatcaac ctgaatatcg aagtaaaata caacggccac aaccagaata ttgtcaaaaa
     5161 agtggtgaaa atcatcgagg acaatgactt tgtggatcag tgtgtgctga cttccatgaa
     5221 ctacaatttc ctcaggcagg caaagaaaat caatccggat atcaagaccg ggtatacgat
     5281 gaaaatgtcg tacggaggcc tggaggatat ggacgcggcg gacttctttt ctgtaaaata
     5341 tacgtacatc acagaaagct ttgtagagca tgcgcacagt cttggtaaag aagtctgtgc
     5401 ctggacgctg aattatcagg gagatatgca gcggatggtc aactgcggcg tggacaacat
     5461 tatcacggac gatccggagc tggtgcgtaa ggttatcctc ggaacgaccg accgaaatcc
     5521 gggctttgtc agcctgcttg gctatgcact gaaataaatg gagtaaaaca atggaggtgg
     5581 aaacttcatg gcagagaaag tcaacatgcc atcccagaag aaagaaaaga agaaaaagag
     5641 gaagaaaaaa cttccttcgg atttttatga ttataacctg ctggcctgca ctattttgct
     5701 ggtcagcttc ggactgatta tgctgtacag cgcgagcgcc tacgaggcgg ccagtacatt
     5761 caaaggaaat gatatgtatt atttcacgca tcaggcggga ctttctgcta ttgttctgat
     5821 ggggattgtg atattgtcga agtttgtaga ttatcatagt gtatggttcc agcggattgc
     5881 agcactggta tactggggaa gtctccttct gatggcggcg gtaaggttta cgccgcttgg
     5941 ttatgaagcc tatggggcga gaagatggct ccggatcggc ggtgtcagtc ttcagcctgc
     6001 ggagattgga aagctggggc tgattctgta tttgccatac attatcctga agatgggaaa
     6061 aaatatgcgt accatccgcg caaaagcgat tgtcctcgga cttggcgtgc tgcaggcgct
     6121 ggctgcgtgg attctgacgg acaacctgag taccgccatt attctgggac tcatcgcctg
     6181 cgtcattttg tttctggcgg acccggaggt aaaattttat gcgcgtgtga ttccgggcgc
     6241 ggcggtaatt ggcgtttttg cgattatcat tttaaaaaat aatctgcaga tctttgaaag
     6301 aataggcggt gacttccggt cgaaccgtat ctatgaatgg ctttccggag gcagctatca
     6361 gattctgcag ggactttatg cgattggctc cggtggattt ctcggaaaag gacttggcaa
     6421 cagtacgcag aagattacaa caattccgga agcacagaac gatatgatct tctcgatcat
     6481 ctgcgaggag cttggaatat ttggcgcgct tcttgtattg ctgctgtttg gctacttgct
     6541 gtaccgtctg tttgtggttg cacagaatgc gccggacgcg tatggaatgc tgatggtgag
     6601 cggcgttttt gcacatattt ccattcaggt tattctgaac ctgtgcgtcg ttctgaaact
     6661 gatgccggca acgggaatta cccttccatt tatcagttac ggaggaacat cggtgctctt
     6721 tatcctgacg gaaatcggca ttgccctgtg cgtctcccgc tttattgtgt tccaggatgg
     6781 aaagagcgga gaaggagagg cacaggcgga agaacagtaa aagtagaaaa atctattgac
     6841 aaatgccgga ataacagata cacttttttc gaaagaaata ctggctgccg ggtagaggca
     6901 gaaaggagca gaacaatgtt agaaaagata aaagaaattt tagtagaaca gctcaactgt
     6961 gatgcagaca gcatcaacga agatacctct tttaaagatg acctgggtgc agattccctg
     7021 gatctctacg agatggttat ggcattggaa gacgagtacg gaattgaaat cgacaccgac
     7081 gaactgacag acctttctac cgtcggcgac tttatggaat atttgaaaca gcacggcgta
     7141 gaggtctgaa aaagagcatt gccgctggca aaagtggaag gatggtgcag ctcggaagaa
     7201 acgccgggct gttttccatt ctaaataaat ggtggaaggg aacagacaaa aggattgttc
     7261 cagaaaaacc agaaatcagg agaaaagaaa aatgagcaat gtaaaaacaa gatttgcacc
     7321 gagcccgaca ggccggatgc atgtaggaaa cctgcgtact gccctgtatg cgtatctgat
     7381 cgcaaaacac gatgacggaa cctttatgct ccgtatcgag gataccgacc aggagcgttt
     7441 ccaggaaggc gcacttgata tcatctaccg taccttaaaa gagaccggcc tggtacacga
     7501 cgagggaccg gacaaagacg gcggctgcgg cccgtacgta cagagcgagc gtaatgcagc
     7561 aggtctgtac ctgaaatacg caaaacagct ggtagagcag ggtgacgctt attactgctt
     7621 ctgtgacgca gagcgcctga gccagtgcaa gcgtaacgta ggcggaaaag aaatctcgat
     7681 ctacgacaaa cactgccttg gtctttccaa agaggaagta gaggccaatc tggcagccgg
     7741 caaaccgtac gtcatccgtt tcaatatgcc gaccgaagga accacaacct tccacgatga
     7801 gatctacgga gacatcaccg taaacaacga ggagctggaa gacctgatcc tgatcaaatc
     7861 cgacggatat ccgacctaca acttcgcaaa cgtcatcgac gaccatctga tgggtgtaac
     7921 ccacgttgtc cgcggtaatg agtatctgtc ctcatccccg aaatacaacc gtatttacga
     7981 ggcattcggc tgggaagttc cggtttacgt acactgtccg ctgatcacca acgaggagca
     8041 tcagaagctg tccaaacgct gcggccattc ttcttatgaa gacctgatcg atcagggctt
     8101 cttaaaagat gccattgtca acttcgtagc ccttcttgga tggagcccgg aaggaaaccg
     8161 cgagatctat tctctcgagg agctggtaaa aatcttcgat taccaccaca tcagcaaatc
     8221 tccggccgtt ttcgatatga caaaactccg ctggatgaac ggcgagtaca tgaaggcaat
     8281 ggacgacgag aagttctatg agatggcgct tccgtatatc cagaagacaa tccaccgtcc
     8341 gctcgatttt aagaaaattg cagccatggt aaaaacaaga atcgaagtat tcccggatat
     8401 tgcagacctc atcgacttct tcgaggaagt tccggagtac gacgcttcga tgtacactca
     8461 caaaaagatg aagacaaacg aggagacctc ccttgcactt cttcaggaag taaaaccgct
     8521 gctggcagcg caggaagatt tcagcaatga tgctctcttt gagatgctca gcgcattcgg
     8581 aaaagaaaaa ggatacaaga ccggttatat tatgtggccg atccgtaccg ctctttccgg
     8641 aaaacagatg acaccggccg gcgcaacgga aatccttgag gttctgggaa aagaggagtc
     8701 tcttgcccgc atcgacaaag cgattgagaa attatccaaa tgatatataa taattcacag
     8761 aaaaaagcgg tgatgcatac caccggcccc atgatggtcc ttgccggacc ggggtcgggg
     8821 aaaaccgcag tgattacggg gcggacctgc cagcttgtaa cgagcggcat ttccgcctct
     8881 cgtattcttg tcgtaacatt tacgcgtgcg gcggcaaaag agatgaagga gcggtatttg
     8941 aaggcgatgg gaaagacgag tacccaggtc acctttggaa cgttccacgg ggtattctac
     9001 gcgattctgc gccatacgta ccgcatgtcc ggcaacaata ttttaagcga ggaagagaaa
     9061 aagcggctga tgcgcgagct ggtcaaccac tatgccagag acctggagga agaggatctc
     9121 gaggaaaatc tggcgcgcga aatcagcact gtgaagaaca atcagatccc gctggaacat
     9181 tactattccg cctgtatgcc gcaggaaaag ttccgcgaaa tctatgaggc gtatgagaaa
     9241 tggcggaagg agaacaaaaa actcgatttc gacgatctga tggtgcagtg caaacgtctc
     9301 tttctggagc ggccggaggt cctccgcgcc tggcagcaga aatttcagta tatcctgatt
     9361 gacgagtttc aggacatcag ccccgcgcag tatgagattg tgcggatgct ggcgctgccg
     9421 gaaaataacc tctttattgt cggggatgac gatcagtcga tttatcagtt ccgcggggcc
     9481 aagccggaga tcatgctgaa ttttccgaaa gattacccgg gcgcggcgca ggtagtgctc
     9541 gataaaaatt accgtagtac ggaatttatc gtgaagcggt cccaatgcct gatccaccac
     9601 aacaaaaagc ggtatgaaaa ggcgatttcc accgacaacg agaaaggcgc tccggttgca
     9661 atccggggat ataagaaccc gcgcgaggag atgcgtgccg ttgccgagga gctgcgggag
     9721 gcggagaaaa acggctgtcc gtatgaagag atggcgcttt tgttccgcac gaatcttggc
     9781 tgccggacag cagtagagga actgatggaa gcacagatcc cgttccagat gcgggatgcc
     9841 ctgccggatc tctatgacca ctggattgcg aaagatctgc tgacgtatct gaatcttgcg
     9901 atgggaagcc ggaagcgcgg cgagttttta aagattgcca accgcccgaa ccgttatctg
     9961 tcgcgggatg cctttgagga ggcgactgtt tcgttcgatg cacttctgga atattacgaa
    10021 gaaaaagact ggatgtgcca gcggatccgc aaactggagc aggatatcac gacactgacg
    10081 catttatcgc cgttcggcgc ggtcaattac atccgctatg cgatcggcta tgaacagtat
    10141 gtgaaggaat atgctgcata ccgccatctg aaagaggatg atctgatttc cgttttggac
    10201 gagctgcagg aggcggcaaa gagccagaaa agcatcgagg gctggtttgc acatattgag
    10261 gaataccagc agaagatgaa ggaaaaacag aagcagcgcg cagagagtgc ggaaggagtt
    10321 acggtttcca ccctgcacag cgtcaaggga ctcgagtacg acaaggtcta tcttcttgac
    10381 gtcaacgagg gcgtaatgcc gtatcagaaa gcagtccttg cggaggcgat cgaggaggag
    10441 cggcggatgt tctacgtcgg aatgacgcgc gcccggaaag agctgacgct ctgttatgtg
    10501 gaggaacggt ttgaaaagaa agtcgagccg tcccggttcc tggacgaggt gatccagtaa
    10561 atttgtactg ttgtgaaaga tgttgaaaat aaatttacta attgataaaa ttttaacaaa
    10621 taatacattg acaaagtttt aacaatgaag tatagtaatg actgtaagcg ggaacaataa
    10681 gatgaccgca aagcgaggag gaaaaacatt atgaaaattt tattttacga tacccagagc
    10741 tacgacagag aatctttcga ccgaaccaaa gagcagtttc cagaaattga agtggaatat
    10801 ttaaaaaccg gccttgctgc aagaaccgct tccctggcaa agggctacga tgccgtgtgc
    10861 gccttcgtaa actccgatgt cggaacaaaa accgtagaag cactgcatga ggcaggcgtc
    10921 aagctgatcc tgatgcgctg cgcaggcttc aacaacgtag atttaaagac agctgcaaaa
    10981 tacggcattg atgtacgccg tgtaccgggt tactccccgg aggctgtagc tgagcatgcg
    11041 atgacactgg ccatgacggt caaccgccac atgcacaaag catatacgaa agtaagagaa
    11101 aacgacttca gcctgggcgg actgatggga tttaacttct atcagaagac cgccggaatc
    11161 gtcggaaccg gaaaaatcgg tgcggctatg gcaagaatct gccgcggttt cggcatgaaa
    11221 gtcattgctt acgatgtgta ccagaatgaa tccttaaaag attttgttac gtacgtaact
    11281 cttgatgaat tgctggcaca gagtgatctg atctccctgc actgcccgct gatggacagc
    11341 acataccaca tgatcaaccg cgagagcatc aaaaagatga aagacggcgt gatcctggtc
    11401 aatacgtccc gcggaggtct ggtaaagaca gacgacctga tcgagggaat ccgtgcaaga
    11461 aaattcttcg gcgtcggcct tgacgtatac gaggaggaaa ccccgaacgt attcgaggac
    11521 cgttccgatg agattctgga gcactccacc acagcacgtc tcctgtcctt cccgaacgtt
    11581 atgatcactt cacatcaggg attcttcacc gaggaagcac tgcgtgcgat tgcagagacc
    11641 acactgcaga atgcgctgga tttcgaggca ggaaaagaga ccccgaacat tgtccgtgca
    11701 taatggaaat ccactagaaa acagttatct tataagagaa caagagacaa cccattttcg
    11761 gcgggttgtc tcttttaaat tatgagaaaa tagtgtatga tggtagatag cagaaaaaac
    11821 ggccagagaa aaacactggc cggatacaaa aggagagcag gattttatgg actgtaaaga
    11881 agcagaaaaa ttaatacagc catacgtgca gggaaatatg ccggagaaag agatggaacc
    11941 gtttatttcc catatccgga aatgccatac atgccacgag gaactggaga catattttat
    12001 tgtgaaccgt gcgatggcat atttcgagga tgacgcgccc gattcctaca atctgacagg
    12061 acttctggaa cgcgatctgg agaaaaaaga ggaagaagcg agacaccgcc ggtacaaaga
    12121 tacgtttttc cgggtgctga tgctgattct cgttctgttt ctggtgctcc tggctctgca
    12181 ctattttgaa gtgatagaat taccctggct gaaaggatta ttatgagtga taaaatcgta
    12241 ttactggacg gacacagtat tctgaaccgg gctttttacg gcgtaccgga tctgtccaat
    12301 gcagaaggac tgcacacgaa cgccgtatat ggattcctga atattctgtt taaaattctc
    12361 gatgaggagc agccgcagta tcttgccgtg gcgtttgacc tccatgcacc gacattccgc
    12421 cacaaaatgt atgaccagta caagggaacg agaaagccga tgccgcagga attgcgcgaa
    12481 caggtcccgg tgatccagga agtgctcgca gcaatggaca ttgagattgt cacaaaagag
    12541 ggctatgagg cggatgatat cttaggaacg ctcggccgca aatgcgaggc ggaagggatg
    12601 gaagttacga tcgtatccgg agaccgcgac ctgctgcagc ttgcaacgga ccacattctg
    12661 atccggattc caaagaccgt aaagcgtgtg acgacgattg aaaattacca cacggcagag
    12721 gtgctggaaa aatacagtct tctcccgaaa cagatcatcg atctgaaagc gctgatgggc
    12781 gataccgcag acaatatccc gggtctgccg ggtgtcggcg agaaaaccgc gacgaaaatc
    12841 ctgctgcagt acgagacact ggaaaatgcg catgcacatt tcgaggagat caaaccgaac
    12901 aaggcaaaag aggcgatgcg tgatcattat gatctggcgg aattaagcaa aaaacttgca
    12961 acgattgata ccgatgcgcc ggtggagctc gaccgtgaaa aagcggcact ctcgaatttt
    13021 tatacaccaa aagcgtatga gatgttcaaa cgtctggagt ttaagaacct gctgggccgc
    13081 ttcgaggaga cgaatgcaga accagaggat gccgtctttc tgcgcaccgt aacggatttt
    13141 tccgaggcgg aagaactctt tgaaaccatc gcgaaggagg agaaggcagg agccgctctt
    13201 ctgacagaag aaaccccgaa ggacggaccg atggcagatc ggagccgttc acttgtcggt
    13261 atggcagttg cgtatggaag cggagagccg gatgttgttt acttcccagc agaaggtttc
    13321 ctgaccggag attatttaaa agaaaaattg actgagctgc agaagcagat tccggttttc
    13381 tgcgtcatgg acggaaaaga atttctgaag gatatgccgg acgccgatga agcccatctg
    13441 tttgatgccg gaattgccgc gtatttatta aatccattga aatcacagta ttcgtacgat
    13501 gatatcgtaa aagagtacgt acaccgctat gtgccggcag tggaagagat cttcggcgga
    13561 agcaagattc cggccgcagg aaagatgacg ccggaacagc aggagtcgta tgcaggtcat
    13621 caggcgtatg ctgtatttgc cgcacaggaa aacatggaaa agctgctgaa ggagcagggc
    13681 atgtgggatc tgtaccggaa tgtggagatt ccgctggtgt ttacgctccg tcagatggaa
    13741 gcggatggaa tcgccgcaga aaaagaagca ctttccgttt acggagctga gctggcggag
    13801 cggatcggag agctggaggc acagatctac gaggaggccg gcgaggagtt caacatcaat
    13861 tccccgaagc agctcggcgt gattctgttt gaaaagctgc agcttccggg cggcaaaaag
    13921 accaagacgg gttattccac cgccgccgat gtgctcgaaa agctcgcacc ggatcacaaa
    13981 ctcgttgccg atattctgga gtaccgccag cttgccaaac tgaaatcgac gtacgccgac
    14041 ggtctgcagg ccgtgatcgg gaaagatgga agaatccatt cgacatttaa ccagaccatc
    14101 accgcgaccg gtcgcatcag cagtaccgat ccgaatctgc agaacattcc ggtgcggatg
    14161 gagcttggcc gtctgatccg gaaagccttc gtgccaaagc ctggctgtgt ctttctggat
    14221 gccgactatt cccagattga gctccgcgtg ctggcccata tgtcgggcga tgaaaacctg
    14281 atcgaggcgt accgcgaggc ggaggatatc caccgcatga cggcatcgaa agtgttccat
    14341 gtgccgcttg aagaggtaac accgctgcag agaagaaatg cgaaggcggt caacttcggt
    14401 atcgtatacg gcatcagttc gttcggtctg ggccaggatc tcggcatcag ccggaaagag
    14461 gcagaggcct atatccagca gtatttcgag acgtatccgg gtatccatga gtttttgaac
    14521 cgctgcgtag aggatgccaa agagaaaggc tattccgtga cgatgttcgg acgcagacgg
    14581 ccgatgccgg agctgaaatc gagcaacttt atgcagcggt cattcggcga gcgcgttgcg
    14641 atgaatgcgc cgatccaggg aacggcggcg gatattatta aaatagcgat gatccgcgtg
    14701 gcgaaacggc tgaaagagga gcagttaaag accagacttc ttcttcaggt acacgatgag
    14761 ctgctgctgg aagtgccgga agacgagctc gaaaaggcag aacagatcct ggaagaggag
    14821 atgaccgggg cagcacatct ggctgtcaaa ctcgagatcg atatgcacac aggaaacaac
    14881 tggtacgagg cgaaatagcg cactcatacc aggagcgaaa ggatacaaaa gaatgaaagt
    14941 ttttggaatt acaggcggcg tgggcgccgg aaaaagcgaa gtgctcacgt accttggaca
    15001 gaattatgat gcaacggtga tccaggcgga cgaggcaggt taccttgtga tgctgccggg
    15061 cggcgaatgc tatggagaga ttgtgaagct gttcggcaga cagatcacca cagagaccgg
    15121 cgaacttgac cggaaacgga ttgctgagat cgtttttcag gacgaagaaa agttaaaagc
    15181 cctgaatgcg atcgttcatc cggcggtaaa acggtatatc aaaaaagcca ttgcagccgc
    15241 ggaaaaagca gggacggaat acgtctttgt ggaggccgca ctcttaatcg aagaaaaata
    15301 tgatgaaatt tgtgatgaac tgtggtatat ttatgcggat gaagaagtcc ggaaggagcg
    15361 attgatcgaa ggacgcggct attccgagaa gaaagttcgc gaaatcatgg caaatcagct
    15421 ttccgaggat gaattcagca gccactgcga ttttgaaatc gacaacagcg gcgattttga
    15481 ggaaacaaaa aaacagttag accataggat gaaaaaatat gaaactttgt agtattgcaa
    15541 gtggttcgag cggaaactgc attttcgcgg gaagcgagac cacaagcctt ctgatcgatg
    15601 cgggcatcag cggaaagcgc gtggaggcgg gactggctgt tctcgaccgc agcgcgaagg
    15661 agctggacgg cattctcgtg acacatgagc attccgatca tatccagggc cttggcgtgc
    15721 tggcgcgccg gtatggtatt ccgatttacg caacggatgg aacaattcag gcgatgcagc
    15781 agacgaagag ccttggaaag ttcccggagg ggattttcca cacgattcac gcggatgaca
    15841 cgtttacgct gggtgatatc gccgtacatc cgtttacgat ctcccacgat gcggcagagc
    15901 cggtcggcta ccgcatggaa tccggcggga aatcgattgc gatcgcgacg gaccttggtg
    15961 tctacagcga ttacacggtt cagaatctga ccgggctgga tgccgttttg ttagaggcaa
    16021 atcatgacga gaacatgctt gcggtcggac cgtacccgta tccgttaaaa cagcggatcg
    16081 ccggggcgaa aggccattta tcgaacctgg cggcaggcca tctcctctgc gagattctgc
    16141 acgacaatct gcaggaagtg ctgctcggcc atctgagcaa ggaaaataat tatgaggcac
    16201 tggcctttga aaccgtggcc tgcgaggtca caacgggtga taatccgtat cactcgggtg
    16261 attttcatct cgctgtcgct aaaagaagcg aagtatcacc gttaattgag gtttaaatat
    16321 acatttttag agctacatac catgtttttc gcaaggccgc cattaccggt ttttccgggg
    16381 ctttgacata aaaccagaaa aaggagaatg gaaacatgaa gaagacaatt atcactgtcg
    16441 taggcaagga caccgtgggc attatcgcaa aggtatgtaa ttatctggca gagaaccagg
    16501 taaacatcga agacatttct cagacgattg tgcagggata tttcaacatg atgatggtaa
    16561 cggacgtctc caagagcgag aaatccaacg cagagcttgc aaaagatctg gaggagcttg
    16621 gaagttccat cggcgttgtc atccgctgcc agcatgaaga tattttcaat atgatgcacc
    16681 gtatctaaag cgaaggagaa aaacgacatg ataaatctgt ttgaagtaaa ggaaacgaac
    16741 gagatgatcg aaaaagagaa tctggacgtt cgtaccatta cccttggaat cagtcttatg
    16801 gactgtatcg actccaatct tgacaagctg aaccgcaaaa tctacgacaa aatcacgacg
    16861 actgccagaa atctggtttc cgtaggtgag gagatcgagg gcgagttcgg tattccgatt
    16921 gtaaacaaga gaatttccgt taccccgatt gccttagtcg gcgcagcagc ctgcagaacg
    16981 ccggaggatt tcgtgacgat tgcacagact ctggaccgtg cggcaaaaga agtcggcgtc
    17041 aatttcctcg gcggctattc cgctctggta agcaaaggta tgacgacagc agatgagctt
    17101 ctgatccgtt ctatcccgca ggcactggct gttacggatt ttgtctgcag ctccatcaac
    17161 cttggctcta ccaaaaccgg tatcaacatg gatgctgtca aactgatggg tgagatcatc
    17221 aagaagacgg cggaggcaag caaagagaac aactgccttg gatgcgcaaa actcgttgtt
    17281 ttctgcaatg caccggacga caacccgttc atggcaggcg ctttccacgg cgtaaccgag
    17341 gcggatgcca tcatcaatgt cggcgtttcc ggccctggtg ttgtcaaaca tgctctggaa
    17401 aaagtacgcg gcgagaactt tgaggtactc tgcgagacga tcaagaagac cgcatttaag
    17461 gtaacgcgtg tcggccagct cgttgcacag gaggcttcca agcggctgaa cattccgttt
    17521 ggtatcattg acctttccct ggcaccgact ccggcgatcg gagattccgt tgcggatatc
    17581 cttgaggaga tcggcctcga gcacgcaggt gcaccgggaa cgacagcagc gctggcactt
    17641 ctgaacgatc aggtgaaaaa gggcggtgtt atggcatctt cctatgtcgg cggtctctcc
    17701 ggcgcattca tcccggtttc tgaggaccag ggtatgatca acgcagtcaa cgacggtgcg
    17761 ttgaccatcg agaagctgga agctatgact tgtgtctgct ctgtaggcct tgacatgatc
    17821 gccatcccgg gagacacgaa agcatcgacc atctccggaa tcatcgcaga tgagcttgcc
    17881 atcggtatgg tcaaccagaa aacaacggca gtccgcctga tcccggtcat cggaaaaggc
    17941 gtgggcgaga ccgtagaatt cggcggcctc ctcggctatg caccgatcat gccggtcaac
    18001 aacttctcct gcgaggcatt cgtcaacaga acaggaagaa tcccggcacc gattcattcg
    18061 tttaaaaact aaagagaaag ctgaaaagcc ggcagtgatc ctgaatggaa aggatcgctg
    18121 ccggcttttt taccatctgc gcaaataaaa atagtaattt taaggaatgt atagtatgat
    18181 atattacata ctgataattt tacttgcaac ccataaaaag tgtaagaaaa aatgaattat
    18241 tacggggaac ttcagcggta gcagccgttg ttctatagca attcaataaa ttattgcaca
    18301 taattacacg aatgaaacca ccctatgcag tccgttgga
//
LOCUS       DAOMUF010000021        18157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_576_length_18157_cov_3.937355, whole genome shotgun sequence.
ACCESSION   DAOMUF010000021 DAOMUF010000000
VERSION     DAOMUF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 18157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18157
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_576_length_18157_cov_3.937355"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1020
                     /locus_tag="PLN77_02585"
     CDS             181..1020
                     /locus_tag="PLN77_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRM43203.1"
                     /translation="MEKIIAGLVQMDSGPDMNENMRMAEQYVREAAEQGAKLILFPET
                     AEYTGADMPGHASAVPGYVSRRFSSLAKRYGVYLHCGSMTVRRHPGRPSNTSFLFGPD
                     GETIAEYSKIHMFDVNIPGSVSYEESHEICPGNEIVLADTALGLFGMSICYDIRFPEQ
                     YRLMASSGADAFLIAADFTKATGERHWEALLRTRAIENGCYVLAANQCGQKARFEAYG
                     HSMILAPDGEILAEADDTPQVLIAELDPEVLERTRNEIPSLENRRDDLYRVSSGNVRI
                     YEE"
     gene            1044..2228
                     /locus_tag="PLN77_02590"
                     /pseudo
     CDS             1044..2228
                     /locus_tag="PLN77_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013282492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            2244..4061
                     /locus_tag="PLN77_02595"
     CDS             2244..4061
                     /locus_tag="PLN77_02595"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM43204.1"
                     /translation="MAKKKIAALLIAAAVAAGGWALPARVSAAENDGTAVTWQQDETE
                     SSEQNSDEEAAVQEQSGTSENETPAVTIPGEDNEQEPEEKLPAGWVKQEDGSWKYRKE
                     DGTMAASEWITHLNRRYYLNADEIMCTGLSMVNGKLYYFESWGGAGYKGWKKVGGAWY
                     YLNEDGSVKTNEWFVHDKRTYHVDENGVMSTGLQKIDGTLYYFESWGGAGFQGWKKVG
                     GTWYYLNEDGSLRTNQWFVHDKRTYHVDADGVMSTGWQTIDGVDYYFESWGGMRVNAW
                     AAKGSDWYYMDSNGTPKGEGWLLYDKNWYYLRQDGKMMHSEWLWYDNNWYYLQSWGGM
                     YKSQWVTIKGATYYFRSWGGIYKNGWQEVDGKTYYFRSWGGIYKDTYIDGYYVDKNGV
                     RRNSKNICVAIDAGHQRRGNSEKEPIGPGSSTYKAKVASGTCGVATGINEYELNLAVS
                     LKVRDILEERGYDVYMIRETHDVNISNSERAKLAAQNGADILVRVHANGDSNQSVYGA
                     LTMAPSSRNTYLSGDVVSKSQKLSQKMIAAFCKDTGAKNRDVIYTDSMSGINWSTIPV
                     TIIELGFMSNPSEDRLMATEDYRNKMAQGIADGIDAYYE"
     gene            complement(4159..5391)
                     /locus_tag="PLN77_02600"
     CDS             complement(4159..5391)
                     /locus_tag="PLN77_02600"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRM43205.1"
                     /translation="MMKEKVVLAYSGGLDTTAIIPWLKETFDYEVICCCIDCGQGNEL
                     VGLDERAKLSGASKLYIENVIDEFSEDFIMPCVKAGAVYENKYLLGTSMARPVIAKKL
                     VEIARKEGAVAICHGATGKGNDQIRFELGIKALAPDIKVIAPWRMTDVWTMQSREDEL
                     AYCKAHGIDLPFDANHSYSRDRNLWHISHEGLELEDPSNEPNYDDLLVLTTPPEKAPD
                     EGEYVTMTFEAGVPKTLNGKEMKVSEIITELNKLGGKHGIGIVDIVENRVVGMKSRGV
                     YETPGGTILMAAHEQLEELILDRETLKTKKDLGNLLAEVVYEGKWYTPLREAIQAFVD
                     VTQKYVTGEVKFKLFKGNIIKAGESSPYSLYNESLASFTTGDLYDHHDADGFITLFGL
                     PLKVRAMKMQELEKNNNK"
     gene            5674..6714
                     /gene="argC"
                     /locus_tag="PLN77_02605"
     CDS             5674..6714
                     /gene="argC"
                     /locus_tag="PLN77_02605"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRM43206.1"
                     /translation="MIRAGIIGSTGYAGGELVRILLGHKDVEIKWYGSRSYIDKKYAS
                     VYQNMFQLVDDVCKDDNMEQLAKEVDVIFTATPQGLCASLVNDEILSKVKVIDLSADF
                     RIKDVKTYEEWYKIEHKSPQYIDEAVYGLCEINREKVKQARIVANPGCYPTCSTLSIY
                     PLLKEDLIDGNTIIIDAKSGTSGAGRGAKVDNLYCEVNENIKAYGVAGHRHTPEIEEQ
                     LSYAAGYPITLNFTPHLVPMNRGILVTAYASLKKDVTYEEVKAVYDKYYANEQFVRVL
                     DKDVCPQTKWVEGSNYVDVNFKIDPRTKRIIMMGAMDNLVKGAAGQAVQNMNLMFGLP
                     ENEGLCMPPMFP"
     gene            6767..7990
                     /gene="argJ"
                     /locus_tag="PLN77_02610"
     CDS             6767..7990
                     /gene="argJ"
                     /locus_tag="PLN77_02610"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRM43207.1"
                     /translation="MKKIEGGITAAKGFQAAGGAAGIKKQGVKDMALVYSEVPCVAAG
                     TFTTNIVKAAPVKWDQEIVYNHPTAQAIVCNSGIANACTGEEGYGYCRKTAEAASAAL
                     SIPEDSVLVASTGVIGKQIPIDKIAAGVEMLKPQLAATREAAATAAQAIMTTDTEPKE
                     VAVQIEIGGKTVTIGSMCKGSGMIHPNMCTMLSFVATDANISKEMLQKALSEVVPDTY
                     NMVSVDGDTSTNDTVLLLANGLAGNPEITEENEDYKIFKEALFEVNKTQAMHIAGDGE
                     GATALFEVTIQGAESKEQAVTLAKSVITSSLTKAAIFGHDANWGRILCAMGYSGAKFD
                     PENVDLYFESAAGKLQIITKGVAVDYSEEEATKILSEPKVTAIADIHMGEATATAWGC
                     DLTYDYVKINADYRS"
     gene            8098..8994
                     /gene="argB"
                     /locus_tag="PLN77_02615"
     CDS             8098..8994
                     /gene="argB"
                     /locus_tag="PLN77_02615"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRM43208.1"
                     /translation="MVNQMYLDKANVLIEALPYIQRFNRKIIVIKYGGSAMVDEELKR
                     NVIQDVVLLKLVGFKPIIVHGGGKEISRWVGKVGMEPKFVNGLRVTDADTMEVAEMVL
                     NKVNKELVTMIQSLGVSAVGISGKDGGLLNVEKKLSKGEDIGFVGNIKNVNPKVLYDL
                     LENDFLPVVCPVGMDDDFNTYNINADDAACAIAEALNAEKLAFLTDIEGVYKDPKDPE
                     SLISELHVQEARDLITNGNVGGGMIPKLQGCIDAIENGVSRVHIMDGRIPHCLLLEIF
                     TNKGIGTAILGENKEKFNHEDE"
     gene            8981..10171
                     /locus_tag="PLN77_02620"
     CDS             8981..10171
                     /locus_tag="PLN77_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRM43209.1"
                     /translation="MKTNEYMEQTEQYVLHTYNRFPLVIDHGEGVHLYDTDGKAYLDF
                     AAGIAVYALGYSNDDYKKAVKDQVDKVIHVSNLFYNVPMGSAAEKLAKASGMDKVFFT
                     NSGTESIEGAIKVARKYAYLKDGSNDHEIIAMNHSFHGRSLGALSVTGNTHYQDPFKP
                     LIGGIKFADFNDLESVKAQITDKTCAIIMETVQGEGGIYPATKEFLQGVRDLCDEHDI
                     LLILDEIQCGMGRTGKLFAWQDYGVQPDVMTCAKALGCGVPVGAFVLNKKTAEHSLVP
                     GDHGTTYGGNPLACAAVNAVFECYEKQHVVEHVAETAPYLEKKLDELVEKYDCLAARR
                     GKGFMQGLVVTGCPVGEVINRAIENGLLVISAGSDVLRMVPPLVITKDDIDEMIEKLE
                     KALQ"
     gene            10464..10895
                     /locus_tag="PLN77_02625"
     CDS             10464..10895
                     /locus_tag="PLN77_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM43210.1"
                     /translation="MAGILTALLSGALMSIQGVFNTEVTKQTSTWLAAGWVQISAFAV
                     CLAAWCITGREPIGDLFRVKPWYLLLGGAIGAFITITVIWSMAGLGPAKAAMLIVISQ
                     LAAAWLIELFGLFGMEKTDFTVRKLLGMAVAVVGIVVFQWE"
     gene            11009..11713
                     /locus_tag="PLN77_02630"
     CDS             11009..11713
                     /locus_tag="PLN77_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRM43211.1"
                     /translation="MKLKKQQNRGRKHAKILAAMLVFLLCGCNHRDTEYKLSDSTGGS
                     TAETVFGNQKEVDEEQTVSEKSAGETTGIPEKREAQEAAPEETVQVYICGAVASPGVY
                     TLPGGSRVVQAVEAAGGFLPDAEEKILNLARKIEDGEQITVWTREEAENMESTETPQQ
                     NTGGTEQASGSGKVNLNTAGKEELMTLSGIGESRADAIIAYREANGPFGSIEEIMNIE
                     GIKEKMFEKIRGSIEV"
     gene            11731..12420
                     /locus_tag="PLN77_02635"
     CDS             11731..12420
                     /locus_tag="PLN77_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM43212.1"
                     /translation="MAKKVLVVDDEKLIVKGIRFSLEQDGMEVDCAYDGEEALSMAKE
                     KEYDMILLDLMLPKLDGLSVCQQIREFSNVPIVMLTAKGEDMDKILGLEYGADDYITK
                     PFNILEVKARIKAIMRRAARQEPKEEKAKVVQVKDLRIDCEGRRVFIGDREINLTAKE
                     FDVLELLVFNPNKVYSRENLLNIVWGYEYPGDVRTVDVHIRRLREKIEKNPSEPKYVH
                     TKWGVGYYFQA"
     gene            12398..13843
                     /locus_tag="PLN77_02640"
     CDS             12398..13843
                     /locus_tag="PLN77_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM43213.1"
                     /translation="MVTISRLKIKEKWKFLRSLRFRITVILVIIGIVPCVILEKSIVN
                     SYEDRAVSVRSITVKNQCDIICNQLIQQNYMEDPGSDVINGQLSMLTNIYGGRILIID
                     QDFRVIKDTYDMDRGKTIISQEVIQSFQGKETSQYDRKNSYLEMTVRIQNTETKETLG
                     VMLVSISTDEIRANMKILETKGMTILSIIALLVLVFGYLLAGILMKPFQKVTRSIEDI
                     TDGYLDESISVPDYLETELITDAFNKMLTRVRTLDDSRQEFVSNVSHELKTPLASMKV
                     LADSLNGQEGVPVELYQEFMQDITSEIDRENQIITDLLSLVKMDKKVADLNITQVNIN
                     ELLEQLLKRLHPIAAQKNVELILDSFRPVNAEVDATKLSLAFSNLIENGIKYNNENGW
                     VRVSLNADHKYFYVTVADSGIGIPEESIAHIFERFYRVDKSHSREIGGTGLGLAIARS
                     AIVMHRGAIRVYSKENEGTTFSVRIPLNYVM"
     gene            13856..14779
                     /locus_tag="PLN77_02645"
     CDS             13856..14779
                     /locus_tag="PLN77_02645"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRM43214.1"
                     /translation="MKRILGVLLAGVLLLSGCGQWQKSQKESAYHLYCLDKTGTTLVQ
                     EPYEPKEGTDQKLIKELVDALQEKPARDGNQALLSNGISIKTYTLENGLLTLNLSGEY
                     GELARPQEVLVRAGLVRTFVQVPEVERVTLLIDGDPLKDSKGREIGTMTADSFLENSG
                     REIYQYQYATLTLYFANEAGDHLVKETRRVPYSTNIPLERVVVEQLMKGPKEDGHYAV
                     LPDTMNVLSVTTSDRIAYVNFDKSFKDSALSSVAEQIPIYAVVDSITANCKVDKVQFS
                     INGESDVTFRETMKLNQFYEEDLSYLEEKDT"
     gene            14776..17049
                     /locus_tag="PLN77_02650"
     CDS             14776..17049
                     /locus_tag="PLN77_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRM43215.1"
                     /translation="MRKRPMCLACLGFMLVLVLLRIAGVPLGTPFSDPKYENKAQQGR
                     KVWIAGTIETYEKKSSNNGYVLRGEGSKGKIYLLAKRGDLPVGAAVRVYGTVKKPESP
                     RNPGMFDEKTYYEGKGCAFYVISEREEVVDVGRWNLGEALRLERERCCAVLKEMMPEE
                     EAGVLSAMLLGERSELTEETYLAYQQSGLLHLISVSGMHLMLLGMALRRLLMALHLPK
                     TPASLAAAAGMVLYGMFTGSGTATVRAVVMFAVRMGAVAVGRTYDSLSALSLAAILML
                     AENPSYLEQSGFWLSFGAVTAISGVYPVLAGEKAERKRRGEKTGLEKLAAKGKEAAMV
                     YLSLQLVMIPLQAWYFYEIPLFAFLPNLFAGAVMTAVLLTGAAGLLAGLASVKAGSVV
                     LLPARFLLTLLRQMTAAVAQIPGNVWICGQPEKWQMAGYGIILTGLLVILYVRKRKRE
                     MNPDGAKRKGAQARRRERMLFGITMFFACVLLFYRPRETFSITALDVGQGDALVVESG
                     RFLMLNDGGSSSASAIGEKRILPYLKQRGIAALDAVVVTHPDADHTNGILELLELIGE
                     QKTALRIRHLFLPVWMKGSEKENPFILAAQKAGIMVEYLKKGDEIRAGELTVSVLHPG
                     AGEDYTGEENAGSVVLQLSCGACRALLTGDLEGSGEEEVLGAAERCQILKVAHHGSRN
                     STSEAFLNRIQPQISLISCAWPGRYGHPHRELLERLRACKSHIYGTPVDGAVTVQLKR
                     DRLAVHGYRADVEFPVS"
     gene            17121..18104
                     /gene="holA"
                     /locus_tag="PLN77_02655"
     CDS             17121..18104
                     /gene="holA"
                     /locus_tag="PLN77_02655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRM43216.1"
                     /translation="MKSILEDIKTQNFKQAYLLYGEEAYLKHQYKNKLKNALLPEDDT
                     MNFSRFEGKGTEIPKVIDLAETMPFFADRRVILLENTGFFKNKADALADYMGSLPDYL
                     VLIFVEEEVDKRNRMYKAVQKQGRAVEFARQDEKTLMTWVLGMMKKEGKKITRQDMED
                     FLEKTGTDMGNISQELEKLLSYTMGRDVITRADIEAVCTTQITNRIFEMIRAVTEKKQ
                     RKALDLYQDLLALKEPPMRILFLLARQFNLLLQVKELLAEHCDQATIAKRTGLQSFVV
                     RNYIPMSRQYAAEELKAAVNDCVQMEEDVKTGKISDLLSVELLITKYSRAA"
BASE COUNT         4968 a         4114 c         5289 g         3786 t
ORIGIN      
        1 gctgtttcgc tgcgtaagaa agtgattcag gtgtgagcag ccctcctttt gcgtagcaaa
       61 actttcaatg agggctgcga atgttacagt tcgcaggtat ctgtgaactg taacgatata
      121 ataaacgaga gtataagcgg aaaaatcaca agctaacatg tgaaacgagg ggacaaaaag
      181 atggaaaaaa taattgcagg cctggtgcag atggattccg ggccggatat gaatgaaaat
      241 atgcgtatgg ccgagcagta tgtgcgggag gcggcggaac agggagcgaa gctgattctt
      301 ttcccggaga ccgcagaata cacgggagca gacatgccgg gccacgcaag tgcagtgccg
      361 ggctacgtca gccgccggtt ttccagcctt gccaaacggt acggtgtcta tctgcactgc
      421 ggcagcatga cggtacggcg ccatccgggg cgtccgtcca acacgtcgtt tctgttcgga
      481 ccagatggag aaacgattgc agaatacagc aaaatacata tgtttgatgt aaatattccg
      541 ggcagtgtct cctacgaaga atcacacgag atctgcccgg gaaatgaaat tgttcttgcg
      601 gacacggcgc ttggcctgtt cggcatgagc atctgctacg atatccgctt tccggaacag
      661 taccggctga tggcatcctc cggagcagat gcatttctga tagcggcgga ttttacgaaa
      721 gcaacgggag agcgccactg ggaagcactg ctgcgcacgc gcgccattga aaacggatgc
      781 tacgtgctcg ccgccaacca gtgcggccag aaagcgcggt ttgaggcgta cggccattcc
      841 atgatccttg cgccggacgg cgaaattctt gcggaggcag atgatacccc gcaggtactc
      901 atcgcagagc tggatccgga agtgctggag cggacaagaa atgaaatccc gtctctggaa
      961 aacaggagag atgatcttta ccgtgtaagc tcgggaaacg tacggattta tgaagaataa
     1021 aaatgcaggg aggaaataag aagatggatc agtttataca gtataccccg acggaagtcg
     1081 tgttcggaaa agagaccgaa aaccaggcag gcgctgaggt aaagaaatgg ggcggcacaa
     1141 aggtgctcgt cgtttacgga ggcggaagtg caaaacggag cggtcttctg gaccgagtgg
     1201 aagcatctct tgcaaaagag ggccttgcct ttgaagagct tggcggcgta aaaccaaacc
     1261 cgagacttgc ccttgccgag gagggtgtga aaaaagcagt ggaaatgcag gcagacttta
     1321 ttcttgccgt cggcggcgga agctcgattg acaccgcaaa agccgttgcg catggagctg
     1381 caaatccggc ggaggcactc tgggatatct ggacgaaaaa agtaccgctg acaaaatctc
     1441 tcccggtagg agcggttctg acgataccgg cagccggaag cgagatgagc gattcagccg
     1501 ttctgacaaa cgaggcaatc ggcaaaaagg ccggcatcaa cacgccgttc aaccgctgct
     1561 aatttgccat catgaatccg gagctggcag caaccctgcc gaaataccag attgcctgcg
     1621 gagtaaccga catcatgatg cacacgatgg agcgctattt cattccggag agccagtgcg
     1681 agatgacgga cgagattgca gagggactcc tccgtaccgt aatcaaaaac ggaccgaagg
     1741 ttctggaaaa cccatccgat tacaatgcga tggcggaaat catgtggtgc ggaagcctgt
     1801 cacacaatgg actgacggag tgcggacgcg gcaaagattt ttcggtacat aaattcggcc
     1861 atgcgttaag tgcaaaatat gatgtagcac acggcgcatc cctggcggca gtctggggtg
     1921 cctgggcgaa ataccagtac gatgaggaga cggttctctg gcggtttgca cggtttgcag
     1981 aaaaagtctg gggcatcaca gagggaagcg atcacaaacg ggcaacggaa ggcattgagc
     2041 gcacagtagc gttcttccat tcgctcggca tgccgacctc cctgcatgag cttgatatcc
     2101 cgaacccgtc ggatgaggat ctgcgtgccc tttccatgga tgcaacacag caggacactg
     2161 taaaactgtc ccgcatccgc aagctgggcg cacaggaagt atatgagatt taccagatgg
     2221 cgcgataaaa ggaggaaaac cagatggcaa aaaagaaaat cgcagcactg ctgatcgcag
     2281 cggccgttgc ggcaggaggc tgggcgcttc cggcaagggt aagcgcagca gaaaatgacg
     2341 gcacggccgt aacctggcag caggatgaga cggaaagttc cgagcagaat tcggatgagg
     2401 aagcggcagt gcaggagcag agcggtactt cggaaaacga gacgccggct gttacgattc
     2461 caggtgagga caacgaacag gagccggaag agaaacttcc tgccggctgg gtgaaacagg
     2521 aggacggaag ctggaaatac agaaaagagg acggcacgat ggccgccagc gagtggatca
     2581 cccatctgaa ccggagatat tatctgaatg cagacgaaat catgtgcacg ggtctttcga
     2641 tggtcaacgg aaagctctat tattttgaga gctggggcgg agccggatac aaaggctgga
     2701 aaaaagtcgg cggggcatgg tactatctga acgaggacgg aagcgtcaaa acaaatgaat
     2761 ggttcgttca cgacaagaga acataccatg tagatgaaaa cggtgtgatg agcaccggac
     2821 tgcagaagat tgacggaacg ctctactact tcgagagctg gggcggagcc ggattccagg
     2881 gctggaaaaa agtcggcgga acatggtact atctgaatga ggacggaagc ctcagaacga
     2941 atcagtggtt tgtgcatgat aaaagaacgt accatgtaga cgcagacggt gtgatgagta
     3001 ccggctggca gacgattgac ggcgttgatt attactttga aagctggggc ggcatgcgcg
     3061 tgaacgcctg ggcggccaag ggcagtgact ggtattacat ggacagcaac ggaaccccaa
     3121 aaggggaagg ctggctgctg tatgacaaga actggtatta tctgaggcag gacggcaaaa
     3181 tgatgcacag tgaatggctg tggtatgata ataactggta ttacctgcag agctggggcg
     3241 gcatgtataa gagccagtgg gtaacgatca aaggcgcaac gtattacttc aggagctggg
     3301 gcggtattta caagaacggc tggcaggaag tggacggaaa aacgtattac ttccgaagct
     3361 ggggaggcat ctataaggat acctacatcg acggctatta cgttgataaa aacggtgtcc
     3421 gccggaacag caaaaatatc tgcgtggcaa tcgacgcagg acaccagaga agaggaaaca
     3481 gcgagaaaga gccgatcgga ccgggatcat cgacctataa ggcgaaggtt gcttccggaa
     3541 catgcggtgt ggcaaccggc atcaatgagt atgagctgaa ccttgcggta tccttaaagg
     3601 tacgcgatat ccttgaggag cgcggctacg atgtttacat gatccgcgag acgcacgacg
     3661 tcaatatcag caactccgag cgtgcaaaac ttgccgcgca gaacggagca gacattctcg
     3721 tgcgtgtaca cgcgaacggc gattcgaacc agtcggttta cggagcactc acgatggcac
     3781 cgagcagcag aaatacgtat ctttccggtg atgtggtcag caagagccag aagctctccc
     3841 agaagatgat cgcagcattc tgcaaagaca ccggtgcgaa gaaccgcgat gtgatttaca
     3901 ccgattccat gagcggcatc aactggagta ccattccggt cacgatcatt gagcttggct
     3961 tcatgagcaa tccttccgag gaccgtttga tggcaaccga ggattaccga aacaagatgg
     4021 cacagggaat tgcggacgga atcgacgcat attacgaata aacaaaggga cctgcctgaa
     4081 atgggcgggt cctttttgca tgcaaaaaaa gaaagcggag cgagtccgct ttcttttggg
     4141 aatccatagg atcagatctt atttgttgtt gtttttctcc agctcctgca ttttcatcgc
     4201 acgaaccttc agcggcagac cgaacagtgt gatgaatccg tctgcatcgt ggtgatcgta
     4261 gagatcgccg gtggtgaagg aagcaagaga ctcattgtac aggctgtacg gagaagactc
     4321 gccggctttg atgatgttgc ctttgaacag tttgaatttt acttcgcctg ttacgtattt
     4381 ctgggttaca tcaacgaatg cctggattgc ctcgcgcaga ggagtgtacc attttccttc
     4441 gtaaacaacc tcagcaagca ggtttccgag atctttcttt gttttcaggg tctcacggtc
     4501 gagaatcagc tcctctaact gctcatgtgc agccatcagg atagttccgc caggagtctc
     4561 ataaacaccg cgggatttca tgccgacaac acggttctca acgatatcaa caataccgat
     4621 gccgtgtttt ccacccagct tgttcagctc ggtgatgatt tcagaaactt tcatttcttt
     4681 tccgtttaag gtcttcggaa cgccggcctc aaaggtcatg gttacgtact cgccttcgtc
     4741 cggagctttt tccggaggag tggtcagtac gagaagatcg tcgtagttcg gctcgttgga
     4801 cggatcctca agctcaagtc cctcatggct gatgtgccac aggttacggt cacggctgta
     4861 gctgtggttt gcatcaaacg gaaggtcgat gccgtgtgct ttacagtaag ccagctcgtc
     4921 ttcacgggac tgcatggtcc atacatcggt cattctccaa ggagcgatta ctttgatatc
     4981 cggagcaagg gctttgatgc cgagctcgaa acggatctgg tcatttcctt taccggtagc
     5041 gccgtggcag atggcaacag caccttcttt tcttgcgatc tcaacgagtt tctttgcgat
     5101 aaccggacgt gccatggaag taccgagcag gtatttgttc tcataaacag cgcctgcttt
     5161 tacgcacggc atgatgaagt cttcactgaa ctcgtcgata acgttttcga tatataattt
     5221 ggaagcgccg gaaagttttg ctctctcatc gaggccaacc agctcgtttc cctggccgca
     5281 gtcgatgcag cagcagatta cttcgtagtc aaaggtctct ttcagccacg gaataatggc
     5341 agtagtatca agtccgccgg aataggcaag tacaactttt tctttcataa tgtaagatcc
     5401 tccttggatt tctcgtgcac ctatatgcgc ggtgcatcgt caatgactaa aatatacatc
     5461 atacgacagg attatgcaag ctttttttca gaaattcaaa aaaaacttgc ataatataca
     5521 tttgaggtgt atacttatgc aatcgacagt atatgacagc agaaaacaaa gatttttcat
     5581 tgaaaaaaga aaataattga gctatcataa agaaaaaaga aaaaatgtcc ggcaagacaa
     5641 gaaaacgggc gaaagaaaaa aggagaaaaa ggtatgatca gagcaggaat tatcggttct
     5701 acaggatatg caggaggcga gctggtgcgt attctcctgg gacacaaaga tgtggagatt
     5761 aaatggtatg gttccagaag ctatatcgat aagaaatatg cgtcggttta ccagaatatg
     5821 tttcagttag tagacgatgt ctgcaaggac gacaacatgg agcagcttgc aaaggaagta
     5881 gacgtcattt ttacagcaac tccgcaggga ctctgtgctt ccctggtaaa tgatgaaatt
     5941 ctttcgaaag taaaggtcat cgacctgagc gccgatttcc gtattaaaga tgtaaaaacg
     6001 tatgaagagt ggtacaagat cgagcataaa tccccgcagt atatcgacga ggcggtttac
     6061 ggactgtgcg agatcaaccg tgagaaagta aaacaggcgc gcatcgtggc aaacccggga
     6121 tgctatccga cctgctcaac tctttcgatt tatcctttat taaaggaaga tctcattgat
     6181 ggcaacacca tcatcatcga tgcaaaatcc ggaacttccg gagcaggaag aggagcgaaa
     6241 gtggacaacc tctactgcga ggttaatgaa aatatcaagg catacggcgt tgccggccac
     6301 cgccacacac cggagatcga ggagcagctt tcctatgcag ccggctaccc gatcaccctg
     6361 aacttcaccc cgcatcttgt tccgatgaac cgcggcattc tcgtaacggc atatgcatcc
     6421 ctgaaaaaag atgtgactta cgaagaggtt aaagccgttt atgataagta ttatgccaac
     6481 gagcagtttg tacgtgttct cgataaggat gtctgcccgc agacgaaatg ggtcgaggga
     6541 agcaactatg tggatgtcaa cttcaagatc gatccgagaa cgaaacgaat catcatgatg
     6601 ggtgcgatgg acaacctggt caaaggcgca gcaggacagg cggtacagaa tatgaacctg
     6661 atgttcggcc tgccggaaaa cgaaggcctg tgcatgccgc cgatgttccc gtaaaaaaga
     6721 cagaagcaga caacagacgg aatttacgaa aagcgaggat aaaagaatga aaaagattga
     6781 aggaggcatc actgctgcaa aaggttttca ggcagcaggc ggcgcagcag gaattaaaaa
     6841 acagggcgta aaggatatgg cacttgtcta cagcgaggta ccgtgcgtag cagcaggaac
     6901 ctttactaca aacatcgtaa aagcagctcc ggtcaaatgg gaccaggaga ttgtatacaa
     6961 ccatccaacc gcacaggcaa tcgtctgcaa cagcggaatt gccaatgcat gcaccggcga
     7021 ggagggctat ggctactgca gaaaaaccgc agaggcagca tcagctgcac tttccattcc
     7081 agaggactcc gttctcgttg catcgaccgg tgttatcgga aaacagatcc cgatcgataa
     7141 gatcgcagcc ggcgtggaaa tgctgaaacc gcagcttgca gctacaagag aagccgcagc
     7201 tacagcagca caggcgatca tgacaaccga caccgagccg aaggaagttg ctgttcagat
     7261 cgaaatcggc ggaaaaaccg taacgatcgg cagcatgtgc aagggctccg gcatgatcca
     7321 tccgaatatg tgcaccatgc tgagctttgt ggcaaccgat gccaatattt cgaaagagat
     7381 gctgcagaaa gcactgagcg aagtcgtacc ggacacctac aacatggttt ccgtagacgg
     7441 tgatacttcc acaaacgata ccgttctcct tctggcaaac ggtcttgccg gaaacccgga
     7501 aattacggaa gaaaacgagg attacaagat tttcaaagaa gcactctttg aagtcaataa
     7561 aacacaggcg atgcacatcg caggcgacgg cgagggcgca accgcactgt tcgaagtgac
     7621 gattcaggga gccgagagca aagagcaggc cgtgacactg gcaaaatccg tcatcacatc
     7681 gagtctgacg aaagcggcaa tcttcggcca tgacgccaac tggggaagaa tcctctgcgc
     7741 gatgggttac tccggagcaa aattcgatcc ggaaaatgta gacctctact ttgagagcgc
     7801 agccggaaaa ctgcagatta tcacgaaagg tgttgccgtt gactacagcg aggaggaggc
     7861 aacgaagatc ctctccgagc cgaaagtaac tgcgatcgcc gacatccaca tgggagaggc
     7921 gacagcaacg gcatggggct gcgacctgac ctatgactat gtaaaaatta atgcggatta
     7981 ccgttcttaa aaaacattcg gatgaaattc tgagggaata caacgaaaac gtgccgggaa
     8041 atcgtgtttc cggtacagca gaaccaggaa tcaaaagagg agagcgagga aaaaatcatg
     8101 gtaaatcaga tgtatctgga caaagcaaat gtgctgatcg aggcacttcc ttatattcag
     8161 cggttcaacc gcaaaatcat tgtcatcaaa tacggcggaa gcgcaatggt ggatgaagag
     8221 ttaaagagaa acgtcatcca ggacgtagtt cttctgaaac tggtcggctt caaaccgatc
     8281 atcgtacacg gcggcggaaa ggaaatcagc cgctgggtcg gaaaagtcgg catggaaccg
     8341 aaattcgtca acggccttcg tgtaacggat gccgatacga tggaagtggc agagatggtg
     8401 ctgaataagg tcaacaaaga gcttgtcacc atgattcagt cactcggagt tagtgctgtc
     8461 ggcatcagcg gaaaagacgg cggactctta aatgtagaga agaagctgtc caagggcgag
     8521 gatatcggct tcgttggaaa tatcaagaac gtaaacccga aggtgctgta cgatcttctg
     8581 gagaacgact tccttccggt tgtctgcccg gtaggcatgg atgatgattt caatacttac
     8641 aatatcaacg cagacgacgc ggcatgcgcc atcgctgagg cgttaaatgc agaaaaactg
     8701 gcattcctga ccgatatcga gggtgtctac aaagatccga aggatccgga gagcctgatt
     8761 tccgagctcc acgtacagga agcaagagat ctcatcacaa acggaaatgt cggcggcggc
     8821 atgattccaa aactgcaggg ctgcattgat gcgatcgaga acggcgtatc gagagtccac
     8881 atcatggacg gaagaatccc gcactgcctg ctgcttgaga tctttacgaa caaaggaatt
     8941 ggtacggcta ttttagggga aaacaaggag aaattcaatc atgaagacga atgagtatat
     9001 ggaacagacc gagcagtacg tgctccacac ctataaccgg ttcccgctcg tgatcgacca
     9061 cggcgagggc gttcatctgt atgacaccga cggaaaagca tatctggact ttgcggcagg
     9121 tatcgcggta tatgcgctcg gctacagcaa tgacgattat aaaaaagcag tgaaagacca
     9181 ggtggacaaa gtcatccatg tttccaacct gttctacaat gttccgatgg gctccgcggc
     9241 agaaaagctg gcgaaggcat ccggcatgga taaagtattc tttacgaaca gcggtacgga
     9301 gtcgatcgag ggagccatca aagtagcgag aaaatacgct tacttaaaag acggcagcaa
     9361 cgatcatgag atcatcgcga tgaaccattc cttccatgga agaagccttg gagcgctttc
     9421 tgtaacagga aatacgcatt atcaggatcc gttcaaaccg ctgatcggcg gcattaaatt
     9481 tgcggatttt aatgatctgg agagcgtaaa ggcacagatt accgacaaga catgtgcgat
     9541 catcatggag actgtacagg gcgagggcgg tatctatccg gcaacgaaag aatttttaca
     9601 gggcgtgcgc gatctgtgtg acgagcacga catcctgctg attctcgacg agatccagtg
     9661 cggtatggga agaacgggaa aactgttcgc atggcaggat tacggcgtac agccggatgt
     9721 catgacgtgt gcaaaagcac ttggctgcgg cgttccggtc ggcgccttcg tgctgaataa
     9781 aaagacggca gagcattcgc tcgttccggg agaccacgga acgacatacg gcggaaaccc
     9841 gctggcctgc gcggctgtca atgctgtatt tgaatgctat gagaaacagc atgtcgtaga
     9901 gcatgtagca gagacggctc cgtatctgga gaaaaagctg gacgaactgg tggaaaaata
     9961 tgactgcctc gcagcacgcc gtggaaaagg ctttatgcag ggccttgtcg taacgggctg
    10021 tccggtcgga gaagtcatca accgcgcgat tgagaacgga cttcttgtga tttccgccgg
    10081 aagcgacgtt cttcgtatgg taccgccgct tgtgattacg aaagatgaca tcgatgagat
    10141 gattgaaaaa ctggagaaag cattacagta atgtgaaata gacagccgtc tggtttccta
    10201 tgcaggagac cgggcggctg ttttcctgct gtccctgagt tctgtataaa aacggggaaa
    10261 aaaggaatag tataaaatgt actctcgggg cctttctgca cttgctttta cggccgattt
    10321 tccgccagct gcacccaaat ttcatcaacg tacgcaccgc gtacgcctca gaaatttggg
    10381 cacaactgac aaaaaatctt ctcgtaaaat caagcgcaga aagactccga gagtacataa
    10441 aaaaacagca ggaggaagaa tcaatggcgg gaattttaac agcgctgctt tccggcgcac
    10501 tgatgagtat tcagggcgtt ttcaacacag aggtgacgaa acagacgagt acatggctgg
    10561 cggccggctg ggtgcagatt agtgcgtttg ccgtctgtct tgcggcatgg tgcatcaccg
    10621 ggagggagcc catcggggat ctgttccgcg taaagccgtg gtatctgctg ctcggcgggg
    10681 caattggcgc gtttatcacg atcacggtta tctggagtat ggcagggctc ggcccggcga
    10741 aggcggcgat gctgatcgtc atttcccagc tcgcagcggc gtggctgatt gaactgttcg
    10801 gccttttcgg tatggaaaaa acggatttca ccgtgcgcaa gctgctcggc atggcagtgg
    10861 cagttgtggg aattgtagtg tttcagtggg aataacgatt aagatttcta cttgtatttt
    10921 tatggtttct tcgatacaat agaaacgtag ttatgcgtac acaagggacc cttgcgcgtc
    10981 agatgcgaag cggaagggag tacacagcat gaaattgaaa aaacagcaga acagaggcag
    11041 aaaacatgca aaaatactgg cggcgatgct ggtttttttg ctctgcggat gcaaccacag
    11101 ggatacagaa tacaaactgt cagatagcac aggcgggagt acggcggaaa ccgttttcgg
    11161 gaatcagaag gaagtggatg aggagcagac ggtttccgaa aaaagcgccg gagaaaccac
    11221 aggcattccg gaaaaacgtg aggcacagga ggcagcaccg gaagaaacgg tgcaggttta
    11281 tatctgcggt gcggtcgctt cccccggtgt ctacacgctg ccgggcggaa gccgcgtagt
    11341 gcaggctgtg gaagcggcag gcggatttct gccggatgct gaagaaaaaa tcctcaatct
    11401 tgcacggaaa atcgaagacg gagagcagat caccgtctgg acgcgcgagg aggcagaaaa
    11461 catggaaagc acggaaacgc cgcagcagaa tacgggcggc acggagcagg cttccggttc
    11521 cggcaaagtc aacctgaaca cggccggaaa agaagaactg atgacactct ccggcatcgg
    11581 agagagccgt gcggatgcca tcatcgccta ccgcgaagca aacggcccgt ttggttcgat
    11641 tgaggagatt atgaacattg agggcatcaa ggaaaagatg tttgaaaaga taaggggttc
    11701 tatcgaggtt tagaacaaga aggagaagaa atggcaaaga aagttcttgt agttgatgat
    11761 gaaaaactga tcgtaaaagg aatccgcttc agcctggagc aggacggcat ggaggttgac
    11821 tgcgcatacg acggcgagga agcactctcc atggcaaaag aaaaagaata cgatatgatc
    11881 cttctggatc tgatgctgcc aaagctggac ggattgtccg tctgccagca gatccgtgaa
    11941 ttttccaacg ttccgatcgt catgctgacc gcaaagggcg aggacatgga caaaatcctg
    12001 ggactggaat acggcgcaga tgattacatc acaaagccgt tcaacattct ggaagtaaaa
    12061 gcacgcatca aggccatcat gcgccgtgcg gcccgccagg agccaaaaga agagaaagca
    12121 aaagtcgtac aggtgaaaga cctgcggatc gactgcgagg gcagaagagt cttcatcgga
    12181 gaccgcgaga tcaatctgac tgcgaaggag ttcgatgtgc tcgagcttct cgtcttcaat
    12241 ccgaacaagg tgtacagcag agagaacctg ctgaacattg tctggggcta tgagtacccg
    12301 ggcgacgtgc gcaccgtaga tgtacatatc cgccgtctgc gcgaaaagat tgagaaaaat
    12361 ccaagtgaac cgaagtacgt tcacacaaag tggggagttg gttactattt ccaggcttaa
    12421 aatcaaagaa aaatggaagt ttctgcggag tctgcgcttc cgcatcacgg tgattcttgt
    12481 gatcatcggc attgtaccgt gtgtgatcct tgagaagagc atcgtaaaca gctatgagga
    12541 ccgtgcggta tcggtgcgca gcattaccgt aaaaaatcag tgcgatatta tctgcaacca
    12601 gctcatccag cagaattaca tggaagatcc tggatcggat gtcatcaacg gacagctcag
    12661 tatgctgacg aatatctacg gcggcagaat cctgattatt gaccaggatt tccgcgtcat
    12721 caaagatacg tacgatatgg accgcggaaa gacgattatc tcgcaggagg tcatccagag
    12781 ctttcagggc aaggagacga gccagtacga ccggaaaaac agctatctgg aaatgacggt
    12841 gcggattcag aataccgaga cgaaggagac gctcggcgtt atgctcgtca gcatttcgac
    12901 ggatgagatc cgtgcgaata tgaaaattct ggagacgaag ggaatgacga ttctttcgat
    12961 cattgcgctt ctcgtgctgg tttttggcta tctgctcgcg ggcattctga tgaagccgtt
    13021 ccagaaggtc acccgttcca tcgaggacat caccgacggc tatctcgacg aaagcatttc
    13081 tgttccggat tacctcgaga cagagctgat cacggacgca ttcaacaaga tgctgacccg
    13141 cgtccgcacg ctcgacgatt cccgccagga atttgtttcg aacgtctcgc atgagttaaa
    13201 gacgccgctt gcgtccatga aggttctggc ggattcgctg aacgggcagg agggcgttcc
    13261 ggtggaactt tatcaggagt tcatgcagga tattacctcg gagattgacc gcgaaaacca
    13321 gattattacc gaccttttgt cgctggtcaa gatggataaa aaggtggcag acctcaatat
    13381 cacccaggtc aatatcaacg agctgttaga gcagctgctg aagcggcttc atccgattgc
    13441 cgcacagaaa aatgtggagc tcattctcga cagcttccgc ccggtcaatg cggaggtgga
    13501 tgcgacgaag ctctcgctgg cgttctcgaa cctgatcgaa aacggcatca agtacaacaa
    13561 cgagaatgga tgggtgcgtg tatccttaaa tgcggatcac aaatatttct acgtgacggt
    13621 ggcagactcc ggcatcggca tcccggagga gtcgattgcg catatcttcg agcgtttcta
    13681 ccgcgtggac aagtcccact cgcgggaaat cggcggaacc ggccttggcc ttgcgattgc
    13741 gcggagcgcg attgtcatgc accggggagc gatccgtgta tacagcaaag aaaacgaggg
    13801 aacgacattt tccgtgcgga ttcctctgaa ttacgtgatg taggaggtgg cgcacatgaa
    13861 acggatactt ggcgtactgc tggccggcgt gctgcttctt agcggctgcg ggcagtggca
    13921 gaagagccag aaggagtcgg cgtatcacct gtactgcctg gacaaaaccg gcacaacact
    13981 ggtgcaggag ccgtatgaac caaaagaagg aacggatcag aaactgatca aggagctggt
    14041 tgacgcgctg caggagaaac ctgccaggga cggaaaccag gcacttcttt ccaatgggat
    14101 ttccatcaag acgtatacgc tggagaacgg gctccttacg ctgaacttaa gtggagagta
    14161 cggagagctt gcgcggccgc aggaggtttt ggtgcgtgca ggactggtgc gtacctttgt
    14221 gcaggttccg gaggtggagc gcgtgacgct tctgatcgac ggggatccgt taaaggattc
    14281 caaaggacgc gagatcggaa cgatgacggc ggacagcttt ctggaaaatt ccggaaggga
    14341 aatttatcag tatcagtatg cgacgctgac actgtatttt gcaaatgaag caggcgatca
    14401 tcttgtgaag gagacgcggc gggtgccgta cagcacgaac attccgctgg agcgtgtcgt
    14461 ggtggagcag ctgatgaagg gaccgaaaga agacggccac tatgcagtgc tgccggatac
    14521 gatgaatgtc ttaagtgtca cgacatccga tcggattgcc tatgtgaatt ttgacaaatc
    14581 ttttaaggat tctgcgcttt cgagtgtggc ggagcagatt ccgatttatg cggtggtgga
    14641 ttccatcacg gcgaactgta aggtggataa ggtgcagttt tccatcaacg gagagagcga
    14701 tgtaaccttc cgtgaaacga tgaaactcaa tcagttctac gaggaagacc tgtcttatct
    14761 ggaggaaaaa gatacatgag aaaacggccc atgtgcctgg cgtgtcttgg attcatgctg
    14821 gtgcttgtgc tgctgcggat tgcgggcgtg cctcttggca cgcctttttc tgaccccaaa
    14881 tacgaaaaca aagcgcagca gggcaggaag gtgtggattg cagggacaat tgaaacgtat
    14941 gagaaaaaga gcagcaacaa cgggtatgtg ctgcgcgggg agggaagcaa agggaaaatt
    15001 tatctgctcg caaagcgggg agatctgccg gtcggagccg ctgtgcgggt gtacggaact
    15061 gtaaaaaagc cggaaagccc gcgcaatccg gggatgtttg atgagaaaac atattatgag
    15121 ggaaaaggct gtgcgttcta cgtgatttcg gagcgggagg aagttgtgga tgtcgggcgg
    15181 tggaatctcg gcgaagcgct ccggctggag cgggagcggt gctgtgccgt tttaaaagaa
    15241 atgatgccgg aggaggaggc aggtgtgctt tctgcgatgc tgctcgggga acggtcggaa
    15301 ctcacggagg agacgtatct ggcgtatcag cagagcggtc tgctgcacct gatttccgtt
    15361 tccggcatgc atctgatgct gctcggcatg gcactccgac ggctgctgat ggcgctgcat
    15421 ctgccgaaaa ctcccgcgtc tctggcagcg gccgccggta tggtgctgta tggcatgttt
    15481 accggcagcg gaacggcgac cgtgcgtgca gtggtgatgt ttgcggtgcg gatgggagct
    15541 gtcgcagtgg gaaggacgta tgattcctta agtgcgctct ctcttgcggc aattctgatg
    15601 ctggcagaaa acccctcgta tctggagcag agcgggttct ggctttcgtt tggagcagta
    15661 acagccattt ccggggttta tccggttctg gcgggggaga aggcggaacg gaagagacgc
    15721 ggggaaaaaa cagggctgga aaagcttgcg gcgaaaggaa aagaggctgc gatggtgtac
    15781 ctttccctgc agcttgtcat gatcccgctg caggcgtggt atttttatga gattccgctc
    15841 tttgcgtttc tgccgaatct gtttgcagga gctgtcatga cggcggtgct gctgacgggt
    15901 gctgcggggc ttctggcggg actggcaagc gtgaaagcgg gaagtgttgt tctgcttccg
    15961 gcgcggttcc tgctcacact tctgcggcag atgacagcgg cggtcgcaca gattccggga
    16021 aatgtgtgga tctgcggtca gccggaaaaa tggcagatgg caggatatgg aatcattctg
    16081 accggtctgc tggtgatact gtatgtgcga aaacgaaaac gggagatgaa tccggatggg
    16141 gcaaaacgaa aaggtgcgca ggcacgaagg cgggagcgga tgctctttgg tattaccatg
    16201 ttttttgcct gtgttctgct tttttaccgt ccccgggaaa cgttttccat tacagcgctg
    16261 gacgtcggcc agggcgatgc gctggtggtg gagagcggcc gttttctgat gctgaatgac
    16321 ggcgggagca gcagtgcgtc tgcgatcggg gaaaaaagga ttctcccgta tctgaaacag
    16381 cgcggcattg ccgctcttga tgccgttgtt gtcacgcacc cggacgcaga tcacacgaac
    16441 ggaatcctcg aactgctgga gctgatcggg gagcagaaaa cggcgcttcg catccgccat
    16501 ctgttcctgc ctgtgtggat gaaaggaagc gaaaaagaaa atccttttat tttggccgca
    16561 caaaaagcgg gaattatggt agaatatcta aaaaagggag acgagatccg tgcaggagaa
    16621 ctcaccgtat cggtgcttca tccgggagcg ggcgaagact atacgggaga ggaaaacgcc
    16681 ggaagtgttg tattacagct ttcctgcggt gcctgccgcg cacttctgac gggagatctg
    16741 gagggaagcg gagaagaaga ggtcctgggg gcggcagagc ggtgccagat tttaaaggtg
    16801 gcgcaccacg gctcgagaaa ttcaacgagc gaggcatttc tgaaccgcat acagccgcag
    16861 atcagcctga tttcctgcgc gtggccggga cggtacggcc atccgcaccg ggagctgctg
    16921 gagcggcttc gtgcctgcaa aagccatatc tacggaacac cggtggatgg agccgtgaca
    16981 gtacagctga agcgtgaccg cctggcggtt cacggatacc gggcggatgt ggaatttccc
    17041 gtctcctgaa tggtcaaaag ctccggctgc tgcggcactg cgcaaacgtg cttctgccgg
    17101 aacgaaagaa agaggatgga atgaaaagca ttttagaaga tatcaaaacg cagaatttta
    17161 agcaggccta cctgctctac ggagaagaag cctatctgaa gcatcagtat aaaaacaagc
    17221 tgaaaaacgc actgctgccg gaggacgaca cgatgaattt ctcccgcttt gagggaaaag
    17281 gaaccgagat tcccaaagtg attgaccttg cggagacgat gccgtttttt gcggaccgcc
    17341 gtgtgattct gctggaaaat acaggatttt tcaaaaacaa agccgacgcg ctggcagact
    17401 acatgggatc cctgccggat tatctggtgc tgatcttcgt ggaggaggaa gtcgataagc
    17461 ggaaccgtat gtacaaggcg gtgcagaagc agggaagagc agtggaattt gctcgtcagg
    17521 acgaaaagac gctgatgacc tgggtactcg gcatgatgaa aaaagaggga aaaaagatca
    17581 cgcgccagga tatggaagat tttctggaaa agacgggcac ggatatggga aatatctcgc
    17641 aggagctgga aaaactgctc agctatacga tgggaagaga tgtgatcacg cgggcggata
    17701 tcgaggcagt ctgcacaacg cagattacga accggatttt cgagatgatc cgcgcggtaa
    17761 cggagaagaa gcagcggaaa gcgctcgatc tgtatcagga tctgctggct ttaaaggagc
    17821 cgccgatgcg gattctcttt ctgctggcac gccagttcaa tctgctgctg caggtcaaag
    17881 agctgctggc agagcactgc gatcaggcga cgatcgcaaa acggacggga ctgcagagct
    17941 tcgttgtgcg gaactatatt cccatgtcgc gccagtatgc ggcggaggaa ttaaaagcgg
    18001 cggtcaatga ctgcgtgcag atggaagagg acgtgaagac gggaaaaatt tccgatctgc
    18061 tgagcgtgga gctgctgatt acgaaataca gcagggcggc gtaagagcag agttttctat
    18121 agcaagaccc gaaaataatg caaaaaaggc agtgctg
//
LOCUS       DAOMUF010000022        17879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_596_length_17879_cov_4.267561, whole genome shotgun sequence.
ACCESSION   DAOMUF010000022 DAOMUF010000000
VERSION     DAOMUF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 17879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17879
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_596_length_17879_cov_4.267561"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..495
                     /locus_tag="PLN77_02660"
     CDS             64..495
                     /locus_tag="PLN77_02660"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRM43217.1"
                     /translation="MYQEEVKSAIILLYLGILAGMDVRKRQISLVLTGVLFLAGIGWQ
                     ILVEQTSWLEWLLSLLPGAGFLTLSYISREQVGYGDGLLLLTVGIWLGFGQTFGVLTL
                     GLILCSVLCGVLLMRKKIRKQDSIPLVPFLLLGFVGRCGML"
     gene            498..923
                     /locus_tag="PLN77_02665"
     CDS             498..923
                     /locus_tag="PLN77_02665"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HRM43218.1"
                     /translation="MKMRKASITVEAVLVVPLVLMVIFLLLSLTFFVHARSWYTFAAY
                     ESTMLAASEGRLSVEKGEAAAQSRMEWWISQIPLPAEPVTVQTVCREKEIQIKAEGNI
                     LPIWSRNAWEYSVLSESRRNNPVKTIRKIRAVKQIWNQK"
     gene            964..2433
                     /locus_tag="PLN77_02670"
     CDS             964..2433
                     /locus_tag="PLN77_02670"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HRM43219.1"
                     /translation="MNHTYVILQEEKEPDTASYQIRMLLTNAIPGLLDCKIGKMDDKT
                     LFYYEVTSRQSLQSVFEQRSIGAEALRKLFEQLLDLLEELGQYLLAPDGLVLQPELVF
                     ADANLENFSFCYLPGKQRHTGCFQKQIRELMEYLLPKLDHQEQDAVVLGYGLYREISA
                     EIFSVEMIQALLYQTGKEKKETTEQEGKERPREENPPVQMEETLWGEQEAEAAENFFV
                     EAEEEKDEGRWKNIVEAVICIGFLGGVLALRWTQAETVWYLVLGGAAAVVGIVSLALE
                     LVSRWKKRQSGTEIGSYGKAAGIRKQGETGDVFSEYQNRKGLKQKDGTWNPQSDTEQQ
                     GWRRDSSVEKEKTWEKNEEQENRKMHSLMELEGHEEDLFGKTELLTSPEDEGRAYLIP
                     IDPIDLPLIPIERGSTLIGKLSAAADVILPYRTVSRLHAKLVSTEEGDYLLDLNSRNG
                     TTVNGVPLMGETKKKLEDGDDISFAGKKYRYKAGEKNRN"
     gene            2741..3415
                     /locus_tag="PLN77_02675"
     CDS             2741..3415
                     /locus_tag="PLN77_02675"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRM43220.1"
                     /translation="MEEQRKNSYDPNEDIVDRMWKRSKNSINLVIVVCNILVFLAVTL
                     TGGTESSRHMMDCGAAYAPLIESGEYYRLFTSMFLHFGIQHLINNMLLLLFLGDYLER
                     AAGKIRYLVIYLGGGLVGNLCSYLHELSLMKTGEAPAVSAGASGAIFSAVGAMLVLLA
                     FRKGRLEDLTFRRMAVMAVLSLLVGFQSSNVDNFAHIGGFLAGAVLMAVLYPGMARIR
                     KRGKRP"
     gene            complement(3390..4073)
                     /locus_tag="PLN77_02680"
     CDS             complement(3390..4073)
                     /locus_tag="PLN77_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM43221.1"
                     /translation="MSKEPTTNSQGLSEECHAMISRISHEVRNPVAVIHSFHQLLLLA
                     HPELSNDLYFQKIQENMAFLNSLLDELSCYNHSYRAVRAYVNPYLLLQNLCADTGVLL
                     EKQQVSIELVKESAIPRFALDTTQFRQLFLNLIRNAAEAMPSGGTIKISLCFDGDFLT
                     IRVQDTGCGIPAEDLPTLFDLFVTHKKNGTGLGLAICKEIVTAHDGTISVSSVPGEGS
                     TFTVVFPFS"
     gene            4274..4693
                     /locus_tag="PLN77_02685"
     CDS             4274..4693
                     /locus_tag="PLN77_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRM43222.1"
                     /translation="MMDCIFCKLANGEIPTATLYEDDEFRVILDQGPATKGHALILPK
                     QHFANIYEIPDETAAKAMILGKKMATAMTKALHSDGFNLVQNNGEAAGQTVFHFHMHL
                     IPRYEGDNAGIAAWKQGTLEDAVKEQIIESVTAELKA"
     gene            4753..5277
                     /locus_tag="PLN77_02690"
     CDS             4753..5277
                     /locus_tag="PLN77_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM43223.1"
                     /translation="MDKNNFSEIYKKYVMTAYSAAMQVVHNEADADDIAQETMVKLYL
                     NIDALDEDRVEGWVRVAAKNLGIDYQRKAQRFDWNELDEDLRAEEMELPTLAEEEKQE
                     RNHFFREVMDALKDHDESGYYITREIKVHERSEKEVASEMGITIGNLRVKMTRTKKWL
                     CQHFADKARERGFL"
     gene            complement(5281..6348)
                     /locus_tag="PLN77_02695"
     CDS             complement(5281..6348)
                     /locus_tag="PLN77_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRM43224.1"
                     /translation="MKHIVLTGGGTAGHVTPNIALIPKLKEAGYKISYIGSYDGMERK
                     LIEDLGIPYYGISSGKLRRYFDPKNFSDPFRVVKGYFEAKKLLKKLKPNVVFSKGGFV
                     SVPVVLAAKACKVPALIHESDMTPGLANKLCIPSAAKVCCNFPETVKCLPENKAVLTG
                     TPIRQELLSGDAREGLKFCGFNAAKPVILVIGGSLGSVAVNNAVRSILPELLKDFQVI
                     HLCGKDKLDASLNGTEGYVQFEYIKDELPDLFAAADLIISRAGANAICEISALAKPNI
                     LIPLSANASRGDQILNARSFERQGYSKVLEEESVNAQTLQSAIREVYENRQTYINAMK
                     KSSHTDSIGRILSLIQECERK"
     gene            6432..7385
                     /locus_tag="PLN77_02700"
     CDS             6432..7385
                     /locus_tag="PLN77_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HRM43225.1"
                     /translation="MENAEERKKMKKIEKEEQLKKKLEQTMEEKMAADSKTVEGESAV
                     VKAEEGNTATEQEAAPALSEEELNAMYERFQQRLQKEGLADAEGNPTEKTLEIRRQIV
                     EKKVEREKQAERQKKIAKRAKTTKHWVQTAKAAGVVLVVGACVFGASMTSEANRIRLV
                     ETISGVRNSGDTTWVDNGEDRKYTAEDWDKVKEEIRDKLHIAVPEFYYIPEGMEYSGS
                     SILQEIQVAIIKYRYNEHIVYFQLSANEKDLSQGNWKDREKVEVETLDDTVEVEMGTI
                     SENGEENYYVIWKYKDGYYELSGQIEKEELIKILNEMQYNM"
     gene            7443..7940
                     /locus_tag="PLN77_02705"
     CDS             7443..7940
                     /locus_tag="PLN77_02705"
                     /inference="COORDINATES: protein motif:HMM:NF040335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6147 family protein"
                     /protein_id="HRM43226.1"
                     /translation="MKKVMNLKSLLFCAMLVLTLAVNTVSVQADALDYLGNTIDGSVL
                     TNETESIGNYQSVARSTYLHQGFVRITNNGNGYIGIGGGTECNVTCGTVKLNIYLERS
                     TGSGSFYSYKKWENVDYNTDSLFMSKEIKVEKGYYYRLRGYHSCTKSGVTENGGSSTN
                     GIYIG"
     gene            8604..10226
                     /locus_tag="PLN77_02710"
     CDS             8604..10226
                     /locus_tag="PLN77_02710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM43227.1"
                     /translation="MRLTFLGATHEVTGSCYYLEAAGKKFLVDCGMEQGPNIYENKPI
                     PVPASQLDFVLITHAHIDHTGNLPLLYAHGFRGPIYMTPATVALSDIMLRDSAHIQIA
                     EAEWRNRKNRRAGKEDYVTPYTMEDALGVIRLLEGIPYHQRVTLSDGIQIRFLDAGHL
                     LGSASIELWLTEDGVTKKLLFSGDIGNIHQPLINDPEYPESADYVIMESTYGDRSHGP
                     KPDYVPELAKIIQETLDRGGNLVIPSFAVGRTQEMLYFIREIKAEHLVHGHGEFPVYV
                     DSPLAVEATNIFRDHQKECYDSDAAALLAQGINPILFPGLKLSITSDESKAINFNETP
                     KVIISASGMCDAGRIKHHLKHNLWRQESTVLFVGYQAVGTLGRALIGGAKEVKLFQEE
                     VHVNAHIIQFPGMSGHADQEGLLKWAGSLQAKPDRVFVTHGEDKVTEIFAEKLWDEFR
                     YPAMAPFSGTVFDLKENEFIEKTVGIPIQKETTANAGTLRTRSSGNAVFDRLVAAGQR
                     LLTVITRNKGGANKDLSKFADQINSLCDKWDR"
     gene            complement(10411..10653)
                     /locus_tag="PLN77_02715"
     CDS             complement(10411..10653)
                     /locus_tag="PLN77_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HRM43228.1"
                     /translation="MEYTTKGTCSRKILFEVEDGKVHNVQFVGGCNGNTQGVARLVEG
                     MDVNEAISRMKGIDCNGRGTSCPDQLAKALEQAIQK"
     gene            10958..11656
                     /locus_tag="PLN77_02720"
     CDS             10958..11656
                     /locus_tag="PLN77_02720"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRM43229.1"
                     /translation="MKLIGIIGAMEVEVASLKEMMTDVTIRTKASMEFNHGYLNGKEV
                     VVVRSGIGKVNAGICTQILVDDYGVDCVINTGIAGSLRNEINIGDIVISTDAVQHDMD
                     VRIFGYPLGEIPQMGVLSFPADEHLAEVAEEVCKEVNPEIQVFRGRVVSGDQFISSKE
                     VKNHLIEEFNGSCAEMEGAAIAQGAYLNKIPYVVLRAISDKADDSATMDYPTFESEAA
                     RHCVNLLQEMVKRL"
     gene            12152..12937
                     /locus_tag="PLN77_02725"
     CDS             12152..12937
                     /locus_tag="PLN77_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43230.1"
                     /translation="MLEFCVKQNVFLYAMIAAGIAGVWCLFWNNRFYTRAIRDLGRLK
                     EPKSKWTRSVLETCQRKAHPFSNAGAFMRSQIAQGRSAGMAVPTLLRGSQNMILTVLA
                     LSILSLWAIYRYRYGEAELMRYLGTGIALAGGLVFFRLCLDFGGKEEILLNGWTDYLE
                     NEFAATKQSAVLETVQQTERQPVQAETMAQAGTGAAKAKPQTAGSESAKERNELFADP
                     EKEARISKVREGIRQTAAAESRFAGMLTPEEEKLMREIISEYV"
     gene            13113..14069
                     /locus_tag="PLN77_02730"
     CDS             13113..14069
                     /locus_tag="PLN77_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRM43231.1"
                     /translation="MRLVILEAESLGKDMDLSGFSRFGEVTVYEKTTEEECPERVREA
                     DIVICNKVRMCSRTLSGAENLKLICVTATGINNIDLDYVKRHNIAVTNVAGYSTVSVA
                     QHTFAMYFYLAEKLRYYDDYVKNGSYAASSLFTCMDRPFTELEGKTWGIIGLGAIGRK
                     VASIAQAFGCRVIYYSTSGAHDDPDYERVDLYRLLKDSDVVSIHAPLNEATENLMNIE
                     TFANMKRNAILINVGRGSIVNERDLCCALREGLIAGAALDVLSSEPIRSDSPLLKMAD
                     DDRLYITPHIAWASKEARTRLIGLVQKNIASYLEGGMENRVV"
     gene            complement(14217..14480)
                     /locus_tag="PLN77_02735"
     CDS             complement(14217..14480)
                     /locus_tag="PLN77_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43232.1"
                     /translation="MFQMWGKLVENNHILQDLTVCIDDEDTRTHKVFRALDEICDAWN
                     LAKPIWLDTNISEFKRRSKTRFTQDNFIESLEFDYLEIQITEE"
     gene            complement(14535..15410)
                     /locus_tag="PLN77_02740"
     CDS             complement(14535..15410)
                     /locus_tag="PLN77_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM43233.1"
                     /translation="MKEKLQTKFATRQYMISDTFELYYYSTDDVPGVKEHTHTHYEFY
                     LFLEGAISLEIDGVLYTPTPGDLLLIPPGVPHHLVMHDLSVPYRRFVFWVSAAFAEQL
                     RARSADYVYLMEYAAENQKYLFPLDSVEFNTIQYRTIRLIEEMRSDHFGKETQIFLYV
                     SDLLMQINRTVYEQHHPLTRKAQLSLYEQLCFYIDEHLSEDLSLERLAAELFVSKYHI
                     AHVFKDEIGMSIHQYISKKRLARCREALLGDVPVTKVYLLFGFHDYSSFYRAFKKEYG
                     ISPKDFKELHTVTSD"
     gene            15615..16253
                     /locus_tag="PLN77_02745"
     CDS             15615..16253
                     /locus_tag="PLN77_02745"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HRM43234.1"
                     /translation="MNQILEQLGTYGIVPVVVLQDAAKAEPLAEALCKGGLACAEVTF
                     RTDAAEESIRIMSEKFPEMLVGAGTVLTTEQADRAVKAGAKFIVSPGLNPEVVKWCQA
                     HEVPVIPGIVTPTEMAQAIGLGLTMVKFFPAEPAGGLKYIRAIAAPYTMMKFMPTGGI
                     NPQNVREYLAYDRIAACGGSWMVKNTMIENNEFDRIEELVKEAVEIVKESRT"
     gene            16284..16691
                     /locus_tag="PLN77_02750"
                     /pseudo
     CDS             16284..16691
                     /locus_tag="PLN77_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
     gene            16773..17030
                     /locus_tag="PLN77_02755"
     CDS             16773..17030
                     /locus_tag="PLN77_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43235.1"
                     /translation="MNELYEMIEKKIKESGYPRPISGSDVYDDICDQIEGKENGSYVV
                     LSKFDDDVIFEYHITVMDDEFNLGVLTMRTPEGVFETDFDQ"
     gene            17057..17662
                     /locus_tag="PLN77_02760"
     CDS             17057..17662
                     /locus_tag="PLN77_02760"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM43236.1"
                     /translation="MPNVEKITKQTENPFVNLYLVEGTNKVNHHFRYYVASRAKSIEQ
                     MKIKTRKNTPDGVLMYSLYGEKRDKIVLVRQYRVSIDDYIYEFPAGLVEPGEEFHEAA
                     VREMHEETGLTFHPLKVDPCYEKPFFTTIGLTDEACSAVYGWCEGEISRKYLEESEVI
                     EVVLADREEAKRILKEENVAIMCSYHLMHFIHDEEPFAFLK"
BASE COUNT         5237 a         3772 c         4869 g         4001 t
ORIGIN      
        1 gggtgagacc ggaaagaagg acggtaaaca gaaactcggg tttgcggaaa ggaggccgcg
       61 gctgtgtatc aggaagaagt aaagagtgca attatattgc tgtatctggg gattcttgcc
      121 ggaatggatg tgaggaaacg gcagatcagc cttgtcctga ccggtgtttt attccttgca
      181 ggcattggat ggcagattct ggtggaacag acatcgtggc tggaatggct gctgtccctg
      241 ctgccgggag cgggatttct gacgctcagt tatatatcac gggaacaggt gggatacggg
      301 gacggccttc tgcttctgac ggttggaatc tggctgggat ttgggcagac attcggcgtt
      361 ctgacgctgg gactcattct ctgttcggta ctctgcggag tccttctgat gcggaagaaa
      421 atacggaaac aggattccat accgctggtg ccgtttctgc tgcttggatt tgtggggagg
      481 tgcggaatgc tttgaaagtg aaaatgagaa aagcaagtat tacggtagag gcagttctgg
      541 tcgtgccgct tgttctgatg gtgatttttc ttctgctgtc gctgacattt tttgtgcacg
      601 cacggagctg gtatacattt gcggcatatg aaagcacaat gcttgccgcc agtgaaggcc
      661 gcctgtctgt ggaaaaagga gaggcagccg cccaaagccg gatggaatgg tggatttccc
      721 agataccgct tccggctgaa ccggtgaccg tacagacggt gtgcagagaa aaagagattc
      781 agataaaagc agaaggaaac attctgccaa tctggagccg gaacgcgtgg gaatattcgg
      841 ttttgagtga aagcaggaga aacaatccgg tgaaaacgat ccggaagata cgggcagtaa
      901 aacagatctg gaaccagaag taaggacgga agcggagaaa tgcagattga ttataaaaga
      961 gatttgaatc atacatatgt tattttacag gaggaaaaag agccggacac ggcgtcgtat
     1021 cagatccgta tgctgctgac aaacgcaatc ccagggcttt tggactgcaa aatcgggaaa
     1081 atggatgata aaacattgtt ttactatgag gtgacatcaa ggcagtcact tcagagtgtg
     1141 tttgaacagc gctcgattgg ggcggaggcg ctgcggaaac tgtttgaaca gctgctggat
     1201 cttctcgaag aactggggca gtacctgctt gcaccggacg gcctcgttct gcagccggag
     1261 ctggtttttg cagatgcaaa tctggagaac ttttccttct gttatctgcc gggaaagcag
     1321 agacacacag gatgttttca gaagcagatc cgggagctga tggaatatct gctgccgaag
     1381 ctggatcacc aggagcagga tgcggtggta ctcgggtatg gactgtatcg ggagatttcg
     1441 gcggagatct tttcggttga gatgattcag gcactcttat atcagacagg gaaagagaag
     1501 aaagaaacga cggagcagga gggaaaagag cgtcctaggg aagaaaatcc gcctgtacag
     1561 atggaggaaa cactttgggg agaacaagaa gcggaagcgg cggaaaattt ttttgtggaa
     1621 gcggaggaag aaaaggatga gggacgctgg aaaaatattg tggaagcagt gatctgcatc
     1681 ggatttctgg gaggtgtgct ggcactgcgg tggacacagg ccgaaactgt ttggtatctg
     1741 gtgctgggag gagcggcggc tgttgtggga attgtaagtc tggcattgga acttgtcagt
     1801 cgatggaaaa agcggcagtc gggaacagaa atcggaagct atgggaaggc cgcaggaatc
     1861 cggaaacaag gagaaacagg agatgtattt tcggaatatc agaatcggaa gggcttaaaa
     1921 cagaaagatg gaacatggaa tccgcaaagc gatacagaac agcagggatg gagaagggat
     1981 tcctcagtcg aaaaagagaa aacatgggaa aagaatgaag aacaggaaaa cagaaagatg
     2041 cacagcctga tggaattgga ggggcatgaa gaggatctgt ttggaaaaac ggaactgctg
     2101 accagcccgg aggatgaggg aagggcatat ctgattccga tagatccgat tgatcttccg
     2161 ctgattccga ttgaacgcgg aagcacattg attggaaaac tgtcagcggc ggcagatgta
     2221 attctgccat accgtacggt aagccgcctt cacgcaaagc tggtcagtac agaagaggga
     2281 gattatctgc tggatctgaa ttcgcggaac ggaacaacgg tgaatggcgt tccattgatg
     2341 ggcgagacaa aaaagaagct ggaggatgga gacgatatct catttgccgg gaagaaatac
     2401 cggtataagg cgggagagaa aaacagaaat tagagaaaaa tgacgcatta cactttttcg
     2461 atttggagaa agtgctggat gcgttatttt taatatggca gaatgatcga agaaaaaagt
     2521 tatgcgaatg aaacaaaaat attatagaaa agaaaattat gcataaaatt aaaaaaagaa
     2581 ataatgtaaa aaataatgtc ataaaacgtc gaaagccgtc gtgaagcgtc aataattgtc
     2641 gaaaaacgtt tatcaaaaaa gaaaaaaatt tgacgatttc ttgcatttac aagtgaatta
     2701 aattataata aaaaaatagc acagataagg aaatgatgta atggaagaac aaaggaaaaa
     2761 cagttacgat ccgaacgaag atatcgtgga ccgtatgtgg aagagatcaa aaaacagcat
     2821 caatctggtc atcgttgtat gcaatattct ggtattcctt gcagtcacac tgaccggagg
     2881 gacagagagc tccagacata tgatggactg cggtgcggcg tatgcgccgc tgattgagag
     2941 cggagaatac tatcgcctgt ttacgagtat gtttcttcac tttggcatcc agcatctgat
     3001 caacaacatg ctgcttcttt tgttccttgg ggattacctg gaacgcgcag ctggaaagat
     3061 ccgttatctg gtcatctatc tgggcggcgg actcgtcgga aatctgtgct cgtatctgca
     3121 tgaactgtcc ctgatgaaaa caggggaagc accggcagtt tccgccggcg cttcgggagc
     3181 gattttttct gcagtcggag cgatgcttgt tcttctggca tttcggaaag gaaggctgga
     3241 ggatctcacc ttccggcgga tggccgtcat ggctgtcctt tcgctgctgg tcggattcca
     3301 gtcttcaaat gtggacaact tcgcacacat cggcggtttt ctggcaggtg cagtcctgat
     3361 ggcggttctc tatccgggaa tggcaaggat caggaaaagg ggaaaacgac cgtaaaggtg
     3421 cttccttccc cgggaacgga agaaacggaa atcgtgccgt cgtgtgcggt tacaatttcc
     3481 ttgcagatgg caagtccaag gccggttccg tttttcttat gtgtgacaaa aaggtcaaag
     3541 agagtcggca gatcctcggc ggggattccg cagccagtgt cctgcacacg gatggtcaga
     3601 aagtcaccgt cgaaacagag agagattttg atcgttccgc cggaaggcat tgcttccgcc
     3661 gcatttcgga tcaggttcag aaaaagctga cgaaattgcg tggtatcaag ggcaaagcgc
     3721 ggaatggcag attccttgac aagttcaatg gaaacctgtt gtttttcgag gagaacgccg
     3781 gtatcggcac agagattctg gagcagaaga taaggattca cgtaagcgcg taccgcccga
     3841 tatgaatgat tatagcagga gagctcatcg agcagggagt tcagaaaagc catattttcc
     3901 tggattttct gaaaatagag atcgttggaa agctccggat gcgctaaaag aagaagctga
     3961 tgaaagctgt ggataaccgc aacaggattt cgaacctcat gtgaaatccg ggaaatcata
     4021 gcgtgacatt cttccgataa gccctgtgaa ttggttgttg gttcttttga catagccgta
     4081 tcctccttat gatgcgtagg taatgatttt tgattaggca aaagtttagc attgaaaagg
     4141 gggagcgcgc aacaaaaagc gttctgcatt atttgacatt tttcgacaaa tagtgagaaa
     4201 atagagttgt tcggaaccgt gcagaacggg attcggaaca acagaagaat taaaaaaagt
     4261 aaaggagaaa aagatcatgg attgtatttt ttgtaaatta gcaaacggag aaattccaac
     4321 cgcgacattg tatgaggacg atgagttccg tgtcattctg gatcagggac cggcaacgaa
     4381 aggacatgcg ctgattcttc caaaacagca ttttgcaaac atttatgaaa ttccggatga
     4441 aaccgcagcg aaagcaatga ttctcggcaa aaaaatggcg acggccatga cgaaagcgct
     4501 tcattccgat ggcttcaatc tggtacagaa caatggtgag gcagcaggac agacggtatt
     4561 ccacttccat atgcatctga ttccaagata tgaaggtgac aatgccggta ttgctgcatg
     4621 gaagcaggga actctcgagg atgcggtaaa agagcagatc attgagagcg tgacggcaga
     4681 gctgaaggcc tgagaaaagg ctggcagcag ccagaaaaag aatcaatgac agacgaacag
     4741 gggcggagaa caatggacaa aaataacttt tcagaaatat ataaaaagta tgtaatgaca
     4801 gcctattcag ccgcaatgca ggtggtacac aatgaagccg atgcggatga tatcgcacag
     4861 gaaacgatgg tgaaactgta cctgaatatt gatgcgctgg atgaagacag agtagaaggc
     4921 tgggtccgtg tcgctgcaaa gaatctggga attgattatc aaagaaaagc acaacgtttt
     4981 gactggaacg aactggatga ggatcttcgg gcggaagaga tggaacttcc gacgcttgca
     5041 gaggaagaaa agcaggagag aaatcatttt tttcgggaag tgatggatgc actgaaagat
     5101 catgatgaat cgggttatta tattacgcgt gaaatcaagg tgcatgagcg ttcggaaaag
     5161 gaagtagcat ccgaaatggg tatcaccatc ggaaatctgc gggtgaaaat gacccggacg
     5221 aaaaaatggc tttgccagca ctttgctgac aaagccagag aaaggggatt tctgtagtta
     5281 ctatttgcgt tcgcattcct ggatcaggga aaggatgcgg ccgatggaat cggtgtggct
     5341 gctcttcttc atcgcattga tgtaggtctg acggttttcg taaacctcac ggattgcgga
     5401 ctgaagagtc tgcgcattca cggattcttc ttccagaact ttactgtaac cctgacgctc
     5461 aaaggaacgc gcatttaaaa tctggtctcc gcggctggca ttggcagaaa gcggaatcag
     5521 aatgtttggt tttgccagag cactgatttc gcagatcgca tttgcgccgg cgcgggagat
     5581 gatcagatct gctgcggcga agagatccgg gagctcatct ttgatgtact cgaactgaac
     5641 gtaaccttcg gtaccattta aggatgcatc cagtttatct ttaccgcaga gatgaatcac
     5701 ctggaaatct ttcagaagtt ctggtaaaat agaacggacc gcattattga cagcgacgga
     5761 accgaggctt cctccgatga cgagaataac cggttttgcg gcattgaatc cgcagaactt
     5821 caggccttcc cgcgcatcgc cggacagcag ttcctggcgg attggcgttc cggtcagaac
     5881 ggctttgttt tccgggagac atttgacggt ttccgggaaa ttacagcaga cctttgcagc
     5941 ggacggaata cacagtttat ttgccaggcc aggcgtcata tctgattcat ggatgagcgc
     6001 cgggacttta catgctttgg cggcaaggac aaccggtacc gaaacgaagc cgcctttgga
     6061 gaagacaacg tttggtttca gctttttgag cagttttttt gcttcaaagt agccttttac
     6121 gacgcggaat ggatcggaaa agtttttcgg atcgaagtag cggcgcagct ttccggatga
     6181 aattccgtaa tacggaatgc cgagatcttc gatcagcttg cgctccatac cgtcgtagga
     6241 accgatataa gagattttat aacctgcttc ctttaatttt ggaatcaggg caatattcgg
     6301 ggtaacatgg ccggcggtac ctccgcctgt cagaacaata tgcttcatga gaattcctcc
     6361 ctggaatttg ataatatcat ttggctttta ttatcccctc aagaagaaaa aaagtcaaga
     6421 gcggttacaa catggaaaat gccgaggaac ggaagaagat gaaaaagata gaaaaagaag
     6481 aacagctgaa aaagaaactg gagcagacga tggaagaaaa aatggctgcg gattcgaaaa
     6541 cggtggaagg ggaatcggca gttgtaaagg cagaggaagg aaacacagca acagaacagg
     6601 aggctgcgcc tgcgctttca gaggaagaac tgaacgcaat gtacgagcgc ttccagcagc
     6661 gccttcagaa ggaaggactc gcggacgcgg aaggaaatcc gacagaaaaa acactggaga
     6721 tccgccgcca gatcgtggag aaaaaggtgg aacgcgagaa gcaggcggag agacagaaga
     6781 agattgcgaa gagagcgaaa acaacaaaac actgggtaca gacggcaaag gcggctggtg
     6841 ttgtgttggt tgtgggagct tgcgtgtttg gagccagtat gacgagcgag gcgaatcgga
     6901 ttcggttggt ggagacgatt agtggggttc ggaattcagg agatactaca tgggttgata
     6961 atggagaaga cagaaaatat acagcagaag attgggataa ggtaaaagaa gaaataaggg
     7021 ataagttgca tatagcggta cctgaatttt attatattcc ggaaggaatg gaatatagcg
     7081 ggagttctat attacaagaa atacaggtgg ctattatcaa gtacagatat aatgaacaca
     7141 tagtgtattt tcaattatca gcaaacgaga aagatttatc gcaaggtaat tggaaagata
     7201 gggaaaaagt tgaagtagag acgcttgatg acactgtaga ggttgaaatg ggaacaatat
     7261 ctgaaaatgg tgaagaaaat tattatgtta tatggaaata taaagatgga tattatgagt
     7321 taagtggtca aatagaaaaa gaggaattga taaaaatttt gaatgaaatg cagtataata
     7381 tgtaattgat ttaaaattaa tacgtatata tcatgtagat gagacgaggg gaaagaggaa
     7441 gtatgaaaaa ggttatgaat ttaaaaagct tacttttttg tgcgatgctc gttttgacgc
     7501 tggctgtaaa tacggtgagt gtacaggcag atgcattaga ttatttaggt aatacaattg
     7561 atggatctgt attgacaaat gaaacagaat caattgggaa ttaccaaagc gtggcaagaa
     7621 gtacatatct tcatcagggg tttgtgagaa ttacaaataa tggtaatgga tatattggaa
     7681 taggtggcgg aacagagtgt aatgttactt gtggaactgt aaaattaaat atttatttgg
     7741 aacgttcgac aggatcaggc agtttttata gttacaaaaa gtgggaaaat gtagactata
     7801 atacggattc tttatttatg agtaaggaaa taaaagtaga aaaaggttat tattaccgtt
     7861 taagaggtta tcattcctgt actaagagtg gtgttactga gaatggtgga agttcaacca
     7921 atggaatcta tattggataa taatttttca cctaacatgt agatatcaat attgaaaaca
     7981 agcaaaagta taattcatac aagcacaaga gagtagagta aatgaacgga aacaattttt
     8041 agactagtag tttgcaactc cctcacccct taaaaatccc ggggccagtc ccctatagaa
     8101 accttataac aatgaatata ttaagacgaa aacgcagatc tttcaggatt ttcaacgcgt
     8161 cgaaattcct ttttccaacc acaacacaga tctgagtcat catgtgttac tatgttcagc
     8221 gtgtgagtga tcatacggaa catcggctgg ccccgataag tgaggcatcg gtattactgc
     8281 aggaataccg gtgcctcttt tctatgcact tccggcggcc gatgcccata attgaacaaa
     8341 aatatcaaga ttgttgtgca atctgcctta aaattccgtt ggaatctgcc agatattatg
     8401 ctataataat caaaaaagag agaataagca taaaatacaa aaggaatttc agattatttt
     8461 cgcggacaac gcgtcaaaga ccatgctggc atgggaattt gacgactgct ggccgcgata
     8521 acgggccaaa cgtataacgc gtgttttgac ccgcagtgac aacattttgt ccccgaacaa
     8581 aacgggagaa caggaggcat cttatgcgtc ttacatttct tggagcaaca catgaagtga
     8641 cgggcagttg ttattatctg gaggctgccg gaaagaagtt tttagtggac tgcggtatgg
     8701 agcagggacc gaatatttat gaaaacaagc cgattccggt tccggcatcc cagctggatt
     8761 ttgtattgat cacgcacgcg cacatcgatc acaccggaaa tctgccgctg ctgtatgcgc
     8821 acggcttccg cggcccaatc tatatgactc cggcgacggt agcgctttcg gacatcatgc
     8881 tccgcgacag tgcacacatt cagatcgccg aggccgagtg gcggaaccgc aagaaccgcc
     8941 gggcgggaaa agaagattac gtcacaccat acacaatgga ggacgccctc ggcgtgatcc
     9001 gcttgctgga gggcattccg tatcatcagc gtgtgacact atcggacggc attcagatcc
     9061 gtttcctcga tgccggtcat ctgctcggct ccgcatccat tgagctgtgg ctgacagagg
     9121 acggtgtgac gaaaaaactg ctcttctccg gcgatatcgg aaatatccat cagccgctga
     9181 tcaacgaccc ggagtaccca gagagcgctg attacgtgat tatggaatcc acctacggtg
     9241 accggagcca cggtccgaaa ccggattatg taccggagct ggcaaaaatc attcaggaaa
     9301 cgctcgaccg cggcggcaac ctcgtgatcc cgtcgtttgc tgttggaaga acgcaggaga
     9361 tgctgtactt tattcgtgaa attaaggcag aacatctggt tcacggccac ggcgaattcc
     9421 cggtttacgt ggacagtccg cttgccgttg aggcaaccaa tattttccgc gatcatcaga
     9481 aagagtgcta cgattccgat gcagctgcgc tccttgcgca gggcattaat ccgattctgt
     9541 ttccgggctt aaagctctcg attacatccg atgaatcgaa agccatcaac ttcaacgaaa
     9601 ccccgaaggt catcatatcc gcgagcggca tgtgcgatgc aggacgaatc aagcatcatc
     9661 tgaagcacaa cctctggcgg caggagagta cggttctctt cgtcggctat caggccgtcg
     9721 gtacactggg acgggcgctg atcggaggcg cgaaggaagt gaaactgttc caggaagagg
     9781 ttcatgtgaa cgcgcacatc atccagttcc cgggcatgag cggccacgca gatcaggaag
     9841 gactgctcaa atgggcgggc agtctgcagg caaaaccgga ccgcgttttc gtaacgcacg
     9901 gcgaagataa agtgacggaa atttttgcag aaaaactgtg ggacgaattc cgctacccgg
     9961 cgatggctcc gttctccggc acggtatttg acctgaaaga aaatgaattc atcgaaaaaa
    10021 ccgttggcat tccgatccag aaggaaacaa cggcaaacgc aggaacactg cgcacgagaa
    10081 gcagcggaaa tgccgtcttc gatcgtctcg ttgcggctgg tcagcggctg ctcaccgtca
    10141 ttacaagaaa caaaggcggt gctaacaaag acctttcgaa atttgccgat cagatcaaca
    10201 gtctctgcga caaatgggac agataaagca gtccggtggc tggaagaaag actgtgctat
    10261 taatatagaa gaaaaaccgg aagaaagcag gcggcaggga agctgtgctg catcagaaaa
    10321 gaaaaaacag aagtggataa attacaaact ccccggggtg agcattcccc ggggagttat
    10381 tataataaaa aagagcaagc gtaaaaaaag ttatttctgg atagcctgtt ccagtgcttt
    10441 tgcaagctgg tctggacatg atgttccgcg tccgttgcag tcgatgcctt tcatgcggga
    10501 gatggcttcg tttacgtcca tgccctcaac gagtcttgca acaccctgtg tgtttccgtt
    10561 gcatccgcct acgaactgta cgttgtgtac tttaccgtct tctacctcaa acagaatttt
    10621 tctggaacag gtaccttttg ttgtatattc catcgataag atcctccttt gtgaataaaa
    10681 aattagaaca ttatcgtgaa cttcagccac gataattctt cggttcctgc tattatacca
    10741 tagattacga aaacagcaaa gaaaaaagat accacagatc caaaatttat tgtatactgt
    10801 aacaggaatt acaaagaaac cagaaggaaa agaaaatgga ctcccggagt tttccgttct
    10861 tactttagcg gctgaccgct gacaaaaatg atttcataaa agcaagaaca gaaagaatcg
    10921 gagagtacat gtaaagttat tggaggatat cataaacatg aagttaatcg gaattatcgg
    10981 tgcaatggaa gttgaagttg caagcttaaa ggaaatgatg acggatgtta caatccgcac
    11041 gaaggcatcc atggagttca accacggtta cctgaacgga aaagaagtgg ttgttgtgcg
    11101 cagcggaatc ggtaaagtaa atgccggtat ctgcacgcag attcttgtgg atgattacgg
    11161 cgtggactgt gtcatcaaca ccggaatcgc aggctccctg cggaatgaaa tcaacatcgg
    11221 cgatatcgtc atctcgaccg atgctgttca gcacgacatg gatgtgcgca tcttcggata
    11281 cccgctcggc gagattccgc agatgggcgt tttaagcttc ccggcagacg agcaccttgc
    11341 agaggtggca gaggaagtct gcaaggaagt caatccggaa atccaggtat tccgcggacg
    11401 cgtggtcagc ggtgaccagt ttatttccag caaagaagtg aaaaaccatc tgatcgagga
    11461 attcaacggt tcctgcgccg agatggaggg cgctgcgatt gcgcagggcg cttatctgaa
    11521 caagattcca tacgtcgtac tccgcgccat ctccgacaaa gcagacgaca gcgcaacaat
    11581 ggactacccg acctttgaga gcgaggcagc ccgccactgt gtcaacctgc tgcaggaaat
    11641 ggtaaaacgc ctgtaatcta aaaaaagcaa gaagaaaaaa tcgacaaacg ggcaggcgaa
    11701 aaccagcgaa accacgcgaa ccggcacgaa agcagtcccc tgaaggaacg aaaaataaaa
    11761 attcacgcga aaaagcacag aatcacaaag aatttgacga acgttttttg tttccaaatt
    11821 tttccggcag tgctatactg tggcacatcg gaacaagaag attctcacta cggattgctc
    11881 agcagtaaaa cacttgctgt tttgctgcgt aagaaagtga ttcaggtgtg agcagccctc
    11941 cttttgcgaa gcaaaatttt caatgagggc tgcgaatgtt acagttcaca ggtacctggg
    12001 aactgtaaca cgtctccact gccgatatgc cagcggaaga atcctgtgcg cgcaaattct
    12061 gccgcggcat ccgccgatgc accgcaacgg aacgggagca ccggcatatg atcatgacag
    12121 gcaaattcaa aaatcatcag ggggaataag aatgctggag ttttgtgtga aacaaaatgt
    12181 gttcttatac gcaatgatag cagcaggaat cgccggtgtg tggtgcctgt tctggaataa
    12241 ccgtttctac acccgggcca tccgcgatct cggaaggctg aaggaaccaa agtcgaaatg
    12301 gaccaggtcg gtgctggaaa cgtgtcagag aaaagcgcat cccttttcga atgccggggc
    12361 gtttatgcgc agccagattg cacagggcag gtcagcggga atggcagtcc cgacgcttct
    12421 gcgcggaagc cagaatatga tcctcacggt tctggcgctg tcgattcttt cgctgtgggc
    12481 gatctaccga taccgttatg gagaggcgga gctgatgcgc tacctcggaa cgggcatcgc
    12541 gctcgccgga ggccttgtct ttttccggct ctgtctggat ttcggcggaa aagaggaaat
    12601 tctgctgaac ggctggacag actatctgga aaatgagttt gcagcgacga aacagagtgc
    12661 ggtgctggaa accgtacagc agaccgaacg gcagccggtg caggcggaaa ccatggcaca
    12721 ggcaggaacc ggagcggcga aagcaaaacc gcagactgcg ggaagcgagt ccgccaaaga
    12781 gcggaatgaa ctgttcgcag acccggaaaa agaagcgagg atttcgaaag tcagggaggg
    12841 catccgccag accgccgcgg ccgagagcag gtttgccgga atgctgacgc ccgaggaaga
    12901 gaagctgatg agggagatta tcagcgaata cgtgtaaata ccaggggatc aggccgggga
    12961 gcatggcata tcgacatgac catatttccg aatcggcagt ccgcaggtat ctaagataaa
    13021 aatatttcag gcagtgccgt ctttcaaaac agacaggcgg cactgctttc ccggataaag
    13081 cagaatcatc atctgcaaaa ggaggaacca acatgagact tgtaattctt gaggcagagt
    13141 cactgggaaa ggacatggat ctgtccggct tttcccgttt tggagaagtg acagtatatg
    13201 aaaaaacaac ggaggaggaa tgcccggagc gtgtgcggga ggcggatatt gtcatctgca
    13261 acaaggtgcg catgtgcagc cgcaccctgt ccggcgcgga gaacctgaaa ctgatctgcg
    13321 tcaccgcaac cggcatcaac aacatcgatc tcgattatgt gaagcgccac aacatcgccg
    13381 tgacgaacgt cgccggctat tccaccgtca gcgtggccca gcacaccttt gcgatgtact
    13441 tctatctggc agaaaaactc cgttattacg atgattacgt aaaaaacggc agctacgccg
    13501 ccagcagtct tttcacctgc atggacagac cgttcaccga gctggagggc aagacgtggg
    13561 gcatcatcgg ccttggcgcg atcggcagaa aagtggcttc gattgcacag gcattcggct
    13621 gccgtgtgat ctattactcc acctccggtg cacacgacga tccggactac gagcgggtcg
    13681 atctctatcg tctgctgaag gattctgatg tggtttccat ccacgccccg ttaaatgagg
    13741 cgaccgagaa cctgatgaat atcgaaacgt ttgccaacat gaagcggaac gcaattctga
    13801 tcaacgtcgg ccgcggctcg atcgtgaatg agcgtgacct ctgctgtgcc ctgcgcgagg
    13861 gcctgatcgc cggagcagcg ctcgatgtac tttccagcga gccgatccgc agtgacagtc
    13921 cgttgctgaa aatggcggac gacgaccgcc tgtacatcac gccgcacatc gcctgggcat
    13981 cgaaggaagc ccgcacgcgc ctgatcggac tggtgcagaa aaatattgct tcgtacctgg
    14041 aaggcggcat ggaaaaccgc gtggtataaa agggatacaa atccagggac aaaatacata
    14101 aaaagagccg gacatccgac ggaaccgcag aggggactgt aagaacagcc gctcccaagt
    14161 tccgccggat gcccggcttt tgcatttgct ttattcgctg caaaaacgag agaacattat
    14221 tcctctgtaa tctgaatttc cagataatca aactccagcg attcaataaa attatcctgt
    14281 gtgaaccgcg ttttcgatct ccgtttaaac tccgaaatat tcgtatcgag ccagatcggc
    14341 ttcgccagat tccaggcatc acagatttca tcgagcgcac ggaagacctt gtgcgttctg
    14401 gtatcctcat cgtcgatgca gacggtaaga tcctgaagaa tatgattgtt ttccactaat
    14461 tttccccaca tctgaaacat attaaaaatc ctcctgaaaa gtacaaagtg aacagtgcat
    14521 ttggaacgac cgtgttaatc gctggtaacg gtatgcagtt ctttaaaatc cttcggcgag
    14581 atcccgtact cttttttaaa cgcgcggtaa aaactcgaat agtcgtgaaa tccgaacagc
    14641 agatacacct tcgtgaccgg tacatcgccg agcagcgctt cacggcacct tgccagccgc
    14701 tttttggaaa tgtactggtg gatcgacatg ccgatttcat ccttgaaaac gtgtgcaatg
    14761 tgatatttgc tcacaaaaag ttccgccgcc agccgttcga gcgacagatc ctcggacagg
    14821 tgctcatcaa tatagaaaca gagctgttca tacagcgaga gctgtgcttt ccgggtgagc
    14881 gggtgatgct gctcatacac cgttcggttg atctgcatca gcaggtcgct gacgtagaga
    14941 aaaatctgcg tctcttttcc aaaatgatcg gaccgcattt cctcaatcag tcggatggtg
    15001 cggtactgga tcgtgttgaa ctcgacggaa tcgagcggga acagatattt ctgattttcg
    15061 gcagcgtatt ccatcaggta aacgtagtcg gcggaacggg cgcggagctg ctctgcaaaa
    15121 gcggcgctga cccagaacac aaagcggcgg tacggcacag acaggtcgtg catcaccaga
    15181 tgatgcggca cgccgggcgg aatcagaaga agatcgccgg gcgtcggtgt gtagagcact
    15241 ccgtcgattt ccagtgagat cgccccttcg agaaagagat agaactcata atgcgtatgc
    15301 gtatgttctt ttacgccggg cacatcgtcg gtgctgtaat agtataactc aaacgtatcc
    15361 gaaatcatgt actgccgggt ggcaaatttc gtctgcagtt tttctttcat aataaaagca
    15421 tccttgcaaa aaaatgatat ttttgaacca taacaacaat tcccagcata tcacaaaaca
    15481 gcaagtttta caatgtattc ggcaattcgc acaatagaaa aaaacaactt gtccggttat
    15541 gatacaaggg aataaaataa aaacagaatg cagaataaat ttcagataaa gatcaaaacg
    15601 gaggaacagc tatcatgaat cagattctcg aacagcttgg aacatacggc attgtgccgg
    15661 ttgtcgtttt acaggatgca gcaaaagcag agccgcttgc cgaagctctc tgcaagggcg
    15721 gacttgcctg cgccgaagtg accttccgca ccgatgcggc agaagaatcc atccgcatta
    15781 tgagcgaaaa attcccggag atgctggtgg gagccggaac tgtgctcaca actgagcagg
    15841 cggaccgcgc cgtgaaagcc ggagcaaaat ttatcgtcag cccgggcctc aacccggagg
    15901 tagtaaaatg gtgccaggca cacgaagtac cagtgatccc gggcatcgtg accccgactg
    15961 agatggcgca ggcaatcggt cttgggctca ccatggtgaa attcttcccg gcagaaccgg
    16021 caggcggact gaaatacatc cgtgccatcg cagcaccgta caccatgatg aaattcatgc
    16081 cgaccggcgg catcaacccg cagaatgtaa gagaatacct ggcatacgac cgcatcgcag
    16141 catgcggcgg aagctggatg gtaaaaaata cgatgattga aaacaacgaa ttcgaccgca
    16201 tcgaagagct ggtgaaagag gcggttgaga tcgtaaagga aagccggacg tgacgacatc
    16261 cttccctcag ccgctgggca ccgaccgttg acctcgagcg tgatccgaga tggggaagaa
    16321 acgaggaggg ctacggcgag gaccctgtgc tgaccggaaa aatggccggc gcttacatcg
    16381 agggaatgca gggcgacgat ccaaaatatc tgcgctgtgc atcaaccctg aaacacttct
    16441 atggaaacaa caccgaggtc ggccgcggct ggaagaactc ttcgattgac ccgcggaata
    16501 aatatgagct gtatctcgag ccgttccgca gatgtattga ggagagcggt gcagagggca
    16561 tcatgacggc gtacaacaga atcaacggca ccgtcggcgg aatggtccgg gaaaacatgg
    16621 aaatctcgga atccgtcatc atgaaacagg cggaccagaa gatgtatgaa aataaaaaaa
    16681 gttcacggtg aatcgccgca atggattgtc cccgcgggtc ttttcgtggt acaatgaaac
    16741 ataaccgata aaaagaaaaa cgagggcaga acatgaacga attatatgaa atgattgaaa
    16801 agaaaatcaa agaatccggc tatccgcggc cgatttcggg aagcgatgtt tacgatgaca
    16861 tctgcgacca gatcgaggga aaagagaacg gttcctatgt ggtattatcg aaatttgatg
    16921 atgatgtgat tttcgagtac cacatcacgg tgatggatga tgaattcaac ctcggcgtgc
    16981 tgacgatgcg caccccggag ggcgtgtttg agactgattt tgaccagtaa aagagaaaag
    17041 aaagaggttg ttacttatgc cgaatgttga aaaaatcaca aaacagacgg aaaatccgtt
    17101 cgtaaatctt tacctggtgg aagggacaaa caaagtaaac catcacttcc gctattacgt
    17161 ggcttcccgc gcaaaatcca tcgagcagat gaagattaaa acaaggaaaa atacgccgga
    17221 cggtgtgctg atgtacagtc tgtacgggga aaaacgcgac aaaattgtcc tggtgcgcca
    17281 gtaccgcgtt tccattgatg actacatcta cgaattcccg gcaggcctgg tggaaccggg
    17341 cgaggagttc catgaggctg ccgtgcgcga gatgcatgag gagaccggtc ttacgtttca
    17401 cccgttgaaa gtagatccct gctacgagaa accgttcttc accacaatcg gcctgaccga
    17461 tgaagcatgc tctgccgttt acggctggtg cgagggcgag atcagccgca aatacctcga
    17521 agagagcgaa gtgatcgaag ttgtccttgc tgaccgcgag gaggcaaaga gaatcttaaa
    17581 ggaagaaaat gtagcaatta tgtgttctta tcatctgatg cattttattc atgatgagga
    17641 gccgtttgca ttcctgaaat aagtggaggg gagccagaaa aacacaaccc ctggaagttc
    17701 ttagcacgaa ctgccagggg ttgtgttttt ctgactccca tttacgttgt gggggacggg
    17761 agggtgagga ctcaggtgta gtattgggcc tgggtttcgt aaagattggc gtagatgccg
    17821 tggtttgcga ggagggtgtc gtgggttccg tcttcggcga tgcggccgtg gtcgaggac
//
LOCUS       DAOMUF010000023        17580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_616_length_17580_cov_4.255064, whole genome shotgun sequence.
ACCESSION   DAOMUF010000023 DAOMUF010000000
VERSION     DAOMUF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 17580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17580
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_616_length_17580_cov_4.255064"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLN77_02765"
     CDS             complement(<1..333)
                     /locus_tag="PLN77_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43237.1"
                     /translation="MAKNTYKQDEVLEEPFDIRHLLRASSYIKKYAGRMVVAILLSGI
                     GGAFGYVAPMIIQRALDLAIPDKNEKLLFSLVGMLVGIYVVSVIFTTIRSRIMVDVSQ
                     NIIYDIRKD"
     gene            complement(346..2133)
                     /locus_tag="PLN77_02770"
     CDS             complement(346..2133)
                     /locus_tag="PLN77_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43238.1"
                     /translation="MKSSTKPFPKTKIQWLWENMEGKRALYLIGMIGTVLYNVLQLTI
                     PYFSGKIVDLFLTGENAVQNLQTHRDLFYRLLIAMIGFTFLRVVIVYGSCMIYEHVSQ
                     SVLYRVRNYLYDKIQRQDMTFYSTYRTGDLMTRLTGDLDAVRHMVAWVIRMVIESFSL
                     FAAAAIFFLYTNWKLALCILVISPFVFWIIYLFRNRVAPMHAALREKLAQMNTCAQEN
                     ISGNRVVKAFAREEYEIEKFDRANTDYAETNKATAMVWLKFFPFVESIANLMPVVLLA
                     VGGVFMIKGQLTMGDYVAFSGLIWAIANPMRQMGNIMNEFQRFSAASKKVMELYYSEA
                     KIKDEENAVAHPERFEGKIEFDNVSFQYEDGDLPVLHNISFTVNPNETVAVMGETGCG
                     KTSLINLIPRFYEPTEGQIRIDGIPVEDLKLYDLRKHIGLATQDVLLYSDTIEGNIAY
                     GDDSIKMNEVIKFAKYSAAADFISKMPEGYDTQVGERGVGLSGGQKQRISLARALAIR
                     PSILILDDTTSAVDMETEKQIQHSLRELDFPCTKVIIAQRISTTKTADKIIVLQNGYI
                     KEMGTHEELMEKKGYYYELVKLQTGVDEL"
     gene            2343..3248
                     /locus_tag="PLN77_02775"
     CDS             2343..3248
                     /locus_tag="PLN77_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM43239.1"
                     /translation="MKNLFEYSDILRSPIEAFSCSSETFSMPVVSHWHYFTEVLYLQE
                     GSLLVSCNDVVYPMEAGSFILFPPQAVHALAADHDKPFRFFCVKFNLNRIHLTGSYLP
                     NLSFAFHKVASMTHPSLLFTQMDLPGLDLNDFFTTIEKESHAKQYGYDAYIYSLFSTL
                     FLRLLRTWHCQGICFDLETISESEEKSMQDILVYIDRHSQENINIEELAHKYNMSYSY
                     FAKLFLKHYGQSCKQYIEFIRLNKVENLLLFTDYDLNFIATETGFADCSHLIRTFKKR
                     YQITPRQFRQEHQSTVSSSLPAKLG"
     gene            complement(3360..4940)
                     /locus_tag="PLN77_02780"
     CDS             complement(3360..4940)
                     /locus_tag="PLN77_02780"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRM43240.1"
                     /translation="MVTWNNLDKLASFEELKKVERVNLAEVMSGENGAERVKSYSVPM
                     AEGLAYNYAAKQVNEDVLAALVKLAEEAQLSEKFEELYNGAVINTGENRLVLHQLTRG
                     QLGNTVTADGVDKRAFYVEQQNRIAEFANKVHAGEITNAAGEKFTTVVQIGIGGSDLG
                     PRAMYLALENWAKKNGCFKMEAKFISNVDPDDAAAVLNSIDVAHSIFVLVSKSGTTLE
                     TLTNESFVKDALKNAGLDASKHMIAVTSETSPLAKSDDYLAAFFMDDYIGGRYSSTSA
                     VGGAVLSLAFGPEVFAAFLEGAAAEDKLSANKDVLQNPEMLDALIGVYERNVLGYPST
                     AVLPYSQALNRFPAHLQQVDMESNGKSVNRFGEPVNYPTGPVLFGEPGTNGQHSFYQL
                     LHQGTDIVPLQFVGFRNNQMETDVDIQGSTSQQKLCANVAAQIVAFACGKADENKNKN
                     FEGGRPSSIIIGDQVNPKTLGALLAHFENKIMFQGFLWNVNSFDQEGVQLGKVLAKRV
                     LAHETDGALKVFSDLLNI"
     gene            complement(5063..6055)
                     /gene="pfkA"
                     /locus_tag="PLN77_02785"
     CDS             complement(5063..6055)
                     /gene="pfkA"
                     /locus_tag="PLN77_02785"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRM43241.1"
                     /translation="MATTNKVKTIGVLTSGGDAPGMNAAIRAAVRRGLQKGVQMKGIL
                     KGYDGLMNEEIIDMTARDVSDTIERGGTILYTARCAEMRTPEGQKRAADVCRKHGIDG
                     LVVIGGDGSFAGAQKLAALGINTVGVPGTIDLDIACTEYTIGFDTAVNTAMEAIDKVR
                     DTSTSHERCSIIEVMGRNAGYIALWCGIANGAEDILLPEKYDYDEQKIINNIIENRRK
                     GKKHHIIVNAEGIGHSTSMARRIEAATGIETRATILGHMQRGGSPTCKDRVYASIMGA
                     YAVDLLLEGKTDRVVAYKNGEYVDYDIDEALSMTKTLPEYQWEIARALSYNYTK"
     gene            complement(6137..9610)
                     /locus_tag="PLN77_02790"
     CDS             complement(6137..9610)
                     /locus_tag="PLN77_02790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRM43242.1"
                     /translation="MSFTHLHVHTEYSLLDGSNKIKEYVARVKELGMNSAAITDHGVM
                     FGCIDFYKEARAQGIKPILGCEVYVAPGSRFDRETSRGEDRYYHLVLLAENNTGYSNL
                     MKIVSAGFIDGFYYKPRVDMEILEKYHEGIIALSACLAGEVARAMVRNQYEMGKEAAL
                     RYEKIFGKGNFFLELQDHGISEQRMVNQQLMRLSAETGIELVATNDIHYTYAEDADSH
                     DILLCLQTGKKITDEDRMRYEGGQYFVKSEAEMASLFPYAPQAIENTQKIADRCNVEI
                     EFGVTKLPKFDVPEGYTSWEYLNKLCYDGLEERYHPATDELKARLKYELDTIKTMGYV
                     DYFLIVWDFIKFARDHDIMVGPGRGSAAGSIVSYTLGITQLDPMRYQLLFERFLNPER
                     VTMPDIDVDFCFERRPEVIDYVTRKYGKDRVVQIVTFGTLAARGVIRDVGRVLDMPYA
                     QVDSIAKMIPNELNITIDKALQMNHELRELYQGNEEVAHLIDMSRRLEGLPRHTSMHA
                     AGVVIGSRPLVEFVPLSRGSDGTIVTQFTMTTLEELGLLKMDFLGLRTLTVIQNATKL
                     AERYSNKKIDLLHIDYNDPKVLGMIGASKTVGVFQLESAGMKNFMKELKPQSLEDIIA
                     GISLYRPGPMDFIPQYIKGKNNPESVTYDTPLLKPILEPTYGCIVYQEQVMQIVQALA
                     GYSLGRADLVRRAMSKKKASVMEKERQNFVYGNEAEGVPGCIKNGIDEKTANKIYDEM
                     IDFAKYAFNKSHAAAYAFVSYQTAWLKYYYPVEFMAALMTSCIDNPGKVAEYILSSRQ
                     MGISILPPDINRSEGKFTVDGQAIRYGMAAVKGIGKPVMEAIVEERSANGPFRSLKDF
                     AERLSGKEVNKRTVENFIKAGAFDCFGVTRKQLMFVYANVLDDVAREKKDSLSGQMSL
                     FDFVDETTKRSYETVYPDVGEYEKSDLLALEKEVLGIYVSGHPLEEQEECWRKNISAV
                     TTDFQPDEETGFPSVTDGAKEIVGGIITDKKIKYTKNNKTMAFLTLEDLVGSMEIVVF
                     PRDYEKNAAMMQTDARVFIQGRVSAEDDKPSKLICEKIFPFAGIPKELWIQFPDKETY
                     EREVADLYQMLRESDGKDQVVLYIQSIRAMKKLPPSRNVQADARLVASLQAKFGTGNV
                     KVVEKSIEKRA"
     gene            complement(9622..12798)
                     /locus_tag="PLN77_02795"
     CDS             complement(9622..12798)
                     /locus_tag="PLN77_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM43243.1"
                     /translation="MKKKKSKKKTSFISKTAMTVLTIILIILFFCIMSMVEKIQGTAR
                     VVNYAGLVRGKTQRIIKLEDAGEPQDTMIADINAYIDGLQNGSSELDLVRLDDRDFQD
                     KMTELASYFEELKAEILLVREKGYENTAIIEKSETFFKICDEATGLAEAYSQRMASSL
                     KKLEQVVVGDIIGLVFVIGMELIKAVRYAAMNRILQKKVYLDEATGLPNKNKCEEILE
                     GSDGGEEISGVYAVCVFDLNNLRTINNSLGHDKGDEYIRSFAVQLRKAVPEEYFVGRN
                     GGDEFLAILRGLNREEVEACMNHIRTQTAEYSRQHPEMPISYAGGYALSTEFEDCDIR
                     ELFRHADQNMYIDKNRAKMEEAAAERKISLEALDVVKKKGYHFSNCIYCNARQDQYRI
                     LRAVSGFFLAEDGSYTGAAEHIVQGITDEEKRKEMRRMLDLTHLKECYQKGEESVEIL
                     CEYREGSEGEALCRGKVTILFYDAAEDGGLHHFLMGFERFRSDEEAARNEKEQLDQYY
                     EQMKQSIIENSHYAEALLETAEAVFAVDLTNDSLEQIFYHSGQKKFDLNIDLPCSYEK
                     YCGECSAFVAEETLESYRIVDSSKKLLQRFENGDKQVVVEYQETDESGRRIWLQKTVL
                     MSQDTVYDRKTKRESVVVHGMILFRDTSEFHSREEREKIQLQDAFRAADSASRAKTEF
                     MNRMSHDVRTPINGIMGMLDIIRKNRQDEAKVDDCLDKINLSASHLLALVNDVLDMNK
                     LESGKETIESASFDLTHLMDDVASLVNAQITEMGITHCTHRGELTHTRLIGSPLRLRQ
                     IMLNLFSNAIKYNKPGGMIDTYAGEVSCDGTRVVYEFRITDSGIGMSSEFIEKELFHP
                     FTQEKSGARTQYKGTGLGMSIVKELIEKMQGTIEVTSQQGVGTTYVFRLPFYIDCEGQ
                     RIQEVKTRIAGKELVRFHVLLVEDNEINMEIAEFYLTDHGATVEEAWNGREAVEKYAA
                     NPQKFDVILMDLMMPVMGGLEAARQIRTMELPEAKQVPIIPMTAQSSEESKEGCRAAG
                     MNSHLTKPIEPDSLVQTILETTEHAFGK"
     gene            complement(12976..13827)
                     /locus_tag="PLN77_02800"
     CDS             complement(12976..13827)
                     /locus_tag="PLN77_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HRM43244.1"
                     /translation="MLNIGRKQKLVVVKKVEFGIYLGETREAGEKERVLLPIKQVPEG
                     TKEGDELEVFLYRDSSDRLIATTREPKLVLGELAVLNVAATGKIGAFLDWGLEKDLFL
                     PFKQQTRRLHPGDEVLVSLYIDKSDRLCATMKVYHFLKTNSPYVVGDIVKGRIYEISG
                     NFGVFVAVDDRYSGLIPKREAQADYHVGQELECRVTEVKEDGKLSLSARGKAYEQIAI
                     DAESVLSVIEEFAGVLPFDDKASPEVIQREFGLSKAAFKRAVGHLMKEKQVVIRDGKI
                     WTAEKAK"
     gene            complement(13841..15310)
                     /locus_tag="PLN77_02805"
     CDS             complement(13841..15310)
                     /locus_tag="PLN77_02805"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRM43245.1"
                     /translation="MRAQNLTLLTDLYELTMMQGYYENPSDQIVVFDAFYRKNPCGGA
                     YAVCAGLEQVIEYVRDLHFSPDDIDYLRSLHIFNDDFLEYLRGFHFTGDIYAIPEGTV
                     VFPREPLLKVVAPIMEAQLVETAILNMINHQSLIATKASRVVYAAKGDGIMEFGLRRA
                     QGPDAGIYGARAAMIGGCIGTSNVLTGQMFDVPVKGTHAHSWIMSFPDEYTAFKTYAK
                     MYPEACILLVDTYDVLESGVPNAIRVFKEMRAEGLPMKGYGIRIDSGDLAYLSKKAYQ
                     MLEAEGFGDAIISASSDLDEYLIDSLKTQGAKINSWGVGTNLITSGDWPAFGGVYKLA
                     AAKDKDDAEFTPKIKLSENSEKVTNPGNKTIYRIYDKETGKIRADLICLADETFDESK
                     DMIIFDPIETWKKTKIKGGTYTLRELLVPIFQKGACVYTSPSVMEIRDICTREKDTLW
                     EESKRLVNPHEVYVDLSDKLYHIKSDLLEEMSMKALGEQ"
     gene            complement(15377..>15660)
                     /locus_tag="PLN77_02810"
     CDS             complement(15377..>15660)
                     /locus_tag="PLN77_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRM43246.1"
                     /translation="DAAPRLEDFPYRLSDNVRFADLDPNHHVNNAQFISLAGECLPKN
                     FTVRRMRAEYKQQAHLGDVLHPLRAETENGCFISLNDEKGQPYVVVEFQ"
     assembly_gap    15661..15670
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15671..16138)
                     /locus_tag="PLN77_02815"
     CDS             complement(<15671..16138)
                     /locus_tag="PLN77_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRM43247.1"
                     /translation="MKYSFTSRIRYSEIGEDGNLTLPGLINYFQDCSTFQSEAIGEGV
                     AELKKRGCAWVLSAWQIHVRRYPSLGEAVKITTWASGFKGFLGMRNFTMETEDGEMLA
                     CANSIWSYVNMETGHPVRAPKETTDAYGIDDPIDEDFGERKVKMPEADFIGEPF"
     gene            complement(16217..16288)
                     /locus_tag="PLN77_02820"
     tRNA            complement(16217..16288)
                     /locus_tag="PLN77_02820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16254..16256),aa:Gln,seq:ctg)
     gene            complement(16424..>17580)
                     /locus_tag="PLN77_02825"
     CDS             complement(16424..>17580)
                     /locus_tag="PLN77_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRM43248.1"
                     /translation="FVKENENTDNADVRTRYGILSSMVGICCNVLLFAAKLLIGVGMH
                     SLAIMADAFNNLSDAASSVISFVGVKMANKPADKEHPFGHGRIEYIAALIVSFLVIEV
                     GFTFLKSSLEKIRNPEEITFQMVPFLILVLSVGVKLWMALFNRKLGNRIQSKVMLATS
                     ADSMGDVITTSATIASTLICHFLSVNIDAIAGLVVAVLVMWSGISIAKDTLEPLIGQG
                     ADPDLYARIMKEVASYEGIVGTHDLIVHNYGPGRSMASIHAEVPRDVDIEVSHDIIDR
                     IEHEVGEKLGILLVIHMDPVEIHDATVLATKEKVRNVISILDPELSFHDFRMINGAEK
                     KKLIFDLEIPYSYKKKDCDRVIGQIGALMKEMDEKYECVVNIDKKYVAGE"
BASE COUNT         3843 a         4833 c         4090 g         4804 t
ORIGIN      
        1 gtcttttcgg atatcgtaga tgatattctg gctgacatcc accatgatac ggcttcggat
       61 cgtcgtgaaa atgacgctga caacgtagat gccgacgagc attccgacca gagaaaacag
      121 aagtttctcg tttttgtccg gaatcgcaag gtccagtgcc cgctggataa tcatcggcgc
      181 aacgtagccg aacgcgccgc cgatgccgct taagagaatc gccacaacca tccggcccgc
      241 gtatttttta atataagatg atgcccggag taagtgtctg atgtcaaaag gttcttcgag
      301 cacttcatcc tgcttgtatg tattttttgc cattgcttcc ccctcctaca gttcatccac
      361 accggtctgc agtttcacca gttcgtagta atagcccttc ttttccatca gctcctcgtg
      421 cgttcccatc tccttgatgt aaccgttctg cagcacgata attttgtccg ccgtctttgt
      481 ggtggagatt cgctgcgcga tgataacttt cgtgcacggg aaatccagtt ctcgtaagga
      541 atgctggatc tgtttttccg tctccatatc gacggccgat gtcgtgtcgt caagaatcag
      601 gatcgacgga cggatggcca gcgctctcgc cagggaaatt ctctgcttct ggccgcctga
      661 aagaccgacg ccgcgctcac cgacctgagt gtcatagccc tccggcatct tcgaaataaa
      721 gtctgccgcg gcagaatatt ttgcaaattt aatgacttca ttcatcttga tcgagtcatc
      781 gccgtaagca atgttgccct cgatcgtatc agaatacagc aggacatcct gcgtcgcaag
      841 tccgatgtgt ttccggaggt catacagctt cagatcttcc accggaatac cgtcgatgcg
      901 gatctggcct tctgtcggct cgtagaaacg cggaatcagg ttgatgaggg atgtctttcc
      961 gcatcccgtc tctcccatga ccgccacggt ctcattcggg ttaaccgtaa acgaaatgtt
     1021 atgcagaacc ggcagatcac cgtcctcata ctggaacgaa acgttgtcga actcgatttt
     1081 tccctcgaag cgttccgggt gtgccactgc attttcctcg tccttgatct tcgcctcgga
     1141 gtaatacagc tccatgactt tttttgaagc cgccgagaac cgctggaact cgttcatgat
     1201 atttcccatc tggcgcatcg ggtttgcgat tgcccagatg aggccggaga atgccacata
     1261 atcacccatg gtcagctggc ccttaatcat aaagacaccg ccgactgcca gcaggacaac
     1321 cggcatcagg ttagcgatac tctcgacaaa cgggaagaat ttcagccata ccatcgcggt
     1381 tgctttgttt gtctccgcgt aatcggtatt agcgcggtcg aatttctcga tctcgtactc
     1441 ttctcttgca aacgccttga cgacacggtt tccggagata ttctcctgcg cacacgtatt
     1501 catctgtgcc agcttttcac gcagtgccgc atgcatcggg gcaacacggt tgcggaacag
     1561 gtagatgatc cagaatacaa acggcgaaat cacaagtatg cagagcgcca gcttccagtt
     1621 cgtgtacagg aagaaaatgg ccgctgccgc gaacagggaa aacgactcaa tcaccattcg
     1681 gatgacccat gcgaccatat ggcggacggc atcgaggtct ccggtcagac gcgtcatcag
     1741 atcgccggta cggtacgtgc tgtaaaacgt catatcctgc cgctggattt tgtcatacag
     1801 gtaattccgc acccggtaca ggacgctctg tgatacgtgc tcgtaaatca tacagctgcc
     1861 gtagacaata acgacacgca gaaacgtgaa gccaatcatc gcaatcaaca ggcggtaaaa
     1921 cagatcccgg tgtgtctgca gattctgcac tgcattttca cctgtcagaa acaggtcaac
     1981 aatttttccc gaaaaatatg gaatcgtaag ctgcagaacg ttatataata ccgttccgat
     2041 catgccgatc agatacagcg cacgctttcc ttccatgttt tcccacagcc attggatttt
     2101 ggttttcggg aatggttttg tgctgctttt cattttttca tatcccccct tatcttctga
     2161 aaatcaaaag gtttctgcat ttttctcttt caaatgcttt cgccattgta tcaattttca
     2221 tttcattatg atataatgcg actgtataaa aatagaacgt ttctatcttc atgcatgatt
     2281 cttttgaata atcctcgtaa ttttttgcag aactttttaa ccggaacagg aggaatttac
     2341 ttttgaaaaa tctatttgaa tacagtgata tcctgcgctc accgatcgag gcattttcct
     2401 gcagctcgga aaccttttcc atgccggtgg tatcacactg gcactatttt actgaagtac
     2461 tttatctgca ggaaggctcg ctccttgtga gctgcaacga tgttgtctat ccgatggagg
     2521 ccggctcttt catccttttt ccgccgcagg cagtccatgc gcttgccgcg gatcatgaca
     2581 aaccgtttcg ctttttctgc gtaaaattca atctgaaccg gattcatctc accggaagct
     2641 atcttccgaa cctgagcttt gcgttccata aggttgcctc catgacgcat ccttccctgc
     2701 tctttacgca gatggatctt ccggggctcg acctgaacga ttttttcaca accattgaaa
     2761 aagaatccca tgcgaaacag tacggctacg acgcgtatat ttattcgctg ttttcgacac
     2821 tgttcctgcg ccttctgcgg acgtggcact gccagggaat ctgttttgat ctcgaaacca
     2881 tctccgagag tgaagagaaa agtatgcagg acattctcgt ctacatcgac cgtcattcac
     2941 aggagaatat caacatcgaa gagctggcgc ataaatacaa tatgagctat tcgtactttg
     3001 ccaagctgtt tttaaaacac tatgggcaga gctgcaagca gtatatcgaa tttatccgtt
     3061 tgaacaaggt cgagaacctg ctcctcttta ccgattatga cctgaacttt attgccaccg
     3121 agaccggctt tgcagactgc agccacctga tccgtacgtt caaaaaacgg tatcagatca
     3181 ccccgcggca gttccggcag gagcatcagt ccacagtgtc ctcgtcgctt ccggcgaagc
     3241 tgggataacc ccataatata gtaataaaaa aggcggcata ccgttttccc cctgaaactt
     3301 tcagaaagaa aatccggtat gccgcctttt catatcttaa attttctgat ttaaacttct
     3361 tagatgttga gcagatcgct gaatactttc agtgctccgt ctgtctcgtg agccagaacg
     3421 cgttttgcaa ggactttacc aagctgtacg ccttcctggt cgaagctgtt tacgttccac
     3481 aggaatccct ggaacatgat cttattctcg aagtgtgcaa gcagtgctcc cagcgtcttc
     3541 ggatttacct gatctccgat gatgatgctg gacggacggc cgccttcgaa gtttttgttt
     3601 ttattctcat ctgctttacc gcaggcaaat gcaacgatct gagctgctac gtttgcgcag
     3661 agtttctgct ggctggtgct gccctggatg tcgacatcgg tctccatctg gttattgcgg
     3721 aagcctacaa actgcagcgg aacgatatca gttccctgat gcagcagctg atagaaggaa
     3781 tgctgtccgt tggtacctgg ctcgccgaag agaaccggac cggtcgggta attgaccggc
     3841 tcgccgaagc ggttgaccga tttaccgttg gactccatat ctacctgctg taagtgagcc
     3901 gggaaacggt tcagtgcctg ggagtacgga agaactgcag tgcttggata gccaagaacg
     3961 tttctctcgt aaacaccgat cagcgcatcg agcatctccg ggttctgtaa tacgtctttg
     4021 tttgcagaaa gtttatcttc tgctgcagcg ccctcgagga atgctgcgaa tacttccggt
     4081 ccgaaagcca gggacagaac agcgccgccg actgcggatg tggaagaata acggcctccg
     4141 atgtagtcat ccatgaagaa tgctgccaga tagtcgtcgc tctttgccag cggagaagtc
     4201 tcactggtaa cggcgatcat gtgcttggat gcatccaggc ctgcattttt cagagcgtct
     4261 tttacgaagg actcgttggt cagagtctcc agtgtggttc cggatttgga taccagaacg
     4321 aagatggaat gtgctacgtc gatcgaattc agaacagctg ctgcgtcatc cgggtcaacg
     4381 ttgctgatga attttgcttc cattttgaag cagccgtttt tctttgccca gttttccaga
     4441 gccagataca tcgcacgcgg gccaaggtcg cttccgccga taccgatctg cacaacggtg
     4501 gtgaattttt cacctgctgc gttggtgatc tcacctgcat gtactttgtt cgcgaactct
     4561 gcgatgcggt tctgctgctc tacgtagaaa gcacgtttgt ctacgccgtc tgccgtaacg
     4621 gtgtttccga gctggccgcg ggtcagctga tgaagcacga gacggttctc tccggtgttg
     4681 atgacagcac cgttgtaaag ctcctcaaat ttttcagaaa gctgtgcctc ttctgccagt
     4741 tttacgagtg cggcaaggac atcctcattc acctgttttg ctgcgtagtt atacgcaagt
     4801 ccttctgcca tcggaacgct gtaagatttt acgcgctctg ctccgttttc gccggacata
     4861 acctcggcaa gattgacacg ctccactttt ttcagctcct caaaagaagc cagtttatca
     4921 agattgttcc aggtaaccat tgttaagttc ctcctgtttt ttattttgtc tctctattcc
     4981 gtccgcggaa acatttttat tcccgctatt ataccaatga aacagactgc ttgctaaaaa
     5041 cagtctgttt cgtgatattt ttttatttcg tatagttata agaaagagct cttgcgatct
     5101 cccactgata ttccggcaga gtctttgtca tggacagtgc ctcatcgata tcgtaatcta
     5161 catactcacc gttcttgtat gcaacaacgc ggtctgtttt gccttccaga agaaggtcaa
     5221 ctgcgtaagc acccatgatg gacgcatata cacggtcttt acatgtcggg cttcctccac
     5281 gctgcatatg tccaaggatc gttgcacggg tctcgatgcc ggttgcagct tcgattctgc
     5341 gcgccatgct ggtggaatgg ccgatgccct cagcatttac gatgatatga tgttttttgc
     5401 cttttctacg gttctcgatg atgttgttga tgatcttctg ctcatcgtaa tcgtatttct
     5461 ccggaagaag aatatcctct gcgccgtttg cgataccgca ccacagagcg atatatccgg
     5521 cattacgtcc cataacctcg atgatcgagc atctctcatg ggaagtggaa gtatctctta
     5581 ctttatcaat ggcttccatt gcagtgttta ccgcggtatc aaaaccgatg gtatattctg
     5641 tgcaggcgat atccaggtcg atggttcccg gtacacctac ggtgttaatt ccaagagctg
     5701 ccagtttctg cgctcctgca aaagatccgt ctccgccgat aactaccagt ccgtcgatgc
     5761 cgtgttttct gcagacatct gcagcacgtt tctggccttc cggtgtacgc atctctgcgc
     5821 agcgggcggt ataaaggatg gtacctccgc gctcgatggt gtcggataca tcgcgggctg
     5881 tcatgtcgat gatttcttcg ttcatcagtc cgtcgtagcc tttcaggata cctttcatct
     5941 gaacgccctt ctgcagacct cttcttacgg ctgcacggat cgctgcgttc atacctggtg
     6001 catcaccacc gctggtaagc actccgattg tctttacctt gttagtcgtt gccatatgaa
     6061 ttcctccatc tcatcacaca cattaataaa cgagtagttt tatttattaa tcacacacag
     6121 ttttatttta aagcattcag gccctttttt caatactctt ttctacaact ttcacgtttc
     6181 cggtaccgaa ttttgcctga agtgacgcca ccagacgggc atctgcctgc acattgcggc
     6241 tcggcggcag ttttttcatt gctctgattg actggatgta gagaacaacc tgatctttgc
     6301 catcggattc gcgcagcatc tgatacaaat ccgcgacttc gcgctcgtac gtttccttgt
     6361 ccgggaactg gatccagagc tctttcggga tgcctgcaaa cgggaaaatt ttctcgcaga
     6421 tcagcttgct cggcttatca tcctccgcgg aaacacggcc ctggatgaag acacgcgcat
     6481 cggtctgcat catcgctgcg tttttctcgt aatcgcgcgg gaatacgacg atttccatac
     6541 tgccgacgag gtcctcgagt gtgagaaatg ccattgtctt gttattcttt gtatatttga
     6601 tttttttatc cgtgataatg ccgccgacaa tctctttcgc gccgtcggtg acggatggga
     6661 aacccgtctc ctcatccggc tggaagtctg tcgttaccgc ggaaatgttc ttccgccagc
     6721 actcctcctg ctcctcgagc ggatgaccgc tgacgtagat gccgagcacc tctttttcga
     6781 gcgccaaaag atcactcttt tcgtattctc cgacatccgg gtacaccgtt tcatacgacc
     6841 gtttggtcgt ctcatctacg aagtcaaaca gactcatctg gccgcttaac gaatcttttt
     6901 tctcgcgcgc cacatcatcg agcacattgg cgtagacgaa catgagctgc tttctcgtca
     6961 cgccgaagca gtcgaatgcg cccgccttga taaagttctc gacggtccgc ttgttgactt
     7021 cttttccgct caggcgctcc gcgaaatcct ttaacgagcg gaacggtccg tttgcactcc
     7081 gctcctccac gatcgcctcc atgaccggct ttccgatgcc tttgaccgcc gccatgccat
     7141 agcggatcgc ctggccgtcc accgtgaatt ttccttcgct tcggttgatg tccggcggca
     7201 gaatggaaat gcccatctgg cgggaactca agatatactc tgcgactttt cccgggttat
     7261 cgatgcagga ggtcatcaga gccgccatga actcaaccgg gtaatagtat ttcagccatg
     7321 ccgtctggta cgatacgaac gcgtacgccg ccgcatggga tttgttgaag gcgtatttcg
     7381 cgaagtcgat catctcgtcg tagatcttgt tcgctgtctt ctcatcaatg ccgtttttga
     7441 tacagcccgg cacaccctcg gcctcgtttc cataaacgaa attctggcgc tctttttcca
     7501 tgacggatgc ctttttcttc gacatcgcac ggcgcaccag atcggcccgt ccaagcgaat
     7561 agcccgccag tgcctgtacg atctgcataa cctgttcctg gtagacgata cagccgtacg
     7621 tcggctccag aatcggtttt aagagcggtg tatcgtacgt aacggattcc gggttgtttt
     7681 ttcctttgat gtactgcgga ataaaatcca tcgggcccgg ccggtacagg gaaattccgg
     7741 cgatgatatc ctcgaggctc tgcggtttta actccttcat gaagtttttc attcccgcac
     7801 tctcgagctg gaacacaccg accgttttcg atgcgccgat catgccgagc actttcggat
     7861 cgttgtaatc gatgtgaagc agatcaattt tcttattgct gtaacgctct gcgagctttg
     7921 tcgcattctg aatgacggtc agggtccgaa gccctaagaa gtccattttc agaagtccca
     7981 gttcctcgag cgtcgtcatc gtgaactgcg tgacgatcgt tccgtcgctt cctcgcgaga
     8041 gcggcacaaa ttccaccagc ggacgcgagc cgatgacaac accggcggcg tgcatcgacg
     8101 tgtggcgcgg cagaccttca agtctccgtg acatgtcgat cagatgtgca acctcttcat
     8161 tgccctggta cagctcccgc agctcgtggt tcatctgcag tgccttgtcg atcgtgatat
     8221 tcagctcatt cggaatcatc ttcgcgatcg aatcgacctg tgcatacggc atatccagca
     8281 cacggccgac atcgcggatg acaccgcgcg ccgccagtgt accgaatgtg acgatctgta
     8341 caacgcggtc cttgccgtat tttctcgtta catagtcgat gacctccggg cggcgttcga
     8401 agcagaaatc cacgtcgata tcaggcatgg taactcgttc cgggttcagg aaacgctcga
     8461 acaggagctg gtaccgcatc ggatccagct gtgtgatgcc aagggtgtag gaaacaatac
     8521 ttccggccgc cgatcctcgt cccggtccga ccatgatgtc gtggtcgcgc gcaaatttga
     8581 tgaaatccca cacgatcagg aagtaatcga cgtaacccat cgttttgatc gtatcaagct
     8641 cgtatttcag ccgcgctttc agttcatccg tcgccgggtg ataccgttcc tcgaggccgt
     8701 cgtagcagag cttatttaag tattcccagg aagtgtatcc ttccggcaca tcgaatttcg
     8761 gaagttttgt gacaccaaat tcgatctcca cattacaccg gtctgcaatt ttctgtgtat
     8821 tttcgattgc ctgcggtgca tacgggaaga gcgatgccat ctccgcctcg gatttcacga
     8881 aatactggcc gccctcgtag cgcatgcggt cctcatccgt gatcttcttt ccggtctgca
     8941 ggcacagaag aatatcgtgg gaatccgcat cttctgcgta cgtgtagtga atatcattcg
     9001 tcgccaccag ctcgatgccg gtttctgccg agaggcgcat cagctgctga ttgaccatcc
     9061 gctgctccga gatgccgtga tcctgcagtt ccaggaagaa attgcctttt ccgaaaatct
     9121 tctcataccg cagtgcggcc tcttttccca tctcgtactg gttccgcacc atcgcacgcg
     9181 ccacctcacc tgcaaggcag gcggaaagcg cgataatgcc ctcgtgatac ttttccagaa
     9241 tctccatgtc gacacgcggc ttatagtaaa atccatcgat aaagcccgcc gaaacaatct
     9301 tcatcagatt ggaatagccc gtattatttt ccgccagcag aaccagatgg taataccggt
     9361 cctctccgcg gctggtttcg cggtcgaaac gggaacccgg cgccacgtaa acctcacagc
     9421 cgaggatcgg cttgatgccc tgcgcgcggg cttctttata aaaatcgata cagccgaaca
     9481 tgacgccgtg atcggtgatc gcggcgctgt tcatgccaag ctccttgact ctggcaacgt
     9541 actctttgat cttgttcgat ccgtccagaa ggctgtattc ggtatgtaca tgtaaatgcg
     9601 taaaactcat cgttttctcc tctattttcc aaaggcatgt tccgttgtct ccaagatcgt
     9661 ctgcaccagg ctgtccggct cgatcggctt ggtcagatgg ctgttcatgc ccgctgcgcg
     9721 gcatccctct ttgctctctt cggaggactg cgccgtcatc gggatgatcg ggacttgttt
     9781 tgcttccgga agctccatgg tgcggatctg ccgcgctgcc tcaaggccgc ccatcacagg
     9841 catcatcaga tccattaaaa tcacatcaaa tttctgcgga ttcgccgcat acttttcgac
     9901 cgcctcgcgg ccgttccatg cctcttccac cgtcgcaccg tgatcggtca ggtaaaactc
     9961 ggcgatttcc atgttgatct cattatcctc gacaagcaga acgtggaagc ggacaagctc
    10021 ttttcctgcg atccgtgtct ttacttcctg aatccgctgt ccctcgcagt caatgtaaaa
    10081 cggaagccgg aatacatacg tcgttccgac gccctgctgg ctcgttacct cgatcgtgcc
    10141 ctgcattttt tcgatcagtt ctttgacgat cgacatgcca aggcccgttc ccttgtactg
    10201 cgttctcgca ccggacttct cctgtgtgaa cggatggaac agctcttttt cgataaattc
    10261 gctgctcatt ccgatgccgc tgtccgtgat gcggaactcg tagacgaccc gggttccgtc
    10321 gcaggagact tcgcccgcat acgtatcgat cattccgccc ggcttgttgt acttgattgc
    10381 attgctgaag agattcagca taatctgccg caggcgcagc ggactgccga tcagtctcgt
    10441 gtgcgtcagc tcaccgcggt gcgtacagtg ggtaatgccc atctccgtaa tctgcgcatt
    10501 gacaagggat gccacatcat ccatcagatg cgtcaggtca aaagaagcgc tctcgatcgt
    10561 ctcttttccg gactccagct tattcatatc gagcacatcg ttgacgagtg ccagcagatg
    10621 gcttgcggaa agattgattt tatcgaggca gtcgtccact tttgcctcat cctgccggtt
    10681 tttgcggatg atatccagca tgcccataat gccattgatc ggcgtgcgca catcgtggct
    10741 catgcggttc ataaattctg tttttgcacg acttgcactg tctgcagctc taaaggcatc
    10801 ctgaagctgt atcttctccc tctcttcccg gctgtggaac tctgacgtat cacggaaaag
    10861 gatcatgccg tgtaccacaa cgctctcccg ctttgtcttt cggtcgtaga cggtatcctg
    10921 tgacataaga accgtcttct gcaaccatat tcttctgccg gactcatctg tttcctgata
    10981 ctccacgact acctgcttat ctccgttttc aaagcgctgc agcagtttct tcgaggaatc
    11041 cacaatgcgg tatgattcca atgtctcctc cgcgacaaac gcactgcatt ccccgcagta
    11101 tttctcgtag gaacacggca gatctatatt gaggtcaaat ttcttctgcc cgctgtggta
    11161 aaaaatctgc tccagcgaat cattcgtcag atcaacggcg aacacggcct cggccgtctc
    11221 gagaagtgcc tcggcgtagt ggctgttttc aatgatcgac tgtttcatct gttcatagta
    11281 ttggtcgagc tgttcttttt cattccgagc agcttcctca tcggagcgga accgctcaaa
    11341 gcccatcaga aaatggtgca gtccgccgtc ctcggccgcg tcgtagaaca gaatggtgac
    11401 ttttccccgg cagagcgctt ccccttcgct gccctcccga tactcgcaca gaatttctac
    11461 gctctcttct cccttctgat agcactcctt cagatgggta agatccagca ttctacgcat
    11521 ttcctttctc ttttcctcat ccgtgatgcc ctgcacaata tgctcggccg caccggtata
    11581 gctgccgtcc tcggcaagga aaaaacccga aacggcgcgc agaatccggt actggtcctg
    11641 tcttgcattg cagtaaatac agttggaaaa atggtatccc tttttcttta cgacgtccag
    11701 cgcttccagg ctgatcttcc gctctgcggc ggcttcttcc atcttcgcgc ggtttttgtc
    11761 gatatacatg ttctggtccg catggcggaa cagttcccgg atatcacagt cttcaaattc
    11821 cgtggacagt gcatagcctc cggcgtagct gatcggcatc tccgggtgct ggcgggaata
    11881 ttctgccgtc tgcgtgcgga tgtgattcat gcacgcttcc acttcttcgc ggttcagtcc
    11941 gcgcagaatg gcaagaaact catctccgcc gtttcttccg acgaaatact cctccgggac
    12001 tgcttttcga agctgaacgg caaaggaacg gatatattcg tctcccttgt cgtgacccag
    12061 actgttgttg atcgtgcgca gattgttgag gtcaaacaca cagaccgcat acacgccgga
    12121 aatttcttct cctccgtcgc ttccctccag aatctcttcg catttatttt tatttggcag
    12181 gcccgtcgcc tcatcgagat agactttctt ctgcagaata cggttcatcg ccgcatagcg
    12241 gacggccttg atcagctcca tgccgatgac aaacaccaga ccgatgatat ctcctactac
    12301 cacctgctcc agcttcttca gcgaagacgc cattctctgc gaatacgctt ccgcaaggcc
    12361 cgtcgcctcg tcacaaattt tgaaaaaggt ctcactcttt tcgataatcg cggtattctc
    12421 atatcctttt tcacgcacca gcagaatctc tgccttcagc tcttcaaaat acgatgccag
    12481 ctcagtcatc ttatcctgaa aatcccggtc atccaggcgc accagatcca gctcgctgct
    12541 tccgttctgc agaccgtcga tataagcgtt gatgtctgca atcatcgtat cctgcggttc
    12601 tcccgcatcc tccagcttga taatccgctg cgtttttccg cgtaccagac cggcatagtt
    12661 gacaacgcgg gccgttccct gtattttctc gaccatcgac ataatgcaga aaaacagaat
    12721 aatcagaatg atcgtcaaaa ccgtcattgc ggttttggaa ataaaggatg tttttttctt
    12781 acttttcttt ttcttcatcg ctctccatca gatcctgtgt cattttttgt catatagatc
    12841 atattataag cgcaatcctc tcttcgcgca accgcaaacc gggcgcagaa aaaatttcaa
    12901 aattttatct gctgtctgac acaaaaaaac ggacaccgga gaggatgtgc ttcctgtcca
    12961 gtgcccgatc ttttcttatt ttgctttttc tgccgtccag attttaccgt cgcggatgac
    13021 aacctgtttt tctttcatca gatggccgac cgcgcgctta aatgctgctt tgctgaggcc
    13081 gaattctctc tgaatcactt ccggtgatgc tttgtcatca aacggaagga cacctgcaaa
    13141 ttcctcgatc acagaaagaa cgctctctgc atcgattgcg atctgctcgt acgcttttcc
    13201 gcgtgcggac agggagagtt ttccgtcctc tttgacttct gtaacgcggc actcaagttc
    13261 ctggccgaca tgatagtctg cctgtgcttc tctctttgga atcaggccgg agtagcggtc
    13321 atcaacagca acgaaaacgc cgaagtttcc gctgatctcg tagattcttc cctttacgat
    13381 atcgcctacg acgtacggag aattggtttt caggaaatgg taaaccttca tcgttgcgca
    13441 gagacggtcg ctcttgtcga tgtagaggga aaccagaacc tcatcacctg gatggaggcg
    13501 gcgggtctgc tgtttaaacg gaagaaacag atctttttcc agaccccagt ccagaaatgc
    13561 tccgatcttt ccggtagcag ctacgtttaa gacagccagc tctccaagca ccagcttcgg
    13621 ctctctggtt gtcgcaatca gacggtcgct ggaatcgcgg tacagaaata cttccagctc
    13681 atctccctct tttgttcctt ccggcacctg tttgatcggc agaagaacgc gttccttctc
    13741 gcctgcttcc cgggtctccc caagataaat accgaattct actttcttta ctactaccag
    13801 cttttgcttt cttcctatat ttaacatttg acttcctttc ttactgctcg ccgagagcct
    13861 tcatgctcat ctcctcgagc aggtcgctct tgatgtggta cagcttgtcg gacagatcca
    13921 cgtaaacctc atgcggattg accagacgtt tggactcctc ccagagggta tctttctctc
    13981 tggtgcagat gtcgcggatc tccatgacgg atggagaagt gtagacgcat gcgcccttct
    14041 ggaaaatcgg aacgaggagc tcgcgtaagg tgtaggttcc gcctttgatc ttcgtctttt
    14101 tccaggtctc gatcgggtcg aagatgatca tatctttgct ctcgtcgaag gtctcgtctg
    14161 cgaggcagat caggtcggca cggatctttc cggtttcttt atcataaata cgataaatcg
    14221 tcttattgcc cgggtttgtt actttctcgg agttctcgga cagcttgatc ttcggtgtga
    14281 attctgcatc atctttatct tttgctgctg ccagcttgta aacgccgccg aatgccggcc
    14341 agtcaccgga tgtgatcagg ttggtgccga cgccccagga attgatcttc gcaccctggg
    14401 ttttcaggct gtcgatcagg tactcatcga ggtcactgga tgcggaaatg atggcatcgc
    14461 cgaaaccttc tgcctcgagc atctgatatg cttttttcga caggtaagcc aggtcaccgc
    14521 tgtcgatgcg gatgccgtag cccttcatcg gtaagccctc tgctctcatc tctttgaaga
    14581 cgcggatcgc attcggaaca ccggattcga gaacatcgta ggtgtctacg agaagaatgc
    14641 aggcttccgg atacattttc gcatacgttt tgaatgcggt gtactcatcc gggaagctca
    14701 tgatccagct gtgtgcgtgg gttcccttga ctggaacgtc aaacatctgt ccggtcagga
    14761 cattggaggt tccgatgcag ccgccgatca tggcagcacg cgcgccgtag ataccggcat
    14821 ccggtccctg tgcacggcgg agtccaaact ccatgatgcc gtcgcccttt gccgcgtaaa
    14881 caacgcggga tgctttcgtc gcgatcaggc tctgatggtt gatcatgttc agaatcgcgg
    14941 tctctacaag ctgtgcctcc atgatcggag cgacaacttt taagagcggt tctcttggga
    15001 atacgacggt tccttccggg atcgcataga tatctccggt gaaatggaag cctcttaaat
    15061 actccaggaa atcatcgttg aaaatatgga gggaacggag atagtcgatg tcatccggtg
    15121 aaaagtgaag atcgcggaca tactcgatta cctgctcaag tcctgcacat acggcgtatg
    15181 cgccgccgca tggattcttt ctgtaaaatg catcaaatac cacgatctga tcggacgggt
    15241 tctcgtagta gccctgcatc atggtaagtt catataaatc ggttaacagt gtaaggttct
    15301 gcgctctcat ggcaaattcc cctttcctca ttgtgtttat tacgttcgta tcttattctt
    15361 tccgattgta ccacaatcat tgaaattcca caactacgta tggctgtcct ttttcatcgt
    15421 tcagcgagat aaaacagccg ttttccgtct cggcgcgcag cggatgcagg acgtcaccga
    15481 ggtgcgcctg ctgtttgtat tcggcgcgca tccggcgcac ggtgaagttc ttcggaaggc
    15541 attcaccggc aagggaaatg aactgtgcgt tgttgacgtg gtggttcggg tcgaggtcgg
    15601 cgaacctcac attgtccgac aggcggtagg gaaaatcttc cagacggggg gcagcgtcgg
    15661 nnnnnnnnnn gaacggttcg ccgatgaaat ctgcttccgg cattttcact ttgcgctcgc
    15721 cgaaatcctc gtcgatcgga tcgtcaatgc cgtaggcgtc tgtcgtctct ttcggtgcgc
    15781 ggacgggatg acccgtttcc atattgacgt aagaccagat ggagttggca caggcgagca
    15841 tttcgccgtc ttctgtttcc atcgtgaagt tacgcatgcc aagaaatcct ttgaagccgc
    15901 tcgcccatgt agtgattttt accgcttcac cgagggatgg atagcggcgc acatgaatct
    15961 gccacgcgga cagcacccag gcgcagcctc gttttttgag ctccgcaacg ccctcgccga
    16021 tcgcttccga ctggaaggta ctgcagtcct gaaagtaatt gatcagcccc ggcagggtga
    16081 gatttccgtc ctcaccgatt tcgctgtagc ggattctgct cgtaaaggaa tacttcatcg
    16141 tttcgttcct cctgctgtat ttgtttttga gcatcgaaaa aggcctcagg tacgagacct
    16201 gagacctttc aatgagctgg gctacctgga ttcgaaccag gaactgacgg agtcagagtc
    16261 cgttgcctta ccgtttggcg atagcccatt gccttgcgac aagatgtata atacactatc
    16321 gcaagagaaa agtcaacacc ttttttgaaa tttttttatt tttttcaaaa aactgacttt
    16381 tggaacattt tggaagggat ttgacggctc aacccggccg ttgtcactct cccgccacgt
    16441 attttttgtc gatgttgacg acacattcat acttctcatc catttccttc atcagcgccc
    16501 cgatctgccc tatcacgcga tcacagtctt tcttcttata agaatacggg atttcaagat
    16561 caaaaatcag cttctttttc tccgccccgt tgatcatacg gaaatcgtgg aaggaaagct
    16621 ccggatcgag aatggaaatt acgtttctta ccttctcctt tgtcgcaaga accgtcgcat
    16681 cgtggatctc caccggatcc atgtggatga ccagcagaat ccccaatttt tcaccgacct
    16741 catgttcaat gcggtcgata atatcatgcg atacctcgat atcgacatct cgcggcacct
    16801 ccgcatggat cgatgccata cttctgccag gcccgtagtt gtggacaatc agatcatggg
    16861 ttcctacaat tccttcatag gaagcaacct ctttcatgat ccgtgcatac agatccggat
    16921 ccgcgccctg cccgatcagc ggttccaatg tatcttttgc aatggaaata ccggaccaca
    16981 taacgagtac cgccacaaca agtcctgcga ttgcgtcgat attaaccgaa aggaaatggc
    17041 agatcaatgt agaagcgatc gttgcgctcg tcgtgatcac gtcgcccatg gaatccgccg
    17101 acgttgccag catcaccttc gactgaatgc ggtttcccag ttttcggttg aaaagcgcca
    17161 tccacagctt tacaccaacc gagagcacca gaatcaaaaa cggaaccatc tgaaatgtaa
    17221 tctcttccgg attgcgaatt ttctccaggg agctctttaa aaacgtaaag ccgacttcaa
    17281 taaccagaaa cgaaacgatc agcgctgcaa tatactcgat ccgcccatgc ccgaacggat
    17341 gctctttgtc ggccggtttg tttgccatct tgacaccgac gaagctgatc acagacgatg
    17401 ccgcgtccga gaggttgttg aacgcatccg ccataatcgc cagagaatgc atcccaacac
    17461 cgatcagcag cttggcggca aacagcaata cattgcagca gattcctacc atactggata
    17521 aaataccata tctcgtccgc acatccgcat tgtcagtgtt ctcattctct tttacaaaac
//
LOCUS       DAOMUF010000024        16715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_677_length_16715_cov_5.059244, whole genome shotgun sequence.
ACCESSION   DAOMUF010000024 DAOMUF010000000
VERSION     DAOMUF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 16715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16715
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_677_length_16715_cov_5.059244"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..381)
                     /locus_tag="PLN77_02830"
     tRNA            complement(294..381)
                     /locus_tag="PLN77_02830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Ser,seq:gct)
     gene            complement(583..1287)
                     /locus_tag="PLN77_02835"
     CDS             complement(583..1287)
                     /locus_tag="PLN77_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRM43249.1"
                     /translation="MKRILLMVLRNLLLVPWMWCRLCYHAAHPDKYSLEEHFKMLRFI
                     VQRANKGGNVIVESYGAENLPEQDGFVLYPNHQGLYDVLAIAGACSRTFTVVAKKEVE
                     HIPFLKQVFACIHALFMDRENLRQSMQVINEAADVVKEGKNVILFPEGTRSRKGNQLL
                     EFKSGSFKIAVKAKCPIVPVALKNCFVPFDSNTVRPVTVEVHFLDPIPYEEYQGMKTT
                     EIAEMVKKRIEAVVTA"
     gene            complement(1362..3869)
                     /gene="gyrA"
                     /locus_tag="PLN77_02840"
     CDS             complement(1362..3869)
                     /gene="gyrA"
                     /locus_tag="PLN77_02840"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRM43250.1"
                     /translation="MEDNVFDKIHEVDLKDTMEESYINYAMSVIASRALPDVRDGLKP
                     VQRRILYSMSELNNTPDKPHRKCARIVGDTMGKYHPHGDSSIYGALVNMAQDWATRYP
                     MVDGHGNFGSVDGDGAAAMRYTEARLSKISMEMLADINKDTVDFAPNFDETEKEPVVM
                     PARYPNLLVNGTSGIAVGMATNIPPHNLREVVSAIVKIIDNIIEEDRTTDLEEILGIV
                     KGPDFPTGAEILGTRGIDEAYRTGRGKIRVRAVSNIETLPNGKSEIIVTEIPYMVNKA
                     RLIENIAALVRDKKIDGITGIADHSNREGMRICIELRRDANANVILNQLYKHTQMQDT
                     FGVIMLALVNNQPKVMNLLEMLTYYLQHQEEVVTRRTKYDLNKAQERSHILEGLLKAL
                     DHIDEVIHIIRASANAAEAKKNLMERFDLSDAQAQAIVDMRLRALTGLEREKLENEYK
                     ELMARIEQLKAILADRKVLLGVIRREILTVSEKYGDDRRTSIGFDEYDLSMEDLIPRE
                     NTVITMTKLGYIKRMTVDNFRSQNRGGKGIKGMQTIEDDYIEELLMTTTHHYLMFFTN
                     TGRVYRLKAYEIPEASRTARGTAIVNLLQLQPGEKITAVIPMRKYEKGHYLFMATKKG
                     LVKKTPLEEYENVRKTGLAAIALRDDDELIEVKTTDNEKEIILVTKFGQCIRFKENDV
                     RTTGRVSMGVRGINLLDGDEVVGMQICTQGDYLLVVSENGMGKRTPMAEFTVQLRGGK
                     GVKCYKITEKTGNLIGSKAVNEDNEIMMITTEGIIIRTPCSGISVMGRITSGVKLMDV
                     DGEIKVASIAKVRDKEASEETPVSEKENETASEKTQE"
     gene            complement(3900..5831)
                     /gene="gyrB"
                     /locus_tag="PLN77_02845"
     CDS             complement(3900..5831)
                     /gene="gyrB"
                     /locus_tag="PLN77_02845"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRM43251.1"
                     /translation="MSTENMEKTEYGADQIQILEGLEAVRKRPGMYIGSTSARGLHHL
                     VYEIVDNSVDEALAGYCKNIDVTINPDNSITVIDDGRGIPVGINHKSGLPAVEVVFTV
                     LHAGGKFGGGGYKVSGGLHGVGASVVNALSNWLEVRVYHDGKVYFQRYERGKTMNKLE
                     VIDTCPIEKTGTEVRFLPDDTMFEETVYDYDVLKTRLRETAFLTKGLRIVLRDLRTDP
                     VVEKAFHYEGGIKEFVSYLNKSKTALYPDIIYCEGERDGVLVEVAMQHNDSYQENTYG
                     FVNNITTPEGGTHIVGFRNALTKTFNEYARANKLLKDSEPNLTGEDIREGMTAIVSIK
                     IEEPQFEGQTKQKLGNSEARGAVDNIVSTQLELFLEQNPAVAKTIVEKSLLSQRAREA
                     ARKARDLTRRKSALDGMSLPGKLADCTDKDPKNCEIFLVEGDSAGGSAKSARSRATQA
                     ILPLRGKILNVEKARLDKIYANAEIKAMITAFGTGIHDDFDITKLRYDKIIIMTDADV
                     DGAHIDTLMLTFLYRFMPELIKQGHVYLAQPPLFKIEKNKKVWYAYDDDQMNQILNEI
                     GRGDNKIQRYKGLGEMDAEQLWETTMDPERRVLRRVVFDEEYSSEIDLTFNTLMGDKV
                     EPRRDFIEERAKYANNLDI"
     gene            complement(5832..6929)
                     /gene="recF"
                     /locus_tag="PLN77_02850"
     CDS             complement(5832..6929)
                     /gene="recF"
                     /locus_tag="PLN77_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRM43252.1"
                     /translation="MYIKSLELKNYRNYESLCINISPGTNILYGDNAQGKTNILEALY
                     LAGTTKSHRGSKDREIIQFDREEAHIRMMVERNGSTHKIDMHLKKNKSKGIAIDGVPI
                     RKASELFGIVNIVFFSPEDLNIIKNGPAERRRFLDSEICQLSRLYMIELANYNKVVAQ
                     RNKLLKEISFSGRMADTLEIWDEQMVRYGTSIISERKKFISRLNDILSEIHQNLTGGK
                     EQILITYEPNVSEEAFSEELKAGRERDLRFGQSYTGPHRDDFCVRVNDIDIRKYGSQG
                     QQRTAALSLKLAEIRLVEEEIHDTPVLLLDDVLSELDGSRQNYLLQSIHSIQTLITCT
                     GLDEFVENHFEANSVFQVVEGSVFHKETGGI"
     gene            complement(6970..7179)
                     /locus_tag="PLN77_02855"
     CDS             complement(6970..7179)
                     /locus_tag="PLN77_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRM43253.1"
                     /translation="MEIITLREDYIKLGQALKAAALVEDGVEAKLVIKDGCVTVNGEV
                     DTRRGRKLYDGDVVCYNGNEIQIKK"
     gene            complement(7302..8411)
                     /gene="dnaN"
                     /locus_tag="PLN77_02860"
     CDS             complement(7302..8411)
                     /gene="dnaN"
                     /locus_tag="PLN77_02860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRM43254.1"
                     /translation="MKLVFDKADLVKSVGIAMKAVSGKTTMPILECILIDASSNVIKF
                     TSNDMELGIETIVTGMILERGIVALDAKIFSEIVRKLPDNQVTIETDENLVTTITCEK
                     AKFNIPGQSGEDFSYLPILETSDCVSISQFTLKELIRQTIFSIAANENNKLMTGELFE
                     IKDNTLRVVSLDGHRIAIRRIELKDSYPDRKVVVPGKTLNEISKILSGEMDDQIDIYF
                     SENHIMFKFDDTTVVSRLIDGEYFRIDQMLSSDYETKVTINKKEFLSCIDRATLLVKE
                     GDKKPIIIDIQDGAMELQINSAMGSMKEEIDINKEGKDIMIGFNPKFLIDALRVIDDE
                     EISIYLVNPKAPCFIRNEEESYIYLILPVNFNSVR"
     gene            complement(8754..10094)
                     /gene="dnaA"
                     /locus_tag="PLN77_02865"
     CDS             complement(8754..10094)
                     /gene="dnaA"
                     /locus_tag="PLN77_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRM43255.1"
                     /translation="MEIVQDKWKEILQRVRDEHELSEISFTTWIEPLTIHSVEDNLIT
                     ILVPSEKMGLDYVSKKYTLPIKVAIAEITGVNYEIRFILPEEVKQEKAPAPVQKNLYV
                     PNLNPKYTFDTFVVGSNNKFAHAASLAVAESPGEIYNPLFLYGGVGLGKTHLMHSIAH
                     FILQKNPSSRILYVTSEEFTNEVIEAIRNSNNTAMTKFREKYRNIDVLLIDDVQFIIG
                     KESTQEEFFHTFNTLHGANKQIILSSDRPPKDMDILEDRIRSRFEWGLLADIQSPDYE
                     TRIAILRKKQELDGYSVSDDVIEYIASNIKSNIRELEGALNKIIAYANLEKREINIDL
                     AEQVLRDIISPNEKKVITPEFIIDTVADHFDITPSDIVGSKRSSKIVFPRQIAMYLCR
                     EMLDAPLTGIGKMMGDRDHTTVMHGIEKIEKEMSAKDSVRNTVDILKKKINPSK"
     gene            10802..10936
                     /gene="rpmH"
                     /locus_tag="PLN77_02870"
     CDS             10802..10936
                     /gene="rpmH"
                     /locus_tag="PLN77_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRM43256.1"
                     /translation="MKMTFQPKKRSRAKVHGFRARMSTKGGRKVLAARRLKGRKKLSA
                     "
     gene            11035..11382
                     /gene="rnpA"
                     /locus_tag="PLN77_02875"
     CDS             11035..11382
                     /gene="rnpA"
                     /locus_tag="PLN77_02875"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRM43257.1"
                     /translation="MKYSESLKKNRDFQLVYKQGTSFANRFLVMYVKKNQLGRNRIGI
                     SVSKKVGNSVVRHHLARLIRESYRLHEEEFQCGMDVVVIARVNAKNCTYFEIEGALLH
                     LGKLHHILKETEK"
     gene            11379..11588
                     /gene="yidD"
                     /locus_tag="PLN77_02880"
     CDS             11379..11588
                     /gene="yidD"
                     /locus_tag="PLN77_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRM43258.1"
                     /translation="MKTVLIKGIRFYQKYLSPLKRVHCPYTPTCSQYGLEAIQKYGAF
                     KGSLLALWRILRCNPFSHGGYDPVP"
     gene            11612..12907
                     /gene="yidC"
                     /locus_tag="PLN77_02885"
     CDS             11612..12907
                     /gene="yidC"
                     /locus_tag="PLN77_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRM43259.1"
                     /translation="MDIVLSKSTWPIIGWVCQILGWLINGIYFCLEKIGIPNIGIAII
                     LYTIIIYLVLTPLQIKQQKMSKMMSVMQPDLQRIEKKYQNKKDQASQMKKSEETMAVY
                     QKYGVSPTGSCSTLLVQMPILLALYQVIYHIPGYIGSVRNVFQGLTTQMMGVSGYSDI
                     LTQFITDNRVTMYSKVSETLTENNLLDMFYVLKPSQWTKLADISEFSGIADTITKTAA
                     ESKHINMFLGFNITQSPMDVIREGMANGSALLIAGAILVPVLAWFTQWLNYKLMPQAP
                     SSNNGNKTANSMENSMKTMNTVMPVFSAFMCLSFSIGIGIYWIAGALVRSVQQVVINR
                     HMSSIDMDEVIRKNQEKAAKKREKAGLPPQKITQAATQSVRNIQVQENEEPSAEEKEA
                     ARKASTEYYNSNQEYRAGSIAAKANMVKQFDEKNSKKKK"
     gene            12921..13745
                     /gene="jag"
                     /locus_tag="PLN77_02890"
     CDS             12921..13745
                     /gene="jag"
                     /locus_tag="PLN77_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRM43260.1"
                     /translation="MEFIEVSAKTVDDAITKACIELQAASDKLEIQVISEGSSGFFGI
                     GSKPAVIRAAVKAAPEKEPEAPQKPEKKEAAPAPVKEAPKKEAVKKETERKSAPRQAE
                     TARRSEPREEREVVQRTDEEIAQIKATALEFLSGVFKAMELPVEIQMEYNAENGSLEV
                     DFAGEDMGILIGKRGQTLDSLQYLTSLVVNKEQKDYVRVKLDTENYRKRRKETLENLA
                     RNIAYKVKKTRRPVSLEPMNPYERRIIHSALQGNKFVETYSEGSEPYRHVVVAPKR"
     gene            13917..15305
                     /gene="mnmE"
                     /locus_tag="PLN77_02895"
     CDS             13917..15305
                     /gene="mnmE"
                     /locus_tag="PLN77_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRM43261.1"
                     /translation="MAGFHLDDTIAAVSTAMAPAGIGIVRISGNDAFEVADRVFRAKK
                     EGKKLSAVKSHTIHYGWITEGEEVIDEVLVMVMKGPKTYTGENTVEIDCHGGVLAVKK
                     VLEAVLNAGARAADPGEFTKRAFLNGRMDLTQAEAVMDVISAKSAYALKSSESQLRGT
                     VKKAIRAIREKLIYEIAFIESALDDPEHISLDGYPERLAVVVAEQKKQVEKLLASADE
                     GKMIQEGIKTVILGKPNAGKSSLLNLLAGEEKAIVTDIAGTTRDVLEENLVLKGISLR
                     ILDTAGIRKTADTVEKIGVDRALEHAKDADLILYVVDASVPLDENDAKIMEILKGRKA
                     IVLLNKSDLTAVIEKEEMEQKTGAPVISISAREETGMEELEEQMKKMFFQGEISFNDE
                     VYITNARHKQALLEAKKSLELTAGSIEMGMPEDFFSIDLMNAYEELGSIIGEAVGEDL
                     VNEIFSKFCTGK"
     gene            15350..>16715
                     /gene="mnmG"
                     /locus_tag="PLN77_02900"
     CDS             15350..>16715
                     /gene="mnmG"
                     /locus_tag="PLN77_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRM43262.1"
                     /translation="MAVLEETIDIAVIGAGHAGCEAALAAARMGLETVVFTVSVDSIA
                     MMPCNPNIGGTSKGHLVKEIDALGGEMGKNIDKTFIQSKMLNKSKGPAVHSLRAQADK
                     RAYSQSMREVLENTDHLTIRQMEIAELIVEDGVLTGVKAVSGAIYHCKAAVLCTGVYL
                     NARCIYGDVSTYTGPNGLQAATHLTDSLKANGVEMVRFKTGTPARIDKRSIDFSKMEE
                     QFGDERVVPFSFSTDPESVQIDQESCWLTYTNEETHKIIRENLSRSPLYSGMIEGTGP
                     RYCPSIEDKVVKFADKNRHQVFLEPEGRYTNEMYVGGMSSSLPEDVQIAMYHTVPGLE
                     HAKIVRNAYAIEYDCINPRQLLPSLEFKAIKNLFSGGQFNGSSGYEEAAAQGLIAGIN
                     AALRVQGKEELVLDRSESYIGVLIDDLVTKENHEPYRMMTSRAEYRLLLRQDNADLRL
                     RKYGY"
BASE COUNT         4226 a         3896 c         4118 g         4475 t
ORIGIN      
        1 tcggatcagc tcatttattt tatcacagaa cttttcgttt gtcaagaact ttttttaaat
       61 tatttttcga agttcttttg aacttcccgg gaacgttttg ttccctgctg ttctctcgat
      121 cagcaactcg tttattttat caaactcatt cgcttttgtc aagaactttt ttaatttatt
      181 ttttgggatt agttttttca aacccatcgc caactctgct atcttatcag atgtgaagct
      241 gtttgtcaag aactttttcc acaaaaaata tcattttgtt tttggctttt aaacggagaa
      301 ggagggattt gaaccctcgc gccggttacc cgacctacac ccttagcagg ggcgcctctt
      361 cagccacttg agtacttctc caaatgccta aatataaaat gatatgtgcg gtcactcatt
      421 cgtgacgcat ggtttattat acaaaaacct tacgttcttg tcaacccgtt ttttcgattt
      481 ttttgttttt ttcttactat tatagaaaat atgccttaca gaacccaaat cagcccccag
      541 aatagcagaa aagagcgcat atcacgctct tttctgaaac cgctacgcgg ttacgacggc
      601 ttcaattctc ttctttacca tctcggcaat ctctgttgtc ttcattccct ggtattcctc
      661 gtatgggatc gggtcgagaa aatggacttc taccgttacg ggacggacgg tattggagtc
      721 aaacggaaca aaacagtttt ttaacgcaac cggcacgatc gggcattttg cttttactgc
      781 gatcttgaag ctgccgcttt taaactccag cagctggttt ccctttctgc tcctcgttcc
      841 ttccggaaac agaatgacgt ttttgccttc ttttacgaca tcggccgcct cattgataac
      901 ctgcattgac tgccgcagat tctcccggtc cataaacagc gcgtggatgc acgcaaagac
      961 ctgttttaaa aacggaatat gctctacctc ttttttggca acaactgtaa aggtacgcga
     1021 gcacgctcct gcgatcgcca gcacatcgta cagtccctgg tgattcggat acagcacaaa
     1081 accgtcctgc tccggaagat tttctgctcc ataggattcc acaatgacgt ttccgccttt
     1141 gttcgcccgc tgtacaataa agcgcagcat tttaaagtgc tcttccagac tgtacttgtc
     1201 cggatgcgct gcatggtaac agagccggca ccacatccac ggaaccagaa gcagattccg
     1261 cagcaccatc agtaaaattc gtttcatgtt tttctcccct tccccaaacg agcaaaacgt
     1321 gcctgagctt ttttctctca ggcacgtttt gcgacgctgt attattcctg cgttttctca
     1381 gaagccgttt cgttctcttt ctccgaaacc ggagtctcct cggacgcctc tttgtctctt
     1441 acttttgcaa tgctggcaac cttgatttcg ccgtccacat ccatcagctt aacgccggat
     1501 gtgatccgtc ccatcacaga aatgccggag catggcgtac ggatgatgat tccctctgtg
     1561 gtgatcatca tgatctcatt atcctcgttg accgccttgg aaccgatcag gtttccggtc
     1621 ttttcggtaa tcttatagca tttgacaccc ttgccgccgc ggagctgtac ggtaaactct
     1681 gccatcggcg tccgtttgcc catgccgttt tcggatacga caaggagata gtctccctgc
     1741 gtgcagatct gcatgccgac tacttcatcg ccgtccagaa ggttgatgcc gcgtacaccc
     1801 atggaaacac ggcctgtcgt acgcacatcg ttttccttga agcggataca ctggccgaat
     1861 ttcgttacca gaataatctc tttctcgtta tcagttgtct taacttcaat cagttcatca
     1921 tcatcgcgga gcgcaatggc agcaagaccc gtttttctga cgttctcgta ttcttcgagc
     1981 ggcgtcttct tcaccagtcc ctttttcgtt gccataaaca gataatggcc cttctcatat
     2041 ttgcgcattg gaataacagc ggtaatcttc tctcccggct gcagctggag aaggttgaca
     2101 atcgccgttc ctcttgcggt tctggacgcc tccggaatct cgtatgcttt cagacggtag
     2161 actcttcccg tattcgtaaa gaacatcagg taatgatggg tcgtcgtcat cagaagctcc
     2221 tcgatgtagt cgtcctcgat ggtctgcatg cccttgattc ccttgccgcc gcggttctgg
     2281 ctgcggaagt tgtcaacggt catacgtttg atatagccca gtttcgtcat ggtgatgacg
     2341 gtattctcgc gcggaatcag atcctccatg gagagatcgt actcatcaaa gccgatggaa
     2401 gttcttctgt catcgccgta tttttcggat acggtcagaa tctccctgcg gataacgcca
     2461 agaagcacct tgcggtctgc gaggatggct tttaactgct cgatgcgcgc catcagctct
     2521 ttgtactcgt tctcgagctt ctcccgttcc agaccggtca gagcgcgcag acgcatgtct
     2581 acgattgcct gggcctgggc atcactcaga tcgaagcgtt ccatcaggtt cttcttcgcc
     2641 tctgccgcat tcgcggatgc acggatgata tggatcactt cgtcaatatg gtcaagcgct
     2701 tttaagagtc cttccagaat atggcttctc tcctgcgctt tgttcaggtc gtatttcgtt
     2761 cttctcgtca caacctcttc ctggtgctgc agataatacg tcagcatctc cagaaggttc
     2821 atgactttcg gctgattgtt taccagtgca agcatgatca caccaaaggt atcctgcatc
     2881 tgggtatgtt tgtacagctg gtttaagatg acgtttgcat tggcgtcgcg gcgcagttcg
     2941 atacagatgc gcataccctc acggttggag tggtccgcaa tgccggtgat gccgtcgatc
     3001 tttttatctc ttaccagcgc ggcgatgttc tcgatcaggc gcgccttgtt taccatgtac
     3061 gggatctcgg ttacgatgat ctcgctcttt ccgttcggca gtgtctcgat gtttgatacc
     3121 gcacgaacac ggattttacc gcggccggta cggtaggcct cgtcgatgcc gcgggtgccg
     3181 aggatttctg ctccggtcgg gaagtcaggg ccttttacaa tgccgagaat ctcctcgaga
     3241 tctgtcgtac ggtcctcctc gatgatatta tcaataattt ttacaatggc acttaccacc
     3301 tcgcgcagat tgtgcggcgg gatgttcgtt gccataccga ctgcgatacc ggaagtaccg
     3361 tttaccagaa ggttcgggta gcgggccggc ataacaacag gctctttttc ggtttcatca
     3421 aagttcgggg cgaagtctac ggtgtctttg ttgatatccg ccagcatctc catcgagatt
     3481 ttgcttaaac gtgcctcggt gtatcgcata gccgcagcgc cgtcgccgtc gacggaaccg
     3541 aagtttccat ggccgtccac catcggataa cgggttgccc agtcctgtgc catgttgacg
     3601 agtgctccgt agatagagct gtcgccgtgc gggtgatatt tacccatcgt atcaccgacg
     3661 atacgcgcgc acttacggtg cggcttatcc ggcgtattgt tcagttcact catggagtac
     3721 agaatacgtc tctgtaccgg ctttaagcca tcgcggacgt ctggaagtgc acgggatgca
     3781 attacgctca tcgcgtagtt gatgtaggat tcctccatcg tgtctttcag atcgacctcg
     3841 tgaattttat caaaaacatt atcttccatc ttttcctcca cttcagttca caggactttt
     3901 tagatatcca ggttgtttgc gtattttgct ctctcttcga taaagtcacg gcgcggttct
     3961 accttgtcgc ccatgagcgt attgaaggta agatcaattt cagaagaata ctcttcatcg
     4021 aacacaacgc ggcgcagaac acggcgttcc gggtccatgg tggtctccca gagctgctcc
     4081 gcatccatct ctcccagtcc tttgtaacgc tggatcttat tatctccgcg tccgatctca
     4141 ttgaggatct gattcatctg atcgtcgtcg tatgcgtacc atactttttt gtttttctca
     4201 atcttgaaca gcggcggctg tgccaggtaa acgtggccct gtttgatcag ctccggcatg
     4261 aagcggtaca ggaacgtcag catcagtgtg tcgatgtgcg cgccgtcgac atcggcatcg
     4321 gtcatgatga tgatcttgtc gtagcgcagt ttggtgatat cgaaatcgtc gtggattccg
     4381 gttccgaatg ccgtaatcat cgctttgatc tcggcattgg cataaatttt atcgagacgt
     4441 gctttctcta cattcaggat ttttccacgc agaggcagga tcgcctgcgt tgcacggctt
     4501 ctcgcggatt ttgcggaacc gcccgcggaa tctccctcga ccaggaagat ctcacagttt
     4561 tttggatcct tgtcggtgca gtctgccagt tttccaggca gggacatacc gtccagtgcg
     4621 gattttcttc tcgtcaggtc acgcgctttt cttgcagcct ctctcgcacg ctgggaaagc
     4681 agggactttt caacgatcgt ttttgcgaca gccgggttct gctccaggaa cagctcgagc
     4741 tgggtgctca cgatattgtc gactgctcct ctcgcctcgc tgtttccaag tttctgcttt
     4801 gtctggccct cgaactgcgg ctcctcgatt ttgatgctga caatcgccgt cataccctcg
     4861 cggatatcct ctccggtcag gttcggttcg ctgtctttta acagtttatt tgctcttgca
     4921 tattcattaa aggttttcgt cagtgcattc cggaagccca cgatatgggt accgccctcc
     4981 ggcgtcgtaa tgttgttgac gaaaccgtac gtattttcct gataggaatc gttgtgctgc
     5041 atcgcaacct caacgagaac gccgtctctt tctccctcgc agtagatgat gtccgggtag
     5101 agcgccgttt tgctcttgtt caggtagctg acaaactctt tgataccgcc ctcgtagtgg
     5161 aatgcttttt ccactaccgg atccgtgcgc agatcgcgca gaacaatgcg cagtcccttg
     5221 gtcagaaatg cggtctcgcg gagtcttgtt tttaaaacgt cgtaatcata aaccgtctcc
     5281 tcgaacatcg tgtcatccgg caggaaacgg acctcggtac ctgttttttc gatcggacag
     5341 gtatcgatga cctccagctt attcatggtt tttccgcgct cataccgctg gaagtaaacc
     5401 tttccatcgt gataaacgcg cacctcaagc cagttggaca gcgcatttac aacggatgca
     5461 ccaacgccgt gcagaccgcc ggataccttg taccctccgc cgccgaactt tccgcctgcg
     5521 tgcagcaccg taaagacaac ctcaacggcc ggaaggcccg acttatgatt gatgccgacc
     5581 ggaataccac ggccgtcgtc gatgacggtg atggagttgt ccgggttaat cgtaacgtca
     5641 atatttttgc agtatcctgc cagtgcctcg tctaccgagt tgtcgacaat ctcatagacc
     5701 agatggtgaa gtccccgtgc ggaggtactg ccgatataca tacctgggcg cttccgtacg
     5761 gcttccagac cctccagaat ctgaatctgg tctgcaccat actctgtctt ttccatgttc
     5821 tctgtactca tttagattcc tcctgtttct ttgtgaaaca cagatccctc gaccacctgg
     5881 aatacgctgt ttgcctcaaa atgattttcg acaaactcgt ccagtccggt acaggtaatc
     5941 agtgtctgta tgctgtgaat gctctgcaga agataattct gccggctgcc gtccagctcc
     6001 gacaaaacat catccagcag taaaaccggt gtatcgtgaa tttcctcctc gaccagacgg
     6061 atctccgcca gtttcagcga aagcgccgcc gtcctctgct gcccctggga accgtattta
     6121 cggatatcaa tgtcgttgac ccgcacgcag aaatcgtccc ggtgcggccc ggtgtaggac
     6181 tgtccgaaac gcagatcacg ctctcttccg gcttttaact cttcggaaaa cgcttcctcg
     6241 gacacattcg gttcataagt aatcagaatc tgctcctttc ctccggtcag attctgatgg
     6301 atctcggata aaatatcatt caggcggctg ataaactttt tccgctcgga aataatgctt
     6361 gttccatagc ggaccatctg ctcatcccag atctcgagcg tatccgccat tctgccggaa
     6421 aacgaaatct ctttaagcag cttgtttctc tgcgccacca ctttattata attggcaagc
     6481 tcgatcatgt atagtcttga caattgacaa atttcgctat ccaggaaacg ccggcgctcc
     6541 gcaggaccgt ttttgatgat attcaggtcc tccggagaga aaaacacaat atttacaatg
     6601 ccaaaaagtt ccgacgcttt gcggatcgga actccatcga tggcgattcc cttggattta
     6661 ttttttttca gatgcatatc gattttatgc gtgctgccgt tccgctccac catcatccgg
     6721 atatgggctt cctcccggtc aaactggatg atttcgcggt ccttgctgcc acggtgcgat
     6781 tttgtcgtac ccgcgagata taaagcctcg agaatattcg tctttccctg ggcattgtcg
     6841 ccgtagagaa tattcgttcc ggggctgata tttatgcata aactctcata attccgatag
     6901 ttcttcagtt ctaaagattt gatatacatg agcactcgtc cgtgaaaaat cagcttaagt
     6961 ttttaagcct tattttttaa tttgtatctc atttccattg tagcagacaa catctccgtc
     7021 atagagcttg cggccgcgac gggtgtctac ttcaccgttt acggtcacgc agccgtcctt
     7081 gatgacgagc tttgcttcca cgccgtcttc taccagagcc gcagctttta atgcctggcc
     7141 cagtttgatg taatcttcac gcagcgtaat aatttccatt ttttcctcct gcactgccgt
     7201 gccttttcct cacgtaagtt cgcagttttc aacctgtgtg gctgtaaaac cgctgaaagt
     7261 tctttcagaa aaggttccgg cagatttcaa acttatgtga cttatctcac ggaattgaag
     7321 tttaccggca gaatcagata gatatagctc tcttcttcgt tccggataaa acatggagcc
     7381 ttcgggttga ccagataaat cgaaatttcc tcgtcgtcaa taacacgaag tgcatcgatc
     7441 aggaatttcg ggttaaaacc gatcataata tccttgcctt ctttgttgat atcgatctct
     7501 tctttcatgg aacccatcgc ggagttgatc tgcagttcca tcgctccatc ctggatgtcg
     7561 atgatgatcg gttttttgtc gccttctttg acaagaagcg tcgcacggtc gatacagctt
     7621 aagaattctt ttttgttgat cgtcactttt gtctcgtaat cactcgaaag catctggtcg
     7681 atgcggaaat attcaccgtc gatcagtctg gaaacgaccg ttgtatcatc gaacttgaac
     7741 ataatgtggt tttccgagaa gtaaatatca atctgatcat ccatctcgcc ggacagaatt
     7801 ttgctgattt cgttcagggt ttttcccgga acaacgactt ttcggtccgg atacgaatct
     7861 ttcagctcaa ttctgcggat cgcaatacgg tgtccgtcca gagatacgac gcgaagtgtg
     7921 ttatctttga tttcaaaaag ctcaccggtc atcagtttgt tgttctcatt ggctgcaatg
     7981 gagaaaatgg tctgacggat cagttctttt aaggtgaact gggaaatgga aacacaatcg
     8041 gaagtttcga gaatcggcag ataggaaaaa tcttctcctg actgtcccgg aatgttgaat
     8101 tttgcttttt cacacgtaat cgtggttacc agattttcat ctgtttctat tgtaacctga
     8161 ttatccggaa gttttcttac gatctcagaa aaaatctttg catccagagc aacaataccg
     8221 cgttccagaa tcatacctgt gacgatggtt tcgattccga gttccatatc gttggaggta
     8281 aatttgatca cgttggaaga tgcatcaatc agaatgcatt ccagaatcgg catggttgtt
     8341 ttaccggaga cagctttcat agcgatacca acgcttttta caaggtctgc tttgtcaaac
     8401 actaatttca taattgtgga taactctcct ttcctcactg tttggtgagt ctgcttataa
     8461 agaaaaaata tagtaaaaat ccgtagtctt agtcgtaggg gctgtggata tgtggaaaac
     8521 cccgtcaaga atgcggaaat actggaaaaa tcgatctgta taactctgtg aataatcgaa
     8581 cgtgaacctg tgtatgaaat gttgataact tcaggcccca aatttgtcca caattcatcc
     8641 acaggctttc cacatcggtt tgtgtataaa taagggcctg tcaaccgatg agtttcttct
     8701 tatataaatg taaatcggtt ttaaccgttt tgacagagtt acaaaggctc cgcctatttc
     8761 gacgggttga tcttcttctt taagatgtct accgtgttgc ggacactgtc tttagcactc
     8821 atttcctttt cgatcttctc aatgccgtgc atgacagtgg tatgatcgcg gtcgcccatc
     8881 atttttccga ttccggtcag cggggcatcg agcatttcgc ggcacagata cattgcgatc
     8941 tggcgcggga atacgatttt gctgctccgc ttgctgccga caatgtcgga cggcgtaata
     9001 tcgaagtgat ctgcaacggt gtcgatgata aattccggcg tgatcacctt cttctcgttc
     9061 ggggagatga tgtcgcgcag cacctgctct gcaagatcaa tgttgatttc acgtttttca
     9121 aggtttgcgt atgcaatgat tttattgaga gcgccttcca attcacggat gttcgattta
     9181 atattggaag cgatgtattc gatcacatcg tcgctgacgg aatagccgtc taattcctgt
     9241 ttctttcgga gaatggcgat tcgcgtctcg taatccgggc tctggatgtc ggcaaggagt
     9301 ccccattcga aacgggaacg gatgcggtct tccaggatat ccatgtcttt cgggggacga
     9361 tccgaagaaa ggatgatctg tttgttggcg ccgtgcagcg tattgaaggt atggaaaaac
     9421 tcttcctgcg tggattcctt tcctataata aactgcacat catcgatgag aagtacgtcg
     9481 atattgcggt atttctcacg gaatttggtc attgcggtgt tgttgctgtt tcggatggcc
     9541 tcgatgactt cattggtaaa ttcttcactc gttacgtaga gaatccgcga agacgggttc
     9601 ttctgaagaa taaaatgtgc gatcgagtgc atcagatggg ttttgcccag tccaacacca
     9661 ccgtataaaa acagcggatt gtagatttct cccggggatt cggccacggc gagagaagct
     9721 gcatgggcaa atttgttatt gctgccgacc acgaaggtat caaaggtata ctttggattc
     9781 aggttcggca catagagatt tttctggacg ggagcaggcg ctttttcctg tttcacttct
     9841 tccggaagaa taaagcggat ctcatagttg acacctgtga tttccgcgat ggccacttta
     9901 ataggaagag tatatttttt gctcacgtaa tcgagaccca ttttttcgga tggaaccagg
     9961 attgtgatca gattgtcctc aaccgaatgg atcgtaagtg gttcaatcca tgtagtaaag
    10021 gagatctcag ataattcgtg ctcgtcccgc actctctgca gtatttcttt ccatttatct
    10081 tgtacgattt ccatgctttt tctgtcgctc cgcttctata atcattgctt tttggggata
    10141 acttctgtct ggaaaacgaa attttccccg aatagtcgca tttttacttc tatatgatat
    10201 aataaaatgc cgttttattc aacccaaaaa tgtggataat tattttttta tccacagaat
    10261 gtgtaaaaaa ttttcctgtt gatgaaatgg aggatagttt gtggataaca gggaatctta
    10321 tgttgataaa attgtggata gagggctgga aaaaccgaaa tgtgtaaagt ctggaaaact
    10381 tatatacaca ttatacatgg cgtaaatact ggaaaaattg gaaatgccca caggttgtac
    10441 acagcaaaac cgaaagttat ccacaaggtt atccacagat tgtggataat gttgattacg
    10501 gttacaagat atcgtgtgga aagcgcaaaa atcgaactgg tatttgtacg cgtgcggcgg
    10561 acagtgtgga taagttgtgg ataaaccgaa aatcagcaca cggaaggcac gggcagcggc
    10621 agaccggaaa accgcgtcag agcggcattt ttcccgaaaa aactgcgaaa attattgatt
    10681 ttagggttga cgaaatgggg gtttatgaat ataattgaaa tgatgttttc tgtcatacat
    10741 gtagtgaaat actgccttgc aggcagttat tgattagaat ttaaaggagg tgccttatct
    10801 tatgaaaatg acatttcagc cgaaaaaaag atccagagct aaagttcacg gattcagagc
    10861 aagaatgagc acaaaaggtg gaagaaaagt tttagctgca agaagattaa aaggaagaaa
    10921 aaaattatca gcataaaacc aaaggccgca taaaatttat gtggtctttt cttctatcta
    10981 aagctattca atgaaaagaa aatttagaca aatcatgaag gaaagaatgg attcatgaaa
    11041 tattcggaaa gcctgaaaaa aaacagagat tttcagctgg tttataagca gggaacatcg
    11101 tttgcaaacc gatttctggt catgtatgtg aagaaaaatc agctgggacg gaaccggatt
    11161 ggaatttcag taagtaaaaa ggtgggaaac agcgttgtgc gccaccatct ggcgagactg
    11221 atccgggaga gttatcgtct gcatgaagag gaatttcagt gtggaatgga tgtagttgtg
    11281 atcgcaagag ttaatgcaaa aaactgtacg tatttcgaga tagagggagc acttttgcat
    11341 ctgggaaaac tacatcatat attaaaggaa acagaaaaat gaagacggta ttgataaaag
    11401 gtatccggtt ttatcagaag tacctgtctc ctctgaagag ggtgcactgc ccttatacgc
    11461 cgacctgctc gcagtacggg ctggaagcga ttcagaaata tggtgctttc aaaggaagtc
    11521 tgctggcatt atggagaata ctgagatgta acccgttttc acacggaggt tacgaccctg
    11581 tgccgtagat aaataaggag gaaaagacgt tttggatatc gtactgagta agtcaacgtg
    11641 gccgattatc gggtgggtat gccagatcct cggctggctg atcaatggaa tttatttttg
    11701 cctggaaaaa attggaattc cgaacatcgg tattgcgatt attctgtata cgattatcat
    11761 ttatctcgtt ctgacgccgc ttcagatcaa gcagcagaag atgtcgaaga tgatgagcgt
    11821 aatgcagccg gatctgcaga gaattgaaaa gaaatatcag aacaaaaaag accaggcttc
    11881 tcagatgaaa aaatctgaag aaaccatggc agtttatcag aaatacggtg tctccccgac
    11941 aggaagctgc agtacactgc tggttcagat gccgattctg cttgcactgt accaggttat
    12001 ctatcatatt ccgggatata tcggaagtgt ccgcaatgta ttccagggac tgacaacaca
    12061 gatgatgggt gtatcaggat attctgacat cctgacacag tttatcacag ataaccgtgt
    12121 taccatgtac agtaaagtaa gtgaaacatt aaccgaaaat aaccttctgg acatgtttta
    12181 tgtgctgaaa ccgtcccagt ggacgaagct ggccgatatc agcgagttct ccggtattgc
    12241 ggataccatt acaaaaaccg ccgcggagtc caagcatatc aacatgttcc ttggatttaa
    12301 tatcacacag tctccgatgg atgtcatccg cgagggtatg gcaaacggat ctgctcttct
    12361 gattgcagga gcaattctgg tgccggttct ggcatggttt acccagtggc tgaactacaa
    12421 gctgatgccg caggcaccat cttccaacaa cggaaataag acagcaaatt ccatggaaaa
    12481 ttccatgaag accatgaaca cggtaatgcc tgttttctcc gcatttatgt gtttatcgtt
    12541 ctccatcggt attggtattt actggattgc aggtgcgctt gttcgaagcg tacagcaggt
    12601 tgtgatcaac cgtcatatgt cctcgattga catggatgag gtgatccgca aaaatcagga
    12661 aaaagcggcc aagaagagag aaaaagccgg ccttccgccg cagaaaatca cacaggctgc
    12721 aacgcagagt gtgcgtaata ttcaggtaca ggagaacgaa gagccgtctg ctgaggaaaa
    12781 agaggcagca agaaaggctt cgactgagta ttacaacagt aatcaggaat accgcgccgg
    12841 aagcatcgct gcgaaagcaa acatggtaaa acagtttgat gaaaagaaca gcaaaaagaa
    12901 aaaatagggg gtaaatcttt atggagttta tcgaagtatc cgcaaaaaca gtggacgacg
    12961 ctattacaaa agcctgcatt gagcttcagg ctgccagcga taaactggag atccaggtca
    13021 tcagcgaagg cagttccgga ttctttggaa tcggaagtaa accggctgtg attcgtgccg
    13081 ctgtaaaagc cgcaccggaa aaagaaccgg aagcacccca gaaaccagag aaaaaagagg
    13141 cagctcctgc accggtaaag gaagcgccga aaaaagaagc tgtaaagaaa gaaacggaaa
    13201 gaaaatctgc tccgcgtcag gcagaaactg caagacgctc cgagccaaga gaagagcgtg
    13261 aagtagttca gagaacagac gaggaaatcg cccagatcaa ggcaacggca cttgagttct
    13321 taagcggtgt attcaaagcc atggagctcc cggttgagat ccagatggag tataatgccg
    13381 agaacggttc tcttgaagtt gattttgccg gtgaggatat gggtatcctg atcggaaaaa
    13441 gaggacagac actggattcc cttcagtatc tgaccagcct tgtggtcaac aaagagcaga
    13501 aggattacgt tcgtgtcaaa cttgatacgg agaactaccg caaacgcaga aaagaaacac
    13561 tggaaaatct ggcaagaaac atcgcttaca aagtgaaaaa gaccagaaga ccggtttctc
    13621 tggagccaat gaatccttac gagagaagaa tcatccactc tgcactgcag ggaaataaat
    13681 tcgtggagac ctacagcgag ggcagcgagc cataccgcca cgtagttgta gcgccgaaac
    13741 gataagaaag cgtaaaaaat atgcaaaaag acggtacctg ttgaaaatac ggggtaccgt
    13801 cttttctggt tttctgctaa aatggaacta ttcagagcca tgcgaaagga aagaaaaaat
    13861 gtggatctga atactgggat ccccaaaaag agaggtttta gaaaggactg gaaacaatgg
    13921 caggatttca tttggatgat acaattgccg cagtgtccac agcgatggcg ccggcgggaa
    13981 ttggtattgt aagaatcagc ggaaacgatg cgttcgaagt ggcggaccgc gtattccgtg
    14041 caaaaaaaga aggaaaaaag ctttccgcgg tcaaatccca tacgattcat tatggatgga
    14101 ttaccgaagg ggaggaagtg attgatgagg tccttgtcat ggtgatgaaa gggccgaaaa
    14161 catatacggg cgagaatacc gtcgagatcg actgtcacgg cggggtgctg gcggtgaaaa
    14221 aagtgctcga ggcagtctta aatgccggtg cgcgggcggc agatccgggt gagtttacga
    14281 aacgggcgtt tctgaacgga agaatggatc tgacgcaggc cgaggcggtt atggatgtca
    14341 tctcggcaaa gagtgcgtat gccttaaaaa gctccgagag ccagctgcgc ggaacggtga
    14401 aaaaggcgat ccgggcaatt cgcgagaagc tgatttatga gatcgcgttt atcgagtcgg
    14461 cgcttgatga cccggagcac atcagtctgg acggataccc ggagcggctg gccgtggttg
    14521 ttgcggagca gaaaaagcag gttgaaaagc tcctggcatc tgcggatgag ggtaagatga
    14581 tccaggaggg catcaagaca gttattcttg gaaagccgaa tgccggaaaa tcgtcgcttt
    14641 tgaatctgct ggccggagag gaaaaggcga ttgtaacgga tatcgccgga acgacgcgcg
    14701 atgtgctcga ggaaaatctg gtgctgaagg gtatttccct tcggattctc gatacggcgg
    14761 gtatccgcaa gacggcggac acggtcgaga aaatcggcgt ggaccgtgcg ctcgaacacg
    14821 caaaagatgc ggatctgatc ctctacgtgg tggatgcgtc ggtgccgctc gatgagaatg
    14881 atgcgaaaat tatggagatc ctgaaagggc gcaaggcgat tgtgctgctg aataaatcag
    14941 atctgaccgc ggtgatcgaa aaagaggaga tggagcagaa gacaggtgct ccggtcatca
    15001 gcatttcggc gcgggaagag acgggcatgg aagagctgga agagcagatg aaaaagatgt
    15061 tcttccaagg tgaaatttcg ttcaacgatg aggtttacat cacgaatgcg cgccataaac
    15121 aggcgcttct ggaagcaaaa aagagtctgg agctgacggc aggcagcatc gaaatgggta
    15181 tgccggagga cttcttctcg attgacctaa tgaatgcgta tgaggaactt ggctcgatca
    15241 tcggtgaagc ggtaggagag gaccttgtga atgagatttt cagcaagttc tgtacaggca
    15301 aataatcacg aaacagttcg tagagtttag atagaaaagg acaggaaaaa tggcagtttt
    15361 agaagaaaca attgatattg ccgtcatcgg tgcgggccat gcgggatgcg aggcagcact
    15421 tgcggcagcg cggatggggc tggaaacggt tgtatttacg gtaagtgtcg acagcatcgc
    15481 gatgatgccg tgcaatccga acatcggagg aacctcgaag ggacacctcg taaaagagat
    15541 cgacgcgctc ggtggcgaga tgggtaaaaa tattgataag acgtttattc agtcgaaaat
    15601 gctgaataaa tcaaagggac cggcggtgca ttcgctgcgt gcgcaggcag acaagcgggc
    15661 gtacagccag tcgatgcgcg aggttctcga aaacaccgat catctgacga tccgccagat
    15721 ggagattgcg gagctgatcg tggaagacgg tgttctgacg ggcgtaaaag ccgtttccgg
    15781 tgcaatttac cactgcaagg ctgcagtgct ctgtaccggt gtttacctga atgcgcggtg
    15841 catttacggt gatgtgagca cgtatacggg accgaacggt ctgcaggcgg caacgcatct
    15901 gacggattcc ctgaaagcaa acggcgtgga gatggtgcgc tttaagaccg gaacaccggc
    15961 gcgtatcgac aagcggagca tcgacttctc caagatggaa gagcagttcg gcgatgagcg
    16021 ggttgtgccg ttctcgttct cgaccgatcc ggagagtgta cagatcgatc aggaatcctg
    16081 ctggctgacg tatacgaacg aggagacgca caaaatcatc cgcgaaaatc tcagccggtc
    16141 gccgctctat tccggcatga tcgagggaac tggtccgcgg tactgtcctt cgattgagga
    16201 taaagtcgta aaatttgccg ataaaaaccg tcatcaggtg ttcctggagc cggagggaag
    16261 atacacgaat gagatgtacg tcggcggcat gtcgagttca ctgccggagg atgtgcagat
    16321 tgcgatgtat cacaccgtgc cgggtctgga gcatgcaaaa attgtgcgga atgcgtatgc
    16381 gatcgagtac gactgcatca acccgagaca gcttctgccg tccctggagt tcaaggcgat
    16441 caaaaatctg ttttccggcg gccagtttaa cggcagttca ggatacgagg aagctgcggc
    16501 acagggcctg atcgcgggca tcaatgcggc gctccgcgtg cagggaaaag aggagctggt
    16561 gctcgaccgt tccgagtcgt acatcggtgt tctgatcgat gatctcgtaa cgaaagagaa
    16621 ccacgagccg taccgaatga tgacgagccg cgcggaatat cgtctgctgc tccgccagga
    16681 caatgcggac ctgcgtctgc gcaaatacgg atacc
//
LOCUS       DAOMUF010000025        16226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_725_length_16226_cov_4.734648, whole genome shotgun sequence.
ACCESSION   DAOMUF010000025 DAOMUF010000000
VERSION     DAOMUF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 16226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16226
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_725_length_16226_cov_4.734648"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1591
                     /locus_tag="PLN77_02905"
     CDS             62..1591
                     /locus_tag="PLN77_02905"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HRM43263.1"
                     /translation="MQKITFRKLAGEKYIYPELQGHFIEFLGSCIYDGIWVGEDSEIP
                     NYHGIRKDLVDAFQKLHPPVIRWPGGCFADVYHWRNGIGPREQRPVTYNENFGTFETD
                     PNQFGTHEMMEFCEMIGAKPWFNINMMTGSPSEMREWMEYCNRKEPTALSQERKTNGH
                     EKPFEIEYWGIGNEVWDGGGKMTPQMYANEYRKFSSSCPSFGGGDQAFSMKCIASGPD
                     GNKPKERAAWTKDFFKEMGKYRMPSLYGYDLHFYNWNLKQLQTEKKFDEKQWYDVING
                     CKELENVIHEQRRLIDAGLEALPKPEGPFQAAPRKCELIVGEWGNWHSSAFNARPALY
                     QQCTMRDAVTTALTLDIFHRNTGDVRMACVAQSVNVLNSLFLTDGEHCILTPNYDVFD
                     MYQVHQGAYTLGFEEKNKDPEVCIFASIKNDDIYVNLVNTSYSESKKIELKFKQCPEF
                     VEAKTLYSKDPQNYNDAKHPNRVRCKEGKAPAREKDSFQIALPAASVSVYHFRKTETE
                     K"
     gene            complement(1652..1957)
                     /locus_tag="PLN77_02910"
     CDS             complement(1652..1957)
                     /locus_tag="PLN77_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRM43264.1"
                     /translation="MMYNNYIYIAVMALVSYTIRILPLTLIRKPIKNQFIQSFLYYVP
                     YVTLSVMTFPAIVHATQSPVSGALALVVGIAAAWFGASLFQVSVACCAVVFVIELFL"
     gene            complement(1944..2687)
                     /locus_tag="PLN77_02915"
     CDS             complement(1944..2687)
                     /locus_tag="PLN77_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRM43265.1"
                     /translation="MSSQRSYSFSRQVLVNGIRDGIPIGLGYFAVSFSLGIAARKAGL
                     SPFQGFLASLFNNASAGEYAAFTLIAANAGYLQVAIITLIANARYLLMSCALAQRFSP
                     DTPFFHRFLIGYDVTDELFGITIARPGWLNPYYTYGAILVAAPAWSIGTALGIIAGNL
                     LPLRAVSALSVALYGMFLAIIIPPARKSRVVAGLVAVSFFLSFICSYLPGISTLSDGT
                     RTILLTVLISCAGAILFPVKTEEENADVQ"
     gene            3255..4136
                     /gene="ispE"
                     /locus_tag="PLN77_02920"
     CDS             3255..4136
                     /gene="ispE"
                     /locus_tag="PLN77_02920"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRM43266.1"
                     /translation="MKLRALAKINLGLDVTGKREDGYHEVRMVMQTIQMYDQLEIKES
                     KEPGIRLTTNLPFLPCNDGNLVYKAAKILMDEFDIRQGVDMNLTKFIPVAAGMAGGSS
                     DAAAALVGINRMFQLGLTKRQLMERGVQIGADVPYCVMRGTALAEGIGEKLTSLPGVP
                     MCYVLIGKPGINVSTKFVYGNLHLDEVTEHPDIDGMVEAIRNHDLYGITDRMGNVLES
                     VTCPAYPVIDEIKAQMMKNGAVNAMMSGSGPTVFGIFDDPDKAEFARDQLKISGLSKQ
                     TFLTTVSKPGGTKYGKK"
     gene            4123..4836
                     /locus_tag="PLN77_02925"
     CDS             4123..4836
                     /locus_tag="PLN77_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM43267.1"
                     /translation="MAKNELDLQMEDYQYLPLRDVVFRTLRQAILRGELKPGERLMEI
                     RLANQLGVSRTPIREAIRMLELDGLVIMVPRKGAQVAQITEKDLNDVLEVRLGLEELA
                     VKLACQRITESELQKLYQASRSFEQMLETTETDDLQKLAQADVAFHDVIYQATNNERL
                     IQLLNNLREQMYRYRIEYLKDVKSRRSLVEEHDALYERMKNRDLAGAQKMIREHIERQ
                     QESIMQTVHHQSMTGVEEK"
     gene            4982..6514
                     /locus_tag="PLN77_02930"
     CDS             4982..6514
                     /locus_tag="PLN77_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HRM43268.1"
                     /translation="MEKEKTQISLTLAENESVIRTWCENCDDIQIRPMKLGKTGGTGA
                     LLIYVEVAVSCVMLKDSVIGKLLNRLMEVPEADIPGVLSENQLGISDTLEFDTLEDAF
                     ASMLAGNAVLFVDGYDCAVKIGSKGYPNMGVQKAESEKVLRGSNEGFSDSVKTNTALV
                     RKRLRTTDLKVEEIHFGARSDTVLALVYEKELIYPKFLEEVKQQIAGWEVDGVFDSGM
                     VEQLCEPQWKSPFPRFETTERPDRAAMEILDGRILLLCDNSPVGILFPASFDSFLKVS
                     EDRYHHFLIVSFERLLRYAAVFLALWISGGYLAVTGFHTQVLPTKLLLAFAEARKGVP
                     FPGILEILLMEFAFELIREAGVRMPGPLGGTIGIVGGLIIGDAAVSANLVSPMTVVVV
                     AVSALCSLAIPNEEFGAPFRLLKFGFILLGGWLGIFGMVLGTFLLAGHLAGLTSFGIP
                     YLMPFVGKGLAGYHAPKDSVWRPPMHQMRERPVFTKRDQRVRLRKNRKAAEPEEKQER
                     GE"
     gene            6518..8254
                     /locus_tag="PLN77_02935"
     CDS             6518..8254
                     /locus_tag="PLN77_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HRM43269.1"
                     /translation="MFSENDRISQRQMKRQIVLVLLCPAFWQLAAYGASDGLPGMAGI
                     FVGYGLLAVWCIYLVRLGGLYCRLESVMGKAGKLLLAVMYLCYLLFLGGTLLRRMAEL
                     AAAYLTAGVPQELCGLFLLAAAGFGVGSEVQKRGRLAEALYPWIVGGILILLLLTVPQ
                     MHVSSFEDAWKTEKSVEIVDNGILFGRNVWRTLENGTPIALLPFLLGHVQKEHRSVIL
                     PVTQSIWKTGLLVIAGSMALLGVFGRRGTAAAQDPLLLLMAGTSLPGGFLERFDILWM
                     AFLLMGLLFTLGSLLFYVSLIGEKTGLWKPQNLYVRIGTVLFIWLLSCAESGWSVRYG
                     YFPFFLAITVLLGFCQKHMKKLRLVCLALGSMIFLSSCGAVEPEKRLYPLAVAFDYVS
                     EEATAPENSETGEETGTAVSESGGEKETAGGKWRVWYAPANLAVVTGQGKDEQEEGTD
                     EDPASLFFEAETFEEIAQQYDSTEEYDLDTGHVQAIVFSDALLSDRGRMEELLKYLEQ
                     DRALGHSAYLFHAEKPEDIMEQNGKQVESVGEYLCGIYDNRVKRKEAVTLADLCYAWE
                     NERSIPWIPKAP"
     gene            8351..9097
                     /locus_tag="PLN77_02940"
     CDS             8351..9097
                     /locus_tag="PLN77_02940"
                     /inference="COORDINATES: protein motif:HMM:NF021093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HRM43270.1"
                     /translation="MNEKKVQSRKRNQNQTQKKSRDRQAQPRNTFGNQHRSQFQAAFQ
                     IFCRRWLPVCIAALILSGTANLLRESRLQQEVAAKIVRFHVRANSDCASDQQIKLQVR
                     DAVAEELQTILHGAETKAETEEILRENEPSIRAAALQTLRAGGSTDDITVTYGKASFE
                     EKETGSYILPAGTYDALQINIGRAKGHNWWCMLYPSICFSDALRPVNEDGESAEKVEK
                     SRIPLQNLLSDAAYREILKSDRISFRFFWR"
     gene            9295..10119
                     /gene="murI"
                     /locus_tag="PLN77_02945"
     CDS             9295..10119
                     /gene="murI"
                     /locus_tag="PLN77_02945"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRM43271.1"
                     /translation="MKADRQAPIGVFDSGVGGLTVVREIMRNLPDERIVYFGDTARVP
                     YGSKSKNTVIRYSRQIVHFLETQQVKAIVIACNTASALALDTIEKEIDLPIIGVVKPG
                     AQMAVETTQNKRVGVIATESTIQSGLYQQLITEADPAITVYGKPCPLFVPLVEEGWTK
                     DPITEEVARRYLAEILDKDIDTLIMGCTHYPLLRSLLRKVVGEKVTLVNPAYETSQAL
                     RRLLRELDLENPGNDELGRQKYQFFASDGVERFNAFAHSVLPEQISDTKQVQIENY"
     gene            10159..10611
                     /locus_tag="PLN77_02950"
     CDS             10159..10611
                     /locus_tag="PLN77_02950"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HRM43272.1"
                     /translation="MEKDVLISIRGLQYLMDEESGEQEPVEVMTTGSYYKKNGHHFLI
                     YDEATEGFDMMTHNMIKFTENALEVRKRGLIDVHMIFEEDKKNISFYHTPYGMINMGV
                     AATKVRMKETPDQILLKAEYALDINDSYVGDCVIDIEVRPREGGKLNL"
     gene            complement(10760..11215)
                     /locus_tag="PLN77_02955"
     CDS             complement(10760..11215)
                     /locus_tag="PLN77_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43273.1"
                     /translation="MKPWIIVLIVLAVIAVILGLLYYFGKKAQTKQAAQQEQMEAMKQ
                     TVSMLIIDKKKLRMKDSGLPQMVIDQTPKMMRRAKLPIVKAKVGPRIMTLICDAAIFD
                     QIPVKKEVKATVSGIYITGVRGIRGSLEQPAKKNGFWQKMLRKARGEEK"
     gene            11459..12694
                     /gene="tig"
                     /locus_tag="PLN77_02960"
     CDS             11459..12694
                     /gene="tig"
                     /locus_tag="PLN77_02960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRM43274.1"
                     /translation="MKRKAAVMLLLMTLGAASVSGCGKNNTTSSSSVSSASSTSASSA
                     SDASSAGSTSSDSTASEEYASPSDVDGIPVVDEEVDPAECVTLGDYKKLTLEKKVEQV
                     TDDDVQTAIMAETAVTMDDPDATVQKGDTADIAYVGKVDGKEFDGGSSDSYNLVIGSG
                     TFIDGFEDGVIGMKTGETKDLNLKFPDSYQSTDLAGKDVVFTVTVNAFTRPAENIDGW
                     ELDDAWVAKNTDYSTVEEYRAGKRAELEAANETTAENNLQDDAMKQVADGCTFTKIPK
                     SYVTLGEEDYDKRYTSYLQNYGISLDDYLEQYADRATYNQEKATYAGTMAKSALLLDA
                     VKEAEGWTTDDQDYQQILNDEAANANMSQEDFLKAANDYYGEDTVVRNIMMERMINMV
                     LDNATVNTVTVDADGNTVK"
     gene            complement(12933..14081)
                     /gene="fucO"
                     /locus_tag="PLN77_02965"
     CDS             complement(12933..14081)
                     /gene="fucO"
                     /locus_tag="PLN77_02965"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HRM43275.1"
                     /translation="MANRINLNATSYHGAGAIAEIANEAKAHGFKKAFVCSDPDLIKF
                     NVTSKVTDILTANGLDYELYSDIKPNPTIENVKHGVEAFKASGADYLIAIGGGSSMDT
                     SKAIGIIIANPEFEDVRSLEGTAPTKNPCVPIIAVPTTAGTAAEVTINYVITDVERKR
                     KFVCVDPHDMPIIAVVDPDMMSSMPKGLTASTGMDALTHAIEGYTTKAAWEMTDMFHL
                     KAIELISKSLRGAVANTKEGREGMALGQYIAGMGFSNVGLGIAHSMAHTLGAVYDTPH
                     GVACAMMLPIVMEYNQECTGEKYREIARAMGVAGVDEMSQEEYRKAAIDAVKKLSADV
                     GIPSVLEAVKEEDLQFLADSAHADACAPGNPKDASVEDLKDLFRKIMA"
     gene            complement(14305..14886)
                     /locus_tag="PLN77_02970"
     CDS             complement(14305..14886)
                     /locus_tag="PLN77_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HRM43276.1"
                     /translation="MIHSTVSAPKGEKTKYRLAESMKECMKTTPVEEITVRQICEICG
                     VTRQTFYRNFLDKYDLINWYFDKLLTKSFEHMGRGTTVFDSLEKKFTYIQKEKAFFAA
                     AFRYDKQNSLREHDFDLILAFYENLIHEKSGSPVSPEMHFLLEMYCQGSITMTVKWVL
                     TGMDLTPSEFAALLVRAMPEDLKKLFRNLQLLS"
     gene            complement(15022..16155)
                     /gene="codB"
                     /locus_tag="PLN77_02975"
                     /pseudo
     CDS             complement(15022..16155)
                     /gene="codB"
                     /locus_tag="PLN77_02975"
                     /inference="COORDINATES: protein motif:HMM:NF008241.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
BASE COUNT         4400 a         3710 c         4465 g         3651 t
ORIGIN      
        1 aaagtgtcct atactgaaaa gtagcaaaaa tacacaccac cctttcattt ggaggatttt
       61 tatgcagaag attacgtttc gaaaattagc tggagaaaaa tatatttacc cggaactgca
      121 gggacacttt atcgagtttc ttggctcctg tatttatgat ggaatctggg ttggagagga
      181 ttcggaaatt ccgaattatc atggaatccg aaaagacctt gtggatgcat ttcagaagct
      241 gcacccgccg gtgatccgct ggccgggcgg atgctttgcg gatgtgtatc actggcggaa
      301 tggtattggc ccgcgggagc agcggccggt tacatacaat gaaaacttcg gtactttcga
      361 gacggatccc aatcagttcg gtacacacga gatgatggaa ttttgcgaga tgatcggcgc
      421 gaagccatgg tttaatatca atatgatgac cggctctcca tccgagatgc gagagtggat
      481 ggagtactgt aaccgcaaag agccgacggc actttcgcag gaacgaaaaa caaacggcca
      541 tgaaaaaccg ttcgagattg aatactgggg tatcggaaat gaagtatggg acggcggtgg
      601 aaaaatgacg ccgcagatgt atgcgaacga ataccggaaa ttcagttcct cctgtccgtc
      661 gtttggaggc ggcgatcagg cattttccat gaaatgcatc gccagcggcc cggatggaaa
      721 taagccgaaa gaacgtgctg cgtggacaaa agatttcttt aaggaaatgg gaaaataccg
      781 gatgccgtct ctctatggct acgatctcca tttttacaac tggaatctga aacagcttca
      841 gacagagaaa aagtttgatg aaaaacagtg gtatgatgtg atcaatggat gcaaagagct
      901 ggaaaatgtc attcacgagc agcgccgcct gatagacgct ggtcttgagg cacttccgaa
      961 accggagggt ccgttccagg cggcaccgag aaaatgtgaa ctgattgtcg gtgagtgggg
     1021 caactggcac agcagtgcat ttaatgcgcg cccggcgctg tatcagcagt gtacgatgcg
     1081 cgatgccgtt acaactgcac tgacgctcga catcttccac cgcaataccg gggatgtgcg
     1141 catggcatgt gtggcacagt ctgtcaatgt cctgaattcc ctgttcctga cggatggtga
     1201 gcattgtatt ctgacaccaa actacgatgt gttcgatatg tatcaggtgc atcagggtgc
     1261 gtatacgctt ggctttgagg aaaagaacaa agatcctgag gtctgcatct ttgcttcgat
     1321 caaaaatgat gatatctacg tcaacctcgt aaatacaagc tacagcgaat caaagaaaat
     1381 cgaattgaaa tttaaacagt gtccggaatt tgttgaggcc aaaacactgt actccaaaga
     1441 tccgcagaac tacaatgatg cgaaacatcc gaaccgagtc cgctgcaaag aaggaaaagc
     1501 acctgcacgg gaaaaagact ccttccagat tgcactgccg gcggcttcgg tcagcgttta
     1561 tcatttcaga aaaacagaaa cagaaaagta atacatacaa aacgggccgc cccggggaaa
     1621 aggattgttc ccacagggcg gctcatttct attacaaaaa caattcaatc acaaacacca
     1681 cggcacagca tgccaccgaa acctgaaaca ggcttgcacc gaaccatgcg gctgcgatgc
     1741 cgacgacaag cgcgagtgcg ccggaaacag ggctctgtgt tgcatggaca atcgccggga
     1801 aggtcatgac tgacagcgtc acgtatggaa cgtagtacag aaacgactga ataaactgat
     1861 ttttaatcgg cttccggatc agcgtcagcg gcagaatccg gatggtgtag gaaaccaggg
     1921 ccatgacggc gatatagata taattattgt acatcagcat tttcctcctc tgtctttacc
     1981 gggaacagga tagctccggc gcaggaaatc agaacggtga gcagaatcgt tctggttccg
     2041 tcggagagag tggagatgcc cgggagatag ctgcagataa agcttaagaa aaagctgacg
     2101 gcaacaagac ccgctaccac acggctcttt cgcgccgggg gaatgatgat tgcaaggaac
     2161 atgccgtaca gcgccacgct taaggcactg acagcgcgca gcggcagcag atttccggca
     2221 ataataccaa gagccgtgcc gatcgaccac gcgggagcag cgacgagaat ggcaccgtat
     2281 gtataatacg gattcagcca gcccgggcgc gcgattgtga tgccaaacag ttcatccgtt
     2341 acatcgtaac caatgagaaa acggtggaaa aacggcgtgt ccggcgaaaa gcgctgggca
     2401 agcgcacagc tcatcagaag ataccgcgca ttggcgatga gcgtaatgat agcgacctgc
     2461 agatagcctg cgttggcggc aatcagtgta aaggcagcgt attctccggc ggaagcattg
     2521 ttgaaaagac tggcgagaaa gccctgaaaa ggactaagac ctgccttacg cgctgcaatg
     2581 ccgagcgaaa acgagaccgc aaaatagcca agcccgatcg gaatgccatc gcggattccg
     2641 ttgaccagaa cctggcggga aaatgaataa gatctttgtg aactcataaa aagctacttc
     2701 cttttccttc gcagaagcta gagcatgtct gaaaaatcat ttccgcaatc tgcaagcccc
     2761 actttatggt atattttgcc ctcattcggt tgccgtagcc cgctacgccg ccctcattcg
     2821 gacaaaatct cccacaaact gggactcgca gcttgcgaaa agcctttttc agacacgctc
     2881 tagagcgtgt ttgagaaaat tttccgtaat ctgagattcg cagcttacga aaaggttttt
     2941 tcagacatgc tctggcatct ggcaaaaatg ttggggtcaa gagatcaaaa atcctcttgc
     3001 aagcgagctt gcaccggctt tttgatcttt tgccctgata tctgatgaaa attatagcaa
     3061 gtttcgacag gttcgtaaac tcttattttg ttcttattcg tttcacgcag ccactgctta
     3121 cgcctgcgtt tctaagtctg ttgaactgaa ttgtataaaa ttatgtacaa aaacagaaga
     3181 taacgcttga aaatagaaga aaaatccatt aatatctatg tatacaaaaa acattaccga
     3241 aggtggtgct tacgatgaaa ctgcgtgcgc ttgcaaaaat aaatcttggt cttgatgtga
     3301 ccggaaaaag agaggatggt taccatgaag tccgcatggt aatgcagacg atccagatgt
     3361 acgatcagct ggaaataaag gagagcaaag aacccggaat aaggcttaca accaatctgc
     3421 cgttccttcc gtgtaatgac ggaaatctcg tatacaaagc ggcaaagatt ctgatggatg
     3481 aattcgatat ccgtcagggt gtcgatatga acctgacaaa atttatcccg gtagcggccg
     3541 gcatggcggg cggaagctcg gatgcggcag cagcactggt aggaattaac cgcatgtttc
     3601 agcttggtct gacaaaacgc cagctgatgg agcggggtgt gcagatcggt gcggatgttc
     3661 cgtactgtgt gatgcgcggt acagcgctgg cagaaggaat cggcgaaaag ctgacatccc
     3721 ttcccggcgt accgatgtgc tacgtgctga tcggaaaacc gggcatcaat gtttcgacga
     3781 aatttgtcta tggaaacctg catcttgatg aggtgacgga gcatccggat atcgacggca
     3841 tggtagaggc gatccggaat cacgatctgt acggcatcac ggaccgcatg ggaaatgtgc
     3901 tggaatccgt gacgtgtccg gcgtatccgg tgatcgatga gatcaaggca cagatgatga
     3961 aaaacggtgc ggtcaatgcg atgatgagcg gcagcggccc gaccgtattc ggcattttcg
     4021 acgatccgga caaggcagaa tttgcccgcg atcagttaaa aataagcgga ctgtcgaaac
     4081 agacattcct gactacagta tcaaaaccgg gaggtacaaa gtatggcaaa aaatgagtta
     4141 gacctgcaga tggaggatta tcagtacctt ccgctccgcg atgtcgtgtt ccgcacgctg
     4201 cgccaggcga ttctgcgcgg ggagttaaag ccgggtgaac ggctgatgga gatccggctg
     4261 gcaaatcagc tcggcgtttc gcgtaccccg atccgcgagg cgatccgtat gctggagctg
     4321 gacggccttg tgatcatggt gccgcggaag ggtgcgcagg tagcacagat cacggaaaaa
     4381 gacctgaacg atgtgctgga ggtgcggctt ggactggagg agctggcggt aaaactggcg
     4441 tgtcagcgga ttacagagag cgaactgcag aagctttatc aggcatcgcg cagctttgaa
     4501 caaatgctgg agacaacaga gacggatgat ctgcagaagc ttgcacaggc agatgtggca
     4561 ttccatgatg tgatttatca ggcgacgaac aacgagcgcc tgattcagct gctgaacaat
     4621 ctgcgggagc aaatgtaccg ttaccggatt gaatatttaa aggatgtaaa atcccgacgt
     4681 tccctggttg aggagcacga tgccctctat gaacgtatga aaaatcggga tcttgcaggc
     4741 gcacagaaga tgatccgcga gcatatcgag cgacagcagg agagtattat gcagaccgta
     4801 catcaccaga gcatgacggg tgtagaagaa aaataaaagc gtataaaatt tcgaaaaagg
     4861 aacattctgt tacagagtaa aaaaatcgtg acagaatgtt tttttatgga attttgcaga
     4921 gtatatttct ccagatggtt tttcggaagc tttcaaaagg aggagaagga gggacgcagg
     4981 catggaaaaa gagaagacgc agatttcact cacactggcg gaaaatgaaa gcgtgatccg
     5041 tacatggtgt gaaaattgcg atgatattca gatccgcccg atgaaactcg gcaaaacggg
     5101 cggaaccggt gcgctgctga tctacgtaga agtggcggtg agctgcgtta tgttgaagga
     5161 ttccgtgatc gggaagctgt tgaaccgtct gatggaggtt cccgaggcag atatcccggg
     5221 ggttctctcg gaaaatcagc tcggaatttc ggacacgctg gaatttgaca ccctcgagga
     5281 tgcctttgcc tcgatgctgg cgggaaacgc cgttctcttt gtggacggtt acgactgcgc
     5341 cgtcaaaatc ggttcgaagg gatatccaaa tatgggagtc cagaaagccg agtccgagaa
     5401 agtgctgcgc ggctcaaacg agggcttttc cgattccgtc aaaaccaaca cagcactcgt
     5461 ccgcaagcgt ctgcggacca ccgatctgaa agtggaggag atacattttg gtgcgcgttc
     5521 tgatacggtt ctggctctgg tgtatgagaa agagctgatt tatccgaaat ttctggaaga
     5581 ggtaaaacag cagattgccg gctgggaagt ggacggcgtt tttgacagcg gcatggtcga
     5641 gcagctctgc gaaccgcagt ggaaatcgcc gttcccgcgc ttcgagacga ccgaacggcc
     5701 ggaccgcgcg gcgatggaga ttctggacgg aagaatcctg cttttatgtg ataattcccc
     5761 ggtcgggatt ctcttcccgg catcatttga cagcttttta aaggtcagtg aggaccggta
     5821 tcatcatttt ctgatcgtct catttgagcg gctgctgcgg tatgcggcgg tctttctcgc
     5881 cctgtggatt tcgggcggat acctggctgt cacgggcttt cacacgcagg tgcttccgac
     5941 caaactcctg ctggcgttcg ccgaagcgcg gaaaggcgtt ccgttccccg gcattctgga
     6001 aattcttctg atggaatttg cttttgaact gatccgcgag gccggtgtgc gcatgccggg
     6061 gccgcttggc gggacgatcg gaattgtggg cggactgatc atcggagatg cggcggtttc
     6121 cgccaatctg gtgagcccga tgaccgtcgt tgtggttgcg gtttcggcgc tctgttcgct
     6181 ggcgattccg aacgaagagt tcggcgcacc gttccggctg ctgaaatttg gctttatttt
     6241 gctcggcgga tggctcggca ttttcggcat ggtgctcggc acatttcttc tcgcgggtca
     6301 tctcgccggg cttacgagtt ttggcattcc ctatctgatg ccgttcgtcg ggaaaggact
     6361 tgcgggctac catgcgccga aggattccgt atggcggccg ccgatgcatc agatgcggga
     6421 gcggccggtc tttacaaagc gcgatcagag agtgcggctg cggaaaaaca gaaaagctgc
     6481 ggagccggaa gaaaaacagg aaaggggaga gtgacgcatg ttttcggaaa atgacagaat
     6541 ttcacagcgc cagatgaaac gccagatcgt gctcgttctg ctctgcccgg cattctggca
     6601 gctcgcagcg tacggcgcat cggacggcct gcccggtatg gcagggattt tcgttggata
     6661 tgggctgctg gcggtgtggt gcatttatct cgtgcgcctt ggcgggcttt actgccgcct
     6721 ggaaagcgtg atgggaaagg ctgggaaact ccttctggca gtgatgtatc tgtgttatct
     6781 gcttttcctc ggcggaacac tgcttcgcag aatggcggag cttgccgccg cgtatctgac
     6841 agcgggtgta ccgcaggagc tctgcggact gtttctgctc gcggcggccg ggtttggtgt
     6901 cggcagtgaa gtacagaaaa gaggacggct ggcggaagca ctctatccgt ggattgtggg
     6961 cgggattttg attctgcttc tgctgacggt tccgcagatg catgtttcct cgtttgagga
     7021 tgcctggaag acagaaaagt ctgttgaaat tgtggataac ggaatacttt ttgggcggaa
     7081 tgtgtggaga acactggaaa atggaacacc gattgcgctg ctgccgtttt tactggggca
     7141 tgtgcagaaa gagcacagga gtgtcattct tccggtgacg cagagtatct ggaaaacggg
     7201 gcttcttgta attgcaggaa gcatggcgct gctcggcgtt ttcggccggc gcggcacggc
     7261 tgcggcgcag gacccgctct tactgttaat ggcaggaacg agcctgccgg gtggatttct
     7321 ggagcggttt gatattctgt ggatggcatt tcttttgatg ggactgctgt tcacactggg
     7381 aagtctgctg ttttacgtat cgctgatcgg agagaagacg ggactgtgga aaccgcagaa
     7441 tctctatgta cggatcggta cagtgctttt tatatggctg ctgtcatgcg cggaaagcgg
     7501 ctggtcggtc cgatacggtt actttccgtt tttccttgcc atcacggtgc tgctcggctt
     7561 ctgccagaag catatgaaaa aactgcggct tgtgtgtctg gcgctgggca gtatgatttt
     7621 cctgtcatcc tgcggggcag tagagccgga aaagcggctg tatccactgg cggttgcctt
     7681 tgattatgtg tcggaagagg cgactgcgcc ggaaaacagt gaaactgggg aagaaaccgg
     7741 gacggcggtc agtgagagcg gcggagaaaa ggagacggct ggcggtaaat ggcgcgtctg
     7801 gtatgcgcct gcgaatcttg cggtggtgac cggccaggga aaggatgagc aggaggaggg
     7861 aaccgacgag gaccccgcat cgctgttctt tgaagcggag acgtttgagg agattgcgca
     7921 gcaatatgac agcacagagg aatatgatct cgacaccgga cacgtccagg cgattgtatt
     7981 ttccgatgca ctgctcagtg accggggacg gatggaagaa ctgttgaagt acctggaaca
     8041 ggaccgtgcg cttgggcaca gtgcctatct gtttcacgca gaaaaaccag aagatatcat
     8101 ggagcagaac ggaaaacagg tggagtccgt cggtgagtac ctgtgcggaa tttatgacaa
     8161 ccgggtgaaa agaaaagagg cggtgacact ggcggacttg tgctatgcgt gggaaaacga
     8221 aaggagcatt ccgtggattc cgaaagcgcc gtaaaaggtt taaagtgtat ggaatatcgg
     8281 agcgggcagt gagtgtctgt gtataaaagt tttgcaatcc ggcaaaactg gaaaaaacag
     8341 gaggcagcat atgaatgaga aaaaagtaca gagccggaaa cggaatcaaa atcagacaca
     8401 gaagaaatcc agggacaggc aggcgcagcc ccgaaataca tttgggaacc agcatagaag
     8461 tcaattccag gcggcatttc aaattttctg ccgccgctgg ctccccgtct gcattgcagc
     8521 gctgatcctt tccggaaccg ccaatcttct ccgcgagtcc cgtctgcagc aggaagtcgc
     8581 tgcaaagatc gtgcgctttc acgtaagagc caacagcgac tgcgcctccg accagcagat
     8641 caagcttcag gtacgcgatg ccgttgccga agaactgcag acgatcctgc acggagccga
     8701 aacaaaagca gaaaccgaag aaatcctgcg tgaaaacgag ccctccatcc gtgccgccgc
     8761 ccttcagacg ctgcgcgcag gaggcagtac tgacgatatc accgtaacgt acggaaaagc
     8821 cagttttgag gaaaaagaaa caggcagtta tattctcccc gccggaacgt acgacgctct
     8881 tcagatcaac atcggcagag ccaaagggca caactggtgg tgcatgctgt acccgtccat
     8941 ctgcttttcg gatgccctgc gccccgtaaa cgaggacggc gaaagtgcag aaaaagtcga
     9001 aaaaagccgg attccgctgc agaatctgtt gagtgacgcc gcctaccgcg aaatcctgaa
     9061 aagtgaccgg atttcgttcc ggtttttctg gcgttaagcg gatttttcga aagactttca
     9121 agagaaatga ttgcaatatt cctccattcg ggttaaaata atgtatcagc gacctatttt
     9181 aaccagggtc gtgaaatgaa aaaagaaatg taaaaattca gatatattaa atatagaaat
     9241 gaaccccatt cggcatgaca aaaaagtaat gccggatgaa aaatggagga aagtatgaaa
     9301 gcagacagac aggcgccgat cggcgtcttt gattccggtg tcggcggact gaccgtagtg
     9361 cgtgagatta tgcgaaacct gcccgacgaa cgaatcgtat attttggaga taccgcgcgt
     9421 gtgccgtacg gttccaaatc gaaaaatacc gtcatccgtt attcccgcca gatcgtacac
     9481 tttctggaaa cccagcaggt caaggcaatc gtcatcgcct gcaacaccgc gagcgcgctg
     9541 gcactcgata cgatcgaaaa agagatcgat ctcccgatca tcggtgtggt aaaaccgggt
     9601 gcacagatgg cagtcgagac gacgcagaac aagcgcgtcg gcgtcatcgc aaccgagagc
     9661 actattcaga gcggcctcta ccagcagctc attaccgagg cagatccggc gatcaccgtt
     9721 tacggaaaac cgtgtccgct gttcgtgccg ctcgtggaag agggctggac aaaagatccg
     9781 atcaccgagg aagtggcgcg ccgctatctg gcagagattc tggataaaga catcgacacg
     9841 ctgattatgg gatgcaccca ctatccgctg ctgcgttctc tgctgcggaa agtagtcgga
     9901 gaaaaagtaa cactcgtcaa cccggcatac gaaacctcac aggcactgcg cagactgctg
     9961 cgggagctgg atctggagaa tccgggaaac gatgagctgg gccgtcagaa ataccagttc
    10021 ttcgcaagcg acggcgtgga gcggttcaat gccttcgcac attccgttct gccggagcag
    10081 atcagcgata cgaaacaggt acagattgaa aactattaaa aacgacatac agaaaagaga
    10141 gaggaacacc agaaaaggat ggaaaaagac gtactcatca gcatccgcgg actgcagtat
    10201 ctgatggatg aggaatccgg cgaacaagaa ccggtggagg ttatgacgac gggttcgtac
    10261 tacaagaaaa acggccatca ctttctgatc tacgacgagg caaccgaagg ctttgacatg
    10321 atgacgcaca acatgatcaa atttacggaa aacgccctcg aggtgcgcaa gcgcggcctc
    10381 attgatgtcc atatgatttt tgaggaggat aaaaagaaca tttcgtttta tcacacgcca
    10441 tatggcatga tcaacatggg agttgcggcg acgaaggtca gaatgaaaga gacgccggat
    10501 cagattttac tgaaggcaga atacgcgctc gacatcaacg acagctacgt gggggactgc
    10561 gtaatcgata tcgaggtaag accgcgggaa ggcggaaaac tgaatttata aaaaaatacc
    10621 cgggaagaat gaaaatcatt caactcgggt attttcgcga gcaacgagcc aaagtccggg
    10681 cttgcacgag accttggcga ctgccgcgaa agcaaaaaga aacacgcaaa gcgtgtttct
    10741 ttttgtttca gatgaaattt tatttctcct cgccgcgtgc cttgcggagc attttctgcc
    10801 agaagccgtt tttctttgcc ggctgctcta aagaaccgcg gatgccgcga acgccggtga
    10861 tgtagatacc gcttacggta gccttgactt cttttttcac cgggatctga tcgaagatag
    10921 cagcgtcgca gatcagggtc atgattctcg gaccgacttt tgctttgacg atcggcagtt
    10981 ttgcgcgacg catcattttc ggtgtctggt caatgaccat ctgtgggagt ccggaatctt
    11041 tcatgcgaag ctttttctta tctatgatca acatggagac agtctgtttc atggcctcca
    11101 tttgttcctg ctgtgcggcc tgttttgtct gtgctttttt accgaagtaa tacagcagac
    11161 caaggatgac agcgatgaca gctaatacga ttaatacgat aatccagggt ttcatccctc
    11221 tattcctcct ctgcaaaaaa taatgtgggc atcggaacat acaatgccag tctatgaaca
    11281 ttctaacatt gatttcggca aaacgcaaac acattttagc gcaatccagg cgattttacg
    11341 gtcgaaaatc ataagaattt ctaaaatcgg agccccagcc ttttttttac cagacaagta
    11401 tggtaaaatg agatttacaa cgcgccggga acggcgtagt atggaggaaa attcaagaat
    11461 gaaaagaaaa gcagcagtca tgcttctgct catgacactg ggagcagcca gtgtgagcgg
    11521 atgtggaaaa aacaatacaa caagcagcag ttccgtatca agcgcaagca gcacaagcgc
    11581 ttcctcagca tccgatgcgt cgagcgcagg cagcacatcg tccgacagca cagcttctga
    11641 ggaatacgct tccccgagtg atgtagacgg gattccggtt gtcgatgagg aagttgatcc
    11701 ggcagagtgt gtaacgcttg gagattacaa aaagctcaca ctggaaaaga aagtagagca
    11761 ggtaacggat gatgacgtac agacggccat tatggcagag acagctgtca ccatggacga
    11821 tccggatgcc accgtacaaa aaggcgacac cgccgatatt gcctatgttg gcaaagttga
    11881 tggcaaggag tttgacggcg gaagcagcga ttcctacaat ctggtgatcg gatccggtac
    11941 cttcatcgat ggatttgagg atggtgtcat tggaatgaaa acaggggaga caaaggatct
    12001 gaacctgaaa ttccctgaca gctatcagag taccgatctg gcaggaaaag atgttgtgtt
    12061 taccgttacg gtaaatgcgt ttacccgccc ggcagaaaac attgacggat gggagctcga
    12121 tgatgcatgg gtagcaaaaa atacagatta cagtactgtg gaggaatacc gtgccggaaa
    12181 acgcgcagag ctggaggctg caaatgagac gacagcggaa aataatttgc aggacgatgc
    12241 gatgaagcag gtagcagacg gctgtacctt cacaaaaatc ccgaaatcct acgtcactct
    12301 cggcgaggag gattacgata aacggtatac cagctatctg cagaattacg gcatttccct
    12361 ggacgactat ctggagcagt atgcggacag agcgacctac aatcaggaga aagcaacgta
    12421 tgcaggaacg atggcaaaaa gtgcccttct tctggatgcc gtaaaagagg cagaaggctg
    12481 gacaacagat gaccaggatt accagcagat cttaaacgat gaggcagcca atgccaatat
    12541 gtcgcaggaa gatttcctga aagcagccaa tgactattac ggggaagata cggtagtgcg
    12601 caacatcatg atggagcgca tgatcaatat ggttctcgac aatgcaacag tcaataccgt
    12661 gactgtggat gcagacggaa atacagtaaa ataaaaagaa aaatcacccc ggaaaacagg
    12721 atgaaaactt ctgttttccg gggtgattta accgaatcaa gtaagtcccc tgcttccatt
    12781 gcgaaaagca ataagcagtg ggtggggact tgcttgtatc aggaggagtg caagctcctt
    12841 ctgataaact gcggagcagt tattcggtta tgagcaagta gcgaagcgga ttgcgaatat
    12901 ccgcttcgca tgtgggaaac gaattatgtt cgttacgcca tgattttgcg gaacagatcc
    12961 ttcagatctt ctacgcttgc atccttcggg ttgcccggtg cgcaggcatc tgcgtgtgcg
    13021 gaatctgcaa ggaactgcag atcttcttct ttgactgcct cgagaacaga tgggattcca
    13081 acgtctgcgg acagcttctt aacagcatca atcgctgctt ttctgtactc ttcctgagac
    13141 atttcgtcta cacctgcaac gcccatggca cgtgcgattt cgcggtattt ctcaccggtg
    13201 cactcctggt tgtattccat aacgatcgga agcatcatgg cacatgcaac accgtgtggg
    13261 gtatcataaa cagcaccaag ggtgtgtgcc atggagtgag cgattccaag acctacgttg
    13321 gagaagccca tacctgcgat gtactggcca agagccattc cttcgcgtcc ctctttggtg
    13381 tttgcgacag caccgcgcag agatttggag atgagttcga tggctttcag atggaacatg
    13441 tcagtcattt cccatgcagc ttttgtggtg tagccttcga ttgcgtgagt cagagcgtcc
    13501 ataccggtgg aagcggtcag gcctttcggc atggaggaca tcatatccgg gtcaacaact
    13561 gcgatgatcg gcatatcatg cgggtcaacg catacgaatt tacgttttct ttctacgtcg
    13621 gtgataacgt agttgatggt aacctctgca gcagtaccgg ctgtagtcgg aacggcgatg
    13681 atcggtacac acggattttt ggtcggtgcg gtgccctcaa ggcttctgac atcctcaaac
    13741 tccgggtttg cgatgatgat accgatggct ttggaggtat ccatagaaga gccgccgccg
    13801 atggcgatca gatagtcagc gccggatgcc ttgaaagctt ctacgccgtg ttttacgttt
    13861 tcgatggtcg gatttggctt gatatcgctg taaagctcat agtcgagtcc gtttgcggtc
    13921 agaatatcgg ttactttgga agtaacgttg aatttaatca gatccgggtc agagcatacg
    13981 aaagctttct tgaagccgtg tgcctttgct tcatttgcga tctcggcaat agcgcctgct
    14041 ccgtgataag aggttgcatt taaattaata cgatttgcca tgattgaaaa actcccttct
    14101 aaaatgaata aatggtttta ctgaacttgt tattatatta acaaatccgg aagggaaaaa
    14161 gaaagtgaca gaagaagcga agtgtaacac tttaaaaaaa atcgccattg tgacaaaaac
    14221 aacagaacaa aatttgtgaa aaataaccag agcgtgtctg aaaaaccatt tccgcaatct
    14281 gcaagcccca ctttgacacg cgctctaaga gagcagctgc agattccgga acagtttttt
    14341 taagtcttcc ggcattgccc ggacgagaag agcggcaaac tcggaaggtg tcagatccat
    14401 gccggtaagc acccatttta cggtcatggt gatggaaccc tgacagtaca tttcgagaag
    14461 aaaatgcatt tccggtgaaa ccggactgcc gcttttttcg tgaatcagat tttcgtagaa
    14521 cgcgagaatc agatcgaaat cgtgctcacg cagagaattc tgcttgtcgt agcggaaggc
    14581 agcggcgaaa aaagcttttt ctttctgaat ataggtaaat ttcttttcca gactgtcaaa
    14641 aacggtcgtt ccgcgaccca tatgttcaaa ggattttgtc agtaatttat caaaatacca
    14701 gttgatcaga tcgtatttgt cgagaaaatt gcggtagaag gtctggcggg taacaccaca
    14761 gatttcacag atctggcgca cggtgatctc ttcaacgggt gttgttttca tgcactcctt
    14821 catagattcg gcaagacggt attttgtttt ttctcctttg ggagcagata cggtggaatg
    14881 gatcatccgg gcagtttcct ttcgtttcga aaattataac tatgcagagt tatgcataca
    14941 ttataacgcg aatcagaaga aataaaaagc cctgaacatg tttccaggat gaaagcgaga
    15001 aacatgccaa gggcgttatt gttattttct tttgtttacg gcctgtccga cacagtagca
    15061 tacagcggca acaaccatgc cgttgatgga agcgatgccc cagtgtaaga ggttcgcaac
    15121 aatggcaccg agaatgacac cggcgaccgc aaaccagtct atggttttaa gcttcggctg
    15181 atcggtttca aagtcctctt tgttcatgaa gtaggcgagc accagaatca tgccgaccgg
    15241 cggcagggta caattcagaa tgttgagcca gccgcagaag ttgtagtaca gccatacgga
    15301 ggcaaccgtt ccgatgatac cggaaaggag aaccatcggt tttttcctct gatggaagat
    15361 gttggcaaga ccaagacctg cggtatacag agcgttgtcg tttgtagtcc agatattaag
    15421 accgagaacg agaacagcca ggtagaacag gttcaggcgg accataacct cgaagatatc
    15481 gtttccgccg acaaagatcg aggaaacggc accgaagaag aacatcaggg agtttccgat
    15541 gaagaaggcc acaaccgtgg tgatggcacc cgcttttccg tcctttgcaa aacgtgcgaa
    15601 gtttggagtt gcggttccgc cggaaacgaa ggagccgatg acaagaccgg caccgccgat
    15661 gatagtcagc gatccgctgg attttgcaaa ctgatcgatg atcgttccgt cgcctctcat
    15721 tacggccatg accatggcga tcgttccgag aatggcaaca cttggtacag cgatatagct
    15781 gacgatggtc agagattcaa taccaaaata ggcagaggcg gtcatgcaga cgccggcaag
    15841 aataacaagt ccccactgtg ccgcagtgct tccgccgagc agctccttgg aaaccggaat
    15901 tgcaaacatg gcaagaccga caccaaacca tccgatctgc gtaaagctgg tcatggctga
    15961 cggcagatag gaaccttttt cgccaaaagc tctctgggac aaaagatcaa gtgacagacc
    16021 ggtttttgca ccgacgtagc cgagcagacc ggtataaata ccaagaatga ttccaccaag
    16081 gatcagtgat cccaaaaagc cccagaaatc gaggccgtca gcgagctgct ggcctaccca
    16141 catgcttgcc gagaagaagg tgaagccaag ctataaactt ttaacagtgt aaggggtagt
    16201 aacctgattg tcaagggaaa aggcga
//
LOCUS       DAOMUF010000026        16168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_726_length_16168_cov_4.069075, whole genome shotgun sequence.
ACCESSION   DAOMUF010000026 DAOMUF010000000
VERSION     DAOMUF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 16168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16168
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_726_length_16168_cov_4.069075"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1418
                     /locus_tag="PLN77_02980"
     CDS             114..1418
                     /locus_tag="PLN77_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HRM43277.1"
                     /translation="MKKKLVVALISAAMVTSMLGACGSSNASSSSASSTATSSVSASS
                     ASEEDTTAASSSVSASSASEEVSADADQEAADKVAALIDAIYVQERNDNTDEQCKEAK
                     EAWDALTDAQKELVEGENADPDYFGRDTGDASKDDPLNGDEIGENELLVVSFGTSFND
                     SRAEDIGGVEKALQAAYPDWSVRRAFTAQIIINHVQARDDEKIDNVDQALERAVSNGV
                     KNLIIQPTHLMHGAEYDELKEAVDSYKDKFESVTIAEPLLGEVGSDATVINEDKQAVA
                     EAITAQAVKSANYDSLDAAAEDGTAFVFMGHGTSHTAKVTYSQMQTQMDTLGYKNVFI
                     GTVEGEPEETACENVIEAVKAAGYKKVILRPLMVVAGDHANNDMAGEDEDSWKSQFEA
                     SGAFDEIDTQIEGLGRIDAVEQLYVQHTKAAMDGNGVQDDAE"
     gene            1558..2169
                     /locus_tag="PLN77_02985"
     CDS             1558..2169
                     /locus_tag="PLN77_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43278.1"
                     /translation="MRKKLAVLFLGAALTVSMLGACGGKDAASSSASAVSASSAADAS
                     SASVQDASKAESSTSTSDVSAESASAAETAGESSASELEDGTYTADFNTDSNMFHVNE
                     AKEGKGVLTVKDGKMTIHVSLVSKNIVNVFVGTKEEAQADGAELIEPTTDEVTYSDGT
                     TDEVYGFDIPVEKLDEDFNVAIIGKKGTWYDHVVSVSNPVPQE"
     gene            complement(2281..2739)
                     /locus_tag="PLN77_02990"
     CDS             complement(2281..2739)
                     /locus_tag="PLN77_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HRM43279.1"
                     /translation="MPKTKFQNVVFTLMMSFLMVYAMICYNISMNIGGMTNQVFLMAF
                     HEMIIMWPAAFILEFFLVDHLAHKLAFCMVTPQDRPIVITLAISIMIIAIMCPIMSFI
                     ATLLFKNAGKEFVAVWLQTTFLNFPVAFFWQLIYCGPFIRFLFRKLFPEK"
     gene            3099..3923
                     /locus_tag="PLN77_02995"
     CDS             3099..3923
                     /locus_tag="PLN77_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRM43280.1"
                     /translation="MSEIRKIENFAAPELDVYARLSEPQLLHYYEPQPGLFLAESPRV
                     IERALDAGYEPVSFLAGSAELAANEALFAHCPDAPVYTAETKVLEQLTGFALTRGMLC
                     AMHRRTLPAMEEICRNARRVAILENVVNPTNVGAIFRSAAALGIDAVLLTSGCSNPLY
                     RRAIRVSMGNVFQVPWTVLPGGSYWPEQGIAALQELGFYTVAMALKEDSVPMDDPSLK
                     THEKLAILLGTEGDGLASDTIAETDATVLIPMTHGVDSLNVAAASAVAFWELAGRR"
     gene            complement(4015..4890)
                     /locus_tag="PLN77_03000"
     CDS             complement(4015..4890)
                     /locus_tag="PLN77_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM43281.1"
                     /translation="MMLSAAKEKRQHGSMQVPYSYYDCRMPDYFPGVPLHWHGEMELN
                     YIKRGTGYFQYEDRLLTAHAGDIFLIQPNLVHAILPAEQESMFYDTIVFQQNMLIGGY
                     DDRSYTEILQPFFSARRRIQAPISPEHSGYTALSASVHQIFRCIRENSAVSDLLLKSE
                     LLRFFYLLAASPELCTDRPAMPAAAENLRPVLAYIQEHFADPITIEELARLSHLSKSY
                     FMSCFKQTFGLGAVEYLTQLRIKAACEALRSTTRSVSEIAYDNGFRNLSNFNRQFRNK
                     VGCTPQMYRKEFQNS"
     gene            5241..6785
                     /locus_tag="PLN77_03005"
     CDS             5241..6785
                     /locus_tag="PLN77_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM43282.1"
                     /translation="MSKWLDNAVFYEIYPQSFNDTNADGIGDFQGIIEKLDYIKELGC
                     NAIWINPCFLSPFGDAGYDVADYCRVAPRYGTNEDLKRVFEEAHKRDMHVLLDLVPGH
                     TSVEHPWFKESMKADRNEFTDRYVWTNNVWEAPEGMGSLRGISERDGACAINFFSNQP
                     ALNYGFYQPDPEKPWQQSFDDEGPQATLAAMEDVMRFWLNMGCDGFRVDMAGSLVKND
                     QEGKGTIRLWQQVRKFLDAEFPDAAMVSEWGEPDKSLQGGFHMDFLLHFGPSHYNDLF
                     RCDEPFFSKRGKGDISEFVETYKTNYNKTAQKGLICIPSGNHDMDRLARRLQGDELKI
                     AFAFLLSMPGAPFIYYGDEIGMRYVEGLKSVEGGYNRTGSRSPMQWDDSTNAGFSSAP
                     ASDLYITMDPGKDRPTAKAQMADPDSLYHEVKKLIQIRQSSKALLSRGTIEFVYAEKN
                     QYPLAYVREAEGEKVLVIINPSDKEQSFPYSAELKDALYTIGGKAESKDGTITIPAQS
                     AGFYHI"
     gene            complement(6844..8895)
                     /locus_tag="PLN77_03010"
     CDS             complement(6844..8895)
                     /locus_tag="PLN77_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM43283.1"
                     /translation="MTDQKNGQEYLSFVLEKLQARLAEISQSLLDGQKEIENMHDYYW
                     QNYTEMDQYGYEDYDNQQALLHQVNANQEQLLLRSRFRKMLDSPFFGRVDFRYDGDDE
                     PEIFYIGIGNFAERPGELPLIYDWRSPVSGLFYDFDRGPASYLAPGGEMTGEICSKWQ
                     YKIRDGKMIYGFESDVKIDDDILKAELGSNGEVQLKNIIRTIQKEQNAIIRNTKDRIL
                     VIQGAAGSGKTSVALHRIAYLLYHDRQNLKSSNILILSPNGVFSDYISHILPELGEEN
                     IQEMSFDLFAYRKLQDTAADCEDRCDQIEREMRDPKAAERFALKQSQAFVDQMEGFAL
                     ELEDELMNFSDVSYKSFVKSESEIITLFYDKFADIPLLSRMDAVAETFIDEIETLLNR
                     DLPEEERIPLIEKFQKMYETMDFYVLYNRFLKKEGYQTLPRRPLEKRKLRYEDVYPVL
                     YLKYRLSRQAERSNIKHLVIDEMQDYSRLQYLIIRRMFSCKMTILGDRAQTMADQQQD
                     VLQFLPGIFGKDLRRIEMRKSYRNTVEIASYAANLIGVTDLELFERHGMPVLERDVTD
                     LEAALREAVDTLFPEEKTYETAAVIVPDEKTAERAYLILREILAEKDFDCEKRLSWLN
                     RDSSSFKKGLTVTTFYLAKGLEFDQVFSIFPKDEKREMMMQAQYIAATRALHELRVYH
                     I"
     gene            9032..9658
                     /gene="sigK"
                     /locus_tag="PLN77_03015"
     CDS             9032..9658
                     /gene="sigK"
                     /locus_tag="PLN77_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HRM43284.1"
                     /translation="MEPFLKPLSAQEEKSCLDAYQRGDTKARDILVERNLRLVAHVVK
                     KYQGLPDDLDDLISIGTIGLIKAVMTYDSKKGNRLAAYAARCIDNELLMYLRSKKKTA
                     REISLYEPIGTDKEGNEIHLLDIVEGGGEDIAEQYNRKEDAVRLYCYLDSELKEQEKQ
                     VLLLRYGLGGKAEYTQKQVAARLGISRSYVSRIEKNAIEKLRLRFAAS"
     gene            9854..11377
                     /locus_tag="PLN77_03020"
     CDS             9854..11377
                     /locus_tag="PLN77_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRM43285.1"
                     /translation="MYCSVLSATVSGMEMIPVQVEADVSDGMPQFTMVGYVSAQVKEA
                     QDRVRTALKNMGISLPPKRITINLSPADVRKEGSRFDLPIAAGVLMTLGRIPPGSLRK
                     TMVLGELGLDGHVQEISGVFPAAAKARELGCTTLLVPAGNAAEGGLVGGLHVVGIQNL
                     QELLNYCCEGKMPSLPSIENQKKEDGKEPDFSEVQGQTAARRAALIAAAGFHNLLMLG
                     PPGSGKSMIARRIPTIMPPMSEEEQMEVTRIYSIAGMLAKGEGVRRERPFRAPHHTMT
                     PQALAGGGKQPSPGEITLAHRGVLFLDELPEMTRTTIEILRQPLEEHRIVISRVSGNY
                     VFPASFLMVAAMNPCPCGFYPDLSRCTCAPGDISRYLSRVSQPFLDRMDLCVQVEALS
                     YEDLHGEERCESSAKMREKATAAAEIQAARYRQESIHFNSELKAGQIEKYCILEKAAE
                     ELLEDAFRRLRLSARSYHGIIRTARTIADLDGAVKIGVPHISEAICFRSADKSIWRI"
     gene            11381..12250
                     /gene="dprA"
                     /locus_tag="PLN77_03025"
     CDS             11381..12250
                     /gene="dprA"
                     /locus_tag="PLN77_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRM43286.1"
                     /translation="MAMEIFTVGRGQEGYPERLMPFADMPSRLFVRGALPADEQKTAA
                     IVGARICTAYGKSQAAFFAQVLAANGVAVISGLACGIDAAAHEGALRGKGKTFAVLGC
                     GVDICYPKQNYPLMRRMLENGGGVLSEFPPGAEPLPWHFPIRNRVISALADVVLVIEA
                     KEKSGSLITADYALEQGKTVFALPGRTTDATSRGCNRLIAQGAEIAISPETILEELGV
                     KKAGTKRAAPRPRYGKEAEMVLKTLAAEPQNLAQLAGSTGIPLQELTGILLALEIDGV
                     IAENPPGVYMALS"
     gene            12618..14699
                     /gene="topA"
                     /locus_tag="PLN77_03030"
     CDS             12618..14699
                     /gene="topA"
                     /locus_tag="PLN77_03030"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRM43287.1"
                     /translation="MAKYLVIVESPAKVKTVKKYLGANYEVAASMGHVRDLPKSRLGI
                     DVEHDFEPKYITIRGKGELLASLRKAAKKADKVYLATDPDREGEAISWHLSIALNLDE
                     NKMRRITFNEITKSAVKASIKQARDIDMNLVDEQQARRCLDRMVGYEISPLLWKKIKR
                     GLSAGRVQSVALRIIGDREDEINAFIPKEYWTLEADLKIPGEKKPLVAKFHGKGSEKM
                     AVETKEQLDEILKGLENETFTVSEVKKGERSKKAPLPFTTSTLQQEASKVLNFSTQKT
                     MRLAQQLYEGVEVKGKGTIALISYLRTDSTRISEEADAAARSFIQEQYGTKYTASGDT
                     TENKNSKAQDAHEAIRPTDVTNTPVMVKESLPRDLFRLYQLIWKRFVASRMQPAVYET
                     ISARINAGEYLFTAASSRLAFDGFMSVYTDPDEEKKEKNAAVRSLEAGTELALEKMDP
                     QQHFTQPPAHYTEASLVKTLEELGIGRPSTYAPTITTIINRRYVAKENKNLYMTELGE
                     VVNNMMKEAFPSIVDVNFTATMEALLDRVGDGSVPWKTVVENFWPDLYEAVTEAEKNL
                     EEVKVEDEVTDVICENCGRNMVVKYGPHGKFLACPGFPDCKNTKPYLEKIGVPCPKCG
                     KEVILRKTKKGRKYYGCENNPECDFMSWQKPSAKKCPQCGGYMLEKGNKLVCADEQCG
                     YVEAREKKDED"
     gene            14959..15780
                     /gene="codY"
                     /locus_tag="PLN77_03035"
     CDS             14959..15780
                     /gene="codY"
                     /locus_tag="PLN77_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HRM43288.1"
                     /translation="MSVQLLDKTRKINKLLHNNHSTKVVFNDICEVLMETLSSNILVI
                     SKKGKVLGISLCPGVEEITELIEDKVGGYVDAALNERLLGVLSTKENVNLQTLGFERP
                     GVERYTAIICPIEIAGERLGTVFMYRCTPQYDIDDIIVSEYGTTVVGLEMMRSVNEEN
                     VEENRKVQVVKSAFNTLSFSELEAIIHIFDELNGDEGILVASKIADRVGITRSVIVNA
                     LRKFESAGVIESRSSGMKGTYIKVLNDYIFDELDEIKARKNLKGKGDAHPDEAKK"
BASE COUNT         4559 a         3892 c         4269 g         3448 t
ORIGIN      
        1 aactgccgcg ataacttgag aaactcgcgg agcgtgtttc tcatagtttg aaatgtaaaa
       61 cgtccccgat attggttgtt gcaaagatac cagcaaatgg gaggaaaaca aaaatgaaaa
      121 agaaacttgt agtcgcttta atttcagcag ctatggtaac atctatgctc ggcgcatgcg
      181 gaagcagcaa cgcatccagc agcagcgcaa gcagcacagc aacaagcagc gtaagtgcat
      241 cttccgcatc tgaggaagac accacagcag caagcagcag cgtaagcgca tcttctgcat
      301 ctgaagaggt atccgcagat gcagaccagg aggcagcaga taaagtggca gctctgattg
      361 acgcaattta tgtacaggaa agaaatgata acaccgacga gcagtgcaaa gaagcaaaag
      421 aggcatggga tgccctgaca gacgcgcaga aggaacttgt agaaggcgag aacgcagatc
      481 cggattactt cggaagagac acaggtgatg catccaagga tgatccgtta aacggagacg
      541 agatcggcga gaatgagctt ctggttgtaa gctttggtac ttccttcaac gacagccgtg
      601 ctgaggatat cggaggcgtt gagaaagctc tgcaggctgc atatccggac tggtccgtaa
      661 gacgtgcctt caccgcacag atcatcatca accatgtaca ggcccgcgat gacgagaaga
      721 tcgacaacgt ggatcaggca ctggaaagag ccgtaagcaa tggcgtgaag aacctgatta
      781 ttcagccgac tcatctgatg cacggcgcag agtacgacga gctgaaagaa gcagtagata
      841 gctataaaga taaatttgag tccgtaacca tcgcagagcc gctgcttggc gaggttggaa
      901 gtgacgctac cgtaatcaac gaggacaaac aggccgttgc agaagcaatc acggcacagg
      961 cagtaaaatc tgcaaattat gacagtctgg atgcagcagc agaggacggc actgctttcg
     1021 tatttatggg acatggtact tcccacactg caaaagtaac ctacagccag atgcagacac
     1081 agatggatac tcttggatac aaaaacgtat tcatcggaac cgttgagggc gagccggaag
     1141 agaccgcttg cgagaacgtg atcgaggcag taaaagcagc cggatacaag aaagtaattc
     1201 ttcgtccgct gatggttgtt gcaggtgacc acgcaaacaa cgatatggca ggcgaggatg
     1261 aggattcctg gaagagccag tttgaggcat ccggtgcatt cgatgagatc gacactcaga
     1321 tcgaaggtct tggaagaatc gatgctgttg agcagctgta tgtacagcac accaaggcag
     1381 ctatggacgg caacggcgtt caggacgatg ctgaataatt aggggttgta ttccgaacgt
     1441 aaaatagagt aaaataagat ccggcagtac ggctgaagag actcagtgtg ctgtcggatt
     1501 ttaagttgag cggatgaaaa agatccgctt caaggtgata caaggaggaa atttattatg
     1561 agaaaaaaat tagcagttct ttttcttgga gcagcactga ctgtatccat gctcggcgca
     1621 tgtggcggaa aggatgcagc gtcatcttct gcatctgcag tatctgcgtc ttctgcggca
     1681 gacgcatctt ccgcttctgt acaggatgca tctaaggcag agagcagtac atccacatca
     1741 gatgtttcag cagaaagcgc atcggcagcg gagactgcag gagaaagcag cgcatcagag
     1801 ctggaagacg gtacgtatac ggcagacttc aatacagaca gcaatatgtt ccatgtcaat
     1861 gaagcaaaag aaggaaaggg tgtcctgacg gtaaaagacg gaaaaatgac catccacgtc
     1921 agcctggtat ccaaaaacat tgtcaatgtc tttgtgggaa caaaagaaga agcacaggca
     1981 gacggcgcag agctgatcga gccgaccaca gatgaggtaa cgtattctga cggaaccaca
     2041 gatgaagtgt acggctttga tatcccggta gaaaaactgg acgaggattt caatgttgcg
     2101 atcattggta aaaaaggaac ctggtatgat catgtagtca gcgtatccaa tccggtaccg
     2161 caggagtaat ccgggaaaaa acaaaaagat atccacagaa ggtgaaccat tttgcaccgt
     2221 ctgtccacag aaattgtgga cagagagggg aaaaacggtt ccctttgtgg atatcttttc
     2281 ttatttctcc ggaaacagtt tgcggaacag aaaacggatg aagggtccgc agtatatgag
     2341 ctgccagaag aaggcaaccg gaaaatttaa gaacgtggtc tggagccaga cagcgacaaa
     2401 ttcttttccg gcatttttaa acagcagcgt tgcaataaaa ctcataatcg gacacataat
     2461 agcaataatc ataatggaaa ttgccagcgt gattacgatc ggacgatcct gcggtgttac
     2521 catgcaaaaa gctaatttgt gagccagatg atccacgagg aaaaactcga ggataaaagc
     2581 ggccggccac atgattatca tctcgtgaaa cgccatcaga aagacctgat ttgtcattcc
     2641 gccgatgttc atggaaatgt tgtagcagat catggcatag accatcagaa aggacatcat
     2701 gagggtaaat acaacgtttt gaaattttgt ttttggcatc gattgttctc cttttctttg
     2761 caattttgaa aaatgggcgc aaaaaagaca catgtgttgt tcgttaacca agcaatgcac
     2821 ttgtgccgtt tccaatctga taccaatctt ttctgcagtt tttaattttg aaccgtcgat
     2881 tattataacg tttccggcgg cgtttggcaa gagaaaattg agaaattttt tgaaaagata
     2941 gataaacaag aaaattcgat gcgtgctgga agcacggttt ggcgaatggt gcggggaacg
     3001 gcaccggcgg gaaaaagaat ggattgcgaa acgggagcgg gattgctaca atagaacata
     3061 gtgaaaacaa agagaaaacg caaaaggaaa tcaatattat gtcagagatc agaaaaatcg
     3121 aaaactttgc ggcgccggag ctcgacgtgt atgcgcgtct ttcagaaccg cagttattgc
     3181 actattatga accgcagcca ggcctttttc ttgcggagag tccgcgtgtc atcgagcgtg
     3241 cgctggatgc cggatatgaa ccggtttcgt tccttgccgg aagcgcagag cttgccgcaa
     3301 acgaagcact ctttgcgcac tgcccggacg cgccggtcta tacagcagag acgaaggtgc
     3361 tggaacagct cacaggcttt gccctcacac gcggcatgct ctgcgcgatg caccgccgca
     3421 cgcttccggc tatggaggaa atctgccgga atgcccgccg cgtggcgatt ctggaaaatg
     3481 tcgtgaaccc gacgaatgtc ggtgcgattt tccgttctgc cgccgcgctt ggcatcgatg
     3541 ccgtgctcct cacatcaggc tgcagcaacc cgctgtaccg ccgcgccatc cgcgtgagca
     3601 tgggaaatgt cttccaggtc ccgtggaccg tgctgccagg cggcagttac tggccggagc
     3661 agggaattgc tgcactgcag gaacttggat tttataccgt tgcgatggca ttaaaagaag
     3721 actccgttcc gatggatgat ccgtcgttaa aaacgcatga aaagctcgcc attctgctcg
     3781 gaaccgaggg tgacggactg gcaagtgata cgatcgctga aacagatgca accgtgctga
     3841 ttccgatgac acatggtgtg gattcactga acgtcgctgc ggcgagcgct gtcgcattct
     3901 gggagctggc gggaagaaga taaagatgtt ccgtcggtac cggagattac ggaataaaaa
     3961 taggatcaga gatagatttt cgggatgcat attttgatgc atcccttttt gaatttacga
     4021 attttgaaat tccttccgat acatctgcgg cgtacatccc actttgtttc ggaactgccg
     4081 gttgaagttg gaaaggttgc ggaaaccatt gtcgtacgcg atttcggaca cactccgcgt
     4141 cgtactgcgc agcgcctcgc aggcggcttt gatccgaagc tgtgtcagat attcaacggc
     4201 cccgagtccg aaggtctgct taaaacagct cataaaatag cttttgctca aatgcgacag
     4261 cctggcaagt tcctctatgg taatcgggtc ggcgaagtgc tcctggatat aggcgagcac
     4321 aggccgcagg ttttcagcag ccgcaggcat ggcgggacga tcggtgcaga gttccggcga
     4381 tgcggcgagc agatagaaga agcggagcag ctcacttttc agcagaagat cggagacagc
     4441 agagttttca cggatgcagc ggaaaatctg atggacggat gcagaaagtg cagtataacc
     4501 ggaatgttcg ggtgaaatcg gtgcctggat gcggcgccgg gcggagaaaa acggctgcag
     4561 aatttccgtg tagctgcggt cgtcataccc gccgatcagc atattctgtt ggaaaacaat
     4621 cgtatcataa aacatcgact cctgctcggc gggcagaatg gcatgtacga ggttcggctg
     4681 aatcagaaaa atatcaccgg catgcgccgt aagcagacgg tcctcatact gaaaataccc
     4741 cgttccgcgt ttgatataat tcagctccat ctccccgtgc cagtgcagcg gtacgccggg
     4801 aaaatagtcg ggcatccggc agtcataata actgtaggga acctgcatgg agccgtgctg
     4861 gcgtttttct ttcgcagcag aaagcatcat aaaatcctcc tcccaaaagc atgtccgtaa
     4921 aatgccggaa atggcagagc gtggcatgga taaaatcagt taatgttcac tcagcagtga
     4981 aacacttgct gtttcgctgc gtaagaaagt gattcaggtg tgagcagccc tccttttgcg
     5041 aagcaaaact ttaaatgagg ggtgcgaagg ttacagttcg ctgggtacca gtgaactgta
     5101 actaaaatca tagaatagta ttagaatgaa ataggaaatt actggtattt cagcggatac
     5161 agtagtaata tatcatatag aaacacttgc tgcaacgacg aaaatacagc agaaataaga
     5221 gcgaaagagg aaaagaaaaa atgtcaaaat ggttagataa cgcagtattt tatgagattt
     5281 acccgcagtc cttcaacgac accaacgccg acggaatcgg agatttccag ggtatcattg
     5341 aaaaattaga ctacatcaag gagctgggct gcaacgccat ctggatcaac ccgtgcttcc
     5401 tgtccccatt cggtgatgca ggatacgatg tcgcagatta ctgccgcgtt gcaccgcgct
     5461 acggaacgaa tgaggatctg aagagagttt tcgaagaggc acacaaacgt gacatgcacg
     5521 ttctgctcga cctggttccg ggccatacat ctgttgagca tccatggttc aaagaatcca
     5581 tgaaagcaga ccgcaatgaa tttacagacc gctacgtatg gaccaataac gtatgggaag
     5641 caccggaagg aatgggaagc ctgcgcggca tttccgagcg tgacggcgcc tgcgccatca
     5701 acttcttctc caaccagccg gccctgaact acggctttta tcagccggat ccggaaaaac
     5761 cgtggcagca gtcctttgat gatgagggac cgcaggcaac gctggcagcg atggaagatg
     5821 tcatgagatt ctggctgaac atgggctgcg acggcttccg tgtcgatatg gcgggctctc
     5881 tggtaaagaa cgatcaggaa ggcaaaggaa ccatccgcct gtggcagcag gtacggaaat
     5941 tcctcgacgc cgagttcccg gatgcagcca tggtatccga gtggggcgag ccggacaaat
     6001 ccttacaggg cggcttccac atggacttcc tgcttcactt tggaccgtcc cattacaacg
     6061 atctgttccg ctgcgatgag ccattcttct ccaaacgcgg aaaaggagat atctctgagt
     6121 ttgtagagac ctacaaaacc aactacaata agaccgcaca gaaaggcctg atctgcatcc
     6181 cgtccggaaa ccatgatatg gaccgtctgg cacgccgtct gcagggggat gagctgaaaa
     6241 ttgctttcgc tttccttctt tccatgccag gtgcgccgtt catctactac ggtgatgaaa
     6301 tcggtatgcg ctacgtcgaa ggcctgaaat ccgttgaggg cggatacaac cgcaccggtt
     6361 cccgttctcc gatgcagtgg gatgacagca ccaacgcagg attttccagc gcaccggcca
     6421 gcgacctcta catcaccatg gatccgggca aagaccgtcc gaccgcaaaa gcacagatgg
     6481 ccgatccgga ttccctgtac cacgaagtta aaaaactcat ccagatccgc cagagcagca
     6541 aagcactctt aagccgcgga accatcgagt tcgtttatgc agagaaaaac cagtatccgc
     6601 tggcatacgt ccgtgaggct gagggtgaaa aagtcctcgt catcatcaac ccatccgaca
     6661 aagagcagtc cttcccatac agtgcagagc tgaaggatgc cctctacacg atcggaggaa
     6721 aagcagaatc caaagacgga accatcacca tcccggcaca gtccgcagga ttctatcata
     6781 tctgatcgaa aataaaaatg acagggagat ccaatcgaat tttttcggag gatctccctg
     6841 tttttaaata tgatacactc tcagctcatg caatgccctg gtcgctgcaa tatactgcgc
     6901 ctgcatcatc atctcccgtt tctcgtcctt tgggaaaatc gagaacacct gatcaaactc
     6961 cagccctttc gccagataaa atgtggtaac cgtcagtcct ttcttaaaac tggagctgtc
     7021 acggttcagc caagaaagcc gcttctcaca gtcaaaatct ttttccgcca gaatctcccg
     7081 caaaatcaaa taagcccgtt ccgccgtctt ctcatccggt acaatcaccg ccgccgtctc
     7141 atacgttttc tcctctggaa acagcgtatc cactgcctcc cgcagagccg cctcaagatc
     7201 cgtgacatcc cgttccaaca ccggcattcc gtgccgttca aacagctcca gatccgtcac
     7261 gccaatgaga tttgccgcat acgacgcaat ttccaccgta ttccggtaac tcttccgcat
     7321 ctcaatccgc cgcagatcct tcccgaaaat ccccggtaaa aactgcagta catcctgctg
     7381 ctgatccgcc atcgtctgcg cccggtcgcc gagaatcgtc atcttacagg aaaacatccg
     7441 ccggataatc agatactgga gccgcgaata atcctgcatt tcatcaatca ccagatgctt
     7501 aatattcgac cgctccgcct gccgcgacag gcggtacttc agatacagaa ccggatacac
     7561 atcctcatac cgcagtttcc gcttctccaa cggccgccgc ggcagcgtct gatatccctc
     7621 tttcttcaga aaacgattgt agagaacata aaaatccatc gtctcataca tcttctggaa
     7681 tttttcgatg agcggaatcc gctcctcctc cggcagatcc cggttcagca gcgtctcgat
     7741 ctcgtcaata aacgtctccg ccaccgcatc catccgcgag agcagcggaa tatccgcaaa
     7801 cttgtcataa aacagcgtaa taatttccga ttctgacttc acaaacgact tatacgaaac
     7861 gtctgagaaa ttcatcagct cgtcctcaag ctccagcgca aatccctcca tctggtccac
     7921 aaacgcctgc gactgtttta aagcaaaccg ttctgcggct tttggatccc gcatctcccg
     7981 ctcaatctga tcacagcgat cctcacagtc cgccgccgtg tcctgtaatt tgcggtaagc
     8041 aaagagatca aaactcatct cctgaatatt ctcctcaccg agctccggca gaatatgtga
     8101 aatataatcc gaaaataccc cgttcggcga taaaatcaga atattcgaag acttcagatt
     8161 ctgtctgtca tgatacaaaa ggtaggcaat ccgatgcagc gccaccgatg tcttgccgct
     8221 tcccgccgct ccctgaatta ccagaatccg gtccttcgta ttgcggatga tcgcattctg
     8281 ttccttctga attgtgcgga taatattctt cagctgtacc tcgccgttgc tgccaagctc
     8341 cgcctttaaa atatcatcat caattttcac atcactctca aatccgtaaa tcatctttcc
     8401 atcccggatc ttatactgcc acttggaaca gatttcccct gtcatctcgc cgcccggcgc
     8461 cagataggaa gccggcccgc ggtcaaaatc gtagaaaaga ccgctgaccg ggcttctcca
     8521 gtcgtaaatc agcggcagct cgcccggccg ctccgcaaaa ttgccgatgc cgatatagaa
     8581 aatttccggc tcatcatccc cgtcatagcg gaaatccaca cgcccgaaga acggagaatc
     8641 cagcattttc cggaagcggc tccgcagaag aagctgctcc tgattcgcat tcacctgatg
     8701 tagcagcgcc tgctgattgt cgtaatcctc atatccgtac tggtccatct cggtatagtt
     8761 ctgccagtaa taatcgtgca tattttcaat ttctttctgt ccgtccagca gagactggct
     8821 gatctcggca agacgcgcct gcagcttttc cagcacaaaa gaaagatatt cctgtccgtt
     8881 tttctggtct gtcataaaag gaaacctcct cttaaaaata taatgtcgag tgccggaaaa
     8941 cccggctgat cttatcagta tagcagacag gcacgtttcc tgcaattcaa acataaaata
     9001 aagaaaaatc ccctttgagg tgccgctttt cttggaaccc tttttaaaac cactgtctgc
     9061 acaggaagaa aaaagctgtc tggatgcgta tcagaggggc gatacaaaag ccagagacat
     9121 tctggtcgag cggaatctgc gcctggtcgc gcacgttgtg aaaaagtatc agggcctgcc
     9181 cgatgatctc gacgatctga tttccattgg aacgatcggc ctcatcaagg cggtgatgac
     9241 atatgattcg aaaaaaggaa accggctggc cgcatacgcc gcccgctgta ttgataatga
     9301 gctgctgatg tatcttcgga gcaaaaagaa aacggcacgg gagatttccc tctacgagcc
     9361 gatcggaacc gacaaggagg gaaatgagat ccatctgctc gacatcgtgg agggcggcgg
     9421 ggaggatatc gcggaacagt acaaccggaa agaggacgcg gtgcggctgt actgttatct
     9481 ggattcggaa ttaaaagagc aggaaaaaca ggtgcttctt ctccggtacg gccttggcgg
     9541 aaaagcagaa tacacacaga aacaggtcgc tgcccggctc ggcatcagcc gttcgtacgt
     9601 gtcgcggatc gagaagaatg caattgaaaa gttgcggtta cggtttgccg cttcgtaaat
     9661 gcttgacatt tcaaacggac tgcttctata ataaagggta aggaaatcag gtgcagaaaa
     9721 ggtttcctta tccttacttt ttcgaacaaa aatcaaagat aatatcttcc cggtgttctt
     9781 gccggacatt ttcccaaaat gaaaaataaa taggaactat acgacctgct actgaaagaa
     9841 aaggaggttt tctttgtatt gcagtgtatt atcagccacc gtctcgggca tggagatgat
     9901 tccggtgcag gtggaggcgg atgtcagtga cggcatgccg cagtttacga tggtcggcta
     9961 cgtgagtgcc caggtaaagg aggcgcagga ccgcgtgcgg acagccctca aaaacatggg
    10021 aatttccctg ccgccgaaac gaattacgat taatttatcc ccggcggacg tccgaaaaga
    10081 aggatcgcgt ttcgatcttc cgattgcagc gggcgttctg atgacgctcg gccggattcc
    10141 gcccgggtca ctccggaaaa cgatggtgct cggcgaactc ggccttgacg gccatgtgca
    10201 ggagatcagc ggtgtatttc cggcagcggc gaaggcgcgg gagcttggat gcacaacgct
    10261 tcttgttcca gccggaaatg ccgcggaagg gggtctggtc ggcgggcttc acgtcgttgg
    10321 aatccagaat ttacaggagc ttttgaacta ctgctgtgaa gggaaaatgc cgtcgcttcc
    10381 gtctatagaa aatcaaaaga aggaggacgg aaaagagccg gacttttccg aagtgcaggg
    10441 ccagacggcg gcgcggcggg cggcgctcat cgctgcggcc ggatttcaca atctgctgat
    10501 gctggggccg ccgggcagcg gaaaatccat gatcgcaagg cggattccga cgatcatgcc
    10561 tccgatgtct gaggaggagc agatggaagt aacgcggatc tacagcatcg ccggaatgct
    10621 tgccaaagga gagggtgtcc gccgggagcg gcccttccgt gctccgcatc acacgatgac
    10681 gccgcaggct cttgcaggcg gcggaaaaca gccgtctccg ggcgaaatta cgctggcaca
    10741 ccgcggtgtt ctcttccttg atgaactccc ggagatgacg cgcacaacca ttgagatcct
    10801 gcgccagccg ctcgaggagc accggattgt gatttcccgt gtcagcggaa actatgtgtt
    10861 cccggcgtca tttctgatgg tggcggcaat gaacccctgc ccctgcggct tttaccccga
    10921 cttatcgcgc tgtacctgcg cacccggcga catcagccgg tatctctcga gagtcagcca
    10981 gccgttcctc gaccgcatgg atctctgcgt gcaggtggaa gcactttcct acgaggatct
    11041 gcacggcgag gagcggtgcg aatcctcggc caagatgcgg gaaaaagcga cggcagcggc
    11101 ggaaattcag gcggcgcgct accggcagga gtcgattcat ttcaatagcg aactgaaagc
    11161 aggccagatc gagaaatact gtatcctgga aaaggcagcg gaagagcttt tagaggatgc
    11221 cttccgccgt ctgcgcctca gcgcgcgttc ctaccacggg attatccgca ccgcgcggac
    11281 aattgcggat ctggacgggg cggtcaaaat cggcgtgccg catatcagtg aggcgatctg
    11341 ctttcggagc gccgacaaaa gtatatggag gatctgacgt atggcgatgg aaatctttac
    11401 agtcggccgc gggcaggagg gctatccgga gcggcttatg ccgttcgcgg acatgccgtc
    11461 ccgccttttc gtgcggggcg cgctgccggc ggatgagcag aaaacagcgg cgattgtggg
    11521 cgcgcggata tgtacggcgt acggaaagag ccaggcggcc tttttcgcgc aggtgctggc
    11581 ggcaaacggc gtggcggtga tcagtggact ggcgtgcgga atcgacgcgg cggcgcacga
    11641 gggggcactg cgcggaaagg gaaagacgtt tgccgtgctt ggctgcggcg tggatatctg
    11701 ctatccgaaa cagaattacc cgctgatgcg gcggatgctg gaaaacggag gcggtgtctt
    11761 aagcgaattt ccgccgggcg cagagccgct tccgtggcat ttcccgatcc gcaaccgcgt
    11821 gatcagtgcg ctggcagatg tcgttctggt aattgaggca aaagagaaga gcggctcgct
    11881 cattacggcg gactacgcgc tggagcaggg aaaaacggta tttgccctgc ccggacggac
    11941 aacggatgcg acaagccgcg gctgcaaccg cctgatcgcg cagggagcgg aaattgcgat
    12001 ttcacccgaa acgattctcg aggagctggg cgtgaaaaag gccgggacaa aaagagccgc
    12061 accgcgtccg cgctatggaa aagaggcgga aatggttctg aaaacactcg cggcagaacc
    12121 gcagaatctt gcacagcttg ccggcagcac cggaatcccg ctgcaggagc tgaccggcat
    12181 tctgctggca ctcgaaattg acggcgttat tgcggaaaat ccgccgggcg tgtacatggc
    12241 gttatcgtaa aagggacgag gaaaagaaag gttttgtgat attccgcccg gtgcacaatt
    12301 ataatagaag aaaaagaaaa aagggggctc ttctgcgccg cgtgcatgtc tgaaaaagga
    12361 aaaagaaaag aaaaaaatca ggaaaaatcg ttaaaaaata gaaaaaatcc tgttattttg
    12421 tcgggaaaca tcgaaaaatg gtacatttta aaaatggaaa acacttgcaa gaaaaaactt
    12481 tttggtgtat agtcagtgga agttacgact aatttaggaa ttattctgga atttgtaatg
    12541 atgcagaact atgcgatatg aaaaacgcaa tatagatctg gacagttata aaaatatgta
    12601 ttcaggaggc atcagaggtg gcaaaatatc ttgtaatagt ggaatcaccg gcaaaagtca
    12661 agacggtgaa aaagtatctc ggggcaaatt acgaagttgc agcatctatg ggccatgtgc
    12721 gcgatctgcc gaaaagccgg cttggaattg atgtagaaca tgactttgaa ccaaaatata
    12781 tcaccatccg cggaaaagga gagcttctgg catctcttcg aaaagccgca aaaaaagcag
    12841 ataaagttta tctcgcaact gaccctgacc gcgagggaga ggcaatttcc tggcatctgt
    12901 cgattgcgct gaatctcgat gagaacaaga tgcgccgcat caccttcaac gagatcacaa
    12961 aatcagcagt caaagcgtcg atcaaacagg cacgcgacat cgatatgaac ctggtggacg
    13021 agcagcaggc gcgccgctgc ctcgaccgta tggtaggtta tgaaatcagc ccattgttat
    13081 ggaagaaaat caaacgcgga ctttccgccg gccgtgtgca gtccgttgca ctgcgcatca
    13141 tcggcgaccg cgaggatgag atcaatgcgt tcattccgaa agaatactgg acactggaag
    13201 cagatctgaa aattccgggc gagaagaaac cgctcgtcgc aaaattccac ggaaaaggca
    13261 gtgaaaagat ggcggtcgag acgaaagagc agctcgatga aatcttaaag ggactggaaa
    13321 atgagacctt taccgtatcc gaagtgaaaa aaggagagcg ttccaagaaa gcgccgcttc
    13381 cgttcaccac gagtaccctg cagcaggaag catcgaaggt gctgaacttc tccacgcaga
    13441 agacgatgcg tctggcgcag cagctctacg agggcgtgga agtaaaggga aagggaacga
    13501 tcgctctgat ttcctatctg cgtaccgatt ctacacgaat cagcgaggag gccgatgcgg
    13561 cagcacgttc ctttatccag gagcagtacg gcacaaaata taccgcaagc ggggacacga
    13621 cggagaataa aaacagcaag gcacaggatg cgcacgaggc catccgtccg accgatgtga
    13681 cgaatacacc ggtaatggta aaagaatccc tgccaagaga tctgttccgc ctgtatcagc
    13741 tgatctggaa acgctttgtg gcaagccgca tgcagccggc cgtctatgag acaatttccg
    13801 cacggatcaa tgcaggagag tatctcttta cagcagcttc ctcccgtctg gcatttgatg
    13861 gtttcatgtc cgtttacacc gatccggatg aggaaaagaa agagaaaaac gccgctgtcc
    13921 ggagcctgga ggcaggaacg gagctggcac tggaaaagat ggatccgcag cagcacttca
    13981 cacagccgcc ggcacactac accgaggcat cccttgtcaa aacacttgag gaactcggca
    14041 tcggccgtcc aagtacgtat gcgccgacca tcacaacgat catcaaccgc cgttacgtgg
    14101 caaaggagaa caagaacctt tacatgacag agctcggcga ggtggtcaac aacatgatga
    14161 aagaggcatt cccgagcatc gtggatgtca actttacggc aacgatggaa gctttgctcg
    14221 acagggttgg agatggaagt gttccgtgga agacagtagt cgagaacttc tggccggatc
    14281 tctatgaggc ggtcacagag gcagaaaaga acctcgaaga agttaaggta gaggatgagg
    14341 tgacagatgt catctgcgag aactgcggcc gcaacatggt ggtcaaatac ggcccgcacg
    14401 gaaaattcct cgcctgcccg ggattcccgg actgcaagaa cacgaagccg tatctggaaa
    14461 agatcggtgt tccgtgtccg aagtgcggaa aagaagtgat cctgcgcaag acgaaaaaag
    14521 gacggaaata ttatggatgc gaaaacaatc cggaatgcga tttcatgtcc tggcagaaac
    14581 cgtctgccaa gaaatgcccg cagtgcggcg gctatatgct ggaaaaaggc aataagctgg
    14641 tctgcgcgga cgagcagtgc ggctatgtag aagcacgcga aaagaaagac gaagactgag
    14701 aatgacaaag cggtctgtac tggtgaaaaa ctggtgcagg ccgttttttt tgcttatcga
    14761 ggcggaaaaa caggaagatc cgaaaaaatt tagcatgaat ttttataaaa tttatagttt
    14821 tctgaaaaat atattgattt ccggaaaact acatgataga atgagcttag ttttaagggt
    14881 tgtctgaaaa aggcatcagt gtgcattttt ttcggcggat acgccaaaaa atgcagaaaa
    14941 taaagaaagg agaagaacat gagcgtacaa ctgctggata aaaccagaaa aatcaacaaa
    15001 ctgcttcata acaatcactc cacgaaggtc gtgttcaacg atatctgtga agtcctgatg
    15061 gaaacgttaa gttccaatat cctggtgatc agtaaaaaag gaaaagtgct gggcatcagc
    15121 ctttgcccgg gcgtggaaga aattacggag ctgatcgagg ataaggtggg cggttatgtc
    15181 gatgccgcat taaacgagcg tctcctcggc gtactctcca caaaagagaa cgtcaattta
    15241 cagaccctcg gctttgagcg gcctggtgta gagcgctaca ccgcaattat ctgcccgatc
    15301 gagatcgccg gagaacgcct tggtaccgta tttatgtacc gctgcacccc gcagtacgat
    15361 atcgacgata tcatcgtcag tgagtacggc acaaccgttg tcggactgga aatgatgcgt
    15421 tccgtcaacg aggaaaatgt ggaggaaaac cgcaaggttc aggtggttaa atccgcattc
    15481 aacacgctgt ccttctccga actggaagcc atcatccata tctttgatga actgaacggg
    15541 gatgagggca ttctggttgc aagcaaaatt gcagaccgcg tcggcatcac aagatccgtc
    15601 atcgtaaatg ccctccgcaa attcgagagc gcaggcgtca tcgagtcccg ttccagcggt
    15661 atgaagggaa cgtatatcaa ggtattaaat gattatatct ttgatgaact cgacgagatc
    15721 aaagcaagaa agaacctgaa aggaaagggc gatgcccatc cggacgaggc gaaaaaataa
    15781 aaaagtggaa tgtagaaaag aatcggagcc gtttaagaat ggcgccgttt ctttttgtgc
    15841 aaaaagcatt cggggagcgg aaaaaaatgg tatcattccc gtttttagga ataaactata
    15901 attagattct aaagagagta taaaatatga aaaaattgcc aaaataagaa aaaacgagga
    15961 aataagagaa attaagagtt attacgagag aaaaaaagag aattacaaaa ttcatgcatt
    16021 tgcgaaatga ggtggaaaac actatagtaa gaaccatcaa gtagcactcg aatgaactga
    16081 gtgctaataa ataaaactga atgaaatctt gaaggaggaa acataaatga agttagtacc
    16141 tttaggtgac agagttgtat taaaagag
//
LOCUS       DAOMUF010000027        15901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_748_length_15901_cov_3.891708, whole genome shotgun sequence.
ACCESSION   DAOMUF010000027 DAOMUF010000000
VERSION     DAOMUF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_748_length_15901_cov_3.891708"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..715)
                     /locus_tag="PLN77_03040"
     CDS             complement(44..715)
                     /locus_tag="PLN77_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRM43289.1"
                     /translation="MKTIKKYKHFLPVVVYGIFYLKCFQYLETHITKGYHLIHTPFDD
                     MIPFCEFFVIPYFMWFGYIAWSVLYFGLCNKNRREYYQLIINLGIGMTAFLVISYLYP
                     NGQNLRPTVFPRENIFTDLVRGLYLTDTPTNVFPSIHVYNSVAVAVAVEHSEQLKNRP
                     VFRNVCLGLTVLIVFSTMFLKQHSVSDVCCALVMNAGTCMLLYRPAKAGNALRTREKK
                     AKYRM"
     gene            complement(788..1660)
                     /locus_tag="PLN77_03045"
     CDS             complement(788..1660)
                     /locus_tag="PLN77_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRM43290.1"
                     /translation="MMFWIIFFAVLAVLAAGYFFSILPATSRRQECLKFAEWNYAHRG
                     LWNAAEGVPENSLPAFARAAEQGYAIELDIQITKDGRIVVFHDDTMKRMCGNEGKISD
                     YTYEELQQFHLGDSTEKIPLLREVLQTVDGRVPLLIEIKMPGLSTAVCAGFQKEMEGY
                     TGMFCMESFNSLALRWCRRHMPQTLRGQLSGRFSKDDKVHLILRIMARHLMLNFIGKP
                     DFIAYDHRYADCPGFLIDRMLFRTPAFAWTVKDEHVYNRTRKKFDAAIFEHFHPKDGF
                     QKPMNCFLKNNKAY"
     gene            complement(1657..2604)
                     /locus_tag="PLN77_03050"
     CDS             complement(1657..2604)
                     /locus_tag="PLN77_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRM43291.1"
                     /translation="MYHIIVNKKSRSGRGEKLWPVIEGELERRRTEFRVYPTKYRGSA
                     RETAARLTAKGVWNPDDVLLAIGGDGTANEAVDGITSLSDVKFGYIPTGSGNDFARGA
                     GIPSDPLQALEAVLKGEEKEINIGTALLGSQKRRFLVSTGIGFDAGICHEALASKIKN
                     TLNRLHLGKLTYVMIALRQLFYLEPFTLDAALENGEHRSFENVLFAVTMNFPYEGGGF
                     CFCPEADGTDDLLDVMVVSNLAKWKVSYLLPMALFGRHTGFPEVHMERCSSLSFQTDR
                     AEPVHLDGESGGHRTEERIGLETERLRLLGDWVKEGMER"
     gene            2940..4502
                     /locus_tag="PLN77_03055"
     CDS             2940..4502
                     /locus_tag="PLN77_03055"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM43292.1"
                     /translation="MKLSPAFLAYKLSSAFSVLAPENLPLSGTLELPVLYQTSSAATA
                     HRVFLCTEEQIGSLSSLPEESSSLFLLCTEELPEELPAPVSIAVKSSVSAVFSFLQEL
                     FWTYDCWQKELMQARLDGKSIQHLLNLSLPVFAHPLMVVGMDFSIIAAASEEPSFFPE
                     KVFGSLDATRPFLLSLTESSEYASVRDLDGWFRFPDHVTGLGSLCVNITQHGQSAYRL
                     LLLDTKKDLSDSEGFLLEFLAEMIHHAFLHNVMQKTSPAQSLHTVLLRALDDRMADYI
                     AVSQALDALGWHSRHTYCCLYLQLSDMDQKNLTANSVCAYLENILTGCSAFPHGGGIV
                     AFFNLTLSDFSMTDLMERMVHFVEDSSLNAGFSRCMNGHMNLRRQYIQAKIALQFGTR
                     KYPDKRMHPFNDISFDYILDQATKKLPGYMLCHERLLFLQEHDDSSGSDYMRTLRCYL
                     DNHCNVVQTARELFIHRSTLLYRLEKIKEILESDLSSPDEILYLMLSFRFIDLENEEE
                     NRSGSSQPEKHR"
     gene            complement(4438..4800)
                     /locus_tag="PLN77_03060"
     CDS             complement(4438..4800)
                     /locus_tag="PLN77_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRM43293.1"
                     /translation="MAIALILDLILCNLVLKPLAARPRPCWIDEQVKLLVAAPKDYSF
                     PSGHSAASFAAAVSIFVMHKKEGAAALILACLIAFSRLYLFVHFPTDVLAGIAVGFIC
                     AFLAAKIQNGFLLHSRGR"
     assembly_gap    4918..4927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5062..5718)
                     /locus_tag="PLN77_03065"
     CDS             complement(5062..5718)
                     /locus_tag="PLN77_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRM43294.1"
                     /translation="MRITIFSLGGLLGQYVTNTALLHGAQVTAYVPDAKVIWPRKNLT
                     VIEGSLQNRERVLEALLEADAVISVLTPMRVKHVKEEETPLADGNAMILSAMKQLGKT
                     RFVTVGHVCIHAFGDCEDKYQRMSTKFMQIFWPGVYKDMQKMGQVLARSDLNWTLVRT
                     CPGRDGKVKKVGQNCSISLDGSQFRPGYSREALAEFLYEAAADQQMYSRKMPIFYREG
                     "
     gene            5909..6793
                     /locus_tag="PLN77_03070"
     CDS             5909..6793
                     /locus_tag="PLN77_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM43295.1"
                     /translation="MPYTIVIDAGHGGSDPGAVYEGRREKDDNLSLAIAVGELLSRQG
                     VSVIYTRTTDVYQTPFEKAQMANQADADFFISFHRNSSEEPNQYTGVETLVYDNSGIK
                     QTMAENINGALSELGFRNLGVKARPGLVVLRRTKMPALLIETGFLNNEQDNTLYDEKQ
                     EEIARAIADAVLGTLDLETVTEADRRAAETEAARPSVSTADTVTASASPHPTPPEQEN
                     PSDTPETFYRVQTGLFRIRQNADRMLYDLLDQGYPAFLLAEDGFFKVQVGAYRQLGNA
                     ILMERRLRRDGYSTLITT"
     gene            complement(6861..9089)
                     /gene="pcrA"
                     /locus_tag="PLN77_03075"
     CDS             complement(6861..9089)
                     /gene="pcrA"
                     /locus_tag="PLN77_03075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HRM43296.1"
                     /translation="MNQTETGLNPMQMEAVEYTEGPLLILAGAGSGKTRVLTHRIASL
                     IRDKGVNPWNIMAITFTNKAAGEMRERVDKIVGYGSERIWVSTFHSSCVRILRRHAER
                     LGYANNFAIYDTDDQKSLIKDIMKRLQMDPKLYRDRAIMAAISSAKDEMIEPDQYRID
                     AGIDWKLQKYAAVYAEYQAELKKNNAMDFDDLLVQTVKLFQQFPEVLDYYQEQFRYIM
                     VDEYQDTNTVQFRFVSLLAAKYRNLCVVGDDDQSIYKFRGANIENILSFEHVFPDAKV
                     IKLEQNYRSTQNILNAANEVIANNQGRKAKRLWTENPEGDLIDLRQFQSGFEEAEYVA
                     GEITRGVRTEGKKYRDYAILYRTNAQSRLFEEKLLLANVPYKIVGGVNFYARREIKDL
                     LAYLRVIENPEDDLAVLRIINVPRRGIGAATINKVQDWEAEQGIRFYDALLEAEYVPG
                     LMRSLNKIKSFTSFLQVLRAKADYLSVKELLNEIIEETGYVADLQAEGTEEAAARIEN
                     IDELISKIADYEESAEQPSLGGFLQEVALVSDIDSVDEESDYVLLMTLHSAKGLEFPN
                     VFLAGMEDGIFPSYMTITGDDPSELEEERRLCYVGITRAMQHLTLTCARQRMIRGEVQ
                     YNKMSRFVKEIPRELISLERERGEIEDRKPKMDIPTRGSVYAAMKQAFREKPQQAKTF
                     SAQKATKLDYEIGDTVRHIKFGVGIVTDIVDGGRDYEVTVNFDQAGTKKMFASFAKLK
                     KL"
     gene            9268..9888
                     /locus_tag="PLN77_03080"
     CDS             9268..9888
                     /locus_tag="PLN77_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HRM43297.1"
                     /translation="MTIDSDKILHDLLNSLDSLEGIRPGEIPNIALYMDQVTTFMDAQ
                     LASSRRFESDKVLTKTMINNYAKNKLLPPPVKKKYSPEHMMLLVFIYYFKNILSITDI
                     QTLLAPITDRYFGTESGFSIKDIYDEIFQMEKEEIASVKEDLLKKADVSKSMFGSSPE
                     EDREFLQLFSFICLLSFDVYLKKQMIEHMVDELASLAAPSDPKKKN"
     gene            complement(9947..10246)
                     /locus_tag="PLN77_03085"
     CDS             complement(9947..10246)
                     /locus_tag="PLN77_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HRM43298.1"
                     /translation="MNKKLHSEAVDQLFEAILCLKNKEECYTFFEDVCTINELLSLSQ
                     RFEVAKMLREKRTYLEISEKTGASTATISRVNRSLSYGNDGYEMVFERMEKKENV"
     gene            complement(10343..11230)
                     /locus_tag="PLN77_03090"
     CDS             complement(10343..11230)
                     /locus_tag="PLN77_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM43299.1"
                     /translation="MKKVEHGKTFDRNAANPPAFEIFHNTVTEGYPEHWHTAIEIIMP
                     TRGEYEVVVGKDSYNLKEGDIIMINSGVVHGMRFVSQGERIVMLVEPGCVENQEEMET
                     IFLRLPAIYFKKEDEEDPFYLFLRSQILELVREYDNEGAVMTHRICTGLKELFSEFGR
                     KGFSDANAQNTFGAMKQQEYIEAVLSACRYINVHYMENLSLEEVAAVSGFSKFHFTRI
                     FKQYMNMTFYEYLNSKRVKRAEELLYDKKMSITDVAMNSGFSSLSAFNRTFKTVKACS
                     PSDYRRQRESMVQALLMQG"
     gene            complement(11252..12721)
                     /gene="xylB"
                     /locus_tag="PLN77_03095"
     CDS             complement(11252..12721)
                     /gene="xylB"
                     /locus_tag="PLN77_03095"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HRM43300.1"
                     /translation="MYFIGVDLGTSAVKLLLMDEEGKIINIVSREYPLYFPHPGWSEQ
                     KPEDWWEQSKEGIRELVKDVDKSQVAGISFGGQMHGLVILDENDNVIRPAILWNDGRT
                     AKQTDYLNTVIGKEKLSEYTANIAFAGFTAPKILWVKENEPDNFAKICKIMLPKDYLA
                     YRLSGAFVSDYSDASGMLLMDVKNKCWSKEMAEICGISLEMLPELHESFDAVGTLKPE
                     VAAELGLPETCKVVAGAGDNAAAAVGTGTVGDGMCNVSLGTSGTVFISSSKFGVDSHN
                     ALHAFAHADGHYHLMGCMLSAASCNKWWMDEIIRTKEYTKEQENITKLGENNVFFLPY
                     LMGERSPHNNPDARGTFIGLTMDTTREDMTQAVLEGVAFAIRDSFEVAKSLGIQIERT
                     KICGGGAKSPLWRRMIANVLNIKVDRIESEEGPALGGAMLAAVACGVFSSVEEAAEKI
                     VRVTETIEPEPELVEKYERQYQKFAKIYPTVKDLFTELL"
     gene            complement(12846..14312)
                     /locus_tag="PLN77_03100"
     CDS             complement(12846..14312)
                     /locus_tag="PLN77_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HRM43301.1"
                     /translation="MNNTPEIKLGIVAVSRDCFPNSLSENRRKAVVKAYDGEIYECPI
                     IVENEIDMMNALEDLKKAECDALVVYLGNFGPETEETLLVKYFDGPCMVVAAAEETGD
                     DLVGGRGDAYCGVLNASYNLKLRNLHAYIPEYPVGDAEDVAAMIKDFVPVATAVVAVK
                     NLKIISFGPRPTNFLACNAPIKQLYNLGVEIEENSELDLFEAFNKHAGDERIPAVVED
                     MAKELGEGNLKPEILPKLAQYEITLLDWIEAHKGSRKYVTLTGKCWPAFQTQFGFVPC
                     YVNSRLTKMGIPVSCEVDIYGTLSEFIGTVVSKDTVTLLDINNTVPKDMYESEIKGKF
                     DYTLKDTFMGFHCGNTASTKLAFCSMKYQMIMARTLPEEVTQGTLEGDIVPGDITFYR
                     LQSTSDAKLRGYIAEGEVLPVATRSFGSIGIFAIPEMGRFYRHVLVEKNYPHHGAVAF
                     GHFGKPLFEVFKMLGVEVDEIGYNQPKGVRYPTENPWG"
     gene            complement(14422..15039)
                     /locus_tag="PLN77_03105"
     CDS             complement(14422..15039)
                     /locus_tag="PLN77_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HRM43302.1"
                     /translation="MQILKVTDEAFRQYGKVIKDLDVSDIITAMSETPCPDDVVYEPS
                     IESLEACKSAQSVSDSLYGGMPIQIGYCNGHNHLLNAVEYHRDSEINIAVTDLILILG
                     KEQDITEDHTYDSSKMEAFLIPAGTTIEVYATTLHYAPCNVAASGFKCVVVLPKGTNT
                     DITLEEKHTPEDDLLFARNKWLLSHPDANIAGSVAGIKGENLSVK"
     regulatory      15192..15301
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15367..>15901
                     /locus_tag="PLN77_03110"
     CDS             15367..>15901
                     /locus_tag="PLN77_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRM43303.1"
                     /translation="MSFFVTADGGLTTAGYVGCVVLMVALVVIASVLSSKKSKGKPMD
                     ARKLAFCAMGIALAFVTSYVKVFELPYGGSVTLFSMLFIVLIANWYGAKTGILVGFAY
                     GLLQFIQGPYVLSLFQVCCDYVFAFAALGVAGFFGKKKNGLLIGYIVAVLARGFFHTL
                     GGYLYWMDYMPENFPQSL"
BASE COUNT         3562 a         4262 c         3729 g         4338 t
ORIGIN      
        1 aggcccgcgc actttctggt gcgcgggccg tccgtttccg tttttacatt ctatatttcg
       61 cttttttctc acgtgtccgc agtgcattcc ccgcctttgc cggccggtac agcagcatac
      121 aggttcccgc attcataaca agcgcacagc acacatccga tacggagtgc tgttttaaaa
      181 acatggtcga aaacacaatc agcaccgtca ggcccaggca gacgtttcgg aataccgggc
      241 ggtttttgag ctgctcgctg tgttctacgg caacagctac ggctacggaa ttatatacgt
      301 gaatacttgg gaatacgttg gtcggtgtgt ctgtcaggta aaggcctctg accagatccg
      361 tgaaaatgtt ctcccgtggg aataccgtcg ggcgcaggtt ctggccgttc gggtacagat
      421 aggagatcac gaggaacgcc gtcattccga ttcccaggtt gataatcagc tgataatatt
      481 ctctgcggtt cttgttgcag aggccgaaat aaaggacaga ccatgcaata tatccaaacc
      541 acataaaata aggaatcaca aaaaactcac agaacggaat catatcatca aacggagtat
      601 gaatcagatg atatcctttc gtaatgtgcg tttccagata ttgaaaacat ttcagataga
      661 aaataccata aaccactacc ggaagaaaat gcttatattt ttttattgtc ttcatagttt
      721 ccctccactt ttctgacgag gtttttctct tcaacctgct gaaattatag cacaccgaaa
      781 accgaagtca ataagcttta ttatttttta agaaacaatt catcggtttc tgaaaaccgt
      841 ccttgggatg gaaatgttca aagatcgccg cgtcgaattt tttccgtgtc cggttatata
      901 catgctcatc ttttaccgtc catgcaaatg ccggtgtccg aaacagcata cggtcgatca
      961 ggaagcccgg gcagtcggcg tagcggtggt cgtaggcgat aaaatccggt ttcccgataa
     1021 aattcagcat cagatgacgc gccattatcc gcaggatcag atgcacctta tcatcctttg
     1081 aaaaacggcc ggaaagctgg ccgcgcagcg tctgcggcat gtggcggcgg caccagcgga
     1141 gggccaggct gttgaaggat tccatgcaga acataccggt gtacccctcc atctccttct
     1201 gaaatcccgc acagaccgcc gtggaaagtc ccggcatctt gatttcgatt aaaagcggca
     1261 cgcggccgtc taccgtctgc agtacctcac gaagcagcgg aatcttttcc gtgctgtcgc
     1321 ccagatggaa ctgctggagt tcctcatacg tataatcgct gatttttccc tcgtttccac
     1381 acatccgctt catcgtgtcg tcgtggaaca ccacaatccg gccatccttt gtgatctgaa
     1441 tatccagctc gatggcatat ccctgctccg ccgcgcgggc aaaagccgga agagaattct
     1501 ccggaactcc ctccgccgcg ttccacagac cgcggtgcgc ataattccat tccgcaaatt
     1561 tcagacattc ctgccgtctc gatgtagccg gcagaatgga aaagaaataa cctgctgcca
     1621 ggacggcaag cactgcaaaa aatatgatcc agaacatcat ctttccatcc cttctttcac
     1681 ccagtcgccc aaaaggcgga gacgctccgt ttccagtccg atcctttcct ccgtgcggtg
     1741 tccgcccgat tctccgtcca ggtggaccgg ttccgcgcgg tctgtctgaa aggaaaggct
     1801 gctgcaccgc tccatatgta cctcaggaaa tcccgtgtgg cgtccaaaca gcgccatcgg
     1861 cagaagatag ctgactttcc atttggcaag attggaaacc accatcacgt cgagcagatc
     1921 atctgtcccg tccgcctccg ggcagaagca aaagccgccg ccctcgtacg gaaaattcat
     1981 tgtcactgca aaaagaacat tttcaaacga acggtgttct ccgttttcca gcgctgcatc
     2041 cagtgtgaac ggttcgagat aaaagagctg ccgcagtgca atcatcacat acgtaagttt
     2101 tcccagatgc agccggttca gcgtattctt gatttttgat gccagcgcct cgtggcagat
     2161 gcctgcatca aaaccgatgc cggtgctgac gagaaatctc cgcttttggc tgccaagaag
     2221 cgccgttcca atgttgatct ccttttcctc ccctttcagc acagcttcca gcgcctgcag
     2281 cggatcggag ggaattccgg caccgcgcgc aaaatcgttg ccgcttcccg ttggaatata
     2341 accgaacttt acatccgaca ggctggtgat tccatcgacc gcctcattcg ccgtaccgtc
     2401 tccgccgatc gccagaagga catcatcggg attccatacc cctttcgcgg tcagccgtgc
     2461 tgccgtctcc cgtgcgcttc cgcgatactt tgtcggatat acacgaaatt ccgtcctgcg
     2521 ccgctcaagc tcgccctcaa ttaccggcca cagcttctcg ccgcgccccg accgcgattt
     2581 tttatttact ataatatgat acattaaagt cataccttct tttttcttct gtaaaaaacc
     2641 acttcaaatc acctgaatca tgttcttacg cagcgaaaca gcaagtgttt cactgctgag
     2701 tgaacagtaa ccaaatcagc catattgtag cagttttccc cgcaaaccac aactatcttc
     2761 acaaaatctt aacttgttgc cgccgtaaaa attcccgcac ccctgtcttt tctttcttga
     2821 cagacctttt tgttctgcta aaataaaaag taccgcggtt tcgtccgcag aacgtcactc
     2881 atttctgagg aggtttttat gaatttttac gatatgacac gcgcagggag gatgatacga
     2941 tgaagctctc ccccgcattt ctcgcttata aactaagcag cgctttttcg gttcttgcac
     3001 cggaaaacct gccgctctcc ggcacactgg aactgcctgt tctttatcag acaagctccg
     3061 cagccactgc gcaccgggtc tttctctgca cagaagaaca gatcggctct ctttcctccc
     3121 tccctgaaga aagcagctcc ctttttcttc tctgtaccga agaactgccg gaggaacttc
     3181 cggctccggt ttcgatcgcc gttaagagca gcgtatccgc tgttttttcg ttcctgcagg
     3241 agcttttctg gacgtacgat tgctggcaga aggagctgat gcaggcacgg ctcgacggaa
     3301 aatcgatcca gcatctttta aatctctccc tgcctgtttt tgcccacccg ctgatggtgg
     3361 tcggcatgga tttttccatc atcgccgcag cgtccgaaga accttccttt tttcccgaga
     3421 aggtgtttgg ctcccttgat gcaacgcgcc cgtttctgct ctccttgaca gaaagcagcg
     3481 aatacgcgtc cgtccgcgat ctcgatggat ggttccgctt tccggatcac gtaacgggac
     3541 ttggttccct ctgcgtcaac atcacgcagc atggtcagag cgcctaccgc ctgctgctcc
     3601 tcgacacgaa aaaggatctt tctgattccg aaggctttct tcttgaattt cttgcagaaa
     3661 tgatccatca cgccttcctg cacaatgtga tgcagaaaac ctcgccggcg cagtcgcttc
     3721 acaccgtgct gctccgtgcc ctcgacgacc ggatggccga ctatattgcg gtcagccagg
     3781 ctctcgatgc gctcggctgg cattcccgcc acacctactg ctgcctctat ctgcagctct
     3841 ccgatatgga tcagaaaaac ctgacggcaa actcggtctg cgcctatctg gaaaatattc
     3901 tgaccggctg ctccgcgttt ccgcacggcg gcggtatcgt tgcgttcttt aacctcacgc
     3961 tgtccgattt ttccatgacc gatctgatgg aacgcatggt gcactttgtg gaggacagtt
     4021 ccttaaatgc cggcttttcg cgctgcatga acggccacat gaatctgcgc cgccagtata
     4081 tccaggcaaa aatcgccctg cagttcggca cgcgaaaata cccggacaag cggatgcatc
     4141 cgttcaacga tatctcgttt gactacatcc tcgatcaggc aacaaaaaag ctcccaggct
     4201 acatgctgtg ccatgagaga cttctctttc tgcaggagca cgacgacagc tccggcagcg
     4261 actatatgcg gacgctccgc tgctatctgg acaaccactg caatgtcgtc cagacggcgc
     4321 gtgagctttt tatccaccgc agtaccctgc tctaccgcct ggaaaagatc aaggaaattt
     4381 tagaatctga tctttccagc ccggatgaaa ttctgtatct gatgctttcg ttccgtttta
     4441 tcgacctcga gaatgaagaa gaaaaccgtt ctggatcttc gcagccagaa aagcacagat
     4501 aaaaccaacc gcaattcccg ctaggacatc ggtcgggaaa tgcacaaaca gatacagtct
     4561 ggaaaatgca atcaggcagg ccagaatcag tgcggcagct ccttcttttt tgtgcatcac
     4621 aaaaatagag actgccgccg caaacgaagc tgccgaatgg cccgatggga aggaatagtc
     4681 cttcggagcg gcaaccagca gctttacctg ctcgtcaatc cagcacggac gcgggcgcgc
     4741 tgccagcggc tttaacacca gattacataa aatcaaatct aaaatcagcg cgatcgccat
     4801 tcttacaccg catttccggt atttcggaag gattaagaaa acggcggtca gcgcaatcca
     4861 gatccatctt cacaatgcag tgcatataaa attgaaaaat ccattcttac ccctctcnnn
     4921 nnnnnnnaag gattaagaaa acggcggtca gcgcaatcca gatccatccc gcatttccaa
     4981 gtgaagtaat tcccaccatc acccgatcga gcactgcatt gtgaagatgc tgcagtgcat
     5041 ataaaattga aaaatccatt cttacccctc tctgtaaaaa atgggcattt ttctgctgta
     5101 catctgctga tcggcggccg cctcgtacag aaattccgcc agggcctctc tcgagtatcc
     5161 aggccggaac tggctgccgt ccagcgaaat actgcagttc tgccctacct ttttcacttt
     5221 cccgtctctt cccggacacg tccgcaccag tgtccagttc agatcgctcc gtgccagaac
     5281 ctgtcccatc ttctgcatat ctttatagac acctggccag aaaatctgca tgaatttcgt
     5341 actcattctc tggtatttgt cctcgcagtc tccaaacgca tgaatgcaga catggcctac
     5401 cgtcacaaac cgcgtttttc caagctgctt catggccgag agaatcattg cgtttccgtc
     5461 agccagcggt gtctcctctt cttttacatg tttcacacgc atcggagtca gcacgctgat
     5521 gactgcatcc gcttccagca gtgcctccag cacgcgttcc cggttctgaa ggcttccctc
     5581 aatcacggtc agattttttc tcggccagat cacttttgca tccggcacgt aggcggtcac
     5641 ctgtgcgccg tgaagaagcg ccgtgttggt cacatactgc ccaagcagtc cccccagact
     5701 gaatattgta attctcatga aatcctcact ctcatctgtg tgtcttttgt atcctccgca
     5761 gattttttga cacactttcc taatgttatc tcctattcta acacaccgcc cgtatacgat
     5821 acaatgaaat aaaactaaaa aaagtataac ctttttcgtc ctttcttcat atcgtaagta
     5881 gaaccgtatt ttcaggagct ttgatcttat gccctatact atcgtaattg atgccggaca
     5941 cgggggaagt gatccgggcg ctgtctatga gggacgcagg gaaaaggatg acaatctttc
     6001 cctcgccatc gcggtcggcg agcttctttc ccggcagggt gtctctgtca tttatacccg
     6061 cacaaccgat gtttatcaga cgccgtttga aaaggcgcag atggccaatc aggcggatgc
     6121 cgactttttt atttcttttc accggaattc ttcggaggaa ccgaaccagt ataccggcgt
     6181 ggaaacgctt gtctatgaca actccggcat caaacagaca atggcagaaa acattaacgg
     6241 cgcgctctcg gagctgggat tccggaatct cggtgtcaag gcacggccag ggcttgttgt
     6301 tctgcggcgg acgaaaatgc cggctcttct gattgaaacc ggctttctga acaatgagca
     6361 ggacaatacg ctctacgatg aaaagcagga agaaatcgcc cgcgccatcg cggatgcagt
     6421 gcttggaacg ctggatctcg agacggtcac ggaggctgac cggcgcgctg cggaaacgga
     6481 agccgcccgt ccgtccgttt caacagcgga taccgtcact gcttccgctt cgccccatcc
     6541 cacgccgccg gaacaggaaa atccttccga cacaccggaa accttctacc gtgtccagac
     6601 cggccttttc cgcatccgcc agaatgccga ccgtatgctt tacgaccttc tcgaccaggg
     6661 ctatcccgct tttcttctcg ccgaggacgg ctttttcaag gtccaggtcg gcgcttaccg
     6721 ccagctcgga aatgccattc tgatggaacg ccgcctgcgc cgcgacggct attccaccct
     6781 gattaccacc tgagcgtctg acgcaccagt cgaaaaaaga gcgtgccgga attttgtttt
     6841 ccgacacgct ctcgttctcc ttataacttt ttcagttttg caaacgaagc aaacattttc
     6901 ttcgtaccag cctggtcgaa gttgacggta acttcataat ctcttccgcc gtccacgata
     6961 tccgtgacaa tgccgacacc gaacttgata tggcgcaccg tatccccgat ttcgtaatcg
     7021 agtttcgtcg ccttctgcgc gctgaaggtt ttcgcctgct gcggcttctc gcggaatgcc
     7081 tgcttcatcg ccgcatagac gctgcctctc gtcggaatgt ccatcttcgg cttccggtct
     7141 tcgatctccc cgcgctcccg ctccagggaa atcagctccc gcggaatctc tttgacaaaa
     7201 cgggacatct tgttgtactg cacttcgccg cggatcattc tctggcgcgc acacgtcaat
     7261 gtcagatgct gcatcgcacg cgtgatgccc acgtagcaga gacggcgctc ctcctccagc
     7321 tccgacggat catccccggt gatcgtcatg tagcttggga aaatgccatc ctccatgccg
     7381 gcaaggaata cgttcgggaa ctccagtcct tttgcggaat gcagtgtcat caacagcacg
     7441 taatcgctct cctcatccac cgagtcgata tcagacacca gcgcaacctc ctgcagaaaa
     7501 ccaccgagag atggctgttc ggcgctctcc tcataatccg caatctttga aatcagctcg
     7561 tcgatgttct cgatgcgggc tgccgcttct tcggttccct ccgcctgcag atctgctaca
     7621 tagccggtct cctcgatgat ttcattcagc agctctttga ccgacagata atctgctttc
     7681 gcgcggagta cctgtaaaaa ggacgtaaag gatttaatct tatttaaaga ccgcatcagc
     7741 cccggcacat actcggcctc gagaagcgca tcgtagaaac ggatgccctg ctctgcctcc
     7801 cagtcctgca ctttgttgat cgtcgccgct ccgataccgc gccgcggaac gttgatgata
     7861 cgcagtaccg caaggtcatc ctccgggttc tcgatcacac gcagatacgc cagcagatcc
     7921 ttgatctcgc gtctcgcata gaagttgaca ccgcccacga ttttatatgg aacatttgca
     7981 agcagcagtt tctcctcaaa aagacgggac tgtgcgttgg tacggtagag aattgcataa
     8041 tcacgatact tctttccctc cgtgcggacg ccgcgcgtga tttctcctgc cacgtactcc
     8101 gcctcctcaa aaccgctctg gaactgccgc agatcgatca ggtcaccctc cgggttctct
     8161 gtccacaggc gttttgcttt tcgtccctgg ttattcgcaa tcacttcatt tgccgcattc
     8221 agaatattct gcgtggaacg gtaattctgc tccagtttaa tgaccttcgc atccgggaat
     8281 acatgctcaa aactcagaat attttcaata ttcgccccgc ggaatttgta gatcgactgg
     8341 tcatcatcgc cgacaacgca gagattgcgg tattttgccg ccagaaggct cacaaagcgg
     8401 aactgaaccg tgttggtatc ctgatactca tcgaccatga tatagcggaa ctgctcctga
     8461 taatagtcga gcacttccgg gaactgctgg aataatttta cggtctgtac cagcagatca
     8521 tcgaagtcca tcgcattgtt tttctttaac tctgcctgat actccgcata aactgccgca
     8581 tacttctgca gcttccagtc gattcccgcg tcaatccggt actggtccgg ctcgatcatc
     8641 tcatcctttg cggacgaaat tgccgccatg atcgcacggt cgcggtacag tttcggatcc
     8701 atctggagcc gtttcatgat gtccttgatc agcgatttct ggtcatcggt atcgtaaatt
     8761 gcaaaattgt tggcgtatcc cagacgttca gcatggcggc gcagaatccg cacgcagctg
     8821 gaatggaagg tgctgaccca gatccgctcc gatccgtatc ccacgatttt atcgacacgc
     8881 tcacgcattt ctccggcggc cttgttcgta aaggtgatcg ccataatatt ccacggattg
     8941 acgcctttgt cccggatcag ggaggcgatg cggtgcgtca gtaccctcgt ctttcccgaa
     9001 ccggcacctg caaggatcag aagcggccct tcggtgtact ccacagcttc catctgcatc
     9061 gggtttaatc ctgtttctgt ctgattcatg ttctttctcc ttcatctgct tcgaatcttt
     9121 tcacatgcat ctcagtatat aacaaatccg ccccggttac aagtaagaac gaacatacgt
     9181 tgcattttta tcatggaaac ttttttcgca aagtccttgc tatctggtat taaaaactat
     9241 ataatgggaa cagtgaggtg ataacaaatg acgatagact cagataaaat tcttcatgat
     9301 ttactgaatt cactggattc tctcgaaggc atccgcccgg gggaaatccc gaacattgct
     9361 ttatatatgg accaggtcac gacctttatg gacgcacagc tcgcttcttc gcgccgcttc
     9421 gagtccgata aggttctgac aaaaacaatg atcaacaact atgcaaaaaa caagctgctc
     9481 ccgccgccgg tcaaaaagaa atattcgccg gagcacatga tgcttctggt ttttatttac
     9541 tactttaaaa acatcctctc gatcacggat atccagacac tgctcgcccc gatcaccgat
     9601 cggtatttcg gcacggagag cggcttttcc atcaaagata tctacgacga aattttccag
     9661 atggaaaaag aagaaattgc atccgtaaaa gaagatcttc taaaaaaagc ggatgtatcc
     9721 aagtccatgt tcggcagctc accggaggag gaccgggaat ttctccagct cttttcgttt
     9781 atctgtctgc tgagtttcga tgtctatctg aaaaaacaga tgatcgagca catggtggat
     9841 gaactggcat ccctcgcggc gccttctgat ccgaaaaaga aaaattaaga acaaacgtgt
     9901 aattaaaaaa gacggctgcg ttggaatcgc accgtctttt ttgattttac acattttctt
     9961 tcttttccat ccgttcaaac accatctcat atccgtcgtt tccatagctt aaggaacggt
    10021 tcacgcggct gatcgtcgcc gtggatgctc ccgtcttttc ggagatttcc agatacgttc
    10081 tcttctcccg cagcattttc gccacttcaa accgctgcga cagcgaaagc agttcattga
    10141 tggtgcatac atcctcaaaa aaagtatagc attcttcctt attcttcaga cacaaaattg
    10201 cctcaaaaag ctggtctact gcttctgaat gaagtttctt gttcatttca agccctcctg
    10261 tacgaaaatt aagtccccat ctcattctta taactcggaa tactaccatt ttagcacagt
    10321 aaagtgtgaa atacaagtcc ttttatccct gcatcagcaa agcctgtacc atcgactccc
    10381 gctgtctgcg gtaatcgctc ggcgaacagg cctttaccgt cttgaaggta cggttgaagg
    10441 cacttaaaga agagaatccg gaattcatcg ccacatccgt gatactcatc tttttgtcgt
    10501 acagaagctc ctctgcgcgt ttgacgcgtt tagaattgag gtactcatag aatgtcatgt
    10561 tcatgtactg cttgaaaatt ctggtaaagt ggaacttgga aaatccgctg accgcagcta
    10621 cttcctcgag agaaagattc tccatgtaat ggacattgat ataacggcag gcgcttaaaa
    10681 cagcctctat atattcctgc tgcttcattg ccccgaacgt attctgcgcg ttggcatccg
    10741 aaaagccctt gcggccaaac tctgaaaaaa gctctttgag gccggtgcag attctgtgcg
    10801 tcatcactgc gccctcatta tcgtattccc gcaccagttc cagaatctgg ctgcgtagaa
    10861 acagatagaa gggatcttcc tcatcctctt ttttgaagta gatggccgga agccgcagga
    10921 aaatcgtttc catttcttcc tgattctcca cacagcccgg ttccaccagc atgacaatcc
    10981 gttctccctg ggatacgaat ctcattccgt gtacaacgcc ggaattgatc ataatgatgt
    11041 cgccctcttt gagattatag ctgtcttttc cgacaacaac ctcatattcg cctctggtcg
    11101 gcatgataat ttcaattgcg gtatgccagt gctccggata gccttctgtc acggtgttgt
    11161 gaaagatttc aaacgccggc gggtttgctg cattccggtc aaatgttttc ccatgttcca
    11221 ctttcttcat cttatttctt acctcttttt attacagcag ctctgtaaac agatctttta
    11281 cagtcggata aatctttgca aatttctgat actggcgctc atatttctca accagttccg
    11341 gttccggctc aatggtctct gtcacacgaa caattttttc tgccgcctct tctacggatg
    11401 aaaagacacc gcaggcaacg gctgccagca tggctccgcc aagtgccggg ccttcctcgg
    11461 actcaatacg gtccactttg atgttcagca cgtttgcgat catgcgtctc cacagcgggc
    11521 tctttgcacc gcctccgcag attttcgttc tctcgatctg gatgccgagg gattttgcta
    11581 cctcaaagga atcgcggatc gcaaaggcaa cgccctcgag aacagcctgg gtcatatcct
    11641 ctcttgtggt gtccatggtc aggccgatga aggttcctct tgcatccggg ttgttgtgcg
    11701 gggaacgctc gcccatcaga tacggcagga agaagacatt attctcgcca agttttgtga
    11761 tgttctcctg ctcttttgta tattcttttg tgcggatgat ttcatccatc caccatttgt
    11821 tgcaggaagc tgcactcagc atgcatccca tcaggtgata gtggccgtct gcgtgtgcga
    11881 atgcgtgcag ggcattgtgg ctgtctacgc cgaatttgct gctggaaatg aatacggttc
    11941 cgcttgtacc aagggaaacg ttgcacatgc cgtcgcctac ggttccggtt ccgactgcgg
    12001 ctgctgcgtt gtctccggct cctgctacaa ctttacaggt ctccggcagt ccaagttctg
    12061 ctgctacttc cggttttaag gttccgacag cgtcaaagct ctcatggagc tctggcagca
    12121 tctccaggga aataccgcag atttctgcca tctctttgga ccagcatttg ttcttgacat
    12181 ccatcagaag catgcccgat gcatcggagt agtcgcttac gaatgcgccg gagagacggt
    12241 atgccaggta atcttttgga agcatgattt tgcagatttt tgcgaagtta tccggctcat
    12301 tttctttcac ccagagaatc ttcggtgcgg taaagcctgc aaatgcaatg tttgcggtgt
    12361 actcggagag tttttctttt ccgattacgg tgttcagata atcagtctgt tttgcggtac
    12421 ggccgtcgtt ccagaggatc gccgggcgga tgacgttatc gttctcatcc aggatgacaa
    12481 ggccatgcat ctggccgccg aagctgatgc cggctacctg ggatttgtct acgtctttta
    12541 ccagctcgcg gattccctct ttgctctgct cccaccagtc ttccggtttc tgctcagacc
    12601 agcctggatg cgggaaatac agcggatact ctctcgatac aatatttata attttgcctt
    12661 cctcgtccat cagaagcagt ttgacagcgg aagtaccaag gtcaacacca ataaaataca
    12721 tgtcgaatct cctttttcta ctctttttca aaaagggcgg cagcagacct agatctctgc
    12781 tccgcccttt tgcttatgcg ttactgcata tctttcttat ttttcagtga atcttccgga
    12841 acggattagc cccacggatt ctctgtcggg taacgaacac ccttcggctg gttgtagccg
    12901 atctcatcta cttcaacacc gagcattttg aatacttcga ataacggttt tccgaagtgt
    12961 ccgaatgcta ctgcgccgtg atgcggatag tttttctcta ccagtacgtg acggtagaat
    13021 ctgcccatct ccggaatagc gaagataccg atgcttccga aggaacgtgt tgctacagga
    13081 agaacctcgc cctctgcgat gtatccgcgc agttttgcgt cagatgtgct ctgcagacga
    13141 tagaaggtga tgtctccagg aacgatatct ccctcgaggg taccctgtgt tacttcttcc
    13201 ggaagagttc ttgccatgat catctgatat ttcatagagc agaatgccag tttggtggaa
    13261 gcggtatttc cgcagtggaa tcccatgaag gtatctttta aggtgtaatc aaatttgcct
    13321 ttgatttcgc tctcatacat atccttcgga acggtgttgt tgatgtcgag gagtgttacg
    13381 gtgtctttgg atacaacggt tccgatgaac tcggacagtg ttccgtagat atctacctca
    13441 caggataccg ggatgcccat cttggtcaga cggctgttga catagcaagg aacgaatccg
    13501 aactgtgtct ggaatgccgg ccagcatttt ccggtcaggg ttacgtattt tctggagcct
    13561 ttgtgtgctt cgatccagtc taacagggtg atctcgtact gagccagttt cggcaggatt
    13621 tccggtttca ggtttccttc gccgagctct tttgccatat cttctacgac tgccgggatt
    13681 ctctcgtcgc ctgcgtgttt gttgaatgct tcaaacaggt caagctcgga gttctcttcg
    13741 atttctacgc cgaggttgta cagctgtttg atcggtgcgt tacatgccag gaagttggtc
    13801 ggacgcggtc cgaagctgat gattttcagg ttcttaacag ctacaactgc agttgcaacc
    13861 ggtacgaaat ctttgatcat ggctgctacg tcttctgcat cgccaaccgg gtactccgga
    13921 atgtatgcgt gcaggttgcg cagtttcagg ttgtagcttg cgttcagaac gccgcagtat
    13981 gcatcgccgc gtccgcctac aaggtcgtcg ccggtctctt ctgctgctgc tactaccatg
    14041 caaggtccgt cgaagtattt tacaagcagg gtttcctcgg tctccggtcc gaagtttcca
    14101 aggtatacaa caagtgcatc acactctgct tttttcaggt cttcgagtgc gttcatcatg
    14161 tcgatttcat tctcgacgat gattggacat tcgtagattt ctccgtcgta tgcttttacg
    14221 actgcttttc ttctgttctc ggaaaggctg ttcgggaagc agtcacggct tactgcaacg
    14281 atacctaatt tgatttccgg tgtattgttg atcatttttg aagatcctcc tttttttggt
    14341 tttcttggtt tttggtttct ttttatgttc agaactgctc ttttggggca gtgtttctga
    14401 tttctggttt tgtctctggt tttattttac cgagaggttt tctcctttga tgcctgctac
    14461 gcttccagcg atgtttgcat ccgggtggct taagagccat ttgtttctgg cgaagagcag
    14521 gtcgtcttcc ggggtgtgtt tttcttccag ggtgatgtcg gtattggttc cttttggaag
    14581 gactacgacg catttgaagc cgctggctgc cacgttgcat ggtgcgtagt ggagggtggt
    14641 tgcgtagact tcaatggtgg ttccggccgg gattaagaag gcctccattt tggatgagtc
    14701 gtaggtgtgg tcttcggtga tgtcctgttc ttttccgagg atcaggatca ggtcggttac
    14761 ggcgatgttg atttcgctgt cgcggtggta ttctacggcg ttcagtaaat ggttgtggcc
    14821 gttgcagtag ccgatctgga tcggcatgcc gccgtagagg ctgtcggata cggactgggc
    14881 gcttttgcag gcttcgaggc tttcaatgga aggctcgtag actacgtcgt cgggacatgg
    14941 ggtctcggac atggcggtga tgatgtcgct gacgtcgagg tctttgatga ctttgccgta
    15001 ctggcggaat gcttcgtctg ttactttcag aatctgcatt gtgttggtgc tcctttcgta
    15061 caattagttt aatgattgaa ctaattgtaa tatagcactt gtatgtactt cttgtcaaca
    15121 tgtatttgga tgaaaaaacg gacgggttga caaataaggg ggaggttggt atactttaat
    15181 acgttaaaat aataagacgc taggggagcc tttggctgag aacgtgggac agggataagc
    15241 tcacggaccc tcactacctg aacgggataa ttcctgcgta gggaagctgg aacgtggttt
    15301 cggatgtgat tcgggactgt gtttttatgt gagcccctcc aagcggaatg cgaaggaggt
    15361 ttttttatga gtttttttgt tactgctgac ggcggactga cgacggccgg ttatgttgga
    15421 tgcgtggtgc tgatggtggc gctggtggtg attgcttcgg ttctctcttc taagaagagc
    15481 aaaggaaagc cgatggatgc cagaaagctg gctttctgtg caatgggtat tgcgctggcg
    15541 tttgtgactt cgtatgtgaa ggtgtttgag ctgccgtacg gcggatcggt tacgttgttt
    15601 tcgatgctgt ttattgtgct gattgcgaac tggtatgggg cgaagacggg tattctcgtt
    15661 ggttttgctt atggactgct gcagtttatc caggggccgt atgtgctttc tctgttccag
    15721 gtgtgctgtg attatgtgtt tgcgtttgcg gcgctgggtg ttgccggatt ctttgggaag
    15781 aagaaaaacg ggctgctgat tggatatatt gtagctgtgc tggcgagagg atttttccat
    15841 acgttaggcg gttatctgta ctggatggac tatatgccgg agaatttccc gcagtctttg
    15901 a
//
LOCUS       DAOMUF010000028        15651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_768_length_15651_cov_2.861118, whole genome shotgun sequence.
ACCESSION   DAOMUF010000028 DAOMUF010000000
VERSION     DAOMUF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 15651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15651
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_768_length_15651_cov_2.861118"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLN77_03115"
     CDS             complement(<1..283)
                     /locus_tag="PLN77_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRM43304.1"
                     /translation="MKITILTVGKIKEKYLRDAIAEYSKRLSRYAKLEIIEVADEKTP
                     DNASETVETNIKNKEAERLLKYIRDDAYLITLEIKGKQLTSEELAQKIDT"
     gene            complement(389..1783)
                     /gene="gltA"
                     /locus_tag="PLN77_03120"
     CDS             complement(389..1783)
                     /gene="gltA"
                     /locus_tag="PLN77_03120"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRM43305.1"
                     /translation="MAKGLNRVPVREQEPKVRATNFDEVCLGYNKDEAVEEAQRCLGC
                     KNAKCITGCPVNINIPAFIAQVKEGNFEEAYKIIGESSALPAVCGRVCPQESQCEGKC
                     VRGIKGDAVSIGKLERFVADWAKENGIKPVPAAEKNGHKIAVIGSGPAGLTCAGDLAK
                     LGYDVTIFEALHKAGGVLSYGIPEFRLPKDKVVAAEIENVKSLGVKIETNVIIGKSVT
                     IDELLKDEGFEAVFIGSGAGLPMFMGIPGENANGVFSANEFLTRNNLMKAFRDDYDTP
                     IAHGKKVAVVGGGNVAMDAARTALRLGAEVHVIYRRSEAELPARAEEVHHAKEEGIIF
                     DLLTNPVEILTDDKDWVTGIRCIRMELGEPDASGRRRPVEIPGSEFEIEVDEVIMSLG
                     TSPNPLISSTTYGLDVNRRKCIVAEEETGKTSKEGVYAGGDAVTGAATVILAMGAGKA
                     GAKGIDEYIKSKNA"
     gene            complement(1783..2670)
                     /locus_tag="PLN77_03125"
     CDS             complement(1783..2670)
                     /locus_tag="PLN77_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRM43306.1"
                     /translation="MYKIQEAEMLANNIYRMVVEAPRVAQHCLPGQFVIVKADEEGER
                     IPLTICDYDREAGTITIVFMPIGESTKKMKDLKAGDAFMDFVGPLGQPSEFCSEDIEE
                     LKKKRIVFVAGGVGTAPVYPQLKWLHEHGITADAIVGAKTKDLVILEKEMSAVSNLYI
                     TTDDGSYVRKGMGTDVLRDLVQNQGKQYDVCVAIGPMIMMKFVCLLTKELNIPTVVSM
                     NPIMVDGTGMCGACRLMVGNEVKFACVDGPEFDGHLVDFDLAMKRQQMYKTEEGRALL
                     RYQEGATHHGGCGQCGGDK"
     gene            complement(2794..5334)
                     /locus_tag="PLN77_03130"
     CDS             complement(2794..5334)
                     /locus_tag="PLN77_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRM43307.1"
                     /translation="MANTTKGSGKTKKKTSASGTAKKKQTQTTARKTTVKKSSTAKRT
                     SGTRKNTVREPMDEGVRAEIILLSVLAVSILLMLSNFGMGGVIGQAVCDFFFGLFGVT
                     EWAAPVLIFFGTAFYIANRQNFLIVRKTGGAISCFVFFCAFMQLLASGYTRDTTFLDY
                     YFDSAYDHLGGGILGGSVVKILGMGFGQIGAWVIIVIAIVISVIVMTQKPILAPIHEK
                     TNDHRRMAEERRQERLARQAAMEEEEGKELILEPVEEPKKAGKKKKSRLLSHVESKAD
                     LNENDDTHQMEMPELSIHRGSVPAEEPEKTEPEMTRQAAEEAKKEKTSADHTRNPRSS
                     YEEIEADVDGIRQEIGKKAEEKRPEYKTPPMNLLKKGTRGAMGDSDAHLREVARKLEE
                     TLDSFGVKVTVNNVSCGPTVTRYELMPEQGVKVSRIVGLTDDIKLSLAAADVRIEAPI
                     PGKSAVGIEVPNKTNTAVMLRDLLETPEFKNFSSNLAFAVGKDIAGQPVIADIAKMPH
                     LLIAGATGSGKSVCINTLIMSIIYKAKPEDVKLIMIDPKVVELSVYNGIPHLFIPVVT
                     DPKKASGALNWAVAEMTDRYNKFAEFNVRDMKGYNAKVAELPPDENGKKPEKLPQIVI
                     IVDELADLMMVAPGEVEDAICRLAQLARAAGIHLIIATQRPSVNVITGLIKANMPSRI
                     AFSVSSSVDSRTILDMGGAEKLLGKGDMLYKPQDYQKPARLQGSFVSDKEVSDVVAYL
                     KDHYGENAYDPDIEKRIHTVSLDGGSAAGGGDNRDNYFVEAGKFIIEKDKASIGMLQR
                     VLKIGFNRAARIMDQLADAGVVGPEEGTKPRKVLMSMEQFENYLEEHG"
     gene            complement(5359..6225)
                     /locus_tag="PLN77_03135"
     CDS             complement(5359..6225)
                     /locus_tag="PLN77_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRM43308.1"
                     /translation="MTVIEALFLGLIQGITEFLPISSSAHLIIFRHFMKIEDTQTVFF
                     NVLLHFGSMIAILLTFSKEWKRLFLALCGIIIDLFHNLKEHFSSQHQERKQYRKVLGS
                     NYRMLAFLLILTTIPTAVIGGMIQSLVTLTSSNLLAPGVGLYLTAILLVVADMLPEGE
                     KTQKNLKPKDALIAGFFQGFSTIPGLSRLGSTISACVIGGMTRSYAVKYSLIVSVPAI
                     IGATIVEIATMGKNKIVFHPVYLVGVFMAALAGYFTIRILMRVVKKRKLKYFALYCLL
                     VGTFSIAGYFLL"
     gene            complement(6347..7006)
                     /locus_tag="PLN77_03140"
     CDS             complement(6347..7006)
                     /locus_tag="PLN77_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRM43309.1"
                     /translation="MAESQKNQTEKENIVELKTNIYLLSIIGEVEGHESLGERTKATR
                     YEHILPALARAEEDKSIDGLLILLNTVGGDVEAGLAIAEMIASMRKPAVSLVLGGGHS
                     IGVPLAVAADYSLIVPSATMVIHPVRTNGMFIGVAQTYRNMEKTQDRITGFVSAHSKI
                     TKERLEELMLDTKMLVKDVGTMLDGPQAVEEGLIDEVGGIARALEKLNELMKKAAGTE
                     N"
     gene            complement(7090..7818)
                     /locus_tag="PLN77_03145"
     CDS             complement(7090..7818)
                     /locus_tag="PLN77_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM43310.1"
                     /translation="MSGHSKFANIKHKKEKNDAKKGKIFTIIGREIVIAVKEGGPDPA
                     NNSKLRDVIAKAKANNMPNDTIDRGIKKAAGDANADNYERITYEGYGPNGIAIIVETL
                     TDNKNRTAANVRSAFTKGNGSVGTPGCVSYMFDKKGQIIIDKEECEMDADDLMMLALD
                     AGAEDFADEEDSYEIITDPDDFSAVREALEKDGIAMAEADVTMIPQTYVELTDPQALK
                     DLNRTLDLLDDDDDVQYVYTNLDE"
     gene            complement(7956..8303)
                     /locus_tag="PLN77_03150"
     CDS             complement(7956..8303)
                     /locus_tag="PLN77_03150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM43311.1"
                     /translation="MVIKRGDVYYADLRPVVGSEQGGVRPVLIIQNDIGNRHSPTVIC
                     AAITSRMNKAKLPTHIEIEASAYGIVRDSVILLEQLRTIDKRRLKDKICHLTPEQLAL
                     VNEALKISLDLPT"
     gene            complement(8496..10004)
                     /locus_tag="PLN77_03155"
     CDS             complement(8496..10004)
                     /locus_tag="PLN77_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HRM43312.1"
                     /translation="MKRILTAAQMKQADRNTIETMGVPSLVLMERAALSCVEELQNGT
                     WDTGKVLAVCGPGNNGGDGAAIARILKTKGVDAELFCLGNPEKYSEGMRAQKKIAENY
                     GVREVKNPDFREYTVIIDAIFGIGVSRPLAGEYRRAVEAICASGVPVLAVDIPSGIHT
                     DTGEVLDAAVKARATVTFACAKPGLLFDPGKRYAGEVLVRDIGIGFDAGEEETPWYGS
                     VEKEDLDRFLTRTPMGNKGTFGKVLVLAGSGAMCGAAVLCARAVLASGAGMVKVVTEE
                     RNRTPLFCALPEAMADFWKEDEPLPEEALLQDLAWADAVVAGPGLSKSRTAKELLVFT
                     VQHTEVPLVLDADALNLIAEDAEILSGCRAEKILTPHVGELARLLHMTIAECQRDPAG
                     SAGRAAEKYQACCVRKDSVTVTAEEGREQYYINTSGSSALATAGSGDVLAGITGAFAA
                     KRQCEKNEKKISLAKTAALAAYAHGKAGEAAEEKSSASYVTASEIIRGLQSI"
     gene            complement(10094..10750)
                     /locus_tag="PLN77_03160"
     CDS             complement(10094..10750)
                     /locus_tag="PLN77_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRM43313.1"
                     /translation="MEEKGISRAVIKRLPRYYRYLGELLEDGVERISSGELSERMKVT
                     ASQIRQDLNNFGGFGQQGYGYNVRYLYSEIGKILGLDRVHNMIFVGAGNLGQALANYA
                     AFERSGFKTVGIFDVNPVLKGISVRGIEIRMADELPEFIKEHHVEIAALTLPKAKAVE
                     MAEILVDNGVKAIWNFAHTDLNLKLPKDVIVENVHLSESLMRLSYNLTRYEAEHPKNN
                     "
     gene            10905..12830
                     /locus_tag="PLN77_03165"
     CDS             10905..12830
                     /locus_tag="PLN77_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRM43314.1"
                     /translation="MILACQNISKAFGTTEIIKNASFHIEEREKAALVGINGAGKSTL
                     LKIIIGEMRADEGEVILSKGKTMGYLAQHQDLTGHLSIYQEVLTAKQNLIDMEERLRQ
                     MESDMNTKSGAELDELLSTYTRLSHTFELENGYAYRSEVVGVLKGLGFSESDFEKQVD
                     SLSGGQKTRVALGKLLLTKPDIILLDEPTNHLDMDSISWLETYLLNYSGAVLIVSHDR
                     YFLDRVVTKVVEVDNKKVTTFEGNYTAYSQKKAMLREAAYHAWVNQQQEIHHQEEVIA
                     KLKSFNREKSIKRAESREKMLDKMDVLEKPTEVRSDMRIRLEPRITSGNDVLTVEHLS
                     KAFPGQTLFQDLNFEIKRGERVAIIGNNGTGKTTILKILNEVIRPDVGSFTLGSRVQI
                     GYYDQEHQVLHMEKTIFQEISDAYPYLTNTEIRNVLAAFLFTNDDVFQPIHTLSGGER
                     GRISLAKLMLSNANFLILDEPTNHLDIVSKEILENALKDYTGTVLYVSHDRYFINQTA
                     TRILELTGQTLVNYIGNYDYYLEKKEELTRIYAPAADATAPEETAAPSEASSTKLDWK
                     AQKEEQARLRKKENELKKTEKRIEELETRDSEIDEEMSKPEVATNVAECVKLSKEKAE
                     IAAELEELYEKWEELAE"
     gene            complement(12929..14131)
                     /locus_tag="PLN77_03170"
     CDS             complement(12929..14131)
                     /locus_tag="PLN77_03170"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRM43315.1"
                     /translation="MEKIQMTTPLVEMDGDEMTRILWKMIKDELLLPFIDLKTEYYDL
                     GLEHRNETNDQVTVDSANATKKYGVAVKCATITPNAARMTEYNLKEMWKSPNGTIRAM
                     LDGTVFRTPIVVKGIEPCVRNWKKPITIARHAYGDVYKNAEMRIPGPGKAELVYTAED
                     GTETRELIHNFTGAGVIQGMHNLDNSIESFARSCFEYALSTKQDLWFASKDTISKKYD
                     HRFKDIFQEIFDAEYKEKFAEAGITYFYTLIDDAVARIMKAEGGFIWACKNYDGDVMS
                     DMVSSAFGSLAMMTSVLVSPHGYYEYEAAHGTVQRHYYRHLKGEETSTNSVATIFAWT
                     GALRKRGEMDGNAELSAFADRLEKATIATIESGKMTKDLALITTMENPTVLNSRDFIL
                     AIREELEK"
     gene            complement(14297..15142)
                     /locus_tag="PLN77_03175"
     CDS             complement(14297..15142)
                     /locus_tag="PLN77_03175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRM43316.1"
                     /translation="MKEQENLTGKGITAAVLDTGIFPHMDFDGRIVAFRDLVYGRETP
                     YDDNGHGTHVCGILGGSGRASGGKYQGAAPGCRFVVVKILDRRGNGRKQDILAAIDWV
                     CKERIRLNIRILNISVGTTEQEKTVDDLLVQAVERAWDDGITVVTAAGNLGPAPGSIT
                     APGSSRKVITVGAGDLLEPRRGISGCGPTRDCVCKPDLVAPGKRLISCAPGRSRKEYV
                     VKSGTSMAAPRVSGAVALALERVPDLTNVQAKMLLCESARDLGLPRNRQGHGMLQTDR
                     FLSLL"
     gene            complement(15231..15515)
                     /locus_tag="PLN77_03180"
     CDS             complement(15231..15515)
                     /locus_tag="PLN77_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43317.1"
                     /translation="MRIGVPALRILSVAYLLSIPGLIYQAALQGLSLGKESMYISMTR
                     QAILPLVFAFLLKGFGNLNLIWGAFVLAELCGIPLAAALWKRKGEKQIQF"
BASE COUNT         3588 a         4249 c         3496 g         4318 t
ORIGIN      
        1 gcgtatctat tttctgggcc agctcctcgg atgtgagctg ttttccctta atttccaatg
       61 taatcagata agcgtcatcc cggatatatt tgagaaggcg ctctgcttct ttatttttga
      121 tattcgtctc tacggtttca ctcgcgttgt ccggcgtttt ttcatccgcc acctcgataa
      181 tttccagctt tgcatagcgg ctgaggcgct tcgaatactc ggcgatcgca tcccgcagat
      241 atttctcttt gatttttcct actgttaata tcgtaatttt cattctgtaa tcttaaaacc
      301 ctttcggata tctgtcaaaa acaccaaaag ccatatagat cactttttgt ccgcgtctat
      361 atggctttta gtttagaaaa gcaatgcctt atgcattttt gcttttgata tactcatcaa
      421 ttcctttagc tccggctttt ccggcaccca tagcaaggat aacggttgct gcacctgtta
      481 cagcgtcacc gccggcgtaa acgccctctt tgctggtctt tccggtttct tcctcagcta
      541 cgatacattt tcttctgttg acatccagac cgtaggtggt ggaagaaatc agcgggttcg
      601 gggaagtacc aagagacatg ataacttcgt ctacttcaat ttcgaactca gatcctggaa
      661 tctcaaccgg tcttcttctt ccggaagcgt ccggctcgcc aagttccata cggatgcagc
      721 ggataccggt tacccagtct ttgtcgtcgg tcagaatttc taccgggttg gtcagaaggt
      781 cgaagatgat accttcctct tttgcatgat gaacttcctc tgcacgggcc ggaagctctg
      841 cctcagaacg acggtaaata acgtgtacct ctgcgccgag acgaagtgcg gttcttgcag
      901 cgtccatagc tacgttgccg ccgccgacta cagcaacttt cttgccgtgt gcgatcgggg
      961 tatcgtaatc atcgcggaat gctttcatca ggttgtttct ggtcaggaac tcgtttgcgg
     1021 agaatacgcc gtttgcgttc tcacccggga ttcccatgaa catcggaagg cctgcgccgg
     1081 aaccgatgaa gactgcttca aatccttcgt cttttaacag ctcgtcgatg gtaacggatt
     1141 ttccgatgat aacgttggtc tcaattttaa caccgagaga ttttacgttc tcgatttcag
     1201 cggcaactac tttgtctttc ggaagacgga actccggaat accgtagctt aatacaccgc
     1261 cggctttgtg aagtgcctcg aagatggtta catcgtagcc aagttttgca aggtctcctg
     1321 cgcaggtaag acctgcaggg ccggaaccga taaccgcgat cttgtggccg tttttctctg
     1381 ctgccggaac cggtttgata ccgttttctt ttgcccagtc agcaacgaaa cgctccagtt
     1441 taccgatgga aacagcgtcg cctttgattc cgcggacaca tttgccctcg cactggctct
     1501 cctgcggaca tacacggccg cagactgccg ggagtgcact ggactcaccg ataattttgt
     1561 atgcttcctc gaagtttcct tcttttacct gtgcaatgaa tgccggaatg ttgatattaa
     1621 ccgggcatcc tgtgatacat tttgcatttt tacagcccag gcagcgctgt gcttcttcta
     1681 ctgcttcgtc tttgttgtat ccaagacata cctcgtcaaa atttgtggca cgtaccttcg
     1741 gctcctgctc acgtaccggt actctgttta atccctttgc cattatttgt cacctccgca
     1801 ctggccgcat ccaccgtgat gggttgcgcc ttcctggtat cttaagagag ctcttccctc
     1861 ttctgtcttg tacatctgct gacgtttcat agccaggtcg aagtctacca gatgaccgtc
     1921 aaactccggt ccgtctacgc aggcaaattt tacttcattg cctaccatta gacgacaggc
     1981 accgcacata ccggttccgt ctaccatgat cgggttcatg ctgacaacgg tcgggatgtt
     2041 cagttctttt gtcagaagac atacgaattt catcatgatc atcggtccga tggcaacaca
     2101 gacatcatac tgtttgccct ggttctgtac aaggtcgcgg agtacatcgg tacccatacc
     2161 ttttcttacg taagaaccat cgtctgtggt tatgtacaga ttgctgacag cagacatttc
     2221 tttctcgaga ataacgagat cttttgtctt tgctccgaca atggcgtcag cagtgattcc
     2281 atgctcatgc agccatttta actgcgggta aaccggtgca gttccaacac cgccggctac
     2341 gaatacgatc cgttttttct tcagttcttc gatatcttcg ctgcagaact cagacggctg
     2401 tcccagaggt cctacgaaat ccatgaaagc atctcccgct ttgagatctt tcattttttt
     2461 ggtagattct ccgatcggca tgaatacgat ggtgatagtt cctgcttctc tatcgtaatc
     2521 acaaatggtc agaggaattc tttctccttc ttcatctgcc ttgacaataa caaactgtcc
     2581 agggaggcag tgctgtgcta ctcgcggtgc ttctactacc atacggtaga tgttattcgc
     2641 gagcatttcc gcttcttgaa tcttgtacat aatatcctcc taatcttgtt agaaatattt
     2701 taacaaaagc acgacttgga ttttcgcgca ttttatgtta atactttatc acgtttccgg
     2761 ttatttgtaa acaacggaca acatcttttc ccatcatccg tgttcctcca gataattttc
     2821 gaactgttcc atggacatca gaacttttct cggcttggtt ccttcctccg gtcctacgac
     2881 accggcatcc gcgagctggt ccatgattct ggccgcgcgg ttgaaaccga ttttcagcac
     2941 tctctggagc atgccgatcg atgccttatc tttttcgatg ataaattttc cggcttccac
     3001 gaagtaatta tcgcggttat ctccgccgcc tgcggcagaa ccgccatcca ggcttacggt
     3061 gtgaatccgt ttttcgatat ccggatcata agcgttctcg ccgtagtgat cttttaagta
     3121 ggcaacgaca tcgctgacct ctttatccga aacaaaggat ccctgcagac gggccggttt
     3181 ctggtaatcc tgcggcttgt agagcatatc gccttttcca agcagttttt ccgcacctcc
     3241 catgtcgagg atcgtacggg aatctacgct ggaagaaacc gagaatgcaa tacgggacgg
     3301 catatttgcc ttgataagac cagtgatgac attgacggaa ggtctctgtg ttgcaataat
     3361 cagatggatg ccggccgcac gggcaagctg tgccagacgg cagatcgcat cctccacttc
     3421 tcccggcgca accatcatca ggtcggcaag ctcgtccacg ataatgacga tctgcggcag
     3481 tttttccggt tttttgccgt tttcatccgg cggcagctcg gcaactttcg cattgtagcc
     3541 tttcatatcg cgcacgttga attccgcaaa tttgttataa cggtctgtca tctccgcaac
     3601 cgcccagttc agagctccgg aagccttttt cgggtctgtg acaacaggga taaaaaggtg
     3661 cggaatgccg ttataaacag aaagctcaac gaccttcggg tcgatcatga tcagtttgac
     3721 gtcttccggt tttgccttat aaataatact cataatcagc gtgttgatgc agaccgattt
     3781 accggaaccg gtcgcaccgg cgatcagaag atgaggcatt ttcgcgatat ctgcgatgac
     3841 cggctgaccg gcgatgtctt ttccgacagc aaaggccaga ttcgaagaaa agttcttaaa
     3901 ctccggtgtt tcaagaagat cacgcagcat cacggccgtg ttcgtcttat ttggaacctc
     3961 gattccgact gcggattttc ccgggatcgg tgcctcaatg cggacatctg ctgctgcaag
     4021 actcagcttg atatcgtcgg tcaatccgac aatccggctt actttcacgc cctgttccgg
     4081 catcagctca taccgtgtga cggtcggtcc gcagcttacg ttattgactg taactttgac
     4141 accgaagctg tccagcgtct cctccagttt ccgcgctact tcccgcagat gcgcatcgga
     4201 atcgcccatg gcaccgcgcg tccctttttt cagaagattc atcggcggtg ttttgtactc
     4261 cggccgcttt tcctctgctt ttttcccgat ttcctgacga atgccgtcta catccgcctc
     4321 gatctcttca taggaagacc gcggatttct cgtatgatcc gcagacgttt tctctttttt
     4381 cgcctcttct gcagcttgcc gtgtcatctc cggttccgtc ttttccggct cttcagccgg
     4441 cacagaacca cggtgaatcg aaagctccgg catctccatc tgatgggtat cgtcgttttc
     4501 gtttaaatca gccttcgatt ccacatgaga aagaaggcgg gatttctttt tcttcccggc
     4561 ttttttcggt tcctccactg gctcaagaat cagctctttt ccttcctctt cctccatcgc
     4621 agcttggcgc gccagacgct cctggcggcg ctcctccgcc atccggcggt ggtcattcgt
     4681 cttttcatgg atcggcgcga gaatcggctt ctgcgtcatc acgatcacgg aaatcacgat
     4741 ggcgatcaca ataatcaccc acgcgccgat ctggccgaat cccatgccga gaattttcac
     4801 gacagaccct cctaagattc ctccgccgag atgatcgtag gcgctgtcaa aatagtaatc
     4861 cagaaatgtc gtatctctcg tatagccgga cgccagaagc tgcataaaag cgcagaaaaa
     4921 gacgaaacag ctgatcgctc ccccggtttt ccgcacaatt aaaaagttct gtctgtttgc
     4981 tatgtaaaat gctgttccaa agaagatcag aaccggtgct gcccactctg tcacgccgaa
     5041 aaggccgaaa aagaagtcgc agacagcctg tccgatcacg ccgcccatac cgaaattact
     5101 gagcatcagc agaatcgaaa cagcaagcac ggaaagcaga ataatttctg cgcgcactcc
     5161 ctcgtccatc ggctcacgga ctgtgttttt ccttgtgccg gaggtacgtt ttgcggtact
     5221 gctctttttc accgttgttt ttctggccgt tgtttgggtc tgtttctttt ttgctgtccc
     5281 ggaagcagaa gtttttttct ttgtcttccc gcttcccttt gttgtatttg ccatgcagaa
     5341 cttctcccct ttctcgtatt acagcagaaa atatcctgca atggagaatg tcccgacaag
     5401 aagacagtac aaagcaaaat atttcagttt ccgttttttc accacacgca tcagaatgcg
     5461 gatcgtaaag taaccggcaa gtgccgccat gaaaacgccg accagatata ccggatgaaa
     5521 aacaatttta ttttttccca tggtcgcaat ttcaacgatc gttgccccga taatcgccgg
     5581 aacactcaca atcagagagt atttcacagc gtacgaacgg gtcatgccgc cgatcacaca
     5641 cgcagaaata gtcgaaccaa gacgggaaag acccggaatc gtggaaaagc cttggaagaa
     5701 tccggcaatc agtgcatctt tcggcttcaa atttttctgc gttttttctc cctccggaag
     5761 catatctgcc accacaagca gaatcgccgt cagatacaga ccaacgccag gtgccagcag
     5821 attcgaggag gtcagtgtga caaggctctg aatcatgccg ccgattacag cagtcgggat
     5881 ggtggtcaga atcagcagga aagcaagcat acggtagttg ctgccgagta ccttccggta
     5941 ctgctttctc tcctgatgct gactggaaaa gtgttccttt aaattatgaa acagatcgat
     6001 tatgattccg cacaacgcga ggaagagacg tttccattcc ttcgaaaatg tgagcagaat
     6061 tgcaatcatg gagccaaagt gcaaaagcac attgaaaaac accgtctgcg tatcttcgat
     6121 cttcataaag tgacggaaga taatcagatg tgcggagctg ctgatcggca ggaattccgt
     6181 gattccctgg atcaggccca gaaataaagc ttctattact gtcatttttt tatcctgttc
     6241 ccttattttt ctcttttgtg aaagtccgga caaaacttcc gttcgaattt tcacagcgaa
     6301 ctcattctaa cacaaaatcc cctgaaacac aagccgcgcc gtgaagtcaa ttttctgtcc
     6361 ctgctgcctt tttcatcagc tcattcagct tttcgagcgc gcgggcaatg ccgccgacct
     6421 cgtcgatcag gccttcttcc actgcctgcg ggccgtccag cattgttccg acatccttga
     6481 ccagcatttt cgtatcgagc atcagctcct cgagccgttc cttggtgatt ttagaatggg
     6541 ccgatacaaa acctgtgatc cgatcctgcg tcttttccat attacggtac gtctgtgcaa
     6601 caccgatgaa catgccgttt gtacgcaccg ggtgaatgac catggtggca ctcggcacga
     6661 tcagcgaata gtctgctgca acggcgagcg ggacaccgat cgaatgaccg ccccctaaaa
     6721 cgagcgatac cgcaggcttt cgcatggacg caatcatctc tgcaattgca agaccggcct
     6781 ccacatctcc gcctacggta ttcaggagaa tcagaagacc atcaatactt ttgtcctcct
     6841 cggcacgggc aagagcaggg agaatgtgct catagcgcgt cgctttcgtg cgctcgccga
     6901 gtgattcatg cccctccacc tcgccgatga tggacagcag ataaatgttg gtcttcagtt
     6961 ctacaatatt ttctttttct gtctggtttt tctgtgattc tgccatacgg atttcctctt
     7021 ttctgctatt ccaaaatagc ttcatcagta tgcgcagatt cttatgattc tatgtaattt
     7081 ccagtgaaat tactcgtcca ggttggtgta tacgtactgt acgtcgtcgt cgtcatccag
     7141 cagatccagg gtacggttca ggtcttttaa tgcctgcgga tcggtcagct ctacgtaggt
     7201 ctgtgggatc atggtaacgt ctgcctctgc catggcgata ccgtctttct ccagagcctc
     7261 acgtactgcg gagaagtcat ccggatcggt gatgatttcg tagctgtcct cttcgtctgc
     7321 gaagtcttct gcgcctgcat ccagagcaag catcatcaga tcgtcagcat ccatttcgca
     7381 ctcttctttg tcgatgatga tctggccttt tttgtcgaac atgtaggata cgcatcccgg
     7441 tgttccaacg cttccgtttc ctttggtgaa agcgcttctt acgtttgctg cggtacggtt
     7501 cttgttgtcg gtcagagtct ctacgatgat cgcgatacca ttcggaccat aaccttcgta
     7561 ggtgatacgc tcgtagttgt ctgcgtttgc atctccggca gcttttttaa taccgcggtc
     7621 gatggtatcg ttcggcatgt tgtttgcttt tgcttttgcg ataacgtcac gcagcttgct
     7681 gttgtttgcc ggatccggtc cgccctcttt gacagcgatt acgatctcac ggccgatgat
     7741 ggtaaagatt ttaccttttt ttgcgtcgtt tttttctttc ttatgcttaa tattggcaaa
     7801 ttttgaatgt cctgacatac ctcttttgtc tcctttattt aaagtattgc ttttctgtaa
     7861 aaaggtttcc ccgaaaaacg gcagaagccc atataatata gaatatagca cgatctttct
     7921 cattcgtcaa ccgtgaaccg cgccaaaaat accccttatg taggcagatc caggctgatt
     7981 ttcagtgctt cgttcacaag ggccagctgt tccggcgtca gatgacagat tttatctttc
     8041 agccttctct tatcgatcgt ccgaagctgt tccagtaaaa taacagaatc ccgtacaatc
     8101 ccataggcgg acgcttcgat ctcaatgtgc gtcggcagct tcgccttatt catcctcgat
     8161 gtaatcgccg cacagatcac cgtcggactg tgccggttgc cgatgtcatt ctgaatgatc
     8221 agcaccggac ggacaccgcc ctgctcactt cccacaacgg gccgcaggtc ggcataatat
     8281 acatcgccac gcttgattac cacttgtgct cactctccga ttctttccgt tttgctttcc
     8341 catacgataa cgatccggcg ttttacgcct tttaaaatcg tttccatgta tttaatattg
     8401 cctgtttttc cggaggtatt cacaaatttc atccggcact tcaaaaaagt ccgcccggag
     8461 tccttttggg aacccgtgat tctgatctgc tttgctcaga ttgactgcaa tcctctgata
     8521 atctcggatg ctgttacata ggaagcactg cttttttctt ccgcagcctc tcccgccttt
     8581 ccatgcgcat atgccgccag tgccgctgtt ttggcaaggc tgattttttt ctcatttttt
     8641 tcacactgac gttttgctgc aaaggcgcct gtgattcccg cgagcacatc gccgcttccc
     8701 gccgtcgcaa gcgcactgct tccgcttgtg tttatgtaat actgctcacg cccttcctct
     8761 gctgtcacgg ttacggaatc ctttcgtaca cagcaggcct ggtatttttc ggctgccctt
     8821 ccggcgcttc ctgccgggtc acgctggcat tctgcaatgg tcatgtgcag cagccgtgca
     8881 agctctccta catgaggcgt taaaattttt tctgcccggc agcccgaaag aatctctgca
     8941 tcttctgcaa tcaggttcag cgcatctgca tccagaacaa gaggaacttc tgtgtgctgc
     9001 accgtaaaaa cgagcagttc ttttgccgtc cggcttttgg aaagccccgg tcccgcaaca
     9061 acagcgtccg cccaggcgag atcctgcagc agggcttctt ccggaagcgg ctcatcctcc
     9121 ttccagaaat ccgccattgc ttccggcagg gcacagaaaa gcggcgtacg gtttctttcc
     9181 tctgtcacaa cctttaccat gccggcaccg gatgcaagga ctgccctggc acacaaaaca
     9241 gcggctccgc acatggcacc ggaaccggca agtaccagca cctttccgaa ggttccctta
     9301 tttcccatcg gcgtgcgggt aagaaaccgg tccagatctt ctttttccac tgaaccgtac
     9361 cagggcgtct cctcttctcc tgcatcaaag ccgattccga tatcacggac caggacctct
     9421 ccggcatatc gttttccggg atcaaaaaga agccctggct ttgcacaggc aaatgtcacc
     9481 gtggctctgg ctttcaccgc cgcatcaagg acctctccgg tatccgtgtg gatccccgaa
     9541 gggatgtcca cggcaagaac aggaacaccg cttgcgcaga ttgcttccac tgccctccgg
     9601 tactcccctg ccagcggacg ggatacgcca attccaaaga tggcatctat gataacagta
     9661 tattcccgga aatccggatt cttaacctca cggaccccat aattttccgc aattttcttc
     9721 tgtgctctca tgccttcact gtatttttcc ggatttccaa gacagaacag ttccgcatcc
     9781 acccctttcg tctttaaaat ccgtgcaatt gccgcaccgt cccctccatt atttccggga
     9841 ccgcacacag caaggacttt tcctgtatcc catgttccat tttgaagctc ctccacgcag
     9901 gacagcgctg cgcgctccat cagtaccagg gacggtaccc ccatggtttc gatcgtattg
     9961 cggtctgcct gcttcatctg tgctgctgta agaatccgtt tcatcgcttt tcctcctttt
    10021 cctgccttat ccggaaagtg aacagaaaaa gtctccggtt tcccgaagac tctcctgctt
    10081 ttgttttggt ttcttaattg ttctttggat gctccgcctc atatctggtc agattgtagg
    10141 agagacgcat cagactttcg gaaagatgga cgttctcgac gatcacatct ttcggaagtt
    10201 ttaagtttag atcggtatgt gcaaaattcc agattgcttt tacaccgttg tccacgagaa
    10261 tctctgccat ctctaccgct tttgcttttg gcagtgtcaa agccgcaatc tctacatggt
    10321 gttccttgat aaattccgga agctcgtccg ccatgcggat ttcaattccg cgcacggaga
    10381 ttccttttaa taccgggttg acgtcaaaaa taccgaccgt tttgaatccg cttctttcaa
    10441 aggccgcata attagcaagc gcctgtccca gatttccggc tccgacaaaa atcatattgt
    10501 ggactctgtc cagacctaag atttttccaa tctcgctgta cagataccgc acgttataac
    10561 catacccctg ctggccgaag ccgccgaaat tatttaaatc ctggcgaatc tgggaagctg
    10621 tcactttcat ccgctcgctc agctcaccgg aagagatccg ttctactccg tcctccagca
    10681 attcgcccag gtaacgataa tatctaggaa gtcttttgat aactgcccgc gaaattcctt
    10741 tttcttccaa gataaattcc tccttattta cacctgcata tattcatatt atatatggtt
    10801 gatagaaaat tgtcaaagat ttcttgaaat tttgtgtatt actctctata ataaggatgg
    10861 tgtattatgt caaaatacag aaatatgtta tctggaggaa ccacatgatt cttgcatgtc
    10921 agaatatctc gaaagcattc gggacaacgg aaattatcaa aaatgcatct tttcatatag
    10981 aagaacgcga aaaagccgca ctggtcggca tcaacggtgc cggaaaatcg acgctgttaa
    11041 aaatcatcat cggcgaaatg agggccgatg agggagaggt catcctctcc aaaggaaaga
    11101 cgatgggcta tctcgcccag catcaggatc tgaccggaca cctttccatt taccaggaag
    11161 tgctgaccgc gaaacagaat ctgatcgata tggaggaacg cctccgtcag atggaaagtg
    11221 atatgaatac aaaatccggt gcagagctgg atgaacttct ttccacctac acccgcctct
    11281 cccatacctt tgaactggaa aacggatacg cgtaccgcag tgaagtcgtc ggtgttttaa
    11341 aaggtcttgg cttttccgag agcgattttg aaaaacaggt ggactccctc tccggcggtc
    11401 agaagacccg cgtggcactt ggtaaactgc tgctcaccaa accggacatc attctgctcg
    11461 acgagccgac caaccacctc gatatggact cgatctcctg gctcgaaacg tatcttttaa
    11521 attacagcgg agccgtcctg atcgtctccc atgaccgtta ctttctcgac cgcgttgtca
    11581 caaaagtcgt tgaagtggac aataaaaaag taacaacttt tgagggaaac tataccgcct
    11641 acagtcagaa aaaagcaatg ctccgtgaag ccgcttacca tgcctgggtc aaccagcagc
    11701 aggaaatcca ccatcaggaa gaagtcattg caaagctgaa atctttcaac cgcgagaaat
    11761 ccatcaaacg cgccgagagc cgtgaaaaaa tgctggacaa aatggatgtg ctggaaaagc
    11821 cgaccgaagt acgctccgat atgcggatcc gtctggaacc gcgcatcaca agcggaaacg
    11881 atgtgctgac cgtcgagcac ctctccaaag ccttcccggg acagacgctt ttccaggatc
    11941 tgaatttcga aatcaagcgc ggcgagcgcg tggcaatcat cggaaacaac ggaacgggaa
    12001 aaacgacgat cttaaagatc ttaaacgaag tcatccgccc ggatgtcggc tcttttacac
    12061 ttggaagccg tgtacagatc ggttactacg atcaggagca tcaggtgctc cacatggaaa
    12121 agaccatttt ccaggagatt tccgacgcgt atccatatct gacaaacacc gaaatccgaa
    12181 atgtgctggc cgccttcctg tttacaaacg acgatgtctt ccaaccgatt cataccttga
    12241 gcggcggtga gcgcggacga atttcccttg ccaagctgat gctctcgaac gccaacttcc
    12301 tgatcctcga cgagccgacc aaccacctcg atatcgtatc aaaggagatt ctggaaaacg
    12361 cgctgaagga ttacactgga acggtactgt acgtttccca cgaccgttac tttatcaatc
    12421 agactgcaac ccggattctg gagctgacgg gacagacact cgtcaactat atcggaaatt
    12481 acgattacta tctggagaaa aaagaagagc tgacccgcat ctatgctcct gctgccgatg
    12541 ccaccgctcc ggaagaaaca gcggctcctt ccgaagcctc ctccacaaag ctggactgga
    12601 aagcgcagaa agaagaacag gcacgtctcc gcaagaaaga aaacgagctg aaaaagacgg
    12661 agaaacgaat tgaagagctt gagacacgcg attcggaaat tgatgaggaa atgtcgaaac
    12721 cggaagtcgc aacgaatgtg gcagagtgtg tgaaattaag caaagagaaa gcagagattg
    12781 ccgcggaact cgaagagctg tatgaaaaat gggaagagct tgccgaatag gcctggtttt
    12841 ccccggaaaa ttcaaaacag aaaaagccgg tccggcgcat ccctttttgc gggaatgccg
    12901 gatcggcttt cttttttgct tcgtttaatt atttctccag ctcctcgcgg atggccagga
    12961 tgaaatcacg gctgtttaat acggtcggat tttccatggt ggtgatcagc gcaaggtctt
    13021 ttgtcatttt tccgctctcg atcgttgcga tcgttgcttt ttccagacgg tctgcaaatg
    13081 cggaaagttc tgcgtttcca tccatttccc cgcgttttct gagtgcaccg gtccatgcaa
    13141 aaatggtggc cacggagttg gtggatgtct cctcaccttt taagtgacgg taatagtggc
    13201 gctgtacggt tccgtgcgca gcctcgtact cgtaatatcc gtgcggggat acgagaacag
    13261 aagtcatcat cgcaagggaa ccgaaggcgg aggataccat gtcgctcatg acatcgccgt
    13321 cgtagttctt gcaggcccag ataaagccgc cctctgcttt catgatgcgg gcaacggcgt
    13381 cgtcgatcag agtgtagaag taggtaatgc ctgcttctgc aaatttctct ttgtactctg
    13441 catcgaagat ctcctggaag atatccttga agcggtgatc atacttcttg gaaatcgtat
    13501 ccttgcttgc aaaccacaga tcctgtttcg tggacagtgc gtactcgaaa cagcttctcg
    13561 caaagctttc tatggagtta tccagattgt gcatgccctg aatgacaccg gcgccggtga
    13621 agttgtggat cagctctctt gtctcggtgc catcctcagc cgtataaacc agctctgctt
    13681 ttcccggtcc cggaatgcgc atttctgcat ttttatagac atctccgtat gcatgacggg
    13741 caatcgtgat cggttttttc cagttgcgca cgcacggctc gatgcctttt acgacaatcg
    13801 gggtacggaa aacggttccg tcgagcatgg cacggatcgt tccgttcggg ctcttccaca
    13861 tttctttcag attgtactcg gtcatacgcg ctgcattcgg cgtaatggtg gcacatttga
    13921 ctgctacacc gtatttcttg gtagcgtttg cggaatctac ggttacctga tcgttcgtct
    13981 cgtttctgtg ttccagtccc agatcgtaat attccgtttt caggtcaata aacgggagaa
    14041 gcagctcatc tttgatcatt ttccagagaa ttctggtcat ctcatctcca tccatctcta
    14101 ccagaggagt ggtcatctga attttttcca tagtaggcct cctttttcta ttcccgttta
    14161 ttgtataaag tatcctatag cttgtcaagt caaaattcgc ctgatttttt ggcattttcc
    14221 cataaattca cacaattttt gcaggatgag aggtgttttc cttcattttt ctttttttat
    14281 gctgccgcac agtgtctcac agaagagaca gaaaacggtc tgtttgaagc atcccatgcc
    14341 cctgacggtt gcgcggaagg ccaaggtctc tcgccgattc gcaaagcagc attttcgcct
    14401 ggacatttgt aagatccgga acccgttcaa gtgccaaagc cacggctccg gacacccgcg
    14461 gtgccgccat cgaggtgccg ctcttcacca catattcctt cctgcttctt ccaggcgcgc
    14521 aggaaatgag acgttttcca ggcgcgacaa gatccggttt gcagacgcag tcccgtgtcg
    14581 ggccgcatcc ggagattcct cttcttggct ccagcagatc gcccgctccc acggtgatca
    14641 cttttctgct gctccccggc gccgtaatac tgcccggagc gggccccaga tttccggccg
    14701 ctgtcacaac agtgatcccg tcatcccagg cgcgctccac cgcctgtact agcagatcat
    14761 ccaccgtttt ttcctgctct gttgttccca ctgaaatatt taaaatgcgg atattaagtc
    14821 ttattcgctc tttgcacacc cagtcaatcg ccgcgaggat atcctgcttt cttccatttc
    14881 cccttcggtc cagaatcttc actacgacaa accggcatcc cggtgccgca ccctggtatt
    14941 ttccgccgga cgctcttcca cttccgccaa ggataccaca gacatgcgtt ccgtgtccat
    15001 tgtcatcgta aggcgtctcc cgcccgtaca caagatcccg gaaggctaca atccggccgt
    15061 caaaatccat atggggaaaa atccctgtat ccagcacggc ggctgtaatt ccttttcctg
    15121 tcagattttc ctgctctttc atccataagc ccctgtgtat tcttatctta tggtatgcaa
    15181 aaaaggctgc ctttcgcaaa gaacagcctt tttgtttttc ttttctatac ttaaaactga
    15241 atctgctttt cccctttccg tttccagaga gctgctgcaa gcgggatgcc gcagagctca
    15301 gccaggacaa aggcgcccca tatcagattc aggtttccaa atcctttgag aagaaatgca
    15361 aacaccagcg gcagaatcgc ctggcgtgtc atggatatgt acatactttc ttttccaagg
    15421 gaaagtccct gcaacgccgc ctgatagatc agacctggaa tcgaaagcag ataggcgacc
    15481 gacagaatgc ggagcgccgg gactccaatg cgcatcagat tgtccgaggc atcatattgt
    15541 atgtatggca ataaaaaaca ggaagatatc tgccaaaacc gtttcggtaa agatcttcct
    15601 gtcctttttt cagaaacttt tcaaaaacat tacttctgct gctccattcg c
//
LOCUS       DAOMUF010000029        15607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_772_length_15607_cov_3.658436, whole genome shotgun sequence.
ACCESSION   DAOMUF010000029 DAOMUF010000000
VERSION     DAOMUF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 15607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15607
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_772_length_15607_cov_3.658436"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLN77_03185"
     CDS             complement(<1..620)
                     /locus_tag="PLN77_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM43318.1"
                     /translation="MLQAALPNGFRLRMLPRSVPAVFRQTGEIFYIGGADVLPAPLEG
                     QAEADAIAGLSDPENETARSELIEHNLRLVVYIAQKFDNTGVGVEDLISIGTIGLIKA
                     INTFNPEKKIKLATYASRCIENEILMYLRRNNKTRMEVSIDEPLNVDWDGNELLLSDI
                     LGTEEDVIYQGLEQEAEHRVLGSAISKLSEREQVIVKLRFGINMPEG"
     gene            complement(624..1487)
                     /locus_tag="PLN77_03190"
     CDS             complement(624..1487)
                     /locus_tag="PLN77_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HRM43319.1"
                     /translation="MHYEIFLDLLFAENLLLNYTILRLSSFLLKRSATRRRSFAAAAL
                     GAGYAVLYAVLARYLPGALLALVGIGITTGMVKSGCRITTVRDLAVGMAGYILSGFLI
                     GSAYRLFRELMPGRGILLFLLSGSAAYAGTLLLLKIRKEMLLEHARQVTVQLVQNGKW
                     KKVKGLYDTGNTLRDATTKKPVSVVPYTVILELFPDEMRTGIAALARHETVPEPERLL
                     ALQPRYIPFRGLEGGGFLPVIRISEMILVTEHTARHISGPLIALGGENSSSPRGYEII
                     LHPDLMEGQEE"
     gene            complement(1677..2858)
                     /gene="ftsZ"
                     /locus_tag="PLN77_03195"
     CDS             complement(1677..2858)
                     /gene="ftsZ"
                     /locus_tag="PLN77_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRM43320.1"
                     /translation="MTNEAESSAKIIVIGVGGAGNNAVNRMVEESIGGVEFVGINTDK
                     QALTLCKAPTILQIGEKLTKGLGAGAKPEVGEKAAEESIEELKQIMEGADMVFVTCGM
                     GGGTGTGAAPVVAGVAKEMGILTVGVVTKPFKFEARTRMSNALMGIEKLEENVDTLIV
                     IPNDKLLEIVDRRTTMPEALKKADEVLQQAVQGITDLINLPALINLDFADVQTVMTDK
                     GIAHIGIGEAKGDDKALEAVQQAVSSPLLETTIEGASHVIINISGDISLMDANDAASY
                     VQNLAGEDANIIFGAMYDDSVTDTAKITVIATGLNDATAKQASVSRKEPADARKSAFS
                     SSSFSAQAAAPVHTAPKASAPVQPQPQVQVQPQVQAQQTMTSNVPKKDIQIPDFLRNR
                     R"
     gene            complement(3044..3991)
                     /locus_tag="PLN77_03200"
     CDS             complement(3044..3991)
                     /locus_tag="PLN77_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43321.1"
                     /translation="MRQKKNRKKKKNRKIRILTGVVLGVGLAGALFFNVFKIRNIQVA
                     GNTRYTKDEIKEMVADDKWMKNTVLLTTFHSKVDMSDIPFMNSVEFTFVSYDTICIQV
                     NEKKVVGYVEYEGQKVYFDKNGIVLECVDEEQETPAEGEPSDGTASDGTSADGSLTSE
                     PLASESVSSDGTASESSTSDGALSADTIKSARVTTTDFQPTLSDVPLVTGLTFDYVTV
                     NEQLPVEKPAVFRTMLALTRMTEKYNITPDSVEFNENSEIILHYYDTVRILLGTDTAL
                     EEKMTRVAAILPSLSGLSGELHMEDYKDGDSSFVFSKDV"
     gene            complement(3988..5253)
                     /gene="murA"
                     /locus_tag="PLN77_03205"
     CDS             complement(3988..5253)
                     /gene="murA"
                     /locus_tag="PLN77_03205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM43322.1"
                     /translation="MGVYQILGGRRLCGTVTVQGSKNAVLPMIAAAVLTKEEVVLEGC
                     PKISDVEDMAEIVRSLGGTVWWERNALHLNCEKIEKSRVEGALSKRLRASLLFLGSLL
                     ARTGEAYLAGAGGCRIGKRPTDLHQRAMELLGAEVFEEDGTIRAKADHPKGAVLCFPK
                     KSVGATENAVLFAVGAEGATRLEHCAREPEVVHLCRFLKAMGAEITGEGTEQITVYGR
                     QGKRLLSGCRYRVPGDRIAAGTYLLMGAATRGHLTLSGAPLDEMGAVLSLYQKIGGQY
                     TRKSGTLVADSKNVQHAVPYVETEEYPGFPTDLQSLYLAAAMTLEGKSCICENVFEDR
                     LRTAEELQKMGGTLQICGKWLTAEKSRLHGAEVVSQDLRGGAALVSAALAAEGFSTIR
                     QTELIERGYERFPETLRELGAKIIVEKEI"
     gene            complement(5338..6420)
                     /locus_tag="PLN77_03210"
     CDS             complement(5338..6420)
                     /locus_tag="PLN77_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRM43323.1"
                     /translation="MRKEKEKKIDGTLLVTVWILLGLGLVILYSASAYNGRVRFHDPA
                     YYFKKQLFASALGVIGMYAVAKIDYRRLFELAPWFYAASLALSTAVLFFGSEYNGSKR
                     WLKLGPLSFQPSEFAKMAVILFLPWVMEHTKGRSDSLWFMTKAVLCTLPIAALVGSNN
                     LSTAIILLGICAFLIFISHPGYLPFLGIGGAGIGFIAVFLMMESYRLERLAIWRNPEA
                     YEKGFQTIQGLYAIGSGRIFGCGLGKGLQKLGFVPEAQNDMIFSIICEEGGLFGACLV
                     IAVFAVLIWRLMVISIHARDLGGALIAGGIMGHIALQVILNIAVVTNTIPNTGITLPF
                     ISYGGTSVLFLLGEMGLALSVSRDTD"
     gene            complement(6523..7890)
                     /gene="murD"
                     /locus_tag="PLN77_03215"
     CDS             complement(6523..7890)
                     /gene="murD"
                     /locus_tag="PLN77_03215"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRM43324.1"
                     /translation="MIFAGKKVLVFGTGKSGIAAEDLLHKKGASVVLYDGNDKLEKEE
                     ILKKLESRDSVEVVLGELPEEMLDSLDLVVLSPGVPTDLPVVLKMKEKEIPVIGEVEL
                     AYQVSRGKVLAITGTNGKTTTTSLLGEIMKAYQPEVKVVGNIGTPYTSAALDTTDDTV
                     TVAEISSFQLETIKEFHPAASAITNITEDHLNRHHTMEEYIRVKECITENQTMDDLCV
                     LNYEDEILRPFGEKLMAEKKVQVMYFSSLRALKDGIYYQDGEIRIAKNGETELLTRTD
                     DLKLLGLHNFENVMVASAMALHAGVPMETVRKVIQEFAGVEHRIEYVCEKDGVAYYND
                     SKGTNPDAAIKGIQAMNRPTLLIGGGYDKQSTYEEWIQAFDGKVRFLVLIGDTKEKIA
                     KAARSVGFNDIIMADNLKEAVQICHDKANAGDAVLLSPACASWDQFKSYEQRGELFKE
                     YVRAL"
     gene            complement(7910..8866)
                     /gene="mraY"
                     /locus_tag="PLN77_03220"
     CDS             complement(7910..8866)
                     /gene="mraY"
                     /locus_tag="PLN77_03220"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRM43325.1"
                     /translation="MKREMILPVLLSFGISLALGPVLIPFLRKVKAGQKEREEGVPSH
                     QKKAGTPTMGGVMFLAAFTAVSLLFRKEGAEVVPVLFLTLGFGLIGFLDDYLKVVLHR
                     SDGLYPKQKMALQAVITAVFLWYLARFSGISLRMRIPFLPGKELDAGIFTIPFLAFVI
                     LGTVNGVNFTDGLDGLAASVTILNAVFFSVASIGLGNGVSPSPLAVIGSLLGFLVFNV
                     FPARVFMGDTGSLALGGFVSGMACFLRLPLFLPLVGFIYMMEILSVILQVSFFKLTKG
                     KRIFKMTPIHHHFELMGWSETQIVTVFATITAILCLIALMGL"
     gene            complement(9107..10840)
                     /locus_tag="PLN77_03225"
     CDS             complement(9107..10840)
                     /locus_tag="PLN77_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM43326.1"
                     /translation="MRKNKTYHRKKTVLLWVLCMGMMVGLAGRLVYLCVFRSVYYSEK
                     ADDLHERERGIKAARGRILDANGEVLAANRTVCTISVIHSQIEEPEAVIAMLVKELGI
                     SEETARKRVEKRSSIERVKTNVDKETGDTIRSYGYAGVKVDEDFKRYYPKGKLASRVI
                     GFTGGDNQGIIGLEVEYEEILKGINGKILTTTDARGIEQNGIGESRQEPVAGRDLKVS
                     LDVNIQAYCQQAAEKAMAEKQADSVSILLMNPQNGEIYACVNVPEFDLNDPFTLQQDT
                     TGLSEKEIQDLLNQMWRNACINDTYEPGSTFKIITMSAGLSEGVVSPNDSFFCPGYKI
                     VEDRRIHCHKRTGHGAENFVQGAQNSCNPVFIEVGLRLGVEKFCDYFRNFGLMEKTGI
                     DLPGEASTIMHKEENIGEVELATMAFGQSFQITPIRLAATVSALVNGGKMVTPHVATD
                     VLDEEGNVVKHLNFPEKEGVLSEETSRTVREILESVVSEGSGKNAYLEGYSIGGKTAT
                     SQTLPRSANRYISSFLGFTPAEHPTVLGLCIIRNPQGVYYGGTICAPVIRDIFSNVLP
                     YLGIGHNSQET"
     gene            complement(10923..12977)
                     /locus_tag="PLN77_03230"
     CDS             complement(10923..12977)
                     /locus_tag="PLN77_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM43327.1"
                     /translation="MSRKLVALFLGVMLVFVALAIDITYVNATKGEKYERQVLSQTQQ
                     SYDSRTIPFQRGSITDRNGTILATSEKVYNVILDCKLANTTVKDEEKNDTHPYVDPTV
                     AALVEYFGLDETDIRDRLTGETTKESQYQVLAREVSMDAKKAFETYVDEYADKKNKEL
                     DENEQAEKAYRANIRGVWFEESYHRTYPLNSLACDLIGFTYSGDTADWGIEGYYSSIL
                     NGVNGRQFGYYNEDADMEQTIIEAQPGKNVVTTIDVNIQKIIRTAIENYNERIHVQNG
                     ADESDTETNRQTKAAKNIGVVVMDPNNGEILGMDSSDWYDLNNPRDLTPFYSQEEIDA
                     MNDNETMEALSAIWKNYCISDAYEPGSTAKPMNVAAAYSLDVIDDDTLFDCEGFETIA
                     GQMIRCGAYPGTHGVQTPADVLKNSCNAGMMQIGQKMGAAEFLRYQDIFGFGSLTGID
                     LPGEAYGLVHTEDTMGPTELATSTFGQGYTVTMVQQAAAFSSLINGGNYYQPHVMKTI
                     TDSTGAVVESNEPNLVRQTVSAEISDKMRSFLQGVVTDGSGQSAKVAGYTMGGKTGTA
                     QKYPRGNGKYLVSFIGFAPVENPKAVVYAIVDEPDVENQANSVLAQEIVKEIYTELLP
                     YLNVFPDDTDGVTGEGSETGTDDPNVAAPVQEDDTENSAENSNIENGGLTNAELDLIN
                     EE"
     gene            complement(13052..13561)
                     /locus_tag="PLN77_03235"
     CDS             complement(13052..13561)
                     /locus_tag="PLN77_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43328.1"
                     /translation="MARRADGRVNSTDRTKRYTPDTFEDGSAVRRLAAQPQEAPKEAR
                     PLKSGVSSQTRRNRARATQISAGYAVFLAIVCTLCIAACVAYVKLQTVVTTQAEDITS
                     KEKELSDLKADNDAYYNTTVASEDLEAVKEAAQNRLGMKYADESQVIYFDDSENSYVR
                     QYQDVPDAK"
     gene            complement(13591..14529)
                     /gene="rsmH"
                     /locus_tag="PLN77_03240"
     CDS             complement(13591..14529)
                     /gene="rsmH"
                     /locus_tag="PLN77_03240"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRM43329.1"
                     /translation="MEFSHTSVLLNETIENLKIKPDGIYVDGTLGGAGHSSHICERLS
                     ATGRLIGIDQDEAAIAAAGKRLEPFQNQVTIIRSNYCDMVPRLAEIGVTGVDGILLDL
                     GVSSYQLDNAERGFTYREDVPLDMRMDQRNALSAYEVVNDYSEENLSRILHEYGEERF
                     ARKIAHNICVSRQQAPIRTTGELIEIIKRSIPAKIRATGGHPAKRTFQAIRIEVNQEL
                     TVLSESLDGMIDLLNDGGRLCVITFHSLEDRIVKNIFRKNEHPCTCPPEFPVCVCGKK
                     SKGTVVTRKPILPGEEEMETNPRSKSAKLRVFERKV"
     gene            complement(14559..14990)
                     /gene="mraZ"
                     /locus_tag="PLN77_03245"
     CDS             complement(14559..14990)
                     /gene="mraZ"
                     /locus_tag="PLN77_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRM43330.1"
                     /translation="MFMGEYSHTIDAKGRLIIPSKFREQLGEEFVLTKGLDGCLSIYP
                     NDEWAAFEEKLRALPLTNKNARTFSRFFVAGATSCELDKQGRILVPATLREFAGLEKD
                     VVLTGNINRIEIWSKEKWNENSNYDDMDAIAESMEEMGIVI"
     gene            complement(15315..>15607)
                     /locus_tag="PLN77_03250"
     CDS             complement(15315..>15607)
                     /locus_tag="PLN77_03250"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRM43331.1"
                     /translation="VHPTFLYESLWNVGVLLFLFWFRKRKKFNGEVFLMYLIGYGLGR
                     IWIEGLRTDQLLLPVVGLPVSQLLSGCLVVGCTILVVWKRKKLSSGGETAHS"
BASE COUNT         3410 a         4527 c         3476 g         4194 t
ORIGIN      
        1 ccctccggca tattgatgcc gaaccggagc ttcacaatca cctgctcccg ctccgagagc
       61 ttgctgattg cacttccgag cacgcggtgt tccgcctcct gctcgagacc ctggtagatc
      121 acatcctcct ccgttccgag gatatcggac aagagcagtt catttccatc ccagtccaca
      181 ttgagcggct catcaatcga aacttccatg cgcgtcttgt tgtttctccg cagatacatc
      241 agaatctcgt tctcgatgca ccgggacgcg tacgtggcga gcttgatctt tttctccgga
      301 ttgaacgtgt tgatcgcctt gatgaggccg atcgtcccaa tcgagatcag atcctccaca
      361 ccgactcccg tgttgtcaaa tttctgtgca atgtagacga caaggcgcag attgtgttcg
      421 atcagctcgc tccttgccgt ctcgttttcc ggatcggaaa gcccggcgat cgcgtccgcc
      481 tccgcctggc cctccagagg cgccgggaga acgtctgcgc ctccgatata gaaaatctcc
      541 cctgtctgac gaaatacagc cggtacggac cgcggcagca tacgaagccg gaatccgttt
      601 ggcagtgcag cctgcagcat acttcattcc tcctgtcctt ccatgagatc tggatgcaaa
      661 attatttcat agcccctggg agatgaacta ttttctccgc caagcgcaat cagcggtccg
      721 cttatgtgcc tggctgtatg ttctgtcaca agaatcattt ccgagatccg gatcaccggc
      781 agaaagcccc cgccttccag tccgcgaaac ggtatgtaac ggggctgcag cgcaagaagc
      841 cgttccggct ccggcaccgt ctcgtgcctt gcaagcgccg caatccctgt ccgcatttcg
      901 tccgggaaca attcgagtat cacagtgtac ggtacgaccg agaccggctt tttggttgtg
      961 gcatcccgca aagtattgcc cgtgtcgtac agtcctttta cttttttcca tttcccattc
     1021 tgcacaagct gtaccgtaac ctgcctggcg tgttccagga gcatctcctt tcggatcttc
     1081 agcaggagga gcgttcccgc atatgcggca ctcccggaaa gaagaaaaag aagaattccc
     1141 cttcccggca tcagctcccg gaacaggcgg tatgcactgc cgatcagaaa accggacagt
     1201 atatagcctg ccattcccac cgcaagatcc cgtactgtcg tgattctgca gccagatttc
     1261 accatccctg ttgtaattcc gattccgacc agggcgagca gcgcacccgg cagataccgt
     1321 gcaagcacgg catacagcac cgcataaccg gcgccgagcg cggctgccgc aaaactcctt
     1381 cttcgcgtgg cagatcgttt cagaagaaag gaggaaaggc gcagaatagt ataattcagc
     1441 agaagattct ctgcaaagag aagatccaga aagatctcat agtgcaaaaa aacaccccct
     1501 gcgccttttg catggtctga atttctaaca gaccctatta tagacggaag ggggtgaatt
     1561 ttttgtcgtt ttcggaagaa tgaccttctt ttttgatcaa caaacttcga cacgccgcaa
     1621 cattttcggt ctgcggcgtg tcgaggcaaa ttcgttattt gcttattatt ctttgcttat
     1681 ctgcggtttc ttaagaaatc cgggatctgg atgtcctttt tcggaacatt gcttgtcatg
     1741 gtctgctgag cctgtacctg cggctgcacc tgaacctgtg gctgcggctg taccggagcg
     1801 gaagctttcg gagcagtgtg taccggagcg gctgcctgtg cagagaagct gctgctggaa
     1861 aatgcgctct ttcttgcatc tgccggctct ttgcgggata cgcttgcctg tttcgccgtt
     1921 gcatcgttga ggcctgttgc gataacggtg atcttcgctg tatcggtaac agaatcatca
     1981 tacatagcac cgaagatgat gtttgcatcc tctccagcca gattctgtac gtaacttgca
     2041 gcatcgtttg catccataag ggagatatct ccggaaatgt tgatgataac gtggcttgca
     2101 ccctcgatgg tggtctcaag cagcggactg gaaacagcct gctgtacagc ctcgagcgct
     2161 ttgtcgtcgc cctttgcctc gccgataccg atgtgggcga tacccttgtc ggtcataacg
     2221 gtctgaacat ctgcgaagtc gaggttgatg agtgccggca ggttgatcag gtctgtgatg
     2281 ccctgtacgg cctgctgcag aacctcgtct gctttcttta atgcctccgg catggttgtc
     2341 cggcggtcta cgatttcaag aagtttgtcg ttcgggatca cgatcagggt atctacgttt
     2401 tcctcgagtt tttcgattcc catcagcgca ttgctcatac gggttcttgc ctcgaatttg
     2461 aacggttttg ttacaacgcc gacagtcaga atgcccattt cttttgcaac gcctgctaca
     2521 accggtgctg cacctgttcc ggttccgccg cccataccgc aggtgacgaa taccatatca
     2581 gcgccttcca tgatctgttt gagctcttcg atgctctcct cggctgcttt ctctccaacc
     2641 tcaggttttg ctcctgcgcc cagtcccttg gtcagcttct cgccgatctg gagaatggta
     2701 ggtgccttac agagggtcag cgcctgttta tcggtgttga ttccaacgaa ttcaacgccg
     2761 ccgatggatt cctccaccat tctatttaca gcattatttc ctgcaccacc tacaccgatt
     2821 acgataatct ttgcagatga ttctgcctca tttgtcatga tctctaacaa tttcaacttc
     2881 ctccttttga ttatgaactg ctgtctatct caaaatagtc tgatattctg atggtcatac
     2941 ataaagatat aatataattt cttcggaaaa taatcaactg tttttttcgt atttcataaa
     3001 aaattcagga ttattgcgtt tttttgacgg ctttcctttc ctcttataca tctttcgaga
     3061 agacaaagct gctgtcgccg tccttgtaat cctccatatg gagttctccg gaaagaccgg
     3121 aaaggctcgg caggattgct gctacgcgcg tcatcttttc ttctaacgcc gtatcggttc
     3181 caagcagaat ccgcacggta tcatagtaat gcagaataat ttcgctgttc tcattaaatt
     3241 ccacactgtc cggcgtgata ttgtatttct ccgtcattct cgtaagtgcg agcatggtgc
     3301 ggaaaactgc cggtttttca accgggagct gctcgttcac ggtcacatag tcaaacgtaa
     3361 gtcctgttac aagcggaaca tcggaaaggg tcggctgaaa gtctgtcgtt gtcacacgtg
     3421 cacttttaat cgtatccgcc gagagcgcac cgtcggacgt actgctttcg gatgctgtgc
     3481 catcggatga tacgctttcg gatgcaagcg gctcactcgt aagggatcca tccgcactcg
     3541 ttccatcgga ggcggttccg tcggaaggct caccctccgc aggtgtttcc tgctcctcgt
     3601 ccacgcactc cagcacgatg ccgttcttgt caaaatagac cttctggcct tcgtactcga
     3661 cataaccgac cacttttttc tcatttacct ggatgcagat ggtgtcatag cttacaaacg
     3721 taaactccac ggagttcata aaaggaatat cgctcatatc aaccttcgaa tgaaacgtcg
     3781 taagaagcac cgtgtttttc atccacttgt cgtccgcgac catctctttg atttcatctt
     3841 ttgtataccg ggtgttcccc gccacctgga tattgcggat tttgaacaca ttaaaaaaca
     3901 aggcacctgc aaggccgacg ccaagcacca ccccggtcag aatccgtatt tttctgtttt
     3961 tcttcttttt tctgtttttt ttctgtctca tatttctttt tctacgataa tcttggctcc
     4021 gagttcccgg agcgtttctg gaaaccgttc atacccccgc tcaatcagtt ctgtctgccg
     4081 gattgtggaa aatccttctg cggcaagcgc cgccgatacg agtgccgcgc cgccccgaag
     4141 atcctgggaa acaacttctg caccgtgcag acggcttttt tcggccgtca gccattttcc
     4201 gcagatctgc agcgtaccgc ccattttctg cagctcctct gccgtccgga ggcggtcctc
     4261 aaatacattt tcacagatac agctctttcc ttccagcgtc atcgctgccg ccagatacag
     4321 cgactgcaga tccgtcggaa aaccgggata ttcctctgtc tccacgtacg gaacagcatg
     4381 ctgcacgttt ttgctgtctg ctaccagtgt accactcttc cttgtatatt gtccacctat
     4441 tttctgatag agggacagaa ccgcccccat ttcgtccagc ggcgcgccgg aaagcgtcag
     4501 gtgcccccgc gtcgccgcgc ccatcagaag atacgtccct gccgcgatcc ggtcacccgg
     4561 aacacgatac cggcagccgg acagcagtct ctttccctgc cttccgtaaa cggtaatctg
     4621 ctccgtcccc tcgccggtaa tctccgctcc catggcctta agaaaccggc agagatgaac
     4681 cacttccggc tcccgcgcac agtgctccag ccttgttgca ccttccgctc caacagcaaa
     4741 aagaaccgca ttttccgtcg ccccgacgct ctttttcgga aagcagagca cggctccttt
     4801 cggatgatct gcctttgccc gaatggtgcc gtcctcctcg aagacctcgg ctcccagaag
     4861 ctccattgcc ctctgatgca ggtccgtcgg ccgctttccg atccggcagc cgccggcccc
     4921 tgcaaggtat gcctcccctg tccttgcaag aagacttccc agaaacaaaa gagaggcgcg
     4981 cagtctcttc gaaagcgctc cctccacacg gcttttttcg atcttttcac agttcaggtg
     5041 gagcgcattc ctctcccacc agactgttcc gccaaggctt cttacaatct ccgccatatc
     5101 ctccacatcg gaaatcttcg ggcagccttc cagcacgacc tcctcttttg tcagaaccgc
     5161 cgccgcgatc atgggaagaa cggcattttt ggaaccctgg actgttaccg ttccgcaaag
     5221 ccgccttccg ccgagaatct gatatactcc cacctgttct ctcccatccg gcctctcttt
     5281 ttacggtatg cattacactc ccgttttgtt cccgttctgc agcgcttttc cgccttttta
     5341 atcggtgtcc ctcgagaccg acagggcaag ccccatctcg ccgagcagaa acagcacgga
     5401 ggtgccgccg taactgataa aaggcagcgt aatgccggta ttcggaatcg tatttgtcac
     5461 aaccgcgatg ttcagaatca cctgaagcgc aatatgcccc ataattccgc ccgcaatcag
     5521 tgctccgcca agatcccgcg catggatcga aatgaccatc aagcgccaga tcagcaccgc
     5581 aaacacggcg atcaccaggc aggcgccgaa cagtccgccc tcctcacaga taatcgaaaa
     5641 gatcatatca ttctgcgctt ccggcacaaa gccaagcttc tgcaggcctt ttccaagccc
     5701 gcagccaaag attctcccgc tgccgatcgc atagagtccc tgaatggtct ggaacccctt
     5761 ctcgtacgcc tccgggttgc gccagatggc aaggcgttcg aggcggtagc tctccatcat
     5821 cagaaaaacg gcaataaacc cgatgccggc gcctccgatt ccgagaaacg gcaggtagcc
     5881 cggatgtgaa ataaagatca gaaacgcaca gattccgagc aaaataatcg ccgtacttaa
     5941 gttattgctt ccgacaagcg ctgcaatcgg cagggtgcac agcaccgcct ttgtcataaa
     6001 ccagagactg tcggaccggc ctttcgtgtg ctccatcacc cagggcagga agagaatcac
     6061 cgccatcttg gcaaattccg agggctggaa cgataagggg ccaagcttta accaccgctt
     6121 cgagccgttg tactcacttc cgaaaaagag caccgccgtc gagagcgcaa gggaagccgc
     6181 ataaaaccac ggtgccagtt caaacagccg ccggtaatct atttttgcaa ccgcatacat
     6241 accgatcaca ccaagcgcac ttgcaaagag ctgcttcttg aaataataag ccgggtcatg
     6301 gaatctgacc cggccgttat acgcgctggc gctgtacaga atcacaaggc caaggccaag
     6361 cagaatccat accgtcacca gcagcgtccc atctattttt ttctcttttt ccttccgcat
     6421 ccgcgccaca cccgccgttc gctctttttc agcttatgcg ggatagtttc ctgatatgac
     6481 gtttttagtt acagttctta cgcagtaaaa cagcaagtgt ttttacagtg ctcttacgta
     6541 ctccttaaac agctcaccgc gctgctcata gctcttaaac tgatcccagc ttgcgcaggc
     6601 cggggaaaga agaaccgcat cccctgcatt tgccttgtcg tggcagatct gtacggcttc
     6661 ttttaagttg tctgccatga taatgtcgtt gaatccgacg cttcttgccg cttttgcgat
     6721 cttctctttc gtatctccga tcaaaaccag gaaacgtacc tttccatcga atgcctgaat
     6781 ccactcttcg taggtggact gcttgtcgta gccgccgccg atcagcagag tcgggcggtt
     6841 catcgcctgg atgcctttga tggccgcatc cggattcgtt cccttggaat cgttgtaata
     6901 tgccacgccg tccttctcgc agacatactc gatgcggtgc tctacccctg caaactcctg
     6961 aatgactttg cggacagttt ccatcggcac gcctgcatga agtgccatcg cagaagcaac
     7021 catgacgttt tcaaaattat gtaggccgag cagttttaag tcgtcggttc tggtcagaag
     7081 ctccgtctcg ccgttctttg caatgcggat ttcgccgtcc tgataataga tgccgtcctt
     7141 taacgcacgc agactcgaga agtacatcac ctgtactttc ttctctgcca tcagtttttc
     7201 gccgaacggg cgcaggattt cgtcctcata attcagcaca cagagatcat ccatcgtctg
     7261 attttcggtg atacactctt tcacgcggat atactcttcc atcgtgtgat ggcggttcag
     7321 atgatcctcg gtaatgtttg tgatcgcact tgccgccgga tggaactctt tgatggtttc
     7381 cagctggaag ctgctgatct cggcaaccgt tacggtatca tctgtcgtgt cgagtgctgc
     7441 ggatgtgtac ggggttccga tgtttccgac cacttttact tccggctgat atgctttcat
     7501 gatctcgccg agcagcgatg ttgtggtggt ctttccgtta gtaccggtaa tcgcaagcac
     7561 ttttccgcgg cttacctgat acgccagctc cacttcaccg atgaccggga tctctttttc
     7621 tttcatttta aggacaaccg gcagatccgt cggaacgccc gggcttaaaa cgacaagatc
     7681 aaggctgtcg agcatctcct ccgggagctc gcctaagaca acttccacgc tgtcacggct
     7741 ctctaatttt tttaagattt cttctttttc gagtttgtca tttccatcgt aaagaacgac
     7801 agaagcacct ttcttgtgca gcagatcctc cgctgcaatt ccactctttc cggttccgaa
     7861 aaccagtact ttttttcctg cgaaaatcat gtttgattac ctccatattt taaagcccca
     7921 tcaatgcgat caggcataat atcgctgtga ttgtagcaaa caccgtcaca atctgtgtct
     7981 ccgaccagcc catcagctcg aaatggtgat ggatcggcgt catcttgaag atccgttttc
     8041 ccttcgtcag tttgaagaat gacacctgca ggatcacaga gagaatctcc atcatataaa
     8101 tgaatcccac aagcggcaga aataagggga gacgtaagaa acacgccatt cccgagacga
     8161 aaccgccgag tgccagactt ccggtatcgc ccataaagac gcgggcagga aatacattga
     8221 ataccagaaa cccaagcaga ctcccgataa ccgcaagcgg ggacggggaa acaccgtttc
     8281 cgaggccgat cgatgccacg gaaaagaaaa cagcatttaa aatcgtcaca ctcgccgcca
     8341 gcccgtcaag gccgtcggta aagttgacac cgttgaccgt tccaagaatc acaaaggcga
     8401 gaaacggaat cgtaaaaatc cctgcgtcca gctctttgcc tggaaggaac ggaatgcgca
     8461 tccggaggga aattccggaa aaacgcgcca aataccacag aaagactgct gtaatcactg
     8521 cctgcagtgc cattttctgc ttcgggtaca gcccgtcgct gcggtgcagc acgactttca
     8581 gatagtcatc gagaaagccg atcaggccaa aaccaagcgt cagaaacagc accggaacca
     8641 cttccgctcc ctctttccgg aaaaggaggg aaactgcggt aaacgccgcc agaaacatca
     8701 cgccgcccat cgtcggggtt cccgcttttt tctggtggct cggcacaccc tcttcccgct
     8761 ccttctgccc cgctttgact tttcgcagaa acgggatcag aaccggcccc agcgccagac
     8821 tgatgccaaa ggaaagaagg accggcagga tcatttctct tttcatagac gcacctcttc
     8881 ctttttgtac ggattgtgta cggaacaggc atgtcaaaat attttctcca gcctattcag
     8941 tatatagctt tttgttctgt ttcgcaacct tcttttataa tccaagttac ggttcgctca
     9001 gcagtgaaac acttgctgtt tcgctgcgta agaaagtgat tcaggtgtga gcagccctcc
     9061 ttttgcgaag caaaacttta aatgagggct gcgaaggaca caattctcag gtttcctgag
     9121 aattgtgtcc aataccgaga tacggaagta cattggaaaa gatatcgcgg atcaccggcg
     9181 cgcagatcgt tccgccgtag tagacgccct gcggattgcg gataatgcaa agccccagca
     9241 ccgtcggatg ctccgccggg gtgaagccga ggaatgagga aatatagcgg ttggcgctcc
     9301 gcggaagtgt ctgcgaggtt gccgtctttc cgccgatgga atatccctcg aggtacgcat
     9361 tctttccaga cccttctgaa acgacgcttt ccagaatttc gcgcaccgtc ctggaagttt
     9421 cttccgaaag aaccccttct ttttccggaa aattcagatg ctttacaaca tttccttctt
     9481 catcgagcac atccgttgcg acatgcggcg tcaccatttt tccgccgttg acgagcgcag
     9541 agaccgtcgc cgccaggcgg atcggcgtga tctggaagga ctggccgaat gccatcgtcg
     9601 caagctcaac ctctccgatg ttttcctcct tgtgcataat cgtcgatgcc tcgcccggca
     9661 gatcgatccc cgtcttttcc atcagaccga aattccggaa gtaatcacag aacttttcca
     9721 caccgagccg cagccccact tcaatgaaca ccgggttgca cgagttctgc gcgccctgca
     9781 cgaagttttc ggctccgtgt cccgtccgct tgtggcagtg gatccggcgg tcctccacga
     9841 ttttgtagcc gggacagaaa aacgaatcgt tcggcgacac aacgccctcc gaaagtcccg
     9901 ccgacatcgt gatgatctta aaggtagatc ccggctcgta cgtatcgttg atgcaggcat
     9961 tccgccacat ctggttcagc agatcctgaa tttctttttc cgaaagtccc gttgtatcct
    10021 gctgcagcgt gaacggatcg ttgaggtcga actccggcac attgacacag gcatagattt
    10081 ctccgttctg cggattcata agaagaatcg agacgctgtc cgcctgtttt tctgccatcg
    10141 ccttttccgc tgcctgctgg cagtacgcct ggatattgac atccaggctg acctttaagt
    10201 cccttcccgc caccggctcc tggcggctct ccccgatgcc gttctgctca atcccgcgcg
    10261 catccgttgt cgtcaggatt tttccgttga ttccttttaa aatttcctca tattccacct
    10321 ccagtccgat gatgccctga ttatcgcctc cggtgaagcc gataacgcgc gacgccagct
    10381 tccctttggg gtaataccgc ttgaaatcct catccacttt tacccccgcg tatccgtacg
    10441 accggatggt atcacccgtc tccttgtcca cattcgtctt gacacgctcg attgaagacc
    10501 gcttctccac gcgtttccgc gccgtctcct ccgaaattcc aagctctttt accagcatcg
    10561 caatcaccgc ctccggctcc tcgatctggc tgtggatcac ggaaatcgta cagaccgtgc
    10621 ggtttgccgc gagaacttcc ccgtttgcat caagaatccg gccgcgcgcc gccttgatcc
    10681 cgcgctcccg ctcgtgcaga tcatccgcct tctcggaata atagacggac cggaacacac
    10741 acagatacac cagccgcccg gcaagtccga ccatcattcc catgcagagt acccacagaa
    10801 gaaccgtctt tttccggtga tacgttttgt ttttacgcat agaatacccc atagccgaat
    10861 ccattatgat aacactatat ggaaactgct atggggttat gtcttttgtt ttttatgttt
    10921 ttttactctt cattgatgag atccagctcg gcgtttgtca gaccgccgtt ctcgatattg
    10981 ctgttttcag cactgttttc cgtatcgtct tcctgtaccg gtgccgctac gtttggatca
    11041 tccgttccgg tctcggagcc ttcgcccgtc acaccgtccg tatcatccgg gaatacgttc
    11101 aaatacggca gcagttctgt gtagatctct tttacgatct cctgtgccag cacactgttc
    11161 gcctgatttt ctacatccgg ctcgtccaca atcgcgtaaa caacagcttt cgggttttct
    11221 accggtgcaa atccgataaa ggaaacgaga tattttccat tgcctcgcgg atacttctgt
    11281 gcagtacctg tttttcctcc catcgtgtaa ccggcaacct ttgcactctg gccgcttcca
    11341 tcggtcacga ctccctggag gaaacttctc attttatcgc tgatttccgc ggaaaccgtc
    11401 tgacgcacca gattcggctc attgctctca acaacggctc ctgtgctgtc cgtgatcgtc
    11461 ttcatcacat gcggctggta ataattgcct ccgttgatca gagaggaaaa cgcagctgcc
    11521 tgctgtacca tcgtcacggt atagccctgt ccgaatgtac tcgttgcaag ctcggtcggt
    11581 cccatggtat cctccgtgtg aaccaggccg tacgcctcac ccggcaggtc aattcctgtc
    11641 agactgccga aaccgaaaat atcctgatag cggagaaatt ccgctgctcc catcttctgt
    11701 ccgatctgca tcataccagc gttgcaggag tttttcagta catcggccgg tgtctgtacg
    11761 ccatgtgttc ccggataagc tccgcagcgg atcatctgcc ctgcaatcgt ctcaaacccc
    11821 tcacagtcaa acagggtatc atcatcgatt acatcgaggg aatatgccgc cgccacgttc
    11881 atcggttttg ccgtggaacc tggctcatat gcatcggaaa tacagtagtt tttccagatg
    11941 gcactcaaag cctccattgt ctcgttatcg ttcatcgcat caatttcttc ctgcgaatag
    12001 aacggtgtca gatctcttgg attgttcagg tcataccagt cggaagaatc cataccaaga
    12061 atctctccat tgttcggatc catcacgaca acgccgatat tctttgccgc ttttgtctga
    12121 cggttcgtct cagtgtccga ttcgtccgcg ccgttctgga catgaatccg ctcattgtaa
    12181 ttctcgatcg ccgtgcggat gatcttctga atatttacat caatcgtcgt aaccacgttt
    12241 ttgcccggct gcgcctcgat gatcgtctgt tccatatccg catcttcgtt gtaatagccg
    12301 aactggcggc cattcacgcc atttaagata ctggaataat acccttcgat tccccagtct
    12361 gccgtgtctc ctgaatacgt aaagccgatc agatcgcagg caagtgaatt taacggatac
    12421 gttctgtggt aggattcctc gaaccagacg ccgcggatgt tggcacggta tgctttttcc
    12481 gcctgctcgt tctcatcgag ctccttgttc tttttgtccg cgtactcgtc cacataggtc
    12541 tcaaacgctt ttttcgcgtc catcgagacc tctctggcga gaacctggta ctggctttct
    12601 tttgtcgttt cgccggtcag acgatcccgg atatccgtct cgtcgagtcc gaagtattca
    12661 accagtgctg ctacggtcgg atcgacgtac ggatgggtat cgttcttttc ttcatctttt
    12721 accgtcgtat tggcaagttt acagtcgaga ataacgttgt aaaccttctc actcgttgcc
    12781 agaatcgttc cgttccggtc ggtgatactt ccccgctgga atggaattgt cctgctgtcg
    12841 taggactgct gcgtctggct taaaacctgt ctttcgtatt tttctccctt tgtggcattc
    12901 acatacgtaa tgtcaatcgc taaagcaaca aaaaccagca tcactcccaa aaagagtgct
    12961 accagttttc tgctcatacg cccacttgtc ttccgtctcc ggaatgcccc gcttcgtttt
    13021 ttattttttt tctgtttact cacgttttca cctatttcgc atccggaaca tcctgatact
    13081 gtctcacata gctgttttct gaatcatcga aataaattac ctgggactca tctgcatatt
    13141 tcatgccgag ccggttctgt gctgcttctt tgactgcctc aagatcttcg gaggcaaccg
    13201 ttgtattgta ataggcgtca ttgtccgcct tcagatcgct caattctttt tctttgctgg
    13261 taatgtcttc cgcctgcgtg gtaaccacgg tctgcaactt gacatacgca acacacgccg
    13321 cgatacacag tgtgcagaca atggcaagaa aaacagcgta gcccgcgctg atctgtgtcg
    13381 cccgtgcccg gtttcttctt gtctggctgc tcacacccga tttcagaggc cgtgcttctt
    13441 ttggtgcttc ctgcggctgt gctgcaagac gccgtaccgc acttccgtcc tcaaacgtat
    13501 ctggcgtata acgtttcgtt ctatctgtcg aatttaccct tccatctgcc cgtctggcca
    13561 taacttctct gtctccttcg tgccgagccg tcacactttt cgttcaaaga cgcgcagttt
    13621 tgcgctcttg gaacggggat ttgtttccat ttcctcttct cccggcaaga tcggtttccg
    13681 agttactacg gttcccttgg acttctttcc gcatacacag accggaaatt ccggcggaca
    13741 cgtacatgga tgctcgtttt ttctgaaaat gttttttacg atacggtctt ccagagaatg
    13801 aaaggtgatg acacaaagtc ttccaccgtc atttaagaga tcaatcatgc cgtccagtga
    13861 ctccgacaga acggtaagtt cctggttcac ttcgatacgg attgcctgaa aggttctctt
    13921 ggccggatgg ccgcccgtcg cccttatttt tgccggaatc gagcgcttga tgatttcgat
    13981 taactcccca gttgttcgaa tcggcgcctg ctgccggctt acgcatatat tgtgtgcaat
    14041 ttttcttgca aaacgctctt ctccatattc atggagaata cgcgaaagat tctcttcact
    14101 gtaatcgttc acgacttcgt acgcggaaag agcatttctc tgatccatcc gcatatccag
    14161 cggaacatcc tcacggtatg tgaatccacg ctctgcatta tccagctgat aggatgaaac
    14221 tccaagatcc agcaggattc cgtctacccc ggtgactccg atttcggcaa gacgcggaac
    14281 catatcgcaa taattgctgc ggataatcgt gacctggttc tgaaatggtt ccaggcgttt
    14341 acccgcagca gctatcgcag cttcgtcctg atctatgcct attaatctcc ccgtggcaga
    14401 taagcgctca catatatgac tggaatgtcc agcgcctccc aacgtcccgt ccacgtagat
    14461 tccgtccggt tttatcttaa ggttttcaat ggtttcattc agtaatactg atgtatgcga
    14521 aaattccatg gtcatctcct gtatcccaac gaaacagatc agatcacaat gcccatctct
    14581 tccattgatt cggcgatggc atccatatca tcataattgc tgttttcgtt ccatttttcc
    14641 ttgctccaga tttcaatccg gttgatattt ccggtaagga ctacatcttt ttccagacca
    14701 gcaaattccc gcagcgttgc cggtacgaga atcctcccct gcttgtccag ttcacatgac
    14761 gtagcacctg ctacaaagaa acgggaaaaa gttctggcgt ttttgtttgt aagtggtaag
    14821 gctctcagct tctcttcaaa ggcagcccat tcgtcgtttg ggtaaatgga aaggcatccg
    14881 tccaatccct tcgtcagtac gaactcttct cccagctgtt ctctgaactt ggacggtatg
    14941 atcaatcttc ccttcgcgtc aatcgtatga ctgtactctc ccatgaacat aatgaaaacc
    15001 ctcttatcac ctgaaatggc ggtctcccca cgcaccactt catgtatcgc gccgcagccg
    15061 gactgccaca tccgctccac aacgcaccac ttttcaccac tttctaccac ttatgttttt
    15121 attctatatc aactcaggga aaatagcaag gtcttttttc tatttcattt ttaagatttt
    15181 ccccaaaatg caacattttt atgcgcattt tccgcctttt ttataccaat tttgtatcaa
    15241 aaaaagagaa aacctttttt accggttttc cctttttctg tatttttatt ctgttttttt
    15301 cactggtttt atccttaaga atgggctgtc tccccaccgg atgataattt ttttcgtttc
    15361 cataccacca ggatggtaca tcccaccaca agacaccccg aaaggagctg ggaaaccgga
    15421 agtcccacca ccggaagcag aagctggtcg gtgcggagtc cctcgatcca gattcttcca
    15481 aggccgtatc ctatcagata catcaggaac acttcgccgt taaatttttt tcttttccga
    15541 aaccagaaaa gaaaaagcag gacacccaca ttccataaac tttcatataa aaaggtggga
    15601 tgcacct
//
LOCUS       DAOMUF010000030        15138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_826_length_15138_cov_3.298946, whole genome shotgun sequence.
ACCESSION   DAOMUF010000030 DAOMUF010000000
VERSION     DAOMUF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_826_length_15138_cov_3.298946"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLN77_03255"
     CDS             <1..596
                     /locus_tag="PLN77_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43332.1"
                     /translation="ARAELCLISRQEALNAWKVHLDQDYLVLSENYVWEENGELVVTG
                     SGKTMIAVYPAVEKGIVDFKECGKRGNFTLYERIYKAQEPEAELVCKEQDKEKAVYEL
                     KLAYPGEKNYHDAFAFLTWYGNRMEVFDGEEKINDYFYTGQEALLSLGYFEFPEKLKL
                     VVYPLHPGDPIFLEKQPDAADGCACKIEKLHVETIFR"
     gene            617..2611
                     /locus_tag="PLN77_03260"
     CDS             617..2611
                     /locus_tag="PLN77_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRM43333.1"
                     /translation="MNQSGFIYGGDYNPEQWLAMPEILEQDIEYMKEAHINEVTLGVF
                     SWSVLEPEEGVYHLEWLEKIMDQLYKNEISVILSTPSAARPKWLADKYPEVLRVNADR
                     TKNLFGGRHNHCYTSPVYREKVGKIDRLLSEKFGKHPAVKMWHISNELSGECHCPLCQ
                     KAFQGWLKKKYETIDALNMSWNTTFWSHSYQNFEQIESPSPKGEMGLHGLNLDWKRFV
                     TDQTADFVAWEKAAIRAGGSELPVTINMMYDFQGLNYHKFKDLIDVVSWDNYPTWHKE
                     AEEVTALDAALQHDFMRSLMKKPFLLMESCPTSTNWQSVSKLKRPGLLKAASLQAVAH
                     GSDSVQYFQIRQSRGASEKFHGAVIDHYGGKDTRVFREVTETGEALLDLAQIAGSTTK
                     AQAAILHDCESRWAMEDAQGPRNMGLHYMNTVKKVYGGLRKLGVNVDFLDMEESLSGY
                     RIVFAPMLYLFRAGIEEKIRKFVADGGTFVMTYWSGVVDENDLCFLGGTPHGLMDVFG
                     LRSTELDALYDQDVNAGVGEGKTYEIRNFCDLVKTNGAETLLAYRDDFYAGYPALTKN
                     VFGEGEAYYVCADFEQAFYDDFCRKLAEEKGLERAAAEIPEGLDVATREDEKYKYLFV
                     QNYGAEPAAFPMDTETYQPMTGEYDGVVRRFETVVFRKEK"
     gene            complement(2681..3598)
                     /locus_tag="PLN77_03265"
     CDS             complement(2681..3598)
                     /locus_tag="PLN77_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM43334.1"
                     /translation="MKLAVISDLHGNYYALAKVLAFLRSQEIDGIIGLGDFVTDGPFP
                     QRMMRVLREMQEEFPCYLVRGNREEYLLENLRHPQNWKAGSSTSGLLDYTFRNLTGQD
                     LEFFEHLPTDGVLVGKINESGRLIPVFVPQEEITPDTCRESLFPALRLCHGAPGELRG
                     NFGLHPELLLSHLKNLDAAILLGGHSHKQEQREYAEKTYLNPGSLGLALDGVGGHAHF
                     AILTLENSTWQIECFQIPYDLEGYLAEFDRAGLETHGSVLARSLKRTLTCGVNYFYLT
                     VKRVRELADALALTDLDEEVWKKAEQELK"
     gene            3802..4446
                     /locus_tag="PLN77_03270"
     CDS             3802..4446
                     /locus_tag="PLN77_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 family protein"
                     /protein_id="HRM43335.1"
                     /translation="MINREDMLELTRRMTPARTSFVRMAGCYTDSDGEYDGSFNIHFL
                     KLSGSEKARNLAIAKKIPFAESNEKLREYRFPETSQGPGSIWQMLMALRECGLKNDAL
                     LETFYDILIEGLQIHGAYAIYMFYDRYDIPAKASDKERLGESEEMFPYLICAVCPLVG
                     EYEPGNPICGFLFPAFVDRSGDLERVDIYAERAAWGDRMASILGAKGRKFRCGL"
     gene            complement(4578..5006)
                     /locus_tag="PLN77_03275"
     CDS             complement(4578..5006)
                     /locus_tag="PLN77_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43336.1"
                     /translation="MRKIRRVCMTLALAVLLAGMTACGNNTTNDTGNGNTNTESDMGQ
                     TDNRNDVNTTEMPSNDNVNDVTEGTDGNANGTNGTNTEDGNTLGDDVGDAAEDVTRGV
                     GEGVEDVGRAVDDVVSGGDNETQNPQENGATTQNGAGMAQ"
     gene            5174..5407
                     /locus_tag="PLN77_03280"
     CDS             5174..5407
                     /locus_tag="PLN77_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43337.1"
                     /translation="MEVNPANRREKIISLTETGKQYARELVLPLFQSEEEAAAQFTEQ
                     EMTEVIRMQEKFADALAKSMEEKVSIVHNLSAS"
     gene            5445..6668
                     /locus_tag="PLN77_03285"
     CDS             5445..6668
                     /locus_tag="PLN77_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRM43338.1"
                     /translation="MKEELKKSLRTYGALLLLGVTGVPLGAAVGLIEALFCRLLLKVT
                     AIRVAHLFWCLPFLAMAGLVIVAVNQKFGGRGKGGMSLIFDVAYEEETELPLRMIPLA
                     MLSTALTHLFGGSAGREGVAVQIGAVFSYFAGKKLPFKNAPGIFLVTGMAAGFAGMFG
                     TPIAAVIFAVEVLAAGEMRYEALLPAFTASFSASAVASLLGIRKENLAVHISESLTPF
                     LLVRLITLGIIFGITGGLFAWCLKHTKKYLGEKIKNPLVRVFSIGVILSILFLLLYRG
                     RYCGFGTNLVEAVFHGEKVYAYDWALKFILTILTLSAGFQGGEVTPLFTIGATLGAVL
                     GNLFGIPVELSAALGYAAVFGGGTNTLLAAIFIGGEIFGYDYLPYFFIVCTAAYVFNR
                     NSSIYSRQKLTKYSC"
     gene            6884..7771
                     /locus_tag="PLN77_03290"
     CDS             6884..7771
                     /locus_tag="PLN77_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM43339.1"
                     /translation="MITYDYYRIFYFVAQYHSFTKAAEILKNNQPNITRCMNNLESEL
                     GCKLFIRSHQGVTLTAEGEKVYERVAVAYEQLRTVEEELALERSLESGLITIGASETA
                     LRLMLLSRLEHFHTRYPHVRLRISNHSTPQAIQALENGLVDFAVVTSPVEIRKPLHKI
                     PIFPFQEILIGGSKYASLSAEPHHLGELSEYPFISLAKDTGTRELYAEYFLNHSLAFH
                     PDMEAATTDQILPMVEYNLGIGFYPEELARDALKSRTVCRIPLIEEAPKREICLIINP
                     RQHQNAAAKELIGELLERV"
     gene            complement(7871..8152)
                     /locus_tag="PLN77_03295"
     CDS             complement(7871..8152)
                     /locus_tag="PLN77_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43340.1"
                     /translation="MEVFLECCMTLFFSAAMLLLIVRLAKGEEKNMMFARVFSLASNV
                     GFWIGIVRIAVLNRSGFLMFFYITGAMLSYTAMVFAMPHKKEQISRQQA"
     gene            complement(8188..8880)
                     /locus_tag="PLN77_03300"
     CDS             complement(8188..8880)
                     /locus_tag="PLN77_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HRM43341.1"
                     /translation="MEKKASFVGRLLSENQMSEAFINSMFLALSGGFQDAYTYNCRDE
                     VFSNAQTGNVVLMSQNFLEGNFTAGLRYLFPILFFAMGVFLAENFQEHYRYAKRLHWR
                     QVLLAAEIVILAIVGFIPSSYNMLAAMLVSFACAIQVQTFRKVGGYSYASTMCIGNLR
                     SGTAALSMYLRDKKKEQLRQAGYYFGVILAFAIGAGIGGVFSMLYGIHVIWISCGLLL
                     VSFLLMSLEKYR"
     gene            complement(9130..11028)
                     /locus_tag="PLN77_03305"
     CDS             complement(9130..11028)
                     /locus_tag="PLN77_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43342.1"
                     /translation="MHNYYPILWVGAIIGVISTLLIVAAFVVKDGEKETGFERNMKDS
                     EIMQRLMDYAKPFYRQFIVVGFLMLFSIAYDIISPLIVGKIEELVVGKFALNTLFLWV
                     AGYAAILLVSMACTYFQAVILQKTGQKIISNMREDLFVHIERLSHEQLNEIPVGKLVT
                     RTTNDTNAISLMFTNLLVNLLKNFFVIIGILIAMLFLNYELTLMVLCFVPFIVLFSII
                     FRKFSRRAHRKVKDCTTDINTYLSENLSGMKITQIFNREDAKMREFTDKSNALGRAQQ
                     EQIFVFGIFRPLVYMLYISSVLCLLYLGGKGYLEGTSFLGQTLTSGMIVSFYMYISKF
                     FNPIQNLAEQFNWLQSALASAEKVFSIMDLEPKMQDAPDAIELTDIRGEIEFRDVWFS
                     YIPDEWVLKGVSFHVNPKETIAFVGSTGSGKSTILSLICRNYEFQKGEILIDGIDIRK
                     IKIDSLRKKFGQMLQDVFLFSGTIRSNIILREEGISDEEIMDVCHYVNADQFINKLDH
                     GLDEPVRERGNNFSAGQRQLLSFARTIIHKPSVMILDEATANIDTETELLIQDSMEKM
                     KNIGTMLIVAHRLSTIQHADEIIVLSHGKIVEHGTHQELLAAQGRYYQLYTLQYHREQ
                     SEKQRGRK"
     gene            complement(11031..12803)
                     /locus_tag="PLN77_03310"
     CDS             complement(11031..12803)
                     /locus_tag="PLN77_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43343.1"
                     /translation="MIFGKYINKYYLKNAPILILGLAALILVDYFQLFIPELYRMVIN
                     GMNGDPVLVNGQAAVFDMEFLLDQVCRPMIITIIVMVVGRFLWRVCFFGSAIRVVTDL
                     RTCMFAHSKELSQEYYQVNKVGNLMSLYTNDLDTVQECFGDGILMFCDALFLGLLAVI
                     KMWRMNHFLTGLSMIPMAFLMAAGTTLGKYLMKKWEERQAAFSELSDFAQENYSGLAV
                     IKAFVKETKELMMFRRLNRENEDINVEYTKISTLLNIMVTLFVESVICVILGYGGYLV
                     YCGSFDAGQLVEYIGYFSAVVWPIMAVSMLIEKTSRGKASLNRITELLDAKVDVKDRG
                     GVQDLPSIHGKIEFRNLTFRYPDGEYDVLKNVSFTIEPGESVGIVGKTGSGKTTIVDL
                     LLRTYNVPDGTLFIDDHDVNTVSIHSVREGCAYVPQDNFLFSDTISGNISFAFDDENQ
                     EAIEDAAMMSDVHDNIAEFKEGYRTVLGERGVTVSGGQKQRISIARALMKHAPILILD
                     DSVSAVDTKTERTILDNLKKREGLTTILIAHRISTVEQMDKIVFVEDGEVHAVGKHED
                     LYRTCTAYRKMVDLQKLEEEAGEE"
     gene            complement(13134..14660)
                     /locus_tag="PLN77_03315"
     CDS             complement(13134..14660)
                     /locus_tag="PLN77_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRM43344.1"
                     /translation="MNGSMFIGTWWALVPPLLAILLAFVTKEVYSSLFIGSAVGVLLY
                     TGFHPWDAFTSFFEILRNSMNINILIFDILLGMVVVLMAKSGGSAAYGKWAGTKIKTK
                     RSALLATMGLGVLIFVDDYFNCLTVGSVMRPVTDSHKVSRAKLAYIIDSTAAPVCIIA
                     PISSWAAAVNSYVPADAGISGFQLFLRTIPYNLYAILTIAMVFYICYTGFDFGQMKLH
                     EDNAAKGDLFTTGGEEFEQVSEQEVNPNGKVIDLVLPVAVLIVSAIGAMVYTGFLGGA
                     DNVISAFAGCDAETSLIFASVVTILFMMALYLPRKVITFKSFMDSLSEGFKLMVPAVT
                     ILVFAWTLKGVGDAMGLAQFVGSVVGDHASASIFIPVVLFAVAVFLSFSTGTSWGTFA
                     ILVPIATGMFAAGTNLEMMIISVSAVLAGAVCGDHISPISDTTVMSSAGAQSNHLNHV
                     STQMQYAAVTACVCLVGYLIAAVTRTWWITLGVSLLLLLAVLTGMRRFCAKKAENGKK
                     "
     gene            complement(14695..>15138)
                     /locus_tag="PLN77_03320"
     CDS             complement(14695..>15138)
                     /locus_tag="PLN77_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRM43345.1"
                     /translation="MACPSQVDKIRDTSVLEGTPIDQVFIGSCTNGRLEDLQAAAEVL
                     KGKKVADYVKLIVTPASRKIYLQASKMGILDTLAEAGAMITHPGCGLCCGRAGGILSD
                     GERVVATNNRNFLGRMGTSKVEIYLASPKTAAACAVAGKIVTPEV"
BASE COUNT         3752 a         3789 c         3871 g         3726 t
ORIGIN      
        1 atgcgcgcgc agaactctgc ctgatttccc ggcaggaggc gctgaatgca tggaaggtgc
       61 atctggatca ggattatctc gttctttccg aaaattatgt ctgggaagaa aacggtgagc
      121 ttgttgtgac aggcagtgga aaaaccatga tcgctgtgta tccggcagtg gaaaaaggca
      181 ttgtggattt caaagagtgt ggaaaaagag gaaactttac gctgtatgag cgtatttaca
      241 aagcacagga gccggaggca gagcttgtct gcaaagagca ggataaagag aaagcagtat
      301 acgaactgaa gcttgcttat ccgggcgaaa aaaattatca cgatgcgttt gcttttctga
      361 cctggtacgg aaaccgtatg gaagtgtttg atggggaaga gaaaatcaac gattatttct
      421 ataccggtca ggaggccctg ttaagccttg gctattttga gtttccggag aaactgaaac
      481 tcgttgtcta tcctctgcat ccgggtgatc cgatttttct ggaaaagcag ccggatgcag
      541 cggatggatg tgcctgcaaa attgagaagc tgcacgtaga gacgatattt cgatgaacag
      601 agaaaggaaa acgaacatga accagagtgg atttatctat ggaggggatt ataacccgga
      661 gcagtggctt gcgatgccgg aaattctgga gcaggatatc gaatatatga aggaggcgca
      721 tatcaacgag gtgacgcttg gtgtcttttc ctggtctgtg ctcgagccgg aggagggtgt
      781 ataccacctg gaatggctgg aaaagatcat ggatcagctg tataagaatg agatttcagt
      841 aattctctcg acaccgtctg cagcacgccc gaagtggctt gcagacaaat acccggaggt
      901 actgcgcgta aacgcggacc gcacgaaaaa tctgttcggc ggacgccata atcactgtta
      961 tacttcgccg gtttaccggg aaaaggtggg gaaaattgat cgcctgctga gcgagaagtt
     1021 tggaaagcac ccggcggtaa aaatgtggca catttcgaat gaactgagtg gagaatgcca
     1081 ttgcccgctc tgccagaagg catttcaggg gtggctgaag aaaaaatatg agacgattga
     1141 tgcattgaat atgtcgtgga atacaacgtt ctggagccat tcgtatcaga attttgagca
     1201 gattgagagt ccgtccccaa agggtgagat ggggcttcac ggactgaatc ttgactggaa
     1261 acggtttgtg acagatcaga cagcagactt tgttgcctgg gaaaaagcag cgatccgcgc
     1321 gggcggttcc gaacttccgg tgacaatcaa catgatgtat gatttccagg gactgaatta
     1381 tcataagttc aaggatctga ttgatgtggt gtcatgggat aattatccga cctggcacaa
     1441 ggaagcagaa gaggtgactg cgcttgatgc ggcactgcag cacgatttta tgcggagttt
     1501 gatgaaaaaa ccgttccttc tgatggagtc ctgcccgaca agcacgaact ggcagagtgt
     1561 cagcaagctg aaacggcctg ggcttttaaa ggcggcatcg ctgcaggcag tggcgcatgg
     1621 ttccgacagt gtccagtatt tccagatccg ccagagccgc ggtgcgagcg agaagttcca
     1681 cggggcggtc attgaccact acggcggaaa ggatacgcgc gtgttccgtg aggtgacaga
     1741 gacgggcgag gcgcttttgg atctggcgca gattgcggga agcacgacga aggcacaggc
     1801 tgcaattttg catgactgcg aaagccgctg ggccatggag gatgcgcagg gaccacggaa
     1861 tatgggactg cattacatga ataccgtgaa gaaagtatat ggcggtcttc ggaaactggg
     1921 tgtgaatgtg gatttccttg atatggagga gagtctttcc ggctaccgga ttgtctttgc
     1981 accgatgctg tatctgtttc gggccggaat tgaggaaaaa atccggaaat ttgtggcaga
     2041 cggcggcacc tttgtgatga catactggtc gggagttgtg gatgaaaacg acctctgttt
     2101 cctcggcgga accccgcacg gactgatgga tgtattcggc ctgcggagca ccgagctgga
     2161 cgcattgtat gatcaggatg tgaatgcggg tgtgggagaa ggaaagacat atgaaatccg
     2221 aaatttctgc gacctggtaa aaacgaacgg tgccgaaacg ctgctggcat accgtgacga
     2281 tttctatgcg ggatatccgg cactgacgaa aaatgtattt ggagagggcg aggcgtatta
     2341 tgtctgcgca gattttgaac aggcatttta cgatgacttc tgccggaaat tggcagaaga
     2401 aaaaggcctg gaacgcgcgg cagccgaaat cccggaaggg ctcgatgttg caacaaggga
     2461 ggatgagaaa tacaagtatc tgtttgtaca gaactacgga gctgaaccgg ctgcatttcc
     2521 gatggatacg gaaacatacc agccgatgac gggagagtat gatggagtgg tcagaagatt
     2581 tgagacggtt gtgtttcgaa aagaaaaata gaagttaggg aatagaaaca gacggcaggc
     2641 tgtgtgtgag tacacagcct gccgtctttt gaatggacgg ttatttcagt tcctgttccg
     2701 cctttttcca gacctcttcg tccagatcgg tcagtgccag tgcatcggcg agttcacgca
     2761 ctcgttttac ggtgagataa aaataattca cgccgcaggt caatgtccgt tttaaggaac
     2821 gggcaaggac actgccatgc gtttccagcc cggcgcggtc gaattcggcg aggtagcctt
     2881 cgagatcgta gggaatctga aaacattcga tctgccatgt gctgttttcc agcgtcagaa
     2941 tcgcgaaatg ggcatggccg ccgactccat caagcgcaag cccaagggaa ccgggattca
     3001 ggtatgtttt ttcggcatat tctctttgct cctgcttgtg gctgtgcccg ccgagcagga
     3061 ttgcggcatc gagatttttc agatgggaca gcaaaagctc cggatgcaga ccgaaattcc
     3121 cacggagttc tccgggcgcg ccgtgacaga gacgaagtgc cgggaagaga gattcgcggc
     3181 aggtatccgg tgtgatttct tcctgcggaa caaaaacagg aatcagccgg cctgattcat
     3241 ttatttttcc gaccagaaca ccgtctgtcg gcagatgttc gaaaaattcc agatcctgtc
     3301 cggtaagatt ccgaaacgta taatccaaaa gtccgcttgt cgaggaacct gccttccagt
     3361 tctgcggatg ccggagattt tccagcagat attcttccct gtttccccgc accagatagc
     3421 agggaaactc ctcctgcatt tcgcgaagca ctctcatcat ccgttgcgga aaaggcccgt
     3481 ctgttacaaa atctcccagc ccgatgatac cgtcgatctc ctgactgcga agaaatgcca
     3541 gcacctttgc gagcgcataa taattcccgt gaagatcact gattactgct aatttcatgt
     3601 gtcgtccttt ctgtgatatt gagtcaaaag gtgttttgtc ccaacatctc tctatatcaa
     3661 aattataaaa tgcgagatac tgtttcgatt ttatcagatg gagagaaaga acgcaattta
     3721 aaaacctgct gacagaaaaa gcatgttatg ataagatcaa aggcagagag tttcgaatat
     3781 ctttactgtc ggagggaatg tatgattaat cgggaagaca tgctggagct gacgaggagg
     3841 atgacgccgg cgcggacttc gtttgtgcgg atggcgggtt gctatacgga ttcggatggg
     3901 gagtatgacg gcagtttcaa cattcatttt ctgaaactgt ccggttcgga aaaggccagg
     3961 aatctggcga ttgccaagaa aattccgttt gcggaatcga atgagaagct gcgggaatac
     4021 cgttttccgg aaacatccca ggggccgggc agtatctggc agatgctgat ggcgctgcgg
     4081 gaatgcggac tgaagaacga tgcgctgttg gaaacgttct atgatatcct gatcgaagga
     4141 ttgcagattc acggggctta tgccatttac atgttttatg accggtatga tattcctgca
     4201 aaggcttccg ataaagagcg cctgggggaa tccgaggaga tgttccccta tctgatctgc
     4261 gctgtctgcc cgctggttgg agaatacgag ccgggaaatc ctatctgtgg attcctgttc
     4321 cctgcctttg tggaccggag cggagatctg gaacgggttg atatttatgc agagcgtgca
     4381 gcgtggggcg atcggatggc cagcattctt ggcgcgaagg gcagaaaatt cagatgcggt
     4441 ctgtaaaaga aaaaatcaaa attgacggat caaaccaggt gctgatgtgc ctggcagtaa
     4501 aaaaacttcc cggcgaacct gcatgtatcg ttcgccaagg gaagtttttt agatacggga
     4561 gcagcagaag tgcatattta ctgtgccatc cccgctccat tctgtgtagt tgctccgttt
     4621 tcctgcggat tctgcgtctc attgtcgccg ccgcttacca catcgtccac cgcgcggccg
     4681 acatcctcaa cgccctcacc gacaccacgg gtcacatctt cagcggcgtc gccgacatca
     4741 tccccaaggg tatttccatc ctcggtattt gtaccattcg taccgttggc attcccatcg
     4801 gtgccctccg tcacatcatt tacattgtcg tttgacggca tctctgtcgt attgacatcg
     4861 tttctgttat ccgtctgtcc catgtcgctc tccgtgttgg tattgccatt tccggtgtca
     4921 ttcgtggtgt tgtttccaca ggccgtcatc ccggccagta ataccgccaa tgccagcgtc
     4981 atacacactc ttctgatttt tctcatcttc aaaatctcct ttccctaaca gtgtctggac
     5041 agttgctagt atttgaaaaa tgccggaaaa tatacggaaa aaagaggctt ttggaaatct
     5101 tgacaaagga agaaaaaaac actaaaacaa aaagtacgaa aacggactaa aaacgaaaac
     5161 aggatatatc gaaatggaag tcaatccggc aaaccggcgg gaaaaaatca tcagtctgac
     5221 tgaaacggga aaacagtatg cgagagagct ggttcttccg ctgtttcagt ccgaggagga
     5281 agctgcggcg cagtttacgg agcaggagat gacagaagta atccggatgc aggaaaaatt
     5341 tgccgatgca ctggcaaaga gtatggagga aaaggtatcc atagttcaca atctgtccgc
     5401 ttcgtgatat gataacagca gaattcgaaa aggagagaaa gagaatgaaa gaagaactga
     5461 agaaatccct tcgcacctac ggcgccctgc tgctgctcgg tgtgaccggc gttccgctcg
     5521 gtgcggcagt cggtctgatt gaggcgctgt tctgccgtct gcttttgaaa gtcacggcta
     5581 ttcgcgtggc acatcttttc tggtgtctgc cgtttctcgc tatggcaggt cttgtgatcg
     5641 tggcagtcaa tcagaaattc ggtggcaggg gaaaaggagg catgagtctg atttttgatg
     5701 tagcgtatga ggaggagaca gagcttccgc ttcgcatgat tccgctggca atgctctcaa
     5761 cggcgttgac gcatctgttc ggtggaagtg ccggccgcga gggcgttgcc gttcagatcg
     5821 gtgctgtgtt ttcctatttt gccggcaaaa aattaccgtt taaaaacgcg cccggtatct
     5881 ttctggtaac cggtatggcc gccggctttg cgggcatgtt cggaacgccg attgcagctg
     5941 tgatctttgc ggtagaggtg ctcgctgcgg gtgaaatgcg gtacgaggca ctgcttccgg
     6001 cgtttaccgc atctttctct gcgagcgctg tagcttccct gcttggaatc cgcaaagaaa
     6061 atctggctgt ccacatcagc gaaagcctga caccgttcct gctggtgcgt ctgattacac
     6121 ttggtattat ttttggaatc acaggcggcc tcttcgcctg gtgcttaaag cacacgaaaa
     6181 aatatctcgg cgaaaaaatc aaaaatccgc tggtacgtgt tttctccatc ggtgttatcc
     6241 ttagcattct gttcctgctt ctgtaccgcg gacggtattg cggctttgga acgaatctgg
     6301 tggaagcggt atttcatggg gaaaaggtat atgcatatga ctgggcgctg aaatttatcc
     6361 tgacaattct gacgctgtcc gccgggtttc agggcggcga ggtaacaccg ctgtttacca
     6421 tcggagcaac cctgggagca gtccttggca atctgttcgg tattccggtg gagctttccg
     6481 cggcgcttgg ctatgcggcg gttttcggtg gcggaaccaa taccttactg gcggctattt
     6541 tcatcggcgg cgagattttt ggctacgatt atctgccata tttctttatt gtctgcaccg
     6601 cggcctacgt attcaaccgc aacagctcga tctattcccg ccagaaactg acgaaataca
     6661 gctgctgata acagaaacgt cacaaagtta tcacacaccg ttacgtgtta cagttcactg
     6721 gtgcccagcg aactgtaaca ttcgcaaccc tcatttaaag ttttgcttcg caaaaggagg
     6781 gttgctcaca cctgaaacat gttcttacgc agcgaaacag caagtgtttc actgctgagt
     6841 gaacagtaac ggatttttct tttttcatcc ttcggaggct tttatgatta catatgatta
     6901 ttatcgcatt ttttacttcg tagcacaata tcacagcttt acaaaagccg cggaaattct
     6961 gaaaaacaac cagcccaaca tcacacggtg catgaacaat ctggaaagtg agcttggctg
     7021 caaacttttt atccgttccc accagggtgt cacactgaca gcggagggcg aaaaggtgta
     7081 tgagcgtgtg gccgttgcct acgaacagct ccgcacggtt gaggaggagc tggctcttga
     7141 gcggtctctt gagagcggcc ttatcacaat cggtgcgagc gagacggcac ttcgtctgat
     7201 gctgctctcc cgcctggagc acttccacac ccgctatccg cacgtccgcc tgcgcatctc
     7261 caaccactcg acgccgcagg ccatccaggc gctcgaaaac ggtctggtcg acttcgcagt
     7321 cgtcacaagc ccggtggaaa tccgcaaacc gctgcacaag atcccgattt tcccattcca
     7381 ggaaattctg atcggcggat cgaaatatgc atccctttcc gcagagccgc atcatctcgg
     7441 cgaattgtca gaatatcctt ttatctcgct tgccaaagac accggcacgc gggagctgta
     7501 cgccgagtac tttctgaatc acagcctcgc cttccacccc gatatggagg cggcgacgac
     7561 ggatcagatt cttcctatgg tcgagtataa cctcggcatc ggattttatc cggaggagct
     7621 ggcacgcgat gccttaaaga gccgtaccgt ctgtcggatt ccgctcatcg aggaggcccc
     7681 caaacgtgaa atctgtctta tcatcaaccc gcggcagcac cagaacgcgg cggcaaaaga
     7741 gctgatcggg gagcttctgg aacgcgtata agattttatg aacttttgtt tgaaacagaa
     7801 aaagaccctg tctaccggcg gacggaacat cgctgctcca tctttcccgt atacagggtc
     7861 tcttttttat ttatgcctgc tgtctggaaa tctgctcttt tttatgcggc atggcaaaga
     7921 ccatcgccgt gtaggaaagc attgcgcctg tgatgtagaa gaacatcaga aatccgctcc
     7981 ggttcagcac agcgatgcgg acgattccaa tccagaaacc gacattggac gccagggaga
     8041 acacacgcgc aaacatcatg tttttctcct caccctttgc caggcggacg atcagcagca
     8101 gcatggccgc tgaaaagaat agtgtcatac agcactccag aaatacttcc attttttcag
     8161 ccttctttct tcactctctc ttatttctca gcggtacttt tccagtgaca tcagaaggaa
     8221 gctcaccaga agaagtccgc aggaaatcca gattacgtgg attccataca gcatggagaa
     8281 cacgcctccg attccggcgc cgatggcgaa agccagaatc acgccgaaat agtatccggc
     8341 ctgacggagc tgttcttttt tcttatcccg cagatacatg gacagcgctg cggttccgct
     8401 tcgcaggttt ccgatgcaca tggtgcttgc atacgaataa ccgccgacct tgcggaaggt
     8461 ctgtacctga atcgcgcacg caaacgatac aagcatcgcc gcgagcatat tataagagga
     8521 cggaataaat ccgacgatcg caaggatcac aatttccgcc gcgagaagta cctgccgcca
     8581 gtgcagcctc tttgcatacc ggtaatgctc ctggaaattt tctgccagaa atacacccat
     8641 cgcaaaaaag aggatcggga agagataccg aaggcctgcc gtaaagtttc cttccagaaa
     8701 gttctggctc atcagcacaa cgtttcccgt ctgggcattt gagaatactt catctctgca
     8761 attgtacgtg taggcatcct gaaaaccgcc ggacagcgcc agaaacatac tgttaataaa
     8821 tgcttccgac atctgatttt ccgagagcag gcgtcctaca aagcttgctt tcttttccat
     8881 acattttccc tttctttcaa cagacggccc ggctttttgg tgcctgccgt tttgcttccc
     8941 ttattataac cgcccgcttt ttgagatgga aaaacggata tcgtattatg ctcatatatt
     9001 tttcatatga ctatattgat ataaaattat tttaatatga tacaccgtaa aaaagggaga
     9061 aattttgtca ctttgcacaa acaaaatttc tccctttttg taaattgtct ttattgttct
     9121 aaaattttct tactttcttc ctctctgttt ctcactctgc tcgcggtgat actgcagcgt
     9181 gtagagctgg taatatcttc cctgcgctgc taaaagctcc tgatgcgttc catgctccac
     9241 gatttttccg tgggacagta cgataatctc atccgcatgc tggatcgtcg aaagacggtg
     9301 cgctacgatc agcatcgttc cgatgttctt catcttttcc atggaatcct ggatcagaag
     9361 ctcggtttcg gtgtcgatgt tcgccgtcgc ctcgtcaagg atcatgaccg aaggcttatg
     9421 gatgatggta cgcgcaaacg agagaagctg gcgctgtccg gcggagaagt tgtttcctct
     9481 ctcccgcacc ggctcgtcga ggccgtgatc cagcttgttg ataaactggt cagcattcac
     9541 ataatggcag acgtccatga tctcctcatc ggaaatcccc tcctcgcgga gaataatgtt
     9601 gctacggatc gttcccgaga acaggaagac atcctgcagc atctggccga attttttccg
     9661 cagggaatcg atcttgatct ttcggatgtc gatgccgtcg atcagaatct cacccttctg
     9721 gaactcgtag ttccggcaga tcagggaaag aatcgtcgat tttccggaac cggtggaacc
     9781 cacaaatgcg attgtctctt tcgggttgac gtggaacgaa acgcctttta acacccactc
     9841 gtccgggata taggaaaacc atacgtcgcg gaactcaatt tcgcccctga tgtcggtaag
     9901 ctcgatcgca tccggcgcat cctgcatttt gggctcgaga tccatgatcg aaaacacctt
     9961 ttccgcagat gccaaagccg actgcagcca gttgaactgc tccgccaggt tctggatcgg
    10021 gttgaagaat tttgaaatat acatgtaaaa gctgacgatc ataccgctgg taagcgtctg
    10081 gccgaggaac gacgttccct ccagatagcc cttgccgcct aaatacaaaa ggcagagcac
    10141 ggagctgata tacagcatgt agacgagcgg acggaaaata ccaaacacga aaatctgctc
    10201 ctgctgtgcg cggccgagtg cgttgctctt gtcggtaaac tcgcgcatct tggcatcctc
    10261 gcggttgaag atctgtgtga ttttcatacc ggaaaggttc tccgacagat acgtgttgat
    10321 gtccgtagta cagtctttta ctttccggtg tgctcttcgc gagaatttgc ggaaaataat
    10381 cgagaacagc acgataaacg gcacgaagca gagcaccatc agcgtcagct catagttcag
    10441 aaacagcatc gcaatcagaa taccgatgat cacgaagaag ttcttcagaa gattgaccag
    10501 aaggttggtg aacatcagcg aaatcgcgtt cgtatcgttt gtcgttctcg tgacaagctt
    10561 tccgaccgga atctcattta actgctcgtg ggaaaggcgc tcgatgtgga cgaacagatc
    10621 ctccctcata ttggaaatga ttttctgacc cgtcttctgc agaatcacgg cctggaaata
    10681 cgtgcacgcc atcgagacaa gaaggattgc cgcatagccc gctacccaca gaaacagggt
    10741 attcagcgcg aattttccaa cgacgagctc ctcaattttg ccgacgatca gcggcgaaat
    10801 gatatcgtac gcgatcgaaa acagcatcag aaaaccaaca acgataaact ggcggtaaaa
    10861 cggctttgcg taatccatca gacgctgcat gatttcactg tctttcatgt tccgctcaaa
    10921 gccggtttct ttttctccat ctttcactac aaacgcggcc acaatcagca gggtggaaat
    10981 cacgccgatg atcgcgccga cccagagaat cggataataa ttgtgcattt ctactcctcc
    11041 cccgcttcct cttcaagttt ctgcagatcg accatcttgc ggtacgccgt gcaggtccgg
    11101 tacagatcct cgtgttttcc gactgcatga acttctccgt cctcgacgaa cacgatcttg
    11161 tccatctgct ccaccgttga aatccggtgt gcgatcagaa ttgtcgtcag tccctcacgt
    11221 tttttgagat tgtcgagaat cgtgcgctct gttttggtat caactgcaga gacggaatcg
    11281 tcgagaatca gaatcggtgc gtgtttcatc agcgcacgcg caatcgagat tcgctgcttc
    11341 tgtccgccgg aaaccgtcac accgcgctcg ccaagaacgg tgcggtagcc ctctttgaac
    11401 tctgcgatat tgtcatggac atcggacatc atcgccgcat cctcaatggc ctcctggttt
    11461 tcgtcatcaa aggcgaagga aatattgccg gaaatcgtgt cggagaacag gaagttgtcc
    11521 tgcggcacgt aggcacagcc ctcacgcacg gagtggatgg aaacggtgtt gacatcgtgg
    11581 tcatcaatga acagcgttcc gtccggcacg ttgtaggtgc ggagcagcag atcgacgatg
    11641 gtcgtttttc cggaaccggt ctttccgacg atgccgacac tctcgcccgg ctcaatggta
    11701 aacgatacgt ttttcagcac atcgtactcc ccgtccggat agcggaaggt cagattgcgg
    11761 aactcgattt ttccatggat ggacggcaga tcctgcacac cgccgcggtc cttcacatcg
    11821 acttttgcgt caagcagttc cgtaatacgg tttaaggacg cttttccacg ggatgtcttc
    11881 tcgatcagca tggagaccgc catgatcggc catacaacgg ccgagaaata gccgatatac
    11941 tcgacaagct ggccggcgtc gaagcttccg cagtaaacga gatagccgcc gtagcctaag
    12001 attacgcaga tgacggactc gacgaacagc gttaccataa tgttcagcag tgtggaaatt
    12061 ttggtatatt ctacgttgat gtcctcgttc tcgcggttca gtctacggaa catcatcagc
    12121 tctttcgtct ctttgacgaa tgctttgatg acagcaagac cggaatagtt ttcctgcgcg
    12181 aaatcggaca gctcggaaaa ggccgcctgg cgctcttccc actttttcat caggtatttg
    12241 ccgagcgttg tgccggcggc catcagaaat gccatcggga tcatggaaag gccggtcagg
    12301 aagtggttca tgcgccacat tttgatcacg gcaagcaggc ctaaaaacag cgcatcgcag
    12361 aacatcagaa taccgtcgcc gaagcactcc tgcacggtat cgagatcgtt cgtgtaaaga
    12421 ctcatcaggt ttccgacttt gttgacctga taatactcct gggacagctc cttgctgtgc
    12481 gcgaacatgc aggtacgaag atcggtcacc acgcggatcg cggagccgaa aaagcagact
    12541 cgccacagga aacggccgac taccatgacg ataatggtaa taatcatggg ccggcagacc
    12601 tgatccagca gaaattccat atcaaacacc gccgcctggc cgttgaccag aacagggtcg
    12661 ccgttcatgc cgttgatgac catgcggtac agctccggta tgaaaagctg gaaataatct
    12721 acgagaatca gcgctgcaag accgagaatc agaatgggcg cattcttcag gtagtatttg
    12781 ttaatgtact ttccaaatat catatatttt ctccctcttt ttgtttttgc cattgtctgc
    12841 tatgatcttt ctgaccctaa cagtccttcc cttttccaaa gcgttttcgc cccggaatgc
    12901 ctcctttctt ctcctgcatt tcacagctcc gatgcaattt taacagagat aaacagaagg
    12961 aactggactt tgaaaaatcc aattccttct gttccaattc acattcctgc tccaaaagcg
    13021 ttccgcttct tctttgcagg acaccgttgt cctgtgaatg tttgttctat tttttagtat
    13081 acggttcttt ttctctcccg tcaagcattt cacgcgtttt tctgttttct tttttatttt
    13141 ttgccgtttt ccgctttttt tgcgcaaaat ctgcgcattc cggttaaaac agccaaaaga
    13201 agaagaaggc tgacgccaag tgtgatccac cacgttctgg tcacggctgc aatcagatat
    13261 cccaccaggc agacacacgc cgtaacggcc gcatactgca tctgcgtcga tacgtggttc
    13321 agatggttgc tctgcgcacc cgcacttgac ataaccgtcg tatccgagat cggcgaaata
    13381 tggtcaccgc agaccgcccc tgccaggacg gcggataccg agataatcat catctccaga
    13441 tttgttccgg cggcgaacat gcccgttgca atcggcacaa gaatcgcgaa cgttccccag
    13501 gaggttcccg tcgaaaagga aaggaacacc gccacggcga acagcaccac cgggataaaa
    13561 atacttgcgg atgcatggtc tcccacgacg cttccgacaa actgggccag gcccatcgca
    13621 tcaccgacgc cttttaaggt ccacgcaaag acgagaatcg taaccgccgg aaccatcagc
    13681 ttgaagccct cggacaggct gtccataaag ctcttgaagg tgatgacctt ccgcggcaga
    13741 tacagcgcca tcataaacag gatcgtcacg acggaggcaa aaatcagact cgtctctgca
    13801 tcacatcctg cgaacgcgga aatcacattg tccgcgccgc cgaggaagcc agtgtaaacc
    13861 atcgctccga tcgctgatac aatcagtacg gcaaccggga gcacgaggtc gatgactttt
    13921 ccgttcggat tgacctcctg ctcggaaacc tgctcaaact cctcgcctcc ggtcgtgaaa
    13981 agatcacctt tggcggcatt atcctcgtgg agcttcatct gaccaaaatc aaagccggtg
    14041 tagcagatgt aaaagaccat cgcaattgta aggatcgcat acaggttata cggaattgtc
    14101 cgcagaaaca gctggaatcc gctgattccc gcatccgccg gcacatacga gttgacggct
    14161 gccgcccagc ttgagatcgg cgcgataatg cagaccggcg ctgcggtgga gtcgatgatg
    14221 tatgcgagtt tcgcgcggga aaccttgtgg ctgtccgtga ccgggcgcat gacagagccg
    14281 actgtcagac agttgaaata gtcatccaca aaaatcagaa caccaagccc catcgttgcc
    14341 agaagcgcac tccgcttcgt tttgatcttc gtccccgccc attttccata cgccgccgag
    14401 ccgccggatt tcgccatcag cacgacaacc atgccaagca ggatatcgaa gatcagaatg
    14461 ttgatgttca tgctgttccg taaaatttca aagaagcttg tgaacgcatc ccacggatgg
    14521 aagccggtgt acaaaaggac accgactgcc gatccgatga acagggaact gtaaacttct
    14581 tttgttacaa atgcaagcag aattgcaagc agcggcggga cgagtgccca ccaggttccg
    14641 ataaacattg aaccattcat gtcgtctgtt ttccctttct gctgttaagt tcgtttacac
    14701 ttccggcgtc acaatttttc cagctactgc acatgctgcc gctgtctttg gagatgccag
    14761 ataaatttca actttagaag tacccattct tccaagaaag tttcggttgt tcgttgctac
    14821 aacacgctcg ccgtcagaga ggattccgcc cgctcttccg cagcacagac cgcagcctgg
    14881 atgtgtaatc atggcgcctg cctctgccag tgtatcgaga atgcccattt tgcttgcctg
    14941 cagataaatc ttgcggcttg ccggtgttac gatcagtttt acgtagtcgg ctactttttt
    15001 gccttttaaa acctcggctg ccgcctgcag atcctcgaga cggccgttcg tacaggaacc
    15061 gatgaagacc tgatcgatcg gggttccctc gagaacgctg gtatcacgga tcttgtctac
    15121 ctgggacggg catgccat
//
LOCUS       DAOMUF010000031        14774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_861_length_14774_cov_2.980433, whole genome shotgun sequence.
ACCESSION   DAOMUF010000031 DAOMUF010000000
VERSION     DAOMUF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 14774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14774
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_861_length_14774_cov_2.980433"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLN77_03325"
     CDS             complement(<1..497)
                     /locus_tag="PLN77_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRM43346.1"
                     /translation="MQPSSNNCYDTGLREECGVFGIYDLDGADVSPSIYYGLSALQHR
                     GQESCGIAVSDTSGPKGHVKSYKGLGLVNEVFKESKLEELSGNIGIGHVRYSTTGSTT
                     VENAQPLVLNYLKGSLSLSHNGNLVNAMELREQMENTGAIFHTSIDSEIIAYGIARER
                     VHSKTV"
     gene            962..2572
                     /locus_tag="PLN77_03330"
     CDS             962..2572
                     /locus_tag="PLN77_03330"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRM43347.1"
                     /translation="MAVKYVFVTGGVVSGLGKGITAASLGRLLKARGYKVTMQKFDPY
                     INVDPGTMNPIQHGEVFVTDDGTETDLDLGHYERFIDESLDKNSNVTTGKVYWSVLQK
                     ERHGDFGGGTVQVIPHITNEIKSRFYRGKEVDEERIAIIEVGGTVGDIESQPFLEAIR
                     QFQHDVGHENAILIHVTLIPYLKASGEMKTKPTQASVKELQGIGIQPDVIVCRSEHPL
                     TTEIKDKIALFCNVPSSHVLQNLDVEYLYEAPLAMEKENLAQVVCESLHLPCPEPDLS
                     DWTHMVEALRHPNKEVTIALVGKYIQLHDAYLSVVEALKHGGIASHANVHLKWVDSEL
                     VTEENVAEYLSDVDGVLVPGGFGNRGIEGMITAIRYARENKIPFLGLCLGMQLTIVEY
                     ARNVLGYHDAHSIEMNPNTMHPVIALMPDQDGIEDIGGTLRLGAYPCVLADGSKAQEL
                     YGEKEISERHRHRYEVNNDFRKALTENGMVLSGVSPDGRIVEMVELSDHPFYIATQAH
                     PEFKSRPNRPHPLFRGLIAAAAEYHAAK"
     gene            2802..7340
                     /gene="gltB"
                     /locus_tag="PLN77_03335"
     CDS             2802..7340
                     /gene="gltB"
                     /locus_tag="PLN77_03335"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRM43348.1"
                     /translation="MTQSKERVMGEEGLYRSSFEHDNCGIGAVVNIKGEKSHATVENA
                     LKIVENLEHRAGKDAEGKTGDGVGILLQISHKFFSKACSTLGFSLGGEREYGVGVFFF
                     PQNELKRNQAKKMFEIIVEKEGLELLGWRTVPTVPEVLGHKARECMPYIMQAFIKKPE
                     DVEKGIAFDRRLYVVRRVFEQSNDNTYVPSLSSRTIVYKGMFLVGQLRTFFRDLQDVD
                     YESAIAMVHSRFSTNTNPSWERAHPNRFIVHNGEINTIRGNADKMLAREETMSSPMLQ
                     DELHKVLPVVNTQGSDSAMLDNTLEFLTMSGMDLPLAVMITIPEPWANNDTISQEKRD
                     FYQYYATMMEPWDGPASILFSDGDVMGAVLDRNGLRPSRYYITNDGFLILSSEVGVLE
                     VPEEKIVLKERLHPGKMLLVNTVQGKVLNDEEVKEYYAKKQPYGEWLDSYLVQLKNIK
                     IPNLPVEEYTKEERMRLQKAFGYTYEEYRSSIGKMALNGVEATASMGIDTPLAVLSHE
                     HQPLFNYFKQLFAQVTNPPIDCIREEIVTSTSVYVGRDGNLLEEEPKNCQVLKINNPI
                     LTNTDMLKIKNMKVEGFKAVTVPITYYKSAPLDRAMDRLFVEVDRAHRAGANILILSD
                     RGVDENHVAIPSLLAVSAVHQHLVKTKKRTSLAIILESGEPREVHHFATLLGYGACAI
                     NPYLALDTIHELIEEGMIKKDYYAAVEDYNHAVISGIVKIAAKMGISTIQSYQGSQIF
                     EAIGISADVIDKYFTGTVSRVGGITLEDIAKDVDERHSQAFDPLELSTDLTLDSIGRH
                     KSRSQGEEHRYNPRTIHTLQESTRRGDYKMFKEYTAMVDSEESGYLRSLMDFDYPEQG
                     VPLEEVESVDSIVKRFKTGAMSYGSISQEAHETLAIAMNRLHGKSNTGEGGESDDRLE
                     SPERCSAIKQVASGRFGVTSKYLVSAKEIQIKMAQGAKPGEGGHLPAGKVYPWIAKTR
                     HSTPGVSLISPPPHHDIYSIEDLAQLIYDLKNSNVNARISVKLVSEAGVGTVAAGVAK
                     AGAQVILISGYDGGTGAAPRSSIHNAGLPWELGLAETHQTLIKNGLRNRVMIETDGKL
                     MSGRDVVIAALLGAEEFGFATAPLVTMGCVMMRVCNLDTCPMGIATQNPELRKRFRGK
                     PEYVINFMRFIAEEMREYMAKLGFRTVDEMVGRSDLLKRNDKVTNEHADRVDLSQILN
                     NPFAKAGEKVTFDPKDVYDFKLGATLDEKVLLKKFGPAMDKKEKKTVKVEITNTDRTV
                     GTILGAEITRKFGTSLEPDTYTVQCKGAGGQSFGAFIPKGLTLELEGDSNDYFGKGLS
                     GGKLVVYPPKARKYKPEENIIVGNVALYGATSGEAYINGIAGERFCVRNSGAYAVVEG
                     VGEHGCEYMTGGRVVVLGATGKNFAAGMSGGIAYVLDEKSNLYRNLNKEMISIELVES
                     KYDIAELKNMIEKHVAHTGSEKGKEILTHFDEYLPKFKKIIPNDYKRMMMLSAQMEDK
                     GLSSEQAQIEAFYESMRK"
     gene            7355..>8803
                     /locus_tag="PLN77_03340"
     CDS             7355..>8803
                     /locus_tag="PLN77_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRM43349.1"
                     /translation="MGKPTGFMEYQRKTGNTVAPLERIKNFKEFHLQLPKKEQQMQAA
                     RCMACGVPFCQAGTMIAGMASGCPLQNLVPEWNDLVYLGNYEQAYRRLTKTNCFPEFT
                     SRVCPALCEAACTCNVNGDPVCTKENERAIIENAWATGLITPQPPKVRTGKRVAVVGS
                     GPSGLAAAMQLNRRGHSVTVFERRDRVGGLLRYGIPNMKLDKSVIDRRVDLMKAEGIE
                     FVTNADIGKDVKADKLLKEFDRVLLCCGAANPRDIKAEGRDAKGIYFAVDFLTSVTKS
                     LLDSNFEDHAYIDAKGKHVVVIGGGDTGNDCVGTSIRLGAKSVTQLEMMPKAPDQRAE
                     NNPWPEWPKICKTDYGQEEAIAVFGHDPRIYQTTVTEFIKNKKGEVCQIKTVKLTPKK
                     DEKSGRMMMVPVEGTEEILPADLVLIAAGFLGSQKYVTDAFKVEVNQRTNVKTDDGKY
                     QTTKENVFTAGDMHRGQSLVVWAIREGREAAKA"
     assembly_gap    8804..8845
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8956..10287
                     /gene="glnA"
                     /locus_tag="PLN77_03345"
     CDS             8956..10287
                     /gene="glnA"
                     /locus_tag="PLN77_03345"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRM43350.1"
                     /translation="MAKYTKEVILQMAEEEDVEFIRLQFTDMFGMMKNMAITAGQLEK
                     ALNNQCVFDGSSFEGFVTIEEADMFLHPDLDTFAILPWRPQQGKVARLICDVYDREGN
                     LYAKSSRTILQKVIFEAKAMGYEFEISPECEFFLFHTDEDGGATSVTHDKGGYLDLGP
                     LDLGENARRDMVLTLEEMGIDVESSFHEVAAGQHELDFCPAAALRTADQLMTAKFAIR
                     TIAKRHGLHATFMPKPKTGVHGSGLHLSFSLFQEGKDVFADKSDEYGLSKEAYYFMGG
                     LMKHISGIAAITNPLVNSYKRLTPGYGAPIYVAWSAKNRSPLIRIPLTGDGIMAIELR
                     CPDSAANPYLALAVCMAAGLDGIKQKIVPPSPVDRNISDLTEEERNAMQITLLPGSLG
                     EAVAELKKDDFLCQVLGQEFVDGYINAKEEEWHEYLRQVSDWETEKYLYRI"
     gene            10311..10859
                     /locus_tag="PLN77_03350"
     CDS             10311..10859
                     /locus_tag="PLN77_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HRM43351.1"
                     /translation="MSSIVIVLPKIEDGKRIRDILAKRGYEIDAVCSTAAAALGEMNN
                     LNGGIVICGYKLPDMFFTDLNECMPSGFQMLLIASSRALSAVEGTGIMAVTMPLSVYE
                     LVNTLEMMLQSAARRRKKERAKPKVRSAEEQHIIDRAKSLLIERNHMTESEAHRYMQK
                     CSMDSGTNLVETAQMVLTLLTE"
     gene            10887..12101
                     /locus_tag="PLN77_03355"
     CDS             10887..12101
                     /locus_tag="PLN77_03355"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRM43352.1"
                     /translation="MFKINDNYLKLPGSYLFSTIAKKVAAYTEANPDAKIIRLGIGDV
                     TQPIAPAIIEKLHGAVDEMGHAETFHGYAPDLGYAFLRDTIAKNDYQGRGCDISSDEI
                     FVSDGAKCDCSNIQEIFSLDNKIAVCDPVYPVYVDSNVMAGRAGDYDAATERFTNVIY
                     MPCTAENNFTPELPKETPDLIYLCFPNNPTGAMVSKAELQKWVDYANKVGAIILYDAA
                     YEAYISEKDIPHSIYECEGARTCAIEMRSFSKNAGFTGMRLGFTVIPKDIKSGDVTLH
                     SLWARRHGTKYNGAPYIIQRAGEAVYSPEGKAQLKEQVAYYMKNAKVIYDGLKGAGYT
                     VSGGVNAPYIWLKTPGNMTSWEFFDHLLADANVVGTPGSGFGPSGEGYFRLTAFGTYE
                     NTVEAVERIKKL"
     gene            12268..12417
                     /locus_tag="PLN77_03360"
     CDS             12268..12417
                     /locus_tag="PLN77_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43353.1"
                     /translation="MKKAVKETPYAESDRKPMSPTESISMEETYNGEHSGAGLLNMRS
                     EQEIK"
     gene            12612..13940
                     /gene="aspS"
                     /locus_tag="PLN77_03365"
     CDS             12612..13940
                     /gene="aspS"
                     /locus_tag="PLN77_03365"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HRM43354.1"
                     /translation="MEFMTGVKKKEDISVKTLLETPEGTEVKLEGAVHIIRDMGEVAF
                     VILRKSEGLVQCVYEEGSVDFPLKDLKEESAVAVTGTVKKEDRAPGGVEVRLQTITVL
                     SEPTEPMPLAVNKWKMNTSLEAMLNMRPIALRNVRERAKFRIQEGVVRAFRDFLHSQG
                     FTEIHTPKIGAKGAEGGANIFKLEYFHRPAVLAQSPQFYKQMLVGVFDRVFEVGPVFR
                     AEKHNTKRHLNEYTSLDFEMGYIEDYTDIMAMETGFLQYMVELLKKDYAKELEILKVM
                     LPKTEEIPTVPFTKAKELVAEKYNRKIRNPFDLEPEEELLIGQYFKEEYDADFVFVTE
                     YPSKKRPFYAMDDPEDKRYTKSFDLLFHGLEITTGGQRIHDYKMLSEKIAARGMTEEG
                     MEQYLSAFKHGMPPHGGLGIGLERLTMKLIGEDNVRETTLFPRDLTRLEP"
     gene            13980..14273
                     /gene="gatC"
                     /locus_tag="PLN77_03370"
     CDS             13980..14273
                     /gene="gatC"
                     /locus_tag="PLN77_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRM43355.1"
                     /translation="MANVISDETMEYVGILAKLELKDEEKESARQDMQKMLDYVDKLN
                     ELDTSKVEPMTHIFEMGNVFREDEVTNGDDRDAMLANAPEVKEGQYKVPKTVE"
     gene            14289..>14774
                     /locus_tag="PLN77_03375"
     CDS             14289..>14774
                     /locus_tag="PLN77_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRM43356.1"
                     /translation="MEIKDMTAVQLSAAIKEGKITVADAVEASLAAVKASEESIHAFL
                     LVDEEGARARAAEVQKKIDAGELTGPLAGVPIAVKDNICTKGLATTCASKILQNFVPT
                     YDAEVITRLEEAGAVIIGKTNMDEFAMGSTTETSAYGVTKNPWNTEHVPGGSSGGSCA
                     AV"
BASE COUNT         4216 a         3509 c         3931 g         3076 t
ORIGIN      
        1 accgttttgg aatgaacgcg ttcccgcgca atgccgtagg cgatgatttc agagtcaatc
       61 gaagtgtgaa aaattgcgcc ggtgttttcc atctgctcgc gcagttccat agcgttgacc
      121 agatttccgt tgtgcgaaag ggaaagggag cctttcagat aattcagaac gagcggctgt
      181 gcattctcta cggtagtgga accggtggta gagtaccgga catggccgat gccgatattg
      241 ccggacagct cttctaattt ggattctttg aaaacttcgt tgacaaggcc aaggcctttg
      301 taggatttca catggccctt tggaccgctg gtgtcagaga cggcaattcc gcaggactcc
      361 tgtcctctgt gctgcagggc ggaaaggcca tagtagatcg aaggactgac gtcggcacca
      421 tccagatcgt aaatgccgaa aacaccacat tcctcccgga gaccggtatc ataacaattg
      481 tttgacgatg gctgcatagg tattctcctt ttttcagttg tagacctgca tgttttaaaa
      541 acattgatta tcatactcat ttttgtggat gatgtcaaga aaatcgtgat atgtataaaa
      601 tatggtgcag aaatttagga tttcgtggaa aatgtcgaaa ataataaaaa atagtaaaaa
      661 aaagttgcag gaaattcgag atgtgttata atcgtttttg attttgaatg aaagccccgg
      721 acagcagcta tggatgagcc atgcttgcat gagcgaattc atagatattg gccgggcaag
      781 acgacatgag taagtagggc gatagcccgg attacgaatg ttgtcagcag ttacgggagc
      841 gacgcaggcg cggagtaact ggctttcatc atagagatgt cgccggcagg cgacatttcc
      901 ttttagagaa acttgtgacg gaaaagaaaa tatcagaaga gaaaagcagg aggaaatcaa
      961 tatggcagta aaatatgtct ttgtgacagg cggagtagtc tccggccttg gaaagggaat
     1021 cacggcggcg tcccttggac ggcttttaaa agcccgagga tacaaagtga cgatgcagaa
     1081 atttgacccg tacatcaacg ttgacccggg tacgatgaac ccgattcagc acggcgaggt
     1141 tttcgttacc gatgacggca cagagacaga cctcgatctc ggccactacg agcgtttcat
     1201 cgacgagagc ctggataaaa attccaacgt gacgaccgga aaagtatact ggtctgttct
     1261 gcagaaagaa cgccacggtg atttcggcgg aggcaccgta caggtcattc cgcacattac
     1321 aaacgaaatc aagagccgtt tctaccgcgg aaaagaagta gacgaggagc gtattgccat
     1381 cattgaggtc ggcggaaccg tcggcgatat cgagagccag ccgtttttgg aggcaatccg
     1441 ccagttccag catgacgtcg gacacgagaa cgccattctg atccacgtta ccctgattcc
     1501 atatctgaaa gcttccggtg aaatgaaaac aaaaccgacg caggcgagcg taaaagagct
     1561 gcagggaatc ggtattcagc cggatgtcat cgtatgtcgg tccgagcatc cgctgaccac
     1621 cgagatcaaa gataaaattg ctctgttctg caacgttccg tcaagccatg ttctgcagaa
     1681 cctggacgtg gagtatctgt atgaagctcc gcttgccatg gagaaagaga acctggcgca
     1741 ggttgtctgc gagagccttc accttccatg cccggaaccg gatctgtccg actggaccca
     1801 tatggtagaa gcactccgcc atccgaacaa agaggtgacg attgcgctcg tcggaaagta
     1861 tatccagctg catgatgcat acctgagcgt tgttgaggca ttaaaacacg gcggaattgc
     1921 aagccatgca aacgtacacc tgaaatgggt ggattccgag ctggtaaccg aggagaacgt
     1981 ggcagagtat ctgagcgatg tggacggtgt tctggtaccg ggcggattcg gaaaccgcgg
     2041 tatcgaaggc atgatcacag cgattcgata tgcaagagag aacaagattc cgttcctcgg
     2101 cctctgcctc ggcatgcagc tgacgattgt tgaatatgca agaaacgttc tcggttacca
     2161 cgacgcacac agcatcgaga tgaacccgaa caccatgcac ccggtgatcg cactgatgcc
     2221 ggatcaggat ggaatcgagg atatcggcgg tacgttacgt ctcggcgcat atccatgcgt
     2281 cctggcagac ggatcgaaag cacaggagct gtacggtgag aaagagattt ccgagcgtca
     2341 ccgccaccgc tatgaagtaa acaacgattt ccggaaagca ctgaccgaaa acggcatggt
     2401 tctctccggt gtttctccgg acggaagaat cgttgagatg gtagaacttt cagatcatcc
     2461 gttctacatc gcaacgcagg cacatccgga gttcaaatcc cgtccgaacc gtccgcatcc
     2521 gctgttccgc ggcctgatcg cagcagcagc cgagtaccat gcggcaaaat aatcgataaa
     2581 tggtatgaaa aattaggtaa aatgcagtaa taacctaaga aagttaagaa aaaatataaa
     2641 aaattatata aaaattaaat ttgctcttta caggacgaaa aaaatagagt atagtaaggg
     2701 caatccagaa agaacggcgg ggaacaaaag attcccctcc attcttttca gaaaagacaa
     2761 aggataaccg aaaataagga aagtaaagga agggaagccg tatgacacag agcaaagagc
     2821 gtgtcatggg agaagaaggg ctgtaccgtt cttcctttga acacgacaac tgcggtatcg
     2881 gagccgtcgt gaatatcaaa ggtgaaaaat cccatgcgac cgtagaaaac gcattaaaaa
     2941 tcgtagaaaa tcttgaacat agagccggca aagacgccga gggtaaaaca ggagacggag
     3001 taggaatctt gttgcagata tcgcacaaat tcttttccaa agcctgttcg accctcggtt
     3061 tttcattagg cggagaacgg gaatacggcg tcggcgtatt cttcttccca caaaatgagc
     3121 tgaaacggaa ccaggcaaaa aagatgtttg agatcatcgt ggaaaaagag ggactggaac
     3181 tccttggatg gagaaccgtg ccgaccgttc cggaagttct cggccataag gcaagagagt
     3241 gtatgcctta catcatgcag gcattcatca aaaaaccgga ggatgtggaa aagggaatcg
     3301 ctttcgaccg tcgtctgtac gtcgtacgcc gtgttttcga acagagcaac gacaacacct
     3361 acgtgccatc cctttccagc cgtaccatcg tatacaaagg tatgttcctc gtaggacagc
     3421 tccgcacctt cttccgcgat ctgcaggatg tcgattatga atctgcaatc gccatggtgc
     3481 attcccggtt cagcacgaac acgaacccaa gctgggagag agcccatccg aaccgtttca
     3541 tcgtacacaa cggcgagatc aacaccatcc gcggaaacgc cgacaagatg ctggcccgtg
     3601 aggagacgat gtcctccccg atgctgcagg atgaactgca caaggttctg ccggttgtca
     3661 acacccaggg ttctgactct gcaatgctcg acaacacact cgagttcctg accatgagcg
     3721 gtatggatct tccgctggct gtcatgatca cgatcccgga gccgtgggca aacaacgata
     3781 cgatttccca ggagaaacgt gatttctatc agtattatgc aacgatgatg gagccgtggg
     3841 acggaccggc atcgatcctg ttctccgacg gagatgtgat gggagcagtg ctcgaccgca
     3901 acggtcttcg tccttcccgt tactacatca ctaacgatgg atttctgatc ctctcctctg
     3961 aggtcggtgt tctggaagtt ccggaagaaa aaatcgtctt aaaagagcgt ctccatccgg
     4021 gcaagatgct tctcgtcaac accgtacagg gaaaagtctt aaacgatgag gaagtcaaag
     4081 aatattacgc aaagaaacag ccgtacggcg aatggctcga cagttatctg gtacagttaa
     4141 agaatatcaa aatcccgaat ctgccggtcg aagagtacac gaaagaagag cggatgcgcc
     4201 tgcagaaagc gttcggctac acctacgagg agtaccgttc ctccatcgga aagatggcat
     4261 taaacggtgt cgaggcaacc gcttcgatgg gtatcgatac cccgcttgcc gttctctccc
     4321 atgagcatca gccgctgttc aactatttca aacagctctt cgcacaggta acgaacccgc
     4381 cgatcgactg catccgtgag gagatcgtaa cttccacaag cgtttacgtc ggacgtgacg
     4441 gaaacctgct tgaggaggag ccgaagaact gccaggtatt aaagatcaac aatccgatcc
     4501 tgaccaatac cgatatgctg aagatcaaga acatgaaagt cgaaggcttc aaggcagtga
     4561 ccgttccgat cacctactac aagagcgcac cgcttgaccg tgcgatggac cgccttttcg
     4621 tcgaggttga ccgtgcacac cgggcaggag ccaacatcct gattctctct gaccgcggcg
     4681 tcgatgagaa ccatgtggcg attccatccc tcctggctgt atctgcggtt catcagcatc
     4741 tggtaaaaac gaaaaagaga acttctcttg cgattattct ggagtccggc gagccgcgcg
     4801 aggtgcacca tttcgcaaca ctgctcggct acggtgcctg cgcaatcaac ccgtatctgg
     4861 cactcgacac gatccatgag ctgatcgaag agggcatgat caagaaagat tactacgcag
     4921 ccgttgaaga ttacaaccac gcggtcatct ctggtatcgt aaaaatcgca gcgaagatgg
     4981 gtatctccac gatccagtcg tatcagggtt cccagatctt cgaggccatc ggtatctcag
     5041 cagatgtcat cgacaaatac ttcaccggta cggtcagccg tgtcggcggt attaccctgg
     5101 aagacattgc aaaagatgtc gatgagcgtc attcccaggc attcgatccg ctggagctgt
     5161 ctaccgatct gacactggac agtatcggcc gccataaatc gagaagccag ggcgaggagc
     5221 accgctacaa tccgcgcacc attcatacac tgcaggagtc cacaagacgc ggcgactaca
     5281 agatgttcaa agagtacaca gcaatggtcg acagcgagga gagcggctat ctgcggagcc
     5341 tgatggactt cgattatccg gagcagggcg ttccgctcga agaagtcgag agcgtagatt
     5401 ctatcgtaaa acgattcaaa acaggtgcga tgtcctacgg ttctatttcc caggaggcac
     5461 atgagacact ggcaattgcc atgaaccgtc tgcatggaaa atccaacact ggtgagggcg
     5521 gtgagagtga tgaccgtctc gaatccccgg agagatgctc cgcgatcaaa caggtagcat
     5581 cgggaagatt cggcgttacc agcaaatacc ttgtcagtgc aaaagagatc cagatcaaga
     5641 tggcccaggg tgcaaaaccg ggcgagggcg gccacttacc ggccggaaag gtttacccgt
     5701 ggattgcaaa aacacgtcac tccacaccgg gtgtatccct gatttccccg ccgccgcacc
     5761 acgacatcta ctccatcgag gaccttgcac agctgattta tgacctgaaa aactcgaatg
     5821 tgaatgcaag aatctccgtt aaactggtat ccgaagcagg tgttggtaca gtcgcagccg
     5881 gtgttgcaaa agccggagca caggttatcc tgatttccgg atatgatgga ggaacgggag
     5941 cagcaccgag aagttccatc cacaatgcag gacttccatg ggagttaggc cttgccgaga
     6001 cccatcagac gctgatcaaa aacggcctcc gcaaccgtgt catgatcgag accgatggta
     6061 agctgatgag cggccgtgac gttgtgattg cagcactcct tggcgccgag gaattcggtt
     6121 ttgcgaccgc accgcttgta acgatgggat gcgttatgat gcgtgtctgc aacctcgata
     6181 cctgcccgat gggtattgca acccagaacc cggagctgcg caaacgtttc cgcggcaaac
     6241 cggagtacgt catcaacttc atgcgcttca tcgcagaaga gatgcgggaa tacatggcaa
     6301 aacttggctt ccgcaccgtt gacgagatgg tcggccgttc cgacctctta aagagaaatg
     6361 ataaagtgac gaacgagcac gcagaccgcg tagacttaag ccagatcctg aacaatccgt
     6421 ttgcaaaagc aggcgagaag gtaacgttcg atccgaaaga tgtttacgac ttcaaacttg
     6481 gcgcaaccct ggatgagaag gtactcttaa agaaattcgg tccggcaatg gataagaaag
     6541 agaagaagac cgtcaaagtc gagatcacca acaccgatcg taccgtggga acgattcttg
     6601 gtgccgagat caccagaaaa ttcggcacct ctctggagcc ggatacgtac accgtacagt
     6661 gcaagggtgc cggcggacag agcttcggag catttattcc aaaaggactg accctggaac
     6721 ttgagggcga ttcgaacgat tacttcggaa aaggcctctc tggcggaaaa ctggttgtct
     6781 acccgccgaa agcaagaaag tataaaccgg aagaaaacat catcgtcgga aacgttgccc
     6841 tctacggcgc aacgagcggt gaggcgtaca tcaacggtat cgcaggagag cgtttctgtg
     6901 tcagaaactc cggtgcttac gccgttgtcg agggtgtcgg tgagcatgga tgcgagtata
     6961 tgaccggcgg ccgtgtcgtt gtcctcggcg caaccggaaa gaactttgca gccggcatga
     7021 gcggcggtat cgcttatgtc ctggatgaaa agagcaacct gtaccgcaat ttgaacaagg
     7081 aaatgatttc gattgaactg gtagaaagca aatacgatat tgcagagctg aaaaacatga
     7141 ttgaaaaaca cgtcgcacac accggctctg aaaaaggtaa agaaattctc acacactttg
     7201 acgaatatct gccgaaattc aagaagatca ttccgaatga ctataagcgc atgatgatgc
     7261 tgagtgccca gatggaagac aagggtctta gcagcgaaca ggcacagatt gaagcatttt
     7321 acgaaagcat gcgtaagtag gaggaagaag aattatgggt aagccaactg gatttatgga
     7381 atatcagcgc aagacgggaa acaccgttgc cccgcttgag cgtatcaaaa actttaagga
     7441 atttcatctg cagctcccga aaaaggagca gcagatgcag gctgcccgct gtatggcatg
     7501 cggtgtaccg ttctgccagg cgggaaccat gattgcggga atggcctccg gctgtccgct
     7561 gcagaacctc gttccggagt ggaatgacct tgtgtatctt ggaaactatg agcaggcata
     7621 ccgccgcctc accaagacaa actgttttcc ggaatttacc tcccgcgtct gcccggcact
     7681 ctgcgaagca gcctgtacat gcaacgtaaa cggagacccg gtctgcacga aggaaaatga
     7741 acgcgcgatc atcgaaaacg catgggcaac cggcctgatt acgccgcagc cgccgaaagt
     7801 gcgcaccgga aaacgcgttg ccgttgtagg aagcggtcct tccggccttg cagcagcgat
     7861 gcagctcaac cgccgcggcc attctgttac ggtctttgaa cgccgcgacc gcgtgggcgg
     7921 acttttacgc tacggtattc cgaacatgaa actcgataag agcgtcatcg accgccgtgt
     7981 ggatctgatg aaggccgagg gcatcgagtt tgtgacgaat gctgatatcg gaaaagatgt
     8041 aaaagctgac aaactcttaa aagaatttga ccgtgtcctg ctctgctgcg gtgcggcaaa
     8101 tccaagagat atcaaggcag agggaagaga tgcaaagggc atctactttg ctgtcgattt
     8161 cctgacttcc gtaacgaaga gtcttctgga ttccaatttt gaggatcatg cctacatcga
     8221 tgccaagggc aaacacgttg ttgtcatcgg aggcggcgac accggtaatg actgtgtcgg
     8281 aacttccatc cgtctcggtg ccaaatctgt gacacagctt gagatgatgc cgaaagcacc
     8341 ggatcagcgt gccgagaaca atccgtggcc ggagtggccg aagatctgca agacggatta
     8401 cggccaggaa gaggcaatcg ccgtattcgg ccatgatccg cggatttacc agacaaccgt
     8461 aacggaattt atcaaaaaca aaaaaggtga agtctgccag atcaagaccg taaaactgac
     8521 accgaaaaaa gatgagaaat ccggccgcat gatgatggtt ccggttgagg gaaccgaaga
     8581 gattctcccg gcagatctcg ttcttattgc agcaggcttt ttaggatccc agaaatatgt
     8641 gaccgatgca tttaaagtgg aagtcaacca gcgcaccaac gtgaagacag atgacggaaa
     8701 ataccagaca acaaaagaaa atgtcttcac cgcaggcgat atgcaccgcg gccagtccct
     8761 ggtcgtatgg gcgatccgtg agggacgcga ggcagcaaag gcannnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnngctat aatatgccca aaacaagggt gtttttacgc
     8881 aaggtgtctt tctgccctgc cgcgtgaaag cacccttttc gtttagaatc gtctttgata
     8941 aaggagagaa tcgatatggc aaagtatacg aaggaagtga tccttcagat ggcggaggaa
     9001 gaagatgttg aatttatccg cctgcagttt accgatatgt ttggaatgat gaaaaatatg
     9061 gccatcaccg ccggacagct ggaaaaagcc ctgaataatc agtgcgtttt cgatggctct
     9121 tcgttcgaag gatttgtcac catcgaggaa gcagacatgt ttctgcaccc ggatctcgac
     9181 acctttgcaa tacttccgtg gcgtccgcag caaggaaagg ttgcaagatt gatctgtgat
     9241 gtgtatgata gagaggggaa tctttatgca aagagttccc gaaccatcct tcagaaggta
     9301 attttcgaag cgaaagcgat gggctatgag tttgaaatct caccggagtg tgaatttttc
     9361 ctgttccaca ccgatgagga cggcggagca acgagtgtaa cgcacgacaa aggcggttat
     9421 ctggatctgg gaccgctgga cctgggagaa aacgcgcggc gcgatatggt gctgaccctc
     9481 gaagaaatgg gaatcgacgt tgagtcttcc ttccacgagg tagcggcagg ccagcacgaa
     9541 ctggatttct gtccggctgc agcacttcgg acggcagatc agctgatgac tgccaaattt
     9601 gctatccgta cgattgcaaa acgtcatggc cttcacgcaa cctttatgcc aaaaccaaaa
     9661 accggagttc acggatcggg tcttcatctt tccttctccc tgtttcagga gggaaaagat
     9721 gtgtttgccg acaaatccga tgaatacggg ctcagcaaag aggcatatta ctttatgggc
     9781 ggactgatga aacacatcag cggaatcgca gcgattacca acccgcttgt aaattcctac
     9841 aaacgtctga caccgggata cggtgcaccg atctacgtgg catggtctgc aaagaacaga
     9901 agcccgctga tccgcattcc gctgacggga gacggtatca tggccattga acttcgatgc
     9961 ccggattcgg cagccaatcc gtacctggca cttgctgtct gcatggcagc aggacttgac
    10021 ggtatcaaac agaaaatcgt tccgccgtcc ccggtggacc gcaatatttc cgatctgaca
    10081 gaagaagaac gaaacgcgat gcagatcacc cttctcccgg gaagccttgg agaagcggtc
    10141 gcagagttaa agaaagacga ttttctgtgt caggtactcg gccaggaatt tgtggatgga
    10201 tatatcaatg ccaaagaaga agagtggcat gagtatctcc gccaggtatc cgactgggag
    10261 accgagaaat atctgtaccg tatttagacg acgaaggggc ctgaaaatgc atgagcagca
    10321 tagtgatagt acttcccaaa atagaagatg gaaagcgcat ccgggatatt ctagcaaaaa
    10381 gaggatatga aatcgatgct gtctgttcga cagcggcagc ggcgctcgga gagatgaaca
    10441 acttaaacgg cggtatcgtc atctgcggct acaagctccc ggatatgttt tttacggatc
    10501 tgaatgagtg tatgccgtcc ggcttccaga tgcttctgat tgcatcgagc cgcgcattaa
    10561 gtgcggtgga gggaaccgga atcatggcgg ttacgatgcc gctttccgtg tacgagctgg
    10621 tcaatacact ggagatgatg ctgcagagtg cggcacggcg cagaaagaaa gagcgggcaa
    10681 agcccaaggt gcgcagcgcc gaggaacagc acatcatcga tcgtgcaaaa agccttctga
    10741 tcgaacgaaa tcacatgacc gagtcggagg cccatcgcta catgcagaaa tgcagcatgg
    10801 acagcggcac caatctggtt gaaacggcac agatggtgct gacgcttttg acagagtaag
    10861 aaaaagtgaa aaggagcgac ataataatgt tcaaaatcaa tgacaattat ctgaaattgc
    10921 cgggaagcta tctgttttcc accattgcaa aaaaagttgc agcttacacc gaggcaaatc
    10981 cggacgcgaa aatcatccgt cttggaatcg gcgacgtaac ccagccgatc gcaccggcga
    11041 tcattgaaaa gctgcacgga gcagtggatg agatgggaca cgcagagacc ttccacggat
    11101 atgcaccaga tcttggatat gctttccttc gcgataccat cgcaaaaaat gattatcagg
    11161 gcagaggatg cgatatctca tctgatgaaa tttttgtatc ggacggcgca aaatgcgact
    11221 gcagcaacat tcaggagatc ttcagcctgg acaacaagat cgctgtatgc gacccggttt
    11281 atccggttta tgtagactcc aacgtaatgg caggccgtgc cggtgactac gacgcagcaa
    11341 ccgagcgttt cacgaatgtg atttacatgc cgtgtaccgc agaaaacaac ttcaccccgg
    11401 agctcccgaa agaaaccccg gatctgattt atctgtgctt cccgaacaac ccgaccggcg
    11461 ctatggtttc caaagccgag ctgcagaaat gggttgatta tgcaaataaa gtaggagcca
    11521 tcatcctgta tgatgctgca tacgaggcat atatctcaga aaaagacatt ccgcattcca
    11581 tctatgagtg cgagggtgca agaacatgtg ccatcgaaat gcgcagcttc tccaagaacg
    11641 caggctttac cggtatgcgt cttggattca ccgttatccc gaaagacatc aaatccggcg
    11701 atgtgactct gcacagcctg tgggcacgcc gtcacggaac gaaatacaac ggagctccgt
    11761 acatcatcca gagagccggc gaggctgttt actctccgga aggaaaagca cagttaaaag
    11821 agcaggttgc ttactatatg aagaatgcaa aagtcatcta cgacggatta aagggcgcag
    11881 gctacaccgt atccggcggt gtcaacgctc cgtatatctg gctgaaaaca ccgggaaata
    11941 tgacttcctg ggagttcttt gatcacctgc ttgcagacgc aaacgtagtc ggaacaccag
    12001 gatcaggctt tggcccgagc ggagaaggct acttccgtct gactgcattc ggtacgtatg
    12061 agaatacagt agaggcagtg gagagaatca aaaaactgta aattgtgtgc gaaataccaa
    12121 taaaacggaa tttctgcgca attttataaa cacgcgaaaa aataattttg tatagataaa
    12181 attcacgaaa aatgcattag aatttatata aaatccgtaa aaattaggtg tttacaaatg
    12241 caaaactgct gtgctataat gcacagtatg aaaaaggcag tgaaggaaac tccgtatgcg
    12301 gaatctgaca ggaagccgat gtcaccgact gagagcatca gcatggagga aacatacaat
    12361 ggggaacatt ccggagccgg tctgctgaac atgagatccg aacaggaaat caagtaggca
    12421 gatccgtgat acgcgttaag tataaatgag tgggcgtttt cccccggaaa accggagaga
    12481 aaaacgcaat gcgggtggta ccgcgggaac ataaaatatc ccgtcccgga acagtagtta
    12541 aaaaactgtt ccgggacttt tttatttgaa aaagcaatcc gggacaggcg agaaagagag
    12601 gagaacaaga catggaattt atgacaggcg taaagaaaaa agaagacatc tccgtaaaaa
    12661 ctctgctgga gacaccggaa ggcacagaag taaaactcga aggagccgta cacatcatcc
    12721 gtgacatggg cgaggttgcc tttgtcatct taagaaagag cgagggactc gttcagtgtg
    12781 tgtacgaaga aggaagcgtt gacttcccgc tgaaagatct gaaagaggaa tcggcagttg
    12841 ccgtaaccgg aacggtcaaa aaagaggacc gcgcaccggg cggcgtcgaa gtcaggctgc
    12901 agaccatcac cgttctttcc gagccgaccg agccaatgcc gctcgcagtc aacaaatgga
    12961 aaatgaacac ctcccttgaa gcaatgctga acatgcggcc gatcgctctt cgaaacgtca
    13021 gagagcgtgc aaaattccgc atccaggaag gcgtggtacg ggcgttccgt gatttccttc
    13081 attcccaggg attcaccgaa atccatacac cgaagatcgg cgcgaaaggc gctgagggcg
    13141 gcgcaaacat cttcaagctg gaatatttcc accgcccggc tgtcctggca cagagcccgc
    13201 agttctacaa acagatgctc gtcggcgtgt ttgaccgtgt ctttgaagtc ggaccggttt
    13261 tccgtgcaga gaaacataac acgaaacgtc atctgaacga atataccagc cttgactttg
    13321 agatgggcta catcgaggat tacaccgaca tcatggcaat ggaaaccgga tttttacagt
    13381 acatggtgga gcttcttaaa aaggattacg caaaagagct ggagatcctg aaagttatgc
    13441 ttccaaagac agaagaaatc ccaactgtac cgttcacgaa agcgaaagag ctggtagccg
    13501 agaaatacaa ccgaaagatc cgcaacccgt tcgaccttga gccggaggag gagcttctga
    13561 tcggccagta tttcaaagag gagtacgacg cagacttcgt attcgtcacc gaatatccgt
    13621 cgaaaaagag accgttctat gcgatggacg acccggagga taagagatac acaaagagct
    13681 tcgacctgct gttccacggt ctggagatta caaccggagg ccagcgtatc cacgattaca
    13741 agatgctctc tgaaaagatc gcagcccgcg gcatgacaga agagggcatg gagcagtact
    13801 tgagtgcttt caaacacggt atgccgccgc acggaggtct tggcatcggt ctggagcgtc
    13861 tgaccatgaa gctgatcggt gaggacaacg tccgcgagac aacgctgttc ccgcgtgatc
    13921 ttacaagact ggagccgtaa gcggatcctg ctggaaaata aaaaaggaga atgagtgaaa
    13981 tggcaaatgt gatttcagat gaaacgatgg aatatgtcgg catcctggca aagctggaat
    14041 taaaggatga ggaaaaagaa tccgcacggc aggatatgca gaaaatgctg gattatgtag
    14101 ataaattaaa cgagctcgac accagcaagg tcgagccgat gacccacatt ttcgagatgg
    14161 gaaatgtatt ccgcgaggat gaagtaacca atggcgacga ccgcgatgca atgcttgcca
    14221 atgcaccgga agtcaaagag ggccagtaca aggttccgaa aacggtagaa tagcaggagg
    14281 agattgatat ggaaatcaaa gatatgacag cagtacagct ttctgccgca atcaaagaag
    14341 gaaagatcac tgtagcggac gctgtagaag cgtctcttgc ggcagtcaaa gcatcggaag
    14401 aatcaattca cgcattcctt cttgtggatg aagagggtgc aagagcacgc gcagcagaag
    14461 tgcagaagaa aattgacgca ggagagctca ccggtccgct ggccggcgtt ccgattgcgg
    14521 tcaaggacaa catctgtacg aaaggtcttg cgacgacgtg tgcgtcaaag atcctgcaga
    14581 actttgttcc gacgtacgat gcagaggtta tcacacggct cgaagaggcg ggagccgtta
    14641 tcatcggaaa gacaaacatg gatgagtttg cgatgggaag tacgacagag acttccgcgt
    14701 acggcgtgac gaaaaatcca tggaacaccg aacacgttcc aggcggttcc tccggcggtt
    14761 cctgtgcagc cgtt
//
LOCUS       DAOMUF010000032        14696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_868_length_14696_cov_3.793252, whole genome shotgun sequence.
ACCESSION   DAOMUF010000032 DAOMUF010000000
VERSION     DAOMUF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 14696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14696
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_868_length_14696_cov_3.793252"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1984)
                     /locus_tag="PLN77_03380"
     CDS             complement(<1..1984)
                     /locus_tag="PLN77_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRM43357.1"
                     /translation="MQPKFKKMLYGGDYNPNQWPKEIWDEDMKLFHQAGINSTTINVF
                     SWAKIQPSENEYDFTELDEIVDTLTKEDVQIVMATSTGALPAWMVHRYPEVARTDFEG
                     RHHKFGHRHNACPNSPVFQKYAKRLAEKLAERYGDNKNIVCWHISNEYGGECYCENCA
                     KAFRVWLKDKYKTLDEVNKAWNMEFWGHTIYDWDEIVPPNDLGDGMPWGSGTAFAGMS
                     LDYMRFNSDGMLNNYKMERDAIRKYDKETPITTNLMGTYKGLDYFKWAKEMDIVSWDN
                     YPSYNTPWSHVAMTHDLMRGLKDAPFMLMEQTPSQQNWQPYNSLKKPGQMRAQSYQTV
                     AHGADTIQFFQLRRSVGGCEKFHGAVIAHAGTADTRVFREVTQLGKELEKIGSRVMGS
                     ENHAKVGIIFDWENYWALEYTSGPNRDLRYVDQIEQMYNYFYNKNIAVDMIPIDADFS
                     KYDLVAAPVLYMVKDGVAESLEAYVQAGGTLITTYMSGIVGQSDNVHLGGYPGPLRKM
                     AGVWVEEIDALAPEQRNKIRFADGTEEECRLVCDLMHLEGAEEIAQYESDFYAGMTAV
                     AKNQFGKGTVYYVGTDLSESGLAKVLAMAADGAAIQPVIGEATALEITKRQADGEDLY
                     FIINFKDEEIPLPAVFDGKEDILTGEKVQGGEMLKKY"
     gene            2146..2967
                     /locus_tag="PLN77_03385"
     CDS             2146..2967
                     /locus_tag="PLN77_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM43358.1"
                     /translation="MPIFFKPSASDFSLSADSIGNRWLQEPVVRPNGFPLYHWIQTER
                     SEGLLTLEGQELLLKPGDGVLIAPHVPHEYRENDGSGMWFTSFLTFDGKLSDDLYKIC
                     AVSPYLFVPASEGAWFQEWIDRIIDSHFLKNDIDDAALSVGCFEFLTRLSQLRTGLSR
                     TEHPLYLQYVRPAMQEIESHLSDPLNVDDLAEQLHITPQYLTRLFQRFVGSSVRSYIS
                     VLRISRAKELLISRPYTTLDGIAALCGYRDVSYFISVFRQQTGTTPKQFRKLYGI"
     gene            complement(3047..4441)
                     /locus_tag="PLN77_03390"
     CDS             complement(3047..4441)
                     /locus_tag="PLN77_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM43359.1"
                     /translation="MKYDVIIIGAGPSGIFCAYELLKEKPDLKVLMIEKGRSIESRQC
                     PKRKTKKCVNCKPCSITTGFAGAGAFSDGKLSLSPDVGGNLPEILGYEKTVELIHESD
                     QIYLDFGADKNVYGVDKEKEIREIRRRAINANLKLIECPIRHLGTEEGYKIYSRLEDH
                     LKEIGVEMKFNTMVNDITIEDGRATAVTLEDGTVYEADEIVSAVGREGADWFSHICNG
                     HGIETEVGTVDIGVRVEVRDEVMEFLNKNLYEAKLVYYTPTFDDKVRTFCTNPSGEVA
                     TEYYENGLAVVNGHAYKSQEFKTNNTNFALLVSKNFTKPFKTPIEYGKQIAQLSNMLC
                     DGKILVQTYGDFKRGRRTTEERLCRNNLIPTLKDAVPGDLSLVFPHRIMVDIAEMIEA
                     LDKVTPGIASDETLLYGVEVKFYSNKVVVDKDFCTSVKGLRAIGDGASVTRGLQQASA
                     NGLSVARSILKSMK"
     gene            complement(4466..4729)
                     /locus_tag="PLN77_03395"
     CDS             complement(4466..4729)
                     /locus_tag="PLN77_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HRM43360.1"
                     /translation="MGEYTEECINTFLMNQGQLFDEPVAENYDEAEAFLEDCLAVVAD
                     NLDDVRAYMEEEGMDVEEMSDAELEEQSEVFALPDGQYLIVMG"
     gene            complement(4753..5421)
                     /locus_tag="PLN77_03400"
     CDS             complement(4753..5421)
                     /locus_tag="PLN77_03400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM43361.1"
                     /translation="MIKACIFDLDGTIADTVESIAHAVNRVLEHFGLVPRPVEAFNFY
                     AGDGFDLAVERALKDAGDAELNYLQEGIRLGRAWFNEDPLYHVKPYPNMRETLTALRE
                     EVPIAVCSNKPHEAAIHVVESVYGPGFFNRIQGQTAEIPRKPSPIGALAIADALGARP
                     EECLYFGDTNTDMQTGNAAGMFTVGVTWGFRPRQELIDNHAMELLDRPEEILTLFHRK
                     NQEK"
     gene            5596..6417
                     /locus_tag="PLN77_03405"
     CDS             5596..6417
                     /locus_tag="PLN77_03405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM43362.1"
                     /translation="MNQTVKIFFTDMDDTLLNSEKQVTKENMDAISEALAAGHKIVFN
                     TGRPLSGFLPILKELGLDREGCYAIAFNGGLIYDCAAQKTLHRRTIPLEYARVIFEKT
                     QEAGLYCHTYSDTSLLCRHAGEETRVYTQAIHIPVVEDPLLPEHLAEEPCKMIVIDLH
                     DRAKMDAYRAAMEPWAKGKISMFYSSPHYLEHVPEHVSKGHAVKKLCELLQIPLANTV
                     AAGDQENDISMIEAAAVGAAMKNATDAVKASADYITENDCNHSGVAEILRKFILA"
     gene            6561..6932
                     /locus_tag="PLN77_03410"
     CDS             6561..6932
                     /locus_tag="PLN77_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43363.1"
                     /translation="MSRKSKRILLTLASAAVGAACGAAVYFIRKGKADLETDFDDFKD
                     EFEEEEPAPAERTYTTLPKTKGAEDAAPDPESAACNEGSETADAEAESETAESEEEDA
                     EKESCADREVSPQEESEVTAE"
     gene            7015..8817
                     /gene="pepF"
                     /locus_tag="PLN77_03415"
     CDS             7015..8817
                     /gene="pepF"
                     /locus_tag="PLN77_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HRM43364.1"
                     /translation="MELAKRDDVPVELTWDLSLIYPTEEAMLADAQKMKELSLSMEAS
                     YKGNLTDAATINHCLDDYQEVYRLITLTANYCDLAVSVDYYNSANQTRNDRINSLISE
                     IFSRLTFIESELSEQSEDVLNEAMQQSDTNRCYLAEILRNKEHRLNPETERAISALSQ
                     TFSAPYQIYNMAKLADMKFDSFTVNGKEYPLGYSLFEDNYEYEKDTDIRRSAFSAFSA
                     KIRQYENVTAAAYNAQLQTEKTMATLRGFDSVIDSLLFPQQVSRELYNRQIDLITTRL
                     APHMRKYARLLKKVHNLDRMTFADLKIAVDPEYDPSVTIEESKQYIEKGLAILGDDYV
                     SMIQEAYKKRWVDFAQNQGKSTGGFCASPYGKGSFILLSWNNRMADVFTLAHELGHAG
                     HFRLCNGAQAILDTEVSSYFVEAPSTMNELLMAHYLLKTTPDKRFRRWVLSCMISNTY
                     YHNFVTHLMEAAYQREVYKLIDAGDSVQAETLSSIMKETLQKFWGDDVEISDDAALTW
                     MRQPHYYMGLYSYTYSAGLTVATQVCKRIETEGQTAVDDWKKVLTAGSTKTPEELAKM
                     AGVDISTDAPLLDTIETIGSIIDEICELTEELGC"
     gene            complement(8915..10321)
                     /locus_tag="PLN77_03420"
     CDS             complement(8915..10321)
                     /locus_tag="PLN77_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM43365.1"
                     /translation="MNELTISLDPHRKTPLYEQIYGFIREEIRGGRIRAGERLPSARA
                     LSGYLSVSRSTVDLAYEQLVSEGYLESVPCKGYFVCEIEGLYRLDEKKEETKEDDAAE
                     QMPFRYDFAVTGTAPGGFPQNSWKKISKEVLLDADDSLFQLGDAKGEHGLREAIRDYL
                     HHARGVNCRTEQIIVGAGNDYLLMLLSVILGRGHKVAMENPTYISAYRCFAKLGYAMC
                     TISMDEAGMRPAELEKSGADLAYIMPSHQFPMGMVMPMKRRMQLLAWASRENGRYLIE
                     DDYDSEFRYKGRPIPALQGNDTEGKVIYLGTFSRAIAPSIRISYMVLPKTLLPAYEEN
                     GRCFSATVSKTDQKILEEFMRSGAFERHLNRMRAQYKGKHDLLIRLLKEFGREYTVSG
                     ENAGVHVLVHLPRGLSEAEAVQRAKEDGVRVYGLERYCIGAAADPAQGGTVLLGYAGL
                     SEEEIAEAAAILKKCWNA"
     gene            complement(10409..12082)
                     /locus_tag="PLN77_03425"
     CDS             complement(10409..12082)
                     /locus_tag="PLN77_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRM43366.1"
                     /translation="MEEKEVVSKNFIELAIDKDLAEGVYDHVCTRFPPEPNGYLHIGH
                     AKSILLNYGLSQEYNGEFHMRFDDTNPTKEKTEFVDSIKADIQWLGADWKDHLYYASD
                     YFPQMYEAAVKLIKKGKAFVCDLSAEEIREYRGTLTEPGKESPYRNRSVEENLDLFER
                     MKNGEFEDGSKVLRAKIDMASPNINMRDPVIYRVAHMHHHRTGDTWCIYPMYDFAHPI
                     EDAIEGITHSICTLEFEDHRPLYDWVVRELEYPMPPRQIEFAKLYLTNVVTGKRYIKK
                     LVEDGVVDGWDDPRLVSIAALRRRGFTPESIKKFVDLCGVSKANSSVDYAMLEYCIRE
                     DLKLKKARTMAVLNPVKLVIDNYPEDQIEMLEVQNNLENPELGSRQVPFGRELYIERE
                     DFMEEPVRKYFRMFPGNEVRLMNAYFVTCTGCEKDENGNITVIHGTYDPESKGGNSPD
                     GRKVKGTIHWVAAKTAVKAECRLYENLIDEEKGVYNEDGSMNLNPNSLTVLKECYLEP
                     SLKDAKAYDSFQFVRNGFFCVDAKDSKEDALVFNRIVSLKSSFKLPAAK"
     gene            complement(12234..12722)
                     /locus_tag="PLN77_03430"
     CDS             complement(12234..12722)
                     /locus_tag="PLN77_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM43367.1"
                     /translation="MKHEKSKNDAMRHELDHIDAKILNLLRENARMPLKEIAEHVFLS
                     SPAVSARIERLEKEGYITGYQVKLNPALLGYPIKAFINLEVEPVQKKEFYPYIEKVHN
                     VVECNCVTGDYSMLIEGLFVSTIELDQFIGELQHFGRTRTQIVFSTSVEHRQVTAHEA
                     EE"
     gene            13012..14424
                     /locus_tag="PLN77_03435"
     CDS             13012..14424
                     /locus_tag="PLN77_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRM43368.1"
                     /translation="MKTRIEADSIGSLEVPSDAYYGIQSLRAKRNFPITGTPIHPVMI
                     QNLAKIKKAAAISNREAQRLPEEKAAAIIYACDEIIAGKLKDQFIVDGIQGGAGTSAN
                     MNANEVIANRAIEILGGTKGDYTIVHPNDHVNMAQSTNDVIPSAGKLTVLDLLPDLLH
                     ALESLHAALLDKSQEFDHILKMGRTQLEDAVPMRLGQSFHAYATMIERDIRRIERARK
                     ELFTLNLGGTAIGTTINASDYYLHHIVPTLAAVTGYPLMQADDLFDATENLDGFVTIS
                     NTLKTCAVNLSKMCNDLRLLSSGPRTGFGEINLPPMQNGSSIMPGKINPVIPEVVTQV
                     AFHVIGNDTTITMAAEAGQMELNAFEPVVFYSLFSSITSMTGAVNTLVKNCILGITAN
                     EKRCEDLVSKSVGITTALCPYIGYQKAASIAKKSLKTGESVTSLVLKDELLTQKQLDD
                     ILDPYALTGPELPAEDDLAG"
     gene            complement(14538..>14696)
                     /locus_tag="PLN77_03440"
     CDS             complement(14538..>14696)
                     /locus_tag="PLN77_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HRM43369.1"
                     /translation="MKVAAAYAIAGLVSDEELNADYILPAAFDPRVKDAVAAAVAEAA
                     RKSGVARI"
BASE COUNT         3257 a         4061 c         3527 g         3851 t
ORIGIN      
        1 cgtatttctt cagcatctca ccgccctgta ctttctcgcc ggtcaggata tcttcttttc
       61 cgtcgaagac tgccggaagc ggaatctcct catctttgaa gttgatgata aagtacagat
      121 cctctccgtc tgcctggcgt tttgtgatct cgagtgctgt tgcttctcca atcaccggct
      181 ggatggctgc gccgtctgct gccatagcaa gaacttttgc aaggccgctc tcagaaaggt
      241 cggttccgac ataatatacg gttccttttc cgaactgatt ttttgcaaca gcagtcatgc
      301 ctgcatagaa atcgctctca tactgtgcga tttcttcagc tccttcgagg tgcatcaggt
      361 cacatacaag acggcactct tcctcggttc cgtctgcgaa acggatcttg tttctctgct
      421 ccggtgcaag agcgtcgatc tcttctaccc atacgcctgc catttttctg agcggtcccg
      481 ggtatccgcc gagatgaacg ttgtccgact gtccgacgat accgctcatg taggtggtga
      541 tgagggttcc gcctgcctgt acataagcct ccaggctctc tgccacaccg tcttttacca
      601 tgtagagaac cggtgctgca acgaggtcat atttggagaa gtccgcgtcg atcgggatca
      661 tatcgacagc gatgttcttg ttatagaagt aattgtacat ctgctcgatc tggtctacgt
      721 aacgtaaatc gcggttcggt ccgctggtgt actccagtgc ccagtagttt tcccagtcga
      781 agatgatgcc gacttttgcg tggttctcgg agcccatcac acggcttccg attttctcca
      841 gttcttttcc gagctgggtt acctcacgga agacacgggt atctgcggtt cctgcgtgtg
      901 cgataacagc accatggaat ttctcgcatc cgccgacgct tcttctcagc tggaagaact
      961 gaatcgtgtc tgcgccgtgt gcaacggtct gatagctctg tgcgcgcatc tggcccggtt
     1021 tctttaagga gttgtacggc tgccagttct gctggctcgg agtctgctcc atcagcatga
     1081 acggtgcgtc tttgaggccg cgcatcaggt cgtgggtcat agctacgtgg ctccatggtg
     1141 tattgtagct cgggtagttg tcccaggata cgatatccat ttcttttgcc cacttgaagt
     1201 agtcgaggcc tttgtacgtt cccatcaggt ttgtggtgat cggtgtttct ttgtcgtatt
     1261 tgcggatcgc gtcacgctcc attttgtagt tgttcagcat gccgtcggag ttgaaacgca
     1321 tgtagtcgag ggacattcct gcaaatgcag tgccgcttcc ccatggcatt ccgtctccca
     1381 gatcgttcgg cggtacaatc tcgtcccagt cgtagatggt gtggccccag aactccatgt
     1441 tccatgcctt gttgacttca tcgagtgttt tgtatttgtc tttcagccat acgcggaacg
     1501 cttttgcaca gttctcgcag tagcactcgc cgccgtactc attgctgatg tgccagcaga
     1561 cgatgttttt gttgtcgccg taacgctctg ccagtttttc tgccaggcgt tttgcatatt
     1621 tctggaaaac cgggctgttc gggcaggcat tgtggcgatg accgaatttg tgatggcgtc
     1681 cttcgaaatc ggttcttgcc acttccggat aacggtgtac catccatgcc gggagtgcgc
     1741 cggtggatgt tgccatgacg atctgaacgt cctcttttgt cagggtgtct acgatctcat
     1801 cgagctctgt aaagtcgtac tcattctcgg acggctggat ttttgcccag gaaaatacgt
     1861 tgatcgtggt gctgttgatg ccggcctgat ggaaaagttt catatcctca tcccagatct
     1921 cttttggcca ctggtttggg ttgtagtctc ctccgtataa catttttttg aattttggct
     1981 gcattttttt gattcctcct gtgtttggat atctcgtttt gatggctcta ttatatactg
     2041 gtctttgtga gaaaagtatg atatgcttgt ctaaaaagta tgaattttcg aaactcattc
     2101 cggaatctta ttttagactt ttctttcaga aaggaactga ctgttatgcc tattttcttt
     2161 aaaccatccg cctccgattt ttccctctcc gctgacagca tcggcaaccg ctggctgcag
     2221 gagcccgttg tgcgcccgaa cggattcccc ttatatcact ggattcagac ggaacgcagc
     2281 gaagggctgc tgaccctcga aggtcaggaa cttttgctga aaccaggtga cggtgttctg
     2341 attgctccgc atgtgccgca tgaataccgc gaaaacgacg gcagcgggat gtggtttaca
     2401 tcctttctga cctttgacgg aaagctctcc gatgatctct ataaaatctg tgccgtctcg
     2461 ccgtatcttt tcgttccggc ctcagagggc gcgtggtttc aggaatggat tgaccgcata
     2521 atcgactcac attttctgaa aaatgacatc gacgatgctg cgctctccgt cggatgcttt
     2581 gagttcctga cacgcctttc ccagctccgc accggccttt cccggacgga gcaccctctg
     2641 tatctccagt atgtccgccc ggcaatgcag gaaatcgaaa gtcatctttc cgacccgctc
     2701 aatgtggatg atctcgcgga acagctccat atcacaccgc agtatctgac acgcctgttc
     2761 cagcgttttg tcggcagctc tgtccgctcg tacatcagcg ttctgcgcat cagccgcgcc
     2821 aaggagcttc tcatcagccg tccctacact acgctcgacg gcatcgccgc cctctgcggc
     2881 taccgcgatg tcagctactt tatctcggtt ttccgccagc agaccgggac aacgccgaag
     2941 cagttccgga agctgtacgg catataatct gaaacacccc tctttccggc gccaaaaaag
     3001 atcctccgtc tcatttcaca gacgaaggat cttttttcgt tctctcttat ttcatgcttt
     3061 tcagaatgct tctcgcaacc gacagtccgt ttgcgcttgc ctgctggagg ccgcgggtta
     3121 cggatgcgcc gtcgccgatg gcgcggaggc ctttgacgct ggtgcagaag tctttgtcta
     3181 cgacaacttt gttggaatag aatttgactt ctacgccgta cagcagtgtc tcatcgctgg
     3241 cgatacccgg tgttactttg tcgagggctt cgatcatctc ggcaatgtcg accatgatac
     3301 ggtgcgggaa taccagggaa aggtcgcccg gaacggcatc ttttaaggtc gggatcaggt
     3361 tattgcggca gagacgctct tctgtcgttc ttcttccgcg tttgaaatcg ccgtacgtct
     3421 gaacgaggat cttgccgtcg cagagcatgt tggaaagctg tgcgatctgt ttgccgtact
     3481 cgatcggggt tttaaatggt ttcgtaaagt tcttggatac caggagggca aagttcgtgt
     3541 tgtttgtctt gaactcctgg gatttgtagg cgtgaccgtt tacaactgcc agaccgtttt
     3601 cataatactc agttgccacc tcaccggacg ggttcgtaca gaaggtacgc actttgtcgt
     3661 caaaagtcgg ggtatagtaa acgagttttg cctcgtacag gttcttgttc aggaactcca
     3721 tgacctcgtc gcggacttcc acgcggacac cgatatcgac ggttccgacc tcggtctcga
     3781 tgccgtggcc gttgcagata tgtgagaacc agtcggctcc ctcgcggcct actgcggaga
     3841 caatctcatc tgcctcgtaa accgttccgt cctcaagtgt tacagctgtc gcacggccgt
     3901 cctcgatcgt aatatcattg accatcgtgt tgaatttcat ttccacgccg atctctttta
     3961 agtgatcctc gaggcggctg taaatcttgt agccctcctc agttccgaga tggcggattg
     4021 gacattcgat cagcttcagg ttggcgttga tcgctctgcg gcggatctca cggatctctt
     4081 tttctttatc gacaccgtaa acattcttgt ctgcgccgaa atccagatag atctggtcgg
     4141 actcatggat cagctcaacc gtcttttcat atcctaaaat ctccggcaga tttccaccga
     4201 catccgggga aagagaaagc tttccgtcgg agaatgcgcc tgctccggca aatcccgttg
     4261 tgatcgagca cggtttgcag ttcacgcatt ttttcgtttt tctctttgga cactgacggc
     4321 tctcgatcga acgtcctttt tcgatcatca gcaccttcag atccggtttt tctttcagca
     4381 gttcataggc acagaaaata cctgacgggc ctgcgccgat aataatgaca tcatatttca
     4441 ttctttttta atttcctctt gttttttatc ccatcacgat cagatactga ccgtccggca
     4501 gggcaaagac ttcgctctgc tcttctaatt ctgcatccga catttcttcc acgtccatgc
     4561 cctcttcttc catgtaggcg cgcacgtcgt ccagattgtc ggcaacgact gcaaggcagt
     4621 cctccagaaa ggcctctgcc tcatcgtaat tttcagccac cggctcgtcg aataactggc
     4681 cctgattcat taaaaatgtg ttgatgcatt cttcagtata ttctcccatt ctggttttct
     4741 cctctgttcg ttttattttt cctgattctt tctgtgaaaa agcgttaaga tttcctccgg
     4801 ccggtcgagc agctccatcg catggttgtc gatcagctcc tggcgcgggc ggaatcccca
     4861 cgtcacacca acggtaaaca tgcctgcggc gtttccggtc tgcatatccg tattggtatc
     4921 gccaaagtac aggcactctt ccggtctggc gccgagtgcg tctgcaatcg cgagcgcacc
     4981 gatcggagac ggctttctcg ggatttctgc ggtctgcccc tgaatccggt tgaaaaagcc
     5041 cggtccgtac acgctctcca cgacgtggat ggccgcctcg tgcggcttgt tggaacatac
     5101 ggcaatcgga acttcttccc gcagcgcggt cagggtttcc cgcatattag gatacggttt
     5161 tacgtgatac agcgggtcct cgttgaacca cgcgcggcca aggcggatgc cctcctgcag
     5221 atagttgagc tctgcatctc cggcgtcttt taaagcgcgc tccactgcca gatcgaaacc
     5281 gtctccggcg taaaagttga aggcttctac cgggcgcggc acaagtccaa aatgttccag
     5341 aacgcggttg actgcgtgcg caatcgactc tacagtgtct gcgatggtgc cgtccaggtc
     5401 aaagatacaa gcttttatca tattctgact tcccttcatt gtctcttttg ttcttcgatt
     5461 ttctttcttc tctctcttct tgtccgcaat cattatatcc tgtatttttt ccctgcgcaa
     5521 ccatattttc ttttccggca ctttatgcta aaatgaaact gtttttcaga ttcttggaaa
     5581 ggaaaaaaag agaatatgaa tcagactgtg aaaatatttt ttaccgatat ggacgatacc
     5641 ctgctaaatt ccgaaaagca ggtgacaaaa gaaaacatgg atgccatttc agaggcactt
     5701 gctgccggcc acaaaattgt gttcaacacc ggccgcccgc tctccggctt tctgccgatc
     5761 ttaaaagagc tcggacttga ccgcgaaggc tgctatgcca tcgctttcaa cggcggactg
     5821 atttacgact gtgctgcgca aaaaacgctg caccgccgca cgattccgct cgaatatgca
     5881 cgcgtcattt tcgaaaaaac gcaggaggca ggcctctact gccacaccta ctccgacacc
     5941 tcccttctct gccgccatgc aggcgaggaa acgcgcgttt acacacaggc aatccacatt
     6001 cccgtcgtgg aagatccgct tctgccggaa cacctcgccg aggaaccatg caagatgatc
     6061 gtgattgatc tccacgaccg tgcaaaaatg gacgcttacc gcgctgccat ggagccgtgg
     6121 gcaaaaggaa aaatttcgat gttctactcc agcccgcatt acctcgagca tgtcccggaa
     6181 cacgtttcca agggccatgc cgtgaaaaaa ctctgcgagc ttcttcagat tccgcttgcc
     6241 aacaccgtgg ctgccggaga tcaggaaaat gatatttcga tgatcgaggc cgctgccgtc
     6301 ggtgccgcca tgaaaaatgc gaccgatgcc gtaaaggcga gtgccgatta tatcacggaa
     6361 aacgactgca atcacagcgg cgtggcggag attcttcgta agtttatcct tgcctgattc
     6421 tgtttctgtg tcaggaaacc ttctttttct tgttttcttt ggcacttttt ccgaattctg
     6481 taagatttta ctttcttttt tgttgaaact gtattacaat agtttcaaag gacggcattt
     6541 gaaaggagac ttgaatcgag atgtcacgga aatcaaaacg tattctgctt actctggctt
     6601 ctgccgctgt cggcgcagcc tgcggtgcgg cagtctattt tatccggaaa ggaaaagcag
     6661 atcttgaaac agattttgac gattttaaag acgaatttga agaggaagag cctgctcctg
     6721 ctgaacgtac ttatacgacc cttccgaaaa cgaaaggtgc ggaggatgct gcaccggatc
     6781 cggaatctgc agcttgcaat gaaggttccg aaacagctga tgcagaagct gaaagcgaaa
     6841 ccgcagagtc cgaagaagaa gacgctgaaa aagaaagctg tgcggatcgt gaagtttctc
     6901 cgcaggaaga aagcgaagta accgcagaat aaaacccaaa aacagaatat agaaacataa
     6961 ggaagtaatt cctggggagg gaaacctccc cttttttatg gaaaggagat tgttatggaa
     7021 cttgcaaaac gcgatgacgt tcctgtagaa cttacctggg acctgtcttt aatttatcca
     7081 accgaggagg ccatgctggc tgatgcgcag aaaatgaaag aattaagtct ttctatggaa
     7141 gcatcctaca aaggaaacct caccgatgca gccacgatca accactgcct cgacgattac
     7201 caggaggtct accgtctgat cacactgacc gccaactact gcgatctcgc cgtctctgtc
     7261 gactattaca acagtgcaaa ccagacgcgc aacgaccgca tcaacagcct gatctctgag
     7321 attttcagcc gtctcacctt tatcgagagc gagctttccg aacagtccga ggatgttttg
     7381 aacgaggcca tgcagcagtc agatacgaac cgctgctatc tcgccgagat tctccgcaac
     7441 aaggaacacc gcctgaatcc ggaaaccgag cgtgccattt ccgctctgtc ccagaccttc
     7501 tcggctccgt accagatcta caacatggca aagcttgccg atatgaaatt tgattctttc
     7561 accgtaaacg gaaaagaata tccgctcggc tactccctgt ttgaggataa ttacgagtac
     7621 gaaaaagaca ccgatatccg ccgcagtgca ttctccgcct tttctgcaaa aatcagacag
     7681 tacgaaaatg taacggcagc tgcttacaat gcccagctgc agacagaaaa gaccatggcc
     7741 acgctgcgcg gcttcgattc cgtcatcgat tctctgctgt tcccgcagca ggtcagccgt
     7801 gagctgtaca accgccagat cgatctcatc accacccgcc tggctccgca tatgcgcaaa
     7861 tatgcccgcc tgctgaaaaa ggttcacaac cttgaccgca tgaccttcgc cgatctgaaa
     7921 atcgccgtcg acccggagta cgatccgtct gtcacgatcg aggaatcaaa acagtacatc
     7981 gaaaaaggcc ttgccattct cggtgatgat tacgtttcca tgattcagga ggcctacaaa
     8041 aaacgctggg ttgactttgc acagaaccag ggaaaatcca ccggcggctt ctgtgccagc
     8101 ccgtacggaa aaggttcctt tattctctta agctggaata accgcatggc agatgttttc
     8161 acgctggcgc atgaactcgg ccacgccggc catttccgtc tctgcaacgg cgcacaggcg
     8221 attctcgata ccgaggtttc ctcttatttt gtcgaggcgc cgtctaccat gaacgagctt
     8281 ctgatggctc attatctgct gaaaacgacc ccggacaaac ggttccgccg ctgggtttta
     8341 agctgtatga tcagcaacac atattatcat aacttcgtca cccatctgat ggaggctgct
     8401 taccagcgcg aggtttacaa gctcatcgat gccggcgaca gcgtacaggc tgagacgtta
     8461 agcagcatta tgaaagagac cctgcagaag ttctggggcg atgatgtgga aatttccgat
     8521 gacgcagcgc tcacctggat gcgccagccg cattactata tgggacttta ctcctacacc
     8581 tacagcgccg gactgaccgt tgccacacag gtctgcaagc gcatcgaaac cgaaggccag
     8641 accgctgtcg atgactggaa aaaggtactg accgccggaa gcacgaagac accggaagag
     8701 ctggcaaaaa tggccggcgt tgatatctcg acggatgcac cgcttctcga tacgatcgag
     8761 acgattggat cgatcatcga tgagatctgc gagctgaccg aagagctggg ctgctgatcc
     8821 tgtccgcatt gtaactctct atcaaacagt aaaaacggat tgttccgtca gtttttgtta
     8881 ctggcgaagc aatccgtttt tttcatatct tttttcacgc attccagcac tttttcagaa
     8941 tcgcagctgc ctctgcaatc tcctcctcag aaagcccggc gtacccaagc agcactgtgc
     9001 cgccctgtgc gggatctgcg gcagcgccga tgcagtaccg ttccagcccg tacacgcgca
     9061 cgccatcctc ttttgcccgc tgcaccgcct ccgcttcgga gagtcctctc ggcaggtgga
     9121 ccagcacatg gacgcccgca ttctcgccgg acaccgtgta ctcccgccca aactccttca
     9181 gaagccggat cagcaggtca tgtttccctt tatactgtgc acgcatccgg ttcagatggc
     9241 gctcgaacgc gccgctccgc ataaactctt ccagaatctt ctggtccgtc ttcgaaactg
     9301 tcgcggaaaa gcatcttccg ttctcctcgt aggcaggcag cagcgtcttc ggcagcacca
     9361 tgtagctgat gcggatggac ggcgcaatcg cccgcgaaaa cgttccaaga tagatcactt
     9421 ttccctctgt gtcattgccc tgcagtgccg ggatcgggcg gcccttgtag cggaattcac
     9481 tgtcgtaatc atcctcaatc agataccgcc cattttccct cgatgcccac gcaaggagct
     9541 gcatccgccg cttcatcggc atgaccatgc ccatcgggaa ctggtgcgac ggcatgatgt
     9601 acgccagatc cgccccgctt ttttccagct ccgccggccg catgcccgct tcgtccatcg
     9661 aaatcgtaca catcgcatag ccgagcttcg caaaacagcg gtaggcactg atgtaggtcg
     9721 gattttccat cgccacttta tggccacgcc ctaagatcac cgaaagcagc atcagcagat
     9781 aatcatttcc cgctccgacg atgatctgct ccgtgcggca gttcacgccg cgtgcgtggt
     9841 gcagatagtc gcggattgcc tcccgaagac cgtgctcgcc cttcgcatcc ccgagctgga
     9901 acaggctgtc atctgcgtcc agcaggactt ctttcgagat cttcttccag ctgttctgcg
     9961 gaaatccgcc cggcgccgta ccggtcacgg caaaatcgta gcggaacggc atctgttccg
    10021 cggcatcatc ctcttttgtc tcctctttct tttcatcgag gcggtacagc ccctcaatct
    10081 cacagacaaa gtagcctttg cacggcacac tctccagata cccttcggat accagctgct
    10141 catacgccag atccacggta ctccggctca ccgacagata gcccgaaagc gcccgcgccg
    10201 acggcagccg ctcccctgca cggatccgtc cgccgcggat ttcctcgcgg ataaaaccgt
    10261 agatctgctc atagagcggt gtcttccgat gcggatccag cgaaatcgtc aattcattca
    10321 tcttcccatc tcccctgaca aaaagaacag ccgcttggga tgtcggtttc atccaggcgg
    10381 ctgctctgtt ccatttcaaa attcttattt atttcgctgc cggcagcttg aaagagctct
    10441 tcagagatac gatacggttg aacaccagcg catcctcttt ggaatctttt gcgtctacac
    10501 agaagaatcc gtttctgaca aactggaagc tgtcgtatgc tttcgcatct tttaaagacg
    10561 gctccagata gcactctttt aatacggtca gggaattcgg attcaggttc atggaaccat
    10621 cctcgttgta aacgcccttc tcctcgtcga tcaggttttc gtacagacgg cactctgctt
    10681 tgacggcggt ctttgctgct acccagtgga tcgttccttt tacttttctt ccgtccgggg
    10741 aatttcctcc cttggactcc ggatcgtacg ttccatggat tacggtaata tttccgttct
    10801 cgtctttctc gcatccggtg caggtgacga agtatgcgtt catcaggcgg acttcattgc
    10861 ccgggaacat acggaagtat ttgcggaccg gctcctccat gaagtcttcg cgctcgatgt
    10921 acagttctct tccgaacggt acctgacggc tgccaagctc cggattctcg aggttattct
    10981 gtacttccag catctcaatc tggtcttccg gatagttgtc gatgacaagt ttcaccggat
    11041 ttaagactgc catggtacgg gcttttttca gcttcagatc ctcgcggatg cagtactcca
    11101 gcatcgcata gtcaacggag ctgtttgctt ttgatacacc gcacagatct acgaatttct
    11161 tgatggactc cggtgtaaag cctctgcggc ggagtgctgc gatggatacg agacgcgggt
    11221 catcccagcc gtctaccacg ccgtcctcta ccagcttttt gatgtaacgt tttccggtaa
    11281 ccacgttggt cagatacagc tttgcaaact caatctggcg cggcggcatc ggatattcca
    11341 gctcgcgtac aacccagtcg tacagcggac ggtggtcctc aaactccagc gtacagatag
    11401 aatgggtaat gccttcgatg gcgtcctcga tcggatgcgc gaagtcgtac atcgggtaga
    11461 tgcaccaggt atcgccggtt ctgtggtgat gcatatgggc tacacggtag ataaccgggt
    11521 ctctcatgtt gatgttcggt gatgccatat cgattttcgc acggagtact ttgcttccgt
    11581 cctcgaattc gccgtttttc atgcgctcga acaggtccag gttttcctct acggaacgat
    11641 tgcggtacgg gctctctttt ccaggctcgg ttaaggttcc gcggtactca cggatctcct
    11701 ctgcggacag atcgcagacg aatgcttttc cttttttgat cagttttacg gctgcctcat
    11761 acatctgtgg gaaataatcg gatgcgtagt acagatgatc tttccagtct gcaccaagcc
    11821 actggatatc ggctttgatg gaatcaacaa actcggtttt ctctttcgtc gggtttgtat
    11881 catcgaaacg catgtggaac tctccgttgt actcctgtga taaaccgtag tttaacagga
    11941 tggattttgc atgtccgata tgaagatagc cgtttggctc cggcgggaat cgtgtgcata
    12001 catggtcgta aacgccctct gccagatctt tgtcaatcgc aagctcgata aaatttttgg
    12061 aaacaacttc tttttcttcc ataataaggt caggcctccc tcgaatcttt tcgtttggag
    12121 cactgccccg aaaagggcgc aaaatgctct gtgtattcta tcactataac acaaaagcat
    12181 tctcctgcaa agcatttttt tcggtacaca aagccgccgg agcggaccgg tgcctattct
    12241 tctgcctcgt gtgcggtcac ctggcggtgc tccacggagg tcgagaacac gatctgcgtc
    12301 cgcgtccgtc caaaatgctg cagctcgccg ataaactggt cgagctcaat ggtgctaaca
    12361 aaaaggccct caatgagcat ggagtaatca ccggtaacgc aattacattc cacgacattg
    12421 tggacctttt caatataggg ataaaattct tttttctgaa ccggctcaac ctcgaggttg
    12481 atgaacgctt tgatcggata tcccaaaagc gccggattta atttgacctg atatcctgta
    12541 atgtagcctt ccttctccag ccgctcaatt ctggcggaaa ctgccgggga ggataaaaat
    12601 acgtgttccg cgatttcttt cagcggcatc ctggcgtttt cccgaaggag attcaggatt
    12661 tttgcatcga tgtgatcgag ctcatggcgc atcgcatcgt tcttgctttt ttcatgtttc
    12721 atacgctgct ctcctgtttt ttcactttta tactgttcag catatcacgt tcgccgtttt
    12781 ttgtctacga aaacgtgtcc tcgcggaaac tgctttcgaa aaataagtaa aagtgcctct
    12841 ttcccttggt atttaaaatc aaatgcaaaa ataaataaga ttccacagtt cttcggcggc
    12901 ggtatttaca aatttctgcc ctggtgttat tcttccggta tcgaaaggca aagcaagaat
    12961 tgtttcagac aatatacaca actcaatgcg tgcagaaaga aggagattat tatgaaaaca
    13021 agaattgaag cagattccat tggttccctt gaagtcccct ccgacgccta ctacgggata
    13081 cagtccctgc gggcaaaacg gaatttcccc atcacgggaa cgccgatcca cccggtcatg
    13141 atccagaatc tggcgaaaat taaaaaggca gctgccatct ccaaccgcga agcacagaga
    13201 ctgccggagg aaaaagcggc tgccattatc tatgcctgtg atgaaatcat tgccggcaaa
    13261 ttaaaagacc agttcatcgt tgacggcatc cagggcggcg ccggaacgag tgctaatatg
    13321 aacgcaaacg aagtcatcgc aaaccgcgcc atcgaaatcc tgggcggcac caagggtgat
    13381 tataccattg tccacccgaa tgaccacgtc aacatggcac agtccacaaa cgacgttatc
    13441 ccaagtgcag gaaaactgac cgtgctcgat ctgctcccgg atcttctgca tgcgctcgag
    13501 tcactccacg cagcactgct cgataagtcc caggaatttg accacatttt aaagatggga
    13561 agaacccagc ttgaggacgc cgttccgatg cgtctcggcc agagcttcca cgcttacgca
    13621 acgatgatcg agcgtgatat ccgccggatc gaacgcgcca gaaaagagct gttcaccctg
    13681 aacctcggcg gaacagccat cggtacgacg atcaatgctt ccgattacta tctccaccac
    13741 atcgtgccga ccctcgctgc tgtgacaggc tatccgctga tgcaggcgga cgatctgttt
    13801 gacgcgacgg agaacctcga cggatttgtg acgatctcca acacgttaaa gacctgcgcc
    13861 gtcaacctct ccaagatgtg caacgacctg cgcctgctgt ccagcggacc gcgaaccgga
    13921 ttcggcgaga tcaacctgcc gccgatgcag aacggatcct cgattatgcc gggcaagatc
    13981 aacccggtta tcccggaggt tgtgacacag gttgccttcc atgtcatcgg aaacgacacc
    14041 acaatcacga tggcggctga ggctggccag atggagctga acgcgtttga gccggttgtc
    14101 ttctactccc tgttctcttc catcacctcg atgacaggcg ctgtgaatac gcttgtgaag
    14161 aactgtattc tcggcattac cgcaaatgag aaacggtgcg aggatctcgt ttcgaagagt
    14221 gtcggcatca caacggcgct ctgcccgtat atcggatacc agaaagcggc ctcgattgca
    14281 aagaaatcgc tgaagacggg cgagagtgtt acttctctgg ttctgaaaga cgaactgctg
    14341 acacagaaac agctcgacga cattctcgat ccgtacgctc tgaccggacc ggaactgccg
    14401 gcagaagacg atctggctgg ctgaatgtaa ctgctgcttt ccttcataat ttcttaacga
    14461 tacgcaaaaa ggcttcccgg atttccttgc tgtccgggaa gcctttttct gtcgttttct
    14521 tttgtatcaa actctgttta aattctcgct acgccgctct ttcttgctgc ctctgcaacg
    14581 gctgctgcca ccgcatcttt gacacgcgga tcaaacgctg ccggcaggat atagtctgca
    14641 ttcagctctt catcgcttac caggcctgcg attgcgtaag ctgctgcgac cttcat
//
LOCUS       DAOMUF010000033        14592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_874_length_14592_cov_3.400358, whole genome shotgun sequence.
ACCESSION   DAOMUF010000033 DAOMUF010000000
VERSION     DAOMUF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 14592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14592
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_874_length_14592_cov_3.400358"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="scfB"
                     /locus_tag="PLN77_03445"
     CDS             <1..843
                     /gene="scfB"
                     /locus_tag="PLN77_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HRM43370.1"
                     /translation="QEKLHNKNFRFTLTTNGVLLNDEVMEFCNKEMGNVVLSIDGRKE
                     VHDHMRPFRKGAGSYDLIVPKFQKFAELRHQDKYYVRGTFTHYNTDFAADVLHLADLG
                     FKQISVEPVVAPPTEDYAIREEDLPVIFEQYDILAREMIKREKEGRGFNFFHFMIDLT
                     GGPCVYKRLSGCGSGTEYLAVTPWGDFYPCHQFVGQEDFCLGNVYEGVKKTETVNEFK
                     KCNVYSKKECSQCFARFYCSGGCAANSYNFTGKINDVYEIGCKMQQKRIECALMIKAA
                     LAED"
     gene            868..3147
                     /locus_tag="PLN77_03450"
     CDS             868..3147
                     /locus_tag="PLN77_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRM43371.1"
                     /translation="MKKKQSIIALVLAAIVTVLLGWTMIKGWGPTGTGSMKNINLGLD
                     LSGGVSITYQAVKDNPTDEEMSDTRYKLEQRAQQYSEEAQVYLQGDNRITIEIPGATD
                     ATTILEEMGKPGSLYFIKQTNDDGTENYTYDSSTGEYVLNGKTIEELEEDGSVVLTGK
                     DVESAEAMHQQNSTTKATESVVQLKMTDEGKQKFADATQEAYSAGKSIGIYYDEKFVS
                     VPSVNAVISDGTAVISGGNMDWDEATSLASTLRIGSLSLKLEEINSSVVGAQLGSAAV
                     STSVKAGAIGIILIIIFLAVVYRLPGIAAGWALLIYVELNLISLNAFDLTLTLPGIAG
                     VILSIGMAVDANVIIYARIEEEIGAGKTVHAAIRDGFKKAASAILDGNVTTLIAAAVL
                     YALASGSVRGFAMTLALGIVLSMFSAMVVSRLLVNSLYGMGLKDAKYYGTKKERKGFP
                     FVEKRKIFFTISCILLLAVPASMIFMHQTKGSALNFGLDFKGGTSINVPFNEDYSIEE
                     LDKEVEPVVEGVTKDSNIQMTKVVGGNNVIIKTRSLTLEEREQVYQAMADNFGVDTSE
                     ITFDNISSTVSKEMSQNAMKAVIIAVVCMLLYIWIRFRDIRFASSAILALMHDIAIVF
                     GFYVVSRISVGSTFIACMLTILGYSINSTIVIFDRIRENLPELKREPIESLVDRCITD
                     TLTRSIYSSLTTFITIFVLFVMGVSSIRDFAAPLMVGIVCGAYTSVCITGALWYVMKH
                     VGKNKYVPSKAVQNKTKNK"
     gene            3227..4951
                     /gene="recJ"
                     /locus_tag="PLN77_03455"
     CDS             3227..4951
                     /gene="recJ"
                     /locus_tag="PLN77_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRM43372.1"
                     /translation="MKKERWVVSAKRADFQAIADRFGIDPVIARLIRNRDVTGEKEIE
                     EYLFGDLEQLHDPLLMKDMERAADLIAEKIREKKPIRIIGDYDIDGVTATYILLTGLK
                     DLGADVDVRIPDRIADGYGLNEHLVQFAADEGRDTIVTCDNGIAAGSQIRLAKELGMT
                     VVVTDHHEVPFEEDENGERRYILPPADAVVNPKQKDCSYPFPGLCGAAVAWKLIQTME
                     AKAGIPKEHSFRFLEFVAIATIGDVMDLQGENRIFVKAGLRALHKTQNLGLQELIRVQ
                     GIEAENVTPYHIGFVLGPCINASGRLDTAEHSLKLLCAKTREEAAKLAGDLKDLNESR
                     KELTRQGEAKAIELVETSPLQNDKVLVVYLPDCHESLAGIIAGRLREHFHKPSFVLTR
                     SEEGVKGSGRSIEAYSMYEELCKCKELMTKFGGHPMAAGLSLAGEEMIEPFRRALNDN
                     STLTEEDFVEKVVIDVPMPITYITKNLIRQLSLLEPFGKGNTKPLFAQKGLTVLNYRI
                     FGKNRNVVKVQLADAGGYQMDGVYFGEGEAFAAYVDAHPALSVAYYPSIDTWNGRDKL
                     QINIQSYF"
     gene            5083..5607
                     /locus_tag="PLN77_03460"
     CDS             5083..5607
                     /locus_tag="PLN77_03460"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRM43373.1"
                     /translation="MKKVEDYVRSIPDFPEPGIIFRDVTSILQDADGLQLAIDEMQHF
                     VEEVDCDVICGTESRGFIFGMPIAYNLHKPFVPIRKKGKLPLETVEESYDLEYGSATI
                     EMHKDSIKPGQKVVIIDDLIATGGTVEACAKMIERLGGEVTRIVFLMELAGLKGREKL
                     AKYDVKSVIRYDGK"
     gene            5655..7952
                     /locus_tag="PLN77_03465"
     CDS             5655..7952
                     /locus_tag="PLN77_03465"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRM43374.1"
                     /translation="MEEEKRISEEELKKDVAAAEARIRKTDDFTSPEELYGELIASVK
                     KYHPSDDISLIEKAYRVAADAHKDQKRKSGEPYIIHPLCVAIILADLELDKESIAAGL
                     LHDVVEDTVMTEEELTEEFGPEVSLIVSGVTKLAQLKYSADKVEEQAENLRKMFLAMA
                     KDIRVILVKLADRLHNMRTAQYWSPETQKKKARETMDIYAPIAQRLGISKIKVELDDL
                     SLKYLEPDAYYELVQKVSMKREQRQEFVDAIVKEVTRHIHQAGIEADVNGRIKHFFSI
                     YKKMVNQHKTLDQIYDLFAVRIIVESVKDCYAALGVIHEMYTPIPGRFKDYIAMPKPN
                     RYQSLHTTLIGPNGQPFEIQIRTYEMHRIAEYGIAAHWKYKEASDGKKPGPEQEEEKL
                     TWLRQILEWQQDMADNHEFMNLLKSDLDLFADNVYCFSPAGDVKTLPVGSCAIDFAYS
                     VHSAVGNKMVGARVNGKLVPIETELHNGDRVEIITSQNSKGPSRDWLKIAKSSQAKSR
                     INQWFRQQNKTDNIVKGKEMLTNYAKLKGKNLGIYTKPMYEEAVMRKYGFRDWDSVLA
                     AIGHGGLKEGQVLNKLIETYEKQHKKEITDEQVLEAASGEKVLPVGKSKGGIVVRGIH
                     DVAVRFSKCCNPIPGDEIVGFVTRGRGVTIHRTDCVNVLNLPESERARLIDAEWQAED
                     NGGSLYTVEISVYANNRTGLLVDISKIFTERKIDLAAMNVRTSKQGTASIDVTFDVHN
                     NEELNSIIEKVRQVESVIDIQRSRG"
     gene            7968..8591
                     /locus_tag="PLN77_03470"
     CDS             7968..8591
                     /locus_tag="PLN77_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM43375.1"
                     /translation="MIKVNYRALGMLSTNCYVVENPDSQKVLIVDPGDSPLEIERQID
                     EIDGDPEAILLTHGHFDHMLAADALRKKYHIPVYVHEKEQHLLGDPKENLSGLWSKPY
                     TMQADKTVKEGDVLHLADFEIHVLATPGHTAGGVCYYIPAEKALFSGDTLFCESYGRY
                     DFPTSSGRELIASVKRLLKELPGDVTVYPGHNDETTIEHEKRYNPLA"
     gene            8563..10062
                     /gene="hemZ"
                     /locus_tag="PLN77_03475"
     CDS             8563..10062
                     /gene="hemZ"
                     /locus_tag="PLN77_03475"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HRM43376.1"
                     /translation="MKRGTIPLLNISLCFNRKDFEYDVYSLIKAFYPGCEITSWYEED
                     GALDGEFAYYDTITYEADQICFSIADEKHETLASQCEAVEYEKDRHETKNVLKRMVYR
                     TLSEVSGKELPWGDLTGIRPTKIPMKMLEEGKKNVEIAKYMRETYYTSPEKTALAITI
                     ANREKDILKTIDYEHGYSLYIGIPFCPSICLYCSFGSHVLSRWEHMVDPYLDALIKEL
                     IFISENMKDYTLDTIYIGGGTPTTLNAAQMERLLTKVTELFPMEQVQEFTVEAGRPDT
                     INEEVLKAIRKFPVTRISINPQTMNQETLDLIGRHHTVEEIEEKFRMARSLGFDNINM
                     DLIVGLPGEDKEKVAHTLEKVEALNPDSLTVHSLALKRATRLNLFKDKYQEISFENSA
                     EIMKMTMDSAHRMEMGPYYMYRQKNMAGNFENVGYSREGKAGIYNILIMEEKQSILAA
                     GAGASTKFVFEHGERIERVENVKDLKNYVERIDEMIERKRIGMEKYLPK"
     gene            10059..10739
                     /locus_tag="PLN77_03480"
     CDS             10059..10739
                     /locus_tag="PLN77_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM43377.1"
                     /translation="MKENSSQQLHMLRYTLTEQLIHGMEVSNLAYDLARELGYEKEIC
                     YELAKAGVLHDIGKIVLENYVEEQDTLVVEEMRFVRTHSTLGYELLQGRGYSDFVLES
                     ILYHHENYDGTGYPANLAGEKIPFGARILRICDVYCALTSDRPYRSAFPREQAMELMT
                     EEVKNFDLKMFLAFQRVIHSGSRKAIELSDVDELIREIIKEKTENGIKEETGYRNERY
                     FTERNGNP"
     gene            10672..11928
                     /gene="hisS"
                     /locus_tag="PLN77_03485"
     CDS             10672..11928
                     /gene="hisS"
                     /locus_tag="PLN77_03485"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRM43378.1"
                     /translation="MALKKKPVTGMKDILPKEMAIRDYVIRLIKETYGTFGFTSMETP
                     CVEHIENLNSKQGGENEKLIFKILKRGEKLKLETAEKENDLVDSGLRYDLTVPLCRYY
                     SNNANELPSPFKALQMGNVWRADRPQRGRYRQFMQCDIDILGEPTNLAEIELILATTT
                     LLGKLDFKNFTIRINDRRILKAMAAYSGFPEESYDTVFIILDKMDKIGFEGVAKELEE
                     AGFAKESVEKYLKMFEEITPDTAGVEYCREKLEGFLDKEYADGLKTIIDSVNAVKTAE
                     FKIAFDPTLVRGMSYYTGPIFEIAMDEYGGSVGGGGRYDEMIGKFTGNQTCACGFSIG
                     FERIVMLLLERDYQIPGAQKKKAYLIEKNMPAEGMQKIIQQATKERSEGTAINIAVMK
                     KNKKFQKEQLASEGYTEIEEFFVDKM"
     gene            12021..13829
                     /gene="aspS"
                     /locus_tag="PLN77_03490"
     CDS             12021..13829
                     /gene="aspS"
                     /locus_tag="PLN77_03490"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRM43379.1"
                     /translation="MAESMKGLKRTCRCAEVTKADIGKKVTLMGWVQKSRNKGGIIFV
                     DLRDRSGIMQIIFENGPISEEGFEKAAKLRSEFVIAVEGEVMARSGAVNENLATGELE
                     VKAESLRILSEAETPPFPIEEDSKTREELRLKYRYLDLRRPDIQRNLIMRSKVAMLTR
                     EFMSKEGFLEIETPMLTKSTPEGARDYLVPSRVHPGSFYALPQSPQLFKQLLMCSGYD
                     RYIQIVKCFRDEDLRADRQPEFTQIDMELAFVDVDDVIDVNERLLAHLFKEVLGVEVQ
                     LPIQRMTWKEAMNRFGSDKPDLRFGMELVDVSETVKDTEFVVFKGALENGGSVRGINA
                     KGQGAMPRKKIDKLVEFAKGYGAKGLAYIAIHEDGSWKSSFSKFMTEEQMEALIKAMD
                     GQAGDLLLFAADRNKIVWNVLGALRLELAEQMGLLDKNEYRFVWITEFPLLEWSDEEN
                     RFTAMHHPFTMPMDEDLPLLDTDPGAVRAKAYDIVLNGTEIGGGSVRIHQNDVQEKMF
                     EMLGFTKEQAHERFGFLLDAFKYGVPPHAGLAYGLDRLVMLMAKEDSIRDVIAFPKVK
                     DASCLMTEAPSPVDAAQLEELGLEVEPQAEEETTEE"
     gene            14053..14316
                     /locus_tag="PLN77_03495"
     CDS             14053..14316
                     /locus_tag="PLN77_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43380.1"
                     /translation="MLVTLVMLATCFGLLCGIGILFGIVGLLLKFGGKVLGACATILL
                     PIGVIWLGFKIVFAVVPLLFKLVCAALICYLVYRILREMKLVA"
BASE COUNT         4095 a         3440 c         3958 g         3099 t
ORIGIN      
        1 caggagaagt tacataacaa gaacttccgt ttcaccctga ccaccaacgg tgttctctta
       61 aacgacgagg tcatggagtt ctgcaataag gagatgggca acgtcgtatt aagtatcgac
      121 ggaagaaaag aagttcacga tcacatgcgt ccgttccgca aaggtgccgg aagctacgat
      181 ctgatcgtgc cgaaattcca gaaattcgct gaactccgtc atcaggacaa atactatgtg
      241 cgcggaacct tcacgcatta caacacagac tttgcagctg atgttctcca tctggcagac
      301 ctcggcttca agcagatctc ggttgagcct gttgtagcac ctccgaccga ggattacgcg
      361 atccgcgagg aagaccttcc ggtgattttc gaacagtacg acattctggc aagagagatg
      421 atcaagagag aaaaagaagg aagaggcttc aatttcttcc atttcatgat cgatctgacc
      481 ggcggaccat gtgtttataa acgtctttcc ggctgcggtt ccggaaccga gtaccttgcg
      541 gtaacaccgt ggggcgattt ctatccgtgc caccagttcg taggacagga agatttctgc
      601 cttggaaatg tttacgaagg cgtgaaaaag acagaaacgg tcaacgaatt caagaaatgc
      661 aacgtttatt ccaagaagga gtgcagccag tgctttgcac ggttctactg cagcggcgga
      721 tgcgcggcaa actcctacaa tttcacaggt aagatcaacg atgtttacga gatcggatgc
      781 aagatgcagc agaaacggat cgagtgtgca ttgatgataa aggcagctct ggccgaagat
      841 taaagaaaag aggaaaatcg cgacatgatg aagaagaaac agagtatcat cgcactcgtc
      901 ctggcagcca ttgtaacagt gcttcttgga tggacgatga tcaaaggatg gggtcctacc
      961 ggaaccggtt cgatgaagaa catcaacctg ggtctggatc tgtccggcgg tgtcagcatt
     1021 acctatcagg ccgtaaagga caacccgaca gacgaagaaa tgtcagatac ccgctacaag
     1081 ctggaacagc gtgcgcagca gtacagcgaa gaggcacagg tatatctgca gggcgacaac
     1141 cgtatcacca ttgaaatccc gggtgcaacc gatgcaacca ccattctgga agagatgggt
     1201 aaaccgggaa gcctgtattt catcaaacag accaacgatg acggaacaga aaactacacc
     1261 tacgacagct cgaccggcga gtacgttctg aacggaaaaa ctatcgaaga gctcgaggag
     1321 gacggctccg tcgttctgac cggtaaagat gtggagagcg cagaagccat gcatcagcag
     1381 aactccacca caaaagcaac cgagagcgtc gttcagctga aaatgaccga cgaaggaaaa
     1441 cagaaatttg cagacgcaac ccaggaagcc tacagcgcag gaaagagcat cggtatctac
     1501 tatgatgaga aattcgtcag tgtaccgagt gtaaacgctg tgatttccga tggtactgca
     1561 gtcatctccg gcggaaacat ggactgggat gaagcaacca gcctggcatc caccctccgt
     1621 atcggttccc tttccctgaa acttgaggag atcaactcca gcgtcgttgg agcacagctt
     1681 ggatctgcag ctgtttccac cagcgtgaaa gcaggagcga tcggcattat cctgatcatc
     1741 atcttccttg cagttgtata ccgcctgcct ggtattgcag ccggatgggc actgctcatt
     1801 tacgttgagc tgaacctgat ttctttaaat gcatttgacc tgaccctgac tctgcctggt
     1861 atcgctggtg ttatcctgtc gatcggtatg gcagtcgatg cgaacgttat catctacgcc
     1921 cgtatcgagg aggagatcgg tgccggaaag acggttcatg cagcaatccg cgacggattc
     1981 aagaaggcag cttccgccat cctggacgga aacgtaacga ctctgattgc agcagccgtt
     2041 ctgtatgcac tcgcaagcgg aagtgtccgc ggattcgcca tgacacttgc tcttggtatt
     2101 gttctttcca tgttcagcgc catggttgtt tccagactgc tggtcaacag cctgtacggt
     2161 atgggactga aggatgcgaa atactacgga accaagaaag agcgcaaggg atttccattt
     2221 gtggaaaaga gaaagatctt cttcacgatc tcctgcattc tgctgcttgc cgttccggcc
     2281 agcatgattt tcatgcatca gacaaaggga agcgcactga acttcggcct tgacttcaag
     2341 ggcggtactt ccatcaacgt tccgttcaac gaggattact ccatcgaaga gctggacaaa
     2401 gaggtagagc cggttgtgga aggcgtgaca aaagattcca atattcagat gacaaaggtt
     2461 gtcggaggca acaatgtcat catcaaaaca cgttctctga ctctggaaga gcgtgagcag
     2521 gtttaccagg caatggcaga caatttcggt gtcgatactt ctgaaatcac tttcgacaac
     2581 atttcttcta ctgtaagtaa agagatgagc cagaatgcaa tgaaagcagt tatcatcgct
     2641 gtagtctgca tgctgctgta catctggatc cgtttccgcg acatccgttt tgcatcgagt
     2701 gccatccttg cactgatgca cgatatcgcc attgtattcg gattctatgt ggtatcccgt
     2761 atttcggtcg gcagcacctt tatcgcgtgt atgctgacga tcctcggcta ttccatcaac
     2821 agtacgatcg tcatcttcga ccgtatccgt gagaatctgc cggagttaaa gagagagccg
     2881 atcgagtccc tggttgaccg ctgcatcacg gacaccctga cccgaagcat ttacagttcc
     2941 ctcaccacct ttatcaccat ctttgttctg ttcgtgatgg gagtttcttc catccgcgac
     3001 tttgcagcac ctctgatggt tggtatcgta tgcggagctt acacttctgt ctgcatcaca
     3061 ggtgccctgt ggtatgtgat gaagcacgtt ggaaaaaaca aatatgtgcc gtccaaagca
     3121 gtacagaata agacaaaaaa taaataaaag cagcaaaaac cataaaaaga gaaacgccgg
     3181 accggaaaag tccggcgttt cctgctaaca ggaaagagga gaatatatga agaaagaacg
     3241 ctgggtagtt tcggcaaaac gcgccgattt ccaggcaatt gcggaccggt tcggcatcga
     3301 tcccgtcatt gcccgcctga tccggaaccg ggatgtgacc ggggagaaag agatcgaaga
     3361 gtatctcttc ggtgatctgg agcagctcca cgatccgctg ctgatgaagg atatggagcg
     3421 cgcggcagat ctgatcgcag aaaaaatccg ggagaaaaag ccgatccgca tcatcgggga
     3481 ctacgacatc gacggtgtta cggcaacgta tattctgctg accggcttaa aagatctcgg
     3541 tgccgatgtg gatgtgcgta tcccggaccg gattgcagac ggatacggct taaatgagca
     3601 tcttgtgcag tttgccgcgg atgaggggcg ggacacgatc gtcacctgtg acaacggcat
     3661 cgcggcgggc agtcagatcc gtctggcaaa agagctcggc atgacggttg ttgtgacgga
     3721 ccaccacgag gttccgtttg aggaggatga aaacggagaa cgccgctaca tcctgccgcc
     3781 ggcggacgcg gttgtcaatc cgaaacagaa agactgttcc tatccgtttc cgggcctctg
     3841 cggtgcggcc gtcgcatgga aactgatcca gacgatggag gcaaaagcag gtattccaaa
     3901 ggagcacagc ttccgctttc tggaatttgt cgccattgcg acgatcggtg atgtgatgga
     3961 tctgcaggga gaaaaccgga tttttgtaaa agccggcctt cgggcgctcc ataaaacgca
     4021 gaacctcggc ctgcaggagc tgatccgtgt gcagggcatc gaggcggaaa acgtgacacc
     4081 gtatcacatc ggcttcgttc tcggcccgtg catcaacgca tcgggacggc ttgacacggc
     4141 ggagcattcg ttaaaactgc tctgcgcaaa gacacgtgag gaggccgcaa agcttgccgg
     4201 ggacttgaaa gacctgaatg aaagccgcaa agagctgacg cgccagggtg aggcgaaagc
     4261 aatcgagctc gtggaaacct ccccgctgca gaacgacaaa gtcctcgtcg tctatctgcc
     4321 ggactgccac gagagccttg ccggaatcat cgcaggtcgc ctgcgggagc acttccacaa
     4381 accatccttc gtactcacca gaagtgagga gggcgtcaag ggctccggcc gctcgatcga
     4441 agcgtattcg atgtatgagg agctctgcaa atgcaaagaa ctgatgacga aattcggcgg
     4501 ccacccgatg gctgcgggcc tttccctcgc aggagaggag atgatcgaac cgttccgccg
     4561 ggcactgaac gataattcca cgctgacgga agaggatttc gtcgaaaaag tcgtgatcga
     4621 tgtgccgatg ccgatcacct acatcaccaa aaatctgatc cgccagttaa gtctcttgga
     4681 gccgttcgga aaaggcaaca cgaaaccgct gttcgcacag aaaggactga ctgtattaaa
     4741 ttaccgcatt tttggaaaga atagaaacgt ggtgaaagta cagcttgccg atgcgggcgg
     4801 ataccagatg gatggtgtct atttcggcga gggagaagcg ttcgccgcat acgtggatgc
     4861 acatccggcc ctctcggtag cgtactatcc gtcgatcgat acgtggaacg gacgcgacaa
     4921 attacagatc aatatccaga gttattttta aagatgcaga aaagccgcaa atacctgatt
     4981 tttctgccct gttttctatc ggaaacgaaa ttgacgcaga agaatattga tgctatactg
     5041 taaggtgaat tgtcaaacct tgacgaaacg gaggaatatt ttatgaaaaa agtagaggat
     5101 tacgtaagaa gtattccgga tttcccggaa ccgggtatta ttttcagaga tgtaacgagt
     5161 atcctgcagg acgcagacgg actgcagctt gccatagatg agatgcagca ttttgtagaa
     5221 gaagtagact gtgatgtaat ctgcggaacc gagtcccgcg gcttcatttt cggtatgccg
     5281 attgcttaca atctgcacaa accgttcgtg ccgatccgta aaaaaggaaa acttcctctg
     5341 gagaccgtcg aggagagcta tgacctggag tacggcagcg caacgatcga gatgcacaaa
     5401 gactccatca aaccgggcca gaaggtagtc atcatcgacg acctgattgc aaccggcggt
     5461 accgtggagg cctgtgcaaa aatgatcgag cgcctcggcg gcgaagtaac cagaatcgtg
     5521 ttcctgatgg agcttgccgg cttaaaagga cgcgaaaaac tggcaaaata tgatgtaaaa
     5581 tctgtcatcc gttacgatgg caaataacgt atccaacgaa acgcaggggt taatcatgag
     5641 agcggaggat acatatggaa gaagagaaaa gaatcagtga agaagaatta aagaaggatg
     5701 tggcggcggc agaagccaga atccgcaaga cggacgattt tacaagtccg gaggaattat
     5761 acggggaatt gatcgcttct gtgaaaaaat atcatccatc ggacgatatt tccctgatcg
     5821 aaaaagcata ccgcgtggca gcggatgcac ataaggacca gaaaagaaag agcggggaac
     5881 cgtacatcat ccatccgctc tgtgtggcaa ttatcctggc agatctggag ctcgacaagg
     5941 agtcgatcgc ggcaggcctt ctgcacgacg ttgtcgaaga taccgtcatg accgaagaag
     6001 agctgaccga ggagttcggc ccggaggtgt ccctgattgt aagcggtgtc acgaagctgg
     6061 cacagctgaa atattctgcg gataaggtcg aggagcaggc agaaaacctg agaaagatgt
     6121 tccttgcgat ggcaaaggat atccgcgtta ttctggtaaa actggcggac cgtctgcaca
     6181 acatgcggac cgcacagtac tggtcgccgg agacgcagaa gaaaaaagcg cgtgagacga
     6241 tggatatcta tgccccgatc gcacagcgcc tcggtatttc caagatcaag gtcgaactcg
     6301 atgacctttc tctgaaatat ctggagccgg atgcgtacta tgaactggtg cagaaggtca
     6361 gcatgaagcg ggagcagcgg caggagtttg tcgatgcgat cgtaaaagag gtgacccgtc
     6421 atatccacca ggccggcatt gaggcagatg tcaacggacg aatcaagcac tttttcagca
     6481 tctacaaaaa gatggtcaac cagcacaaga cgctggatca gatctatgac ctctttgcgg
     6541 tccgcatcat cgtggaatcc gtcaaagact gctatgcagc gctcggtgtc atccatgaaa
     6601 tgtacacgcc gatcccgggc cgtttcaaag attacattgc gatgccgaag ccaaaccgtt
     6661 accagtccct gcatacaacc ctgatcggac cgaacggaca gccgtttgag attcagatcc
     6721 gtacttatga gatgcaccgg attgcagaat acggtatcgc tgcacactgg aaatacaaag
     6781 aggcctccga cggcaagaaa ccgggaccgg agcaggaaga ggaaaaactg acctggctgc
     6841 gccagattct ggagtggcag caggacatgg cggacaacca tgagttcatg aacctcttaa
     6901 agagcgatct cgacctgttt gccgacaatg tttactgttt cagcccggcg ggcgatgtca
     6961 aaacgcttcc ggtcggctcc tgtgccatcg actttgctta ctccgtccac agtgcggtcg
     7021 gcaacaagat ggtcggagcg agggtcaacg gaaaactcgt gccgatcgag acggaacttc
     7081 acaacggaga ccgcgtcgag atcatcacct cgcagaactc caagggaccg agccgcgact
     7141 ggctgaaaat tgcgaagagt tcccaggcaa agagccgcat caaccagtgg ttccgccagc
     7201 agaacaagac ggacaacatc gtcaaaggca aggaaatgct caccaactat gcaaagctga
     7261 agggcaaaaa ccttggaatc tatacgaaac cgatgtacga agaggctgtc atgagaaagt
     7321 acggcttccg cgactgggat tccgttcttg cggccatcgg ccacggcggc ttaaaagagg
     7381 gccaggtcct caacaagctg attgagacgt acgaaaagca gcataaaaaa gagatcaccg
     7441 acgagcaggt gctcgaggcg gcatccggag agaaagttct gccggttgga aagagcaagg
     7501 gcggcatcgt cgtgcgcggt atccacgatg tggcagttcg cttctccaaa tgctgcaatc
     7561 cgatcccggg cgatgagatc gtgggctttg tcacaagagg acgcggcgtg accattcacc
     7621 gcaccgactg tgtcaatgtc ctgaatctgc cggaatctga gcgggcgcgc ctcattgatg
     7681 ctgaatggca ggcagaggac aacggcggct ctctctatac cgtggagatc agcgtttacg
     7741 caaacaaccg taccggtctt ctggtggata tctccaagat ctttaccgag cggaagatcg
     7801 accttgcggc gatgaacgtg agaacgagca agcagggaac ggcttccatc gatgtgacgt
     7861 tcgacgtgca caacaacgaa gagctgaaca gcatcatcga gaaggtgcgc caggtggaga
     7921 gcgtgatcga tattcagaga tcgagaggat aaaaaggaga aaagcgtatg ataaaggtaa
     7981 attaccgggc acttggaatg ctctcgacaa actgctacgt tgtggaaaat ccggacagcc
     8041 agaaggtgct gatcgtggac ccgggcgatt cgccgctgga gattgaacgc cagatcgatg
     8101 agatcgacgg cgacccggag gccattctgc tgacccatgg ccatttcgac catatgctgg
     8161 cagcggatgc gctgcggaaa aaataccata tcccggtcta tgtccatgaa aaagagcagc
     8221 acctgctggg ggatccaaag gaaaatcttt ccgggctctg gagcaaaccg tacacgatgc
     8281 aggcggacaa aaccgtaaaa gagggcgatg tgctgcatct cgcagatttt gaaatccacg
     8341 ttcttgcgac accggggcac acggcaggcg gtgtctgcta ctatattccg gcagagaaag
     8401 cacttttttc cggtgatacg ctgttctgcg agtcctacgg aagatacgac ttcccgacat
     8461 cctccggcag agagctgatc gcgtcggtca agcgcctgtt aaaagagctt cccggggatg
     8521 tgaccgtgta tccgggccac aacgatgaaa ctacgattga acatgaaaag aggtacaatc
     8581 cccttgctta atatcagcct ttgttttaac agaaaagatt ttgaatacga tgtgtattcc
     8641 ctgatcaagg cattctatcc gggctgtgag attacctcat ggtacgaaga ggatggggcg
     8701 ctggacgggg aatttgcata ttatgatacg atcacatacg aggcggatca gatctgtttt
     8761 tcgattgcag acgagaaaca tgagacgctc gcttcgcagt gtgaggcggt ggaatatgaa
     8821 aaagaccgcc atgaaacgaa aaatgtcctg aaacggatgg tataccgcac gctttccgaa
     8881 gtgagcggca aagaacttcc atggggcgat ctcaccggca tccgtccgac gaaaattccg
     8941 atgaagatgc ttgaagaggg taaaaaaaat gtagagattg cgaaatacat gcgcgaaact
     9001 tactacacca gcccggaaaa gacggcactt gcgatcacga ttgccaaccg ggaaaaggac
     9061 attttaaaga cgatcgacta cgaacacggt tacagcctgt acatcggcat cccgttctgt
     9121 ccgagcatct gcctgtactg ttccttcggc tcccatgtgt taagccgctg ggagcatatg
     9181 gtagatccgt atctcgatgc actgatcaaa gaactgatct ttatcagtga gaacatgaag
     9241 gattacaccc ttgatacgat ctacatcgga ggcggtacgc cgaccacgct gaatgcggca
     9301 cagatggagc gcctgctcac gaaagtgaca gagctcttcc cgatggagca ggtgcaggag
     9361 ttcaccgtgg aggcaggacg gccggatacg atcaatgagg aggtcttaaa ggccatccgc
     9421 aaattcccgg tcacccgtat ttcgatcaat ccacagacca tgaaccagga aacgctggat
     9481 ctgatcggac gccaccatac cgtggaggag atcgaggaga agttccgcat ggcgcgttcc
     9541 cttggatttg acaacatcaa catggatctg atcgtcggtc tgccgggcga ggacaaggaa
     9601 aaagtggcgc atacgttgga aaaagtggaa gcgctgaatc cggattccct gaccgtccat
     9661 tcccttgcgt taaagcgtgc aacgagactc aacctgttca aagacaaata tcaggagatc
     9721 tcgtttgaaa actctgccga gatcatgaaa atgacgatgg acagcgcaca ccgcatggag
     9781 atgggaccgt attacatgta ccgccagaaa aatatggcgg gtaactttga gaatgtcggt
     9841 tattcccggg aagggaaagc aggcatctac aacatcctca ttatggagga aaaacagtcc
     9901 attctggccg cgggagccgg agcgtccacg aaattcgtgt ttgaacacgg agagcgcatt
     9961 gagcgggtgg agaacgtgaa ggatctgaaa aactacgtgg agcgtatcga tgaaatgatc
    10021 gagagaaagc gtatcggaat ggagaagtat ctgccaaaat gaaagaaaat tccagtcagc
    10081 agcttcacat gctgcgctat accctgaccg agcagctcat ccatggcatg gaagtgagca
    10141 atttagctta cgatcttgcg cgggaactgg ggtatgagaa agaaatctgc tatgagcttg
    10201 caaaagcggg cgttctccac gatatcggaa agattgtact ggagaattac gtagaagaac
    10261 aggatacgct cgtcgtcgag gagatgcgct ttgtgcggac ccattccaca ctgggctatg
    10321 aactcctgca gggacgcggc tactccgatt ttgtgctgga gagtattctg tatcaccatg
    10381 aaaattatga cggaaccggg tatccggcca atctggcggg agaaaagatt ccgtttgggg
    10441 cgcgtatctt acgcatctgt gatgtgtact gcgcgctgac ctccgaccgc ccgtaccgga
    10501 gtgcgtttcc gcgggaacag gcgatggagc ttatgacaga agaagtcaaa aatttcgatc
    10561 tgaaaatgtt cctggcattt cagagagtga tccacagcgg cagccggaaa gcgatcgagc
    10621 tttctgatgt cgatgaattg atccgggaaa tcataaagga gaaaacagaa aatggcatta
    10681 aagaagaaac cggttaccgg aatgaaagat attttaccga aagaaatggc aatccgtgac
    10741 tacgtgatcc gtctgatcaa ggaaacttac ggaaccttcg gctttacctc catggagacc
    10801 ccatgcgtgg agcacatcga aaacctgaac agcaaacagg gcggcgagaa tgagaaactg
    10861 attttcaaga tcttaaaaag aggagagaag ctgaaactcg agaccgctga gaaagaaaac
    10921 gatctcgtag acagcggcct gcgttatgac ctgaccgttc cgctctgccg ttactattcc
    10981 aacaacgcaa acgagcttcc gtccccgttc aaagcacttc agatgggaaa tgtatggcgt
    11041 gcagaccgtc cgcagagagg aagataccgc cagttcatgc agtgcgatat cgatatcctc
    11101 ggcgagccga ccaacctcgc agagattgag ctgatccttg ctaccaccac actgcttgga
    11161 aaactggact tcaagaactt tacgatccgt atcaatgacc gccgcatctt aaaagcaatg
    11221 gcagcctaca gcggattccc ggaggagtcc tatgacaccg tatttattat cctcgataag
    11281 atggataaaa tcggttttga gggcgttgcc aaagagctcg aggaagccgg ctttgcaaaa
    11341 gagtccgtag agaaatacct gaaaatgttc gaggagatca ccccggatac cgcaggcgta
    11401 gagtattgcc gtgagaaact cgagggattc cttgacaaag agtacgcaga cggcctgaag
    11461 accatcatcg acagtgtcaa tgccgtgaag accgcagaat tcaagatcgc tttcgatccg
    11521 actctggtgc gcggaatgtc ctactacacc ggaccgatct tcgagatcgc tatggatgag
    11581 tacggcggat ctgtcggagg cggcggacgc tacgatgaaa tgatcggaaa attcaccgga
    11641 aaccagacct gcgcatgcgg attctccatc ggatttgagc gtatcgttat gcttctgctt
    11701 gagagagatt accagatccc gggcgcacag aagaaaaaag cgtacctgat cgagaaaaac
    11761 atgccggcag agggaatgca gaagatcatt cagcaggcga caaaagagag aagtgaaggc
    11821 acggccatca acatcgccgt gatgaagaag aacaagaaat tccagaaaga gcagcttgcc
    11881 agcgagggtt acactgagat cgaagaattt tttgttgaca aaatgtaagg aatatccaat
    11941 aatagtaatc gcatgaaaaa acgatgtgct gcggatgcag attctttgtg tccgcagttc
    12001 caaataggag gaacaagaaa atggctgaat ccatgaaagg cctgaagaga acctgccgct
    12061 gtgcggaagt cacaaaagca gatatcggaa aaaaagtaac cctgatgggc tgggtacaga
    12121 agagccgtaa taaaggcgga attattttcg tcgatctgcg tgacagaagc ggtattatgc
    12181 agattatctt tgagaacggc ccgatcagtg aggaaggttt tgagaaagca gcaaaactgc
    12241 gcagcgagtt cgttatcgcc gtagaaggtg aagttatggc ccgctccggc gcggtcaatg
    12301 aaaatctggc aacaggagag ctcgaagtaa aggctgagtc cttacgcatt ctttccgagg
    12361 cagaaacgcc gccgttcccg attgaggagg attccaagac gagagaagag ctccgtctga
    12421 aataccgtta cctggatctg cgccgcccgg acatccagag aaacctgatc atgagaagca
    12481 aagtggcaat gctcacccgt gagttcatgt ccaaagaagg cttccttgag atcgaaaccc
    12541 cgatgctgac gaaatccaca ccggaaggcg ccagagatta cctggtaccg agccgtgttc
    12601 atccaggcag cttctacgca ctgccgcagt ctccgcagct gttcaaacag cttctgatgt
    12661 gctccggcta tgaccgttat atccagatcg taaaatgttt ccgtgacgag gacctgcgtg
    12721 ccgaccgtca gccggagttc acccagatcg atatggagct tgctttcgtt gatgtagatg
    12781 atgttatcga tgtcaatgag agactgctgg cacacctgtt caaagaggtg ctcggcgttg
    12841 aggtacagct cccgatccaa agaatgacct ggaaagaggc tatgaaccgt ttcggttccg
    12901 acaaaccgga cctgcgtttc ggtatggagc tggttgatgt ttccgagacc gtaaaagata
    12961 ccgagttcgt tgtatttaag ggcgcacttg aaaacggcgg ttctgtccgc ggtattaacg
    13021 caaagggaca gggcgcaatg ccgagaaaga aaatcgacaa gctcgttgag tttgcgaaag
    13081 gttacggtgc aaaaggtctt gcatacattg caatccatga ggatggaagc tggaaatctt
    13141 ccttctccaa atttatgaca gaggagcaga tggaagccct catcaaagcc atggacggac
    13201 aggctggaga ccttcttctc ttcgcagcag accgcaacaa gattgtttgg aacgttcttg
    13261 gcgcacttcg tctggaactg gcagagcaga tgggactgct cgacaagaac gagtaccgtt
    13321 tcgtatggat taccgagttc ccgcttctgg agtggtccga tgaggagaac cgcttcactg
    13381 caatgcatca cccgttcacc atgccgatgg acgaggatct gccgcttctg gacaccgatc
    13441 cgggcgcagt tcgtgcaaaa gcttacgata tcgtcctgaa cggcactgag atcggaggag
    13501 gaagcgttcg tattcaccag aacgatgtgc aggagaagat gtttgagatg cttggtttca
    13561 caaaggaaca ggcacatgag cgatttggtt tccttctgga tgctttcaaa tacggagttc
    13621 cgcctcacgc cggactggct tacggactgg accgtctcgt tatgctgatg gcaaaagagg
    13681 acagcatccg cgacgtcatc gcattcccga aggtaaaaga tgcttcctgt ctgatgaccg
    13741 aggctccgtc accggttgac gcagcacagc tggaagagct tggtcttgag gttgagccgc
    13801 aggctgagga agagacaaca gaagagtaaa gaaaattttg tgatttacag gacagacaat
    13861 tttgcagaag tgcagcgttg tctgtccttt tttaggtttc actcgatcgg tgtaaagaaa
    13921 aacttaagaa attcttccac gtcttccccg ttgttttgct ggaaaaaagt gctacaatat
    13981 ggtttgtaag aaagatacgg aaacatccac agcaagtgga acacatagag aaaaagacaa
    14041 ggagggatca cgatgttagt tacattagtc atgctggcaa cctgttttgg tctgctctgc
    14101 ggaattggaa ttttatttgg gatcgtcgga ctgctgctga aatttggagg aaaagtcctc
    14161 ggcgcatgcg ccactattct tctcccgatc ggcgtgatct ggcttggatt taaaatcgtg
    14221 ttcgccgtcg ttccgcttct gttcaaactg gtctgtgcag cgctgatctg ttatctcgta
    14281 taccgcattc tgcgggagat gaaacttgta gcataaaaaa taaaatctaa cagattcgaa
    14341 agaaaacctg ctttccgtca taaacacgga ggcaggtttt taatgacaaa aacaaaaatc
    14401 tattttcaga aaaagccagt atttgctaaa aaaagagtaa ggaaaaagta agaaaacaaa
    14461 aaacaaaatg tgatacgata gaactaactt gggaaagcag aaaattaatt tgtaaaatag
    14521 aaaagtggga ggaagcatat gaagaaaaaa gtaatcgcga ttgtgtgtgc agtcgcagtg
    14581 ctggcggcag cc
//
LOCUS       DAOMUF010000034        14409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_904_length_14409_cov_4.301937, whole genome shotgun sequence.
ACCESSION   DAOMUF010000034 DAOMUF010000000
VERSION     DAOMUF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14409
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_14409_cov_4.301937"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLN77_03500"
     CDS             <1..911
                     /locus_tag="PLN77_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM43381.1"
                     /translation="TQKLYKYDRECLQEYMDLVQQVTDYSNQLADEKADLEASEAEQE
                     AQQQSLEEMLEQKKAVSDDYENQIADLNDMAAQYNAVLAEQNAKIQQIQEEIAAEEAA
                     RKAEEARQAAEAAEAARQAEAARQAAAEAAASSAANNTTTADNTPSSTGSSTTTTTPS
                     NGGSSYDDSSSSDTSSSDDSSSSSSTGSATGSAIVSYATQFIGNPYVWGGTSLTNGAD
                     CSGFIMSVYAHFGYDLPHSSSALRGVGRGVSYAEAQPGDIICYSGHVAIYIGGGAIVH
                     ASNERDGIKISSNAAYTTILAVRRVV"
     gene            1152..1853
                     /locus_tag="PLN77_03505"
     CDS             1152..1853
                     /locus_tag="PLN77_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43382.1"
                     /translation="MRKVASVLSICIIAGLLIVYVPNIIAGAGKIGDTVNTMKTADLP
                     FGKALYAAFLYGTFQLANVAVFVQHAKSFEKPQDAGKSMAVGAVLNALLMIMVVLGIM
                     TVYQNPEMIQQSVPTLFMVQQGVGSKFMTPLISVLIILGAVSTAVNMVAAMVKRIHAG
                     LAERSSRTETAGKISRTQILAALVCCIADFLIAQFGLLTLIQKVYSILAYLAIPVILV
                     PYVVHMAVMRFDTKK"
     gene            2148..2867
                     /locus_tag="PLN77_03510"
     CDS             2148..2867
                     /locus_tag="PLN77_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM43383.1"
                     /translation="MKNAPAYVKVYSRLKNEINKGTYPVGGFLPKECELEKIYQVSRT
                     TVRNAVKMLVQEGLVEVRQGSGTRVLNHKAIQNYNKVTSVTESLMKKGYRVTTSDMMI
                     DIISADKQLADELEIPEGTSVARIQRLQLADEEPVTLMENYIEYAKVPGIEAYENQFV
                     ALYQFLEEKFGLQIDETRDRISAKSATFMEAQVLKTEPKDALLVVHRVTYWQNRPVSI
                     DHVRIIGSKYEVEISGKGRSK"
     gene            2864..3550
                     /gene="deoC"
                     /locus_tag="PLN77_03515"
     CDS             2864..3550
                     /gene="deoC"
                     /locus_tag="PLN77_03515"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRM43384.1"
                     /translation="MSDETEEQKKLMRQIDISCVQAFHTMDEIDAMVELAKKYRLAAV
                     FTLPAFSAHVAERLQDTREIHAGGVVSFPGGGDTTGQKKRQAKELWEAGCREIDMVMN
                     LTAFRSHEFSYVKEELLAIREQVPSAIFKVIIEAPQLTEQEICQAVDLCVEGRADYVK
                     TSTGWYPGCPSTVEQVRIMSREAAGRIQIKAAGGIRSLETIQEMQKAGCSRFGMGMRS
                     VQNLIKSIYK"
     gene            3694..4755
                     /locus_tag="PLN77_03520"
     CDS             3694..4755
                     /locus_tag="PLN77_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRM43385.1"
                     /translation="MKGYAMLKIGESGWIEKERPKCGPMDAICRPLAVAICTSDVHTL
                     WEGAIGDRHNMILGHECCAEVVEVGELVKDFKPGDRVLVPAITPDWNSLEAQAGYSMH
                     SGGMLAGWKFSNFKDGVFSEFFHVNDADGNLALLPKNINVVDACMLSDMVPTGFHGVE
                     LADVQFGDTVLVIGIGPVGLMSVAGTNMRGASRIIAVGTRPVCVEAAKKYGATDFVSY
                     KNGPIFQQVLDMTDGKGVDKVVIAGGGVETFAEAVKCLKPGGKIGNVNYLGSGDNIDI
                     PRVEWGVGMGHKQINGGLMPGGRLRMEKLGALVSAGKLDVSPLSTHVFDGWDHLEEAL
                     FLMRDKPKDLIKPVVKLAD"
     gene            4788..5732
                     /locus_tag="PLN77_03525"
     CDS             4788..5732
                     /locus_tag="PLN77_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM43386.1"
                     /translation="MKILIVSGFLGAGKTTFIKRLARNVDQRFAILENEYGAAGIDGA
                     RLGQNRQGTQKNVWEMTEQCICCIGKKDFAASVLTIANVVDPEYLIVEPTGVGSLARI
                     IENLRQIEYERIQLLSPVTIVDVYSYRRYMAEYPELYQDQIRSADTIIVSKTEACQAE
                     EKQRIQKFLQEINPQAKIITDYASTMTREDYQSLLLGGRYEKEVQDTQSGEKMPETFS
                     FENIRMEAPETLWCFLEQIIRGAFGNIIRAKGQFAAGDQFLQFDVADHRYSITGTEQK
                     NAGQAVFIGNDMNEDAIRQYFKKCAGNQKMKYAARHVS"
     gene            5905..6987
                     /locus_tag="PLN77_03530"
     CDS             5905..6987
                     /locus_tag="PLN77_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43387.1"
                     /translation="MEEQKRRRERKSAAYWLSKSSILYPICILYVMYVKQVEVYITEC
                     SVHISSGTMLFAYALCLILWLASPIRSYCADGTANEVLFYAFPPSILMLFLFAQYSLK
                     IVLFLAVLDGIFLVTVLRDLHRKKRKYSPKKFWRLKRSDLNAFLFVSVCVSMVPTCMM
                     IYWKLESPSGVAELYERKKDYNLVQNQENIFQENKEFLLSLKESEWRQKTLEERTIAA
                     QKMVRLETERLGIPEIPLYVKETGSSNCVALYNNEENEIWYAPEHLTSQTAEEFFTGI
                     CEECYHGMEYYLLERMDWNSEMANTAYFEEMRKWKLNDNRYISGRDEGDSFEAYQSQP
                     LEASAKKYASSETEALIHFIAVYGDE"
     gene            7174..9780
                     /gene="clpB"
                     /locus_tag="PLN77_03535"
     CDS             7174..9780
                     /gene="clpB"
                     /locus_tag="PLN77_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRM43388.1"
                     /translation="MNISKFTQKSLQAVQDLEKTAYDFGNQEIEQEHLLYNLLHQDDS
                     LILKMIEKMEINKDHFLNRVETALNDRVKVSGGQPYIGQYLNKALVNAEDEAKAMGDE
                     YVSVEHLFLAMLKNPSPSMKKLFQEYGITRERFLQALSTVRGNQRVTTDNPEATYDTL
                     EKYGQDIVEKARNQKMDPVIGRDTEIRNVVRILSRKTKNNPVLIGEPGVGKTAVVEGL
                     AQRIANGDVPESLKDKTIFSLDMGALVAGAKYRGEFEERLKAVLEEVRKSDGKIILFI
                     DELHLIVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLDEYRQYIEKDAALERRFQP
                     VQVDEPTVEDTISILRGLKERYEVYHGVKITDGALVAAATLSNRYISDRFLPDKAIDL
                     VDEACALIKTELDSMPAELDEQRRKILQMQIEEAALKKETDNLSRERLETLQKELAEL
                     KDTFNSAKAQWENEKSSVEKLSKLREQIEDMNRQIQKAKNDYDLNRAAELQYGELPKL
                     QQMLEAEEKKVKNEDLSLVHESVTDEEIARIVSRWTGIPVAKLTEGERTKILGLEDEL
                     HTRVIGQNEAVTKVSDAIIRSKAGIKDPTKPIGSFLFLGPTGVGKTELAKTLAEKLFD
                     DENNMVRIDMSEYMEKYSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVLFDEIEKA
                     HPDVFNVLLQVLDDGRITDSQGRTVDFKNTILIMTSNIGSQYLLDGIQDDGSISEEAR
                     NLVMQDLRAHFRPEFLNRLDETIMFKPLTKDNIGHIVDLLLKGLNKRLADQELTVELS
                     PAAKQFVIEGGYDPVYGARPLKRFVQKEVETSTAKLILGGQVSEGDTILLDVENGGLK
                     AMIKPGVEVVDE"
     gene            9777..9950
                     /locus_tag="PLN77_03540"
     CDS             9777..9950
                     /locus_tag="PLN77_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HRM43389.1"
                     /translation="MMPKDPVMLLSYVNTQLRDFYPSLAEFCAAMQTEESDLREKLAL
                     IDYEYDEKTNQFV"
     gene            10157..11797
                     /locus_tag="PLN77_03545"
     CDS             10157..11797
                     /locus_tag="PLN77_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRM43390.1"
                     /translation="MYFGVQMYGVSKEWKQDPEGFLKKIYEAGYRQIEPCLGFRVDAR
                     DYGFWIPEDLEQAMPLLAKYHIEVHAVHIFLDEYHYERELAILTELAQKYHISWFVVK
                     SPARLTKDVLDETAARYRELAEELEKAGAGLLIHNEKEDICIRVNGKTAYEYLLEACG
                     EKVGAEVDAGWMYCGGVDPEEFLWAHADRVKAVHYKDMKITGQEAPLGKGMVDLKACF
                     QFARANGALQIVDMDAATLEDTCRAGKMLSGWTGDRDNTDSILCTMDVETGEETVLHE
                     FPGIIEAPNWLNDGNTLLYNADGKIYRYEIDKDHVEQVDTGFCVQCNNDHVPSPDNQL
                     LAVSCMPPELTDGTYESHIYVLPMTGGEPKDLTGPGLSYLHGWSPDGKELAYCAFRKK
                     PEEETMRIEICTIPSDGGEETCLTDGKGYNDGPEYSPDGKHIWFNSTRSGLMQVWRMN
                     RDGSGLTQMTDSDANNWFGHVSPDGKHVIYLTFAKGELEPNEHLPNMYVSLGMMDYDG
                     QNKKKLLDLFGGQGSINVNSWAPDSRRIAYVKYVLHHK"
     gene            12010..14286
                     /locus_tag="PLN77_03550"
     CDS             12010..14286
                     /locus_tag="PLN77_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43391.1"
                     /translation="MKKTKKMLSFVLSMLLIVTMFPTMAFAKEEAKTWKFGELSLKAG
                     DVLGKDTEIKNDAENREIRILSEKTNPDEKKDQKDDKERIKTEKEAVIAAAASWNLKD
                     LTEKAKKAPADYLLKKADSWNGSWIVTKIAETETKDAIEIQIRTYEYAAVTEIQGIPK
                     EIPGTTALTGKAVPENADQKQITWEITDAGMTGAVLDGTNLKVTNAGTVKLLATIKDG
                     KKTGVDFTQEFTVIVKAADYTKVTEALALIPEDMGRYTEESAAAVQKAKDAVKENLPS
                     AEQETVNGYAAAIQTAVNALTLLGADYTEVDAVLAKVPGDLSIYTEESVEALNAVIAS
                     IDRTKTIEEQQAVAAYAEALENAIAALVRKPVPADYQGVEELLGKIPKDLSIYTEKSV
                     KALNAAKEAIVWDLDDSRQEEVDQSAENLKAALDGLTLKPADYSAVDAALAKVSNDLS
                     IYTEESIQPLQTAINSVESGKTILDQAEVDGWAAAIENALAGLQVRKADYSKVEEAIK
                     KIPADLSLYTDASVKALEDAKNSVVTERPVTEQESVDGYAKKIEAAIAGLMYKDADYS
                     KVDAAVKKIPNDLKKYTDESVKAVNDAKAAIVRGKNITEQKTVDGYAAALEKAIAGLK
                     QKPMTAQNLPKITKGVNQSGMQGKELSFTADFEAKDLKKVLIDQKEIDAKNYVTAGKE
                     NTQVTLKAEYTKSLAEGKHTISIVSSKGQADTVFYLKKATKSPDTGDRTQVMLWVILL
                     GVSAAAVVGAVVYRKREK"
BASE COUNT         4417 a         3129 c         3871 g         2992 t
ORIGIN      
        1 acacccagaa attatacaaa tacgacagag agtgcctgca ggagtacatg gatctggtac
       61 agcaggtaac ggattacagc aatcagctcg cagacgagaa agcagacctg gaagccagcg
      121 aggcagagca ggaagcacag cagcagtccc tcgaggaaat gctcgagcag aagaaagccg
      181 tttccgatga ctacgagaac cagattgcag acctgaatga catggctgca cagtacaacg
      241 cagtactggc agagcagaac gcaaagatcc agcagatcca ggaggagatt gcagccgaag
      301 aggcagcaag aaaagccgaa gaggcccgtc aggcagcaga agcagccgag gcagcccgcc
      361 aggcagaagc agcaagacag gcagcagcag aagcagccgc aagcagtgct gcaaataata
      421 caacaacggc agacaacaca ccgtccagca caggaagctc caccacgaca acaacaccgt
      481 ctaacggagg aagctcttac gatgattctt cgtccagcga taccagcagt tcggatgatt
      541 ccagcagctc cagcagcacc ggaagcgcaa ccggaagtgc catcgtatcc tatgcaacac
      601 agtttattgg aaatccatac gtatggggcg gcacaagcct taccaacgga gctgactgct
      661 ctggttttat catgagcgtt tacgcacact tcggatacga ccttccgcat tcctccagtg
      721 cactgcgtgg tgtaggacgc ggtgtatctt atgcagaggc tcagccgggc gatattatct
      781 gctacagcgg ccacgtagcc atctacatcg gcggcggtgc aatcgtacac gcaagcaatg
      841 agcgcgacgg tatcaagatc agcagcaacg cagcgtatac cacgattctt gcggtaagac
      901 gtgtggttta aaaaagaatt acatagtaag aattttcaga tcccgggaga gaaaaatccc
      961 gggatctttt gcgttttgct taaaattgag tgcattcgcg ttaaaactgt gatatactaa
     1021 agcacatcgg agatacaagg gcgtattgac caacagaagg tcagtatgcc cttttgcttg
     1081 ttaggaagaa gggagaatcg ttatgaaact ggaaaattat tttcgtcgtt tcggtattcg
     1141 gaacggatct ggtgcggaaa gtagcgtcag tgctgtccat ctgcattatt gccggtctgc
     1201 tgatcgtcta cgtcccgaac atcatcgccg gagccggaaa gatcggcgat actgtaaaca
     1261 ccatgaaaac cgcagacctc ccattcggca aagccctgta cgccgcattc ctctacggaa
     1321 ccttccagct tgccaatgtc gcagtcttcg tccagcatgc caaatccttc gaaaaaccgc
     1381 aggatgccgg aaagagcatg gcagtcggtg ccgtattaaa tgcccttctg atgatcatgg
     1441 tcgtcctcgg aatcatgacc gtataccaga acccggaaat gatccagcag agcgtcccga
     1501 cattatttat ggttcagcag ggcgtcggca gtaaattcat gacaccgctg atttccgtac
     1561 tgatcatcct cggtgccgta tccacagccg tcaacatggt tgccgccatg gtcaaacgaa
     1621 tccacgccgg tctcgctgaa cgaagcagca gaacagaaac cgcaggcaaa atcagccgca
     1681 cccagatcct tgccgcgctt gtctgctgca tcgcggactt ccttatcgca cagtttggtc
     1741 tgctgacgct gatccagaag gtgtacagca tcctggcata cctggcaatc ccggtcatct
     1801 tggtgccgta cgtggtgcac atggcggtga tgcggtttga tacgaaaaaa tagaaaagaa
     1861 caataaaaaa tttgtaacaa aaatttacac cgcagaaaga gagatttgct tctggagaat
     1921 ggaagtattt cgcagacagc agaaaaaaga agattcatgc agagcagatg tgagtcataa
     1981 agaaatgaca tgcgtctgcc ttttttctgt gcgtctggca gagtatgtgt cggtgacaaa
     2041 gcagaaaaca ggaactcttc tttgggactc gattgtgccg tcaaaatatg gtatactgca
     2101 aaaaggatca aaaagcggat attgatattt cacgggaggg gaagaagatg aaaaatgcac
     2161 cggcttatgt gaaggtttac agcagactga aaaacgagat caataaggga acatatcctg
     2221 tcgggggatt tctgccgaag gagtgtgaac tggagaaaat ctatcaggtc agccgtacta
     2281 cggtacggaa tgcagtaaag atgctggtgc aggagggact ggttgaggtt cgccagggtt
     2341 ctggaacgag ggtgctgaat cataaggcaa ttcagaatta taacaaggtc acatcggtca
     2401 cggaatccct gatgaaaaaa ggatatcggg taacgacgtc agatatgatg atcgacatca
     2461 tttcggcgga caaacagctc gccgatgaac tggaaattcc ggagggaacc agcgtggcga
     2521 ggattcagcg gctgcagctt gcggatgaag agcctgttac gctgatggaa aattatatcg
     2581 aatatgcgaa agtccctggc atcgaggcgt atgagaatca gtttgtagcg ctgtatcagt
     2641 ttctggagga gaaattcggc ttgcagattg atgagacacg agacagaatt tcagcgaaat
     2701 ccgcgacctt tatggaagcc caggttctga aaacggagcc gaaggatgcg cttcttgtgg
     2761 tgcacagagt cacctactgg cagaaccggc cggtgagcat tgaccacgtg cggatcatcg
     2821 gaagcaagta cgaggtggag atttcgggaa aaggacggag taaatgagcg atgagacaga
     2881 agaacagaag aaactgatgc ggcagattga tatcagctgt gtacaggcat ttcatacaat
     2941 ggatgaaatt gatgcgatgg tggagctggc aaagaaatat cgtctggctg ctgtgtttac
     3001 cttgccggcg ttcagcgccc atgtggcaga acggctgcag gacaccagag aaatccatgc
     3061 gggaggcgtg gtaagctttc cgggcggcgg agatacgacc ggacagaaga agcggcaggc
     3121 aaaagaactg tgggaggctg gctgccggga aatcgacatg gtgatgaatc ttacggcatt
     3181 cagaagccac gaattttctt atgtaaaaga agaacttctg gccatccggg agcaggtgcc
     3241 ttcggcgatt tttaaggtga tcatcgaggc gccgcagctg acggagcagg aaatctgcca
     3301 ggcagtggat ctgtgtgtgg aaggccgtgc ggattatgta aagacgagca ccggatggta
     3361 tcccggctgt ccttccaccg tggagcaggt tcgaatcatg agccgtgaag ccgccggtcg
     3421 cattcagatc aaggccgccg gtggaatccg cagtctggaa actatacagg aaatgcagaa
     3481 agccggctgt agccgtttcg gtatgggaat gcggtcggta cagaatttga taaaaagtat
     3541 ctataagtaa tctattgtgc aaaagctata aaaaagtcac ggaatattct acggcattcc
     3601 gtgacttttc tgttgaatta gtaacctatt agtgatatat tcgaaccata ggaatcgata
     3661 aatcattaac aatctgaaag gagagcaaaa gatatgaaag gttatgcaat gctgaaaatc
     3721 ggtgaatccg gatggatcga gaaagaacgc ccgaaatgcg ggccgatgga tgccatttgc
     3781 cgtccactgg cagttgccat ctgcacatca gatgtccaca ctttatggga aggggccatc
     3841 ggtgaccgtc ataacatgat tctgggccat gagtgctgtg cagaggttgt ggaagtcgga
     3901 gagctcgtaa aagatttcaa accgggagac cgggttcttg tgccggccat cactccggac
     3961 tggaattctc tggaagcaca ggctggttat tccatgcact ccggcggaat gctggcaggc
     4021 tggaaattct ccaacttcaa agatggtgtc ttttctgaat tctttcatgt aaatgatgcc
     4081 gatggaaacc ttgcgctgct tccgaaaaac atcaatgttg tcgacgcgtg catgctttcc
     4141 gatatggtgc cgaccggatt ccacggcgta gagcttgccg atgtacagtt tggtgataca
     4201 gttcttgtca tcggaatcgg accagttggt ctgatgtcgg tagccggaac gaatatgcgc
     4261 ggcgcatcga gaatcattgc tgtcggaacg agaccggtct gcgtagaagc ggcaaaaaaa
     4321 tacggcgcaa cggattttgt cagctacaag aacggaccga ttttccagca ggtactcgat
     4381 atgacagatg gaaaaggtgt tgataaagta gtcatcgccg gcggtggtgt agaaaccttt
     4441 gcagaagcag taaaatgcct gaaaccgggc ggaaagatcg gaaatgtaaa ctatcttgga
     4501 tccggtgaca acattgatat tccgcgtgta gagtggggcg ttggaatggg tcacaaacag
     4561 attaacggag gcctgatgcc tggcggaaga ctccgcatgg aaaaactggg agctcttgtt
     4621 tcggcaggca agctggatgt cagcccgctg tccacgcatg tgtttgatgg atgggatcat
     4681 ctggaagaag cactgttcct catgagagat aaaccgaagg atttaatcaa accagtcgtt
     4741 aaattggctg actgatgatt tgaaaatttg actgaaaaag gaaacgtatg aaaatattga
     4801 tcgtatccgg ctttttgggc gcgggaaaaa caacatttat caaacggctg gccaggaacg
     4861 tcgatcagcg gtttgccata ctggaaaatg aatatggagc cgcaggaatc gacggcgcac
     4921 gtcttggcca gaaccggcag ggaacgcaga aaaatgtatg ggaaatgaca gagcagtgca
     4981 tctgctgcat cgggaaaaag gattttgcag catccgtgct gaccattgcc aatgtggtag
     5041 acccggaata cctgatcgta gaaccgacag gcgtcggaag tctcgcccgg attatagaaa
     5101 atctgcgtca gatcgagtac gagagaattc agcttctctc gccggtcact attgtggatg
     5161 tttacagtta ccgccgctat atggcggaat atcccgagct gtatcaggat cagatccgaa
     5221 gcgcggatac gatcattgtt tccaaaacag aagcatgcca ggcggaagaa aaacagcgca
     5281 tccagaaatt tctgcaggaa attaacccgc aggcaaaaat catcacggat tatgcatcca
     5341 cgatgacacg ggaagattac cagagcctgc tgctgggcgg ccgatatgag aaagaagtac
     5401 aggatacgca gagcggcgaa aagatgccgg agaccttctc cttcgaaaat atccgaatgg
     5461 aggcaccgga aaccctgtgg tgttttctgg aacagatcat tcgcggagcg tttggaaata
     5521 tcatccgtgc aaaaggacag ttcgcagccg gagaccagtt tctccaattt gatgtcgccg
     5581 atcaccgcta cagcatcacc ggcacagaac agaaaaacgc cggccaggct gtgtttatcg
     5641 gaaacgatat gaacgaagat gcgatccggc agtattttaa gaaatgcgcc ggaaaccaga
     5701 aaatgaaata tgctgcccgt catgtatcat aaagtaaata ggaaaaagag attgcggagt
     5761 cctgccaaaa cagggtttcg caattttggt ttttacagaa agattcaact gaaaaacgaa
     5821 ggataggcaa aaacgaaaaa ttacgctaaa ataaacatac aagtattcca aataaaacaa
     5881 aagaaaaatc aggggggatc tgatatggag gaacagaaaa gaagacggga aagaaaatca
     5941 gcggcatact ggttgagcaa aagctcgatt ttatatccga tctgtatctt gtatgtgatg
     6001 tatgtgaaac aggttgaggt atatattaca gaatgttccg tccatatttc gtcggggaca
     6061 atgctttttg cctatgcgct gtgcctgatt ttgtggctgg cctcacccat tcgaagctac
     6121 tgcgcagatg gaactgcaaa tgaagtttta ttctatgcat ttccgccgag catactgatg
     6181 ctgtttttat ttgcccaata ttcactgaaa attgtgttgt ttcttgcagt gctggatggg
     6241 atatttcttg ttacggtttt aagagattta catagaaaaa aacgaaaata ttctccgaaa
     6301 aaattctgga gattaaaaag aagtgactta aacgcatttc tgtttgtttc agtatgcgtg
     6361 agtatggtac cgacttgtat gatgatatat tggaagcttg agtctccgtc cggagtggca
     6421 gaactttacg aacgaaaaaa ggactataat ctcgtccaga atcaggagaa catttttcag
     6481 gaaaataaag aatttctgct gagcttaaag gagagcgaat ggaggcaaaa aacactggaa
     6541 gaaagaacaa ttgcagcaca gaagatggta cggcttgaaa cagaacgact tggaattccc
     6601 gaaattccgc tgtatgtgaa agagacagga tcatcgaatt gcgttgcatt atataataac
     6661 gaagagaacg aaatatggta tgctccagag catttaacat ctcagacagc agaagaattt
     6721 tttacgggaa tttgcgaaga atgttatcat ggaatggagt attatctgct ggagcgaatg
     6781 gactggaatt cggaaatggc gaatactgct tattttgaag aaatgaggaa gtggaaacta
     6841 aatgataatc gctacatatc tggacgcgac gagggcgatt cgtttgaagc atatcaatca
     6901 cagccgctgg aagcatctgc caaaaagtat gccagttccg aaacagaggc actgattcat
     6961 tttattgctg tgtacggcga tgaataaaaa tcttttaggg ggggagggag aaaaggagcg
     7021 tcgggaaaaa ataaaaaaat tagcactcac ctcttgacag tgctaataac tagtgttata
     7081 gtacggatag aacaaagaaa agaaaattca catcaacgct ctttttgttc ggagcgaatg
     7141 ttagcaccca aatagataag gaggaagccg attatgaata tcagtaaatt cacacaaaaa
     7201 tcgctgcagg ctgtacagga cctggaaaag accgcatacg atttcggcaa ccaggaaata
     7261 gagcaggagc atctgttgta taatctcctg catcaggacg acagcctgat tctgaaaatg
     7321 atcgaaaaga tggaaatcaa caaagatcat tttctgaacc gtgtcgagac ggcgctgaac
     7381 gaccgcgtca aagtatccgg cggtcagccg tatatcggtc agtatctgaa taaagcactg
     7441 gtaaatgctg aggatgaggc gaaagccatg ggagacgagt atgtttccgt agaacacctc
     7501 ttcctggcaa tgctgaaaaa cccaagcccg tccatgaaaa aactgtttca ggagtacggc
     7561 atcacaagag aacgtttcct gcaggcatta agcaccgtgc gcggaaacca gagagttacc
     7621 accgacaacc cggaggcaac atacgacacc ctggaaaaat atggccagga tatcgtagaa
     7681 aaagccagaa accagaaaat ggacccggtt atcggccgtg ataccgaaat tcggaacgtt
     7741 gtccgtatcc tgtcccgtaa gacaaaaaac aatccggttc tgatcggtga gcctggtgtt
     7801 ggtaaaaccg cagtcgtaga aggcctggcc cagcgaatcg ccaacggcga tgtaccggag
     7861 agcctgaaag ataaaaccat tttctccctc gatatgggag ccctggtagc cggagcaaaa
     7921 taccgcggcg aattcgagga gcgtctgaaa gccgttctgg aggaagtcag aaagagcgac
     7981 ggaaaaatca ttctgttcat cgatgagctg cacctgatcg tcggcgccgg aaagaccgac
     8041 ggcgcgatgg atgccggaaa catgctgaaa cctatgctgg cacgaggcga actgcactgc
     8101 atcggtgcca caacgctgga tgagtaccgc cagtacatcg aaaaagatgc cgccctggag
     8161 cgccgtttcc agccggttca ggttgatgag ccgaccgtag aggatacgat ttccatcctg
     8221 cgtggcttaa aagagcgcta cgaagtttac cacggcgtaa aaatcaccga cggtgccctc
     8281 gttgcagccg caaccctttc gaaccgttac atcagcgacc gtttcctgcc ggataaagca
     8341 atcgacctgg tcgacgaggc atgcgctctg atcaagacag aactggattc catgccggcc
     8401 gagctggatg agcagcgccg taagattctg cagatgcaga tcgaggaagc cgcactgaaa
     8461 aaagagaccg acaatctgag ccgcgagcgt ctggaaacac tgcagaaaga gctggctgag
     8521 ttaaaggata ccttcaattc tgcaaaggca cagtgggaaa atgaaaagag ttccgttgag
     8581 aaactgtcca aactgcgtga gcagatcgag gacatgaacc gccagatcca gaaagcaaag
     8641 aatgattacg atctgaaccg cgccgctgaa ctgcagtacg gtgaactccc gaaattacag
     8701 cagatgctgg aagcagaaga gaaaaaagtt aaaaatgaag atctctccct ggtacacgag
     8761 agtgtaacgg atgaagagat tgccagaatt gtctcccgct ggactggaat cccggttgca
     8821 aaactgaccg aaggcgagag aactaagatc ctcggcctgg aagatgaact tcacacgaga
     8881 gttatcggcc agaatgaggc agtcaccaag gtttcggatg caatcatccg ttccaaagcc
     8941 ggaatcaagg atccgacaaa accgatcggt tcgttcctgt tccttggacc taccggtgtc
     9001 ggtaaaacag agcttgcgaa aacgttggca gaaaagctgt ttgatgatga gaacaacatg
     9061 gttcgaatcg atatgagtga atacatggag aaatactccg tctcccgtct gatcggagcg
     9121 cctccgggat atgtaggcta cgaggagggc ggccagctga ccgaggcagt gcgcagaaaa
     9181 ccatattctg ttgttctgtt cgatgagatc gagaaagcgc atccggacgt cttcaacgtt
     9241 ctgctgcagg tactcgatga cggccgtatc accgactccc agggccgtac cgtagacttt
     9301 aaaaatacga tcctgatcat gacctccaat atcggatccc agtatctgct ggacggcatt
     9361 caggacgacg gcagcatctc cgaagaggcg agaaacctgg tcatgcagga tctgcgtgca
     9421 cacttccgtc cggagttcct gaaccgtctg gatgaaacaa ttatgttcaa accattgaca
     9481 aaagacaaca ttggtcatat cgtagatctg ttgctgaaag gactgaacaa acgtctggca
     9541 gaccaggagc ttaccgtcga actgagcccg gctgcaaaac agtttgtcat cgagggcggc
     9601 tacgaccctg tttacggcgc ccgtccattg aaacgtttcg tacagaaaga ggtagaaacc
     9661 agcaccgcaa aactgatcct tggcggtcag gtcagcgagg gcgatacgat ccttctggat
     9721 gtggaaaacg gcggcctgaa ggcaatgatc aaaccaggtg tggaggtagt agacgaatga
     9781 tgccgaaaga tcccgtaatg ctgctgagct atgtcaatac gcagttaaga gatttttacc
     9841 cgtcgctcgc agaattctgc gcggccatgc agacagagga atccgatctg cgggaaaaac
     9901 tggcgctcat cgactatgag tacgatgaga aaacgaacca gtttgtataa gaataaccag
     9961 gccctgaaaa aaatggcgga aaaaggcgtt ccgtatgtgg aaaaaacaca tacggggcgc
    10021 cttttttacg caaagcgtgt ttcttataac tgacaggaaa aatcatatct ataatagaag
    10081 aaatgtggta aaatggagga aaagaaaatc cgctgaaaat ggggaaacag cggatctgga
    10141 gaaggaggaa acaaatatgt attttggtgt acagatgtat ggggtatcca aagaatggaa
    10201 acaggatccc gaaggatttt taaagaaaat ctatgaggca ggctaccgcc agatcgagcc
    10261 gtgtctcgga ttccgtgttg atgccaggga ctacggtttc tggatcccgg aggatctgga
    10321 gcaggcaatg ccgctgcttg caaaatacca cattgaggtt catgcggtcc acattttcct
    10381 ggatgaatac cactatgaaa gagaacttgc cattcttaca gaactggcgc agaaatacca
    10441 catttcctgg tttgtcgtaa agagcccggc acgcctgaca aaagatgttc tggacgaaac
    10501 cgctgcaaga taccgcgaac tggcagaaga actggaaaaa gccggagcag gtcttctgat
    10561 ccacaacgaa aaagaagata tctgtatccg cgtgaatgga aaaacagcgt acgaatatct
    10621 cctggaagcc tgcggtgaaa aagtcggagc agaagtagat gccggctgga tgtactgcgg
    10681 cggcgtcgac ccggaagagt tcctctgggc gcatgcggac cgagtcaaag ccgttcatta
    10741 taaagatatg aaaatcaccg gccaggaagc gccgcttgga aaaggcatgg ttgatctgaa
    10801 agcctgcttc cagttcgccc gcgcaaacgg tgcactgcag attgtggata tggatgcggc
    10861 aacactggaa gatacctgcc gcgccggaaa aatgctctcc ggatggacag gggacagaga
    10921 caacaccgac agcattctct gcacgatgga tgtggagacc ggagaagaaa ccgtgctgca
    10981 tgagtttccg ggaatcatcg aggcaccgaa ctggctgaac gatggaaaca ccctgcttta
    11041 taatgcggat ggaaaaatct accgctatga gatcgacaaa gaccatgtgg agcaggttga
    11101 taccggtttc tgcgtgcagt gcaacaatga ccacgttccg tcaccggaca accagctgct
    11161 ggcggtaagc tgcatgccgc cggaactcac agatggaacg tatgaatccc acatctacgt
    11221 gctcccgatg acaggcggtg aaccaaaaga cctcaccgga ccgggtctca gctatctgca
    11281 cggctggtcg ccggacggaa aagagcttgc gtactgtgcc ttccgcaaaa agccggagga
    11341 ggagactatg cgcattgaga tctgtacgat tccatcagac ggcggcgagg aaacctgcct
    11401 gacggacggc aaaggctaca acgacggtcc ggagtattca ccggacggaa agcacatctg
    11461 gttcaacagc accagaagcg gtctgatgca ggtatggcgg atgaaccgtg acggcagcgg
    11521 tctcacccag atgaccgatt ccgatgccaa caactggttc ggccacgttt cgccggatgg
    11581 aaaacacgtc atttacctga ccttcgctaa aggtgagctg gaaccgaatg agcatctgcc
    11641 gaacatgtat gtatcgcttg gaatgatgga ttatgacgga cagaataaga aaaaactgct
    11701 ggatcttttc ggcggccagg gcagtatcaa cgtcaactcc tgggcaccgg acagccgtag
    11761 aattgcgtat gtgaaatatg tgcttcatca caaataaagg aatatcttaa gaaaatctga
    11821 cgggccgtaa aaattcatgg aagtattgtg aaaaaacgtt gaaagtattt tagcggaaat
    11881 gcacaagaat tcttacgaat ttttacggcc cgttgactct ttttctgaca cacgtataat
    11941 gaaagtaaca agagaaactg cggcccctgg cggctgcaaa acaatcaaaa ggaaaggagg
    12001 atttgtgtta tgaagaaaac gaaaaaaatg ctttcgtttg tactgtccat gcttctgatc
    12061 gtgacgatgt tcccaaccat ggcattcgca aaagaggaag ccaaaacgtg gaagttcgga
    12121 gagctttcgc tgaaagcagg cgacgttctc ggcaaagata cggagattaa aaatgatgca
    12181 gagaacagag aaatccgaat actttcagaa aaaacaaatc cggatgaaaa gaaagatcag
    12241 aaagatgaca aagaaagaat aaaaacagaa aaagaggctg tgatcgcagc ggcggcttcc
    12301 tggaatctga aggatcttac ggaaaaggcc aaaaaggctc cggcagatta tctgcttaaa
    12361 aaggcagaca gctggaacgg aagctggatt gtaacaaaga tcgcagaaac ggaaacaaaa
    12421 gatgccattg aaatccagat aagaacctat gagtacgcag ctgtaacaga aatccagggc
    12481 ataccgaaag aaatcccggg aaccaccgca ctgacgggca aagcagttcc agaaaatgca
    12541 gatcagaaac agatcacatg ggaaatcacg gatgccggaa tgacaggtgc tgttttagac
    12601 ggcacaaacc tgaaagtaac gaatgccgga accgtaaaac ttctggcgac aatcaaagac
    12661 ggaaagaaaa ccggcgttga cttcacacag gaattcactg tgattgtaaa agccgcagat
    12721 tacaccaaag taacagaagc gctggcgctg atcccggagg acatgggccg gtatacagaa
    12781 gaaagcgcgg cagctgttca gaaagcaaaa gatgcggtaa aagagaacct gccatcagct
    12841 gaacaggaaa ccgtaaatgg ttatgcggca gccattcaga cagcagtaaa cgcactgacg
    12901 cttcttggcg cagattacac agaagttgat gcagtacttg ccaaagttcc gggagatctt
    12961 tctatctata cggaagagag cgtggaagca ctgaatgcag tcatcgcatc catcgaccgc
    13021 accaaaacga tagaagagca gcaggcagtt gctgcttatg cggaagcact ggagaatgca
    13081 attgcagcac tggtacgcaa accggttccg gcagattatc agggagtgga agaactcctg
    13141 ggaaagattc cgaaagactt atccatttat accgaaaaga gtgtaaaagc actgaatgcg
    13201 gcaaaagaag ccattgtatg ggatctggat gacagcagac aggaggaagt cgatcagtct
    13261 gcagaaaacc tgaaagcagc tcttgacgga ctgaccctga aaccggcgga ctacagcgcc
    13321 gtagacgcgg ctcttgccaa agtttcgaat gatctgagta tttatacgga agaaagcatt
    13381 cagccgcttc agacagcgat caacagcgta gaaagtggaa aaaccattct ggatcaggca
    13441 gaagtagacg gctgggcagc agcaattgaa aatgcactgg caggactgca ggttcggaaa
    13501 gcagattatt ccaaagtaga ggaagccatc aaaaagattc cggcggatct cagtctgtat
    13561 accgatgcgt ctgtaaaagc actggaagat gcaaagaaca gcgtcgtaac cgagcggccg
    13621 gtcacagagc aggagagcgt agacggttac gcaaaaaaga tcgaggcagc gattgccggc
    13681 cttatgtata aagatgcgga ctacagcaaa gtagatgccg cagtgaagaa gattccaaac
    13741 gatctgaaga aatacacgga cgagagcgta aaagcagtca atgatgcaaa agcagcgatt
    13801 gtacgcggaa agaatatcac ggagcagaag acggtggatg gttatgcggc agcgctggaa
    13861 aaagcgattg ccggcctgaa acagaaaccg atgactgcgc agaatcttcc gaagattacc
    13921 aaaggagtca atcagtcagg tatgcagggc aaggagcttt ccttcaccgc agattttgaa
    13981 gcaaaggatc tcaaaaaggt tctcatcgac cagaaagaaa ttgatgcgaa gaattatgtg
    14041 acggctggaa aagagaatac gcaggttacg ctgaaggcag agtatacaaa gagtctggca
    14101 gaaggcaagc atacgatttc gatcgtttca agcaagggcc aggcggatac cgtattctat
    14161 ctgaagaaag caacaaagag tccggacacg ggagaccgca cacaggtgat gctgtgggtg
    14221 atcctgctcg gcgtttctgc agcagcagtg gtcggagcag ttgtttatcg taaacgtgag
    14281 aaataagagt tgaaagggaa agggcgccgg aaggcgcctt ttcttttgga cggtttcgga
    14341 tagattgtaa atagtcagat aaaatacaaa atatgcagaa ttaactatat ttttctaaaa
    14401 ataagaaaa
//
LOCUS       DAOMUF010000035        14036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_948_length_14036_cov_2.875617, whole genome shotgun sequence.
ACCESSION   DAOMUF010000035 DAOMUF010000000
VERSION     DAOMUF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_948_length_14036_cov_2.875617"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>504
                     /locus_tag="PLN77_03555"
     CDS             112..>504
                     /locus_tag="PLN77_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM43392.1"
                     /translation="MKQYKGAIFDLDGTLLDSMRVWDDIDVAFLKKRGLEVPPDYQEA
                     ITPLGFLEAARYTIRRFGFPETSEELIQEWHQMAVDAYTYEVELKDGAAEYLRYLKEK
                     GIRMAVATSSSPELYEPALKRNGIYEYFK"
     assembly_gap    505..532
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <533..794
                     /locus_tag="PLN77_03560"
     CDS             <533..794
                     /locus_tag="PLN77_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM43393.1"
                     /translation="TVSEVKRGKGFPDIYEKAAEKLSLPPETCVVYEDILAGIRGAKM
                     GGFAAVGVYDRSGEGNRAKMEQEADRYVTSFKELMNGGDSFF"
     gene            916..2841
                     /locus_tag="PLN77_03565"
     CDS             916..2841
                     /locus_tag="PLN77_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRM43394.1"
                     /translation="MPKKETYDASSISVLEGLEAVRKRPGMYIGSTSRKGLNHLIYEI
                     VDNAVDEHLAGYCDTIHVTLEADGSATVTDNGRGIPVGMHEKGVPAERLVFTTLHAGG
                     KFDNNAYKTSGGLHGVGSSVVNALSTYLDVQVSLGGWVYHDRYERGVAVQELDEDGLL
                     PKIGKTKNSGTRINFLPDPEIFEKTRFSATEVKSRLHETAYLNPALTIYFSDLRDGKE
                     EHLTYHEPDGIVGFVRDLNKNKEAIHDPIYFKGESEGITVECAFQYINEFQENVLGFC
                     NNIYNAEGGTHISGFKSTFTTIMNSYAREIGVLKEKDNNFTGSDIRNGMTAVISIKHP
                     DPRFEGQTKTKLDNPDAAKAAGKVTGEEIPLYFDRNLEVLKNVLACAERSAKIRRSEE
                     KAKTNMLTKQKYSFDTNGKLANCEKKDPSLCEIFIVEGDSAGGSAKTARNRNFQAILP
                     IRGKILNVEKASIDKVLANAEIKSMINAFGCGFSEGYGNDFDITKLRYDKIIIMADAD
                     VDGAHISTLLLTLFYRFMPELIYEGHVYIAMPPLYKAMPSRGKEEYLYDDKALEKYRK
                     THKEKFTLQRYKGLGEMDPEQLWETTLNPETRLLKRVEIEDARMASDVTEVLMGTEVP
                     PRKAFIYEHATDAELDI"
     gene            2860..5115
                     /locus_tag="PLN77_03570"
     CDS             2860..5115
                     /locus_tag="PLN77_03570"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HRM43395.1"
                     /translation="MKEKSEDQIIRTEYSEVMQKSYIDYAMSVIISRALPDVRDGLKP
                     VQRRTLYDMYELGIQYDKPFRKCARIVGDTMGKYHPHGDSSIYDALVVMAQDFKKGQP
                     LVDGHGNFGSIEGDGAAAMRYTEARLQKITQEVYLKDLDKDVVDFVPNFDETEKEPSV
                     LPVRVPNLLLNGADGIAVGMATSIPPHNLCEIVDAVKAYMKNNDITVKGLMKYIKGPD
                     FPTGGIVVNQDDLLSIYEKGVGKIKIRGKVEVEKGRNGKQYLVITEIPYPMIGANIGK
                     FLNDVAGLVESKKAPEIVDISNQSSKEGTRIVLELKKGADVERITNLLYKKTRLEDTF
                     GVNMLAVADGRPETLSLKQVIEHHVDFQFEVATRKYQNLLRKEMERREVQEGLIKACD
                     VIDLIIEILRGSKTQKQVKECLIMGVTDGIRFKSKSSERMAAQLCFTERQATAILEMR
                     LQKLIGLEVEALLKDHEATVAKITKYEDILNHYDSMSEVIMEDLTQIKKEYGHKRKTA
                     IENAEAIVLEEPKVEEMDVVFLMDRFGYAKTIDTSAYERNKEAVHNENKYVFQCKNTD
                     KICVFTDLGMMHSIKVMDIPYGRFRDKGTPIDNLGNYSSQKEQMIYVDALENIKNNKL
                     LFVSAAGMVKLVDGSEFDVVKRTIAATKLSSEEDRLILAEVCQEQEYVVLQTKNGVFL
                     RFLTSEVPEKKKAAVGVRGIRMAEDDAVEHAYLLASRMDYTITYHDKEISLTAKIKLA
                     KRDTKGTKIRV"
     gene            complement(5241..6641)
                     /locus_tag="PLN77_03575"
     CDS             complement(5241..6641)
                     /locus_tag="PLN77_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRM43396.1"
                     /translation="MTMQYDNLEDKLSQLVEYTKDSGKIDLSLYTEYDVKRGLRDSTG
                     KGVLTGLTEISDVIGFDIDERTGEKVPTDGRLYFQGYNVADLIKGMEGRRFGFEEITY
                     LLLFGSLPTEPQLNDFNEILSIYRELPDTFVRDVVMKATSKNMMNTLQRCVLTLYSYD
                     EKPDDISIPNVLRQALSLIAKMPLIAVYGYHAYRHYHENQNLIIRNPKPELSIAENIL
                     QMLHPDGDYTALEAKVLDVALILHADHGGGNNSTFTTHVVSSSGTDTYSAVAASLSSL
                     KGPRHGGANLKVMEMFENIEENVTNWEDENELRTYLTKILDREAFDGTGLIYGMGHAV
                     YTLSDPRCVILKEYAKKLSAEKHREKEFARYESVERIAGECIAKKRRLLKPVCANVDF
                     YSGFVYTMLGLPKELYTPIFAISRISGWSAHRIEELVNEGKIIRPAYKYVGHHRPYVA
                     IERRTPRKTSHSFSNN"
     assembly_gap    7010..7019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7211..7858)
                     /locus_tag="PLN77_03580"
     CDS             complement(7211..7858)
                     /locus_tag="PLN77_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRM43397.1"
                     /translation="MANKRNYQKELDAKIAGLHGEVPTLLLHSCCAPCSSYVLEYLSQ
                     YFSITLFYYNPNIYPPTEYEHRVEEQQRLIHSLPAVHPISFCPGPYDSDRFYKLTRGM
                     ENVPEGGERCFVCYEMRLREAAEMAKQLKLDYFTTTLSISPLKNAEKLNEIGERLAAE
                     YGIPYLNSDFKKRGGYQRSIELSHEYGLYRQDYCGCVFSRRERDARVAAQENGNS"
     gene            7969..8739
                     /locus_tag="PLN77_03585"
     CDS             7969..8739
                     /locus_tag="PLN77_03585"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43398.1"
                     /translation="MDDQILSLEHISLSYHTMKGETPALCDLTFSVTPGEFVALVGPS
                     GCGKSTILNLISGLLKPEAGTLLSRGRPITEADLHIGYMLQRDHLFEWRTIYSNVLLG
                     LEISRTLTPERKEKVGEMMETYGLKSFSNARPSELSGGMRQRAALIRTLAMEPDLLLL
                     DEPFSALDYQTRLNVCDDIGKIIKKEGKTAILVTHDISEAISMADRVIVLAARPAYVK
                     KKIPIQFAMEERTPMKARSAPEFKTYFNAIWKELNENA"
     gene            8732..9577
                     /locus_tag="PLN77_03590"
     CDS             8732..9577
                     /locus_tag="PLN77_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43399.1"
                     /translation="MREQAKTPESSFQRAYLRQKKREREQVRVFRALLLVSFLVLWEV
                     AADLRWIDPFIFSSPVRVGKTFVTLLTEQNLLRHIGITVYETGISFLLVSTLGMAFAV
                     LLWLFPRFSAVMEPYLVVLNSLPKSALAPLLIVWMGANVKTIITAGISVAIFGSVISI
                     YSSFQSVEPDMLRLIATLGGTKKDMLFRVVLPWSFPGLLAVWKVNIGLCLVGVVIGEF
                     IGAREGLGYLIIYGSQVFRYAHAREGRMTDDWNRMSGRTKRNAVWRTTRFAGPRGNGK
                     NLRTV"
     gene            9594..9890
                     /locus_tag="PLN77_03595"
     CDS             9594..9890
                     /locus_tag="PLN77_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRM43400.1"
                     /translation="MNPASEKLFAEQKESGKVTLQAAADFLEQAGEGEYCFVENTGLQ
                     AVEAKIEKIIVFWWNRHYPSDRKFDLDLSKWNKVSEEEFAGYSHEKITKEVYEK"
     gene            10019..10825
                     /locus_tag="PLN77_03600"
     CDS             10019..10825
                     /locus_tag="PLN77_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRM43401.1"
                     /translation="MTESQITDQAPTAVSVLDVPEFSGEPYVVLNGNEPDFTDEEKTT
                     ESYEHYSDLDSLGRCGVAEANIGQDLMPTEKRGAIGQVKPTGWHTVKYDQVEGKYLYN
                     RCHLIGYQLTGENANEKNLITGTRYLNVEGMLPFENMVADYVKETGNHVLYRVTPIFT
                     GDDLVADGVEMEALSMEDDGEGLSFHIFAYNNQPGISINYATGDSTLSESSGTMTDQQ
                     EYVMNTSSMKFHLPSCSSVSSIKDENKATYQGPREDLIAEGYEPCGRCNP"
     gene            10898..11791
                     /locus_tag="PLN77_03605"
     CDS             10898..11791
                     /locus_tag="PLN77_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM43402.1"
                     /translation="MRQEMEEEKLRAVYYARCSTEEENQKDALVRQAAEAEEAIRARG
                     WRLVDAYVESRSGTSRKGRREYNRLYEDLEQNRFDVVVIKSQDRLMRNTRDWYLFADR
                     LNCAGKRLYFYLENTFYSPEDALLTGIKAILAEEYSRELSKKMNLAHQKRQQNNGKPV
                     LTSKSYGYRRTADGAIEIIPEEAAVKRRMYELCADGLGGRKIAAVLAAEGVRSRAGKP
                     FSGTDIIRMVRNPMNKGTVVMNKKHYDFNTGKTLPTPPGGQYVYAGKIPAIVSEELWI
                     AANRAADERLCRKKVGKSVQT"
     gene            11985..13364
                     /gene="argH"
                     /locus_tag="PLN77_03610"
     CDS             11985..13364
                     /gene="argH"
                     /locus_tag="PLN77_03610"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRM43403.1"
                     /translation="MAQLWGGRFTKETDKLVYNFNASISFDQKFYKQDIKGSMAHVKM
                     LAKQGILTEEERDQILAGLEGILADVESGKLEITSEYEDIHSFVEANLIDRIGDAGKK
                     LHTGRSRNDQVALDMKLYVRDEITEIDALVKEVLVTLEKIMEENIHTYMPGFTHLQKA
                     QPITLAHHMGAYFEMFRRDRGRLADIYRRMNYCPLGSGALAGTTYPLDRAYTAELLGF
                     DGPTLNSMDSVSDRDYVIELLSALSTIMMHLSRFSEEIIIWNTNEYQFVDIDDAYSTG
                     SSIMPQKKNPDIAELVRGKTGRVYAALMSMLTTMKGIPLAYNKDMQEDKELTFDAIDT
                     VKGCLALFNGMLSTMHFKKDRMENSAKNGFTNATDAADYLVGKGVPFRDAHGIVGRLV
                     LYCIDKGISLDDMKLEEYKAISPVFEDDIYDAISMKNCVERRNTIGAPGADAMLAVIE
                     QNKKYLQEN"
     gene            13452..>14036
                     /locus_tag="PLN77_03615"
     CDS             13452..>14036
                     /locus_tag="PLN77_03615"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRM43404.1"
                     /translation="MEQKLRVGILGATGMVGQRFISLLENHPWFEVVTLAASPRSAGK
                     TYEEAVGGRWKMDTPMPEAVKKLVVMNVNDVEHVASTVDFVFSAVDMSKDEIKAIEEA
                     YAKTETPVVSNNSAHRWTPDVPMVVPEINPEHFDVIEFQKKRLGTKRGFIAVKPNCSI
                     QSYAPVLTAWKEFEPYEVVATTYQAISGAGKTFKD"
BASE COUNT         3889 a         3104 c         3882 g         3123 t
ORIGIN      
        1 acttgcattt tttgccagtt atggtatgat aaaacaaaca aatgttctaa aaaaacagaa
       61 atacgttcta aaaagaaaga aaaaagatta gaatagagaa ggagaagaca aatgaaacag
      121 tacaaaggag ccatttttga tctggacgga acactgctcg actcgatgcg ggtctgggat
      181 gacatcgatg ttgcattttt aaagaagaga ggacttgagg ttccgccgga ttatcaggag
      241 gcgattacac cgctcggctt tctggaggct gcccgctaca cgatcagacg gttcggcttt
      301 ccggaaacgt cggaagagct gattcaggag tggcatcaga tggcggtcga cgcctatacg
      361 tatgaagtgg agttaaaaga cggggcagca gaatatctcc gctatttaaa agaaaaagga
      421 atccgcatgg cggttgctac ctcatcgagc ccggagctgt atgagccggc cttaaagaga
      481 aatgggattt atgagtattt taaannnnnn nnnnnnnnnn nnnnnnnnnn nncaccgtct
      541 cggaagtaaa gcgcggcaag ggctttccgg atatctacga gaaagcggct gaaaagctgt
      601 ctcttccgcc ggaaacgtgt gtggtatatg aagatatcct agcgggaatc cgcggcgcca
      661 aaatgggcgg atttgcggct gtgggcgtgt atgacagaag cggggaagga aaccgcgcaa
      721 agatggagca ggaagcagac cgctacgtga cttcttttaa ggagctgatg aacggaggag
      781 attctttctt ctaaagaaaa tcttgctatt ccgggaagaa aaatgatata ataaaatact
      841 gtgccccaaa atgggacaga cagaagtatt tttaagagaa aaagacagta acacataagt
      901 gaggatagaa cagttatgcc aaaaaaagaa acgtatgatg ccagcagtat ttccgtgctg
      961 gaaggtctcg aagcagtgcg taagcgccct ggtatgtata tcggaagtac ctcgcggaaa
     1021 ggcctgaatc atctgattta tgaaattgta gacaatgcgg tggacgagca tcttgcgggc
     1081 tactgcgaca ccattcacgt aacacttgaa gcagacggct cggcgaccgt gaccgacaac
     1141 ggccgcggaa tcccggtcgg catgcatgaa aaaggcgttc cggcggagcg tctcgttttt
     1201 acaaccctgc acgccggcgg taaattcgac aacaatgcgt acaagacaag cggcggtctg
     1261 cacggtgtcg gttcttcggt tgtcaatgcg ctttccacct atcttgacgt acaggtcagc
     1321 ctcggcggct gggtttacca cgaccgctac gaacgaggtg ttgccgtaca ggaactcgat
     1381 gaggatgggc tgctgccgaa gatcggaaaa acgaagaaca gcggaacccg catcaacttc
     1441 ctgccggatc cggaaatctt tgagaaaaca cgtttttccg caacagaggt caaaagccgt
     1501 ctccatgaga ctgcatatct gaacccggcg cttaccattt atttttccga tctgcgcgat
     1561 ggaaaagagg agcacctgac gtatcacgag ccggacggca ttgttggttt tgtgcgcgat
     1621 ctgaataaga acaaagaggc aatccacgat ccgatttatt tcaaagggga atctgagggc
     1681 attaccgtag aatgtgcgtt ccagtatatc aacgaattcc aggaaaacgt gctcggcttc
     1741 tgtaataata tctacaacgc tgagggcggt acccatattt ccggtttcaa atcgaccttt
     1801 acgacgatta tgaacagcta tgccagagag atcggtgtct taaaagaaaa agacaataac
     1861 tttacgggat cggatatccg aaacggtatg accgctgtta tttcgatcaa gcacccggat
     1921 ccgcggttcg agggccagac aaaaacgaag ctggacaacc cggatgcggc aaaggccgcc
     1981 ggaaaagtaa cgggcgagga gattccgctg tatttcgacc gcaatctcga agtgctgaaa
     2041 aatgttcttg cctgcgctga gcgatctgcc aaaatccgca gaagcgaaga aaaagccaag
     2101 acgaatatgc tgaccaagca gaaatattcg tttgatacga acggcaaact ggcaaactgc
     2161 gagaaaaagg atccgtcgct gtgcgagatc tttatcgtcg agggagattc tgcgggcggt
     2221 tccgcaaaga cggcgcgtaa ccgtaatttc caggcgattc tgccgatccg cggtaagatt
     2281 ctgaacgtag agaaggcttc tattgataaa gttctggcaa atgccgagat caaatccatg
     2341 atcaatgcat tcggctgcgg cttttccgag ggttacggca acgacttcga tatcacaaag
     2401 ctccgttacg ataaaattat catcatggca gatgccgatg tcgacggtgc gcatatttca
     2461 acgctgctgc tgacattgtt ctaccgtttc atgccggagc tgatctacga gggacatgtc
     2521 tatatcgcga tgccgcctct ttacaaggca atgccgtccc gcggaaaaga agagtatctg
     2581 tacgacgaca aagcactgga aaaatacaga aagacacaca aagaaaaatt tacgctgcag
     2641 cgctacaaag gtcttggcga gatggacccg gagcagctct gggagacgac actgaatccg
     2701 gagacgagac ttttgaagcg tgtggagatc gaggatgccc gtatggcctc cgatgtgacg
     2761 gaagtactga tgggaaccga ggttccgccg cggaaagcat ttatttatga gcatgcaacc
     2821 gatgcagagc tggatattta acaggaagga caaaggaaga tgaaagaaaa atcagaagat
     2881 cagattatac ggaccgaata ttctgaggtc atgcagaaat cttatattga ttatgcgatg
     2941 agcgtcatca tttcccgagc gcttccggac gtgcgggacg gtttaaagcc ggtacagcgc
     3001 cgtactctgt atgacatgta tgagctcggc atccagtacg acaagccgtt ccgaaagtgc
     3061 gcgcgtatcg tcggcgatac gatgggtaaa taccatccgc acggcgacag ttccatttac
     3121 gatgctcttg tcgtaatggc acaggatttc aaaaaagggc agccgcttgt ggacggtcac
     3181 ggaaacttcg ggtcgattga gggtgacggt gccgctgcaa tgcgatacac cgaggcgaga
     3241 ctgcagaaaa ttacacagga ggtctattta aaggatctcg acaaagacgt tgtcgatttc
     3301 gtaccgaact tcgacgagac ggaaaaagag ccgtctgttc tgccggtgcg cgtgccgaac
     3361 cttctgttga atggcgcaga cggtatcgct gtcggtatgg caacaagcat tccgccgcac
     3421 aacctgtgtg agattgtgga tgcggtcaag gcctacatga aaaacaacga catcaccgtc
     3481 aaaggtctga tgaaatacat caaaggaccg gatttcccga ccggcggtat tgtcgtcaat
     3541 caggatgacc tgctgtcgat ctacgaaaaa ggtgtcggaa agattaaaat ccgcggaaag
     3601 gtggaagtgg agaaaggcag aaacggaaag cagtatcttg tgatcaccga aattccgtac
     3661 ccgatgatcg gtgcgaacat cggaaagttc ctgaacgatg tggcggggct tgtggagagc
     3721 aagaaagcgc cggaaatcgt cgacatttcc aaccagtcct cgaaagaagg aacacgcatc
     3781 gtgctcgaac tgaaaaaagg cgcggatgtg gagcggatca cgaatctgct gtacaagaaa
     3841 acacgtctgg aagatacgtt cggtgtcaac atgctcgccg tggcagatgg aagaccggag
     3901 acgttaagcc ttaaacaggt gatcgagcat cacgttgatt tccagtttga agtggcaacc
     3961 cgcaaatacc agaaccttct ccgcaaagag atggagcgcc gcgaggtgca ggaaggtctg
     4021 atcaaggcat gcgatgtgat cgatctgatc attgagatcc tgcgcggaag caagacacag
     4081 aagcaggtca aagagtgtct gattatgggt gtgaccgatg gtatccgctt caaatcaaag
     4141 tcaagtgagc gtatggcagc acagctctgc tttacggaac gccaggcaac ggcgattctg
     4201 gagatgcgtc tgcagaaact gatcggactg gaagtggaag cactcttaaa agatcatgag
     4261 gcgactgtgg cgaagattac gaagtacgag gatatcttaa atcattacga ctcgatgtcg
     4321 gaagttatta tggaagatct gacgcagatt aaaaaagagt acggccacaa gcggaaaacg
     4381 gcgattgaga atgctgaggc gattgttctt gaggagccga aggtcgagga gatggatgtt
     4441 gtcttcctga tggaccgctt cggttatgcg aagacgatcg atacgtctgc ttacgagcgg
     4501 aacaaagagg ctgtgcacaa cgagaacaaa tatgtgttcc agtgcaaaaa caccgataag
     4561 atctgcgtct ttacggatct tggcatgatg cacagcatca aagtaatgga catcccgtat
     4621 ggaagattcc gtgacaaggg aacgccgatc gacaaccttg gaaattacag cagccagaag
     4681 gagcagatga tttacgtcga tgcactggaa aatatcaaga acaataagct gctgttcgtc
     4741 agcgcggccg gtatggtaaa acttgtcgat ggatccgagt ttgatgtcgt aaaacgaacg
     4801 atcgcggcga cgaagctttc atccgaggag gaccgtctga ttcttgccga ggtctgccag
     4861 gagcaggagt atgtggttct gcagacgaaa aacggtgtct tcctgcgctt ccttacgagt
     4921 gaagtgccgg agaagaaaaa agcagccgtt ggtgtgcgtg gtatccgtat ggcagaggac
     4981 gacgcggtag agcatgcgta tctgctggca agccggatgg attacacgat cacgtatcac
     5041 gacaaagaga tttctctgac agcgaagatt aagctggcaa aacgagatac caagggaaca
     5101 aagatccggg tataacgcag agttttgttt ataaccatga gaagcacgct ccgcgggttt
     5161 ttcgagagat cacttggcaa taaaaggaca cctgcccgat accagaacgg tacgggcagg
     5221 tgtccttttt gcataatttt ttagttattt gaaaaactgt gggaagtctt tcttggggtt
     5281 cttctttcaa tggcaacgta cggacgatga tgaccgacat atttataagc cggacggatg
     5341 atctttcctt cgttgacaag ctcttcgatc cggtgtgcgg accagccgga gatacgggag
     5401 atagcaaaga tcggggtgta gagctctttt ggaaggccaa gcattgtgta gacgaaaccg
     5461 ctgtagaaat caacatttgc acagactggt ttgagcagac ggcgcttttt ggcgatacac
     5521 tcgccggcaa tgcgttctac agactcatag cgagcaaatt ctttctcgcg gtgcttctca
     5581 gcggaaagct tctttgcata ttctttcaga atcacacaac gtggatcgga cagggtataa
     5641 acagcatggc ccataccata gataagacca gttccatcga atgcttcacg atcaagtatt
     5701 ttggtcaggt aggtgcgcag ttcgttttcg tcttcccagt ttgtaacatt ctcttcaata
     5761 ttttcaaaca tctccataac cttcaagttg gcaccgccgt ggcgcggtcc ttttaaggaa
     5821 cttaaagaag ccgcaacagc agagtaggta tcggttccgg aggaggagac aacgtgtgtc
     5881 gtaaatgttg agttatttcc gccgccgtgg tctgcgtgaa gaattaacgc aacatcgaga
     5941 acctttgcct ccagagcagt gtaatctcca tccgggtgaa gcatctgcag aatgttttca
     6001 gcgatcgaca gctccggttt cggatttcgg atgatcaggt tctggttttc gtggtagtgg
     6061 cggtatgcat ggtagccgta aacggcgatc agcggcattt tggcgatcag actcaaagcc
     6121 tggcggagga cgttcggaat ggaaatgtca tccggctttt catcgtagga atacagggtc
     6181 agcacacagc gctgcagagt attcatcatg tttttgctgg ttgccttcat gacaacgtcg
     6241 cggacaaaag tgtccggcag ctcgcggtag atacttaaaa tttcgttgaa atcatttaac
     6301 tgcggttctg tcggaagaga accgaacaga agaagatagg taatctcttc aaaaccgaag
     6361 cgtcgtcctt ccataccttt gatcagatct gcgacgttat agccctgaaa atagagacgg
     6421 ccatcggtcg gaactttttc gccggtgcgc tcatcaatat cgaagccgat aacatcggaa
     6481 atttcggtca ggccggtgag aacaccttta ccagtggaat cacgcagtcc ccgttttaca
     6541 tcgtactcag tgtataaaga taaatcaatc tttccggaat ctttcgtata ctctacaagc
     6601 tgactgagct tgtcttccag attatcgtat tgcatggtca tgacaacagc ccctttcttc
     6661 ctttagttaa aaactatact tacttttttg gcggttgtca atgcttttct ttcaaaatac
     6721 attaattttt ataagggaaa gggatggttt tccttaattt ttgaaagtaa ggacaaagtt
     6781 tatgcataaa atttgcgcca gggaggtgaa aacaacaggg atggaaacgg cgggaaatca
     6841 gtggggcgga acaaatttta gaaaaagatt cataaatttc aaaaaaatgc ttgcaatttc
     6901 aaaaaaatac ggtataataa tgattgtgtc attcaggaaa tgaatttcac aggccgaaat
     6961 agctcagccg gtagagcact tcactcgtaa tgaaggggtc gtcagttcgn nnnnnnnnna
     7021 ttctttagaa acccgcagga cgtaaggttc tgcgggtttt ctggtatcat gagaaatatg
     7081 acagaaatac gaacgccctt tgcaggaaaa agatctgctg caaagggcgt ttgctgttac
     7141 aagtttcagg aaaatcagtt cgtacagcat atcctgacgg agcttttggc caggcacttc
     7201 aaagagcaca tcagctgttg ccattttcct gtgctgccac ccgtgcgtcg cgttcccggc
     7261 gggaaaagac gcagccgcag tagtcctggc ggtagaggcc gtattcatgg gaaagttcga
     7321 tggaacgctg gtagccgccc cgtttcttga aatctgaatt gagatatggg atcccgtatt
     7381 cggcggcaag ccgttctccg atttcattca gcttttccgc gtttttcagc gggctgatgg
     7441 agagggttgt tgtaaaatag tcgagtttga gctgctttgc catttcggcc gcttcgcgga
     7501 gccgcatttc atagcagaca aaacagcgct cgccgccctc tggcacattt tccatcccac
     7561 gtgtcagttt atagaaccgg tcagaatcgt acgggccggg gcagaaggaa atcggatgca
     7621 cggcgggaag agaatggatc agacgctgct gttcctccac gcggtgctcg tactccgtcg
     7681 gcggatagat gttgggatta tagtaaaaga gcgtaatgga aaaatactgc gacagatatt
     7741 ccagcacgta actgctgcac ggcgcacagc agctgtgcag cagcaaggtc ggaacttcac
     7801 cgtgaagtcc tgcaatcttc gcatccagct ccttctggta atttctttta ttggccataa
     7861 tgtatcttca aacctcctgg tatctttcaa tcctttctat tatagcctca gcgcggctgg
     7921 gatgcaagct gcatatgata tcttatagat agaggaggat gcaaaaaaat ggatgatcaa
     7981 atcctgtcat tagagcatat atcactgtcc tatcacacca tgaaaggaga aacaccggcg
     8041 ctgtgcgatc tgaccttttc ggtaaccccg ggggaatttg ttgctcttgt cggaccgagc
     8101 ggctgcggaa agagcacgat cctgaacttg atttccggtc ttttaaagcc ggaggcggga
     8161 actctgctga gccgcggacg gccgatcacg gaggcggatc tccatatcgg ctacatgctg
     8221 cagagggacc acctgtttga atggcggacc atctacagca acgttctgct gggccttgag
     8281 atttcgcgca cgctgacacc cgagcggaaa gaaaaagtcg gggagatgat ggagacatac
     8341 ggcctgaaat ccttttccaa tgcccgtccg tccgaacttt ccggcggtat gcggcagagg
     8401 gcggcactga tccgtacact ggcgatggag ccggatcttc tgctgcttga cgagccgttc
     8461 tccgctctcg actaccagac gaggctgaac gtctgtgatg atatcggaaa gatcatcaaa
     8521 aaagagggaa agacagcaat tctcgtgaca cacgatattt cggaggccat cagcatggcg
     8581 gaccgtgtga ttgttctggc ggcacggccg gcatatgtga aaaaaaagat tccaatccag
     8641 tttgcgatgg aggagcgcac gccgatgaaa gcgcgcagtg cgccggaatt taaaacgtat
     8701 ttcaatgcca tctggaagga gctgaatgaa aatgcgtgag caggcaaaaa cgccggagag
     8761 cagctttcag agggcgtatc tccggcagaa aaaaagagaa cgggagcagg tgcgggtgtt
     8821 tcgggctttg cttctcgtca gttttctggt attatgggag gtggcggcgg atctgagatg
     8881 gatcgatccg tttatctttt ccagccctgt gcgggtggga aaaacgttcg tgaccctgct
     8941 gacggagcag aaccttctgc gccatatcgg gatcacggtg tatgagacgg gaatcagctt
     9001 tctgctcgtc tcgacacttg gcatggcgtt cgctgtgctt ttgtggctct ttccgcgctt
     9061 ttctgcggtg atggagccgt acctggtagt cctgaacagc ctgccgaaat cggcgctcgc
     9121 cccgcttctc attgtgtgga tgggggcaaa cgtgaaaacg atcatcacgg ccggcatttc
     9181 cgttgcgata ttcggctcgg tgatcagcat ttacagcagc tttcagagtg tggagccgga
     9241 tatgctccgc ctgattgcaa cactcggcgg aacgaagaag gatatgctgt tccgcgtcgt
     9301 gctgccgtgg tcgtttccgg ggctgctcgc cgtgtggaag gtcaacatcg gcctgtgcct
     9361 cgtcggtgtt gtaatcggag agtttatcgg ggcaagagag gggcttggct atctgattat
     9421 ctacggcagt caggtgttca gatatgcaca cgcaagagaa gggaggatga cagatgattg
     9481 gaatcgtatg tctggaagaa cgaagaggaa tgcagtttgg cggacgacgc gtttcgcggg
     9541 accgcgtggt aacggaaaaa atctgcgaac tgtgtgaggg gaaaacgctc tggatgaatc
     9601 cggcttcgga aaagctgttc gcggagcaga aggagagtgg gaaggtaaca cttcaggcgg
     9661 cggcagattt tctggagcag gcaggcgaag gagaatactg ctttgtggag aatacgggac
     9721 tgcaggctgt ggaagcgaag atagagaaaa tcatcgtttt ctggtggaac cggcattatc
     9781 cgtctgaccg gaaattcgac ctggatcttt ccaaatggaa taaagtgtcc gaggaggagt
     9841 ttgcaggcta ttcccatgag aaaataacga aagaggtata tgagaaatga aaaaactaag
     9901 aaaacagctt ctgctgctgg cggcgcttct tgttctgaca ctgaccggct gtgaggcgca
     9961 ggatcttctc tcgacggaga gagcagattc cggttcggag gttacggccg cttatacgat
    10021 gacggaatcc cagattaccg atcaggcacc gacggccgtt tcagttcttg atgttccgga
    10081 gttttccggt gaaccgtatg tcgttttaaa cggaaatgag ccggatttta ccgatgagga
    10141 aaagacgacc gagtcgtacg agcattacag cgatctggac agcctgggac ggtgcggcgt
    10201 ggcagaggcg aatatcggcc aggatctgat gccgacggag aagcgcggcg cgatcggcca
    10261 ggtaaagccg acgggctggc atacggtaaa atacgatcag gtggaaggta aatatctgta
    10321 caaccgctgc catctgatcg gataccagct gaccggtgag aatgcaaacg agaagaatct
    10381 gatcactgga acacgttatc tgaatgtcga gggcatgctg ccgtttgaaa acatggttgc
    10441 cgactacgtc aaagagaccg gaaaccatgt gctgtaccgc gttacaccaa tttttaccgg
    10501 ggacgacctt gtagcggacg gtgtggagat ggaggcactt tccatggaag atgacggcga
    10561 gggcctttcc ttccatatct tcgcatacaa caatcagccg ggcatctcga ttaattatgc
    10621 aacgggagac agcacgttaa gcgaaagcag cggaacgatg acggatcagc aggaatatgt
    10681 catgaacaca tccagcatga aattccacct gccgtcatgc tccagcgtca gctcgattaa
    10741 ggatgaaaac aaggcgacat atcagggacc gcgcgaggac ctgatcgccg aaggctatga
    10801 gccgtgcgga cggtgcaatc cttaaaaaga aattacaaaa agagggtcgg aactttccgg
    10861 tccttttttc tatatagtaa agagaagcat ctggaaagtg agacaggaga tggaagagga
    10921 gaagctgcgt gcggtttatt atgcaaggtg cagcaccgag gaggaaaacc agaaggatgc
    10981 gctggtaagg caggcggcgg aggcggaaga ggcaatccgg gcaagaggat ggaggcttgt
    11041 ggatgcctac gtggagagcc gctccggtac gagccggaag ggcagacggg aatataaccg
    11101 cctgtacgag gatctcgagc agaaccggtt tgatgtcgtc gtaatcaagt cgcaggaccg
    11161 cctgatgcgg aacacgcggg actggtatct gttcgcagac cgcttaaact gcgccggaaa
    11221 gcggctgtat ttttatctgg agaacacatt ctattcgccg gaagacgcgc ttctgaccgg
    11281 cattaaggcg attctggcgg aggagtacag ccgggagctg agcaagaaga tgaatctggc
    11341 gcaccagaaa cggcagcaga ataatggaaa gccggtgctg acctcaaaaa gttatggtta
    11401 ccggcggacg gcagacggag cgattgagat catcccggag gaggccgcag tgaagcgccg
    11461 tatgtacgaa ctctgtgcag acggtctcgg cggacggaaa attgcggcag ttctggcagc
    11521 ggagggcgtc cgcagccgcg ccggaaaacc attctctgga acagacatta tccgcatggt
    11581 gcggaatccc atgaataaag gcaccgtagt catgaacaag aagcattacg attttaacac
    11641 cggaaaaacg ctccctacac cgccgggcgg gcagtacgtc tatgcgggga aaattcctgc
    11701 tatcgtctct gaagagctct ggatagcggc aaatcgggcg gctgacgagc gtctctgccg
    11761 caaaaaggtg ggcaaatctg ttcagacttg actgggtaat cctgtccatc ctgatcctct
    11821 gcctcgttgc ggcaggattg tacggcatct tgggctggct ggaaaaaaac tacacaaaac
    11881 gccgaaattc gggaaagtag gtgatttgac ttgcaaagga agaggggagt cttgtataat
    11941 aatacatgat aatttaaaaa acttacagga aaagaggaat gaaaatggca cagttatggg
    12001 gcggacggtt tacaaaagag accgacaagt tagtgtataa ttttaatgca tccatcagct
    12061 ttgatcagaa attctataaa caggacatca aaggcagcat ggcacatgta aagatgctgg
    12121 caaaacaggg catcctgaca gaagaggaga gagaccagat tcttgcagga ctggagggga
    12181 ttcttgccga tgtagagagc ggaaaactgg aaatcaccag cgaatatgag gatatccaca
    12241 gcttcgtgga ggcaaacctg atcgaccgta tcggagacgc cggaaagaag ctgcacaccg
    12301 gaagaagccg caatgaccag gtagctctcg atatgaaact ttatgtccgc gatgagatca
    12361 ccgagattga tgcactcgtc aaagaggtgc tggtaacact cgaaaaaatc atggaagaga
    12421 acattcacac ctatatgccg ggattcaccc atctgcagaa agcacagccg atcacactgg
    12481 cacatcacat gggcgcatat tttgaaatgt tccgccgcga cagaggccgc cttgcagaca
    12541 tctacaggag aatgaattac tgtccgctcg gttccggtgc gctggctgga acgacgtatc
    12601 cgctggaccg cgcttacacc gcagagctgc tcggctttga cggcccgacc ttaaacagca
    12661 tggattccgt atccgaccgt gactatgtca ttgagctcct cagtgctctt tctacgatta
    12721 tgatgcatct ttcccgcttc tccgaggaga tcatcatctg gaataccaat gagtaccagt
    12781 ttgtggacat cgacgacgca tacagcaccg gaagcagtat catgccgcag aaaaagaatc
    12841 cggatatcgc agagcttgtc cgaggaaaaa caggccgcgt ttacgcagct ctgatgtcaa
    12901 tgctcaccac catgaaggga atcccgctgg cgtacaacaa agatatgcag gaggataaag
    12961 agctgacctt cgatgccatc gacaccgtaa aaggatgcct ggctctgttc aatggcatgc
    13021 tttctaccat gcacttcaaa aaagaccgca tggagaacag tgcaaagaac ggtttcacga
    13081 atgcaacgga cgcagctgat taccttgttg gaaaaggcgt tccgttccgc gatgcacatg
    13141 gcattgtcgg ccgtctcgtt ctgtactgca ttgacaaagg aatctccctt gatgatatga
    13201 aactcgaaga gtacaaagcg atcagcccgg tcttcgagga tgatatttat gatgccatca
    13261 gcatgaagaa ctgcgtagaa cgacgcaaca cgatcggtgc gccgggagca gatgcgatgc
    13321 tggctgtcat cgagcagaat aaaaaatatt tacaggaaaa ttgaaaaaac agttgcaatt
    13381 ttgaaaaaat tggtatagta tgacatgaga cgcacaaatg tacgccacag aagaacaagg
    13441 agatgaaacc aatggagcag aaattgagag taggaatttt aggagcaacc ggtatggtag
    13501 gacagagatt tatttctctg ctggagaacc atccgtggtt tgaagttgtg accctggcag
    13561 caagtccgag aagtgccgga aaaacatacg aggaagcagt cggcggacgc tggaaaatgg
    13621 atactccaat gccggaagca gtgaaaaagc tcgttgtgat gaatgtaaat gatgtagagc
    13681 atgtggcatc taccgtagat tttgtatttt ccgcagttga tatgtcgaaa gacgagatca
    13741 aagcgattga agaagcatat gcaaagaccg agacaccggt tgtttccaac aacagcgcac
    13801 accgctggac accggatgtg ccgatggtag tgccggaaat caacccggaa catttcgatg
    13861 tcatcgagtt ccagaaaaaa cgtctgggca ccaagagagg atttattgcg gtaaaaccga
    13921 actgctccat ccagagctat gcgccggttt taactgcatg gaaagagttt gagccgtatg
    13981 aggttgttgc aaccacatat caggcaattt caggagcagg aaaaaccttc aaagac
//
LOCUS       DAOMUF010000036        13855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_973_length_13855_cov_3.026014, whole genome shotgun sequence.
ACCESSION   DAOMUF010000036 DAOMUF010000000
VERSION     DAOMUF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_13855_cov_3.026014"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /gene="rpsA"
                     /locus_tag="PLN77_03620"
     CDS             <1..1472
                     /gene="rpsA"
                     /locus_tag="PLN77_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRM43405.1"
                     /translation="TAEEAESFVPENGKPISIVAQTTFNYNKFKDLVEIFLKKSYDST
                     VLKTICNATEERQTEARAIARKVDAMFVVGGRHSSNTQKLYEICKEECKNTYFIETLV
                     DLESKPFQSFGRVGITAGASTPNKIIEEVQKMSEMSFEQMLDESFKTIRNGEVVEGTV
                     IDVKDDQIILNIGYKADGILTKNEYSNDSSIDLHTVAQPGDKMEVKVLKVNDGEGQVL
                     LTYKRLAAEKGNKRLEEAFENHEVLKAKVTQVLNGGLSVVVEETRVFIPASLVSDVYE
                     KDLSKYADQEIEFVITEFNPRRRRIIGDRKQLIVAEKKKLQEELFARIAVGDVVEGTV
                     KNVTDFGAFIDLGGADGLLHISEMSWGRVENPKKVFKVGETVKVLIKDINGDKIALSL
                     KFPEENPWLNAADKYAVGNVVEGCVARMTDFGAFVELEPGVDALLHVSQISREHVEKP
                     SDALKIGQEITAKVVDFNEEDKKISLSMKALENEEPVEE"
     gene            1620..2696
                     /locus_tag="PLN77_03625"
     CDS             1620..2696
                     /locus_tag="PLN77_03625"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43406.1"
                     /translation="MWEMKILGVDLYHVLAWFWLYSFCGWIWESSYVSIRQKKLVNRG
                     FVNGPLLTLYGTGAVMVYLILRPFEANVWALYFGGVIVATVLEYVTGVLMETIFHAHW
                     WDYSNQKFNFQGKICLSSSIAWGFFTLGLFYILQPIAVKVVNLVSDTTGEIIVSVVTV
                     CYLVDFGFSAAAAFQLGQKMRSLEKTWGEFLKYLQKSRFNQLAEEMNAKVQQYRAEYS
                     RDKMKEYLAEKRQTLSEAMEQMQKLEKERKEAEGRRNPLFESKNRLMEFGAEQKERLT
                     EFGAEQKERFAALGFDVRSDKMPDLKELLERFDQMTKPQLPTNWLMRLTTKRYLRAYP
                     HLQGTHHASLKKEEKKAEEKEEKK"
     gene            complement(2761..3849)
                     /locus_tag="PLN77_03630"
     CDS             complement(2761..3849)
                     /locus_tag="PLN77_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRM43407.1"
                     /translation="MQIIDSTPAALSADLQMYLRNGDVFFDIETTGLSWRTSHLYLIG
                     ALFFDPAADTFTLRQWFLDRPTEEKEMLVSFFTFLSDVKRLVHFNGTTFDLPYLTHKA
                     LFYQMEDPLSSIASLDLYQALRPFQSILGLSSMKQKNVEQYLSFPRKDQLNGKQLILV
                     YHDYLQTLDEKKLELLFLHNYEDVLGMGSVLELLALPALFHGDFSVQSCRFTGQTLEV
                     SLQPEREVPVFLSRVCADGSLTAFGSRVSLSLQTHTAELKYFFPDYKNYYYLPLEDQA
                     VHKSVGAFVDPEHRQKAKAANCYQRRTGTFLPQQKEFFTPAFREDFKSRPYYFEWSDA
                     FLSQEDLLKEYLCSELQLFFKDDSKTRK"
     gene            4023..4637
                     /gene="ruvA"
                     /locus_tag="PLN77_03635"
     CDS             4023..4637
                     /gene="ruvA"
                     /locus_tag="PLN77_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRM43408.1"
                     /translation="MIAFIKGTVDSLSEGKMILESHGIGYELNIPASMFDAGVREGEE
                     LKIYTHMSVREDGISLFGFLSREDLEIYRMLIGVSGIGPKAALAVLSTLTADNLRFAV
                     LSEDVQAIAKTPGIGKKTAQKLILELKDKFDLQEAFEQKLAGNQAAAAVRQAGEPDAF
                     QDAVQALTALGYSGTEALQAVKKVEITEGMDSEAVLKAALKHMF"
     gene            4678..5688
                     /gene="ruvB"
                     /locus_tag="PLN77_03640"
     CDS             4678..5688
                     /gene="ruvB"
                     /locus_tag="PLN77_03640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRM43409.1"
                     /translation="MERRIITTDVLEEDVKIEGSLRPQHLDDYIGQEKTKKNLKVYIE
                     AAKQRSDVLDHVLFYGPPGLGKTTLAGIIANEMGTHMKVTSGPAIEKPGEMAAILNNL
                     QEGDVLFVDEIHRLNRQVEEVLYPAMEDFAIDIMIGKGSSARSIRLDLPKFTLVGATT
                     RAGLLTAPLRDRFGVIHHLEFYTEEELANIILHSSKVLNVEVDPQGALEMARRSRGTP
                     RLANRILKRVRDFAQVKYDGRITKEVASFALDLLEVDRYGLDQTDRLLLTTIMEKFQG
                     GPVGLDTLAASIGEDAGTIEDVYEPFLIKSGFINRTPRGRVATEFAWHHLGLEAPRKI
                     EE"
     gene            5819..6250
                     /gene="zapA"
                     /locus_tag="PLN77_03645"
     CDS             5819..6250
                     /gene="zapA"
                     /locus_tag="PLN77_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRM43410.1"
                     /translation="MAVKNTTQVLIDGKIITLGGYESPEYLQKVAAYLNQKISELSQS
                     AGYNRLSVDTKHTLLALNIADDYFKAKSQVDTLEEDMEAKDRETYDMKHDLIAAEIQA
                     GDLKKELEEKRIELEHVRGEREELQRQLDKANKDLEDLLKA"
     gene            6308..8521
                     /locus_tag="PLN77_03650"
     CDS             6308..8521
                     /locus_tag="PLN77_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HRM43411.1"
                     /translation="MKTELLAPAGSMEALKAAVSAGADAVYLGGAAFGARAYAKNLDE
                     QEILSAIDYVHLRNRKLYLTVNTLLKEEELEEKLYPYLRPYYEQGLDAVIVQDMGVLK
                     AVRSWFPDLDIHASTQMTVTGSAGARFLESLGATRVVPARELSFAEIQKIHRTTNLEI
                     ECFVHGALCYCYSGQCLFSSLIGGRSGNRGRCAQPCRLPYEAYDKDNHRMGEPGDRYP
                     LSPKDMCTVELLPEIVKSGIMSLKIEGRMKKPEYTAGVVSIYRKYLDLYEKKPSRFHV
                     LPEDMKKLYELYNRDGFNKSYYTVRNGRDMMALKNEKEQENKKKQRRNEQLFYEIQRD
                     YIETEAKEPISGFLTLYPGQPAFLSAESGKYSVTAEAGMVEPAKKQPLTEERAKTQLE
                     KTGETPFYFKELDVCMDDNCFVPMQTLNELRRGVSDQLVKEMTEPYRRKAAEKPEQEV
                     KASGKPDQESRAEKKMELTASAETRAQWNALLEIPEITTIYAGMGCFKREIFEEQAEK
                     GILQAKELGKQVYLMLPHVVREGDLKEYRDTFRCLKEIGLGGFLIRNLESFSFLKEMG
                     MEKDIRLDYSVYTYNSRAQAFWQEQGVQRDTVPYELNEREIGKRDNTNSEMVVYGYLP
                     MMVSAQCVQKNLNGCNHSYSLVRLKDRMGKYFPVKSYCTSCYSVIYNSLPLGLVKEAD
                     EIRSMHPAAVRLNFTIETLEETKEIAAAFAGTYCKGIAVPAEQEYTKGHFRRKVE"
     gene            8548..10062
                     /locus_tag="PLN77_03655"
     CDS             8548..10062
                     /locus_tag="PLN77_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM43412.1"
                     /translation="MENTIVQLSKYVITFLMICFTVQSFAALKERDEDERHYILMRQI
                     IMIILMNFICFFVMFLQEGEVSTIQMFLLTMAYILGVQILYRLIYRKASMILVNNMCM
                     LLSISMIILTRLSVSKAMSQYKILAASTLLCFIIPVIVRKGKFLKNLTWIYAVLGIAM
                     LSVVMVLGFTSGGAKLSIEIHGITFQLSEIAKITLVFFMAGMLREDNSFGNVVKATVI
                     AAVHVLILVASTDLGTAFVFFMAYVVVIYVATRKARYPLLGLAGMSAACVVAYFLFSH
                     VRNRVALWQDPIGNWDISSQLAQGLFGMCSGGWFGTGLFEGRPDIIPVATKDFIFCAI
                     CEEMGIIFGICLILLCMSTFLLIINISMKMSKRFYKLIAMGLGTEYATQVFLTIGGTI
                     KFIPMTGITLPLVSYGGSSMFSTVLMLSIIQGLYILREDEGEELEKRQNKKKRNQQKN
                     DPGAKKKRRPDEETERRKKSGSRNDGQGTRQKYREQPDEFERRIEEQTENSLHW"
     gene            10010..11476
                     /locus_tag="PLN77_03660"
     CDS             10010..11476
                     /locus_tag="PLN77_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM43413.1"
                     /translation="MNSNGGSKSRPRTASTGKAANNGAGPYLIVSFLFVAMFLGLIAY
                     LVYFNVVRKEEFLNSSYNTRQNNYAERVIRGTIYSADGQELAKTTTDENGDEVRTYPF
                     GSLFAQVVGYTGKGNSGLESSYNYMLMESHTSKLKQVKNEFSDAKNPGDSLYTTLNTT
                     LQQAAADALDGYRGAVVVLEAKTGRVLANVSNPVFNPNTIDEDWEALNADDESGVFLN
                     RGLQGRYPPGSTFKIVTSLAYLRQNGTLDGFSFDCDGELTAGNYTIHCSGGEVHGPED
                     FAGAFAHSCNSAFAQIGLGLDKDAFRSLADSLYLNSRLDLELPTSKSSFDLDSTTADA
                     LVMQTSIGQGDTLVTPMEMALIASAVANDGEMIKPRYVDNIVSADGQAVKTFYKESLG
                     TVMSESEANTLTELMKGVVQSGTAVSLSDLPYNIAGKTGTAEHGSDGETPHSWFVGFS
                     NADDPDIVIAVVAENGGYSSEVAVPIARQVIEAYYAQQ"
     gene            11680..12156
                     /locus_tag="PLN77_03665"
     CDS             11680..12156
                     /locus_tag="PLN77_03665"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM43414.1"
                     /translation="MIEAVSCGGIVIYRGKILTLYKSYRNKYEGWVLPKGTVEPGETH
                     EQTAVREVYEESGVHGTIIKYVGKSQYSFSIPEDTVEKEVHWYLMVSSGYYSRPQREE
                     YFVDSGYYKYHEAYHLLKFSNERQILEKAYQEYLELKRANLWKVQPQAQGKYGKLV"
     gene            12140..12469
                     /locus_tag="PLN77_03670"
     CDS             12140..12469
                     /locus_tag="PLN77_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43415.1"
                     /translation="MVNWYKNLYVGDTAKKKQKKWMRRIENKKAVPGVWLLTLPSNQQ
                     NNLDVIPADLLLQPAVRRNCPMIVGLAVSREEAFELVEAIALETYRETGEVKIREWLL
                     NRIEGCE"
     gene            12466..13554
                     /locus_tag="PLN77_03675"
     CDS             12466..13554
                     /locus_tag="PLN77_03675"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HRM43416.1"
                     /translation="MIHILFMILKALGILLLVLLFLVLLIVCTVLFLPFCYRAQVLKE
                     EEGFACVKASGRVSWLFGAVALTASYEEQKPEAQILLFGASLETWKRRLKKIRRGEAS
                     VSRTEENETENAPEAEKTAEQKAEAKKEQTSAEQPAEQKTDTVKKQMPQRDAEKTQKA
                     PDQTEKQQKVTAQKEQPEQEQEPDAPKKSILERFFGRIEYLPEKLLNLVSRLLQTAFR
                     LLELPFRLLEKLEQKIQAGRRLKRKWESVKKFFRSKMFREALLHAKKEVLYFLKKAAP
                     KKVTGTVRFGFDDPALTGETLGILGMIYGKLPKDLSIQPDFEQEILRGDVRMKGSFQA
                     VTAAGIALRLFRDQNLRKTIRHFKHKEA"
     gene            13558..>13855
                     /locus_tag="PLN77_03680"
     CDS             13558..>13855
                     /locus_tag="PLN77_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HRM43417.1"
                     /translation="MAEKDNTFQNTVEALFRGMDSFITTKTVVGEAIHIDDTILLPLV
                     DVSFGVGAGAFAENAKNNAGGGMGGKISPSAVLVISKNGTRLVNIKNQDAVTKII"
BASE COUNT         3995 a         2999 c         3804 g         3057 t
ORIGIN      
        1 ataccgccga agaagcggaa agttttgtgc ccgaaaatgg aaaaccaata tctattgtgg
       61 cacagacgac atttaattac aacaaattta aagatttagt tgaaattttt ttgaaaaaga
      121 gttatgatag tactgtttta aagactatct gcaacgctac cgaggaaaga cagacggaag
      181 cgagagcaat tgccaggaag gttgatgcca tgtttgtcgt gggaggcaga catagttcaa
      241 acacgcaaaa gctgtatgag atatgtaaag aagaatgtaa aaatacttac tttatagaga
      301 cactcgttga tttggaatct aagccttttc aatcatttgg tcgcgtaggt attacagctg
      361 gggcatccac cccgaataaa ataattgagg aggttcaaaa aatgtcagaa atgagttttg
      421 aacaaatgct ggatgaatca tttaaaacaa tcagaaacgg agaagtcgtt gagggtactg
      481 taatcgatgt caaagacgat cagattatcc tgaatatcgg atacaaagcc gatggtattc
      541 ttaccaaaaa cgaatattcc aacgattctt ccatcgatct tcacaccgtt gcacagccag
      601 gtgacaaaat ggaagtcaaa gttctgaaag taaatgatgg agagggacag gtccttctta
      661 cttacaagag acttgctgct gaaaaaggca acaagagact cgaggaagca ttcgagaatc
      721 atgaagttct gaaagcaaaa gtaacacagg tactgaacgg aggattatcc gtagttgttg
      781 aggagaccag agtattcatt ccggccagcc ttgtatctga tgtatatgaa aaagatctgt
      841 ctaaatatgc agatcaggaa attgaattcg ttattaccga gttcaacccg agaagaagac
      901 gcatcatcgg tgaccgcaaa cagctcatcg ttgctgagaa gaagaaactg caggaagaac
      961 tgttcgcacg catcgcagtt ggcgatgttg tagagggaac tgttaagaat gttaccgatt
     1021 tcggcgcatt catcgatctg ggcggagctg acggactgct tcacatctct gagatgtcct
     1081 ggggccgcgt agagaacccg aaaaaagtat tcaaagttgg tgagactgta aaagttctga
     1141 tcaaagacat caacggcgac aagatcgctc tttccttaaa attcccggaa gagaatccgt
     1201 ggctgaacgc tgctgacaaa tacgctgtag gcaacgtagt agaaggctgt gttgcccgta
     1261 tgactgactt tggtgcattc gttgagcttg agccgggcgt agatgctctg cttcatgttt
     1321 cccagatttc cagagagcat gttgagaaac cgtctgatgc actgaaaatc ggacaggaaa
     1381 tcactgcaaa agtagttgat ttcaacgagg aagacaaaaa aatctctctg agcatgaaag
     1441 ctctggaaaa cgaagagcca gtagaagagt aagaataatt taacttgcag gtgctgcagt
     1501 tggatgatta gaatgccgta gacaaccggg tttcctgaac agacaggaaa cctgttgcct
     1561 gcggtatttt cttatcatcc tgtttttggc accagaaaag aaaaagggga tgaaagttta
     1621 tgtgggagat gaagattctg ggtgtggatc tgtaccacgt tctagcatgg ttctggctgt
     1681 acagcttctg cggatggatc tgggagagtt cctatgtatc gatccggcag aaaaaactgg
     1741 tcaaccgcgg ttttgtcaat ggtccgctgc tgacactgta cggcaccggc gctgttatgg
     1801 tatacttgat cctgcgcccg tttgaagcga acgtctgggc actgtatttc ggaggtgtca
     1861 ttgtagcgac ggttctggaa tatgtaaccg gcgtgctgat ggagaccatt tttcatgcgc
     1921 actggtggga ctacagcaat cagaagttta attttcaggg aaagatctgc ctcagcagtt
     1981 cgattgcctg gggattcttt acactggggc tgttctatat tctgcagccg attgccgtga
     2041 aagtagtgaa tcttgtttcg gatactacag gagagatcat tgtttccgtt gttaccgtct
     2101 gctatctggt agattttgga ttttccgcag ccgcagcatt ccagctggga cagaagatgc
     2161 gcagtctgga gaaaacgtgg ggagaatttc tgaaatatct gcagaaatcc cgctttaacc
     2221 agcttgccga ggagatgaat gcgaaagtac agcagtatcg tgcagagtat tccagagata
     2281 agatgaaaga atatctggcc gagaagcgcc agacactgtc tgaggcaatg gagcagatgc
     2341 agaagctgga gaaggagcgg aaagaagccg agggaagaag aaatccgctt ttcgagtcga
     2401 agaaccgcct gatggagttc ggagcagagc agaaggaacg cctgaccgag ttcggagcag
     2461 agcagaaaga acggtttgcg gcacttggat ttgatgtgcg cagcgataag atgccggatc
     2521 ttaaagagct gctggagcgc tttgaccaga tgacaaaacc gcagcttccg accaactggc
     2581 tgatgcgcct gacaacgaaa cgttatctgc gcgcctatcc gcatctgcag ggaacgcatc
     2641 atgcttctct gaaaaaggaa gaaaaaaagg cagaagagaa agaagagaaa aaataaagga
     2701 ctgcttggaa aggacagtcc cggaaaaacg gccgtgcatg caaaaacgtg tacggctgtt
     2761 ttattttctg gttttgctat cgtctttgaa aaagagctga agctcggagc agagatactc
     2821 tttcaggaga tcttcctgcg aaagaaaggc gtcggaccac tcaaaatagt aagggcggct
     2881 tttgaaatcc tcgcggaagg ccggggtgaa aaactctttc tgctgcggaa gaaatgtacc
     2941 ggtccgccgc tgatagcagt tggcagcctt tgctttctga cggtgttccg gatcgacaaa
     3001 cgcgccaacg ctcttgtgaa cagcctgatc ctccagcgga agataatagt aattcttata
     3061 gtccgggaag aaatatttca gctccgccgt atgcgtctgc agggagagag aaacccgtga
     3121 accaaaggct gtaagagaac catccgcgca gacacgggag aggaaaaccg gaacttcccg
     3181 ctcgggctgc agtgaaactt ccagtgtctg tccggtaaaa cgacacgact ggacagaaaa
     3241 atcgccgtga aacagggcag gaagcgcgag cagttccaga acagaaccca tgccgaggac
     3301 atcctcataa ttgtgaagga aaagcagttc cagtttcttt tcatccagtg tctgaagata
     3361 gtcgtggtaa accaaaatca actgttttcc gttaagctga tccttccgtg ggaatgagag
     3421 atactgttcc acatttttct gtttcattga agaaagtccc aggattgact gaaacgggcg
     3481 gagtgcctga taaagatcca gggatgcgat tgacgaaagc ggatcttcca tctgataaaa
     3541 cagtgctttg tgtgtgaggt aaggaagatc aaatgtcgtt ccattaaaat gcaccagccg
     3601 cttcacgtcg gaaagaaacg tgaaaaaaga gacaagcatc tctttttctt cggtcggtcg
     3661 atcaagaaac cactggcgca gtgtgaaagt atccgcggcc gggtcaaaga acagcgcacc
     3721 aatcagatag agatgcgagg tacgccagga aaggccggtc gtctcaatat cgaaaaagac
     3781 atcgccgttt ctcagataca tctgaagatc ggcagagaga gcagcggggg tagaatctat
     3841 gatctgcata aggttttttg tctcctttga aaaacttttt tgaatctaaa gatattataa
     3901 gacgaaaaaa tctgacatgc aagaaagaca gcgactaatc gttgcgtgat gagaaaagtt
     3961 ctgctataat agaatatgcg ttcgctttca aaaaaaagaa aacggcgctt tggaggaaaa
     4021 acatgattgc atttatcaaa ggaactgtag attctctttc tgaaggaaaa atgattctcg
     4081 aaagccatgg aatcggctat gagctgaata ttccagcgag tatgttcgac gcaggcgtcc
     4141 gggagggaga agagttaaag atatataccc atatgagtgt gcgggaagac ggaatttccc
     4201 tattcggctt tctgtcccgt gaagatcttg aaatataccg tatgctgatc ggcgtaagcg
     4261 gcatcggacc aaaagcggcg cttgcggtgc tgtccacgct tactgcagac aatctgcgct
     4321 ttgccgtcct ctcggaagat gtccaggcga ttgccaaaac accgggcatc ggaaagaaga
     4381 ccgcgcagaa gctgattctg gaattaaaag acaaattcga cctgcaggaa gcgttcgagc
     4441 agaagctggc aggaaatcag gcggctgcgg cggtacggca ggcaggcgaa ccggatgcct
     4501 tccaggatgc ggtacaggcg ctgactgcgc ttggctactc cggaacggaa gcactgcagg
     4561 cagtaaagaa agtcgaaatc accgaaggca tggacagcga ggctgtgctg aaagcggcat
     4621 tgaagcatat gttttagcag atggtcctaa attgtggaag aaggaaacct tataacgatg
     4681 gaaagaagaa ttatcacaac tgacgttctc gaggaggacg tgaaaatcga aggttcgctc
     4741 cgtccccagc acctggacga ctacatcgga caggaaaaga cgaaaaagaa cctgaaagta
     4801 tatattgaag cagcaaaaca gcgaagtgat gttctcgatc atgtgctgtt ttacggccct
     4861 ccgggacttg gaaaaacaac gctggcgggc atcattgcaa atgagatggg aacccatatg
     4921 aaggtaacga gcggcccggc gatcgaaaaa ccgggtgaga tggcagcgat cctcaacaat
     4981 ctgcaggagg gcgacgtcct gtttgttgat gagatccacc gcctgaaccg ccaggtcgag
     5041 gaggttcttt acccagcgat ggaggatttt gcgattgata tcatgatcgg aaaaggctct
     5101 tcggcgcgct cgatccgcct ggatctgccg aagtttacgc tggtcggcgc aacaacgcgt
     5161 gcaggtcttt tgactgcccc gctccgcgac cggttcggtg tcatccacca tctggaattt
     5221 tacacggagg aagaattggc caacattatt ctgcactcct caaaagtact gaatgtggag
     5281 gtggacccgc agggagcgct tgagatggcg cgccgttcca ggggaacacc gcgtctggca
     5341 aaccggattt taaagcgtgt ccgcgacttt gcccaggtga aatacgatgg aagaatcaca
     5401 aaagaggtcg catcatttgc tctcgatctg ctggaagtag accgttacgg actggatcag
     5461 actgaccggc tgcttcttac caccattatg gaaaagttcc agggcggtcc ggtgggactc
     5521 gataccctgg cagcatcgat tggagaagat gccggcacca tcgaggatgt ctacgagccg
     5581 ttcctgatca aatccggatt tatcaaccgc actccgcgtg gaagagtggc aacggaattt
     5641 gcctggcacc acctggggct ggaggcaccg cgtaagatcg aagagtaaaa gccctgaatc
     5701 tttcattttc tgtcgctttt tgcagaaaaa ggttgttttt cttccatttt cggtatatga
     5761 taggttatat tagccgggag tttggtccgg ttaaattcag tggaggaaaa gcaaaagaat
     5821 ggctgtaaaa aatacaacac aggttttaat agatggaaaa attatcacac tgggaggcta
     5881 tgagagcccg gaatatctgc agaaagtagc tgcttatctg aatcagaaga tcagcgaact
     5941 ttcccagtca gccggataca accgcctgtc ggtggatacg aagcacacac tgcttgcact
     6001 gaacattgca gacgactatt tcaaagcaaa aagccaggta gataccctcg aagaggatat
     6061 ggaagccaaa gaccgggaaa cctacgatat gaagcacgat ctgatcgcag cggaaatcca
     6121 ggccggcgat ctgaaaaaag aactggaaga gaaacgaatc gagctggaac atgtgcgcgg
     6181 cgagagggaa gaactgcagc ggcagcttga taaggcaaat aaggatctgg aagatttatt
     6241 aaaagcgtaa aagaaggaaa aacgggggat tgcggaacag gtaatcgccc gtttttagga
     6301 gataaagatg aagacagaat tattagcacc agccggttcg atggaagcgt taaaagcagc
     6361 agtttccgcc ggtgcggatg ccgtctatct cggcggagcc gcattcggcg caagagcgta
     6421 tgcaaaaaat ctggatgagc aggagattct ctcagccatc gattacgtcc atctgcggaa
     6481 ccggaaactg tatctgacgg tcaacaccct cttaaaagag gaggaactgg aagagaaact
     6541 gtatccgtat ctgcgtccgt actatgagca gggactggat gccgtgattg tccaggatat
     6601 gggcgtttta aaggcggtcc gctcctggtt cccggatctc gacattcatg cgagcacaca
     6661 gatgacagtg acaggatctg cgggagcgcg tttcctggag agccttggcg cgacgcgtgt
     6721 ggtaccggcc cgtgagcttt cgtttgcgga gatccagaaa atccaccgga cgaccaacct
     6781 tgaaattgaa tgcttcgtcc acggggcgct ctgctactgc tattcgggac agtgcctttt
     6841 ttccagcctg atcggcggaa gaagcggaaa ccgcggaaga tgcgcgcagc cgtgccgtct
     6901 gccgtacgaa gcgtacgata aagacaacca ccgcatgggg gagccgggcg accgctatcc
     6961 gttaagcccg aaggatatgt gtacggtaga actgctgccg gagattgtaa aatccgggat
     7021 tatgtccctc aaaatcgaag gcagaatgaa aaagccggag tatacagcgg gcgttgtcag
     7081 catttaccgc aaatacctcg atctgtacga aaaaaagccg tcccgtttcc acgttctgcc
     7141 ggaggacatg aaaaagctgt atgagctgta taaccgtgac ggtttcaaca aaagctatta
     7201 taccgtaaga aacggacgcg atatgatggc gctgaagaac gaaaaagagc aggaaaataa
     7261 aaagaaacag cgcagaaatg agcagctctt ttatgagatc cagagagatt atatcgagac
     7321 tgaggcaaaa gagccgattt ccggctttct gaccctgtat ccgggccagc cagcgttctt
     7381 aagtgcagaa agcggaaaat acagcgtaac agcagaggcg ggcatggtag aaccggcaaa
     7441 gaagcagccg ctgaccgagg aacgcgcaaa aacacagctg gaaaagacag gcgagacacc
     7501 attctatttc aaagaactcg atgtctgtat ggatgacaac tgtttcgtgc cgatgcagac
     7561 gttaaacgag ctgcggagag gcgtttcgga ccagctggtg aaagagatga cagagccata
     7621 ccgcagaaaa gcagcggaaa agccggaaca ggaagtaaaa gcgtctggaa aaccggacca
     7681 ggagagcagg gcagaaaaga aaatggagct gaccgcatct gccgagacga gagcccagtg
     7741 gaatgcgctg ctcgagattc cggagatcac gacgatctac gccggcatgg gatgcttcaa
     7801 acgggaaatc ttcgaagagc aggcggaaaa aggcatcctg caggcgaaag aactgggaaa
     7861 acaggtttat ctgatgctgc cgcacgttgt gcgtgaaggc gatctgaagg aataccgcga
     7921 cacgttccgc tgtctgaagg agatcggtct tggcggtttc ctgatccgca atctcgaaag
     7981 cttttcgttt ttgaaagaga tgggaatgga aaaggatatc cggctcgatt acagcgttta
     8041 cacgtacaac agccgtgcgc aggcattctg gcaggagcag ggcgtgcagc gtgataccgt
     8101 tccgtatgag ctgaacgagc gggaaatcgg aaaacgtgac aatacgaaca gcgaaatggt
     8161 ggtctacggc taccttccga tgatggtttc cgcccagtgt gtccagaaga acctgaacgg
     8221 ctgcaatcac agttattctc tggtacgctt aaaagaccgg atgggaaaat acttcccagt
     8281 gaagagttac tgcacctcgt gctacagtgt gatctacaac agccttccgc tcggcctggt
     8341 aaaagaagcc gatgagatcc ggtccatgca tccggctgcg gtgcggctga attttaccat
     8401 cgagacactg gaggagacaa aagaaatcgc cgctgcgttt gctggaacgt actgtaaagg
     8461 aatcgcagtt ccggccgaac aggaatatac caaaggccat ttccgccgga aagtagagta
     8521 aacggtgaga aaaaataagg ggaatttatg gaaaatacca ttgttcagtt atcaaaatat
     8581 gtgattacct tcctcatgat ctgttttacc gtgcagtcgt ttgctgcact caaagagcgg
     8641 gatgaggatg agcgccatta cattctgatg cggcagatca tcatgatcat tctgatgaat
     8701 ttcatctgtt tcttcgtgat gttcctgcag gagggcgaag tatcaacgat tcagatgttt
     8761 ctgctcacga tggcgtatat cctgggcgta cagatcctgt accgcctgat ttaccggaag
     8821 gcatcgatga tcctggtaaa caacatgtgt atgctgctct cgatcagcat gattattctg
     8881 acgagactga gcgtgtcaaa ggcgatgtcc cagtacaaaa ttctggcggc atccacactg
     8941 ctctgcttca tcattccggt cattgtccga aagggcaagt tcctgaaaaa tctgacctgg
     9001 atttacgctg tgctcggaat tgccatgctt tccgtcgtta tggtgcttgg ctttacttcc
     9061 ggcggtgcga agctgtcgat cgaaatccac ggcattacgt tccagctctc cgagattgcc
     9121 aagatcacac tcgtgttctt tatggcaggc atgctgcgcg aggataattc ctttggaaac
     9181 gttgtgaaag caacggtcat tgcggcagtt catgtgctga tccttgttgc atccaccgac
     9241 cttggaactg cgttcgtctt ctttatggcg tacgtggttg taatctatgt ggcaacgaga
     9301 aaagccagat atccgctttt gggacttgcg ggaatgagtg ccgcctgcgt tgtggcgtat
     9361 ttcctgtttt cccatgtacg gaaccgtgtg gcactctggc aggatccgat cggaaactgg
     9421 gatatcagca gccagttagc acagggcctc ttcggcatgt gctccggcgg atggttcgga
     9481 accggccttt tcgagggacg gcctgacatt atcccggttg caacgaaaga ctttattttc
     9541 tgtgcgatct gtgaggagat gggcattatc ttcggaatct gcctgattct gctgtgcatg
     9601 tccacattcc ttctgattat caatatttcc atgaagatga gcaaacggtt ctacaaactg
     9661 attgccatgg gtcttggaac ggaatatgcg acacaggtgt tcctcaccat cggaggaacc
     9721 attaaattta ttcctatgac aggtatcacg ctgccgctgg tcagctatgg aggaagctct
     9781 atgttctcca cggtgctgat gctgtcgatc attcagggat tgtacatctt aagggaagac
     9841 gaaggtgagg agcttgagaa aagacaaaac aagaagaaac gaaaccagca gaaaaacgac
     9901 ccaggcgcca agaaaaaacg acgcccggac gaagagaccg aaagacgaaa gaagagcggc
     9961 agccggaacg acggacaggg aacccgccag aagtaccgcg agcagccgga tgaattcgaa
    10021 cggcggatcg aagagcagac cgagaacagc ctccactggt aaggcggcaa acaacggcgc
    10081 agggccgtat ctgatcgttt cgttcctgtt cgtggccatg tttcttgggc tgatcgcgta
    10141 tctcgtttac tttaatgtcg tgcggaaaga agaatttctg aatagttctt acaatacgag
    10201 acagaataac tatgcagagc gcgtgatccg cggaacaatc tattctgcgg acggccagga
    10261 actggcaaag acgacgacag atgaaaacgg ggatgaggta agaacgtatc cgttcggcag
    10321 cctgtttgcg caggtggttg gctacacggg aaaaggaaac agcggcctcg aatcttccta
    10381 caactatatg ctgatggagt cccatacaag taagttaaag caggtaaaga atgaattcag
    10441 cgatgcgaag aacccgggag acagtctcta tacaacgctg aatacaacac tccagcaggc
    10501 cgcggccgat gcactggacg gataccgcgg tgcagttgtc gtgctggagg caaaaacggg
    10561 ccgtgtactt gccaatgtga gcaatccggt ttttaatccc aatacgattg atgaggactg
    10621 ggaggcactg aacgcagacg atgagagcgg cgtgtttctg aaccgcggtc tgcagggaag
    10681 atatccgccg ggatctacgt tcaagattgt tacttcgctt gcgtatctcc gtcagaacgg
    10741 cacactggac ggtttttcgt tcgactgtga cggtgagctg acagcaggaa actatacgat
    10801 tcactgctcc ggcggtgaag tgcatggacc ggaagatttt gcgggtgcgt ttgcacattc
    10861 gtgcaactct gcctttgcac agattggcct tgggctggat aaagatgcct tccgctcgct
    10921 ggcggattcc ctgtatctga acagccgtct ggatctggag cttccgacca gcaaaagttc
    10981 tttcgacctt gattcgacaa cggcggatgc tcttgtgatg cagacctcca ttggccaggg
    11041 cgatacgctg gtaacaccga tggagatggc actgatcgcc tccgcggttg caaatgacgg
    11101 agaaatgatt aaaccgcgtt atgtggacaa cattgtgtct gcggacggac aggcagtcaa
    11161 aaccttttac aaagagtctc ttggcacggt aatgtccgag agcgaagcca atacgctgac
    11221 agagctgatg aaaggcgttg tccagagcgg aactgcagtt tcactttcgg atctgccgta
    11281 caatatcgcc ggtaaaacag gaaccgcaga acacggaagc gacggagaga caccgcattc
    11341 ctggtttgtc ggattctcca atgcggatga tcctgatatt gtcattgctg ttgtggcaga
    11401 aaacggcgga tattcgagtg aagtcgctgt accgattgca agacaggtaa ttgaagcata
    11461 ttatgcacag cagtaaacga tgaaaaaacg cgttcgaacc ggaaaggatg ggttcgggcg
    11521 cgttttctga atttttagaa aaatgttaga aaaaggatgc ggttttaagg ttgcgcgggg
    11581 gcgggaaaag aggtatactt tttaacagaa gatctggcgg caagcgggaa gaaaagagct
    11641 gtcagagaat ggaaaaaagc cagaaggagc ttaaaacgta tgatcgaagc agtaagctgc
    11701 ggaggtatcg taatctaccg tggaaaaata ttgacgcttt acaaaagcta ccgcaacaaa
    11761 tatgaaggct gggttctgcc gaaaggaacc gttgagccgg gagaaaccca tgaacagact
    11821 gctgtccgcg aggtgtatga ggaatccggc gtgcacggaa ccatcatcaa gtacgtggga
    11881 aaaagccagt acagcttcag cattccggag gacaccgtgg aaaaagaggt gcactggtat
    11941 ctgatggtct catccggcta ctacagccgt ccacagagag aagaatactt tgtggattcg
    12001 ggctattaca agtatcacga agcatatcat ctgctgaagt tttccaatga acggcagatt
    12061 ctggaaaagg cctatcagga gtatctggaa ctgaagagag caaatttatg gaaggttcag
    12121 ccacaggctc agggaaaata tggtaaactg gtataaaaat ctgtacgtcg gggatactgc
    12181 aaaaaagaaa cagaaaaaat ggatgcgccg gattgagaac aaaaaagcag ttccgggagt
    12241 ctggctcctc acactgcctt ccaatcagca gaacaatctg gatgtaattc cggcggatct
    12301 gcttttgcag ccggccgtgc ggagaaactg tccgatgatt gtcggacttg ccgtgtcgcg
    12361 cgaggaagct tttgagcttg tggaggcgat cgcattggaa acttatcggg aaaccggaga
    12421 agtaaaaatc cgggaatggc tgctgaaccg gattgaggga tgtgaatgat acatatacta
    12481 ttcatgattt tgaaggcact ggggatttta ctccttgttt tactgtttct cgtgcttctt
    12541 atcgtctgca ccgtcctgtt tcttcctttt tgctaccggg cgcaggttct gaaagaagaa
    12601 gagggatttg cctgcgtaaa agcgagcgga agagtatcgt ggcttttcgg agctgtcgcg
    12661 ctgacggcct cgtatgagga acagaagccg gaggcgcaga ttctgctttt cggggcatca
    12721 ctggaaacat ggaaacggcg gctgaaaaag atccgccggg gagaagcgtc cgtgtcccgg
    12781 acggaggaaa atgagacaga aaatgcgccg gaagcagaaa aaacagcaga gcagaaagcg
    12841 gaagcgaaaa aagagcagac atcggcggaa caaccggcag aacaaaaaac agatactgta
    12901 aaaaaacaaa tgccgcaaag agacgcagaa aagacacaga aagcaccgga ccagacagaa
    12961 aaacagcaga aggtgacagc acagaaggag cagccagagc aagagcagga accggatgcg
    13021 ccgaaaaaaa gcattctgga acggtttttc ggaaggatcg aatatcttcc ggaaaaactg
    13081 ctgaatcttg tcagccgtct gctgcagaca gctttccggc tgttggaact tccgttccgt
    13141 ctgcttgaaa aactggagca gaaaatccag gcaggcagac ggttaaaaag aaagtgggag
    13201 agtgtgaaaa aattcttccg ttcgaagatg ttccgtgagg cgctgctcca tgcaaagaaa
    13261 gaagtgcttt atttcctgaa gaaagcagca ccgaaaaaag taaccggcac agtccggttc
    13321 ggatttgacg atccggcact gaccggagag acactcggca ttctgggtat gatttatgga
    13381 aaactgccga aagacctttc catccagccg gatttcgaac aggaaattct ccggggcgat
    13441 gtccggatga aaggaagttt tcaggctgtg acagcagccg gcattgcact tcgattattc
    13501 cgggatcaga atcttcggaa aaccatacga catttcaaac acaaggaggc ctaaaagatg
    13561 gcagaaaagg acaacacatt tcagaatact gtagaagcac tgttccgcgg aatggacagc
    13621 tttatcacaa caaagacggt cgtaggtgag gcgatccaca ttgatgatac gattcttctg
    13681 ccgctggtag acgtttcctt cggcgtggga gcgggtgcat tcgcagaaaa tgcaaagaac
    13741 aatgcaggag gcggtatggg cggaaaaatt tctccgagcg ccgttcttgt catcagcaaa
    13801 aacggaacgc gtctggtcaa catcaaaaac caggatgctg tcaccaaaat cattg
//
LOCUS       DAOMUF010000037        13783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_985_length_13783_cov_4.218531, whole genome shotgun sequence.
ACCESSION   DAOMUF010000037 DAOMUF010000000
VERSION     DAOMUF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_13783_cov_4.218531"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1056
                     /locus_tag="PLN77_03685"
     CDS             166..1056
                     /locus_tag="PLN77_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM43418.1"
                     /translation="MDINYELYKVFYYVATTLSFSEASKLLFISQSAVSQSIKVLEKK
                     LGITLFIRSTKRVQLTPEGETLLRHVEPAINLIARGEAQIMEAGTLGGGQLRIGASDT
                     ICRYFLVPYLNRFHRSFPNVHIKVTNQTSTQCVDLLESGQVDFIVTNYPNSHLSSRLH
                     TIPIHSFHDIFIANGEYYPELENRPVHLKELLQYPILMLDRKSTTSEFLHSQFQRFQL
                     DLVPEIELGSNDLLIDLASIGLGIAFVPDYCLVKETAAFQLDLVEELPPRQLVVAHSS
                     HLPIPQAAKEFMSMLEKGAE"
     gene            complement(1150..2727)
                     /locus_tag="PLN77_03690"
     CDS             complement(1150..2727)
                     /locus_tag="PLN77_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRM43419.1"
                     /translation="MEFVFEHATMIAVIAVVVIILAILASGYVKAPPDTAFIISGFQK
                     GQRILIGKAGIKLPFFERLDKLSLKLVAIDVKTSDAVPTADYINIQVDAAVNIKVGDE
                     PQKLQKASQNFLNRDSEYIAKVAREVLEGNMREIVGKMRLEEMVSDRQKFAELVKENA
                     EPDLAAMGLDIISFNVQNFVDSNQVIENLGIDNIVKIKKSAAIARANSEKEIKIAQAR
                     ANKEANDERVLAETEIAKKNNDLAIKSAELKKESDIKKAEADAAYKIQEEQQRKELEI
                     TSANANIARQEREVELRAREVEVTEKSLEAEVKKKAEAERYAKQQQAEADLYRRQKDA
                     EADQFEKIREAEAKKAQAEAQRYAMEQEALGIKAKGEAEAASIRAQALAEAEGMEKKA
                     EAYQRYNKAAMAEMMIKVLPEIAGKIAEPLSQIDKITIIGGGSEDNGIGSVAGNVPVV
                     MAKLFESMKEATGVDLSEIMRADTYDAKVNRNIQISSPEELKEYLKAEAGKAAADAES
                     PEKAEASEPAQKDGSEQ"
     gene            complement(2975..3322)
                     /locus_tag="PLN77_03695"
     CDS             complement(2975..3322)
                     /locus_tag="PLN77_03695"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRM43420.1"
                     /translation="MFGSKEASEDKLKKMVEKGKWDKLRKQYLDSDKTTQVALAKACA
                     ASRNDGSVNILTSLLEVDDVDVKIAAVTSLGEVGDDHVTALIRQLAVKTPADQTELKA
                     AITKALEKIVERA"
     gene            complement(3558..4328)
                     /gene="pflA"
                     /locus_tag="PLN77_03700"
     CDS             complement(3558..4328)
                     /gene="pflA"
                     /locus_tag="PLN77_03700"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRM43421.1"
                     /translation="MNQNNNGPVKGAIHSIESFGSVDGPGVRYVIFVKGCNMRCQFCH
                     NPDTWTTKDADWQTADEVLKKALRYKNYWGEKGGITVSGGEPLLQIDFLIELFRKAKK
                     NGVHTTLDTSGNPFTREEPYFSKFNELMKYTDLLLLDIKLIDDKAHRELTGCTNENIL
                     DLARYLDEIGKPVWIRHVLVPERSDYDEYLIRLDEFLHSLHNVQRVEVLPYHTLGVFK
                     WETLGIPYKLEGINPPTKERVENANKLLHTAEYTGYLK"
     gene            4589..4906
                     /locus_tag="PLN77_03705"
     CDS             4589..4906
                     /locus_tag="PLN77_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRM43422.1"
                     /translation="MNPQLKKGVLELCVLSQLTKEDKYGYELTDAISREMSIAAGTLY
                     MILKRLKEAGYVETYLQESASGPARKYYHLTEAGTAYQQEKKQEWLDFTQQINRLISD
                     ETI"
     gene            4924..5979
                     /locus_tag="PLN77_03710"
     CDS             4924..5979
                     /locus_tag="PLN77_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HRM43423.1"
                     /translation="MMNTTYETYMNALKQGLASFDSAAAEEILNDFESHFADARSQGL
                     SDEEICAELGNVDDVLEFFRQEYATTKQPVANVLPQEKTHSNSEETHPNYPYAPSTAI
                     TAMEISLRNASADFAPGTSASVEFDFSGDFDPDRFEAGIEGNTFCLREKKLIPPVFWK
                     HLFCNNHQKEVFSFQVPSTVQKLTLRSLNGATGLDTLSLTTLEISATNGKITGDSLSA
                     SSCRIQAANGKIVLTRLRTDSLDVSATNGKLEITGDCSRASLNSVNGKVLFEGTCSEY
                     LTAKSVNGSVKLHLPHDLADASIHASTTTGKIRLVSNGRTESYTKTFTRSGISAFTIQ
                     ASTVNGNVLLSDLPAEN"
     gene            complement(6073..7995)
                     /locus_tag="PLN77_03715"
     CDS             complement(6073..7995)
                     /locus_tag="PLN77_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM43424.1"
                     /translation="MMKNNYIKQKRKDQNPVNHWKIFEGQLVFLLAMVILFVVAYTFI
                     LQQAYTKTALKTEIERDISSADAVHKLVNDRLGRKDFNEIKSKADENTELFKNMSTYM
                     NEIRTLNSTRYIYTATRNEDGRLIYVVDGLDPSAGDVRHPGDPIEKEMVPYIEKALSG
                     KTVYSQDIVDTTWGPIFTACYPVTAEDESNEVIGAFCIEMDMQKAYGMVEKTNKLSIV
                     LGCITACILVILCTCGYSVYKKQKENEQKQQKLLQEAARLADSANKAKSTFLFNMSHD
                     IRTPMNAIIGYAELGEKHLKEPTILEDYFEKILLCGKKMLHLIDNILELARIENNQAT
                     LDETITDVSKNFDLCIDMFRKPIEEKHQTLKVNKNIRYPYLHMDDARSSEITINILSN
                     AVKYTGEGGNISCTLNQYPGEKEGWSVLELIIADNGIGMSEEFQKHIFESFSQERSSS
                     DSGIEGSGLGMGIVKKLVDLMNGKITVQSKPGAGSTFTITLPLKIANAEERNPKHADG
                     QLNIKKLEGKRVLLVEDNDLNAEIATELLTEEGIMIERAENGVACIDMFNKAKQNYYS
                     LILMDIQMPIMNGYEASRRIRELKDGNKSQIPIVAMTANAFEEDKKMALASGMNDHVA
                     KPIDMNVLLPTIMKYM"
     gene            complement(8195..11143)
                     /locus_tag="PLN77_03720"
     CDS             complement(8195..11143)
                     /locus_tag="PLN77_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM43425.1"
                     /translation="MDVKNYNRNRKKPVRKKGLRIVAFLLLFIGVVLAVFRYYKFMSK
                     SIYEESVSHLTEVLHQSDQMLRELTNKNLTYLHIWGENLQNISGEDEIRDYIKKAQED
                     AGFLEFFFLSSDGDYKMATGETGYLGLQENIEEDIRQGNDVIANAAVPGKSQLLVFAT
                     PKAHGIYQGFEYDAIAIAYENSDIVDVLDISAFDGNAQSFIIHPDGRVVIDHSSELWG
                     NVYNFFGFLSRHSDMSEKEINVLLEKFKAGRTDAMLLNLDGRNYYLVYEKSDIQDWMF
                     LGLVQADIVNASMNNLQFTTMLLVGAVVLCIAAFFISLIIQKNRKNLRRKDVEIRYRD
                     ELFQKLSMNVDDVFLMLDAQTYQTDYVSPNAEKLLGITVEQIRKDIRVLRKLHPADSE
                     DSQKNHLKEIPVHEQQEWDCEFIHRKTGERRWFHNIAMGSEVNGEKKYILVMSDRTSD
                     RKMNQALSEAVHAAETANRAKSIFLSNMSHDIRTPMNAIIGFTTLAASNIDDKKRVQD
                     YLGKILSSSKHLLSLINDILDMSRIESGKIHLEETEVCLSDVLHDLKTIISGQIHAKQ
                     LELYMDAMDVTNEDVYCDKLRLNQILLNLLSNAVKFTPAGGTVSVRLRQYPGKVKGSE
                     LYEFRVKDNGIGMSQEFVQKIFSPFERERTSTVSRTQGTGLGMAITKNIVDMMGGTIE
                     VQTEQDKGTEFIVRLPFRTQPEHHRIEKISELEGLKALVVDDDFNTCDSVTKMLVKVG
                     MRSEWTVSGKEAVLRARQSMELGDAFHAYIIDWRLPDMNGIEVTRQIRSLDDNTPIII
                     LTAYDWSDIETEARTAGVTAFCAKPLFMSDIRETLMAAIGQKQAQAEDKILPAADLDF
                     RGRRILLVEDNELNSEIAVEILKEYGFLVDTAENGAEAVEKIKNSTPGDYDLVLMDVQ
                     MPVMNGYEATKQIRALTDPALSGITILAMTANAFDEDRKKVLECGMDGFLSKPIIIEE
                     LIDTLQKNLD"
     gene            complement(11149..11889)
                     /locus_tag="PLN77_03725"
     CDS             complement(11149..11889)
                     /locus_tag="PLN77_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43426.1"
                     /translation="MIEENHMYIRIASSDFFSVSKDVVSKMISGEYDAGQAYQSFNSQ
                     LLEEETASEDIVLDSQKSYSNRFHSSGGNAAYSVMANTLRGIYGSDVLIATGNSFTGN
                     VLKAGYTEKMAGDMIMPNELSAYSSKMSGAELKEIIKNFVEGYEGGFIPFNRGSLPVV
                     SGISVEINETEDGYTLSKVTKDGKAVRDEDTFTVTCLATPQHMETYPVDENMIFDGGA
                     TSVKDTWTAYVSGGNAVLAEPEDYITLR"
     gene            complement(11846..12988)
                     /locus_tag="PLN77_03730"
     CDS             complement(11846..12988)
                     /locus_tag="PLN77_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43427.1"
                     /translation="MKKKKWSKVLSVFLVMVTAVSLLSGCGGKSAEKEDAETITVYLW
                     STNLYEKYAPYIQEQLPDINVEFVVGNNDLDFYKFLDENGGLPDIITCCRFSLHDASP
                     LKDSLMDLSTTNAAGAVYDTYLSNFRNEDGSVNWLPVCADAHGFVVNKDLFEKYDIPL
                     PTDYESFVSACQAFDKVGVRGFTADYSYDYTCMETLQGLSISELSSVEGRKWRTVYSD
                     SENTKREGLDDTVWPEAFERMEQFIQDTGLSQEDLNMNYDDVVEMYKSGKLAMYFGSS
                     FGVKMFQDQGINTTFLPFFQENGEKWIMTTPYFQVALNRDLTKDETRRKKAMEVLDTM
                     FSEDAQNRIISDGQDLLSYSQEVDMQLTEYKTCDRRKPYVYPYCVK"
     regulatory      complement(13076..13151)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            13463..13648
                     /locus_tag="PLN77_03735"
     CDS             13463..13648
                     /locus_tag="PLN77_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43428.1"
                     /translation="MDAKLIEELRQKYSKNPPEGMTSNLVKNMTDSDLLDMHYFLTED
                     DDLDDDEFEEGFYIDFF"
BASE COUNT         3406 a         3520 c         2636 g         4221 t
ORIGIN      
        1 gccaaagtgc ttggctgatt cccctttctt atctgccgga accgtgaatt ttctcttact
       61 cgaaaaagag gagaaaaagt tcacggctcc ggcagatttt ttcttgcatt ctttcgggga
      121 agtgtgctag agtgaaggta tcatatttta taagaggtgc ttattttgga tattaattac
      181 gagttatata aggttttcta ttacgttgcc accacactca gtttttccga ggcctccaag
      241 ctgcttttta tctcgcagtc ggcggtcagc cagtcgatca aggtactgga gaaaaagctt
      301 ggaatcacgc tctttatccg cagtacaaag cgcgtgcagt taacaccgga gggagaaacg
      361 ctgctgcgcc acgtagagcc tgcgatcaac ctgattgcgc gcggtgaagc acagattatg
      421 gaagcgggaa cgcttggcgg cggccagctc cgcatcggcg cgagcgacac aatctgccgc
      481 tatttccttg ttccgtacct gaaccgtttc caccgctcct ttccgaatgt ccacatcaag
      541 gtgacaaacc agacttcgac ccagtgtgtc gatctgctgg aatccggcca ggtggatttc
      601 atcgtgacca attatccaaa ctcgcatctt tccagccgtc ttcacacgat tccgattcat
      661 tcgttccacg atatcttcat cgcgaacggc gaatattacc cggaactgga aaacaggccg
      721 gttcacttaa aagagctgct gcagtacccg attctgatgc tcgaccgcaa gagtacgacg
      781 agtgagttcc tgcacagcca gtttcagcgg ttccagctcg acctcgtgcc ggaaattgag
      841 cttggcagca acgatcttct gatcgacctg gcgagtatcg gccttggcat cgcgtttgtg
      901 ccggactact gtcttgtaaa agagacggcg gccttccagc tcgacctcgt ggaggagctt
      961 ccgccgcgcc agcttgtcgt tgcccacagc tcccatctgc cgatcccgca ggcggcaaaa
     1021 gagttcatga gcatgctgga aaaaggcgcc gaataacttt ttccgatcta tacgtcaaaa
     1081 gagacagaat cctcaagaaa tttttctgga tccctgcctc tttttctgtt tttatgatcc
     1141 gttctggatt tactgctcgc tgccgtcttt ctgcgccggt tcggatgctt ccgctttttc
     1201 cgggctttct gcatccgctg ctgcctttcc ggcttctgct ttcagatact ctttgagctc
     1261 ttccgggctg gagatctgaa tgttgcggtt tacttttgca tcgtacgtat cggctctcat
     1321 gatttcggag agatcgacac cggtcgcctc tttcatcgac tcaaacagct ttgccatcac
     1381 caccggcaca ttgcccgcta cggagccgat gccgttgtcc tcgcttccgc cgccgatgat
     1441 cgtaatcttg tcgatctggc tgagcggctc tgcgatcttt ccggcgatct ccggcagaac
     1501 cttgatcatc atctctgcca ttgcggcttt gttgtagcgc tggtaagcct cggctttctt
     1561 ctccatacct tcggcctctg caagtgcctg ggcgcggatg gatgcggctt ccgcctcacc
     1621 ttttgccttg ataccgagtg cttcctgctc catcgcatag cgctgggctt ctgcctgggc
     1681 tttctttgcc tccgcctcgc ggattttttc gaactggtct gcctctgcgt ctttctgacg
     1741 gcggtacaaa tctgcctctg cctgctgctg ttttgcgtaa cgctcggcct cggctttctt
     1801 cttgacttct gcctcgaggg atttctcggt tacttctact tctctggcac ggagctcaac
     1861 ctcacgctcc tgacgggcga tgttcgcatt cgcggaagtg atttccagct cttttctctg
     1921 ctgctcttcc tgaatcttgt acgctgcatc tgcctctgcc tttttgatat cggactcttt
     1981 tttcagctct gccgacttga ttgccagatc gttgtttttc tttgcgatct cggtctcggc
     2041 aagaacgcgc tcatcgttcg cctctttatt tgcacgcgcc tgggcaattt tgatttcttt
     2101 ctcggaattg gcacgggcga tcgccgcgga tttcttgatt tttacgatat tgtcgatacc
     2161 gaggttttca atgacctgat tggaatcgac gaaattctgt acgttgaagc tgatgatgtc
     2221 aagtcccatc gcagccaggt ccggttctgc attttctttg accagctccg caaacttctg
     2281 acggtcggaa accatctctt ccagacgcat ctttccaacg atctcacgca tgtttccttc
     2341 aaggacttct cttgcaacct tggcaatata ttcgctgtcg cggttcagga agttctggga
     2401 tgctttctgg agcttctgcg gttcgtcccc gactttgatg ttgacggctg catccacctg
     2461 aatattgatg tagtcagcgg tcggcacggc gtcgctcgtc ttcacatcga tcgctaccag
     2521 cttcagactc agcttgtcca gccgctcaaa gaatgggagc ttgatgccgg ctttaccgat
     2581 caggatccgc tggccttttt ggaaaccgga aataataaat gcggtatccg gcggcgcctt
     2641 cacataaccg ctcgccagaa tggcaagaat gatcaccaca acggcgatca cggcaatcat
     2701 ggttgcatgt tcgaatacaa attccatctt gtttcctccc ttttttctgc ggatgctttt
     2761 cgctccgcgt tctgcttttg tgtctgtgtt attttcacta tagcattttt tgcgggatag
     2821 atacaactgc attattatta cgattttgta agatttctgt tcgggcaaaa acaatccgct
     2881 ggagcagatt ctgatatgcc ttgcaacaaa aaaacgaagg agcttctttt tacagaaact
     2941 tcttcgcctt tccttttttt cactgatcct gctcttatgc tctttcgaca atcttctcca
     3001 gcgcttttgt gatcgctgct ttcagctccg tctgatctgc cggtgtcttg acagccagct
     3061 gacggatcag tgctgttacg tgatcgtctc caacctcacc aaggctcgta acggccgcga
     3121 tttttacgtc cacatcgtct acttccagaa gagaagtcag aatattaaca gaaccatcat
     3181 ttctcgaagc tgcacaggct tttgcaagcg ctacctgcgt tgtcttgtcc gaatccagat
     3241 actgttttct taatttgtcc cattttcctt tttccaccat ttttttcagc ttgtcttccg
     3301 atgcctcttt tgaaccaaac attgtaaatc cttctttctt tttgctttta tttttggtgg
     3361 atgcagcaac tgccgcggcg aatcaaaaag gggcgtagtg tttttgctgt cgtccctgtc
     3421 atgtccgtca cattgtaacc atgctctcgt ccataacaga gattatactc cttttcttct
     3481 ggtttttgaa cagaaatact ataaaatttt tataaaaatt gttacttttt cacagatttt
     3541 tcttgttgtt ctgcttttta tttcagatat cccgtatatt ccgccgtatg cagcagttta
     3601 ttggcgtttt ccacgcgctc tttggtcggc gggttgatgc cttcgagttt gtatgggatt
     3661 cctaaggttt cccatttgaa tacgccgagg gtgtggtatg ggagaacctc tacgcgctgt
     3721 acgttgtgca ggctgtgtaa aaactcatcg aggcgaatca ggtattcgtc gtagtcgctg
     3781 cgttccggga cgaggacgtg gcggatccag actggtttgc cgatctcgtc aaggtagcgc
     3841 gccaggtcga ggatgttctc gttggtgcag ccggtcagct ctctgtgcgc cttgtcgtca
     3901 atcaacttga tatcgagcag cagcaggtcg gtgtatttca tcaactcatt aaatttgctg
     3961 aaatacggct cctctcttgt gaacggattt ccactggtat ccagcgttgt gtgaacgccg
     4021 ttcttttttg ccttgcggaa cagctcgatc aggaagtcga tctgcagcag cggttctccg
     4081 ccgctcacgg taatgccgcc tttttctccc cagtagtttt tatagcggag cgcttttttc
     4141 agcacctcgt ctgcggtctg ccagtcggca tcttttgttg tccaggtgtc cgggttgtgg
     4201 cagaactggc agcgcatgtt gcagcccttc acaaaaatca cgtagcgtac gccaggtccg
     4261 tcgacggaac cgaagctctc gatcgaatgg atcgcgccct ttaccgggcc attgttgttc
     4321 tgattcattt cactcatgtt ttttcctctt ctttccaact aaccatcttt gcatttatct
     4381 gtattttccg gtatttttcc atttcctgga tgcccggaaa attcctgccg tttttcaggg
     4441 ttctgcttga ttatacggaa atccgcggtt cttttcaagg catttccatg tacaaaatcg
     4501 aacacatttt tctttgtact tttttctttt actatattgt attatgatat agtctgtgtt
     4561 atgatctact caacaaggag gtgttcatat gaatccacaa ctcaaaaagg gcgtcctcga
     4621 gctctgtgtc ctctcccagc tgacaaaaga ggacaaatac ggctatgagc tgacagacgc
     4681 catttcccgc gaaatgtcca tcgccgcagg aacgctctat atgattttaa aacgactcaa
     4741 ggaagccggt tacgtggaaa cgtatctgca ggaatccgca tccggccctg cccggaaata
     4801 ttaccatctg actgaagcag gaacagctta tcagcaggag aaaaaacagg aatggctgga
     4861 tttcacccag cagatcaacc gactgatttc agatgaaacg atctgacgat agaaaggaaa
     4921 atcatgatga atacaacgta tgaaacttat atgaatgcat tgaaacaggg ccttgcatcc
     4981 tttgattctg ccgccgcaga ggaaatttta aacgatttcg aatcccattt cgccgacgcc
     5041 cgcagccagg gtctctccga tgaggaaatc tgtgcggaac tcggtaatgt cgacgatgtc
     5101 ctcgaatttt tccgtcagga atatgccaca acgaagcagc ctgttgcaaa cgtgctgcca
     5161 caggagaaaa cccactccaa ttctgaagaa acacatccga actacccgta cgccccttcc
     5221 accgccatca ccgctatgga aatctccctg cggaacgctt ctgccgactt tgctccgggc
     5281 acttccgctt cggttgagtt tgacttttcc ggcgacttcg atccggaccg cttcgaggca
     5341 ggcattgagg gcaatacgtt ctgtctgcga gagaaaaaac tcatccctcc cgtattctgg
     5401 aaacatcttt tctgcaataa tcaccagaaa gaggtgttct ccttccaggt gccgtccacc
     5461 gtacagaagc tgacgctccg ctctttgaac ggcgcgaccg ggcttgatac cctctccctt
     5521 actacgctcg aaatttccgc cacaaacggc aaaatcaccg gtgattccct ttccgcatcc
     5581 agctgccgca tacaggctgc gaacggaaag atcgtcctga cccgtctgcg cacagacagc
     5641 ctcgatgttt ccgccacaaa cggcaaactt gaaatcaccg gcgactgctc ccgcgcatcc
     5701 ttaaacagtg tcaacggtaa agtcctcttc gagggaacct gttccgagta cctgacagca
     5761 aaatccgtca acggttccgt gaaactccat ctcccgcacg accttgccga tgcgtccatt
     5821 catgcatcca cgacaaccgg aaaaatccgc ctcgtctcca atggaagaac ggaatcctac
     5881 acaaaaacat tcacccgctc cggtatttct gcattcacca ttcaggcaag taccgtcaac
     5941 ggaaatgtcc tgctctctga cttgccggca gaaaactgac aaaaaagaat ggactggata
     6001 aaatgttttt ctttagtcga ctgatatgta agaagaacac tgactacaaa atcagtgtcc
     6061 ttctttcttc ctttacatat atttcataat agtcggcaat aatacattca tatctattgg
     6121 ttttgcaaca tgatcattca tgccacttgc caaagccatc ttcttatctt cttcaaacgc
     6181 atttgctgtc attgcaacaa ttggaatctg cgatttgtta ccgtctttca gttctcgaat
     6241 tctcctggat gcttcatatc cgttcataat cggcatctga atatccatca gaatcagact
     6301 gtagtaattt tgctttgctt tgttaaacat atcaatacat gcaacgccgt tctccgctct
     6361 ttctatcatg attccctctt ccgtcaaaag ttccgttgca atttctgcgt tcaagtcatt
     6421 atcttctacc agaagaaccc tttttccttc cagttttttg atattcaact gaccgtctgc
     6481 atgtttcgga ttcctttctt ccgcatttgc tatttttaat ggaagcgtga ttgtaaaagt
     6541 agaacctgct cccggtttgc tctgaactgt gatcttgcca ttcattaaat ccaccagttt
     6601 cttcacgatt cccatgccaa gtccgctacc ttctattccg ctgtctgacg aagatcgttc
     6661 ctgtgaaaat gattcaaaaa tatgtttttg gaattcttct gacattccga ttccgttgtc
     6721 tgctatgata agctctaata cactccaacc ttctttttcg ccgggatatt ggttcaatgt
     6781 acagcttatg ttaccgcctt cacccgtgta tttcacagca ttactcaaaa tgttaatcgt
     6841 aatttcagat gatcttgcat catccatatg caggtatgga tatcgaatgt tcttattgac
     6901 tttcagtgtt tgatgttttt cttctatcgg tttcctaaac atatcaatac ataaatcaaa
     6961 gtttttagaa acatctgtta ttgtttcatc cagcgttgct tggttgtttt cgattcgtgc
     7021 taattcaaga atgttatcaa tcaaatgcag cattttcttt ccgcacaata atattttttc
     7081 aaagtaatcc tctaatatag ttggctcctt taaatgtttc tctcccaatt ctgcatatcc
     7141 aatgattgca ttcatagggg tacgaatatc atgagacata ttaaatagaa atgttgattt
     7201 tgctttatta gctgaatcgg ccagtcttgc agcttcctgc aacagttttt gctgtttctg
     7261 ctcattttct ttttgcttct tatatacaga atatccacag gtacataata ttaccaaaat
     7321 acacgctgta atgcatccca gtacaatact taacttattc gtcttctcaa ccattccata
     7381 cgctttctgc atatccattt ctatgcagaa agcacctatc acttcattag attcatcttc
     7441 cgctgtcaca ggataacagg ctgtaaaaat aggtccccat gttgtatcaa caatatcctg
     7501 agaatatact gtttttccag aaagtgcttt ttctatataa ggaaccatct ccttttcaat
     7561 cggatctcct ggatgtctga cgtcaccagc cgatggatcc agtccgtcta caacgtaaat
     7621 caatctaccg tcttcatttc tggttgctgt gtaaatatat ctcgtagaat tcagcgttct
     7681 gatttcattc atatatgtcg acatattctt gaatagttct gtattctcat cagctttact
     7741 ttttatttca ttaaaatctt ttcgtcccag tctgtcattt accagcttat gaaccgcatc
     7801 tgcactggaa atatctcgtt caatttctgt tttcagagca gttttggtat atgcttgttg
     7861 taaaataaaa gtatatgcaa caacaaacaa aatcaccatt gccagaagga ataccaactg
     7921 tccctcaaaa attttccaat gatttaccgg attttgatct tttctttttt gttttatata
     7981 gttatttttc atcatcgtat ttcacatgcc ttttatctaa ttttaattta atacaaaaac
     8041 gtctcaactc ttcacgtatt ttgcaaacat gggttacatt tccagactca tccctttttt
     8101 acagtaaaaa tttttccatg cacctggaag gctcctgatt gcctgatttc catttccctc
     8161 ttctaccttg tgggttttat gatgttccag gcacctaatc cagattcttt tgtaacgtat
     8221 cgatcaattc ttcaataatt attggcttgg ataagaatcc atccataccg cattccagaa
     8281 ctttctttct gtcctcatca aaagcattag cagtcatggc aagaatggta attcccgaca
     8341 gtgccggatc agtaagcgca cggatctgct tggtagcctc atatccattc attacaggca
     8401 tctgcacatc catcagtacc agatcataat cccctggtgt tgaattttta attttctcca
     8461 ctgcctctgc tccattttct gctgtatcaa ccaggaatcc atattctttc aaaatttcca
     8521 ctgcaatttc actgttcagt tcattatctt ccacaagaag tatacgcctg cctctgaaat
     8581 ctaaatctgc tgccggaaga atcttatcct ctgcctgggc ctgtttctga ccgatggcag
     8641 ccatcagagt ctctctgata tctgacataa acagaggttt cgcgcagaat gcagtcactc
     8701 ctgcagttct ggcttctgtt tcgatatctg accagtcgta tgcagtcaga ataatgatcg
     8761 gcgtattgtc atcaagactg cggatctgac gtgtaacttc aattccattc atatccggca
     8821 gacgccaatc gatgatataa gcatgaaatg catcccccag ctccattgac tgtcgtgcac
     8881 ggagaacagc ctctttaccc gagactgtcc attccgaacg cattccaacc tttacaagca
     8941 tctttgttac actgtcacag gtgttaaagt catcgtctac aaccaatgcc ttaagaccct
     9001 ctagttccga aatcttctct atgcgatgat gctcaggctg agtccgaaat ggcagacgga
     9061 ctataaactc ggtgcctttg tcctgctctg tctgaacctc aatagttccg cccatcatat
     9121 ccacgatgtt cttggtaatg gccattccaa gtcccgtacc ctgagtcctg ctgactgtag
     9181 aagtccgctc ccgctcaaaa ggagaaaaga tcttctgtac aaattcctga ctcattccaa
     9241 ttccgttatc ctttacccga aattcataca gttcgctgcc ttttactttt cccgggtact
     9301 gtctgagacg gacagaaacg gttccgcctg ccggagtaaa cttaaccgca tttgacaaaa
     9361 gattcagcag tatctggttc agccgcagtt tgtcacaata gacatcctca ttcgtaacat
     9421 ccatggcatc catataaagc tccagctgct tcgcatgaat ctgtccactg atgatcgtct
     9481 tcagatcatg aagcacatcc gacagacaga cttctgtttc ttccagatgg atctttccgc
     9541 tctcaatccg gctcatatcc aatatatcat tgatcagtga aagcagatgc ttactggaag
     9601 aaagaatttt gcccagataa tcctgaaccc tttttttgtc atcaatatta cttgcagcca
     9661 atgttgtaaa tccaataatc gcattcattg gtgtccggat atcatgagac atattggaaa
     9721 gaaaaatact cttcgcccta tttgcagtct ctgccgcatg gactgcctca gaaagagcct
     9781 ggttcatttt ccggtcagag gtacggtctg acataaccag aatatatttc ttttccccgt
     9841 ttacctcact tcccattgca atattatgaa accagcgcct ttcccctgtc ttccgatgta
     9901 taaattcaca atcccactcc tgctgttcat gaaccggaat ttctttcaga tgattcttct
     9961 gcgagtcttc agaatctgca gggtgcagtt ttcttaaaac acgaatatcc ttgcgaatct
    10021 gttctactgt aatacccagc agtttttccg cattagggct gacgtaatcc gtctgatatg
    10081 tttgggcatc cagcatcagg aaaacgtcat ccacattcat ggaaagcttt tgaaacagct
    10141 catcacggta ccggatctcc acatcctttc ttctgagatt tttcctgttt ttctggataa
    10201 taagactgat aaaaaaggca gcaatgcaaa gtacaacagc tccaacaaga agcatcgtgg
    10261 taaactgcag attgttcata ctggcattga caatatcagc ctggacaagt cccagaaaca
    10321 tccagtcctg aatatccgat ttttcataga ccagataata atttcttcca tccagattca
    10381 gcaacatcgc atcagtacgt cccgctttaa acttctccaa aaggacattg atctcttttt
    10441 cagacatatc agaatgccta ctcagaaaac cgaagaaatt atacacgttc ccccataact
    10501 cggaagaatg atcaatcaca acacgaccat ccggatgaat aataaaactc tgggcatttc
    10561 catcaaaagc agaaatatcc agtacatcta cgatatcaga gttttcataa gcaatggcaa
    10621 tggcatcata ttcaaatccc tgataaattc catgcgcctt aggtgtggca aaaacaagca
    10681 gctgggattt tccgggaact gctgcattcg ctatcacatc atttccctgc cggatatcct
    10741 cttcaatatt ttcctgtaat ccaagatacc cggtttcacc tgtcgccatc ttgtagtcac
    10801 cgtcagatga tagaaaaaag aattccaaaa agcctgcatc ttcctgcgct ttctttatat
    10861 aatcacggat ctcgtcttca ccggaaatat tctgcagatt ctcgccccaa atatggaggt
    10921 aagtcaggtt cttatttgtc agttctctca gcatctgatc agactgatga agaacttccg
    10981 tcagatgaga aacactttcc tcgtaaatag atttcgacat aaacttataa tatcggaaaa
    11041 cagccagaac aactcctata aagagaagaa gaaaagcaac tattcttaat ccttttttcc
    11101 tgacaggttt ctttctattt ctattataat tcttaacatc catttttctc acctcagggt
    11161 tatgtaatct tcaggttctg caagaacggc attaccacct gaaacatatg ctgtccaggt
    11221 atctttcaca gatgtagctc ctccatcaaa tatcatattt tcatctacag gataagtttc
    11281 catatgctgc ggtgtggcaa gacaggtcac cgtgaaagta tcctcatccc gaactgcctt
    11341 tccatccttt gtaactttac ttaatgtata gccatcttcc gtttcattaa tttccacaga
    11401 aataccacta accactggca aagagccacg gttaaacgga ataaatcctc cctcatagcc
    11461 ttctacaaag tttttgatta tttctttcag ctcagcccca ctcatcttgc tgctgtaagc
    11521 tgaaagttcg ttcggcatga tcatatcgcc cgccattttt tcagtatagc cggctttcag
    11581 cacgtttccc gtaaagctgt ttccggttgc gatcagcaca tctgacccat agatgccacg
    11641 tagcgtgttt gccataacag aatatgctgc atttcctcca ctgctgtgga aacggttgga
    11701 atacgacttc tgtgaatcca gcacgatatc ctcagaagca gtttcttcct caagaagctg
    11761 agaattaaat gactggtatg cctgccctgc atcgtattct cctgagatca ttttggaaac
    11821 cacatccttg gaaacagaga aaaagtcact tgacgcaata cggatataca tatggttttc
    11881 ttcgatcaca ggttttatat tccgtaagct gcatatccac ctcctgactg tagcttaaca
    11941 gatcctgtcc atcactaatg atcctgttct gcgcatcctc tgaaaacatt gtatccagta
    12001 cctccattgc tttctttctg cgtgtttcat cctttgtcag atcacggttt aaggccacct
    12061 ggaaatatgg cgtagtcata atccactttt caccgttctc ctgaaagaat ggaagaaatg
    12121 ttgtgttaat gccctgatcc tggaacattt ttacaccgaa agaactgcca aagtacatgg
    12181 caagcttgcc acttttgtac atctcaacaa catcatcata attcatattc aaatcttcct
    12241 gactaagccc tgtatcctga ataaactgtt ccatacgttc aaaagcctct ggccagacgg
    12301 tatcgtccag accttctctc tttgtatttt ccgagtcact gtagaccgtc cgccacttgc
    12361 gtccttccac agaagacaac tctgatatag acagaccctg cagtgtttcc atacaggtat
    12421 aatcataaga atagtctgcc gtaaatccac gaacacccac tttgtcaaat gcctggcagg
    12481 cggaaacaaa actctcgtaa tctgttggca acgggatatc atatttttca aaaaggtctt
    12541 tgttcactac aaaaccatgg gcatctgcac acacaggaag ccagtttacg cttccatcct
    12601 cgttcctgaa attgctgagg taggtatcat agacagcacc tgccgcattg gttgtggaaa
    12661 gatccatcag actgtctttc aggggacttg catcatgcag ggaaaatctg cagcaggtta
    12721 taatatccgg caatccaccg ttttcatcaa ggaacttgta gaaatccaaa tcattattcc
    12781 ctacaacaaa ctcgacattg atatccggaa gctgttcctg gatatatggt gcatattttt
    12841 catacaggtt tgtgctccat aaatatactg tgattgtttc tgcatcttct ttttccgcac
    12901 tcttcccgcc acagcctgac agaagcgata cggctgtcac catcactaaa aatacagaca
    12961 aaaccttgct ccattttttc tttttcatta caagttctcc taatagaatt gttatagtca
    13021 cgtctatgaa ataacaccgt aaaaaagcac tgccaaacag acagtgcacc tcatttcatg
    13081 caactggctg ccattttaca ggccctgtag ctttgcgtcc cagactttca tctggtttgc
    13141 cgatcgtcct ttttaagctt ttaaacttat gcaaaaaact gattttatta tactatggca
    13201 ctctactgta cacaagaaaa acattaccat tttaaccttc aaaagcaaaa aaagaacagc
    13261 cgatacatat actcgaactg ttcttctact tccgaagtcc acgcagcatt gcttttagta
    13321 gaaatctatc acaatcataa acgagttcct cttcaagaac tttacgaaag ggccatggca
    13381 aaagctaaat gccgctctgc ttgatttttc tgcctgtctg tatcatactg aacttattaa
    13441 cagacaggga ggaatttaaa ccatggatgc aaagcttata gaggaactca gacaaaaata
    13501 cagcaagaat cctccagagg gaatgacatc caatctggtc aaaaacatga cagactctga
    13561 ccttttggat atgcactatt ttcttaccga ggatgatgac ctcgatgacg atgaatttga
    13621 agaaggtttt tacatcgact tcttttaatc tatcgtctgt tttctatgcc cgcttttgtg
    13681 cgggctcttt ttatcaagag ttcgacgaat gtcgaacttt cctattatat aaaaagaaaa
    13741 agccgctgac acgttatctt ctaacgcatc aacagcttct gtc
//
LOCUS       DAOMUF010000038        13689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_998_length_13689_cov_2.968828, whole genome shotgun sequence.
ACCESSION   DAOMUF010000038 DAOMUF010000000
VERSION     DAOMUF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 13689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13689
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_13689_cov_2.968828"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLN77_03740"
     CDS             complement(<1..431)
                     /locus_tag="PLN77_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRM43429.1"
                     /translation="MSVIVSLMGGLGFFLYGMKLMSEGLQKVAGSKMRSILEVFTKNR
                     VIGLLVGIFFTALIQSSNATTVMVVSFVNSGLMKLAQAPGIILGANIGTTVTGQLIAF
                     DLADIAPLFVIIGVLMVMFVKNNLTISRLGEVILGFGILFMG"
     gene            744..2159
                     /locus_tag="PLN77_03745"
     CDS             744..2159
                     /locus_tag="PLN77_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRM43430.1"
                     /translation="MMENTFKLRENGTTVKTEIIAGVTTFMTMAYILAVNPNILSSTG
                     MDHGAVFTATALAAFLGTLLMALFANYPFALAPGMGLNAYFAYTVVLGYGYSWQAALT
                     AVFAEGIIFIVLSLTNVREAIFNSIPASLKSAVSVGIGLFIAFIGLQNAKIVLPNSST
                     VAGLYSLSSYNANLQSAATLNGTEYVAGTFNDVGITVLLAVIGVIFTAVLVVRNVKGN
                     ILWGILATWILGMICQACGLYVPNPANGFYSLFPDFSNGLSIPSLAPIFGKLDFSILK
                     TGEFFVIMFAFLFVDMFDTIGTLIGVSSKANMLDKNGKLPRIKGALMADAVATCAGAV
                     LGTSTTTTFVESASGVSEGGRTGLTAVTTAILFGLALFLSPIFLAIPSFATAPALIVV
                     GFYMLTNVVNIDFSDLSEAIPCYICIGAMPFFYSISEGIAMGVISYVAIQILSGKAKE
                     KKISVIMYILAVLFILKYLLL"
     gene            complement(2261..2572)
                     /gene="hisI"
                     /locus_tag="PLN77_03750"
                     /pseudo
     CDS             complement(2261..2572)
                     /gene="hisI"
                     /locus_tag="PLN77_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            complement(2628..3218)
                     /gene="hisB"
                     /locus_tag="PLN77_03755"
     CDS             complement(2628..3218)
                     /gene="hisB"
                     /locus_tag="PLN77_03755"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRM43431.1"
                     /translation="MENRTASVERVTKETKIRMTLNLDGTGKSDIHTGIGFFDHMLDG
                     FARHGLFDLSVSVDGDLDVDGHHTIEDTGIVLGTAIREAVGDKQGIRRYGDRILPMDE
                     TLVLSAVDLCGRPYLSFQAEFTREKVGEMETEMVKEFFYAVSYAAQMNLHIKVLDGGN
                     NHHIIEAMFKAFAKALDEATSFDPRIAGVLSTKGSL"
     gene            complement(3219..4517)
                     /gene="hisD"
                     /locus_tag="PLN77_03760"
     CDS             complement(3219..4517)
                     /gene="hisD"
                     /locus_tag="PLN77_03760"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRM43432.1"
                     /translation="MRIVKLTKETTENILENMLKRSPTQYGTYEASVQEIIENVKTRK
                     DAAVFEYTEKFDHAVLDASTVEVTQEEIDEAYNIVDPELIGVIRRSMKNIREFHEKQK
                     QNSWFTSTENGTMLGQKLTALNRVGVYVPGGKAAYPSSVLMNILPAKVAGVKEICMTT
                     PCGRDGKVNPVVLAAAKEAGADRVFKIGGAQAIAAFAYGTESIPKVDKIVGPGNIYVA
                     LAKKAVYGNVSIDSIAGPSEILVLADETANPRFVAADLLSQAEHDELASAILITTSQE
                     LAEKVSEEVDGFLKVLSRSDIISKSLDNFGYILVTDSHQEAIDTVDAIAPEHLEIVTK
                     NPFEDMMKVHNAGAIFLGENSSEPLGDYFAGPNHILPTNGTAKFFSPLSVDDFVKKSS
                     IIYYSREALSEIHEDIESFAKSESLTAHANSIAVRFEGEE"
     gene            complement(4514..5152)
                     /gene="hisG"
                     /locus_tag="PLN77_03765"
     CDS             complement(4514..5152)
                     /gene="hisG"
                     /locus_tag="PLN77_03765"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRM43433.1"
                     /translation="MRYLTIALTKGRLAYQTLELLEKVGITCDEMKNKDTRKLIFTDE
                     EHKLRFFLAKGPDVPTYVEYGAADIGIVGKDTILEEGRRLYEVLDLGYGKCRMCVCGP
                     ESARELLQHNQQIRVATKYPNIAKDYFYNRKHQTVEMIKLNGSIELAPIVGLSEVIVD
                     IVETGSTLRENGLTVLEEVCPLSARMVVNQVSMRMENERITNLIQDLKGALV"
     gene            complement(5174..6415)
                     /gene="hisZ"
                     /locus_tag="PLN77_03770"
     CDS             complement(5174..6415)
                     /gene="hisZ"
                     /locus_tag="PLN77_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRM43434.1"
                     /translation="MDRLIHTPEGVRDVYNQECTKKLYLENQIEQVFHAFGYQDIETP
                     TFEFFDVFGREVGTTPSRELYKFFDRDGNTLVLRPDFTPSIARAASMYFMEETMPIRL
                     CYRGSSFVNSSSYQGRLKESTQMGVELLNDDSAMADAEIIAMTVKVMLDAGLTDFQIS
                     IGHVGFFQALAEEAALSEDVLNELKELISIKNHFGAEELLAKQNLRSDLFEALRDVPQ
                     LFGNAEILEKAKGLTRNPAAREALDRLATIYEIVKDYGYEKYVFFDLGLLSKFHYYTG
                     IIFQAYTYGTGEPIVKGGRYDALLQHFGKKAPAIGFCTVMESLMNALERQKISLPIVN
                     TKTMLLYPDFLQPLAVKLANEHRSKGMDVALVCFARDKVLADYEEYGRKNQFGGIVYI
                     QKSDSVLAINLSTGEVQPVEL"
     gene            complement(6523..7071)
                     /locus_tag="PLN77_03775"
     CDS             complement(6523..7071)
                     /locus_tag="PLN77_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HRM43435.1"
                     /translation="MMAWTSRGLRGSTLEEMINRTNEQYRDKHLALIQKVPTPITPIK
                     IDKESRHITLAYFEKKSTVDYIGAVQGIPVCFDAKECKTDSFPLANIHEHQVTFMKDF
                     EQQGGISFLLLYFTARDEYYYLRYETMKTFWDRGQNGGKKHFSYTELETSFFLPRTGS
                     SVLVPYLNGIQKDLEIRDSKAD"
     gene            complement(7097..>7524)
                     /gene="lepB"
                     /locus_tag="PLN77_03780"
     CDS             complement(7097..>7524)
                     /gene="lepB"
                     /locus_tag="PLN77_03780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM43436.1"
                     /translation="IPSPSMENTIMTGDQIFGNRLAYVKSDPQRYDIVIFYYPDDEKQ
                     KFIKRVIGLPGETVTIRDGKVYINDSTEPLRDDFCPETPVGDFGPYEVPEGCYFMLGD
                     NRNVSKDSRYWLNPYVEKDKIIGKAFLRYWPLNKISLIE"
     assembly_gap    7525..7534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7675..8661)
                     /locus_tag="PLN77_03785"
     CDS             complement(7675..8661)
                     /locus_tag="PLN77_03785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM43437.1"
                     /translation="MRELTVTEKDCAQQLIKYLSKYMEEAPKSFFYKMLRKKNIVLNQ
                     KKADGSEKLKTGDTIRLYLAEETIEKFRKTPEKPAAGKQVKYPKLDIVYEDEKVIFLN
                     KPAGVLSQKAKETDVSLTEALGAYLSEKNAGEETMFRAGLCNRLDRNTSGLILAGKTV
                     AATQQLSELIAERAVGKYYLTVVKGTVTETERIRGYLIKNERTNRVSIQREQQGDAVY
                     IETAYRPLCAGRGCTLLEVELVTGKSHQIRSHLASIGHPLAGDTKYGDAEFNRRFREK
                     YGLKYQLLHSWKAVFPELTGTLSDLSGKTFTAPLPSSVRRILQGEHLTGEGL"
     gene            complement(8674..9291)
                     /locus_tag="PLN77_03790"
     CDS             complement(8674..9291)
                     /locus_tag="PLN77_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 family protein"
                     /protein_id="HRM43438.1"
                     /translation="MKIRKGDYGYIRREKKKRLLVTLGLFVLPAIVFIVGLVLADGNK
                     SNIFTVVAVVGCLPGCRAAVGFIMMVMQKPVDKAVYDAIEAKKGKLLMGYEMYITQEK
                     SSLMIEAAAFCGEEIACYTSRAKDQKQIEDCTTYLNKIIRANGYKCHVKIFEREKAFL
                     ERLDSLNRNYDELEKSASENFKPDERYPDLSRTELVKHTMLALAL"
     gene            complement(9343..11263)
                     /locus_tag="PLN77_03795"
                     /pseudo
     CDS             complement(9343..11263)
                     /locus_tag="PLN77_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            complement(11267..12025)
                     /locus_tag="PLN77_03800"
     CDS             complement(11267..12025)
                     /locus_tag="PLN77_03800"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM43439.1"
                     /translation="MSKLRLDKYLADMGVGTRQEVKALIRKGRVAVDGITAKAPELKV
                     DPDEAQVTVDGNSISYVKMEYWMLHKPAGVVSATEDRRDRTVLDLLSDAARKDLFPVG
                     RLDKDTEGLLLITNDGALSHRLLSPKSHVSKVYEAVIDGKVTEEDRQAFAEGLDIGDD
                     KPTLPAELTILETTEKEPGIFESRIRITICEGRFHQIKRMFEKVGKTVVYLKRLSMGS
                     LKLDPALPKGSARLLTQEEKAALGIADAETQKGK"
     gene            complement(12022..13395)
                     /locus_tag="PLN77_03805"
     CDS             complement(12022..13395)
                     /locus_tag="PLN77_03805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRM43440.1"
                     /translation="MTLPEAFEQEMKQLLGEEEYEAYRKTFDEAVHRGLRVNNGKIST
                     EEFLRRTDIPLKKVPWIPNGFYYDEESCNPAKDADYYAGLYYLQEPSAMTPASRLPIE
                     PDDRVLDLCAAPGGKATELGSRIGEGGMLLANDISNSRAKALLRNLEIQGVGRLLVTS
                     EDPEKLVTLYPAFFDKILLDAPCSGEGMFRKESSMLRYYSENGPEHYVPIQKKLIEQA
                     YQMLAEGGELLYSTCTFSVKENEEVIAGLLDAHTDMEVQEITPGYEGFAPGVSVNGRD
                     LSRCVHIFPQRMEGEGHFVALLKKQGESRKRQPSRLLETTKKLPKEAEEFLAGVRMDW
                     KNGSFALVKDQLYFLPEGVCAAKGLRYLRTGLSLGTVKKNRFEPSQALAAYLKKEEYV
                     SCLTIPKGDDRVMRYLKGETLSFSEEECQGKKGWVLVCLDDFPLGWGKINNGTLKNKY
                     YAGWRMV"
     gene            complement(13420..>13689)
                     /locus_tag="PLN77_03810"
     CDS             complement(13420..>13689)
                     /locus_tag="PLN77_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRM43441.1"
                     /translation="IVLDNEMSSVRSEIMDAQNSSQMQRNLITDILNNIENLYKVTNK
                     REVEKIQDEFYHIYAQNDLQELKRFHKELDLIAEGYRAQAITDEL"
BASE COUNT         2930 a         3786 c         3163 g         3800 t
ORIGIN      
        1 cccataaaca gaataccaaa tcccaagata acctcgccga gacggctgat cgtcagattg
       61 ttctttacga acataaccat cagaacaccg atgatgacaa acagcggcgc gatatccgca
      121 aggtcaaacg caatcagctg tccggtaact gtcgtaccga tgtttgctcc gaggataatt
      181 ccaggagcct gtgccagttt catcagaccg gagtttacga agctgaccac cataacggtt
      241 gtagcgttgg aggactgaat cagcgcggta aagaaaatac cgaccagaag accgatcaca
      301 cggttcttcg taaatacctc cagaatgctt ctcatcttgg atccggcaac cttctgcagt
      361 ccttcactca tcagcttcat tccatataag aagaagccca gaccgcccat caaagacact
      421 attacactca tattcttttt tcttccttgt ctttttctga tgtcttttca aaatccctgt
      481 caaagtattt tcgtgttttg gtctcaatat tttacattta cttaacgcag attttacaag
      541 acaccttttt tatcataaaa gaaatcatga catttgtcta tagaaaaatt aaaaatttcg
      601 acaagaaatt ttcagattct tttcgtttct ttcttgcatt ctacttgata atattgtaac
      661 atttaggatg tttccatatt atgaggcgac atttccgcct acaaaattca aaaggagaga
      721 aaactatgga aaagtttagc aagctgatgg aaaacacatt caagctgcgg gaaaatggaa
      781 caacggtcaa aaccgagatc atcgccggtg tcaccacgtt catgacgatg gcgtacattc
      841 ttgccgtcaa cccgaacatc ctttcctcca ccggcatgga ccacggcgct gtctttaccg
      901 caacggctct ggcagccttc cttggcaccc tgctcatggc acttttcgcc aattatccat
      961 tcgctctggc accaggaatg ggcctcaacg cctatttcgc ctacaccgtt gttctcggct
     1021 acggatatag ctggcaggca gccctgaccg ccgtttttgc agaaggtatc atctttattg
     1081 tactttctct gaccaacgtc cgtgaggcga tcttcaactc cattccggcc agcctgaaat
     1141 ctgcagtcag cgtcggcatc ggtctgttca tcgcattcat cggacttcag aatgcaaaaa
     1201 ttgtactgcc gaactcttca accgtggcag gcctctattc cctgtcttcc tacaatgcca
     1261 acctgcagtc tgcagccaca ttaaacggaa cagagtatgt ggcaggaacg tttaacgatg
     1321 tcggaatcac cgttctgctc gcagtcatcg gtgttatctt caccgccgtt cttgtcgtac
     1381 gcaatgtaaa aggaaacatc ctctggggaa ttcttgcaac ctggatcctc ggcatgatct
     1441 gccaggcatg cggtctctac gttccgaacc cggcaaacgg cttctacagc ctgttcccgg
     1501 atttttccaa cggactttcc attccaagcc ttgccccgat tttcggaaaa ctggatttct
     1561 ccattctgaa aacaggcgag ttcttcgtga tcatgtttgc attcctgttt gtcgatatgt
     1621 ttgataccat cggaactctg atcggtgtat cttccaaggc aaacatgctc gacaaaaacg
     1681 gaaaacttcc gcgcatcaag ggcgcgctga tggcagatgc tgttgctacc tgcgcaggtg
     1741 ctgttcttgg tacttctacc acaacgactt tcgttgagag tgcttccggc gtttccgaag
     1801 gcggccgtac cggtcttacc gctgttacca ctgcaatcct cttcggcctc gctctgttcc
     1861 tctccccgat cttccttgcc atcccgtcct ttgcaacggc tccggcactg atcgtggtag
     1921 gcttctacat gctgaccaac gtagtcaaca ttgatttcag cgatctttcc gaggcgattc
     1981 cgtgctacat ctgcatcgga gctatgccgt tcttctacag catttccgaa ggtattgcca
     2041 tgggagttat ctcctacgtt gcaatccaga tcctgagcgg aaaagcaaaa gaaaaaaaga
     2101 tcagcgtgat catgtacatc ctggctgttc tgtttatctt aaaatacctt cttctgtaat
     2161 ggctttttaa cgatacagaa ccgcataaaa aggctcctgt cccacagatt ttattctgtg
     2221 aaacaggagc tctttttatg ctctgaatca aaaattttta ttctgccagg gtcctgtaaa
     2281 agcaggtgcg gtttccggtg tggcacgctg cgccgacctg ttctactttg gcaaggattg
     2341 tatcgttgtc gcagtcgatc agaagctgtt ttacatactg cacatggccg gagctcagtc
     2401 ccttcgtcca gagctcattg cggcttctgc tccagtacgt catttttcca aggcgcagtg
     2461 tcgtctcaaa cgcttcttca ttcatgtagg cgagcatcag cacttcgtca ttttccgcat
     2521 cctgtacaac aaccggaacc atgccgtcgc tgtttgtctt aaaatccgcc caggtaaaat
     2581 cacattttaa tgcttgtttc atctcttgtt ctcctctttt tctgctctta caggctgccc
     2641 ttggtcgaga gcacgccggc aattctcgga tcgaaggaag ttgcctcatc gagtgccttc
     2701 gcaaatgctt tgaacatcgc ctcgatgatg tggtggttat ttccgccgtc aagcactttg
     2761 atatgcagat tcatctgcgc cgcataggaa acggcgtaga agaattcttt taccatctcg
     2821 gtctccatct ctcctacttt ctcccgtgta aattctgcct ggaaagagag atacggtctg
     2881 ccgcagaggt caacggccga gagcaccagt gtctcgtcca tcggaaggat tctgtcgccg
     2941 tagcggcgga tgccctgttt gtcgccgact gcctcgcgga ttgcagttcc gagcacgata
     3001 ccggtgtcct cgatcgtgtg gtggccgtct acatcaagat ctccgtctac agaaacggaa
     3061 aggtcgaaaa gaccgtggcg ggcaaagccg tcgagcatat ggtcaaaaaa gccgatgccg
     3121 gtgtggatgt cgctctttcc cgttccatcc agatttaatg tcatgcggat ctttgtttct
     3181 tttgtcacgc gttctacgct ggctgttctg ttctccactt actcttcccc ctcaaaacgg
     3241 acggcaatcg aattggcgtg tgccgtcaag ctctctgatt ttgcaaagct ctcgatatcc
     3301 tcgtggatct cggaaagggc ttctctggaa taatagatga tgctggattt cttcacgaaa
     3361 tcgtctacgg acagcggtga gaagaatttt gcggttccgt tcgtcggcag gatatggttc
     3421 gggccggcaa aatagtcacc gagaggctcg ctggagttct cgccgaggaa aatagcaccg
     3481 gcgttatgta ccttcatcat atcctcaaac gggttcttcg tcacgatctc gaggtgctcc
     3541 ggtgcgatgg catctacggt gtcgatcgct tcctgatgcg aatcggtgac aaggatgtag
     3601 ccgaagttgt ccagtgattt ggagatgatg tcgcttctgg agaggacttt taagaaaccg
     3661 tctacctctt cacttacctt ctctgccagt tcctgggagg tggtaatgag gatcgcactt
     3721 gcaagctcat catgctcagc ctggcttaag agatcggctg ccacgaaccg cggatttgca
     3781 gtctcatcag caaggacaag aatctcactt gggcctgcga tggagtcgat gctgacattt
     3841 ccgtatacgg ctttctttgc aagggcaacg tagatgtttc caggtcctac gattttatcg
     3901 actttcggaa tgctctcggt tccgtaagca aaggctgcaa ttgcctgtgc accgccgatt
     3961 ttaaagacgc ggtccgcacc ggcttctttt gctgccgcaa ggacaaccgg gttgactttt
     4021 ccgtctctgc cgcacggcgt tgtcatgcag atttctttga cccctgccac ttttgccgga
     4081 agaatgttca tcagaacgga ggacggatac gctgcctttc ctcccggaac gtatacgccg
     4141 acacggttca gggcggtcag cttctggcca agcatcgttc cgttctcggt ggaggtgaac
     4201 cagctgttct gtttctgttt ttcgtggaac tcacggatat ttttcatgga tctgcggatg
     4261 acaccgatca gctctggatc tacgatgttg tacgcttcat caatctcttc ctgtgttact
     4321 tctacggtag aagcatcgag taccgcgtgg tcgaatttct cggtatattc gaaaacggct
     4381 gcgtcttttc ttgtttttac attctcaatg atctcctgca cggaagcctc gtacgttccg
     4441 tactgtgtcg ggcttcgttt cagcatattc tccagaatat tttctgtggt ctcttttgtc
     4501 agttttacga ttctcatacc agtgctcctt tcagatcctg aattaagttg gtaattctct
     4561 cattttccat ccgcatgctc acctggttga caaccatacg cgcagacagc gggcagacct
     4621 cctcgagaac ggtcaggccg ttttcccgta aagtgcttcc tgtctctaca atatccacaa
     4681 tcacttcgga aagtccgaca atcggtgcca gctcgatcga gccgttcagc ttgatcatct
     4741 caacggtctg gtgcttgcgg ttgtagaagt aatccttggc aatgtttggg tacttcgttg
     4801 ccacacggat ctgctggttg tgctgcagca gctcacgcgc ggattccggt ccgcagacac
     4861 acatgcggca cttgccgtag ccaaggtcca atacctcgta gaggcgtctt ccttcctcca
     4921 gaatcgtgtc ctttccgacg attccgatat ccgctgcgcc gtactccaca taggtcggca
     4981 catccggacc tttggcgagg aagaaacgga gcttatgctc ttcgtccgtg aaaatcagct
     5041 ttctcgtatc tttatttttc atttcatcgc aggtgatgcc gactttttcc agcagctcca
     5101 gcgtctggta cgccaggcgt ccttttgtca gtgcaatggt caaatatctc atctttttcg
     5161 tacccttcct ctcctacagc tccaccggct gtacttctcc tgtcgacaaa ttgatggcaa
     5221 gcaccgaatc gctcttctga atatagacaa tgccgccgaa ctggttcttt ctgccgtatt
     5281 cttcataatc tgcaagaacc ttgtcccttg caaagcagac cagcgcgaca tccatgcctt
     5341 tgcttctgtg ctcgttggca agttttacgg ccagcggctg aaggaaatcc gggtagagca
     5401 gcatcgtttt cgtattgaca atcggaagag aaattttctg gcgctccagt gcattcatca
     5461 gagattccat gacagtacag aatccgatcg ccggcgcctt ctttccgaag tgctgcagca
     5521 gtgcatcgta gcggccgcct tttacgatcg gctcgccggt gccgtaggtg taggcctgga
     5581 aaataatacc ggtgtagtaa tggaatttgc ttaaaagacc gagatcgaag aagacgtatt
     5641 tttcatagcc gtaatcttta acgatctcat agatcgtagc cagacgatcg agtgcctctc
     5701 ttgccgccgg atttcttgta aggccttttg ccttttcgag aatctctgca tttccgaaca
     5761 gctgcggaac atcacggagt gcttcaaaca gatcacttcg aaggttctgt tttgccagca
     5821 gctcctcggc gccaaaatga tttttaatgg aaatcagttc ttttaattcg ttcagaacat
     5881 cctcggagag agcagcttcc tcggcgagcg cctggaagaa acctacgtgg ccgatgctga
     5941 tctggaaatc ggtaagaccg gcatccagca taacttttac ggtcatcgca atgatctctg
     6001 cgtctgccat tgcggaatca tcgtttaaga gctcgacgcc catctgggtg gactctttta
     6061 agcggccctg atagctgctg ctgttgacga agctgcttcc tctgtagcaa aggcggatcg
     6121 gcatcgtctc ttccatgaaa tacatggagg ctgcacgtgc aatcgacggg gtaaagtccg
     6181 gacggagcac aagggtgttg ccgtcgcggt caaagaattt gtagagctcg cgggacggtg
     6241 ttgttcccac ttctcttccg aatacatcaa aaaactcgaa cgtcggtgtt tcaatatcct
     6301 gatatccaaa cgcatgaaat acctgctcaa tctgattctc caggtataat tttttcgtac
     6361 attcctgatt gtagacatcc cggacgcctt ccggggtatg aattaatcga tccatgtttt
     6421 gttgtcctca ctttttcgct ttagttcgct accatattac catattgaat ttaaaactat
     6481 ggttagtata tcggaaaaca ttttccctgt caacccaaaa ttttaatccg ctttcgaatc
     6541 ccgtatctcc agatccttct ggattccgtt caggtacggg acgagaacgc tgcttcccgt
     6601 tctgggaagg aagaatgacg tttcaagctc ggtgtaggaa aaatgcttct ttccgccgtt
     6661 ctgtccgcgg tcccagaagg ttttcatcgt ctcataccgc aggtaataat attcgtcccg
     6721 cgccgtaaaa tacagcagca ggaaggaaat ccccccctgc tgctcgaaat ctttcataaa
     6781 cgtgacctgg tgctcgtgga tgtttgcgag cggaaaggaa tccgttttgc actctttggc
     6841 atcgaagcag acgggaatgc cctgtacagc gccgatgtaa tcaacggtac tcttcttttc
     6901 aaaataagcc agcgtgatgt gtctgctctc cttatctatt ttgatcgggg tgatcggcgt
     6961 cggcaccttc tggatcagtg ccagatgctt gtcacggtac tgctcgtttg tgcggttgat
     7021 catttcctcc agcgttgatc cgcggagtcc cctcgatgtc catgccataa ttcctgtcct
     7081 cgtcttcgtt ctgttcttat tcaatcagag aaattttgtt cagcggccag tagcgcagaa
     7141 aggccttgcc gatgatcttg tctttctcta cgtacggatt cagccagtag cgggaatcct
     7201 ttgatacgtt ccggttgtcg ccgagcataa agtagcagcc ctccggcacc tcatacggtc
     7261 cgaaatcgcc gaccggtgtt tccgggcaga aatcatcgcg cagcggctcg gtggaatcgt
     7321 tgatatacac ctttccgtcg cggatcgtaa ccgtctctcc cggcagtccg atgacgcgct
     7381 tgataaactt ctgcttctcg tcgtccggat aatagaaaat aacgatgtca taccgctgcg
     7441 gatcgctctt tacataagca aggcggttac cgaagatctg gtctccggtc ataatcgtgt
     7501 tttccatgga aggagatggg attcnnnnnn nnnngatgac aatgacctgc tccacaaaaa
     7561 caacgactgc cacgacaatg gcgatcattt ttacatattc ccagatttcc cgtccgatgc
     7621 tcgtctcctc cgtttttctt ggttttgttt ctttcttttc gttgtctttt cggctcataa
     7681 tccttctccc gtcaaatgtt ctccctgtaa aatccggcgc acggatgatg gcaggggcgc
     7741 tgtaaaggtt tttccggaca gatccgataa ggtgcccgta agctctggaa ataccgcctt
     7801 ccacgaatgg agaagttggt atttcagtcc gtatttttcc cggaagcggc ggttaaactc
     7861 cgcatccccg tatttcgtat ctcccgcaag cggatgaccg atgctcgcga gatggctgcg
     7921 gatctgatgg ctctttccgg tcaccagctc aacctccagc agcgtacagc ctcttccggc
     7981 acagagcggc cggtatgcgg tttctatgta aacagcatct ccctgctgct ctctctgaat
     8041 cgaaacacgg tttgtcctct cattttttat gaggtatccg cggatccgct ctgtctctgt
     8101 cacggttcct tttacaaccg tcagatagta ttttccaaca gcccgctctg cgatcagttc
     8161 tgaaagctgc tgcgtcgccg caacggtttt tccggcaaga atcagaccgc tggtgttccg
     8221 gtccagacga ttgcagagtc cggcgcggaa catggtctcc tccccggcat ttttttccga
     8281 aagataggcg ccgagcgctt cggtaaggct cacatccgtc tctttcgcct tctgtgagag
     8341 gacacctgcc ggcttgttca ggaaaattac tttttcgtct tcatagacga tatccagctt
     8401 cggatacttt acctgttttc cagccgccgg tttttccggt gttttccgaa atttttcgat
     8461 ggtctcttcc gccagataca gccggatcgt atcgcctgtt ttcagctttt cactgccgtc
     8521 cgcctttttc tggttcagca caatgttttt ctttcgcagc attttataga agaaactctt
     8581 tggcgcctcc tccatatatt tggacagata tttgatgagc tgctgcgcgc agtctttttc
     8641 tgtcacggtc agttctctca tagcaatggt ctcctacagt gccagcgcca gcatcgtgtg
     8701 tttcaccagt tccgtgcggg acagatccgg gtaccgctca tccggtttga agttttcact
     8761 ggcgctcttt tccagctcat cgtagttgcg gttcaaggaa tcgaggcgct ccagaaacgc
     8821 tttttcccgc tcaaaaatct tcacatggca tttgtagccg ttggcacgga tgatcttgtt
     8881 cagatacgta gtgcagtcct cgatctgttt ctggtctttg gcgcgcgagg tgtagcaggc
     8941 gatctcctca ccgcagaacg ccgctgcttc gatcatcaga ctcgattttt cctgtgtgat
     9001 atacatttca tatcccatca gcagttttcc tttttttgcc tcaatggcat cgtacactgc
     9061 tttgtccact ggcttttgca taaccatcat gataaaaccg acggcggcgc ggcagcccgg
     9121 cagacagcct actacggcga caaccgtaaa aatgttgctc ttgtttccgt ctgcaagcac
     9181 aagcccgaca ataaaaacga tggccggaag gacgaacagt cccagtgtca caagcagtct
     9241 ttttttcttt tcccggcgga tatagccgta atcccctttt ctgatcttca ttcttctgtc
     9301 atttcccttc ttcgataaga tccccgctca tcttctgcgt gtttatttct ttttgtgcac
     9361 gttgcggagc gtctttttcg gccgtccgcc tgttttggct tttccctgtc cggaacggtc
     9421 attcgccgtc ttctggccaa agccttttcc gttggaccct tttcctcttg acggttttcc
     9481 tgcctccccg gccatctttc tcggccgtac gagacatttc ggtccgaatc cgatcagatc
     9541 ctcccggtgt gtccggcgca gtgcctcgca gacgagctca tagttctccg gattccggta
     9601 ctgaatcagc gcacgctgca tcgccttctc atgcggcgat ttcggcacgt aaaccggatc
     9661 catcgtccgc gggtcaagac cggtatagta catacaggtg gaaagtgtgg aaggcgtcgg
     9721 atagaagtcc tgtgcctgct ccgggataaa tcccatatcg cggatgtact cggcaagctc
     9781 caccgcatcc tccaagtcac accccggatg ggacgacata aaatacggaa gtgcgtactg
     9841 ctgcttcccc gtctttttgt tgcacgcatc gaagcggcgg ataaattcct gatatacttc
     9901 gtggcgcggt tttcccatat aggaaagcac acggttcgaa acgtgctccg gcgcaacgcg
     9961 gagctgaccg ctgacgtgat cctttaccag ctcggaaagg aacgtctggt ctttatcggc
    10021 caggacatag tcaaaacgga tgccggagcg gataaagact ttcttcccgc agcagcttca
    10081 cataatctct gtgatctgcc accatatttt tacacggctc cgggaagaga cagttccggt
    10141 tcgggcaggc gcctttggtg agctgctttt tgcatgccgg accgcggaaa tccgccgtcg
    10201 ggccgccgac atcgtgaata tagcctttaa attctttttc ctgaaccatc tgccgcgctt
    10261 ccgccagaat ggattcgtgg cttcttgtct gcacaacgcg tccctcgtgg aacgtcagcg
    10321 cgcagaaact gcaggatcca aagcagccgc ggttgctcgt cagactgtat ttgatttccc
    10381 caagcgccgg cacaccgccg tctttttcat agcttgggtg ataggcgcgc atgtatggaa
    10441 gcgcatagac atcatccatc tcctgctctg tcaaaggacg ggaaggcgga ttctgtacaa
    10501 tgtagccctg gccctcatac ggctctgcca tcggcttcgc attgatggca tcggtatttt
    10561 tatactgaat cgcaaagctc tccgcatacg tccgcttgct ctcccgcacc tcctcgaatg
    10621 acggcagcac caccgggtca atcaggtgat ccagcgtttt gctccgataa acggttcccg
    10681 gaataaaggt gatatcatga acgtcaatgc cagccgcaag tgcatctgca atcgctacga
    10741 tcgaaagttc tcccattccg tacgaaatca gatccgcacc ggaatcgaga aggacggagc
    10801 ggcgcacctg gtcagaccag taatcgtaat gcgaaaggcg ccggagcgat gcctcgatgc
    10861 cgccgaggat catcggtgta tgtttatacg ttctccggat cagattgccg tacacggtaa
    10921 ccgcgcggtc cggccgtttt cccatgacac cgcccggcgt aaaggcatct gtttttcggt
    10981 gctttttcgc cacggtataa tggttgacca tggaatccat atttccggaa gagacaagaa
    11041 agccaagacg cggttcaccg aaaatcgaaa tgctgttctc gtccttccag tccggctgtg
    11101 agatgatgcc gacggaatag ccgttcgcct cgagcacacg gctgatgatt gccggtccga
    11161 acgaggaatg gtccacgtag gcatctccga tgacatagac aaagtcaaac tgttcgattc
    11221 cccgttcctt catctcttct cttgtaattg gtaaaaaagg catgctttac tttcctttct
    11281 gtgtttcggc gtccgctatg ccaagcgccg ctttttcttc ctgcgtcagg agacgggcac
    11341 tgccctttgg cagcgccgga tcaagtttca ggctccccat cgacagccgt ttcaaataga
    11401 cgaccgtttt tccgaccttc tcaaacatgc gcttgatctg gtggaagcgg ccctcacaga
    11461 tggtaatgcg gatccggctt tcaaaaatgc caggctcttt ttcagtcgtt tccagaatgg
    11521 taagctcggc cggaagtgtc ggtttgtcgt ccccaatgtc gagcccttcg gcaaatgcct
    11581 gccgatcttc ttctgtcact tttccgtcaa tcacggcctc atacactttc gagacatgcg
    11641 atttcggaga gagcagccgg tgggaaaggg ctccgtcgtt cgtaatcagc agcagccctt
    11701 cggtgtcctt gtcgagccgt ccgaccggaa agaggtcttt tcttgccgca tcacttaaaa
    11761 gatcgagtac ggtccggtca cggcggtcct ccgtcgcaga gacgacaccc gccggtttgt
    11821 ggagcatcca gtattccatt ttcacatagg aaatgctgtt tccgtccaca gtcacctgcg
    11881 cttcatccgg gtcgactttc agttccgggg cttttgccgt aattccgtct acggccaccc
    11941 gtcccttccg gatcagggcc tttacttcct ggcgtgttcc cacacccatg tccgccagat
    12001 atttatcgag tcgtaattta ctcataccat ccgccatcct gcataatatt tattctttaa
    12061 cgttccgttg tttatttttc cccagccaag tgggaaatca tccaggcaca caagcaccca
    12121 gccctttttc ccctgacact cttcctcgga aaacgagagt gtctcaccct ttaagtagcg
    12181 catgacacgg tcatctccct tgggaatggt caaacacgaa acgtattctt cttttttcag
    12241 atacgccgcc agcgcctggg acggctcgaa gcggtttttc tttacggtgc caagcgaaag
    12301 tccggtccgc agatagcgca gaccctttgc cgcacagaca ccttccggca gaaaatagag
    12361 ctggtctttc acaagggcaa acgaaccatt cttccagtcc atgcgcacgc cagcaagaaa
    12421 ctcttccgcc tctttcggca gcttctttgt cgtttccaaa agtcgggacg gctgccgttt
    12481 ccggctctct ccctgctttt tcaggagcgc cacgaaatgg ccttctccct ccatccgctg
    12541 cgggaaaata tgaacgcacc gggaaagatc gcggccattc acggatactc ccggcgcaaa
    12601 cccttcgtaa cccggggtaa tttcctgcac ttccatatcc gtatgcgcat ccagcagccc
    12661 tgcgatgacc tcttcgttct cctttacaga aaacgtacag gtagagtaca gcagctctcc
    12721 gccctctgca agcatctgat acgcctgctc gatcagtttt ttctggatcg gcacataatg
    12781 ctccggccca ttttcactgt agtagcgcag cattgagctc tcttttcgga acatgccctc
    12841 cccagagcac ggcgcgtcaa gaaggatctt gtcaaaaaag gccggataca aggttactaa
    12901 tttttccgga tcctcgctcg tcacgagaag acgtcccact ccctggattt ccaggttgcg
    12961 cagcagcgcc ttcgcccgcg aattgctgat atcgtttgcc agcagcatgc cgccctcacc
    13021 gatccggctt cccagctccg ttgcctttcc gcccggtgcc gcacagagat cgagcacacg
    13081 gtcatccggc tcaattggaa ggcgggatgc cggtgtcatc gcactcggct cctgcagata
    13141 atacaggccc gcatagtaat cggcatcctt tgccggattg cagctctctt catcgtaata
    13201 aaatccattg gggatccacg gaaccttctt aagcggaata tcggtccgcc taagaaactc
    13261 ttctgtcgaa atttttccgt tgtttacccg cagtcctcta tgtaccgcct cgtcaaatgt
    13321 cttgcggtat gcctcatatt cttcctcacc gagcagctgt ttcatctcct gctcaaaggc
    13381 ttccggcagt gtgatcatat ccgtttatcc ccttttattc taaagttcgt ctgtaattgc
    13441 ctgtgcgcgg tatccctctg caatcagatc cagttctttg tggaaccgtt ttaactcctg
    13501 cagatcgttc tgcgcataga tgtgataaaa ctcatcctga attttttcta cttcgcgttt
    13561 gttcgtcacc ttgtaaagat tctcgatatt attcaggata tccgtgatca gattccgctg
    13621 catctgggag gaattctgtg catccataat ctcactccgc acagaggaca tctcattatc
    13681 cagcacaat
//
LOCUS       DAOMUF010000039        13607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1012_length_13607_cov_3.984578, whole genome shotgun sequence.
ACCESSION   DAOMUF010000039 DAOMUF010000000
VERSION     DAOMUF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1012_length_13607_cov_3.984578"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /gene="lysS"
                     /locus_tag="PLN77_03815"
     CDS             complement(<1..1679)
                     /gene="lysS"
                     /locus_tag="PLN77_03815"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRM43442.1"
                     /translation="MAQQTQEQDINHLLKVRREKLAELQQNGRDPFQITKFDQTHHSL
                     EVKGLYEAHETELLKDRQEPNVEGMDEEQAKEALKKDYEERRNIMDASPIHVAIAGRM
                     MFKRVMGKASFCNIQDLQGSIQVYVARDAIGTESYADFKKSDIGDIFGVEGFAFRTRT
                     GEISIHAEKVTLLSKSLQILPEKFHGLTDVDTRYRQRYVDLIMNTESKDTFIKRSKIL
                     SAIRKYLSGEGFMEVETPMLVQNAGGAAARPFETHFNALNEDLKLRISLELYLKRLIV
                     GGLEKVYEIGRVFRNEGLDTRHNPEFTLMELYQAFTDYHGMMDLTENLYRFVAQEVLG
                     TTQIVYKGIPMDLGKPFERITMVDAVKKYAGVDWNEVETLEQARELAKEHHIEFEERH
                     KKGDILNLFFEEFVEEHLLQPTFVMDHPVEISPLTKKKPENPEYVERFEFFMNGWEMA
                     NAYSELNDPIDQRERFKAQEELLAQGDDEANTTDEDFMNALEIGMPPTGGIGFGIDRM
                     CMLLTGAEAIRDVLLFPTMKSMGAAKNEANNAAQSAPAEKTVEKAIAEVKET"
     gene            complement(1708..2190)
                     /gene="greA"
                     /locus_tag="PLN77_03820"
     CDS             complement(1708..2190)
                     /gene="greA"
                     /locus_tag="PLN77_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRM43443.1"
                     /translation="MEQKKTILTYAGLKKLEDELHDLKVNKRREIAGKIKEAREQGDL
                     SENAEYDAAKDEQRDIEARIEEIEKILKNAEVVVEDEVDSDKINIGCKVDVLDLEYDE
                     EIEFQIVGSTEANSLEGKISNESPVGKALIGAHKDDIVDVETPAGIMQYKVLSIQRNI
                     "
     gene            complement(2423..3817)
                     /locus_tag="PLN77_03825"
     CDS             complement(2423..3817)
                     /locus_tag="PLN77_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM43444.1"
                     /translation="MTIKELFAPSDMTVGKPWEKIVAFTIPMLIGNIAQQLYNTVDSI
                     VIGKYVGDNALAAVGSASPILNLLLVLFVGISVGAGVMVSQYFGARQRKELSMMIGNC
                     VTLTAISSIIIMILGAVLSRPLLEMLKTPDSIIDWCTSYLVILMVGCAGSAYYNILCG
                     ILRGLGDSISALIYLLVATVINIVLDIVFVSQFHMGVAGVAYATVIAQAISAVLCMWR
                     LMRMTDIFDFRPKYLKMKKQYSMEIIRLGLPSGITQAIFSMAMVIVQSLTNSFGEMVI
                     AANVIIMRVDGFAMMPNFSFGTAMTTYAGQNVGARRMDRVEQGAKQGTLIAVGTSTTI
                     TLLIVIFGHHLMGIFTNTTELVTLSANMMKILAVGYIAMAITQSLSGVMRGAGDTMTP
                     MWISMVVTVVIRVPLAYGLAFLTRTPELPNGRYQVLWISMLVSWCMGALLTLIFYKKG
                     NWKKKALTGSSSEK"
     gene            complement(3947..4945)
                     /gene="dusB"
                     /locus_tag="PLN77_03830"
     CDS             complement(3947..4945)
                     /gene="dusB"
                     /locus_tag="PLN77_03830"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRM43445.1"
                     /translation="MESKKRTLQTMSPLKIGNVEMQNPLVLAPMAGVTDLPFRVLCKE
                     QGAGLICMEMVSAKAILYKNKNTEDLMTIHPGEHPVSLQLFGSDPEILAQIAAQIEER
                     PFDILDFNMGCPVPKVVNNGEGSALMKDPALVRKIVTGMVKAVKKPVTVKIRKGFNEE
                     SANAVEIAKILEDCGVAAIAVHGRTRAQFYSGKADWDIIRQVKEAVSIPVIGNGDVVD
                     AASAEALVEQTGCDGIMIGRGAEGNPWIFKQILHYRETGETLAKPTQHEVKEMMLRHA
                     RMQVEYKGGAIGIREMRKHVAWYTAGFPHSAKLRAAINQVESLQELEQMLNEWERG"
     gene            complement(4935..5318)
                     /locus_tag="PLN77_03835"
     CDS             complement(4935..5318)
                     /locus_tag="PLN77_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6145 family protein"
                     /protein_id="HRM43446.1"
                     /translation="MYSDDEKVVLCGASAYEQKYYFNQDFASLPQSVQDELHIMCVMF
                     TVEIGGIFTMWFDSDGSLQFETEAVDADAMYDEIGGALRIKQYQEEKKDLLESLELYY
                     RVFFLGEEVPEEAFAEEDGEKPDGK"
     gene            complement(5346..6338)
                     /locus_tag="PLN77_03840"
     CDS             complement(5346..6338)
                     /locus_tag="PLN77_03840"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRM43447.1"
                     /translation="MLENKRAETLRLLRESGENVSGQDLCEHFGISRTAVWKIMNQLK
                     EEGYEIEAVQNKGYRLLNEPDLMTKDELESRTHTKYVGQTVYYSHEVDSTNTWAKRLA
                     EEGAPNGTLTTAETQTAGKGRRGRVWKSPEGTSVSMSLLLYPDLEPAKAPMLTIVMGL
                     AVVQGVQRALGVDTRIKWPNDAVLNGKKLCGILTEMSAQIDYINYVVIGTGINVNQTE
                     FPEEIAEIATSLAIEMGHPVNRAKIVAAVLEAFEENYEKFLEAGDLSALQEAYEAVLA
                     NKDQPVRVLDPKEPFEGVALGITSTGELRVRKEDGTIAEVHSGEVSVRGLYSYV"
     gene            complement(6467..7456)
                     /gene="argF"
                     /locus_tag="PLN77_03845"
     CDS             complement(6467..7456)
                     /gene="argF"
                     /locus_tag="PLN77_03845"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRM43448.1"
                     /translation="MDLKGRDFLTLKDFTPEEISYLLDLSAELKDKKKKGIPVDTLRG
                     KNVALIFEKTSTRTRCSFEVAAHDLGMGTTYLDPTGSQIGKKESIKDTARVLAGMYEG
                     IEYRGFGQEIVEELAKYSKVPVWNGLTNEYHPTQMLADMLTIQEEFGHLKGIKLVYMG
                     DARYNMGNSLMIASAKMGMDFVACAPKKYFPNAELVAQCEEYAKESGATITLTEDVKE
                     GTKDADVIYTDVWVSMGEPDSVWEERIHDLTPYRVTKEAMANAKDTAVFLHCLPSFHD
                     LKTKIGKEIHDRYGLDEMEVTDEVFESAQSRVFEEAENRMHTIKAVMAATLGA"
     gene            complement(7735..8703)
                     /locus_tag="PLN77_03850"
     CDS             complement(7735..8703)
                     /locus_tag="PLN77_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRM43449.1"
                     /translation="MSGGGIAGILMIFLLVIVLFIAASCVKIVPQASAYVVESLGKYK
                     GTWATGLHFKVPFIERVAIRVNLKEQVVDFPPQPVITKDNVTMQIDTVVFFQITDPKL
                     FAYGVVNPLMAIENLTATTLRNIIGDLELDETLTSREIINTKMQAALDIATDPWGIKV
                     NRVELKNIMPPAAIQEAMEKQMKAERERREAILIAEGEKKSTILVAEGKKQSAILDAE
                     AEKQAAILRAEAEKEKRIREAEGQAEAILKVQQANADGIRAIKEAGADDAVLTLKSLE
                     ALGVLANGKATKIVVPSDLQGLASLATTFKGIVEEQKGPAGKEGQK"
     gene            complement(8700..9155)
                     /locus_tag="PLN77_03855"
     CDS             complement(8700..9155)
                     /locus_tag="PLN77_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRM43450.1"
                     /translation="MEYAVYWLIALVVLLVIEAATLGLATIWFAGGALIALIAAMCGA
                     GFVIQMVCFLVVSLILLIFTRPVAVRFLNKDTLKTNVDRVIGMEGIVAEEISNLAGTG
                     KVSLGGNTWTARTESEGGIIPKETVVEVLRVEGVKLIVKVKEKEGGNET"
     gene            complement(9551..10594)
                     /locus_tag="PLN77_03860"
     CDS             complement(9551..10594)
                     /locus_tag="PLN77_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM43451.1"
                     /translation="MFYPLVSVIIPVYQAEKFLTECVESVRKQDYSELEIVLVNDGST
                     DRSNQMCEELAAADPRIRVIHQNNKGSGAARNAGIKEATGKYLLFLDADDKLDDSSAI
                     RSLAARAEREQADIVIGTYRRWRANGEKVKCCSNLEKENPDSAEFRFHGFFQKGTLSY
                     DWGKLYRKSFLESHDLWIPPYSYAEDKAHNFRCCACHPKYAFVPQSIVLYRENLQSLT
                     YQPKKNLMRNWILIASDFEQFLKEKHLSREYGDLIFFHLLIGAMYLAKEEMTYQGKKI
                     RVAAKILKQYSRNPFVEQKLTLKECIRYTRQIKSLFWKLLAFTLVLFIQMHMYFVVAA
                     VFVWMSSLGIDSL"
     gene            11477..12493
                     /locus_tag="PLN77_03865"
     CDS             11477..12493
                     /locus_tag="PLN77_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stealth CR1 domain-containing protein"
                     /protein_id="HRM43452.1"
                     /translation="MKKQIIDFVITWVDGSDPLWFRQKQNAKKKQGVSVDIDDRIERY
                     RDWEILKYLFRSIENFAPWVNNVYFVTCGHLPAWLNTECPKLQIVSHKDFIPEKYLPT
                     FNSHSIEWNFHRIKNLSEHFVYFNDDMFLLHPIKPTDFFENGKPVDMLALQPDVANVE
                     DTIMPYIYLNNTMLLAKYFDKRENMKKQPGAYFHIGYPPMYFGYNLLEMAFPRFTGFY
                     TVHGPSPLKKETYRFFWEKEEELLDGVCSHPFRHKDDVSQYVLREYQKLSGDFVPRNT
                     HKFCKYFNLKPENPELVKTITKQTAKCICINDSNFEIPYEHVKRELNDAFSKLFPQKS
                     QFEK"
     gene            12682..13068
                     /locus_tag="PLN77_03870"
     CDS             12682..13068
                     /locus_tag="PLN77_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43453.1"
                     /translation="MTKNYTKPIVTIDSGMAEGVYAASGATKGTLNVTYYGVWDRWGT
                     NGGKGLAQASWSDLDGTITLNINFNDTVDSIETDDASVQKSWSGKTATLTFASTATNP
                     LTIGIHLNHGTSIDDLQLTGFDYSVN"
     gene            13169..13543
                     /locus_tag="PLN77_03875"
     CDS             13169..13543
                     /locus_tag="PLN77_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43454.1"
                     /translation="MSKYVKPVISLDEGMAEGVYAASGCYTASAYIHQTPENGRGDYR
                     IQVNGKHNADHTKEAQVLTISFNQNVTYVSGGAGLISGNETTTLKVKLAYHQNPTDNI
                     GFGDLVVTSDAGLAITGVSITD"
BASE COUNT         3431 a         3459 c         2722 g         3995 t
ORIGIN      
        1 gtttctttca cttcagcaat cgctttttcc actgtttttt cagccggagc agactgtgca
       61 gcattgttag cttcattctt tgcagccccc atgctcttca ttgtcgggaa gagcagcacg
      121 tctctgattg cctcagctcc tgtcagaagc atacacatac ggtcaatgcc gaatccaata
      181 ccaccggtcg gcggcatacc gatctccagt gcgttcatga agtcttcatc tgttgtattg
      241 gcttcatcat cgccctgtgc aagcagttct tcctgggctt taaaacgttc tctctggtcg
      301 atcggatcgt tcagctcaga ataagcattt gccatctccc aaccattcat gaagaactcg
      361 aagcgctcta catattccgg attttctggc tttttcttcg tcagcggcga gatctcaacc
      421 gggtggtcca taacgaacgt cggctgaagc agatgctcct ctacaaactc ctcaaagaag
      481 agattcagga tatcaccttt tttatgtctc tcttcaaact caatatgatg ctcttttgca
      541 agttctcttg cctgctccag agtctcaact tcattccagt caacgcctgc gtacttcttc
      601 actgcgtcaa ccattgtgat acgctcgaac ggcttaccga gatccatcgg gattcctttg
      661 tatacgatct gtgtcgttcc gagcacttcc tgtgcgacaa aacgatacag gttctctgtc
      721 agatccatca tgccgtggta atctgtaaat gcctggtaaa gctccatcag agtgaactcc
      781 gggttatgtc ttgtatcaag accctcatta cggaatacac gtccgatctc atataccttc
      841 tcgagtcctc cgacgatcag tctcttcagg tacagctcaa gggaaatacg aagcttcaga
      901 tcttcgttca gggcattgaa atgtgtctcg aacggtcttg ctgctgctcc acctgcgttc
      961 tgtacaagca ttggagtctc aacttccatg aatccttctc cgcttaaata cttgcggatc
     1021 gcactgagaa tcttggaacg cttgataaag gtatctttag actctgtatt catgatcagg
     1081 tctacatatc tctgacggta acgggtatct acatctgtca gaccgtggaa cttctccggg
     1141 aggatctgta agctcttgga gaggagtgtc accttctctg catggataga aatctcacct
     1201 gttcttgttc tgaaagcaaa tccttccact ccgaagatat caccgatatc cgatttttta
     1261 aaatcagcgt aagactctgt tccgatcgca tctcttgcaa cgtatacctg gatgcttccc
     1321 tgtaaatcct ggatattgca gaaagaggcc ttgcccatga ctctcttaaa catcatacgt
     1381 cctgcaattg ccacatggat cgggcttgcg tccatgatat ttcttctctc ttcgtaatcc
     1441 tttttaagag cttctttcgc ctgctcctca tccattccct ctacgttcgg ctcctggcgg
     1501 tcttttaaca gctcagtctc atgtgcctcg taaagacctt tcacctcaag cgaatgatgt
     1561 gtctgatcga actttgtaat ctggaacggg tctcttccgt tctgctgcag ctctgcaagt
     1621 ttctcgcggc gaaccttcag taagtggtta atgtcctgtt cctgtgtctg ctgtgccaat
     1681 ctcttcactc ccttttcttt gtatttttta gatatttctc tggatagaaa gtactttgta
     1741 ctgcataatt ccggccggtg tttctacatc gacaatatca tccttgtgcg cgccgatcag
     1801 agcttttcct accggagact cgttggaaat ttttccttcc aggctgtttg cctcggtgga
     1861 accaacgatc tggaactcga tttcctcgtc atactccaga tccagtacgt ctactttaca
     1921 tccgatattg atcttatcgg aatctacttc gtcctcaaca acaacctctg cgtttttcag
     1981 gattttttcg atctcttcaa ttcttgcctc gatatcacgc tgctcatctt ttgctgcatc
     2041 gtactcggcg ttctcggaaa ggtctccctg ctctcttgct tctttgattt taccggcaat
     2101 ctctctacgt ttgtttactt tgaggtcatg aagctcgtcc tccagttttt tcaggccggc
     2161 ataggtaaga atcgtttttt tctgttccat tttatacacc ctttctatcg tttctgtatc
     2221 cagtcatatc tgattttatt ctgtaaactc tccggaaatg cgctgtattt tcagtattcc
     2281 cggttgaaat aaggatagac tccgcctatc ctaacaatta aagtagtata aaggattgcc
     2341 cacggaatgt caagaaaaaa agaaacgcag gaaagatttt ctttttcaca aatccttcct
     2401 gcatctttct cttatttttc ctttattttt cggatgagct tcctgtcagt gctttctttt
     2461 tccaatttcc ttttttatag aaaatcagcg tcagaagcgc acccatgcac cagcttacca
     2521 gcatggaaat ccagagtacc tgatatcgtc cgtttggcag ctccggcgtt ctggtcagaa
     2581 aggccagacc gtatgccagc ggcacacgga ttacaacagt aacaaccatc gaaatccaca
     2641 tcggggtcat cgtatcgcct gctccacgca tgacgccgga aagcgactgt gttattgcca
     2701 tggcaatata gccgaccgca aggatcttca tcatattggc acttaatgtt acgagctctg
     2761 tggtattcgt aaagattccc atcagatgat ggccgaaaat aacaattaaa agggtaatgg
     2821 tcgttgaggt tccgactgca atcagtgttc cctgttttgc gccctgctct acgcggtcca
     2881 tacggcgtgc accgacattc tgaccggcat acgttgtcat cgcggtacca aacgaaaagt
     2941 tcggcatcat tgcaaagcca tcgacacgca tgatgataac attcgcggcg ataaccattt
     3001 caccgaagct gttggtcagc gactgtacga taaccatcgc catggagaag atcgcctggg
     3061 taataccgga cggcagacca aggcggataa tttccatcga atactgtttt ttcattttca
     3121 gatatttggg tctgaaatca aaaatgtcgg tcatacgcat cagacgccac atgcagagta
     3181 ctgcagaaat tgcctgcgca atcacggtcg cgtatgcaac tccggcaacg cccatgtgga
     3241 actgggaaac gaatacaata tccaatacaa tgttgatgac cgttgccacc agcagataaa
     3301 tcagcgccga aatcgaatcg ccgagtccgc gaaggatacc acatagaatg ttatagtacg
     3361 cactgccggc acagcccacc atcaggatca ccagatacga ggtacaccag tcgatgatag
     3421 aatccggtgt tttcagcatt tccagcagcg gtcttgagag gacggctccc agaatcataa
     3481 taatgatgga agaaattgct gtcagcgtga cgcagtttcc gatcatcatc gaaagctctt
     3541 ttctttgtct tgctccaaaa tactgcgaca ccatgacgcc agcgccgaca gaaattccga
     3601 cgaacagaac cagaagcagg tttaagatcg ggctggcgct tccaactgcc gcaagcgcgt
     3661 tgtccccgac atattttccg attacaatac tgtcaaccgt gttgtagagc tgctgggcaa
     3721 tgttgccgat cagcataggg attgtaaaag ctacaatctt ttcccacggc tttcccaccg
     3781 tcatgtcgga cggtgcgaaa agttctttga tagtcataca ctacactccc tcttttgttt
     3841 ttttattccc aatacctcta aatgtcagta tagcatagaa aaagtacccg tgatatatca
     3901 atattggcat atcataggta cttttttgac ttattttact tacttgtcag ccgcgttccc
     3961 actcattcag catctgttcc agctcctgca ggctctccac ctggttgatc gctgcgcgca
     4021 gtttcgccga gtgcgggaat cccgccgtgt accacgcaac gtgcttccgc atctcgcgga
     4081 tgccgatcgc gccgcctttg tattccacct gcatgcgcgc atggcgaagc atcatctctt
     4141 ttacctcatg ctgcgtcggc tttgcaagtg tttctccggt ttccctgtaa tgaagaatct
     4201 gtttaaaaat ccacggattt ccttccgcgc cgcggccaat cataatgcca tcgcagcccg
     4261 tctgttcaac cagcgcttcg gcgctcgccg catcaaccac atcgccattt ccgatgaccg
     4321 gaatcgaaac cgcttctttt acctgacgga tgatatccca gtctgctttt ccggaataga
     4381 actgtgcccg cgttcttcca tgcaccgcaa tcgcagccac accgcagtcc tcgagaatct
     4441 ttgcaatctc cacagcattc gccgactctt cgttaaatcc ctttcgaatt ttaactgtca
     4501 ccggcttttt cactgccttt accataccgg tcacaatctt ccgcaccagc gcaggatcct
     4561 tcatcagcgc ggagccctcg ccgttgttga cgaccttcgg caccgggcag cccatgttaa
     4621 aatccagaat gtcaaacggc cgctcctcga tctgtgcggc aatctgcgcc agaatttccg
     4681 gatcactgcc aaaaagctgg agcgatacag gatgttcacc cggatgaatc gtcatcagat
     4741 cctcggtatt tttgttctta tacagaatcg ccttcgcgct gaccatctcc atacagatca
     4801 ggcctgctcc ctgctccttg cagagaacgc ggaacggcag atccgtcaca cctgccatcg
     4861 gtgcgagcac gagcggattt tgcatttcca catttccgat tttcagcgga gacatcgtct
     4921 ggagcgtacg tttcttactt tccatccggc ttctctccgt cttcttctgc gaacgcttcc
     4981 tccggcactt cctcgccgag gaagaaaacg cggtaataca gctccagaga ttccagcaga
     5041 tcctttttct cctcctgata ctgcttgatc cgcagcgcac cgccgatctc atcgtacatc
     5101 gcatccgcat ctacagcctc tgtttcgaac tgcagactgc cgtcactgtc aaaccacatg
     5161 gtgaaaatgc cgccgatttc cacggtgaac ataacgcaca tgatatgaag ctcatcctgc
     5221 acggactgcg gcagggatgc aaaatcctgg ttaaaataat atttctgctc atacgcactc
     5281 gctccgcaga gcactacttt ttcatcatct gaatacatat attttccttt cctgatccaa
     5341 gttttttata cataactgta caggccgcgc actgaaacct cgccggaatg cacttccgcg
     5401 atcgttccat cctcttttct cacacgcagc tcacccgtcg acgtgatgcc gagcgccaca
     5461 ccctcgaacg gctctttcgg atccagcaca cgcaccggct gatccttatt tgcaagtaca
     5521 gcctcatagg cttcctgcag cgccgagaga tctcccgcct ccaggaattt ttcgtaattc
     5581 tcctcgaacg cctccagcac cgctgcgact attttcgccc gattgaccgg atgccccatt
     5641 tcgatcgcca gtgacgtcgc gatctcagca atttcttccg gaaactccgt ctggttcaca
     5701 ttgattcccg ttccaatgac gacataattg atgtaatcga tctgtgcact catctcggtt
     5761 aagattccgc agagcttttt cccgttcagc accgcatcgt tcggccactt aattctcgtg
     5821 tcgacgccga gcgctctctg taccccctgc acaacagcaa gtcccatcac aatcgtcagc
     5881 atcggcgctt tggccggctc gagatccggg tacagcagga gggacatcga aacggaagtt
     5941 ccctccggcg atttccagac tcttccgcgg cggccttttc cagcggtctg cgtctcggcc
     6001 gttgtcagtg taccgttcgg cgctccttct tccgccaggc gcttcgccca cgtatttgtc
     6061 gaatctactt catggctgta atagaccgtc tgtccaacat atttcgtatg cgtgcggctt
     6121 tccagctcgt ccttcgtcat cagatccggc tcgttcagca gacggtaacc tttattctgt
     6181 accgcctcga tctcgtatcc ttcctctttt aactgattca tgatcttcca cactgcagta
     6241 cgggaaattc caaaatgttc acacaaatcc tgacccgaaa cattttctcc tgattctcgc
     6301 agaaggcgca gtgtctcagc tcttttattt tccagcatat gatatcttca cactcctcac
     6361 ataaaccaac agcagcgctg cgaaagacgt gctccgcagc gctgctgtac gtgtcttgcc
     6421 attcttctat tatacacagt ctgttccggt ttttccacca gaaagtttat gcaccaagcg
     6481 ttgctgccat cacagctttg atcgtgtgca tgcggttctc tgcttcctcg aagactctcg
     6541 actgtgcgga ctcaaatacc tcatcggtaa cttccatctc atccagtccg taacggtcgt
     6601 ggatctcttt tccaatcttt gttttcagat cgtggaacga cggcaggcag tgcaggaata
     6661 ccgcggtatc cttggcattt gccattgctt cttttgtcac gcggtacgga gtcagatcgt
     6721 ggattctctc ctcccatacg ctgtccggct cacccatgga tacccagaca tccgtataga
     6781 taacatcggc gtctttcgtt ccctctttta catcctcggt gagggtgatg gttgcgccgc
     6841 tctcctttgc atactcttca cactgtgcaa cgagttccgc gttcgggaag tatttcttcg
     6901 gtgcgcaggc tacaaaatcc atacccattt ttgcagatgc aatcatcagg gaatttccca
     6961 tgttgtagcg ggcgtcgccc atgtaaacga gtttgatgcc ttttaagtgg ccgaactctt
     7021 cctggattgt cagcatatcg gcgagcatct gggtcgggtg gtactcgttg gtcagtccgt
     7081 tccataccgg gactttggaa tattttgcca gctcctctac gatctcctgg ccgaatccac
     7141 ggtactcaat tccctcatac attcctgcaa gaacgcgggc cgtatctttg atgctctctt
     7201 ttttaccgat ctgggaaccg gtcgggtcaa gataggtggt tcccatgccg agatcatgtg
     7261 cggctacttc gaaggaacag cgggttctgg tgctggtttt ctcgaagatc agcgcaacgt
     7321 ttttaccgcg cagggtgtcc accggaatgc ctttcttttt cttatccttc agctctgcgg
     7381 acagatccag cagataggaa atctcttccg gggtgaaatc ctttaatgtc agaaaatcgc
     7441 gtccttttaa atccatggtg tgttcctcct taaatatatc ggtatgtaat gtatttttat
     7501 cgcttaagat ggtttctatt atgaaacagg ccggcgcatc tgtcaagggg tgaaatgtat
     7561 atttttacgt attttacttg tattattcat aatatatgta tttttataca tttttattct
     7621 gttattctta ccggcaacta cagaatctgc cagtggcagc tccctccgga tgcagagcaa
     7681 taaaaaattc tcccgtgtcc ggcatgatgc gtttcacggg agaatcctgc ttttttattt
     7741 ctgtccttct ttccctgccg ggcctttctg ctcttccacg attcccttga atgtggtagc
     7801 cagacttgcc agtccctgca gatcggacgg aacaacaatc ttcgttgctt ttccattcgc
     7861 cagaacacca agtgcctcga gactctttaa ggtaagaacc gcatcgtcag cacctgcctc
     7921 tttgatggca cggataccat cggcatttgc ctgctgtact ttgaggatcg cctccgcctg
     7981 gccttctgcc tcgcggattc gtttctcttt ttctgcctca gcacgaagaa tcgctgcctg
     8041 cttctctgcc tctgcatcca ggatcgcaga ctgtttttta ccttcggcaa ccaggatggt
     8101 cgattttttc tcaccttctg caatcaggat tgcttcacgg cgttcacgct ctgctttcat
     8161 ctgtttctcc atggcttcct gaatggctgc cggaggcatg atgtttttca gctcaacacg
     8221 gttgacttta atgccccacg ggtcggttgc gatatcgaga gctgcctgca tcttcgtgtt
     8281 gataatctca cgggatgtca gtgtttcgtc cagctccaga tctccgatga tgtttcgaag
     8341 tgtcgttgcg gtcaggtttt caatggccat cagcggattt acaacaccgt atgcgaacag
     8401 cttcggatcg gtaatctgga aaaatacgac cgtatcaatc tgcatcgtaa cgttatcttt
     8461 tgtaatgacc ggctgcggcg ggaaatccac cacctgctct tttaagttca cgcggattgc
     8521 cacgcgctcg ataaacggaa ccttaaaatg aagaccggtt gcccaggtgc ctttgtattt
     8581 tccaagactc tcaacaacgt aagcactggc ctgcggcacg atcttcacac acgatgctgc
     8641 aataaacagt acaatcacaa gaagaaaaat cattaaaatt cctgcaattc ctccaccact
     8701 catgtttcat ttcctccttc tttttctttg acctttacaa tcagcttgac gccttccaca
     8761 cggagcactt ccaccacggt ctctttcggg ataatgcctc cttcgctttc cgttctcgca
     8821 gtccaggtgt ttccgcccag cgataccttt ccggtgcctg ccaggttgga aatctcttct
     8881 gcgacgatcc cttccattcc aatcacgcgg tctacatttg tttttaaggt atccttattc
     8941 aggaaacgga cggctacagg acgtgtaaaa atcagaagta tcagcgatac aacaagaaag
     9001 cagaccatct gaattacaaa tccggctccg cacatagctg cgatcagagc aatcagggcg
     9061 ccgcccgcaa accagatggt tgccagacct aacgttgccg cctcaatcac cagcaaaacc
     9121 acaagcgcaa tcagccagta cactgcatat tccatgctcc tctctccttt ccgatttctt
     9181 ggctgttgga ttcatgatac tatatgagaa tgaaaatagc aagcggagag gtggaatatt
     9241 cagaaaaaag acaggatttt aagaactggt cgcacagaga cagcgcagaa aacccgatct
     9301 tcttattctt ccatcacatc ttttcatttt aaaacggcta caaccattct ttacacactc
     9361 ctatctgttg tgatgcagtt atagaaacga agaaatttgt aatgtttaaa gcgttcgaaa
     9421 aagcagatta cattttcata acgttttgca cctgtgtgaa gtgaaaggaa tggctataaa
     9481 aatgaaaaac tggaaaacag ctctttcaag aaggaaataa agaaaggaaa tataaagact
     9541 gattttttat ttataaacta tcaattccaa ggcttgacat ccatacaaaa acagctgcca
     9601 caacaaaata catatgcatc tgtataaaaa gtaccaaggt aaatgctaat aatttccaaa
     9661 acagcgattt aatctgcctt gtataacgaa tgcactcttt tagtgtcagt ttttgttcta
     9721 caaacgggtt tctgctgtat tgctttaata tcttcgccgc gacacgaatt tttttccctt
     9781 gatatgtcat ttcttctttg gcaagataca ttgcaccaat gagcagatga aagaaaatca
     9841 gatctccata ttctctggaa agatgctttt ctttcagaaa ctgttcaaaa tcagatgcaa
     9901 tcaaaatcca gtttctcatc agattctttt tcggttgata tgtcagggac tggagatttt
     9961 cacggtaaag aacgatgctc tgtgggacaa aagcatattt cggatggcat gcacagcatc
    10021 taaaattatg tgccttgtct tcggcgtaac tgtatggggg aatccacaaa tcgtgagact
    10081 ccagaaaaga tttgcggtag agttttcccc agtcgtaaga aagcgttcct ttttgaaaaa
    10141 atccatgaaa ccggaactct gcgctgtccg ggttttcttt ttccagatta ctgcagcatt
    10201 tcactttttc gccattcgcc ctccatctgc gatatgttcc gatcacaata tctgcctgct
    10261 ctctttctgc ccgtgcggca agggaacgta tggcagatga atcgtccagc ttgtcgtctg
    10321 catccaggaa gagaagatat tttcctgttg cttcttttat ccctgcattt ctggctgctc
    10381 cactgccttt attattctga tgaatcacac ggattcttgg atctgctgca gcaagttcct
    10441 cacacatttg attggaacga tctgttgaac cgtcattcac caaaacaatc tccaactctg
    10501 aatagtcctg ttttcgtacg ctctcaacac actccgtcag aaatttttcc gcctgataga
    10561 ccggaataat cacacttacc agtggataaa acatcttttt tctttcctct tctcctattc
    10621 tttgtaccca aaatatagcg gatttttaaa gaaacgtcaa ctgaactttg ctttcttttt
    10681 gttttcattt acaaatgtct cagcgtattg attcctccat tattctataa aacaagctat
    10741 ttctcatcca ctctttacac cacagtatat gttgtgctaa agttataaaa gggtcaaaca
    10801 tttgcacacc cattcctatt tttcctgaaa acatttttcg gatatattct gcacgttgga
    10861 cattactggc agtattccta cttagcagtt gcatcaatat atggctttgg cgaacagatc
    10921 ataaaaaaat acgttttatc tgtctggatg cttttgtcat gttgttaatc atgaatcttg
    10981 gaaatggata tatccattat taccagatgc tatcttaaat aaaataccag aaaatgagcg
    11041 gtccagtgta tataattatg agacaagttc ccaatggctg ctgtctggac ttgaacctta
    11101 tgacaaatac tgtattacag cagaccattt ttcgatgctt tcaaatgaaa tttttcagca
    11161 tattcaaaat ttattcgagc acagagagcc tatgtacata gtaacagggc gcaatgtgca
    11221 gattcaaaac caaattgtac aaacatatct ggaaaaagat tatactgaat tgtataccaa
    11281 caacatttat acattatatc aaaaaattcc ttaaacacta aataaatcgt tctcactatt
    11341 aaagtaaaga aaaaaatata tcaacttcta caaagaaaat tgaacaactt tgacaatgta
    11401 aactgatttt ttgtactgtt tcgttatcga aagtatactg ttttttttgg tacaatatac
    11461 gaaaggatcg ttacaaatga aaaaacagat cattgatttt gtcatcacat gggtggacgg
    11521 atcagacccc ttatggtttc gccaaaaaca gaacgccaaa aaaaagcaag gagtttctgt
    11581 tgatatcgac gaccgtattg aacgctatcg cgactgggaa attctaaagt atctatttcg
    11641 ttcgatagaa aactttgctc catgggtaaa taacgtatat tttgtaacat gcggtcatct
    11701 gccggcgtgg ctcaatacag aatgtccaaa attgcagatt gtttcacaca aagattttat
    11761 tcccgaaaaa tatctcccca cctttaactc gcattccatt gagtggaatt tccaccggat
    11821 aaaaaatctg tcggaacact ttgtttattt taatgatgat atgtttttgc tgcatccaat
    11881 aaagccaacc gatttttttg aaaacggaaa acccgttgat atgcttgccc tgcagcccga
    11941 tgttgcaaac gtagaagata ccattatgcc atatatctat ctgaacaata ctatgctgct
    12001 tgccaagtac tttgacaaac gggaaaacat gaaaaagcag ccaggtgcct attttcacat
    12061 cggatatcct cccatgtact ttgggtataa tcttctggaa atggcatttc cacgatttac
    12121 cggtttttac accgttcatg gcccgtctcc tttgaaaaag gagacttatc gtttcttctg
    12181 ggaaaaggaa gaagaattgt tagacggtgt ctgcagccac ccatttcgtc ataaagatga
    12241 tgtgagccaa tatgttctgc gggaatatca gaaactgagt ggtgatttcg ttccaagaaa
    12301 cacgcacaag ttctgtaaat acttcaattt aaaaccggaa aacccggaac tggtaaagac
    12361 aatcacaaag cagactgcaa agtgcatctg catcaatgac agcaattttg aaatcccata
    12421 tgaacatgta aaacgggaac tgaacgatgc cttttccaaa ctttttccgc agaaatcaca
    12481 gtttgaaaag taaattttgt tccatattca gaggcggaaa tttatctgca tctattttgg
    12541 cattacgtta tcggaacgtt cttgtttttt aatcgaaata gtaacatttt taccccaaat
    12601 ctattgacat ttgcgggatt ttcctcctat aataagaatt gtaaccaagg atgattttgc
    12661 aagaaaagga cgtgaatata tatgactaaa aattacacaa aaccaatcgt tacaattgat
    12721 tctggtatgg cagaaggtgt ttatgcggca agtggtgcaa caaaaggaac tctgaacgtt
    12781 acttattatg gcgtatggga tcgttggggc acaaatggtg gtaaaggact tgcacaggca
    12841 agctggtctg atctggatgg aaccatcaca ttgaatatta atttcaatga tactgttgat
    12901 tcaatcgaga cagatgatgc ctccgtacag aaatcctggt ctggaaaaac tgccaccctc
    12961 acttttgcaa gcacagcaac caatccgctg accattggta ttcatctgaa ccatggaacc
    13021 agcatcgacg atctgcagct cactggattt gactactcag tgaactaaaa aatgaaccat
    13081 gtatgtgaac catatctttt cgcttcgttt gaaaagatat ggttcacaga aaaaagcagc
    13141 cagaatggcc gcggaagggg tgcagaaaat gagtaaatat gtaaaacctg taatttctct
    13201 ggatgaagga atggctgaag gtgtttatgc ggcaagtgga tgttatactg catctgcata
    13261 tattcaccag actccagaaa acggacgtgg cgattaccgt attcaggtaa atggaaagca
    13321 taatgctgat cataccaagg aagcccaggt tctgactatc agtttcaacc agaatgttac
    13381 ttatgtttct ggcggcgcag gacttatttc cggtaacgaa accactacac tgaaagtaaa
    13441 actggcttat catcagaatc caaccgataa tatcggtttc ggtgacttgg ttgttacttc
    13501 cgatgcaggt cttgcaatta ctggagtaag cattaccgat taatggatca cttcccgtat
    13561 tctaaaaaat ggaatgcctt ttggcattcc attttttatt cgcttgt
//
LOCUS       DAOMUF010000040        13297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1046_length_13297_cov_3.475683, whole genome shotgun sequence.
ACCESSION   DAOMUF010000040 DAOMUF010000000
VERSION     DAOMUF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 13297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13297
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1046_length_13297_cov_3.475683"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..2112)
                     /locus_tag="PLN77_03880"
     CDS             complement(133..2112)
                     /locus_tag="PLN77_03880"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HRM43455.1"
                     /translation="MSKKGLKFGIASAAVIGAGAAVLQAKNKSEAKKAAETTTVTSEP
                     KNDYRNTERGKDKKNSKGIYYTNGNYEAFARPEKPEGVDEKSAYLVGSGLAALAAACF
                     LVRDGQMKGDHIHILEAMDIAGGACDGIYDPTRGYIMRGGREMENHFECLWDLFHSIP
                     SIETPGVSVLDEYYWLNKKDPNYSLCRATKNRGEDAHTDGKFNLSQKGCMEIMKLFMT
                     RDEDLYDKTIEDVFDDEVFNSTFWLYWRTMFAFENWHSALEMKLYFQRFIHHIGGLPD
                     FSALKFTKYNQYESLILPMKKYLEDAGVDFQFNTEVTNVIFEINDGKKVAKAIECKVN
                     GVEKGIVLTENDLVFVTNGSCTEGTIYGDQNHAPNGDAEVRTSGCWSLWKNIAKQDPS
                     FGHPEKFCSDIAKTNWESATITTLDNKIIPYITNICKRDPRTGKVVTGGIVSCQDSKW
                     LLSWTINRQGQFKEQDKDKVCVWVYSLFTDVPGDYVKKPMKECTGKEITEEWLYHLGV
                     PVDQIPELAENSAVCVPTMMPYITAFFMPRRKGDRPDVIPDGCVNFAFLGQFAETPRD
                     TIFTTEYSVRTAMEAVYGLLGVDRGVPEVWGSVYDIRDLLDSSVKLMDGKSPLEIELP
                     GPLNLLKKPLLKLVDGNVVGDLLKEHGIIKEDMVK"
     gene            complement(2332..2898)
                     /locus_tag="PLN77_03885"
     CDS             complement(2332..2898)
                     /locus_tag="PLN77_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HRM43456.1"
                     /translation="MTNATKAALEASLKKLLLKKPLDKITINDLTTDCGISRMAFYYH
                     FKDIYDLVEWSCLEDATQALQGKKTYETWQEGLQQIFEAVLENKPFIMNVYHSVSREQ
                     IETYLFHLTHDLLYGVVQEKAKGTGISEADQSFIADFYKYSFTGVMLDWIKDGMKVDY
                     HMIAEKMHRTMEGNVVNSIRNFEKRNKI"
     gene            3242..5317
                     /gene="htpG"
                     /locus_tag="PLN77_03890"
     CDS             3242..5317
                     /gene="htpG"
                     /locus_tag="PLN77_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRM43457.1"
                     /translation="MSAKHGNLSINSDNIFPIIKKWMYSDHDIFVRELVSNGCDAITK
                     LKKLDGMGEYTLPDDYKAAIQVIVNPDEKTLKFIDNGIGMTADEVEEYITQIAFSGAT
                     EFLEKYKDKTNSDQMIGHFGLGFYSAFMVADEVHIDSLSYKEGSTPVHWTCDGSTEYD
                     MSEGSKTTVGTEITLFLNEDCLEFANEYRVREVLEKYCSFMPVEIFLSKANAPQEYET
                     IDESELKDDDVVVEHIHEDAKYEEKEKEDGTKEQVEVSPAKEKVKINKRPVSISDIHP
                     LWTKHPNECSDEDYKEFYRKVFNDYREPLFWIHLNMDYPFNLKGILYFPRINTEYDSI
                     EGTIKLYNNQVFIADNIKEVIPEYLMLLKGVIDCPDLPLNVSRSALQNDGFVKKISEY
                     ISKKFADKLSGMCKTDRENYEKYWDDINPFIKYGCIKDEKFSKKMMDYILFKNIDGKY
                     LTLEDCINENKKEEAPAENTEEKAETTENTENTENADEKKEPEKTTVYYVTDEVQQSQ
                     YINMFRSQGMDAVILKHNIDSAFITHAERYNEHVTFQRIDADLTNDMKDESGEDLTDA
                     TNTLTDLFRKVLDKKDLTVKVENLKDENVSSMVTLSEESRRMQDMMKMYGMTGMDPSM
                     FGGQETLILNAKHPLVQYILKNGENDNTSLFCEQLYDLAMISHKPLSPDEMTKFVSRS
                     NQIMMLLAK"
     gene            5307..6827
                     /locus_tag="PLN77_03895"
     CDS             5307..6827
                     /locus_tag="PLN77_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRM43458.1"
                     /translation="MQNDKAQAQQNAPLIKHAGFLEFTRCPACFFLLFYFTISDPDGR
                     IILFALLKNKENHTKGLVFISMKKGKPSALLPIAVFLVFYLGLGIYSEYILKIPMGFY
                     NIPIVVAFLAAILVACLQNRSVPFEEKLALMGQGIGDKNIITMLLIFLAAGTFVGVVG
                     RSSAESVAYFMLSWIPAKYAVAVLFVVACFVSTAMGTSVGTITLLVPIAAAVAETSGF
                     SLALCVASVMGGSMFGDNLSFISDTTIAACNGQGCEMKDKFRENFWIALPAALAALAL
                     ILVISFRQAPGTPIVHEYHLLQMVPYLLVLIGGIAGIQVFVVLLIGIASGAVIMLGTG
                     QTTLWDMLSSMGSGTSGMFETCMVAILVAAMCALIRENGGFTVLLRAIHKIFRGKKGG
                     QLGVGILVALLDIATANNTVAIVMANPIAKQMSEDYGITPRKTASLLDTFSCISQGIL
                     PYGAQMLVAISAVHELGYELSAFEIMANLFYPGMLLVSSLIFIFVLPDRKNAESNA"
     gene            7051..7422
                     /locus_tag="PLN77_03900"
     CDS             7051..7422
                     /locus_tag="PLN77_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HRM43459.1"
                     /translation="MKLLRQFLVIMAVSFVGEILHAVLPLPIPASIYGLVLMLALLMT
                     GALKLDAVEDAGKFMIEIMPVMFIPAGVGLMESWGELKAVLVPVLVITLVSTIVVMVV
                     SGRVTQAVIRLEKKHKGGDAK"
     gene            7419..8111
                     /locus_tag="PLN77_03905"
     CDS             7419..8111
                     /locus_tag="PLN77_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRM43460.1"
                     /translation="MTNMLCESAFFGVVISLLAYELGMLLKKKFGLPIFNPLLISIAV
                     VIVFLAVFDIDYQNYNDGAKYLSYLLTPATVCLAIPLYEQMEALKKNIKAILAGILSG
                     VLTSLTVVLALALIFNLNHKMYVTLLPKSITTAIGMGVSEELGGAVTITVAVIIITGV
                     LGNMLAETLCKLFHIEEPIAKGISIGSASHAIGTAKAMEMGDVEGAMSSLSIAVAGIL
                     TVVGASIYAMFI"
     gene            complement(8212..9348)
                     /locus_tag="PLN77_03910"
     CDS             complement(8212..9348)
                     /locus_tag="PLN77_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRM43461.1"
                     /translation="MNEKSITTITLKKINKKSVYQYIYEHRQTAKQQIVQDLTMGLST
                     VSQNLNVLEKEGLISRDGFFESTGGRKAQVIQIVPEYRIAVGVGILKNMFHLAAVNLY
                     GEAIAEETVAVPFAQSDAYYDGIASSLESFLDRNGYDREKILGVSIATQGIPAPDGES
                     VLYGEILHNTDMKLADFSTRIPYPCRLEHDSKAAASLELWNHPEIESAVVFLLNRNLG
                     GAVITNHKVHQGLSMHSGTLEHICIDPEGPECYCGQKGCLETYCSANALEAAAQMPVK
                     EFFPALRAGNDARLTEIWDAYLSHLAYAMKTANLLLDGAVIVSGYLAPYFKEEDCRTL
                     LEKVNAASPFPLVREQLLVGTHGQYTPAIGAALLFIEEFLQSEF"
     gene            complement(9367..9876)
                     /gene="ilvN"
                     /locus_tag="PLN77_03915"
     CDS             complement(9367..9876)
                     /gene="ilvN"
                     /locus_tag="PLN77_03915"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRM43462.1"
                     /translation="MNKGMKKRWISLYVENQVGVLSKISGLFSAKSYNLDSLTVGTTE
                     DPTVSRMTIATVSDDETFEQIKKQLNRMVEVIKVIDFTDVFVRMKEILYIKVFNCSAA
                     DKVELFQIAETFKAKVIDYGKDSLLLEFVQTATKNDAVVKLMKEEFGKIEVVRGGSVG
                     IESINVMER"
     gene            complement(9889..11607)
                     /gene="ilvB"
                     /locus_tag="PLN77_03920"
     CDS             complement(9889..11607)
                     /gene="ilvB"
                     /locus_tag="PLN77_03920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRM43463.1"
                     /translation="MKQITGNKLLVKALKEEGVEYLFGYPGACTIDISDELYKQDEIK
                     IVLPRHEQALVHEADAYARTTGKVGVCLVTSGPGATNLVTGLATANYDSVPLVCFTGQ
                     VARHLIGNDAFQEVDIVGITRSITKYGVTVRNREDLGRIIKEAFYIARTGKPGPVLID
                     LPKDVMAELGSAEYPKNVNIRGYKPNTSVHIGQLKRALKMLAKAKRPLFLAGGGVNIA
                     HAQEVFREVVEKTQVPVVTTVMGRGAISTKHPLFIGNLGMHGAYAANMAVSECDLLFS
                     IGTRFNDRITGKLHEFAPHAQIVHIDIDTASISRNIHVDIPIVADAKEAITKMAEYVE
                     PCSTSKWLAQIDAWKNEHPLTMRPNGVMGPLDIFNAINEQFDDAIIVTDVGQHQMLTS
                     QYVDITENRQIIMSGGLGTMGYGLPGAIGAKIGNPDKPVIAISGDGGMQMNIQELATA
                     VLEELPVILCIFNNEYLGMVRQWQKLFYGKRYSMTNLRAGALSRRTDGEEYPEYTPNF
                     IKLAESYGAKGIRVMKKEDIAAAFEEAKKNTKTPTIIEFIIDPEEMVYPMIKPGGTLE
                     DMIMDC"
     misc_binding    complement(11679..11954)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12099..13220
                     /locus_tag="PLN77_03925"
     CDS             12099..13220
                     /locus_tag="PLN77_03925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRM43464.1"
                     /translation="MMITYTQEELSSIVHAFAFPGTITDWTPHGNGHINDTFLVRSTE
                     NGVLRKSILQRMNRTVFHNPTELMQNIAQVTSFLRKKITAAGGDPFRETLNLIPTTDD
                     AWFFVDERGEFWRAFLFISDASCYDAVTDPKLFYETGNAFGRFQQMLSDFPSASLFET
                     IPDFHNTPLRYQALLQAAQADPKGRLKNVSDEMAFFLDHADDYGVAERMKASGELPLR
                     VTHNDTKLNNIMIDDKTGEGICVIDLDTIMPGLSIFDFGDSIRFGANTAAEDEQDVSK
                     VSLSLPLFESYTKGFLAGCAGSLTRAEVEMLPMGAKLMTLECGIRFLADYLMGDTYFK
                     TAYPEHNLVRARTQIALVADMEEKWSEMENIVARASFSH"
BASE COUNT         3084 a         3490 c         3160 g         3563 t
ORIGIN      
        1 ctgcagtgct gaattttttg cattattttc tggaattgct ataattgttt gagaccatac
       61 atagcaacgc aataagacct gcgccgtttg aaagcgcagg tcttattgca tttcttactt
      121 attttctatt tcttatttca ccatatcctc tttgataatt ccatgctctt ttaagagatc
      181 tccaactaca tttccatcta ccagtttcag cagaggtttt ttcagcaggt tgagcggtcc
      241 aggaagctcg atttccagcg gggattttcc atccatcagt ttcacggagc tgtccagcag
      301 gtcgcggata tcgtaaacgc tgccccatac ttccggcacg ccgcggtcta cgccgaggag
      361 tccgtaaaca gcttccattg cggtacgaac ggaatactct gtcgtgaaga tggtatctct
      421 tggtgtctca gcgaactgtc cgaggaatgc gaagttgacg cagccatccg ggatgacatc
      481 cgggcggtcg ccctttcttc ttggcataaa gaatgcggtg atgtacggca tcatggtcgg
      541 tacgcagaca gcgctgttct ctgccagctc cggaatctgg tcaaccggca cgccgaggtg
      601 gtacagccac tcttcggtga tctctttacc agtgcactct ttcatcggtt tcttcacata
      661 gtcgccaggt acgtcggtga acaggctgta aacccatacg cataccttat ctttatcctg
      721 ctctttgaac tgtccctgac ggttgatggt ccagcttaaa agccacttgg aatcctggca
      781 gcttacgata ccgccggtaa caacctttcc ggttctcgga tcacgtttgc agatattggt
      841 gatatacgga atgatcttat tgtcgagagt ggtaattgtt gcggattccc agtttgtctt
      901 tgcaatatcg gagcagaatt tctccggatg tccaaaggac ggatcctgtt ttgcaatgtt
      961 tttccacagg ctccagcagc cgctcgtgcg gacctctgca tcaccgttcg gtgcatggtt
     1021 ctgatcgccg tagatcgtgc cttcggtgca gcttccgttg gtaacgaaga cgaggtcatt
     1081 ctcggtgaga acgatgcctt tctcgacgcc gtttactttg cactcgatgg cttttgctac
     1141 tttctttcca tcgttgattt caaagataac gttggttact tcggtattga actggaagtc
     1201 tacgccggca tcctcgaggt atttcttcat cggaagaatc agggattcat actggttgta
     1261 cttcgtgaat ttcagtgcgg agaagtccgg cagtccgccg atgtggtgaa tgaaccgctg
     1321 gaagtacagc ttcatctcca gtgcactgtg ccagttttcg aatgcgaaca tcgttctcca
     1381 gtacagccag aaggtggaat tgaagacttc atcatcgaaa acatcctcga tcgtcttgtc
     1441 gtacagatcc tcgtctcttg tcatgaagag tttcatgatc tccatgcagc ctttctggct
     1501 caggttgaac ttgccgtcgg tatgggcatc ctcgccgcgg ttcttcgttg cacggcacag
     1561 ggagtagttc gggtcttttt tgttcagcca gtaatactca tcaagaacgg atacaccagg
     1621 tgtctcaatt gacgggatgc tgtggaacag atcccacagg cactcgaagt ggttttccat
     1681 ctcacggccg cctctcatga tgtagccgcg ggtcggatcg tagataccgt cgcaggcacc
     1741 gccagcgatg tccatggctt ccagaatgtg gatgtgatcg cctttcatct ggccgtctct
     1801 tacgaggaag cacgctgctg cgagggctgc gaggccgctt cctacgaggt atgcgctctt
     1861 ctcatcgacg ccttccggtt tttccggtct tgcgaaggct tcgtagtttc cgttggtgta
     1921 ataaatgcct ttgctgtttt tcttatcttt tccgcgctcg gtgttgcggt aatcattctt
     1981 tggttctgat gttacagtgg ttgtctccgc tgcttttttt gcttcactct tgttttttgc
     2041 ctgcagcact gctgctccag ctccgatgac tgctgctgat gcgattccga attttaatcc
     2101 tttttttgac atagacgttc atcctttctt ttctaaatac gactgccgga atgttttttc
     2161 gaatttccgg tttgtatagg ttatgctgct ttctttgttt tacaagtttc tatttgtgtt
     2221 gccattgtct gttgacaatt tgaatcttac ttcatttttc cccgcttttc catttacaaa
     2281 acagggcgga tgattaaatt tttatcatcc gccctgtttt gtaaagtgta actatatttt
     2341 atttcttttt tcaaaatttc ggatggaatt gacgacattt ccttccatcg tgcggtgcat
     2401 tttttccgca atcatatggt aatccacttt cattccgtct ttgatccagt cgagcatgac
     2461 accggtaaag ctatacttat agaaatctgc gataaaactc tgatccgcct cggagatgcc
     2521 ggttcccttt gctttttcct gtacgacacc gtacagcaga tcgtgcgtca gatgaaagag
     2581 atatgtttcg atctgctccc ggcttacaga gtgatagaca ttcataataa atggtttatt
     2641 ctcgagcact gcctcaaaaa tctgctgcag gccttcctgc catgtctcat acgttttctt
     2701 cccctgcagc gcctgcgtcg catcctccag gcacgaccat tcgacaagat cgtagatatc
     2761 cttaaaatga taataaaacg ccatccggct gatgccgcag tctgtcgtca gatcgttgat
     2821 cgtaattttg tccagcggct ttttcaaaag cagctttttt aatgacgctt ccagcgccgc
     2881 ctttgtcgca tttgtcatgt atttcctcca tttttctttt atgtaccctc ggaatctttc
     2941 tgttcgaaac gcttctatgc tttttcattg tagcatatcc attctccaga aacaactgcg
     3001 cagcccttct gatcagactt tacaaaaagt ttagaaagta ccgtaatacc agtaaattca
     3061 atactttccg tcgttctgtt agcactcttt tttctcgagt gctaattttc ccttgacttt
     3121 tccctgtgcc agtattacta taactatcag agcagtaaaa aagctgcaga catctaaaaa
     3181 agcatgatat tcccgctttg ctggatcaaa atatttttat aagaaaagga gtgtgtttct
     3241 catgagtgcg aaacatggaa atctttcaat caacagcgac aatattttcc cgattatcaa
     3301 aaaatggatg tattccgacc acgatatctt tgtccgtgag ctcgtttcca acggctgcga
     3361 tgccatcacc aaactgaaaa aactcgacgg catgggcgaa tatactctgc cggatgacta
     3421 caaagcagcc attcaggtga ttgtaaaccc ggatgagaaa accttaaaat tcatcgataa
     3481 cggtatcggt atgaccgcag acgaagtcga agaatatatt acccagatcg ccttctccgg
     3541 cgctaccgaa ttcctggaga aatacaaaga caaaacgaat tcagatcaga tgatcggcca
     3601 cttcggtctt ggtttctact ctgctttcat ggtagcagat gaagttcaca tcgattctct
     3661 ttcctataaa gaaggatcca ccccggtaca ctggacctgt gacggaagca ccgagtacga
     3721 tatgtccgag ggcagcaaga caactgttgg taccgaaatc actctgttcc tgaacgagga
     3781 ctgcctggaa ttcgcaaacg aataccgtgt ccgcgaagtt ctggaaaaat actgctcctt
     3841 catgccggtt gaaattttcc tgtccaaagc aaatgcgccg caggaatacg agaccatcga
     3901 tgaaagcgaa ttaaaagacg acgacgttgt cgtagagcac atccacgagg acgcaaaata
     3961 cgaagagaaa gaaaaagaag acggaacgaa agagcaggtt gaagtttccc cggccaaaga
     4021 gaaagtaaaa atcaacaaac gcccggtttc catcagcgat atccacccgc tgtggaccaa
     4081 acatccaaac gagtgcagcg atgaggatta taaagaattc taccgcaaag tcttcaacga
     4141 ctaccgcgag ccgctgttct ggatccacct gaacatggac tatccgttca acctcaaagg
     4201 tatcctctac ttcccgcgta tcaacaccga gtacgattcc atcgagggaa ccatcaagct
     4261 gtacaacaac caggtattta ttgccgacaa catcaaagaa gtcattccgg aatacctgat
     4321 gctcttaaaa ggcgtcatcg actgcccgga tctgccgctg aacgtatccc gaagcgcact
     4381 gcagaacgac ggatttgtca aaaagatctc cgagtacatc tccaagaaat tcgctgacaa
     4441 gctctccggc atgtgcaaaa ccgaccgtga gaattacgag aaatactggg atgatatcaa
     4501 cccattcatc aaatacggat gcatcaagga cgagaaattc tccaagaaga tgatggacta
     4561 tattctgttc aagaacatcg acggaaaata tctgactctt gaggactgca tcaacgagaa
     4621 taagaaagaa gaagctcctg cagagaacac cgaggagaag gctgaaacta cggagaatac
     4681 cgaaaatacg gaaaacgctg acgagaagaa agagccggaa aaaacgactg tttactacgt
     4741 caccgacgaa gttcagcaga gccagtacat caacatgttc cgcagccagg gcatggatgc
     4801 tgtcatcctg aaacacaaca tcgactccgc tttcatcacc catgcagagc gttacaacga
     4861 gcatgtaacc ttccagcgca tcgatgcaga tctgaccaac gatatgaaag acgagtccgg
     4921 tgaggatctg accgacgcta caaatactct gaccgatctc ttccgcaaag tcctcgacaa
     4981 gaaagatctg accgtcaaag ttgagaacct gaaagacgaa aatgtatcct ccatggtaac
     5041 gctttccgag gagagccgcc ggatgcagga tatgatgaag atgtatggca tgaccggcat
     5101 ggatccgtcc atgttcggcg gacaggaaac cctgatcctg aatgcaaaac atccgctcgt
     5161 tcagtatatt ctgaagaatg gcgagaacga caacacatcc ctgttctgcg agcagctgta
     5221 tgatctggca atgatcagcc acaaaccgct ctctccggac gagatgacaa aattcgtaag
     5281 cagaagcaat cagattatga tgctccttgc aaaatgataa agcacaggca cagcaaaacg
     5341 cacctcttat aaaacacgcc ggatttctgg aatttaccag atgtccggcg tgttttttcc
     5401 tgctttttta ctttacgatt tctgatccag atggtagaat aattctattc gccctattga
     5461 aaaataagga aaatcacacg aaaggtctgg tatttatttc tatgaaaaaa ggaaagccat
     5521 ccgccctgct cccgatcgcc gtttttctgg tgttttatct ggggctcggc atttattccg
     5581 agtatatttt aaaaattccg atgggcttct acaacatccc catcgtcgtc gcgtttctgg
     5641 cagcgatcct cgtcgcctgt ctgcagaacc gcagtgtccc gtttgaagaa aagctcgccc
     5701 tcatgggaca gggcatcggg gacaaaaaca tcatcacaat gctgctgatc ttcctcgccg
     5761 caggcacgtt tgtcggcgtt gtcggaagga gcagtgcaga gagcgttgcc tatttcatgc
     5821 tctcctggat tccggcaaag tatgccgtcg ctgtgctctt tgtcgttgcc tgcttcgtct
     5881 ccacggccat gggaacctcc gtcggaacga tcacgcttct cgtgccgatc gccgctgctg
     5941 ttgccgagac ctccggcttt tcgctggcgc tctgcgtcgc ctccgtcatg ggcggatcca
     6001 tgttcggcga caacctctct ttcatctccg acacgacaat cgccgcctgc aacggacagg
     6061 gctgcgagat gaaagacaag ttccgcgaaa acttctggat cgctctcccg gctgctcttg
     6121 ccgcgctggc gctgatcctt gtaatctcct tccgacaggc gccgggcaca ccgatcgtcc
     6181 acgaatacca tctgctgcag atggttccgt acctgctcgt gctgatcggc ggcatcgcgg
     6241 gcattcaggt gtttgtcgta ctccttatcg gtattgcttc cggtgccgtt atcatgctcg
     6301 gtacgggaca gacgacgctc tgggatatgc tctccagcat gggaagcggc acttcgggta
     6361 tgtttgagac atgtatggtt gcgattcttg tcgctgcgat gtgcgcgctg attcgggaaa
     6421 acggcggctt tactgtcctg cttcgtgcca ttcacaaaat cttccgcgga aaaaagggcg
     6481 gccagctcgg tgtcggcatt ctcgtcgcgc tgctcgacat tgcgaccgca aacaacaccg
     6541 tcgcgatcgt catggcaaac ccgatcgcaa agcagatgtc cgaggactac ggcatcaccc
     6601 cacggaaaac ggcatcgctc ctcgatacct tctcctgcat ctcacagggc atcctgccgt
     6661 acggcgcgca gatgctcgtg gcgatttccg cggtgcatga actgggatat gagctctccg
     6721 cctttgaaat catggcaaac ctcttctatc cgggcatgct gcttgtcagt tcgctgatct
     6781 ttatcttcgt tctgccggat cggaaaaatg ccgaaagcaa tgcttaaaaa gttccgtatc
     6841 ggatcaactc aaaaaattat gttaaaatgc ccaccgattt cactttttcg tttgatcggc
     6901 gggcattttt ttgctttttt ttgcaaaaat tgccaaaatt gcagtaacat ttacggttga
     6961 aattctttta tgcgggtagt ataatcgggg tcgtgacatt gagaagtaat gatgttatgt
     7021 gggttatggg gagaaaggac aacagacaaa gtgaaattat taagacagtt ccttgtaatt
     7081 atggcggttt cgttcgtcgg agaaatcctg cacgccgtgc tgccgcttcc gattccggca
     7141 agtatctacg gccttgtgct gatgctggcg cttctgatga cgggggcatt aaaacttgat
     7201 gccgtcgagg acgcaggcaa gtttatgatt gagatcatgc cggtcatgtt cattccggcg
     7261 ggcgtcggcc tgatggaatc ctggggtgaa ttaaaagccg ttctcgttcc ggttctcgtg
     7321 attacgctcg tcagcaccat cgtcgttatg gttgtgtccg gacgcgtgac acaggcggtc
     7381 atccggctgg aaaagaaaca caaaggaggg gacgcaaaat gacaaatatg ctctgtgaat
     7441 ctgcattttt cggcgttgtc atcagccttc tggcgtatga gctcggcatg ctgttaaaga
     7501 aaaaatttgg gcttccgatt ttcaatccgc tgctgatttc cattgcagtc gtcattgtat
     7561 tccttgctgt tttcgatatc gattatcaga attataatga cggtgcaaaa tacttgagct
     7621 atctgctgac tcctgcaacg gtctgccttg cgattccgct gtatgagcag atggaggcat
     7681 tgaaaaagaa tatcaaagcc atcctcgccg gtattctctc cggcgtgctg acgagcctta
     7741 ccgtggttct cgcgctggcg ctgatcttca atctgaatca taaaatgtat gtaacactgc
     7801 tgccgaaatc catcacaacg gccatcggca tgggtgtttc ggaagagctc ggcggtgcgg
     7861 tgacgatcac ggttgccgtt atcatcatca cgggtgtact gggaaatatg ctggcggaga
     7921 cgctctgcaa gctgtttcac attgaggagc cgattgcaaa gggcatcagt atcggatcgg
     7981 cctcccatgc gatcggtacg gcgaaggcca tggagatggg cgatgtcgag ggcgcgatga
     8041 gcagcctttc gattgcagtt gcaggtattc tgactgtggt cggagcgtcg atttatgcga
     8101 tgtttatcta aaaacgaaca ctgataaaaa acagaaaaaa tccgggaatg gaaagcaatg
     8161 taaaactttc cgttcccgga ttttctatcc ctgaaattct ataactaaaa attaaaattc
     8221 cgactgcaga aactcttcaa taaaaagaag cgccgcgccg atcgccggcg tgtactggcc
     8281 gtgtgtgccg accagaagct gctcccgcac aagcggaaac gggctggctg cattgacttt
     8341 ttcaagcagt gtccggcagt cttcttcttt gaaatacggc gcaaggtaac cgctcacaat
     8401 caccgctccg tcgagcagca gatttgctgt cttcattgca taggcaaggt gggacaggta
     8461 cgcatcccag atttccgtaa gacgcgcatc gtttcctgcc cgcagcgccg ggaaaaactc
     8521 tttgaccggc atctgtgccg ccgcttccag cgcattcgcg gaacaatacg tctcaaggca
     8581 ccctttctgt ccgcagtagc actccggccc ttccgggtcg atgcagatat gctcgagcgt
     8641 tccgctgtgc atcgaaagcc cctgatgcac cttgtgattc gtgatcaccg ccccgcccag
     8701 gttgcggttc agcaggaaga ccaccgcact ttcaatctcc gggtgattcc acagttccag
     8761 ggacgccgcc gctttcgaat cgtgctcgag gcggcacgga tacggaatcc ttgtggaaaa
     8821 gtcggcaagc ttcatatccg tattgtggag aatctctccg taaagcaccg actccccgtc
     8881 cggcgccggg attccctgcg tcgcaattga tacaccgagg atcttttccc tgtcataccc
     8941 gttacggtca aggaatgatt ccagagatga tgcaatcccg tcataatacg catcactctg
     9001 cgcaaacggc acagcgaccg tttcctctgc gatcgcctcc ccgtagagat tgaccgcagc
     9061 caggtgaaac atatttttca gaatgccgac accgaccgca atccggtact ccggcacgat
     9121 ctggatcacc tgggctttcc ggccgccggt cgactcgaaa aatccgtccc gcgaaatcag
     9181 cccctccttc tcgagcacgt tgaggttctg gctcaccgtg gaaagcccca tcgtcagatc
     9241 ctgcacaatc tgctgcttcg ccgtctggcg gtgctcatag atgtactgat acacactttt
     9301 cttattaatc ttttttaacg taattgtggt aatactcttt tcgttcacgc aaaaacgtcc
     9361 tctcttttac cgctccatca cattgatcga ctcgatgccg acgcttccgc cgcgcacgac
     9421 ttcgatcttg ccgaattcct ctttcatcag cttcacaact gcatcgtttt tcgtcgccgt
     9481 ctggacaaac tcgagcagca ggctgtcttt tccgtagtcg atgaccttcg ccttgaacgt
     9541 ctcagcgatc tggaacagct cgactttgtc agctgcgctg cagttgaata ccttgatata
     9601 cagaatctct ttcatgcgca caaacacgtc ggtgaagtcg atgactttga tgacctcgac
     9661 catgcggttc agctgttttt tgatctgctc aaacgtctca tcgtcactga ccgtggcgat
     9721 cgtcatacgc gatacggtcg ggtcctcggt cgttccgacg gtcaggctgt ccaagttata
     9781 gctttttgcg gaaaaaagcc cggaaatctt ggaaaggaca ccaacctggt tttctacgta
     9841 cagtgaaatc catctttttt tcattccctt attcatcgcg ctctcctcct aacagtccat
     9901 aatcatatct tcgagcgttc cgcccggctt gatcatcggg taaaccatct cctccggatc
     9961 gatgataaat tcgatgatcg tcggagtctt ggtgtttttc ttcgcctctt caaacgcagc
    10021 cgcgatatcc tctttcttca tgacacggat gccttttgcg ccgtaactct cagccaattt
    10081 gataaaattc ggcgtgtatt ccgggtactc ttctccgtct gttctgcggg aaagtgcacc
    10141 ggcgcgcaga tttgtcatgc tgtaccgttt gccgtaaaac agcttctgcc actggcgtac
    10201 catgccgagg tattcattat tgaagataca caggatgacc ggcagctcct cgagaacggc
    10261 agtcgccagc tcctgaatat tcatctgcat accgccgtca ccggaaatcg cgatgaccgg
    10321 tttgtccggg ttgccgattt ttgctccgat cgcgcccgga aggccgtatc ccatcgttcc
    10381 aaggccgccg gacatgatga tctggcggtt ttcggtaatg tcgacgtact gacttgtcag
    10441 catctggtgc tgtccgacgt cggtgacgat gatcgcatcg tcaaactgct cgttgatcgc
    10501 gttgaaaata tcgagcggtc ccatcacgcc gttcgggcgc atcgtcagcg gatgctcgtt
    10561 cttccaggca tcgatctggg caagccattt cgatgtggag catggctcta cgtactctgc
    10621 catcttcgtg atcgcctctt ttgcatcggc tacgatcggg atatcgacat ggatgtttcg
    10681 agagatcgaa gcggtgtcta tgtcgatgtg gacgatctgc gcgtgcgggg caaattcatg
    10741 cagctttccg gtgatacggt cattgaaacg tgttccgatc gagaacagca gatcgcactc
    10801 gctgactgcc atattcgccg catacgcgcc gtgcatgcca aggtttccga tgaacagcgg
    10861 atgtttcgtg gagatcgcgc cgcgtcccat aacggttgtg acaaccggca cctgtgtttt
    10921 ctcgaccact tcgcggaaca cttcctgcgc atgtgcgatg ttgacgccgc ctccggcaag
    10981 gaacagcggg cgttttgctt ttgcgagcat tttcagcgca cgttttaact ggccgatgtg
    11041 tacgctggtg ttcggtttgt agccgcggat gttgacgttc ttcggatact ctgcgctgcc
    11101 gagctctgcc atgacatctt ttggaaggtc aatgagaacc gggcccggtt ttccggtgcg
    11161 ggcgatgtag aaggcttctt tgatgatgcg tccaaggtct tcccggtttc tgacggtcac
    11221 gccgtatttc gtgatgctgc gggtgatgcc gacgatatca acctcctgga atgcatcgtt
    11281 tccgatgagg tggcgggcta cctgtccggt gaagcagaca agcggaacgc tgtcgtagtt
    11341 tgccgttgca agaccggtga ccaggttggt tgcaccaggg ccgctggtaa cgaggcatac
    11401 gccgactttt cctgtcgttc tcgcataggc atccgcctca tgcaccagcg cctgctcgtg
    11461 gcgcggcaga acgattttga tttcatcctg tttgtaaagc tcatcgctga tatcaatggt
    11521 gcaagctccc ggataaccga agagatactc gacaccctcc tccttcagag cctttaccag
    11581 aagtttgttt cctgtaatct gtttcatatg aaaacctcct gttctaaatt tgctgcataa
    11641 aaaatccccg aacataaaac accttttctg tgccttacgt tcagggagaa tgtttccatc
    11701 cacggtacca cctgaattcc agaagaatac acttctgctc attccgtaca ggatttaact
    11761 tttttcatcc gatacggcat cccggtaacg tgggaactac gttggagcct acttggaaaa
    11821 cattgctgtc cgctttttct gttcagtcca ctgctcgagg gctacttccg caggcacgct
    11881 cacggagatt ctcaccagcc atctcctctc tttgctttgc gccgccaggt actcctccct
    11941 gtcactgcat tttaggtttt gttttttctt aaattactgt ctattatagt cagaaatgcc
    12001 gctcgttgtc aatcgtttcg cgcccttttc ttgacttttc gccctgcgtg tgctttactt
    12061 aatcaggttt gtctaattaa aataaggagt ttatacccat gatgattaca tacacacagg
    12121 aagaactctc ttccattgtc cacgcatttg ctttcccggg aacgattaca gactggacgc
    12181 cgcacgggaa cggccatatc aacgatacgt ttctggtgcg ctccaccgaa aacggcgtcc
    12241 tccgcaaatc gattctgcag cgcatgaacc gcaccgtttt ccacaatcca acggagctga
    12301 tgcagaatat tgcacaggta acatcatttc tgcggaaaaa aatcacagcc gcaggaggag
    12361 atccattccg cgagacgctg aacctgattc cgacgacaga cgatgcctgg tttttcgtcg
    12421 atgagcgcgg agaattctgg cgcgcatttc tctttatttc cgatgcgtcc tgctatgatg
    12481 ccgtgacaga tccaaagctg ttttacgaaa ccggtaatgc tttcggccgc ttccagcaga
    12541 tgctctccga ttttccgtcc gcctctcttt tcgagacgat cccggatttc cacaacacac
    12601 cgctccgcta ccaggcgctt ttacaggcgg cacaggcgga tccgaaagga cggctgaaaa
    12661 acgtttcgga tgagatggcg tttttcctcg accacgcaga cgattacggc gtagccgagc
    12721 gcatgaaggc ctcgggcgag ctgccgctcc gcgtaacgca caacgatacg aagctgaaca
    12781 acattatgat tgatgacaaa accggcgagg gcatctgcgt catcgaccta gacaccatta
    12841 tgccgggact gtcgatcttt gacttcggcg attccatccg cttcggcgcg aatacggccg
    12901 ctgaggacga acaggatgtc tcgaaggtgt ctctttccct tcctctcttt gagagctata
    12961 caaaaggctt tctcgcaggc tgcgcaggct ctttgacccg cgcggaagtc gaaatgctgc
    13021 cgatgggtgc aaaattaatg accctcgaat gcggcatccg ttttctcgcc gattacctga
    13081 tgggcgatac ctacttcaaa accgcttacc cggagcacaa cctcgtacgt gcgcggacac
    13141 agatcgcgct tgtagcggat atggaggaga aatggagcga gatggagaac atcgtcgcac
    13201 gggcttcgtt ttcccactaa gcacccgcat aaattcacag catcaaaaag acacagctgg
    13261 aagtatcttt ctgcctcctg ctgtgtcttt tttgtta
//
LOCUS       DAOMUF010000041        13124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1065_length_13124_cov_3.346239, whole genome shotgun sequence.
ACCESSION   DAOMUF010000041 DAOMUF010000000
VERSION     DAOMUF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13124
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1065_length_13124_cov_3.346239"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..853
                     /locus_tag="PLN77_03930"
     CDS             170..853
                     /locus_tag="PLN77_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43465.1"
                     /translation="MILELENICKEYMQGKIPVPVLKNINFSMEEGEYVAIMGPSGSG
                     KTTLMNIVGCLDQATSGRLILDGQDISKCSDNQMSDIRLQKIGFVFQNFQLLARQSAM
                     ENVCLPLTYAGVPKKERRERAAIALERVGLGDRMSFKPTQLSGGQKQRVAIARAIVNH
                     PKILLADEPTGALDSHSGEQVMELFQSLNDEGVSILMITHDAEIASMAQRTVEIRDGM
                     MKSKEVAET"
     gene            850..3237
                     /locus_tag="PLN77_03935"
     CDS             850..3237
                     /locus_tag="PLN77_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRM43466.1"
                     /translation="MKKGKAAKKAAIAVVTAVAVGGVSVGGYYGIRQAQKTTVKVYPV
                     SDFTSGYWGDSMNMEGQITSTASQNIYLSDSQTISQVLVKEGDVVNAGDTLMTYDTSM
                     IELDLETQQLQLAQTKLKIQQSEKELEKLKKTKPVSDTPLNPEDPDFPDFPDEPIDPD
                     FPDEPIPTPEPEPTYEDAVLYSSTNKLVFGSKPYKGEGTQEDPYTYLCAPGTVLTGGF
                     LNQMAGYADEKGTKKEEDSEGYWFRLEIHEENKAAEALETVWEQDGTKIAKAYETSYE
                     AVLSLKESESADAPEIKITTPLANTEVAEGGELKLKIGINEKKDTEEKPDEKADDKTD
                     DRTESPSEDDVTQPGEAGNGSGEADDSTENTETDVTYTYVWKHNGKTIDGDFKDTYTK
                     ENITKEDAGIYTVEVTAKNDKGTSMAVSTANVAVKEGTITPTPSVTPTPSVTPGPSVT
                     PEPTVSPEPSTTPIPTTEPVPTETPVPSETPVPTETPTPTEAPAEPEAEPQSEDAPEA
                     VQTTAKTVFYGEKASVQSLFTSSTGSSANNNTSSGDDTQMTYTKDELKKAISEKESQI
                     RNLKLDQKEEELKVKNTQKSLDSQTVKATISGVVKKVGNAENPSNDGSAFIQVSGNEG
                     LYVRGYLSETYLDQVKVGDELNVTSWSSGAFAAATVTEISPYPTTSYMSYSETPASFY
                     PFTAVIPEGGEGFENGDWIEIAITVGNDVENGNGLYVSKEFIREENGQKFVYIRDEND
                     KLKKQNVVTGKLLWGSYYEVKSGLSEEDYIAFPYGKTVVEGADTKESSSSDYYNS"
     gene            3249..4499
                     /locus_tag="PLN77_03940"
     CDS             3249..4499
                     /locus_tag="PLN77_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43467.1"
                     /translation="MLENVRLSFQGIWSHKMRSFLTMLGIIIGIASIISIVSTIKGTN
                     EQIKQNLIGAGNNNVTVSLKQGDGDYWMDNGLPSGVSKLSDDQKKRIREQDQVENASF
                     YTERTYVSGIAKGNTTLDSAKALGVDENYLSTCGYQIYQGRGFVESDFSKTHKVVLLD
                     DTAVLTLFPDESPVGKTIEIMGEPFTVIGVIKKADSFQPVINSRDDYYTYNQESYPLV
                     LMPDVCWPIVFQYDEPENAVVMAKTTEDMSTVGKTTADIMNESVTNTTDSLSYQAEDL
                     LEKAKSLQELGQSTNQQLIWIASISLLVGGIGVMNIMLVSVTERTSEIGLKKAIGARK
                     RRILFQFLTEAAVLTSIGGLLGVLGGIIMAQVISRVSQTPVAISIPAIIFSVVFSMVI
                     GIVFGLLPSVKAANLNPIDALRRE"
     gene            4775..5242
                     /locus_tag="PLN77_03945"
     CDS             4775..5242
                     /locus_tag="PLN77_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRM43468.1"
                     /translation="MNILFFITPKSETAYLYDDCSLRQAIEKIEYHKYTAVPVLNKKG
                     CYVGTLTEGDLLRIIKERYLLNYHEAEDIPLWQVPKRWNYESVNINSNIEDLIEMSMR
                     QNFVPVVDDEGIFIGIIRRRDIIQYCYQKSGIKEEEEARRSARKQNAEQMILQ"
     gene            5265..7031
                     /gene="argS"
                     /locus_tag="PLN77_03950"
     CDS             5265..7031
                     /gene="argS"
                     /locus_tag="PLN77_03950"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRM43469.1"
                     /translation="MKKLIDLIQEELVKAFTAAEMDPSYARVSVSNRPDLCEYQCNGA
                     MAAAKAYHKAPIQLAEAVVEKVTDHSVIGEIEAVKPGFINLKINPEFLADYLSKMQND
                     EDLSVEKAKNPKTIIVDYGGANVAKPLHVGHLRSAIIGESIKRMGRFMGHNMIGDVHL
                     GDWGLQMGLIITELQERHPELVYFDESFTGEYPEEAPFTISELEEIYPCASGKSKEDE
                     DYKKRALEATRELQQGRRGYRAIWKHIMNVSVADLKKNYGNLDVHFDLWMGESDAQEY
                     IPDMVDYLKDNGYAHYDQGALVVDVKEETDTKEIPPCMILKSDGAALYDTTDLATIIQ
                     RMKLYKPDEICYLADKRQELHFVQCFRCARKAKLVNDDTVLSFIGFGTMNGKDGKPFK
                     TREGGVMRLERLIGEINDEMYQKIVENRSVKDTDARGTAQIVGLSAIKYGDLSNQASK
                     DYVFDVERFTSFEGNTGPYILYTIVRTKSILGKYKEEGNELKKGALLAPKSDSEKALM
                     LSVSRFNGVVENAFEEKAPHKICAYIYELANEFNHFYHETKILSEQDEARKASYLALL
                     DLVREVLETCIDLLGFSAPERM"
     gene            7324..8781
                     /locus_tag="PLN77_03955"
     CDS             7324..8781
                     /locus_tag="PLN77_03955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRM43470.1"
                     /translation="MSVLGRGATSVVYLAEKTGSGKRYAVKVSICDREKLRREAFLLQ
                     KFSYPGIPCAVELLESGTKSFLVMEAVNGKSIGQRMRKGERFSSREILKTGIALSGIL
                     CYLHAQNPPVFYRDLKPDNVMITERGEIYLVDFGAAGTEETGVEVRYGTRGYAAPEQY
                     DGKCDARSDLYALGALLAAMAKHAKKRQKRGLWRVMEKCMQKKPEERYASAGEVKKAL
                     RRLERKQKGRKTLRIFAVFAFLTGGAAAVASRMPWGQFPAADEWEEQGDLWFCGNPVE
                     EGSLPNYQKAREAFLKADHLSQAGRVEQELVEYCLADDAAREKMQMEPLLAEFYDDTQ
                     QEPEKEKRCRRYLAVAGMYFSFSEELARENDTDAMRKGIEVLEKAAGENMEGQWEAVI
                     WQRLADACYLGGKSGDGEREQDWCQESFLYYERLLCSRQDGNRRKNLLRAAELALQFD
                     MQEKAEQYLNEAAEIPGMEEDIFYQQIRKRWEEAG"
     gene            8778..10256
                     /locus_tag="PLN77_03960"
     CDS             8778..10256
                     /locus_tag="PLN77_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43471.1"
                     /translation="MRKKTICAMMTGFLAVQGIEVHAETQQTTGEAASAVESVELEAE
                     IIENSDVTDADGKAGPEELLPDEKSEEEPPIEEKPEQEPEPPEEKTDPAETGKKETEG
                     EEKMLPPTVIPDPEKEPAERWEEEEEEISEDSESGKPEELPEAGDMGSGESESEEGCE
                     ETDTKESETEESDSERTDFGEMDTEKPDYEDTGSEKSGTENLMPEDPEPEKPDMEETK
                     QESPSTEKESENQTSETKAPEKEEPTVMPDAGKSNTEKQEQTGPGQSSPEKEELKTDE
                     QLPVAAAQQRLFAETAAATPEEVTQDPDDIPLQTEESSPPPAETPSESAGEEPSQIPP
                     QIIADFILSQIADEDFEAPIPLIIRPADTSVEIDPGYTPEILLEGAADTAEILSCMVN
                     GSEAEYEWKENKICLKAESLSEGYNRITVKVRGADGIVRTMKPWEVNVKPKTSTLVSQ
                     RTSQKPKGTRLWQSLRRLWNLIRAIAEIQNQTTRYLRALCRK"
     gene            10348..10977
                     /locus_tag="PLN77_03965"
                     /pseudo
     CDS             10348..10977
                     /locus_tag="PLN77_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            11527..11967
                     /locus_tag="PLN77_03970"
     CDS             11527..11967
                     /locus_tag="PLN77_03970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRM43472.1"
                     /translation="MASKIYEGQNPLLLILSVGVLSAAAEEIVFRGMIYRRAKDFWGT
                     GWGIAASCLLFGAYHGNVTQFVYAAVVSVLLILIYERCGTLLAPVLAHMAENIWGLYR
                     SQFVSWMTQKAPAGAWMFIFLEAVICAGTFWYLFLKDKQGKTQK"
     gene            12146..12880
                     /gene="rpsB"
                     /locus_tag="PLN77_03975"
     CDS             12146..12880
                     /gene="rpsB"
                     /locus_tag="PLN77_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRM43473.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAPYIYTERNGIYIIDLQ
                     KSVGKVDEAYQAVADIAAEGGSILFVGTKKQAQDAIKVEAERCGMYYVNERWLGGMLT
                     NFKTIKSRIARLKNIERMSEDGTFDVLPKKEVIQLKKEWDKLEKNLGGIKDMKTLPDA
                     IFVVDPKKERICVQEAHTLGIPLIGIADTNCDPEELDYVIPGNDDAIRAVKLIVSKMA
                     DAVVEANQGVAGEEEYVEEAEEAVEE"
     gene            12914..>13124
                     /locus_tag="PLN77_03980"
     CDS             12914..>13124
                     /locus_tag="PLN77_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HRM43474.1"
                     /translation="MAVTAKMVKELREMTGAGMMDCKKALNETNGDMDAAIEFLRKNG
                     EAKAVKKAGRIAAEGIVMADVKDDKT"
BASE COUNT         4060 a         2846 c         3635 g         2583 t
ORIGIN      
        1 acgatggtat gacgcaggat gaaaacggca tggtcgacaa taccatggac ggcgcagagt
       61 acagcggtga ggaagataca acgaacgtga tcggcggcgc cgacaatggt gaggcagaac
      121 cggcagaaac acaggaagcc ggatcagcag atgacgggga ggtgcagccg tgattctgga
      181 attagagaat atctgtaaag aatatatgca gggcaaaatc ccggttccgg ttctgaaaaa
      241 catcaacttc tccatggaag aaggcgagta cgttgcgatc atgggtcctt ccggttccgg
      301 aaaaacaaca ctgatgaata ttgtcggctg cctcgaccag gcgacgagcg gacggctgat
      361 cctcgacgga caggacatca gcaaatgcag tgacaatcag atgtcggaca tccgcctgca
      421 gaaaatcggc tttgtcttcc agaacttcca actccttgca agacagagcg caatggaaaa
      481 tgtatgcctg ccgttgacgt acgcgggcgt gccgaaaaaa gagagaagag agcgggcagc
      541 gattgccctc gagcgggtgg ggctcggcga ccgtatgagt ttcaaaccga cccagctctc
      601 cggcggacag aaacagcgtg tggcgattgc aagagcgatt gtcaaccatc cgaaaattct
      661 gctggctgat gagccgaccg gagcgctcga ctcccattcc ggcgagcagg tcatggagct
      721 gttccagagc ctgaacgacg agggcgtcag catcttgatg attacacatg acgcggagat
      781 cgcatcgatg gcacagcgca ccgtggagat ccgcgacgga atgatgaaat cgaaggaggt
      841 ggccgaaaca tgaaaaaagg aaaagcagca aagaaagccg cgattgcagt tgtgaccgca
      901 gttgccgtgg gcggtgtcag tgtcggcggt tattacggca tccgccaggc gcagaagacg
      961 accgtaaaag tgtatccggt cagtgacttt acgagtggtt actggggaga cagcatgaac
     1021 atggaaggcc agatcacctc gacggcttcc cagaacatct atctttccga cagccagacc
     1081 atttcccagg ttctcgtcaa agagggcgat gtggtaaatg ccggagacac gctgatgaca
     1141 tacgatacca gcatgatcga gctggatctg gaaacgcagc agttacagct tgcacagaca
     1201 aagctgaaaa tccagcagag tgaaaaagaa ctggaaaagc tgaaaaagac aaaaccggta
     1261 tcggatacgc cgctgaaccc ggaagatccc gactttccgg attttccaga tgaacccatc
     1321 gatccggatt tcccggacga accgattccg accccagaac cggagccgac gtatgaggat
     1381 gctgttctgt acagcagtac gaataagctt gtatttggtt ccaaaccata caaaggagaa
     1441 ggaacgcagg aagacccata cacctatctc tgcgcaccgg gaacagtcct gacaggcgga
     1501 ttcctgaatc agatggcagg atacgcggat gaaaagggaa cgaaaaaaga agaggacagc
     1561 gaaggatact ggttccgcct ggagattcat gaagaaaaca aagccgcaga ggcgctggaa
     1621 accgtgtggg agcaggatgg aacgaagatt gcgaaagcat acgagacttc gtatgaagca
     1681 gtcctgagtc tgaaagaatc cgaatcggcg gatgcaccgg aaataaaaat caccacgccg
     1741 cttgccaaca cagaggtggc agagggcggc gagctgaagc tgaaaatcgg aatcaatgag
     1801 aagaaagata ccgaagagaa gccggatgag aaagccgacg ataaaacgga tgatagaacc
     1861 gagagtccgt ccgaggacga tgtgacacag ccgggtgaag ccggaaatgg aagcggtgag
     1921 gcagacgatt ccacggaaaa caccgaaacg gatgtcactt acacatacgt ctggaaacac
     1981 aacggaaaga caatcgacgg agattttaag gatacttaca caaaagaaaa catcacaaaa
     2041 gaggatgccg gaatctatac agtagaagtg acagcgaaaa atgacaaggg tacttccatg
     2101 gcagtcagca ctgcaaatgt ggcggtaaaa gaaggaacga ttaccccgac accaagtgta
     2161 actccgaccc cgagcgttac gccgggaccg agtgtaactc cagagccgac cgtcagcccg
     2221 gaaccaagta caacaccgat tccgaccaca gagccggttc caacagaaac tccggtgccg
     2281 agcgagaccc cagttccgac ggaaacgccg acgccgaccg aagccccggc agaaccggaa
     2341 gcggaaccgc agagcgaaga tgcgccggaa gcagtgcaga cgacagcaaa aaccgtattc
     2401 tacggtgaaa aagcatcggt acagtccctc tttacatcat ccacaggaag cagcgcaaat
     2461 aacaatactt cttccggtga tgatacccag atgacctaca caaaagatga gttgaaaaaa
     2521 gccatcagcg aaaaagaaag ccagatccgt aatctgaagc tggatcagaa ggaagaggaa
     2581 ctcaaagtca aaaatacgca gaaatccctg gattcccaga ccgtaaaagc gacgatcagc
     2641 ggtgtggtaa agaaggttgg aaatgcagaa aacccgtcca acgacggaag cgcctttatc
     2701 caggtcagtg gaaacgaagg tctgtacgtg cgcggctact taagcgagac gtatctcgat
     2761 caggtaaaag tcggcgatga actcaacgtg acttcttggt cttcgggtgc ttttgcagcg
     2821 gcaaccgtaa ccgagatttc tccgtacccg acaacaagct acatgtcgta cagcgagaca
     2881 ccagcctcct tctatccgtt cacagccgtt atcccggaag gcggagaagg ttttgaaaac
     2941 ggtgactgga tcgagattgc aatcactgtc ggaaacgatg tagaaaacgg caacggcctg
     3001 tacgtcagca aagaattcat ccgtgaggag aacggacaga aattcgttta tatccgggat
     3061 gaaaatgata aattaaagaa acagaatgtg gtaaccggaa aacttctgtg gggcagttat
     3121 tacgaagtga agagcggtct ttccgaagag gattacattg catttccgta cggcaaaacc
     3181 gtggtggaag gcgcagacac gaaggaatcc agttcatcgg attattacaa cagttaagga
     3241 ggcgcaccat gttagaaaat gtaagacttt cctttcaggg aatctggtca cacaaaatgc
     3301 gttccttcct gacgatgctg ggtattatca tcggaattgc gtcgatcatc tcaattgtct
     3361 ccacgatcaa gggaacgaat gagcagatca aacagaacct gatcggagcc ggaaacaaca
     3421 acgtgaccgt cagcttaaag cagggcgacg gtgattactg gatggacaac ggactgccaa
     3481 gcggcgtgtc gaaactgtcc gatgatcaga aaaagcggat ccgcgagcag gatcaggtgg
     3541 aaaatgcatc gttttacacc gaaagaacgt atgtgagcgg catcgcaaaa ggaaacacaa
     3601 cactggacag tgcgaaggcg cttggcgtgg atgaaaatta tctgtcgacg tgcggatatc
     3661 agatttatca gggacgcggc tttgtggaga gcgatttctc gaagacccac aaagttgtcc
     3721 tcctcgacga taccgcagtg ctgacactct tcccggatga gtcgccggtc ggcaaaacga
     3781 tcgagatcat gggagagccg ttcacggtga tcggcgtgat caaaaaagcc gattcgttcc
     3841 agccggtcat caattccaga gatgactact atacgtacaa tcaggaatct tatccgctgg
     3901 tgctgatgcc ggatgtctgc tggccgatcg tattccagta tgatgaaccg gaaaatgccg
     3961 ttgtgatggc aaaaacgacc gaggatatga gcacggtggg aaagacaaca gcggatatca
     4021 tgaacgaatc cgtgaccaat acaactgatt ctctttccta tcaggcagag gatctgctgg
     4081 agaaagcaaa gagtctgcag gagttaggcc agagcacgaa ccagcagctg atctggatcg
     4141 cgagcatttc gcttctggtc ggcggtatcg gtgttatgaa tattatgctg gtatctgtaa
     4201 cggaacgtac aagcgagatc ggcctgaaaa aggcgatcgg agcaagaaaa cgcagaattc
     4261 tgttccagtt cctgaccgag gcggcggtgc tcaccagcat cggcggactg ctcggcgtgc
     4321 tgggcggcat tatcatggca caggtgattt cgagggtatc gcagacgcct gtggcgatca
     4381 gtattccggc gatcattttc tccgtggtct tctccatggt gatcggtatc gtattcggac
     4441 tgctgccgtc ggtaaaagca gcgaacctga atccgatcga tgcgctgcga agagaataga
     4501 acaaaaaaga gttgaaaaat ccgtgcgggt acagcgtagg tgctggatcc gtgcggattt
     4561 tttgtgcggc gtgtgcggat gaaacttaag atctttactt tttgcggaaa gtcggttata
     4621 ctggaaaata agcgcaattt gcggcgcagc aaagaggcat tcgagtttcg gattcggtat
     4681 gtttatgaaa atataagaaa gattcaaaga catttcacat tcgtttatgg tataatcaaa
     4741 caagagtatg accaacgaaa aggagtatac gcgaatgaat attttgtttt ttatcacacc
     4801 gaaaagtgaa acggcttatc tgtatgacga ctgctcactt cggcaggcca tcgagaagat
     4861 cgaatatcat aaatacacgg cggttccggt actgaacaaa aagggctgct acgtgggaac
     4921 actgacggaa ggagatctgc tccgcatcat aaaagaacgg tatctgctga attaccatga
     4981 agcagaggat atcccgctct ggcaggttcc gaaacggtgg aattatgagt ccgtcaacat
     5041 caacagcaac atcgaggacc tgatcgagat gtccatgcgg cagaatttcg ttccagttgt
     5101 ggatgacgag ggaatcttca tcggcatcat ccgaagaaga gatatcatcc agtactgtta
     5161 tcagaaaagc ggcattaaag aagaggaaga agcccggcgc agcgcgcgga aacaaaacgc
     5221 agaacagatg attctgcagt aaagaagaaa atgagggaaa cattatgaaa aaactgatcg
     5281 atcttatcca ggaagagctg gtaaaagcct ttaccgctgc ggagatggac ccgtcctatg
     5341 caagggtttc cgtttccaac cgcccggatc tgtgcgaata ccagtgcaac ggcgcaatgg
     5401 cagctgcaaa agcataccac aaagcaccga tccagctggc tgaagcagtt gtagaaaaag
     5461 tgaccgatca cagcgtgatc ggtgaaattg aagccgtaaa accgggattc atcaacctga
     5521 aaatcaaccc ggaatttctg gcagattatc tttccaaaat gcagaacgat gaagatcttt
     5581 ctgtagagaa agcaaaaaat ccgaaaacca tcatcgtaga ttacggcgga gcaaacgtgg
     5641 caaaaccgct tcacgtcggc catctccgtt ccgcgatcat cggggaaagc attaagcgta
     5701 tgggacgctt catgggccac aacatgatcg gtgacgttca tcttggagac tggggtctgc
     5761 agatgggact gatcatcacc gagctgcagg agcgccatcc ggagcttgtt tattttgatg
     5821 agtcctttac gggagagtac ccggaggaag caccgtttac gatcagcgag ctcgaggaga
     5881 tttatccttg cgcaagcggc aaatcgaaag aggacgagga ctataagaaa cgtgcactcg
     5941 aggcaacgag agagctgcag cagggcagaa gaggatatcg tgcgatctgg aaacacatta
     6001 tgaacgtttc tgttgccgat ctgaaaaaga actatggaaa cctcgacgtt catttcgatc
     6061 tgtggatggg tgagtccgat gcacaggagt acattccgga catggtagat tacctgaaag
     6121 acaatggtta tgcacattat gaccagggcg cactcgttgt tgatgtcaaa gaagagacag
     6181 acacaaaaga gatcccgccg tgcatgatct taaaatccga tggagcagct ctgtatgata
     6241 cgacagacct ggcaaccatc atccagcgta tgaaactgta caagccggac gagatctgct
     6301 atctggcaga caaacgtcag gagcttcact tcgtacagtg tttccgctgt gcaagaaaag
     6361 caaaactggt gaatgatgat accgttcttt cctttatcgg ctttggtacc atgaacggaa
     6421 aagacggaaa accgttcaag acccgtgagg gcggcgttat gcgtctggag cgtctgatcg
     6481 gcgagatcaa cgatgagatg tatcagaaga tcgtggagaa ccgcagcgta aaagatacgg
     6541 atgcaagagg aacggcacag attgtcggac tgtctgctat taaatacggt gacctgtcca
     6601 accaggcatc gaaagattat gtattcgatg ttgagagatt tacttccttc gagggaaaca
     6661 ccggtccgta catcctgtac acgatcgttc gtacaaaatc catcctcgga aaatataaag
     6721 aagagggaaa tgaactgaag aaaggcgcac ttctggcacc gaagagcgac agcgagaaag
     6781 ctctgatgct ttccgtatcc cgtttcaacg gcgttgtgga gaacgcattc gaggagaaag
     6841 caccgcataa gatctgtgct tatatctacg agcttgcaaa cgagttcaac cacttctacc
     6901 acgagaccaa gatcttaagt gagcaggatg aggcaagaaa agcatcttac ctggcactgc
     6961 tggatcttgt ccgcgaggta cttgagacct gcatcgacct gctgggattc tcggcaccgg
     7021 aacggatgta agattcgtca agagggaaaa aatagataaa aaacaggcga tttttttggc
     7081 agccgtcggc ttctgtagtt cttgcagaaa cggcggctgt tgtgttatac ttacatcacg
     7141 atttttcatc cgcttggaca ttcgtcctga aaaatcgatt ctatattact ctatcgaaga
     7201 gccattcggc tcaatttata tccatgtaca aaaacagaat atcggactga caaacacttt
     7261 gacgtaaaaa gaggaggcgg gatcattgga aaagtagaat ttccgggagg gaagtatcgc
     7321 ctgctgtcag tgctcggaag aggggcaacg tctgtcgttt atcttgcaga gaaaactgga
     7381 tccggaaagc ggtatgcagt caaggtaagt atatgtgaca gggaaaaact gcgccgggaa
     7441 gcgtttcttt tacagaagtt ttcatatccc gggattccct gtgcggtgga acttctggaa
     7501 agtgggacaa aaagctttct ggtgatggaa gctgtaaacg gaaaatctat cggacagcgt
     7561 atgcgtaagg gagaacggtt ttccagccgg gaaattctga aaacgggaat tgccctgagc
     7621 gggattctct gttatctcca tgcacagaac ccgccggttt tctaccggga tctgaagccg
     7681 gacaacgtga tgataacaga gcgtggtgaa atataccttg tggatttcgg tgcagccgga
     7741 acagaagaaa cgggtgtgga agtccgttat ggaacccgcg gttatgcagc gccggagcag
     7801 tatgacggga agtgtgacgc ccgttccgat ctttatgcac tcggcgcact gcttgcggca
     7861 atggcgaagc atgcgaaaaa gcggcagaaa agaggactct ggcgggtgat ggaaaagtgt
     7921 atgcagaaaa agccggagga gcggtatgcg tctgccggag aagtcaaaaa ggcactgcgg
     7981 cggctggaaa gaaagcaaaa aggcaggaag actctgcgga tctttgcagt ttttgctttt
     8041 ctgacaggag gagcggctgc agttgccagc aggatgccat gggggcagtt tccggcggca
     8101 gacgaatggg aggagcaggg agatctctgg ttctgcggaa atccggtgga agaaggcagt
     8161 ctgccgaact accagaaagc gcgggaagcg ttcttaaagg cagaccatct cagtcaggcg
     8221 gggcgtgtgg agcaggaact tgtggaatac tgtctggcgg atgatgcggc aagggaaaaa
     8281 atgcagatgg agccattgct ggctgaattt tatgatgaca cgcagcagga gccggaaaaa
     8341 gaaaaacgct gccgcagata tcttgctgtt gcaggaatgt atttcagttt ttccgaagaa
     8401 ctggcgcgtg aaaatgacac agatgccatg cggaaaggaa tcgaagtgct ggaaaaggcc
     8461 gcaggcgaga atatggaggg gcagtgggag gcggttatct ggcagagact ggcggatgcg
     8521 tgctatcttg gcggaaaatc cggggatggg gaaagagaac aggactggtg ccaggagagc
     8581 tttctctatt acgaacggct tctgtgcagc agacaggatg gaaaccggcg gaaaaatctg
     8641 ctcagggccg cagagctggc acttcagttc gacatgcagg aaaaagcaga acagtacctc
     8701 aacgaggcag cagaaattcc gggaatggag gaggacattt tttatcagca gatcagaaaa
     8761 agatgggagg aagcaggatg agaaaaaaga ccatatgcgc aatgatgaca ggattcctgg
     8821 ccgtccaggg aatcgaagtc cacgcggaga cacagcagac gacaggcgag gccgcaagtg
     8881 cggtggaaag cgtggaatta gaagctgaaa tcatcgagaa cagtgatgtg acagatgcgg
     8941 acgggaaagc cggaccggag gaactgctgc cggacgaaaa gtcagaagaa gaaccgccta
     9001 tagaagagaa accagagcag gaaccagagc cgccggaaga gaaaacagat ccggcagaga
     9061 ccggaaagaa agagacagaa ggagaagaaa aaatgcttcc cccgacggta attccggatc
     9121 cggagaaaga accagcggaa aggtgggaag aagaggagga agaaatttcg gaagattcag
     9181 aatcggggaa gccagaagaa ttgccggagg caggagatat gggttccggg gaatcggaat
     9241 cagaggaagg atgtgaagaa acagatacaa aagaatcaga gacagaagag tcagattcgg
     9301 agagaacgga ttttggagaa atggacacag aaaaaccaga ttatgaagat acaggttcag
     9361 aaaaatccgg aacggaaaat ttgatgccag aagatccgga accggaaaag cctgatatgg
     9421 aagaaacgaa gcaggaatct ccgtcaaccg aaaaagagtc agaaaatcaa acctcggaaa
     9481 caaaagcacc agaaaaagag gagcctaccg taatgccaga tgccggaaag tcaaacacag
     9541 aaaagcagga acaaacgggg cccgggcaat cgtctccgga gaaagaagaa ctcaaaacag
     9601 acgaacagct gcccgttgct gcagcccaac agcggctgtt cgcggagacc gccgcagcca
     9661 cgcccgagga ggtgacccaa gatccagatg atattccact acaaacagaa gaaagcagtc
     9721 ctccgcctgc cgaaactcca tctgagagtg ccggggagga gccatctcaa ataccgccgc
     9781 agatcatcgc cgatttcata ttatcacaga tagcagacga agatttcgaa gcgccgatcc
     9841 ccctgattat ccgcccggcc gacaccagcg tcgaaataga tcccggttat accccggaaa
     9901 ttctgctcga gggagccgcc gacacggctg aaattctttc ctgcatggta aatggctccg
     9961 aagccgaata tgaatggaaa gaaaacaaaa tctgtctgaa agccgagagc ctttccgaag
    10021 gctacaaccg catcactgta aaagtgcgtg gcgcagacgg cattgttcga accatgaaac
    10081 cgtgggaagt caacgtgaaa ccaaaaactt ccacacttgt cagccaaaga accagccaga
    10141 aaccaaaagg aacccgcctc tggcagtccc tgaggcgcct ctggaacctg attcgtgcca
    10201 ttgccgaaat tcagaatcag accacccgct atctgcgtgc actctgcaga aaatagcctg
    10261 atccggtttt gtttgtattt ctaatttcga tatgctagaa taaaagacat gtcatacgat
    10321 aaaaaaagta aaagaggaaa taagagtatg tcagatatga tattatttga tctggacgga
    10381 accctgaccg attccggtcc tggaattaca agatgcgtac agtatgcgct ggcatccttt
    10441 ggcatcgaag agccggatct ggaaaaactg aactgttacg tgggaccgcc gcttctggaa
    10501 tctttcatga attttgcagg ccttggctgc gaagaagcac agcaggccat cacaaaatac
    10561 cgggagcggt acgaggcaga gggcattttt gaaaatgaag tatacgaggg aatcccggag
    10621 gttctggcgt atctgaaaga gaagggaaaa attctggcgg tagcatcgtc caaaccggaa
    10681 aaatacgtgg agcagatcct ggagcatttc gaaatcagaa agtattttac cgtagtaacc
    10741 ggttcagaaa tgaacgaaac cagaaccgac aaaggagaag tcatcgcaga gacgctgcgc
    10801 cgactcggag cagaagacag ccgttccgac gtggtgatgg ttggtgacag aagctatgac
    10861 gtaatcgggg caagagaaaa cggactgctc tgtgtcggcg tatcgtatgg gtacggtgga
    10921 agagaagaac tcgaggcagc aggtgccgca aaggtatgtg atacaccgga agaactgaaa
    10981 ctgctggcgg aggatccgaa agaagagaag aaaagacgcg accgcatcaa aaaaatgaag
    11041 ccggagcgta tcccgtggct gacaagagga gaaagtggaa caggaatctt tgcgccgaaa
    11101 gagaaaaaag cagagaatat aaaagaaaaa aggacagcga aaacagagcc aaaaacggag
    11161 gaaatcgtaa ttcctatcca gcggaagcta tggcgtcttt taaaaccgct tctgacgtac
    11221 gaactgtttc tgatcggagc aaccgtactt ctggcgctga tcctgatgac gtttacgacc
    11281 ggcggttatc tggaagagcg tatgcacgcg acatccgttc ttgcatcagc aatcggcagt
    11341 ctggcggcga tcccggttct ctggaaacag tacaaaaaag atgaaggcat gttcccgaaa
    11401 ccggaaaaac ggtggagtgc ggcagaggca gccgcctgta ttctgttggt tatgaccttc
    11461 ggccatctcc tgaacactgt catgaacgtg accggcttta ccagaatctt ttccggctat
    11521 caggacatgg catcaaagat ttatgaagga cagaacccgc tgcttctgat cctgtccgtc
    11581 ggtgtgctga gcgcggccgc agaggaaatt gttttccgcg gcatgattta ccgcagggca
    11641 aaagatttct ggggaaccgg ctggggcatc gcagcatcat gtctgctgtt cggtgcatat
    11701 cacggcaatg tgacgcagtt tgtctatgca gccgtggtaa gtgtgcttct gatccttatt
    11761 tatgagcggt gcggaacact ccttgccccg gttcttgcgc acatggcaga aaatatctgg
    11821 ggactttacc ggagccagtt cgtatcctgg atgacccaaa aagcgccggc aggtgcatgg
    11881 atgttcatct ttttggaagc ggtaatctgc gccggaacgt tctggtatct gtttttaaag
    11941 gataaacagg gaaaaacaca aaaataacag aaaaaaaccg cagaatccgc gaaaaaaatg
    12001 cttgtcatga aaaaaactct gtgatacaat cttactgttg cacaataagc acccatgcgg
    12061 taccaaggat ggtgcctgtt ttttgaaaaa caggttccaa ggtgaaaaaa catggggaag
    12121 aataaaacca taaggagata aaacaatgag cgtaatttca atgaaacagt tactggaagc
    12181 cggtgttcat ttcggacatc agacaagaag atggaaccct aagatggctc cttatatcta
    12241 cacagagaga aatggtatct acatcatcga ccttcagaaa tctgtaggta aagtagatga
    12301 agcttaccag gcagtagctg atatcgctgc agaaggcgga tcaatccttt ttgtaggtac
    12361 taagaaacag gctcaggatg ctatcaaagt agaagctgaa cgttgcggaa tgtactatgt
    12421 aaatgaaaga tggcttggag gaatgcttac aaacttcaag acaatcaaga gcagaatcgc
    12481 tcgtctgaaa aacatcgaga gaatgtcaga agacggaaca ttcgatgtac ttccgaaaaa
    12541 agaagttatc cagttaaaga aagaatggga taaacttgag aagaaccttg gcggaatcaa
    12601 agacatgaag acacttccag acgctatttt cgtagtagat cctaaaaagg aaagaatttg
    12661 tgttcaggaa gcacacacac ttggaattcc acttatcggt atcgctgata ctaactgtga
    12721 tccagaagaa cttgattatg taattcctgg aaacgacgat gctatccgcg ctgtaaaact
    12781 gatcgtttct aagatggctg acgctgtagt agaagctaac cagggtgttg caggcgaaga
    12841 agagtatgtt gaagaagctg aagaagcagt agaagaataa gattcttaaa aaacataatt
    12901 ggaggataag atcatggcag ttacagctaa gatggtaaaa gaattaagag agatgactgg
    12961 tgcaggaatg atggactgca agaaagctct taatgagaca aatggtgata tggatgcagc
    13021 tatcgagttc ttaagaaaga acggagaagc taaggctgtt aaaaaagccg gacgtatcgc
    13081 agcagaaggt attgttatgg ctgatgttaa ggatgacaag acag
//
LOCUS       DAOMUF010000042        13015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1079_length_13015_cov_3.904090, whole genome shotgun sequence.
ACCESSION   DAOMUF010000042 DAOMUF010000000
VERSION     DAOMUF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1079_length_13015_cov_3.904090"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..757)
                     /gene="ispD"
                     /locus_tag="PLN77_03985"
     CDS             complement(29..757)
                     /gene="ispD"
                     /locus_tag="PLN77_03985"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRM43475.1"
                     /translation="MNEKKEKYTAVVLAAGKGTRMGGSVAKQFLEIGGKPVVVYALEV
                     FEHSPEIDQIILVTGADKIEYCQKEIIEKYGIKKTVSVCAGGRERYESVWKALQTLKS
                     LESEQEQKTDYVLIHDGARPFLNQEMLERLCACVKEEKACVLGMPVKDTIKLTDENGK
                     IRESPRRDLVWQAQTPQAFAAELLFPAFEKLMQHSTEGITDDAMVVEQEMGIASVMVK
                     GGYENIKITTPEDLLVAESFLAEK"
     gene            complement(837..1862)
                     /gene="tsaD"
                     /locus_tag="PLN77_03990"
     CDS             complement(837..1862)
                     /gene="tsaD"
                     /locus_tag="PLN77_03990"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRM43476.1"
                     /translation="MSEKDTLILAIESSCDETAASVVKNGRCVLSNIISSQIAIHTLY
                     GGVVPEIASRKHIEKINQVVEAALKEADVTLDDIDAIGVTYGPGLVGALLVGVAEAKA
                     IAYAKKKPLVGVHHIEGHVSANYIEHPDLEPPFLCEIISGGHTHLVIVKDYGSFEILG
                     RTRDDAAGEAFDKVARAIGLGYPGGPKIDKLAKEGNPHAIDFPRAHMEDAPYDFSFSG
                     VKSAVLNHLNKCRMTGEPIVEADIAASFQQAVVDVLVDNAIRAAKDYHMDRLAIAGGV
                     ASNGALRAAMEAACEKEGIRFYRPSPIFCTDNAAMIGVAAYYEYQKGTRHGWDLNAVP
                     NLKLGER"
     gene            complement(1855..2778)
                     /locus_tag="PLN77_03995"
     CDS             complement(1855..2778)
                     /locus_tag="PLN77_03995"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRM43477.1"
                     /translation="MLDVCLLGTGGMMPLPGRRLTALMTRYNGSSLLIDCGEGTQVAI
                     KEKGWTFKPIDAICFTHYHADHISGLPGLLLTMGNTDRREPLTMIGPKGLERVVNSLR
                     VICPELPFPIVFHEVTAPEEVIEMNGYKITAFRVQHNITCYGYSLEISRKGKFDVERA
                     KAQEIPIKCWNPLQKGETVTLDGKTYTPDMVLGPARKGLKVTYCTDTRPIPSISEHAK
                     GSDLFICEGMYGEPEKQAKAKEFKHMTFYEAAKLAKDAEAAEMWLTHFSPSLVGPQRY
                     MDEVQKIFPAAELGKDGRSVELLFEEETKNE"
     gene            complement(2916..3698)
                     /gene="trpA"
                     /locus_tag="PLN77_04000"
     CDS             complement(2916..3698)
                     /gene="trpA"
                     /locus_tag="PLN77_04000"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRM43478.1"
                     /translation="MSKVTEAFSNGKAFIPFLTAGDPNIEKTENYILELEKAGADLIE
                     IGIPFSDPIAEGVVIQEADLRSLKAGTTTDKIFDMVASVRKKTDIPLVFMTYLNPVFH
                     YGYEPFFTKCEEVGINGIIIPDMPYEEKGELNDIARSHQVDVISLIAPTSEQRIQKIA
                     ADAEGFIYLVSSLGVTGVRSEIKTDLDSMIAAIRKVTDIPVAVGFGINTTDQAANIAK
                     IADGVIVGSAIVKMIAKYGTHAEQHIYTYVRMMKEAVMTANK"
     gene            complement(3691..4875)
                     /gene="trpB"
                     /locus_tag="PLN77_04005"
     CDS             complement(3691..4875)
                     /gene="trpB"
                     /locus_tag="PLN77_04005"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRM43479.1"
                     /translation="MSDGRFGIHGGQYIPETLMNDIINLEKQYNYYKNDPEFCQELDD
                     LLKNYAGRPSLLYYAEKMTKDLGGAKIYLKREDLNHTGAHKINNVLGQCLLAKRMGKT
                     RVIAETGAGQHGVATATAAALMGLECEIFMGKEDTDRQALNVYRMELLGAKVNAVTTG
                     TMTLKDAVNEAMREWVSRVDDTHYVLGSVMGPHPFPTIVRDFQAVISKEAREQILEKE
                     GKLPKAVMACVGGGSNAMGMFYNFINDKNVRLIGCEAAGRGVNTAETAATIATGTLGV
                     FHGMKSYFCQDKYGQIAPVYSISAGLDYPGIGPEHAYLHDIGRAEYVPVTDDEAVEAF
                     EYLARTEGIICAIESAHAVAHAMKIAKEFDPEDSIIICLSGRGDKDVAAIARYRGRDL
                     HE"
     gene            complement(4868..5476)
                     /locus_tag="PLN77_04010"
     CDS             complement(4868..5476)
                     /locus_tag="PLN77_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRM43480.1"
                     /translation="MEEQTRIKICGLTRLEDIDAVNELKPEYVGFVFAKSRRQITKEH
                     AVTLRRYLDPEIQAVGVFVNELPAIVAGYLEEGVIDLAQLHGNESEEYIHSLRFRTGG
                     ELIKAFSIKTREDVEKAKKSSADYILLDHGKGGTGESFDWSLIRNMDRPYFLAGGLNA
                     ENVEQAILQTHPFAVDISSGVETDGVKDPKKITECIRRIRHV"
     gene            complement(5530..6312)
                     /gene="trpC"
                     /locus_tag="PLN77_04015"
     CDS             complement(5530..6312)
                     /gene="trpC"
                     /locus_tag="PLN77_04015"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRM43481.1"
                     /translation="MILETIAEATRKRVAAEKEKCSLAEMKEKATAMNPDTGFPFEQA
                     LKKPGMSFICEVKKASPSKGIIAEDFPYLEIAKEYEAAGASAISCLTEPEYFKGDKRY
                     LKKIAETVSIPVLRKDFTVDPYMIYEAKVLGATAVLLICALLPEETLKEYLETAHSLG
                     LSALVEAHDEAEVAQAMRAGARIIGVNNRNLKDFTVDIHNSERLRELVPENIVFVSES
                     GIKTPEDVGRLYQNKTDAVLIGETLMRSSNKKAELEKLSSMC"
     gene            complement(6317..7336)
                     /gene="trpD"
                     /locus_tag="PLN77_04020"
     CDS             complement(6317..7336)
                     /gene="trpD"
                     /locus_tag="PLN77_04020"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRM43482.1"
                     /translation="MIREAIVKLVNKENLTYEMAEGAMDEIMGGKADPIQISAFLTAM
                     TMKGETIEEITACANGMRKHGVHMEHDKDVLEIVGTGGDKSNSFNISTTASLVISAGG
                     VAVAKHGNRAASSKSGAADCLEALGVKIDLEPEKNKEVLEKLGICFLFAQKYHMSMKY
                     VAPVRKMLGIRTIFNILGPLTNPAAATMQVMGVYEEALVRPMAEVLSNLGVKRGMVVY
                     GQDCLDEISLSAPTSVCEIKDGTFEEYVITPEEFGMTRCTKEELVGGTPQENAEITKE
                     ILAGKKGPVRDAVVLNAAAALHVAKEIPMQDAVKLAEELIDSGKAQKKLEEFVSLTNK
                     LAEKE"
     gene            complement(7371..7943)
                     /locus_tag="PLN77_04025"
     CDS             complement(7371..7943)
                     /locus_tag="PLN77_04025"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRM43483.1"
                     /translation="MILLVDNYDSFSYNLYQLAGSINPDMKVIRNDEMTIDEICALAP
                     ASIILSPGPGRPEQAGRCMEIVEKLGGEIPILGVCLGHQAICAAYGATVTYAKQLMHG
                     KQSETTLDLTSELFQGLPETVPVARYHSLAADEATLPETLKVTARTADGEVMAVEDSE
                     KKIYGVQFHPESILTPQGRQMVENFLKITR"
     gene            complement(7940..9481)
                     /gene="trpE"
                     /locus_tag="PLN77_04030"
     CDS             complement(7940..9481)
                     /gene="trpE"
                     /locus_tag="PLN77_04030"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRM43484.1"
                     /translation="MKQMTLEEISKAAVSGAFRKIPVSREIYSDIRTPVETLKVLQGV
                     SSHCYMLESVEDKKQWGRYTFLGYDPSLELTCVNGNLTITADAAEMKKVEDIPESHKE
                     QLPTGQIRLTAKTAHPGAVIKTLIEKNKSPKIATLPAFTGGLVGYFSYDYIKYSEPTL
                     KLDAEDQEHFKDVDLMLFDKVIAYDNYRQKIVLMINIETENLEENYEKAVQELEKMEE
                     LIRFGKPAETKAGHLKSEFRPLFDEKAYCEKVEQVKHYIHEGDLFQLVLSNRLEADFE
                     GSLLDTYRVLRTTNPSPYMFYFSSDDVEIAGASPETLVKVEDGEVRTFPLAGTRSRGK
                     SYEEDQRLEKELFADEKERAEHNMLVDLGRNDLGKICDFGTVEVEKYMSIERYSHVMH
                     IGSTVKGQLRKDKTAVDAIDSVLPAGTLSGAPKLRACQIINELENNKRGIYGGAIGYL
                     DFTGNMDTCIAIRLAFKKNGKVFVRSGAGIVADSVPEKEHQECINKAKAVMVALAEAE
                     GGSNL"
     gene            complement(9918..10694)
                     /locus_tag="PLN77_04035"
     CDS             complement(9918..10694)
                     /locus_tag="PLN77_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43485.1"
                     /translation="MSGYILCQVKRAKNPYYISNISTNIYSIEELCYYLYHNIYLLDE
                     TIINEQLLVWMKEELHLRRLYQRLYVLLEEKKNIGEFILPIFKEINYLSHDQFREMNE
                     RLKRFEEQPEIVRKKIKGDYLVDHEKYINAIQVYQETLKDTEENETNMGSQFTGSIYN
                     NMGCAYASLFQMNEALTCFQKANEELHTKASLKSWLFAVYMSKGQDAYEQMCTERKVD
                     AETKREMDRQITEAMQVELPRDLDEALAAWTREYHKNTGL"
     gene            complement(10698..11945)
                     /locus_tag="PLN77_04040"
     CDS             complement(10698..11945)
                     /locus_tag="PLN77_04040"
                     /inference="COORDINATES: protein motif:HMM:NF039205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HRM43486.1"
                     /translation="MEQKNLILGFDFGEKYSQFCCYDRGTHTAVSIPVKEGEEAVEFP
                     TAIAKKRNEETWKTGPDAEKSAHAENGIWLDNLYEICMGSRICQIENRDYTPGEVLGT
                     FLREALKMIGIERPDQQIQAMMITTGHLTRPFVENVREAYKIIGLPRGRAYLQEHDES
                     FYCHVLNQKPELWSRKVGLFFLKDEEASFSELSISRKTKPATVTVKRGPKAALSIEPM
                     ERDRDFCHLMGEAMGNEIYSSVFLVSEEFDLAWADNSLRQLKKNQRRIFGGTNLFAQG
                     ACFSAREKVEERRLKGYLFLGNDLVRYNIGMEMTINGSPAYYALIAAGVNWYEAEKEC
                     ELILDGTEELEFVVSSMESGKRNRYTMKLDGLPKRPPKTTRIRLRLEYDSPVTCQITA
                     EDLGFGDMFPASHKIWHETMGEV"
     gene            complement(11927..>13015)
                     /locus_tag="PLN77_04045"
     CDS             complement(11927..>13015)
                     /locus_tag="PLN77_04045"
                     /inference="COORDINATES: protein motif:HMM:NF039514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HRM43487.1"
                     /translation="LVRYGRYSRAFALTDRYGYEKIPVSCLFRLAHAMILEKEFTEDE
                     ELLYLASYVVKQGIYDEIVLCYLRDYFTGSIEDMCSLWDKIRGFQMESDKLEERILTW
                     SVFVRSHPKWEQEMLESYIRQSGKEHVILAYLTYLAIGYFMDGKPFSDQMFDYLDRAW
                     KQGWELDEICHLAMLKHLSTKPQLSEEEEKEAHTLLLAFTKQGLRFAFYKDLPEHLTE
                     GFQIGDRMFIEERQRAEAKVTIHYRTNAEETWKSEPMRNMYQGIFVKEFLLFYGEKME
                     YYLTFTNRQGEGKTEVKTVSMTGEPKAGKTRYQLLNQMKAARAAGDSEKAEEAFQKYL
                     QQEALVRNCFALVDEKKQQEDTTWNRKI"
BASE COUNT         2560 a         3492 c         3156 g         3807 t
ORIGIN      
        1 tagtttgctt tctgtatttt gcttttttct atttttctgc cagaaagctc tctgcaacga
       61 gcagatcctc cggtgttgtg atttttatat tttcatagcc gccttttacc atcactgatg
      121 cgattcccat ctcctgctcc acaaccatgg catcgtcggt gattccttct gtggaatgct
      181 gcatcagttt ttcgaatgcc ggaaacagaa gctctgctgc aaaggcctgc ggggtctgtg
      241 cctgccatac gagatctctt cgcgggcttt ccctgatttt tccgttttca tcggtaagct
      301 tgatggtatc cttgaccggc atgccaagga cacaggcttt ttcttctttt acacatgcgc
      361 agagacgttc cagcatttcc tggtttaaaa acgggcgggc gccatcgtga atcagaacgt
      421 aatcggtctt ctgctcctgt tcgctttcca ggcttttcag cgtctgcaat gctttccaga
      481 cagattcata ccgttccctt ccgcctgcgc atacggaaac agttttctta attccatatt
      541 tttcgataat ctctttctga cagtattcta ttttatccgc acctgtcaca agaatgatct
      601 ggtcaatctc cggcgaatgt tcaaaaacct ccagcgcgta aaccacgact ggttttcctc
      661 cgatttccag aaactgtttt gctacgcttc cgcccatccg ggttcccttt ccggctgcca
      721 ggacaaccgc agtgtatttt tctttcttct cgttcatctt ttcctccgtt ttctacaaat
      781 acaaaaactc cgcagcagat ctctcttctc cgttctgctg cggaagtttc tttttcttat
      841 cgttctccaa gcttcaggtt cggtacggca ttcaggtccc agccgtggcg ggttcccttc
      901 tggtactcgt agtaagctgc cacaccgatc atggccgcat tgtcggtaca gaagatcggg
      961 gacggacggt aaaagcggat gccctctttt tcacatgctg cttccatcgc cgcgcggagt
     1021 gccccgttcg atgcaacgcc gccggcgatc gccagacggt ccatatggta atctttggcc
     1081 gcacggatgg cattatccac cagaacgtcc acaacggcct gctggaagga cgcagcgatg
     1141 tctgcttcga caattggttc gcctgtcatc cggcatttgt tcagatggtt taaaacggca
     1201 gattttacgc cgctgaagct gaagtcatac ggcgcatcct ccatgtgggc acgcgggaag
     1261 tcgatggcgt gcgggtttcc ttcttttgcc agcttgtcga tcttcggtcc gcccggatag
     1321 ccgagtccga tggcacgggc taccttgtcg aaggcttctc cggctgcgtc gtctctcgtt
     1381 cttccgagga tttcgaaaga accgtaatct tttacaatga caagatgggt atggcctccg
     1441 gagatgattt cacacaggaa cggcggctcc agatccgggt gttcgatata gttcgccgag
     1501 acgtggcctt caatatggtg aacgccgacc agcggtttct tttttgcata ggcgatcgct
     1561 tttgcctctg ccacgccgac gagaagtgcg ccgacaagac cagggccgta cgtaacaccg
     1621 atcgcatcga tatcatccag agtgacatcg gcttctttta aggccgcttc tacaacctgg
     1681 ttgatttttt cgatatgttt tctggatgca atttccggca caacgccgcc atacagggta
     1741 tggatggcga tctgggatga aataatgtta gacagcacac atcttccgtt tttcacgacg
     1801 gaggctgcgg tttcatcgca ggagctctca attgcaagaa tcagggtatc tttttcactc
     1861 atttttcgtt tcctcttcaa ataacagttc tactgatctt ccgtcttttc caagctctgc
     1921 ggccgggaaa atcttctgta cctcatccat gtaccgctgc gggccgacga gcgacgggga
     1981 aaagtgggtc agccacattt cggcagcctc ggcatctttt gccagttttg ccgcctcgta
     2041 gaaggtcata tgcttaaatt cttttgcttt tgcctgtttt tccggctcgc cgtacattcc
     2101 ctcgcagatg aaaaggtcgg agcctttcgc atgttcggag atcgacggaa tcgggcgggt
     2161 atcggtgcag taggtcacct tcagtccctt tctggcaggg ccgaggacca tgtccggcgt
     2221 atacgtcttt ccatccaggg tgacagtctc gcctttctgc agcggattcc agcatttaat
     2281 cggaatttcc tgtgctttag cgcgctccac atcgaatttt cccttgcggg agatctccag
     2341 tgaatagccg tagcaggtga tattgtgctg cacgcggaag gctgtgattt tgtagccgtt
     2401 catctcgatc acttcctccg gcgctgtcac ctcgtgaaat acaatcggga acggcagctc
     2461 cgggcagatg acacgcagtg aattgacgac gcgctccagg ccttttgggc cgatcatggt
     2521 cagcggttct ctccggtcag tgtttcccat cgtcaggaga agtcctggaa ggccgctgat
     2581 gtggtctgcg tggtaatggg tgaagcagat ggcatcaatc ggcttaaagg tccagccttt
     2641 ttccttgatg gcaacctggg ttccctcgcc gcagtcgatc agaaggctgc ttccgttgta
     2701 tcgggtcatc agggcggtca ggcgtctgcc tggcagaggc atcataccgc ctgttcccag
     2761 taaacataca tctaacacgt ttttcctcca tattctatcc ggcttttttg aaagccggtc
     2821 aggtatttta cgttgaaaaa gcgtgtccaa aacggtttgt ttcagacacg ctctcatctt
     2881 aacacagttt tttcaaacac gcaatgacgc gggttttatt tatttgctgt catgacagct
     2941 tccttcatca tgcggacata cgtatagatg tgctgctccg catgagtgcc gtacttcgca
     3001 atcattttga cgatagcgct tccgacgatg acaccgtccg caatttttgc aatgttggcc
     3061 gcctgatcgg tcgtgttgat gccgaagcct acggctaccg gaatatcggt tactttgcgg
     3121 attgcggcga tcatggaatc gaggtctgtc ttgatctcgc ttctgacacc ggtaacgccc
     3181 agagacgata cgaggtagat aaaaccttcg gcgtctgcgg caattttctg gatgcgctgc
     3241 tcagacgtcg gcgcaatcag ggaaatgaca tctacctgat ggctgcgcgc aatatcgttt
     3301 aattcgcctt tttcctcata cggcatatcc gggatgatga tgccgttgat gccgacttcc
     3361 tcgcattttg taaagaacgg ctcgtatccg taatggaaca ccgggtttaa gtaggtcata
     3421 aacaccagcg gaatgtctgt ttttttgcgc acggaagcta ccatatcgaa aattttatcg
     3481 gtggtggttc cggcttttaa ggaacggagg tctgcctcct ggatgacaac gccctcggcg
     3541 atcggatcgg aaaacgggat gccgatctcg atcaggtctg cgcctgcttt ttcgagctcc
     3601 agaatataat tctctgtttt ttcaatgttc gggtctccgg ctgtcagaaa cggaatgaat
     3661 gctttgccgt ttgaaaatgc ttctgttact ttactcatga aggtccctcc ctctgtatct
     3721 tgcaatggct gctacgtctt tgtctccgcg tccggacagg cagatgatga tggagtcttc
     3781 cggatcaaat tcttttgcga tcttcatcgc atgggctact gcatgggcac tctcgatcgc
     3841 acagatgata ccctcggttc ttgcgaggta ttcaaaagct tctactgcct catcgtccgt
     3901 taccggaacg tattcggcgc ggccgatgtc atgcagatat gcatgctccg gtccaatgcc
     3961 cgggtagtca aggcctgcgg aaatcgagta aaccggtgcg atctgaccgt atttatcctg
     4021 gcagaagtag gatttcatac cgtggaagac gccgagtgtt ccggttgcaa tggttgctgc
     4081 agtctctgcg gtgttgacgc cgcgtcctgc tgcctcgcat ccgatcaggc ggacattttt
     4141 gtcattgatg aagttataga acatacccat cgcattgctt ccgccgccga cgcatgccat
     4201 gacggctttc gggagttttc cttctttttc gaggatctgc tctcttgcct ctttgctgat
     4261 gacagcctgg aaatcgcgga cgatggtcgg gaacggatgc gggcccataa cggaaccgag
     4321 aacgtagtgg gtgtcatcga cacgtgatac ccactcgcgc atcgcctcat tgacggcatc
     4381 ttttaaggtc atagttccgg tagtaacggc atttactttt gcaccgagaa gctccatccg
     4441 gtatacattg agggcctgac ggtcggtgtc ttcttttccc atgaagatct cacactcaag
     4501 tcccatcagc gctgctgccg ttgcggttgc cacaccgtgc tggccggctc cggtctctgc
     4561 gatgacacgg gtttttccca ttctttttgc gaggaggcac tgtccgagca cgttgttgat
     4621 tttgtgggct ccggtgtggt tcagatcctc tcgtttgagg taaatttttg ctccgccgag
     4681 atcctttgtc attttctctg cgtagtagag aagggacggg cggcccgcgt aatttttcag
     4741 caggtcatcg agctcctggc agaactccgg atcgttttta taataattgt actgtttttc
     4801 caaattgata atgtcattca tcagagtttc cggtatgtac tggcctccgt gaataccaaa
     4861 tcttccgtca gacatgtctt atcctcctta tgcattctgt gatttttttc gggtccttta
     4921 cgccgtctgt ctcgacaccg ctgctgatgt cgacggcgaa gggatgtgtc tgtaaaatcg
     4981 cctgttccac attttcggcg ttcaggcctc ccgcaaggaa atacgggcgg tccatgtttc
     5041 tgatcagtga ccagtcgaac gattcgccgg taccgccttt tccgtggtcg agaaggatgt
     5101 aatcggcgct gctttttttc gccttttcga cgtcctccct ggttttgatg gaaaatgcct
     5161 tgatcagttc cccgccggtg cggaaccgga gcgaatggat atattcttca ctctcatttc
     5221 cgtggagctg tgccagatcg atgacgccct cttcgaggta tcctgcgaca atcgccggaa
     5281 gctcgttcac aaagacgccg accgcctgga tttcgggatc cagatagcgc cgcagcgtca
     5341 cggcatgttc ttttgtaatc tgtctgcggc tttttgcaaa gacaaagccg acatattcgg
     5401 gcttcagctc attcaccgca tcgatgtctt ccagccgtgt gagcccgcag attttgattc
     5461 tggtctgttc ttccataacg tttgactccc cgtgcgctct gttttgtttc gcacacttac
     5521 gttaccactt taacacattg aagagagttt ttcaagctct gcttttttgt tgctgctccg
     5581 catcagcgtt tcgccgatca gaacggcatc ggttttattc tgatacagtc ttccgacatc
     5641 ctccggcgtt ttgatgccgc tctcggatac gaaaacaatg ttttccggga caagctcccg
     5701 cagccgctca ctgttgtgga tatcgaccgt gaaatctttt aagttgcggt tgttgacgcc
     5761 aatgatcctt gctccggcgc gcatggcctg tgcaacctcc gcctcatcgt gggcttccac
     5821 aagagcggaa aggccgaggg aatgcgctgt ttccagatat tcttttaagg tttcctccgg
     5881 cagcagcgca cagatcagaa ggacagcggt ggctccgagc acctttgcct cgtagatcat
     5941 atagggatcc acggtaaaat ctttccgcag aaccgggatg gatacggttt ctgcaatttt
     6001 ttttaagtaa cgcttgtcgc ctttgaagta ttccggctcg gtcaggcagg aaatggcgga
     6061 ggcaccggcc gcttcatatt cctttgcgat ctccagatag gggaaatctt ctgcgatgat
     6121 gcccttcgac ggggaggctt tttttacctc gcagataaag gacatgcccg gctttttcag
     6181 cgcctgttca aacgggaatc cggtgtccgg gttcatcgcc gttgcctttt ctttcatttc
     6241 tgcaagagaa cacttctctt tttctgcagc gacacgtttt cttgtggctt ctgcaatcgt
     6301 ttccagtatc atatgtttat tccttttctg ccaatttgtt ggtgagtgac acaaactctt
     6361 cgagtttttt ctgtgctttt ccgctgtcga tcagctcctc tgccagtttt acagcatcct
     6421 gcatcgggat ttcttttgct acatgaaggg ctgcggcggc attcaggaca acagcgtctc
     6481 ttaccgggcc tttctttcct gcaagaattt ctttcgtaat ttcagcattc tcctgcggag
     6541 ttccgccgac cagctcttct ttggtgcagc gggtcatgcc gaactcttcc ggtgtgatga
     6601 cgtattcctc aaacgttccg tccttgatct cgcagacgct tgtcggtgcg cttaaggaaa
     6661 tttcatcgag gcagtcctgg ccgtagacaa ccatgccgcg tttgacacca agattggaga
     6721 gaacttctgc catcgggcgg acaagtgctt cctcgtagac acccatcacc tgcatcgtgg
     6781 ctgctgccgg gttggtcagc ggtccgagga tgttgaaaat ggtccggatg ccgagcatct
     6841 tgcggaccgg tgccacgtat ttcatggaca tgtggtattt ctgtgcgaac aggaaacaga
     6901 ttccaagttt ttccagaact tctttgtttt tctccggctc caggtcgatt ttgacgccga
     6961 gcgcttcgag acagtcggct gcgccggatt tgctcgatgc ggcgcggttt ccgtgttttg
     7021 caactgcgac gccgcctgca gagatcacaa gggatgctgt tgtggagatg ttgaaggaat
     7081 tggatttatc tccgccggtt ccgacgattt ccagaacatc cttgtcatgt tccatgtgaa
     7141 caccgtgctt tctcatgccg ttggcacatg cggtgatctc ttcgattgtc tcgcctttca
     7201 tcgtcatggc agttaaaaat gcgctgatct ggatcggatc ggcttttcct cccatgattt
     7261 catccatggc gccctctgcc atctcgtagg ttaagttctc tttatttacc agttttacaa
     7321 tcgcttcccg aatcatattc tgtttctctc tttctttgct gtcctgtgat ctatcttgta
     7381 atttttaaga agttttctac catctgtctg ccctgcgggg tcagaatgga ttccggatgg
     7441 aactggactc cgtagatctt tttctcactg tcctcgactg ccatgacctc gccgtctgca
     7501 gttcttgccg ttacctttaa ggtttccggc agtgtcgctt catcagctgc gagggaatgg
     7561 tatcttgcaa ccggaacggt ttccggcagc ccctggaata attccgaggt aagatccagc
     7621 gttgtctcgg actgtttgcc gtgcatcagc tgtttcgcat aggtgacggt cgcgccgtac
     7681 gcggcacaga ttgcctgatg gccgaggcag acaccgagga tcgggatctc gccgcctaat
     7741 ttttctacaa tctccatgca tcggcctgcc tgctccggac ggcccggtcc gggagataag
     7801 atgatggatg ccggtgccag tgcgcagatt tcgtcgatcg tcatctcgtc gttccggatg
     7861 accttcatgt ccggattgat cgaaccggcg agctgataca ggttatagga aaaactgtca
     7921 taattatcta ccagcagtat cataagttgc ttcctccttc tgcctccgca agtgcgacca
     7981 tcacggcctt tgctttgttg atgcattcct gatgctcttt ttccggtacg ctgtcggcta
     8041 caatgccggc accggagcgg acgaacactt ttccgttttt cttaaacgca agccggattg
     8101 cgatgcaggt gtccatattt ccggtgaaat ccagatagcc gatcgcaccg ccgtaaatgc
     8161 cgcgcttgtt gttttccagc tcgttgatga tctggcaggc acgaagcttt ggtgcaccgg
     8221 agagggttcc ggccgggaga acggaatcga tggcatcgac tgcggtcttg tctttccgaa
     8281 gctgtccttt tacagtggaa ccgatgtgca tgacgtggga gtaacgttcg attgacatgt
     8341 atttttctac ttccacggtt ccgaaatcac agatctttcc gagatcgttt cttcccagat
     8401 caaccagcat gttgtgctcg gcacgctctt tttcatcggc gaagagttct ttttccagcc
     8461 gctgatcttc ttcatagctc tttcctctcg accgcgttcc ggcaagcggg aaggtccgga
     8521 cctctccgtc ctcgactttt acgagagttt ccggggatgc accggcgatc tcgacatcat
     8581 cactcgagaa gtagaacatg tacggcgacg ggttcgtcgt gcggaggacg cggtacgtgt
     8641 cgaggaggct tccctcaaaa tcagcttcga gccggttgga gagaacgagc tggaagaggt
     8701 ctccctcgtg gatataatgt ttgacctgct cgactttctc acagtacgct ttctcatcga
     8761 agagcgggcg gaactcggat ttcaggtggc ctgctttcgt ctcggccggt tttccgaagc
     8821 ggatcagctc ttccattttt tcgagttcct gtacggcttt ttcgtaattt tcttcgagat
     8881 tttcggtttc gatattaatc atcagtacga ttttctgacg gtaattatca taggcgatga
     8941 ccttatcgaa cagcatcagg tcgacatctt tgaaatgttc ctgatcttct gcgtccagct
     9001 ttaaggtcgg ctcgctgtac ttgatgtaat cgtaagagaa gtatccgacg aggccgccgg
     9061 tgaaggccgg cagtgtcgcg atcttcggac ttttattttt ctcaatcagg gttttgatga
     9121 cggctcccgg atgggctgtc tttgcggtca gccggatctg tccggtcgga agctgttctt
     9181 tgtggctttc cggaatatct tctacttttt tcatctctgc cgcatcggcg gtgattgtca
     9241 ggtttccgtt gacacaggtg agttccagcg acggatcgta gccgagaaag gtgtatcttc
     9301 cccactgttt tttatcctcc acgctttcga gcatgtagca gtggctgctg acgccctgca
     9361 ggactttcag tgtttctact ggtgtacgga tatccgaata aatttctctg cttaccggga
     9421 ttttccgaaa agctcccgat acggctgctt ttgaaatttc ttctaacgtc atctgtttca
     9481 tcttgttatc tcctctcctt gtgtctgcaa aggctgtcat caacactggt cctcagaacc
     9541 aatttctgat atacaaaaaa ccgcccccgg caaagaatca ctttgttctt tgccaagggc
     9601 gggtatattt ttatactcgt ggtgccacct tgttttgcga ttgctcacac gctctgcaga
     9661 atacgatcat attcctggca actaacgtat gcctgcacgt tacagaatac tcagacggtt
     9721 tcccgctttt ccctgtaccc tccgtggtcc atttgctgat ccgctttctg tccggcttcc
     9781 acctgcccgg gctctctgtg agtgcgcttc caacgttatc tccacttcat cggtttcgaa
     9841 gtatttattt ttttcttatg cgctgtatta tagtcccatt cttttttgct gtcaagtaat
     9901 ttttttgttc tttttgttca aagtccggta tttttatgat attcccgtgt ccacgccgca
     9961 agagcttcgt ccagatcgcg cgggagctcg acctgcattg cctcggtgat ctggcggtcc
    10021 atctcccgtt tcgtctcggc atccactttc cgctcggtgc acatctgctc ataggcgtcc
    10081 tgtcctttgc tcatgtagac cgcaaacagc cagctcttta acgatgcttt cgtgtgcagc
    10141 tcttcatttg ctttctgaaa gcacgtaagt gcttcattca tctggaacag acttgcgtag
    10201 gcacagccca tgttgttgta aatacttccg gtgaactggc ttcccatgtt ggtttcgttt
    10261 tcctcggtat ctttcagcgt ttcctggtaa acctggatcg cattgatata tttttcgtgg
    10321 tcgacaaggt agtctccctt gatttttttc cgcacaatct ccggctgctc ctcgaaccgt
    10381 ttcaggcgct cattcatctc acggaactga tcgtgggaaa gataattgat ctccttgaag
    10441 atcggcagga taaactcacc gatgttcttt ttctcctcca gaaggacgta gagacgctgg
    10501 tacagtcttc ttaagtggag ttcttctttc atccacacca gcagctgctc gttaatgatg
    10561 gtctcgtcca gcagatagat gttgtggtac agataatagc acagttcctc gatggaataa
    10621 atatttgtgc tgatgttgga aatataatac ggatttttcg cccgtttgac ctgacataaa
    10681 atataaccgc tcatcgttta cacctccccc atcgtctcgt gccagatttt gtggcttgcc
    10741 gggaacatat cgccaaaacc taaatcctct gcggtgatct gacacgtaac cggagaatca
    10801 tactccagac gcagacggat ccgtgttgtc ttcggcggcc gtttgggcag cccgtccagc
    10861 ttcatggtat agcggtttcg ttttccgctt tccatggagc ttacaacaaa ttccagctcc
    10921 tccgttccgt cgaggatcag ctcacattct ttttctgctt cataccagtt cacaccggct
    10981 gcaatcagcg catagtatgc cggagaacca ttgatcgtca tctccatgcc gatgttgtag
    11041 cgcacgaggt catttccgag gaagagatag ccctttaagc ggcgctcctc cactttttcg
    11101 cgcgcggaaa agcaggcgcc ctgtgcaaac aggttcgttc cgccgaagat tcttctctgg
    11161 tttttcttta actgacgcag agagttgtcg gcccaggcaa ggtcgaactc ctcgctcacc
    11221 agaaatacgc tggaataaat ttcattcccc atcgcttctc ccatcagatg gcagaaatcg
    11281 cggtcgcgct ccatcggctc gatcgaaagt gcggcttttg gcccccgttt tacggtcaca
    11341 gttgccggct tcgttttacg gctgatggaa agttcagaaa acgatgcttc ctcatctttc
    11401 aggaaaaaca gaccgacttt acggctccag agctccggct tctggttcag cacatggcag
    11461 taaaagctct cgtcatgctc ctgcaggtag gctctgccgc gcggaagtcc gattatcttg
    11521 tatgcttcac gcacattttc cacaaatgga cgggtcagat ggcccgtggt gatcatcatc
    11581 gcctggatct gctggtccgg gcgctcgatg ccgatcatct tcagtgcttc gcgaaggaac
    11641 gtgccaagga cttctcccgg cgtgtaatcc cggttttcga tctggcagat gcgggatccc
    11701 atgcagatct cgtacagatt gtccagccag atgccgtttt ccgcgtgagc agatttttct
    11761 gcatccggtc ctgttttcca cgtttcctca ttccgttttt tggcaatggc ggtcggaaat
    11821 tccaccgcct cttctccttc ttttaccggg atggagacgg ccgtatgggt tcccctgtca
    11881 tagcagcaga actgggaata tttctctcca aaatcaaatc ctaaaatcaa atttttctgt
    11941 tccatgtcgt atcctcctgc tgcttctttt catccacaag tgcaaaacag ttccgaacca
    12001 gagcctcctg ctgcagatac ttttggaatg cttcttctgc tttttccgaa tctcctgccg
    12061 cgcgcgctgc tttcatctga ttcagaagct ggtaccgcgt ctttcctgct tttggttcgc
    12121 cggtcatgga gacggttttc acctctgttt ttccctcacc ctgacggttc gtaaaggtca
    12181 ggtaatactc cattttctcg ccgtagaaca gcagaaactc tttcacaaaa atgccctgat
    12241 acatattgcg catcggttcg ctcttccatg tttcttcggc gtttgtccgg taatgaatcg
    12301 tcacctttgc ctccgctctc tggcgttcct cgatgaacat gcggtcgccg atctggaagc
    12361 cttcggtcag gtgctccggc agatccttgt agaaggcaaa gcggagtccc tgtttcgtaa
    12421 acgccagaag cagggtatgc gcctcttttt cttcctcctc agaaagctgc ggcttcgtgg
    12481 aaagatgttt cagcatcgca agatggcaga tctcgtccag ctcccagccc tgtttccagg
    12541 cgcggtccaa gtagtcaaac atctgatccg aaaacggttt tccgtccata aaatagccga
    12601 tcgcaagata cgtcaggtat gccagaatga catgctcttt tccgctctgg cggatgtagc
    12661 tctccagcat ttcctgctcc cacttcgggt ggctgcgcac aaagacggac caggtcagaa
    12721 tgcgctcctc cagcttgtcg ctctccatct ggaagccgcg gattttatcc cataaactgc
    12781 acatatcctc aatagaaccg gtgaaataat ctctcagata gcacaataca atttcatcgt
    12841 agatgccctg ttttaccacg taggatgcca gatagagaag ctcctcgtcc tctgtaaact
    12901 ctttttccag aatcatcgca tgcgccagac ggaagagaca cgacaccgga atcttctcgt
    12961 agccgtaccg atccgtcagg gcaaaggcgc ggctgtaacg gccataacgc accag
//
LOCUS       DAOMUF010000043        12867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1096_length_12867_cov_3.493600, whole genome shotgun sequence.
ACCESSION   DAOMUF010000043 DAOMUF010000000
VERSION     DAOMUF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_12867_cov_3.493600"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2132
                     /locus_tag="PLN77_04050"
     CDS             <1..2132
                     /locus_tag="PLN77_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HRM43488.1"
                     /translation="IEIPEFTEEEEEIEETPAETKTESEPEEVMVDDDLIINDPFADE
                     DDLLGEEKTETKGSEKEVLGDQVEAAMERLVSDTVGHIPMPEIDDEDDDYDDYEDDDE
                     EYIPHGAKPQKKKGKGGAVIAVAAVAVIAVGGFFYGSKMFHYQNYFFNGTTINGVDCS
                     DMTAKEAETKIKEQAANYTLNVKFKDNDTKSVSGKDIDYTYAGDGSVESVLKKQNSFL
                     WFLGNGGKEKDSQVTMQYSQEKLDAVIDGFDEFQSADGENAPASAYITFQNNEFEIVD
                     AVMGEGMDLTLAKQCIENALINADTEVDLEKAGVYDGTLVTADDETLNAQKDQLNELV
                     RASITYSMPDGTTQVLDGNTMKDWLAVDADGNYSKDENQWNERVKEYVANLAAAIDTD
                     GKDHTFPATGIEGGVTISQEGYGWKVDQEQEIAKIAEEVDAHAADAREPQYAQREFAA
                     STENNGFGKTYVEVDASRQHIWLYKDGNLVVDGDCVTGLMEQSSYTKPGIYTTAAKES
                     QKKLHGELQADGSYSWERDVDSWIPFNGEIGFYDASWRSSFGGNLYLTAGSTTGSVAL
                     PTAVAQALYDNVDDGTPVIIYYSEAYEVSEDTLTVTQAPEADDENVDDTTNTTTVTPT
                     RTPSYTYDDYTPSTPSTPSTPSTPSTPSTPSTPEPTTAPTEIPSTPEPTVAPTETPST
                     PEPTQEPSAPDQGDHTGDDDYPGKGES"
     gene            complement(2219..2851)
                     /locus_tag="PLN77_04055"
     CDS             complement(2219..2851)
                     /locus_tag="PLN77_04055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM43489.1"
                     /translation="MIDSIIFDVDGTLWDSTDVVADAWNQIMEKETDMTPNLTGAVLK
                     GLFGRLLPDIAAVIFHEYPKERQLELIEACCAKEHEALLATPAPVYPGLVDALSILSR
                     HYPLYIVSNCQAGYIEVFLEATGLGHYFKGHLCPGDTGKAKADNIRTIIEQNHLQHAV
                     YVGDTDGDYKATREAGIPFVHAAYGFGQTENPDYTITSPMDLVTLFVPEN"
     gene            3000..4067
                     /gene="mutY"
                     /locus_tag="PLN77_04060"
     CDS             3000..4067
                     /gene="mutY"
                     /locus_tag="PLN77_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRM43490.1"
                     /translation="MQDTITKPLLKWYGENKRVLPWREKKNPYEIWVSEIMLQQTRVE
                     AVKPFYERFMRELPNVAALAVCPEEKLLKLWEGLGYYNRVRNMQKAAQKIMEVYDGVF
                     PADYEALKGLPGIGNYTAGAVASIAFCIPVPAVDGNVLRVMARLREDGEDILKQSVKN
                     RVEAELTEIMPAEDPGAFNQAMMDLGAMVCLPNGAPKCEVCPLFDQCLAGQHQTWTEY
                     PFKKSAKPRRIEDRTVLLFLDGAHTAVRKRPKKGLLAGLYEFPNFDGVLSEQEALEEA
                     EKFGVTPLHIQALPPYKHIFSHVEWHITGYAIKIAGNDVEQEGEEKAGLIFADAKTAA
                     ERYAIPSAFAEYAKYTDLERA"
     gene            4105..5121
                     /locus_tag="PLN77_04065"
     CDS             4105..5121
                     /locus_tag="PLN77_04065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM43491.1"
                     /translation="MKILSIAVPCYNSEAYMEKCIDSLLVGGEEVEILIVDDGSKDGT
                     TEIADRYQEKYPTIVKAIHQENKGHGGAVNTGVENATGLYFKVVDSDDWVNPEAYQKI
                     LNVLAEVVRGPKTLDLLISNYVYEKEGAKRKRVMRYAKSLPEGRFFGWDEAKALGKTH
                     YLLMHSLIYRTSLLRECGMKLPEHTFYVDNLVAFIPLPYVKTMYYLDVNFYRYFIGRA
                     DQSVNEKVMIGRIDQQIRVNKLMIDYLGEQKGLSKYLRKYMISYLTIIMTVSSVMMMR
                     SGTEENLEKKNDLWRYLKQEDAADYFRIRHGIFGTVMSSKSKPGQKIAVYAYKVAQKL
                     YGFN"
     gene            5148..5696
                     /gene="ispF"
                     /locus_tag="PLN77_04070"
     CDS             5148..5696
                     /gene="ispF"
                     /locus_tag="PLN77_04070"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRM43492.1"
                     /translation="MRVGMGYDVHKLVENRKLILGGVEIPYEYGLLGHSDADVLVHAI
                     MDALLGAAALRDIGRHFPDNDPAYEGADSIELLKKVGEMLEERMYLIENIDATVIAQK
                     PKLLPYIDTMVKNVAEALHLEEDQVNIKATTEEGLGFTGKKEGISAQAICCISKALDL
                     MPDDRIAGGCSGCPGCAKNAEE"
     gene            5959..8586
                     /locus_tag="PLN77_04075"
     CDS             5959..8586
                     /locus_tag="PLN77_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRM43493.1"
                     /translation="MKEYLEKSSKILDELQTTEQGLSEAEAARRLSEHGPNRLAAGKK
                     KSNLQRFFEELKDPMILILLAAALISGITSAYEGESYADVVIILTVVIINAVLGVLQE
                     SKAEKAIEALQEIAAATSKVMRGGKMEVKKSEELVPGDIVILEAGDAVPADGRILESA
                     SMKIEEAALTGESVPVNKTADVLNAGAASEVPLGDRKNMVYMGSTVVYGRGRAVITGT
                     GMKTEMGKIAHVLTQSADESTPLQKRLNQLSKILSVMVLAICAVIFIVGILKEGVTPQ
                     NILNTFMVAVSLAVAAIPEGLAAVVTIVLSIGVTKMSKRNAVIRKLTAVETLGCTQVI
                     CSDKTGTLTQNKMTVVKHEGSDIKRLVSVMALCSDAEPDENGEAVGEPTECALVNDAQ
                     KEGCPKKSLSVQYPRVGEAPFDSMRKRMTTIHRANDGTYFSCTKGAPDEILKRCTSVW
                     RDGRKQPMTEAFRQEILSQNKAMADQALRVLAAAGRAWNQMPSSQEPDFLEQDLCFLG
                     LCGMIDPVRPEVVDAIRECRGAGIRPVMITGDHKDTAVAIAKELGILEAGWKAVTGAE
                     IDQLSDEELDRRIEEYAVYARVQPEHKVRIVDAWKRKGRITAMTGDGVNDAPSIKRAD
                     IGIGMGITGTDVTKNVADMVLADDNFATIIAAVEEGRRIYDNIRKAIQFLLASNLAEV
                     LSIFCATLMGFTILNPVHLLWINLITDCFPALALGMEDGEADIMKRPPRNADDGIFAD
                     GLGFDVAFQGIVITVLTLASYFIGHYLEAGRWEIVNSPDGMSMAFLTLSMVEIFHSFN
                     MRSRKGSIFHMKHQNKYLWGAMALSFVLTSAVIYIPVLSSAFGFERISALEYDVAIGL
                     GLLVIPAVEISKWIRRKFA"
     misc_binding    8629..8863
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8966..9649
                     /gene="cysE"
                     /locus_tag="PLN77_04080"
     CDS             8966..9649
                     /gene="cysE"
                     /locus_tag="PLN77_04080"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRM43494.1"
                     /translation="MSFFAYFKEEFQVIQERDPAIHSPMEVLLYPSFRVMLKYRRAHK
                     LYLKGHYFLARWISQRAARKTGIEIHPGATIGKGLFIDHGAGVIIGETTVIGDNVTLY
                     QGVTLGGNGKETGKRHPTLEDNVMVSAGAKIIGSFTIGENSKIGAGSVVIEEVPPNCT
                     VVGVPGHIVKQNDVRIPRKSMDHIHLPDPIFEDIKVLQQENTELTNRVLELEGELRQM
                     RKKERTEQK"
     gene            9757..10116
                     /locus_tag="PLN77_04085"
     CDS             9757..10116
                     /locus_tag="PLN77_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRM43495.1"
                     /translation="MYSPLTLAYIGDAAYEIVIRTILVRKANMQVNKLHRHAAGLVKA
                     EKQSAMIEILEPLFTEEEKQIYKRGRNAKSYTKAKNASTIDYRRATGFEAVMGYLYLK
                     GDYKRMIDLIRAGLGEV"
     gene            10360..11082
                     /gene="rlmB"
                     /locus_tag="PLN77_04090"
     CDS             10360..11082
                     /gene="rlmB"
                     /locus_tag="PLN77_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRM43496.1"
                     /translation="MVEGRNAVIEAFRSGKTVDKLFVLASSQDGPIRTIIREAKKHDT
                     IINYVEKERLDELSTTGKHQGVIAQVASYSYATVEDILARAEEKGEPPFILLLDGIED
                     PHNLGAIIRTANLAGAHGVIVPKHRAVGLTATVAKTSAGALNYTPVAKVTNLVQTMEM
                     LKEKGIWFVCADMNGQRMYDLDLKGPIGLVIGNEGEGVSRLVRENCDFTATIPMKGDI
                     DSLNASVAAGVLAYEIVRQRSC"
     gene            11122..11727
                     /gene="sigH"
                     /locus_tag="PLN77_04095"
     CDS             11122..11727
                     /gene="sigH"
                     /locus_tag="PLN77_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HRM43497.1"
                     /translation="MKDTRDMMVKDYDTCTDEELIARLRAGEREITDYLIDKYKSLVR
                     TRARALYLVGGDHEDLIQEGMLGLFKAVRDYKPGKEASFATFAGLCIDRQMYSAVASS
                     QRQKHQPLNSFVSLSEPVSEQELRLVDEETPEEIMISRENVIGMHERIKERLSKFEYQ
                     VLELYLKGYDYTQIAEKLGKQPKAIDNALQRIRSKVREVCS"
     gene            11898..11970
                     /locus_tag="PLN77_04100"
     tRNA            11898..11970
                     /locus_tag="PLN77_04100"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11931..11933,aa:Thr,seq:ggt)
     gene            12104..12706
                     /locus_tag="PLN77_04105"
     CDS             12104..12706
                     /locus_tag="PLN77_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43498.1"
                     /translation="MGERKLEYKDSKGQDCYFYYSNIAGTDLDMPFYVCNAECVSGMK
                     KRESKSETFSANPFEYEFLYAPLGDKGAYAYQQPWYGFSVNKDSEEKEIAVEFLRFMM
                     QEKEIDEMASIKGLPSVAENGTDERYAGLRDAKNIEASFINDGSVPDSIRAALEQACT
                     AMQTRQCRCLWSCVQRNKIGKREERLICENKWVAFWCQRW"
     gene            12743..>12867
                     /locus_tag="PLN77_04110"
     CDS             12743..>12867
                     /locus_tag="PLN77_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM43499.1"
                     /translation="MESKSKVLQKLFLSTLYLSAFTFGGGYVIVTLMKKKFVDEY"
BASE COUNT         3722 a         2746 c         3734 g         2665 t
ORIGIN      
        1 acatcgagat tccggaattt acagaagagg aagaagaaat cgaagagacg ccggcggaga
       61 caaaaacaga atcggaaccg gaagaagtga tggtggatga tgatctgatc atcaacgatc
      121 cattcgcaga tgaggatgat ctgctgggag aagagaagac agagaccaag ggttcggaga
      181 aagaagtgct tggtgatcag gtggaagccg caatggagcg cctcgtcagc gacacggtag
      241 gccatatccc aatgccggaa atagacgatg aggatgatga ctacgacgat tatgaggatg
      301 acgacgaaga gtacattccg catggcgcaa aaccgcagaa gaaaaaggga aaaggcggtg
      361 cggttatcgc cgttgcagcc gttgctgtta ttgctgtggg aggctttttc tatggaagca
      421 aaatgttcca ttaccagaat tattttttca acggaacaac catcaacgga gtagactgct
      481 cggatatgac ggccaaagag gcagaaacga aaatcaaaga gcaggctgca aactatacct
      541 taaatgtaaa attcaaagac aatgatacga agagtgtcag cggaaaagac atcgactaca
      601 cctacgccgg agacggcagc gtggagagcg tgttaaagaa acagaacagt ttcctctggt
      661 tcctgggaaa tggcggaaaa gagaaagatt cccaggtcac catgcagtac agtcaggaaa
      721 aactggatgc ggtgattgat ggatttgacg aattccagtc cgcagacggt gagaatgcac
      781 cggccagcgc ctacatcacc ttccagaaca atgagtttga gattgtggat gctgttatgg
      841 gtgagggaat ggatcttacc cttgcaaaac agtgcatcga aaacgcgctg atcaatgcgg
      901 ataccgaagt ggatcttgaa aaagcaggcg tttacgacgg aaccttggta acagcagatg
      961 acgaaacact gaatgcacag aaggatcagt taaatgaact ggtacgcgca agcatcacat
     1021 acagtatgcc ggatggaaca acacaggttc ttgacggaaa taccatgaaa gactggctgg
     1081 cagtcgatgc ggatggaaat tattccaaag atgaaaacca gtggaatgaa agagtaaaag
     1141 aatatgtggc aaatcttgcg gcagcgattg ataccgatgg caaggatcac accttcccgg
     1201 caaccggtat cgaaggcggc gtgaccatca gccaggaagg ctacggatgg aaagtggatc
     1261 aggagcagga gattgcaaag atcgcagaag aagtagatgc tcacgcagca gatgcaagag
     1321 agccgcagta tgcgcagaga gagtttgctg cttccacaga gaacaacgga ttcggcaaga
     1381 cgtatgttga ggttgatgcc agccgtcagc atatctggct ctacaaagat ggaaatctgg
     1441 ttgtcgacgg tgactgtgta accggtctga tggagcagag cagctacacg aagccgggta
     1501 tctacacaac cgcagcaaaa gaaagccaga aaaaactgca cggagagctt caggcagacg
     1561 gcagctattc ctgggaaaga gatgtggatt cctggattcc gttcaacgga gagattggtt
     1621 tctacgatgc ttcctggaga agtagtttcg gcggaaacct gtatctgact gcaggatcga
     1681 cgacaggatc tgttgcactt ccgactgcag tggcacaggc attatatgac aatgtcgatg
     1741 acggaacacc ggttatcatt tattattccg aagcttatga ggtaagtgaa gatacgctta
     1801 ctgtgacaca ggcaccggaa gcagatgacg aaaatgtcga tgatacgacg aataccacga
     1861 cagtaacgcc gacgagaacg ccatcctaca cgtacgatga ttacacgccg tcaactccgt
     1921 caaccccatc aactccatca accccatcaa ctccatcgac cccgtctacg ccggaaccga
     1981 ccacagcacc gaccgagatc ccgtctacgc cggagccgac cgtagcaccg acggagaccc
     2041 cgtccacacc ggaaccgacc caggagccga gcgcaccgga tcagggagac catacgggtg
     2101 acgatgatta tccaggtaag ggcgagagct gagaaaagaa gaaatagaac ataaaaaata
     2161 cgcattccgt tatcaaaaca ggaatgcgta ttttttatga gaaagaaact gttaaaaatt
     2221 aattttccgg tacgaacagt gtgacaagat ccatcgggga agtgatggtg tagtccgggt
     2281 tttcggtctg accaaaaccg tatgcggcat ggacaaacgg gattccggcc tcgcgtgtcg
     2341 ccttgtagtc gccgtcggta tcgccgacat agaccgcgtg ctgcagatgg ttctgttcga
     2401 tgattgtacg gatgttgtct gcctttgcct ttccggtatc gccaggacag agatggcctt
     2461 tgaaatagtg accgaggcct gtggcttcca gaaaaacttc gatatagccc gcctggcagt
     2521 tgcttacgat ataaagcggg taatgtctgg aaagaataga aagtgcatcg acaagtcccg
     2581 gataaacagg agccggtgtc gcaagaagtg cctcatgctc tttggcacag catgcctcga
     2641 tcagttctaa ctggcgctcc tttggatatt cgtggaaaat gacggcggcg atatccggga
     2701 gcaggcggcc gaacaggcct tttaacacgg ctccggtgag gtttggtgtc atatcagttt
     2761 ctttttccat aatctgattc catgcgtcgg caacaacgtc tgtggagtcc cacagggtgc
     2821 cgtccacatc aaaaataata ctgtcgatca agagtttttc ctccatatct taagggtttt
     2881 ggttattctt ttaaagtatt tattaatttt aatcaaaaac acgtgagagc gcaatgttta
     2941 ttttgcattt tggaggaaaa tgtggcaaaa taagaatgat atattaacag gagattacta
     3001 tgcaggacac aatcacaaag ccattattga aatggtatgg cgaaaataaa agagtgctgc
     3061 cgtggagaga aaaaaagaac ccgtatgaaa tctgggtttc cgagattatg ctccagcaga
     3121 cgagggtgga ggcggtcaag ccgttttatg agcggtttat gcgtgaactg cccaatgtcg
     3181 cagcacttgc ggtctgtccc gaggaaaagc ttttgaagct ctgggaaggc cttggttact
     3241 ataaccgtgt gcggaatatg cagaaggccg cacagaaaat tatggaagtg tatgacggtg
     3301 tatttccggc agattacgaa gccttaaaag gacttccggg aattggaaat tacacagcgg
     3361 gagccgttgc atcgatcgcg ttttgcattc cggtgccggc ggtggacgga aatgtgcttc
     3421 gtgtgatggc acgcctccgg gaggacggtg aggacatttt aaagcagtcc gtgaaaaacc
     3481 gcgtggaagc ggaattgacc gaaatcatgc cggcagaaga tccgggagcc ttcaatcagg
     3541 cgatgatgga tctcggcgca atggtctgtc tgccgaacgg cgcaccaaaa tgtgaagtct
     3601 gtccgctttt tgaccagtgt ctggcgggac agcatcagac ctggacggaa tatccgttta
     3661 aaaagagcgc aaagccgcgc agaatcgaag acagaaccgt tctgttattt ctggacggtg
     3721 cgcacacagc agtgcggaaa cggccgaaaa aaggacttct ggcgggactt tacgagttcc
     3781 cgaacttcga tggcgtgctc tccgaacagg aagcacttga agaggcagaa aaattcggcg
     3841 tgacgccgct tcatattcag gcattgccgc cgtacaagca cattttttcc catgtcgagt
     3901 ggcatatcac aggatacgcc attaaaattg caggaaacga tgtggagcag gaaggggaag
     3961 aaaaagccgg attgattttt gcggatgcga aaaccgcggc tgaaagatat gccattccgt
     4021 ctgcgtttgc ggaatacgca aaatatacag atctggaacg tgcctgaaac ggcagaagag
     4081 caaaaaagag cagggagaaa aaagatgaag attttatcaa ttgcagtacc atgctataat
     4141 tcagaagcat atatggagaa atgtatcgat tcgctgctgg tcggcggaga agaagtagaa
     4201 attctgatcg tggacgatgg ttccaaggac ggaaccacag agattgcaga ccgctatcag
     4261 gaaaaatatc cgacgattgt aaaagcgatt catcaggaaa acaagggcca cggcggagca
     4321 gtcaacacgg gtgtggaaaa tgcgacgggt ctgtacttta aggttgtgga cagtgatgac
     4381 tgggtaaacc cagaggcata ccagaagatt ttaaatgttc tggcagaagt cgtacgcgga
     4441 ccgaaaacac tcgaccttct gatcagtaac tatgtgtatg aaaaagaggg cgccaagaga
     4501 aaacgtgtga tgcgctatgc gaaatcgctg ccggaaggcc gctttttcgg atgggatgag
     4561 gcgaaagctc tcggcaaaac acattatctg ctgatgcatt cgctgattta ccggacttct
     4621 cttttaagag agtgcggcat gaagctgccg gagcacacct tctatgtgga taatctggtt
     4681 gcgtttattc cgcttccgta cgtcaaaacg atgtattatc tcgatgtcaa tttctaccgc
     4741 tactttatcg gaagagccga ccagtcggtt aacgagaaag tcatgatcgg cagaattgac
     4801 cagcagatcc gtgtcaacaa gctgatgatc gattaccttg gtgaacagaa agggctgtcg
     4861 aagtacctgc ggaaatatat gatcagctat ctgaccatta ttatgacggt ttcttccgtg
     4921 atgatgatgc gctccggaac ggaagaaaat ctcgagaaaa agaacgatct gtggagatac
     4981 ctgaaacagg aggatgcagc cgattacttc cgcatccgcc acggcatttt cggaactgtt
     5041 atgagcagca aatcaaaacc gggacagaaa attgcagtct atgcctacaa ggtagcgcag
     5101 aaactgtatg gatttaacta gaaagtaacg aagcggagga acgaacaatg cgagtaggaa
     5161 tgggttacga tgtgcataaa ctggtggaaa accggaaact gattctgggc ggtgtggaaa
     5221 tcccgtatga atatggcctt ctgggacatt ccgatgcaga tgtgctggtc catgccatta
     5281 tggatgcgct tctcggcgct gcagcactgc gggacatcgg acgccatttt ccggacaatg
     5341 atccggcgta tgagggcgca gacagcatcg aactgctgaa aaaagtcggt gagatgctcg
     5401 aggagcggat gtatctgatt gaaaatatcg atgcaaccgt tattgcacag aagccgaagc
     5461 tcctgccata catcgacacg atggtgaaaa atgtggcgga ggcgcttcat ctcgaagagg
     5521 atcaggtcaa tatcaaggca acgaccgagg aaggacttgg ctttaccgga aagaaagagg
     5581 gaatttccgc acaggcgatc tgctgcattt ccaaggcact cgacctgatg ccggacgacc
     5641 ggatcgcagg cggatgcagc ggctgcccgg gatgcgcaaa gaacgcagaa gaataaaaga
     5701 gaaatgcgat tgcacaaggc cggaagatgt ggtagactgg aagaagaata agggagtagt
     5761 cggcggcata cgtgacgcta taaggccaac aaccgaagaa aaagcatttc ttctggcttt
     5821 gtatgaaatg acaagactta tctgtatcaa caaatatggt gcggataggt ctttttctat
     5881 atagaaagag accggtgtac cagtgggaac ttatcacagg aacgcaaaaa gcgttttcag
     5941 aagaaaggac ggatggaaat gaaagagtat ctggaaaaaa gttcgaagat tcttgatgag
     6001 ttacagacaa cggagcaggg actttccgag gcggaagctg cgcggcggct ttccgaacac
     6061 ggaccgaacc ggctggcggc agggaaaaag aagagtaatc tgcagcggtt tttcgaagag
     6121 ctgaaagacc cgatgatcct gatcctgctc gccgcagcgc tgatttccgg catcacatcg
     6181 gcgtatgaag gcgaatcgta cgctgatgtg gtcattattc tcaccgttgt catcattaat
     6241 gcggtgctcg gtgttctgca ggagagcaag gcagagaaag cgattgaagc tctgcaggag
     6301 atcgcggcag ccacgtcgaa ggtgatgcgc ggcgggaaaa tggaagtgaa aaagagcgag
     6361 gagctcgtac ccggtgatat tgtaattctc gaagcgggcg atgctgttcc ggcggatggc
     6421 cgtattttag agagcgccag catgaaaatc gaggaagcag cgctgaccgg agaatctgtc
     6481 ccggtcaata aaacagcgga tgttctgaac gccggcgcag ccagcgaggt tccgctcggg
     6541 gaccggaaaa acatggtcta tatgggaagc accgttgtct atggccgcgg ccgcgctgtt
     6601 atcaccggaa cgggtatgaa aaccgagatg ggaaaaatcg cccatgttct gacgcagtcg
     6661 gcggatgaga gcacaccgct gcagaagcgg ctgaaccagc tcagcaaaat cttaagcgtg
     6721 atggtgctcg ccatctgtgc cgtgattttc attgtcggta ttttaaaaga gggcgtgacg
     6781 ccgcagaaca tcttaaatac ctttatggta gccgtcagcc ttgccgtagc ggcaattccg
     6841 gaaggacttg cagcagttgt cacaatcgtg ctttccatcg gtgtgaccaa aatgtcaaag
     6901 cggaatgcgg tgatccggaa actgacggct gtcgagacgc tcggctgtac gcaggtgatc
     6961 tgctcggata agacaggaac gctgacccag aataagatga ccgtggtaaa acatgaggga
     7021 agcgatataa aacgtctcgt atccgtcatg gcactctgct ccgatgcgga gccggacgaa
     7081 aacggcgagg cagtcggaga accgacggaa tgtgcgttgg tcaacgatgc gcagaaagag
     7141 ggatgcccga agaagagtct gtccgtccag tatccgcgtg tcggggaagc gccgtttgat
     7201 tcgatgcgga aacgcatgac aacgatccac cgcgcaaacg acggaacgta tttttcctgc
     7261 acaaaaggcg caccggatga gattctgaag cggtgcacct ccgtttggcg tgacggcaga
     7321 aaacagccga tgacagaagc attccggcag gaaatcctgt cgcagaacaa ggcgatggca
     7381 gaccaggcac tccgcgtgct ggcggcagca ggaagagcgt ggaaccagat gccgtcctcc
     7441 caggagccgg actttctgga gcaggatctc tgctttttag gactttgcgg tatgattgat
     7501 ccggtgcggc cggaagtagt cgatgcgatc cgggaatgcc gcggggcagg catccgtccg
     7561 gtgatgatta cgggtgacca caaagacaca gcggttgcta ttgcaaaaga gctgggtatt
     7621 ctggaggcgg gctggaaagc ggtaacggga gccgagattg accagttaag cgatgaggag
     7681 cttgaccgga gaatcgagga atatgctgtt tacgcacgcg tccagccgga gcataaagtg
     7741 cggatcgtcg atgcgtggaa gcggaaaggc agaatcaccg cgatgacagg agacggagtc
     7801 aacgatgcgc cgtcgatcaa gcgtgcggat atcggcatcg gaatgggcat cacgggaacg
     7861 gatgtgacga aaaacgtggc agatatggtg ctcgcggacg acaactttgc gacgatcatc
     7921 gcggcggttg aggaaggaag acgaatctac gataatatcc gtaaggcaat tcagttcctt
     7981 ctggcatcga accttgccga ggtgctgtcg attttctgcg cgacgctgat gggctttacg
     8041 attttaaatc cggtgcatct gctctggatc aacctgatta cggactgctt cccggcgctg
     8101 gcgctcggta tggaggacgg tgaggcagac atcatgaaac gcccgccgag aaatgcggat
     8161 gacggtattt ttgcggacgg cctcggcttt gatgttgcgt ttcagggcat cgtgattacg
     8221 gtgctgacgc tggcgtccta cttcatcggc cactatctgg aagccggccg ctgggaaatc
     8281 gtcaacagcc cggacggcat gtcgatggcg tttctgacac tctctatggt ggaaattttc
     8341 cattccttca atatgagatc acggaaaggt tctattttcc atatgaagca tcagaacaag
     8401 tatctgtggg gcgcgatggc actctcattt gtcctcacat ccgccgtcat ttacatcccg
     8461 gtactgtcga gtgcgttcgg ttttgagcgg atcagcgcgt tggaatatga tgtggcgatc
     8521 ggccttggac tgcttgtcat tccggcggtc gaaatcagca agtggatccg gcggaaattt
     8581 gcttgacaaa cgagaaaaat ttgcataaac tagaacaaac ggaaacagaa aaaggcttgg
     8641 aacggaaaaa gtagaaaatc agaattgttt cacagagaat ggcagacaaa tggggctgga
     8701 agctgcctga aacgagggtt tttgaagtgc gtccggaagc tggccgggtg aagcggaaaa
     8761 agtgggtagt ccggcacggg tcctgcacgt tatgcagaag aaaagaagag ggcaggtgtg
     8821 cccttaaagt aaagtggaac cacggagcta tttcgtcttt atcggagatg aaacagctcc
     8881 gtttttgaat attgcccttc gggcaaaaaa ggtggtaaga aataaaaatc tgaaaaagtc
     8941 ccggcaggag gcaaggagag gaaagatgag ttttttcgcg tattttaaag aggaatttca
     9001 ggtgattcag gagcgggatc cggcgataca ttctcccatg gaagtcttgc tgtatccttc
     9061 atttcgtgtt atgctgaagt acagacgggc gcacaagcta tatctgaaag gccattactt
     9121 tctggccaga tggatttcac agcgggcagc gagaaaaacc ggtatcgaaa tccatccggg
     9181 tgcgacgatc ggaaagggac tgtttatcga ccatggagca ggcgttatca tcggagagac
     9241 gaccgtcatc ggagacaacg tgacgctgta tcagggcgtg acgctcggcg gtaatggtaa
     9301 ggagaccggg aaacgtcatc cgacgctgga ggacaatgtt atggtcagcg ccggtgcgaa
     9361 gatcatcgga tcgtttacga tcggggaaaa ctccaaaatc ggagccggat cggttgtgat
     9421 cgaggaggtt ccgccgaact gtaccgtcgt cggcgtgccg gggcacattg tgaagcagaa
     9481 tgatgtgcgg attccgagaa aaagcatgga tcacattcat ctgccggacc cgattttcga
     9541 ggatatcaaa gtactgcagc aggagaatac ggagcttacg aaccgtgtgc tcgagctgga
     9601 gggcgagctg cgtcagatga ggaaaaaaga aaggacagaa cagaagtgac ggaagtgggc
     9661 aaagagacag aaaacgagga gctttttcca gaggcggttt tcggggagaa ggattatctt
     9721 tcggaggtat tcggactgga acagcatgac atccgcatgt attcgccgct tacgctggcc
     9781 tacatcgggg atgctgcgta tgagatcgtc atccgcacga ttctggtgcg gaaagcaaat
     9841 atgcaggtca acaagctgca ccgccatgcg gcagggcttg taaaggcgga gaaacagtcg
     9901 gcgatgattg aaattctgga gccgctgttt accgaagagg aaaaacagat ttataaaaga
     9961 ggcagaaatg cgaaatccta taccaaggca aaaaatgcat ccacgatcga ctaccgccgg
    10021 gcaaccgggt ttgaggccgt gatggggtat ctgtacctga aaggggatta caagcggatg
    10081 atcgacctga tccgggcagg acttggcgaa gtgtgagagc agataagatt cagaaaataa
    10141 aaaagaagcg gcagggcagt ggggcttttg aaaaagcaaa agttcccact gcttttggga
    10201 ccgcggaaca ggaatgatat gagagaaaac aacagaaacg acaagagaaa ctttgagaga
    10261 agagacggaa aaaaacgtga ctttaagggg aaacgagatt accgtgacag agacagaaaa
    10321 caggaagtac agcaggcgga tgaggagttt acaggcggca tggtagaggg aagaaacgct
    10381 gtcatcgagg cgttccgctc cggcaagacc gtggacaagc tgttcgtcct tgcaagcagc
    10441 caggacggcc cgatccgcac gatcatccgt gaggcgaaaa aacacgatac catcatcaat
    10501 tatgtagaaa aagaacgtct tgacgagctg tccaccaccg gaaagcatca gggtgtcatt
    10561 gcacaggtgg cttcctacag ttatgcaacc gttgaggaca tcctcgcgcg cgcagaggag
    10621 aaaggagagc cgccatttat ccttctgctg gacggcattg aggatccgca taacctcggt
    10681 gcgatcatcc gtaccgcaaa cctggcaggc gcacatggtg tcatcgtgcc gaagcaccgc
    10741 gcagtcggcc tgacagcaac cgttgccaaa acgtcggcag gtgcattaaa ttacacaccg
    10801 gtggcaaagg tgacgaatct ggtgcagacc atggaaatgc tgaaggaaaa gggcatctgg
    10861 tttgtctgcg cggacatgaa cggacagaga atgtatgatc tcgacctcaa aggaccgatc
    10921 ggccttgtaa tcggaaatga gggagagggt gtcagccgtc tggtgagaga aaactgcgac
    10981 ttcacagcca caattccgat gaaaggcgat atcgactctc tgaacgcttc cgtggcagcc
    11041 ggcgtgctcg cttatgagat tgtaagacag agaagctgct agaaattgcg gaaaatcaca
    11101 ttacgctccg gcggaagaaa gatgaaggat acaagagata tgatggtgaa agattacgat
    11161 acctgtacgg atgaggagct gattgcgcgt ctgcgggcag gagaacgcga gattaccgat
    11221 tatctgatcg acaaatacaa atcgctggtg cggaccaggg cgagggcgct gtacctggtg
    11281 ggcggagatc acgaggatct gatccaggag ggcatgctcg gactttttaa ggcagtgcgt
    11341 gactacaaac cggggaagga ggcatcgttt gccaccttcg ccggcctgtg catcgaccgt
    11401 cagatgtatt ctgcagtggc atcgtcccag cgccagaagc atcagccgct gaacagcttc
    11461 gtctcgttaa gcgaaccggt ttccgagcaa gagctgcgcc tggtggacga ggagacaccg
    11521 gaggagatca tgatcagccg ggaaaacgta atcggcatgc atgagcggat caaagaacgg
    11581 ctcagcaaat ttgagtacca ggtgctggaa ttgtatctga aagggtacga ttatacgcag
    11641 attgcagaaa aacttggaaa gcagccgaag gcaatcgaca atgcactgca gagaatccgc
    11701 agtaaagtgc gggaggtctg ttcgtgagat gggaaaacga ccgaaaaaat tacggcgaat
    11761 tgtcaaaagt gcagaaaaat ctgtgaacaa ataaaaattt tcaaatttgt agaaaaaact
    11821 gttgacaatt cactatagtt ttgatagaat accaaatgta gcaaggagct aacaaaaaaa
    11881 caaaacaact tgcgagtgct gatgtggctc agtcggtaga gcgtcgcctt ggtaaggcgg
    11941 aggtcacggg ttcgattccc gtcatcagct tactaagaaa gatcccggga aaccgggatc
    12001 ttttcgttat ataaaaaaca gcaggagatg ggcaggaaga aaaagacact attacaaaag
    12061 gtacagttat gattgtgagg gaaaaagaag atgcgtcatt cgcttggggg aaagaaagct
    12121 cgaatataag gattccaaag gacaggactg ttatttctat tattcgaaca ttgccggaac
    12181 cgatctggat atgccgttct atgtctgcaa tgccgagtgt gtcagcggca tgaaaaagag
    12241 agaatccaaa tcggagacat tcagcgcaaa tccgtttgaa tacgaattct tatacgcacc
    12301 gctcggggac aagggcgctt acgcgtacca gcagccgtgg tatggatttt cggtcaacaa
    12361 agacagcgaa gagaaagaga tcgcggttga gtttctgcgc tttatgatgc aggaaaaaga
    12421 gatcgacgag atggccagta ttaaaggcct gccgtcggtg gcagaaaacg gaaccgatga
    12481 gcggtatgcg gggcttcggg atgcaaaaaa tattgaggca agttttatca acgacggcag
    12541 cgtgccggat tccatccgcg cggcattgga gcaggcgtgt acggcaatgc aaacgaggca
    12601 gtgcaggtgt ttgtggagct gtgtgcagag aaataagata ggaaaacgag aagagcgact
    12661 catttgtgaa aacaagtggg tcgctttttg gtgccaaaga tggtagaata ggtacaacag
    12721 aaaaaatgag gtgaagaaaa caatggagtc aaaaagtaag gtactgcaaa aactgttcct
    12781 ttccacgctg tatctgagtg catttacgtt cggcggcggt tatgtgattg tgacgctgat
    12841 gaagaagaaa tttgtggatg agtacca
//
LOCUS       DAOMUF010000044        12838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1101_length_12838_cov_2.756239, whole genome shotgun sequence.
ACCESSION   DAOMUF010000044 DAOMUF010000000
VERSION     DAOMUF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1101_length_12838_cov_2.756239"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PLN77_04115"
     CDS             complement(<1..976)
                     /locus_tag="PLN77_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HRM43500.1"
                     /translation="MLQSYTEYVKRYGIAELVFRGPSEGNPFAEQWVKGTMAGQAEEK
                     HAEGFYDGNGVYKLRFMPSCEGTYEITAVTSWGDEAKVTVEVSAADENCHGPVRVANT
                     YHFAYDDGKEYYPCGTTCYVWELQSKETQEKTYESLAASPFNKIRFCVFPKHYVYNLK
                     EPAQYPFEIRENSPWSPSDFETEKLEKAPRNMFGGIDAMIENPDEVWDYTRPNPSYFE
                     HIENTIVRLGTMGIQADLILFHPYDRWGYSRMNLEQQNFYLRYVVNRFSAYHNVWWAM
                     ANEFDLFRWKPVSEWESNAETVCRQDPYRHLRSIHNCMTMYDHSRGWIT"
     gene            1275..1955
                     /locus_tag="PLN77_04120"
     CDS             1275..1955
                     /locus_tag="PLN77_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43501.1"
                     /translation="MKKRFSKISIHSLGFRIFVLLAVMIVFFCALVVYNNTAAFGLML
                     ERIHENSENTLVLYQKSLDENLSRTETYLYVFALNDADLLSLRAAEPQTTNWYITLNR
                     IKKSFENATPNYTVDGFFCYQEATDALVLYDQTTNPTPFLWNYIREIAKTEDPSSVWN
                     LNEINGKYYLVRILNLNGYLLGAYISTDTLLGTLVDTKTQDSLLYFSDGSLLLRTPSN
                     DVRLEAPA"
     gene            1952..2989
                     /locus_tag="PLN77_04125"
     CDS             1952..2989
                     /locus_tag="PLN77_04125"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM43502.1"
                     /translation="MKWRRYPSYDIDGTSWMAVSHELNETPLSLTLLLKASEYTKYQS
                     SFYLLALIVSLGLFVIWLLLSASLHRWVLHPVHMLTHALEQLRGGDLKVRIPDDAMLD
                     EFRKMTTAFNDMVEEIDDLKIANYEKQLARQKLEALYLKQQITPHFMINCLNTIYQLT
                     ENNHADLARQMLQNLSVHLRYTLSSGQTVSLLEELNLVKNYVELSSIRYPGALRLLPS
                     CEESLQNATVVPLILLNFVENTIKYEVVMGKVLDMHIDITAQEENQCTRLHICIWDTG
                     CGFSEDILAVLQKLDTYVNSEQEHIGITNVVLRLRQIFPDAAFAFGNRPGAGAQITID
                     FPYVPFFRIDG"
     gene            3025..4605
                     /locus_tag="PLN77_04130"
     CDS             3025..4605
                     /locus_tag="PLN77_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM43503.1"
                     /translation="MNLLIVDDEYYTVESISNKIRTLRPDFFEIFCAYNLTQALEAFS
                     AHPIDLMICDIEMPGGSGLELLDQIRQAHYETVCIFLTAYAKFEYISRAMKLSSSDYL
                     LKPLDDHALLEALDKACAQCEKQQKDHVNTLHANYWKESELPLAEQFFLDLLNGSISS
                     APSEVMDEIRYRRLNVELIHQPFCPLLIQTGQTNSFSPTDNRTLYEFALKNIAREYFF
                     EPGELPLTIRVLDGFYLLLLPASTHSRDAVIGRCRQALTDFVTHFPFSINFFVDQKVC
                     LPEDLRKAFLSMKAFAGKNVTYENHVFDLAAETEAPVQTRFQPIPSDRWSDLLTSGRT
                     EQLQIEVFAFLNQTKSSGTATREFLTGFYYQFLQLLFRQMDHPGSEDEAFRRQITVAS
                     PEQTFSSFGALREWISNSLLLFTQSRQAAASQDTAVATVKAYIESHLSDELDRDTLAA
                     MVYLNADYLSHLFKKDTGSSLINYIIDRRIARSKELLAKGEMSIRDIAITCGFQNISY
                     FSRQFKKSTGMTPRQFRN"
     gene            complement(4669..5568)
                     /locus_tag="PLN77_04135"
     CDS             complement(4669..5568)
                     /locus_tag="PLN77_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM43504.1"
                     /translation="MKTREDKIYSFFGHLIMTILTLLAIIPILLMFISSLTDNATLLQ
                     NGYSFLPEKWSTYAYEWVFTSSSSMVIKAYGMSFILTATGVVCSLALTTCLAYGLSKK
                     GLPGRNVLTFLVFFTMLFNGGLVPTYINYTTVFHVKDTFFGLLVPSLMMSAFNVLLMK
                     SYFVTGVPDEILEAAYIDGANEFQTMWKIAIPLSKPIITTVAMFSGIAYWNDWNNGYI
                     YLTKRTDLYSIQNLLNRLMQNIQALTQNANNMAQAGEGLSQIPSGTVRMAMAALGILP
                     IMIIYPFIQKNFVKGITLGGVKG"
     gene            complement(5579..6517)
                     /locus_tag="PLN77_04140"
     CDS             complement(5579..6517)
                     /locus_tag="PLN77_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM43505.1"
                     /translation="MARVKSQKSQKRRKTFVRFLPIYILMLPGLIYLFINNYMPLPGL
                     VIAFKQYNAGKGIYGSPWVGLKNFEYLFTTSDAFVITRNTILYNVAFIVINTTLAIAV
                     AIILSELEGKRKKVYQSAILLPNLLSTVIIGYLVFAFFSVENGFINNTILKALGKDPV
                     SWYSEPKYWPFILVFVSAWKSVGYNCIVYLATLMGFDKSFYESAKIDGATRWQQIKYI
                     TLPLLRPTIIMLTMMAIGRIFYSDFGLFYQVPMNQGALYSTTNTIDTYVYRGLLQMGN
                     VSMSAAAGVYQSIVGFILVLGANLLVRKIDRDSALI"
     gene            complement(6654..8204)
                     /locus_tag="PLN77_04145"
     CDS             complement(6654..8204)
                     /locus_tag="PLN77_04145"
                     /inference="COORDINATES: protein motif:HMM:NF023435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43506.1"
                     /translation="MKKKVAAIFMASIMACSMAACGSSGGSSDSGKTDSKDKSTASST
                     AENGSGEVEKIIVSFPTWTGAPADTEKVQEAINDITRDKIGVEIELSITDFGSFNQNT
                     TLALSANEEIDVLACVGFPYATGIQQGYLSDLEENDLLATYGPDIAEAVGQDNIDACR
                     VNGVLYGLPSNRDIAQGRGCAAIATEYLDGIGYEANTDDEIVKIGLDELNDIYAQLHE
                     KYPDKEVYRPTTGSMSQFSNVDSLGGNVFGVLLDYGQNLDVVNLFESDFYKDYCARLY
                     DYNQKGYISADASTDTTAVGELVKAGTLMSYTTGGKPGIKAQETTLTGRDMTIFQTLD
                     DYISSTSVASMPWTIPISAQHKEAAMKFLNECYTNADIANLLAWGIEGTHYKIGDDGL
                     ATYADGVDASNSGWNHSMGWMMPNQFLTHVWEGNDPDLWTEMKEFNTNAKVSKASGFS
                     FDSTNVANELTAVQNVYNEYQTSVEYGFVDPETGIAEMNDKMMTAGLQKIIDEKKAQL
                     AAWQEANK"
     gene            complement(8380..9993)
                     /locus_tag="PLN77_04150"
     CDS             complement(8380..9993)
                     /locus_tag="PLN77_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HRM43507.1"
                     /translation="MGMKAEQWRQIILSFESKKTYDNPFLDVSIWSIFTGPSGRRIRR
                     EAYWDGGNTYRIAFAPTETGMWSYRLEAPEETGLSGVQGTIECIPYEGNLAIYQHGFL
                     KVHPSGRYLTYADGTPFFWLGDTHWAFAFGERWDESNHPQMTSMFRGMADRRKEQGYN
                     VYQTNLRSDSWKEHKSRYWKTDATDDVPDVAFYQNELDRRMQYLADLGFINALGFAWS
                     GSIEQGVEHQKHLARYIIARYGALPVVWTLSGEVAGYFPGKPRETAIKGWREVAKYVE
                     KMDGYGTLQTAHYTNERPFADYYYDESWFDFVLNQAGHGDFPINPSWYRAYRKEHGTK
                     PFIEGESLYEYCSTLEENGTRLCTDAMLRRVAYMAVQTGGCGYTYGAQGIWDNIWEVS
                     DINPDFNAFNKFGITWAKAIDGPGGAQMGYLKRFYEEHHFEEMVPYEPAEMEESISPF
                     ANKLAAATISRDKTRALIYYGEMDGMSTPLAGLCDGIYAISWFDPRTGQTEKAEIQVE
                     VRGKSCIMPKKPAAGDWVLIAECIEKTEK"
     gene            complement(10091..11554)
                     /locus_tag="PLN77_04155"
     CDS             complement(10091..11554)
                     /locus_tag="PLN77_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HRM43508.1"
                     /translation="MITRLEEIPVTKESYPFASAERYLKLSERGYVEKEYYMYGTANV
                     YETADERGGVRVRTADAPYTNRIIVRAPQDTAKCSGNVVVEIINPTSFMEIDRMWILG
                     WKKFVRDGDIYVGITSKPNTIAKMVEFDEKRYGRLSWANPTPDVPFSERLQVTGGLGD
                     LNHDYETGLFWDMLTDLAWLLRGDEEQNPIREYKREYVYLTGWSQSGSYLFRYLNSFA
                     YRPEVARGACVFDGYLSGGGVHSLGIPVNQYETMTPYHYSLTRVEKAREPFIAVQTES
                     ENGRMEAWRIAQQDSDDPEFLFRQYDVTGASHDTSYSYVNYYQNDPDLVRINHLPFYM
                     GKNEEGNDYPSQILFAAAFRNLFTWVRTGAAPAHCDRIAVDSDGENKKDAFGNTIGGL
                     RTCLLDYPTGRYSNTSDIEKGQNFLDPESEKEGLFGYQQAFSKEMIKELYGTLAHYRE
                     MAEKNTKEQVTKGFVCREDAAELVEIAVQLAKKRGLE"
     gene            11943..>12838
                     /locus_tag="PLN77_04160"
     CDS             11943..>12838
                     /locus_tag="PLN77_04160"
                     /inference="COORDINATES: protein motif:HMM:NF036873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43509.1"
                     /translation="MNGTYYTITEVFDFGPHISKVILDYGKSMKGAVPSPEQFTVHVT
                     RTSTEGEDFVWPNFMGDKPDDSMDGTRRVSNVYVSDKTGAPCEDGTCLTLELPCFIME
                     GIGSIIKFNGNFNVFVKVAYDVTQVSEIATDDGAISPQMFDVDGGNRVIYGEWLKEDR
                     YEDPQIPLSYVYYEPEMDADEKIPLIIWLHGAGEGGQEPPIAAIGNKVVNLISPKVQK
                     IFGGKTYLLAPQAPTMWMDDGSGEYTKDGSSKYTEVLDALIGAFVDAHPQIDRSRIYI
                     GGCSNGGFMTMKQIIFNPSRYAA"
BASE COUNT         3039 a         3388 c         2806 g         3605 t
ORIGIN      
        1 gcgtgatcca tcccctggaa tgatcgtaca tggtcataca gttgtgaatg gagcgcagat
       61 ggcggtacgg atcctgacgg cagacggttt ctgcattgga ctcccactcg gataccggtt
      121 tccagcggaa caggtcaaat tcatttgcca tagcccacca tacattgtgg tatgcgctga
      181 aacggttaac aacgtatctt aaataaaagt tctgctgctc cagattcatg cgtgaatagc
      241 cccagcggtc gtacggatgg aacaaaatca gatcagcctg gattcccatg gtgccaaggc
      301 gtacgatcgt attttctatg tgttcaaaat aagaaggatt tggacgggta taatcccata
      361 cttcatccgg gttttcgatc atggcatcaa tgccgccgaa catattgcgc ggcgcttttt
      421 ccagcttctc tgtctcaaag tcagacggag accacggaga attttcacga atttcaaacg
      481 gatactgtgc cggctctttt aaattgtaga catagtgctt cgggaaaacg cagaagcgga
      541 tcttattaaa cggagaagca gcaaggcttt catacgtttt ctcctgagtc tccttactct
      601 gcagctccca cacatagcag gtcgtaccgc acgggtagta ttcctttccg tcatcgtaag
      661 caaaatgata ggtgtttgct acccggaccg gaccgtggca gttttcatcg gctgcgctga
      721 cctccaccgt cacctttgcc tcatcgcccc agcttgttac tgcggtaatc tcatacgttc
      781 cttcgcagga tggcataaag cgcagtttgt atacgccgtt tccatcataa aagccctcgg
      841 catgcttttc ctctgcctga cctgccatcg ttcctttcac ccactgctcc gcaaacggat
      901 ttccctcgga cggtccccgg aaaaccagtt ctgcaattcc atatcgtttt acatattcgg
      961 tataagactg taacatacgt tccacctttc taaaataagt attgcttcgt atcgcgataa
     1021 tttaatttta ctaaatgtat tttgaatagt caatcttttt aaatttaatt tttaaacaat
     1081 aattcagtta tttttttatt acttttgccg gtatccgttt cttttttcct tgtatttatt
     1141 gacgtttttt cacttttatt gtaaactttc ccatagagtc tgccggacct tttgctgaac
     1201 agttccggca ttttcttttt atctggctca aaattcagca tatatctaac atgtacagga
     1261 gaggagatgg tcctttgaaa aaacgctttt ccaaaatttc catacactca cttggttttc
     1321 gtatttttgt tctgcttgca gtcatgatcg tctttttttg cgcgcttgtc gtgtacaaca
     1381 acacagccgc tttcggtctg atgcttgagc ggatccatga gaactcggaa aatacactgg
     1441 ttctgtacca gaaaagcctg gatgaaaatc tttcccgcac ggaaacctac ctctatgttt
     1501 tcgcattaaa tgatgccgat cttctttccc tccgcgcagc agaaccacag acaaccaact
     1561 ggtatatcac attaaaccga atcaaaaaaa gctttgaaaa cgcaacgcca aactataccg
     1621 tagacggctt tttctgctat caggaagcca cagatgccct ggtcctctat gaccagacca
     1681 ccaacccgac accgttcctc tggaactata tccgtgaaat cgccaaaaca gaggatccgt
     1741 cctctgtatg gaatctcaac gaaatcaacg ggaaatatta ccttgtccgt attctgaacc
     1801 tgaacggata tctgctcggc gcgtacattt ccacggatac actccttggc actctggtcg
     1861 atacaaaaac gcaggacagc cttctgtatt tttccgatgg ttctcttctt ctgcgcaccc
     1921 cgtcaaacga tgtccgcctg gaggcccccg cttgaaatgg cgacgatatc cttcctacga
     1981 cattgatgga acctcctgga tggctgtttc acatgaactg aacgaaacac cgctgtcgct
     2041 gaccctgctt ttaaaggctt cggaatacac caaatatcag agcagctttt atcttctcgc
     2101 cctgatcgtt tcgctcggtc tgttcgtaat ctggctgctc ctgtctgctt ccctgcaccg
     2161 ctgggttctt cacccggttc acatgctgac gcatgcgctg gaacagctcc gcggcggtga
     2221 tctcaaggtg cggattccgg atgacgctat gctcgacgaa ttccggaaaa tgacgaccgc
     2281 tttcaacgat atggtcgaag agatcgatga tctgaaaatc gcgaattacg aaaaacagct
     2341 cgcccgtcag aagctggaag cgctttacct gaagcagcag atcacgccgc attttatgat
     2401 caactgcctg aacaccattt atcagctgac tgaaaataac catgcggacc tggcacggca
     2461 gatgcttcag aacctgagtg tacatctgcg ctatacgctt tcctccggcc agaccgtctc
     2521 gcttttagaa gagctaaacc ttgtaaaaaa ctacgtggag ctttcctcca tccgctatcc
     2581 cggcgcgctg cgcctgctcc cttcctgcga agaatcgctg caaaatgcaa cggttgtgcc
     2641 gcttatactc ctgaactttg tggaaaacac gatcaaatat gaggtcgtta tggggaaagt
     2701 gctggacatg catatcgata tcaccgcgca ggaagaaaat cagtgtaccc gtcttcatat
     2761 ctgtatctgg gataccggat gcggtttttc cgaggatata cttgccgtgc tgcaaaagct
     2821 tgatacctat gtaaactccg agcaggaaca catcggcatc acaaacgtgg tgctccgcct
     2881 ccgccagatt tttcccgacg cagcttttgc ttttggcaac cgccccggcg caggcgctca
     2941 gattaccatt gattttcctt acgttccctt tttccgcatc gacggataac cggaacagct
     3001 aaaaatatgt tttgggaggt tattatgaac ctgttaattg tagacgatga atattatacc
     3061 gtcgaaagca tttccaacaa aatccgcaca ctacgcccgg acttttttga gattttctgt
     3121 gcctacaatc tgacacaggc attggaagcc ttttccgcgc atccgattga tctgatgatt
     3181 tgtgatatcg agatgcccgg cggttccggc ctcgaactgc tggaccagat ccgccaggca
     3241 cactacgaaa ccgtctgcat ttttctgacc gcctatgcaa aatttgaata tatttcccgc
     3301 gctatgaagc tttccagcag tgactatctg ttgaagccgc tggacgacca tgctcttctg
     3361 gaagctcttg acaaagcctg tgcgcaatgt gaaaagcagc agaaggacca cgtcaacacc
     3421 cttcatgcca attactggaa ggaaagtgag ctcccgcttg cagagcagtt cttcctcgac
     3481 ctactaaatg gcagtatttc ctctgctcct tccgaagtga tggatgaaat ccgctatcgc
     3541 cgtctgaatg tcgaactgat tcatcagccc ttctgtccgc ttctgatcca gaccggacag
     3601 acgaactcct tttctccgac cgacaaccgt accctgtatg aatttgcctt aaaaaatatt
     3661 gcccgggaat atttttttga acccggcgag ctgccgctga cgatccgtgt gcttgatggt
     3721 ttttatcttc tgcttctccc ggcctccaca cactctcggg acgccgtgat tggccgatgc
     3781 cgtcaggccc tgaccgactt tgtgacacac tttccgttct cgatcaactt tttcgttgat
     3841 cagaaggtct gtctcccgga agacctgcga aaagcttttc tttccatgaa agccttcgcc
     3901 ggaaagaacg tcacctacga aaatcacgtt ttcgatcttg ccgctgaaac cgaagctcct
     3961 gttcagacgc gttttcagcc gattccatct gaccgctggt ccgatcttct cacatccgga
     4021 agaacggaac agcttcagat tgaggttttc gctttcttaa atcagacgaa aagctccggc
     4081 actgctacac gggaatttct gaccggcttt tactatcagt tcctgcagct gctgtttcgc
     4141 cagatggatc atccgggcag cgaagacgaa gccttccgcc gccagattac cgttgcttcc
     4201 ccggagcaga ccttctcctc gtttggtgct ttacgagaat ggatttccaa cagtcttctg
     4261 ctgttcacac aaagccgtca ggcagccgct tcccaggaca cggctgttgc caccgtgaag
     4321 gcatatatcg aatcgcacct ctccgacgag ctggatcgcg atacgcttgc ggccatggtg
     4381 tatctgaacg ccgattatct gtcccatctc ttcaaaaaag acacgggaag ttctctcatc
     4441 aactacatca tcgaccgccg gattgctcgc tccaaggagc ttctcgcaaa gggcgaaatg
     4501 agcatccgcg acattgccat cacctgcggc ttccagaata tttcgtactt ctcccgccag
     4561 tttaaaaaat cgacgggcat gacaccacgg cagtttagaa attaaaaaaa tccggccttc
     4621 cttttgcata tcggaaagcc ggattttttg gattcactta cttttttatc agcccttcac
     4681 gccgccaagg gtaatacctt ttacaaagtt tttctggatg aacggataaa tgatcatgat
     4741 cggcagaata ccaagtgctg ccatcgccat acgaaccgtt ccggatggga tctgggagag
     4801 gccttcgcct gcctgcgcca tgttgttggc gttctgcgtc agcgcctgaa tattctgcat
     4861 cagacggttc agcaggttct ggatactgta gagatccgtt ctctttgtca gatagatata
     4921 accattgttc cagtcattcc agtaagcgat accggagaac atggcaacgg tggtaataat
     4981 cggtttggac agcggaatgg cgatcttcca catagtctgg aattcgtttg caccgtcaat
     5041 ataggcagcc tcaagaattt catccggcac accggtgaca aagtagctct tcatcaggag
     5101 aacgttgaat gcgctcatca tcagagacgg aaccagcagc ccgaagaacg tatctttcac
     5161 atggaatacg gtcgtgtaat tgatataagt cggtacaagg cctccgttga acagcatggt
     5221 aaagaacacc agaaaggtca ggacattgcg tcccggcaga ccttttttcg aaagaccata
     5281 ggccagacag gttgtcaggg caaggctgca gacaacgccg gttgcggtca ggataaagga
     5341 cattccgtag gctttgatta ccatggaact gctggatgtg aatacccact cataagcata
     5401 ggtactccac ttttccggca gaaaagagta tccattctgt aacagagttg cattgtcagt
     5461 caatgaggaa atgaacatca acagaatcgg aatgatcgcg agcagtgtca ggattgtcat
     5521 gatcagatgg ccgaaaaagc tataaatttt atcttctctt gttttcatac cgttcctcct
     5581 agatcagcgc gctgtcgcgg tcaattttac gtaccagcag gttggcgcca agcaccagaa
     5641 taaatcctac gatcgactgg taaacaccgg ctgcggcaga catcgaaaca ttgcccatct
     5701 gaagcagtcc gcgatatacg taggtatcaa ttgtgtttgt tgtggaatac agagctccct
     5761 ggttcatcgg cacctggtaa aagagaccga agtcagagta gaaaattctt ccgattgcca
     5821 tcatggtcag catgataatg gtcggacgca gaagcggcag ggtaatatat ttgatctgct
     5881 gccatctcgt tgcaccatcg atcttggctg actcatagaa ggatttatca aatcccatca
     5941 gtgttgccag atacacaata cagttgtagc caaccgactt ccatgcactg acaaaaacca
     6001 ggataaacgg ccagtatttc ggttccgaat accaggaaac cggatctttt cccagcgcct
     6061 ttaaaatcgt gttgttaatg aatccgtttt ctacactgaa gaatgcaaaa acaagatatc
     6121 cgatgataac ggtggagagg aggttcggaa gaaggatggc gctctggtaa acttttttcc
     6181 gttttccttc cagctcactt aagataatag ctactgcgat tgccagtgtt gtgttgatta
     6241 cgataaatgc cacattgtag agaatggtat ttcgtgtaat aacaaaggca tcgcttgtcg
     6301 taaacagata ttcaaagttt ttcagtccta cccatggact tccgtagatg ccttttccgg
     6361 cattgtactg tttgaatgca atcacaagac ccggcagagg catgtaattg ttgataaaca
     6421 gataaatgag acccggcagc atcagaatat agatcggcag aaaacgtaca aatgtttttc
     6481 gtcttttctg gctcttctga cttttcactc ttgccatgtt tttccttctc cttttttctt
     6541 atccgcagcc ggtcccaaag cgttccggtt ttgacagatt tttctcttct tacaacctgc
     6601 gccgtcctgt ttttgggacg gcgcagtcat tttttgatct aaattcttaa actttatttg
     6661 tttgcttcct gccacgctgc aagctgtgcc tttttctcgt cgataatctt ctggaggcct
     6721 gcagtcatca tcttatcatt catttcggca attccggtct ccgggtctac aaagccgtac
     6781 tctacgctgg tctgatactc gttgtatacg ttctgtactg ctgtgagctc gtttgccaca
     6841 tttgtgctgt caaaggagaa accgcttgct ttggaaacct ttgcattggt gttaaactct
     6901 ttcatctctg tccacagatc cggatcattt ccttcccata catgggtcag gaactggttc
     6961 ggcatcatcc atcccatgga atgattccat ccggagttgg atgcatctac accgtctgca
     7021 taagtagcaa gtccgtcatc gccaatttta taatgtgttc cctcgatgcc ccatgccaga
     7081 aggtttgcga tatcggcatt tgtgtagcac tcattcagga atttcatggc tgcttcttta
     7141 tgctgtgcag agatcggaat ggtccaaggc atactagcca cagatgtgga actgatatag
     7201 tcatccagtg tctggaagat ggtcatatcg cgaccggtca gtgtagtctc ctgcgctttg
     7261 atgccaggct ttccgcctgt ggtatagctc atcagagttc cggcttttac aagctctccg
     7321 actgctgttg tatcggtcga tgcatcggca ctgatatagc ctttctgatt gtagtcatac
     7381 agtcttgcac aataatcctt gtagaaatcg ctctcaaaga gatttacaac atcaaggttc
     7441 tggccatagt caagcagaac accgaacaca tttcctccaa gggagtctac gttagagaac
     7501 tgcgacatag aacctgttgt cggacgataa acctctttgt ccggatattt ctcatgcagc
     7561 tgtgcataaa tatcgtttaa ttcatccaga ccgattttta cgatctcatc gtctgtgttt
     7621 gcttcgtagc caataccatc gagatactcg gttgcgatcg ctgcgcatcc acgtccctgt
     7681 gcaatgtccc ggttgcttgg aaggccatag agaactccgt tcacacggca ggcatcaatg
     7741 ttatcctgtc caactgcttc tgcgatgtcc ggtccatacg ttgcaagaag atcgttctct
     7801 tccagatcgg aaagatatcc ctgctgaatt ccggttgcat atgggaaacc tacacatgca
     7861 agtacatcaa tttcctcatt tgcggaaagc gccagtgtcg tattctgatt aaaggatccg
     7921 aaatcggtaa tagaaagctc aatttcaacg ccaatcttat ctcttgtgat atcattgatg
     7981 gcttcctgta ctttctccgt atctgccgga gcacccgtcc aggtcgggaa gcttacgatg
     8041 attttttcca cttctccgct tccgttctct gctgtactgg atgcggtact cttatcctta
     8101 gaatctgtct ttccggaatc tgaacttccg ccgctgcttc cgcatgcagc catggagcat
     8161 gccataatgg atgccatgaa aatcgctgct actttctttt tcattctttc gtcctcgctt
     8221 tttatctttt tgtcttgctg ctttatttcg tttctgagtt tcggccgtga ctcatttgat
     8281 gatcttatta tatctgcttt cttacctcca aaaaagaaca atcctgcgat gaatttgcac
     8341 ttttctgcgg tttctttccg cattttgtat agtttatgat cacttttcag tcttttctat
     8401 gcattctgct atcagcaccc aatctcccgc cgccggtttc tttggcataa tgcacgactt
     8461 tccacgcact tccacctgga tttctgcttt ttctgtctgt cccgttctcg gatcaaacca
     8521 ggaaattgca tagattccgt cgcaaaggcc cgcgagcggt gtactcatgc catccatctc
     8581 tccgtaataa atcagtgctc tcgttttatc ccggctgatc gtggcagccg caagcttatt
     8641 ggcaaacggg ctgatactct cttccatttc tgccggttca tatggcacca tctcctcaaa
     8701 atgatgttct tcatagaaac gtttcagata tcccatctgt gcgcctccgg ggccgtcaat
     8761 ggctttcgcc caggtgatgc caaatttgtt gaatgcatta aaatccgggt tgatgtcact
     8821 tacctcccag atattatccc agattccctg cgcaccgtac gtatagccgc agccgccggt
     8881 ctggactgcc atatatgcca cgcggcgcag cattgcatcg gtgcagagcc gggttccgtt
     8941 ttcttccagt gtggaacagt attcatacag ggactcgcct tcgatgaatg gttttgttcc
     9001 gtgttccttc cgataggcac ggtaccagga cggattgatc gggaaatcgc cgtgtccggc
     9061 ctgattcagc acaaaatcaa accacgattc gtcataatag taatctgcaa acggacgctc
     9121 attcgtataa tgtgcggtct gcagtgttcc atatccgtcc attttttcca catactttgc
     9181 gacctcgcgc cagcctttga tggctgtttc ccgcggcttt cccgggaagt agcctgccac
     9241 ttcgcctgac agcgtccaca caaccggaag ggcaccatag cgggcaatga tatagcgtgc
     9301 cagatgtttc tggtgctcca cgccctgctc gatcgagccg gaccaggcaa aaccgagcgc
     9361 gttgataaag ccgaggtcag cgagatactg catgcggcgg tcgagttcgt tctggtaaaa
     9421 tgccacatct ggcacatcat ctgtggcatc cgttttccag taacggctct tgtgctcctt
     9481 ccagctgtcg cttcgaaggt tcgtctgata aacgttataa ccctgttcct tcctgcggtc
     9541 cgccatgccg cggaacatgc tcgtcatctg cggatggttc gactcgtccc accgctcacc
     9601 aaaggcaaat gcccagtgcg tatcaccgag ccagaaaaac ggtgttccgt ctgcatacgt
     9661 cagatatctt cctgatggat gcactttcag aaatccgtgc tgatagatag caagatttcc
     9721 ttcgtatgga atacattcga tcgttccctg cactccggaa agtcccgtct cctccggtgc
     9781 ttccaaccta taactccaca ttccggtttc ggtcggcgca aaggcaatcc gataggtatt
     9841 tccgccgtcc cagtatgctt ctcttctgat ccttcttccc gacggtcccg taaaaatact
     9901 ccaaatggaa acatcgagga atggattgtc gtatgttttc ttgctctcaa atgataaaat
     9961 aatttgtcgc cactgttctg ctttcatccc cattttattt tctcctttat ctgcgcagag
    10021 cgcgcccggt ttttttcggg cacgctcctc gaatctgatt ttaccgtaac tgtttattgt
    10081 ttattgtttt ttattccagt ccgcgtttct ttgcaagctg cacggcaatc tcaaccagct
    10141 ccgccgcatc ctcgcggcag acaaagcctt tcgttacctg ctctttcgtg tttttctccg
    10201 ccatttcccg gtaatgtgcc aatgttccgt acagctcttt tatcatttct ttcgaaaacg
    10261 cctgctgata accgaacaat ccttcctttt ccgattccgg atccaggaaa ttctggcctt
    10321 tttcaatgtc gctggtattg ctgtatcttc cggttgggta gtcaaggagg caggtacgca
    10381 gtccaccgat cgtatttccg aacgcatcct ttttgttttc tccgtcgctg tcaaccgcaa
    10441 tccggtcaca gtgcgccgga gctgcgcctg tccgtaccca ggtaaagaga ttccgaaatg
    10501 ctgccgcaaa aagaatctgg gacggatagt cgtttccttc ctcatttttt cccatataga
    10561 atggcagatg gttgatccgc accagatccg gatcgttctg atagtaattc acgtagctat
    10621 aggaagtgtc atggcttgca cccgtcacat catactggcg gaacaggaac tccggatcat
    10681 cgctgtcctg ctgggcaatg cgccatgctt ccattcttcc gttttcactc tccgtctgta
    10741 cagcgataaa cggctcgcgc gccttttcca cacgtgtcag actgtaatgg tacggagtca
    10801 tggtttcata ctggttgacc ggaataccaa gggaatggac tcctcctccg gaaaggtagc
    10861 catcaaatac acacgcaccg cgtgccacct ccggacgata cgcaaagctg ttcagatagc
    10921 ggaacagata cgaacccgac tgtgaccagc ccgtcaaata gacgtattct ctcttatatt
    10981 cccgaatcgg attctgctcc tcatcgccgc gcagcagcca cgccaggtct gtcagcatat
    11041 cccagaaaag acccgtttcg taatcatgat tcagatcgcc gagtccgcct gtgacctgca
    11101 gtcgctccga gaacggcaca tccggcgtcg ggttggccca ggaaagacgg ccgtaacgct
    11161 tttcatcaaa ctctaccatt ttagcgatcg tattcggttt gcttgtgatt cccacataga
    11221 tatctccgtc ccgcacaaat ttcttccagc cgagaatcca catgcggtca atctccataa
    11281 aactcgttgg attgatgatc tccactacaa cgtttccgct gcattttgca gtgtcctgcg
    11341 gtgcgcggac aatgatgcgg tttgtatacg gcgcatccgc agtgcgcaca cggacgccgc
    11401 cgcgctcgtc ggctgtctcg tagacatttg ccgttccata catataatat tccttctcca
    11461 catagccgcg ctccgacagc ttcaggtaac gctccgcact ggcaaacgga tagctttctt
    11521 tcgttaccgg aatctcctct agtcttgtaa tcatctcgta aaacctccct ttttccttgt
    11581 atgattttat tattccatac ggctccaata cgaaaaacaa cgattctgct gttttttgac
    11641 acttttctgc tgtttctgtt tcgatcagac tttttccgtt ccggaaacaa aaacacagaa
    11701 aaagcatctc cgtgcaaatt tatctctgat ttgtactttt tctttcggaa tgtctgtagt
    11761 agactggtat tgtagggcag aaaaaatcta tcattcccaa taccgtcttt tctgcccatg
    11821 caaacaactg tactctcaag ctttttctgt acctgatttt gtgagatgat ttttgtcagt
    11881 cgcaaaagca agtgcagaaa gactctgagc gtacattccg taccaaagga gggtttttac
    11941 aaatgaacgg aacttattat accattaccg aagtttttga tttcggtccg catatttcga
    12001 aagtaattct ggattatggc aaaagcatga agggtgcggt cccatctccg gagcagttta
    12061 cggtacacgt tacccgcact tccaccgagg gtgaggactt tgtctggccg aattttatgg
    12121 gcgacaaacc ggatgactcg atggacggaa cccgccgcgt ttcgaacgtt tacgtttctg
    12181 acaaaaccgg tgcaccgtgc gaggacggca cctgcctgac gctggagctg ccgtgtttta
    12241 tcatggaagg aatcggctcg atcatcaaat tcaatggtaa ttttaatgta tttgtcaaag
    12301 tcgcttacga tgtgacacaa gtctctgaaa ttgcaacaga cgatggtgcc atttctccgc
    12361 agatgttcga cgtagacggc ggaaaccgcg tcatctacgg tgaatggctc aaagaagacc
    12421 gctacgagga tccgcagatt ccgctctctt acgtttacta cgagccggaa atggatgcgg
    12481 atgagaaaat cccgctgatc atctggctgc acggcgcagg tgagggcgga caggaaccgc
    12541 cgattgcagc aatcggaaat aaagttgtaa acctaatttc cccaaaggtt cagaaaattt
    12601 tcggcggcaa gacctacctg ctcgctccac aggcgccaac catgtggatg gatgacggaa
    12661 gcggagaata taccaaagac ggttcttcca aatataccga agttctcgat gccctgatcg
    12721 gtgctttcgt ggatgcccat ccgcagatcg accgctcccg catttacatc ggcggctgct
    12781 ccaacggcgg ctttatgacc atgaaacaga tcattttcaa cccgtcccgc tacgctgc
//
LOCUS       DAOMUF010000045        12521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1140_length_12521_cov_3.819830, whole genome shotgun sequence.
ACCESSION   DAOMUF010000045 DAOMUF010000000
VERSION     DAOMUF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 12521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12521
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1140_length_12521_cov_3.819830"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="cas9"
                     /locus_tag="PLN77_04165"
     CDS             complement(<1..596)
                     /gene="cas9"
                     /locus_tag="PLN77_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426118.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HRM43510.1"
                     /translation="MKGEYYLGLDMGSSSVGWAVTNEKYEILRAHGKTLWGVRLFDSA
                     QTAEERRGFRTAKRRLDRRNWRIELLQKIFAEEVEKIDDGFFRRMKESRYWPEDKRDR
                     QGKCPELPYALFADDDYTDKEYHKQFPTIYHLRKWLMETDETPDIRLIYLALHHMMKH
                     RGHFLLNGNIEQVREFQTTFEQFLEAVRAEELGFSSEIG"
     gene            1010..1092
                     /locus_tag="PLN77_04170"
     tRNA            1010..1092
                     /locus_tag="PLN77_04170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1044..1046,aa:Leu,seq:caa)
     gene            complement(1249..2526)
                     /locus_tag="PLN77_04175"
     CDS             complement(1249..2526)
                     /locus_tag="PLN77_04175"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRM43511.1"
                     /translation="MTKKPFVTKEKLEEIAAAVPTPFHIYDEKGIRENAKAVKEAFSW
                     NPGFREYFAVKANPNPTLIKMLGEYDCGCDCSSYTELMLSDAMGFEKDKIMFSSNVTP
                     AAEYQLAAELGAIINLDDITHIDFLEKTLGYIPKKICCRYNPGGLFKISTDIMDNPGD
                     AKYGMTTEQIFEAFRILKAKGAEEFGIHSFLASNTVTNDYYPVLAKTLFELAVKLQKE
                     TGVKIKFINLSGGVGIPYKPDQEGNDIRVIGEGVHKVYDEVLVPAGMGDVAIYTEMGR
                     FMMGPYGGLVTRAIHQKHIYKEYIGCDACAVNLMRPAMYGAYHHITVMGKEDQPCDHK
                     YDVTGSLCENNDKFAIDRMLPEVEVGDLLFIHDTGAHGFSMGYNYNGKLKSAEVLLKE
                     DGSFEVIRRAETPKDYFATLDGTPEYEKMFGNK"
     gene            complement(2550..3986)
                     /gene="pyk"
                     /locus_tag="PLN77_04180"
     CDS             complement(2550..3986)
                     /gene="pyk"
                     /locus_tag="PLN77_04180"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRM43512.1"
                     /translation="MRRTKIVCTMGPNTNSRDLMKKLIETGMDVARFNFSHGDHDEQK
                     MRMDMLKELRKELKTPVAILLDTKGPEIRTGVLKDGKKVNLVTGQTFTLTTVQETGDE
                     NHCSVSYTGLTEDIKEGDTILIDDGLIGLRVEKVNAPEIVCTVVNGGELGEKKGVNVP
                     NVSINLPNLTEKDKGDLLFGIEQDIDFVAASFIRNAEAINEIREFLVANGGEHIDIIA
                     KIENAEGVQNIDSIIDAADGVMVARGDLGVEIPACQVPHVQKIIIEKCNHKYKPVITA
                     TQMLDSMIRNPRPTRAEVADVANAIYDGTDAIMLSGETAAGKYPIEAVTMMGEIAEQT
                     ERYLKFEDYRDGKALEGKLNVSAAVGSAAVSMVEHIKAACIVTPTMSGQTARLISNLR
                     PSVPIYGVTPYEWARRKMQLYWGVRPVTGYEEDSTENIISHAMYMVRREELVERGDMV
                     IFTAGDPATNEVTGEGYMTNMLHIIQAK"
     gene            complement(4002..4865)
                     /locus_tag="PLN77_04185"
     CDS             complement(4002..4865)
                     /locus_tag="PLN77_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43513.1"
                     /translation="MNFLDKMERKYGRYALSHLTMYIIVTYIAGYIIALAAPIMRQYL
                     TLEPYYILHGQIWRLVSWILIPPSSLDIFTIIMLFFYYSIGTSLERAWGDFKYNVYIF
                     SGILMTIIGSFLLYGILYAVNGYPSLMGTAFSTYYISLSIFLGFAISFPDMQVLLYFI
                     IPIKIKWLAYLDVALLAYSMITSIMSGNWAGCVVILCSLANVLVFFLMTRKGKRGSFQ
                     QNRRRKEFKKAVSRGEAEYRNPNGITKHKCAICGRTEKDDPNLEFRFCSRCNGNYEYC
                     QDHLFTHEHVK"
     gene            5084..6190
                     /gene="aroC"
                     /locus_tag="PLN77_04190"
     CDS             5084..6190
                     /gene="aroC"
                     /locus_tag="PLN77_04190"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRM43514.1"
                     /translation="MAGSSFGTIFRLTTWGESHGKGVGVVVDGVPAGLSLSEEDIQVY
                     LDRRKPGQNRFTTKRKESDTVQILSGTFEGKTTGTPISMVVWNEDQHSKDYSDIASYY
                     RPGHADYTFDAKYGFRDYRGGGRSSGRETIARVAAGAIALKMLSELGITVSAYTRSIG
                     DVEIQSFDAAEIANNALNMPDAEAAEKASELLTKAMAEKNSVGGVVECVVHHMPAGVG
                     DPVFEKLDANLAKALVSIGAVKGVEIGDGFSVCTATGLTNNDAFHVNADGSIVKLTNH
                     AGGILGGISDGSDIVVRAGFKPTPSVAASQQTINKDGENIAIEIKGRHDPIIVARAVV
                     VVECMTALVLADALLVGMSAKLDNVKKVWALDEK"
     gene            complement(6275..7333)
                     /locus_tag="PLN77_04195"
     CDS             complement(6275..7333)
                     /locus_tag="PLN77_04195"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HRM43515.1"
                     /translation="MKDEYVAYVGTYTHGSSIGIHLYDVNVEEGTLTERKVIPVNNSS
                     HIARSLNGKYLYSIADEGVAVFAVEDDGDLTPINKVDIDGMRGCYLSVDKTGRYLFVA
                     GYHDGKVTVVHTHKDGRLGSLMDGVFHKGLGSVAERNFRPHVSCVVPTPDNKYVCAVD
                     NGIDQIKMYKLNTQRDRLKLVDILRCERESGPREIVFSESGKYAYVIYELMNKVDVFS
                     YRDTGNGPEFEKLQSISTTSANVDQAHDAASGMCLSPDHHYLFTSTAGDNTVAMFHID
                     PETGCMEKKFALPISGEYPKDIAMFPDGKHIAVANHESNTITTFTVDYEKNVLVQKGR
                     PMKVETPNCILITKKGVH"
     gene            complement(7557..8084)
                     /locus_tag="PLN77_04200"
     CDS             complement(7557..8084)
                     /locus_tag="PLN77_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM43516.1"
                     /translation="MRQEEERFFQIIFEENSHVLFRYICGQLNNDRETAKDVLNDVWE
                     VLVVKFETVADLEGEEMTKWLFCVAKNKIKNQRRKHYYQNEVSYTQYQKENRNEGEAA
                     ATAEIEERMVMQNCIQNLNPMDQRIFWGRAYGFSYKELSGFCKKSEQALQCQYSRAIK
                     KLQGMMSQEISLTRA"
     gene            complement(8363..12160)
                     /locus_tag="PLN77_04205"
     CDS             complement(8363..12160)
                     /locus_tag="PLN77_04205"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRM43517.1"
                     /translation="MSTVRRVYVEKKPEFAIKAKDLKHEIKHYLNIQTVTNVRELIRY
                     DVENISDETYEKACKVVFAEPPVDICYQEDFPRAEGARVFSVEFLPGQFDQRADSAVQ
                     CVQFLKEDEQPIIRSAVTYVIEGEMTDEQFDAVKAHCINPVDSRETGLEKPETLVTKF
                     EEPADVKIFDGFIDMPEAELKALYDSLGLAMTFKDFLHIQNYFRGEEKRNPSMTEIRV
                     LDTYWSDHCRHTTFSTELTNVTFGEGDYYEPMKKSYDRYLSDREVLYKGRDDKFICLM
                     DLALLAMKKLKSEGKLADQEESDEINACSIVVPVDVDGKDEEWLINFKNETHNHPTEI
                     EPFGGAATCLGGAIRDPLSGRTYVYQAMRVTGAADPTRPQSETLEGKLPQKKLVREAA
                     HGYSSYGNQIGLATGYVKEVYHPDYVAKRMEIGAVLGAAPRRAVVRENSDPGDIIILL
                     GGRTGRDGCGGATGSSKVHTTESIETCGAEVQKGNAPTERKLQRLFRREEVSLLIKKC
                     NDFGAGGVSVAIGELAPGLKINLDKVPKKYAGLDGTEIAISESQERMAVVIDPKDREQ
                     FLKYAAEENLEATEVAVVTEDPRLVLSWRGKEIVNISRAFLDTNGAHQENDVFVEIPK
                     AEDTPLKRSGDIADVKEKWLSTLSDLNACSQKGLVEMFDSSIGAASVLMPYGGKYQLT
                     ETQAMVAKVPVPDGKTNTVTMMSYGFDPYVSSWSPYHGAVYAVTESLSRIVAVGGDYS
                     KVRFTFQEYFRRMTEDPHRWSQPFAALLGAYDAQMGYGLPSIGGKDSMSGTFNHIDVP
                     PTLVSFAVDVAKLKDVVSPELKNTGDKLVWIHLPVDAHLLPEYEGVMETYRKLHEDMQ
                     NGRVKAAYVVDRQGIAAAVSKMAFGNALGVTIEHNVDERDLFTPYIADLICEVPAEKV
                     GELASTYTVIGEVTDKPVLSYKDTEITIREAVSAWNKPLEKVFKTVSGAELPEVDALN
                     VAAADENGIVADSCYQAKSIHVCSHKLAQPTVFIPVFPGTNCEYDSTRAFERAGAKVI
                     TQVFSNLSAEDIRGSVDAFEKAINQAQIIMFPGGFSAGDEPDGSAKFFATAFQNAKIK
                     EAVTKLLNERDGLALGICNGFQALIKLGLVPYGEILGQTEDSPTLTFNTIGRHISKMV
                     YTKVVTNKSPWLAGAELGGVYTNPASHGEGRFVASKEWIDKLMANGQIATRYCDVNGN
                     LTNDEYWNVNGSYCGIEGITSPDGRVFGKMAHAERRDNGVAVNIYGEQDLKIFESGVA
                     YFK"
BASE COUNT         2827 a         3143 c         2815 g         3736 t
ORIGIN      
        1 ccaatctcgg aagaaaagcc aagttcttct gcccgcactg cttcaaggaa ctgttcaaat
       61 gttgtctgaa attctcttac ctgttcgata tttccgttta atagaaaatg gcctctgtgt
      121 ttcatcatat gatgcagagc aagatatatc aggcgaatat caggcgtttc gtcagtttcc
      181 attaaccatt ttctcagatg atagatggtt ggaaactgtt tatgatattc tttgtctgtg
      241 taatcatcat ccgcaaacaa tgcataggga agttccggac actttccctg cctgtcacgc
      301 ttatcctctg gccagtaacg actctctttc attctgcgaa aaaatccatc atctatcttt
      361 tctacttctt ccgcaaaaat tttctgcagc aattctattc tccaattacg acgatccaat
      421 cttcgttttg ctgtccgaaa ccctcttctc tcttctgcag tctgtgcact gtcaaatagc
      481 cgtacacccc aaagtgtttt tccatgtgcg cgcaaaattt cgtacttctc atttgtcaca
      541 gcccatccaa ccgaactgct tcccatatca agtccgagat aatattcccc tttcatctgt
      601 tctttcctcc attttctaca ttttatctaa ttttaccata tattttgatt ttttacatct
      661 tttctttcct attttcaaca attctacttc ctggttcttg ctgttctcac agtactgaat
      721 cataaccatg tttcatttct aattttagac aacaaaaaaa ctgctgtcta tgaagacaac
      781 agttttcgtt ccacacactg tggaccatct aacgatggcg ttttcgataa atcgttattt
      841 gaacttatga tgtaaaattt agtatggtgc taaactatct acactatact tctatttttt
      901 cctttcgtca atctttttta gaaagttttt atgataatta ttccattttt ttctcttgcg
      961 tccttcttgt ttttatggta aaatatatct tgttctgatt acgaacactg ccgctgtggc
     1021 ggaattggta gacgcacttg actcaaaatc aagcggtgaa taaccgtgcc ggttcgagtc
     1081 cggccagcgg catagctaaa aaagatctgc aaaaacgaac gttgatgcag atctttttta
     1141 ttgtcttccc ttcaaaaaaa cagcttttga aagaaagaaa acccctttcg aagaaccggc
     1201 catctgccgc atggttcttt ttgaaagggg ttttactgtt gttatttctt atttatttcc
     1261 aaacatcttc tcatactccg gtgttccatc cagtgtagcg aagtaatcct tcggggtttc
     1321 tgcgcggcgg atgacttcaa agctgccgtc ctcttttaac agaacctctg cagattttaa
     1381 ttttccgttg tagttgtatc ccatggaaaa tccatgtgcg ccggtgtcgt ggatgaagag
     1441 gaggtctccg acttccactt ccggaagcat gcggtcgatc gcgaatttat cgttgttttc
     1501 gcacagggaa cctgttacgt catatttgtg gtcgcacggc tgatcttctt ttcccatcac
     1561 cgtgatgtga tggtaagcgc cgtacatagc cggtctcatc aggttgaccg cgcaggcatc
     1621 acagccgatg tactctttgt agatgtgttt ctgatggatg gcgcgggtaa cgagtccgcc
     1681 gtacggtccc atcatgaaac ggcccatctc cgtgtaaatt gcaacatcgc ccatgccggc
     1741 cggaacgagg acttcatcgt acactttatg tacaccctcg ccaatcacgc ggatgtcatt
     1801 gccttcctga tccggtttgt acggaattcc gacgccgccg gaaagattga tgaatttaat
     1861 cttcacgccc gtctctttct gcagctttac agccagctca aacagtgttt ttgccagaac
     1921 tgggtagtaa tcattggtta cggtattact tgcaaggaag gaatggatgc cgaactcttc
     1981 tgcccctttt gccttcagaa tgcggaatgc ctcaaaaatc tgctcggttg tcataccgta
     2041 tttggcatct cccggattat ccatgatgtc ggtgctgatt ttaaacagtc cgcccgggtt
     2101 ataacggcag cagattttct tcggaatgta gcctaatgtt ttctcaagaa aatcaatatg
     2161 tgtaatatca tcaaggttaa tgatcgcacc aagctctgca gccagttgat actctgcagc
     2221 cggtgttacg ttggatgaaa acatgatttt atctttctca aatcccatcg catcggaaag
     2281 catcagctcc gtgtaagaag agcagtcgca tccgcagtca tattcaccca gcattttgat
     2341 cagtgtcggg tttgggttcg ctttcacagc aaaatactca cgaaatcccg gattccagga
     2401 aaaagcttcc tttaccgctt tcgcattctc acgaattcct ttttcatcgt aaatgtgaaa
     2461 cggagttggt actgctgctg cgatttcttc cagtttttct tttgttacaa aaggcttctt
     2521 cgtcattctc ttttgtctcc ttcttctgct tattttgcct gaataatgtg aagcatgttt
     2581 gtcatatatc cttcacccgt cacctcgtta gtagcagggt ctccggccgt aaaaataacc
     2641 atatcgccac gttctacaag ctcctcacgg cgcaccatat acatagcatg ggaaatgatg
     2701 ttctccgtag aatcttcctc gtatccggtc accggacgaa cgccccagta gagctgcatt
     2761 tttcttcttg cccattcata tggggtaacg ccgtaaatcg gtacgctcgg acgaaggttg
     2821 gagatcagtc ttgcggtctg accggacatc gtcggggtca caatgcaggc tgctttgatg
     2881 tgttctacca tagaaacggc tgcacttccg actgctgcgg aaacattcag ctttccttcc
     2941 agcgcttttc catcgcggta atcttcgaat ttcagatagc gctctgtctg ctcggcaatc
     3001 tctcccatca tggtaactgc ctcgatcgga tattttccgg ctgctgtttc gccggagagc
     3061 atgatcgcat ccgttccatc ataaattgca ttcgcaacat cggctacctc tgcacgggtc
     3121 ggacgcgggt tccggatcat ggaatccagc atctgggttg ctgtgataac cggtttgtat
     3181 ttatgattgc atttttcaat gatgattttc tgtacatgcg gtacctggca cgccggaatc
     3241 tctacaccaa ggtctcccct tgcaaccatc acaccatctg ccgcatcgat gatgctgtcg
     3301 atgttctgca caccctccgc attttcgatt ttggcaatga tatcaatatg ctctcctccg
     3361 tttgccacaa ggaactcacg gatttcgtta attgcctctg cattccggat aaaggaagct
     3421 gcaacaaaat caatatcctg ttcaatacca aaaagaagat ctcctttgtc tttctccgtg
     3481 aggttcggaa gattgatcga aacgtttggt acattcacgc cctttttttc tccgagctcg
     3541 ccgccgttta ctacggtaca gacaatttcc ggtgcattta ctttttctac gcgcaggccg
     3601 atcagaccat catcgatcag tattgtgtct ccctctttga tatcttccgt aaggccggtg
     3661 tagctgacag aacagtgatt ttcatctccg gtctcctgaa cggttgtcag ggtaaaagtc
     3721 tgtccggtta ccagatttac tttttttcca tcttttaaaa cgccggtacg aatttccggt
     3781 cctttggtgt ccagaaggat tgccacagga gttttcagct cttttcgaag ctctttcagc
     3841 atatccatac gcattttctg ctcatcgtgg tctccatgag aaaaattaaa acgagcaaca
     3901 tccattccgg tttcaatcag ttttttcatc aggtcacggg agttggtatt tggtcccatg
     3961 gtgcagacaa ttttcgttct tctcatactt tctctccttc tttattttac atgttcatgc
     4021 gtaaacaggt gatcctggca gtattcatag tttccgttgc atcgggaaca gaaacggaac
     4081 tccagattcg gatcatcttt ttcggtccgg ccacagatcg cacatttgtg ttttgtgatc
     4141 ccgttcggat tgcggtattc ggcttcgcct cttgaaacgg cctttttaaa ttccttccgg
     4201 cgtctgttct gctggaaaga gccgcgtttt ccttttcttg tcatcaggaa gaacacaagg
     4261 acgtttgcaa gggaacaaag aataacaaca catcctgccc agtttccgct catgatgctg
     4321 gtaatcatgc tgtaggcgag aagcgctacg tcaaggtatg ccagccattt gattttgatc
     4381 ggaataatga aatacagcag tacctgcata tccgggaagc tgatcgcaaa gccaaggaaa
     4441 atcgagaggc tgatgtagta agtgctgaat gccgttccca tcagcgacgg atatccgttg
     4501 actgcatata agattccata cagcaggaag gaaccgataa tggtcatcag aatgccggaa
     4561 aagatgtaaa cattatactt gaaatctccc caggctctct caaggcttgt tccgattgag
     4621 taatagaaaa acagcatgat gatcgtaaag atatcaaggc tgctcggcgg aatcagaatc
     4681 cagctcacca gacgccagat ctggccatgc agaatatagt atggttccag tgtcagatac
     4741 tgtctcataa tgggggccgc aagcgcaatg atatagcccg caatgtacgt tacaataatg
     4801 tacatcgtca gatggcttaa cgcgtatctc ccatattttc gctccatttt gtctaaaaaa
     4861 ttcatgcaca ctcttccttt ttgtttgttt tatttcaccg gctttcactg cccgaaaata
     4921 cattctcatt atacgtgctg ccttttcgtt tgtcaaacgg tataacggtg aatttcgtct
     4981 ctgaaaaaga gctttacgaa tgttttactt tccggtataa tttattagaa atcttttcca
     5041 gttattttat aaatatacga aaaaattcag gaggacttta tatatggcag gatcatcatt
     5101 tggtaccatc ttcagactga ccacctgggg agagtctcat ggaaaaggcg tgggcgttgt
     5161 tgtggacgga gtgccggccg gcctttcgct ttccgaggag gatattcagg tttacctcga
     5221 ccgcaggaag ccgggacaga accgctttac cacaaaacgg aaagaatccg ataccgttca
     5281 gattttatcc ggcactttcg aaggaaaaac gaccggtacc ccgatttcca tggtggtatg
     5341 gaatgaggat cagcattcca aagattacag cgacattgct tcctattaca gaccgggcca
     5401 cgccgactat acgtttgatg caaaatacgg tttccgcgac taccgcggcg gcggacgctc
     5461 ctcaggacgc gagacgatcg cacgcgtggc tgcaggtgcc attgccttaa aaatgctctc
     5521 tgagcttggc atcaccgttt ccgcctacac ccgctccatc ggtgacgtgg aaatccagtc
     5581 ttttgatgcc gctgaaatcg cgaataacgc cctgaatatg ccggatgcag aggctgccga
     5641 aaaggcttcc gagcttttaa caaaagccat ggcggaaaaa aattccgtcg gcggtgtggt
     5701 cgaatgtgtc gttcaccata tgccggccgg tgtcggcgat ccggtgtttg aaaagctcga
     5761 tgccaacctt gcaaaagcac tcgtctccat cggcgccgta aaaggcgtgg aaatcggaga
     5821 cggtttctcg gtctgcacag ctaccgggct taccaataac gatgccttcc atgtaaatgc
     5881 ggacggctcc attgtcaagc tgaccaacca cgccggcggt attttaggcg gtatcagcga
     5941 cggaagcgat atcgttgtcc gggctggttt caaaccgacc ccgtccgttg ctgcttccca
     6001 gcagaccatc aacaaagacg gcgaaaatat tgccatcgaa atcaaaggcc gccacgaccc
     6061 gatcatcgtt gcacgcgccg ttgttgtcgt ggaatgcatg accgctcttg tcctggcgga
     6121 cgcactgctc gtcggcatga gcgccaaact ggacaatgta aagaaagtct gggctctgga
     6181 cgagaaataa aggccggact gcttctttct ggcttcaaca caaaaaatgc ctgcgggata
     6241 cagttcttcc tgcaggcatt tttgcgttat ttttttagtg aacgcctttt ttcgtaatca
     6301 gaatacagtt cggcgtctcc actttcatcg ggcggccttt ctgaaccagc acgtttttct
     6361 cataatcaac ggtaaatgtt gtgatcgtgt tggactcatg gtttgccact gcgatatgtt
     6421 ttccgtccgg gaacattgca atatcctttg gatattcgcc gctgatcgga agtgcaaact
     6481 tcttctccat gcagccggtc tccgggtcga tatggaacat tgctacggta ttgtctccgg
     6541 ctgtagaagt aaacaggtag tggtgatccg gggaaaggca catgccggat gcagcgtcgt
     6601 gcgcctggtc cacgttcgcg gatgtggtgc tgatcgactg cagtttttca aattccggtc
     6661 cgtttcccgt atcacggtag ctgaatacat cgactttgtt catcagctcg taaatgacat
     6721 acgcgtattt tccggattca ctgaaaacga tctctctcgg accggattca cgctcacagc
     6781 gcaggatatc caccagtttc aggcggtcac gctgtgtgtt cagcttgtac attttgatct
     6841 ggtcaatgcc gttgtcgact gcgcagacat atttgttgtc cggtgtagga acgacacagc
     6901 tgacatgcgg gcggaagtta cgctctgcca cactgccaag gcccttgtgg aacacgccgt
     6961 ccatcagact tccgaggcgg ccgtccttat gggtatgtac aacggttact tttccgtcat
     7021 gatagcctgc cacgaacagg tatcttccgg ttttgtccac cgacagatag cagccgcgca
     7081 tgccgtcgat gtctactttg ttgatcgggg tcaggtctcc gtcatcctcc actgcaaaca
     7141 ctgcaacgcc ttcatctgca atcgaataca ggtatttgcc gtttaaggaa cgggcaatgt
     7201 gggaagagtt gttgaccggt atgacttttc gctctgtcag agtgccttct tctacattta
     7261 catcatataa atgaatacca atgctgcttc catgggtata agtgcctaca taggctacgt
     7321 actcatcctt catgttgttt tcatcctttc tgccttctcg cacatcgtgt gaattttcta
     7381 aaaatatttt aacacaattt ccgcgccacg acaacgagga aaacggattt ttgatatgta
     7441 aacggtaaca ctttattttt agacacagga tattctcctg cagagaattt tttagatatt
     7501 tctattgttt ttagattgta agagttgagg tttccgctgc ataccatttc cattttctac
     7561 gctctcgtca gactgatttc ctgactcatc attccctgca gtttcttgat tgcgcggctg
     7621 tactggcact gcagcgcctg ctcgcttttc ttgcagaatc cgctgagctc tttgtagctg
     7681 aatccatatg ctctccccca gaaaatgcgc tgatccatcg gattcaggtt ctggatgcag
     7741 ttctgcatca ccattcgttc ctcaatctcg gctgtcgcgg ccgcctcccc ttcatttctg
     7801 ttctctttct gatactgtgt ataggaaact tcattctgat aataatgttt tctgcgctga
     7861 ttcttgatct tgttttttgc cacacagaac agccatttcg tcatctcttc tccttcgaga
     7921 tcggcaacgg tttcaaattt taccaccaat acctcccata catcgttcag cacatccttc
     7981 gccgtctccc ggtcattgtt caactgaccg cagatataac ggaacagcac atgactgttt
     8041 tcctcgaata tgatctgaaa aaatcgttcc tcctcttgtc tcatacactt ctttctccct
     8101 cctgttattt cgctgtcctt ctcttccggg cccgtcatat atgtaatgtg ttgagactat
     8161 cgaaatcttt cgttttttca gaatatttct actatttgac cttattactt ttgttaataa
     8221 agcaaaatat tgcgttataa ctgcaccgcg gcgcataatc aaattttttc tttcgaaatc
     8281 agaaattact gtttctgaca aaaaattccc gcagaaagga gctttcttcc tctctgcggg
     8341 aatcttctat tttggtttta ttttatttaa agtaagcaac accggactca aaaatcttca
     8401 gatcctgctc tccgtagatg ttgactgcta cgccgttgtc gcggcgctct gcgtgtgcca
     8461 tcttaccaaa gacacggccg tccgggctgg tgataccctc gattccgcag taggaaccgt
     8521 tgacattcca gtactcgtcg ttggtcaggt ttccgttgac atcgcagtag cgggttgcaa
     8581 tctggccgtt tgccatcagc ttgtcgatcc actctttcga tgctacgaaa cgtccctctc
     8641 cgtgggatgc cgggttggtg taaacaccgc caagctcggc gcctgccagc catggggatt
     8701 tgttggtcac aaccttcgtg taaaccatct tggaaatatg gcgtccgatg gtgttgaagg
     8761 tcagagtcgg ggaatcctcc gtctgtccga gaatctcacc gtacggaacg agtcccagtt
     8821 tgatcagtgc ctggaatccg ttgcagatac caagggcaag tccgtctctc tcattcagca
     8881 gtttggtaac agcttctttg atcttcgcat tctggaatgc ggttgcaaag aattttgctg
     8941 atccatccgg ctcatcacct gcggagaatc cgccagggaa catgatgatc tgcgcctggt
     9001 tgatggcttt ttcaaaggca tcgacagaac cgcggatgtc ttctgcggaa aggttgctga
     9061 atacctgagt gataactttt gcaccggcac gctcgaaagc tctggtgctg tcatactcac
     9121 agttcgttcc cgggaagacc ggaatgaata cggtcggctg tgccagcttg tgggagcata
     9181 cgtggatgct ctttgcctga tagcagctgt ctgcaacaat tccgttttcg tctgctgctg
     9241 ccacattcag ggcatctacc tccggaagct ctgcaccgga tacggtcttg aatacttttt
     9301 ccagcggttt gttccatgcg gaaacggcct cgcggatggt gatttcggtg tctttatagg
     9361 aaagaaccgg tttgtctgtc acttcaccga tgacggtgta ggtgcttgcc agctctccga
     9421 ctttttcagc cggaacttcg cagatcagat ctgcaatata tggagtaaag agatctctct
     9481 cgtctacatt gtgctcgatg gttacgccga gagcattacc gaatgccatc ttggatacgg
     9541 ctgctgcgat accctgacgg tctacgacat aagcggcttt cacacggccg ttctgcatat
     9601 cctcatgcag ttttctgtag gtttccatga cgccttcgta ctctggaagc agatgtgcgt
     9661 cgactggaag gtggatccac acgagtttgt ctcctgtgtt tttcagctcc ggagatacta
     9721 catcttttaa ttttgcaacg tcaaccgcga aggatacgag tgtcggcggt acatcgatat
     9781 ggttgaaggt accggacata ctgtcttttc ctccgataga cggcaggccg tagcccatct
     9841 gtgcgtcgta tgcaccaaga agtgctgcga acggctggct ccagcggtgc ggatcctcag
     9901 tcattctgcg gaaatattcc tggaaggtga agcggacttt gctgtaatcg ccgccgactg
     9961 ccacgatgcg ggacagggac tcggttacgg catagactgc tccgtgatac gggctccagc
    10021 tcgatacgta tggatcaaag ccgtagctca tcatggtaac ggtgttggtt tttccatccg
    10081 gaaccggcac ttttgcaacc atggcctgag tttcagtcag ctgatatttt ccaccgtacg
    10141 gcatcagcac gctggctgct ccgatggagg agtcaaacat ctcgacaaga cctttctggg
    10201 agcatgcgtt gaggtctgac agggtggaca gccatttttc tttcacgtcg gcaatgtcac
    10261 cgcttctttt taacggggtg tcctctgctt ttggaatctc tacgaagaca tcattctcct
    10321 gatgtgctcc gttggtatcc aggaatgcac gggaaatatt gacgatctct tttcctctcc
    10381 aggagagtac cagacgcggg tcctcggtta cgactgcaac ttcggttgcc tccaggttct
    10441 cttctgcggc atatttcagg aactgctcac ggtctttcgg gtcgatgaca acggccatac
    10501 gctcctggga ctcggagatg gcgatctcgg ttccgtcgag gccggcatat tttttcggta
    10561 ctttgtccag attgatctta aggcccggtg ccagctcgcc gatggcaacg gaaactccgc
    10621 ctgcaccgaa gtcgttgcat tttttaatca gaaggctgac ttcctcgcgg cggaacagtc
    10681 tctgcagttt acgctcagtc ggtgcgtttc ctttctgtac ctctgcaccg caggtctcga
    10741 tggattctgt cgtatgtact ttggaggaac cggttgcacc gccgcagccg tctcttccag
    10801 tacggccgcc gagcaggatg atgatatcgc ccggatcgga attttcacgg acaacggcac
    10861 ggcgcggtgc tgctccaagg acagctccga tctccatacg ttttgccacg tagtccggat
    10921 ggtaaacttc tttgacatag ccggttgcaa gaccgatctg gtttccatag gagctgtagc
    10981 catgtgctgc ttctcttaca agttttttct gcggaagttt tccttcgagt gtctcgctct
    11041 gcggacgggt cggatctgcg gctccggtca cacgcatcgc ctgatataca taggttctgc
    11101 cggaaagcgg gtcacggatt gcaccgccaa ggcaggttgc tgcaccaccg aacggctcga
    11161 tctcggtcgg gtggttgtgg gtctcgtttt tgaagttgat cagccactct tcgtcttttc
    11221 cgtctacatc taccggtacg acgatggagc atgcgttgat ttcatcagac tcttcctgat
    11281 ctgcgagttt tccttcggat ttcagttttt tcattgccag aagggcaagg tccataaggc
    11341 agatgaattt gtcgtcacgg cctttgtaga gtacctcacg gtcgcttaag tagcggtcgt
    11401 agcttttttt catcggctcg tagtagtcgc cttcgccgaa ggtcacgttg gtaagctcgg
    11461 tggagaaggt ggtatggcgg cagtgatcgg accagtaggt atcgagtacg cggatctcgg
    11521 tcatggatgg atttcgtttt tcttcgccgc ggaagtagtt ctggatatgc aggaagtctt
    11581 tgaaggtcat ggcaaggccg agggaatcat acagggcttt taattctgcc tccggcatgt
    11641 cgatgaatcc gtcgaagatt ttgacgtctg ccggctcttc aaattttgtc acgagggttt
    11701 ccggtttttc aagtcctgtc tctctggagt ctaccggatt gatgcagtgt gctttgactg
    11761 cgtcaaactg ctcatcagtc atctctcctt cgataacata cgtaacggcg gagcggatga
    11821 tcggctgctc gtcttctttt aagaactgca cgcactgtac ggcagaatcg gctctctgat
    11881 caaactgtcc cggaaggaat tctacggaaa atactctcgc accttctgca cgcgggaaat
    11941 cttcctgata gcagatatca accggcggct ctgcaaagac tactttacat gctttctcat
    12001 atgtctcatc actgatattt tctacatcgt accggatcag ctcccgtacg ttggttacgg
    12061 tctggatgtt cagatagtgc ttgatttcat gcttcagatc tttcgcctta atcgcaaatt
    12121 ccggcttttt ctccacataa acgcgtctga ctgtactcat ttttatcctc catttgtctg
    12181 aatcatagtc ccccaaaaac gggacaatct ttcgttactt acagaatagc atatttatct
    12241 ttataaatga aattaatata tcttatatgt tttataagtt ttgtttctta atagacggac
    12301 gcatttcatt tttcctgcac taccatccca ctcatcaccc attctctccc gtcctaaacc
    12361 gtcaggcccg cgcactttct ggtgctgggg gatgcatttg cagggggatt tagaaatact
    12421 tagagccttg aaatttgcgt tgttataaaa aagtcaactg tctgagcgca gcgagttttg
    12481 acttttttat aacattttta gcaaattcca aggctcttaa t
//
LOCUS       DAOMUF010000046        12424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1161_length_12424_cov_3.115692, whole genome shotgun sequence.
ACCESSION   DAOMUF010000046 DAOMUF010000000
VERSION     DAOMUF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1161_length_12424_cov_3.115692"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLN77_04210"
     CDS             <1..563
                     /locus_tag="PLN77_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRM43518.1"
                     /translation="ARCGNPAAMEKRGIWTLQVYRAFPSPPDFHIWLPITGFSTSDVT
                     FLEPDPYTTLTTPSATVPVLSPSTYQAANNSFSPESGRGFTRLGEIKPDFAAPGVSVT
                     GPGPAGSYVSFSGSSASAAITSGAAALFLQWGIQRTPARYFTAQEVKNYLIRGADRTD
                     TITYPSREWGYGRLQLYQSFTSLMTN"
     gene            710..952
                     /locus_tag="PLN77_04215"
     CDS             710..952
                     /locus_tag="PLN77_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM43519.1"
                     /translation="MNYERLNDLRLLGLTIAYYRRAKGMTQAELAEAVHISRTHMSNI
                     EAPNTKTSISLNLLFDIAEALDIPVKELFDFQGRSL"
     gene            1045..1329
                     /locus_tag="PLN77_04220"
     CDS             1045..1329
                     /locus_tag="PLN77_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorion class high-cysteine HCB protein 13"
                     /protein_id="HRM43520.1"
                     /translation="MSDLAATNCGCGCDNGCGCDNGCGVSNNNLFGNNGCSIIWILLL
                     LSCCGGCGCGNNNNGCNSDCWLVLILLFCCGGWNNNGCGCGNSNNCGCGC"
     gene            1706..2068
                     /locus_tag="PLN77_04225"
     CDS             1706..2068
                     /locus_tag="PLN77_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43521.1"
                     /translation="MNQEQEQNVEQMTPLDQMIAQDQLQMLKAAIPYASFGLQPSLAI
                     LSKWLELQRTIQLFSRPADLSMMSVDAPHVSPLEMLQDISRFAAGPQRDLLSQMFQTM
                     QMVQMLQNINPGNEEDLL"
     gene            2065..2364
                     /locus_tag="PLN77_04230"
     CDS             2065..2364
                     /locus_tag="PLN77_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43522.1"
                     /translation="MSSDDKQNDWSQDPSLSGIDFARLQALQQMASQSRGKSQAEMMP
                     FLLSLMQGNQGGAQFSASEADLIISAIKAGKSKQEAAKIDQMVRLFRSAASGKKR"
     gene            complement(2441..2977)
                     /gene="pgsA"
                     /locus_tag="PLN77_04235"
     CDS             complement(2441..2977)
                     /gene="pgsA"
                     /locus_tag="PLN77_04235"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRM43523.1"
                     /translation="MNLPNKLTILRVIMIPFFVFFLLSGVGGSASKWIALVIFAAASI
                     TDTLDGYIARRDNLITDFGKFMDPLADKLLVCSALICFVELDKLPAWMVIIIIAREFI
                     ISGFRLVASDNGIVIAASWWGKSKTISQMIMIILLIADIPALSVLNTVLIWVALILTV
                     VSLIDYLVKNKNVLSMQN"
     gene            complement(2974..4299)
                     /gene="rimO"
                     /locus_tag="PLN77_04240"
     CDS             complement(2974..4299)
                     /gene="rimO"
                     /locus_tag="PLN77_04240"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRM43524.1"
                     /translation="MKILFISLGCDKNLVDSEHMLGLLVEHGFEITDSEEDAEVIVIN
                     TCCFIHDAKEESIQNILEMAKYRTAGSCKVLLVTGCMAERYRDEIIEEIPEVDAVLGT
                     NSYDKILDAIQDVLGGDRFQEYADLKGLPKIDAQRVVTTGGHYEYLKIAEGCDKHCTY
                     CIIPKLRGPYRSVPMEELIAEAKTMAEQGVKELVLVAQETTIYGIDLYGKPSLHLLLK
                     ELCKIQELYWIRILYCYPEDIYPELVQTMKEEPKVCHYLDLPIQHANDEILRRMGRRT
                     SKQELIDKIDFLRSEMPDITLRTTLITGFPGETKEQHKELLEFINDMEFDRLGVFTYS
                     PEEGTPAATMEHQIDEEVKLDRQAELMELQQDISAELGERRIGQELLVMIEGKVADED
                     AYVGRSQADAPGVDGYVFVNTPETLVSGDFVRVKITGALEYDLIGEISQ"
     gene            complement(4301..4579)
                     /gene="rpoZ"
                     /locus_tag="PLN77_04245"
     CDS             complement(4301..4579)
                     /gene="rpoZ"
                     /locus_tag="PLN77_04245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRM43525.1"
                     /translation="MIHPSYQELIATINEGQDLDEAPVVSSRYSLVLATSKRARQIIG
                     GAEPLVKVKKGMKPLSIAVEEMYKGEVTILPESEVEAEEETKEEPVIE"
     gene            complement(4595..5209)
                     /gene="gmk"
                     /locus_tag="PLN77_04250"
     CDS             complement(4595..5209)
                     /gene="gmk"
                     /locus_tag="PLN77_04250"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRM43526.1"
                     /translation="MSRGILTVVSGFSGAGKGTLMKRLMEEYDNYALSISVTTRDPRP
                     GEEDGVSYFFRTEEEFDRMVEEDAFLEYARYVKHSYGTPKAYVEEQLDAGKDVLLEIE
                     IQGALQVKEKRPDTLLVFITPPTAEELERRLRGRGTETEEVIKGRMERAKEEAQGMDQ
                     YDYVLINDDLETCVKQMHETIQSQHFMSSNQKDFIEQMKKSLEK"
     gene            complement(5202..5480)
                     /locus_tag="PLN77_04255"
     CDS             complement(5202..5480)
                     /locus_tag="PLN77_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HRM43527.1"
                     /translation="MAKLINIGFGNVVNSDKIIAVVSPEAAPVKRMVQRAKESNRIID
                     ATQGRRTKAVLITGEEFIILSALQPETISRRFNGGWETEKERGESENE"
     gene            5632..7374
                     /locus_tag="PLN77_04260"
     CDS             5632..7374
                     /locus_tag="PLN77_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HRM43528.1"
                     /translation="MAFDGITIANIAQELNQTIVGGKINKIAQPENDELMITIKNNRT
                     VYRLLLSASASLPLIYLTDNNKPGPMTAPNFCMLLRKHIGSARILSVTQPGLERILIF
                     ELEHLNELGDICRKKLIVEIMGKHSNLIFCQEDNTILDSIKHISANMSSVREVLPGRT
                     WFIPHTQDKKDPLTITEEEFFDFVCTKPMAAAKAIYTSLTGFSPLMAEEVCCRAGIDG
                     GFAVNALEENAKLHLFRTFSYLIEDIKNGAFTPNIVYQGDEPKEFSALKLTQYQDLRE
                     ETYDSISGVLETYYAMKNRITRIRQKSSDLRRVVNTALERNVKKYDLQQKQLKDTDKR
                     EKYRIYGELLNTYGYSCQPGDTSLKAVNYYNGEEITIPLDGTLSAGENAKKYFDRYGK
                     LKRTREALEELLVETKEEIDHLESISTALDIALDEADLVQLKEELIEYGYIRRKGPTG
                     KKQKITSHPFHYRSSEGFDIYVGKNNFQNEELSFKFASGNDWWFHAKGQPGSHVIVKT
                     NGKELPDSVYEEAAALAAYYSKGRQAPKVEIDYTLKKNLRKPNGSKPGFVVYYTNYSM
                     MAEPNIRNLQEVQD"
     gene            7590..10058
                     /locus_tag="PLN77_04265"
     CDS             7590..10058
                     /locus_tag="PLN77_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRM43529.1"
                     /translation="MNKTIPEIIHRRVPTTRYEADPSEGLSAEQVAEYRENGWTNRAV
                     EPPSKTTAEIVKSNVCTYFNLIFLIIAIFLILAGSFRDLTFLPVIIANTLIGIVQEIK
                     AKKTLEKLSVLNAPKATAIRDGAEITLAAEDLVLDDIVVFTAGKQICADAVVLEGEVQ
                     VNESLLTGESDEITKRPGDELMSGSFIVSGSCKSRLTQVGEDSYISRLTLEAKAMKEG
                     EQSEMILSLNKLVKVVGILIIPIGLLLFGQQFFVSHETFRESITSMVAAVLGMIPEGL
                     YLLASVALAVSVMRLASQKVLVHDMKCIETLARVNVLCVDKTGTITETNMKVHDLILV
                     GSSSVPDTESGENAPAPQKEELSLAVGDFASAMSNDNITMAALKEYFKDNNGKTAVSM
                     TSFSSEFKYSSVTFSDVSYVLGAPEFVLRDDFARYQSEIEAYTSKGFRLLVFGTSETV
                     PDGKALSGRVTPLGYVLLSNPIRKEAPETFRYFEDQGVHVKVISGDTPVTVSEVARQA
                     GIAHAEDYIDASTLKTPEELEEAILKYTVFGRVTPDQKRQFVQALKKNGKTVAMTGDG
                     VNDVLALKDADCSVAMASGSDAAAQAAQLVLLDNDFSKMPSVVLEGRRVVNNLQRSGT
                     LFLVKNIFSFLLSIFSLISMVTYPLEPSQISLISMFTIGIPGFLLSLMPNKNRIEGHF
                     ITNILTRALPAALTDFLMVATLVVFGQEFAVGSEDISTAATVLLAIVGFMILYRISKP
                     LNWMRWTILIGSIIAFIFCSTYLNQLFAISDMSRKCIMLLVVFSVATEPVLRYLSILV
                     DSISSFYRKQKIFFLRKREARKAK"
     gene            complement(10148..10711)
                     /locus_tag="PLN77_04270"
     CDS             complement(10148..10711)
                     /locus_tag="PLN77_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HRM43530.1"
                     /translation="MDLETIRQEARAVAEELITTAQMKPGQILVVGCSSSEIHNSKLG
                     TDSSQEIGQAVFEALYACTKEHGLYLAAQCCEHLNRAVVLERAAAEKYGYEPVCAVPW
                     LHGGGSFAMAAYYGLEDAVTVERVQAHAGIDIGDVLIGMQLKAVAVPVRVSQKTIGDA
                     HVVCARTRPKYIGGERTHYAEDPEMRK"
     gene            complement(10806..>12424)
                     /gene="leuS"
                     /locus_tag="PLN77_04275"
     CDS             complement(10806..>12424)
                     /gene="leuS"
                     /locus_tag="PLN77_04275"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRM43531.1"
                     /translation="EHPYIDQYKDEIKNWDEVCAYREMAARKSDFERSELAKDKTGVM
                     IDGLSAVNPVNGKEIPIWISDYVLMSYGTGAIMAVPAHDERDWEFAKKFNMPIIEVVA
                     GGDVQNEVYTDVAEGTLVNSGFLNGLSVAEAKKKILEWLEENHVGKAKTNYKLRDWVF
                     SRQRYWGEPIPIVHCDKCGYVPVPEESLPLELPDVESYMPTDNGESPLAAMTDWVNTT
                     CPCCGGPAKRETDTMPQWAGSSWYYIRYTDPKNKEALASKEAMKYWLPVDWYNGGMEH
                     TTLHLLYSRFWHKFLYDQGVVTCPEPYQKRTSHGMILGENGEKMSKSRGNVVNPDDIV
                     AEFGADTLRTYEMFIGAFDLSASWSQEGVKGCRRFLERVWKLQDSLVDGDSYSKELES
                     KMHQTIKKVSSDYESLKYNTAIAAMMTLVNEFYKAGKVTRKEFETLLILLNPVAPHMT
                     EELWADLGYEGRLYQTAWPEFDEEKTVEAVAEIAVQINGKMRGTIKIAKDADKETAIA
                     AAKEAVADKLTGNIVKEIYVPGRIVNIVQK"
BASE COUNT         2874 a         3374 c         2875 g         3301 t
ORIGIN      
        1 gagccagatg tggaaatccg gcggcgatgg aaaagcgcgg tatctggacg cttcaggttt
       61 accgcgcttt tccatcgccg ccggatttcc acatctggct cccgatcacc ggattttcca
      121 cctccgatgt gacatttctg gaaccggatc cgtacacgac actgacgaca ccttctgcca
      181 cggttcctgt tctttctccc tccacgtatc aggcagcaaa caacagtttt tccccggaat
      241 ccggccgcgg ctttacccgg cttggtgaaa tcaagcccga ctttgctgct cccggcgtct
      301 ccgttaccgg accgggaccg gccggctcct atgtaagctt cagcggttcg agtgcgtccg
      361 ccgccattac atccggcgcc gctgctcttt ttctgcaatg gggcatccag cgcactcccg
      421 cccgctattt cacggcacag gaggtaaaaa actacctgat ccgcggtgca gaccgaactg
      481 atactattac gtatccgagc agagaatggg gatatggccg attacagtta tatcaaagtt
      541 tcacaagcct tatgacaaac tgattttccc gctcacaggt ttgcttctat ggtatatccg
      601 tttgttgatg caaactttca atgtgcaact gccttgtttt tgattgtttt ccgttgtatc
      661 attacgtatt atcattatat ttataaaact aaaaggggga ttcctgacta tgaattacga
      721 acgcttgaac gatcttcgcc ttcttggact tacgattgcc tactaccgaa gagcaaaagg
      781 aatgacgcag gccgaacttg ccgaggcggt acatatcagt cgtacccata tgagcaacat
      841 cgaagcgcca aatacaaaaa cctctatttc gttgaatctt ctgttcgata tcgctgaggc
      901 acttgacatt ccggtgaaag agctttttga ttttcaggga cgcagtctgt agggtctttg
      961 aatttttctc tttaagagta accccgcccg gcagtttttc atatcatgaa ttgtaagcaa
     1021 aatgattcct ttggaggaac aaacatgagt gatttagctg ctacaaactg tggatgcgga
     1081 tgtgacaacg gctgtggctg cgataatgga tgcggtgttt ccaacaacaa cctgttcgga
     1141 aacaatggct gcagtattat ctggattctg cttctcctct cctgctgcgg cggatgtgga
     1201 tgcggaaaca acaacaatgg ctgcaattcc gactgctggc tcgttctgat tcttctgttc
     1261 tgctgcggcg gatggaacaa caacggctgt ggatgcggca acagcaataa ctgcggatgc
     1321 ggctgctgac cttttttcag tcctcccaga tgttcgggca gaaaaagccc cgggtgcgga
     1381 tcaaccgccc cggggctttt tttcaccaga attctctttc agcgaaaccg tttcggtttc
     1441 cgtgcttctc tttcccgctt ttccatctct tctctttttt ctttttttct catacattcc
     1501 agatctattt cataaaaggc attcccttcg agtacctgtt ttctttttgc tgtttctttt
     1561 ccatccattt tccggcggtc tcctttttct ttcttcctct tatcatatgt tttttcccgc
     1621 cttctgtcat tttttccggc ctggaacata agataaagag aaataaaaag acaaatgcag
     1681 gaatgctgcc aggaaggatc ttaatttgaa tcaggaacag gaacaaaatg tggaacagat
     1741 gacaccgctg gaccagatga tcgcacagga tcagcttcag atgctgaagg ctgccattcc
     1801 ctacgcctcc ttcggcctgc agccctccct tgcgattttg agcaaatggc tcgagctcca
     1861 gcggaccatt cagctttttt cccgtccggc cgacctttcc atgatgtctg ttgatgcccc
     1921 gcacgtctct ccgcttgaga tgctgcagga tatttcccgt tttgccgccg gtccgcagcg
     1981 ggatcttctc tcccagatgt ttcagaccat gcagatggtc cagatgcttc agaatataaa
     2041 tcccggaaat gaggaggatc tgctatgagt tcggatgata agcaaaacga ctggtcgcag
     2101 gacccctctc tttccggaat tgactttgcc cggcttcagg cactgcagca gatggcctct
     2161 cagagccgcg gaaaatccca ggcggaaatg atgccgtttc ttctctccct gatgcaggga
     2221 aatcagggcg gcgcacagtt ttccgcctcc gaggcggacc tgatcatctc tgccatcaaa
     2281 gccggaaaat ccaaacagga agcggcaaaa attgaccaga tggtacgcct cttccgctcc
     2341 gctgcttccg ggaaaaaacg ctgaaaaact acaaaccaaa aaacgctgcc ggtctggaat
     2401 cccaaaccgg tcagcgtttt tattctgtgc tttttcctgc ctagttctgc atggaaagca
     2461 cgtttttatt ttttaccagg taatcaatca gagaaacaac ggtcagaatc agtgcaaccc
     2521 agatcagcac ggtattcagc acggaaagtg ccggaatgtc ggcgatcaga agaatgatca
     2581 taatcatctg gctgattgtt ttcgattttc cccaccagct tgctgcgatg acaattccat
     2641 tgtccgaagc aaccaggcgg aaaccgctga tgataaattc tcttgcgatg ataataataa
     2701 ccatccacgc cggaagcttg tccagctcta caaagcagat cagtgcagag cataccagca
     2761 gcttatctgc cagcggatcc ataaactttc cgaaatcggt aatcagattg tcacgtcttg
     2821 caatgtagcc gtccagcgta tccgtgatgc tggcagcggc aaagattacc agtgcaatcc
     2881 atttggacgc gcttccgccc acgccagaca gcaggaagaa cacgaaaaac ggaatcataa
     2941 ttacccgcag aatcgttaat ttgtttggta aattcattgg cttatctctc ctatcagatc
     3001 atattccaga gcgccggtaa ttttcactct tacgaaatcg ccggatacga gtgtttccgg
     3061 tgtattgaca aatacatagc cgtctacacc cggtgcgtcc gcctggcttc tgcccacata
     3121 ggcatcctca tccgccactt tgccctcgat cataacaaga agctcctggc cgatcctgcg
     3181 ctcgccgagt tctgcagaga tatcctgctg cagctccatc agttctgcct gacggtccag
     3241 ttttacctcc tcgtcgatct gatgctccat cgtagcagcc ggtgttccct cctccggaga
     3301 gtaggtgaat acgccgagac ggtcgaactc catgtcgttg ataaactcga ggagttcttt
     3361 gtgctgctct tttgtctctc ccgggaatcc tgtgatcagc gtggtgcgca gtgtgatatc
     3421 cggcatctcg cttctcagga aatcaatttt gtcgatcagc tcctgtttgg atgttctgcg
     3481 acccatgcgg cgcaggatct catcgttggc atgctgaatc ggcagatcca ggtagtgaca
     3541 gactttcggc tcttccttca tcgtctgaac gagttccgga tagatgtcct ccggatagca
     3601 gtacagaatc cggatccagt agagctcctg aattttgcag agctctttta acagaaggtg
     3661 gagagacggc tttccgtaca gatcgatacc ataaatggtt gtctcctgcg caacgagaac
     3721 gagctctttg acaccctgtt ctgccatcgt cttcgcttct gctatcagtt cctccatcgg
     3781 aacggagcgg tacggtccgc gcagcttcgg aatgatgcag taggtacagt gcttgtcaca
     3841 gccctctgca attttcagat attcgtaatg gccgcctgtc gttacaacgc gctgtgcgtc
     3901 aattttcggc agtcctttca gatcggcata ctcctggaag cggtcgccgc cgaggacatc
     3961 ctgaatggca tccaggattt tgtcatagct gttcgttcca agtacggcgt caacctccgg
     4021 aatctcctca atgatttcat cgcggtagcg ctctgccata cagcccgtta ccagaagaac
     4081 cttacagctt cccgctgtac ggtacttcgc catttcaaga atattttgaa tgctttcttc
     4141 tttggcgtcg tggataaagc agcatgtgtt tatcacgata acctctgcat cttcctcgga
     4201 atcggtgatc tcgaagccat gctctaccag aagacccagc atatgctcgg aatctaccag
     4261 gtttttatcg catccaaggg aaataaacag aattttcata ttattcaatt actggctcct
     4321 cttttgtttc ctcttccgct tcaacttcag attccggaag gattgtaacc tctcctttgt
     4381 acatctcctc tacggcgatg gagagcggtt tcatgccttt tttcactttt acaagcggtt
     4441 cagctccgcc aatgatctgt cttgcacgtt tgcttgttgc aagtaccaga gagtaacggc
     4501 tgctgacaac cggtgcctcg tccagatcct ggccctcgtt aatagttgca atcagttcct
     4561 gataagatgg atgaatcata ataaatgctc ctttctattt ttccagactt tttttcatct
     4621 gttcgataaa gtctttctgg ttgctgctca tgaaatgctg gctctgaatg gtctcatgca
     4681 tctgttttac gcaggtttcg aggtcgtcgt tgatcaggac ataatcgtac tggtccatgc
     4741 cctgtgcttc ctcttttgca cgctccattc tgcctttgat aacttcttct gtttccgttc
     4801 cgcgtccccg taggcgtcgt tccagctcct cggcggtcgg cggtgtgatg aacacaagaa
     4861 gggtgtccgg acgtttttcc ttgacctgaa gtgctccctg gatttcaatc tcaagcagaa
     4921 catcttttcc ggcatccagc tgctcctcta cgtatgcctt cggcgttccg taggaatgtt
     4981 ttacgtaacg cgcatactcg aggaaggcat cctcttctac catgcggtca aactcttctt
     5041 ctgtacggaa aaagtacgat acgccgtctt cctcgcccgg acgcgggtct cttgtggtga
     5101 cggagatgga cagcgcatag ttgtcgtact cctccatcag gcgtttcatc agtgttcctt
     5161 ttcctgctcc ggaaaaaccg gatactacag taagtattcc tctactcatt ttcagattcc
     5221 cctcgctctt tttctgtttc ccagccgccg ttgaatctgc ggctgatcgt ttccggctgc
     5281 aaagccgaaa ggatgataaa ttcttctccg gtgatgagca ctgctttcgt gcgtcttccc
     5341 tgcgtcgcat cgatgatgcg gttgctttcc tttgcacgct gcaccatacg cttgacgggc
     5401 gccgcctcgg gactcaccac tgctattatt ttatccgaat ttacgacatt gccgaatccg
     5461 atgttgatca gttttgccac aatccgtcct ttcctgtacc ttctgaagtc tttttcagaa
     5521 aatacatcta cccatacaga tttattataa ttcattcaga aagcctagtc aaatcttttt
     5581 ttctgtatta taatgaaaat actagaattt caaacaaagg agatattttc tatggctttt
     5641 gatggaatta ccattgcaaa tattgcacag gaactgaatc agaccatcgt cggcggaaag
     5701 atcaacaaga tcgcacagcc ggaaaacgac gagctgatga ttacaataaa aaacaaccgt
     5761 accgtttacc gtcttctcct ctcggcaagt gcttcgcttc cgctgatcta cctgacggac
     5821 aacaataagc ccggcccgat gaccgcaccg aatttctgta tgctgctccg caaacacatc
     5881 ggcagcgccc gcattctctc cgttacccag ccgggactcg agcgtattct tatttttgag
     5941 ctcgagcatt tgaacgaact cggcgatatc tgccgcaaga agctgattgt ggagatcatg
     6001 ggaaaacaca gcaacctgat cttctgtcag gaggacaata cgatccttga cagtatcaaa
     6061 catatttccg ccaacatgag ttctgtccgt gaggttcttc cgggccgcac ctggttcatt
     6121 ccgcacacgc aggataagaa agatccgctc acgatcacag aagaagaatt tttcgatttc
     6181 gtctgcacga aaccgatggc tgccgcaaag gcaatctaca cttcccttac cggcttcagc
     6241 ccgctgatgg cggaggaagt ctgctgccgc gccggcatcg acggcggatt tgcggtcaat
     6301 gcactcgaag aaaatgcgaa gcttcatctg ttccgcacct tctcttatct gattgaggat
     6361 atcaaaaacg gcgcatttac gccgaacatc gtataccagg gcgatgagcc gaaagaattt
     6421 tccgcactga aactgacgca gtatcaggat ttgcgggagg aaacatacga ttcgatctcc
     6481 ggtgtactgg aaacatacta tgccatgaaa aaccgcatca cccgcatccg tcagaaatct
     6541 tccgatctgc gccgtgtcgt caataccgca ctcgaacgga atgtcaaaaa atacgacctg
     6601 cagcagaaac agttaaaaga caccgacaag cgggaaaaat accggattta cggtgagctt
     6661 ttaaatacgt acggctacag ctgccagccg ggcgatactt cgctgaaagc cgtcaattac
     6721 tataacggcg aggaaatcac gattccgctc gacggaacgc tctctgccgg cgaaaatgcg
     6781 aaaaaatact tcgaccgata cggaaagctg aagcggaccc gtgaggcact cgaggaactt
     6841 cttgtcgaga cgaaagagga aatcgaccat ctcgaatcca tcagcacggc gcttgatatt
     6901 gcgctcgatg aggcggatct ggtgcagtta aaagaagagc tgatcgagta cggctacatc
     6961 cgccgcaagg gaccgacagg gaaaaagcag aaaatcacct cccatccgtt ccactaccgc
     7021 tcttccgagg gctttgacat ttatgtcggc aagaacaact tccagaacga ggaactttcg
     7081 tttaaattcg cctccggcaa cgactggtgg ttccacgcga aaggccagcc gggcagccat
     7141 gtcatcgtaa agacgaacgg taaagagctg ccggattccg tctacgaaga ggccgctgct
     7201 ctcgccgcct attactcgaa aggcagacag gcaccgaagg tggaaatcga ctacacctta
     7261 aagaaaaacc tgcgcaaacc aaacggaagc aaaccgggct tcgttgttta ttacaccaat
     7321 tactccatga tggcggagcc gaacatccgc aatcttcagg aagtacagga ctaaattcag
     7381 aatacaaaag acttttcgca cattcctatc tgttcctctt tgcgaaaagt ctttttttcg
     7441 taaaatttaa tacttccttc atttcttttt cgatgctcta tggtatactt cttagtatgg
     7501 aaaaagattc cagacgtgat ctttttcaaa caaaccacag tgaggctgtc tcttctttat
     7561 caggacagcc ggagaggaat ataactgtta tgaacaaaac aattcccgaa atcattcacc
     7621 gccgtgttcc aacgacgcgc tatgaggcag acccttccga aggcctttcc gccgagcagg
     7681 ttgccgagta ccgcgagaac ggctggacca accgcgcggt ggaacctccg tccaagacaa
     7741 cggccgagat cgtaaagagc aatgtctgta cgtatttcaa cctgattttt ctgattattg
     7801 ccatctttct gatccttgcc ggttcgttcc gggatcttac gtttctgccg gtcatcatcg
     7861 cgaacacgct gatcggtatt gtacaggaga tcaaggcgaa aaagacgctg gaaaagctgt
     7921 ctgttttaaa cgcgccgaag gctactgcaa tccgtgacgg ggctgagata acactcgccg
     7981 cggaggatct cgtactcgat gatattgttg tatttaccgc aggaaaacag atctgcgctg
     8041 atgccgtcgt gcttgaaggt gaggtacagg tcaacgaatc gctgcttacc ggtgaatccg
     8101 atgagattac caagcgcccg ggcgacgagc tgatgtccgg aagttttatc gtctccggaa
     8161 gctgcaagag ccgtctgacc caggtcggtg aagattccta catttcccgt ctgaccctcg
     8221 aggcaaaggc catgaaagag ggcgaacagt ccgagatgat cctctccctc aacaagctgg
     8281 taaaggttgt cggcattctg atcattccga tcggtcttct gctcttcggc cagcagtttt
     8341 tcgtctccca cgaaacgttc cgtgagagca ttacctcgat ggtggccgcc gtgctcggca
     8401 tgatcccaga aggtctttac ctgcttgcga gcgttgccct tgccgtcagc gttatgcggc
     8461 tggcttcgca gaaggttctc gtgcatgata tgaaatgtat cgagacgctg gctcgcgtca
     8521 acgtgctctg tgtcgataag acgggaacca tcaccgagac aaatatgaaa gtacatgatc
     8581 tgattctggt cggctccagt tcagtccctg atacagaatc cggcgaaaat gctcccgcac
     8641 cgcagaaaga agagctttcc cttgcggtcg gtgatttcgc atctgccatg tcaaacgaca
     8701 acattactat ggcggcactg aaagaatatt ttaaagacaa caacggcaaa actgccgttt
     8761 cgatgacttc gttctcctcg gaattcaagt attccagtgt gacattttcg gatgtctcct
     8821 acgtgctcgg tgccccggag ttcgttctcc gcgatgattt tgcacgctat cagagtgaaa
     8881 ttgaagcgta tacctcaaag ggattccgtc tcctcgtctt cggcacatcc gaaactgtac
     8941 cggatggaaa agcactctcc ggccgtgtca cgccgcttgg ctacgtgctt ctctccaatc
     9001 cgatccgaaa agaagcaccg gaaacgttcc gctacttcga agatcagggt gtccatgtca
     9061 aggtcatctc cggtgatact ccggtaacgg tatctgaggt tgcccgccag gcagggattg
     9121 cgcacgcaga ggattatatt gatgcttcca ctctaaagac accggaagag ctcgaagaag
     9181 caatcctgaa atacaccgtg ttcggccgtg tcacaccaga ccaaaaacgc cagtttgtcc
     9241 aggctttaaa gaaaaacggc aagaccgttg cgatgaccgg cgatggtgtc aacgatgtgc
     9301 tcgcattaaa ggatgccgac tgctccgttg cgatggcatc cggaagtgac gctgccgcac
     9361 aggctgcaca gctcgttctg ctggacaacg acttttcgaa gatgccttct gtcgtactgg
     9421 aaggccgccg cgttgtcaac aacctgcagc gctcgggaac gctgttcctg gtaaagaaca
     9481 ttttctcgtt cctgctgtcc atcttctcac tgatctcgat ggtgacgtat ccgctggagc
     9541 cgtcccagat ctcgctgatc agtatgttta cgatcggtat tccaggcttc ctgctctcgc
     9601 taatgccgaa caaaaaccgg atcgagggcc actttatcac gaacattctg acacgggccc
     9661 tgcctgcggc gctgaccgac tttttgatgg tcgcaacact cgtcgtcttc ggccaggaat
     9721 ttgcggtcgg ctcggaagat atttcgaccg ctgccaccgt actgctcgcc attgtcggtt
     9781 ttatgattct ctaccggatc agcaagccgc tcaactggat gcgctggacg atcctgatcg
     9841 gctcgattat tgccttcatt ttctgcagta cctatctgaa ccagctgttc gccatttctg
     9901 atatgtccag aaaatgtatc atgctgctgg tcgtattctc tgtcgcaaca gagccggttc
     9961 tgcgatattt gagcatcctc gtggattcca tttcctcttt ttaccggaag cagaaaattt
    10021 tcttcctgcg aaaacgggaa gcgagaaaag cgaaataaaa cgtaaaacca aacatcaaaa
    10081 atgccgtaca gcgttggaaa tttttctccg cctgtacggc attttttgat ttaccaatca
    10141 cagtttctta ctttctcatc tccggatctt ccgcgtaatg cgtccgctcg ccgccgatgt
    10201 atttcggtct tgtccgcgcg cagacaacgt gcgcatctcc gatcgttttc tgcgataccc
    10261 ggaccggaac ggctactgcc ttcagctgca tgccgatcag cacatcaccg atatcaatac
    10321 ctgcatgcgc ctgcacacgc tctacggtga cagcgtcctc caatccatag tatgccgcca
    10381 tcgcaaacga tccgccgcca tgcagccacg gtaccgcgca gaccggctcg taaccgtatt
    10441 tttctgccgc cgcacgctcc agaacaaccg cgcggttcag atgctcacag cactgcgccg
    10501 ccagatacag cccgtgctcc ttcgtacacg catacagcgc ctcaaacacc gcctgcccga
    10561 tctcctggct ggaatccgtt ccgagcttac tgttgtgaat ttcactcgaa gaacatccca
    10621 ctaccagaat ctgtcccggt ttcatctgcg ccgtcgtaat cagctcctct gcgacggcac
    10681 gcgcttcctg tctgattgtt tcaagatcca ttttgtatcc ctccttgcta aacagcaata
    10741 tttatgcaaa aggagactga ctctttttcc aagaatcagc ctcctttctt cagcttacat
    10801 aattattatt tctgaacgat attcacaatt cttcccggta cgtaaatttc ttttacgatg
    10861 tttccggtca gtttgtctgc aactgcctct tttgcagcgg cgattgcggt ctctttatct
    10921 gcatctttgg cgattttgat cgttccgcgc attttgccgt tgatctgaac agcgatctcg
    10981 gctactgcct ctacggtctt ctcctcgtca aactccggcc atgcggtctg atacagacgg
    11041 ccttcgtatc caaggtctgc ccacagttcc tcggtcatat gcggtgcaac cggattcagc
    11101 aggatcagaa gggtttcgaa ctcttttctc gttacttttc cggctttgta gaactcgttg
    11161 accagggtca tcatggctgc gatcgctgtg ttgtatttca gggactcata gtcgctggat
    11221 accttcttga tggtctggtg cattttggac tccagctctt tggagtagga atctccgtct
    11281 accaggctgt cctgcagttt ccatacacgc tcgaggaaac gacggcatcc ttttacacct
    11341 tcctgagacc aggatgcact taagtcgaag gcaccgatga acatctcgta cgtacgcagg
    11401 gtatctgcac cgaactcagc tacgatatca tccgggttga caacgtttcc acgggattta
    11461 ctcatcttct cgccgttctc gccgaggatc attccgtggg aagtacgttt ctgatacggt
    11521 tccgggcagg taactacgcc ctgatcgtac aggaatttat gccagaaacg ggaatacaga
    11581 aggtgaaggg tggtgtgctc cattcctccg ttgtaccagt caaccggcag ccagtatttc
    11641 atagcttctt tgcttgccag agcctctttg ttcttcggat cagtatagcg gatgtaatac
    11701 caggaagaac cagcccactg cggcatggta tcggtctcac gttttgcagg tcctccgcag
    11761 cacgggcagg tggtgttgac ccagtctgtc atggcagcca gcggagactc gccgttatcg
    11821 gtcggcatgt agctctctac atccggcagc tccagcggaa gggattcttc cggaaccgga
    11881 acgtaaccgc atttgtcgca gtgtacgatc ggaatcggct caccccagta acgctgacgg
    11941 gagaataccc agtcacgcag cttatagttt gtttttgctt ttccaacgtg gttctcttcg
    12001 agccactcaa gaattttctt ttttgcttcc gcaacggaaa gtccgttcag gaaaccggag
    12061 ttgacaagag ttccctctgc aacgtcggtg taaacttcgt tctgtacgtc gccgcctgca
    12121 acaacctcga tgatcggcat gttgaatttc tttgcgaact cccagtcacg ctcatcgtgt
    12181 gccggtactg ccatgatggc gccggtgccg tagctcatca gtacataatc ggaaatccag
    12241 atcgggatct cttttccatt aaccgggttg actgcggaaa gtccgtcgat catgacaccg
    12301 gtcttgtctt ttgcaagctc ggaacgctcg aaatcggatt ttctggctgc catctcacgg
    12361 tatgcgcaga cctcatccca gttcttgatc tcatctttgt actggtcgat gtacggatgc
    12421 tccg
//
LOCUS       DAOMUF010000047        12320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1174_length_12320_cov_3.469384, whole genome shotgun sequence.
ACCESSION   DAOMUF010000047 DAOMUF010000000
VERSION     DAOMUF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1174_length_12320_cov_3.469384"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLN77_04280"
     CDS             <1..628
                     /locus_tag="PLN77_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRM43532.1"
                     /translation="EGQLGSLKDPDHGIYPMVTSSSTLAGYGAVGAGVPPYEIKKIVT
                     VCKAYSSAVGAGAFVSEIFGDEADELRRRGGDGGEFGATTGRPRRMGWLDCVASRYGC
                     RMQGTTDVAFTVLDVLGYLDEIPVCTAYEVDGKEIHDFPNTSILEKAKPVLKTLPGWK
                     CDIRGIKKYEDLPENCRNYIEFVEKEIGFPITMISNGPGRDDIIYRNK"
     gene            654..1346
                     /locus_tag="PLN77_04285"
     CDS             654..1346
                     /locus_tag="PLN77_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43533.1"
                     /translation="MKKAGQLLAVLTAASVLLSGCEKLKDVTTTSVYVSKNGVVTEAI
                     VEDYSKDDDYTEDELKVFVEDDIKKFTEERGDADSVKLEKCQIKEDKVEIRMEYGDYQ
                     SYADYHGAEFFAGTLDEAEEAGYDFSASFVDSKGNEVSVEDAVKGVKHVRVIVCEEPL
                     EIVTEDPVLCVSGTAVIKGKNTVDTAGEWTTKSTESASASSSEQTKTEETEQEYEQDV
                     LLASPVIVVYGK"
     gene            1374..2771
                     /locus_tag="PLN77_04290"
     CDS             1374..2771
                     /locus_tag="PLN77_04290"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRM43534.1"
                     /translation="MEKTMDKIVAVAKSRGFVYPGSEIYGGLANTWDYGPLGVELKNN
                     VKKAWWQKFVMESPHNVGVDCAILMNPQTWVASGHLGGFSDPLMDCKECHERFRADKL
                     IEDFCEEKGIEIEGGSVDAWSQEEMKNFIEEHQIPCPSCGKHNFTDIRQFNLMFKTFQ
                     GVTEDAKNTVYLRPETAQGIFVNFKNVQRTSRKKIPFGIGQIGKSFRNEITPGNFTFR
                     TREFEQMELEFFCEPGTDLDWFQYWRGFCINWLKSLGMKDEEMRARDHSPEELCFYSK
                     GTTDIEFLFPFGWGELWGIADRTDYDLTQHQNVSGQDMTYFDPEKNEKYIPYVIEPSL
                     GADRVVLAFLCSAYDEEVLDAEKNDVRTVMHFHPALAPVKIGVLPLSKKLNEGAEKVF
                     AELSKKYNCEFDDRGNIGKRYRRQDEIGTPYCITYDFDSETDGAVTVRDRDTMEQERI
                     KIEDLDAYFADKFTF"
     gene            complement(2848..3777)
                     /gene="arcC"
                     /locus_tag="PLN77_04295"
     CDS             complement(2848..3777)
                     /gene="arcC"
                     /locus_tag="PLN77_04295"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRM43535.1"
                     /translation="MKRKKIVVALGHKALGTTLPEQKAAVKRSAKVIADLVAAGADVV
                     ITHSNAPQVGMIHMAMNEFGKMHTDYSAAPMSVCSAMSEGYIGYDLQNAIRAELLRRG
                     IFKTTSTILTQVEVDPYDDAFNNPVKLIGRTLTKEEADAEEEKGNYVTKTEDGYRRIV
                     AAPKPQDIVEIDSIRLLLDAGQVVIAAGGGGIPVLPQNEELKGASAVIEKDLTSGLMA
                     EMLNADMLMILTSVENVSINYGTPDEKPLEHITVADAKKYIAEGQFGENSMLPKMEAA
                     VSFLEKGIGRTAVITSIDSALAGFQGKTGTIIE"
     gene            3975..5450
                     /locus_tag="PLN77_04300"
     CDS             3975..5450
                     /locus_tag="PLN77_04300"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRM43536.1"
                     /translation="MKNMRTLQKRIAYQLQQGSLDAEVTGITCDSRKVQPQNVFVCIR
                     GNRMDGHVYAEEAVERGAAVLVAERELEGIPEKVTVLVVKDSREALAQLSCAWEDDPA
                     QHLVTIGVTGTKGKTTTAYMIYEILRKAGIRAGLIGTIETRIGEKSIPSENTTPEPVV
                     LQKLFSEMMREGCTHVVMEVSSQAEKQHRCDGFTFDYGVFTNIGNDHIGAGEHASFAE
                     YLSCKKKLLRTCRTGIVNRDDKRFPEIIRQSTCRLVTFGCREKADFMAADAALDQAPG
                     VLGVSYRVQGAADFPVRVDLPGLFSVSNSLAAIAVCAQMGIPEKAMQEALSAVRVRGR
                     CEAVPVYPEMTLLIDYAHNAMSLESLLKTLRKYHPKRLICLFGCGGNRARDRRFQMGE
                     VSGRLADLTVITSDNPRDEEPQAILDDIRTGIGKTEGAFVEIADRKEAIRYVIENRQP
                     GDLVVLAGKGHETYQIRKGKRYEMDERVLIREILEESKGNV"
     gene            5447..7675
                     /gene="priA"
                     /locus_tag="PLN77_04305"
     CDS             5447..7675
                     /gene="priA"
                     /locus_tag="PLN77_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRM43537.1"
                     /translation="MSSVYADVIVDITNEKLDRTFQYKVPPELTGEIGPGTAVQIPFG
                     NGNRTITGYVLSVGNEPKYPQDRMKAILAVQKDAATVETKLIALAAWMRHTYGSTMIQ
                     ALKTVLPVKVKARGKETREIALLADPARCEELLENYRKKNYKARIRLLNALLAQPVLP
                     GPVVSKELKITADTLHVMEEQGVLEIRRTSTWRNPVSDAAGRTTAGKPNEQQQAVVDG
                     IRKEWEGQNRPCLIRGVTGSGKTLVYMELMERVLKEGKQVIVLIPEIALTYQNVERFC
                     ARFGTRVTVVNSRMTPSERADQMERARRGEVSIMIGPRSALFAPFPDLGLIVIDEEHE
                     TSYKSEVTPRYHARETAVRRAGLEHAHVVMGSATPSVDASYACETGAYALFRMDARYG
                     NAALPSVWIADMREELQMGNRSILSGKLREEIEKRLEKKEQVVLFLNRRGYAGFVSCR
                     ACGHVMKCPHCDVSLTAHNNGKLVCHYCGYETPQVHACPSCGSPYIGGFRAGTQQIEQ
                     NVKKLFPKARVLRMDGDTTKTKNSYEEILSSFAAGEADILIGTQMIVKGHDFPNVTLV
                     GALAADLSLNAVDYRAGERTFQLLTQAVGRAGRGEKPGMAVIQTYHPEHYSIQTAAEQ
                     DYGTFYEKEMDYRRLMGYPPAAELLAIHGAGEDEAHLTQAMEYIRRYLIRIRGNREVQ
                     IIGPASEAVSKINDLYRMAVYVRAPQEEVLAELREKLERYIEINKGFRTVYLQFDFSR
                     KY"
     gene            7739..8230
                     /gene="def"
                     /locus_tag="PLN77_04310"
     CDS             7739..8230
                     /gene="def"
                     /locus_tag="PLN77_04310"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRM43538.1"
                     /translation="MAIREIRVVGDRVLTKKCRPVEKMTPRISELIDDMFDTMYEANG
                     VGLAAPQVGILKRIVVMDVDGTPHLLINPEILETSGSQTGAEGCLSLPGKSGTVTRPN
                     YVKVRALDRDMQPFELEGTELLARCICHECDHLDGHMYTEKVEGELWDNTYDEYEEYE
                     DEE"
     gene            8258..9229
                     /gene="fmt"
                     /locus_tag="PLN77_04315"
     CDS             8258..9229
                     /gene="fmt"
                     /locus_tag="PLN77_04315"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRM43539.1"
                     /translation="MKVVFMGTPDFSVGTLEALVEAGYEITGVVTQPDKPKGRGKQMM
                     PTPVKEAAEKHGLPVYQPRRVRDAEAIEEIRKMEPDVIVVVAFGQIIPKEILDMPKYG
                     CINVHASLLPAYRGAAPIQWAVMNGDEVSGVTIMKMDEGLDTGDMLTKVEVPLAADET
                     GGSLFDKLAAAGAKLLVETLPKLEKGEVTPEKQPEISTTEYARMIKKEDGKIDWTKSA
                     VEIERQIRAMSPWPSAFTKVNGKNLKIWDAKVIAMFGGDLFSKIPGQNPTKSAGKVWV
                     ADETGLHVKTGDGILVIEELQLEGKKRMKTADFLRGYAIEPGTRLGE"
     gene            9316..10011
                     /locus_tag="PLN77_04320"
     CDS             9316..10011
                     /locus_tag="PLN77_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRM43540.1"
                     /translation="MGLFYDSTYLLVILAALISMAVSARMNTIFSKYQTVRSYSGMTG
                     REAAMRILHQAGIYDVTVRHVSGKLTDHYDPRTKTVNLSDAVYDSTSVAAIGVAAHEC
                     GHAVQHNTDYVPLKVRSAIVPAANLGSQLFWPLFLIGLIFSLPMLVKAGIWLFVLALA
                     FQVVTLPVEFDASRRALKMLSEGGMVTETEHDGVKKVLWAAAMTYVAAVIGSLLQLLR
                     LLILSGAFRRNDD"
     gene            10004..11368
                     /gene="rsmB"
                     /locus_tag="PLN77_04325"
     CDS             10004..11368
                     /gene="rsmB"
                     /locus_tag="PLN77_04325"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRM43541.1"
                     /translation="MTDTINTRELIMNILLEMDREKTPCHLAVRDALDKYQFLPRQDR
                     AFIKRVCEGTVEYGIQNDYIINQYSKIKINKCKPVIRAILRMSVYQIRFLDAVPDSAV
                     VNEAVKLAEKKHFYNLKGFVNGVLRTIVREKDHLPIPKENNTTQYLSVKYSMPEFLVE
                     EFVSQYGKETAETMMADFLKEKQVTIRINRWNNTIEETKKSLESQGAELEKAPYLGEA
                     FYLKNFETIASLNAFQEGRFQIQDVSSMMAAHLADPKPGDQVLDLCAAPGGKSLHAAD
                     KMRNQGCVEARDLTEYKVDLIRENVQRAGVSCVVPKVKDAEQLDAEWIGKADVVIADL
                     PCTGYGVIGRKPDIKYYASKEKEEELVRIQRAILANAASYVKPGGTLLYSTCTVNRKE
                     NSENAAWFLEQFPFEAVSLDELLPDELKSGETAKGQLQLIPGVHKTDGFFLAKFRKTE
                     EGRA"
     gene            11372..>12320
                     /gene="rlmN"
                     /locus_tag="PLN77_04330"
     CDS             11372..>12320
                     /gene="rlmN"
                     /locus_tag="PLN77_04330"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRM43542.1"
                     /translation="MDIKSMTLDELKDACQQMGEKPFRAKQLYDWMHHRLAGSYDEMT
                     NLSLAFREKLKEQYPLTCLEKVQVQESKIDGTRKYLFRLSDGNVIESVWMKYHHGNSV
                     CISSQVGCRMGCRFCASTIGGLVRCLTPSEMLDQIYRIQEDTGERVSNLVVMGTGEPM
                     DNYDNLVRFVRLLSSQEGLNISQRNITVSTCGIVPNMYKLADEGLQITLALSLHAPND
                     EKRRELMPIANKYSIDEILDACRNYFAKTGRRITFEYSLVGGKNDSEADAKELSARIS
                     DINCHVNLIPVNPIKERDYVKSTKKVVENFKNKLEKYGIN"
BASE COUNT         3304 a         2852 c         3571 g         2593 t
ORIGIN      
        1 tgagggtcag cttggttcct taaaagatcc ggatcacgga atttacccga tggtaacttc
       61 ttcttccaca ctggccggct acggcgcagt aggagcaggt gttccgccat atgaaatcaa
      121 gaaaattgta acggtgtgca aggcttactc cagcgcagta ggagcaggtg catttgtttc
      181 cgagattttt ggagatgagg cagatgagct cagaagacgc ggcggagacg gcggagagtt
      241 cggtgcaacg acaggacgtc cgagacgtat gggatggctc gactgtgttg cttcccgcta
      301 cggctgcaga atgcagggta ccaccgatgt agcgttcacc gtacttgacg tacttggcta
      361 tctggatgag attccggtct gcacagcata cgaagtagac ggaaaagaaa tccacgattt
      421 cccgaacacc agcattcttg agaaagcaaa accggttctg aaaacccttc cgggatggaa
      481 atgcgatatc cgcggcatca agaaatacga ggatctgccg gagaactgca gaaactacat
      541 cgagtttgta gagaaagaaa tcggattccc gatcaccatg atctccaacg gaccgggaag
      601 agacgatatc atttaccgca ataaataatt taggcggttt ttagaggatt tttatgaaaa
      661 aagcaggaca gcttctcgca gttctgacag cggcttccgt gctgctgtcg ggctgtgaga
      721 agttaaaaga tgtgacaacg acatctgtct atgtgagcaa aaatggtgtt gtgacagaag
      781 ctattgtgga agattacagc aaagacgatg attacacgga agacgaactg aaggtctttg
      841 tggaggatga catcaaaaaa ttcacagagg agcgcggcga tgcggattcc gtgaagctgg
      901 aaaaatgcca gatcaaagag gacaaggtgg agatccggat ggaatacgga gattaccagt
      961 cctacgcgga ttatcacgga gcagaatttt tcgcaggcac gctggatgag gcagaagaag
     1021 cgggatatga tttttccgct tcctttgtcg attctaaagg caatgaggtt tcagtcgaag
     1081 atgcggtcaa aggcgttaag catgtacgcg tgattgtctg cgaggagccg ctggaaatcg
     1141 taacggaaga tccggtgctc tgcgtttccg gtacagccgt gatcaaagga aaaaacacgg
     1201 tggatacggc cggcgagtgg accacaaagt ccacagagag tgcttctgcg tcatcatcag
     1261 agcagactaa gacagaagaa acagagcagg aatatgagca ggacgttctt cttgccagtc
     1321 ctgttattgt tgtttatggt aagtaaacaa ataaaataga ggagtaacta actatggaaa
     1381 agacaatgga caaaattgtg gcagtagcaa aatccagagg ttttgtatat ccgggttccg
     1441 aaatctacgg cggcctggca aatacctggg attacggccc gctcggtgtt gagctgaaaa
     1501 acaacgtgaa aaaagcatgg tggcagaaat tcgtcatgga gagcccgcac aacgtaggtg
     1561 ttgactgcgc gatcctgatg aacccgcaga catgggtagc ttccggtcat ctcggcggat
     1621 tttccgatcc tctgatggac tgtaaagagt gccacgagcg tttccgtgca gacaagctga
     1681 tcgaagactt ctgtgaggaa aaaggcattg aaatcgaagg cggcagcgta gacgcatggt
     1741 cccaggaaga gatgaagaac ttcatcgagg agcatcagat tccgtgtccg tcctgcggaa
     1801 aacataactt taccgatatc cgtcagttca acctgatgtt caaaaccttc cagggtgtta
     1861 cagaggatgc gaagaacacc gtatatcttc gtccggagac tgcacagggt atcttcgtaa
     1921 acttcaaaaa cgtacagcgt acttccagaa agaaaattcc gttcggaatc ggccagatcg
     1981 gtaaatcttt ccgtaacgag attacaccgg gtaacttcac cttccgtacc cgtgagttcg
     2041 agcagatgga gctggagttc ttctgcgagc ctggcaccga ccttgactgg ttccagtact
     2101 ggagaggctt ctgcatcaac tggctgaaga gcctcggcat gaaagacgaa gagatgcgtg
     2161 caagagacca ttctccggaa gagctgtgct tctacagcaa gggaacaacc gatatcgagt
     2221 tcctgttccc gttcggatgg ggcgagctgt ggggaatcgc agaccgtacc gattacgacc
     2281 tgacccagca tcagaatgta tccggacagg atatgaccta tttcgatcca gagaagaacg
     2341 agaaatacat cccgtatgtt atcgagccgt ctcttggtgc tgaccgtgtc gttctggcat
     2401 tcctgtgcag cgcttatgac gaggaagttc tcgacgcaga gaagaatgat gttcgtactg
     2461 tgatgcattt ccatccggca ctggcaccgg taaaaatcgg tgtgcttccg ctttccaaga
     2521 agctgaacga gggcgctgag aaagtctttg cagagctgtc caagaaatac aactgcgagt
     2581 ttgatgaccg tggaaatatc ggaaaacggt atcgccgtca ggatgagatc ggtactccgt
     2641 actgcatcac ctatgacttc gattccgaga ccgatggtgc tgttaccgtc cgtgaccgtg
     2701 acaccatgga gcaggagaga attaaaattg aggatctcga tgcttacttt gcagataaat
     2761 ttactttcta atctggcttt ttagtccgga aaaagagtag aaaaaaagga ttcccactcg
     2821 agggaatcct tttttgtata ctttgtatta ctcaataatc gtacctgttt ttccctggaa
     2881 tccggcgagt gcggaatcaa tggaagtgat gactgcggta cggccgatgc ctttctcgag
     2941 gaaagaaaca gctgcttcca tcttcggcag catggagttt tccccgaact ggccttctgc
     3001 gatgtatttc ttcgcgtcag caacggtgat gtgctccagc ggtttttcgt ccggggtacc
     3061 gtagttgatg gaaacgtttt ccacgctggt caggatcatc agcatgtctg cgttcagcat
     3121 ttctgccatc aggccgctgg tgaggtcttt ttcgatgact gcggatgcac cctttaactc
     3181 ttcattctgc gggagaaccg ggattccgcc gccgcctgct gcgatgacaa cctgtccggc
     3241 atcgagaaga agacggatgg agtcgatctc tacgatatcc tgcggcttcg gagcagcaac
     3301 gattcggcgg tagccgtcct cggttttcgt tacgtagttt cctttttctt cctctgcgtc
     3361 agcctcctct ttggtaagcg tacggccgat cagtttgacc ggattgttga aggcatcgtc
     3421 gtacggatct acttcaacct gtgtaagaat cgtggatgtg gttttgaaga ttcctctgcg
     3481 gagaagctca gcacggatgg cgttctgcag gtcgtagccg atgtagcctt cgctcatagc
     3541 ggagcagaca gacatcgggg ctgcgctgta gtcggtgtgc attttgccga attcattcat
     3601 cgccatgtgg atcatgccga cctgcggcgc attgctgtgg gtgatgacaa catcagcgcc
     3661 ggcagcaacg agatctgcga ttacttttgc ggaacgtttt acggctgctt tctgttccgg
     3721 cagcgtcgtt ccaagagctt tgtgtcccag tgcaacaact attttttttc ttttcattgg
     3781 aagatacctc tctttgcagt tcatagattt tgatacgaaa aaaccaggca tcaatatagg
     3841 actattataa agtttatggg agcaaaaatc aagaagaaac cgacattgcg cacattgaaa
     3901 tgtttttcgg actatggtat tctataaaag tatctgagtc gtagggattt gaaacgtgaa
     3961 agggaggtcc cggcatgaaa aacatgcgca cgttacaaaa gcggatcgca tatcagctcc
     4021 agcagggcag tctggatgcg gaagttacag gaatcacatg cgacagcaga aaagtacagc
     4081 cgcagaacgt gtttgtctgc atccgcggaa acaggatgga cgggcacgta tatgcagaag
     4141 aagcagtcga gaggggcgct gctgttctgg ttgcggagcg ggaactggaa gggattccgg
     4201 aaaaggtaac cgtactcgta gtaaaggaca gcagagaagc actcgcacag ctttcctgtg
     4261 cctgggagga cgatccggcg cagcaccttg tgacaatcgg agtgaccggc acgaagggaa
     4321 agacgacgac tgcatatatg atttacgaaa tcctgcgaaa agcagggatc cgcgcaggac
     4381 tcatcggaac gatcgagacg aggatcgggg aaaaaagcat tccgtcagaa aatacaacgc
     4441 cggagcctgt tgtgctgcag aagctgtttt ctgaaatgat gcgggagggc tgcacccatg
     4501 ttgtgatgga ggtttcctcg caggcggaaa aacagcaccg gtgtgatgga tttacgtttg
     4561 attacggtgt gttcaccaac attggaaacg accacatcgg cgccggggag catgcatcgt
     4621 ttgcagaata tctgtcctgc aaaaagaagc tgcttcgcac ctgcagaacc ggaattgtaa
     4681 accgggatga caaaaggttt ccggagatca taaggcagag cacctgccgt ctggtgacat
     4741 tcggatgcag ggaaaaagct gattttatgg cagcagatgc agcactcgat caggcgcccg
     4801 gcgtgctcgg tgtctcatac cgggtgcagg gcgcagcgga ttttccggta cgggtagatc
     4861 tgccgggtct gttttccgtt tccaactcgc ttgccgccat tgcggtctgc gcgcagatgg
     4921 gaatcccgga aaaggcgatg caggaggcac tttccgcagt ccgcgtccgt gggcggtgtg
     4981 aggcagttcc ggtgtatccg gaaatgacgc ttctcattga ctacgcacac aatgcgatga
     5041 gcctcgaaag cctgttaaag acactgcgga aataccatcc gaagcgcctg atctgtctct
     5101 tcggctgcgg cggaaaccgc gcgcgggacc ggagattcca gatgggagag gtgtcggggc
     5161 gtctggcgga tctgaccgtc atcacctcgg acaatccgcg ggatgaggaa ccacaggcga
     5221 ttctggacga tatccggacg ggaatcggga aaacagaagg cgctttcgtt gagattgcag
     5281 accgtaagga ggcgatccgg tatgtgatcg aaaaccggca gccgggcgat ctcgtggtgc
     5341 tcgcgggaaa aggccacgaa acgtatcaga tccggaaggg gaaacggtac gagatggatg
     5401 agcgcgtgct gatccgggaa atactggaag aaagtaaggg gaatgtatga gcagcgttta
     5461 cgctgatgta atcgtagata tcacaaatga aaaactggac cggacctttc agtacaaggt
     5521 tccgccggaa ctgacagggg agatcggtcc gggaacggcg gtacagattc cgtttggaaa
     5581 cggaaaccgc acgatcaccg gctatgtgct ttccgtcgga aacgagccga aatatccgca
     5641 ggaccggatg aaggccatcc ttgcggtaca aaaggatgcg gcgactgtag agacgaagct
     5701 catcgcgctc gccgcgtgga tgcgtcatac gtacggatcg acgatgatcc aggcgttaaa
     5761 gacggtgctt ccggtcaaag taaaggcgcg cgggaaagaa acccgcgaaa ttgcgcttct
     5821 tgcagatcct gcgagatgtg aggagctgct ggaaaactac agaaagaaaa attataaagc
     5881 gagaatccgt ctcttaaacg cgctgctggc ccagccggtt cttccagggc cggttgtgag
     5941 caaggaactg aaaatcacgg cagatacgct gcacgtgatg gaagagcagg gcgtgttgga
     6001 aatccgccgt acgagcacct ggagaaatcc ggtatctgat gcggcaggaa gaacaactgc
     6061 gggaaaacca aacgaacagc agcaggccgt ggtggacggc attcgaaagg aatgggaagg
     6121 gcagaaccgc ccctgcctga tccgcggtgt gacgggaagc ggcaaaacgc tcgtctatat
     6181 ggagctgatg gagcgagtgc tgaaggaagg aaaacaggtg atcgtgctga tcccggagat
     6241 tgcgctgacg tatcagaacg tggagcggtt ctgcgcacgg tttggaacaa gggtcacggt
     6301 ggtcaactcg cggatgacac cgtccgagcg cgccgaccag atggagcgcg cgcggcgcgg
     6361 ggaggtcagt attatgatcg gtccgcgttc cgcactcttt gcaccgtttc cggatctggg
     6421 gctgatcgta atcgatgagg agcatgaaac ctcctataaa agtgaagtga cgccgcgcta
     6481 ccatgcaagg gagaccgccg tgcgccgcgc cgggctggag catgcccatg tggtgatggg
     6541 ttcggcaacg ccgtcggtgg atgcttctta cgcctgcgag acgggagcgt acgcgctgtt
     6601 ccggatggat gcgcggtacg gaaatgcagc acttccgtcc gtatggatcg cggatatgcg
     6661 ggaagaatta cagatgggaa accgttcgat tctgtccgga aaactgcggg aagagatcga
     6721 aaaacggctt gaaaaaaagg aacaggttgt actgtttctg aaccgcagag gctatgcggg
     6781 cttcgtctcg tgccgtgcat gcggccatgt gatgaaatgc ccgcactgcg atgtatcact
     6841 gacagcacac aataacggaa agctcgtctg ccattactgc ggttatgaaa caccgcaggt
     6901 acatgcgtgc ccgtcgtgcg gttccccgta catcggcggc ttccgtgccg gcacacagca
     6961 gatcgagcag aacgtgaaaa agctgtttcc aaaggcacgc gttcttagga tggacggcga
     7021 cacgacaaaa acgaaaaaca gttatgaaga aatcctgtcc tcatttgcgg cgggggaggc
     7081 cgatatcctg attggaaccc agatgatcgt aaaaggccac gatttcccga atgtcacgct
     7141 ggtgggagcg ctggcggcgg atctctccct gaatgcagtc gattaccgtg ccggggagcg
     7201 tacgttccag ctgctgacgc aggcagtcgg ccgcgccgga agaggcgaaa agccgggaat
     7261 ggcagtcatt cagacgtacc atcccgaaca ttacagcatt cagacggcgg cagagcagga
     7321 ttacgggaca ttttacgaga aagaaatgga ctaccgccgt ctgatgggat atcccccggc
     7381 ggcggagctt ctggcgatcc acggagccgg ggaggatgaa gcgcatctga cgcaggcgat
     7441 ggaatacatt cgcagatatc tcatccgcat ccggggaaac cgcgaagtcc agatcatcgg
     7501 cccggcaagt gaggcagtgt cgaaaatcaa tgacctgtac cgcatggccg tgtatgtacg
     7561 cgcgccgcag gaagaggtgc tggcagagct gcgggaaaaa ctggagcggt atatcgagat
     7621 taacaaaggc ttccgtaccg tttatttaca gtttgacttc agcagaaagt attagttttt
     7681 tcatactatt tacacataga ttatttataa tagaagagca aaagaaagga aaagaattat
     7741 ggcaatcaga gaaatcagag tcgtaggcga ccgcgtatta acgaaaaaat gcagaccggt
     7801 ggagaagatg acaccaagaa tcagcgagct gatcgacgac atgtttgaca ccatgtatga
     7861 ggcaaacggt gtcggccttg ccgcaccgca ggtcggcatc ttaaagagaa tcgtagtcat
     7921 ggatgtggat ggaaccccac atcttctgat caatccggaa atcctggaga cttccggaag
     7981 ccagacagga gcagaaggct gcttaagcct tccgggaaaa tccggtacag taacccgtcc
     8041 gaattacgta aaggtaagag cgctggaccg cgacatgcag ccgtttgaac tggagggaac
     8101 cgaacttctt gcccgctgca tctgccacga gtgcgatcac ctcgatggac atatgtatac
     8161 agaaaaagta gaaggcgaac tctgggacaa cacgtacgat gagtatgagg agtacgagga
     8221 cgaagagtaa gtcttaaaat aaaggagatg acttcagatg aaagtggtat ttatgggaac
     8281 cccggatttc tccgtgggaa cacttgaggc actcgtggag gccggttatg agatcacagg
     8341 cgttgtgacc cagccggaca aaccgaaggg ccgcggaaaa cagatgatgc cgacaccggt
     8401 gaaagaggca gccgaaaaac acggccttcc ggtctatcag ccgcgccgcg tgcgggatgc
     8461 agaggcgatc gaggagatcc ggaaaatgga gccggatgtg atcgttgtcg ttgcattcgg
     8521 ccagattatt ccaaaagaga ttttggacat gccgaaatac ggatgcatca acgttcatgc
     8581 atccctgctt ccggcttacc gcggagcagc gccgatccag tgggctgtca tgaacggcga
     8641 tgaagtgagc ggcgtaacca ttatgaagat ggatgaaggc ctcgacacag gcgatatgct
     8701 gacgaaagtg gaagttccgc ttgcagcaga tgagaccggc ggcagtctct ttgacaagct
     8761 cgccgcagcc ggagcaaaac ttctggtgga gacacttcca aaacttgaaa aaggcgaagt
     8821 gacaccggaa aaacagccgg agataagcac cacagagtat gcgcggatga tcaaaaaaga
     8881 agacggaaag atcgactgga cgaagagtgc cgttgagatc gaacgtcaga tccgtgcgat
     8941 gagcccgtgg ccgagtgctt ttacaaaagt aaacggaaag aacctgaaaa tctgggatgc
     9001 caaagtcatt gccatgttcg gcggcgatct gttctcgaaa attccgggac agaatccgac
     9061 gaaaagtgcc ggaaaagtct gggtggcaga tgagacaggc ctccatgtaa agacgggaga
     9121 cggcattctg gtgatcgagg agctgcagct cgaaggaaag aaacggatga aaaccgccga
     9181 tttcctgcgc ggctatgcga tcgagccggg taccagactt ggcgaataaa agagaaaaag
     9241 caacctgaag ttgaggcggg gaactacaca gcggtgtgat ggctgctgtg cctgagaaaa
     9301 aaggagaaaa acgatatggg acttttttat gacagtacgt atctgctggt gattcttgcc
     9361 gcactgattt ccatggcggt atccgcccgc atgaacacga ttttcagcaa atatcagacc
     9421 gtccgctcgt acagcggcat gacgggacgc gaggcggcga tgcgcattct gcatcaggcc
     9481 ggcatctacg atgtgaccgt ccgccacgta tccggtaaac tgaccgacca ctatgatccg
     9541 cggacaaaaa ccgtgaattt atcggatgcg gtttacgatt caacctcggt cgcagcgatc
     9601 ggcgtcgcag cgcatgagtg cggccatgcc gtacagcaca atacggatta cgtgccgctg
     9661 aaagtaagaa gcgccattgt tccggcagcg aatcttggat cccagctttt ctggccgctg
     9721 ttcctgatcg gactgatttt cagccttccg atgcttgtga aagccgggat ctggctgttt
     9781 gtgctggcac tggcattcca ggtggtaaca cttccggtcg agttcgatgc gtcgagaaga
     9841 gcattaaaaa tgctcagtga gggcggtatg gtgacagaga cggaacacga cggagtcaag
     9901 aaagtactct gggctgcagc catgacatac gtggcagcgg tgatcggttc tctgctgcag
     9961 ctgctccggc tgctgatttt atccggagca tttaggagaa atgatgactg atacaatcaa
    10021 tacaagagag ctgattatga acattctgct tgagatggac agggagaaaa cgccctgcca
    10081 tcttgctgta agggacgccc tcgataaata ccagttcctg ccgcggcagg accgtgcgtt
    10141 tatcaagcgg gtctgcgagg gaaccgtgga atacggcatc cagaatgact acatcatcaa
    10201 tcagtattcc aaaatcaaga tcaacaaatg caagccggtg attcgtgcga tcttacggat
    10261 gtcggtgtac cagatccgtt ttcttgacgc agtaccggat tcggcagtcg tcaatgaggc
    10321 ggtgaaactg gcggaaaaga agcattttta caacttaaag ggctttgtca acggtgttct
    10381 gcgcacgatt gtgcgggaga aggatcatct gccgataccg aaggagaaca atactacgca
    10441 gtatctttcc gtgaaatact ccatgccgga atttctggtt gaggagttcg tatcccagta
    10501 cggaaaagaa acggcagaaa cgatgatggc ggatttcctg aaagaaaaac aggtgacgat
    10561 ccgtatcaac cgctggaaca atacgatcga ggagacgaag aaaagcctgg aaagccaggg
    10621 ggcagagctg gaaaaagcgc cgtatcttgg cgaggcgttc tatctgaaaa actttgagac
    10681 gatcgcatca ctgaatgcct tccaggaagg ccggttccag attcaggatg tgagctccat
    10741 gatggcggcg catctggcgg atccaaagcc gggtgaccag gtgctggatc tctgcgcggc
    10801 accgggtgga aagagcctcc atgcagcaga taaaatgaga aatcagggat gcgtggaagc
    10861 gcgtgatctc accgaataca aggtcgatct gatccgggaa aatgtgcaga gagcaggtgt
    10921 ttcctgcgtc gtgccgaagg taaaagatgc ggaacagctc gatgcggaat ggatcggaaa
    10981 agcggacgtt gtgattgcgg acctgccgtg caccggatac ggcgtcatcg gcagaaagcc
    11041 ggatatcaaa tattacgctt caaaagaaaa agaagaggag ctggtacgga ttcagcgggc
    11101 aatccttgca aatgccgcat cgtacgtaaa gccgggcggc acactgctgt acagcacatg
    11161 taccgttaac agaaaagaaa acagcgagaa tgccgcatgg tttctggaac agttcccgtt
    11221 tgaagcagtc agtctggatg aactgctgcc ggatgagctg aaaagcggag aaacggccaa
    11281 aggacagctg cagctgatcc cgggggtaca caagacagac ggatttttcc ttgcaaaatt
    11341 ccggaaaaca gaggaaggac gggcgtaaca gatggatatt aaatcaatga ccttagacga
    11401 attaaaggac gcctgccagc agatgggaga aaagccgttc cgtgccaaac agttatacga
    11461 ctggatgcat catcgtctgg cgggcagcta cgatgagatg acgaatctct cgcttgcctt
    11521 ccgggaaaaa ttaaaggaac agtatccgct tacctgtctc gaaaaagtgc aggtgcagga
    11581 gtccaaaatc gacggaacga gaaaatacct gttccgcctt agcgacggca acgtgatcga
    11641 gagcgtctgg atgaagtacc accacggaaa ttccgtctgc atctcatccc aggtcggctg
    11701 ccggatgggc tgccgcttct gcgcgtccac aatcggcgga ctcgtgcggt gcctgactcc
    11761 gtcggagatg ctcgaccaga tttaccggat tcaggaggac accggggagc gggtctccaa
    11821 tctggttgtc atgggaacgg gtgagccgat ggacaactac gacaatctgg tgcgcttcgt
    11881 gcggctgctt tcgagccagg aaggactcaa catcagccag agaaacatca ccgtttccac
    11941 ctgcggaatc gtgccgaata tgtacaaatt ggcagatgag ggactccaga tcaccctggc
    12001 actttccctt catgcgccga acgatgaaaa gcgccgcgag ctgatgccga ttgccaataa
    12061 atattccatt gatgaaattc tcgatgcatg ccgcaattac tttgcaaaaa caggccgtcg
    12121 gattacattt gagtactctc tcgttggcgg gaaaaatgac agcgaagccg atgcaaaaga
    12181 gctttccgcg cggatttcgg atatcaactg tcatgtaaat ctgatcccgg tcaacccgat
    12241 caaagagcgc gattacgtga aatcaacgaa aaaagtcgta gagaatttca aaaataaact
    12301 tgaaaaatac ggaattaatg
//
LOCUS       DAOMUF010000048        12291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1180_length_12291_cov_4.305819, whole genome shotgun sequence.
ACCESSION   DAOMUF010000048 DAOMUF010000000
VERSION     DAOMUF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1180_length_12291_cov_4.305819"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /gene="tilS"
                     /locus_tag="PLN77_04335"
     CDS             <1..1085
                     /gene="tilS"
                     /locus_tag="PLN77_04335"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRM43543.1"
                     /translation="CAKEQGAVKIALAHHQDDVAETMLHHLARGTSLAGLASLRPVRG
                     NVIRPLLCAGRKEIRQELESRKCSWCEDSTNAEDAYTRNGIRHHVLPYLTEEVNPRTA
                     AHMAQTSLDLLETEEYLEQQTDQLMERYASAEKNAVVLRDAVSSEAPLLQRYVIRRVL
                     EQLAGKRKDLTREHLESVRELFEKQVGKSVCLPYGITAVRGYETLRLEKQGVHLKEER
                     KRKSGEEVPIPVPAGWEEEKSLAFAENPVTIVKKTSVFPERIEEKKYTKCFDCDKIKD
                     GLVLRTRRSGDYLRVTAKGGKKKLKDYMIDAKIPKEERDSILLLADGPEIWWVVGYRR
                     GESGRVGEDTKAVLQIQIGVGKEENR"
     gene            1082..1618
                     /gene="hpt"
                     /locus_tag="PLN77_04340"
     CDS             1082..1618
                     /gene="hpt"
                     /locus_tag="PLN77_04340"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRM43544.1"
                     /translation="MKETIRVLLSEEEVDRRIKEVADQINRDYAGKEVHLICVLKGGV
                     FFMCELAKRITVPVSLDFMSVSSYGDDTKSSGVVRIVKDLDQPLEGKDVLIVEDIIDS
                     GRTLYYLIDILKKRNPKSVHLCTLLDKPERRVRDVKVDYTCFNIPDEFVVGYGLDYAQ
                     KYRNLPYIGVVEFHEDEE"
     gene            1628..3490
                     /gene="ftsH"
                     /locus_tag="PLN77_04345"
     CDS             1628..3490
                     /gene="ftsH"
                     /locus_tag="PLN77_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRM43545.1"
                     /translation="MNRQRSGFGLYAAFMVIIVLAMFYITGKISAGSSPSQQKFETAV
                     KDGKVTSAQIVQNKEVPTGSVVVQEQGGDSYTVNVTDVNAAISLLEDADVTYTVQNVQ
                     RDSIFMTSVLPALLLGMILLFGMSMMNRSMGGGGGGGNKMMNFGKSNAHMSTDKDKKV
                     LFRDVAGLKEEKEDLVEVVDFLKAPEKYTQVGARIPKGVLLVGPPGTGKTLLAKAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEEGKKHAPCIIFIDEIDAVARRRGTGMG
                     GGHDEREQTLNQLLVEMDGFGVNEGIIVMAATNRVDILDPAILRPGRFDRRVVVGPPD
                     VMGREEILKVHAKNKPLAEDVDLKQIAQTTAGFTGAELENLLNEAAIMAAKDRRRFVR
                     QSDIKSAFIKVGIGAEKRSKVISDKEKRITAYHETGHAILFHMLPDMEAVYTISIIPT
                     GPGAAGYTMPQPENDDMFQTRGKMMQYITVCLGGRVAEELIFDDITTGASQDIKQATA
                     TARSMITKYGMSENLGLVAYDSDSDEVFIGRDWGHTKSYSENVAAAIDEEVRQMIEKC
                     HDQAKQIILEHSYVLHECAKQLMEKEKLSRAEFEAIFEQEAQMTGTAPEVSGEI"
     gene            3909..5999
                     /locus_tag="PLN77_04350"
     CDS             3909..5999
                     /locus_tag="PLN77_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRM43546.1"
                     /translation="MDKTRMQQYMSQMRAMFNKHALYSDESPQYQIPFEPNPGDTVKI
                     RFRTLKNNVDAVYFISGSTRAQMQVGLVRNGFDYYEIEIPVGNEPIRYFFEIQAGRVV
                     CYYNKLGVTRELQEMYSFGIIPGFHTPDWAKGAVMYQIFVDRFCNGDPSNDVLTGEYS
                     YIGEQVNKVDDWNRFPEQMDVRNFYGGDLQGVIDKMDYLQDLGVQVIYLNPVFVSPSN
                     HKYDIQDYDYIDPHFGKIVSDEGDLLWPGDKDNTRATRYIDRVTNRANLEASNELFIH
                     LVEEAHKRGMKVILDGVFNHCGSFNKWLDRERIYEAGKGYEPGAYVAQDSPYHTFFKF
                     YNEHNWPYNEFYDGWWGHDTLPKLNYEGSEKLMEDIMRIGAKWVSPPFNADGWRLDVA
                     ADLGHSPEFNHKFWKEFRRVVKEANPEAIILAEHYGDAMSWLQGDEWDTVMNYDAFME
                     PVSWFLTGMEKHSDEFNQGLLGNSESFIGAMTYHMPAFYAPSLYTAMNELDNHDHSRF
                     LTRTNHRVGRVANVGPYAAAENTNKAVLREAVVIQMTWPGAPTLYYGDEAGQVGFTDP
                     DNRRTYPWGQEDQELIRFYKEMIAVHRRFPVLAMGSLKFLYHDYNVLSYGRFNQEEQV
                     IVVINNRNERVHVEIPVWLTGINRSSVAKLTQVFATDAVGFSSEEKEIEAPAGILEMD
                     LLPLSGVVLHRCEE"
     gene            complement(6126..6211)
                     /locus_tag="PLN77_04355"
     tRNA            complement(6126..6211)
                     /locus_tag="PLN77_04355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6175..6177),aa:Leu,seq:taa)
     gene            complement(6215..6296)
                     /locus_tag="PLN77_04360"
     tRNA            complement(6215..6296)
                     /locus_tag="PLN77_04360"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6260..6262),aa:Tyr,seq:gta)
     gene            6477..7286
                     /locus_tag="PLN77_04365"
     CDS             6477..7286
                     /locus_tag="PLN77_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRM43547.1"
                     /translation="MIKIKDYVRVGSLEEAYELNQKRSACILGGMLWTKMGQRQVQTA
                     IDLSGLGLDQIEESEEEFSIGCMVTLRQMEEHEGLNAYTDGAARESVRSIVGVQFRNL
                     ATVGGSIFGRFGFSDVLTLFLALDTEVELVHAGRMPLAEFACSKRDRDVLVRLIVKKH
                     EGVTVYQSHRNSRTDFPVLTCAVRVEDGKGCAVLGARPAKAARVELPAELAEKLAAGK
                     ASAEELKAAASEISDQFTYGSNMRGSAEYRHHLGRVLLRRCMEEIQKKEEQ"
     gene            7286..7756
                     /locus_tag="PLN77_04370"
     CDS             7286..7756
                     /locus_tag="PLN77_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRM43548.1"
                     /translation="MKISMILNGKKTEAEIDADMLLIDFVRSQGCYSVKRGCETSNCG
                     LCTVFLEGKPVLSCSVLAARANGCKVDTLEGLQEEAEEFGSFIAGQGAEQCGFCNPGM
                     IMNAIALFRENPDPSEEEIKEYLAGNLCRCSGYEGQLRGILNFLAWKKQQGGAS"
     gene            7753..10092
                     /locus_tag="PLN77_04375"
     CDS             7753..10092
                     /locus_tag="PLN77_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRM43549.1"
                     /translation="MSRENHIYEPDWSGRTDQLCSVNKPVIKKDALALVTGKPVYVDD
                     LAPKDCLIVKVLRSPHANAIVKSVKKTAAERVPGIEAIYTWEDVPKQRFTMAGQTYPE
                     PSPYDRLILDQHVRYVGDPVAIIAGKDEKCVDRARKLLKVEYEVLPAILDFHQSKDNE
                     LLVHPEESWKSLCPVGADNQRNLCASAEDHNGDVEAVLAECDEVVEHTYHVRAAQQAM
                     METFRTYCFMDTYGRLNVLSSTQIVYHARRILSNALGIPKSKIRVSKPRIGGGFGAKQ
                     TVVAEIFPAFVTWKTGKPSKMIFTREESQTASTPRHEMEVTIRLGAMKDGRIRAIDLY
                     TLSNTGAYGEHGPTTVGLSGHKSIPMYGSLEAYRFAYDVVYSNVMSAGAYRGYGATQG
                     IFAVESAVSEMAARLGIDPIRIREQNMVREGQFMPAYYGETANSCALDQCVERAKEMI
                     GWDEKYPCRDMGNGKVRSVGIAMAMQGSCISNLDVGSATIKVNDDGGYTMLIAAADMG
                     TGCDTILSQMAAECLECDVDDITVVGADTDTSPYDSGSYASSTTYITGKAVEKACMTL
                     RKRICALAAERMNVPEDETEFTGTGVVHEKSGSSMTMEEIATAAMCNNGIALEATESN
                     CSPVSPPPYMAGAVEIELDKETGEVRILDYAAVVDCGTVINPNLARVQVEGGLVQGIG
                     MTLFENIQYTDKGQMINNSFMQYKVPTRLDMGKLRVEFRSSYEPTGPFGAKSIGEIVI
                     NTPAPALAHAIYNATGLWFRELPITSEQIAMGIAEKECE"
     gene            10083..10670
                     /locus_tag="PLN77_04380"
     CDS             10083..10670
                     /locus_tag="PLN77_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM43550.1"
                     /translation="MRVTILYLAAGNSRRFGENKLLYPLDGKAVYRHLLDRLAQIAGR
                     HENWELLVVTQYERILEELAPLVKAGRLQTVFSPDSEKGISYTIRAGIEAAEKQNADA
                     CACFVADQPYLKEETAERFLESMEMQKAPLGCVFCGGESGNPAWFSKPYFSELKELSG
                     DRGGKKVLKRHWESVIPFLVEAAWELKDLDRKEDL"
     gene            10798..11379
                     /locus_tag="PLN77_04385"
     CDS             10798..11379
                     /locus_tag="PLN77_04385"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRM43551.1"
                     /translation="MQLLEDRIRKDGKVREGNVLKVDSFLNHQMDVNLFREIGKEFQR
                     RFAGEEITKILTIEASGIGIACVVAEIFNVPVVFAKKTQTKNIAGDVYTTKVESFTHG
                     RVYDIIVSKEFLGKGDKVLLIDDFLANGKALEGLATLVQDSGAELVGAGVVIEKGFQI
                     GGKLLRDRGIHLESLAIVDGMDDKTGEITFRKQ"
     gene            11392..11643
                     /locus_tag="PLN77_04390"
     CDS             11392..11643
                     /locus_tag="PLN77_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43552.1"
                     /translation="MANLIARIAVLLVLAAAGMVLADRKEKIDLSVSLLLFLAAVNVT
                     SFFSLKDNRIVVVMILTVYFLLYAGAKELLRRRAEKKRK"
     gene            11640..12119
                     /locus_tag="PLN77_04395"
     CDS             11640..12119
                     /locus_tag="PLN77_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM43553.1"
                     /translation="MNANRTSGCEHPCKSAGRMFFEFSCKFKKHPGRIALQSDIAEEL
                     HMEGDAARIHQLVSLLVDNAVKYTPEGGKIHIFWRKNGKKAEFSVQNTCDTLPEGDLN
                     RLFDRFYRADASRARESGGYGIGLFVAAAIVKAHKGKITAERTRDGICFKAVFPAKG"
     gene            12079..>12291
                     /locus_tag="PLN77_04400"
     CDS             12079..>12291
                     /locus_tag="PLN77_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43554.1"
                     /translation="MASVLKQSFRQKDERKKIPEKWKNDGRVGKADTQKSRNQKLPRE
                     KKQNKRKQKKTKQKEEYMMFDCGLQNI"
BASE COUNT         3266 a         2608 c         3746 g         2671 t
ORIGIN      
        1 cgtgcgcaaa ggagcagggg gcagtgaaaa ttgcgctcgc ccatcaccag gatgacgttg
       61 cggagacgat gctgcatcac ctggcacggg gaacgtcgct ggccggactt gcctccttac
      121 gccccgtgcg ggggaatgtg atccgtccgc ttctctgtgc cggacgaaaa gagatccggc
      181 aggaactgga aagccggaag tgttcctggt gtgaggacag cacgaatgca gaggatgctt
      241 acacgaggaa cggaatccgc caccatgtgc tgccgtatct gaccgaagag gtcaatccgc
      301 gtactgccgc gcatatggca cagacatcgc tggacctgct ggaaacggag gaatatctgg
      361 agcagcagac ggatcagctg atggagcggt atgcatccgc agagaaaaat gcggttgtgc
      421 tgcgtgatgc ggtgagcagc gaagcgcctc ttctgcagcg ctacgtgatc cggcgcgttc
      481 tggaacagct ggcgggaaaa cggaaggatc ttaccaggga gcatctggaa tctgtgcggg
      541 aactttttga aaagcaggtc ggaaaaagtg tctgcctgcc gtatggaatc accgccgtgc
      601 gcggatacga gacgctccgc ctggaaaagc agggagtgca tttaaaagaa gaacggaaaa
      661 ggaaaagcgg cgaagaagtt ccaattccgg ttccggccgg gtgggaagag gaaaaaagtc
      721 tcgcatttgc agaaaatcca gtcacgattg tgaaaaaaac atcggttttt cccgaaagaa
      781 ttgaagaaaa aaagtatacg aaatgctttg actgtgataa aataaaagac ggtcttgtcc
      841 tccgcacgag aagaagcgga gattatctga gagtaaccgc aaaaggcggg aaaaagaaac
      901 taaaagatta tatgattgat gccaagatac cgaaagagga gcgggattcc attctgctgc
      961 tcgccgacgg accggagatc tggtgggtcg tggggtatcg cagaggagag agcggccgtg
     1021 tcggggaaga cacgaaagca gttctgcaga tacagattgg cgttggtaag gaggaaaaca
     1081 gatgaaagag acaatcagag tattattaag cgaagaagaa gtagacagac gaattaaaga
     1141 agtagcagac cagatcaacc gcgattacgc aggaaaagaa gtgcacctga tctgcgtttt
     1201 aaaaggcggc gtattcttta tgtgcgagct ggcaaaacgc atcacagtgc cggtaagcct
     1261 tgactttatg tccgtatcaa gttacggcga tgacaccaag tccagcggag ttgtccgtat
     1321 tgtaaaagac ctggatcagc cgctggaagg aaaagacgtt ctgatcgtgg aggatatcat
     1381 cgattccgga agaacgcttt actatctgat cgacatcctg aaaaagagaa atccgaaatc
     1441 cgtacacctg tgtactctgc tcgacaagcc ggagcgcaga gtgcgcgatg tcaaagtaga
     1501 ttatacctgc tttaatatcc cggacgagtt cgtcgtaggc tacggtctgg attacgcaca
     1561 gaaatatcgg aatcttccat acatcggagt agtggagttt cacgaagacg aggagtaaaa
     1621 agaaaatatg aacagacagc gcagtggatt tggactctac gcggcgttta tggtgattat
     1681 cgtgcttgcc atgttctaca tcaccggaaa aatcagcgca ggcagcagcc caagtcagca
     1741 gaaatttgaa accgccgtaa aagacggcaa agtgacatcc gcgcagattg tccagaacaa
     1801 agaagtgccg accggctccg tcgtggtaca ggagcagggc ggtgacagtt atacggtcaa
     1861 tgtgaccgac gtaaacgcag ctatcagcct tctggaggat gctgatgtaa cgtacaccgt
     1921 gcagaatgtc cagagagaca gcattttcat gacatccgtc ctgccggccc tgctgctcgg
     1981 tatgatcctt cttttcggca tgtcgatgat gaaccgctcc atgggcggcg gtggaggcgg
     2041 cggaaacaaa atgatgaatt ttggaaaaag caacgcccat atgtccaccg acaaagacaa
     2101 aaaagtcctg ttccgcgatg ttgcgggctt aaaagaggaa aaagaagacc ttgttgaggt
     2161 ggtcgatttc ctgaaagcac cggagaaata tacacaggtc ggtgcgagaa tcccgaaagg
     2221 agttcttctt gtgggccctc ctggaaccgg taaaaccctt ctggcgaaag ctgttgcagg
     2281 agaggcaggc gtgccgttct tcagcatttc cggatcggat ttcgtggaaa tgttcgtcgg
     2341 cgtcggcgct tcccgtgtcc gcgacctgtt tgaggaagga aaaaaacatg cgccgtgcat
     2401 catcttcatc gatgagatcg acgccgtggc aagacgaaga ggcaccggca tgggcggcgg
     2461 ccacgatgag agagagcaga cattaaacca gcttctcgtt gagatggatg gtttcggcgt
     2521 caatgagggc atcatcgtga tggcagcgac gaaccgtgtc gatatcctgg atccggcgat
     2581 tctccgtccg ggccgttttg accgccgcgt ggttgtagga ccgccggatg tcatgggccg
     2641 tgaggaaatc ctgaaagtcc atgcaaagaa caaaccgctg gccgaggatg tggatctgaa
     2701 acagatcgcg cagacgaccg caggcttcac cggcgcagag ctggagaacc tgctgaacga
     2761 ggcagcgatt atggcggcaa aggaccgcag aagatttgtg cgccagtccg atatcaagtc
     2821 tgctttcatc aaagtgggca tcggtgcgga gaagcgcagt aaagtcattt cggataaaga
     2881 aaaacggatc accgcatacc atgagacagg ccatgcgatc ctgttccata tgctgccgga
     2941 tatggaggcg gtctatacga tctccattat cccgacaggt cctggtgcag cgggctacac
     3001 gatgccgcag ccggagaacg atgatatgtt ccagacgaga ggcaaaatga tgcagtacat
     3061 caccgtatgc ctcggcggcc gtgtggcaga ggagctgatc ttcgacgata tcacaacggg
     3121 cgcatcccag gatatcaaac aggcgacggc taccgcgcgt tccatgatta cgaagtacgg
     3181 tatgtctgag aaccttggtc ttgtcgctta cgattccgac tcggatgagg tcttcatcgg
     3241 ccgcgactgg ggccatacga agagctacag cgagaatgta gccgcagcca tcgacgagga
     3301 agtccgccag atgatcgaga aatgccacga tcaggcgaaa cagattattc tggagcattc
     3361 ctatgtgctg catgagtgtg caaagcagct gatggaaaaa gagaagctga gccgtgctga
     3421 attcgaagcc atttttgagc aggaagcaca gatgacagga acagcaccgg aagtttctgg
     3481 agaaatctga tatcttttta tttgcaatag aaaacctgca gagaacaaaa aaagtttttt
     3541 gcaggttttt ttctgcgatt ttacgggcgt ttgtgcaaat taaaacagaa aatccgaaac
     3601 gccggaaaaa ttcatcgtta ttttgaataa gaatcaaatg aaaaaaaatc gcgatttgtg
     3661 cagacttttc gagggacgta tggtactata ataactgtaa aaacccccca atacattata
     3721 tagtttttgc tacaccccat aagagaaata ccttctccaa aagagccagc gtaccccgct
     3781 ggctctttta ctatataaaa atccaataaa cacaatcgtt acatgtgttc agaaaaaggt
     3841 tgtattacat ggcggtgtgg ggtaaaatat aaggaatgaa tgattgagaa agacagaggc
     3901 gttagttcat ggataaaaca agaatgcagc agtatatgtc acagatgcgg gcgatgttca
     3961 acaagcatgc gctctacagc gatgaatccc cgcagtatca gattccgttt gagccgaacc
     4021 cgggcgacac ggtgaagatc cggttccgta ccctgaaaaa taatgtagat gcggtttatt
     4081 tcatcagcgg ttccacccgt gcacagatgc aggtcggttt ggtccggaac ggttttgatt
     4141 attacgagat cgagattccg gtcggaaatg agccgatccg ctattttttt gagattcagg
     4201 caggccgcgt ggtctgttat tacaacaagc tcggtgtcac aagagagctg caggagatgt
     4261 actctttcgg cattatcccg ggcttccata cgccggactg ggcgaagggc gctgtcatgt
     4321 atcagatttt tgtggaccgc ttctgcaacg gagatccgtc caacgatgtg ctgacgggag
     4381 agtacagcta catcggtgag caggtcaaca aggtggatga ctggaaccgc ttcccggagc
     4441 agatggatgt ccgcaatttc tacggcggcg atctgcaggg cgtcattgac aagatggatt
     4501 atctgcagga tctcggtgtg caggtcattt atctgaatcc ggtgtttgtt tcgccgtcga
     4561 accataagta tgatattcag gattatgact atattgatcc gcatttcgga aagattgtct
     4621 ccgacgaagg cgatctgctc tggccgggcg acaaggacaa tacgcgggca acgcggtata
     4681 ttgaccgtgt gacgaacagg gcgaacctgg aggcgagcaa tgaactgttt atccacctgg
     4741 tggaggaggc gcacaagcgc ggcatgaagg tgattctgga cggcgttttc aaccactgtg
     4801 gttcgtttaa caagtggctg gaccgcgagc ggatttatga ggccggaaag ggctatgagc
     4861 cgggcgcgta tgtggcgcag gacagtccgt atcacacgtt ttttaagttt tataacgagc
     4921 ataactggcc gtacaatgag ttttatgacg gctggtgggg ccatgatacg ctgccgaagc
     4981 tgaactacga gggttcggag aagctgatgg aggatattat gcgcatcggc gcgaagtggg
     5041 tttctccgcc gtttaatgcg gacggctggc ggctggatgt ggccgctgac ttaggacaca
     5101 gcccggagtt taaccacaag ttctggaagg agttccggcg cgttgtgaag gaggcgaatc
     5161 cggaggcgat tattctggcg gagcactacg gcgatgcgat gagctggctg cagggcgacg
     5221 agtgggatac ggtgatgaac tatgatgcgt ttatggagcc ggtttcctgg ttcctcacgg
     5281 gtatggagaa gcacagtgat gagtttaacc aggggcttct cggaaacagt gagagtttta
     5341 tcggggcgat gacgtatcat atgccggcgt tttatgcgcc ttcgctgtat acggcgatga
     5401 atgagctgga taatcatgat cattcgcggt tcctcacgag aacgaatcat cgtgtggggc
     5461 gtgtggcgaa tgttgggccg tatgcggctg cggagaatac gaataaggcg gtgctgcgcg
     5521 aggcggttgt gattcagatg acgtggccgg gggcgccgac gctgtattat ggggatgagg
     5581 ccggccaggt cgggtttacg gatccggata accggaggac gtatccctgg ggacaggagg
     5641 atcaggagct gatccgattc tataaagaaa tgattgcggt gcatcggaga tttccggtgc
     5701 tggcgatggg atcgctgaaa tttttgtacc atgattataa tgtgctttct tatggacggt
     5761 ttaatcagga ggagcaggta attgtggtga ttaataaccg gaatgagagg gtgcatgtgg
     5821 agattccggt gtggcttacc gggattaacc ggagttcggt ggcgaagctg acgcaggtgt
     5881 ttgcgacgga tgcggtggga ttttcttcgg aggagaaaga aatcgaggca ccggcgggca
     5941 tactggagat ggatttgctg ccgttatcgg gagtggtact gcatagatgc gaggagtgac
     6001 ggacgcctcg ccgccgcatc acgcaagacc gaacgggcgg cggcgaggct ggtagtgggg
     6061 acgggaggtt gaaaaataga aatggaaaaa gagctccgat tcgtatgaat cagagctctt
     6121 aatcgtgccg caaaccggga tcgaaccggt acgggtatca ctacccacgg gattttaagt
     6181 cccgggcgtc tgccagttcc gccactgcgg catatggacg gagatggatt cgaaccatcg
     6241 aagcaatttg cagcagattt acagtctgtc ccctttggcc actcgggaat ccgtccatgt
     6301 attttgtttt gtgtcagaat gacttccgac attgaatgat tatactggat gtggagggaa
     6361 aatgcaaggg tttttttgaa aaaaatagaa aaaagttgaa aggggggagc aggtcgggta
     6421 taatatcaaa tatatgggct tctatattga gtaaaaatgg aggatatagg gtaagtatga
     6481 ttaagattaa ggattatgtc cgggtggggt cgctggagga ggcgtatgag ctgaatcaga
     6541 agagaagcgc ctgtatcctg ggcgggatgc tctggacgaa gatggggcag aggcaggtgc
     6601 agacggcgat tgatttgtcc gggctgggac tggatcagat tgaagagtcc gaggaggagt
     6661 tttcgattgg atgtatggtg acgctgcggc agatggagga gcatgagggg ctgaatgctt
     6721 ataccgacgg tgcggcacgg gagagtgtgc ggagtattgt cggggtgcag ttccggaatc
     6781 tggctacggt gggaggcagt attttcggca gatttggatt ttcggatgtg ctgacgctgt
     6841 ttctggcgct ggatacggag gtggagctgg ttcatgcggg acggatgccg ctggcggagt
     6901 ttgcctgttc aaagagagac agggatgtgc tggtaaggct gattgtgaag aagcatgagg
     6961 gtgttacggt ttatcagagt catcgaaatt ccagaacgga tttcccggtg ctgacgtgtg
     7021 cggtgcgggt ggaagacgga aaaggatgtg cggtgcttgg ggcaagaccg gcgaaggcgg
     7081 cacgtgtaga gcttccggcg gagctggcgg aaaagctggc ggctggaaag gcatctgcgg
     7141 aggagctgaa ggcggcggct tcggagattt ctgatcagtt tacgtatggc agtaatatgc
     7201 gcggaagtgc agagtatcgt catcatctgg gacgggtgct gctgcgcaga tgtatggaag
     7261 aaattcagaa gaaggaggag cagtgatgaa gatttcgatg atcttaaatg gaaagaagac
     7321 ggaagcggaa attgatgcag acatgctcct gatcgatttt gtgagaagcc agggatgcta
     7381 cagtgtgaag cgcggctgtg agacgtcgaa ctgcgggctg tgtacggttt ttctggaggg
     7441 aaaaccggtg ctgtcgtgtt cggtgcttgc ggcgcgggcg aatggatgta aggtggatac
     7501 gctggaaggc ctgcaggagg aagcagaaga atttggttcc tttattgccg gacagggcgc
     7561 cgagcagtgc gggttctgca atccggggat gattatgaat gcgattgcgc tgttccggga
     7621 aaatccggat ccgagtgagg aagaaattaa agaatatctg gcgggaaatc tctgccgctg
     7681 ttccggatat gaaggacagc ttcggggaat tttgaatttc cttgcctgga aaaagcagca
     7741 gggaggtgcg tcatgagcag agaaaatcat atctatgaac cggactggtc gggaagaacc
     7801 gatcagctct gcagtgtgaa taagccggtg atcaaaaagg atgccctcgc gctggtaacc
     7861 ggaaagccgg tttatgtaga tgatctggcg ccgaaggatt gtctgattgt caaggtactg
     7921 cggagtccgc atgccaatgc aatcgtaaaa tccgtgaaaa agacggcagc agagcgtgtt
     7981 ccgggcattg aggcgattta tacctgggag gatgtgccga aacagcgttt tacaatggca
     8041 ggacagacct acccggaacc aagcccgtat gaccgtctga tcctcgatca gcatgtgcgc
     8101 tacgtgggag atccggtggc gattattgcc ggaaaagatg aaaaatgcgt ggaccgtgcg
     8161 cggaagctcc tgaaagtaga gtacgaagtg cttccggcca ttctggattt tcatcagtcc
     8221 aaggataatg aactgctggt gcatccggag gaaagctgga aatcgctctg tccggtcggt
     8281 gcggacaatc agagaaatct ctgtgcaagt gcggaagatc acaacggaga tgtggaagcc
     8341 gtgctcgcag agtgcgatga ggtcgtagag catacgtatc atgtacgcgc agcccagcag
     8401 gcgatgatgg agacgttccg gacatactgc tttatggata cctacggacg gctgaatgtg
     8461 ctgagctcga cccagatcgt ttaccatgcg cgccgcattc tgtccaatgc actcggcatt
     8521 ccgaaatcga agatccgtgt ttccaagccg agaatcggcg gcggattcgg agcgaagcag
     8581 acggtggttg ccgaaatttt cccggcgttt gtaacctgga aaaccggaaa accgtcgaaa
     8641 atgatcttta cgagagaaga gtcccagaca gcatccacac cgcgccatga gatggaagta
     8701 acgatccgcc tcggtgcgat gaaggacgga agaatccgtg cgatcgatct gtatacactt
     8761 tccaacacgg gtgcatacgg cgagcatgga ccgacaacgg tcggcctttc cggccacaaa
     8821 tcgattccga tgtatggatc tcttgaggct taccggtttg catatgatgt tgtgtattcc
     8881 aacgtaatgt cagccggtgc ttaccgcgga tacggtgcga cgcagggtat ctttgccgta
     8941 gaatctgctg tcagtgagat ggcagcgcgt cttggcatcg atccgatccg gatccgggag
     9001 cagaacatgg tccgtgaggg acagttcatg ccggcttact atggcgaaac ggcaaacagc
     9061 tgtgcgcttg accagtgcgt ggagcgtgcg aaagagatga tcggctggga tgagaaatat
     9121 ccgtgccgtg atatgggaaa tggaaaggtg cgcagcgtcg gcattgcgat ggcgatgcag
     9181 ggctcctgta tttccaatct cgatgtcgga tcggcaacga tcaaagtcaa cgacgacggc
     9241 ggctatacga tgctgatcgc agcggccgat atgggaaccg gctgcgatac gattctgtcc
     9301 cagatggcgg cagagtgtct ggagtgtgat gtggatgaca ttaccgttgt cggcgcagat
     9361 accgatacgt ccccgtatga ctccggttcc tatgcatcga gtacgacgta tattaccgga
     9421 aaagcggtgg aaaaagcctg catgacgctt cggaaacgaa tctgtgccct ggcggcagag
     9481 cggatgaacg tgccggaaga tgagacagaa tttaccggta ccggcgtcgt gcatgaaaag
     9541 agcggaagca gcatgacgat ggaagaaatc gcaacagcgg ccatgtgcaa taacggcatc
     9601 gcgctggaag cgacggaaag caactgctct ccggtatcgc cgccgcctta tatggctggt
     9661 gcggtcgaga tcgagctgga taaagagacc ggcgaggtca ggattctcga ttatgccgcc
     9721 gttgtcgact gcggaaccgt catcaacccg aaccttgcgc gcgtacaggt cgagggcggt
     9781 ctggtgcagg gcatcggcat gacgctgttt gaaaatatcc agtacacgga caagggtcag
     9841 atgatcaata attcgtttat gcagtataaa gtgccgaccc gcctcgatat gggcaagctg
     9901 cgcgtcgagt ttcggagcag ctacgagccg accggtccgt tcggtgcaaa atcgatcggc
     9961 gagatcgtca tcaacacacc ggctccggcg ctggcacatg caatctacaa tgcgacgggt
    10021 ctgtggttcc gcgaactgcc gatcacgagc gagcagattg caatgggaat tgcggaaaaa
    10081 gaatgcgagt aacgatactt tatctggcag ccgggaacag cagacggttc ggggaaaata
    10141 agctgctcta tccgctggat ggaaaggcgg tgtaccgcca tctgctggac cggctggccc
    10201 aaattgccgg aaggcatgag aactgggaac tgctggttgt gacgcagtat gaaaggattc
    10261 tggaggagct tgcgccgctg gtaaaagccg ggcgtctgca gacggtgttt tcgccggaca
    10321 gcgaaaaagg aatttcctat acaatccgcg cgggaatcga ggcggcggag aagcaaaatg
    10381 ccgatgcctg tgcgtgtttt gtggcggatc agccgtacct gaaggaagag acggcggagc
    10441 gctttctgga atccatggag atgcagaagg ctccacttgg ctgcgttttc tgcggcggcg
    10501 agagcggcaa tccggcgtgg ttctcaaaac cttatttttc agaactaaaa gaactttccg
    10561 gcgaccgcgg cgggaaaaag gtgctgaaac ggcactggga atcggtaatt ccgttcctgg
    10621 tcgaagccgc gtgggaatta aaggatctgg accggaaaga ggatctctga tgcagagaca
    10681 ccggcggctg ccatgaataa aatttgagac agtgcgaaat ttgatggatt tggacttgca
    10741 tttccagtga gaagtaagta caatgggtat aataaaaatt cgggaggctt caaaaaaatg
    10801 cagttattgg aagacagaat ccgcaaagac ggaaaagtac gcgagggaaa tgtcctgaag
    10861 gtggacagct ttttaaatca tcagatggac gtcaatctgt tccgggaaat cggaaaagag
    10921 tttcagcgtc gttttgcggg agaggagatc acgaaaatcc tgaccatcga ggcatctgga
    10981 atcggaattg cctgtgttgt ggctgaaatt tttaatgttc cggttgtgtt tgcaaagaaa
    11041 acacagacca aaaatatcgc cggcgatgtt tataccacaa aagtagaatc tttcacccat
    11101 ggaagagttt atgatatcat tgtttccaaa gaatttctgg gaaaaggaga caaagttctc
    11161 ctgatcgatg atttcctggc aaacggaaaa gcgctggaag gactggcaac cctggtacag
    11221 gattccggtg cagagctcgt aggagcaggc gttgtcatcg aaaaagggtt ccagatcgga
    11281 ggaaaactgc tgcgtgaccg cggcattcat ctggaatctc ttgccatcgt cgatggtatg
    11341 gatgataaaa ccggggaaat taccttccgg aaacagtaag gaggcgtcga aatggcaaat
    11401 ctgattgcaa gaattgccgt tcttctggtt ctggcggccg caggaatggt gctggccgac
    11461 agaaaagaaa aaattgatct cagtgtgtca ctgctgctgt ttctggcggc agtcaatgtg
    11521 acctcttttt tcagcctgaa agataacagg attgtagttg ttatgattct gacggtatat
    11581 tttctgttgt atgcaggcgc aaaagagctg ctgcggagac gggcagagaa aaaaagaaaa
    11641 tgaatgcaaa ccgaacatcc ggctgcgagc acccatgcaa aagtgccggt cggatgtttt
    11701 ttgaattttc ctgtaaattc aaaaaacatc cgggcaggat cgcgctgcag agcgatattg
    11761 cagaagaact ccatatggaa ggggatgccg caagaatcca ccagctcgtt tctcttcttg
    11821 tggataacgc cgttaaatac actccggagg gtggaaaaat ccacattttc tggcgaaaaa
    11881 acggcaaaaa agcagaattt tcggtgcaga atacctgcga taccctgccg gaaggtgatc
    11941 tcaaccgcct ctttgaccgc ttctaccgtg cggatgcgtc gcgggccagg gaaagcggcg
    12001 gctatggaat cggccttttc gtggcagctg caattgtaaa ggcacataag ggaaaaatca
    12061 ccgcagagcg gacacgagat ggcatctgtt ttaaagcagt ctttccggca aaaggatgaa
    12121 agaaagaaaa ttccggaaaa atggaaaaat gacggcaggg taggaaaggc agacacacaa
    12181 aaaagcagaa atcaaaagct tccaagagag aaaaaacaaa acaaaagaaa acaaaagaaa
    12241 acaaaacaaa aggaggaata tatgatgttt gattgtggtt tacagaatat t
//
LOCUS       DAOMUF010000049        12238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1188_length_12238_cov_3.492982, whole genome shotgun sequence.
ACCESSION   DAOMUF010000049 DAOMUF010000000
VERSION     DAOMUF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 12238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12238
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1188_length_12238_cov_3.492982"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLN77_04405"
     CDS             <1..229
                     /locus_tag="PLN77_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43555.1"
                     /translation="TFEIPGNTMEKWEVISGGKAELTEENGTFSISVPIPERDSIDTI
                     LKLTFAEPIVWEGCKGRENDVYGLADGLKEE"
     gene            complement(312..1565)
                     /locus_tag="PLN77_04410"
     CDS             complement(312..1565)
                     /locus_tag="PLN77_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM43556.1"
                     /translation="MNSDIVSLIIILICVMLSAYFSATETSFTSLNRIRMKNMAEKGD
                     KRAELVLKLLENFDKLLSTILVGNNIVNIGLSSLATVLFIKLLGGDSGATVSTIITTI
                     VVLIFGEVSPKSIAKESPETIAKFSAPFLHILMIMLTPVTFLFSQWKKLLSRIFKSSE
                     NHGITDEELMTIVEEATQEGDIDDQEGDLIQNAIGFMEMEAAEIFTPRVNVIGIPLDA
                     TKSEIAKTFSDTGFSRLPVYDDDIDHIVGIVYQKDFHNHIYHTNKPLSSIIRPALFVT
                     ENTKVGPLLKKMQAKKSHIAIIIDEFGGTDGIITMEDILEELVGEIWDEHDAVVQEIQ
                     KVSENEYLVTGTASIDKVYEELDIDKEFDVFTVSGWIMGILERVPKEGDHFVSDGLDV
                     TVLKMADRRVDKVRIVRLPKDENEQ"
     gene            complement(1804..3198)
                     /gene="uxaC"
                     /locus_tag="PLN77_04415"
     CDS             complement(1804..3198)
                     /gene="uxaC"
                     /locus_tag="PLN77_04415"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HRM43557.1"
                     /translation="MKPFMDADFLLQTDTAKTLYHEAAEKMPIFDYHNHLNPQEILED
                     RQMENLTRVWLGGDHYKWRAMRAMGFSEDLITGNADDYDKYLAFAETIENAIGNPLYH
                     WTHLELQRYFGITTPLSRATAKEIWDEANAKLQTKEFTVRNLILNQHVSHLCTTDDPA
                     DDLHCHLALQKEDFACRVLPSFRPDRAVHLEKPDFPEYVEKLAAAAGRTIASAEDMVH
                     ALSCRLDFFLSAGCVVSDHSLEGCFYVPCTAAEANDVFEKRMNGGVLTEKELGMYKGF
                     LLTNLGRLYHKHNIAMQLHIKALRNNSKRMFRALGADTGFDSMNDFAFAPMLGTFLND
                     MDEDDALPKTVLYSLNPGDNPMLASMAGNFAAPGIRSKVQFGTAWWFNDHKYGMESQL
                     ATLSSIGMLSTFIGMLTDSRSFLSFPRHEYFRRILCNQIGNWVENGEYPANLEFLKEM
                     VENISYNNAVSYFL"
     gene            complement(3216..4850)
                     /locus_tag="PLN77_04420"
     CDS             complement(3216..4850)
                     /locus_tag="PLN77_04420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HRM43558.1"
                     /translation="MSLNLTAAGLADQKAWEAAGYALPSYDREAMITRTKESPCWVHF
                     GAGNIFRAFQANTAQELLNNGTFDRGVIVAEGFDTEIIRDMYQPHDNLSILVTLKADG
                     SVEKTVVGSIAESLAADTADSPDFARLKEIFTKDSLQMATFTITEKGYSLKNGAGELL
                     PSVAADFAAGPSSVTSYMGKVASLLYERFLAGEKPVAMVSTDNCSHNGEKLSLALTAY
                     ASAWEENKLVQPGFLSYLQNPEKVSFPWTMIDKITPRPDGSIEKILEENGLADAQPIV
                     TSRHTYVAPFVNAEECQYLVVEDHFPNGRPPMEKSGWIFTDRETVNKTERMKVCTCLN
                     PLHTALAVFGCLLDYELISEEMKNPVLKKLVERIGYIEGLPVVTDPGILSPKQFIDEV
                     LNIRIPNPFMPDTPQRIATDTSQKLSIRFGETIKSYLASPELSLSDLQAIPAVFAGWL
                     RYLMGVDDNGDAFDLSPDPLLATVRPYVQDLKLGAPADRETLSKTLAPLLSDASIFGV
                     DLISAGLSDRVLDAFVSMLHGPGAVADTLAALTAQF"
     gene            complement(4866..5942)
                     /gene="uxuA"
                     /locus_tag="PLN77_04425"
     CDS             complement(4866..5942)
                     /gene="uxuA"
                     /locus_tag="PLN77_04425"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HRM43559.1"
                     /translation="MEMTFRWYGSKFDTVTLEQIRQIPGVTGVITTLYDTTPGEVWTR
                     EAIHALKEEVEASGLHISGIESVNIHDAIKIGSPDREQYIDNYIETLKHLGEEDIHLV
                     CYNFMPVFDWTRTELARVRPDGSTVLAYKQSAVDALVPEKMFESIAGDANGAILPGWE
                     PERMAKVKELFDAYRDVDDEKLFANLKYFLERIMPVCNEYDIKMAIHPDDPAWSVFGL
                     PRIIINKENILRMMKMVDDPHNGVTFCSGSYGTNLENDLPDMIRSLKGRIHFAHVRNL
                     KFHSQQDFEEAAHLSSDGSFDMYEIMKALYDIGFDSPIRPDHGRMIWGEKAMPGYGLY
                     DRALGATYLNGLWEAIDKSHKRGI"
     gene            6693..7931
                     /locus_tag="PLN77_04430"
     CDS             6693..7931
                     /locus_tag="PLN77_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRM43560.1"
                     /translation="MSGCGCSFTPVENKETEEIKYTDALAEQFAAEVGVDPRPNETLV
                     EIDERGAFIRQPNAFIQPFGDKEGDLKAEANRFGIYWATGCNWSNRPIIVRELLGLQD
                     VISETRVSPSGETNRYGHAFGQYPDFKDPATGAYFLSEFYKRANPDFKGRATTPTLVD
                     VKEKKAVNNDYHRLTNYLEVQFRPFQPKDAPDLYPKKFRKEIDEFNDWLFPHVNNGHY
                     RMAFCQSPEAYDEAYEDFYESLDKLDKRLETNRFLFGDYITDSDVRAYVTLIRWDVSY
                     FHNVGPVKKPIRDYKNIWGYLKELYAIPAFRHGSDPQVLALSRPGKKLGEVLFRGYNE
                     RILAKVDFEKLMADDGSRRTLSKTPDEVFLRHPEGETYEDYAGEISKTIWNSPKWEDR
                     NPKNGVLSVDASINPLKGLL"
     gene            8030..8953
                     /locus_tag="PLN77_04435"
     CDS             8030..8953
                     /locus_tag="PLN77_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM43561.1"
                     /translation="MKKTKKFWAVLAAAGLLAASFAGCGSNASANAGSSSGSAASSSA
                     EGSSASDSGEKKVIKAVTSGTLAPYFYVNDDNELTGVDIDIVKEVFNRLPQYELKIEQ
                     ADALQGVLSGQYDIAVNNYGYTDERAESYYFSLPYKTSFNEYIQRTGDEPLTSLTDLA
                     DRGYKIELSAGSLTANALEKWNTDNPDHQINIVYSNANFQVRYQHIVDGTADVAIDDG
                     PIIDNLLPQFGLEGQLEANEIDEETEDFLFPQNNTYFLFGKNEEGAALREDVDKALKE
                     MKEDGTLSKITTEYLGVDTSPDEKYFDETPN"
     gene            9049..9549
                     /locus_tag="PLN77_04440"
     CDS             9049..9549
                     /locus_tag="PLN77_04440"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM43562.1"
                     /translation="MAEIYRVEKGSEEWKYKAYDYVRTDAFCLGQNIPIELEFGQDES
                     KDDLQAILLTEDHKPVAGCRIAFPAEKTGKIERVCVVREKQKSGYGRVLIEAAEKWLK
                     EYGVDHIVITSQDRAAGFYEKLGYKLNPDADLSVYDHHVPKKDEEPKKPKKNLGFTCV
                     LVEKYV"
     gene            9565..10317
                     /locus_tag="PLN77_04445"
     CDS             9565..10317
                     /locus_tag="PLN77_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM43563.1"
                     /translation="MGKLFDVSLVFSSIPELLTKLPITLELAVVSMLIGLGLGLVMAI
                     IKMKKIRVLTQITNLLISLLRGTPVIVQLYVAFFGIPMMFKAINQQFGTNLAVANISG
                     FVYAMIALGLNQSGFMAEIIRSALQSVDPGQIEAAHALGMTYPQTLRRIILPEAFEVA
                     LPTLGNSFISIIKGTSLAFTCAVVEITAQGQIIGGRTYRYFEVYVSLAIIYWLVTLVI
                     EQIIRVIENKIRIPDQVELASANGEGEKNDKN"
     gene            10304..11062
                     /locus_tag="PLN77_04450"
     CDS             10304..11062
                     /locus_tag="PLN77_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM43564.1"
                     /translation="MIKIDHLSKKYNDNVVLDDISLEIKQGDVVGIIGPSGTGKSTLL
                     RCVNRLETPEKGTVTIGDKVIGLSERKPKELLYLRQNTGMVFQRFNLFEKKTALENVM
                     EGLIVVKKMKKDEARAIALEELKLVGMEKWANHYPKHMSGGQQQRVALARALAMKPQI
                     LLLDEPTSALDPELVGEVLDVIKKVAAQGYTMLLVSHEMNFIRHVSNRVIFLDKGHVV
                     EDGTPKEVFEHPQNQRTREFFSKMHIMAQPEYVI"
     gene            11220..>12238
                     /locus_tag="PLN77_04455"
     CDS             11220..>12238
                     /locus_tag="PLN77_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM43565.1"
                     /translation="MIDEKRIADFFAELVSIDSPSLEECEMADTLKAKFAEIGVNFTE
                     DHTQEQTGSNAGNLFARIPGSIDGAPVLFAAHMDTVEPAKGKKAVFHDDGTVTSDGTT
                     VLGADDLAGVTAIYEAVRHITEAGAPHSPIEILISTGEELYCKGANAFDYSQVQAKEA
                     YVLDLSGAIGAAAYAAPTLVSFKAEIQGKAAHAGFHPEDGINSIQAAARAVAQLPQGH
                     VAESTTANIGKIGGGSGTNIVSESCLVEGEIRSLNHETAMELLATYQKTFQQEADAIG
                     AKLVWEEKLNIHAYETPLTGVAAKHYESAVAKEGLTAAFEKTFGGSDNNVFAQHGIEG
                     LVIATS"
BASE COUNT         3285 a         2949 c         3235 g         2769 t
ORIGIN      
        1 cacgtttgaa attccgggga atacaatgga aaaatgggaa gtgatttccg gcggaaaagc
       61 agaactgacg gaagaaaacg gaaccttttc gatctccgtc ccgattccgg agcgcgacag
      121 cattgatacc attctgaaac ttacctttgc agaaccgatt gtatgggagg gctgcaaagg
      181 acgggaaaat gatgtgtatg gacttgcgga cggtctgaaa gaagaataga ggatacaaaa
      241 aaacggatcg agtatctgac tttaaaatca gatgttcgat ccgttttttg cgtgtaaatc
      301 gtatcaatcg cttactgttc attttcatcc ttcggcagcc gcacgatccg taccttgtcg
      361 actctccggt ctgccatttt cagtacggtt acgtcgaggc catccgagac gaaatgatcg
      421 ccttcctttg gcacgcgctc gaggatcccc atgatccatc cgctgaccgt aaagacgtca
      481 aattctttgt caatatcgag ctcttcatac accttgtcga tgcttgccgt tccggtcacg
      541 aggtattcgt tttcggaaac tttctggatt tcctgtacaa ccgcatcatg ctcatcccag
      601 atttcgccga cgagctcttc gaggatatct tccatggtga tgataccgtc ggtgccgccg
      661 aattcatcaa tgataatggc aatatgcgat ttcttcgcct gcattttttt cagcagcggg
      721 ccgaccttcg tattctcggt tacaaagagc gccgggcgga tgatgctgct tagcggtttg
      781 ttggtgtggt aaatgtggtt gtggaaatcc ttctgataca caattcccac aatgtggtcg
      841 atatcatcat catacaccgg cagccgggaa aatccggtgt cggagaaagt cttagcgatc
      901 tcggatttcg tcgcatcgag cggaatgccg atgacgttga cacgcggagt aaaaatctct
      961 gcggcctcca tctccatgaa gccgattgca ttctgaatca gatcgccttc ctggtcgtcg
     1021 atgtcgccct cctgggtcgc ctcctcgacg atggtcatca gttcctcatc ggtgatgccg
     1081 tggttttccg aggatttgaa gatgcgggag agcagcttct tccactggga aaagagaaac
     1141 gtcaccgggg tcagcatgat catcagaatg tggagaaacg gtgcggaaaa tttggcgatc
     1201 gtctccgggg attcttttgc aatgcttttc ggggaaactt cgccgaagat caggaccacg
     1261 atagtggtta tgatcgtgga aaccgtcgca ccggagtcgc cgccgagaag cttgataaaa
     1321 agcaccgtgg caagggagct gagtccgatg ttgacgatgt tgttgccgac gagaatcgtg
     1381 gagagcagct tgtcaaagtt ctccagcaac tttaacacca gttccgcccg cttgtcgcct
     1441 ttttctgcca tatttttcat gcggatgcgg ttcagcgagg tgaaagaagt ctccgtcgcc
     1501 gaaaagtagg ctgagagcat gacgcagata agaatgatga tgagtgacac aatgtcgctg
     1561 ttcatttgtt tttcctttct taggaaaccg ggacacgatg caaaaaatgt acaaaaagac
     1621 ataacctatc ttgaatctgg cggaatcaaa gatagattat gtctccgttt atcaaatgat
     1681 gaaaataaag catcgtcgag gcatactgcc tgagtcgtcc atgtttctgt aatcctttcg
     1741 ttgatcagta atccctttta ttgtacggta tgtgggaata attgtcaata aggcatgcga
     1801 aaatcacagg aaatacgaaa ccgcattgtt gtagctgata ttttccacca tctctttcag
     1861 aaactccagg ttcgccgggt actccccgtt ttccacccag tttccgattt gattgcagag
     1921 aatccggcgg aaatattcgt ggcgcgggaa ggagaggaag ctgcgggagt cggtcagcat
     1981 gccgatgaag gtggagagca tgccgatgct ggaaagcgtc gcaagctggg attccatgcc
     2041 gtatttgtga tcgttgaacc accatgcggt accgaactgc acttttgagc ggatgccagg
     2101 cgcagcaaag tttccggcca tgcttgcaag catcgggttg tcgcccgggt tcagggagta
     2161 gagcacggtt ttcggcagtg cgtcgtcctc gtccatatcg tttaagaaag tgccgagcat
     2221 aggggcaaag gcgaaatcgt tcatgctgtc aaagccggta tcggcaccga gcgcacggaa
     2281 catccgcttc gagttgttgc ggagtgcttt gatatgaagc tgcatggcga tattgtgctt
     2341 atgatacagc cgtccgaggt tcgtcagaag gaagcccttg tacatgccga gctctttttc
     2401 ggtgagaaca ccgccgttca tgcgcttctc aaatacatca ttcgcctcag cagccgtgca
     2461 tggcacatag aagcagccct caaggctgtg gtcgctgaca acgcagccgg cggagaggaa
     2521 gaagtcgagg cggcaggaga gggcatgcac catatcctcc gcggatgcaa tcgtcctgcc
     2581 ggcggcagcg gcaagctttt ccacatattc cgggaaatcc ggcttctcga gatgaacggc
     2641 gcggtccggg cggaaggacg gcagaacgcg gcaggcaaag tcttccttct gtaatgccag
     2701 atggcagtgc agatcatctg caggatcgtc ggtggtgcac agatgcgaca catgctggtt
     2761 cagaatcaga ttgcgcacgg taaactcctt cgtctgcagt tttgcattcg cctcatccca
     2821 gatctccttc gctgttgcgc gggagagcgg tgtcgtgatg ccgaagtacc gctgcagctc
     2881 cagatgtgtc cagtggtata aaggattgcc gatcgcgttc tcaatcgtct cggcaaacgc
     2941 cagatatttg tcgtaatcat cggcgtttcc ggtgatcaga tcctccgaaa agcccatcgc
     3001 acgcatggca cgccatttat aatgatcccc gccgagccag acgcgtgtca gattctccat
     3061 ctggcggtct tcgagaatct cctgtggatt cagatggttg tgatagtcaa aaatgggcat
     3121 tttttcagcc gcctcgtggt acagtgtttt cgccgtatcg gtctgcagca gaaagtcagc
     3181 gtccataaaa ggtttcatat ggaaatacct cccttttaga attgagcagt cagcgcagca
     3241 agcgtatctg ccacagcgcc aggtccatga agcatcgaga caaaagcatc cagtacacgg
     3301 tcagaaagtc cggcagaaat cagatccaca ccgaaaatcg aagcatcgga gagcagcgga
     3361 gccagcgttt tggaaagggt ctcgcggtca gccggtgcgc cgagtttcag atcctgcaca
     3421 tacgggcgga ccgtggcaag cagcggatcc gggctcagat caaatgcatc gccgttgtca
     3481 tccacgccca tcagataccg cagccaccca gcaaatacag ccggaattgc ctgaagatcc
     3541 gagagtgaca gctccggaga agcaagatac gatttgatcg tctcaccgaa ccggatcgac
     3601 agcttctgtg acgtgtcggt cgcaattcgc tgcggcgtat ccggcataaa cggattcggg
     3661 atgcggatgt tcagcacttc atcgataaac tgcttcggag agagaatacc aggatcggtg
     3721 acaaccggaa gtccttcgat atatccgatg cgctccacga gctttttcag taccggattt
     3781 ttcatctcct cactgatcag ttcatagtcc agcaggcagc cgaacacagc gagtgcggtg
     3841 tgcagcgggt tcaggcaggt gcagaccttc atgcgctctg ttttgttgac agtttcccgg
     3901 tcggtaaaga tccatccgct cttttccatc ggcggccgtc cgttcgggaa atgatcttcg
     3961 actacgaggt actggcattc ctctgcattg acgaacggag caacgtaggt gtggcgggag
     4021 gtgacgatcg gctgtgcatc ggcgaggccg ttctcctcaa gtatcttttc gatggaacca
     4081 tccggccgcg gggtgatctt gtcgatcatg gtccacggga agctgacttt ttccgggttc
     4141 tgcagatagg agaggaagcc tggctgtacc agcttgtttt cttcccatgc agaagcgtag
     4201 gctgtcaggg caagggaaag tttttcgcca ttgtgggagc agttgtcggt gctgaccatc
     4261 gcaaccggtt tttcgcctgc aagaaatctt tcatataaaa gagaagcaac ttttcccata
     4321 tagctggtaa cggaagaagg tccggcagca aaatcggcag caacagacgg cagcagttcg
     4381 cccgcaccgt ttttcaggga atatcccttt tcggtgatcg taaacgtcgc catctgcagg
     4441 gaatccttgg taaagatttc ttttaaccgg gcaaaatccg gagaatccgc cgtatcggca
     4501 gccagcgatt ctgcgatgct tccaacaacg gttttctcga cagaaccatc ggcttttaag
     4561 gtcaccagaa tgcttaaatt atcgtgcggc tggtacatat cacggatgat ttctgtatcg
     4621 aagccctccg caacgatgac accgcggtca aaggtaccgt tgttcagcag ttcctgtgct
     4681 gtatttgcct ggaaggcacg gaaaatattt ccggctccga aatgcaccca gcacggagat
     4741 tctttcgtgc gggtgatcat tgcttcgcgg tcataggatg gaagcgcata gcctgcggct
     4801 tcccacgctt tttgatctgc cagtccggca gctgttaagt taagagacat cagtaaaaac
     4861 ctcctttaaa ttccacgttt gtgactcttg tcaatagctt cccagagtcc gtttaaataa
     4921 gtcgcgccga gtgcgcggtc atacagtcca tagcccggca ttgccttctc gccccagatc
     4981 atacgtccgt ggtccgggcg gatcgggctg tcaaagccga tgtcgtacag cgctttcatg
     5041 atctcataca tatcgaagct gccgtcagag gaaaggtgtg cggcctcctc gaaatcctgc
     5101 tggctgtgga atttcagatt gcggacgtga gcgaagtgga tgcggccctt taaggaacgg
     5161 atcatatccg gcagatcgtt ctccaggttc gttccgtagg agcccgagca gaaggtcacg
     5221 ccgttgtgcg ggtcatcgac catcttcatc atgcgcagaa tgttttcttt attaatgatg
     5281 attctcggaa gaccgaagac actccatgcc gggtcgtccg ggtgaatggc cattttgatg
     5341 tcgtactcgt tgcagaccgg catgatgcgt tcgaggaagt atttgaggtt ggcaaacagt
     5401 ttctcatcat ccacatcgcg gtaagcgtca aagagctctt tcacttttgc catgcgctcc
     5461 ggctcccagc caggaagaat tgcgccgttt gcatcacctg cgatagattc aaacattttt
     5521 tccggaacaa gtgcgtctac ggcggactgt ttgtaggcaa gtacggtcga gccgtccgga
     5581 cggacgcgtg cgagttctgt tctggtccag tcgaagaccg gcatgaagtt atagcagaca
     5641 aggtgaatgt cttcctcgcc gaggtgcttt aacgtttcaa tataattatc gatgtactgc
     5701 tcacggtccg ggctgccgat tttgatggcg tcgtggatgt tgacgctctc gatgccggag
     5761 atgtgaagtc cagaggcctc aacctcttct tttaacgcgt gaatcgcttc gcgtgtccac
     5821 acctcgcccg gggtagtgtc gtacagggtt gtgatgactc cggttacgcc cggaatctgg
     5881 cggatctgct ctaaggtaac ggtatcaaat ttggaaccat accagcgaaa tgtcatttcc
     5941 ataagaaaaa ctctccttta ttttgtaatg tactcccgga gtctttctgc acttgccttt
     6001 gcggctgatt ttccgccagc tgcacccgaa tttcatcaac gtacgcaccg cgtacgcctc
     6061 agaaatttgg gcacaactga caaaaaatca tctcacaaaa tcaagcacag aaagattccg
     6121 agagtacatt gtattttgca agtggtgttc tttgcttttt attgtatcgt ttccaaagaa
     6181 aaaagccatg gcaagaattg ttgaacacat tgcaaaagtt gcggcggttg gccgggaaaa
     6241 agcaaaatca gcctacaggt gtgataagta atatctttct ggtatgacta accaatagga
     6301 aaaacctata agcgattcat cgaaataaca attaccaaaa ggcgttttag gggttgcaca
     6361 tagaaaaaat atgcattata atatgccatg taatcgagaa acacattaaa catatcgact
     6421 tagtaggaaa ggagaacagc aaatgaaaaa aacagacaac agacgaatgc aggagaacag
     6481 aatgatggag atgtgttgtt gctgttgctg cagctccaat cagaataaag aaaaataaga
     6541 aacattcgca ggcagaaaaa gcatagcaga aattgcatgt gcgggcaggc cggaacgaat
     6601 gcctgctatt tttttaacct tttttaaaag aaatgcgaat gaacaacata agaatagata
     6661 ataaacagac atagaaaacg gaggacaaaa acatgagcgg atgcggatgc agcttcaccc
     6721 cagttgaaaa taaagaaacc gaagaaatca aatacaccga tgcactggca gaacagtttg
     6781 cagcagaagt cggagtagac ccaagaccaa atgaaaccct tgtagagatc gatgagcgcg
     6841 gcgctttcat ccgtcagcca aatgcattta tccagccatt cggcgataaa gaaggcgacc
     6901 tgaaagcaga agcaaaccgc ttcgggatct actgggcaac cggatgcaac tggtccaacc
     6961 gtccgatcat cgtgcgggag cttttaggac tgcaggatgt gatctcggag acgagagttt
     7021 ctccatccgg cgagacaaac cgttacggac atgcattcgg acagtatccg gatttcaagg
     7081 atccggcgac aggcgcttac ttcttaagtg agttctataa aagagcaaac ccggacttca
     7141 aaggaagagc aacgacaccg accctggtgg atgtcaaaga gaaaaaggct gtgaacaacg
     7201 attatcaccg cctgacgaac tacctcgaag tgcagttccg tccattccag ccaaaagatg
     7261 cgccggacct gtacccgaaa aaattccgga aagagatcga tgagtttaac gactggctct
     7321 tcccgcatgt caacaacggc cattaccgca tggcattctg ccagtcaccg gaagcatacg
     7381 atgaggcata cgaagatttc tatgaatccc tcgataagct ggacaaacgg ctggagacga
     7441 accgtttcct gttcggcgac tacatcaccg acagcgatgt ccgcgcttac gtcactctga
     7501 tccgttggga tgtcagctac ttccacaacg taggaccggt gaagaaaccg attcgtgatt
     7561 acaaaaatat ctggggctac ttaaaagagc tgtatgcgat cccggccttc cgtcacggaa
     7621 gcgatccgca ggtgctggca ctttcgagac ctggaaagaa attaggcgag gttctcttcc
     7681 gcggatacaa cgaacgaatc cttgcaaaag tagatttcga gaagctgatg gcagacgatg
     7741 gatcgagaag aacactcagc aaaacaccgg acgaagtatt ccttcgccat ccggagggag
     7801 agacctacga ggattacgca ggagaaatct caaaaaccat ctggaacagc ccgaaatggg
     7861 aagacagaaa cccgaagaac ggcgtacttt ccgtggatgc atcgattaat ccgctgaaag
     7921 gacttttata agaaaaacag aagaattcgg ggcaccttgg tggaacactc tcaggcagcc
     7981 ggggcgcggc cggaacagaa aaaaacgagg aaatgaggag gattttatta tgaaaaagac
     8041 aaagaaattc tgggcagtat tggcagcggc gggccttttg gcagcaagct ttgcaggatg
     8101 cggaagcaat gcatcagcaa atgcaggaag cagcagcggt tccgcagcat catcttccgc
     8161 agaaggcagc tccgcatcgg attccggtga gaaaaaagtg atcaaagctg taacaagcgg
     8221 cacactggca ccgtactttt acgtaaatga tgacaacgag ctgaccggtg tcgatatcga
     8281 tatcgtaaaa gaagtgttca accgccttcc gcagtatgaa ctgaaaattg agcaggcaga
     8341 tgcactgcag ggtgtgctga gcggtcagta cgacatcgca gtcaacaact atggatatac
     8401 cgatgagaga gcagaatcct actacttctc tttaccatat aaaaccagct tcaacgaata
     8461 cattcagaga accggcgatg agccgctgac ttctcttacc gatctggcag accgcggata
     8521 caaaattgag ctgagtgcag gaagccttac cgcaaatgca ctggaaaaat ggaatacaga
     8581 caacccggat caccagatca atatcgtata ttccaatgca aacttccagg tccgctacca
     8641 gcacatcgta gatggaaccg ctgatgtggc aatcgacgac ggaccgatca tcgacaacct
     8701 gcttccgcag ttcggactgg aaggtcagct ggaagcaaac gagatcgacg aggagaccga
     8761 agatttcctg ttcccgcaga acaataccta cttcctgttt ggaaagaacg aagagggcgc
     8821 agcactccgc gaggatgttg acaaagcctt aaaagagatg aaagaagacg gaactctttc
     8881 caagatcaca accgagtatc tcggcgtgga caccagcccg gacgagaaat actttgatga
     8941 gacaccaaac tagaatatcg ttttcgaaat aaaaacagaa gagattacaa aacagacggg
     9001 aagcggatcg agaacatccg tttcccgatt ctaaaaggag cgaaaaacat ggcagaaatt
     9061 taccgggtag aaaagggaag cgaagagtgg aaatacaaag catatgatta cgtcagaacc
     9121 gatgcgttct gcttgggaca gaacattccg attgaactgg aattcggaca ggacgagagc
     9181 aaagatgatc tgcaggcgat tctgctgaca gaagatcaca agccggtagc cggctgccgc
     9241 atcgcatttc cggcggaaaa gacaggaaaa atcgagcgtg tctgtgttgt cagagaaaaa
     9301 cagaaaagcg gatacggccg cgtactgatc gaagccgctg agaaatggct gaaagaatac
     9361 ggtgtcgatc acatcgtcat cacgagccag gaccgcgccg caggctttta tgagaaatta
     9421 ggctacaaac tgaatccgga cgctgacctg tcggtatacg accaccatgt accgaagaaa
     9481 gacgaagagc cgaaaaagcc gaagaaaaat cttggcttta cctgtgtact ggtagaaaaa
     9541 tacgtgtaag agaggaaaaa aatcatggga aaattatttg acgtttcact ggtattttca
     9601 tccataccag aattgctgac aaagctgccg atcacgctgg aactggccgt ggtttctatg
     9661 ctgatcggac tgggactggg acttgtgatg gcgatcatca aaatgaaaaa aatcagagtg
     9721 ctgacgcaga ttacgaatct tctgatttca ctgctccgcg gcacgccggt catcgtacag
     9781 ctgtacgttg cgttctttgg tattcccatg atgttcaagg caatcaacca gcagttcgga
     9841 acgaatctgg cggttgccaa tatttccggt ttcgtctatg cgatgatcgc tctgggactg
     9901 aaccagtcgg gctttatggc tgagattatc cgttcggctt tgcagtccgt tgacccgggc
     9961 cagatcgaag cagcacatgc actcggcatg acgtatccgc agacactgcg ccgcatcatt
    10021 ctgccggagg cctttgaggt tgcgcttccg acccttggaa actcttttat cagtatcatc
    10081 aaaggaacgt ctcttgcctt tacctgcgcc gttgtcgaga ttaccgcaca gggccagatt
    10141 attggaggaa gaacgtatcg ctactttgaa gtttacgttt cactggccat catttactgg
    10201 ctggtaacgc tggtgatcga gcagattatc cgcgtaattg aaaataaaat ccggattccg
    10261 gaccaggtag agctggcgtc cgcaaacggg gagggtgaga aaaatgataa aaattgatca
    10321 tttatcaaaa aaatataacg acaacgtggt gctcgacgac atcagcctcg agatcaaaca
    10381 gggcgatgtt gtgggaatca tcggtccttc cggaaccgga aaatcaacgc ttctgcgctg
    10441 cgtcaaccgc cttgagacac cggagaaggg aacggtgacg atcggggaca aggtgatcgg
    10501 cctttccgag cggaaaccga aagagcttct gtatttaaga cagaacacag gaatggtgtt
    10561 ccagcggttc aatctgtttg agaagaaaac cgcactggaa aatgtgatgg aaggcctgat
    10621 tgttgtcaag aagatgaaaa aagacgaggc cagagccatt gcattagaag aactgaaact
    10681 ggtcggcatg gagaaatggg caaaccacta tccgaaacat atgtccggcg gacagcagca
    10741 gagggtggcg ctcgcaagag ccctcgccat gaaaccgcag attctgctgc tcgacgagcc
    10801 gacctcagcc cttgacccgg agctggttgg agaggttctg gatgttatta aaaaagttgc
    10861 ggcacaggga tatacaatgc ttctggtttc ccatgagatg aactttatcc gccatgtgtc
    10921 gaaccgtgtg atcttccttg ataaaggaca cgtagtagaa gacggaacac cgaaagaagt
    10981 attcgagcat ccgcagaatc agagaacacg ggagttcttc agcaagatgc atattatggc
    11041 gcagcctgag tatgttattt aaattgagca gtgccggaat gcagtgaagc aggaaatttt
    11101 gtgctcgcac gaaattttct gttttgctgt attctgatag ggcgaaagcc cgaagcccag
    11161 ggaagcgaag cttttctggg ccgcactgcg gacacagcca aagagtagga gaaaatatta
    11221 tgatagatga gaaaagaatt gctgattttt ttgcggagct ggtatccatc gattcgccgt
    11281 ccttggagga gtgcgagatg gcggacactc tgaaagcaaa attcgccgaa atcggagtga
    11341 attttacaga agatcataca caggagcaga ccggttcaaa tgcgggcaat ctgtttgcac
    11401 gtatcccggg cagtattgac ggtgcgccgg ttctctttgc cgcccacatg gacacggtgg
    11461 agcccgcaaa agggaaaaag gctgttttcc atgatgacgg aaccgtaacg agcgacggca
    11521 caactgttct cggcgccgat gatctggccg gtgtgaccgc gatctatgag gcggtgcgcc
    11581 acatcactga ggcaggagcg ccgcacagcc cgatcgaaat cctgatttca accggtgagg
    11641 agctgtactg caaaggtgcg aatgccttcg attacagcca ggtgcaggca aaagaagcgt
    11701 acgttctgga cctgagcgga gcaatcggcg cggcagccta tgcagctccg acccttgtat
    11761 cttttaaagc agaaatccag ggaaaagccg cccatgcagg ttttcacccg gaggacggca
    11821 tcaacagcat ccaggcggct gcacgtgcag tcgcacagct tccgcagggc cacgtggcag
    11881 agagcaccac agccaacatc ggaaaaatcg gcggcggttc cggcaccaat atcgtttcgg
    11941 agagctgcct tgtcgaaggc gaaatccgca gcctgaacca cgagacggcg atggaacttc
    12001 tggcgacata tcagaaaacc ttccagcagg aggcagacgc catcggtgcc aaacttgtct
    12061 gggaagaaaa gctgaacatt catgcctatg agacaccgct gacgggcgtg gccgcaaaac
    12121 actatgagag cgccgttgcc aaagagggtc tgaccgccgc attcgaaaaa accttcggcg
    12181 gcagcgacaa caacgtcttc gcccagcacg gcatcgaggg cctcgtcatc gcaacatc
//
LOCUS       DAOMUF010000050        12201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1199_length_12201_cov_3.201712, whole genome shotgun sequence.
ACCESSION   DAOMUF010000050 DAOMUF010000000
VERSION     DAOMUF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_12201_cov_3.201712"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /gene="acsE"
                     /locus_tag="PLN77_04460"
     CDS             <1..666
                     /gene="acsE"
                     /locus_tag="PLN77_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase/acetyl-CoA
                     synthase methytransferase subunit"
                     /protein_id="HRM43566.1"
                     /translation="GAVYLDVNIGPAENDGEDIMKWAVELLQSNFDNVPLALDTSNKK
                     AIEAGISVYNRSKGKPIVNSADAAGRIEYVDLAAANDAIVIALCNGEGIAKDNDERMM
                     YCQTLLERGMEHGMEPTDLWFDPLFLVVKGMQDKQMEVLECIKMFSDMGLNSTGGLSN
                     NSNGMPKNIRPIMDSALVAMAMMQGLTSAIVNPNDLRLMETIKSCDVFKGNTLYADSY
                     LEL"
     gene            804..2729
                     /gene="acsV"
                     /locus_tag="PLN77_04465"
     CDS             804..2729
                     /gene="acsV"
                     /locus_tag="PLN77_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid activation/regeneration protein AcsV"
                     /protein_id="HRM43567.1"
                     /translation="MAKVTFRFEEGEPVVTYATEGERLLEVAQKSNVPIDAPCSGNAS
                     CGKCRVRLVSGELDSKITRHISEEEYQNGWRLACVSTIRGDVEVEVPDIASAYRSRMK
                     VADLSSPSEIAIFEDTKKKITDAGLELKNSMQVITISMEEPTLDDTMPDNERVTWAVQ
                     AATGLDRVRIPYSVLKKMPDVLRESHFQAQCVVRVTANDVFLYDMLPMEAKAVVGGLV
                     VDIGTTTVSALIVDMLSGEILAKASSGNGQIRYGADVINRIIESQKPGGHERLQNAII
                     KETLNPMISNMCRAAKISSQQIYRAAIAGNTTMEHLMMGINADPLRMEPYIPAFFKTN
                     SLFASDVNLAIHPDAHIILAPNIGSYVGGDITAGALVSMIWNRPEMSLFIDLGTNGEL
                     AFGNSDFMVSCACSAGPAFEGGDISCGMRATDGAIEKCTIDPETMEPSYHVIGDEGTK
                     PIGLCGSGIIDVIAALFRAKMVNPKGKFIREGKRIRHDKYGMGSYVIAFEEEAGSVRD
                     VEITEVDIDNFIRAKGAIFSAIRTMLNYLDMDVSMIEEVYVAGGIGSGINMKNAVTIG
                     MFPDIPLEKFHYIGNSSLTGAYSMLLSTAAEKKTYEVARNMTYLELSTVPTYMDEFVA
                     ACFLPHTDTTMFPSVEA"
     gene            2824..3495
                     /locus_tag="PLN77_04470"
     CDS             2824..3495
                     /locus_tag="PLN77_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HRM43568.1"
                     /translation="MAVDLNYEKDSKERIPYEHYLEVYQNADPKEISSRCDVPYDAEK
                     QEFTVSLMGVSYRISWPEYNVFHIGDDGSVSPIIGWYPLEKKPNAKILVLRYLTEGGA
                     APSTGKFLTYREIPWGEVYFKQFQGRCLFRLAFGFGGKLDAFREIMERVGAQAISSGD
                     VGYELEFMKGLFVRLILWAGDDEFPPSAQILFSDNFPAAFTQGEDMAVVGDVTIDMLK
                     ALAAK"
     gene            3557..4189
                     /locus_tag="PLN77_04475"
     CDS             3557..4189
                     /locus_tag="PLN77_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HRM43569.1"
                     /translation="MGFSTVPCNEFVEVLGSKAPVPGGGGASALVGAVGTALGNMVGA
                     LTVGKKKYADVEEEMKELMAKATTLQDELLHLIERDAEVFEPLSKAYGMPRETEEEKA
                     EKARVMEIVLKDACSVPMEIMEKCCEAIDLIVEFAAKGSALAISDAGVGAAFCKAALE
                     GASLNVYINTKSMKNREYAEELNRKCDEMLAVYTVKADEIFRNVLGRLKA"
     gene            4277..5128
                     /locus_tag="PLN77_04480"
     CDS             4277..5128
                     /locus_tag="PLN77_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRM43570.1"
                     /translation="MAKQLLGKEVTAALNEKIKANVAELEAKGVKPTLGIIRVGERED
                     DLSYERGATKRCETLGVAYEKFLLPADVTQEELMATIDKVNKDDSIHGVLIFRPLPKH
                     LDEAAVIKALSPEKDVDGITDGSMVGVFAGTKQGFPPCTPQACMEILDHYGIDCTGKK
                     AVVVGRSLVVGKPAAMMLLKKNATVTICHTRTKDMPSVVKEADIVIVAAGRAGVVDDT
                     YLRAGQTVIDVGINVNEEGKLCGDVAYAKAEPIVEAITPVPGGVGSVTTSVLVGHVVE
                     AAMRKNS"
     gene            5300..6769
                     /gene="spoIVA"
                     /locus_tag="PLN77_04485"
     CDS             5300..6769
                     /gene="spoIVA"
                     /locus_tag="PLN77_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HRM43571.1"
                     /translation="MDAYSVYRDIQKRTKGQFLIGVVGPVRTGKSTLIRRFLEVLALP
                     YLSEKEQAEVRDALPLSGEGKTITTVEPKFVPKDAVELQLGEGDPIRLRLIDCVGFLV
                     PDAAGNLENEKERMVKTPWSEQAIPFREAAEIGTKKVIAEHSTIGLMVTCDGSFGELP
                     RENFLQAEERTVAELKKQGKPFLILVNAKKPYREETQRQVKELETKFGVAAMAVNCDQ
                     LRKEDILHMLEKVLYEFPLQQMEFYIPKWVELLADDHPLKAELLQKIREMCREKTRLK
                     DAVGGKTAIASQYVSQTLLQSMDLASGTVKVRIEVKEQYYYEMLSVLAGVEIKGEYDL
                     VQTIRELSAQREEYRKVEDAIHAVRGCGYGVVIPGREEIQLEEPAVIHQGNKYGVKIK
                     SVSPSIHMIKANIETEIAPIVGSEQQAEDLIAYIKESAKSPEGIWNTNIFGKSVEQLV
                     DDGIRSKIAQITEDGQAKLQDSMQKIVNDSRGGMVCIII"
     gene            6874..7068
                     /gene="ssrS"
                     /locus_tag="PLN77_04490"
     ncRNA           6874..7068
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLN77_04490"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7139..8182
                     /locus_tag="PLN77_04495"
     CDS             7139..8182
                     /locus_tag="PLN77_04495"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRM43572.1"
                     /translation="MKKQGFGTTKDGKEALLYTLSNKNGMEISVTDYGAHLVSVLVPD
                     KDGKKRDVVLGFDSVTGYETDGSHFGATIGRNGNRIAGAAFELHGKTYQLAKNENNNN
                     LHSGPDGYDYRLWNVEEADDSAVTFVLMSPDGDQGFPGNFEVSVTYTLTDENAVEIHY
                     EGSCDQDTVVNMTNHSYFNLAGHDAGSIENQTLVLKAEQFSPVIDSASIPTGEHAPVQ
                     GTPMDFTSEKAIGAEIEADFEQLKLTGGYDHNFILDKAVEGSIELMAVASCKESGITM
                     EAFTDLPCVQFYAGNFIAPVTGKNGVFYDKRNGFCLESQYVPNAINQEGEEKPILEAG
                     DTYDTTTVYKFIF"
     regulatory      complement(8757..8958)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9081..10499
                     /locus_tag="PLN77_04500"
     CDS             9081..10499
                     /locus_tag="PLN77_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRM43573.1"
                     /translation="MELILLGVGILLLLLLFVVKRRLDAPEETATAESRTETMEGKKQ
                     EETIQNMEQENAAFLNSKKEEQKETTAAGPVQIRVLLTDCGKLGYAQKQVSLRGNASL
                     SMNDGAYIASPQETICVTEQECGEVVHVVPADETAGTAVSLTGKPEDETVYPGSFVIY
                     KSEEGLLVVNELDLEMYLRYVVPSEMPSSYEKEALKAQAVCARTYACARIREKTWENY
                     HADVDDSVESQVYHNMEAQPETDAAVAETEGKIITCGGEPIQAYFFSTSCGKTSTDEV
                     WNTAETAEYLKSVTVGGEKQEPETEEAFASFITKRDSTSLEVEDGWYRWQVTIPADVL
                     SERAADQKIGEITGIEVRRRSEGGAAETVLIEGEKGSMEIEGEYQIRKFLSAKGWPIQ
                     KNDGTVSCEMELLPSACFIVVPEKEGETAVSFTFYGGGYGHGVGMSQNGAKHLAEQGK
                     SYEEILRYFYQNIEIGDCRSLF"
     gene            10578..11555
                     /locus_tag="PLN77_04505"
     CDS             10578..11555
                     /locus_tag="PLN77_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM43574.1"
                     /translation="MATIKDIAARAGVTATTVSRVINNRGYISEETRKKVTQAMQEMN
                     YQPNELARSLAKAHSNTIGVIVPHISHPFFSKMISALESAAAKRGYKILLCNSKDQPE
                     KETEYLDMFISNRVTGVVMASRDVQIEKFHDLKIPIVNFEREENTEAITIQCDNEQGG
                     ALAASHLADCGCCHLLHFGGIVGRDMPADRRADGFLRVCRERGIEGEVLLSDRLSYGS
                     MHYESYIEKGLLEHPETDGIFASSDLIAAQVLQRLYAMGKRVPEDIKVVGFDDTVIAG
                     ITTPSITTIHQPIEEMSELSIAYIDRRRKGEMVPNVTTMPVSLVVRDSA"
     gene            11808..>12201
                     /locus_tag="PLN77_04510"
     CDS             11808..>12201
                     /locus_tag="PLN77_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM43575.1"
                     /translation="MTVEYQRRLEKHYDELKWLYCELYPNGQGRFEELCASMEQWYKE
                     RNKKWKALDRKREKQKNWYKSQKMLGMMLYIDAFADNISGLEEKLDYLKACNVNYLHL
                     MPFLATPKGKSDGGYAVADYRTVQPSLGT"
BASE COUNT         3525 a         2641 c         3420 g         2615 t
ORIGIN      
        1 ggtgccgttt accttgatgt aaacatcgga ccggctgaaa atgatggaga ggacatcatg
       61 aaatgggctg ttgagctgct gcagagcaat tttgacaatg ttccgctggc tctggataca
      121 tccaacaaaa aagcaattga ggctggtatc tctgtataca accgttccaa aggaaaaccg
      181 atcgtaaact ctgcagatgc tgcaggaaga atcgagtacg ttgaccttgc agctgcaaac
      241 gatgcaatcg ttattgcact ttgcaacggt gaaggaattg caaaagacaa cgacgagcgt
      301 atgatgtact gccagaccct tctggaaaga ggtatggagc atggcatgga gccgacagat
      361 ctgtggtttg acccgctgtt cctggttgta aaaggaatgc aggacaaaca gatggaagtt
      421 cttgagtgca tcaagatgtt cagcgatatg ggactgaatt ctaccggcgg actttccaac
      481 aactccaacg gtatgccgaa aaacatccgt ccgatcatgg attccgcact tgtagctatg
      541 gctatgatgc agggtctgac cagcgccatc gttaacccga atgacctgag actgatggag
      601 accattaaat cctgtgacgt attcaaaggc aacactctgt atgcagattc ttatctggag
      661 ctttaatctt taaatctcgc aggttttccc ctgatggtgc cgcagcgttt gcgaaaacct
      721 gaggcaccat caggggtttt tctatctaaa agcggaaaaa taaaaaaagc ggagaatata
      781 tacgactggg ggatttacag attatggcaa aagtaacctt tcgatttgaa gaaggagaac
      841 cggtagtcac ctacgcaacc gagggtgagc gccttcttga agtagcgcag aagagcaatg
      901 tgccgatcga tgcaccgtgt tccggaaacg catcgtgcgg taaatgccgc gttcgtcttg
      961 tgagcggtga gctggattcc aaaataaccc gccatatttc tgaggaagaa tatcagaatg
     1021 gatggagact ggcgtgcgtc agcaccatca ggggcgatgt tgaagtggaa gtgccggaca
     1081 ttgcatccgc ttaccggagc cgcatgaaag tggcagacct tagttcaccg agtgaaattg
     1141 caatttttga agatacgaaa aagaaaatta cagacgccgg cctggaattg aaaaacagca
     1201 tgcaggtgat tacaatctca atggaagagc ctactctgga tgatacgatg ccggataacg
     1261 agagagtaac ctgggctgta caggcagcga cggggcttga ccgggtacgg attccgtact
     1321 ctgtactgaa aaaaatgccg gatgtccttc gggaatccca ttttcaggca cagtgtgtcg
     1381 tccgggttac ggcaaatgac gtctttttat acgatatgct tccaatggaa gcaaaagcag
     1441 tggtcggtgg tctcgttgtc gacatcggaa caacgacagt ttctgcactg atcgtggata
     1501 tgctgagcgg agaaatcctg gcaaaagcct cttccggaaa cggacagatc cggtatggtg
     1561 cggatgttat caaccgtatc attgaatccc agaaaccggg aggccatgaa agactgcaga
     1621 atgcaatcat caaagaaacc ttaaacccga tgatttccaa catgtgccgc gcagcgaaaa
     1681 tcagcagtca gcagatttac cgtgcggcga ttgccggaaa cactaccatg gagcatctga
     1741 tgatgggcat caatgcagat ccgcttcgga tggagccgta tattccggcc ttctttaaga
     1801 caaactcact gtttgcatcg gatgtcaatc ttgcgattca tccggacgcc catatcattc
     1861 tggcaccgaa catcggaagc tatgtgggcg gagatattac tgccggcgcg ttggtcagca
     1921 tgatctggaa ccggccggag atgagtcttt ttattgacct tggtaccaat ggcgagcttg
     1981 catttggaaa ctctgacttt atggtaagct gtgcatgttc ggcaggaccg gcctttgagg
     2041 gcggcgatat cagctgcggc atgcgtgcga ccgacggtgc gattgaaaaa tgtacgatcg
     2101 atccggagac gatggaacct tcgtaccatg ttatcggaga tgagggtacg aaaccgatcg
     2161 gcctttgcgg ttccggtatc atcgatgtga ttgcggcgct gttccgcgcg aagatggtca
     2221 atccgaaggg taaatttatc cgtgagggaa aacggatccg ccacgacaaa tatggcatgg
     2281 gaagctatgt cattgcgttc gaggaggagg ctggaagtgt ccgcgatgtt gagattaccg
     2341 aggtcgatat cgacaacttt atccgcgcca agggcgcgat tttctcagcg atccgtacga
     2401 tgctgaacta tctggatatg gatgtttcca tgatcgagga ggtttacgta gcgggcggta
     2461 tcggaagcgg catcaacatg aaaaatgccg ttaccatcgg tatgttcccg gatattccgc
     2521 tggaaaaatt ccattacatt ggtaactctt ccctgaccgg tgcgtactca atgcttttat
     2581 cgacagcagc agagaagaaa acgtatgagg ttgcccggaa catgacatat ctggaactct
     2641 ctacggtacc aacctatatg gatgaatttg tagcggcgtg cttcctgccg cacacagaca
     2701 cgacgatgtt cccgtctgtg gaagcataaa acggaagcag aactttctgt tacgattcaa
     2761 ggtgaaacac atgtggtttt gctttgaatt gtggcgaacc tttttgagag gaattggatt
     2821 tacatggcag tagacttaaa ttacgaaaaa gacagcaaag agcgtattcc gtatgagcat
     2881 tatctggaag tgtatcagaa tgcagatcca aaagagattt ccagccggtg cgacgttccg
     2941 tatgatgcgg aaaaacagga gtttaccgtt tccctgatgg gtgtttccta ccggatttcg
     3001 tggccggagt acaacgtatt tcatattgga gatgatggaa gcgtttcgcc gataattggc
     3061 tggtatccac tcgagaaaaa accgaatgca aaaattttgg tattgcgtta tctgacagag
     3121 ggcggagctg ctccgtctac cggaaaattt ttgacgtacc gggagattcc gtggggagaa
     3181 gtttatttta aacagttcca gggaagatgc ctgttccgtc ttgcgtttgg ctttggcgga
     3241 aagctggatg cattccgtga gatcatggaa cgtgtcggcg ctcaggcgat ttcatccggt
     3301 gatgtcggat atgagctgga gtttatgaaa gggctttttg tacgactgat tttatgggca
     3361 ggagatgatg agtttccgcc gtccgcgcag atcctctttt cggacaattt cccggctgca
     3421 tttacgcagg gagaggatat ggcggttgtg ggagatgtca caattgatat gctgaaggct
     3481 ctggctgcaa aatagttatg gtttgaggcc atcaggatgg cttctggatc gaaaaaaata
     3541 agcggaggaa aataacatgg gattttcaac agtaccttgc aatgagtttg tagaagtgct
     3601 cggttccaaa gcaccggttc cgggcggtgg cggagcttct gcactggttg gcgctgtcgg
     3661 cacagcgctt ggcaacatgg ttggtgcgct gacagtaggt aaaaagaaat atgcggatgt
     3721 cgaagaggaa atgaaagagc tgatggcgaa agcaaccacg cttcaggacg aactgcttca
     3781 cctgatcgag cgcgatgcag aagtgttcga accgctctcc aaagcatacg gcatgccgcg
     3841 cgagacagag gaggagaagg cagaaaaagc aagagtcatg gagattgtgc tgaaggatgc
     3901 ctgctctgtt ccaatggaaa ttatggaaaa atgctgcgaa gcaatcgacc tgatcgtaga
     3961 atttgcagca aaaggttctg cacttgcgat cagtgacgca ggtgttggcg cagcattctg
     4021 caaagcagcc ttagagggtg cttccctgaa tgtatatatc aacacgaaat ctatgaaaaa
     4081 ccgggaatat gcagaagaac tgaatcgaaa atgcgatgaa atgcttgcgg tctataccgt
     4141 aaaagcagac gagattttcc ggaacgtact gggaagactg aaagcctgaa aacaaacggc
     4201 cagagaagct tcccggcagc aagcgggttg caaaaggagt tctctttgac cgaaaagaaa
     4261 agcggaggat aaaaaaatgg caaaacagtt attaggaaaa gaagtaacag cagcattaaa
     4321 tgagaagatc aaagcaaatg tagcagagct ggaagcaaaa ggagtaaaac cgactctcgg
     4381 aatcatccgt gtcggagaga gagaggatga cctttcctac gaaagaggag caaccaaacg
     4441 ctgtgagacc ttaggcgttg catacgagaa attccttctt ccggcagacg ttacccagga
     4501 agagctgatg gctaccatcg acaaagtaaa caaagatgat tctatccatg gcgttctgat
     4561 tttccgtccg cttccgaaac atctcgatga ggcagcagtc atcaaagcac tctccccgga
     4621 gaaggacgta gacggtatca cagacggttc catggtaggc gtattcgccg gaacaaaaca
     4681 gggcttcccg ccatgtactc cgcaggcctg catggagatc ctcgaccact acggtatcga
     4741 ctgcaccggc aaaaaagctg ttgttgtagg ccgcagcctc gttgtaggaa aaccggccgc
     4801 catgatgctg ttaaagaaaa acgcaaccgt aaccatctgc cataccagaa cgaaagacat
     4861 gccgtctgta gtcaaagagg cagatatcgt gatcgtagca gcaggccgtg caggtgttgt
     4921 agatgatact tacctgcgtg caggccagac cgttattgat gtcggaatca acgtaaacga
     4981 agaaggaaaa ctctgcggag acgttgcata cgcaaaagct gagccgatcg ttgaggcaat
     5041 cacaccggtt ccaggcggcg taggatccgt taccacatct gttctggtcg gccatgtggt
     5101 agaggctgcc atgagaaaaa acagctgatc ataggctgaa gataagaatg tgacagaaaa
     5161 aaggcactgt gcgtccgaaa aaagggcggc ggtgcctttt ttgcgtgcgc ggcagtgcag
     5221 ttttggtgat aggagagggg gccggctcat agaatataac agtacatagc aacaaagatc
     5281 aaaaggggga caacagcgca tggatgcata ttcagtatac cgcgatattc agaaacgaac
     5341 gaagggccag ttcctgatcg gagtggtggg gcctgtacgg acgggaaagt ccaccctgat
     5401 ccgccgtttc ctggaggtgc tggcactgcc gtatctttcg gaaaaggaac aggcggaggt
     5461 acgggatgcc ctgcctttaa gcggggaggg aaagacgatc acaacggtgg agccgaaatt
     5521 tgtgccgaaa gacgccgtgg agctgcagct tggggagggc gatccgatcc ggctgcgact
     5581 gatcgactgc gtcggctttc tggtgccgga cgcggcggga aatctggaaa atgagaagga
     5641 gcggatggtg aaaacaccgt ggtccgagca ggcgattccg ttccgggagg cggcggagat
     5701 cggtacgaag aaggtgatcg cagaacattc cacgatcggt cttatggtga cgtgcgacgg
     5761 atcgttcggc gagctgccgc gggagaattt tctgcaggcg gaggaacgga ccgttgcgga
     5821 gctcaaaaag cagggaaagc ccttcctgat tcttgtgaat gcgaaaaaac cgtaccggga
     5881 ggagacacag cggcaggtaa aggagcttga aacaaaattc ggcgtggcag cgatggcggt
     5941 gaactgcgac cagctccgca aagaagatat tctgcatatg ctcgaaaagg tgctgtatga
     6001 gtttccgctt cagcagatgg aattttatat tcccaaatgg gtggagcttc ttgccgatga
     6061 ccatccgctg aaggcagagc tgctgcagaa gatccgtgag atgtgcaggg aaaagacgcg
     6121 cctgaaggat gcagtgggcg gaaaaacagc gattgccagt cagtatgtca gccagacact
     6181 gctgcagtcg atggatcttg cgagcggtac ggtgaaggtg cggattgagg tgaaagagca
     6241 gtattattat gaaatgctga gtgtacttgc cggcgtggag atcaaaggcg agtatgatct
     6301 ggtgcagacg atccgcgagc tgtcggcaca gcgggaagag taccgcaagg tagaggacgc
     6361 gattcatgcg gtgcgcggat gcggctacgg cgttgtgatt ccggggaggg aggagattca
     6421 gctggaggag ccagccgtga tccatcaggg aaacaaatac ggcgtgaaaa ttaaatcggt
     6481 gagcccgtcg attcatatga tcaaggccaa catcgagacg gagattgcgc cgatcgtcgg
     6541 aagtgagcag caggcggagg atctgatcgc ctatatcaaa gagagcgcaa agagcccgga
     6601 gggaatctgg aataccaaca ttttcggcaa atccgtcgag cagctcgttg acgacggcat
     6661 ccgcagcaaa atcgcccaga tcaccgagga cggccaggca aaactccagg attccatgca
     6721 gaaaattgtc aacgacagcc ggggcggaat ggtgtgtatt atcatttaat acaaaagctg
     6781 ttcgaatatt tttgagtaat tgtgcaataa tacttgaaaa gcgaaccaaa atagcgtata
     6841 atccgaattg tgaaaagaaa agaagaaaaa tgtaacttcc tggaatgttc gatgatctca
     6901 ttattttgaa cctacattta ctttaaacgg gaggcttatt attgtcatgt ggatgtcagg
     6961 ccaccctgga aacagacgtc agtggaaggc agaaatgatg actgcggcta cccacctagc
     7021 ttgcgttgct aggagtcaaa gtatgggaaa cggcattccg gggttacaaa agagaaaaaa
     7081 gagaatcatg aaaatggttc tcttttatac gttacataca accaaaagga gaaggaacat
     7141 gaaaaaacag ggttttggaa caacaaaaga cggaaaagaa gcattgctct acacgctctc
     7201 caataaaaac ggaatggaaa tcagtgtaac cgattacggc gcacatctgg tatcggttct
     7261 cgttccggat aaggatggaa agaagagaga cgttgtcctt ggatttgact ccgtaaccgg
     7321 ctatgagacc gacggaagcc attttggagc caccatcgga agaaacggaa accgtatcgc
     7381 gggagcagca tttgaactgc acggaaaaac ataccagctg gcaaaaaatg agaacaacaa
     7441 caacctgcac agcggcccgg acggatacga ttaccgcctc tggaacgtag aagaggcgga
     7501 tgacagtgcc gttaccttcg ttctgatgag cccggacgga gaccagggat tcccgggaaa
     7561 ctttgaagtg tccgttacgt ataccctgac cgatgagaac gccgtagaaa tccactatga
     7621 gggaagctgc gatcaggata ccgttgtcaa catgacaaac cacagctact tcaacctggc
     7681 aggccacgat gccggatcta ttgagaacca gacacttgtc ttaaaagcgg agcagttcag
     7741 cccggtgatc gattctgcat ccatcccgac cggcgagcat gcaccggtac agggaacccc
     7801 gatggatttc accagcgaga aagcaatcgg agcagagatc gaagcagatt tcgaacagct
     7861 caaactgacc ggcggctacg accacaactt tatcctggac aaggctgtcg agggaagcat
     7921 tgagctgatg gcagtcgcat cctgcaaaga gagcggcatc accatggaag ccttcacgga
     7981 tctgccatgc gttcagttct acgccggaaa cttcattgct ccggttaccg gaaagaatgg
     8041 ggtattctat gacaaacgca acggcttctg cctggaatct cagtatgtgc cgaatgccat
     8101 caaccaggag ggtgaggaga aaccgatcct cgaggcaggc gatacttacg acaccacaac
     8161 cgtatacaaa ttcattttct aagatcctat tttatagcaa tccccgaaaa taatgcaaaa
     8221 aattcagcac cgcagagcgt gaattactgc gtcacagatt ttcgtcgtac cccggtacgc
     8281 cttcaaatct gttccttgca attcgcgctc tgcagcactg ccttttttgc attattttcg
     8341 ggtcttgcta tagaaacagc ataaaaaagc tcccttcctg cagcgtcctg tgaaaacagg
     8401 agcgcaggcg gggagccttt ttatgggaaa atatgcagaa aacgaattct gaaaaaacag
     8461 aaaaagaaaa gaccctgttt tcgaatccaa cagaatctta ccagttatgt catattcttt
     8521 tcagaggtgg gtaaccgctt cgccatctga atcaacctgc aagcggaata gcagacacct
     8581 ttaagcggag gatggcgcct gacaaagaac aggcgaaacc agaatctgaa acggtttacc
     8641 cggccgttta atgcaatgca taaatctcgc tcgaaggttt ctaaaaatct aatcgaaaat
     8701 tttcagacat tccatatccc ataatagtct ttgaatggaa gctttcatcg gaagcatcaa
     8761 ccctatgcat acagagcagg tttcctgact tacagatcat cgttccacat tccttctcat
     8821 atgttgcgga catacaatgg atttgttatg cttcactccc tgattacagt gaccggatcg
     8881 cccaggaatc tcacctgaat tccctctctc accaaaagac ggatcttctt ttgatgcact
     8941 ctggatgttg atatgaaatt tatacacaac cgtactatat accatagggc ggtatttgtc
     9001 aaggaaaaat cgcaaaaaga aaaggaatca gacaaaaagc cgtgtgcata gaatgagata
     9061 tgaagagaaa aaatgataaa ttggaactga tactgttagg agtggggatt ctcctgctgc
     9121 tgttattgtt tgttgtgaag cgccgtctgg atgcgccgga agagacagcg acagcagaaa
     9181 gcaggacgga gacgatggaa ggcaaaaagc aggaggagac gatacagaac atggaacagg
     9241 aaaacgcggc ttttctgaac agtaaaaagg aagaacaaaa agaaacaacg gcagccggac
     9301 cggtgcagat ccgtgtcctt ctgacggact gcggaaagct gggatacgca cagaaacagg
     9361 tgtccctgcg tggaaacgca agtctttcca tgaacgacgg cgcatacata gcttccccgc
     9421 aggagacgat ctgtgtgacg gaacaggaat gcggggaagt tgtgcatgtg gtgccggccg
     9481 atgagacggc gggaacggcg gtttccctga cgggaaagcc ggaggacgag acggtttatc
     9541 cgggaagttt tgtcatctat aaaagcgaag agggacttct cgttgtaaat gaacttgatc
     9601 tggaaatgta tcttcggtat gtcgtgccga gtgagatgcc gtcctcctat gaaaaggagg
     9661 cattaaaagc acaggcggtc tgtgcgagaa cgtatgcgtg tgccaggatt cgtgaaaaaa
     9721 cgtgggagaa ctaccacgcg gatgtagacg acagcgtgga aagccaggtc taccacaata
     9781 tggaagcgca gccggagaca gacgctgcgg tggcggagac agaaggaaaa atcatcacct
     9841 gcggcgggga accaattcag gcgtattttt tctcaacctc ctgcgggaag acgagtacgg
     9901 atgaagtgtg gaatacggcg gagacggcag aatatctgaa gagcgtgacg gtaggcgggg
     9961 agaagcagga accggagacg gaggaggcgt ttgcatcgtt tatcaccaaa agggacagca
    10021 caagtctgga agtggaggac ggctggtacc gctggcaggt gacgattccg gcggatgtgc
    10081 tgtcagaacg ggcagcggat cagaaaatcg gggagatcac ggggattgag gtgcggcggc
    10141 ggtcagaggg aggtgcggca gaaaccgtcc ttatcgaggg ggaaaaagga tccatggaaa
    10201 tcgagggaga gtatcagatc cgaaagttcc tctcggcgaa aggctggccg attcagaaaa
    10261 atgacggaac tgtaagctgt gagatggagc tgcttccgag tgcctgcttt atcgtggttc
    10321 cggaaaaaga aggggaaacg gctgtctcct ttacatttta cggcggcggc tacggccacg
    10381 gcgtcggcat gtcacagaac ggtgcgaaac atctggcaga gcagggaaaa tcgtatgagg
    10441 agattctccg ctatttttac cagaacatcg aaatcggcga ctgccgttcc cttttttaaa
    10501 aaaatcaccg aaaacagatt ttttgtgcta taatgggcga aagtgaaaaa cagttagaaa
    10561 atgacgagga gagaaacatg gcgacaatca aggacatagc ggcacgcgcc ggcgttacgg
    10621 cgacgacggt ttcccgcgtg atcaacaacc gcggatacat cagtgaagag acaagaaaaa
    10681 aagtaacgca ggcgatgcag gagatgaatt accagccgaa tgagctggcg cgttccctgg
    10741 caaaagcgca ttccaatacg atcggggtta tcgttccgca catctcgcac ccgtttttca
    10801 gtaaaatgat cagtgcactc gagagcgcgg cggcaaagcg gggctacaag attctgctct
    10861 gcaactcgaa agatcagccg gagaaagaaa ccgaatatct cgatatgttc atctccaacc
    10921 gtgtaaccgg cgttgtgatg gcaagccgcg atgtgcagat tgaaaagttt catgatctga
    10981 aaattccgat cgtcaatttc gagcgagagg aaaatacgga ggccattaca atccagtgtg
    11041 acaacgagca gggaggagcg cttgcagcat cgcatcttgc ggactgcggc tgctgccatc
    11101 tgcttcattt cggcggaatc gtcggaagag atatgccggc ggaccggcgt gcggatggat
    11161 tcctgcgcgt ctgccgggag cgggggattg aaggggaagt tcttttgagt gaccgcttaa
    11221 gctacggctc catgcactat gaatcctata ttgaaaaggg ccttctggag cacccggaga
    11281 cggacggcat ttttgcaagc agtgacctga ttgccgcaca ggtactccag cgcctttacg
    11341 ccatgggaaa acgggtgccg gaggatatta aagttgtcgg gtttgatgac actgtgatcg
    11401 ccgggattac cacgccttcc atcacgacga tccatcagcc gattgaggaa atgtcggagc
    11461 tttccattgc gtatatcgac cggagaagaa agggagagat ggtgccgaat gtgacgacca
    11521 tgccggtgag ccttgtggtg cgcgattcgg cgtgaaacaa aaacgaaaag aaaactctgc
    11581 ggaaaaaaga aaccgcggag tttttttctg ccctgcggta aacgatagac agaaaaatag
    11641 caaaaaagac aaagaagaag ccggaaattt gttgaaaaat atggtatgta aacggttgac
    11701 atataattca cacaacggta tactgcaaga gaagaaacgt aacacagaat taccgttacc
    11761 ggaatgatgt gacaaaataa agaataagat aaaggagaat aaacaacatg acagtggaat
    11821 atcagagaag actcgagaaa cactatgatg agctgaaatg gctctactgc gagctgtatc
    11881 caaacggaca gggaagattt gaagagctct gtgcgagcat ggagcagtgg tacaaagagc
    11941 ggaataaaaa atggaaagca ctcgaccgta agagagaaaa acagaagaac tggtataaga
    12001 gccagaaaat gctcggcatg atgctgtaca tcgatgcatt cgcagacaac atcagcggcc
    12061 ttgaggagaa gctggattac ttaaaagcgt gcaacgtgaa ttatctgcac ctgatgccgt
    12121 tccttgccac tccgaaagga aagagtgacg gcggctatgc ggttgccgat taccgcacgg
    12181 tacagccgtc ccttggaacg a
//
LOCUS       DAOMUF010000051        12011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1238_length_12011_cov_3.147039, whole genome shotgun sequence.
ACCESSION   DAOMUF010000051 DAOMUF010000000
VERSION     DAOMUF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1238_length_12011_cov_3.147039"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PLN77_04515"
     CDS             <1..1300
                     /locus_tag="PLN77_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43576.1"
                     /translation="YSLPTYQTRWNANAFLLTTVIPAILMFVINAVLISGRLRLSPLR
                     FLRHDFAKKGKSHAVRLPNFRFFSRFRLRILLQNKSSYFTLFLGIIFANILLLFGMMM
                     KPLLSHYQTEAVENMLADYQYILNVPDPIDEDDEYTLMGIVQKLLTPSLKTNTEGVET
                     FALTSLKNIPKNREGESISVYGIQKDSKYVSAELFENGVTISDGYAEKYGMKVGDTLE
                     LKEPYEDKTYSFEVKSIYAYPGALAVFLPMDVFCEEFDHPEDYFNGYFSNEPITDLEE
                     SYIATKITEDDMTKISRQLNVSMGEMFQLINVFSIVLFMLLIYLLTKLIIEKNTTSIS
                     MVKILGYENREILSLYLTATTWVVIVSIGASLIIASALIRLIYVIMLSEYSGWLTYYI
                     DPAIYGKMYLMGLAAYAVIAVLQFRHIQKIPMDEALKNVE"
     gene            1500..2828
                     /locus_tag="PLN77_04520"
     CDS             1500..2828
                     /locus_tag="PLN77_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM43577.1"
                     /translation="MNENFMKEKPVFPLLVSMALPMVISMLVNSLYNIVDSFFVAQIS
                     EDAMTALSLVYPVQNFVNAVAIGFGIGLNAVISYYLGAGDKKAAGCAATQGLALTMLH
                     AVILTIGSLLVIERFLRMFSSSETVIDLGVRYSRIIFLFTIAIMANLYFEKVFQAVGR
                     MKVTMTGMMIGCIINIILDPLIIFGIGPFPRLGIEGAALATGIGQVTPVVIYWIIYKI
                     RPISVKIRPEYLKEGKNLVGRLYAIGVPAILNLALPSLLISALNGILAVYSQSYVVVL
                     GIYYKLQTFIYLTANGIVQGMRPIIGFNYGAGEHKRVRKIYEITLIMCAVIMLIGTGI
                     CLAIPEQLIGLFSSNPETIRLGGQALTRISAGFLISAVSITSSGALEGLGKGGSSLII
                     SLCRNVIVILPAAYLLSHLGGGVFVWHAFWITEVISAAVSLVVYRRAVRR"
     gene            2978..3676
                     /locus_tag="PLN77_04525"
     CDS             2978..3676
                     /locus_tag="PLN77_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM43578.1"
                     /translation="MEETNILVVDDEKEIADLLEIYLISDGFQVLKAYSAKEGLRILE
                     EKKIDLVLLDVMMPDMDGIAMCRRIRETNNIPIIMVSAKTQDIDKIVGLSTGADDYVA
                     KPFNPLELMARVKSQLRRYRELNPSKPHERQEVISLKHLEINKVTHQVVCYGESIKLT
                     PIEFDILYLLASNPGRVFGTDEIFERVWHEKFYEANNTVMVHIRRLRGKMKDDTREDK
                     IITTVWGVGYKIEA"
     gene            3702..4799
                     /locus_tag="PLN77_04530"
     CDS             3702..4799
                     /locus_tag="PLN77_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM43579.1"
                     /translation="MVFDLIVSALVTVLVVTLTDGILTFFGNGLMNIGYRSAWIGPGG
                     LRPSVRTMILTAAALLSFCLTFYFLMRETIRYIGKILEKVKEISAGDFDHPIEVKGND
                     EFSVMAENLNTMQQNIKEIMERERIAEHTKNDLVSSVAHDLRTPLTSIIGYLGWVRTR
                     TDLDEEMRNHYIDIAYQKALRLEQLTNELFGFVKLEHKEMQLHLGKLDLVQLMEQMLD
                     EMTPSFVKNELQVEFLHAEHAIFLEADGELLARLFGNLLNNAVKYGKEGKQVRVSMEK
                     EGEYVVTRVINYGRIIPKQELDHIFQKFYRTEQSRSRDTGGTGLGLAIVQQIAELHHG
                     QVRVKSDLQGTVFEVELPVGMPKGKKEDEKR"
     gene            4796..>7223
                     /locus_tag="PLN77_04535"
     CDS             4796..>7223
                     /locus_tag="PLN77_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43580.1"
                     /translation="MNRKRRSKICCLSGILLLILLLAGVQRVSAMTTRTELYQNTPSD
                     GGRSGGKEKQLLANSYAGEYQIDFSYGFEGNYQDGNHVPIRVTITNESAVEFEGYFVF
                     QIKNAAESIYRGNSGEGILSKLFPGVKRSGDGRNSLFTYRFPIYIPANGTVTKTFSAG
                     LLVENTSFCVLRLENSQRDTVYSREETISSEGFFQSKVEVCVVEEETNYGQQLNGVTL
                     NDGIYEVEAASVRAEELVGLAESYSPDVLILLEQSKEELDETIQKEIDLWQAEGGLLI
                     TFSGNETFAESQRSAFKEAPVDFLKNILDEETTSRLLAKRNMNYQKYYTISDLLENTK
                     IRNRPNTVLYGGLILVYLVLAGPGLYFFLKKTGKRQYLWGAICACSAVFVMLISLFGQ
                     STRLKAPVLTYVREIWQYDTYQKDYINFCAQAPYNASYELYLDPSYDLVTYNRMDYMS
                     NSTAQPETEDAEYEKTEISFGEQKNRAEISNQAAFALNEFGMQRMETLDEGKGLKGTF
                     HFWDQKISGTVENKTGYDLESCVIAVPGYCALIGDIKNGETITLDGIEADSVREFGNW
                     SAAEDLPEIEKNYLDGVIYNHMPNRSDNCLFFGKLAGNDDTFQLDSGYEAYGISYYYQ
                     EVSVDMKEAGVVYCPYAQEYSGWDGGNTVSFEMGPNSGGISEEETEVTYYLNGVFENN
                     NIWCLYGMYAKGVMQPDSEGIYDPAFWPYAMTALEDKIQAAETGGGGKRGTILSLAFE
                     KARTEDGTKIRNFNGIIELYNFQTGTYDEISDWEIDFASDPALEAQYVNADNQIRVRY
                     RLSEEEKGEEY"
     assembly_gap    7224..7238
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7338..8303
                     /locus_tag="PLN77_04540"
     CDS             7338..8303
                     /locus_tag="PLN77_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43581.1"
                     /translation="MLKIEKLTKKYGKTYALNGLDMEVKKGDIYGFVGPNGAGKTTTL
                     RIISGLLKSDGGDLWLDGVRTTKNILKQKSLIGFVPDFFGVYENLSVIEYLEFFAASY
                     GIYGRAGTVRAREVLEMVELSGKEEQMVDSLSRGIQQRLCLARAMIHKPKLLVLDEPN
                     SGLDPRSRKDFQLLLQRLAREDYTILISSHILSELADLCTSIGVINGGVMVQQGKLEN
                     IMLAIDSSNPLRITVLNQVPKALELLRQDPQVSRLSVDENKIAAWFSGSREEEAYLLQ
                     KLVDAGILVVSFAREHNSLESLFFHLTGETEQKSRNTETFGNTIG"
     gene            8318..9193
                     /locus_tag="PLN77_04545"
     CDS             8318..9193
                     /locus_tag="PLN77_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM43582.1"
                     /translation="MRLNPVFEKEMKRNSRSSRISWIIFVCNLLLAVVAFVCYFGESS
                     QLGYLAPVSYSVPMNCYILMTYLLFILVVLSVPAVAGGSISLEREKKTLDVLLTTNLN
                     PWRIITGKLEASLGVVLILTVSALPVLSLVVVFGGIGLGGLLLMAGGLLLTGIFVGSI
                     GIFCSVVFKRTTLATVLSYVIVVFLVVGICACTGLAYYAGLLQQEMTAAYQQIDVGGV
                     IYLLLFNPFTSFAGIISRQLGNGREMEQLCYLLGNYGQNPLIHYLPEASAVLQLLISA
                     VLLVTAGRKLNPLNK"
     gene            9211..9969
                     /locus_tag="PLN77_04550"
     CDS             9211..9969
                     /locus_tag="PLN77_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRM43583.1"
                     /translation="MKPFCFSLSGIYESEGYAWEQAGKIWDLRELRGTDGYLDPETEQ
                     FLEAELGRKKETKELPRIRLLDSGNYHYMSKLLLGLEKEDLFLAVFDHHTDMQPPALL
                     PVLSCGSWIRDAAGAYQNIKGICVIGPPEASVRETEAMEHVWFVTQEELDDGSGAAKI
                     KEIFASHAGTFPVYLSVDKDILSKEELDTNWDQGNVRVDELLSLAKDCLAGRKVLAVD
                     LCGEPSANAPEAECSAASLVNQKLYGALAAKIED"
     gene            10049..10360
                     /locus_tag="PLN77_04555"
     CDS             10049..10360
                     /locus_tag="PLN77_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HRM43584.1"
                     /translation="MGECCTHCSECDRKKVRDEKEYKDLIHRLSRIEGQVRGIREMVE
                     SDRYCVDILTQVSAIQSALNSFNKVLLSSHIKSCVVEDIRQGKEGAVDELCKTIQKLM
                     K"
     gene            10377..11594
                     /gene="pepT"
                     /locus_tag="PLN77_04560"
     CDS             10377..11594
                     /gene="pepT"
                     /locus_tag="PLN77_04560"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRM43585.1"
                     /translation="MKAYERLLNYVKVPTPSNEKSGTHPSSECQRVLADQLAEEMKAL
                     GVSDVRVEDKCYVYGKIPATKGYEGKTKLGFIAHMDTVSDFADHAVNPIVHENYDGKD
                     LKLADSGRTLEVSVFPHLPSLAGRTLITSDGTTVLGADDKAGVAEIMTLAEALLAGEI
                     PHGPISIGFTPDEEIGEGPDFFDVPGFDAEFAYTVDGGTEGEIEFENFNAAGAKFTVT
                     GFNIHPGGAKDTMINAIAVASEIQSLLPEAESPRNTEGYEGFYHLTSMSGDVAHAESE
                     YIIRDHDAEKFAAKEENLRRIEKAMNEKYGEGTVKLEIKEQYRNMAEKIRPCYHLIEN
                     AEEAARRAGMEPIVLPIRGGTDGARLSFMGLPCPNIGTGGYACHGPYEHITVEGMEKT
                     MAMLKELVKIYAE"
     gene            complement(11718..11789)
                     /locus_tag="PLN77_04565"
     tRNA            complement(11718..11789)
                     /locus_tag="PLN77_04565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11754..11756),aa:Glu,seq:ctc)
     gene            complement(11842..11913)
                     /locus_tag="PLN77_04570"
     tRNA            complement(11842..11913)
                     /locus_tag="PLN77_04570"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11878..11880),aa:Glu,seq:ctc)
BASE COUNT         3225 a         2574 c         3363 g         2834 t
ORIGIN      
        1 ttacagtctg ccgacgtatc agacgcggtg gaatgccaat gcatttctgc tcacgaccgt
       61 aattccggca attctgatgt ttgtgatcaa cgcggtgctg atttccggcc gtctgcgtct
      121 gtcaccgctg cgttttctgc gccacgattt tgcaaagaaa ggaaaaagcc atgcggtgcg
      181 tcttccgaac ttccggttct tctcgcggtt tcgtctgcgg atcctgctgc agaacaaatc
      241 gagttacttt accctgtttc tcggtatcat atttgccaat attctgttgc tgttcggtat
      301 gatgatgaaa ccgctgctgt cacattacca gacggaggcg gtggaaaata tgctggcaga
      361 ttaccagtat attttaaatg tcccggatcc gatcgatgag gatgatgagt acacgctgat
      421 gggaattgtg cagaaacttc tgacgccatc gctgaagaca aatacggaag gcgtggaaac
      481 gtttgctctt acatcgctga aaaatattcc gaaaaaccgg gagggcgaga gcatttcggt
      541 atatggaatt cagaaagaca gcaaatatgt ttccgctgaa ctgtttgaga acggcgtgac
      601 gatttccgac ggttatgccg aaaaatatgg aatgaaagtt ggtgatacgc tggaactgaa
      661 agagccgtat gaggacaaaa cgtacagctt tgaagtaaaa agcatttatg cctacccggg
      721 agcccttgcg gtatttctgc cgatggatgt cttctgcgag gaattcgatc atccagagga
      781 ctactttaac ggctatttct ccaacgagcc gatcacggac ctggaggaat cgtatatcgc
      841 gacgaaaatt acggaagatg atatgacaaa gatttcacgg cagctcaatg tgtcgatggg
      901 agaaatgttc cagctgatca atgttttctc gattgtactg ttcatgctgc tgatttatct
      961 gctgacgaag ctgatcatcg agaagaatac gacttcgatt tcgatggtaa agattctcgg
     1021 ctacgagaac agggaaattt tgagtctgta tttgacggcg acaacctggg ttgtgatcgt
     1081 ctcaatcggt gccagcctga tcattgcctc cgcgctgatc cgcctgattt atgtcattat
     1141 gctttccgag tacagcggct ggctgacata ctatattgat ccggcaattt acggaaaaat
     1201 gtatctgatg ggccttgcgg cgtatgctgt tatcgctgtg ctgcagttcc gccacatcca
     1261 aaagattccg atggacgagg cactgaaaaa tgtagaataa aaaaagttac aggagaaatc
     1321 ttgacaagcc cggtatattg ctctcaaact tggtgtggat ttttcaactt gtaataaagt
     1381 tcttttcgcc acgcaattat cgaaagcaga taattgcgtg gctttttttg tggcaatgga
     1441 gcaaagtccg tgcctgcacg taagcccacc aacagaaaag atcacaaatg gaggtaataa
     1501 tgaacgaaaa ctttatgaaa gaaaagccag tatttccact gctggtttcg atggcgcttc
     1561 caatggtaat ctcgatgctg gtcaattcct tatataacat tgtggacagc ttctttgtcg
     1621 cacagatcag cgaagatgcc atgacggcgc tctccctggt ttatccggta cagaattttg
     1681 taaatgccgt tgccatcggt tttggaatcg gactgaatgc ggtgatttcg tattatctgg
     1741 gagcgggtga caaaaaggca gccggctgtg ctgcaacgca gggtcttgcc cttaccatgc
     1801 tgcatgcggt tattttaacg atcggaagcc ttctggtcat cgagcgtttc ctgcggatgt
     1861 tttcttcttc cgaaactgtc atcgatcttg gtgtccggta ttcacggatt atctttctgt
     1921 ttaccatagc aatcatggca aacctttact ttgaaaaggt ttttcaggca gtcggccgga
     1981 tgaaagtgac gatgaccggt atgatgatcg gctgtattat taacatcatt ctggatccgc
     2041 tgattatttt cggaatcgga ccgtttccgc gtcttggaat tgagggtgcg gcgcttgcaa
     2101 ccggtatcgg tcaggtgaca ccggtcgtga tttactggat catttacaag atccgcccga
     2161 tttcggtcaa aatccgtccg gaatatttaa aagaaggaaa aaatctggtc ggaagactgt
     2221 acgcgatcgg cgtcccggcg atcctgaacc tggcgctgcc gtctcttctg atttccgcac
     2281 tgaatggaat cctggccgtg tattcccaga gctacgttgt cgtgctcggc atttactaca
     2341 agctgcagac gttcatctat ctgacagcaa acggcattgt acagggcatg cgcccgatta
     2401 tcggcttcaa ctacggcgca ggcgagcata agcgtgtgcg gaagatttat gagatcaccc
     2461 tgattatgtg tgcggtgatc atgcttattg gaaccggcat ctgccttgcc attccggaac
     2521 agctgatcgg actgttcagc agcaatccgg agaccatccg tcttggcgga caggcactga
     2581 cacgcatcag cgcgggcttt ttgatttcgg cagtttccat cacatcctcc ggtgcgctgg
     2641 agggactcgg aaaaggcgga tcttccctga tcatttccct ctgccgaaat gtcattgtca
     2701 ttctcccggc agcttacctg ttgtcccatc tgggaggcgg cgttttcgta tggcacgcct
     2761 tctggatcac agaggtcatt tccgcagcgg tttctcttgt ggtataccgc cgcgcagtac
     2821 gaagataaaa aatataagaa gaaaagaaaa gcggcctctt gtggaaggag gcttttcttt
     2881 ttataataga taatacagac gtaagaatta agtaaagatt tttttgaata ttcatggtac
     2941 cattacggta gttaaaaaca gagaggatgg aaacgacgtg gaagaaacaa atattttagt
     3001 tgtggatgat gaaaaagaaa tcgcggatct gctggaaatt tatctgatca gcgatggatt
     3061 tcaggtatta aaagcgtaca gtgcaaaaga gggactccgc attctggagg agaaaaaaat
     3121 cgacctggtg ctgctcgatg tgatgatgcc ggatatggac ggtattgcga tgtgccgccg
     3181 catccgggag acgaacaaca ttccgatcat catggtaagt gccaaaacgc aggatatcga
     3241 caagatcgtc ggtctgtcaa cgggagcaga cgattacgtg gcaaaaccgt tcaatccgct
     3301 ggagctgatg gcgcgtgtaa aatcccagct ccgccgctac cgcgaactga atccgtcgaa
     3361 gccgcacgag cgccaggaag tgatttcctt aaagcatctg gaaatcaaca aggtaaccca
     3421 tcaggttgtc tgctacggag aaagcatcaa gctgacaccg atcgaatttg atattctgta
     3481 tctgctggcc tccaacccgg gccgcgtgtt cggtacagac gaaattttcg agcgcgtctg
     3541 gcatgaaaaa ttctatgagg cgaacaacac ggttatggta catatccgcc gtctgcgcgg
     3601 aaaaatgaaa gacgacacaa gagaagataa gattattaca acagtctggg gagtgggata
     3661 caaaattgaa gcctgatttt ataaaaaact tcagccagag gatggtcttt gatctgattg
     3721 taagtgcact tgtcacggtg cttgtagtga cgcttaccga cgggattctg acctttttcg
     3781 gcaatggact gatgaacatc ggttaccgga gcgcctggat cggaccggga ggcctccgcc
     3841 catcggtgcg gacgatgatt ctgacggcgg ctgcgctgct ttcgttctgc ctgacctttt
     3901 atttcctgat gcgcgaaacc atccgataca ttgggaagat tctggaaaag gtaaaggaga
     3961 tatcagcagg ggattttgat catccgatcg aggtaaaggg aaatgacgag ttttctgtga
     4021 tggcggagaa cttaaatacg atgcagcaga atatcaagga aattatggaa cgggaacgga
     4081 tcgcggagca tacgaaaaac gatctcgtct catccgtggc acatgacctc cgtacgccgc
     4141 tgacttcgat tatcggctat ctcggctggg tgcgcacgcg gacggatctg gatgaggaga
     4201 tgagaaatca ttatatcgat attgcctatc agaaagcact ccgtctggag cagctcacga
     4261 acgagctgtt tggattcgtc aagctggagc acaaagagat gcagctgcat ctcggaaaac
     4321 ttgatctggt gcagctgatg gagcagatgc tcgacgagat gacgccgtct tttgtgaaaa
     4381 atgagcttca ggtggaattt ctgcatgcgg aacatgcgat ttttctcgag gcggacggtg
     4441 agctgctggc acggctcttt ggaaatctgc tgaacaatgc tgtcaaatac ggaaaagagg
     4501 gaaaacaggt ccgcgtctca atggaaaaag agggcgaata tgtggtcacg cgtgtcatta
     4561 actacggacg gatcattcca aaacaggaac tggatcatat tttccagaaa ttttaccgga
     4621 cagagcagtc gaggagccgc gataccggcg ggacgggact tggacttgcg attgtacagc
     4681 agattgcgga actgcatcac ggacaggtgc gggtaaaaag tgatctgcag ggaaccgtct
     4741 ttgaggtgga actgccggtc ggaatgccaa aggggaagaa agaggacgaa aagcgatgaa
     4801 caggaaaaga agaagcaaaa tctgctgtct gtccggtatt ttgctgctga ttctgcttct
     4861 ggcgggagtt cagagggtgt ctgcgatgac aacgcggaca gaactttatc agaacacgcc
     4921 gtcggatggc ggaagaagcg gcgggaaaga aaagcagctt cttgccaaca gctacgcagg
     4981 ggaataccag attgattttt cttatggatt cgaaggaaac tatcaggatg ggaatcacgt
     5041 accgattcgt gtgacaatca caaatgagag cgctgttgaa tttgaaggat actttgtttt
     5101 tcagataaaa aacgccgcgg aatcgattta ccgcggaaac agtggagaag gcattctctc
     5161 aaaattgttt ccgggggtca agcggtcggg cgatggaaga aacagccttt ttacgtaccg
     5221 ttttccaatc tatattccgg cgaacggaac cgttacaaag actttttctg cagggcttct
     5281 ggtggaaaat acgtcttttt gtgtcctgcg cctggagaac agccagaggg acacggtgta
     5341 cagtcgggaa gaaacgattt cgagtgaagg cttcttccag tccaaggtgg aagtctgcgt
     5401 tgtggaagaa gaaactaatt acgggcagca gctgaatgga gttacgctga acgacgggat
     5461 ttatgaggtg gaagctgcat ctgtccgggc agaggagctt gtcgggcttg cggagtcgta
     5521 ttcgccggat gtgctgatcc ttctggagca gtccaaagaa gaactggatg agacaatcca
     5581 gaaagaaatt gatctctggc aggcagaggg aggtcttctg attacctttt cgggaaacga
     5641 gacatttgcg gaaagccagc gcagtgcgtt taaagaagcg ccggtagatt ttctgaaaaa
     5701 tattctggac gaagagacca ccagccgcct tctggcaaaa cgaaatatga attatcagaa
     5761 atactatacg atttccgatc ttctggaaaa cactaagatc cggaaccgtc cgaatacggt
     5821 gctgtacggc ggcctgattc tggtctatct ggttctggca gggccgggat tgtatttctt
     5881 tttgaaaaaa acaggaaaac gtcagtatct ctggggggcg atctgtgcct gctcggccgt
     5941 ctttgttatg ctgatcagtc tgtttggcca gagcacgagg cttaaggccc cggtgctgac
     6001 gtatgtaaga gaaatctggc agtacgatac ataccagaaa gattacatta atttctgtgc
     6061 acaggcgccg tacaatgctt cctatgagct gtatctggat ccgtcctacg atctggtgac
     6121 gtacaaccgt atggactata tgtccaacag cacggcacag ccggaaacgg aggatgcgga
     6181 atatgaaaag acggagatca gtttcggaga acagaaaaac agggcagaaa tttccaatca
     6241 ggctgcattt gccctgaatg agttcggcat gcagcggatg gaaacgctgg atgaaggcaa
     6301 aggactgaaa ggcacgtttc atttctggga tcagaagatc agcggaaccg tggaaaataa
     6361 gacaggctat gatctggaga gctgtgtgat cgccgttcca ggttactgtg ccctcattgg
     6421 agacatcaaa aacggggaga cgatcacgct ggatggaata gaagcggaca gcgtgcggga
     6481 gttcggaaat tggagtgcgg cagaagatct gccggagata gagaaaaatt atctggacgg
     6541 tgtcatctat aatcacatgc cgaaccggag tgacaactgc cttttctttg gaaagctggc
     6601 aggcaatgac gatacgttcc agcttgacag cggatacgaa gcgtatggaa tctcgtatta
     6661 ctatcaggaa gtgtctgtgg acatgaaaga ggccggcgtt gtgtactgtc cgtatgcgca
     6721 ggagtacagc ggatgggacg gcggaaacac ggtaagcttt gaaatgggac cgaattccgg
     6781 cggaatctcg gaagaggaaa cagaagtaac gtattatctg aacggtgtct ttgaaaacaa
     6841 caacatctgg tgtctgtatg gaatgtatgc taaaggcgtg atgcagccgg attccgaggg
     6901 aatctatgat cctgcattct ggccgtatgc gatgacggcg ttagaagata agatccaggc
     6961 ggcagaaacc ggcgggggag gaaaacgggg aaccattctt tcacttgctt tcgagaaagc
     7021 ccgcacggag gatgggacca agatccggaa tttcaatgga atcattgagc tgtacaattt
     7081 tcagacggga acgtatgatg aaatttccga ctgggagatc gattttgcga gtgatccggc
     7141 gctggaggcg cagtatgtga atgcggacaa ccagatccgt gtgcggtacc gtctttccga
     7201 agaagaaaag ggagaagagt atcnnnnnnn nnnnnnnnat tatggatact atgaagcacc
     7261 gaatctgatc gtaaaggcga aagagatccc accggagcaa tatctggaga acctgttagc
     7321 ggaggagcca aatgccgata ctgaaaattg agaaactgac caaaaaatac ggaaaaacat
     7381 atgccctgaa cggtcttgat atggaagtaa aaaaaggcga tatttacggc tttgtcggac
     7441 cgaacggtgc gggaaaaaca acgacgctgc gcattatttc agggctttta aagagcgacg
     7501 gcggcgatct gtggctggac ggcgtgcgga cgacaaaaaa tattctgaag cagaaaagcc
     7561 tgatcggctt tgtgcctgat ttctttggcg tctatgaaaa cttaagcgtg atagagtatc
     7621 tggaattttt tgccgcatcg tatggaattt acgggcgggc aggaaccgtc cgtgcgcggg
     7681 aagtgctgga gatggtcgaa ctatcgggaa aagaggagca gatggtggat tcgctctcaa
     7741 ggggcataca gcagcggctc tgtctggcgc gcgcgatgat ccataaaccg aagcttctgg
     7801 tgctggatga accgaattcc ggtctggatc cgcgttcgag aaaggatttt cagcttctcc
     7861 tgcagcgatt ggcgcgggag gattatacga ttctgatcag ctcccatatt ctttcggaat
     7921 tagcagatct gtgcaccagc atcggtgtca tcaacggagg tgtcatggta cagcagggaa
     7981 agctggaaaa cattatgctt gcgatcgaca gctccaatcc gctgcgcatc actgtgctga
     8041 accaggtgcc aaaggcgctg gaactattgc ggcaggatcc gcaggtcagc cgcctttctg
     8101 tggatgaaaa caagattgcg gcctggtttt cgggaagccg ggaggaggag gcgtatctgc
     8161 tgcagaaact ggtggatgcc ggaatccttg tggtttcgtt tgcacgggaa cacaacagcc
     8221 tggagtccct gttcttccat ctgaccgggg aaacggaaca gaagagccga aacacagaaa
     8281 cgttcggaaa cacgatcgga taaggaggga acgaaacgtg cggttaaatc ctgtatttga
     8341 aaaagaaatg aagcggaact cgcggtcatc gcggatttcc tggattattt ttgtctgcaa
     8401 tctgctgctt gccgtggttg cgtttgtctg ctatttcggg gagagcagcc agcttggcta
     8461 tctggcaccg gtcagttaca gcgttccgat gaactgctat attctgatga cgtatcttct
     8521 gtttattctc gtcgtccttt cagttccggc ggttgcggga ggaagcattt cgctggagcg
     8581 tgagaaaaaa acgctggatg tgctgctgac gacgaacctg aacccgtgga gaatcattac
     8641 gggaaagctg gaggcatcgc ttggcgttgt tctgatactg acggtttcgg cactgccggt
     8701 gctgtccctt gttgtggtgt tcggcggtat cggccttggc ggactgcttc tgatggcggg
     8761 aggccttctt ctgaccggaa tttttgtcgg cagtatcggc attttctgtt cggttgtttt
     8821 taagcggacc acactggcga ccgttctttc ctatgtcatt gtcgtttttc tggtcgtagg
     8881 aatatgcgcc tgcacggggc ttgcctacta cgcaggactg ttgcagcagg agatgacggc
     8941 agcttatcag cagatcgatg taggaggtgt aatttacctt cttcttttca atccgtttac
     9001 ctcgtttgcg gggatcatca gccgccagct tgggaatgga agagagatgg aacagctctg
     9061 ctatctgctt ggaaattacg gacagaatcc gctgatccat tatctgccgg aggcgagtgc
     9121 cgttctgcag cttctgatat cagccgttct tcttgtaacg gcgggaagaa agctgaatcc
     9181 attaaataaa taacaggagt aaaagagaag atgaaaccat tttgttttag cctttccgga
     9241 atttatgagt ccgaaggata tgcctgggag caggcgggaa agatctggga tctgagagag
     9301 ctccggggaa ctgacggtta tctcgatccg gagacggagc agtttctgga agcggaactt
     9361 ggccgaaaaa aagaaacgaa agagctgccg cggatccgcc ttctggattc cggcaattat
     9421 cactatatga gcaaattgct gctgggactg gaaaaagaag atttgttttt agccgtattc
     9481 gaccaccata ccgatatgca gccgccggcg cttcttccgg tgctctcctg cggttcctgg
     9541 atccgggatg ctgccggggc gtatcaaaac atcaaaggaa tctgtgtgat cggcccgccg
     9601 gaggcgtcgg tgcgggaaac agaagcgatg gagcatgtct ggtttgtgac acaggaagag
     9661 ctcgacgacg gaagcggagc ggcgaagatc aaagagattt ttgcatccca tgcgggaaca
     9721 tttccggtat atctgtcggt cgataaggac attctgtcaa aggaggagct tgatacgaac
     9781 tgggatcagg gaaatgtgcg tgtggatgag ctgctgtccc ttgcaaagga ctgtcttgcg
     9841 gggagaaagg tgctcgcggt cgatctctgc ggagagccgt cggcgaacgc gccggaggcg
     9901 gagtgcagcg cggcaagtct ggtaaaccag aagctgtacg gcgcgcttgc ggcaaaaatc
     9961 gaagattgac aaagccgggg caaaagagta tcataaattc atacccagac ggggtatata
    10021 gagtgacgga acacaaggag gaaacctcat gggtgaatgt tgtacacact gcagtgaatg
    10081 cgaccggaaa aaagtgcggg acgaaaaaga atacaaagat ctgatccacc gtctttcgag
    10141 aattgaaggc caggtgcgcg gaatacggga aatggtggag agcgaccgct actgcgtcga
    10201 tatcctgacc caggtatccg cgatccagtc tgcattaaac agtttcaaca aggtactgct
    10261 ttccagccac atcaaatcct gcgttgtgga ggatatccgg cagggaaaag aaggcgccgt
    10321 ggatgagctc tgcaaaacca tccagaagct gatgaaataa gaaggagaag aagaaaatga
    10381 aagcatatga aagattactg aattatgtta aagtgccgac accgagcaat gagaagagcg
    10441 gaacccatcc gtcttccgag tgtcagagag tcctggcaga ccagcttgca gaagaaatga
    10501 aagcgctcgg tgtatcggat gtccgtgtag aggataaatg ctacgtctat ggaaaaattc
    10561 cggcaacaaa aggatacgaa ggaaagacaa aactgggatt tatcgcacac atggatacgg
    10621 tatctgattt tgcagatcat gcggtaaatc cgatcgtaca tgaaaattat gacggaaagg
    10681 atctgaagct ggctgacagc ggaagaacac tggaggtttc ggtattcccg catcttccat
    10741 ccctggccgg cagaacgctg atcacaagcg atggaacaac ggtgctcggt gcagacgaca
    10801 aggcgggcgt ggcagagatt atgacactgg cagaagccct tcttgccgga gaaatcccgc
    10861 acggtccgat ttccatcgga tttacaccgg atgaggagat cggtgaaggc cctgactttt
    10921 ttgatgttcc aggctttgac gcagagtttg cctataccgt ggacggcggc acagagggag
    10981 aaatcgaatt cgaaaacttc aatgcagccg gagcgaaatt cacggtgacc ggatttaaca
    11041 tccacccagg cggagcaaaa gacacgatga tcaatgcaat cgctgttgca agcgagatcc
    11101 agtctcttct gccggaagca gagtccccga gaaataccga aggatatgaa ggcttctatc
    11161 atctgaccag tatgtctggc gatgtggcac atgccgagtc cgagtacatc atccgtgatc
    11221 acgatgcaga gaaatttgca gcaaaagaag agaatctgcg ccggattgaa aaagcaatga
    11281 atgaaaaata cggcgaggga acggtaaaac tggagatcaa agagcagtac cgcaacatgg
    11341 ctgagaagat ccgtccttgc taccatctga ttgagaatgc cgaagaggca gccagaagag
    11401 ccggcatgga gccgatcgtt cttccgatcc gcggcggcac agacggcgca cgcttaagct
    11461 tcatggggct gccgtgcccg aacatcggaa ccggcggata tgcatgccat ggaccgtatg
    11521 agcacattac cgttgagggg atggaaaaga cgatggcaat gctgaaagaa ctggttaaga
    11581 tctacgcaga ataaaagagt tgtcagaaaa aaggaaaagt ccggcagaaa actgccgggc
    11641 ttttttgctg gcgtttatga taggaagaaa aaaatcagca cagcaaaaaa cggagccatg
    11701 caggctccga ttaaaagcag ttcgtagggg aatcgaaccc ctgttttcgc cgtgagaggg
    11761 cgacgtctta gcctcttgac caacgaaccg tatgatatag aacagaattt ttaaagagaa
    11821 gggattctgc aaaaccttca gcagttcgta ggggaatcga acccctgttt tcgccgtgag
    11881 agggcgacgt cttagcctct tgaccaacga accgtatgat atagaacaga atttttaaag
    11941 agaagggatt ctgcaaaacc ttcagcagtt cgtaggggaa tcgaacccct gttttcgccg
    12001 tgagagggcg a
//
LOCUS       DAOMUF010000052        11958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1245_length_11958_cov_3.682517, whole genome shotgun sequence.
ACCESSION   DAOMUF010000052 DAOMUF010000000
VERSION     DAOMUF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1245_length_11958_cov_3.682517"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLN77_04575"
     CDS             <1..186
                     /locus_tag="PLN77_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRM43586.1"
                     /translation="SMAITKRAMLSRAVSAIRHRTLIINLPGSPKAVEESLGFVLDTL
                     PHALGLLRGTDGECGRS"
     gene            208..1251
                     /gene="selD"
                     /locus_tag="PLN77_04580"
     CDS             208..1251
                     /gene="selD"
                     /locus_tag="PLN77_04580"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRM43587.1"
                     /translation="MEEEKIIFCKSGGCTAKLGAGVLEHILEKLPKGEKDPNLLVGYD
                     SKDDASVYRLTDDLAMVQTLDFFPPVVEDPYTFGKIAAANALSDIYAMGGEVRTALNI
                     VCFPEQMDLNVLGEIMRGGAEKVQEAGGSLSGGHSIADDSVKYGLSVTGTVHPDRIWQ
                     NNTGRVGDLLILTKPLGTGILCAAHRVGEENPGGFQKAVESMMTLNKYASETARKYNI
                     HACTDVTGFSFLGHLHEMMESGVSCHIWADQIPVFPGAVEAAEEFLITAAGQRNRNHL
                     EQHVTFKDIPFGMQEVLFDPQTSGGLLMAVDAVDAEKMQRKLLEQGIPAAIVGKVTEP
                     AGTEIYVTQSRKW"
     gene            1269..1892
                     /gene="yedF"
                     /locus_tag="PLN77_04585"
     CDS             1269..1892
                     /gene="yedF"
                     /locus_tag="PLN77_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HRM43588.1"
                     /translation="MAEMEKKTVNAMGDVCPVPLVKAKNAIAELAGSGKVEVLVDNEI
                     AVQNLEKMAQQKGYGFLVKEKKEKEYHVEFTVSETEPAETEEKTVCLVPAAKKTKLVV
                     LSADHMGEGAEELGKILMKSFLYALTQQDELPDTILCYNGGAKLTCEGSESLEDLKDL
                     AARGVEILTCGTCLNFYGITEKLQVGSVTNMYDIVERMSSADRVIKP"
     gene            1889..3037
                     /locus_tag="PLN77_04590"
     CDS             1889..3037
                     /locus_tag="PLN77_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM43589.1"
                     /translation="MIYLDSAATSLWHPKEAEERMIQALRGCGNAGRGAHDATLDAGR
                     IVFETRMRLAELFHAESPERIAFTCNDTEALNTAIFGLFGPGDHVITTVCEHNSVLRP
                     LYALEKQGLELSVIPADVSGRIDYDQMEAAVRKNTKAFVVTHASNLTGNITDLARVCE
                     IAERHSLRLIVDAAQTAGILPIDVQKSGIDVLCFSGHKGLLGPQGTGGIYVRPELSIR
                     PLKMGGSGIRSYEKEQPAAMPEHLEAGTLNVHGIAGLLGALEYLEKTGIEMIYKRERE
                     LMHLFLEEIQGIPGLTLYGTDDLQQRVPTAALNIGSCDSGYVSDWLYENYGIAVRSGA
                     HCAPLMHEALGTREQGAVRFSFSHVLTEEEVRIAAQALREFAEEEMQQ"
     gene            3095..3331
                     /locus_tag="PLN77_04595"
     CDS             3095..3331
                     /locus_tag="PLN77_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HRM43590.1"
                     /translation="MRQRKETWILTFAGTTQAMQMEQYARAHGLPGRMIPVPREITAG
                     CGLSWKAAPEEGKEILAALQTAGLAYEAEYRVLL"
     gene            3347..3718
                     /locus_tag="PLN77_04600"
     CDS             3347..3718
                     /locus_tag="PLN77_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM43591.1"
                     /translation="MEEKEETLRQKLRYDMRLRVSRTDHAFGPGVAELMEHVEATGSL
                     SKGCKCMQMAYSKGWKILKRAEDDLGIPLVQGNRGGSNGGQTVLTPEGKEFLKRYRKF
                     EQEVRAGADASFQKWFGTEEK"
     gene            3868..4713
                     /gene="yqeB"
                     /locus_tag="PLN77_04605"
     CDS             3868..4713
                     /gene="yqeB"
                     /locus_tag="PLN77_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HRM43592.1"
                     /translation="MKKKDLIVVRGAGDLATGTIHRLKKAGFRLLVLEAEHPAAIRRQ
                     VALSEAVYAGSARVEDVEAVRMDVDLAEKKNRKELLEQEMERIWKKDGVPVLVDPAGL
                     SIAALRPAVVVDAILAKKNLGTTKEMAPLVIALGPGFTAGEDVDVVIETKRGHNLGRV
                     IRSGSAVPNTGIPGIIGGYGKERVMHAQAEGILRNAASIGDIVEARAVIAEIETENGT
                     VPVEASLSGLLRGLIRDGYPVTKGFKIADIDPRKEELQNCFTISDKARCIAGSVLEVI
                     CGELE"
     gene            4743..5768
                     /locus_tag="PLN77_04610"
     CDS             4743..5768
                     /locus_tag="PLN77_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRM43593.1"
                     /translation="MKLMKTEDAVGQVLCHDITQIIKGVTKDAVFRKGHIITEEDIPV
                     LLSVGKDNIYIWEKDDTMLHENEAAQILYDMCKNDHMHPSDVKEGKIELIADCDGLLK
                     VDREKLKKVNSLGEMMIATRHGDTYVKKGDKLAGTRIIPLVIKKEKMETAQAVCSDGP
                     ILTLKPFHKKKFAVLTTGNEVYYHRIEDTFTPVIQEKLAEFGAEMIFHEVYDDDASKI
                     TDGCRRAMEAGADLVFCTGGMSVDPDDKTPLAIKNTGARIVSYGAPVLPGAMFLLAYA
                     ENGTPIVGLPGCVMYSKRTIFDLVLPRLIADDEVTAEELAGLGEGGLCLNCKVCTFPN
                     CGFGKGR"
     gene            5772..6743
                     /gene="moaA"
                     /locus_tag="PLN77_04615"
     CDS             5772..6743
                     /gene="moaA"
                     /locus_tag="PLN77_04615"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRM43594.1"
                     /translation="MKDQYGRTIDYLRISITDACNLRCRYCMPESAAGCALEQAEQNP
                     PLTVEEMLKIVHAAAGLGITHYRITGGEPLVRKECVELVQKIKAVPGTASVGITTNGV
                     RLASCARDLKAAGCDSVNVSLDTVDAAEFAALTGRDALEQVKQGICAAKEAGLLVKLN
                     AVHRKELHAQELIEFAEQEKVPVRFIEVMPVGAGKSYVGPSNEALKTELEQWYGACTP
                     DQEKEGNGPAAYVRYEKLSMPVGFISAIHGKFCGSCNRVRLTSRGFLKLCLCYENGVD
                     LREVLQNGTAADLRDVMAQAILKKPMEHCFERPKDMTEHHLMSGIGG"
     gene            6781..7218
                     /locus_tag="PLN77_04620"
     CDS             6781..7218
                     /locus_tag="PLN77_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRM43595.1"
                     /translation="MGKIIGICISEKRGTAKHEVETAKLVEDWGIEGDAHAGHWHRQV
                     SLLSYEKIEAFRAKGADIPLGAFGENLIVEGYDFRNLPVGTRFSCGDALLEMTQIGKE
                     CHSHCEIFKRMGECIMPREGVFAVVLRGGTIQKGDTIELVTEK"
     gene            7248..8270
                     /locus_tag="PLN77_04625"
     CDS             7248..8270
                     /locus_tag="PLN77_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRM43596.1"
                     /translation="MDELYRKLLAQLEEGQDTVLAFITEGDGSIPRTTGAYMLVGREG
                     RIFGTIGGGNLEYQAVLHAQELVQEKKSEYREYDLGTGEGKGLGMVCGGRVKVCFYFM
                     NGAHGEAESLAQALAAQEQYCSYWIFFALDGTGIRVLEKEAWEMLPAAQEKAGHCRIL
                     ELDGKTYYAEEFCYDGKVYLFGGGHLAQELVPVLHHLDFCCIVLDDREEYVDKALFPD
                     AGQTMLVDFTKLDEILSIRKNDYLVIVTRGHRCDADAEAFALRTGASYIGVVGSRRKT
                     KYVREKLEAQGFTGEQLDSVYAPIGIEIGSETPAEIAISIAAQLIQIRSAKTGKRKGA
                     HPALHT"
     gene            8297..9244
                     /gene="modA"
                     /locus_tag="PLN77_04630"
     CDS             8297..9244
                     /gene="modA"
                     /locus_tag="PLN77_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM43597.1"
                     /translation="MKKKTVSILLMAAMALSLAACGSSNSTDSSSKSSSKAESTSASS
                     ASSEAESASSAKSTADGEVFDDTTVTVFAAKSLNSVMETLISEYNKTQPNVKLVGSYD
                     SSGTLMTQIEEGASCDVFFSAAAKQMDQLEGENLLVEGTRHNIVNNQVCVVTYEGSNT
                     EVTGLENLNKASSIALADGSVPVGKYTREALVNAGILEKADDVSAITTQQVSEALGGV
                     EINECANVGAVTSAVAEGSNEVGTVYYSDTYGLEDRLKVLQVVPYDLTGNVIYPAAQV
                     VNKEADETEQAAAKDFVEFLTSDEAAAIFRNYYFDTEVE"
     gene            9390..10094
                     /gene="modB"
                     /locus_tag="PLN77_04635"
     CDS             9390..10094
                     /gene="modB"
                     /locus_tag="PLN77_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRM43598.1"
                     /translation="MQEILDFLQSLDWSPLVISIKTGIVATILSFFFGIFAARAVMKL
                     RPGARAVIDGFLTLPMILPPTVAGFFLLLIFSLRRPFGAALYENFSIKAVQTWLGCIL
                     AATIIAFPLMYRNTRAAFEQVDTNLIYAARTLGMSEWKIFWKVVIPSAGPGVLSGTVL
                     AFARALGEYGATSMLAGNIPGKTGTVSQKIAMVIQDGDYKTAGFWSVVVLLVGFLAMT
                     AMNLLGNRRKKNQKQW"
     gene            10105..11148
                     /locus_tag="PLN77_04640"
     CDS             10105..11148
                     /locus_tag="PLN77_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM43599.1"
                     /translation="MALEVDITKKLDGFFMHMQFRAEREIFAILGASGCGKSMTLKCI
                     AGIEKPDEGRIVLDGKVLFDSEKKINLPPQKRKVGYMFQDYALFPHMNVVQNIEAGMG
                     KRPDRSRTESLIRQFQLEGLEKKIPGQLSGGQKQRVAMARMLAAEPELILLDEPFSAL
                     DTYLKWQLEQEMYKHLKEAGKPVIFVSHNRDEVYRLCDTVSCMNRGKMEVMEPLKEFF
                     QNPKTRTAAILSGCKNISAAERVDAHTMRAVDWGVTLHVKKREIADDIRAVGIRAHFL
                     ATVERPGEENVFPVNESEIWEDPFEWNISFRKNAECSWLQWKIAKTDWSPEMGIPKEL
                     YLKEEDILLLTDS"
     gene            complement(11240..>11958)
                     /locus_tag="PLN77_04645"
     CDS             complement(11240..>11958)
                     /locus_tag="PLN77_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRM43600.1"
                     /translation="MGQMFYSLSIAMGILITFGSYMKKETAIEDSTRNVEVFDTAIAI
                     MAGLMIIPAVFAFSGGDPDTLQAGPSLMFITIPKVFANMGFGTVIGILFFLLVLFAAL
                     TSSIALTESAVSTFEDELGWGRKKSTVLIGVIMIALGTLSCLGYGPLSSVTILGMQFL
                     DFFDFLTNSVMMPIAAIATCLLVSRVIGVAKIEEEIIHGESRFRRKKIFLVMIQYLCP
                     VFALIILFSSVANAFGWITM"
BASE COUNT         3142 a         2676 c         3763 g         2377 t
ORIGIN      
        1 agcatggcga tcacaaaacg ggcgatgctg agccgggcag tgagtgcaat ccgtcatcgg
       61 acgctgatta tcaatctgcc gggaagcccg aaggcggtgg aagagagtct tggatttgta
      121 cttgataccc tgccgcatgc gctgggactt ctgcgcggaa cggacggaga gtgcggaaga
      181 agctgaaaaa agagggaaca ggtaacgatg gaagaagaaa aaattatttt ctgtaagagc
      241 ggcggctgta cggcaaagct cggggcaggc gtgctcgaac atattttgga gaagctgccg
      301 aaaggggaaa aggatccgaa tctgctggtg ggctatgaca gcaaagacga tgcatccgtc
      361 taccggctga cggacgacct tgcgatggtc cagacgctgg atttctttcc gccggtggtg
      421 gaggatccgt acacgtttgg aaaaattgcg gcggcgaatg ccttaagcga catctatgcg
      481 atgggcggag aggtgcggac agccctgaat atcgtctgct ttccggaaca gatggatctg
      541 aatgtcctcg gagaaatcat gcgcggcggc gcggaaaagg tgcaggaggc gggcggatcg
      601 ctctccggcg gtcattcgat tgcggatgac agtgtaaaat acggcctttc tgtcacggga
      661 accgtgcatc cggaccggat ctggcagaac aatacgggcc gcgtgggtga tctgctcatt
      721 ctgacgaaac cgctcggaac cggcattctc tgtgcggcgc accgcgtcgg ggaggaaaac
      781 ccggggggat ttcaaaaggc agtggaatcg atgatgacgc tgaataagta cgcctcggag
      841 acggcacgga agtataacat tcatgcctgt accgatgtaa cgggctttag tttcctagga
      901 catctgcatg agatgatgga gtcgggcgtg tcctgccata tctgggcgga tcagattccg
      961 gtgttcccgg gggctgtgga agcggcggag gagtttctga tcactgcggc aggccagcgg
     1021 aaccggaatc atctggagca gcatgtgacc tttaaggata taccgttcgg catgcaggag
     1081 gtgctgtttg acccgcagac atcgggaggg cttctgatgg ccgtggatgc cgtggatgca
     1141 gaaaaaatgc agagaaaact gctggagcag gggattccgg cagcaatcgt tggaaaagtc
     1201 acagagccgg caggaaccga aatttatgtg acacagagca ggaaatggta aggcagggag
     1261 gatgaaaaat ggcagagatg gagaaaaaga cagtaaacgc aatgggtgat gtgtgcccgg
     1321 taccgctggt aaaggcaaaa aatgcgattg cagaacttgc gggaagcgga aaagtggagg
     1381 ttctggtgga taatgaaatc gccgtgcaga acctggaaaa aatggcgcag cagaaaggat
     1441 atggctttct ggtaaaagag aagaaagaaa aagagtatca tgtggagttc accgtatcgg
     1501 aaacagagcc ggctgaaaca gaagagaaaa ccgtatgcct cgtaccggcg gcaaagaaaa
     1561 caaaactcgt cgttctttct gcagaccata tgggcgaagg agccgaggag ctgggtaaaa
     1621 ttctgatgaa gagcttcctt tatgcactga cccagcagga tgagctgccg gatacgattc
     1681 tgtgctacaa cggcggtgca aagctgacct gcgagggttc cgagtccctg gaggatctga
     1741 aagatctggc agcgcgcggc gttgaaattc tgacctgtgg aacatgcctg aacttctacg
     1801 gcatcaccga aaagctgcag gtcggcagtg tgaccaacat gtatgacatc gtggagcgca
     1861 tgagcagcgc agaccgcgtg attaagccat gatttacctc gattcggcgg caacgtcgct
     1921 gtggcatcca aaagaagcag aggagcgtat gatccaggca ctgcgcggct gtggaaatgc
     1981 aggccgcggt gcacacgatg cgacactgga tgcgggccgc atcgtctttg agacgagaat
     2041 gcggctggca gagttgtttc acgcggagag tccggagcgg attgcattta cgtgcaacga
     2101 tacggaggca ctgaacacgg caattttcgg actcttcggg ccgggggatc atgtgatcac
     2161 gacagtctgc gagcataatt ccgttctgcg cccgctgtat gcactggaaa aacagggact
     2221 ggagctttcc gtgattccgg cggatgtatc tggaaggatc gattacgatc agatggaagc
     2281 ggcagtgcgg aaaaacacga aagcgtttgt cgttacgcat gcctccaacc tgaccggaaa
     2341 tatcacggat cttgcgcggg tgtgcgaaat tgcggaacgc cattccctgc gtctgatcgt
     2401 cgatgcggcg cagacagcgg ggattctgcc gattgatgta cagaaaagcg ggatcgacgt
     2461 gctctgtttt tccggccaca agggactatt agggccgcag ggaaccggcg gaatctacgt
     2521 ccgtcccgaa ctttcgatcc ggccgctcaa aatgggcgga agcggaatcc gaagctatga
     2581 aaaagaacag cctgcggcga tgccggagca cctggaagcg ggaactttaa atgtccacgg
     2641 aattgcggga cttctgggag cgctggaata tctggagaag acggggattg aaatgattta
     2701 caaaagagag agggagctga tgcacctctt tctggaggaa attcagggaa tcccgggact
     2761 tactttatat ggaacagacg atctgcagca gcgggtacca acggcggcat taaacatcgg
     2821 ctcctgtgat tcgggatatg tcagcgactg gctgtatgag aactatggta ttgcagtccg
     2881 aagcggggca cactgcgcgc cgctgatgca cgaagcactc gggacacggg agcagggagc
     2941 cgtgcggttc agcttttccc atgtactgac ggaagaagaa gtccggattg ccgcgcaggc
     3001 tctgcgggag tttgccgaag aagaaatgca gcagtgagag aggcggcatc cggcatccgg
     3061 gatacaaaag aagctggaaa aaggaagaaa cgatatgaga cagcgaaaag aaacctggat
     3121 tctgacgttt gcggggacaa cgcaggcgat gcagatggag cagtatgcga gagcgcacgg
     3181 cctgccggga aggatgatcc ccgttccgcg ggaaattacg gcgggctgcg gcctttcctg
     3241 gaaagcagcg ccggaagagg gaaaagaaat ccttgcggca cttcagacgg cgggacttgc
     3301 ctatgaggcg gagtaccgtg tgctgctgta gaggtgtgag gtggagatgg aagaaaaaga
     3361 agaaacatta cgacagaagc tgcggtacga catgcggctg cgcgtcagcc ggacggatca
     3421 tgccttcggt cccggtgtgg cggagctgat ggagcatgtg gaggcaaccg gctccctttc
     3481 gaagggctgc aaatgcatgc agatggcgta ctcgaaggga tggaaaatct taaagcgcgc
     3541 cgaggatgat ctcggcatcc cgctggtaca gggcaaccgg ggcggcagca acggcggcca
     3601 gacggtactg accccggagg gaaaagaatt cctgaagcgg taccggaagt ttgaacaaga
     3661 agtccgcgca ggcgccgatg cgtcgtttca gaagtggttt ggaacagaag aaaaataaca
     3721 ttttttacgg ggacacgttg acaaaacggg tcctttcctg tattttatta atggaaaagc
     3781 gttattcttg cacgagaata acgaagcaga ccgcggaaga cggaagaaaa cggcggctgc
     3841 tgaaacgaaa gaaggaagac agcgaagatg aaaaagaaag atctgatcgt ggtacgggga
     3901 gcaggagatc ttgcaaccgg tacgatccat cgtctgaaaa aagcgggctt tcgtctgctg
     3961 gttctggaag cagagcaccc ggcggcgatc cggcggcagg tggctttgag cgaagcggta
     4021 tatgccggca gtgccagggt agaggacgtg gaagccgtgc gcatggacgt ggatctcgca
     4081 gagaaaaaga accggaaaga acttctggaa caggaaatgg aacggatctg gaagaaagat
     4141 ggtgttccgg tgctcgtgga tccggcggga ctaagcattg cagcacttcg cccggccgtc
     4201 gtggtcgatg cgattctggc gaagaaaaat ctcgggacga caaaagagat ggcgccgctt
     4261 gtgattgccc tcggaccggg ctttacggca ggagaagatg tggatgttgt aattgagacg
     4321 aagcgcggtc ataatcttgg acgcgtgatc cgaagcggaa gcgcggttcc gaacactggc
     4381 attccgggca tcatcggcgg gtacggaaaa gagcgtgtga tgcatgcaca ggcggaaggt
     4441 attctgcgga atgcggcttc gatcggagac attgtggaag ccagggctgt gattgcagag
     4501 attgagacag aaaacgggac ggtacctgtg gaagcatctc tctccggtct gctgcgcggg
     4561 ctgatccgcg acggctatcc ggtgacgaag ggatttaaga ttgcggacat cgatccgcgg
     4621 aaagaagaac tgcagaactg cttcacgatt tccgataagg cgcggtgcat cgccggaagc
     4681 gtgctggaag tgatctgcgg ggaactggaa tagattcaaa tagaaaaagt gaggcgtttc
     4741 agatgaaact gatgaaaaca gaagacgcgg taggccaggt gctctgccac gatattacac
     4801 agattatcaa aggtgtgaca aaagatgcgg tattccgcaa aggccacatc attacagaag
     4861 aagatattcc ggtgctgtta agtgtcggaa aggacaacat ttacatctgg gaaaaggacg
     4921 atacaatgct gcatgaaaat gaggcggcac agattcttta cgatatgtgt aaaaacgatc
     4981 acatgcatcc gtccgatgtc aaagaaggaa aaattgaact gatcgcggac tgcgacggtc
     5041 ttttgaaagt ggaccgcgag aagctgaaaa aagtaaacag cctcggcgag atgatgattg
     5101 cgacgcgcca cggtgataca tacgtgaaaa aaggcgacaa actggcggga acgagaatta
     5161 ttccgctcgt catcaaaaaa gagaagatgg agacggcaca ggcagtctgt tccgacgggc
     5221 cgatcctgac cttaaagccg ttccacaaga aaaaatttgc ggttctgaca acgggaaatg
     5281 aagtgtatta ccatcggatc gaggacacct tcacaccggt tatccaggaa aagctggcag
     5341 aattcggcgc ggagatgatc tttcatgagg tttacgatga tgatgcatcg aagatcacag
     5401 acggatgccg ccgcgcgatg gaagcgggtg ccgatcttgt cttctgcacc ggaggaatga
     5461 gtgtggatcc ggacgacaag acgccgctgg cgatcaaaaa taccggagca cgcatcgttt
     5521 cctacggcgc gccggtgctg ccgggagcca tgttccttct ggcgtatgcc gaaaacggaa
     5581 caccgatcgt cggcctgccg ggatgcgtca tgtattcgaa acggacgatt ttcgatcttg
     5641 tcctgccgcg cctcattgca gatgatgagg tgacagcgga ggagctggcc ggtcttggcg
     5701 agggcggtct ctgcctgaac tgcaaggtct gcaccttccc gaactgcggt tttggaaaag
     5761 gaagataaac gatgaaggat cagtatggca gaacgataga ttacctgcgg atttccatca
     5821 cggacgcgtg caatttgaga tgccggtact gtatgccgga gagtgcggcg ggctgtgcac
     5881 tggagcaggc ggaacagaat ccgccgctta ccgtcgaaga gatgctgaag attgtgcacg
     5941 ccgcggctgg gcttggcatc acgcattacc ggattaccgg aggggaaccg ctggtaagaa
     6001 aagagtgcgt ggagctggtg cagaaaataa aagcagtgcc gggcaccgca tccgttggga
     6061 tcacgacgaa tggcgtgcgc ctggcatcgt gtgcgaggga tctgaaggcg gccggctgtg
     6121 acagtgtgaa tgtgagcctg gatacagtgg atgcggcaga gtttgcggcg cttaccggac
     6181 gcgatgctct ggagcaggta aagcagggca tctgcgcggc caaagaggcg ggtcttttag
     6241 tgaagctgaa tgcggtgcat cgaaaagaac tgcatgcgca ggaactgatt gaatttgcag
     6301 agcaggaaaa agtcccggtg cggttcatcg aggtgatgcc ggtgggcgcc ggaaaatcgt
     6361 acgtgggtcc gtcgaatgag gcgctgaaaa cagagctgga acagtggtat ggggcatgta
     6421 cgccggacca ggaaaaagaa ggaaacggtc ctgcggctta tgtgcggtac gaaaagctgt
     6481 cgatgcccgt cggctttatc agcgcgatcc acggaaagtt ctgcggaagc tgcaaccgcg
     6541 tacggctgac aagccgcggc tttttaaagc tctgtctctg ctatgagaac ggtgtggatc
     6601 tgcgggaggt tctgcagaac ggaacggcgg cggatctgcg ggatgtgatg gcacaggcaa
     6661 tcttaaaaaa gccgatggaa cactgctttg agcggccgaa ggatatgaca gagcaccacc
     6721 tgatgtccgg catcggcgga tgaaatgacg gcggttcgaa aagaagagga aacaaaagaa
     6781 atgggaaaaa tcataggaat ctgtatcagt gaaaaacggg gaacggcaaa gcacgaggtg
     6841 gaaacggcga agctggtgga agactggggc atcgagggcg atgcacacgc cgggcactgg
     6901 caccgccagg tgagcctttt gtcgtatgaa aaaatagaag ccttccgtgc aaagggggcg
     6961 gatattccgc tcggcgcctt cggggaaaac ctgatcgtgg aaggatatga tttccggaat
     7021 ctgccggtcg gcacgcggtt ttcctgcggg gatgcgctgc ttgagatgac gcagatcgga
     7081 aaggagtgcc acagccactg cgagattttc aaacgcatgg gcgaatgcat catgccgcgg
     7141 gagggcgttt tcgcggtggt gctgcgcggc ggcacgattc aaaagggtga caccatcgaa
     7201 ctggtgacgg aaaagtagac cagagatcag acacaaggag aggaaacatg gacgaacttt
     7261 accggaaact gcttgcacag ctggaagagg gacaggacac ggttcttgcc tttatcaccg
     7321 aaggggacgg ctccattccg cgcacgacgg gcgcctatat gctggtcggc cgggaaggac
     7381 ggattttcgg aactatcggc ggcggaaacc tggaatatca ggcggttctg catgcgcagg
     7441 agctggtgca ggagaagaaa agtgaataca gagaatacga tctcggaacc ggagagggga
     7501 aggggcttgg catggtctgc ggcggcagag tgaaggtgtg cttctatttt atgaatggag
     7561 cgcacggcga agcagaaagt ctggcgcagg cgcttgcggc gcaggagcag tactgcagtt
     7621 actggatttt ttttgcgctg gacggaaccg gcatccgcgt gctggaaaaa gaagcgtggg
     7681 aaatgcttcc ggcggcacag gaaaaagcgg gccactgccg gattctggaa ctggacggga
     7741 aaacgtatta cgccgaggaa ttctgctacg acggaaaggt ttatctgttc ggcggcggcc
     7801 atctggccca ggaactcgtg ccggtgctgc atcatctgga tttctgctgt atcgtactcg
     7861 atgaccggga ggaatatgtg gacaaagcat tgttcccgga cgccgggcag accatgctgg
     7921 tggattttac gaaactggac gagattcttt cgatccggaa aaatgattat ctcgtgattg
     7981 tgacacgcgg ccaccggtgc gatgcggatg ccgaggcgtt tgcgcttcgc accggcgcgt
     8041 cgtacatcgg tgtggtggga agccgcagaa agacaaagta tgtgcgcgaa aaactggaag
     8101 cgcagggttt taccggagaa cagctcgaca gtgtctatgc cccgatcgga atcgagatcg
     8161 gaagcgagac gccggcggag attgcaatca gcattgccgc acagctcatt cagatccggt
     8221 cagcaaaaac cggaaaacga aaaggtgcac atccggcgct tcatacgtaa atatacaaaa
     8281 tgaggagtca attatcatga aaaagaaaac agtatccatt cttctgatgg cagctatggc
     8341 actttctctt gccgcatgcg gatcttccaa ttccacagat tcttccagta agagcagctc
     8401 caaggcagag agcacatctg catccagcgc ttcttcggaa gcagagagcg catccagcgc
     8461 caagagcaca gcagacggcg aagtatttga cgataccact gtcaccgtat ttgccgcaaa
     8521 gagcctgaac tccgtaatgg agacgctgat cagcgagtac aataagaccc agccgaatgt
     8581 caaactggtt ggaagctatg acagctcagg aaccctgatg acacaaatcg aagagggtgc
     8641 atcctgcgac gtattcttct ccgcagcagc aaaacagatg gatcagcttg agggtgagaa
     8701 tctcctggtt gagggaaccc gccacaatat cgtaaacaat caggtctgcg ttgtaaccta
     8761 tgagggaagc aatacagaag ttacgggcct ggagaacctg aacaaggcat ccagcatcgc
     8821 actggcagac ggttccgttc cggttggaaa atacacaaga gaagcactgg tcaatgcagg
     8881 cattctggag aaagcagacg acgtatcggc catcacaacc cagcaggttt ccgaagcgct
     8941 cggcggcgtg gaaatcaatg agtgtgcaaa cgtaggagcc gttacttccg cagttgcaga
     9001 gggatccaac gaggttggta ccgtttatta ttccgatacc tacggtctgg aggaccgtct
     9061 gaaggttctg caggtcgttc cgtacgatct gaccggaaat gtcatctacc cggcagcaca
     9121 ggttgtcaac aaagaggcag atgagaccga gcaggcagca gcgaaagact ttgtagagtt
     9181 tctgacttcc gatgaggcag ccgccatctt ccggaattat tactttgata cagaggtaga
     9241 ataaaaagaa gatcccacaa aaaacaacag ggatttgaaa tcaccgtccg aaaaagaaag
     9301 atttcgtttc tgcggacggt ggttttgcat cagagaaaat ccgggcagga tgcgtgatgc
     9361 aggactgccg tggatagaaa ggaaatgtta tgcaggaaat actggatttt ttacagtcac
     9421 tggactggag cccgcttgtc atttcgatca agaccggtat tgtggcgacg attctgtcct
     9481 ttttctttgg tatttttgcc gcgcgtgcgg tgatgaagct gcgtcccggc gcgcgtgccg
     9541 tgattgacgg ctttctgaca cttccgatga ttctgccgcc gaccgttgca ggctttttcc
     9601 tgcttctgat tttcagcctc cgccgtccgt ttggcgctgc actgtatgag aatttcagta
     9661 tcaaggcagt acagacatgg cttggctgta ttctggcggc aacgattatc gcgtttccgc
     9721 tgatgtaccg caatacgaga gcggcatttg agcaggtgga tacgaacctg atctatgcgg
     9781 cgcggacgct tggtatgtcg gagtggaaga ttttctggaa agtggtcatc ccatcggcag
     9841 gtcccggcgt gctctccggt acggtgctgg catttgcaag ggcgctcggt gagtacggcg
     9901 cgacgtcgat gctggccgga aatattccgg gaaagacggg taccgtttcc cagaaaatcg
     9961 caatggtgat tcaggacggg gattataaaa cggcgggttt ttggtctgtt gtcgtactgc
    10021 tcgtcggatt ccttgcgatg acggcaatga accttctcgg taaccggaga aagaaaaatc
    10081 agaaacagtg gtagaggata aagaatggcg cttgaagtag atattacgaa aaagctggac
    10141 ggatttttta tgcatatgca gttccgggcg gagcgggaga tttttgcgat tctcggcgcg
    10201 tcgggctgtg gaaaaagcat gaccttaaag tgtatcgccg gaatcgaaaa gccggatgaa
    10261 gggcggattg tccttgacgg caaggtgctg tttgattcgg agaaaaaaat caatctgccg
    10321 ccgcagaagc ggaaagtcgg ctatatgttc caggattacg ccctgtttcc gcatatgaat
    10381 gttgtgcaga atattgaggc aggcatggga aaacggccgg accggagccg gacggaatcg
    10441 ttgatccgcc agtttcagct ggaagggctg gagaaaaaga tcccgggaca gctttccggc
    10501 ggacagaaac agcgcgtcgc gatggcacgg atgctcgcag cagagccgga gctgattctg
    10561 ctcgacgagc cgttttcagc gctcgatacc tatctgaaat ggcagttgga gcaggagatg
    10621 tacaaacatc tgaaggaggc cggaaaaccg gtcatcttcg tatcacacaa ccgcgatgag
    10681 gtgtaccgcc tctgcgatac ggtcagctgt atgaaccggg gaaagatgga agtcatggaa
    10741 ccgctgaagg aatttttcca gaatccgaag acgcgcacag cggcaatttt gtcgggctgc
    10801 aagaacattt cggcggccga gcgggtggat gcgcacacga tgcgggccgt ggactggggc
    10861 gtgacgctgc acgtaaagaa gcgggaaatt gcagacgata tccgcgcagt cggcatccgg
    10921 gcacattttc tggccacagt agaaaggccg ggtgaggaaa atgtatttcc ggtcaacgaa
    10981 tccgaaatct gggaagatcc gtttgaatgg aacatttcgt tccggaagaa tgccgaatgc
    11041 agctggctgc agtggaagat cgcaaaaaca gactggagcc cggaaatggg gattccaaag
    11101 gagctgtatc tgaaggagga agatattctg ctgctgacgg attcctgatg ctggtgtgac
    11161 agatttggat aaaaaaagat tgttttcgaa atcgtgatcg tgtttcgaaa acaatctttt
    11221 ttcatcgttt acagattcat tacatggtga tccagccgaa tgcatttgcc acggagctga
    11281 acagaatgat cagtgcaaac accgggcaga gatactggat catgacaaga aagattttct
    11341 ttctgcggaa gcggctctca ccgtggatga tttcttcctc gattttcgcc acaccgatga
    11401 ctctcgaaac gagaagacag gtggcaatgg cagcaattgg catcatcacg gagttcgtca
    11461 ggaagtcgaa gaaatccagg aactgcatgc caagaatggt gacggaggaa agcggtccgt
    11521 agccaaggca ggagagcgtt ccgagcgcaa tcatgataac gccgatcaga acggtcgatt
    11581 ttttacggcc ccagccgagc tcatcctcaa aggtggaaac ggcgctttct gtcagtgcga
    11641 tggagctggt cagtgcggca aacagcacca gaaggaaaaa cagaataccg atcacagtgc
    11701 caaagcccat attggcgaat accttcggga tggtgatgaa catcaaggac ggtcctgcct
    11761 gcagggtatc gggatctccg ccggagaagg caaagacggc aggaatgatc atgagacctg
    11821 ccatgattgc aattgcggtg tcgaaaactt cgacgttccg ggtggaatcc tcaatcgcgg
    11881 tctccttttt catgtaggaa ccgaaggtga tcagaatacc catcgcgatc gagagggagt
    11941 agaacatctg tcccatgg
//
LOCUS       DAOMUF010000053        11757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1284_length_11757_cov_4.128525, whole genome shotgun sequence.
ACCESSION   DAOMUF010000053 DAOMUF010000000
VERSION     DAOMUF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1284_length_11757_cov_4.128525"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..150)
                     /locus_tag="PLN77_04650"
     tRNA            complement(77..150)
                     /locus_tag="PLN77_04650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Pro,seq:cgg)
     gene            complement(387..1688)
                     /locus_tag="PLN77_04655"
     CDS             complement(387..1688)
                     /locus_tag="PLN77_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43601.1"
                     /translation="MKKKKLFSLVLATVLTAATVLPCYGASTKEKISNAQAEQAAAQS
                     QLDSIQSRIDELNSKKGQSEEYLSELNQQLDDLQNRLQELQDQYDAKQNELVQIQADL
                     EDAKAKEEEQYEAMKLRIQYMYENSSGNGYMAMLFSSKSISELISRAEYIQKISDFDR
                     DLMQEYEDTVDQVKEKETQVQEEQAAIVELQQQSSDQQEAIQELYAAAYQELRTYSAE
                     LDDAKSSESALVDEINSKADAINDLIRQAKEEEIAAQKKAEEEAAAAAAAQQAAAEAE
                     AEAARKQAAEAEAARVAAAEAANAAAKADTDTSSSSGSSSSDSSASSTTTESPSASDT
                     SSSSSSGTYLGRFKLTGYCSCSICTGQWSGGSTASGTTPTAGRTIAMGGVPFGTKLMI
                     NGQVYTVEDRGTAYGHVDIFCSSHSEALSFGVQYADVYQLS"
     gene            complement(1879..3312)
                     /locus_tag="PLN77_04660"
     CDS             complement(1879..3312)
                     /locus_tag="PLN77_04660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRM43602.1"
                     /translation="MAQLPQEFLTRMQDMLGDEFDAFLTSYDAPRTAGLRVNTKKCRT
                     EDFPPLVPGTWKKIPWIPNGYFVPDGMRMGQSPLYAAGVFYLQEPSAMTPASRLPIEP
                     GERVLDLCAAPGGKATELNARLKGTGLLVANDISNARAKALLRNLELFGSENVLVTNE
                     TPAGLADVFPGFFDKILVDAPCSGEGMFRKDEAVIGTWTPERPDFFADLQREITSNAV
                     KMLRPGGLMMYSTCTFAPQEDEGTVSFLLENFPEMELIEMEGYEGFSKGNPVWGNGDP
                     EIEKTVRIWPHKMNGEGHYLALFRKKGEAIPYETEEKPIEKKNKKQKNRKKDRGTEAP
                     GPSKAEKQILSDFLSRMTAPIPVEELEVRAGKVYHSPSLPDGVRNLHFLRNGLYLGEL
                     KKDRFEPSQPFAVTLSADKFKDYMNLKADDERTEKYLHGETISVEPGETASPSGWKLV
                     CVDGFGLGWGKLVNGTLKNKYPVGWRK"
     gene            complement(3485..3570)
                     /locus_tag="PLN77_04665"
     tRNA            complement(3485..3570)
                     /locus_tag="PLN77_04665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3534..3536),aa:Ser,seq:cga)
     gene            complement(3750..4298)
                     /gene="tadA"
                     /locus_tag="PLN77_04670"
     CDS             complement(3750..4298)
                     /gene="tadA"
                     /locus_tag="PLN77_04670"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRM43603.1"
                     /translation="MEEETKLETPEEKAHRKERERFMRQAITQAKKAEKLMEVPIGCV
                     IVYEGKVIARGYNRRNTDKNTLSHAELNAIRKASKKLGDWRLEGCTLYVTLEPCQMCA
                     GAIVQARVSEVVIGSMNPKAGCAGSILNMLEMHEFNHQVKVERGVLKEECSEMLSAFF
                     RRLRVVKKKKKEERKRAEEGKE"
     gene            4349..5587
                     /locus_tag="PLN77_04675"
     CDS             4349..5587
                     /locus_tag="PLN77_04675"
                     /inference="COORDINATES: protein motif:HMM:NF040001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6128 domain-containing protein"
                     /protein_id="HRM43604.1"
                     /translation="MNCSAVSFSTLFTSMQEVFMSDYRRFIAYLYEYPNNRKGGCCGF
                     VRVESQNGFCRMDFQIKSPSLPPETSVTVYGFIRRSGRMYGIPLGNLLAGRSSTSGKL
                     FTHSDAIGQTDVTLDELGGLILLCRQTGVIATQWDDLPIQPEFFAPTLPQEPKTSADN
                     GARPTEEKTAETPEPLDPAVPAQSLPSSDASKAQFDELSTEFSSNSELYPQNSNPEST
                     VSTESTSTNTVNTVPADRITQSSANTLHPASAESVKTAESYPSANPEPPQPIPDSAAQ
                     PLHMTSADFPAAAAVPPSRAALYLQLQDQFPEFHPFPDDDLTDCLRLSLDDLPQLRAA
                     GLSVDANRFVLHGSSSYRHLLLARNRDQQELWYFGVPGVYTPNEQFMASLFGFFHFKQ
                     AMRPSELAPDARFGYWYRTL"
     gene            complement(5596..6291)
                     /locus_tag="PLN77_04680"
     CDS             complement(5596..6291)
                     /locus_tag="PLN77_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRM43605.1"
                     /translation="MVIHGFQKLTLLDYPGKVACTLFSGGCNFRCPFCQNGNLVQHPE
                     WEPVIPEEEIFAVLEKRRGVLDGVCVTGGEPTLDPDLERFVTKIKELGYLVKLDTNGY
                     RPEILKKLVEEKKIDYVAMDIKNAPSRYGETAGVKKIDVMRINESVEFLKSGVVDYEF
                     RTTLCKELHGREEIEAIGKWLSGAKRYFLQNYRESDYVIDPRFSGYAREQLERFCEIL
                     RGKIETVEIRGVD"
     gene            complement(6394..7479)
                     /gene="trpS"
                     /locus_tag="PLN77_04685"
     CDS             complement(6394..7479)
                     /gene="trpS"
                     /locus_tag="PLN77_04685"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRM43606.1"
                     /translation="MGKIILTGDRPTGKLHVGHYVGSLRRRVELQNSGEFDKIYIMIA
                     DAQALTDNADNPEKVRQNILEVALDYLSCGLDPEKSTIFIQSQIPELCELSFYYMNLV
                     TVSRLQRNPTVKSEIQMRNFEASIPVGFFTYPISQAADITAFKATTVPVGEDQMPMIE
                     QTQEIVHKFNTVYGEALVQPKIMLPENDSCRRLPGIDGKAKMSKSLGNCIYLSEEPDE
                     IKKKVMSMYTDPDHIKITDPGKIEGNTVFTYLDAFCQPEHFERYLPDYANLDELKAHY
                     QRGGLGDVKVKKFLNNVLQETLEPIRNRRKELEKDIPAIYEMLKKGSEEAEKVAAQTL
                     ADVKAAMKINYFDDLDLIRSQAERYGK"
     assembly_gap    7525..7624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7694..8458)
                     /locus_tag="PLN77_04690"
     CDS             complement(7694..8458)
                     /locus_tag="PLN77_04690"
                     /inference="COORDINATES: protein motif:HMM:NF037631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HRM43607.1"
                     /translation="MNEEEVLVGRIQDLAEQCYHRDVPSHTEFLTLAEQDLFYRTVNK
                     IPGVRYVLSGGYEAAERKAALFLPSYAEDGDASMLPIAVVRISPLNEKFADALSHRDF
                     LGSLMNLGVERYKLGDILIDGNQAYLICMADMADYICAELKKVRHTSVYCEVEAGLPE
                     ELTEPKTERKEGSVASVRLDAVLSLAFSSSRSKMVPLIEGGQVFINGKLVTSPSASLK
                     EDDLVTVRHMGKFRYVGVQNQTKKGRLYVAVERFVS"
     gene            complement(8525..10669)
                     /locus_tag="PLN77_04695"
     CDS             complement(8525..10669)
                     /locus_tag="PLN77_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRM43608.1"
                     /translation="MDMNQVLAAELNVKPWQVEAAVKLIDEGNTIPFISRYRKEATGS
                     LNDEILRNLYDRLMYLRSLNDRKAVVLASIEEQGKLTAELKKSIEEAATLVVVEDLYR
                     PYRPKRRTRATIAKEKGLEPLANIILLQMTKEPLEKEAEAFVSEEKEVKTAKDAIAGA
                     CDIIAESISDEADYRMEIRRRTEAKGLIVSTAKDEKAESVYENYYEFSEPVSKIAGHR
                     VLALNRGEKEKFLNVKIEAPTEEILRYLEKKIITKENPQTKPVLQATIEDAYNRLIAP
                     AIEREVRNQLTEKAEDGAIKVFGKNLEQLLMQPPIAGQVVLGWDPAFRTGCKLAVVDA
                     TGKVLDTTVVYPTAPTNEKKIAAAKATVKDLIHKYGVTLISVGNGTASRESEQIIVEI
                     LKEIPEKVAYVITNEAGASVYSASKLATEEFPNFDVGQRSAASIARRVQDPLAELVKI
                     DPKSIGVGQYQHDMNQKKLGEALGGVVEDCVNKVGVDLNTASASLLEYISGVSKVIAK
                     NIVAYREENGRFESRKELLKVAKLGPKAFEQCAGFMRITGGKNPLDGTSVHPESYEAV
                     EKLFAKLNMKTEQISEGSTAFFIKDYKKMAEEIGVGEITLRDIIKELQKPGRDPRDEM
                     PRPILRTDVLDMKDLKVGMILKGTVRNVIDFGAFVDIGVHQDGLVHISQMTKRFIKHP
                     LEAVSVGDIVEVKVISLDLAKKRIGLSMLLDK"
     gene            10879..11358
                     /locus_tag="PLN77_04700"
     CDS             10879..11358
                     /locus_tag="PLN77_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43609.1"
                     /translation="MTPENYDPNTNESGDSYQSSHPYGVPEGQRPGPYQHYQQQPPVQ
                     PPRRTGSQALAAASMVMAICSFLFMLNMGSLFFGSLGVIFALLSRGAGRMSGTAKAGL
                     AGCTIGIIVGICMYASFLLFNMVSGGSTNLLERYYNQYMQEYQMTPDNGTTTDGNTI"
     gene            11378..>11757
                     /locus_tag="PLN77_04705"
     CDS             11378..>11757
                     /locus_tag="PLN77_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43610.1"
                     /translation="MNGQKQKFSELKKQAREAMAGRCGTLVGATAINLFLTFSCSLIP
                     TLIFSGTTLYDIIFLNLTLFLFSLLLSLFQAGYLKICLSTLRGEPISVGDMLYAFYHH
                     PDQYVLMFLIINGISSVIALISSIP"
BASE COUNT         2293 a         3424 c         2680 g         3260 t
ORIGIN      
        1 tttcctgttc tctttctgac atacgaaaaa atgccgaagc tcttgattgt caaggctttc
       61 ggcattttca ttccagtcga agcgacggga ttcgaaccca cgacctctgc gtcccgaacg
      121 cagcgctcta ccaaactgag ccacgcttcg attttatttg ttttctgaac tctcgtccgt
      181 caacaatagc tattatatat gatgtctata ggaatgtcaa cacctttttt gaaatttttt
      241 taattttttt tattattgct tcaaaaggta cattttacac gggtttgcgg cctttttggt
      301 gttcttttag aatgacagtt tctgagaaag tcaaaaaaag agatatgccg gaagttctgt
      361 tccggcatat ctcttatctt tgtttctcag ctcagctgat atacatctgc atactgcacg
      421 ccgaagctca gcgcctcgga atggctgctg cagaagatgt ctacgtggcc gtaagcggtt
      481 ccgcggtctt ctaccgtgta aacctggccg ttgatcatca gtttcgttcc gaatggtaca
      541 ccgcccatgg cgatcgttct tcctgcggtc ggtgtggttc cgctcgcggt ggatccgccg
      601 gaccactgtc cggtacagat ggaacaggag caatagcctg tcagtttaaa tcttccaagg
      661 tatgttccgc tggaggagct gctggaagta tctgacgcgg atggactctc cgttgtcgtg
      721 gaggatgcgc tgctgtccga agaactgctt ccggagctgc tgcttgtatc ggtatctgct
      781 ttcgctgcgg catttgctgc ttctgcggct gcgactcttg cggcctctgc ctcggctgcc
      841 tgttttctgg cggcctctgc ctcagcttct gctgcagcct gctgtgcggc ggctgctgcg
      901 gctgcttcct cttctgcttt cttctgcgct gcaatctcct cttctttcgc ctgacggatc
      961 aggtcgttga ttgcatctgc tttgctgttg atttcatcta ccagtgcact ctcgctggat
     1021 tttgcatcgt ccagctctgc ggagtaggta cggagttcct gataagctgc tgcgtacagc
     1081 tcctggatgg cttcctgctg atcgctgctc tgctgctgca gttctacgat cgctgcctgc
     1141 tcttcctgca cctgtgtttc tttttctttt acctggtcta ccgtgtcttc atactcctgc
     1201 ataagatcgc ggtcgaaatc ggaaattttc tgtatgtatt ctgcccggct gatcagctct
     1261 gaaatgcttt tggaagaaaa gagcattgcc atgtatccgt ttccggaaga attttcatac
     1321 atatactgga tacggagctt catcgcctcg tactgctctt cttcttttgc ttttgcatct
     1381 tccagatctg cctgaatctg taccagttcg ttctgcttcg catcatactg atcctgaagc
     1441 tcctgcagac ggttctgcag atcgtccagc tgctggttca gttcggaaag gtattcttct
     1501 gattgtccct ttttggaatt gagttcgtca atcctgctct gtatgctgtc aagctgagac
     1561 tgtgcggcag cctgttctgc ctgagcattt gaaattttct cctttgtact ggcaccatag
     1621 catggcagaa cggttgctgc cgtaagcact gtggccaaaa ccaggctgaa taatttcttt
     1681 tttttcatgt cgtacacctc atacttatta cgttctgttg ctgattgtca ctccaagtta
     1741 ctgcggaaaa tgggcctttt ttcccgccgt actttgccat tataacacaa gtatgtctga
     1801 aatgaaagtc ttttttaatt tctgttaata actttgtaat atttcgacgt ttttcctgca
     1861 aaaactggca tgatttttct actttctcca tccaacaggg tacttatttt tcaaggttcc
     1921 attgaccagt ttgccccatc caaggccgaa tccatcgacg cagaccagct tccagcccga
     1981 cggcgatgcg gtctctcccg gctctacgga aattgtctca ccgtggagat atttttcggt
     2041 acgctcgtca tctgccttta aattcatgta atctttgaac ttgtccgcgg agagtgtcac
     2101 ggcaaacggc tgtgacggct cgaaacggtc ttttttcagt tcaccgagat acagaccgtt
     2161 ccgcaggaaa tgaagattcc gcacgccatc cggcagggac ggggaatggt atacttttcc
     2221 ggcgcggact tccagttcct ccaccgggat cggtgctgtc atccgggaca ggaaatcgga
     2281 aaggatctgt ttttctgctt tcgacggacc cggtgcttcc gttccgcggt cttttttccg
     2341 gtttttctgt tttttgtttt tcttttcgat cggtttttct tctgtctcat aaggaatggc
     2401 ttcgcctttt ttgcggaaca gggcaaggta atggccctcg ccgttcattt tatgcggcca
     2461 gatgcgcacg gtcttttcga tctccggatc gccgtttccc cagactggat tgccttttga
     2521 aaaaccttcg tagccttcca tctcgatcag ttccatttcc gggaagtttt ccagaaggaa
     2581 agaaacggtt ccctcgtcct cctgcggggc gaaggtacac gtcgaataca tcatgaggcc
     2641 gcccggacgc agcattttga cagcattcga ggtgatctcg cgctgcagat ccgcaaagaa
     2701 atccggccgc tccggggtcc aggtgccgat gactgcctcg tctttccgga acatgccctc
     2761 gccggagcac ggtgcatcca cgaggatttt atcgaagaag cccgggaaga cgtctgcaag
     2821 gcctgccggt gtctcattgg tgacaagcac gttttcgctt ccgaacagct ccagattgcg
     2881 cagcagtgct ttggcgcggg cgttgctgat gtcattggcg acgagaaggc cggttccttt
     2941 caggcgcgca ttcagctcgg ttgccttgcc gcccggtgcg gcgcagagat cgagcacacg
     3001 ctcacccggt tcgatcggga ggcgggacgc cggggtcatg gcgcttggct cctgcagata
     3061 gaagacgcct gccgcataca gcggagactg gcccatgcgc atgccgtccg gcacgaaata
     3121 gccgtttgga atccacggaa ttttcttcca cgttcccgga acaagcggcg ggaaatcttc
     3181 ggtccggcat ttttttgtat tcacacgcag cccggctgtg cggggtgcgt cgtaggaagt
     3241 taaaaatgca tcaaattcat cgcccagcat gtcctgcatg cgggtcagaa attcctgtgg
     3301 taactgcgcc atgtattgtc tctccttttt attttgcttt gttgtgcatc ggtccctttt
     3361 cgtgaactgg cgatgctttt tctgtttttg tttcttttgt tttgcatcta tttcataatt
     3421 tctcacagaa aaagagacag ctttttcgat ttttcaatct ttttgctgtc tcttttttgc
     3481 atagcggaga agaagggatt tgaacccttg cgccgtttcc gacctactgg tgttcgaagc
     3541 cagacccttc agcctcttgg gtacttctcc gtattttgtc aaatacatca tatattatag
     3601 tgcttttttc gggattcgtc aatgattttt tcgatggttt ccgatggaaa tacggggatt
     3661 gtatttttta cggaaatatt cgaaagtgca acttactcaa acaaataatc ttcctcaggc
     3721 agcatcccat cccgatgctg cctcttcttt tattctttcc cttcttccgc cctcttcctc
     3781 tcttcttttt tcttcttttt caccacccgc agtctccgga aaaacgcact cagcatctcc
     3841 gagcactctt ctttcagcac gccgcgctcg accttcacct gatggttaaa ctcgtgcatt
     3901 tccagcatat ttaagatcga tcccgcgcag cccgccttcg ggttcatgct gccgatgacg
     3961 acttcgctga ctctcgcctg tacgatcgca ccggcgcaca tctggcacgg ttccagggtg
     4021 acatacaacg tacagccctc cagccgccag tcgccgagct ttttgctggc tttccggatc
     4081 gcattcagtt ccgcgtggga cagcgtgttt ttatccgtat ttctgcggtt atagccccgc
     4141 gcaatcacct ttccctcgta cacaatcaca cagccgatcg gcacttccat cagcttttcc
     4201 gctttcttcg cctgcgtaat cgcctggcgc atgaagcgtt cccgttcttt ccggtgcgct
     4261 ttttcctccg gcgtttccag ctttgtttct tcttccattg tgcacttcct ttcttctctg
     4321 cgtatatttt ctgtccttcc cgcatatcgt gaactgtagc gcagtatctt tttctactct
     4381 ttttacttct atgcaggagg tttttatgtc tgattaccgg cgtttcattg cctatctata
     4441 cgaatacccg aacaaccgca agggcggatg ctgcggcttt gtccgcgtgg agtcgcagaa
     4501 cggcttctgc cgcatggact tccagatcaa atcgccgtcg cttccgccgg agacctcggt
     4561 cacggtctac ggatttatcc gccggtcagg gcggatgtac ggcattccgc tcggaaatct
     4621 ccttgcgggg cgctcttcta cgagtggaaa actcttcacg cactccgatg ccattgggca
     4681 gacggatgtc acgctcgacg agcttggcgg tctgatcctg ctctgccgcc agaccggcgt
     4741 catcgccacc cagtgggacg atcttccgat tcagccggag tttttcgctc ccacgctccc
     4801 gcaggagccc aaaacatccg cagacaatgg tgccagaccg accgaagaga aaactgccga
     4861 aacacccgaa cctctggatc ccgccgtacc tgcgcagtcc ctgccatcat cagacgcttc
     4921 aaaagcacag ttcgatgaac tttccacaga attcagcagc aattccgaac tttatccaca
     4981 aaattccaac ccggaatcca ccgtctccac agaatccaca tcaactaaca cggtaaacac
     5041 cgttcctgca gacaggatca cgcaatcatc cgccaacaca ctccatcccg catccgcgga
     5101 atctgtgaaa accgcagaaa gctatccctc tgccaacccg gaaccgccgc agcccatccc
     5161 ggattctgcc gcccagccgc tacatatgac ttccgccgat tttcccgccg cagcagccgt
     5221 gccgccgtcc cgcgctgctc tctatctcca gcttcaggac cagttcccgg agttccatcc
     5281 gttcccagat gatgatctga ccgactgcct gcgcctctca ctcgacgatc ttccacagct
     5341 ccgcgccgcc ggtctttcgg tggatgcgaa ccgcttcgtc ctccatggct cctcgtccta
     5401 tcgccatctg ctcctcgccc ggaaccgcga ccagcaggaa ctctggtatt tcggcgtccc
     5461 cggcgtctac acgccgaatg agcagttcat ggcaagcctc ttcggctttt tccacttcaa
     5521 gcaggcgatg cgcccctccg agctcgcgcc cgatgcccgc ttcggctact ggtaccgcac
     5581 gctttaaagc gttttttaat ccacgccgcg gatctccacc gtctcaatct ttccgcgcag
     5641 gatctcgcag aaccgctcga gctgctccct cgcatacccc gagaacctcg gatcaatcac
     5701 ataatccgat tcccggtaat tctgcaggaa gtaccgtttt gccccggaaa gccactttcc
     5761 gatcgcttcg atctcttccc ttccatgcag ctctttgcag agcgttgtcc ggaactcata
     5821 gtccaccacg ccgctcttta aaaactcgac cgactcattg atccgcatca catcgatctt
     5881 tttcacgccc gccgtctcgc cgtaccggga cggcgcgttt ttgatgtcca tggcgacgta
     5941 gtcgattttc ttctcttcca ccagcttttt caaaatctcc ggccggtatc cattcgtatc
     6001 gagcttcaca agatatccca gttccttaat tttcgtcacg aaccgctcca gatccggatc
     6061 cagcgtcggc tcaccgcccg tcacgcagac gccgtccagc acgccgcgcc gcttttcgag
     6121 cactgcaaaa atctcctcct ccggaatcac cggctcccat tcgggatgct gcacgagatt
     6181 tccgttctgg cagaacgggc agcggaaatt acatccgccc gaaaacaatg tgcacgccac
     6241 cttgcccggg taatccagca gcgtcagctt ttgaaatcca tgtatgacca tatgctatcc
     6301 tctccgaccg caggactccg aactttcgtc cgccctgcaa cagaaaaagc ggaaccataa
     6361 tgtccgcttt ttccattcat cttctattgg taattatttg ccgtaacgct ctgcctggga
     6421 acggatcagg tccagatcat caaaatagtt gattttcatg gctgctttga catccgccag
     6481 tgtctgagcg gcaacttttt ctgcctcttc gctgcctttt ttcagcatct catagatcgc
     6541 cggaatgtct ttttccagct cttttctgcg gttacggatc ggctctaagg tctcctgcag
     6601 tacattgttc aggaacttct tcactttgac atcgccaagg ccgcctctct ggtaatgtgc
     6661 tttcaactcg tcgagatttg cgtaatccgg cagatagcgc tcgaagtgct ccggctggca
     6721 gaatgcatcg agataggtaa atacggtatt tccctcgatc ttgcccgggt cggtgatctt
     6781 gatgtgatcc gggtcggtgt acatgctcat gacttttttc ttgatctcat ccggctcctc
     6841 ggacaggtaa atgcagtttc caagcgattt actcatcttc gcttttccat cgatacccgg
     6901 cagacgtctg caggaatcat tctccggcag cataatcttc ggctgcacaa gtgcctcgcc
     6961 ataaaccgta ttgaatttgt ggacgatctc ctgtgtctgc tcgatcatcg gcatctgatc
     7021 ctctccaacc ggaacggtcg ttgccttgaa tgcagtgatg tcggctgcct ggctgatcgg
     7081 gtaggtgaag aatccaaccg ggatgctcgc ctcaaagttt ctcatctgga tctccgattt
     7141 tacggttggg tttctctgca gtctggaaac cgtaacgaga ttcatataat agaaagaaag
     7201 ctcgcacagt tccggaatct gagactggat aaagatggtg ctcttttccg gatcaagtcc
     7261 gcaggaaagg taatccagtg ctacctccag aatgttctgg cgcaccttct ccgggttgtc
     7321 cgcattgtcg gtcagcgcct gtgcgtctgc aatcatgatg taaattttat cgaattcacc
     7381 ggagttctgc agctccacgc ggcggcgcag tgaaccaacg taatgtccta catgcagctt
     7441 gccggtcggt cggtcgccgg tcagtataat ttttcccatt ttttcttcct gccttcctga
     7501 tacgtaatct tcgcagtccg cttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnncctcta taataccgtc tttttacaaa cactgtcaat ggtcttttcc tgtaaaatcc
     7681 ctcaaaatat ctattatgag acaaatcgtt caacagccac gtacagcctt ccctttttcg
     7741 tctgattctg cacgccaaca tagcggaatt ttcccatatg gcgcacggtc acgagatcgt
     7801 cctcttttaa tgacgcgctc ggcgacgtca ccagttttcc attgataaac acctggccgc
     7861 cctcgatcag cggcaccatt ttgctccgcg aactgctgaa cgccagcgac agcaccgcat
     7921 cgagccgcac ggaagcgacg gagccttctt tccgctcggt tttcggctcc gtcagctcct
     7981 ccggcagtcc cgcctcgact tcacagtaca cgctcgtatg gcgcactttt ttcagctccg
     8041 cacagatgta gtccgccata tccgccatac agatcagata cgcctggttt ccatcgatca
     8101 gaatgtctcc cagcttatac cgctccacgc cgagattcat cagtgatcct aagaaatcac
     8161 ggtgtgacag cgcatccgca aatttttcat tcagcgggct gatgcgcacg actgcgatcg
     8221 gaagcatcga cgcatcaccg tcctctgcat aggatggcag aaacagcgcc gctttccgct
     8281 ctgccgcctc gtatccgccg gacagcacgt aacgcacgcc cgggatttta tttaccgtgc
     8341 ggtagaacag atcctgctcc gccagcgtca gaaactccgt gtgtgacgga acatccctgt
     8401 gataacactg ctctgccaga tcctgaatcc gtcctaccag cacttcttcc tcattcatgg
     8461 taatccccct ttgatacacc gcgggctgcc cctgttttct ttcgggcagc ccacgtatca
     8521 cactttattt atccagcagc atcgacagcc cgatccgctt cttcgccaga tcaaggctga
     8581 tgaccttcac ttccacgata tcgccgacgc tcacagcctc cagcggatgc ttgatgaagc
     8641 gtttcgtcat ctgggaaatg tggacgagtc cgtcctgatg cacgccgata tcgacgaatg
     8701 caccgaagtc aatgacgttg cgcacggttc cctttaagat catgccgacc ttcaggtcct
     8761 tcatatcgag cacgtcggtg cgcaggatcg ggcgcggcat ctcgtcacgc gggtcacggc
     8821 caggcttctg cagctctttg atgatatcgc gcagcgtaat ctcgccgaca ccgatctcct
     8881 ctgccatctt cttgtaatct ttgatgaaga acgccgtcga tccctcgctg atctgctctg
     8941 tcttcatatt taactttgca aacagcttct cgactgcctc gtagctctcc ggatgaacgc
     9001 ttgtgccgtc cagcggattt ttgccgccgg tgatgcgcat aaagcccgcg cactgctcaa
     9061 acgccttcgg tccgagtttt gccactttca ggagctcttt tctgctctcg aaacggccgt
     9121 tctcctcgcg gtatgccacg atgttttttg ctatcacctt gctgacaccg gaaatgtatt
     9181 ccagcagcga cgcggatgcc gtgttcagat cgacaccgac tttattcacg cagtcctcaa
     9241 cgacaccgcc gagcgcctcg ccgagcttct tctggttcat atcgtgctga tactggccga
     9301 caccgatgga tttcggatcg attttgacaa gctctgccag cggatcctgc acacggcgtg
     9361 cgatggaagc ggcacttcgc tgtccgacat caaagttcgg gaattcctcg gttgccagct
     9421 tgcttgcaga gtaaacggat gctcctgcct cgtttgtgat cacatacgca accttctccg
     9481 ggatctcttt taagatctcg acgatgatct gctcggactc acggcttgca gttccgtttc
     9541 cgacagaaat cagggtgacg ccgtatttgt ggatcagatc ttttacagtg gctttcgctg
     9601 ccgcaatctt cttctcgttc gtcggcgcgg tcggataaac cacggtggta tcgagcactt
     9661 ttccagtcgc atcgacaaca gccagcttgc atcccgtacg gaacgccggg tcccatccaa
     9721 ggacaacctg gcctgcgatc ggcggctgca tcagaagctg ctcaaggttc tttccaaaga
     9781 ccttgatcgc accatcctct gccttttcgg tgagctgatt tctgacctca cgctcgatgg
     9841 ccggagcgat cagacggttg taggcatcct cgatggtcgc ctgcagaacc ggctttgtct
     9901 gcggattctc tttggtgatg atttttttct cgagataacg caggatttct tccgtcggcg
     9961 cctcaatttt gacgttcagg aatttttctt tctcaccgcg gttcagtgcc agcacacggt
    10021 gtccggcaat ttttgatacc ggctcggaaa actcatagta attctcgtaa acggactctg
    10081 ctttctcatc ctttgctgtc gagacgatca ggcccttcgc ctcggttctg cggcggatct
    10141 ccatacggta atcggcctca tcggaaatgc tctcggcgat gatgtcgcag gcaccagcaa
    10201 tggcgtcttt tgccgttttg acttcctttt cttctgaaac aaaagcctct gcctcttttt
    10261 cgagcggctc ttttgtcatc tgcagaagaa tgatattcgc cagcggctcc agtccttttt
    10321 cttttgcgat cgtcgcacgc gtccggcgtt tcgggcggta cgggcggtat aaatcctcta
    10381 caaccacgag agttgcggcc tcttcgatgg attttttcag ttctgcggtc agcttgccct
    10441 gctcctcgat gctcgcaagg acaacggcct tgcggtcatt taaactgcgc aggtacatca
    10501 gacgatcgta cagatttcgc agtatctcat cgtttaagga acctgttgct tcttttcgat
    10561 atcgtgaaat aaaagggatc gtgtttccct cgtcaatcag tttcactgca gcttctacct
    10621 gccacggttt gacgttcagc tcggctgcca atacctgatt catatccata ggtaaattcc
    10681 tcctgttgtc tcttccaaaa cacgctgttc cttctctgtt tttcccgcat aaaaagcggg
    10741 acccttggcg gaacagccac tctattacct tatcaatagg tcttgttttt gtcaacctta
    10801 catgataaaa tctttactat agcttttttg acagactgcg tgtgaaaccg cgcttttttg
    10861 cagaagaaag gacattttat gacaccagaa aattacgatc caaacaccaa tgagtccgga
    10921 gattcctatc agtcctccca tccgtacggc gtgcctgagg gccagcgtcc cggaccgtat
    10981 cagcattacc agcagcagcc tcccgtacag ccgccgcgca ggacgggttc gcaggccctt
    11041 gcggccgctt cgatggtcat ggcgatctgc tcctttttgt tcatgctcaa catgggttcc
    11101 ctgtttttcg gctcgctcgg cgtgatcttc gcccttttaa gccgcggcgc aggacgcatg
    11161 agcggcacgg caaaagccgg tctggcgggc tgtaccatcg gcataattgt cggtatctgc
    11221 atgtatgctt ctttcctgct tttcaacatg gtcagcggcg gcagcaccaa cctgctcgag
    11281 cggtattaca atcagtacat gcaggaatac cagatgacac cggataacgg aacaaccacc
    11341 gacggaaata ccatctaggt accagaaagg atttttcatg aacggacaaa aacagaaatt
    11401 ttcagaactg aaaaagcagg cccgtgaggc catggcaggc cgatgcggca cgctggtggg
    11461 cgcaaccgcg atcaacctgt ttctcacctt ctcctgctcc ctcatcccga cgctgatttt
    11521 ttcaggcaca acgctgtacg atatcatttt cctgaatctg acgctgttcc tgttttccct
    11581 gctcctcagc ctcttccagg caggctactt aaaaatctgc ctctcgacgc tgcgcgggga
    11641 accgatttcc gtcggtgata tgctctacgc tttctatcac cacccggacc agtacgtgct
    11701 gatgttcctt atcatcaacg gcatcagctc cgtgatcgcc ctgatttcca gcattcc
//
LOCUS       DAOMUF010000054        11696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1298_length_11696_cov_3.533459, whole genome shotgun sequence.
ACCESSION   DAOMUF010000054 DAOMUF010000000
VERSION     DAOMUF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1298_length_11696_cov_3.533459"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLN77_04710"
     CDS             complement(<1..232)
                     /locus_tag="PLN77_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HRM43611.1"
                     /translation="MGHLVITIGCEYGAKGNAIGRKIAEDLGIKFYDRELVDQIIEEV
                     GIPKDIMNRVEEGGTIAGKGAEGDVRGSFSKYA"
     gene            complement(396..>673)
                     /locus_tag="PLN77_04715"
     CDS             complement(396..>673)
                     /locus_tag="PLN77_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM43612.1"
                     /translation="GNAKKVGDYFAEKLAKLPHVKEVRHQGLLVGVEFDDTISGVEVK
                     HGCLDRHLLITAIGAHIIRLIPPLIVTEEDCDKAVAIIEETVKALEK"
     assembly_gap    674..683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<684..1619)
                     /locus_tag="PLN77_04720"
     CDS             complement(<684..1619)
                     /locus_tag="PLN77_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM43613.1"
                     /translation="MRLKDTGLTVQDIKDKVNKYMIETYERFDFLAETAEGMYMYDEN
                     GTPYLDFYAGIAVNNAGNRNPKVVAAAKEQLDDIMHTFNYPHTIPQALLAEKVCETIG
                     MDKIFYQNSGTEANEAMIKMARKYGIEKYGPNRYHIVTAKMGFHGRTFGAMSATGQPG
                     NGCQVGFGPMTYGFSYAPYNDLQAFKDACTENTIAIMVEPVQGEGGVHPATKEFMQGL
                     RKFCDENDMLLLIDEVQTGWCRTGAVMSYMNYGIKPDIVSMAKGLGGGMPIGAICATA
                     EVAKAFTPGSHGTTFGGHPVSCAAALAEVNELIDRD"
     gene            complement(1957..2511)
                     /locus_tag="PLN77_04725"
     CDS             complement(1957..2511)
                     /locus_tag="PLN77_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRM43614.1"
                     /translation="MDYLTENVAVNLKRIRQSKGMSLDQVSEQTGVSKSMLSQIEKGT
                     ANPSLGVLGKITSGLRIEFQELIATPLVDSYLVTPENLVPTKELEGQYTVWTCFPYED
                     TRLVEIYRIDIEPGGTYVSGGHGEKTREYLVVTDGVMTVECNGKEHEIHLKQAFRFET
                     DQTHIYRNRGTEKTSCMCFFLDYH"
     gene            complement(2705..3421)
                     /locus_tag="PLN77_04730"
     CDS             complement(2705..3421)
                     /locus_tag="PLN77_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HRM43615.1"
                     /translation="MEHTLALIEKAHEGDKAARDTLAEENMGLVWSMVRRFANRGVEM
                     EDLCQIGSIGLLKAIDKFDPSFEVCFSTYAVPMIAGEIRRFLRSEGMVKVSRPIRELA
                     WKAYAVRERLERENGRDPTLAEIAGELDVTVEEIVEAMESGAEVESLQKTIYQGENHD
                     ISLMDRIPEKENGQEKLLNRIFLEELLGRLPAKERQLIYMRYFQDMTQAQIAGKLGIS
                     QVQVSRMEKKILRQMRMEKV"
     gene            complement(3455..3925)
                     /gene="spoIIAB"
                     /locus_tag="PLN77_04735"
     CDS             complement(3455..3925)
                     /gene="spoIIAB"
                     /locus_tag="PLN77_04735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HRM43616.1"
                     /translation="MRERMKTEEKMRWNTMTLEFESRPCNESFARVSAAAFLAQLNPT
                     VEEVADVKTAISEAVTNAMIHGYRQEKGKIQMKCVLDLEEKVFQVTVKDTGVGIENVE
                     KAMEPMFTTCPELERSGMGFSFMEAFMDELEVRSHPGWGTEVVMKRKIGVHPEM"
     gene            complement(3964..4287)
                     /locus_tag="PLN77_04740"
     CDS             complement(3964..4287)
                     /locus_tag="PLN77_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372950.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HRM43617.1"
                     /translation="MDNRVWVKDAVMTIRLPEEVDHFVADRLREEAGEILVERNVREV
                     VFDFRKTVFMDSSGIGLVMGRFREMELIGGKVRAVHVGERMRRILVMSGVTRVIEVEE
                     EKTWK"
     gene            complement(4495..5910)
                     /locus_tag="PLN77_04745"
     CDS             complement(4495..5910)
                     /locus_tag="PLN77_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM43618.1"
                     /translation="MNCMNCKSVVDVGMEICPNCGFNLRVQRKCFSLSNMYYNLGLDK
                     AEIRDLSGAIDMLRRSLKFNKYNIHARNLLGLVYFETGEAVAALSEWIISKNIMPENN
                     VATEYIATLQAEQAKLDMINQTIKKYNSALKCCRENNEDVAAIQLRKILNQNPKLIKG
                     YHLLALIYIHKGEYEKARKILKKAAKIDKTNSTTLRFLKEVDFQTGTQTSLEPRRFGR
                     RERTEEQREDERERVVSGDTVIIPPTFRETSTAATMLNIGIGLVLGALVVWFLIGPAN
                     TQRINRQADEKVVEYSGKMASQEAQLNQLQSQVDSLNETTASAQQQIQTAQDTAASYE
                     NLLKAVEAQQQGNTSNATNALTEVNPEVLSVDAKAVYDTIYKSMQSSMFQELSQSGVN
                     AFDDANYTEAIDKLSKAKNINDSDYTVLNYLAHAYRLSGDTDNAISVFQEIIDKFPGT
                     QKATKAEQQIEALGGTVKTEE"
     gene            complement(5920..6093)
                     /locus_tag="PLN77_04750"
     CDS             complement(5920..6093)
                     /locus_tag="PLN77_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43619.1"
                     /translation="MLNYEEELKKFKPCLDVDEVEDAIYSRDLTDVIDILKEMMKNDK
                     PEQENETEAEKEE"
     gene            complement(6120..7415)
                     /locus_tag="PLN77_04755"
     CDS             complement(6120..7415)
                     /locus_tag="PLN77_04755"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRM43620.1"
                     /translation="MNYTEAVDYIETIPKFTVKHPPEHTRELLSRLGNPQEGIKIIHV
                     AGTNGKGSVCAYLNAMLLAGGKKTGLFTSPHLVRINERFQINGEDVSDEQFLDAFLKV
                     EKAAKEYEAEGEGHPSYFETLFLMGMLIFKEAGVEYLVMETGLGGRLDATNVVEKPLA
                     CIITSISRDHTEYLGDTLEAIAGEKAGIIKAGVPVIYDASQPGPASVIAAKAKEMGSP
                     AWPMEPSFYEMKTQSREGITFTFAYPGGEKAELAIPYVAKYQMMNASLAFYMMHILQD
                     VHDIPKNVLAEGLSKIKWPCRMEMAAPGVIIDGAHNEDGIAQFVSTAGYFAKENEITI
                     LFTAVADKHYHEMIGEICEGIHPSHVVATQIDGSRVVPAEVLAEDFRKAGCTDVCAEP
                     EIGAAYEKALGKKGSGMLFCVGSLYLAGELKAYLAKRNG"
     gene            complement(7759..9975)
                     /locus_tag="PLN77_04760"
     CDS             complement(7759..9975)
                     /locus_tag="PLN77_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM43621.1"
                     /translation="MKKGKKLHGNKETVVKVCNWLSLPLQALGCCILYLVIEAFSRHS
                     LASAWSYMVESPWVFLYNAFLIFTTFMVVYLFRRRVLVRLIMSCFWLLLGIINGVILA
                     NRVTPFTGPDLRNLSDGAKVVTKYLSKGTMILVAAALVALLAFFVWVWIKGPKFQGKI
                     RWYLNVPLIALVIVLFTGATNLALAKRVLSSYFVNIAFAYQDYGYPYCLAVTLFDTGI
                     SEPNGYSEQLVKQIETSEGEQKEDDTVKPNIIFLQLESFFDPELVNFLNISEDPIPYY
                     RQLMKDYSSGYLRVPVVGAGTANTEFETISGMSLRYFGAGEYPYKSVLSEETCESAPY
                     VLKNLGYATHAIHNNEANFYSRRSVFSRLGFDTFTSEEYMPDISDVTATGWVKDHILT
                     KEIIKTLDATDEPDYIYTISVQGHGDYPTEPVLDDPEITVTGAEDREKNNYSWEYYVN
                     QIHEMDIFVKELTTKLADYPEPVVLVMYGDHLPTMGLKVEDLENRYLYQTEYVIWDNM
                     GLKRKQENIASYQIAAEVMNRVGIHDGTIFRYHQTRRNTKNYQVDLEVLQYDMLYGKR
                     YVYGEDGETPYERVKLQMGALPVTLERIEKSGEDTWYFYGENFTASSRVEVNEEIQDT
                     VYVSPSILMVHSLTLEDGDQITIAQQSNSSTKKVLTRTEPMMYEEPKTVSTPTPMPNP
                     DSTKTDVTQENANTSTAPQDELTAAPSGAPEGEGDKIEPSKTENSDKPAEDGGLQN"
     gene            complement(9976..>11696)
                     /locus_tag="PLN77_04765"
     CDS             complement(9976..>11696)
                     /locus_tag="PLN77_04765"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRM43622.1"
                     /translation="YAGMDRYEARKAMVEDLEKLGLLVKVVDHVHNVGTHDRCKTTVE
                     PMIKPQWFVKMADMAAAASEAEKNGEVNFIPDRFSKIYQNWLNNIHDWCISRQLWWGH
                     RIPAYTCDDCGEITVVRGGMPEKCPKCGSTHLTQDPDTLDTWFSSALWPFSTLGWPEK
                     TPEFDYFYPTDVLVTGYDIIFFWVIRMVFSALEQTKQVPFHHVLIHGLVRDSQGRKMS
                     KSLGNGIDPLEVIDKYGADALRLTLMTGNAPGNDMRFYWERVEASRNFANKVWNASRF
                     IMMNLEKAEVPSEMPKDKLTLADKWILSKVNTLATEVTDNMDRYELGIAVQKVYDFIW
                     EEFCDWYIEMVKPRLYSETDETKGAALWTLKTVLGNALKLLHPFMPFITEEIYCTLNP
                     DEDSIMIAAWPKETEDFAYAEDEAAVEMMKEAVRSIRGVRTSMNVPPSKKASVFVVTE
                     DAAVQETFKNGAVFFGTLAGASEVHVQADKAGIADDAVSAVIPQATIYIPFAELVDLE
                     KEIARLTKEEERLTKEIARSNGMLGNPNFINKAPEAKVQAEKEKLANYQQMMEQVQTR
                     LEQLKK"
BASE COUNT         2463 a         3452 c         2457 g         3314 t
ORIGIN      
        1 ccgcatattt ggagaaggat ccacggacat cgccctcggc tccttttccg gcaatcgtac
       61 cgccctcttc tacacggttc atgatgtctt tcggaattcc gacttcttca ataatctgat
      121 ccaccagctc gcggtcgtag aacttaattc cgagatcctc cgcaattttc ctgccgatgg
      181 catttccttt ggctccgtac tcgcagccga ttgtgataac cagatgtccc ataccgtttt
      241 cctctcacat ttaatttctc tgttttacct gttgtttccg attcacagaa tgaaaagaaa
      301 aacaggacag gggcgaagca tggttctttg ctttttgacg tccttgtcct gttcttttca
      361 cgctatgaga ttttctttgt ttctataaga attctttatt tttccagagc ttttacggtt
      421 tcttcgatga tagcaactgc cttgtcgcag tcctcctcgg ttacgataag cggagggatc
      481 agacgaatga tgtgcgctcc gatggctgtg atcagcagat ggcggtcaag gcagccatgt
      541 ttgacttcta cgccgctgat ggtatcatca aactctacgc cgaccagcag tccctgatgt
      601 ctgacttctt tgacatgcgg cagttttgct aatttttctg cgaagtaatc accgactttc
      661 tttgcatttc cggnnnnnnn nnngtcgcgg tcgatcagtt cgttgacttc tgccagtgct
      721 gctgcacagc tgactggatg gccgccgaag gtagttccgt gagatcccgg ggtaaaggct
      781 tttgctactt cagctgttgc gcagattgcg ccaatcggca ttccgccgcc aagacctttt
      841 gccatggata cgatatccgg tttgatgccg tagttcatgt aagacattac cgcaccggtt
      901 ctgcaccagc cggtctgtac ttcatcaatc agaagcagca tgtcgttctc atcacagaac
      961 ttccggagtc cctgcatgaa ctctttcgtt gccggatgaa caccgccttc gccctgtacc
     1021 ggctcaacca tgatggcaat ggtgttttcg gtacatgcat ctttgaaggc ctgcagatca
     1081 ttatacggtg cgtaggagaa tccgtaggtc atcggtccga atccaacctg gcatccgttt
     1141 cccggctgac cggttgcaga catggcaccg aaggttcttc catggaagcc catctttgcg
     1201 gttacgatgt ggtaacggtt cgggccgtat ttctcgatgc cgtactttct tgccattttg
     1261 atcatggcct cgttcgcctc ggtaccggag ttctgataga agatcttgtc catgccgatg
     1321 gtctcgcaga ctttctcagc aagcagtgcc tgcgggatgg tgtgtggata gttgaaggta
     1381 tgcatgatat cgtcgagctg ctcttttgct gcagcaacaa ctttcgggtt tctgtttccg
     1441 gcgttgttta cggcgattcc tgcgtaaaag tcaagatacg gcgttccgtt ttcgtcgtac
     1501 atgtacatcc cctctgcagt ttctgcaagg aaatcgaaac gctcgtaggt ctcgatcatg
     1561 tacttgttta ctttgtcttt gatgtcctga accgttaatc ctgtgtcttt taatctcata
     1621 gtgttatcct cccatagcgt tgaaaagatt ttttaaataa aaaacaaaaa agcaaagact
     1681 actcgttggt aagcgtcttt gctttgtttt tcgtttatta ttttgtacaa ccatacaata
     1741 gcaccactcc ctatatttgt caattacttt ttttaaaaag ttttttattt ttgttttttc
     1801 tttaacaccc gctttttcta ttcttttttg atgttttttc gtcttccgcc gtattttttt
     1861 catatccgtt gttttttatt ctgatgttcc agcggatttc tgtccattat attgaacagt
     1921 tcattcaaaa aaagaaagcc tgttattctg cggcttttag tgatagtcga gaaagaaaca
     1981 catgcagctc gtcttctccg tccctctgtt gcggtagatg tgggtctggt ctgtctcgaa
     2041 acggaatgcc tgcttcaggt ggatctcatg ctcttttcca ttgcactcca cggtcatcac
     2101 gccgtctgtc accaccaggt actctcttgt tttctctccg tggccgccgc tcacatacgt
     2161 tccgcccggt tcgatgtcga tacggtagat ttccacaagc cgcgtgtcct catacggaaa
     2221 gcacgtccac acagtgtact gcccctccag ctctttcgtc ggaaccagat tctccggtgt
     2281 cacaagataa ctatccacca gcggcgttgc aatcagctcc tgaaattcaa tccgcaggcc
     2341 gctcgtaatc ttcccgagca cgccaagcga cgggttcgcc gttccctttt cgatctgcga
     2401 gagcatactc ttgctgaccc cggtctgctc tgagacctgg tcgagactca tccctttcga
     2461 ctgccggatc cgtttcagat tgaccgccac attttctgtc agataatcca tgttcttcca
     2521 cctgcctttc ttcgtatttc gctcattgta gcacaccttc ctccgatatt ccagtgaata
     2581 tttttaaact ttctgccgcc tttccccgct tcgcctttct tttcttcctt tatccgcccg
     2641 ggtttcaggc acttttctct gcctttccca ggaaaagcgc ccttccgccg gatttgtatt
     2701 tttgtcagac cttttccatc cgcatctgcc gcagaatctt cttttccatc cgcgacacct
     2761 gcacctgcga aatccccagt tttccggcga tctgcgcctg cgtcatgtcc tgaaaatagc
     2821 gcatgtagat gagctgccgc tccttcgcgg gcagtcgtcc caacagctct tccaagaaaa
     2881 tccggttcag cagcttttcc tgcccgttct ctttttccgg aatgcggtcc atcagagaaa
     2941 tatcgtggtt ttccccctga tagatggtct tctgcagcga ttccacctcc gcgccggact
     3001 ccatcgcctc cacgatttct tccacggtaa catcaagctc ccccgctatc tccgccagcg
     3061 tcggatcgcg cccgttctcc cgctccagcc gctcccgcac cgcatacgct ttccacgcca
     3121 gttcccggat cgggcggctc accttcacca tcccctccga ccgcagaaac cgccggatct
     3181 cccccgcaat catcggaacc gcatacgtcg aaaaacagac ttcaaacgaa ggatcaaatt
     3241 tatcaatcgc cttcaacagc ccaatgctcc cgatctggca cagatcctcc atctccaccc
     3301 cgcggtttgc aaaccgcctc accatgctcc agaccagtcc catattttcc tctgccagtg
     3361 tatcccttgc cgctttatcc ccttcgtgtg ccttttcgat cagggcaagc gtatgttcca
     3421 tgcattctga ctcctatgac ttccgatccg gcccttacat ttccggatgc acaccgattt
     3481 tccgtttcat caccacttcc gtcccccatc ccggatgcga ccttacttcc agctcatcca
     3541 taaacgcctc cataaacgaa aaccccatcc ccgaccgctc cagctccggg caggtcgtaa
     3601 acatcggctc catcgccttc tccacatttt caatcccgac gccggtatct tttaccgtca
     3661 cctgaaacac cttctcctcc agatccagca cacacttcat ctgaattttc cctttttcct
     3721 gccggtatcc gtgaatcatc gcattcgtaa ccgcctccga aatcgccgtc ttgacatccg
     3781 ccacttcctc caccgtcgga ttcagctgcg ccagaaacgc cgccgcagac acccgcgcaa
     3841 aactctcatt gcagggacgg ctctcaaatt ccagcgtcat cgtattccac cgcatcttct
     3901 cttctgtttt catcctttct cgcatcatcc cattcccctt tctgcccact ccccgtccaa
     3961 aacctacttc cacgtcttct cctcttccac ctcaatcacc cgcgtcaccc ccgacatcac
     4021 cagaatcctc cgcatccgct ctcccacatg caccgctctc accttcccgc caatcagctc
     4081 catctcccga aacctcccca tcaccagtcc aatccccgaa ctgtccataa acaccgtctt
     4141 ccgaaaatca aacaccacct ccctcacatt cctctccacc aaaatctccc ccgcctcctc
     4201 ccgaagccgg tcagccacaa aatgatccac ctcttccgga agccgaatcg tcatcaccgc
     4261 atccttcacc caaacacgat tatccatttc ctatcctccc tctttcctgt cataaaataa
     4321 aaaacacaaa agatttttcg ccgtaaagcg aatttcatct tttgtgtttt atcttctcac
     4381 ttttatactt ttttgccctt cgtcccacgc accctccgcg tagtcagtgg tatcagcccg
     4441 acgaaacagc gccgcatctc ctgcggcatt ctgccccgtg ggcgtccgtc ctcactattc
     4501 ctccgtcttc acagtccccc ccagcgcctc aatctgctgc tcggccttag tagccttctg
     4561 ggttcccggg aacttatcga tgatctcctg gaacaccgaa atcgcattat cggtatcccc
     4621 actcagccga tacgcatgcg ccagatagtt aagcaccgta tagtcactgt cgttgatatt
     4681 cttagccttc gaaagcttat caatcgcctc agtgtaattc gcatcgtcaa atgcattcac
     4741 acccgactgc gaaagctcct ggaacataga cgactgcatg ctcttgtaaa tagtgtcata
     4801 cacagccttt gcgtccacag aaagcacctc cgggttgacc tcggtcagtg cgtttgttgc
     4861 gttgctcgtg ttgccctgct gctgcgcttc cacagccttt aaaaggttct cgtaggaagc
     4921 cgccgtatcc tgtgccgtct ggatctgctg ctgcgcagaa gccgtcgttt cgttcagaga
     4981 atccacctgg ctctggagct ggttcagctg ggcctcctgg ctcgccatct tgccgctgta
     5041 ttccacaacc ttctcgtccg cctgccggtt aattcgctgc gtattcgcag gcccgatcag
     5101 gaaccacaca accagtgcgc caagcaccag cccaataccg atattcagca tcgttgccgc
     5161 tgtagacgtc tcgcggaacg tcggcggaat aatcaccgta tccccgctta ccacacgctc
     5221 acgctcgtcc tcgcgctgct cctccgtgcg ctccctgcgg ccgaaccgcc gcggctccag
     5281 cgacgtctgc gtacccgtct ggaaatcaac ctccttcagg aaacgaagcg tcgtcgagtt
     5341 cgtcttgtca attttcgccg cctttttcag aatcttccgc gctttctcat actcaccctt
     5401 gtgaatataa atcagcgcca gcagatgata tcccttgatc agcttcggat tctggttcag
     5461 gatcttgcgc agctggattg cagccacatc ctcattgttc tcgcggcagc atttcagcgc
     5521 actgttgtat ttcttgatcg tctgattgat catatccagc ttcgcctgct ccgcctgcag
     5581 cgtcgcaata tactccgtcg ccacattatt ctccggcata atattcttgc tgatgatcca
     5641 ctcacttaac gccgcaaccg cttcacccgt ctcaaaatag acaaggccta acaagttccg
     5701 cgcatggata ttgtatttgt taaacttcag gctccgccgc agcatatcga tcgctccgga
     5761 aagatcccgg atctctgcct tgtcgaggcc cagattgtaa tacatattcg aaagagaaaa
     5821 acattttctc tgtactctca ggttaaaacc acagttggga cagatctcca ttccgacgtc
     5881 cacaaccgat ttacaattca tacagttcat tccggcacct tactcttcct tttccgcttc
     5941 tgtctcgttt tcctgttccg gcttgtcgtt tttcatcatt tctttcaaaa tgtcgatcac
     6001 atccgtcaga tcacggctgt aaatcgcatc ctccacctcg tccacatcga gacacggttt
     6061 aaacttcttc agttcttctt cgtaatttaa cataaacaaa aaattccttc ctgtattcgc
     6121 taaccattcc gctttgcaag atatgctttc agttcgcccg ccagatacag agaacccacg
     6181 cagaacagca tgccgctgcc ctttttgccc agtgccttct catatgctgc cccgatctcc
     6241 ggttccgcgc agacatccgt gcatcctgct tttctgaagt cctctgcaag cacttccgcc
     6301 ggtacgacgc ggcttccgtc gatctgcgtt gcaacaacat gggacggatg gatgccctcg
     6361 cagatttcac cgatcatctc atgatagtgc ttgtctgcaa ctgccgtgaa cagaatcgtg
     6421 atctcgtttt ctttcgcaaa atagcctgcc gtgctgacaa actgtgcaat gccgtcctcg
     6481 ttgtgcgcgc cgtcgatgat gacgcccggc gcagccattt ccatacggca cggccatttg
     6541 atcttcgaaa ggccttccgc aagcacgttc ttaggaatgt cgtgcacgtc ctgcagaata
     6601 tgcatcatgt agaacgcaag gcttgcattc atcatctggt attttgccac gtacggaatc
     6661 gcaagctctg ctttttcgcc gcccgggtat gcaaaggtaa aggtgattcc ctcgcggctc
     6721 tgtgttttca tctcatagaa cgacggctcc atcggccatg ccgggcttcc catctctttc
     6781 gcctttgcgg caatgacaga agccggacct ggctggctgg cgtcgtagat caccgggaca
     6841 ccggctttga tgatgccggc tttctctccc gcaatcgcct cgagggtgtc gccgagatac
     6901 tcggtgtggt cgcggctgat ggacgtgatg atgcatgcca gcggcttttc gaccacattt
     6961 gtcgcatcca gccgtccgcc aaggccggtc tccatgacca ggtattccac gccggcttct
     7021 ttgaaaatca gcattcccat caggaacagc gtctcaaaat agctcggatg gccttcgccc
     7081 tccgcctcgt attctttggc tgctttttcc actttgagaa atgcgtcgag aaactgctca
     7141 tccgacacat cttccccgtt gatctggaac cgctcgttga tgcgtacaag gtgcggggag
     7201 gtaaaaagtc ctgtcttttt gccgcctgca agcagcatcg cattcaagta cgcgcagacg
     7261 cttccctttc cgttcgtgcc cgccacatgg ataattttga tcccttcctg cggattccca
     7321 aggcgcgaaa gaagctccct cgtatgttcc ggcggatgct tcaccgtaaa tttcggtatt
     7381 gtttcgatat aatcaactgc ttctgtataa ttcaccttat ttcaccattc ttcagtattt
     7441 tagggctgct ttcagaccct agtacatcgt ttcgtaaata actataccaa tcacgcagga
     7501 tgcagattcg agtgtgcagg tataaaaatg taggcgtagc gggctacgcc gaggctttta
     7561 tgcctgtgca atcggatttg cagacaagtg agtggtatag ttatttcgaa aacgatgtac
     7621 tagtatcatt ataatcgagt gaccggatat gtccatagca attttcaaaa ttttgtcccg
     7681 acttttcgac tttccccgta aaaagataca gtgggacgtc aaaatccgcc ccactgtatg
     7741 atctttcgga cactcttttt aattctgcag accgccgtct tcggccggtt tatcgctgtt
     7801 ttctgttttt gacggctcaa tcttgtcgcc ttcgccctcc ggcgcaccgg acggcgctgc
     7861 cgtcagttca tcctgcggcg cagtgctcgt attggcattt tcctgcgtca catctgtttt
     7921 cgtgctgtcc gggttcggca tcggcgtcgg tgtagatacc gtttttggct cttcatacat
     7981 catcggctct gtccttgtca gcaccttctt cgtcgagctg ttgctctgct gcgcgattgt
     8041 aatctgatca ccgtcttcca gggtcagact atgcaccatc agaatcgacg ggctgacata
     8101 aacggtatcc tgtatctcct cgttgacttc cacacggctc gatgccgtaa agttttctcc
     8161 gtaaaaatac caggtatctt ctccggattt ttcgattcgt tccagtgtta ccggcagagc
     8221 acccatctgc agcttcacac gctcatacgg tgtctctccg tcttctccgt aaacgtaccg
     8281 ttttccatac agcatatcat actgcagcac ctcgagatcc acctgataat tcttcgtatt
     8341 ccgtctcgtc tgatgatacc ggaaaatggt tccgtcatga attcccacac ggttcataac
     8401 ctctgctgca atctggtagg aagcaatgtt ttcctgtttc cttttcagtc ccatgttatc
     8461 ccagatcacg tattcggtct gatacagata gcggttctcc agatcttcta ctttcagtcc
     8521 catcgttggc agatggtccc cgtacatcac aagtaccacc ggctccggat aatccgcaag
     8581 ctttgtcgtc agctctttga caaatatgtc catctcatga atctgattta cataatattc
     8641 ccaggagtaa ttattttttt cccggtcttc cgcgcctgtc actgtaatct ccggatcgtc
     8701 aagcaccggc tctgtcggat aatcaccgtg gccctgtacc gaaatcgtat agatataatc
     8761 cggttcgtct gtcgcatcga gcgtttttat gatctctttt gtcagaatat ggtcttttac
     8821 ccatcctgtc gccgtcacat ccgagatatc cggcatatat tcctccgagg taaaggtatc
     8881 aaaaccaaga cgcgagaaca cacttcttct ggaatagaag tttgcttcat tgttgtggat
     8941 cgcatgggtc gcatagccaa gattctttaa aacatacggc gcactctcac acgtctcctc
     9001 tgaaagcacc gatttgtacg gatactcacc cgctccgaaa taccggaggc tcatgccgga
     9061 aatagtttca aactctgtgt ttgctgttcc ggctcccacc accggcacac ggagataacc
     9121 ggaagaataa tccttcatca gctgccggta atacgggatc gggtcctcgg aaatgttgag
     9181 gaaattaacc agctccggat cgaaaaagga ctccagctgc aaaaagataa tattcggttt
     9241 taccgtatca tcctcttttt gttcgccttc actcgtctca atctgtttca cgagctgttc
     9301 ggaatagccg ttcggctctg aaattcccgt atcaaacagg gtgactgcca gacagtacgg
     9361 atagccgtaa tcctggtagg caaatgcgat gttgacgaaa tagctggaaa gcacacgctt
     9421 tgcaagcgcc agattcgttg ctcccgtaaa gagtacaatt accagagcaa tcaacggcac
     9481 attcagatac cagcggattt tcccctgaaa tttcggtcct ttgatccaga cccatacaaa
     9541 aaatgccaat aatgccacaa gagctgccgc aacgaggatc atggtacctt tcgacagata
     9601 cttcgtcacg accttggcac catcgcttaa gttccgcaaa tccggcccgg taaacggtgt
     9661 gacgcggttc gccagaatca caccgttgat gataccgagc agcagccaga aacagctcat
     9721 gatgagccgc accagcacac gtctgcggaa cagataaact accataaaag tagtgaagat
     9781 caaaaatgca ttgtacaaaa agacccatgg gctttccacc atgtagctcc atgcagatgc
     9841 cagcgaatgg cgggaaaatg cttcaatcac cagatacaga atacagcatc ccagggcctg
     9901 cagcggcaaa gagagccagt tgcagacttt cactacggtt tccttgtttc catgcagttt
     9961 ttttcctttt ttcatttatt ttttcagctg ctccaatctt gtctgaacct gctccatcat
    10021 ctgctgatag ttcgccagtt tttctttctc tgcctgaact tttgcttccg gagctttgtt
    10081 gataaagttc gggttgccaa gcatgccgtt ggaacgtgcg atctcttttg tcagacgctc
    10141 ttcctctttc gtaaggcggg cgatctcttt ttccaggtca accagctcgg caaacggaat
    10201 gtagatcgtt gcctgcggga tcactgcgga taccgcatcg tctgcgatgc cggctttgtc
    10261 tgcctgtacg tgaacttcgc ttgctcctgc cagagttccg aagaatacag caccgttttt
    10321 gaaggtttcc tgtacagcag catcttccgt tacaacgaat acgcttgctt tctttgacgg
    10381 cggtacattc atgctggtgc ggacaccacg gatgcttctt accgcctctt tcatcatctc
    10441 aacggctgcc tcatcctctg cgtatgcaaa gtcttcggtc tctttcggcc atgcggcgat
    10501 catgatggaa tcctcgtccg gattcagtgt gcagtagatt tcctctgtga tgaacggcat
    10561 aaacggatgc agcagcttta atgcattgcc aagaacggtt tttaaggtcc acagtgcagc
    10621 gcccttcgtc tcgtcggtct cgctgtacag acgcggtttt accatctcga tgtaccagtc
    10681 gcagaactcc tcccagataa agtcgtaaac cttctgtacg gcgataccga gctcgtagcg
    10741 gtccatgttg tccgttactt ccgttgccag cgtgtttacc ttagaaagaa tccatttatc
    10801 tgccagtgtc agtttgtctt ttggcatttc agacggaact tctgcttttt ccaggttcat
    10861 catgatgaac ctggaagcgt tccatacttt gtttgcgaag ttacggctgg cttctacacg
    10921 ctcccagtag aaacgcatat cgtttccagg tgcattaccg gtcatcaaag tcagacggag
    10981 agcatctgca ccgtatttgt caatgacttc gagcggatcg ataccgtttc cgagggattt
    11041 actcatcttg cgtccctggg aatcacgaac gagtccgtgg atcagtacgt ggtggaacgg
    11101 aacctgtttg gtctgctcca gtgcggagaa taccatacgg ataacccaga agaagatgat
    11161 gtcgtatccg gttacgagta cgtcggtcgg atagaagtaa tcaaattccg gtgttttctc
    11221 cggccatcct aacgtggaga acggccacag agcagaactg aaccaggtgt ccagggtatc
    11281 cgggtcctgg gtgagatggg tgctgccgca tttcgggcat ttttccggca ttccgcctct
    11341 tacaaccgtg atctcgccac agtcatcaca ggtataggcc gggatacgat gaccccacca
    11401 gagctgacgg gaaatacacc agtcgtggat attgttcagc cagttctgat aaatcttgct
    11461 gaaacgatcc gggatgaagt ttacttcccc gttcttctct gcttcgctgg cagcggcagc
    11521 catatccgcc atttttacaa accactgtgg tttgatcatc ggctctacgg ttgtcttaca
    11581 tctgtcgtgg gttcctacgt tgtgaacgtg atccactact tttacaagaa ggccaagctt
    11641 ctcgagatcc tctaccatgg cttttcttgc ctcgtagcgg tccatgccgg cgtatt
//
LOCUS       DAOMUF010000055        11602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1322_length_11602_cov_3.362085, whole genome shotgun sequence.
ACCESSION   DAOMUF010000055 DAOMUF010000000
VERSION     DAOMUF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1322_length_11602_cov_3.362085"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..2532
                     /locus_tag="PLN77_04770"
     CDS             157..2532
                     /locus_tag="PLN77_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRM43623.1"
                     /translation="MNQKALKTLEYDKIIEQLASYASSPLGRKKCLELVPSSSLSQIT
                     RMQHETHDALTRLFKKGHISFGDAKDIRGALKRLEIGSSMNITELLQTAALLENTARD
                     KAYGRRENADQTPDSLEDLFETLEPLTLLSTEIRRCILSEDEISDDASPNLRRIRRSI
                     KSCEEKIHSQLISLVNGSYRTYLQDAVITMRDGRYCIPIKSEYKGQVPGMVHDQSSTG
                     STFFVEPMAVVKLNNDIRELEIEEAKEIEVILSTLSAQADLHRDEIRYDLENMVELDF
                     IFARAALAMEQNANEPIFNTEGKIHLRKARHPLINAKKVVPIDLSLGEDFDLLIVTGP
                     NTGGKTVSLKTVGLLTLMGQSGLHIPALDRSSLSVFDEVYADIGDEQSIEQSLSTFSS
                     HMTNVVSFLDKADRNSLVLFDELGAGTDPTEGAALAIAILSTLHERGIRTMATTHYSE
                     LKIYALSTPGVENACCEFDVETLSPTYRLLVGVPGKSNAFAISSKLGLPDYIINKAKD
                     EISEQDESFEDVLTTLEQNRITLEREKEELARTRAEVESLKQSLAKNQEQIDARKEKM
                     ISNATEEAQRILREAKEYADQTMRDFQKFGKENVSVREMEQKRTALREKMNKLDKKKA
                     KEVPKRTGNLRPQDLHLGDAVKVLSMNLKGTVSTRPDSKGNLFVQMGIMRTKVHISDL
                     VLIDEAVITGPSISRTSSGKSKLSKAATISTEINLLGKTVDEAVAELDKYLDDAYLSH
                     LPSVRVVHGKGTGALRKGIHNYLRRQKHVADFHLAEFGEGDAGVTIVTFKK"
     gene            2607..3311
                     /locus_tag="PLN77_04775"
     CDS             2607..3311
                     /locus_tag="PLN77_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM43624.1"
                     /translation="MASKQKILIVDDDNNIAELISLYLTKECYDTKIVNDGEEAVREF
                     HSFQPNLILLDLMLPGMDGYQVCREIRHDSDVPIIMLSAKGEVFDKVLGLELGADDYI
                     IKPFDSKELVARVKAVLRRFQPSRTIPVSATAGKCVEYPDLIINQTNYSVIYQGRTVD
                     MPPKELELLYFLASSPNQVFTREQLLDHIWGYEYIGDTRTVDVHIKRLREKIKDHASW
                     AITTVWGIGYKFEVKK"
     gene            3311..4729
                     /locus_tag="PLN77_04780"
     CDS             3311..4729
                     /locus_tag="PLN77_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM43625.1"
                     /translation="MRRTLYRKLILIYVILAIAGFTLISTVGSRMVEDSLVSSASTSL
                     YREATQIASKQGDLYYHSERSLTDLYNSLSALASYHNSQIWLISADGEILLNTAAALD
                     EENPEIIDGFDPVALGSEYYSTGTFFGYFEDKMLSVMVPVTSDFRINGYVAIHLPLQD
                     VYQQRESLLARIDFLFLLVFGLSFVILIFFSLVVYRPLKKIINGAKEYASGNLTHKID
                     VKSHDEMGYLASTLNYMSGELNKTGEYQRKFVANVSHDFRSPLTSIKGYVEAILDGTI
                     PPEMQEKYLKIVLFETERLHKLTQSLLTLNDIDDKGNMLDLTSFDINAVIKDTAASFE
                     GTCTKKRISISLILASRTLYVCADMGKIQQVLYNLIDNAIKFSNNDSTITVETTEKHG
                     KVFVSVKDTGCGIPKASITKIWERFYKLDASRGKDRKGTGLGLSIVKEIITAHNQNIN
                     VISTEGVGTEFIFTLAKGEAPK"
     gene            4793..5284
                     /locus_tag="PLN77_04785"
     CDS             4793..5284
                     /locus_tag="PLN77_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRM43626.1"
                     /translation="MNPNTIDSIIKQLIASHTTISTMESCTSGLIASLITDTEGASAI
                     FPGGYVTYLNETKVLVGVDPSVIEIHGVYSPECAEAMARTVQENLHTAIAIGITGTTG
                     NLDPNNADSVQGVVYFCILYKTQAHTFTFQTNVSGMTRHEIKQLYAEQVFAELEMILT
                     SSL"
     gene            complement(5327..5827)
                     /gene="trmL"
                     /locus_tag="PLN77_04790"
     CDS             complement(5327..5827)
                     /gene="trmL"
                     /locus_tag="PLN77_04790"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HRM43627.1"
                     /translation="MAHLNIVLHEPEIPANTGNIGRTCVATGTRLHLIKPLGFDISDK
                     AVRRAGLDYWPKLDVTVYESYEDFLERNPGAKIYYATTKGPQTYVDVQYEEDCFIMFG
                     KESAGIPEDILYNNQETAVRIPMINDIRSLNLSNSVAIVLYEALRQNNFDHMQLAGHL
                     RTHEWR"
     gene            complement(5860..6840)
                     /locus_tag="PLN77_04795"
     CDS             complement(5860..6840)
                     /locus_tag="PLN77_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRM43628.1"
                     /translation="MRKGKLQQSVLVRSVFKMLGQKREDAAGPAVGRGVSALAGEKET
                     QVFSCAPVLVKDPEDAVLAVCRVCNSVAAAGASAKGVLVSLMLPEAMEEKDLKELMRA
                     VDGACVRYGTAVIGGHTEVTDGVLYPVLTVTAAGGTTRAGLFDPSCVTADLDLVVTKG
                     IALGAVSYLAKTRRDELLTRFSPGFIHGAADFSEDYSVCREAEIAIEHGVAAMQDLSE
                     GGIFGALWEMADGAGIGLDVALKRIPIQQETVEICEFFGVNPYQMLSTGALLIAAADG
                     EGLVQKMALEGIPSAVIGRTTSGKERILRNGEEVRYLDKPQMDEMYRVGR"
     gene            complement(6976..8529)
                     /locus_tag="PLN77_04800"
     CDS             complement(6976..8529)
                     /locus_tag="PLN77_04800"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRM43629.1"
                     /translation="MKNSRKMRGLLVMLIAFAFCFLAEPVKADDRTALDGIYVEDISV
                     GGMTEEQITQAVNDKIEQLKPSVINLSAGEQNAQVTAGDLGLSCANPEVAREAVTIGQ
                     EGNVLKRFLTQNRLKNGETVTFSLKYTVDGEAARQAVENNTAVLNREATDATLTRENG
                     EFIVNPGQTGCSVNVDESTAKVVNYLTTSWRGGIGGVELVTEETPAGGNPEQLALVKD
                     LLGEGMTEYGNGTSGRKQNVAVGAEKINGTLVQPGEEFSVEAVVVPFDAENGYALAAS
                     YEMGKVVDSYGGGICQVSTTLYVAVLKAELEVTERYSHSMIVHYVDPSMDAAIAEGLK
                     DLKFINNTDAPIYIEASADGSTLHFAIYGHETRDPNRTVRYESETTSTEDPTPSLTED
                     ANASFGTIEQTSYGYQGSTARLWKIVNDNGNETKEQVNSSTYRMTSDVYTVGTKTDNA
                     AARAEMQAAIAANDLAKAKEVAAKYSQSSSSSSSSEKKEDSSDDSDSDGTEKTDDSSD
                     TEKTEDDED"
     gene            complement(8585..8743)
                     /locus_tag="PLN77_04805"
     CDS             complement(8585..8743)
                     /locus_tag="PLN77_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43630.1"
                     /translation="MSNKVNRKAPTEAQKKKKDPLAKKRNAAKMLALILAVVMVLGMV
                     LPALLWLV"
     gene            complement(8768..9505)
                     /locus_tag="PLN77_04810"
     CDS             complement(8768..9505)
                     /locus_tag="PLN77_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM43631.1"
                     /translation="MKIQDFVVLDLETTGLSVKEDQILEIGAVKVQGGEVTASYETFV
                     NPGRKVPERITELTGIRDEMIANAPDVETAVRGFLDFCGGLPLLGHNILFDYSFIKQA
                     AINARLDFEKEAWDTLKIARKALPDLESRSLEALCGYYQIPREHAHRAMDDVLETLAL
                     FRKLEEGFSEDHPEWFAAAPLKAKMKREVPATEAQKKYLADLIRYHELDLEPEWGALT
                     KSRASRMIDQIILAHGRMEKRQRTEKK"
     gene            complement(9547..11373)
                     /locus_tag="PLN77_04815"
     CDS             complement(9547..11373)
                     /locus_tag="PLN77_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HRM43632.1"
                     /translation="MKIILTAVNAKYIHSNLAVYTLQASAEKAGVFPEIREFTINQSK
                     DSMLRSLFLAHADVVCVSCYIWNISIVEDLITEYHKISPETKIWLGGPEVSYHAEEML
                     EQYPFLDGIMKGEGEITFRELAVYYQNQENGTEGKTLEEIHGITYRDAEGAIKSNPWR
                     PVMDLSEVDFLYANLKKFENRIIYYESSRGCPFSCSYCLSSIDKRLRFRNLDLVKKEL
                     AFFLEQKVPQVKFVDRTFNCKKDHAMAIWKFIAEHDNGVTNFHFEIAADLITEEELKL
                     LNTLRPGLVQLEIGVQSTNPQTIEAIHRKMDFGRVTEIVNRIAKGRNIHQHLDLIAGL
                     VKDLGVGLLIISHDLSVLADVCAGLPYEDYDSFRRSFADVYALRPQQLQLGFLKVLRG
                     SFMYEHTEEYDCHYQEREPYEVLYTKWLPYDDVLKLKDVEEMVEVYYNSGQFVHTLPM
                     IERLYENPFDFFQELGDFYRAKGYSEAAHNRIQRYEILLEFLQDEKQQDEAFFRQMMV
                     LDLYARENMKTRPRFAKDPSEWKNESRDFYQKEAETRTLLPSYTTYDWKQLQRMTHVE
                     VFDYDVLGNGEKARTVLLFDYQKRDPLTGNAEMIDCSELFYA"
BASE COUNT         2739 a         3487 c         2571 g         2805 t
ORIGIN      
        1 tccttttgcg aagcaaaact ttaaatgagg gctgcgaatg ttacagttcg cagggtacct
       61 gtgaactgta acatcagatc cgtgcggtat tgcatcaaaa ataccgcgtt atctgtagac
      121 aatgactgaa ttatgaatta cgaaaaggta agtattatga atcagaaagc attaaaaacg
      181 ctcgaatacg acaaaattat cgaacagctg gcttcgtatg ccagttctcc gctgggccgg
      241 aagaaatgtc tggagctcgt tccttcttct tccctctccc agattacgcg gatgcagcac
      301 gagacacacg acgcgctcac ccggctcttt aaaaaaggcc acatttcctt tggtgatgcc
      361 aaagatatcc gcggcgcatt aaagcgtctc gaaatcggca gctccatgaa catcacggag
      421 cttctgcaga ccgccgcact tctggaaaac acggcacgag acaaagcgta cggccgccgc
      481 gaaaatgcgg accagacgcc ggacagcctc gaagatctct tcgaaacact ggagcctctg
      541 acgctgcttt ccacggaaat ccgccgctgt attctctccg aggatgaaat cagcgatgat
      601 gcaagcccga atctacgccg catccgtcgt tccatcaaat cgtgtgagga aaaaatccac
      661 agccagttaa tctcgctcgt caacggttcc taccgcacgt atctgcagga tgccgtcatc
      721 acgatgcgcg acggccgcta ctgtatcccg atcaaatccg agtacaaagg ccaggttcct
      781 ggcatggtac atgaccagtc ctcgaccggt tccaccttct ttgtcgagcc gatggccgtt
      841 gtcaaactga acaacgatat ccgtgagctg gaaattgaag aagcaaaaga aatcgaggtc
      901 atcctctcta cgttgagtgc ccaggcagac ctgcaccgcg atgagatccg ttacgacctc
      961 gaaaacatgg tagagcttga cttcatcttc gcgcgcgccg ctcttgcaat ggagcagaac
     1021 gccaatgagc cgatcttcaa caccgaaggt aaaatccacc tgcgcaaggc acgccacccg
     1081 ctgatcaatg cgaaaaaagt cgttccgatt gatctgtcgc tcggcgaaga tttcgacctt
     1141 ctgatcgtca ccggaccgaa taccggcggt aaaacggttt ccttaaagac ggtcggactc
     1201 ttaacactga tgggccagtc cggacttcac attccggcac tcgaccgctc ctccctttcc
     1261 gttttcgacg aggtttacgc cgatatcggt gatgagcaga gcatcgagca gtcgttgagt
     1321 accttctcct cccacatgac gaatgtagtt tccttcctcg acaaggcaga ccgcaattcc
     1381 ctcgtcctgt tcgatgagct cggcgccggt accgacccga cagagggcgc ggcgcttgcc
     1441 atcgcgatcc tctctacgct ccacgagcgc ggcatccgca ccatggcaac gacacactac
     1501 agtgaactga aaatctatgc cttatccaca ccgggcgtgg agaatgcctg ctgtgaattc
     1561 gatgtcgaga ctttaagccc gacataccgc ctgctcgtcg gcgttcctgg aaagagtaat
     1621 gcgtttgcga tttccagcaa actcggcctg ccggactaca tcatcaacaa ggcaaaagac
     1681 gaaatcagcg agcaggatga atcgtttgag gacgttctga ccactctcga gcagaaccgg
     1741 atcacgctgg aacgcgaaaa agaagagctt gcccgcaccc gcgcggaagt cgaatccctg
     1801 aagcagagcc ttgccaagaa tcaggaacag atcgacgcaa gaaaagaaaa gatgatctcc
     1861 aatgcaaccg aggaggcaca gagaattctc cgcgaggcga aggaatacgc cgaccagacg
     1921 atgcgcgatt tccagaaatt tggaaaagag aatgtttccg tccgcgaaat ggagcagaaa
     1981 cgtaccgctc tgcgtgaaaa gatgaacaag ctcgataaga aaaaggcgaa ggaagtgccg
     2041 aagcgcaccg gaaatctccg cccgcaggat cttcatctcg gcgatgccgt caaagtctta
     2101 agcatgaatc tcaaaggtac tgtcagcacc cgcccggatt cgaagggaaa tctcttcgtc
     2161 cagatgggta tcatgcggac caaagtgcat atctccgacc ttgtgctgat cgacgaggct
     2221 gtcatcaccg gcccgtccat ctcccgcaca agcagcggaa aatcgaagct ttccaaggct
     2281 gccacgattt ctaccgagat caatctgctc ggaaaaacgg tggatgaagc ggttgccgaa
     2341 ctcgacaaat atctcgatga cgcttatctc tcccatctgc cgagtgtccg cgttgtccac
     2401 ggaaaaggaa cgggcgcact gcggaaaggc attcacaatt atctgcgccg ccagaaacac
     2461 gtagcggact tccatttagc ggaattcggc gagggcgatg ccggcgttac gattgttacg
     2521 tttaagaaat aaaaactcag gaaaatatgg gacggaacgc atactgtatg cggttccgtc
     2581 ctgtccccag tgaaggagaa tcttttatgg cttccaaaca gaaaattctg atcgtcgatg
     2641 acgataacaa tatcgcagaa ctgatttctc tctatctgac caaggaatgc tatgacacaa
     2701 aaattgtaaa cgatggagaa gaggctgtcc gggagtttca ctccttccag ccgaacctga
     2761 ttctgctcga cctgatgctg cccggcatgg acggctacca ggtctgccgc gaaatccggc
     2821 acgattccga tgttccgatc atcatgctct cggcaaaggg cgaggtgttc gacaaagtcc
     2881 tcggcctgga actcggcgcg gatgactaca tcatcaaacc atttgattcc aaggagctcg
     2941 ttgcccgtgt gaaagcagtg ctgcggagat ttcagccatc acgcacgatc ccggtgtccg
     3001 cgaccgcagg aaaatgtgtc gagtacccgg atcttatcat caaccagacg aactattccg
     3061 tcatttacca gggccgcacc gtcgatatgc cgccgaagga actggagctt ctctacttcc
     3121 ttgcttcctc accaaaccag gtctttacgc gagaacagct cctcgaccat atctggggct
     3181 atgagtacat cggcgatacc cgcacggtag acgttcatat caaacgtctc cgcgaaaaaa
     3241 tcaaggacca tgcctcctgg gccatcacga cggtctgggg catcggttac aagtttgagg
     3301 ttaaaaaata atgcgacgca cgctttaccg gaaattaatt ctcatttatg tcattctcgc
     3361 cattgccggc tttacgctga tctcaacggt cggctcccgc atggtggagg acagtctggt
     3421 ctccagcgcg agcaccagtc tgtaccggga ggccacgcag atcgcttcca aacagggcga
     3481 cctttattac cacagtgagc gttccctgac ggatctttac aattcgctca gtgcactggc
     3541 ttcctatcac aattcccaga tctggctcat cagcgcagac ggggaaattc tgctgaacac
     3601 ggcagctgca ctcgatgagg aaaacccgga gatcatcgac ggttttgatc cggttgcgct
     3661 cggcagcgag tattacagca cgggaacgtt tttcggctat ttcgaagaca agatgctgag
     3721 cgtcatggtt cccgtcacct ccgatttccg gatcaatggc tatgtcgcca tccaccttcc
     3781 gctgcaggac gtctatcagc agagggaaag tctgctggca cggattgatt ttctgttcct
     3841 gctcgtgttc ggactctcgt ttgtgattct gatctttttc tccctcgtcg tgtaccggcc
     3901 gctgaaaaag atcatcaacg gtgcaaagga gtatgcctcc ggcaacctga cccacaaaat
     3961 tgatgttaaa tctcacgatg agatggggta tctcgcttcc acgctgaact acatgtccgg
     4021 cgagctgaac aagaccggcg aataccagcg gaaatttgtg gcgaacgtct ctcacgactt
     4081 ccgctcaccg ctgacctcga tcaaaggcta tgtggaggca atcctcgacg gaacgattcc
     4141 gccggaaatg caggaaaaat atctgaaaat cgttctgttt gagacagaac ggctgcacaa
     4201 gctgacgcag agcctgctga cgctgaacga catcgacgat aaagggaata tgctcgatct
     4261 gacctcgttt gacatcaacg ccgtcatcaa ggataccgcc gcttcgtttg agggaacctg
     4321 cacgaaaaag cggatttcca tttcactgat cctcgcatcg cgcaccttat acgtctgcgc
     4381 cgatatggga aaaatccagc aggtgctgta caatctcatt gataatgcaa tcaaattcag
     4441 caacaacgat tccaccatca cagttgagac aacggagaag cacggcaaag tcttcgtctc
     4501 cgtcaaggac accggctgcg gcattccaaa ggcaagcatc acgaaaatct gggagcgctt
     4561 ctacaagctc gacgcctccc gcggcaagga ccgcaaagga acgggacttg ggctttcgat
     4621 cgtcaaagag atcatcactg cgcataacca gaatatcaat gtcatcagta cagagggcgt
     4681 tggtaccgaa tttatcttta cactcgccaa aggcgaagca ccaaaataag cccctccgcc
     4741 gaggggcttt tttgccagac atatcaaact aactctggag gtaattttca ctatgaaccc
     4801 aaacaccatt gattccatca tcaaacagct cattgcatcc cacaccacca tttccaccat
     4861 ggaaagctgc acttccggcc tgatcgcctc cctgatcacc gacaccgaag gcgcctccgc
     4921 cattttcccc ggcggctacg tcacgtatct gaacgaaacc aaagtcctcg tcggcgtcga
     4981 cccttccgtc atcgaaatcc acggcgtcta ctcccccgaa tgcgccgaag ccatggcccg
     5041 cacagtacaa gaaaacctgc acaccgccat cgccatcgga atcaccggca caaccggaaa
     5101 tctcgacccc aacaacgctg acagcgtaca gggcgtcgtg tacttctgta ttttatacaa
     5161 aacccaagcc cacaccttca ccttccagac caacgtctcc ggcatgaccc gccacgaaat
     5221 caaacagctt tacgccgagc aggtcttcgc cgaattggaa atgatcttaa cttcttcctt
     5281 ataaaaagaa tgcagctccg tctacgatca ccagagtccc gtccgtctac ctccactcat
     5341 gcgtccgcaa atgccccgcg agctgcatat ggtcaaagtt attctgccgc agtgcctcgt
     5401 acagtacgat cgccacggaa tttgaaaggt tcagcgaccg aatatcatta atcatcggaa
     5461 tccgcaccgc cgtctcctga ttattgtaca gaatatcctc tggaatcccc gcgctttcct
     5521 ttccgaacat gataaagcag tcctcttcat actgcacatc cacatacgtc tgcggcccct
     5581 tcgtcgtcgc atagtaaatc ttcgcccccg gatttctctc cagaaaatcc tcgtaactct
     5641 catacaccgt cacatccagc ttcggccagt aatccagccc cgcgcgcctg accgccttgt
     5701 ccgaaatatc aaaccccagc ggcttaatca gatgcagcct cgtccccgtc gccacacacg
     5761 tccgtccgat attacctgta tttgctggaa tctccggctc atgaagaaca atattcaaat
     5821 gtgccatttt accaatcact cctgtcgtat atttaagcct tatctgccaa cccggtacat
     5881 ctcatccatc tgcggcttat ccagataccg gacctcttcc ccgttccgga gaatcctctc
     5941 ctttccggat gtcgtccgcc caatcaccgc cgatggaatc ccttcgagtg ccattttctg
     6001 caccagcccc tcgccgtccg ccgccgcaat cagcagcgcc cccgtcgaaa gcatctgata
     6061 cggattcacc ccgaaaaact cacagatctc caccgtctcc tgctggatcg gaatcctctt
     6121 cagcgccaca tcaagcccga tacccgcgcc gtccgccatc tcccagagcg ccccgaaaat
     6181 cccgccctcc gagagatcct gcatcgcagc cacaccgtgc tcgatcgcaa tctccgcctc
     6241 acggcagaca gagtaatcct ccgaaaaatc cgccgcccca tggataaatc ccggagaaaa
     6301 tctcgtcaga agctcatccc ttcttgtctt tgccagataa gacaccgcgc caagcgcaat
     6361 ccccttcgtc acgacaagat ccaaatccgc tgtcacacac gacggatcaa acagccccgc
     6421 acgcgttgtc ccgcctgccg ccgtcacggt aagcaccgga tacaaaaccc cgtccgtcac
     6481 ctccgtatgc ccgccgatca ccgcagttcc ataccgcaca cacgcaccgt caaccgcccg
     6541 catcagctcc tttaaatcct tttcttccat cgcctccggc agcatcagcg acaccagcac
     6601 tccttttgcc gatgcccccg ccgcggccac actgttgcac acccggcaga ccgcgagcac
     6661 cgcgtcctcc ggatccttca ccagcaccgg cgcacacgaa aacacctgcg tctccttttc
     6721 tcctgcaagt gccgataccc cgcgtccgac cgccggccct gctgcgtcct ctcttttctg
     6781 ccccagcatc ttaaacaccg agcgcaccag cacgctctgc tgtaatttac cctttcgcat
     6841 cccttgtcct cctcgaactt cctgtttcaa acgaaaaagc agacggaatc cgtactcctt
     6901 ttccctctgc tttcccattt ttcacggaaa atgttacagt tcactggttc ccagcgaatt
     6961 gtaaccagaa aacgcctagt cctcatcgtc ctccgttttc tctgtatcgc tgctgtcgtc
     7021 cgtcttttcc gtaccatccg aatccgaatc atcgctgctg tcttccttct tctccgaaga
     7081 actcgatgac gaactgctct ggctgtattt cgctgcaact tcctttgctt ttgcaagatc
     7141 atttgccgca atcgccgcct gcatttccgc acgcgccgct gcattgtccg tctttgttcc
     7201 cacggtatag acatccgatg tcatgcggta agtgctggaa ttcacctgct ccttcgtctc
     7261 atttccattg tcattcacaa tcttccacag ccgcgccgtc gaaccctgat agccgtacga
     7321 tgtctgttca attgttccaa acgatgcatt cgcatcttcc gtcaggctcg gcgtcggatc
     7381 ctccgtgctt gtcgtctcac tctcatagcg gaccgtgcgg ttcggatctc tcgtctcatg
     7441 gccgtagatg gcaaaatgca gggtgcttcc gtccgcgctc gcctcaatat aaatcggcgc
     7501 atccgtattg ttgatgaatt tcaggtcttt caatccctct gcaatcgccg catccatcga
     7561 cggatccaca tagtggacaa tcatggaatg gctgtaccgc tccgtcacct caagctctgc
     7621 ttttaagaca gcaacataga gcgttgtgga aacctggcag ataccgccgc cgtacgaatc
     7681 gacgaccttg cccatctcat acgatgctgc cagtgcataa ccattttccg catcaaacgg
     7741 cacaacgacc gcctctacgg aaaattcctc gcccggctgt accagcgttc cgttaatctt
     7801 ctctgcgccg actgcgacat tctgttttct gccgctcgta ccgttgccat actccgtcat
     7861 accctcgccg agcagatctt tgacaagtgc aagctgctcc ggattgccgc ctgccggtgt
     7921 ttcttctgtt accagttcta cgccgccgat gccgccccgc cagcttgtcg tcaggtaatt
     7981 gacaaccttc gccgtggact catccacgtt gacgctgcat ccggtctggc ccggattgac
     8041 gataaattcg ccgttttcgc gggtcagcgt tgcatctgtt gcctcgcggt tcagcacggc
     8101 cgtgttgttt tctaccgcct ggcgcgctgc ctcgccatcg actgtatatt tcagggaaaa
     8161 cgtcaccgtc tctccatttt tgagccggtt ctgtgtcagg aaccgcttca gtacattgcc
     8221 ttcctgcccg attgtgacgg cttctcttgc cacctccggg ttggcgcagg aaagtccgag
     8281 atcccctgct gtcacctgtg cattctgctc tccggcagac aggttgataa ccgagggttt
     8341 taactgttca attttgtcgt ttactgcctg tgtgatctgc tcttcggtca taccgccgac
     8401 ggaaatgtct tctacataga ttccgtccag cgctgtccgg tcatccgctt tgactggttc
     8461 tgccaaaaag cagaatgcga atgcgataag catcaccagc aggcctctca tttttctgct
     8521 gtttttcata ctgtgcctct tcttgatccg tttcccattt cactctgtct tttacagaat
     8581 ctctttacac cagccataaa agtgctggaa gaaccatacc aagcaccata accactgcaa
     8641 gaatcaatgc cagcatcttt gctgcatttc tcttctttgc cagcggatct ttcttctttt
     8701 tctgtgcctc tgtcggggct tttcgattga ctttattact catcgttcca tcctttcttt
     8761 ttctcttcta ttttttctca gttctctgtc gtttttccat tcttccatgt gccagaataa
     8821 tctgatcgat catgcgggat gcacggcttt ttgtgagtgc tccccactcc ggttcgagat
     8881 ccagctcatg gtaccggatc aggtccgcca gatatttttt ctgtgcctcc gtcgccggaa
     8941 cctcgcgttt cattttggcc ttcagaggcg ctgcggcaaa ccattccggg tgatcttccg
     9001 aaaatccttc ttccagtttt cggaacagtg ccagcgtttc cagcacatcg tccattgcac
     9061 ggtgtgcgtg ttcccgcggg atctgatagt agccgcagag tgcttcaagg cttctgctct
     9121 ccagatccgg gagggctttc cgtgcaattt tcaacgtatc ccacgcttct ttttcaaagt
     9181 ccagacgggc atttatcgct gcctgcttga tgaagctgta atcgaacaga atattgtgcc
     9241 ccagaagcgg caggccgccg cagaaatcca gaaagccgcg gacagcagtc tccacatcgg
     9301 gcgcattcgc aatcatctca tcccggattc cggtgagttc cgtgatccgc tccggcactt
     9361 ttctgcccgg attgacgaaa gtctcatacg atgccgtgac ctctccgccc tgcactttta
     9421 ctgcaccgat ctccagaatc tggtcttcct ttacagaaag acccgttgtc tcaagatcga
     9481 gcactacaaa atcttgtatt ttcatctttt attacctttt ctagtatctc atgttctttt
     9541 gaaatgtcaa gcgtaaaaaa gctcactgca gtcgatcatc tccgcatttc ccgtcagcgg
     9601 atcgcgcttc tgataatcaa acaggagtac cgtacgcgcc ttttccccgt ttccgagcac
     9661 atcgtaatca aaaacttcca catgcgtcat gcgctgcagc tgcttccaat cgtatgtcgt
     9721 atacgatggc agaagtgttc tcgtttcggc ttctttctga taaaagtcac gcgactcgtt
     9781 tttccattcg gacggatctt ttgcaaagcg cggccgtgtc ttcatatttt cacgcgcata
     9841 cagatcgagc accatcatct gacggaagaa tgcttcgtcc tgctgttttt cgtcctgcaa
     9901 aaactcgagc agaatttcat accgctgaat ccggttatgt gccgcctcac tgtagccctt
     9961 tgcccggtag aaatcgccga gctcctggaa gaaatcgaac gggttctcat acagacgctc
    10021 gatcatcggc agtgtgtgga cgaactggcc gctgttgtag tagacttcca ccatctcctc
    10081 cacgtctttg agcttcagca catcgtcgta cggcagccat ttcgtgtaca gtacctcata
    10141 tggttcgcgc tcctgataat gacagtcata ttcctcggta tgctcataca tgaaagaacc
    10201 gcgcagcact ttcaaaaatc ccagctgtaa ctgctgcgga cgcagtgcat agacatccgc
    10261 aaacgaacgg cggaaactgt cataatcctc atacggcagg cccgcgcaca cgtcggcgag
    10321 caccgacagg tcgtggctga tgatcaggag cccgacgccg aggtccttca ccaggcccgc
    10381 gatcaggtcg agatgctgat ggatgtttct tcccttcgca atccggttta caatctctgt
    10441 gactcttcca aaatccatct tccggtggat cgcctcgatc gtctgcggat tcgtagactg
    10501 aacgccgatc tcaagctgta ccagccccgg acgcagggta tttaagagtt tcagttcctc
    10561 ctcagtaatc agatcagcgg caatctcaaa gtggaaattc gtcacgccgt tgtcatgctc
    10621 cgcaatgaat ttccagatcg ccattgcgtg gtctttcttg cagttgaacg tacggtccac
    10681 aaatttgacc tgcggtactt tctgttccag gaaaaaggcg agttcctttt tgaccagatc
    10741 cagatttcgg aaccgaagcc gcttatcaat agaagaaaga cagtaactgc atgaaaacgg
    10801 gcaaccgcgg cttgactcat aataaatgat gcggttctcg aattttttca aattcgcata
    10861 cagaaaatcc acctcggaca gatccatgac cggacgccac gggttgcttt tgatggctcc
    10921 ttctgcatca cggtatgtaa tgccgtggat ctcttcaagc gttttcccct ccgtgccgtt
    10981 ctcctgattc tggtaataca ctgccagttc acggaaggta atttcgccct cacccttcat
    11041 gattccgtca aggaacggat actgctccag catctcctcc gcgtggtacg acacctccgg
    11101 cccgccaagc cagatcttcg tttccgggct gattttgtga tattccgtaa tcaaatcttc
    11161 cacaatagat atgttccaga tataacacga cacacagacg acatccgcat gcgccagaaa
    11221 cagactccgc agcatggaat cttttgactg gttgatcgta aactcacgga tctccgggaa
    11281 cacaccggct ttctccgcgc tcgcctgcag cgtgtaaact gcaagattgg aatgaatata
    11341 ttttgcgttg accgcagtca gaataatttt cataatcttt ttaatacttc tccttgcttc
    11401 ctctttgcat atcttttata ctaccacatt ctcgaacgaa aatacaccgc cattccccca
    11461 aaagcgcaat tttttcacga ttttcaacaa aacagttgca attttcatga attcatgtta
    11521 tattaacaaa ggtttccaac aggaaatcca aatgcccaga tagctcagtt ggtagagcag
    11581 aggactgaaa atcctcgtgt cg
//
LOCUS       DAOMUF010000056        11578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1324_length_11578_cov_2.983511, whole genome shotgun sequence.
ACCESSION   DAOMUF010000056 DAOMUF010000000
VERSION     DAOMUF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1324_length_11578_cov_2.983511"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLN77_04820"
     CDS             complement(<1..686)
                     /locus_tag="PLN77_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HRM43633.1"
                     /translation="MEEKLYDYMDWAEIEAVVYSEENHPRDILGPRVTEDGILIQAFF
                     PGATRAAVHTIRDGKQTEMVCEDEAGFFAVLLPGKKIPSYTLIYWDGDGNQMEHYDPY
                     AYPMQFTEQEQKQFENGICYSIYEKLGAHPVKIDGISGVYFAVWAPNAIRVSVVGNFN
                     NWDGRAYQMNLLESGIYELFIPGVRAGDIYKYEIKARGGLTYLKSDPYANAAQLRPNN
                     ASVVFDLGKYA"
     gene            complement(980..2215)
                     /locus_tag="PLN77_04825"
     CDS             complement(980..2215)
                     /locus_tag="PLN77_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43634.1"
                     /translation="MGRTVKDPNRRQPKPVQKVQLSEKNVGRRIVLVVLFLAIGSGFL
                     VYGFMNFLRGDSGWREISVKAGSELNCSEDFTLKYNVGAGGVSAGGEAKALSLIYTDA
                     AVKGYRLFNIDESFDDVTNLYDINQHPNEVMTVDPVLYDALKKVSDANCREIYLGPLY
                     ASLENLCMSNDDAVAAQFDPEKDDDAAEEAAAVAAFAQNPDDISMEFPGENQVCLHVS
                     DAYQAYAAEMGYTAYLDFFWMKNAFLIDYLADTIRGEGYQLGIISSKDGFVRCLDETG
                     EKEYQYPLYHLSGNEIQSHGTMTYEGPKSIVFFHAYQAGSPDTYRYYQYQDGTMRTPY
                     LSASDGKDHTAASELLVYSGEYGCADTLLAAFFDYQAESLSGESLKTLASQKIYSVWF
                     ENNEIQTTDEKFSVTAVNK"
     gene            complement(2370..2540)
                     /locus_tag="PLN77_04830"
     CDS             complement(2370..2540)
                     /locus_tag="PLN77_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43635.1"
                     /translation="MLSFFFPIIGIIAAMVYKKKQYYHNYKACKQGALYGFAVLAVIL
                     LIFIIALILVVL"
     gene            complement(2630..3613)
                     /locus_tag="PLN77_04835"
     CDS             complement(2630..3613)
                     /locus_tag="PLN77_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM43636.1"
                     /translation="MDENGREILLQVKNVDITFGKGDDAVKAVKNASFDIYKGETFSL
                     VGESGSGKTTIGRAVIRVNPCSAGEILYKGVKISGKIPHSLDREVIRNIQMVFQDPAA
                     SLNERATVDYIVSEGLYNFHLYENEADRVKKVENMINEVGLLPEHLTRYPHEFSGGQR
                     QRIGLARAMVMEPELVVADEPISALDVSIRAQVLNLLKKFQREKQVTYLFIAHDLSIV
                     RFISDRIGVIYKGNIVEVADAEELFNFPLHPYTHSLISAIPIPDPQLEKNKVLYTYDP
                     SIHDYSVDKPEFVDIGHNHFVYGNKKEIEEYKALREKGEPLQPPSASRVRK"
     gene            complement(3855..5603)
                     /locus_tag="PLN77_04840"
     CDS             complement(3855..5603)
                     /locus_tag="PLN77_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM43637.1"
                     /translation="MEDKKVLLSIKDLQVKFRVRGRILTAIRGVTLDIYENESIAIVG
                     ESGAGKSVFTKAFAGMLDSNGFIDQGDIIFNDAELSDTVVPLNSYAKKTIASTWEKLN
                     DYSKLEYGSEVFLKMKALEQEKEEKMTLSEEEREKADAEIKELVIKRTELFNYKQTLD
                     TSKEKAKIKETSAEISRLDGEIKALQKAKEEKIKAHKQAAMNDTAYNQAYDAKMAEYK
                     KEYAGLTAKEITDETRKRNEILAKEIYLSVGRYKLRKKVRMIKKLHEAFKAAMERGVD
                     LNDEQKRNGVFDQATFRVRYLDETPEQLHGTCIINLAKIQDPNDWGQIRGKKIATVFQ
                     DPMTSLNPIITIGKQITSVIMKHQDVSEVEARAQALELMEKVGIPNAEQRFDDYPFQY
                     SGGMRQRIVIAIALSCRPKILICDEPTTALDVTIQAQILKLIKDLQKEYNYTIVFITH
                     DLGVVANIADRVAVLYAGQIIEFANVEELFYDPRHPYTWALLSSLPQLAERNTKLFSI
                     TGTPPSLYNKIIGDPFAPRNQYCLKIDTLEEPPMFKVTDTHYAKTWLLDPRAPKTEKP
                     EAIQNIHEKLLKAYNL"
     gene            complement(5617..6585)
                     /locus_tag="PLN77_04845"
     CDS             complement(5617..6585)
                     /locus_tag="PLN77_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43638.1"
                     /translation="MNYDLDKFGLSEEELFSPVDNSSLESEKITAPRYSYWHSVFRVF
                     FKKKINIAVLCLLGVILLMTYVYPLFVEYDRFANLMNGATKHLSPGKALQQLGFNIHW
                     ILGSGASGQSTFDAVWFGSRISVSLAFICALINLSIGVIVGSVWGFSKKVDVFMMEVY
                     NIIGNIPYVLLISVLVLIFSPSFWTMVFALTVTGWLAIAYFIRTQVVIIRDREYNLAS
                     KCLGTSIFRIAMKNILPFMTSVIVTLAATEIPSYISYEVFLAYIGMGLSDMSLGRLIY
                     EAEGAMLTPGWGFEFWSPVVVASIITVVLYVVGQNLGDASDPRTHM"
     gene            complement(6592..8163)
                     /locus_tag="PLN77_04850"
     CDS             complement(6592..8163)
                     /locus_tag="PLN77_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43639.1"
                     /translation="MTKYLFKRLLHGLVSVVIVVGCVMTMIYSLMDRNLIFAQDTTYS
                     HQSNNQKKVYKYQKWETYGYLDYVTYADYLNALKAKGEIDEDTRSKAVAFGRTKDQDS
                     ELVSEYVKEFTAYYKAKGYTVRRYDAIMMGKKKYANGGQQQLFAYKDIPLATRLGKYF
                     SGLIDIDSIHDAAKVEGKRGITFTLHDPVYGGKKFSPAIMGNGTTHKYLMYFDSKFPY
                     FHQNIITINLGKSYSVNEGVDVALTMTRAQGSYVKSEITYPSGLVEYSADDLHSATYM
                     NESQDASAVHSDRFADDYTNVDTVKSGKSKIGYSFVIGIIAVILSYLIAVPLGILMAR
                     KKDKLVDQLGTIYIVFIIAVPSLAYIFLFKAIGGKMGLPTTFDMDSKSKLMYILPIVS
                     LALPQVANLMKWLRRYMIDQMNSDYVKFARSGGLTEGEIFTKHILKNAAIPIVQGVPA
                     SVLFALTGAIITERVYVVPGAGNLLTEAISKYDNGVIVGVTLFYAVLSVISIILGDVL
                     MSMVDPRISFSTKDR"
     gene            complement(8283..10496)
                     /locus_tag="PLN77_04855"
     CDS             complement(8283..10496)
                     /locus_tag="PLN77_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43640.1"
                     /translation="MKRKIVSALLVMSMTASLVACGNSGSGSSDPADESVSVSSSASS
                     ESDASASASSESEAEAEESEMPEGYDETSTELYNEALGDFNDALATAKEAETVDERYA
                     LMAVAEGKLMESAMMYPLTSRGGTYAMYRMAPRTKDYTLWGSDNDRYHQYVVTTDFIK
                     AEDYNEMRAKWDELKGTGTYESWVKEYLAGKGYTLKDSFSMPYASDPVTWDGLATSRA
                     ADTDAIINTYDGLMEYDVEGTLQPALAESYEVSDDGLTYTFHLRKDVKWTDSQGREVD
                     TVKADDFVAGMQHMCDAQGGLEYLVQGVIKNVSQYISGEVTDFDEVGVKAVDDYTVEY
                     TLEEPCSYFMTMLGYTIFMPMSRSYYQSQGGKFGAEYDSSAADYQYGKDSNSIAYCGP
                     YLVTNATAKNTIVFKLSDSYWNKDNVNIKTLTWLFNDQSDVTKMYTDAKAGTVDYVNL
                     NTSTMETAKSEGLYDQYAVVSDTDATSFMGFYNINRTATANANDGTTAKSTKSDEEIQ
                     RTNKALQNVHFRRAISFAADRGAYNAQQVGEDLKYTSLRNTFTPGYFVSLSKDTTIQI
                     NGTDTTFPAGTYYGEIVQKQIDADGVKIKVWDAENKTSDGFDGWYNPENAVEELNTAI
                     EELAEEGITIDESNPIQIEYPYPSAVEVYTNKANSYKKSVEAALGGKVVINLVDAVDM
                     DGWYYAGYYVNYGYEQNYDVYDVSGWGPDFGDPCSYLDTMLPDYEGYMTKCFGIF"
     gene            11173..>11578
                     /gene="cysS"
                     /locus_tag="PLN77_04860"
     CDS             11173..>11578
                     /gene="cysS"
                     /locus_tag="PLN77_04860"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321569.1"
                     /note="catalyzes a two-step reaction; charges a cysteine
                     by linking its carboxyl group to the alpha-phosphate of
                     ATP then transfers the aminoacyl-adenylate to its tRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRM43641.1"
                     /translation="MYLYNSATHKKAEFQTHTPGRVEMYTCGPTVYHFAHIGNLRSYI
                     MEDVLEKALRYEGYNVNRVMNITDVGHLSSDADTGEDKMVKGAKREHKTVMEIAQYYT
                     DAFFADCKKLNIRRPDVVQPATGCIDEYIKIIE"
BASE COUNT         2776 a         2917 c         2526 g         3359 t
ORIGIN      
        1 gcatattttc caaggtcaaa cacaacactt gcattgtttg ggcgcagctg tgccgcattt
       61 gcgtacggat ccgatttcag atacgtcaga ccgcccctgg ccttgatttc gtacttatag
      121 atgtcgcccg cgcggacacc cggaataaag agttcgtaga tcccggactc cagaagattc
      181 atctgatatg ctcttccgtc ccagttattg aagtttccga caacgctgac acggatcgca
      241 ttcggtgccc agacagcgaa gtaaacaccg ctgataccat cgatctttac cgggtgtgca
      301 cccagcttct catagatgga ataacagatg ccgttttcaa actgtttctg ttcctgctcg
      361 gtgaactgca tcggataggc gtacggatcg taatgctcca tctggtttcc gtcgccgtcc
      421 cagtagatca gcgtatagga cggaattttc tttcctggaa gcagcacagc gaaaaatccc
      481 gcctcatcct cgcaaaccat ctcggtctgt tttccgtcgc ggatcgtatg tacggctgca
      541 cgggttgcac ccgggaaaaa cgcctgaatc aaaataccat cctccgttac gcgcggtccc
      601 aagatgtctc ttggatgatt ttcctcggaa tagaccacgg cttctatttc cgcccaatcc
      661 atataatcgt ataatttttc ttccattttc aaaattataa cccctccttt tttattttac
      721 gtgctctcgc tgctgcttcc cccatacaaa atcgcataaa aaagcactca ttcatgccag
      781 agcgtgtttg aaaaaagctt ttcgtgagct gcgagtccca ttttcagccc aaacggtctg
      841 cagcaaccgg ataaggtcaa aacagatcgc gaagtggggc ttgcagattg tggaaatgat
      901 ttttcaaatt ctgattttat tctaccatcc taaccgcgat tgtacaatgc aaatctggtt
      961 tgttttttat tctgtttttt tatttattca ccgctgtcac ggaaaacttt tcgtctgtgg
     1021 tctgaatctc attgttctca aaccagacac tgtaaatttt ctgtgaagcc agtgttttca
     1081 gggactcccc ggaaagcgat tcggcctgat aatcgaaaaa tgctgcaagc agcgtatcag
     1141 cacagccata ttcaccggaa tagacaagca gttcagatgc tgccgtgtgg tcttttccgt
     1201 ctgacgcgct aagatacggc gtgcgcatcg ttccgtcctg atactggtaa tagcggtacg
     1261 tatcgggact gcccgcctgg taagcgtgga aaaatacgat gcttttcggt ccctcgtacg
     1321 tcatcgttcc atgtgactga atttcattcc cggaaagatg gtacagcggg tactggtact
     1381 ctttttcccc tgtttcatcg agacagcgca caaatccgtc cttgctggaa ataatgccga
     1441 gctgatagcc ctcgccgcgg atcgtgtccg ccagatagtc aatcaaaaaa gcatttttca
     1501 tccagaaaaa gtcgagatac gccgtgtagc ccatctctgc ggcatatgcc tgatacgcat
     1561 ccgacacatg caggcagacc tggttttctc ccgggaattc catggaaatg tcatccgggt
     1621 tctgcgcaaa ggctgcgacg gctgccgctt cctctgccgc atcatcatcc ttctccggat
     1681 cgaactgtgc tgcgaccgca tcatcattgc tcatgcagag attttccagt gacgcgtaca
     1741 gcggtcccag atagatttcc cggcagttgg catcggatac ttttttcagg gcatcgtaca
     1801 aaaccggatc taccgtcatc acttcgtttg gatgctggtt gatatcgtac aggtttgtca
     1861 catcatcgaa actctcgtcg atattaaaaa gccggtagcc cttcaccgcc gcatcggtat
     1921 aaatcagcga gagtgccttt gcctccccgc ccgccgatac gccgcctgca ccgacattat
     1981 atttcagtgt gaaatcctcg ctgcagttca gctccgagcc cgccttcacg ctgatttcgc
     2041 gccagccgga atcgccgcgc agaaaattca taaaaccata caccagaaaa ccggatccga
     2101 tcgccagaaa cagtaccaca agcacgatgc gccgtccgac attcttttca gagagctgaa
     2161 ctttctgcac cggcttcggc tgccgtctgt ttggatcttt tactgttctt cccattcttt
     2221 tactcctctt actttaagtt ctccccttca ttcgcactgt gttttattgt cccagaaaat
     2281 atatctcccg tcaagtaaaa cggcacaaaa aagcagcgcc ggaatcaaaa gactttcgat
     2341 tctgacactg ctttgttttt cacagtctgc tataggacaa ccaatatcaa tgcaatgata
     2401 aagatcagga gaatcacggc cagtacggcg aatccgtaca gcgcgccctg cttacatgct
     2461 ttatagttgt gatagtactg tttctttttg tataccattg ctgcgataat accgatgatc
     2521 gggaagaaga acgacagcac cttgtaccag atgcttccgg tatcgtgaag cggagttttc
     2581 aggaactcat cttctgcctt ggagaaatga acctcgtggt cctcctcttt tacttcctca
     2641 cccggcttgc ggatggtggc tgcagcggct ctcccttttc acggagtgcc ttgtactctt
     2701 cgatctcttt cttgtttccg tatacaaagt ggttgtgtcc gatatccacg aactccggtt
     2761 tgtccacgga ataatcgtga atggacggat cgtaggtgta cagcactttg ttcttctcaa
     2821 gctgcggatc cggaatcggg atcgcagaga tcagggaatg ggtgtatgga tgcagcggga
     2881 agttgaacag ctcctctgcg tccgcaacct ctacgatatt tcctttgtag ataacaccga
     2941 tacggtcgga aatgaaccgt acgatggaaa ggtcatgggc gataaacaga taagtaacct
     3001 gtttttctct ctggaatttc ttcagcagat tgaggacctg ggcacggatc gatacgtcca
     3061 gtgcggagat cggctcatcg gctacaacca gctccggctc cataaccatg gcacgggcca
     3121 gaccgatacg ctggcgctgt ccgccggaga actcatgtgg gtaacgggtc aggtgctccg
     3181 gcagaagacc aacttcgttg atcatgttct ctaccttttt cacacggtct gcctcgttct
     3241 cgtacagatg gaagttgtaa agaccctcgg atacgatgta atcgaccgtc gcacgctcat
     3301 ttaacgatgc ggcaggatcc tggaatacca tctgaatgtt tcggataacc tcccggtcca
     3361 gggaatgcgg aatcttgccg gaaattttaa cacccttgta caggatctct cctgcggaac
     3421 acgggttgac acgaatgacc gcacggccga tcgtggtctt tccggaaccg gactcgccta
     3481 cgagggagaa ggtttctccc ttgtagatgt caaaagaagc attttttacc gctttgactg
     3541 cgtcatcgcc cttgccgaaa gtgatatcta cattttttac ctgcagtaag atttctcttc
     3601 cattttcgtc aatcggacca tgctccggca gatgcgaaac agtggtcttg ctttcctgct
     3661 ttgtagacac tttcaaactc tcctattagt tgattcctgt aacaccagat tgcttcgcaa
     3721 tcttaaaaac tttcgcaatc cgctcatttt tctccgaaaa atggctactt gctcatgttt
     3781 ttgcaggtct caaactcaaa aaccctcgtg caagctttgc ttgcgtcggc ttttcgacct
     3841 tgatcccctt ggttttacaa gttgtatgct tttaataatt tttcgtgaat attctgaatt
     3901 gcctccggct tctccgtctt tggtgctctc ggatccagca gccaggtttt ggcgtaatgg
     3961 gtatccgtta ccttgaacat cggcggttct tccagtgtgt cgattttcag acagtactgg
     4021 ttacgcggcg cgaacgggtc tccgataatt ttgttataca gagacggcgg ggtaccggta
     4081 attgagaaca gctttgtatt ccgctccgca agctgcggca gggaagaaag cagcgcccag
     4141 gtgtacggat ggcgcggatc gtagaatagc tcttctacgt tcgcaaactc gatgatctgt
     4201 cctgcgtaca gaacggcaac acggtctgcg atattcgcta cgacaccaag gtcgtgggta
     4261 ataaacacga tagtgtagtt gtactctttc tgaagatcct tgatcagctt caggatctgt
     4321 gcctgaatcg taacgtccag cgcagttgtc ggctcatcgc agatgaggat cttcggacgg
     4381 caagaaagtg caatcgcgat aacgatacgc tgtctcatac cgccggaata ctggaacgga
     4441 taatcatcga aacgctgctc tgcgttcgga ataccaacct tttccatcag ctcaagcgcc
     4501 tgtgctctcg cttccacctc gctgacatcc tgatgtttca taataacaga agtgatctgt
     4561 tttccgatcg tgatgatcgg gttcagggaa gtcatcgggt cctggaatac ggttgcaatt
     4621 ttcttaccac ggatctggcc ccagtcattc gggtcctgga tttttgccag gttgatgatg
     4681 caggttccgt ggagctgctc cggtgtctca tccagatagc gtacgcggaa ggtagcctgg
     4741 tcaaaaacgc cgtttcgctt ctgctcatcg ttcagatcca caccgcgctc cattgcagct
     4801 ttgaatgcct cgtgcagctt tttgatcatg cggacttttt tgcgcagttt gtaacgtcca
     4861 acagaaagat agatctcttt ggcaagaatc tcatttctct ttcttgtctc gtccgtaatt
     4921 tcttttgctg tcaggccggc atactctttt ttgtactctg ccatctttgc atcatacgcc
     4981 tgattgtagg ccgtatcgtt catggcagcc tgtttgtgtg ccttgatttt ttcctctttt
     5041 gctttctgca gagccttgat ctctccatcc agtctggaaa tctcggcaga tgtctctttg
     5101 attttcgctt tttctttgga agtatcgagt gtctgcttat agttgaaaag ttcggttctc
     5161 tttattacaa gttccttgat ttcagcatct gctttctcac gctcttcctc gctcagcgtc
     5221 attttttctt ctttttcctg ctccagcgcc ttcattttca ggaatacttc tgaaccatat
     5281 tccagtttgg aatagtcatt cagcttttcc caggtggatg cgatggtctt tttcgcataa
     5341 gagttcagcg gcacaacggt atcgctcagc tcggcatcgt tgaaaatgat atctccctgg
     5401 tcaatgaagc cgttgctgtc cagcatgccc gcaaacgctt ttgtgaatac ggatttaccg
     5461 gcaccggact ctcctacgat agcaatggac tcattctcgt aaatatccag cgttacgccc
     5521 cggattgccg tcaggatacg gccgcggaca cggaatttta cctgtaagtc cttaatcgat
     5581 aataatactt ttttatcttc catgcggctt tccttcctac atatgtgttc tcgggtcgga
     5641 cgcatctcca aggttctgtc caactacata taatactacg gtgatgattg aagcgacaac
     5701 aaccggactc cagaactcga atccccatcc cggtgtcagc atggcaccct cagcctcata
     5761 gatcagtctt cccagagaca tgtcagaaag tcccataccg atgtaagcca ggaacacctc
     5821 gtaagagatg taggacggaa tctcagttgc cgccagagta acaatgacag atgtcataaa
     5881 cgggagaata ttcttcatcg caatgcggaa aatagaggtt ccgagacatt ttgatgccag
     5941 attatactct ctgtcacgga tgataacgac ctgtgtacgg ataaaatacg ctatggcgag
     6001 ccatccggta acggtcagtg caaataccat ggtccagaag ctcggagaaa agataagcac
     6061 caggacggag atcagaagta catacggaat atttccgatg atgttgtaaa cctccatcat
     6121 gaagacatca accttcttcg aaaatcccca gacagatccg acaatcacac caatcgaaag
     6181 gttgatgagc gcacagataa acgccaggga aactgaaatt ctggaaccaa accagacagc
     6241 atcaaaggtt gactgtccgg aagcaccgga tcctaaaatc cagtgaatat tgaatccaag
     6301 ctgctgcaag gctttgcctg gagacagatg ttttgtcgcg ccgttcatca gattggcgaa
     6361 acggtcgtac tccacaaaga gcggatacac atacgtcatc agcagaatca cacccaggag
     6421 gcaaagtacg gcaatgttga tttttttctt aaaaaatacg cggaacacgg aatgccagta
     6481 ggaatatctc ggagccgtaa ttttctcaga ctccaaactg ctgttatcta caggagaaaa
     6541 cagttcttcc tcagacagac cgaatttatc taaatcataa ttcataagtc cctacctgtc
     6601 tttcgttgag aatgagattc gtggatccac catggacatc aaaacgtcgc ccaggatgat
     6661 ggaaatcacg gagagcactg cgtagaacag tgtaacacct acgataacac cattatcata
     6721 tttgctgatt gcttcggtca gcagattacc ggcaccaggt actacgtaca cacgctcggt
     6781 gatgatggct ccggtcagtg cgaacagtac gctcgccgga acaccctgta cgatcggaat
     6841 cgctgcgttc ttcaaaatgt gtttcgtaaa gatttctccc tctgtcagtc caccggatct
     6901 tgcgaatttt acgtaatcgg agttcatctg gtcgatcatg tatcgacgca gccatttcat
     6961 caggtttgcc acctgcggca gtgccagaga aacgatcggc agaatataca tcagcttgct
     7021 cttcgaatcc atgtcaaagg tggttggcag acccattttt cctccgattg ccttaaacag
     7081 gaagatatag gccagtgaag gtactgctat aataaataca atatagatgg ttccaagctg
     7141 gtcaaccagt ttgtctttct ttctcgccat cagaatgcca agcggcactg cgatgagata
     7201 ggaaagaatc acggcaatga taccgatcac aaaggaatag ccgatttttg atttaccact
     7261 cttaactgta tccacgttgg tatagtcatc tgcgaaccga tcggaatgaa cagcgcttgc
     7321 atcctgagat tcgttcatat acgttgcgct gtgcagatcg tctgcactgt attccaccag
     7381 accgcttggg taggtaatct ccgattttac gtaggatccc tgcgcgcggg tcatggtcag
     7441 tgctacatcc acaccctcat ttacggaata ggactttcct aaatttatgg tgattatatt
     7501 ctgatggaaa tatgggaatt ttgagtcaaa gtacatcagg tacttgtgtg ttgtgccatt
     7561 tcccatgatg gccggtgaaa atttctttcc cccatatacc ggatcgtgta aggtaaatgt
     7621 aatccccctc ttaccttcta cctttgcagc atcgtgaatg ctgtcgatgt caatcaaacc
     7681 ggaaaaatat tttccgagac gcgttgcaag cggaatatcc ttgtacgcaa acagctgctg
     7741 ctgtccgccg ttggcatatt ttttcttacc catcatgatc gcatcgtatc ttcttacggt
     7801 gtaacctttt gctttataat aagctgtaaa ttcctttaca tattcagata ccagttctga
     7861 atcctggtct ttcgttcttc cgaatgctac tgctttggat ctcgtatcct cgtcaatttc
     7921 gccttttgct ttgagagcgt tcaaataatc tgcataggtt acataatcaa gatatccgta
     7981 tgtctcccac ttctgatatt tgtacacctt tttctggtta ttgctctgat gggaataggt
     8041 ggtatcctgt gcgaaaatca gatttcggtc catcagagaa tagatcatag tcataacgca
     8101 tccaacgacg attactacgg aaacaaggcc atgcaacaaa cgcttaaaaa gatattttgt
     8161 catattctct cattccttaa cacaatatga caggggaatg gctttttttg tccccatccc
     8221 cctgccggtt tattcattgg ctttcaactg ttctggtctt tccaatttta ccttttccaa
     8281 aattagaaga taccgaaaca tttggtcatg tatccttcgt agtccggaag catggtatcc
     8341 agataagagc acggatcacc aaagtccgga ccccatccgg agacatcata tacgtcgtag
     8401 ttttgttcat aaccatagtt tacatagtat cctgcatagt accatccatc catgtctacg
     8461 gcatcaacca gattgatcac aacttttccg ccaagggcag cctctactga tttcttgtag
     8521 gagtttgctt tgttggtgta aacttctact gcagacggat atggatactc aatctggatc
     8581 gggttgctct catcgatggt gatgccttct tctgcgagct cctcgattgc ggtattcagt
     8641 tcttctactg cgttttccgg attgtaccat ccatcaaaac catcactggt cttgttttcg
     8701 gcatcccata ctttgatctt cacgccgtcg gcgtcgatct gtttctgtac gatttcacca
     8761 tagtaggtgc ctgccgggaa ggttgtgtcg gttccgttga tctgaatggt agtgtctttt
     8821 gacaggctta cgaaatatcc cggggtaaag gtatttctaa gggaagtata cttcagatcc
     8881 tcgccaacct gctgtgcatt gtaagcgcca cgatctgctg caaagctgat cgctcttctg
     8941 aagtgaacgt tctgcagtgc tttgtttgtt ctctggattt cttcgtcaga ctttgtactc
     9001 tttgctgttg ttccgtcgtt ggcatttgca gtcgcagtac ggttgatatt gtagaatccc
     9061 atgaaggaag tagcgtcggt atctgaaact actgcgtact ggtcgtacag accttcggat
     9121 tttgctgtct ccatagtgga ggtgttcagg tttacatagt caacggtacc tgcttttgca
     9181 tctgtgtaca ttttggtaac atcagactgg tcattgaaca gccaggtcag ggttttaatg
     9241 tttacgttgt ctttgttcca gtaagaatcg ctcagtttaa atacgatggt atttttcgct
     9301 gtagcgttgg ttaccaggta cggtccgcag tatgcgatcg agttggaatc ttttccgtac
     9361 tgataatctg ctgcggaaga atcgtactca gcaccgaatt taccaccctg ggactgatag
     9421 tagcttctgc tcatcggcat gaagatggtg tagcccagca tggtcatgaa gtagctgcat
     9481 ggctcttcca gtgtgtactc tacggtatag tcatcaacag cttttacacc aacctcgtcg
     9541 aaatcggtaa cttcaccgct gatgtactgg gatacgtttt tgatcacgcc ctgtaccagg
     9601 tattccagac cgccctgtgc atcacacatg tgctgcattc cggccacgaa gtcatctgct
     9661 tttacagtat ccacttcacg tccctgggaa tcggtccatt ttacatcttt acgaaggtgg
     9721 aaggtatagg tcagtccgtc atcggatact tcataggatt ctgccagtgc cggctgcaga
     9781 gttccctcta cgtcgtactc catcaggccg tcataggtat tgatgatcgc atcggtatct
     9841 gccgcacggg aagttgcaag tccgtcccat gttaccggat cgctcgcata cggcatggag
     9901 aagctgtctt ttaaggtgta accttttcct gccagatatt cttttaccca gctctcatag
     9961 gttcctgttc ctttgagctc atcccatttt gctctcatct cgttgtaatc ttctgctttg
    10021 ataaagtcag ttgttacaac atactgatga taacggtcgt tatcgcttcc ccaaagtgta
    10081 tagtccttgg tgcgcggagc cattctgtac atcgcgtacg taccacctct tgaggtgagc
    10141 gggtacatca tggcggactc catcagtttt ccctcggcaa ctgccatcag ggcatatctc
    10201 tcatccaccg tttctgcttc ctttgcagta gcaagtgcat cgttgaaatc gcccagtgcc
    10261 tcgttataga gctctgtgct ggtctcgtcg tatccttccg gcatttcgga ctcctctgcc
    10321 tcggcttcgc tctcactgct tgcagatgcg cttgcatcac tttcggaaga cgcgctgctg
    10381 cttacgctta cactctcatc tgccggatca gaacttccgc tgccggaatt tccgcaggca
    10441 accaggcttg cagtcatact cataacaagt aatgcgctta caatttttct tttcataatt
    10501 cttcctaact cctttcatgg attcgcgttg tttttgcatc atccggacat ttgccgggac
    10561 ggcttcgcca ttccaccgtt tttcattttg gcactttacc acatcaaaac gaagagtaca
    10621 aaaaggagat gcgtccaaca agatttgttt tcttcatccc tcatgcccgt acatttgtcc
    10681 gtttgtccaa aaacaactgc attcattata accagaactc tctctgtttg tcaatatccg
    10741 atgcaggaga aaagtgtata aactgcattt aaagtcatca aaatcctcta ttttcctctt
    10801 tttgggtatt gttctgatgc cgtttttcta ttttcctgta ttcttcggtt ttataataaa
    10861 aaaaagatta cacaaataat tgtttctttc catttgcgtt ctgcagtact gtctttttat
    10921 ttctgaaaaa tgtatttctt cttattaaat gcgttttact ttttgtcttc gacagcctcc
    10981 ggactattga cagcaaaaaa aaatcctgct aatattaatt ttaataagga accagtgcag
    11041 tacacggaaa acaggcaccg cacgcggcgt ctctgatatc cttgtcggat acagcagacg
    11101 cttttttgct ttcctgtgta tcatcacttc tgcatccgta tccgcacgaa aagaaaaaag
    11161 gagacttctt ttatgtatct ttataattct gcaacccata aaaaagctga attccagaca
    11221 cacacaccgg gccgtgtaga aatgtacacc tgcggaccta ccgtttatca ttttgcgcac
    11281 atcggaaacc tgcgcagtta tattatggag gacgttcttg aaaaggcact ccgctatgaa
    11341 ggctacaacg tcaaccgtgt catgaacatt accgacgtcg gccatttaag ctccgatgcg
    11401 gacaccggtg aggacaagat ggtcaaaggt gccaagcgcg agcataaaac agtcatggaa
    11461 atcgcacagt attacaccga tgcattcttt gcggattgca aaaagctgaa catcaggcgc
    11521 ccggatgtcg tacagccggc aacgggatgc attgacgaat atatcaaaat cattgaaa
//
LOCUS       DAOMUF010000057        11399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1354_length_11399_cov_2.957158, whole genome shotgun sequence.
ACCESSION   DAOMUF010000057 DAOMUF010000000
VERSION     DAOMUF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 11399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11399
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1354_length_11399_cov_2.957158"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLN77_04865"
     CDS             <1..202
                     /locus_tag="PLN77_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitric oxide reductase activation protein"
                     /protein_id="HRM43642.1"
                     /translation="DYALHDTATEVRRLRQQGVSVLGVFVGEETELMAEQKIFGKDFA
                     YARDIRNFSHMVGSYLRRKIEE"
     gene            439..900
                     /locus_tag="PLN77_04870"
     CDS             439..900
                     /locus_tag="PLN77_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM43643.1"
                     /translation="MCCKKRKIGHELRTLDNMMSRNLMAAARERGVDELTAMHGWILG
                     YLCRNEDKDIFQKDIEAEFKICRSTVTNILKLMEKKGYIKRESVPYDARLKKLVLTDT
                     GRELHEKTKDMIDILEEQTIEGIAKEDLDTFYRVIDQLKSNVKNMLGETSC"
     gene            894..2642
                     /locus_tag="PLN77_04875"
     CDS             894..2642
                     /locus_tag="PLN77_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43644.1"
                     /translation="MLKTLGAQVKEFKKASIATPLFMILEVIMEMIIPLMMASIIDDG
                     VEKGDLHHIYVVGAFMVAAAAIGLFAGIMGGKYGAKASAGFARNLRQAMFENIQEYSF
                     SNIDKFSTAGLVTRLTTDVTNLQNAYQMILRMCMRAPSSMIIAMIMSFYINARLASVY
                     LVAILILGTCLFLIMRSAMKYFDQAFPKYDELNASVQENVSAIRVVKAYVREDHETSK
                     FHKAAENIYKIFCKAEENLSFNAPLMQLTVYSCILLISWLGAKMIVGSSLTTGELMSL
                     LTYCMNILMSLMMLSMVFVMITMSLASARRITEVLNEKSDLDNPENPDFDIPDGSITF
                     DHVYFNYKKGAGEPVLSDINLSIKSGETIGIIGGTGSAKTSLVNLISRLYDVTEGSIK
                     VGGKDVRSYDMETLRNEVAVVLQKNVLFSGTILDNLRWGDKNASEEECKRVCRLACAD
                     EFIDRMPEGYNTYIEQGGSNVSGGQKQRLCIARALLKKPKVLILDDSTSAVDTATDAK
                     IRKAFLTEIPETTKLIIAQRISSVQDADRIIVMENGKVNGFGSHEELLKTNEIYREVY
                     ESQTGGGGDFDEKRGE"
     gene            2646..4571
                     /locus_tag="PLN77_04880"
     CDS             2646..4571
                     /locus_tag="PLN77_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43645.1"
                     /translation="MAERVQQVKGPGARPPRGVKPQVKNPMKIFTRIMKYALKDYTVH
                     CIAVVIAIFVNVLANVQGTMFMKTLIDGYITPMLTQPDPDFGPLLHAILRVAGFYGIG
                     IVAAFIQNRTMIYVSQGTLRNLRNDLFGNMEKLPIKYFDTHAHGDIMSIYTNDIDTLR
                     QMISQSIPQIINSAITIVSVFVSMLILNIPLTITTLVMVSIMLGVTKKVAGFSGNFFV
                     KQQRDLGKVNGFIEEMMDGQKVVKVFCHEEENIEKFKEINNNLYESAYNANAFANVIG
                     PINAQLGNMSYVVCAIVGGALALGNVGGFTLGGLASFLTFNKSFNMPINQVSQQLNAI
                     VMALAGAERIFRLMDEEPELDEGYVTLVNAKEENGKLTETKERTGKWAWKHTHQADGS
                     VDYRELQGRVVFEHVDFGYNPDKIVLHDVSLFAEPGQKIAFVGSTGAGKTTITNLINR
                     FYDIQKGKIRYDEINIGKIKKDDLRRSLGIVLQDTHLFTGTVRENIAFGKLDATEDEV
                     VAAAKLANAHGFIMRLPQGYDTMLTGDGANLSQGQRQLLAIARAAIADPPVLILDEAT
                     SSIDTRTERIVQDGMDKLMSGRTTFVIAHRLSTVRNADCIMVLEQGQIIERGTHDQLM
                     EQKGKYYQLYTGNQVSA"
     gene            4812..6122
                     /locus_tag="PLN77_04885"
     CDS             4812..6122
                     /locus_tag="PLN77_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HRM43646.1"
                     /translation="MEYLNTAQELLDFIMRCKSPFHVVKEAGDLLNENGYTKLEETKE
                     WSLVPGGKYYVTRNESSVIAFQIPESAFSSYQIIASHSDCPTFKVKGEDPFVTDGHYT
                     RLNVEGYGGMIRSPWFDRPLSLAGRMVVRDGNSVKSVLVDAGRDLVTIPNLPIHLNRD
                     INNGIKYSVQTDMLPILGDETAKDHFYELFLPDTAKEDILSMELYLYNRVEGSIWGAS
                     KEFLSSGRLDDLQSAFGSLKGFLAAKATGQVNVCAIFDNEEVGSLTKQGADSSFLTET
                     LQHINAACGKDTIAYHRDLAGSFMVSADNAHAFHPAHAEKYDPKSRVYMNGGVVIKQS
                     ANQKYTTDAVSEAVTKMICEKAGVPCQVFANHADIPGGSTLGAILNSLVSVTSVDIGM
                     AQLAMHSPYETAGAKDTAYLVQFAKTFYETTLVRTEHGFFLEEK"
     gene            complement(6217..9807)
                     /locus_tag="PLN77_04890"
     CDS             complement(6217..9807)
                     /locus_tag="PLN77_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRM43647.1"
                     /translation="MLQIQHIKKEYRTGTLVQKALDDVSLNLRDNEFVAILGPSGSGK
                     TTLLNIIGGLDRYDSGDLIINGISTKKYKDRDWDSYRNHTIGFVFQSYNLIPHQTVLA
                     NVELALTISGVSKEVRRKKAIAALEKVGLGKQLHKRPSQMSGGQMQRVAIARALVNDP
                     EILLADEPTGALDSDTSIQVMDLLQEVAKERLVVMVTHNPELAELYANRIVTLRDGVI
                     CSDTNPFEPDSETLLPPKHKNMGKSSMSFLTALSLSFNNLRTKKARTLLTSFAGSIGI
                     IGIALILALSSGVNEYIQNMEEETLSEYPLQIQSTGFDLTSMMVGSQNAGKDSKDSGK
                     KNEIKVQEVVTNMFSTMNSNDLASLKKYLDGGADGISDYTNAIEYSYSVTPQIYREDG
                     DSIRQVHPDQSFRSLGLGASSNSIMSSMMSTNVFYEMPDTASLYENQYDLKAGHWPEN
                     YNECVLVLTSDGGMSDFLLYTLGLRDALELDEMVRQFLNEETVETPSSIGTYSYDDIL
                     GTTFRLVNSADCYEYDSQYQVWKDKSDNEDYMKKLVANGEKLTITGIVQPSPDSAASA
                     LTAGINYPASLTRHVVEEAKKSEIVKQQMADSSIDVFTNQEFGTVDQNSGLDMDSFIT
                     VDEDKLQNAFQFDDSALSGAFSGDSMNLDFSSAFADAGSSMDLAGAIDLSQISLNFDG
                     LPQLDLGQILSGLTLNVSEDGMQELASSLMEGYQGYASEHPEADYSHLQEDFVSYINS
                     TEAQEILRRHIQTIIAGGGVSITQEQIQELIQRIFKGYQAYLIKMGYTDPEQFDAYLA
                     EYLQTEEAVQIINDWISEVMQNNGNIEITSDQLTALATELSNGYLAYVKENGLPDPTE
                     MGQHFIAYLNTADGQQRLNSGITKIVDLDGLQSQVSSALTNYMAGVMGSYSASVASVL
                     QTQITSAMEQMMTQMASAIQSEMERAMTQVGNSLQDSMGDAMKIDTDAFASAFQMNMT
                     EDDMKELFASLSSSGSSTYEGNLQKLGYADFDEPSSISIYPKNFESKEHVVSILDGYN
                     DTMEKEGKNEQVISYTDMVGTLMSSVTDIVNIISYVLIAFVGISLVVSSIMIGVITYI
                     SVLERKKEIGILRAIGASKRNISQVFNAETFIIGLCAGLIGIGLSLLLMIPGNLLIHH
                     LADTTKVSASLPLVPALVLIALSVVLTLIGGLIPSKKAAKSDPVTALRTE"
     gene            complement(9997..10320)
                     /locus_tag="PLN77_04895"
     CDS             complement(9997..10320)
                     /locus_tag="PLN77_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM43648.1"
                     /translation="MRDKDVIQHIQDLCKERSWTYYRLAKEADIPYSTLNNMVNRTNI
                     PTIPTLQKLCDAFGITLADFFLDEPDALQLTEGQQEIITLYNNLSLEKKKILKAYMKG
                     LLMEV"
     gene            complement(10539..10907)
                     /locus_tag="PLN77_04900"
     CDS             complement(10539..10907)
                     /locus_tag="PLN77_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRM43649.1"
                     /translation="MSEELFNSASCSGDCADCGSDCQDLDQPTVTLTLDDDTEVKCAV
                     LTIFPAPNQKEYIALLPLDENGENEDGEVYLYRYYEENGQPNLDNIDSDEEYEIAADA
                     FDELMDTEQFNEMEENSTEE"
     gene            11186..>11399
                     /locus_tag="PLN77_04905"
     CDS             11186..>11399
                     /locus_tag="PLN77_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HRM43650.1"
                     /translation="MDIRFEKREHLQEKPDQKNLGFGKHFTDYMFVMDWDKEQGWHDA
                     AIVPYGGIPMDPATMVLHYAQETFEGM"
BASE COUNT         3054 a         2535 c         3027 g         2783 t
ORIGIN      
        1 tgattatgcg ctgcacgaca cggcaaccga ggtgcggcgc ctcaggcagc agggtgtctc
       61 ggtgcttggg gtctttgtag gcgaggagac ggagcttatg gcggagcaga agattttcgg
      121 caaggatttc gcgtatgcgc gcgatatccg caacttttcc catatggtgg gaagctattt
      181 aagaagaaaa atcgaagaat aaacgagcgg aatgctccgg gcggaaagtg aaaaactttt
      241 atccggagca tttttttcaa attttttacg caaacaagtt gacatttccc acaaaacctc
      301 ctagaatgtt ctatagcgaa ctgtttaata tgaaacagtc atctatagaa cagttgctta
      361 tagaacagtc cttattcaaa cagttttata acgaacagat ggggacggca gcaaagggga
      421 aaggaggaaa aaagcagcgt gtgttgtaaa aaaagaaaaa tcggccacga actgcggacg
      481 ctggacaata tgatgtcacg gaacctgatg gcagccgcca gagagcgggg ggtcgatgaa
      541 ctgaccgcca tgcacggatg gattttaggg tatctgtgcc ggaacgaaga caaagatatt
      601 ttccagaagg atatcgaggc ggagtttaag atctgtcggt ccaccgtgac gaatatttta
      661 aagctcatgg agaaaaaggg ctacatcaaa cgggagtccg ttccctatga cgcgcggctg
      721 aaaaaactgg tgctgaccga taccggaaga gagcttcacg agaagacgaa agatatgatc
      781 gatattctgg aagaacagac aattgaagga attgcaaaag aagatctcga taccttctac
      841 cgtgtcatcg accagctcaa aagcaatgta aaaaatatgt taggagagac atcatgttaa
      901 aaacacttgg agcacaggta aaagagttta aaaaagcctc cattgccact ccgcttttca
      961 tgattctgga agttatcatg gagatgatca ttcctcttat gatggcatcc attatcgatg
     1021 atggtgtaga aaaaggcgat ctccatcaca tctacgtggt gggagcgttc atggtggcag
     1081 cagcagccat cggtctgttt gccggtatca tgggcggaaa atatggagca aaagcgtctg
     1141 ccggatttgc aagaaatctg cgtcaggcca tgtttgaaaa cattcaggaa tattcatttt
     1201 cgaatatcga caaattcagc accgcaggtc ttgtcacccg cctgacgaca gacgtcacca
     1261 acctgcagaa tgcctatcag atgattcttc gtatgtgcat gcgtgcaccg tcgagcatga
     1321 ttatcgcgat gattatgtcg ttttatatca atgcacgtct tgcaagcgtt tatctggtag
     1381 ccattcttat cctcggaacg tgtctgtttt tgatcatgcg ttccgcgatg aaatatttcg
     1441 atcaggcatt tccgaagtac gatgagctga atgcgagcgt acaggaaaac gtatccgcca
     1501 tccgcgtagt aaaagcgtat gtgcgtgagg atcatgaaac aagtaagttc cacaaagcag
     1561 cagagaacat ctacaaaatt ttctgtaaag cagaggaaaa cctttccttc aacgcgccgc
     1621 tgatgcagct taccgtttac agctgtattc ttctgatcag ctggctcggt gcgaagatga
     1681 tcgtcggaag cagcctgaca acaggagagc tgatgagcct tctgacctac tgcatgaaca
     1741 ttttgatgag cctcatgatg ctttcgatgg tattcgttat gatcacgatg agccttgcga
     1801 gtgcgcgccg tattaccgag gttctgaatg agaagagcga tctcgacaat ccggagaatc
     1861 cggactttga tattccggac ggaagcatta ccttcgatca cgtctacttc aactacaaaa
     1921 agggagcagg cgagccggtt ctttcggata tcaacctttc gatcaaatcc ggtgagacca
     1981 tcggtatcat cggcggaacc ggaagtgcga agacgagtct tgtcaacctg atcagccgtc
     2041 tttacgatgt gacggaaggc tccatcaaag tcggcggaaa ggatgtccgt tcctacgata
     2101 tggaaactct gcgaaatgaa gtagccgttg ttttacagaa aaatgtgctg ttttccggta
     2161 ccattctcga caatcttcgc tggggtgata aaaatgcttc ggaagaagag tgcaagcgtg
     2221 tctgccgtct tgcctgcgct gatgaattta tcgaccggat gccggagggc tacaacacct
     2281 acatcgagca gggcggatcc aacgtatccg gcggccagaa acagcgcctc tgtatcgcac
     2341 gagcgctgct gaaaaaacca aaggtgctga ttctggatga cagtacaagt gctgtcgata
     2401 ccgcaaccga tgcaaaaatc cggaaggcat tcctgaccga gattcctgaa acgacaaagc
     2461 tgatcattgc acagcgtatt tccagtgtgc aggacgcaga ccgtatcatt gtcatggaga
     2521 acggaaaagt aaatggtttt ggaagccatg aggaattact gaaaaccaat gaaatttacc
     2581 gggaagtata tgagtctcag accggtggcg gcggagactt tgacgaaaag agaggtgagt
     2641 aaggaatggc agagagagta cagcaggtaa aggggccggg cgcaagacct ccaagagggg
     2701 tgaaaccgca ggtcaagaat ccgatgaaga tctttaccag aattatgaaa tatgcattaa
     2761 aggattatac cgtacattgc atcgctgttg tcattgcgat ttttgtcaac gtcctggcaa
     2821 acgtacaggg aacgatgttt atgaaaacgc tgattgacgg ctatatcacg ccgatgctga
     2881 cccagccgga tccggatttt ggcccgctgc tccacgcaat tttgcgggtg gccggatttt
     2941 acgggatcgg tattgtggct gcgttcattc agaaccgtac catgatttat gtgtcccagg
     3001 gaaccctgcg gaatctgaga aatgatctgt tcggcaatat ggaaaagctg ccaatcaaat
     3061 attttgatac gcatgcgcac ggcgatatta tgtcgattta tacaaatgat attgatactc
     3121 tgcgccagat gatcagtcag agtatcccgc agatcatcaa cagtgcaatc acgattgtca
     3181 gtgtctttgt cagtatgctg attctgaaca tcccgctgac gattacaacg ctggttatgg
     3241 tatctatcat gctgggagtg acaaaaaagg tggccggatt ttccggaaat ttctttgtaa
     3301 aacagcagag ggatcttggt aaagtcaacg gctttatcga agagatgatg gatggccaga
     3361 aggttgtcaa ggtattctgt catgaagagg agaatatcga aaaattcaaa gaaattaata
     3421 acaatctgta tgagagtgct tacaatgcca atgcgtttgc gaatgtcatc ggaccgatca
     3481 atgcacagct tggaaatatg agctatgttg tctgtgcgat tgtcggcggt gcacttgccc
     3541 ttggcaatgt cggtggcttt acgctcggtg gtctggcaag ctttctgacc ttcaacaaga
     3601 gttttaacat gccgatcaac caggtcagcc agcagttgaa cgcaatcgtt atggcacttg
     3661 caggagccga acgtattttc cgcctgatgg atgaagagcc ggagctggac gaggggtatg
     3721 taacgcttgt caatgcgaag gaagaaaacg gaaaactcac cgagacgaaa gagcgtaccg
     3781 gaaaatgggc atggaagcat acgcaccagg cagatggatc tgtggattac cgcgagctgc
     3841 agggccgtgt ggtattcgaa catgttgatt tcggctataa cccggataaa atcgttctgc
     3901 acgatgtatc actgtttgcg gagccgggtc agaagatcgc gtttgtcggt tccaccggag
     3961 ccggaaaaac aaccattacc aatctgatca accgttttta tgatattcag aaaggtaaga
     4021 tccgttacga tgagatcaat attggaaaga tcaaaaaaga cgatctgaga agatcgcttg
     4081 gcattgtttt gcaggataca catctgttta cgggaacggt aagagaaaat attgccttcg
     4141 gaaagctcga tgcgacggag gatgaggttg tagcagcagc aaaactggcg aatgcgcacg
     4201 ggttcattat gcgtctgccg cagggctatg acacaatgct gaccggagac ggtgcgaatc
     4261 tgtcccaggg tcagcggcag cttcttgcga tcgcgcgtgc agccattgcg gatccgccgg
     4321 tactgattct cgatgaggcg acaagctcca ttgatacccg taccgagcgc attgttcagg
     4381 atggtatgga taagctgatg agcggaagaa caacctttgt tatcgcacat cgtctgtcta
     4441 ccgtacgaaa cgccgattgt atcatggttc ttgagcaggg acaaattatt gagcgcggta
     4501 cccatgatca gctgatggaa cagaaaggaa aatattatca gttgtatacc ggaaatcagg
     4561 tttctgcata gcagaaaaaa cggaaaaagt aagtcccgca cgacagaaaa taaaaatctg
     4621 tctgtgcggg atttttttgt gaagcagcgg ctggttttat gtgcgagtag cgaagcggat
     4681 tgcgaatatc cgattcgaaa cgatgattgc gagtgataca gtaagttccc acaggttttt
     4741 gggtcttgaa aaagacagcg ggattttata aaatagaaga agcgtttgaa tatatgcaaa
     4801 ggagaaaaac tatggaatac ctgaatacag cgcaggagct gctcgatttt atcatgcggt
     4861 gcaaaagccc gtttcatgtg gtaaaagaag ccggcgatct gctgaacgaa aatggatata
     4921 caaaactgga ggagacaaaa gaatggagtc tcgttccggg cggaaaatat tatgtgacga
     4981 gaaatgaatc ctcggtgatt gcgttccaga tcccggagag cgcattttcc agctaccaga
     5041 tcatcgcaag ccacagcgac tgtccgacgt ttaaagtaaa gggagaggac ccattcgtga
     5101 cggatggtca ttacacccgc ctgaacgtgg agggctacgg cggaatgatc cgttccccat
     5161 ggtttgaccg tccgctttcc ctggcgggcc gcatggtggt acgtgacgga aacagcgtga
     5221 aatccgtgct ggttgatgcc ggacgagatc tggtgacgat cccgaatttg ccgattcatc
     5281 tgaatcgtga catcaacaac ggaatcaaat acagtgtgca gaccgacatg ctcccgattc
     5341 tcggtgatga gacagcgaaa gatcatttct atgagctgtt tctgccggat acggcaaaag
     5401 aggacatcct ctcgatggaa ctttatctgt acaaccgcgt ggagggctct atctggggcg
     5461 ccagcaaaga atttctgtcc agcggccgcc tcgacgatct gcagagcgca ttcggttcct
     5521 taaaaggatt ccttgccgca aaagcgacag gtcaggttaa tgtctgcgca atctttgaca
     5581 acgaggaagt cggaagcctt accaaacagg gcgcagattc gtcgttcctg actgagaccc
     5641 tgcagcacat caacgcagcg tgcggaaaag atacgatcgc ttaccacaga gatcttgcgg
     5701 gaagctttat ggtttctgcc gataatgcac atgcgttcca tccggcgcat gcggagaaat
     5761 acgatccgaa gagccgcgtc tacatgaacg gcggcgttgt catcaaacag agtgcaaacc
     5821 agaaatacac gaccgatgct gtttcggaag ccgtaacaaa gatgatttgc gagaaggccg
     5881 gcgtaccgtg ccaggtattc gcaaaccacg ccgacattcc gggcggaagc acgctcggcg
     5941 caattctgaa ctctcttgtc tcggtgacaa gcgtggatat cggaatggct cagcttgcga
     6001 tgcattcccc gtatgagacg gcaggcgcca aagatactgc ttatctggtg cagttcgcaa
     6061 agaccttcta tgagacgaca ctggtgcgga cagagcatgg attttttctg gaagaaaaat
     6121 aaaagtttaa gatagaaaaa agcggccggc gggacttttg aaaagcctct gccggccgct
     6181 ttttgcgtac ttgcaaagtg tgttttaagg atcagattat tctgtccgga gtgccgtaac
     6241 cggatcgctt tttgccgctt tcttcgacgg aatcagaccg ccgatcaggg tcagaacgac
     6301 gctgagtgca atcagaacaa gcgccgggac gagcggaaga gaagcactga ccttcgtcgt
     6361 atccgccaga tggtggatca gaaggttgcc cggaatcatc agaagcagcg agagcccgat
     6421 gccgatcagt cctgcacaga gtccgatgat gaaggtttcg gcattgaaga cctgcgaaat
     6481 attgcgtttc gaagcaccga tcgcgcgcag gataccgatc tctttcttgc gttccagaac
     6541 actgatatag gtgatgacgc cgatcataat cgaggaaacc acaagggaaa tgccgacaaa
     6601 agcgatcagc acgtagctga tgatgttgac gatatccgtg acggaggaca tcagagtgcc
     6661 gaccatatcg gtgtacgaga ttacctgctc gtttttgcct tctttttcca tcgtgtcatt
     6721 gtaaccgtcg aggatggaga caacgtgttc cttgctctca aagtttttcg ggtaaatgct
     6781 gatcgaagaa ggttcgtcaa aatcagcgta gcccagtttc tgcagattgc cctcataggt
     6841 ggaagagccg gaagaactaa gagaagcgaa gagctctttc atatcatcct cagtcatatt
     6901 catctggaag gcacttgcaa aggcatcggt gtcaatcttc atggcatcgc ccatcgaatc
     6961 ctgaagagaa ttaccgacct gcgtcatggc gcgctccatc tcggactgaa ttgcagaggc
     7021 catctgcgtc atcatctgtt ccatcgcgga agtgatctgt gtctgcagaa ccgaggcgac
     7081 agaagcggag tagcttccca taacgcctgc catgtagttt gtgagcgcag aagaaacctg
     7141 ggactgcagt ccgtcgagat cgacgatttt tgtgataccg ctgttcagcc gctgctggcc
     7201 gtccgccgtg ttcagatagg cgataaagtg ctggcccatc tccgtcggat ccggcaggcc
     7261 gttctccttc acgtaagcga gataaccgtt ggaaagctcc gtcgcaagcg cagtgagctg
     7321 gtcggatgta atttcgatat tgccgttgtt ctgcatcacc tcggaaatcc agtcgttgat
     7381 gatctgaaca gcttcttcgg tctgcagata ctccgcaaga taagcatcaa actgttccgg
     7441 gtcggtgtag cccatcttga tcaggtatgc ctgataccct ttgaagatcc gctgaatgag
     7501 ttcctggatc tgttcctgtg taatggaaac accgccgcct gcaatgatgg tctggatgtg
     7561 gcgccgcagg atttcctgtg cttccgtgga attgatgtag gagacgaaat cctcctgtaa
     7621 atgggaataa tccgcctccg ggtgctccga ggcgtagccc tgatagccct ccatgaggct
     7681 ggaagccagt tcctgcatgc cgtcttcact gacattgaga gtaagcccgg ataaaatctg
     7741 gccaagatca agctgcggca gtccgtcgaa attaagagag atctgactga ggtcaatcgc
     7801 acccgcgaga tccatgctgc ttccggcgtc cgcaaaggca ctggaaaaat caaggttcat
     7861 ggaatcaccg gaaaaagcac cggagagtgc ggaatcgtcg aactggaaag cgttctgcag
     7921 cttgtcctca tcgacagtga taaaggaatc catatcaaga ccgctgttct gatccacggt
     7981 gccgaattcc tgattcgtaa agacatcgat ggaggagtcc gccatctgct gtttcacaat
     8041 ctcggatttc tttgcttcct ccacaacgtg gcgggtcagc gatgccgggt aattgatgcc
     8101 ggcggtcagg gcagaggcgg cagagtcagg ggacggctgg acaataccgg tgattgtgag
     8161 cttttcgccg ttggcaacga gctttttcat atagtcttcg ttgtcgcttt tatctttcca
     8221 gacctgatac tgactgtcgt attcgtagca gtcggcgctg ttcacaagac ggaacgtggt
     8281 gccgaggata tcatcgtagg aatacgtgcc gatggaggaa ggggtctcga cggtttcttc
     8341 atttaaaaac tggcggacca tctcatccag ctcgagtgca tcgcggagcc ccagggtgta
     8401 gagcagaaaa tcactcattc cgccgtcgga agtgaggaca agcacacatt cgttatagtt
     8461 ttccggccag tgtccggctt ttaagtcgta ctggttctcg tacagggaag cggtgtccgg
     8521 catctcgtaa aagacgttgg tgctcatcat ggatgacatg atgctgttgg agcttgcgcc
     8581 aaggccgagc gagcggaacg actggtccgg gtgcacctgg cggatggaat cgccgtcttc
     8641 gcggtagatc tgcggcgtga cggagtagga atattcgatg gcatttgtgt agtcggaaat
     8701 gccgtccgca ccgccgtcaa ggtacttttt cagggaagcg agatcgttgg agttcatggt
     8761 ggagaacatg ttggtcacga cttcctggac tttgatttca ttctttttgc cggaatcttt
     8821 tgagtctttg ccggcattct gtgagccgac catcatcgaa gtgaggtcaa agccggtgct
     8881 ctggatctga agcggatatt cggagagagt ttcctcttcc atattctgaa tatactcgtt
     8941 gacaccagag gagagtgcca gaatcagtgc aatgccgatg atgccgattg agccggcaaa
     9001 cgaggtgagc aaggtccgcg ctttcttggt gcgcaggtta ttaaaactta aggagagcgc
     9061 cgtcagaaag gacatcgacg atttgcccat gtttttgtgt ttgggaggaa gcagagtttc
     9121 ggaatccggc tcaaacgggt tcgtgtcgga gcagatcaca ccgtcgcgca gggtgacgat
     9181 ccggttggcg taaagctcgg cgagctccgg attgtgtgtg accatgacga caaggcgttc
     9241 tttggcaact tcctgcagca ggtccatgac ctgaatactg gtgtcggaat cgagcgcgcc
     9301 ggtcggctca tcggcaagca gaatttccgg atcattgaca agagcgcggg cgatggcgac
     9361 acgctgcatc tgaccgccgg acatctggct cggccgcttg tggagctgtt ttccaagacc
     9421 gaccttttca agtgccgcga ttgctttttt gcgccgcacc tctttggaaa caccggaaat
     9481 ggtaagcgcc agctctacgt ttgcaaggac ggtctggtgc gggatcaggt tgtagctctg
     9541 gaagacaaag ccgatcgtgt ggttccggta ggaatcccag tcgcggtcct tatacttttt
     9601 cgtggaaatg ccgttgatga tcagatcgcc gctgtcgtag cggtcgaggc cgccgatgat
     9661 attcagaagc gtcgtctttc cagaaccgct cggccctaaa atagcgacaa actcgttgtc
     9721 gcgcaaattt aaggacacat cgtcgagcgc tttctgtacc agcgtcccgg tccggtattc
     9781 ttttttgata tgctgaatct gtagcatgaa agggtaactc cttctgtgat taatatgtga
     9841 acagttgtcc tttattctac aacgaaatac gggaaaagtc ccgggcttta gaaaaattta
     9901 agaaaagttt atcgacagaa aaaaagttcc gcctctggtt cggaaaaaca aaaaacggaa
     9961 ctttctttat ctgtgatact tatgatgccg gaaaagtcag acttccatca gaagaccttt
    10021 catataagct tttaagattt tctttttctc cagcgaaaga ttattgtaaa gcgtaatgat
    10081 ttcctgctgt ccttcggtaa gctgcagggc atccggctcg tcgagaaaga aatcggcaag
    10141 tgtgatgccg aaagcgtcgc agagcttctg cagggtaggg atggttggaa tattggtgcg
    10201 gtttaccata ttatttaagg tagaataggg aatgtccgcc tcttttgcaa gacggtaata
    10261 agtccaggaa cgctctttgc ataaatcctg aatgtgctgt atcacatctt tatctctcat
    10321 aaaaagatac ctcgtattcc tatgtaatgt agcggcatga aaattgctac tcacatatat
    10381 tatacgtctg taaaagaata gaaacagatc ttaaagagag atgcagaaaa gctgtacaaa
    10441 taagaaataa tgggaatttg agagaaaaag agagaaaaac ccggcaggaa aataaggtct
    10501 cccgccgggt tctgcaatcg tttcaaagtc tgtgattctt actcttcggt ggaattttct
    10561 tccatctcgt tgaactgctc ggtatccatc agctcgtcga atgcgtctgc tgcaatttcg
    10621 tactcttcat cggaatcgat gttgtcgagg ttcggctggc cgttctcttc gtagtaacga
    10681 tacagataaa cttcgccgtc ttcgttctcg ccgttttcat cgagcggcag aagagcgatg
    10741 tactctttct ggttcggagc tgggaaaatg gtcagtacgg cacattttac ttcggtatcg
    10801 tcgtccagtg tcagggtaac agtcggctga tccagatcct ggcagtcgct gccgcagtct
    10861 gcgcagtctc cgctgcagga tgcgctgttg aataattctt cactcatttg tattaccttc
    10921 ctttttcgtg ttcaagtctt atcaggttct tgtctgccat tctaacagat aatagaaaaa
    10981 aacacaatgg ctgtgccggg aaagcagaaa aaatgcggct gatacaggaa aatacggaaa
    11041 agatacaata aataagaacg aaaaaatgaa aaattagtcg tattttctat tgactgtttt
    11101 cgggtacagt tttatactat cactattgaa tctaaatata caggcagctg aacaaactta
    11161 tcctcaactg ccggggaggc aaaaaatgga tatcagattt gaaaaaagag aacatttaca
    11221 ggaaaaaccg gatcagaaaa atcttggatt cggaaaacac tttacagact atatgtttgt
    11281 catggactgg gacaaagagc agggatggca tgacgcagca atcgtaccgt acggcggaat
    11341 cccgatggat ccggcaacca tggtacttca ctatgcacag gaaactttcg agggtatga
//
LOCUS       DAOMUF010000058        11298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1377_length_11298_cov_3.979365, whole genome shotgun sequence.
ACCESSION   DAOMUF010000058 DAOMUF010000000
VERSION     DAOMUF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1377_length_11298_cov_3.979365"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1398)
                     /locus_tag="PLN77_04910"
     CDS             complement(91..1398)
                     /locus_tag="PLN77_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRM43651.1"
                     /translation="MYKFDREAYDRRMEWYRDARFGMFIHWGLYAIPARGEWVRSTEQ
                     IPKEDYMKYFEEFNPVDFEPRKWAKAAKEAGMKYVVLTAKHHDGFCLFDSQYTDFKST
                     NTKCGRDLVAEYVDAVRAEGLKVGLYFSLLDWFHDDFPHYGDRNHPMRNNPAYKNDDR
                     DFDRYLTYMHNQVREICTNYGKLDVLWFDFSYDTLRGEAWKATELINMVRKLQPDVII
                     DNRLEVSGEGYGSLAAGNPTPYHGDFVSPEQMIPPNGIQDVNGNDIAWESCVTMNNHW
                     GYCANDHFFKPAPMLIKKLVECVSKGGNLLLNVGPDARGNIPEESIERLAEIGKWMKK
                     NGESIYGCGKAGIEKPDFGRVTRHGNHLYFHVYENTVGPLPLPGVKKEEIQAIRYVAT
                     GAEIPVSTSWVHSDYPTMAFADLGEDPVLPDGVDTVIDVILKK"
     gene            complement(1516..2886)
                     /locus_tag="PLN77_04915"
     CDS             complement(1516..2886)
                     /locus_tag="PLN77_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM43652.1"
                     /translation="MGYLGNKLQGKYHKDTFQTAVNMAWPAIVESFFIAFAGLVDSLM
                     VSSLGSYAVAAVGLTTQPKLLGLAFFFALNVSISALVARRRGEQRQGAANETLVTALV
                     FIILAAAAFSVGFVAFASPIIHLCGSTAETHNGAVTYFRIIMGGMIFNCIQMGINSAQ
                     RGAGNTKITMRTNVTSNTINIILNYLLINGHFGFPALGIQGAALATVTGTVVGCVMSI
                     LSITKQDGFLNLGYILKNKIKPTIAAFISLVRVGYSVFFEQVFMRIGFMMTAIMAAKQ
                     GTDAMAAHQVGMNIMSLSFAFGDGLQSAAVALIGRSLGAKKPDLAKEYGRTCRLIGAG
                     IAVCLVAIYLFGGRWLYSLFFEEQHIIEIGVSIIHVIIFVVIFQICQVIYMGCLRGAG
                     DTLYTAVASIISVTFIRTIVSYLGGYTLGLGIVGIWFGVLADQMSRFIFATIRFKQGK
                     WVKIKI"
     gene            complement(3087..5306)
                     /gene="bglX"
                     /locus_tag="PLN77_04920"
     CDS             complement(3087..5306)
                     /gene="bglX"
                     /locus_tag="PLN77_04920"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007889148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HRM43653.1"
                     /translation="MTEQKLKELLADMTLEEKVNQMSQVVGAFFNKDMDITAMGPMAD
                     KGFTPENVALSGSILGSMGAETLKKIQKDFVEQHPHHIPMLFMLDVINGFKTIFPIPL
                     GQGATFEPELSEKCAAVAAKEAAVSGLHVTFAPMTDLVRDARWGRVMESTGEDPYLNG
                     LFCAGMVRGFQGDDLKEPYKIASCVKHFAGYGAPTAGRDYNTVELSEHTFRDFYLPSY
                     KAGIDAGAAMVMTSFNTVNGVPATGNKKLMRGILRDEMGFDGVLISDWAAIEEIIYHG
                     YCADREEAAVRSAEAGVDIDMMTGIYCENLCRLVREGKISEDLIDESCMRILELKNKL
                     GLFENPYKDADETKEKEVILCKEHRDLAREAARKSFVLLKNEEKILPLGKEKKIAWVG
                     PYVHSRNLMGSWSFIGDAKDVTNLEEAVKAQADTTNMSFHAGSPMLGSDIRLEGFGEA
                     MEQSTTPEEEEAMLLEAVNAAKEADVVVLAIGEDRLQSGEATSNANIRIPEIQQRLLE
                     RVSKVNENVVVVLFNGRPLDLRDVVSKAKAVLEVWMPGTEGANAIADVVFGAYAPSGK
                     LTMSFPYSVGQVPVHYNEYSTGRPHVPGKDKDRFRSKYLDIPNAPLFPFGYGLGYTSF
                     AISDVKLDKAELGMEDEIKASVTVKNTGDTKGTETVQLYIHDVAASVVRPVKELKDFC
                     KVTLQPGEETEVIFKITEEELRFLTENERVESENGVFEVFIGSDSTTENKAEFVLKK"
     gene            complement(5553..7148)
                     /locus_tag="PLN77_04925"
     CDS             complement(5553..7148)
                     /locus_tag="PLN77_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM43654.1"
                     /translation="MKLLLVDDEEYVIESIKRNLDLTETGVSEVYTAFSVQQAKNIMG
                     MVDIDIVISDIVMPGGTGFDFVEWVRKEELKVQVIFLTSYAEFDYARRAIQLNSVDYL
                     LKPIDFDKLKEAVQRAAESVAKEKKIQDLRQESIKWNQNRKILQQDIWKNVLKDGFSE
                     EKFCETAAQRQLPYGPGHYFRLICLMPEIKSNKREMWDTATMEFVIENVLSELYEETD
                     IAVDTVFYQEPGSYWIVTQSKEKAFPQDGERDCEILRQFVAWMNEKIYLELWCGAGQW
                     ESVLGMQKQSRNLQKMREGSLSVRNEVLFLNEFQAPQTSYENKELDVWKALLSEEKPE
                     ELLERLQKYLDRTEREGMITRELLVALRTDLTQVVYAWLSEREIKAYALFADADMENL
                     HRNSLYGREEMMEYAGVLVRKAVQYRQYINKSDSVTSQVCAYIDAHYREEIHREELGE
                     LVFLNTDYLSRIFKKEKGISISNYIIQKRVEEAKKLLTQSTLPINTVSLYVGYSNFSY
                     FTKMFKDNTGYAPLEYRRSFSEK"
     gene            complement(7126..8868)
                     /locus_tag="PLN77_04930"
     CDS             complement(7126..8868)
                     /locus_tag="PLN77_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM43655.1"
                     /translation="MRISKQTKKTTLAQQGRYLLICLLSMLLLFLAGSVLILNRTQRQ
                     VYEQLEEISKLYTDELDNRFFRISRNLFSTVMDSSNPDSAFWKYMDLMEKDQYEEYVI
                     TQLRRNYASAAWDFGTDYNVFLYTQKDDSLYQLSISSDGLYAVDPYLQEALKRRIKSL
                     SQQTYAVKKKWTVMCQGDDVYMLKVAQSQGVGLGCYVNLKTILEPFSELSLGKSGYVS
                     LVDQNGKSIGTLTEKGIVTDSSKFDTGNYTFRQELSQAPFEIRIKISWTGVLNLMTGS
                     VFALLGVAFLMVIAGSVLLFHLETKILKPVGMFMENLQKYDNGDLTYQMSEGNLVELE
                     QIDDKFRNMIHQIRRLKITLYEQELQKQKIEMDYLKIQIRPHFYMNCLNFIYSMIDFG
                     QYDHAKSMSRITSDYLAYIFRNTSEMVPVTAETNHCENYLKILLLRYPEKFTYYFEVH
                     EEVENAVIFPFLIQVFVENAAKHALTLETVPLISVTVYPEDRGDEKYVNIYISDTGNG
                     FPEETLQRLKAGEDISEHGKHIGIENCLKRFRYYYGDSGEIHFDNSPLGGAIVDIHIP
                     YRTGGEEHETIARG"
     gene            complement(9060..9962)
                     /locus_tag="PLN77_04935"
     CDS             complement(9060..9962)
                     /locus_tag="PLN77_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM43656.1"
                     /translation="MTNSKKAKRFQFWINVVLILITLSIVLPFVLVFISSLTDENTLI
                     RNGYSFFPEKFSFYAYSYIIRQGEKILRAYGITVFVTIVGTVANLAMSALLAYPLSIK
                     KLPYRRAITFFVFFTMLFNGGLVPTYLMYVNYLHIKNTIWALIVPGFLLSANNVLMIR
                     SYFNTSIPDALFEAAKIDGAGHMKIFTSITLPLGKPILVTMGLFSALGYWNDWTNGLY
                     YLSGNEGQKLYSIQNLLNQMITDIQYLASGKVTGNIGAEVAKLPTTSIRMAIAFIAML
                     PLFIIYPFLQKYFAEGITLGAVKG"
     gene            complement(9975..10913)
                     /locus_tag="PLN77_04940"
     CDS             complement(9975..10913)
                     /locus_tag="PLN77_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM43657.1"
                     /translation="MKKAEVKKEKKKNVLKEYWPLYLMMLPALLYLLINNYIPMAGMV
                     IAFKKLNFAKGIWASPWAGLDNFKFLFSSKDAWIITRNTLLYNVAFILINMVVGIAIA
                     ILITEVRNTKLKKVYQSAILLPFLMSMVILSYIVYALLSAENGLVNNSILPFFHIDPI
                     QWYQKPKYWPAILIIANCWKGVGYGCLIYIASLIGIDPSFYEAARLDGASKWQEITKI
                     TLPSLVPTIITLLLLSIGRIFYSDFGLFYQVPMNSGVLFPTTNVIDTYVYRALIEQGN
                     ISMSSAAGVYQSLVGFCVVMLSNWIVRRVDKDQALF"
     gene            complement(11064..>11298)
                     /locus_tag="PLN77_04945"
     CDS             complement(11064..>11298)
                     /locus_tag="PLN77_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HRM43658.1"
                     /translation="ASGIESKALGFVFDNSEYADQVSALNNVISQYNGALSSGSGDPE
                     ELLPQFLEALDDAGIDDVIAAKQEQLDAFLAAK"
BASE COUNT         2655 a         3030 c         2501 g         3112 t
ORIGIN      
        1 acgtggcagg aaacttcagc gaactttcct gccacgcaaa aaatcagcca cagaaaccct
       61 ttcggaaaac tgtggctgat gctttaccct ctactttttc agaatcacat cgatgactgt
      121 atcgactcca tccggcaata ccgggtcttc gccgagatcg gcgaatgcca tggtcggata
      181 atcgctgtgt acccagcttg tggaaaccgg gatttccgcg ccggttgcga cgtagcggat
      241 cgcctgaatt tcttcttttt tcacgccggg cagcggcagc ggcccaacgg tattttcata
      301 gacatgaaaa tacagatggt ttccgtggcg cgtcacacgg ccaaagtccg gtttctcaat
      361 accggctttt ccgcagccgt aaatgctctc tccgtttttc ttcatccact ttccaatctc
      421 agccagccgt tcgatcgact cctccgggat atttcccctg gcgtccgggc cgacgttcaa
      481 aagcaggttt ccacccttcg atacgcactc gaccagcttt ttgatcagca tcggtgccgg
      541 tttgaagaag tggtcgttgg cacaataacc ccagtgattg ttcatcgtaa cgcaggattc
      601 ccaggcgata tcgtttccat ttacatcctg gatgccattc ggcggaatca tctgctccgg
      661 gctcacaaag tcgccgtggt acggggtcgg atttccggcc gccagagaac cgtagccctc
      721 tccgctcacc tcgagacggt tgtcgatgat gacatccggc tggagcttcc gcaccatatt
      781 gatcagctcc gtcgctttcc atgcttcgcc gcgcagcgta tcgtaggaaa agtcaaacca
      841 cagcacatcg agctttccgt agtttgtgca gatctcgcgt acctgattgt gcatgtaggt
      901 caggtagcgg tcgaaatcgc ggtcatcgtt tttatatgcc gggttgttcc gcatcgggtg
      961 attccggtcg ccatagtgcg gaaaatcgtc gtggaaccag tcgagcaggg agaaatacaa
     1021 accgaccttc aatccttctg cgcggacggc atcaacgtac tctgcaacga ggtcgcggcc
     1081 gcatttcgtg ttggtggatt tgaaatcggt atactggctg tcgaagaggc agaatccatc
     1141 gtgatgcttg gcagtcagaa ccacgtattt cataccggcc tctttcgccg ccttggccca
     1201 tttccgcggc tcgaaatcaa caggattgaa ctcctcaaaa tatttcatgt aatcttcttt
     1261 tggaatctgc tcggtactcc gcacccattc tccgcgcgcc gggatggcat aaagccccca
     1321 gtggataaac atgccgaaac gggcgtcgcg gtaccactcc atccgcctgt cataggcttc
     1381 tctgtcaaat ttgtacatcg ttttgcttcc tcctgtttta gttttatatt ggtaatgatt
     1441 cagagtacca tttcgcatgg ctctgaatcg ttacgtcaag cggatattcg caatccgctt
     1501 cgctgcttga ttcgattaaa tcttaatttt cacccatttt ccctgtttga atcggatcgt
     1561 tgcaaagata aaccgtgaca tctgatccgc cagcacgccg aaccagattc cgacgatgcc
     1621 aagccccagc gtgtaaccgc cgagatacga aacgatcgta cgaataaatg taacgctgat
     1681 tatagatgca actgccgtat aaagcgtatc tcccgcaccg cgcaggcatc ccatataaat
     1741 aacctgacag atctggaaaa tgacgacgaa gataatgaca tggatgatgc tgacaccgat
     1801 ttcaatgata tgctgttctt cgaaaaacag gctgtacagc catcttccgc cgaaaagata
     1861 aatggcaacg aggcatactg cgatgcccgc gccgatcaga cggcacgttc tgccgtactc
     1921 cttcgccaga tccggctttt ttgcgccaag gctccggccg atcagtgcaa ccgctgcgga
     1981 ctggaggcca tcgccgaacg caaacgaaag cgacataata ttcatgccga cctgatgtgc
     2041 cgccatcgca tcggttccct gcttggccgc catgatcgcc gtcatcataa agccgatccg
     2101 catgaaaacc tgttcgaaaa acacactgta cccgaccctg accaggctga tgaacgctgc
     2161 aatcgtcggt ttaattttgt tcttcagaat atagccaagg ttcaggaatc catcttgctt
     2221 tgtgatggaa aggatgctca tcacacatcc gacaaccgtt cctgtgacgg ttgccagtgc
     2281 cgcaccctga atgccgagtg ccgggaaccc gaaatggccg ttgatcagaa gataattcag
     2341 gataatatta atcgtatttg atgttacatt tgtgcgcatc gtgatttttg tattgcccgc
     2401 accgcgctgt gccgagttga tccccatctg gatacagtta aaaatcatac cgcccatgat
     2461 gatacggaaa tatgttactg cgccattatg agtctccgcc gttgaaccgc acagatggat
     2521 aatcggactt gcaaacgcca caaatccaac actgaatgca gctgccgcaa ggataataaa
     2581 tacaagcgca gttaccagag tctcattcgc cgcaccctgc ctctgctctc cgcgccgccg
     2641 cgcaacgaga gcagaaatcg acacattcag cgcaaagaaa aacgccaggc caagcagctt
     2701 cggctgtgtc gtcagaccga ccgcggcaac tgcgtaggag ccaagcgagc ttaccatcag
     2761 cgaatctaca agacccgcaa atgcaatgaa aaacgactcc acgatcgccg gccacgccat
     2821 gttgacggcg gtctggaatg tgtctttgtg gtatttcccc tgtaacttgt ttcctaagta
     2881 ccccatttct tttcctctct ttcgtcccaa taccaatttc tattttactt tgttttaaca
     2941 gaagggtcaa tacggtaccg gcagaaaaaa gccagccgca aaagcaaaat tcttttcgct
     3001 ttttgtggct ggcttttctt ttatctttcc gtagcgattc agacccccat cttgattccg
     3061 cttacgctac atcccagtat tgcttcttat tttttcagca caaattctgc cttattctct
     3121 gtcgtgctgt cgcttccgat gaagacttcg aaaacaccgt tttcactttc tacacgctca
     3181 ttttctgtca ggaagcgaag ctcttcttct gtgattttga agatgacttc ggtttcttct
     3241 cccggctgaa gggttacctt gcagaaatct ttcagctctt tcaccgggcg taccacggat
     3301 gcggctacgt cgtggatgta aagctgtacg gtctcggttc cctttgtatc gccggtgttt
     3361 ttcacggtta cggaggcttt aatctcatct tccatgccaa gctctgcctt gtccagtttc
     3421 acatctgaaa ttgcaaagct ggtgtagcca aggccatatc cgaacgggaa cagtggtgcg
     3481 ttcgggatgt cgaggtattt ggaacggaaa cggtctttgt ccttgcccgg cacatgcggt
     3541 cttccggtgg aatactcgtt gtagtgaacc ggcacctggc ctacagagta cgggaagctc
     3601 atggtcagct tgccgcttgg agcgtatgca ccgaaaacaa catctgcgat tgcattggca
     3661 ccctcggtac ccggcatcca cacttcaaga acagcttttg ctttcgatac gacatcgcgc
     3721 agatcgagcg gacggccatt gaagaggaca acgacaacgt tttcattgac ttttgaaacg
     3781 cgctcaagca gtctctgctg gatttccggg atacggatgt tcgcgttgct ggttgcctcg
     3841 ccggactgca gacgatcctc accgatcgca agaacgacaa catcggcctc ttttgctgcg
     3901 tttacagcct caagaagcat tgcttcttcc tcttccggcg tggtggactg ctccatcgcc
     3961 tcgccgaagc cctccaggcg gatgtcggag ccaagcatcg gagagcctgc atggaagctc
     4021 atgtttgtcg tatctgcctg cgcttttacg gcctcttcca gatttgtcac atcctttgca
     4081 tctccgatga aggaccagga gcccatcagg tttctgctgt ggacatacgg tccgacccag
     4141 gcaatcttct tctccttgcc aagcggcagg attttctctt cgtttttcag cagtacaaac
     4201 gattttcttg cggcttctct tgccagatcg cggtgctctt tgcagagaat gacctctttt
     4261 tctttcgtct cgtctgcgtc tttatacggg ttttcaaaca gtcccagttt gtttttcagt
     4321 tccaggatac gcatgcagga ctcatcgatc agatcctcgg aaatcttgcc ctctcttaca
     4381 aggcggcaga ggttctcgca gtaaatacct gtcatcatat cgatatcgac gcctgcctcg
     4441 gcggagcgga cagctgcctc ctcacggtct gcgcagtagc cgtggtaaat aatctcctcg
     4501 attgccgccc agtcggaaat cagaactccg tcaaatccca tttcgtcacg caggatgccg
     4561 cgcatcagtt tcttgtttcc ggttgccgga acaccgttta ccgtgttgaa ggaagtcatt
     4621 accatcgctg cgcctgcatc gataccggct ttgtaggatg gcagatagaa gtcacggaag
     4681 gtatgctcgg aaagctctac ggtgttgtag tctcttcccg ctgtcggagc gccatagcct
     4741 gcaaaatgtt tcacgcagga agcaattttg tacggctcct tcagatcgtc tccctggaat
     4801 ccgcgcacca ttccggcaca gaacagaccg ttcagataag gatcctcacc ggtagactcc
     4861 atgacacggc cccatcttgc atcgcggacc aggtcggtca tcggtgcaaa ggttacgtga
     4921 aggccggaaa cggctgcctc ttttgcagca actgccgcac acttttcgga gagctccggt
     4981 tcaaacgttg cgccctgtcc cagcggaatc gggaaaatgg tcttgaagcc gttgatgaca
     5041 tccagcatga acagcatcgg gatgtgatgc gggtgctgct ccacaaaatc cttctggatt
     5101 ttctttaacg tttctgcgcc catgctgccc aggatggaac cggaaagcgc tacattttcc
     5161 ggcgtaaagc ctttatccgc catcggtccc atggctgtga tgtccatatc tttattgaag
     5221 aaggcaccca ccacctggct catctggttc actttttctt ccagcgtcat gtcggccaga
     5281 agctctttta atttctgctc ggtcattttt tctcctccat acctcgtttt tgctgtatct
     5341 gcttcttcag aaacccctaa aatgtcagaa aaatatcgtt tttccgtttt ttctgaatct
     5401 tatactgaaa ttttaccaca tctgacctga aaaaaatatc ccctgatccg acctttttct
     5461 ttcactattc cgacctgttt gctgctattc cggtgcgtaa gaaagtgata aaatcctctg
     5521 ttatttcccg gacatgtgaa gggggctttc cgctattttt cactaaagct tcttcggtat
     5581 tccagcggtg cgtatcccgt attgtcctta aacatcttcg taaagtacga gaaattgctg
     5641 taccccacat acagcgacac cgtattgatc ggcagcgtgc tctgtgtcag aagctttttt
     5701 gcctcctcca cccgcttttg gatgatataa ttactgatcg aaataccctt ttctttttta
     5761 aaaatccggg acagataatc cgtattcaaa aagacaagct ccccaagctc ctcccggtga
     5821 atctcctccc ggtaatgtgc atcgatataa gcgcatacct gactcgtgac cgaatctgat
     5881 ttgttgatgt actgtctgta ctgaactgct ttccgaacca gtaccccggc atattccatc
     5941 atctcttctc tgccataaag ggaattccga tgcaggtttt ccatatccgc atctgcaaac
     6001 agtgcatacg ccttgatctc ccgttccgaa agccatgcat acaccacctg cgtcagatcg
     6061 gtacggagtg ctaccagaag ctccctggta atcattcctt cccgctccgt ccggtcaagg
     6121 tatttctgca gccgctccag cagttcctcc ggtttttcct ccgaaagcag agctttccac
     6181 acatccagct ccttattttc gtagctggtc tgcggagcct gaaattcgtt cagaaacagc
     6241 acctcgttcc gcacggaaag gcttccctcc cgcattttct gaagattccg gctctgcttt
     6301 tgcatcccca gtacagattc ccactgtccg gcaccgcacc acagctccag atagattttt
     6361 tcattcatcc acgcaacaaa ctgccgcaga atttcacaat cccgctcacc gtcctgcggg
     6421 aatgcttttt cctttgactg tgtaacaatc cagtagctgc ccggttcctg gtaaaaaaca
     6481 gtatctaccg caatatccgt ttcctcatac agttccgaca gcacattttc gatcacaaat
     6541 tccatggtcg cggtatccca catctcccgc ttattgctct ttatttccgg catcagacaa
     6601 atcagccgga aatagtgtcc cggtccgtat ggcagctggc gctgtgctgc cgtttcgcag
     6661 aatttttcct cggagaagcc gtctttcagc acatttttcc agatatcctg ctgcagaatt
     6721 tttctgttct ggttccactt gatgctttcc tgcctgagat cctgtatttt cttttctttc
     6781 gcaacggatt ccgccgcacg ctgtacggcc tccttcagct tgtcaaaatc gatgggcttt
     6841 agcagataat cgacactgtt cagctgaatc gctcttctgg catagtcaaa ctccgcatag
     6901 cttgtcagga atatgacctg gaccttcagc tcctcttttc tgacccactc cacaaagtcg
     6961 aatcccgttc cgcccggcat cacgatatcg ctgatcacga tatcaatgtc caccattccc
     7021 atgatgttct tcgcctgctg tacggaaaac gcagtgtaca cctcgctcac cccggtctct
     7081 gtaagatcga ggtttctttt gatactttca atcacgtact cttcatcatc cacgagcaat
     7141 agtttcatgt tcttctcccc ctgtcctgta tggaatatgg atatctacaa tcgctccgcc
     7201 gagcggactg ttatcgaaat gaatctctcc ggaatcgcca taataatagc ggaatcgttt
     7261 caggcagttt tcaatcccga tatgctttcc atgctcggaa atatcttccc ctgctttcag
     7321 cctttgcagc gtctcctccg gaaagccgtt tccggtgtcc gaaatataaa tgttgacata
     7381 cttttcgtct ccgcggtcct ccgggtaaac cgtcacggaa atcagcggaa ccgtctccag
     7441 cgtcagggca tgttttgccg cattctctac aaacacctga atcaggaacg ggaagattac
     7501 tgcgttttcc acctcttcat gaacctcaaa ataataggtg aatttttccg gatagcgcag
     7561 cagcagaatt ttgaggtaat tttcacagtg gttcgtctcc gccgttaccg gcaccatctc
     7621 gcttgtattc cggaaaatat acgcaagata atcggacgta atacggctca tcgactttgc
     7681 atggtcgtac tgaccgaagt cgatcatgct gtagataaag ttcagacagt tcatataaaa
     7741 atgcgggcgg atctggattt tcaggtagtc catctcgatt ttctgttttt gcagctcctg
     7801 ctcatacagc gtgatcttca accggcggat ctggtgaatc atgtttcgga atttatcgtc
     7861 aatctgctcc agctctacca ggtttccttc cgacatctga taggtgaggt ccccattgtc
     7921 atatttctga agattttcca taaacatacc gaccggcttc aggatttttg tttccaggtg
     7981 gaacaacagc acggaaccgg caatcaccat cagaaaagca acgccaagaa gggcaaacac
     8041 acttcccgtc atcagattta agactcccgt ccaggagatt ttaatccgga tttcaaacgg
     8101 tgcctgcgaa agctcctgcc ggaaggtata attgccggta tcaaacttcg aactgtccgt
     8161 tacaattcct ttttccgtca gtgttccgat gctttttccg ttctggtcaa cgaggctgac
     8221 atagccgctc ttcccaagag acagctcaga gaacggttcc agaatcgttt tcagattgac
     8281 gtagcagccg agtccgacgc cctgactctg cgctaccttg agcatataaa catcgtctcc
     8341 ctggcacatc acggtccatt ttttcttgac tgcatacgtc tgctgcgaca ggcttttgat
     8401 cctgcgcttc agcgcttcct gcaaataggg atcaacggcg taaagcccgt cggaggaaat
     8461 gctcagctga tataagctgt catccttctg cgtatagaga aaaacattgt aatccgttcc
     8521 aaaatcccac gctgcagatg cataattcct tcgcagctgc gtgatgacat attcctcata
     8581 ctgatccttc tccatcagat ccatatattt ccagaaagca gaatccgggt tggagctgtc
     8641 cattacagtc gagaacaggt ttcggctgat tcgaaaaaaa cggttatcca gctcatccgt
     8701 atacagcttt gaaatctctt ccagctgctc atatacctgc cgctgcgtcc ggttcagaat
     8761 aagcacgctc ccagccagaa aaagcagcag catactcagc aggcagatca aaagataacg
     8821 cccctgctgc gccagtgttg tttttttcgt ctgttttgaa atacgcatga agtttcctcc
     8881 ttttttctgt tccggtacgc tcgaaatttt tccgtgtttg attttacgaa ctgatctttt
     8941 tttctgaata taatacagca gaaaaacagt acacctcatg tttccatgag tctgtactgt
     9001 ttttctgatt tatgtcggaa acaccgaaat gtttctcttt tttattcctg catggaaaat
     9061 catcccttta ccgcaccaag tgtgatacct tctgcgaagt atttctgaag gaacggatag
     9121 atgataaaca gcggcagcat ggcgataaat gcgattgcca tacggatcga ggttgtcggc
     9181 agctttgcca cctcggcacc gatatttccg gttacttttc cacttgccag gtactgaata
     9241 tcggtgatca tctggttcag caggttctgg atactgtaaa gcttctggcc ttcatttcct
     9301 gataaatagt ataaaccatt tgtccagtca ttccaatagc caagtgcgga aaacagtccc
     9361 atggttacca gaatcggttt tccaagcggc agtgtgatgc tggtgaaaat cttcatgtgt
     9421 cctgcgccat cgattttggc cgcctcgaac agcgcgtccg ggatactggt gttaaagtag
     9481 cttcggatca tcagcacgtt gtttgcactt aacaggaagc ccggaacaat caatgcccag
     9541 atcgtgtttt tgatgtgaag ataatttaca tacatcaagt aggtcggcac cagtccgccg
     9601 ttgaacagca tcgtgaagaa tacgaaaaag gtaattgctc tgcggtacgg cagcttttta
     9661 atcgaaagcg gatatgccaa cagagcggac atcgcaaggt ttgcaactgt accaacgatg
     9721 gttacaaaaa cggtgattcc gtacgctctc aggattttct caccctgacg gatgatatag
     9781 gagtaagcat agaagctgaa tttctccggg aagaacgaat atccgttgcg gatcagtgtg
     9841 ttttcatcgg tcagcgagga aataaatacg agtacaaacg gcagtacaat ggataacgta
     9901 atcagaatga gtactacatt aatccagaac tggaaacgtt ttgctttttt tgaatttgtc
     9961 atacttccat cctcctagaa taatgcctgg tctttgtcta ctctgcggac aatccagtta
    10021 ctcaacatta cgacacagaa gcctaccaga gactggtata caccggctgc agatgacata
    10081 ctgatatttc cctgctcaat cagggcgcgg tatacatagg tatcgataac gtttgtggtc
    10141 gggaacagaa caccggagtt cattggtacc tgatagaaca gaccgaaatc ggaatagaaa
    10201 attcttccga tgctgagaag cagcagggta atgatggtcg gaacgaggct cggcagtgtg
    10261 atttttgtga tctcctgcca tttgcttgcg ccgtccagtc tggcagcctc ataaaaggaa
    10321 ggatcgatac cgatcagcga tgcgatgtag atcaggcatc cgtatcctac gcccttccag
    10381 cagttcgcga taatcaggat cgccggccag tatttcggtt tctggtacca ctggatcggg
    10441 tcaatatgga agaacggaag gatcgaattg ttgacaagac cattttccgc actcagaagc
    10501 gcatatacga tgtagcttaa gataaccatg gacatcagga acggaagaag aattgcactc
    10561 tggtacactt ttttgagctt tgtgtttctt acctccgtaa tcagaatcgc aatggcaatt
    10621 ccgactacca tgtttataag aataaatgca acgttataca gcagcgtgtt tcttgtaatg
    10681 atccatgcat ccttcgagga aaacaggaac ttaaagttgt cgagtcctgc ccatgggctt
    10741 gcccagatac cttttgcaaa gttcagtttt ttaaacgcaa tcaccatacc tgccatcggg
    10801 atatagttgt tgatcagcag atacagaagt gccggaagca tcatcagata aagcggccag
    10861 tattccttca gcacgttttt ctttttctct tttttaacct ctgctttttt cataaagcgt
    10921 gtctcctttt atgagggctg ttttttcagg ttctttcaca gagaaagctc cacatctttt
    10981 cgggtaagag tggagccttc ggttactgta tcacaccgga ttttcttttg ccccggtcgt
    11041 gttcttttta caaattatgg tgcttatttc gctgcaagaa atgcatccag ctgctcctgc
    11101 tttgcagcaa tgacatcatc gattcccgca tcgtccagtg cctccaggaa ctgtggaagc
    11161 agttcttccg gatctccgga gccgctgcta agtgcgccgt tgtactggct gattacgttg
    11221 ttcagggctg atacctgatc cgcatattcg ctgttatcaa agacaaatcc cagtgctttc
    11281 gactcgatac cggaagcg
//
LOCUS       DAOMUF010000059        11148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1406_length_11148_cov_4.465248, whole genome shotgun sequence.
ACCESSION   DAOMUF010000059 DAOMUF010000000
VERSION     DAOMUF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11148
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1406_length_11148_cov_4.465248"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..830)
                     /locus_tag="PLN77_04950"
     CDS             complement(93..830)
                     /locus_tag="PLN77_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43659.1"
                     /translation="MEASLVLPVFLIAVLVLLKIVDLYRLHGMLTVSLQESARYLSVC
                     AYAVDETGKETYEIPETAACIAYAEAKIPKEIRENGMVTLAGSRVKNGWLELKAIYWP
                     KMGNRLVPVRNRGIAACAKVHLFCGKTEEEADEEEGEAKQMVWVTENGSVYHTDSSCS
                     HINLSIQVSSPGALAWKRNQDGEKYKECEQCVGNGAAGAEVYYTKYGNRYHNSLSCKG
                     LKRKVKLVALEQTDGLPQCSKCAKKAA"
     gene            complement(997..2529)
                     /locus_tag="PLN77_04955"
     CDS             complement(997..2529)
                     /locus_tag="PLN77_04955"
                     /inference="COORDINATES: protein motif:HMM:NF039199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HRM43660.1"
                     /translation="MRETERQKRWAAGSITAYLCLVLILMLSLISGTIASVRNAHARV
                     MTACAMEQGLYSLFAGYDRTLLGQYELFFYNGGDKSGVWKPETMSRKVAETAGEVLSP
                     TASGMGILSGSLSGGQVTEVYLGGYRLATDEEGSAFSRQICEAMKASLGTYGIQQLSQ
                     KLRGEQETVKRQEDGQSGYEEEEVLKQYEESKQPEEGTEKTEGEGESGNGTPSEPDQK
                     QPVPEDFVNPIEVIQKVMKMGILGLVLPEGQALSQGETDVSFLASKRELQSGFGMAEC
                     GKELSFVDKMISSEYILWKFPCYTSKEKKEGLQCQVEYVLCGKNSDQDNLEGTVNRLL
                     ALREAANLVYLAKDAQKQGEMEAMAGVIAASFGVPAAMPVVKMALAVCWAFAESILDL
                     RELLDGGKVALFKTAESWQLSLEQLPKLLETGDQSRKNAPGGMEYSDYLRLLLMQKSG
                     KAVTFGAMDLVEYNMRTEQQQPGFRLDCCVDELEAGFTAVIGKREYEITRNYGYEMQG
                     AA"
     gene            complement(2686..2853)
                     /locus_tag="PLN77_04960"
     CDS             complement(2686..2853)
                     /locus_tag="PLN77_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HRM43661.1"
                     /translation="MKLISEFWKEEDAVGVVEVILILVVLIGLVLIFKKQLVSLVNSL
                     MEKVTNQSNTV"
     gene            complement(2904..4202)
                     /locus_tag="PLN77_04965"
     CDS             complement(2904..4202)
                     /locus_tag="PLN77_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRM43662.1"
                     /translation="MLWICLTAWAGLFIWYFRMRKKAEDHKLVRRIFCLAATGFIAGT
                     ALCLPIGVRKVSGDEKAELQLERGALGSQPTEEKLEVSVGNQKPERITLSVPARAYSA
                     EEIEEAFSAAIEALDEIILGENLSFHTVNRNMDLVTEIPGSPVALSWETDRPLLIDSR
                     GLLSDEIPEEGVEVTLKAELELQGETTEYIRKVQVYPPEIKGRDAVLRALKKAVEKEN
                     EAHPEETAYYLPETLNGETVTWSKVPDLTGLELMALAAAAGAVCWAAKGREQEKARQK
                     REEQMLRDYPEIVSKMVLLLGAGLGMRKVLERIAVDYRKNLALGGQKRFAYEEIVFTC
                     QEMENGVSEQEAYQRMGMRMGTGAYRSLAVLLTQNLKKGSKGLLELLKQESQEAFEER
                     RRQAKTTGEKASTKLLLPMGMMLAVVLVILTVPAFLSFYA"
     gene            complement(4274..5074)
                     /locus_tag="PLN77_04970"
     CDS             complement(4274..5074)
                     /locus_tag="PLN77_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRM43663.1"
                     /translation="MRIRAGSQAKTGMAEEKETKGIDYQTWEFTGREYLEIAGISLGI
                     AAAVNLLCYRAWWACIAVVPVGIVCFHTYRKNRIQRRKEELYDSFRDLIAWMHTALRS
                     GYSMENAVLEAAGQLEQSLGKENILVQELRRMRHKMMISVPVEQLFQDLAVRSRIDDI
                     ATFASVLVIAKRTGGNVCEIFQNTWDIFCTRIDTMREIRAGVSSRRFEQNIMSVVPFG
                     ILGYVQLSFPEFLSVMYGNVIGVFFMSGCLAVYLAAWVLGKKILDIEF"
     gene            complement(5071..6249)
                     /locus_tag="PLN77_04975"
     CDS             complement(5071..6249)
                     /locus_tag="PLN77_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRM43664.1"
                     /translation="MTMPEILRRKLMERIDIASEPDDGEVLEEIDGLILGEEETRYLP
                     VAQKAELRRQLFYSVRRLDVLQELLEDAGITEIMVNGYQHIFVERNGRIERYGKSFTS
                     AEKLEDVIQQIVGRCNRVVNEQNPIVDARLENGDRVNVVMKPVALNGPILTIRRFPEQ
                     AVTMEFLVSIGSITKEAAEFLQALVRTRYSMMIGGATGSGKTTFLNALSAYIPKGERI
                     ITIEDNAELQIQGVENLVRLEAKEANLESGTEITIRDLIRAALRMRPNRVIIGEVRGA
                     ETFELLSALNTGHAGSLSTAHANSVKDMISRLETMVLMGVQLPLEAIRRQIASGIEIL
                     VHLGRGEDGSRRVEEIAEITGMEEDEIKTVSLFRREYGRGLQKTGELVHREKLEGKTG
                     "
     gene            complement(6246..7283)
                     /locus_tag="PLN77_04980"
     CDS             complement(6246..7283)
                     /locus_tag="PLN77_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43665.1"
                     /translation="MNTAKKKSVFAVCDTEMEYARNFMEYLNQKRNIPFEVHAFSSAE
                     MFLEFAKNNETEILLISDKAMKEEIRRYPIRQIIILSEGVHSPVLDQYPAVYKYQSSE
                     KVIREVMACYGVENGVDTSPALLPKKEMRIIGIYSPVGRTQKTSFALTMGQILAKERA
                     VLYLNMESYSGFERLLECSYDRGMSDILYYARQENQGIIYKLGGMVQSMQNLDYLPPA
                     ASPMDIQTAKYEEWKWLFQEIEKNSSYEVLILDLGDGVADLYQILDFCNEIYVPIRND
                     VISAAKMEQFENLLRRWDCQSVLDKMRKIHVPFHTANRTGKAYFEELVWSELGDYVRQ
                     ILRDGRRGEET"
     gene            complement(7280..7819)
                     /locus_tag="PLN77_04985"
     CDS             complement(7280..7819)
                     /locus_tag="PLN77_04985"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRM43666.1"
                     /translation="MRDKMSLQHCLVLTVATGAVWMDLRTRRIANEWIITAWIAGLVT
                     QLIRYGAAGAGIFLFGMLFPILALYILFYFHMLGAGDIKLLSAVGGFLGVPAILKCMI
                     VSFLSGAVLSIGIILVCGNLPQRLTKFFNYFQTYFTKRKYQKKTEPVPYYDGKWGMEC
                     IHFSVPVLMGVLLWIGGFY"
     gene            complement(8147..8629)
                     /gene="ybaK"
                     /locus_tag="PLN77_04990"
     CDS             complement(8147..8629)
                     /gene="ybaK"
                     /locus_tag="PLN77_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRM43667.1"
                     /translation="MAKEKEIKTNAMRILDRQKIKYEVLQYECDEFIDGLHTAEKTGA
                     PVEQSFKTLVAQGKSKQYYVLVLPIAEEVDLKKAAKAVGEKSIEMIHVKDITAVSGYV
                     RGGCSPIGMKKQYPTVIQECAAQFDKVYVSGGRIGTTLCMAPEDLKKVSRAEYADFIQ
                     "
     gene            complement(8629..9501)
                     /locus_tag="PLN77_04995"
     CDS             complement(8629..9501)
                     /locus_tag="PLN77_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HRM43668.1"
                     /translation="MSTTRLFDQDAYLGEFEATVERSVCEGGMYRVLLDQTAFFPEGG
                     GQPADEGTLDGIFVTDVQEIDGEIWHTVEAPLEPGKTVVGKLDFEKRFSNMQNHCGEH
                     IVSGIVHRIYGFNNVGFHMGSDVITVDFDGVLTEEQLYDVEQEANEAVLRNVPVTISY
                     PSKEELETMDYRSKKEIEGQVRIVTIEGCDSCACCGTHVAKTGEIRLIKILSAQKYKG
                     GVRVTMLSGEKAYADYCLKHINTLGIARLLSVKPEEAGDAVVRLKQKNIEMKKEIKQL
                     KKELYALQGAPERK"
     gene            complement(9521..10048)
                     /locus_tag="PLN77_05000"
     CDS             complement(9521..10048)
                     /locus_tag="PLN77_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HRM43669.1"
                     /translation="MKVLIVVDMQNDFIDGSLGTKEAVGIVDRVAEVMDSFDGAVICT
                     RDTHFADYLETSEGRKLPVEHCIEGTKGHALQDTIQKMAEKKQAVVMNKITFGAKNLP
                     EEIEKLAGGVPESIELVGLCTDICVISNAMLLKAFYPEVPISVDASACAGVTPQSHKN
                     ALEAMKMCQIDIVNE"
     gene            complement(10076..10897)
                     /locus_tag="PLN77_05005"
     CDS             complement(10076..10897)
                     /locus_tag="PLN77_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM43670.1"
                     /translation="MNYQVLDVKVKDTEETAKLYTYFLDNSREIAPERVRPVIVICPG
                     GGYEWVSEREAEPIALKFVGAGYHAVVLHYSVAPAAHYPTALRQLAWTVAHLREHAAE
                     YHIDPNKVVVAGFSAGGHLAASYGVFWKKKTFLAEELGVDAEQLRPNGLLLSYPVITS
                     GPKAHRGSFTNLLGDRYGELVDEMSLENQVSLDTPKTFLWHTAPDDCVPVENSILFFT
                     ALHALEIPVEMHIYPVGGHGLGLASEETQSYEYQNAVREECQSWISLALSWMQHL"
BASE COUNT         2480 a         3276 c         2216 g         3176 t
ORIGIN      
        1 cctcctttcc gcaaacccga gtttctgttt accgtccttc tttccggtct cacccggaaa
       61 gaagacacct gctctgctca tatccggctt tcttacgccg cctttttcgc gcatttcgag
      121 cactgcggca gtccatccgt ctgttccagt gctacaagtt ttactttacg tttcagccct
      181 ttgcaggaaa gactgttatg ataccggttt ccgtatttcg tatagtaaac ttccgcaccc
      241 gcagcgccat ttcccacgca ctgttcgcat tccttatatt tttctccatc ctgattccgt
      301 ttccacgcaa gcgcacccgg tgaggaaacc tggatcgaaa gattgatatg actgcacgac
      361 gaatcggtat gatacacact gccgttctca gtcacccaca ccatctgttt tgcttcgccc
      421 tcttcttcat ccgcctcctc ttctgtcttt ccgcagaaca gatgcacttt cgcacacgct
      481 gcaattcccc ggttccgcac cggaacgagg cggtttccca ttttcggcca gtaaatcgct
      541 ttcagttcca gccatccatt tttcacacgg cttcctgcca gtgtcaccat tccattttcg
      601 cggatttctt ttggaatctt tgcttctgca taagcaatgc acgcggccgt ttccggtatt
      661 tcatacgttt cttttcccgt ctcatccacc gcgtaggcac agacggaaag ataccgcgcg
      721 gactcctgca gagataccgt cagcattcca tgcagccggt aaagatccac gatttttaat
      781 aacacaagaa ccgcgatcag aaaaacagga agtaccagtg aagcttccac cgtcaggctt
      841 gccctgttcc attttcgttt tacaaaaggc tgctctcctg atccggattt ataaaaattc
      901 ccggaagagt ggataagttt tttgataagg ttccagaaat aaatgtgccg ttcagaagaa
      961 cacccttttg tatccctgcc ggagtcatcc catttttcag gctgctccct gcatctcata
     1021 tccataattt ctcgtaatct catactctcg tttcccaatt accgccgtaa aaccggcttc
     1081 cagttcatcg acacagcaat ccagtcggaa acccggctgc tgctgttctg tccgcatatt
     1141 gtattccacc agatccatcg ctccaaacgt taccgctttt cccgatttct gcatcaaaag
     1201 cagccgcaga taatcggaat actccatgcc gcccggtgca ttttttctgc tctgatcccc
     1261 tgtttccagc agtttcggaa gctgttccag agaaagctgc cacgattctg ccgttttgaa
     1321 cagtgctact tttccaccat ccagcagttc ccgcagatcc agaatgcttt ctgcaaatgc
     1381 ccagcacacg gcaagtgcca ttttcacaac cggcatcgcc gccggaactc caaacgacgc
     1441 ggcaattacc cccgccattg cttccatctc gccctgcttc tgcgcatctt tcgccagata
     1501 caccagattt gccgcctccc gcagcgccag cagacggttc acggttccct ccagattatc
     1561 ctggtcactg ttttttccgc agagcacata ttccacctgg cactgcagcc cttccttctt
     1621 ctccttgctg gtatagcacg gaaatttcca cagaatatat tcggacgaaa tcatcttatc
     1681 cacaaagctc agttcttttc cacactctgc catgccaaac ccgctctgca gctcccgttt
     1741 tgatgcaaga aacgatacat ccgtttcccc ctgcgacagt gcctgccctt ccgggaggac
     1801 cagtcctaaa attcccattt tcattacttt ttgaatgacc tcaatcggat tcacaaaatc
     1861 ttccggtacc ggctgcttct gatccggttc cgaaggcgtg ccgtttccag attctccttc
     1921 accttctgtt ttttctgttc cctcttctgg ctgtttggat tcttcatact gttttaaaac
     1981 ctcctcttcc tcgtaaccgc tctgtccgtc ctcctgacgt ttcacggttt cctgttctcc
     2041 tcttaatttc tgggaaagct gctgtattcc ataagttcca aggctggctt tcattgcctc
     2101 acagatctga cgggagaaag cacttccctc ctcgtctgtc gcaagacgat agccgccgag
     2161 ataaacttct gtaacctgtc cgccagataa actgcctgat agaattccca tccccgatgc
     2221 agtgggggac agaacttctc ccgcagtttc tgcgactttt ctgctcatgg tttcaggttt
     2281 ccagacaccg ctcttatccc cgccgttata aaaaaacagc tcgtactgtc ccagcagtgt
     2341 tctgtcatac ccggcaaaca gagaatacag tccctgttcc attgcgcagg ccgtcatcac
     2401 ccgtgcatgg gcatttcgca ccgacgcaat tgtcccggaa atcagggaaa gcatcagtat
     2461 cagaacgaga cacaaatatg ctgttatgct tccggctgcc catcttttct gtctttctgt
     2521 ttccctcatg ggatacagct ccttccactt tacttttact ttttcgaact ttagatgccg
     2581 tggatcggat aaaggatata ccgagacaca gatatgcaaa tgacatatag gtccgtaccg
     2641 gcttctaaat ttttctttgg agttccgtat tggacatttt cccgtttata cggtgttgct
     2701 ctggtttgtg accttctcca tcagactgtt gaccagactc acaagctgct ttttaaagat
     2761 tagtacgagt ccgatcagca caacgagaat cagaatcact tccacaacac ccaccgcatc
     2821 ttcctctttc cagaactccg atatcagctt cataattccc acctccctct ctttaccagc
     2881 tgttgtgggg ataaaaacat cccctatgca taaaaggaca aaaatgccgg aaccgtgagt
     2941 atgaccaata caaccgcaag catcatgccc atcggaagaa gaagctttgt cgatgccttt
     3001 tctcctgttg ttttcgcctg ccttcttctc tcctcaaagg cctcctgcga ttcctgtttc
     3061 aataattcca gcagtccttt actgcctttt ttcagattct gcgtcagcag aacagcaagg
     3121 gaacgatacg ctccggttcc catacgcatt cccatgcgct ggtacgcttc ctgctcggaa
     3181 acaccgtttt ccatctcctg gcaggtaaat acaatctctt cataggcaaa acgtttctgc
     3241 ccgccaagcg ccagattttt ccggtaatcc actgcaatcc gctcgagtac tttccgcatc
     3301 ccaagcccgg cacccagaag aagaaccatt ttgctgacaa tttcgggata atcccgcagc
     3361 atctgttctt ctcttttctg ccttgctttt tcttgttccc ttccttttgc cgcccagcag
     3421 actgctcctg ccgccgcggc aagtgccata agttccagac ctgtcagatc cggcactttt
     3481 gaccatgtta cagtttctcc gttcagtgtc tccggcagat aataggctgt ttcttcggga
     3541 tgtgcttcgt tttctttttc cactgctttt ttcagtgccc taagaaccgc atcccgccct
     3601 tttatctccg gtggatagac ctgaaccttc cgtatatatt ctgttgtttc cccctgaagt
     3661 tccagttccg ctttcagcgt aacctccact ccttcttcgg gaatctcgtc tgacagcaaa
     3721 ccccggctgt caatcaacaa cggcctgtct gtttcccagc taagcgccac cgggcttccc
     3781 ggtatttccg taaccagatc catgtttctg tttaccgtat gaaaagacag attctcacct
     3841 aagatgattt catccagcgc ctctatcgcc gcactgaacg cctcttctat ctcttctgcg
     3901 ctgtaagctc ttgcaggtac agacagtgta attctttccg gtttctgatt tccaacagaa
     3961 acctcaagtt tttcctctgt cggttggctg cctaaggcac cccgttccag ttgaagttcc
     4021 gctttttcat ctcctgaaac ttttcgcact ccgatcggaa ggcagagtgc agtgcccgct
     4081 ataaaccctg tcgctgcaag gcagaaaatc cgtctcacca atttgtgatc ttccgccttt
     4141 tttctcatcc ggaaatacca gatgaacagc cccgcccatg cggtcagaca aatccacaac
     4201 atttgattcc tccccttttt tgcggacgaa tcgttatgat tcgcagtctt actttttaaa
     4261 cttcaattgc ttcttaaaac tcaatgtcca gaatcttctt tccaagcacc catgccgcca
     4321 gatacacggc aaggcatccg ctcatgaaga aaaccccgat tacattccca tacatcacac
     4381 ttaagaattc cggaaaggaa agctgtacat atcccagaat tccaaacggc acaacactca
     4441 taatattctg ctcgaaccgc cgggaagaaa cgcccgcacg gatttcccgc atcgtgtcaa
     4501 ttctcgtaca gaaaatatcc cacgtattct gaaagatctc gcagacattt cctcctgtcc
     4561 gcttcgcaat caccagtaca cttgcaaacg tcgcaatatc gtcaatgcgg cttcgcaccg
     4621 caagatcctg aaacagttgt tccaccggca cggaaatcat cattttatga cgcatccggc
     4681 gcagttcctg taccagaatg ttttcttttc ccagcgactg ttccagctgt cctgccgcct
     4741 ccagcactgc attttccatg gaatagccgg aacgcagtgc cgtgtgcatc cacgcaatca
     4801 gatcccggaa gctgtcgtac agctcctctt tccgtctctg aatccggttt ttccgatacg
     4861 tgtgaaaaca tacgattcca accggaacca cagcaatgca tgcccaccac gcccggtaac
     4921 agagcaggtt cacagcggcg gcaatcccaa gactgattcc cgctatttcg agatattccc
     4981 gaccggtaaa ttcccaggtc tgatagtcga tccccttcgt ctccttctcc tctgccatgc
     5041 ccgtctttgc ctggcttccc gcacgtattc tcatcctgtt ttcccctcca gtttttctct
     5101 gtgtaccagt tcgcctgtct tttgcaggcc tctcccatat tctcttcgaa acaaagaaac
     5161 cgttttgatt tcgtcctcct ccatccctgt gatctctgcg atctcctcca cccgacggct
     5221 tccatcctcc ccgcgtccta aatgcacgag aatttcgatt cccgatgcaa tctggcggcg
     5281 gattgcttcc aacgggagct gcactcccat cagcaccatc gtttccagac gggagatcat
     5341 atctttcacg ctgtttgcat gtgccgtgct caaagatccc gcatgcccgg tattgagtgc
     5401 ggataataat tcaaacgttt ccgctccgcg cacttcgccg ataattacgc ggttcggccg
     5461 catacgcaaa gctgcgcgga tcagatcgcg gatcgtaatt tctgttcctg attccagatt
     5521 tgcctctttt gcttccagcc gcaccagatt ctccacaccc tggatctgca gctctgcatt
     5581 gtcttcgatg gttatgatgc gctcgccctt tggaatgtag gcggaaagcg cattcagaaa
     5641 tgtcgttttg ccagatccgg tcgccccgcc gatcatcatg ctgtagcggg tccggacgag
     5701 agcctgcaga aactctgccg cctcttttgt aatactcccg atggagacca gaaattccat
     5761 cgtcaccgcc tgctccggaa agcggcggat ggtcagaatc ggtccgttta atgcgaccgg
     5821 tttcatgaca acgttgacgc ggtctccatt ttccagccgt gcatccacaa tcggattctg
     5881 ttcattcacc acgcggttgc accggcctac aatctgctga atcacatcct ccagcttttc
     5941 cgcggaggta aagcttttgc cgtatctctc gattctgccg ttccgttcca cgaagatatg
     6001 ctgataaccg tttaccataa tttcggtaat tcccgcatct tccagcagct cctgcaggac
     6061 atcgagcctc cgcacactgt agaacagctg tctgcgcagc tccgccttct gtgccaccgg
     6121 aagataccgt gtttcttctt cccctaaaat cagaccgtca atctcttcga gaacctctcc
     6181 gtcatccggt tcggatgcga tatcgatccg ctccatcagt tttctccgca gaatttccgg
     6241 cattgtcatg tttcttctcc tcttctgccg tcccgcagaa tctgacgcac atagtcgccc
     6301 agctcgctcc agacaagctc ctcaaagtag gcttttcccg tccggtttgc cgtgtggaac
     6361 ggaacgtgga ttttccgcat cttgtccagc accgactggc agtcccaccg gcgcagcaaa
     6421 ttttcaaact gctccatttt tgccgcagaa atcacatcat tccttatcgg aacgtaaatt
     6481 tcattgcaaa aatccaggat ctgatacaaa tccgccacgc catccccgag gtcgagaatc
     6541 agcacttcat aactgctgtt cttctcaatc tcctgaaaca gccatttcca ttcttcatat
     6601 tttgcagtct gaatgtccat cggcgatgcg gcgggcggca gataatccag gttctgcatc
     6661 gactggacca ttccgcccag tttgtagatg atgccctgat tttcctgtct cgcataatac
     6721 agaatatcgc tcatccccct gtcgtaactg cattccagca gccgttcaaa tccggaatag
     6781 ctctccatat tgagatacag cactgcccgc tcttttgcca gaatctgccc catcgtcagg
     6841 gcaaacgagg tcttctgcgt ccgtccgacc ggcgaataaa tgccgatgat gcgcatttcc
     6901 tttttcggca gcagtgcagg cgatgtatcg acaccatttt ctacgccgta gcacgccatg
     6961 acctcgcgga tcaccttttc cgacgactgg tatttgtaga ccgccggata ctgatccagc
     7021 accggactgt gtacgccctc agacagaatg ataatctgac ggatcggata ccggcggatc
     7081 tcttctttca tcgctttatc cgaaatcaga agaatctctg tttcgttatt ctttgcgaac
     7141 tccagaaaca tctccgcact ggaaaatgca tgcacttcga acggaatatt tctcttctgg
     7201 ttcagatatt ccataaaatt gcgtgcatat tccatttccg tgtcacagac tgcaaaaaca
     7261 ctctttttct ttgcagtatt caataaaatc ccccaatcca gagcagcact cccatcagca
     7321 cgggaacgga aaaatggatg cactccatgc cccacttccc atcgtaatag ggaaccggct
     7381 ctgttttttt ctggtatttt cttttggtaa aataagtttg gaaataattg aaaaatttgg
     7441 taagacgctg cggtaaattt ccgcagacga gaataattcc gatagataag accgctccag
     7501 acaggaacga cacaatcata catttcagga tcgctggcac accgagaaag cctccaacag
     7561 cggataggag tttgatatct cccgcgccca gcatatgaaa atagaacaga atgtaaagtg
     7621 cgagtatcgg gaaaagcatc ccaaaaagaa aaatacccgc tcccgcggcg ccatagcgga
     7681 tgagctgtgt aaccagcccg gcgatccatg cggtgataat ccattcattt gcaattctgc
     7741 gtgttcttaa atccatccat accgcccctg tcgcaactgt cagtaccaga cagtgctgca
     7801 aagacatttt gtctctcacc tcccgtttcg tttgggtcaa cttgattgtg aggtcattat
     7861 agttgcctct tttcgatttg tccagcggtt ttcggcaatt ttttccggtt tgtgaattgc
     7921 ctccaaaaac gggatgaaaa tttctcacac caaaaagaaa aatcgtgtga aaggttttcc
     7981 gtttttcgcg gattcgtaga atcgtatcgc aatttcaccg ctgctttttg gctattattt
     8041 tatgatcctt ttttgaaaga aattttccag ccgcatcaca gcaaaaagaa acagaccaga
     8101 acttttttct tcgttctggt ctgcttccat acagtttttt ctgtttttac tgaataaaat
     8161 ccgcatactc cgctctgctg acctttttca gatcctccgg cgccatgcac agcgtggttc
     8221 cgattcttcc cccgctcacg taaactttat caaattgtgc cgcacattcc tgaatcacgg
     8281 tcggatactg ttttttcatt ccgatcgggc tgcatccgcc gcggacgtat ccgctgactg
     8341 cagtgatatc tttgacatgg atcatctcga tggatttctc ccccaccgct tttgcggctt
     8401 ttttcaggtc tacttcctct gcaatcggaa gcaccagcac gtaatactgt ttgcttttgc
     8461 cctgcgccac aagcgtttta aacgactgct cgaccggcgc gccggttttc tctgctgtgt
     8521 gaagcccgtc gataaattca tcgcattcat attgaagaac ttcgtatttg attttctggc
     8581 ggtccagaat ccgcattgca tttgttttga tttctttttc ttttgccatt attttctctc
     8641 cggtgctccc tgcagtgcat acagttcttt tttgagctgt ttgatttctt ttttcatctc
     8701 aatatttttc tgtttcagcc gtacgacagc atccccggct tcttccggtt ttacagaaag
     8761 taaacgtgca atgccgaggg tattgatgtg ttttaagcag taatccgcat acgctttttc
     8821 accggaaagc atggtcacgc ggacgccgcc tttgtatttc tgggcactta agattttgat
     8881 cagacggatt tcccctgttt ttgcaacgtg ggttccgcag cacgcacagc tgtcgcagcc
     8941 ttcgatcgtc acaatccgca cctggccctc gatctctttt ttgctccggt aatccatggt
     9001 ttccagctct tcctttgacg gataactgat cgtaaccgga acatttcgaa gcacggcttc
     9061 gtttgcctcc tgctccacat cgtagagctg ctcctcggtc agaacgccgt caaaatcaac
     9121 ggtaatgaca tcacttccca tatggaatcc aacgttgttg aaaccgtaaa tccgatgcac
     9181 gatacctgac acgatatgct ctccgcagtg attctgcata ttggaaaaac gtttttcaaa
     9241 gtcaagtttt ccaactaccg ttttgcccgg ctcgagcggt gcttccacgg tatgccagat
     9301 ttcgccgtcg atttcctgta catccgttac aaaaattcca tccagtgttc cctcatctgc
     9361 cggctggccg ccgccctccg ggaaaaatgc ggtctgatcc agcagcacgc ggtacatgcc
     9421 gccctcgcag acgctccgtt ctacggttgc ctcaaattca cccaggtacg catcctgatc
     9481 aaataatctt gttgtgctca taatttttcc tcttttccgt ctattcgttt acaatgtcaa
     9541 tctggcacat tttcatagct tccaatgcat ttttatgact ctgcggcgtt acgcccgcgc
     9601 acgcggatgc atcgaccgaa atcggcactt ccggataaaa tgctttcagc agcatggcat
     9661 tggaaatcac gcagatgtcg gtgcagagtc ccacgagttc aatggattcc ggcacgccgc
     9721 ctgccagttt ttcgatctcc tccggcagat tttttgcacc gaaggtgatt ttattcatca
     9781 ccacggcctg ttttttctcc gccattttct gaattgtatc ctgcagtgca tgacccttcg
     9841 ttccttcgat acaatgctct accggaagtt ttctgccctc ggacgtttcc agataatccg
     9901 caaaatgggt atctctggtg cagataacag ctccgtcaaa cgaatccatc acctctgcaa
     9961 cgcggtcaac aatcccgacc gcctctttcg tgccgagact tccgtcgata aaatcattct
    10021 gcatatctac tacaattaat actttcatct ttgttctccc ttctgggaca tttttttata
    10081 aatgctgcat ccagctcagt gccagtgaaa tccagctctg gcactcttcg cgtactgcgt
    10141 tctggtattc atagctctgt gtctcctcgc ttgcaaggcc aaggccgtgg ccgccgaccg
    10201 gataaatatg catttctacc ggaatctcca gtgcatgcag tgctgtaaag aacagaatgg
    10261 agttttctac cggcacgcag tcgtccggcg cggtatgcca caggaaggtc ttcggcgtat
    10321 cgaggctgac ctgattctcg agagacatct cgtctaccag ctcaccgtac cggtctccca
    10381 gcagattggt gaaggatccg cggtgcgctt tcggaccgga tgtgataacc gggtaggata
    10441 acagcagacc gttcggacga agctgctctg cgtctacgcc aagttcttct gcaaggaatg
    10501 ttttcttttt ccagaacaca ccgtacgatg ctgccagatg gccgcctgcg gaaaatcccg
    10561 ctactactac tttgttcgga tcaatatgat attctgccgc atgctcccgc agatgtgcaa
    10621 cggtccaggc aagctggcgg agtgccgtcg gatagtgagc tgccggagca acactgtaat
    10681 gcagaacaac agcgtggtaa ccggctccga caaacttcag cgcaatcggc tctgcctcac
    10741 gctcggacac ccattcataa ccgccgcccg ggcagatcac gatgaccgga cgcacgcgtt
    10801 ccggagcaat ctcacgcgag ttgtccagaa aatacgtata taattttgca gtttcttctg
    10861 tatcttttac tttgacatct aatacctgat aattcataag ttcttccttt cccccaattt
    10921 ttccgggtac ggccacagcc gccagttttt actctgttgt cattataccg tatcggcgca
    10981 aaatatcaag cgtatcgcac cagacacttt tttcttttca aatccaattg ctcatagtat
    11041 aataaattta tatttgcatg tttttgagcg gcaaaaataa cacataaagg agtatattgc
    11101 aattatggat tccaatctta ttgtccttac cgatcaggac ggaaattc
//
LOCUS       DAOMUF010000060        10989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1437_length_10989_cov_3.033839, whole genome shotgun sequence.
ACCESSION   DAOMUF010000060 DAOMUF010000000
VERSION     DAOMUF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1437_length_10989_cov_3.033839"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLN77_05010"
     CDS             complement(<1..782)
                     /locus_tag="PLN77_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HRM43671.1"
                     /translation="MKIAVTGKGGVGKTTIAATLARLYAEEGHPVLAADVDPDANLGL
                     ALGFPEEILDSIVPITKMRKLIEERTEASADNRFYKINPRVSDIPDKYSKEYNGVKLL
                     TLGTVETAGSGCVCPEHVMLRRIINSLVLHRGDVVILDMEAGLEHLGRGTTEGMDMFI
                     VVIEPGARSIQTYKNVKRLAEELGVHQVHVVANKVRDEKDEAFIQDRIPPEDLLGMVH
                     YNPEIMEADREGSSPYDYSAAAVEEIRKIKTKIDKSNMPGME"
     gene            complement(845..>2699)
                     /gene="cooS"
                     /locus_tag="PLN77_05015"
     CDS             complement(845..>2699)
                     /gene="cooS"
                     /locus_tag="PLN77_05015"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HRM43672.1"
                     /translation="QATNELLENGAKVGADAWQFRVKNQTPHCKFGEQGTCCRICTMG
                     PCRITPKAPRGICGCDVHGIVGRNFLRFTAGGSATHSDHGREICHTLHEADPNGNYKV
                     KDPEKLIRIAKEWGVETEGKDIYDLAHEMSELALLEYGKPFGTQRFLKRAPQHTQEIW
                     EREEIAPRAIDREVACSLHMTHMGCSSLPEALVRQSLRSGLSDGWGGSMMGTEFSDVL
                     FGTPKPIETEANLGVMKEDEVNIIVHGHDPSLSEMICEYADDPEMIAYAKEMGAKGIN
                     VAGVCCTSNEVAMRRGVPMAGNFLQQENVVLTGACEAIVVDVQCIFPALGPLSKCFHT
                     KFVTTSPIAQMPDAEYIRFNAKTAGENAKAIVKMAIENFKNRKPELVHIPHMKQKATV
                     GYSFESIIKTLDKVTNSQVDQTGTLMPLWQCVASGVIRGAVAMVGCNNPKVRPDTAHI
                     ELMKKLIAHDVIIVASGCSAQAAARAGLMDKRAKDLCGAGLKRVCELADIPPVLHMGS
                     CVDISRMMVLVAELAKIGNVNISQLPVVGCAPEWMSEKAVSIGNYVVATGIDTYLGVE
                     PYVSGSTEVTGLLTNGVRDWVEAAYTVEKDIDKLGDAMLARIEEKRAALGV"
     assembly_gap    2700..2709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2862..3632)
                     /locus_tag="PLN77_05020"
     CDS             complement(2862..3632)
                     /locus_tag="PLN77_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM43673.1"
                     /translation="MAHVIAVAGKGGVGKTTLCGLLIQYLCESGKGPVLAVDADANSN
                     LNEVLGVEVEATLGDIREEIARAEMASKNPIPAGMSKADYAEFKFAGALTEEDDFDLL
                     VMGRTQGKGCYCFVNGLLQNQLAKLYPNYPFMVVDNEAGMEHISRGILPGMDTAILVS
                     DCSRRGVQAAARIAELIKECDMKPKQVGLIVNRAPNGVLNEGTREEIEKQGLTLLGVV
                     PHDDQVYEYDCDGKPTVRLPEDSPVRAALKKIVENLDL"
     gene            complement(3671..4603)
                     /locus_tag="PLN77_05025"
     CDS             complement(3671..4603)
                     /locus_tag="PLN77_05025"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRM43674.1"
                     /translation="MKLSEEMKNKTLLSFELFPPKTEKGLQNLPGTIDHLMKYNPSYI
                     SCTYGAGGGNVGANREVCQMIKNAGTTPVTHFTVIKNTKEGIKEQLDQYLADGVDHML
                     ALRGDIPLGETTTCGDFDYATDLVKFVRDTYGDKFEIAVAGSPEGHISCRSLESDIAV
                     LKQKQDNGADYIMTQLCWDMEQFKYWLDAIRKAGITMPVDVGVMPILDQAATINMALS
                     RNGCVMDRELSRMISRHWLFPNPFAAKDAEGKPFDVFYDKKVAEFKEEGIEYTVKQID
                     AYRALGVNGIHLYALNKWKDVSEIIDRSGLCTLV"
     gene            complement(4739..4921)
                     /locus_tag="PLN77_05030"
     CDS             complement(4739..4921)
                     /locus_tag="PLN77_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HRM43675.1"
                     /translation="MCLATVYKNPENTVVCKNVSRIDVDGEYVLIRDILGEEIRLQGK
                     IKMVDLANSIVELDCE"
     gene            complement(4936..>5141)
                     /locus_tag="PLN77_05035"
     CDS             complement(4936..>5141)
                     /locus_tag="PLN77_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HRM43676.1"
                     /translation="NLALLTYMLQHNEHHAAELDEMADKLEKAGMADAAKQIREGVSD
                     FQKGNMRLSLALTLVKEHLKEVK"
     assembly_gap    5142..5151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5152..5293)
                     /locus_tag="PLN77_05040"
     CDS             complement(<5152..5293)
                     /locus_tag="PLN77_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HRM43677.1"
                     /translation="MHLLYDENGNPIPHGAHDEHHHHHDHHHEGEDCGHACNACGSEE
                     KCK"
     gene            5657..6478
                     /locus_tag="PLN77_05045"
     CDS             5657..6478
                     /locus_tag="PLN77_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM43678.1"
                     /translation="MALSIPTITLNDGRAMPLLGLGTYKSTEKEAEKAIVTAAKYGYR
                     LIDTASSYKNEDSIGRGIRASGIPRENFFITTKVWNTAQRVGDIEGAFNRSLDRLRLD
                     YVDLYLIHWPVPGCYPETWRALEKIRESGRAKSIGVSNFEEPHLTALFEFSGIIPAVN
                     QIECHPLWNRKPLIAFCRSHGIAVQAYAPLARGLYLNREIMQQLAEKYVRTEAQIGLR
                     YLVQQGISVIPKSTQPDRIFANADVFDFELSEADMALIDTMDEQLRSASIPDDLL"
     gene            complement(6527..7183)
                     /locus_tag="PLN77_05050"
     CDS             complement(6527..7183)
                     /locus_tag="PLN77_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM43679.1"
                     /translation="MREDTIEICHLKKAYGEHVIFSNLTMKLKKGAVTCLMAPSGAGK
                     TTLLRILAGLEQADGGKIGGLEALRKSMVFQEPRLAEELTAAANIQLAVRRRMFGKEK
                     RSSRYLVEEMEELGLSGCENQAVSELSGGMRQRVAVLRAMRMDADFLLLDEPFRGLDA
                     GTKKKTMNYIRRKGKEKTMLLVTHDLEEAEAMGDWICTCSRLDGILEIKRNRKSSTEI
                     "
     gene            complement(7180..7962)
                     /locus_tag="PLN77_05055"
     CDS             complement(7180..7962)
                     /locus_tag="PLN77_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM43680.1"
                     /translation="MTSIIPNKNKKEKQRKTKLWPIVFWLMIWQAASVFVGQEILLAS
                     PAAVLKRLFTLVTEPVFWQSLLFSGARIGAGFLLGAAAGIAGAALAGRFSIAEELLTP
                     LVAAVKAVPVASFVILILIWVSSKNLSVVISFLMVFPILYTNTRNGLRELDTALLEMT
                     DVFQVPRPVRLRWVILPQLYPYIRTGCSLSLGLCWKAGTAAEVIGIPNRSIGEHLYQA
                     KIYLDTPGLFAWTAAIVYVSFCLEKAVLKGMDLAGKRMLVMK"
     gene            complement(7940..9022)
                     /locus_tag="PLN77_05060"
     CDS             complement(7940..9022)
                     /locus_tag="PLN77_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43681.1"
                     /translation="MKKKRKMGFAAAILAMVLLFAGCGQTETKTESSGTKSTGSESTV
                     SESTQTESSTAASETEDETPVHVLALKGPTSMGMVKMMSDNDSKESPADTFELAAAPD
                     EVSAKLVQGEVDIAAVPANLASVLYNKTNGGVQVLAVNTLGVLYIVEDGDTVHSIADL
                     KGRTIYAGGKGATPEYALNYILEKNGLVPGEDVTIEWKSEHAECVAALAEDADGIAML
                     PQPFVTTAQTKKETLRTALDLTEEWNKIQESEGTASTLVTGVTVVRTAFAKEHPEAVA
                     DFMEAYQASVSYVTENTDEAAKLIGDYDIVPEAVAKKALPACNIVCITGDEMKEKLSG
                     YLGVLKDQNPEAVGGELPGDDFYYSE"
     gene            complement(9048..10571)
                     /locus_tag="PLN77_05065"
     CDS             complement(9048..10571)
                     /locus_tag="PLN77_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRM43682.1"
                     /translation="MRGVETRIQEIRHAIFTEVAKMAYDVDSPVEKTIEEIPYRVIPG
                     EVGNFRNDVFLERAIVGERLRLAMGLPCRSAAEHAPISDNIKLADQAETYYTPPLINV
                     IKFACNACHEKRVLITEGCQGCLAHPCVEVCPKKAITLDRTNGRSYIDQDKCVKCGQC
                     AKVCGYQAIIIQERPCARACGMDAIGSDENGKADIDYEKCVSCGQCLVNCPFGAISDK
                     SQIFQVIRAIQSGERVYAALAPAFVGQFGPKVTPGKLRAAMKKLGFADVFEVAIGADL
                     CAAQEAEDFVNEVPEKLPFMATSCCPAWSVMAKKLFPDYASCISMALTPMTLTARLIK
                     KHNPNAKVVFVGPCAAKKLEAMRRSVRSEVDFVLTFEEMSGIFDARQVDVSALEEDPD
                     GVSDASVDGRNFAVSGGVAKSVENVIREKYPDREIKMANAEGLKECRKLLTMAKAGKY
                     NGYLLEGMACPGGCVAGAGTMQSIKKSQAAVNKYAAQAKHKISSQTEYVKELDKLVD"
     gene            complement(10759..>10989)
                     /locus_tag="PLN77_05070"
     CDS             complement(10759..>10989)
                     /locus_tag="PLN77_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRM43683.1"
                     /translation="GQGRTMQQAMDEVQMIVEGVYSAKAGLTLSKKFGIETPIIQAVN
                     QVLFENKNPKQAVDELMQRMKKDELAPDLWER"
BASE COUNT         2374 a         3026 c         2436 g         3133 t
ORIGIN      
        1 ttttccatgc caggcatgtt tgacttatcg atttttgttt ttattttccg tatctcttct
       61 actgcagccg cgctgtaatc atatggagac gaaccttccc tgtccgcttc cataatttcc
      121 gggttgtagt ggaccatgcc cagaagatcc tccgggggga tacggtcctg tatgaatgcc
      181 tcatcttttt cgtcgcgtac tttgtttgcc acgacatgaa cctgatgaac gccaagctct
      241 tcggccagac gtttgacatt cttataggtc tgaatacttc ttgcacccgg ctcgatgact
      301 acgatgaaca tatccatgcc ctccgtcgtt cctcttccca gatgctcaag gcctgcttcc
      361 atgtcgagga tgacaacatc ccctctgtga aggaccaggc tgttgatgat tctccgcagc
      421 atcacatgct ccgggcagac acagccgctt ccggccgtct ccacggtgcc aagcgtcagg
      481 agtttgactc cattgtactc tttgctgtat ttatcgggga tatcacttac tctcggattt
      541 atcttgtaaa accggttgtc tgcacttgcc tcggtgcgct cttcgatcag ttttctcatt
      601 ttggtaatcg gaacgatcga atcgaggatt tcttccggaa atccaagcgc aaggcccaga
      661 ttggcatccg gatcgacatc tgcagccaga accggatggc cttcttctgc ataaagtctt
      721 gcaagggtcg ctgcgatggt tgtttttcct acgccaccct ttccagttac tgctattttc
      781 attttacacc ttttcacttc aaaaagggcg gtctcttttt acgtcaggga ccgccctgtc
      841 ctttttatac gccgagtgcg gcacgtttct cttcgattcg tgcgagcatt gcatcgccca
      901 gcttgtcgat gtctttttct actgtgtaag ctgcctcaac ccagtcgcgg acaccgttgg
      961 tcagaagtcc tgttacctca gtggatccgg atacgtacgg ctctacgcca aggtaagtat
     1021 cgataccggt tgctacaaca tagtttccga tggaaactgc tttctcagac atccactccg
     1081 gagcacatcc gacaaccgga agctgggaaa tattgacatt accgatttta gcaagttctg
     1141 ctacaagaac catcatacgg gagatatcaa cgcaggatcc catgtggagt accggcggga
     1201 tatcagccag ctcgcagaca cgtttcagac ctgcaccgca gagatcttta gcacgtttgt
     1261 ccatcagacc tgctcttgct gctgcctgtg cagagcatcc ggaagcaacg atgataacgt
     1321 cgtgcgcgat cagcttcttc atcagctcga tgtgagcggt gtccggacgg actttcgggt
     1381 tgttgcatcc taccatggca actgcaccgc ggataacgcc ggatgccaca cactgccaca
     1441 gcggcatcag agttccggtc tggtcaacct gggaattggt tactttatcc agagtcttaa
     1501 tgatggactc aaaagagtat cccacggttg ctttctgctt catatgcggg atatgtacca
     1561 gctccggttt ccggttttta aagttttcaa ttgccatttt tacgatggct tttgcattct
     1621 cacctgctgt ttttgcgttg aaacgaatgt attctgcatc cggcatctgc gcaatcgggc
     1681 tggtggtaac gaatttagta tggaaacatt tgcttaaagg tccaagggcc gggaaaatac
     1741 actgtacgtc aacaacgatg gcctcgcagg caccggtcag taccacgttt tcctgctgca
     1801 ggaagttacc tgccatcggc acgccgcggc gcattgctac ttcattggaa gtacagcaga
     1861 cacctgctac gttgattccc tttgctccca tttcttttgc atatgcgatc atctccggat
     1921 cgtctgcgta ttcgcagatc atctcggaaa gtgacggatc atggccatga acgatgatat
     1981 ttacttcatc ttctttcata acaccaaggt ttgcctcggt ttcgatcggc tttggagttc
     2041 cgaacagtac gtcagagaac tcggtaccca tcatggagcc gccccatcca tcggaaagtc
     2101 cggaacgaag ggactggcga acgagtgctt ccggaaggga agaacatccc atatgggtca
     2161 tatgaagaga acatgcaacc tcacggtcaa tcgcacgcgg tgcgatttct tctctctccc
     2221 agatttcctg tgtatgctgc ggagcacgtt tcaggaatct ctgtgttccg aacggtttac
     2281 catactctaa aagagccagc tcggacatct catgtgccag atcatagata tcttttcctt
     2341 ctgtctctac gccccactct tttgcaatac ggatcagttt ctccggatct tttactttgt
     2401 agtttccgtt tggatcagcc tcatgcagtg tgtggcagat ctcacggcca tgatcggaat
     2461 gtgttgcaga accgcctgct gtaaagcgca ggaagttacg tcctacaata ccgtgtacat
     2521 cgcatccgca gattcctctc ggtgcctttg gtgtaatacg gcaaggtccc atcgtacaga
     2581 tacggcagca ggttccctgc tcaccgaatt tacagtgcgg ggtctggttt tttacacgaa
     2641 actgccatgc gtcagcgcca acctttgcac cattttccag gagctcattc gtggcctgtn
     2701 nnnnnnnnna cgcttgttaa tttgaattcg ctcatcttgt cctcctcaag attgtatttt
     2761 aatattttta tggtaaaaac atcctcccat gggggatatt ttacttttga aatatccctc
     2821 cgtctgcagc gtgtacaaag cggagggcag attctctgtt tttataaatc aaggttttct
     2881 acgattttct ttaatgccgc gcgtaccgga gagtcttccg gcagacgtac ggttggtttt
     2941 ccgtcacagt catattcata aacctgatca tcgtgcggaa caacgccaag gagagttaat
     3001 ccctgttttt caatttcctc acgggttccc tcatttaaca cgccattcgg cgcgcgattt
     3061 acgatcagtc ctacctgctt cggcttcata tcgcattctt tgatcagctc cgcgattctc
     3121 gcagcggcct gtactcctct tcttgagcag tcactgacca gaatagctgt atccatgccc
     3181 ggaagtatac cacggctgat atgctccatg cccgcctcgt tatccacaac catgaagggg
     3241 taattcggat acagctttgc aagctgattc tgcagaagtc cattgacgaa gcagtagcag
     3301 ccttttccct gtgttcttcc catcacaagc aggtcaaaat catcttcctc agtcagagcg
     3361 cctgcaaatt taaattccgc ataatctgcc ttgctcatgc ctgccggaat cgggtttttg
     3421 gatgccatct ctgcacgggc aatctcctcc cggatatctc caagggtagc ttctacttct
     3481 acaccgagta cctcattcag gtttgagttt gcgtccgcat caactgcaag aacaggacct
     3541 tttcctgact cgcagagata ctggatcagc aagccgcata atgttgtttt tccaacgccg
     3601 ccctttccgg cgacagctat tacgtgtgcc ataaaaatcg gatgcctccc aaattcaagt
     3661 aattaattca ttaaaccagt gtgcaaaggc cggatctgtc gatgatttca gatacgtctt
     3721 tccatttatt cagtgcatac agatgaattc cattgacacc gagtgcacgg tatgcatcaa
     3781 tctgttttac ggtatattcg ataccctctt ctttgaactc tgcaactttc ttgtcgtaga
     3841 atacgtcgaa cggtttgccc tctgcatctt ttgctgcaaa tgggttcggg aacagccagt
     3901 gtctggagat cattctggaa agctctctgt ccataacaca gccgtttctg gaaagtgcca
     3961 tgttgatggt agctgcctga tccaggatcg gcataactcc aacgtctacc ggcatggtga
     4021 ttcctgcttt acggattgca tcgagccagt atttgaactg ctccatatcc cagcagagct
     4081 gtgtcatgat gtagtcagct ccgttgtcct gtttctgttt cagaactgcg atatcagact
     4141 ccagacttct gcaggagatg tggccttccg gtgaacctgc aacagcgatt tcaaatttat
     4201 cgccgtaggt atcacgtacg aattttacaa gatccgtagc gtagtcaaag tcaccgcagg
     4261 ttgtggtctc gccaagcgga atatcaccgc gcagtgccag catatggtct acgccgtcag
     4321 ccagatactg atccagctgc tctttgattc cttcttttgt gtttttgata acggtgaaat
     4381 gagtaaccgg tgtggttccg gcatttttga tcatctgaca tacttcacgg tttgctccga
     4441 cgtttccgcc gcctgctccg tatgtacagg aaatgtaaga cgggttatat ttcatcaggt
     4501 ggtcgatggt acccggcaga ttctgcagtc ccttctctgt ctttggcggg aacagctcaa
     4561 aagacagaag agtcttgttt ttcatctctt ctgaaagttt cataattcaa tttacctccg
     4621 ctttaatata atatattcct attctaagtc ccggttcgcc tgttctgttt gttccgattc
     4681 gcagaccccg gggtcttaaa accgctttta tagaataact ggttccgtca accaattatc
     4741 attcacagtc cagctctaca atgctgtttg caagatctac cattttgatc ttaccctgca
     4801 gtctgatctc ttcgcccagg atgtcgcgga tgagaacgta ttctccatcg acatcgatgc
     4861 ggcttacatt tttacagaca actgtgttct ccggattttt gtataccgtt gcaaggcaca
     4921 tagcttacgc ctcctctact taacttcttt caaatgttct tttacaagcg tcagtgcaag
     4981 ggacaggcgc atattgcctt tctggaaatc ggatactccc tccctgatct gttttgcggc
     5041 gtccgccatt ccggcctttt ccagtttgtc ggccatctcg tcgagttctg ctgcgtgatg
     5101 ttcattgtgc tgcagcatat aggttaaaag ggcaagattc tnnnnnnnnn nccttgcatt
     5161 tttcttcgga accgcatgca ttgcaggcat gtccgcagtc ttctccctcg tgatgatggt
     5221 catggtgatg atggtgctcg tcgtgagctc cgtgtggaat gggatttcca ttctcgtcat
     5281 ataataaatg cataatatgg cctcctctgt ccccgttttt ctgctctgcc gtccgtttcg
     5341 tccgcttttg ttttcctgaa acatcctgca gattgtacag ggaatgataa ataaatatca
     5401 attcaagttt attatactct atttcatgca caaaatcaat acagcctcca caaaatttct
     5461 gtacaattcg ccgtacaggc tgtcctggca tccttctttt ttcttgcgtg gcttgttatt
     5521 ttttttcatt tcccgattgt gttaaacttt tttaatatat ttggtctgat ttctttaaat
     5581 tttcatgtta tactaaacat aattatgaga gaaatacggc agaatctttt tttgattcaa
     5641 gatacggagg gtttttatgg cactttctat tccaaccatc acattgaacg acggcagagc
     5701 gatgccgctt ctggggcttg gcacttacaa atcgacagaa aaagaggcag aaaaagcaat
     5761 tgtcaccgcg gcaaaatacg gctaccgtct gatcgatacc gcctcctcct ataaaaatga
     5821 ggacagcatc ggccgcggga tccgcgcaag cgggattccg cgggaaaact tttttattac
     5881 cacaaaggta tggaataccg cgcagcgggt cggtgatatc gaaggtgcct tcaaccgcag
     5941 cctcgaccgt ctccgtctcg actacgttga tctttacctg attcactggc cggtgccggg
     6001 ctgctacccg gagacgtggc gcgcactcga aaaaatccgc gaatccgggc gtgcaaaatc
     6061 gatcggcgtc agcaacttcg aggagccgca ccttaccgcc ctctttgaat tttccggcat
     6121 catcccggcc gtcaaccaga tcgaatgcca tccgctctgg aaccggaaac cgctcatcgc
     6181 tttctgccgt tcacatggaa tcgccgttca agcgtatgcg ccgctcgcaa gaggtcttta
     6241 tctgaaccgt gagattatgc agcagctggc agaaaagtat gtacgcactg aagcgcagat
     6301 cggcctccgc tacctcgtcc agcagggcat cagtgtcatc ccgaaatcga cacagccgga
     6361 ccgcattttt gccaacgcgg atgtcttcga cttcgaactt tctgaagccg acatggcgct
     6421 catcgacacg atggacgaac agctccgctc tgcttccata ccggatgacc tgctgtaact
     6481 gcggattcac agcaaaagca gcacagaaaa tctgtttctt tccgtttcag atttctgtgc
     6541 tgctttttct gtttctcttt atttcgagga taccatccag tctgctgcag gtgcagatcc
     6601 agtcccccat tgcttcggct tcttctaaat catgcgtgac cagaagcatc gttttttctt
     6661 ttccttttct gcggatatag ttcatcgttt tctttttcgt ccccgcatcc agtccccgga
     6721 acggctcatc caaaaggagg aaatctgcgt ccatgcgcat ggcccgcaga accgccaccc
     6781 gctggcgcat gccgcccgac agttccgata cagcctggtt ttcacagccc gaaaggccga
     6841 gttcctccat ctcctccaca agataacggg agcttctttt ttcttttcca aacatgcggc
     6901 gccttaccgc gagctggata ttggctgcag ctgtcagttc ttctgccagc cgcggttcct
     6961 gaaaaaccat actttttctt aaggcttcca gtcccccgat ttttccgccg tccgcctgct
     7021 ccaggcctgc cagaatccgc agcagggttg tctttcccgc cccggaagga gccatcagac
     7081 aggtcactgc tccttttttc agtttcatcg taagattcga aaaaataacg tgttctccat
     7141 acgctttttt cagatggcag atttcaatcg tatcttctct catttcatca ccagcatcct
     7201 ttttcctgca agatccattc cttttaaaac agctttttcg agacagaagc tgacgtacac
     7261 gatcgctgcc gtccaggcaa agagtcccgg tgtgtcgaga tatatttttg cctgatacag
     7321 atgctctccg atggatcggt tcggaatgcc gattacttct gctgccgttc cggccttcca
     7381 gcaaaggccg agggaaagtg aacatcccgt ccggatatat gggtacagct gcggcaggat
     7441 cacccagcgc agccgcaccg gccgcggaac ctgaaaaacg tctgtcatct ccaaaagcgc
     7501 tgtgtccagt tcccgcaggc cgttccgcgt gtttgtatac aggatcggaa ataccatcag
     7561 aaacgaaatc acaaccgaaa gattcttcga cgaaacccat atcaggatca gaatcacaaa
     7621 agaagcgacc ggcactgctt tcactgctgc aaccagcggc gtcaggagtt cttctgcaat
     7681 ggaaaaccga ccggcaagtg cagctcctgc aattcccgcc gccgcaccaa gaaggaatcc
     7741 ggctccgatg cgtgctccgg aaaacagcag ggactgccag aagaccggtt ctgttacaag
     7801 cgtaaaaagg cgtttcaaaa cagcggcagg agatgccaga aggatctcct gcccgacaaa
     7861 aacgcttgcc gcctgccaga tcataagcca gaacacgatc ggccagagct tcgtttttct
     7921 ctgtttctct tttttgtttt tattcggaat aatagaagtc atctcctgga agttctcctc
     7981 cgactgcctc cgggttctgg tctttgagta caccgagata accggaaagc ttctctttca
     8041 tctcatcacc ggtgatgcag acaatgttgc atgccggaag cgctttcttt gcgactgcct
     8101 ccggaacaat gtcataatcc ccgatcagtt ttgccgcctc atcagtgttt tcggttacgt
     8161 aggatacgga tgcctggtaa gcttccatga agtctgcaac tgcttccgga tgctccttgg
     8221 caaaggcagt gcgtacaacg gtaacacctg tcaccagtgt gctcgccgtt ccttcggact
     8281 cctggatttt gttccattct tcggtcaggt caagtgcggt gcgcagcgtt tcttttttcg
     8341 tctgtgccgt cgttacaaac ggctgcggca gcatggcgat tccatcggca tcttctgcaa
     8401 gtgctgccac gcactcggcg tgttcgctct tccactcgat cgttacgtcc tcacccggta
     8461 cgaggccgtt cttttcgagg atataattca aagcatactc cggagttgca cctttgccgc
     8521 ccgcataaat ggttctgcct ttcagatccg caatggaatg tacggtatcg ccgtcctcga
     8581 cgatataaag gacaccgagc gtattgactg cgagcacctg cacgccgccg tttgtcttgt
     8641 tgtagagtac gcttgcaagg ttcgcaggaa ccgcggcgat atccacctct ccctgtacca
     8701 gttttgcgct gacctcatcc ggggcagcgg caagttcaaa cgtgtccgca ggtgactctt
     8761 tgctgtcgtt atcgctcatc atttttacca ttcccatgct ggtcggtcct tttaatgcaa
     8821 gcacatggac cggtgtctca tcttctgttt ccgatgccgc tgtgctgctc tctgtctgcg
     8881 tactttctga tactgtactt tctgatcctg tgctttttgt cccgctgctc tccgtctttg
     8941 tctctgtctg gccgcatcct gcaaacagaa gtaccattgc aagaatcgct gccgcaaagc
     9001 ccatctttct tttctttttc acttttgtct ccaccttgct gttcttttta gtctaccagc
     9061 ttgtccagtt cttttacata ttctgtctgg ctggaaatct tgtgttttgc ctgcgccgca
     9121 tatttgttga ctgccgcctg cgactttttg attgactgca tcgtgccggc tcctgccacg
     9181 catccgcccg gacaggccat gccttcgaga agataaccgt tgtactttcc ggcctttgcc
     9241 attgtcagca gtttccggca ttccttcaga ccttctgcat ttgccatctt gatttcgcgg
     9301 tccgggtatt tttcacggat tacattttcc acagattttg caacgccgcc ggagacagca
     9361 aagtttcttc cgtccaccga tgcatcgctg acgccgtccg gatcttcctc aagtgcactc
     9421 acatctacct ggcgcgcatc gaaaatgccc gacatctctt caaacgtcag aacgaagtcc
     9481 acctcactgc ggacagaccg gcgcatggcc tccagttttt tcgccgcaca cggtccgaca
     9541 aagacaacct tcgcattcgg attgtgtttc ttgatcagac gcgccgtcag cgtcatcggg
     9601 gtcagtgcca tcgagataca gcttgcgtaa tccgggaaca gtttcttcgc cattacagac
     9661 caggccggac agcaggatgt cgccataaac ggcagtttct ccggaacttc gttcacaaaa
     9721 tcctctgcct cctgtgccgc acagagatcc gctccgatcg caacttcaaa gacatccgca
     9781 aaaccgagct ttttcatcgc cgcacgcagt tttccaggcg ttactttcgg tccgaactgg
     9841 ccgacaaacg ccggtgccag cgctgcatag acgcgctctc ccgactggat cgcacggatt
     9901 acctggaaga tctgggattt gtccgaaata gcgccgaacg ggcagttgac gagacactgg
     9961 ccgcaggata cacatttctc gtaatcgata tccgcttttc cgttctcatc tgaaccgatg
    10021 gcatccattc cgcaggctct cgcacacgga cgctcctgaa tgataatagc ctggtatccg
    10081 cataccttgg cacactgacc acatttgacg catttatcct gatcaatata ggatcgtcca
    10141 ttggtgcggt ccagcgtaat ggcctttttc ggacagactt ccacgcacgg atgtgcaaga
    10201 catccctgac atccctccgt gatcagaacc cgtttttcat gacaggcgtt gcatgcaaat
    10261 ttgatgacat taatgagcgg cggtgtataa tacgtctccg cctggtccgc cagtttgata
    10321 ttgtccgaga tcggggcgtg ctctgccgcg cttctgcacg gaagtcccat tgcaaggcga
    10381 agccgctctc ctacaatcgc ccgctccaga aacacatcat ttctaaaatt tccgacctca
    10441 ccgggaatta cacggtaagg aatttcctcg atcgtctttt ccaccgggga gtccacgtca
    10501 tacgccattt ttgcaacttc cgtaaaaatg gcatggcgga tttcctgaat tcttgtttct
    10561 actcctctca ttctttcttc ctcctgctgt attttcctcc gcatcttttc agttaaaatt
    10621 attccgcttt ctattactat acttgatttc gaaagtattg tcatcttttt cccgtatttt
    10681 tgctgaaaaa tttcggtctg cacaaaaaag aacctgccgt ctctgtatca tttcacagaa
    10741 gtgacaggtt tcttattttt accgctccca gagatccggg gcgagttcgt cttttttcat
    10801 acgctgcatc agttcatcca cagcctgttt cgggtttttg ttttcaaaca gaacctgatt
    10861 gactgcctga atgatcggcg tttcgattcc gaattttttc gacagggtaa gacccgcttt
    10921 tgcggaatag acgccctcga cgatcatctg aacctcatcc atcgcctgct gcatcgttct
    10981 gccctggcc
//
LOCUS       DAOMUF010000061        10881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1456_length_10881_cov_3.094495, whole genome shotgun sequence.
ACCESSION   DAOMUF010000061 DAOMUF010000000
VERSION     DAOMUF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1456_length_10881_cov_3.094495"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1100
                     /gene="queA"
                     /locus_tag="PLN77_05075"
     CDS             75..1100
                     /gene="queA"
                     /locus_tag="PLN77_05075"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRM43684.1"
                     /translation="MKTSDFYYDLPKELIAQDPLEDRSASRLMHLDKTTGEYKHGHFR
                     DILQYLKPGDCLVINDTKVIPARLYGSKVGTDAAIEILLLKRKENDIWETLVKPGKKC
                     KVGTVISFGDGILTGEVVDIVDEGNRLIQFHYEGIFEEILDQLGEMPLPPYITHKLQD
                     KNRYQTVYAKHEGSAAAPTAGLHFTKELLQQVQDAGVKIAHVTLHVGLGTFRPVKVED
                     VTQHHMHSEFYVVEEDQAKLINDTKAAGGRVIAVGTTSCRTLESATGEDGILKAGSGW
                     TEIFIYPGYRFKMIDGLITNFHLPESTLVMLVSALAGKEHIMAAYEEAVREKYRFFSF
                     GDAMMIE"
     gene            1329..2003
                     /locus_tag="PLN77_05080"
     CDS             1329..2003
                     /locus_tag="PLN77_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004065860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRM43685.1"
                     /translation="MKRDERRKFPYYGILPLILTLTANFIAYFGTRPFTSSWKHYNIE
                     TVLDQQIPVIPWTIVIYFGCYLVWIVNYLIAASREKEFVWRFFAADVLARLVCMAFYL
                     LLPTTNVRPSIPEQGFWNHMLALLYQMDAADNLFPSIHCLNSWFCYIAVRGRREIPRW
                     YQRFSFWAALAVFVSTLTTKQHVIADVIGGALLAEVTWQIAGRTHLGAWYGMILERRR
                     WKRRAE"
     gene            1993..3054
                     /locus_tag="PLN77_05085"
     CDS             1993..3054
                     /locus_tag="PLN77_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRM43686.1"
                     /translation="MQSKKKMIVNTVFLVAIFALTIYGVFHGEDLGAMMEAMRRADVR
                     WLLPGLLCVVFFIWGESIIIWYMMHSFQIPVKRRTCFLFSSVGFFFSCITPSASGGQP
                     MQLYYMKKEKISLPVSTVILMIVTITYKAVLVVVGLGLVLFGQGFLHRYLTEILPVFY
                     LGIGLNVFCVTAMLILVFHPALARTILVFGLKIVEKLHLMKRKESRLKKLEASMEVYQ
                     NTAVYLGTHKMVLVNVLAITFAQRFALFAATWFVYRAFHLSGISFITIVLLQAVISVS
                     VDMLPLPGGMGISETLFLKIFPPVFGSTLLLPAMVLSRGLGYYGELLVSAVFTVVAQL
                     TIGNTKREKRGGTTYDRVL"
     gene            3038..3673
                     /locus_tag="PLN77_05090"
     CDS             3038..3673
                     /locus_tag="PLN77_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRM43687.1"
                     /translation="MIGFYDYTVILTYISFASSISGIFLATRGHFNWAIFCLAFSGLC
                     DMFDGKIARTKKNRTEDEKRFGIQIDSLCDVVCFGVFPIVLCYELGMTHFYSIPILIF
                     YGLAGVIRLGYFNVMEEKRQNETSENRKYYQGLPITSMSVALPLLFVFAPLCRGYFLI
                     WLHLLVLAVGVLFITDFRFRKPTNKELAVLVAVVGVAVLRGLLIWKGGRTI"
     gene            3670..4581
                     /locus_tag="PLN77_05095"
     CDS             3670..4581
                     /locus_tag="PLN77_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRM43688.1"
                     /translation="MRLCAYPYRKQGKNHPKLDLIREENDLQDRFLHMMYSHAAGRAA
                     MQPLVQSFVSRAAGCFLESRLSVPLVAPFVKKNHISLKECTAKQFISFNDFFVRKLKM
                     DARPFSNAPQDFISPCDARLTVYPIHENGKFEIKNTEYTLEQLLRDRKLAKRYEGGTL
                     FLFRLSVDDYHRYLFVDDGVCSTERRIEGVLHTVNPAANDFCPIYKENTRTYTLLQSA
                     NFGTLLQMEVGAMMVGKIVNENAGAARRVQRGEEKGYFAFGGSTVIVLAQKGSAVPEK
                     RIWNYSRLGIETRVRQGETVGTAGRTG"
     gene            complement(4643..5926)
                     /gene="aroA"
                     /locus_tag="PLN77_05100"
     CDS             complement(4643..5926)
                     /gene="aroA"
                     /locus_tag="PLN77_05100"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRM43689.1"
                     /translation="MQFQKAVRPLRGEISVPGDKSISHRAVMFGAISEGTTEVTNFLQ
                     GADCLSTISCFRSMGIEIENTPERILVHGKGLHGLSAPSAVLDCGNSGTTTRLISGIL
                     AGQNFSSTLTGDASIQKRPMGRIINPLTQMGASIRSIPENGCAPLAINGHPLHAISYL
                     SPVASAQVKSCVLLAGLYADGKTSVTEPALSRNHTELMLKFFGADVASEGLTASVLPD
                     PDLHGNLVRVPGDISSAAYFIAAALIVPGSEVLLKNVGINPTRDGMLRVCRAMGADIT
                     LLNEQNSGGEPVADLLVRYTPSLSGTVIEGELIPTLIDEIPIIAVLAATANGTTVIRN
                     AEELKVKESNRLDIMVNELTAMGVSLEGTDDGMIIEGGKPFHGAQIDSRLDHRIAMSF
                     AIASLAAETPTEILRAGCVDISYPTFYQDLARLQA"
     gene            complement(5961..7073)
                     /locus_tag="PLN77_05105"
     CDS             complement(5961..7073)
                     /locus_tag="PLN77_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRM43690.1"
                     /translation="MCDTSEKICIGFIGLGLIGGSIAKTLRKVHPEYKLMAYCRTKAT
                     IEEAIQEGVIDIALEERDDRFADCDYIFLCAPVSTNISYLPFVKGIAKPGCIITDVGS
                     VKGEIHQAVETLGMTHQFIGGHPMAGSEKTGYSSATDYLLENAYYMITPGPDVEIATV
                     SAFVDLVQSLKAIPMVLTYEEHDYITAGVSHLPHIVAATLVNALASLDTPEEQMKTVA
                     AGGFKDITRIASSSPEVWQQICLSNQKQLSNVLDSYIRLLVQAKYLVDNKKGHELYNM
                     FESSREYRNSMEDSSYGPTKKEYVVYCDLLDEAGGIAAVTTILAINQINIKNIGITHN
                     REFEEGALRIEFYEEQAGILARKVLKDHGYTIHIRN"
     gene            7472..9562
                     /locus_tag="PLN77_05110"
     CDS             7472..9562
                     /locus_tag="PLN77_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRM43691.1"
                     /translation="MDVFRTDRIRNVVLLGHSGSGKTSLTEAMAYLSGMTNRLGKVTD
                     GNTVSDFDKEEIKRKCSISTTLVPVVWGKNKINVLDTPGMFDFVGEAQEAASAADAAV
                     IVVSGKAGVQVGTQKAWELCEKYNLPRMIFVTEMDQDDVSYREVVEQLTELYGKRIAP
                     LHMPLRENGKFVGYINIVKNKGRRYIEKDKKEECPIPDYSVEYLEKYRETLMESVAET
                     SEEFMDRYFGGEEFSVAEISAALAMNVGDGTIVPVCMGSPVNLQGVSNLLDDICGYFP
                     DPSTRPCAGINLNTNEIFEANYDFAKPKSATIFKTIVDPFLGKYSMIKVCSGVIKSDD
                     VLYNVDQESEEKLSKLYVLEGSKPIEVPELHAGDIGAIAKLGDARTGDSLATKNNQVR
                     YGKPDVSTPYTAKRYKPKNKADVDKISQAFAKMMQEDLTMKAVNDSANHQTLLYGMGD
                     QHIDIIVSKLLERYKVEVELSRPKVAFRETIRKNSDVEAKYKKQSGGHGQYGHVKMRF
                     SASGDLEKPYVFEQEVVGGAVPKNYFPAVEKGIQEAVQKGPLAAYPVVGVKAVLYDGS
                     YHPVDSSEMAFKTAAIQAFKKGFMEASPVLLEPIVSMKITVDDKYTGDIMGDLNKRRG
                     RVLGMNPDHKGHTIIEADVPELEIYGYGTTLRSMTGGSGDFSYEFARYEQAPSDIQAK
                     EIEARASKVEKAEE"
     gene            9796..>10881
                     /locus_tag="PLN77_05115"
     CDS             9796..>10881
                     /locus_tag="PLN77_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM43692.1"
                     /translation="MSKRYEPQNIEKKWQKIWQDEKAFAATDDYTKPKYYALVEFPYP
                     SGQGLHVGHPRPYTALDIVARKRRMQGYNVLYPMGWDAFGLPTENYAIKNHIHPKIVT
                     KSNVHHFKDQLQSLGYSFDWDREINTTDPEYYHWTQWIFLKLFKAGLAYKKEMPINWC
                     TSCKVGLANEEVVNGVCERCGAPVVRKVKSEWMLKITDYADKLIEGLNDVDYIERVKV
                     SQKNWIGRSTGAEVDFQIKDTEDKLRIYTTRCDTLFGVTYMVVSPEHPYLDKYKDQIK
                     NWEEIVAYREQASRKSDFERAELAKDKTGVAIDGLTAINPVNGKEVPIWVSDYVLMSY
                     GTGAIMAVPAHDERDWEFAKKFGMPIIE"
BASE COUNT         2874 a         2420 c         2924 g         2663 t
ORIGIN      
        1 gttgggcaaa aaaagttgaa tgaacagagc aaatccggcg aatcatgccg gctgccatag
       61 aatggaggaa acaaatgaaa acatcagatt tttattatga tctgccaaaa gaactgattg
      121 cacaggatcc attggaagac cgcagcgctt cccgcctgat gcatctggat aaaacgacag
      181 gtgaatataa gcatggtcat tttcgtgata ttctgcagta tctgaaacca ggcgactgtc
      241 tggttatcaa tgatacgaag gtaattccgg cgcgtcttta tggttccaag gtcggaacgg
      301 atgcggcaat tgagattctt cttttaaaga gaaaagaaaa tgacatctgg gaaacgctgg
      361 taaaaccggg caaaaaatgc aaggtcggaa ccgtaatatc ctttggagac ggaattctga
      421 caggcgaagt agtcgatatc gtggatgaag gaaaccgcct gatccagttc cactatgaag
      481 gcatttttga agaaattctg gatcagcttg gtgaaatgcc gctgccgcca tatatcacac
      541 ataaactgca ggacaagaac cgttatcaga cggtatacgc aaaacacgaa ggctccgcag
      601 cagctccgac cgcaggactg cactttacaa aagagcttct gcagcaggta caggatgccg
      661 gtgtaaagat cgcccatgta accctgcatg tcggccttgg caccttccgt ccggtgaaag
      721 tggaggatgt cacccagcat cacatgcact ctgagttcta tgtggtagaa gaagatcagg
      781 caaaactgat caacgatacg aaagcagcag gcggccgtgt gatcgcagtc ggaacaacga
      841 gctgccgtac tctggagtcg gcaaccggcg aggacggcat tttaaaagcc ggaagcggct
      901 ggaccgagat ctttatctat ccgggctacc gcttcaagat gatcgacgga ctgatcacca
      961 atttccatct gccggagtcc acactggtga tgctggtatc ggcactggct ggaaaagagc
     1021 atattatggc agcttatgaa gaggctgtaa gagaaaaata ccgctttttc tccttcggcg
     1081 atgccatgat gattgaataa aaaattcgtt tatgacaaaa atacgacgct gaaagaaaac
     1141 gaccatatag gaacaccagc gaagccctat atggtcgttt ttttctctgc gaatcttcta
     1201 aatctttctt tttccctgcc gaaaaacaga agattttatt aaggtttctg aaaacgagcg
     1261 gaactgggtt gtgtcaggga atgtttttgc gtatactgat acgtgtttga gacaagggag
     1321 gacggatttt gaagagagac gagagaagaa agtttccata ctatggtatc ctgccgctaa
     1381 ttctgacgct gacagcgaat tttatcgctt attttggaac gcggccgttt accagttcgt
     1441 ggaaacatta caatatagaa acggtgctgg accagcagat cccggtgatc ccgtggacga
     1501 tcgtgattta ttttggctgt tatctggtct ggattgtaaa ttatctgatt gccgcaagca
     1561 gagagaaaga atttgtctgg cggttctttg cagcagatgt tctggcgcgg cttgtctgta
     1621 tggcatttta tctgctgctg ccgacaacga acgtaaggcc gtcgatcccg gaacagggat
     1681 tctggaatca tatgcttgcc ttgctctacc agatggatgc ggcggacaat ttgttcccat
     1741 cgattcactg cctgaacagc tggttctgct atatcgcagt ccgtggcaga cgggagattc
     1801 cgcgctggta tcagcggttc tctttctggg cggcacttgc cgtgtttgtc tctacactga
     1861 cgacaaagca gcatgtgatt gcagatgtga ttggcggagc gcttctggca gaagtgacgt
     1921 ggcagatcgc gggacgcacg catcttgggg cttggtatgg catgatattg gaacgaagga
     1981 ggtggaaacg ccgtgcagag taagaaaaaa atgatcgtga ataccgtttt tctcgttgcg
     2041 atttttgcgt tgacgatata cggtgttttc cacggcgaag accttggcgc aatgatggaa
     2101 gcaatgcggc gcgcagatgt gcggtggctg ctgccgggac tgctctgtgt cgtgtttttt
     2161 atctggggag aatcgatcat catctggtac atgatgcatt ccttccagat tccggtgaag
     2221 aggcggacgt gttttctgtt ttcttccgtc ggattctttt tcagctgtat cacaccgtcc
     2281 gcaagcggcg gccagccgat gcagctctac tatatgaaaa aggagaaaat atctcttccg
     2341 gtatcaacgg tgattctgat gatcgtcacg atcacctaca aagcggtgct ggtcgtggtg
     2401 ggacttgggc ttgttctgtt cggacagggc ttcctgcacc ggtatctgac ggaaatcctt
     2461 ccggttttct atctgggaat cggtctgaat gtcttctgtg tcacagccat gcttattctg
     2521 gtttttcatc cggcgcttgc gcggacgatt ctggtttttg gactgaaaat cgtggaaaaa
     2581 ctgcatttga tgaaacgaaa agaaagccgc ctgaaaaaac tggaagcttc catggaagtc
     2641 taccagaata cggcagtgta tcttggcacc cacaaaatgg tgctggtcaa tgtccttgcg
     2701 attacctttg cacagaggtt tgctttattt gcagcaacat ggtttgtata ccgcgccttc
     2761 catttatcag gcatttcctt tatcaccatt gtgctgctgc aggcggtcat ttccgtatcg
     2821 gttgacatgc tgccgcttcc gggaggaatg ggaatcagtg agacactgtt cctgaaaatt
     2881 ttcccgccgg tctttggaag cacgctgctt cttccggcaa tggtgctttc cagagggctc
     2941 ggctactacg gggagctgct ggtgagtgca gtctttaccg ttgtcgcaca gcttacgatc
     3001 gggaatacga aaagagaaaa aagaggggga actacatatg atagggttct atgattatac
     3061 cgtaatatta acatatatca gctttgcatc gtccatcagc ggcatttttc ttgcaacgcg
     3121 gggacacttt aactgggcga ttttttgtct tgctttttcc ggactgtgcg atatgttcga
     3181 tggaaaaatt gcgcggacga agaaaaacag gaccgaagat gaaaagcggt tcggcattca
     3241 gatcgattcg ctctgcgatg tggtctgttt cggcgtcttt ccgatcgtgc tctgctatga
     3301 gcttggcatg acacattttt acagcattcc gattcttatt ttctacggtc ttgcgggcgt
     3361 gatccgtctg ggatatttta acgtgatgga agagaagcgt cagaatgaaa cttcggagaa
     3421 ccggaaatat taccagggac tgccgattac ctcgatgtca gtagctctgc cgctgctgtt
     3481 cgtctttgca ccgctctgcc gcggatactt tctgatctgg ctgcatctgc tggtgctggc
     3541 ggtcggcgtt ctgtttatca cggatttccg cttccgcaag ccgacgaata aagagcttgc
     3601 tgtgctggta gcggtcgttg gagtggctgt gctccgcgga cttctgatct ggaagggagg
     3661 aagaacgatt tgagactctg cgcttatcct taccgaaaac aggggaaaaa tcatccaaag
     3721 cttgatctga tccgcgagga aaatgatctg caggaccgtt ttctgcacat gatgtacagc
     3781 catgccgccg gaagagccgc gatgcagccg ctggttcagt catttgtatc tcgtgcggca
     3841 ggctgctttc tggaaagccg cttatcggtg ccgctggttg caccgtttgt aaagaaaaat
     3901 catatttcac tgaaggaatg taccgcaaag cagtttatct cgttcaatga tttctttgtg
     3961 cggaagctga aaatggatgc acgtccgttt tccaatgcgc cgcaggattt tatcagcccg
     4021 tgtgatgcgc ggcttacggt ttatccgatt catgagaacg ggaagtttga gatcaagaac
     4081 acagaatata cgctggaaca gcttctgcgc gaccggaagc tggcaaaacg gtatgagggc
     4141 ggaacgttat ttctgtttcg gctttccgtg gacgattatc accggtatct ctttgtggat
     4201 gacggcgtct gctcgacgga acggcggatt gagggtgtgc tccacacggt caatccggcg
     4261 gcgaatgatt tttgcccgat ctataaagaa aatacgagaa cctacacgct gctgcagtcc
     4321 gcaaatttcg gcacactgct gcagatggag gtcggcgcga tgatggtcgg aaagattgtc
     4381 aatgaaaatg ccggagcggc gagacgtgta cagcgcggcg aagagaaagg ctattttgcc
     4441 ttcggtggtt cgacagtgat cgttctggca cagaagggaa gcgcagtgcc ggaaaaacgg
     4501 atctggaatt attcgcggct tggaatcgaa acgcgggtac ggcagggaga aacggttgga
     4561 acggccggaa gaacagggta aaagcaaaaa aatggattgt gaggaaatga tcttcacaat
     4621 ccattttttg tttaaacgtt ttttatgcct gcagccgtgc cagatcctgg tagaatgtcg
     4681 gatacgagat atccacacag ccggcgcgca ggatttccgt cggtgtctct gcggcaagcg
     4741 aagcgattgc aaacgacatc gcaatgcggt ggtcgaggcg gctgtcgatt tgggcgccgt
     4801 ggaatggttt tccgccctca atgatcatgc cgtcgtccgt gccttccagg gaaacaccca
     4861 tcgcggtgag ttcgtttacc atgatatcaa gacggttcga ttctttgact ttcagctcct
     4921 ccgcattccg gatcacggtt gtaccgttcg ctgtggcggc aagaaccgcg atgatcggga
     4981 tttcatcgat cagggtcggg atcagttcgc cctcgatgac agtgccggaa agagacggcg
     5041 tatagcgcac gagaaggtct gcaaccggtt ctccgccgga gttctgctca ttcagaagtg
     5101 tgatgtcagc acccatggcg cggcagacgc ggagcatacc gtcgcgggtc gggttgatgc
     5161 cgacattttt caggagcacc tcagagcctg gaactatcag cgcggcggcg atgaagtagg
     5221 cggcggagga aatatctccc ggcacacgga cgagatttcc atggagatcc gggtccggca
     5281 gaaccgatgc ggtcagccct tcggaggcaa cgtcggcgcc gaagaatttc agcatcagct
     5341 ccgtatggtt tctggaaaga gccggttcgg taacgcttgt ttttccgtca gcatacagtc
     5401 cggcaagcag gacacacgat ttgacctgtg cggatgcgac cggcgaaaga taggagatgg
     5461 cgtggagcgg atggccgttg atcgcaagcg gcgcacagcc gttttccgga atactgcgga
     5521 tggaagcgcc catctgggtc agcggattga tgatgcgtcc catcggccgt ttctgaatgg
     5581 aagcgtcacc ggtcagtgta ctggaaaagt tctggccggc aagaatgccg gagatcaggc
     5641 gtgtcgttgt tccgctgttt ccgcagtcga gaacggcgga cggagccgaa agcccgtgca
     5701 ggccttttcc atgaacgagg atgcgctccg gcgtgttctc gatctcaatg cccatactgc
     5761 ggaagcagga gatggtggag agacagtcgg caccctgtaa aaagtttgtg acttctgtgg
     5821 ttccctctga aatggcaccg aacatgacgg cgcggtggga aatggattta tcgcccggca
     5881 cggagatttc tccgcgcaga ggacgtactg ctttctggaa ctgcataacg taacttcctc
     5941 ctgtcaagat atacgcgctt ttaattgcgg atatgaatgg tatagccatg gtcttttaag
     6001 accttccggg caagaatgcc tgcctgctct tcataaaatt caatgcggag agcaccctct
     6061 tcgaactcgc ggttatgcgt aatgccgata tttttaatat tgatctggtt gatggcaaga
     6121 attgttgtaa cggctgcgat tccgccggct tcatcgagca ggtcgcagta aacgacgtat
     6181 tctttctttg tcggtccata ggaagaatct tccatggaat tccggtattc gcgggaactt
     6241 tcaaacatat tgtagagctc gtggcctttt ttgttgtcaa ccaggtattt tgcctggacg
     6301 agaaggcgga tataggaatc cagcacattc gagagctgct tctggtttga gagacagatc
     6361 tgctgccata cctccgggga ggaggaagcg atgcgggtga tgtccttgaa gccgcctgca
     6421 gcaacggttt tcatctgctc ttccggtgtg tccagggatg cgagtgcatt gaccagggta
     6481 gcggcaacga tatgcggcag atgactgaca cctgcggtga tgtagtcgtg ctcttcgtat
     6541 gttaaaacca tcgggatcgc ctttaaggac tggacaagat caacgaaagc ggaaactgtc
     6601 gcgatctcaa catctggtcc gggtgtaatc atatagtaag cattttccag caggtaatcg
     6661 gtggcgctgg aatatcccgt cttttcggag cctgccatcg gatggccgcc gataaactgg
     6721 tgcgtcatgc cgagggtctc aaccgcctga tggatttctc cttttacact tccgacgtct
     6781 gtgatgatac agcccggttt tgcgatgcct ttcacgaacg gaagatagga gatgttggtg
     6841 ctgacaggag cacataagaa gatatagtca cagtcggcaa agcggtcgtc ccgttcttcc
     6901 agcgcgatat caatcacgcc ttcctgaatt gcctcttcga tcgtcgcttt ggtgcggcag
     6961 taggccatca gtttatactc cgggtgaacc tttctgaggg tttttgcaat ggagccgccg
     7021 atgaggccaa ggccgataaa gccgatacag attttttcgg atgtatcaca cataattcaa
     7081 gtagtccttt ctgacaattt tcgtaggtaa atcgtataaa agacattgta aagcatctga
     7141 aatcaaatgt caaagatttc ttgctttaaa gtgacaaagt atttttgaaa ggcagcaatt
     7201 ttgcttgttt tgcaaaatcc ggcagattcc acagaaaagc cagacagaaa agcggaagaa
     7261 aggcagaata accagctaaa atacatataa aatttaaaaa ttcagaaata tattgtgaaa
     7321 tatgaataat atacttgcaa aaaagcaaaa actttagtag aatagtgact atgataagag
     7381 gaacggaaga cgtgaatctt gataaaaagc agaaaggcga ataagagcgg ccttgcaatc
     7441 tgcaaattta cttacatggg aggtattaca tatggacgtt tttagaactg accgaatcag
     7501 aaatgtggtg ctgctggggc acagcggctc cggaaagaca agtctgaccg aagctatggc
     7561 ttatctttcc ggtatgacaa accgtctcgg aaaagtgaca gacggaaata ccgtcagcga
     7621 ttttgacaaa gaagaaatca agcgcaaatg ctctatttcc acaacgcttg tcccggtcgt
     7681 atggggaaaa aataagatca atgttctgga tactcctgga atgtttgatt tcgtaggaga
     7741 agcacaggaa gccgcttccg cagcagatgc cgctgtaatc gtggtatccg gaaaggccgg
     7801 cgtgcaggtc ggaacccaga aagcatggga actctgcgag aaatacaatc ttccgcgtat
     7861 gatcttcgta acggaaatgg atcaggacga tgtcagctac cgagaagtcg ttgagcagct
     7921 caccgaactg tacggaaaac gaatcgcacc gcttcatatg cctctgcgtg aaaacggtaa
     7981 atttgtagga tatatcaata ttgtaaagaa caaaggccgc agatatatcg aaaaagataa
     8041 aaaagaagaa tgcccgattc cggattactc cgtagaatat ctggagaagt accgtgaaac
     8101 cctgatggaa tccgtggcag aaacaagtga agagtttatg gaccgctact ttggcggaga
     8161 agagttctcg gtagcagaga tttccgcagc gcttgccatg aacgtaggag acggcaccat
     8221 cgttccggta tgcatgggct ccccggtcaa tctgcagggc gtatcgaacc ttcttgatga
     8281 tatctgcgga tacttcccgg atccgtccac ccgtccgtgt gccggcatca atctcaatac
     8341 gaacgaaatt ttcgaggcaa attacgactt tgcgaaaccg aagagcgcga ccattttcaa
     8401 gacgatcgtg gatccgttcc ttggaaaata ttcaatgatt aaggtttgct ccggcgttat
     8461 taaaagcgat gatgtgctct ataatgtaga tcaggaatcc gaagaaaaac tgagcaagct
     8521 gtacgtgctc gaaggctcca agccgatcga agtaccggag cttcatgcag gagatatcgg
     8581 tgccatcgca aaactcggcg atgcaagaac cggcgattcc cttgcgacaa agaacaatca
     8641 ggtccgctac ggaaaaccgg atgtttcgac tccgtacacc gcaaaacgat ataaaccgaa
     8701 gaacaaagcc gatgtagata agatctccca ggcatttgcc aagatgatgc aggaagacct
     8761 gactatgaag gcagtcaatg attctgcaaa ccatcagaca ttgctgtacg gcatgggcga
     8821 ccagcacatc gatattatcg tcagcaagct gctggagcgc tacaaagtag aagtagagct
     8881 gtcccgtccg aaggtagcct tccgcgagac gatccgcaaa aattcggatg tagaagcaaa
     8941 atacaaaaaa cagtccggcg gccacggcca gtacggtcat gtaaaaatgc gcttctccgc
     9001 ttccggagat ctcgagaaac catacgtttt tgagcaggaa gttgtcggcg gcgccgttcc
     9061 gaagaactac ttcccggctg ttgaaaaagg tatccaggaa gccgtacaga aaggccctct
     9121 ggctgcttat ccggtagtcg gcgtcaaagc cgttctgtat gatggttctt atcatccggt
     9181 agactcctcc gagatggcat tcaagaccgc agcgatccag gcgttcaaga aaggctttat
     9241 ggaagcatcc ccggttctcc ttgagccgat cgtttcgatg aagatcactg tagacgataa
     9301 atacaccggc gatatcatgg gcgatctcaa caaacgccgc ggccgtgtgc tcggcatgaa
     9361 cccggatcac aagggccata cgattatcga ggctgatgtt ccggaacttg agatctacgg
     9421 atacggcaca acactccgct ccatgacagg cggaagcggt gatttctcct atgagtttgc
     9481 gagatatgag caggctccgt ccgatatcca ggcaaaagag attgaggcaa gagcttcgaa
     9541 ggttgaaaaa gcagaagaat aaagctttat aaaatagtgt gataaaacag agaaaatccg
     9601 gtttcgggag cagaattcgc ccggaaaccg gatttttctg ctgtttttga agaaaaacgg
     9661 aggatatagg aaaaaataaa ttgtaaaaac agggaaagag tgatagaata cagctatatg
     9721 ttcaaatgtg catgctgtcc tatgcggcag tgtgagaaaa aagaactttg aacttgaaca
     9781 tgcaggaggt tacccatgag taaaagatat gagccacaga acattgagaa aaagtggcag
     9841 aagatctggc aggatgaaaa agcctttgcc gccacagatg attatacaaa accaaaatac
     9901 tatgcactcg ttgagttccc gtatccgtca ggacagggac ttcacgtagg ccatccgcgt
     9961 ccgtacaccg cactggatat cgtagccaga aagcgcagaa tgcagggcta caacgtactg
    10021 tatccgatgg gatgggatgc atttggtctt ccgactgaga actatgcgat caagaaccac
    10081 atccatccga agatcgttac caaaagcaac gtacatcact tcaaagatca gcttcagtcc
    10141 cttggatatt cctttgactg ggacagagag atcaacacca ccgatccgga gtactatcac
    10201 tggacccagt ggatcttcct caaactcttc aaagcaggtc ttgcttacaa gaaagaaatg
    10261 ccgatcaact ggtgtacttc ctgtaaggta ggtcttgcaa acgaggaagt tgtaaacggt
    10321 gtatgtgagc ggtgtggcgc tccggttgtc cgcaaggtaa agagcgagtg gatgttaaag
    10381 atcacagatt atgcggataa gctgatcgaa ggactgaatg atgtagacta cattgaacgt
    10441 gtcaaggttt cacagaaaaa ctggatcgga cgttctacag gtgccgaggt tgattttcag
    10501 atcaaagata cagaagacaa gctgcgtatt tacaccacac gctgcgatac attgtttggt
    10561 gttacctata tggttgtttc accagagcat ccatatctgg ataagtacaa ggatcagatt
    10621 aaaaactggg aggaaatcgt tgcataccgc gagcaggctt ccagaaagtc tgattttgag
    10681 cgggcggagc ttgcaaaaga caagactggt gtggcaattg acggtctgac tgcaatcaat
    10741 ccggtcaacg gaaaagaagt tccgatctgg gtatctgatt atgtactgat gagctatggt
    10801 accggtgcta tcatggcagt tccggcacat gatgagagag actgggagtt cgctaaaaag
    10861 tttggtatgc ctattatcga a
//
LOCUS       DAOMUF010000062        10840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1465_length_10840_cov_2.990542, whole genome shotgun sequence.
ACCESSION   DAOMUF010000062 DAOMUF010000000
VERSION     DAOMUF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_10840_cov_2.990542"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLN77_05120"
     CDS             complement(<1..767)
                     /locus_tag="PLN77_05120"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRM43693.1"
                     /translation="MAKISIIGAGSWGTALGLLLCNNGHDVTICSENEKEIESLKEHR
                     EHLTKLPGVKLPDEMKFTCDVRGSLSGQDILVMAKASPYVRTTCKKYAADIPDGQLIV
                     NVAKGVEADTLMTMSQIIEEEVPGANVVVLSGPSHAEEVGRGLPTTIVVGAHDRESAL
                     YVQNVFMSPVFRVYTSPDITGIELGGALKNVIALAAGTADGLGYGDNTKAALITRGIA
                     EIARLGTAMGAHAETFYGLSGIGDLIVTHLYLSFGNG"
     gene            complement(771..1418)
                     /gene="plsY"
                     /locus_tag="PLN77_05125"
     CDS             complement(771..1418)
                     /gene="plsY"
                     /locus_tag="PLN77_05125"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRM43694.1"
                     /translation="MVRIICLLIGYAFGLFQTSYIYGRTKGIDIREHGSGNAGTTNAL
                     RTLGKKAGAITLAGDCIKCILAVLTVRLIFGASHPEMLKLLTVYAAAGTILGHNFPLY
                     LGFRGGKGIAATAGLIISFDWRITLVCAALFFLNLAVTHYVSLGSMMVYVVFFAMAVI
                     MGQAGCFALEPPYLYEMYVVIALLAVLAFWEHRANIKRLIAGTERKTYLSKKSEK"
     gene            complement(1425..2753)
                     /gene="der"
                     /locus_tag="PLN77_05130"
     CDS             complement(1425..2753)
                     /gene="der"
                     /locus_tag="PLN77_05130"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRM43695.1"
                     /translation="MSKPIVAIVGRPNVGKSTLFNVLAGDNISIVKDTPGVTRDRIYA
                     DVEWLNHKFTLVDTGGIEPESKDIILSQMRDQAQIAIDTANVIIFITDVRQGLVDADA
                     KVADMLRRSRKPVVLVVNKVDNFDRQMMDVYEFYNLGIGEPHAISASGKLGIGDMLDE
                     VTGYFDPEMENEEESEIPRIAIVGKPNVGKSSLVNKLLGSNRVIVSDIAGTTRDAIDT
                     TIMHNGNEYIFIDTAGLRKKNKIKEELERYSIIRTVSAVERADVVVLLIDATEGVTEQ
                     DAKIAGIAHERGKGMIIAVNKWDALEKDDKTIYRFTEKVRNTLAYMPYAELLFISAKT
                     GQRLPKLFETIDMVLQYQNLRVQTGVLNEILTEAMAMQQPPSDRGKRLKIYYMTQVSV
                     KPPTFVIFVNDKELMHFSYVRYLENKIRESFGFRGTPLKFIIRERKEKEQ"
     gene            complement(2707..4101)
                     /locus_tag="PLN77_05135"
     CDS             complement(2707..4101)
                     /locus_tag="PLN77_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HRM43696.1"
                     /translation="MKNKTKHIIKTIEPGSIAEELELEPGDELLRVNGEIIEDVFDYH
                     FQTNDTYLELTIRKKDGEEWDLEIEKEYDEEIGITFENEFMDQYRSCSNNCIFCFIDQ
                     MPPGMRPTLYFKDDDSRLSFLQGNYITLTNMSDHDIDRIIRYHLAPINISFQTTNPEL
                     RCKMLHNRFAGNIFPKVQRLYEAGIEMNGQIVLCKGYNDGAELERSISDLSKYLPHLK
                     SVSVVPVGLSKYRDGLAPLEPFTREDAKEVLATIHKWQKKLYEQWGLHFIHAGDEWYL
                     LAGEPIPEEENYDGYIQLENGVGMLRLLEDEVAEELSKREGDDRHRHVTIATGKAAAP
                     SLKKHMQKIREKYPNVQAEVVTIINYFFGESITVSGLITGKDLMEQLEGRDLGDCLLL
                     PCNMLRSGKNVFLDDVTVEEVEERLKTPVQIIDEPGSDLVKAVVEENQETIHRRRTMY
                     EQADCSNCGPAERG"
     gene            4325..5488
                     /locus_tag="PLN77_05140"
     CDS             4325..5488
                     /locus_tag="PLN77_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRM43697.1"
                     /translation="MANLDLLENSIPVAPLKLAALPGSMEMAKKVDSYLVQFRKELAE
                     RRNGVSFSGYSEDSFLIDCECPRFGSGEAKGKINESVRGADLYILVDVCNYSITYKVC
                     GYENHMSPDDHYQDLKRIIAAATGKAHRINVIMPFIYEGRQHKRTKRESLDCALALQE
                     LMNMGVSNFITFDAHDPRVMNAIPLGGFDNFMPTYQFLKALLRSVPDLTIDNDHLMII
                     SPDEGAMARAVYFSNVLGVDMGMFYKRRDYSTIVNGKNPIVAHEFLGDSVAGKDVVII
                     DDMISSGESMLDVAKQVKERGADHVFVCTTFGLFTDGLEKFDAYYEKGYISKVITTDL
                     TYRTPELVSRPWYEPAEMSKYMASIIDTLNHDVSVAKVQSTTEKINKLLAKHR"
     gene            complement(5586..6440)
                     /gene="pgeF"
                     /locus_tag="PLN77_05145"
     CDS             complement(5586..6440)
                     /gene="pgeF"
                     /locus_tag="PLN77_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRM43698.1"
                     /translation="MIPIQWKQNDKKTMHVNEAENGVVYLTWPAIEKIEGIRHAFSTR
                     IGGVSKEHLSSMNLSFSRGDDPANVRENYRRFCEAAGFEVENIVTSDQAHTTKVRYVT
                     KADCGSGVTRDRDFHDIDGMITDEPGVVLATFYADCVPLYFVDPVHRAIGLSHSGWRG
                     TVHKMGQATLDAMHERFGTEAKDVIAAVGPSICQDCYEVSGDVIEEFRAAFPETLHEK
                     LFYGKPDGKYQLNLWEANHQILLAAGVPEKQIHLPNLCTCCNPDFLYSHRASKGKRGN
                     LAAFLSLV"
     gene            complement(6454..6816)
                     /locus_tag="PLN77_05150"
     CDS             complement(6454..6816)
                     /locus_tag="PLN77_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRM43699.1"
                     /translation="MNQREVGARQESRAAEYLESLGYHILVRNFRCRFGEIDLIAKQD
                     DTYIFIEVKYRTGRTAGDPTSAVDGKKQKKISKTADYYRMLHQIPMDVPCRFDVVAET
                     PEEIRVYANAFYYQGSGW"
     gene            complement(6852..7619)
                     /locus_tag="PLN77_05155"
     CDS             complement(6852..7619)
                     /locus_tag="PLN77_05155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRM43700.1"
                     /translation="MAEKKQSISAIREIFAAASETELPALYLEYEEDSRAGVQNLIQK
                     YQKQEEALKKERERTEQMKIYEHKYEDLGWICGIDEVGRGPLAGPVVAGAVILPRDSK
                     ILYLNDSKQLTAKKRDELYDVIMREAVAVGIGYASPARIDEINILQATYEAMREAISK
                     LSVKPDVLLNDAVKIPQVDIRQVPIIKGDAKSVSIAAASIVAKVTRDRLMEEYDKVLP
                     GYGFASNKGYGSAEHIAALKEIGPSPIHRQSFIGHFV"
     gene            complement(7656..8498)
                     /gene="ylqF"
                     /locus_tag="PLN77_05160"
     CDS             complement(7656..8498)
                     /gene="ylqF"
                     /locus_tag="PLN77_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HRM43701.1"
                     /translation="MNYQWYPGHMTKARRQMQEDLKLIDLMIELVDARVPAASRNPDI
                     DELGKNKARLILLNKSDLADERGNDAWMEYFKGKGYSVVKVNSRSGAGMKAINSAVQE
                     ACKEKIERDRKRGILNRPVRAMVVGIPNVGKSTFINAYAGRACAKTGNKPGVTKGKQW
                     IRLSKQLELLDTPGILWPKFEDQQVGLHLAMIGSINDEILNVEELALSVIDYLTAEYP
                     GTLEKRFGFEGMTDRVEILGEIARVRGCLKKGNELDYEKAARLLMDDFRSGKLGRITL
                     EKPE"
     gene            complement(8523..9080)
                     /gene="lepB"
                     /locus_tag="PLN77_05165"
     CDS             complement(8523..9080)
                     /gene="lepB"
                     /locus_tag="PLN77_05165"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM43702.1"
                     /translation="MNTRYVKEYFYKKKIRSMFVWNLEIIAAILLAAAFSWFFCRSVV
                     VQEGSMEPTLVPGERVLMDSAWYRLSSPKRGDIIAFRTSEDKKASIHIKRVIALPGET
                     VQIKDGQILINGEVYNEKDEFPAITNAGLASEPITVGKGQYFVLGDNRNNSEDRHNGM
                     GLVDSDRIVGKLWFTISPWKKIGFV"
     gene            complement(9162..9509)
                     /gene="rplS"
                     /locus_tag="PLN77_05170"
     CDS             complement(9162..9509)
                     /gene="rplS"
                     /locus_tag="PLN77_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRM43703.1"
                     /translation="MNDIIKKIEDAQLKAEAPEFRVGDTVKVYGKIKEGNRERIQVFE
                     GVVLKRQGGGSKETFTVRKSSNGVGVEKTWPVHSPNVEKVEVIRRGKVRRAKLNYLRD
                     RVGKAAKVKELVK"
     gene            complement(9598..10722)
                     /gene="trmD"
                     /locus_tag="PLN77_05175"
     CDS             complement(9598..10722)
                     /gene="trmD"
                     /locus_tag="PLN77_05175"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRM43704.1"
                     /translation="MKYHILTLFPEMIEQGLHTSILGRAINNGYISLETTNIRDFSAN
                     KFNRVDDYPYGGGAGMVMEAEPVFRAYQSVAEKIGKKPRTVYLTPQGKVLNQTMVEEL
                     ALEDDLVLLCGHYEGIDDRVLQEVVTDYISIGDYVLTGGELGAMVLVDAVSRFVPGVL
                     SNEESSQFESLQDNLLEYPHYTRPETWHEKKVPEVLLSGDHKKIEAWRHEASLVRTAE
                     RRPDLLENAFQISCACNEKEESSAWAHDLLAGMTRYGVSLDLGRKKIRKQKNLFDDHD
                     LLILQLPGTLEEGMKAKSEYIRSFAGKETPLVFLCPDGFSEEEEKLEEQLEKNGFRLV
                     ARLTGIPSADGLQRFSFALRSLLYSGEWKVKKILASADAL"
     gene            complement(10741..>10840)
                     /locus_tag="PLN77_05180"
     CDS             complement(10741..>10840)
                     /locus_tag="PLN77_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HRM43705.1"
                     /translation="GEVLIPAIHDCILDVDLDEQKMTIHLLPGLID"
BASE COUNT         2346 a         3038 c         2499 g         2957 t
ORIGIN      
        1 taaccgtttc caaaactgag atagaggtgt gttacaatca ggtcaccgat gccggaaaga
       61 ccatagaagg tttccgcatg ggcacccatc gccgtgccga gccgtgcaat ttccgcgatc
      121 ccgcgggtaa tcagagccgc tttcgtattg tcgccgtagc caagaccatc cgccgttccg
      181 gctgccagtg cgatgacatt tttcagagct ccgccaagtt caattcccgt gatgtccggg
      241 cttgtgtaaa cgcggaagac cggactcatg aacacattct gcacatacag tgcgctctcg
      301 cggtcatgcg cgccgacaac gatcgtggtc ggcaggccgc ggccgacttc ctctgcgtgg
      361 ctcggtccgg agagaacaac gacattcgct cccggcactt cctcctcgat gatctgagac
      421 atcgtcatca gggtatcggc ttccacgcct tttgccacat tgacgatgag ctggccgtcc
      481 ggaatatccg ccgcatactt tttacatgtg gttcgcacgt acggggatgc ttttgccatc
      541 acaaggatgt cctgtccgga aagggagccg cgcacatcgc aggtgaactt catttcatcc
      601 ggcagtttta cgcccggaag cttcgtcaga tgctctctgt gttcctttaa gctctcaatt
      661 tccttttcat tttccgagca gatcgtcacg tcatgcccgt tgttgcagag caggaggccg
      721 agggcggttc cccagcttcc tgctccgatg atgctaatct ttgccatatt ttatttctcc
      781 gactttttgg ataaatacgt tttccgctct gttcccgcga tgagacgctt gatgtttgca
      841 cggtgctccc agaaggcaag cacggcaaga agtgcgatca ccacgtacat ctcatagaga
      901 tacggcggtt ccagcgcaaa gcagcccgcc tgtcccatga taacggccat tgcaaaaaag
      961 acaacgtaaa ccatcatgga accgagggaa acatagtgtg ttactgcaag attcaggaaa
     1021 aacagcgctg cacagaccag cgtgattctc cagtcaaacg aaataatcag acccgcggtc
     1081 gcagcgattc cttttccgcc gcggaagcct aagtaaagcg ggaagttgtg gccgagaatc
     1141 gttccggccg cagcatacac cgtcagcagt ttcagcattt ccgggtgcga tgcaccgaag
     1201 atcaggcgta ccgtcaggac cgccagaatg catttgatgc agtcgccggc aagcgtgatg
     1261 gcgcctgctt ttttccctaa agtccggagc gcgttggtcg ttcctgcgtt tccgctgccg
     1321 tgttcccgga tatcaattcc cttcgtcctt ccataaatat atgaggtctg aaaaaggcca
     1381 aatgcgtatc cgatcaaaag gcatatgatc cgtaccataa ctctttactg ctccttctct
     1441 tttcgttctc tgatgatgaa tttcagcggt gtgccgcgga agccgaacga ctctctgatt
     1501 ttattctcca gatatcgaac gtaggagaaa tgcatcagtt ctttgtcgtt gacgaaaata
     1561 acaaatgtcg gcggtttgac cgaaacctgt gtcatgtagt agattttcag tcgttttcca
     1621 cggtctgacg gcggctgctg cattgccatt gcctcggtca gaatctcgtt caggacaccg
     1681 gtctgtacgc gcaggttctg gtactgcaga accatgtcga tcgtctcgaa cagcttcgga
     1741 agacgctgtc ctgtcttcgc agagatgaaa agaagctctg cgtacggcat gtaagccagt
     1801 gtatttctta ctttttcggt aaaacggtag atcgtcttgt catctttttc caaggcatcc
     1861 catttattta ccgcaatgat catacctttt ccacgctcat gggcgatacc ggcaattttg
     1921 gcatcctgct cggtgacacc ctcggttgca tcgatcagaa gaacgacgac atctgcgcgc
     1981 tctacggcag atactgtacg gatgatgctg taccgctcca gctcctcttt gatcttgttt
     2041 ttctttcgaa gacctgcggt atcgatgaaa atatattcat ttccattgtg catgatcgtg
     2101 gtatcgatcg catcgcgcgt cgttccggcg atatcggata cgatgacacg gttcgagccc
     2161 agcagtttgt tgaccaggga ggattttccg acgttcggtt ttccgacaat ggcgatgcgc
     2221 ggaatctcgc tctcctcctc gttttccatc tccggatcaa agtatcctgt cacctcatcg
     2281 agcatatcgc cgatgccgag ctttccggat gcggaaatgg cgtgcggctc tccgatgccg
     2341 aggttataga attcataaac atccatcatc tgacggtcga agttgtcgac tttgttgacc
     2401 accagcacaa ccggcttgcg gcttcttctg agcatatcag ctacttttgc atcggcatct
     2461 acaaggccct gacggacgtc agtgatgaag atgatgacat ttgcggtatc aatggcgatc
     2521 tgcgcctggt ctctcatctg ggagagaatg atatctttgg attccggctc aataccgccg
     2581 gtatctacca gcgtaaattt gtggttcagc cactccacgt cggcataaat acggtcacgg
     2641 gttacacccg gtgtgtcttt tacaatcgaa atattgtctc cggctaatac attaaacagg
     2701 gtggatttac ccacgttcgg ccggcccaca attgctacaa tcggcttgct catacattgt
     2761 tctccttctg tgtatcgtct cctggttttc ctccacaact gctttcacca ggtcgctgcc
     2821 cggctcatcg ataatctgta ccggtgtttt caggcgctct tccacctctt ccaccgtcac
     2881 atcgtcgaga aagacgtttt ttcccgaacg gagcatgttg cacggaagca gcaggcagtc
     2941 gccaagatct cttccctcta actgctccat cagatccttt ccggtaatca gaccggatac
     3001 ggtaatggat tctccaaaga aatagttgat aatcgtgaca acctccgcct gtacattcgg
     3061 atatttttca cgaattttct gcatgtgttt tttcaacgac ggcgccgcag cttttccggt
     3121 cgcaatcgtt acatggcggt gacggtcgtc cccttctcgt ttcgaaagtt cttccgcaac
     3181 ctcgtcctcc agaagccgga gcattcccac tccgttttcc agctggatat agccatcgta
     3241 attttcctcc tccgggatcg gctctcccgc cagcagatac cattcatcac cagcatggat
     3301 aaagtgaagt ccccactgtt catacagctt tttctgccat ttgtgaatcg tggcaagaac
     3361 ttcttttgca tcctccctgg taaacggttc cagaggcgcc agtccgtcgc ggtacttcga
     3421 aagtccgacc gggacaaccg aaacactttt cagatgcggc agatattttg acagatccga
     3481 aatactgcgc tccagctctg cgccgtcgtt gtatcccttg cagagcacaa tctgtccatt
     3541 catctcgatt cccgcctcgt aaaggcgctg gaccttcgga aagatattgc ccgcaaaacg
     3601 gttgtgcagc attttgcagc ggagctccgg gttcgttgtc tggaacgaaa tattgatcgg
     3661 tgccagatgg tagcggataa tccggtcgat atcgtggtcg ctcatgttgg tcagtgtaat
     3721 atagtttccc tgcaggaagg acagacgcga atcgtcatcc ttaaaataca aggtgggacg
     3781 cattcccggc ggcatctgat cgatgaagca gaaaatgcag ttgttgctgc aggaacggta
     3841 ctgatccatg aactcgttct cgaatgtgat gccgatttcc tcgtcatact ctttttcgat
     3901 ttccaggtcc cattcctcgc cgtccttttt ccggatcgtg agttccagat acgtgtcgtt
     3961 ggtctggaag tggtaatcga agacgtcctc gatgatttcc ccgttcacgc ggagcagttc
     4021 atctcccggt tcgagttcca gttcttcggc aatactgccc ggttctattg tcttgataat
     4081 atgtttcgtc ttgtttttca cagatcttac gttttggcct ttccatttac ttctttataa
     4141 cttctctttg cccagtaaaa agcgtgcttt ttttgcatac cttccggaca ttcatccatt
     4201 ataataacaa caacctgcac aaaaagcaag gattctttcg ttttttgctt gacgcgccct
     4261 ttcccaagat gatataaata ggaagtaaat ggtttgtaaa aaatgtcgat tactggaggt
     4321 ttgtatggct aatttagatt tacttgaaaa ttccattccg gttgctccgt taaagctcgc
     4381 agctctgccg ggcagtatgg aaatggcaaa aaaagttgat tcgtatctgg ttcagttccg
     4441 taaagagctc gccgagcgcc gcaacggggt ttccttttcc ggatattccg aggattcttt
     4501 tctgattgac tgcgagtgcc cgcgtttcgg ttccggcgaa gcaaaaggaa agatcaacga
     4561 atccgtccgc ggtgccgatc tttacatcct ggttgatgtc tgcaactaca gcatcactta
     4621 caaggtctgc ggctacgaga accacatgtc accggatgac cattatcagg atctgaaacg
     4681 tatcatcgct gctgcaacgg gaaaagcaca ccggatcaac gtaatcatgc ctttcattta
     4741 tgaaggaaga cagcataagc gcaccaaacg tgagtctctc gactgcgcac ttgctctgca
     4801 ggagctgatg aacatgggtg tttccaactt catcaccttc gacgcacacg atcctcgtgt
     4861 catgaacgca attccgctcg gcggctttga caacttcatg ccaacctacc agtttttaaa
     4921 agcccttctt cgttccgttc cggatctgac gatcgacaac gatcatctga tgatcatcag
     4981 cccggacgag ggagcgatgg cgcgtgccgt atacttctcc aacgtgctcg gcgtcgatat
     5041 gggtatgttc tataagagaa gagattactc caccatcgta aacggaaaga acccgatcgt
     5101 ggcacatgag ttcctcggcg attccgtagc aggaaaagat gttgtcatca tcgacgatat
     5161 gatctcctcc ggtgagagta tgcttgacgt tgcaaaacag gtcaaagagc gcggcgctga
     5221 ccacgtcttt gtctgcacca ccttcggcct cttcaccgat ggtctggaga aattcgatgc
     5281 ttactatgag aaaggctata tcagcaaagt catcaccacc gacctgacct accgtacacc
     5341 ggaactcgtt tcccgtcctt ggtatgagcc ggctgagatg agcaaatata tggcaagcat
     5401 catcgatacg cttaaccacg acgtatccgt tgcaaaagta cagtctacga cagagaaaat
     5461 caataagctg ctggcaaaac acagatagtc gattttgtgt ccgccggatg catacagcaa
     5521 aaaaacggat ctgttttttc caggtccgtt ttttgatgct ttctattctg ttttcgatcc
     5581 tgttttcaga ccagggaaag gaatgccgcg aggtttcctc tttttccctt cgacgcacgg
     5641 tgggaataca ggaaatccgg gttgcagcag gtgcagaggt tcggcagatg aatctgcttc
     5701 tccggcactc ctgccgcaag aaggatctga tgattcgcct cccagagatt taactggtat
     5761 tttccatctg gttttccgta gaacagtttc tcatgcagtg tttccgggaa tgctgcccgg
     5821 aactcttcga tcacgtcacc gctcacctcg tagcagtcct ggcagatcga cgggccgacg
     5881 gctgcgatca catcttttgc ttctgttcca aaccgttcgt gcatcgcatc gagcgttgcc
     5941 tgtcccatct tgtggaccgt tccacgccag ccggaatgtg aaagtccaat tgcacggtgc
     6001 accgggtcga caaagtagag cggcacgcag tccgcataga atgtcgcaag tacgacgccc
     6061 ggctcgtcag tgatcatacc gtcaatatca tgaaaatcgc ggtctcttgt cacgccgctg
     6121 ccgcagtctg ctttcgtcac ataacggacc ttcgttgtgt gtgcctgatc ggaggtaaca
     6181 atgttttcca cttcaaaccc cgccgcctca cagaatcttc tgtaattctc ccgcacattc
     6241 gccggatcgt cgccgcgcga aaagctgaga ttcatgctgg aaagatgttc cttactcacc
     6301 ccgccgatcc gtgtggaaaa tgcatggcgg atgccctcaa ttttctcaat cgccggccag
     6361 gtcagataga ccacgccgtt ttccgcctcg ttgacatgca tcgttttttt atcgttttgt
     6421 ttccattgaa ttggtatcat aaaaactccc ttcctaccag ccgctcccct gataataaaa
     6481 cgcgttcgca tagacgcgga tctcctccgg tgtctctgcc acgacatcga agcggcaggg
     6541 cacatccatc gggatctggt ggagcatgcg gtagtaatct gctgtttttg aaattttctt
     6601 ctgtttcttt ccatccacgg cgcttgtcgg gtctccggct gtccggccgg tgcggtattt
     6661 tacttcgata aaaatgtatg tgtcgtcctg ttttgcgatc aggtcgatct caccgaagcg
     6721 gcaccggaaa ttccgcacca ggatgtgata tcccaggctt tccagatatt ctgccgcacg
     6781 gctctcctgt ctcgctccta cttcccgctg gttcaaatgc gataccctct cctttttttg
     6841 atgcggtgcc tttacacgaa atggccgata aacgactgcc ggtggatcgg tgacgggccg
     6901 atttctttta aagcagcgat atgctctgcc gaaccgtagc ccttgttcga tgcaaaaccg
     6961 tagcccggaa gcaccttgtc gtactcttcc atcaggcggt cccttgtcac cttcgcaaca
     7021 atacttgccg ccgcaatcga aacactcttg gcatctccct tgatgatcgg cacctggcgg
     7081 atgtccacct gcgggatttt caccgcatcg tttaagagca catccggctt cacggaaagc
     7141 ttgctgatcg cctcccgcat cgcctcgtac gttgcctgta aaatattgat ctcgtcgatc
     7201 cgcgccgggg aggcatagcc gatgccgacg gccactgcct cgcgcatgat cacatcgtac
     7261 agctcgtcgc gttttttggc cgtcagctgc ttggaatcgt tcagatacag gattttactg
     7321 tcgcgcggca gaatgaccgc acctgcgacc acagggcccg ccagagggcc tcttccgacc
     7381 tcgtcgatgc cgcagatcca gccaagatcc tcatatttgt gctcgtagat cttcatctgt
     7441 tccgtgcgct cacgctcttt ttttaaggcc tcttcctgtt tctggtattt ttgaatcaga
     7501 ttttgtacgc cggcacggga atcctcttca tattccagat aaagggcggg cagctccgtc
     7561 tccgaagctg ccgcaaaaat ttcccgtatg gcggatatcg attgtttttt ctctgccatc
     7621 gttgttctcc gcatttttta ttttgataaa tccatttatt ccggtttttc cagcgtgatt
     7681 ctgccgagct ttccgctccg gaaatcatcc atcagaagcc gtgctgcttt ttcataatcg
     7741 agctcatttc cctttttcag gcagccgcgc acacgcgcaa tctcacccag gatttccaca
     7801 cgatccgtca taccttcaaa gccaaagcgt ttctccagcg ttcccggata ctctgccgtc
     7861 agataatcga tgacggacag cgccagctcc tccacgttca ggatctcatc gttgatggaa
     7921 ccgatcatcg caagatgcag tcccacctgc tggtcctcaa acttcggcca caggatacca
     7981 ggtgtgtcca gaagctccag ctgtttgctc agacggatcc attgttttcc cttcgtgacg
     8041 cccggtttgt ttccggtttt cgcacaggct ctgcccgcat acgcattgat aaatgtcgat
     8101 tttccgacgt tcggaatacc gacaaccatc gcgcggaccg gacggttcag gatgccgcgt
     8161 ttgcggtcgc gctcgatttt ctctttgcag gcctcctgta cggcgctgtt gatcgctttc
     8221 atgccggctc cggaacggga attgaccttc acaacagaat atccctttcc cttgaaatat
     8281 tccatccagg catcgtttcc gcgctcatcg gcaaggtccg atttgttcag caggatcagc
     8341 cgtgctttgt tctttccaag ttcatctata tccgggttgc ggctcgctgc aggaactctc
     8401 gcatcgacca gctcaatcat caggtcgatg agctttaagt cttcctgcat ctgtctgcgg
     8461 gccttcgtca tatgacccgg gtaccactga taattcatgt ttcttctcct tctatgctgc
     8521 gtttacacga aaccgatttt cttccacggc gaaatcgtaa accagagctt tccgacaatg
     8581 cggtccgaat ccacgagtcc catgccgttg tggcggtcct cgctgttgtt ccggttgtcg
     8641 ccgagcacaa agtactgtcc cttcccgacc gtgatcggct cggaggcaag tcccgcgttt
     8701 gtgatggccg gaaattcatc tttttcatta tagacttccc cgttgatgag gatctgtccg
     8761 tctttgatct gtaccgtctc gcccggcagc gcaatcacac gcttgatgtg gatgctcgcc
     8821 ttcttgtcct cactcgtccg gaacgcgatg atatccccgc gtttcggaga acttaagcgg
     8881 taccaggcgg agtccatcag cacacgctcc cccggcacga gagtcggctc catcgagccc
     8941 tcctgcacca cgacacttct gcagaaaaac caggaaaatg ccgccgcaag aagaatcgct
     9001 gcgatgattt ccagattcca tacaaacatg gaacggatct ttttcttgta gaaatattct
     9061 tttacatatc tggtattcat acccatagtg taattctccg taagacaaaa ccggagaaac
     9121 acaattgctc atgttcctcc ggccatcctt ttaacgactt attattttac cagctctttc
     9181 actttcgctg ctttaccaac acggtctctt aagtagttca gtttcgcacg tcttacttta
     9241 ccacgtctga taacttctac tttctcaacg ttcggagagt gtaccggcca tgttttctca
     9301 acgcctacgc cgttagagct cttacgaaca gtgaaggtct ctttgcttcc gccgccctgt
     9361 cttttcagga caacgccttc aaatacctgg atacgctcgc ggttaccctc tttgatttta
     9421 ccgtaaactt taacggtatc accaacgcgg aactccggtg cttctgcttt taactgtgca
     9481 tcttcgattt tcttaataat gtcgttcatt tctttatctc ctttttttac cctggatgtt
     9541 cttaatgcct ttttgcaaca gcggaccatc tctttactca caacaagata tattatacta
     9601 taatgcatcc gcagatgcaa gtattttttt cactttccat tcgccgctgt acagcagact
     9661 gcgaagcgcg aaggaaaacc gctgcaggcc gtccgcggac ggtatgccgg taagccgtgc
     9721 gaccagacgg aaaccgtttt tttccagctg ctcttcgagt ttttcttcct cttccgagaa
     9781 tccgtccggg cataaaaaca ccagcggtgt ctcttttccg gcaaagctgc ggatatattc
     9841 gctctttgct ttcataccct cctcaagcgt tcccggaagc tgcagaatca gcagatcatg
     9901 gtcatcaaac agatttttct gtttccggat cttctttcgt ccgagatcaa ggcttacacc
     9961 atagcgtgtc atgcctgcca aaagatcatg cgcccaggca gacgattcct ctttttcatt
    10021 gcatgcgcag gaaatctgga atgcattttc cagaaggtcc ggccgccgct cggctgtccg
    10081 caccagagag gcctcatggc gccatgcttc gatctttttg tggtcaccgg aaagaagcac
    10141 ctccggaact tttttctcgt gccatgtctc cggtcttgtg taatgcggat actcaagcaa
    10201 gttgtcctgc agtgactcaa actgcgaaga ttcctcgttg cttaagacac ccggcacaaa
    10261 ccgcgatacc gcatccacca gcaccattgc accgagttct ccgcctgtta aaacataatc
    10321 cccgatcgag atatagtccg ttaccacttc ctgcagtacg cggtcatcga tgccctcata
    10381 gtggccgcag agcagcacaa gatcgtcctc aagcgccagc tcttccacca tcgtctggtt
    10441 cagtactttt ccctgcggtg tcagatagac cgtgcgcggc tttttcccga tcttttcggc
    10501 taccgactgg taggcgcgga acaccggctc cgcctccatg accatgcccg caccgccgcc
    10561 gtacgggtaa tcgtccacgc ggttgaattt atttgctgaa aaatcccgga tattcgtcgt
    10621 ctcgagggaa atatagccgt tgttgatggc tcttccgagg atgctggtgt gcagcccctg
    10681 ctcgatcatc tccggaaaca gcgtcagaat atgatatttc atcgttcttt actctcttta
    10741 tcagtcaatc aggcccggaa gaaggtgaat cgtcatcttc tgctcatcca ggtccacatc
    10801 cagaatgcag tcatggatcg ccggaatcag aacctctccg
//
LOCUS       DAOMUF010000063        10761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1483_length_10761_cov_4.672800, whole genome shotgun sequence.
ACCESSION   DAOMUF010000063 DAOMUF010000000
VERSION     DAOMUF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_10761_cov_4.672800"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..773)
                     /locus_tag="PLN77_05185"
     CDS             complement(228..773)
                     /locus_tag="PLN77_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HRM43706.1"
                     /translation="MKKFVCSVCGYVYEGAEAPEVCPICKAPKEKFIEQGEDLTWAAE
                     HVVGVASDVPEDIKKDLRANFEGECSEVGMYLAMSRVAFREGYPEVGLYYEKAAYEEA
                     EHAAKFAELLGEVVTDSTKKNLQMRVEAENGATAGKTDLAKRAKALNLDAIHDTVHEM
                     ARDEARHGKAFAGLLARYFGK"
     gene            complement(944..1342)
                     /locus_tag="PLN77_05190"
     CDS             complement(944..1342)
                     /locus_tag="PLN77_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRM43707.1"
                     /translation="MSTLKYSRQRESIKVCLQGRKDHPTADMLYTDVRKEFPNISLGT
                     VYRNLSLLSELGEIKRLTTGDGPDRFDGDTSTHHHFICRKCHCVTDLTMRDISEIKNV
                     AAENFRGTIEDYSVNFYGICENCKNHPGNV"
     gene            1856..3007
                     /locus_tag="PLN77_05195"
     CDS             1856..3007
                     /locus_tag="PLN77_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HRM43708.1"
                     /translation="MSTGNQEYIRDMNRALVLEAIANHPPLSRADLAKMLHLTKATVS
                     TIVQELLDRQLVIELGSAEKTSMGRKPILLTFNNRCGSIIAVDLGVKKITILTGDLKG
                     KSCTVKEYPIDPSLSLEEYLISLLHKTKSDLPKTPCGLVGISIGIYGVVCDDLVLFTP
                     YYDLKHSNLKNYLEEEFQVPVIVENEANLSVIGEAACAEHFKNMIFLNIHEGVGMGIL
                     IDGELYTGQNGYAGEFGHTILFPDGRPCPCGNHGCLEQYISEAAVLNDFAAAEGLENV
                     SVERFIQYYQEGNPNARKVMQDFTHYIGISLNTVLHTFNPDVIVINSSFTNYIPGLID
                     EITSGIQNRLRWYLHVLPAHLQDVSVLMGGISLCSRNFLGISTFKLLDL"
     gene            complement(3434..4306)
                     /gene="rsmA"
                     /locus_tag="PLN77_05200"
     CDS             complement(3434..4306)
                     /gene="rsmA"
                     /locus_tag="PLN77_05200"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRM43709.1"
                     /translation="MEKVKPWLGEPQNTIAVLQKYGFVFQKKYGQNFLIDPHVLDKIV
                     AAAGIGPDDFVVEIGPGIGTLTQYLAYAARSVCAVEIDKNLIPILEDTLSDYDNVEVI
                     NNDVLKVDLAALAKEKNNGRPIKVVANLPYYITTPIIMGLFENHVPVDSITVMVQKEV
                     ADRMQTGPGSKDYGALSLAVQYYAEPYIVANVPPNCFMPRPKVGSAVIRLTRHEVMPV
                     ETKDEKLMFSIIRASFNQRRKTLANGLKNSELLHFSKEEIEAAITACGWPLTIRGEAL
                     NLQEFARLTDELCK"
     gene            complement(4322..7216)
                     /locus_tag="PLN77_05205"
     CDS             complement(4322..7216)
                     /locus_tag="PLN77_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43710.1"
                     /translation="MDKAEYQSRLEELNSLVKKEDYEGALAVVEAVDWRRVKSLRTLG
                     MVADVYEANKRYPEAKKILLMAYDRSSIGKGILYRLVEVSVKMKDFDEAIDFYNEFEA
                     VARHDNSRYLLKYKILRGQKAPLEEQISLLEEYKEREFTERWAYELANLYSKAGETQK
                     CIDACDELILWFSEGKYVTKAMDLKMKYEPLSPSQQIKYEHRFDYKKNEEPSEDPEAL
                     EVRLAGESGAGEMAATKEPAETPEVKPVNGGVMSQYVAGEEEKEAGSPYEAYEKTQQA
                     EADAEQVQEYEALQQTEEPIEEIPEEQAYADSTPTYLGNTIDLHAELAKSIRDVFSFR
                     GKKQEETAVEEETSEEPVQYEVKDLEPEQLDVNVEVSPDANTTIKTRPQPKPKEKAEP
                     VMPKAPKPEKKYEEPDLEALFQETAANLSEEVAEEEAEEPVEPAAEAETAEQPMDPES
                     EAEAREAAEEPQAEAEPEEDAEPADEAETEEITEPADEAETEEATEPMDEAEIEEIIE
                     PAAEAEIEEAEVTEPAVEAETEAAEKAQAEAEIEETVEPAETPSMDATVVIADLKPAE
                     ETRPTIDFSELQAALNAEVQEAEKASEIELPDEPETAEAEPADEPESETEPETPAETE
                     PADPFEPAGTQPIEITPEEIEIEVPTEPKRERVSDATINMSIEKTTDATVQLIRDNIP
                     EGTVAKSIEEVLREETPEEARLRILNDTQPDKLSDEQKALFTYFAKVPGMDIQILDAM
                     HGVYHYAGDRTSRHGNIAIMGGYGTGKTKLSEDLVRAICADLKLPATKMARLDASVLN
                     KKDCAQIVSKLAGGFLLIERAGLMYPETIEKLSQAMDFRTDSLVLIIEDEKVSMRGLL
                     RNYPDFAKKFETVISIPVFTNDELVTFARTYARENGYKMDEMGVLALYTQIGNNQKEG
                     EPMTISQVKDMVDRAIAHAEKGTRKFGRRMSRKHTDADDRVILYEKDFEF"
     gene            complement(7424..9886)
                     /locus_tag="PLN77_05210"
     CDS             complement(7424..9886)
                     /locus_tag="PLN77_05210"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRM43711.1"
                     /translation="MLNAEFKKETFKNSVKENVKMLYRKNLEDASQQEIYQAVCYAVK
                     DVIIDNWLKTQKAMEEQDPKILYYMSMEFLMGRALGNNLINLTAYKQVQEALEEMGLN
                     LNLLEDQERDPALGNGGLGRLAACFLDSLATLGYPAYGCGIRYKYGMFKQQIRDGYQV
                     EVPDNWLKNGYPFELKRPEYAKEVKFGGYVAQEYDEATGRVNFVQKDYQSVNAIPYDM
                     PIVGYDNDVVNTLTIWDAEAIQDFSLDSFDKGDYHKAVEQENLAKTIVEVLYPNDNHY
                     EGKELRLKQQYFFISASVQRAVAKYKKQHDDITKLYEKVTFQLNDTHPTVAVPELMRI
                     LMDVEGLGWDEAWEITTKTCAYTNHTIMAEALEKWPVDLFSRLLPRIYQIVEEINRRF
                     LIQVREQYPGDEEKVRQMAVIYDGQVHMARLAIVGGYSVNGVAKLHTEILKKQTLKDF
                     YEMTPEKFNNKTNGITQRRFLLHGNPLLADWVTEYAGNGWITDLPKIEKIAELADDTQ
                     AQKEFMEIKYKNKVRLAKYIKEHNGIDVDPNSIFDVQVKRLHEYKRQFMNILHIMYLY
                     DRLKKNPDMPFYPRTFIFGAKASAGYERAKAIIKLINSVGDVVNNDPTIKGKIKVVFI
                     EDYRVSNAELIFAAADVSEQISTASKEASGTGNMKFMLNGALTLGTMDGANVEIVEEV
                     GKENAFIFGLSADQVIEYENNYTYDPMSYFIHDQDIHNVVMDLISGRYSNGDTMMFRD
                     LYDSLLTPKNAPRPDMYFTLADFKSYAAAQERVEDKYRNTSAWARSAILNTAHSGKFT
                     SDRTIQQYVDDIWHLDKITVRK"
     gene            complement(9994..>10761)
                     /locus_tag="PLN77_05215"
     CDS             complement(9994..>10761)
                     /locus_tag="PLN77_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HRM43712.1"
                     /translation="DFPEVHENWIDPKMEEDMADLLEIVVMGRAARNTANIKNRQPIG
                     TMYVKSEFQLSEFYKEIIEDELNVKEVVFKDDIADFISYSFKPQMRTVGPKYGKLLNK
                     IKTVLSELDGNKAMAELKSTGELKLDIDGQEIVLLEEDLLIDMAQMEGYVSESDHTIT
                     VVLDTNLTPELIEEGFVRELVSKIQTMRKEAGFEVMDKIRVYAKDNDKIVDIMKNHGD
                     EIKSEVLAEDIVTGETKGYEKEWNINSEKVTMAVERI"
BASE COUNT         2393 a         2973 c         2184 g         3211 t
ORIGIN      
        1 tgtattattt ccggcattaa atcggtatta tttgccgaca ttttgtgttg caaacatcct
       61 gttcgcactg ctcttcattt aaaattaaaa gcacattttc ttccttggga tttatcaaaa
      121 agacagctgt acacgaaaaa gacataggaa atgcagaaaa aacgcacaaa aagagagaac
      181 agatttctcc attctctctt caataaattt attctactaa aaatttttta tttaccaaaa
      241 tatctagcca gcagaccagc gaatgctttt ccgtgtctag cttcgtctct tgccatctca
      301 tgcacggtgt catggattgc atcgagattc agggctttgg cacgttttgc aagatctgtt
      361 ttgccagcgg ttgctccgtt ctctgcctct acacgcatct gcagattttt cttggtgctg
      421 tcggttacga cttcgccgag aagctctgcg aattttgctg cgtgctctgc ctcttcgtaa
      481 gctgcttttt catagtacag acctacttcc ggatatccct cgcggaatgc tactctggac
      541 attgccaggt acataccaac ctcggagcac tcaccctcaa aattggctct cagatctttt
      601 ttgatatctt ccggaacatc ggatgctacg ccaactacat gctctgctgc ccaggtcaga
      661 tcctcgccct gctcgataaa tttctctttc ggtgctttac agatcggaca gacttccggt
      721 gcttctgctc cttcataaac atatccacat acgctgcata caaatttttt catagtcttt
      781 ctttcctttc tttttgttgt gtatttaatt tttatcattt ccaattttat ggtttataac
      841 agtttttatt tttaatatta gtaactgtta ctgatattac tttagcataa gattttcttt
      901 ttgtcaatgc ctatttttaa ttttttcttt cttcctgggg atgctatacg ttccccgggt
      961 gatttttgca gttctcgcag atgccgtaaa aattcacaga gtaatcttcg atcgtacctc
     1021 tgaaattttc cgctgctacg ttcttgattt cgctgatgtc acgcattgta aggtctgtca
     1081 cgcagtgaca ttttctgcaa atgaaatggt gatgcgtgga agtatctcca tcgaaccggt
     1141 ccgggccatc tcctgttgta agacgcttga tctctcctaa ttcagaaaga agagacaggt
     1201 tccggtatac tgtgccaaga ctgatattgg gaaattcttt ccgtacatcg gtatagagca
     1261 tatctgcggt cgggtgatcc tttcgcccct gaaggcagac tttgatgctt tctctttgtc
     1321 tgctatactt gagtgttgac atagtttttt tcctttcgta tctttcaata acagtaattg
     1381 ttattatatt tatactataa ggctgcttct cgcaaaagtc aagaaaaaga tcactaccct
     1441 gtctacacag atttccgtct gtcccaagat caaaaatttc ccaaaaacga ggagtgccca
     1501 ggaacctctc ggcgcctggg ctattattat gaaaaaatct taattgtgta gtgattgcat
     1561 acacgctgtt cctttggaac tgagataact ataccaatta attatgaaca aattatgaac
     1621 gtgcagtgaa tcttctgtga atctgtacat tttttagaat tattttatac tttctttgtg
     1681 tatttatcac atattttatt tctaattctg tcccttactc tctactctgt ctagaaatcg
     1741 tttttctccg ttaatttttc tattcgtatt gcccatcccc gattttccct gtcatttctc
     1801 tctcatttat gttaagattg atgtacagcc aatgcggcag aatggaggat tttttatgag
     1861 taccggaaat caggaatata tacgagacat gaaccgcgcc ctggttctcg aggcaatcgc
     1921 caaccatccg ccgctttccc gcgcagatct ggcgaaaatg ctgcatctga caaaagcaac
     1981 cgtatccacc atcgtccagg aactgctcga ccgccagctc gtcatagaac tcggcagcgc
     2041 cgaaaaaact tccatgggtc gaaaaccgat tctgcttact tttaataatc gctgcggttc
     2101 catcatcgcc gtcgatctcg gcgtaaaaaa gatcaccatt ctcaccggag atctgaaggg
     2161 aaaatcatgt actgtaaaag aatacccgat tgatccatcc ctctccctgg aggaatacct
     2221 gatttccctc ctccacaaaa ccaaatccga cctccccaag accccgtgcg gcctcgtcgg
     2281 aatctccatc ggaatctacg gcgtcgtctg cgacgacctc gttttattca caccctacta
     2341 cgatctgaaa cactccaacc tgaaaaacta cctcgaagaa gaatttcagg tccctgtcat
     2401 cgtagaaaat gaagccaacc tctccgtcat cggcgaagcc gcctgcgcag agcactttaa
     2461 aaatatgatc ttcctgaaca tccacgaagg cgtcggcatg ggaatcctca ttgacggcga
     2521 actctacacc ggccagaacg gctacgccgg cgaattcggc cacaccatcc tcttccccga
     2581 tggccggccc tgcccctgcg gcaaccacgg ctgcctcgaa cagtacatct ccgaagccgc
     2641 cgtcctgaac gattttgccg ccgccgaagg cttagaaaac gtctccgtcg aacgcttcat
     2701 ccagtattac caggaaggca accccaacgc ccgcaaagta atgcaggatt tcacccacta
     2761 catcggaatc agcctgaaca cggttctcca caccttcaac ccggacgtca tcgtcatcaa
     2821 cagctccttc accaactaca tcccaggtct gatcgacgaa atcacctccg gaatccagaa
     2881 ccgcctccgc tggtacctcc acgtcctccc ggctcacctg caggatgtct ccgtcctcat
     2941 gggcggaatc agcctctgct cccggaactt cctcggaatt tccaccttta agctgctgga
     3001 cctgtaactc agaaataagt ccctgttcag agccccatct cgattccgct gcgctgcatc
     3061 ttgatgtggc ttctcgccat atgaaatttc agaaaaaagt gctcattcat gcaagcatga
     3121 ttcgcaattt gttctgaaat ttcgcatggc tctgaatagt cacaatttgt tcatgatttt
     3181 tttacaacat gaacacgctt tcaacataat ttacccgtat actaaaagca taaataatga
     3241 atgccaacat tatttacaca attttctttt tcatattgcc gattagaaaa tcggctctcc
     3301 ttcctttaaa aataaaaatg aaagaaagca ctgtttcgcc aacggtgctt tttttcatgc
     3361 ccttttcccc cgcttttcca ccgctgccat gcgtatcacg caagggcgga cgggcgacgg
     3421 cgaggcgtcc cctctattta cacaattcat ccgtcaaccg cgcaaactcc tgtaaattca
     3481 atgcctcccc acgaatcgta agcggccagc cgcaggctgt gattgcagcc tcaatctcct
     3541 ctttcgaaaa atgcagcagc tccgaatttt tcagtccgtt cgccagcgtc ttccgtctct
     3601 gattaaacga cgcccgaata atggagaaca tcaacttctc atccttcgtc tccaccggca
     3661 tcacctcatg acgcgtcagc cggatcaccg cacttccaac cttcggccgc ggcataaaac
     3721 agttcggcgg cacattcgcc acaatatacg gctccgcata atactgcacc gccagcgaca
     3781 gcgccccgta atccttcgat cccggccccg tctgcatccg gtccgcaacc tccttctgaa
     3841 ccataaccgt aatcgaatcc accggcacat gattctcaaa taatcccata ataatcggcg
     3901 tcgtaatata atacggaaga ttcgccacaa ccttgatcgg ccgtccgttg ttcttctcct
     3961 tcgccagcgc cgccagatcc accttcaaaa catcattgtt gataacctca acattatcat
     4021 aatccgacag cgtatcctcc agaatcggga tcagattctt gtcaatttcc accgcacaca
     4081 cactccgcgc agcatacgca agatactgcg tcagcgtacc gataccaggt ccgatttcca
     4141 caacaaaatc gtccggtccg atacctgcag cggctacaat cttatccagc acatgtggat
     4201 cgatcagaaa attctgacca tactttttct gaaatacaaa gccgtacttc tgcagtacgg
     4261 ctattgtatt ctgcggctcc ccgagccacg gttttacttt ctccataaat tctccatgta
     4321 tttagaactc aaagtctttc tcgtaaagaa tcacgcggtc gtccgcatcg gtatgctttc
     4381 tggacatgcg tcttccgaat ttacgggttc ccttttctgc atgggcgatg gcacgatcca
     4441 ccatatcttt gacctgtgaa atcgtcatcg gctcgccctc cttctgattg ttgccaatct
     4501 gtgtatacag agcaagcaca cccatctcgt ccatcttgta tccattctca cgcgcatacg
     4561 tgcgggcaaa cgtaaccagc tcgtcgtttg tgaaaacagg aatcgaaatg accgtctcaa
     4621 acttcttcgc aaaatcaggg taattccgca gaagacctct catgctgacc ttctcatcct
     4681 cgatgatcag aaccagactg tcggtacgga aatccattgc ctgcgacagc ttctcgatcg
     4741 tctccgggta catcagaccg gcacgctcga tcaggaggaa acctcccgcc agctttgaaa
     4801 caatctgcgc acagtccttt ttgttcagaa cagatgcatc cagacgggcc atcttcgttg
     4861 ccggaagttt taagtcagca cagattgcac ggacgagatc ctccgacagc ttcgtttttc
     4921 cagtaccgta tccgcccata atggcaatgt ttccatgacg ggaagtacgg tcgcctgcgt
     4981 aatgataaac accatgcatt gcatccagaa tctggatatc catgcctgga actttcgcaa
     5041 agtaagtaaa cagcgctttc tgctcatcac tcagcttatc cggctgggtg tcgttcagga
     5101 tgcggagtct tgcctcctcc ggcgtctcct cgcggagcac ctcttcaatc gatttcgcaa
     5161 cagttccttc cggaatgttg tcgcggatca gctgaacggt ggcatccgtt gttttttcga
     5221 tgctcatgtt gatcgtcgca tcggatacgc gctcacgctt tggctcggtc ggaacttcga
     5281 tttcgatttc ctccggggta atctcgatcg gctgggttcc tgccggctca aacggatcgg
     5341 ctggctcagt ctcggccggg gtttccggct ctgtttcgct ttccggctcg tcagcaggtt
     5401 ctgcttccgc agtttccggc tcatccggca gttcgatttc cgatgctttt tcagcttcct
     5461 gcacttctgc gttcagcgct gcctgaagct cactaaaatc gatggtcggc cgtgtctcct
     5521 ctgccggttt cagatccgcg atgacaaccg ttgcatccat ggacggtgtc tccgccggtt
     5581 ctacggtttc ctcaatttcg gcttccgcct gagccttctc ggctgcttct gtttcagctt
     5641 ccactgctgg ttctgtaact tctgcttctt cgatttcagc ctcggctgcc ggttctataa
     5701 tctcttcgat ctcagcttca tccatcggct ctgtagcttc ttcggtttca gcctcatccg
     5761 ccggttctgt gatctcttcg gtttccgctt catctgccgg ttctgcatct tcttccggct
     5821 cagcttccgc ctgcggctcc tctgccgctt cccgtgcctc tgcctcggac tccggatcca
     5881 tcggctgctc tgcggtttcg gcttctgctg ccggttccac tggctcttct gcttcttcct
     5941 ccgcaacttc ctccgacagg tttgctgcgg tctcctggaa cagtgcttcg aggtccggct
     6001 cttcgtattt cttctccggc ttcggtgctt tcggcattac cggctcggct ttttccttcg
     6061 gttttggctg cggtcttgtc ttgattgtcg tgttggcatc cggggatacc tctacgttta
     6121 catcaagctg ctccggctcc agatctttta cttcgtactg taccggctct tcgcttgtct
     6181 cttcctcgac tgccgtctct tcctgtttct ttccgcggaa ggagaagaca tcgcggatgc
     6241 tctttgcaag ctctgcgtga agatcgatcg tatttccaag gtacgtcggc gtgctgtctg
     6301 catacgcctg ctcttccgga atctcctcga tcggctcctc tgtctgctgc agtgcttcgt
     6361 actcctgcac ctgctctgca tctgcctcgg cctgctgtgt tttctcatat gcttcgtacg
     6421 gcgaaccggc ttctttttct tcttcaccgg ccacatactg gctcatcaca ccgccgttta
     6481 ccggttttac ttccggcgtc tctgccggct ctttcgtcgc agccatttca ccggctccgc
     6541 tctctccggc taaccgcact tcaagtgctt ccggatcctc ggacggctct tcattctttt
     6601 tgtagtcaaa acgatgctcg tatttgatct gctgggacgg tgacagcggc tcgtacttca
     6661 ttttcagatc cattgccttt gtcacatatt tgccctcgct gaaccacagg atcagctcat
     6721 cacaggcatc gatgcatttc tgcgtctctc cggctttgct gtaaaggttt gccagttcat
     6781 atgcccatct ctcggtaaat tcccgctctt tgtattcctc cagaagggaa atctgctcct
     6841 caagcggcgc tttctgtcct cttaaaatct tgtatttcag cagataccgg gaattatcgt
     6901 gacgtgcgac tgcttcaaac tcgttgtaaa agtcaatcgc ctcatcaaag tctttcattt
     6961 ttacggagac ctctaccagg cggtacagaa tgccctttcc gatggaggaa cggtcatacg
     7021 ccatcagcag aatcttcttc gcctccggat atcgtttgtt cgcctcatag acatccgcta
     7081 ccatgccgag cgtgcgcaga ctcttgacac gccgccagtc aacggcttct acaactgcaa
     7141 gtgccccctc atagtcctct ttttttacca gactgttcag ctcttccagc cggctttgat
     7201 attcagcttt atccaatctt ttcacctcaa tacatttcag attgttttag ttcatagaca
     7261 cgaacagtgt accacactcc ccgtagaatg tcaaaagaat gcctgttttg caggcatttt
     7321 tcgggatgca aatccttcca gcggtcatca aaaaaagaac acccgcccct ccggtttttc
     7381 ccggaaaagc aggtgtcccc taactactta tatttgattc cgattacttt ctgacagtaa
     7441 tcttgtccag atgccagata tcatctacat actgctggat ggtacggtcg gatgtgaatt
     7501 tgccgctgtg tgcggtattc aggatcgcgc ttcttgccca tgcagaagta tttctgtatt
     7561 tatcctcaac acgctcctgt gctgccgcat aggacttgaa gtctgccagg gtaaagtaca
     7621 tatccgggcg cggagcattc ttcggtgtga gcagggagtc atacagatca cggaacatca
     7681 tggtgtcgcc gtttgagtat cttccgctga tcagatccat cactacgttg tggatgtcct
     7741 gatcgtggat gaagtagctc atcggatcgt aggtgtagtt gttctcatat tcgataacct
     7801 gatctgcgga aagtccgaag ataaaggcgt tctcctttcc aacttcctca acgatctcta
     7861 cgtttgcacc gtccatggtt ccaagagtca gggcaccgtt cagcatgaac ttcatgttac
     7921 cggtaccgga agcctcttta cttgcggtgg aaatctgctc ggaaacatca gcagcggcaa
     7981 agatcagctc tgcgttgctg acacgataat cctcgataaa tacaactttg atctttcctt
     8041 tgatggtcgg atcgttgttt acgacatccc caacggaatt gatcagtttg atgattgctt
     8101 ttgcacgctc atagcctgcg gaagcttttg ctccaaagat gaaggttctt gggtagaacg
     8161 gcatatccgg gttctttttc agacggtcat acaggtacat aatatgaaga atgttcataa
     8221 actggcgttt gtactcatgc agacgcttta cctgaacgtc aaaaatagag ttcggatcta
     8281 catcaatgcc gttatgctct ttgatgtatt ttgccaggcg aactttattt ttgtatttaa
     8341 tctccataaa ttccttctgt gcctgtgtat catctgcaag ctcagcgatc ttttcgatct
     8401 tcggaaggtc agtaatccag ccatttccgg catactcagt tacccagtca gccagaagcg
     8461 gatttccgtg aagcaggaaa cgacgctgtg taataccgtt cgtcttattg ttgaacttct
     8521 ccggagtcat ttcgtagaag tcttttaagg tctgtttttt caggatctcg gtatgaagct
     8581 ttgcaacacc gttgacagaa tatccgccta cgatagccag acgagccata tgaacctgtc
     8641 cgtcatagat cactgccatc tggcggactt tttcctcatc acccgggtac tgctctctta
     8701 cctggatcag gaagcggcgg ttgatctctt ctacgatctg atagatacgc ggaagaagtc
     8761 tggagaacag gtcaaccggc catttttcaa gtgcctctgc cataatcgta tggttcgtgt
     8821 atgcacaggt ctttgtcgta atctcccatg cctcatccca gccaaggcct tccacatcca
     8881 tcagaattcg catcagctcc ggaacagcta cggtcgggtg ggtatcattc agctggaagg
     8941 taaccttctc atacagcttc gtaatatcat cgtgctgttt tttatatttt gcaactgctc
     9001 tctgtacgct tgcagaaata aagaaatact gctgtttcag acgcagctct tttccttcgt
     9061 aatggttatc gttcggatac agaacctcta cgatggtctt tgcaaggttt tcctgctcaa
     9121 cagctttgtg gtaatcaccc ttgtcgaagg aatccaatga gaagtcctga atagcctctg
     9181 catcccagat agtcagagta tttacaacgt cgttgtcata tcctacgatc ggcatgtcgt
     9241 aaggaattgc attgacagac tggtaatcct tctgcacaaa gtttacgcgt ccggtagcct
     9301 catcgtactc ctgcgccaca tatccgccga attttacctc ttttgcatac tccggacgtt
     9361 tcagctcgaa tggatagccg tttttcagcc agttatccgg tacttctacc tgataaccgt
     9421 cgcggatctg ctgtttgaac ataccgtact tgtaacggat accgcagccg tacgccggat
     9481 atccaagagt agccagagaa tccagaaagc atgctgccag acggccaagg ccgccgtttc
     9541 caagagccgg atctctctcc tgatcctcca gaaggttcag gttcagtccc atttcctcga
     9601 gagcttcctg tacctgttta tatgcagtca ggttgatcag gttatttcca agtgcacggc
     9661 ccatcaggaa ttccatggac atatagtaca gaatcttcgg atcctgttct tccattgcct
     9721 tctgggtttt cagccagtta tcaataataa catcttttac tgcatagcag actgcctgat
     9781 aaatttcctg ctgggaagca tcttccaggt tctttctgta cagcattttt acattttctt
     9841 tcacactgtt cttaaaagtt tcttttttga actcggcgtt taacatctgc gtcctcctta
     9901 atagcagtct gcgagcggag agcttgtttt tctccccgct cgcaggccaa tttcatatta
     9961 atgatcgtcg tgaaaacaca tccaaaaata atcttagata cgttctactg ccatggtaac
    10021 cttttcggag ttgatattcc actccttctc atagcccttt gtctctccgg tcacgatatc
    10081 ttctgccaga acttcagatt taatttcatc accatggttt ttcatgatgt ctacaatctt
    10141 atcgttgtct tttgcgtata cacggatctt gtccataacc tcaaagcctg cctctttacg
    10201 catagtctgg attttgctta ccagctcacg tacgaagcct tcttcgatca gttccggagt
    10261 cagattggta tccagaacaa cagtgattgt atggtcggat tcggaaacat agccttccat
    10321 ctgtgccatg tcgatcagca aatcctcctc taataataca atttcctgtc catcgatgtc
    10381 aagtttaagt tcgccggtgc tcttcagctc tgccattgcc ttgtttccat cgagctcgga
    10441 cagtacggtt ttgatcttgt tcaggagttt tccgtatttt ggtcctacgg tacgcatctg
    10501 cggtttgaag ctgtagctga tgaaatctgc gatgtcatct ttgaatacaa cttctttgac
    10561 attcagctcg tcctcgatga tctctttgta gaattcggaa agctggaact cgctcttcac
    10621 atacatcgtt ccgatcggct gacggttctt gatattggcg gtgtttcttg cggcacgtcc
    10681 cataacgacg atctccagca ggtcggccat atcctcttcc atcttcggat cgatccagtt
    10741 ctcatgtacc tccgggaagt c
//
LOCUS       DAOMUF010000064        10672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1497_length_10672_cov_3.266459, whole genome shotgun sequence.
ACCESSION   DAOMUF010000064 DAOMUF010000000
VERSION     DAOMUF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1497_length_10672_cov_3.266459"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1785)
                     /locus_tag="PLN77_05220"
     CDS             complement(127..1785)
                     /locus_tag="PLN77_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM43713.1"
                     /translation="MRKKVFQHAGALIIAAIILTFFTMSWAMYNNTAEQLRVSVRNEC
                     EYYRYFLDKMGDDSLDKPAGKITASRVTLIDTDGTVLFDSVEDENMMGNHSGRPEVIE
                     AEKTGESNISRYSETLRSKTYYTALRLDNGKIIRVSLTTDSVFGVMLRNIWLVVVLIA
                     AVLVVELMMVSHMTKALVKPINEIDLNHPLDNKAYEELSPLLGRIHQQQKQIQQQMEE
                     LRHNQEEYLAITEYMKDGLIVTNQSVVLSINRSAQNLFDVTAEECINHNIVTVSRNEQ
                     LKEALDHALEGSSEERMLEINGRVYQLLANPVRVDRVVSGAVILVLDVTEKQKAEVMR
                     REFSANVSHELKTPLMSISGYAEIIENNMVKPEDIPKFAGRIHSEASRLSSLVEDIIK
                     LSRLDENDQSIPMEEVDLMQICRDVEGHLSIRAKEQQVKMTLRGESCRIKGARQVLYE
                     MIYNLCDNAIKYNRKDGEVEVTVSRGRNGRAVVSVADTGIGIAKEDQERIFERFYRVD
                     KSHSRETGGTGLGLSIVKHGAILHDAKIKIESTLGTGTKIILEF"
     gene            complement(1795..2466)
                     /locus_tag="PLN77_05225"
     CDS             complement(1795..2466)
                     /locus_tag="PLN77_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM43714.1"
                     /translation="MIYCVEDERNIRELIVYTLESSGFTARGLESGKELNAAIAEQLP
                     DLILLDIMLPGEDGLEILSRLKASEVTKEIPVIMVTAKGAEYDKVMGLDCGADDYITK
                     PFGMMEFIARVRAVLRRAGKEEKENDVLETGDLQIQVKQHQVLAKGEKVTLTLKEFEL
                     LKFLMENKGIVLTRDRLLGHVWGYDFDGETRTVDVHVRTLRQKLGECGELIETVRGVG
                     YRIGG"
     gene            complement(2470..3114)
                     /gene="phoU"
                     /locus_tag="PLN77_05230"
     CDS             complement(2470..3114)
                     /gene="phoU"
                     /locus_tag="PLN77_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRM43715.1"
                     /translation="MRERFEQQMEILHTELIELGALCEQAIGRTYEVLLEGNQRAAEE
                     IIKKDQVVDAKEREIEDLCFRIILQQQPVARDLRQVSAALKMITDMERIGDQAADIAD
                     IVKVTSLGVPDASIRLKDMAKATASMVTKCIDAYVKQDLKLAREVIDFDDIVDDLFDE
                     VKDEILQGMRKGITTDVQSLDYLMIAKYYERIGDHATNIAEWVVYSINGHHKGE"
     gene            complement(3116..3868)
                     /gene="pstB"
                     /locus_tag="PLN77_05235"
     CDS             complement(3116..3868)
                     /gene="pstB"
                     /locus_tag="PLN77_05235"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRM43716.1"
                     /translation="MSKIDVSNMDLYYGDFHALKNINLSIDANEVTAFIGPSGCGKST
                     LLKSINRMNDLVPGCRITGEMLLDGENIYGGKMDVNGLRKRVGMVFQKPNPFPMSIYD
                     NIAYGPRTHGVRSKAKLDDIVEQSLRQAAIWDEVKDRLKKSALGMSGGQQQRLCIARA
                     LAVQPEVLLMDEPTSALDPISTSKIEDLAVELKKDYTIVMVTHNMQQAARISDKTAFF
                     LLGEVVEYGETEQIFSMPKDKRTEDYITGRFG"
     gene            complement(3889..4791)
                     /gene="pstA"
                     /locus_tag="PLN77_05240"
     CDS             complement(3889..4791)
                     /gene="pstA"
                     /locus_tag="PLN77_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRM43717.1"
                     /translation="MVIKGNGAVNTMEAVKINTKEKERTKVTYHKSVFSSVLTGITWG
                     AAILALAVLVFLVGFILIRGVKNLTLEQFAWTYNTENVTMLPAIINTVTMTVLSLLLA
                     APLGIFSAIYLVEYANKRGKLVGLIRITAETLSGIPSIVYGLFGMLFFTKTLKMGYSM
                     MSGACTLAIMILPLIMRTAEEALKSVPDSYREASFGLGAGKLRTIFKIVLPSAVPGIL
                     SGVILATGRVVGETAALLFTAGTIAKVAGLMDSGRTLAVHMYALSSEGLYMNQMYATG
                     VILMLIVLLINGCSGMIAKKIAKK"
     gene            complement(4781..5614)
                     /gene="pstC"
                     /locus_tag="PLN77_05245"
     CDS             complement(4781..5614)
                     /gene="pstC"
                     /locus_tag="PLN77_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRM43718.1"
                     /translation="MKLVFLIAACCSVLAVALICIFLFMNGVPAIFKIGPLKFLTGTV
                     WKPGNNIFGILPMIVGSICVTGLAILMGVPVAILTSVFLSRYCPKKFYGICKSGINLM
                     AGIPSIVYGFFGLVVIVPLMAQLTGKNGNTMLTASILLAVMILPTVVGVTESAITSVP
                     ESYYEASLGLGATHSQSVFFAVVPAAKSGILAGIVLGIGRAIGETMAVIMIAGNQPRM
                     PKGITEGLRTMTANIVLEMGYASGLHREALIATGVVLFVFILLINLSVSMLNRRVHYG
                     D"
     gene            complement(5827..6783)
                     /locus_tag="PLN77_05250"
     CDS             complement(5827..6783)
                     /locus_tag="PLN77_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRM43719.1"
                     /translation="MKRKVIAITLTAMMAAGMLAGCGSSDSSSSLAADTTKEASATEA
                     DGSTDGDSNAAGGDFSGQISVISREDGSGTRGAFIELFGVEEKNDAGEKVDNTTVDAQ
                     ITNNTSVMMSTVAGNQYAIGYISLGSLNDEVKALKIDGAEASAENVENGSYKVSRPFN
                     IVTKDGLSADAQDFMDYILSTDGQQVVSDDGYIAIKDTKAYEGNCSGEKVVVAGSSSV
                     TPLMEKLKEAYTKVNSNANIEVQQSDSTTGITSASDGLCDIGMASRDLKDEEKSSGLT
                     ATVIATDGIAVIVNKENPTDGLTSDQVKSIYVGDTTDWADVK"
     gene            complement(7173..8309)
                     /locus_tag="PLN77_05255"
     CDS             complement(7173..8309)
                     /locus_tag="PLN77_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM43720.1"
                     /translation="MSVDVTKMPKLGFGMMRLPEKDGELDLEQICKMVDAYLASGMNY
                     FDTAYMYCGGKSESIVKKALVERHPRESFTLTTKLPQWMMDEGIEGRDRIFNDQLART
                     GAGYFDYYLLHSVEDGANYEGYVKYDCFNWAKKKKEEGLIKHFGFSFHGTPELLDKIL
                     SEHPEVEIVQIQMNYADWDNPLIQSGRLYEVLRKYNMPFLVMEPVKGGSLASAGKEIE
                     AEMKRVHPDASIASWALRFAASLPGVATVLSGMSNEEQMEDNIKTFRNFVPLNEEEKA
                     VIAKAQEALKKSPAVPCTACRYCCDGCPMGIAIPDVFKALNTIRLYGEEDRAKNYYKK
                     LLETSGKAEDCVQCGQCESVCPQHLPIIDLLKEASEKLDVPMTE"
     gene            8667..9755
                     /locus_tag="PLN77_05260"
     CDS             8667..9755
                     /locus_tag="PLN77_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM43721.1"
                     /translation="MSEKNTRKQIRTLYLMTTVGYFQIAAASWVALLALRGFSLLQIG
                     MLESIFHIVSLCFELPSGVVADVFGRRKTLIASQMASLISGTLMIFSTGFATTALPLG
                     CSALSYNLASGTREALAYDSLKQNGDEGFYARFSSTEMMLYRITNSTATLCAGLALWL
                     GYRRAYAIDLFFGLIALGVSFRLVEIKTDETPVHYPVGQEIVSVVQESWQFLVREKKA
                     RNIMLVNALIGAVSTLVLFFLQAKLPLTGMNSALLGPALFVMGLGAALGSRMTAYFHK
                     YRYRAILLFGTIGVLFAFLMSFTGLPALMVFGGFIGSFSDDFLEVRTDILLNDMIPSK
                     QRATLMSVNSFVFSMIMIVLSTVFGWIM"
     gene            complement(9829..>10672)
                     /locus_tag="PLN77_05265"
     CDS             complement(9829..>10672)
                     /locus_tag="PLN77_05265"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRM43722.1"
                     /translation="MAEHGVVCVIHDHRGHGKSVKSEQDLGFMYGAGGVGLVEDLFLV
                     TKWAKKEYPDLPFVLMGHSMGSLVVRAYAKEHDQELDALIVCGSPSKNYLRPLGAAVG
                     HAEAAVLGDEHRSNLLEAMSFGSFAARFADEKSRFAWCCSDPEVVREYEENPLCGFTF
                     SDDAFFALNDLLKETYGFHGWHCTNRKLPVLFLSGGDDPCYVNVRRFKKSIDHMRLIG
                     YRNVRGKLYPGMRHEILNEKEKYRVYGDVWKWLKKEKVVENVEGGSTPPQTPDGALAG
                     SGGV"
BASE COUNT         2498 a         2890 c         2238 g         3046 t
ORIGIN      
        1 catacataaa aagacagcat gcgggggggg cggaaccgct ttgggcactt aaaaatcatg
       61 aatttttcaa gcctcccata taggttctgt ccccgcatgc tgtcttttgc ggtatctgtt
      121 tttcacttaa aactccagaa taatctttgt tccggttcca agcgtactct caattttaat
      181 cttcgcatca tgaagaatcg ctccatgctt cacaatggaa agtccaagtc cggtacctcc
      241 cgtttcgcgg gaatgactct tatccacacg atagaaccgt tcaaagatcc gctcctgatc
      301 ttcctttgca attccaattc cggtatctgc cacagatacc actgcccgtc cgtttcttcc
      361 gcgtgaaacc gtcacttcaa cttcaccgtc cttgcggttg tacttgatcg cattgtcgca
      421 gagattgtag atcatctcat agagaacctg ccgtgcacct ttgatccggc agctctctcc
      481 tctcagtgtc atcttcacct gctgctcctt tgcccgtatg gaaagatgtc cctcgacatc
      541 acggcagatc tgcatcagat ccacttcctc catcgggatt gactggtcgt tttcgtccag
      601 tctggacagc ttgataatat cctcgacaag agagcttaac cggctcgcct cactgtggat
      661 tctgcccgca aatttgggaa tatcctccgg ttttaccatg ttgttttcga tgatctccgc
      721 atatccggaa atcgacatca gcggtgtttt cagctcatgt gacacattgg ctgaaaactc
      781 acggcgcatc acctcggctt tctgtttttc cgtcacatcc agcaccagga ttaccgcacc
      841 ggatacaaca cggtccacac gcaccggatt tgcaagaagc tgatagactc ttccatttat
      901 ctccagcatc cgttcctcgg aactgccctc cagcgcatga tccagtgcct ccttcagctg
      961 ctcgttgcgg ctgactgtca cgatattgtg gttgatgcac tcctccgctg tcacatcaaa
     1021 cagattctgt gccgaacggt tgatggaaag tacgaccgac tggtttgtca cgatcagacc
     1081 atccttcata tattccgtga ttgcaagata ctcctcctga ttatggcgga gctcctccat
     1141 ctgctgctga atctgcttct gctgctgatg aattcttcca agcagcggac tcaattcctc
     1201 atacgctttg ttgtccagcg gatgattcag atcaatctca ttgatcggct ttacaagcgc
     1261 ctttgtcata tggctgacca tcatcagctc cacgacaagg actgccgcaa tcagtacaac
     1321 caccagccat atattccgca gcatcacacc gaacacactg tctgttgtca gggaaacacg
     1381 gatgattttt ccattgtcca ggcgcaatgc cgtataatat gttttgctcc gcagggtctc
     1441 ggaataacgg gaaatattgc tttccccggt cttttccgcc tcaatcacct ccggccgccc
     1501 ggaatggttt cccatcatgt tctcatcctc gaccgaatca aacagaacgg ttccgtccgt
     1561 gtcaatcagg gtaacccggc ttgccgtaat cttgccggcc ggtttatcca gactgtcatc
     1621 acccatcttg tccagaaaat acctgtaata ctcacattca tttctcaccg aaacacgaag
     1681 ctgctccgcc gtattgttgt acattgccca gctcatggtg aaaaaagtca ggataatcgc
     1741 agcgataatc aacgctcccg catgttgaaa gactttttta cgcatcttac tttctcagcc
     1801 tcctatccgg tatcccacgc cgcggaccgt ctcgatcagc tcgccgcatt cgcccagctt
     1861 ctggcggagt gtacgcacat gaacgtctac cgtacgggtt tctccgtcaa aatcatagcc
     1921 ccagacatgg ccgagcagac ggtctctcgt aaggacaatt cctttatttt ccattaaaaa
     1981 tttcagcagt tcaaactctt ttaaggtcag ggtaaccttc tcccctttgg ccagaacctg
     2041 atgctgcttt acctggatct gcagatcccc ggtctccagc acatcgtttt ccttctcttc
     2101 ttttccggca cggcgcagca cggcacgtac ccttgcgata aattccatca tgccgaacgg
     2161 cttggtaata tagtcatcgg ctccgcagtc aaggcccatg accttatcat actcagcgcc
     2221 ctttgccgtt accataatga ccggaatttc ttttgttact tccgatgcct tgaggcgtga
     2281 aagaatttca agaccatcct ctcccggaag catgatatcc aggagaatca gatccggaag
     2341 ctgttctgca atagctgcat tcagctcttt tccgctttcc aggcctcttg cggtaaaacc
     2401 gctcgattcc agtgtgtata cgatcagttc ccgtatattt ctttcatctt ccacgcagta
     2461 tatcatctgt tattcccctt tatggtgacc gttaatggag tacacaaccc attccgcaat
     2521 gtttgttgca tggtcaccga ttctttcata atattttgca atcatcagat agtccagaga
     2581 ctggacatct gtggtaattc cctttcgcat accctgcaga atttcatcct tgacctcatc
     2641 aaaaagatca tccacgatat catcgaaatc gataacctcc cgtgcaagct tcagatcctg
     2701 cttaacgtat gcatcgatgc attttgttac catagaagct gttgctttcg ccatatcctt
     2761 cagacggatg cttgcatccg gcacaccaag actcgttact tttacgatat ccgcaatatc
     2821 cgctgcctgg tctccgatcc gctccatatc ggtaatcatt tttaatgccg cagatacctg
     2881 acgcagatca cgtgctaccg gctgctgctg taaaatgatg cggaagcaga gatcctcgat
     2941 ctcccgttct ttcgcatcga caacctggtc ttttttgata atctcttctg ccgctctctg
     3001 gtttccctcc agcagaacct catacgtccg tccgattgcc tgctcgcaga gtgcgccaag
     3061 ctcaatcagc tccgtgtgca gaatttccat ctgctgctca aatctttccc gcatatcagc
     3121 caaacctccc tgtaatataa tcctctgtcc gtttatcctt cggcatggag aagatctgtt
     3181 cggtctctcc gtattctact acctctccta acaggaaaaa tgcggttttg tcagaaattc
     3241 tggctgcctg ctgcatattg tgtgttacca tgacaatagt ataatctttt ttcagttcta
     3301 ccgcaagatc ttcgattttg gaagtagaaa tcggatccag tgctgaggtt ggttcgtcca
     3361 tcagaagaac ctccggctgt acggcaaggg cacgcgcaat gcagagacgc tgctgctgtc
     3421 cgccggacat gccaagtgcg cttttcttca aacggtcctt cacctcatcc cagattgctg
     3481 cctgtcgaag agactgctcc acaatatcat ccagcttcgc tttggaacgg acaccatgtg
     3541 ttctcggtcc gtaggcaatg ttgtcgtaaa tgctcatcgg gaacggattt ggtttctgga
     3601 ataccatgcc gacccgtttg cggagtccgt tgacatccat ttttccgccg tagatgtttt
     3661 ctccgtccaa cagcatttca ccggtgatcc ggcagcccgg aacgagatca ttcattctgt
     3721 tgatggattt taacagggtc gatttaccac atccggaagg tccgataaaa gccgtcactt
     3781 cattggcatc aatgctcagg ttgatatttt ttaatgcatg aaagtctccg taataaagat
     3841 ccatattgga tacatctatt ttgctcatgt tttttcctcg tctctctttt atttctttgc
     3901 aattttcttc gcaatcattc cggaacagcc gttgatcaga aggacaatca gcatcaggat
     3961 gacgccggtt gcatacatct gattcatata aaggccttca ctggaaagtg catacatatg
     4021 aacggcaagg gtacggccgg aatccataag tcctgccacc tttgcaatcg ttcctgctgt
     4081 gaaaagaagt gctgccgtct ctcctacgac acggcctgtc gcaagaatga caccggaaag
     4141 aatgccgggt acggcagatg gaagaacaat tttaaaaatc gtacgaagct ttccggctcc
     4201 cagtccaaag cttgcttccc gataggaatc cggaaccgat tttaaggctt cttctgctgt
     4261 tcgcataatc agcggaagaa tcatgatggc aagggtacag gcaccggaca tcatcgaata
     4321 tcccattttc agggttttcg taaaaaacag cataccgaac aaaccgtaaa cgatcgacgg
     4381 aataccggaa agggtctctg ccgtaatccg gatcaggcca accagttttc cgcgtttgtt
     4441 ggcatattca accagataaa tcgccgagaa aatgccaagc ggcgcagcaa gcagcaggga
     4501 aagtactgtc atcgtaacag tgttgatgat cgccggaagc atcgtaacat tctctgtatt
     4561 ataggtccag gcaaactgct ccagtgtcag gtttttcacg ccacgaatca gaatgaatcc
     4621 aaccaggaaa accaaaacgg caagtgcaag aatggctgct ccccaggtaa ttccggtcag
     4681 cacggaggaa aaaacacttt tgtgatacgt aacctttgtt ctttcttttt cttttgtatt
     4741 tatttttaca gcttccattg tatttacagc tccatttcct ttaatcacca taatgtaccc
     4801 tccggttcag catagacacc gataaattga tcagcaggat aaagacaaac agcacaacac
     4861 ccgttgcaat cagcgcttcc cggtgaagtc cggatgcata tcccatctcc agtacaatat
     4921 tggctgtcat ggtacgaagg ccttctgtga ttccttttgg catacgaggc tggtttccgg
     4981 caatcataat cactgccatt gtctctccga tggcacgtcc gatgccaagc acgatacctg
     5041 ccaggattcc ggattttgcc gccggaacaa ctgcaaagaa aacgctctgg gaatgcgtgg
     5101 caccaaggcc aaggcttgct tcatagtaag attccggaac acttgtaatg gcggattctg
     5161 ttacaccgac cacggtcgga agaatcataa cggccagaag aatagatgcc gtcagcattg
     5221 tgtttccgtt tttgcctgtc agctgtgcca tcaacgggac gatcacaacc agtccgaaaa
     5281 atccatatac gatagacgga ataccggcca tcagattaat acctgatttg cagatgccgt
     5341 aaaacttttt cggacagtaa cgggaaagaa acaccgaagt aagaatggca accggcacac
     5401 ccatcaggat tgcaaggccg gtcacgcaga tactgccgac aatcatcggc aggataccga
     5461 aaatgttgtt tcccggtttc cacacggtac cggtcaaaaa tttcagcggt ccgattttaa
     5521 aaatggcagg aacgccattc atgaacagga aaatgcagat cagtgcaaca gccagtacgg
     5581 agcagcacgc cgcaatcagg aatacaagtt tcatgatcgt ttctcttagt ttcataagat
     5641 aaatcctttc atttcattgt ttcgcggagc gtgtttgaaa aagactttcg ttttttttcc
     5701 ttacgaactg ctttttcaag cacgttccga aagacagctt ctttttctga acattttcgg
     5761 atacggaagt aagagcgatt ttccattgtt cgcccttact tctttttcca ttgctttata
     5821 cattctttat tttacatctg cccagtcggt tgtgtcgcca acatagatgc tctttacctg
     5881 atcgctggtc agcccatcgg tcgggttctc tttgtttacg atgacagcaa taccgtcggt
     5941 tgcgattacg gttgcggtaa gccctgagga tttctcctca tcctttaagt cacgggatgc
     6001 cataccaatg tcacagaggc catcgcttgc ggaagtgata cctgttgtgg aatcgctctg
     6061 ctgtacttcg atgttagcgt ttgaatttac ttttgtataa gcctctttta atttttccat
     6121 cagtggtgtt acagaggaag atcctgctac cacaaccttt tcaccgctgc agtttccttc
     6181 gtaggctttg gtatctttga ttgcaatata tccgtcatct gaaacaacct gctgtccgtc
     6241 ggtgcttaag atgtagtcca tgaaatcctg cgcatctgcg gaaagaccgt cttttgttac
     6301 aatgttgaac ggacggctta ctttatagga accgttttct acgttctctg cgcttgcctc
     6361 tgctccgtcg attttcagtg ctttgacttc atcatttaag gatccaaggg agatataacc
     6421 gattgcgtac tggttacctg ccaccgtgct catcataacg gaagtattgt tggtgatctg
     6481 tgcatcaacg gtggtgttgt ctactttctc acctgcatcg tttttctctt ctacaccgaa
     6541 cagctcgatg aatgcacctc tggtaccgga accatcctca cgggagatca cgctgatctg
     6601 tccggagaaa tctccgcctg ctgcgttgct gtcaccgtct gtgctgccat ctgcctctgt
     6661 tgcagaagcc tcttttgtag tgtctgctgc caaagaactg ctgctgtcgc tgcttccaca
     6721 tcctgcaagc attcctgcag ccatcattgc ggttaaggtg attgcaatta cttttctctt
     6781 cataatattc ttttctcctc ttttcacacg ggatttttgt gatttctatt ttgtaaaatc
     6841 gcaatcccga cgttttttac atttcgtttt tacaggtatc atcataacaa cggcgtgtaa
     6901 aatgcaaaat ctcagagatg ttaagtttat gtaaaacatg agtttttcct tttttacatt
     6961 ccattttccg gtcgtaaacc gctccgtcac ctctctcgca agtggcacat tttttccgcg
     7021 cagtgcgatc ctgcccaaag gatctttttc tccgcaggaa atttcaacaa gatttcccat
     7081 agaaaaaaag gctccccgga atactctttt ttcagagtat tccggggagc ctttttctaa
     7141 agcatatgcc aaagcggata ttcgcaatcc gcttattccg tcatcggcac gtcaagtttt
     7201 tcggatgcct cttttaacag atcaatgatc ggcagatgct gcggacatac gctctcgcac
     7261 tggccgcact gcacgcagtc ttctgctttt cccgatgttt ccagcagctt tttataataa
     7321 tttttcgcgc ggtcttcttc tccatacagg cggatggtat tcaatgcctt gaatacatcc
     7381 ggaatcgcga ttcccatcgg acatccgtcg cagcagtaac gacaggcggt acatggaacc
     7441 gccggactct ttttcagcgc ttcctgtgct ttggcgatca ctgctttttc ttcctcgttt
     7501 agcggtacaa aatttcggaa ggttttgata ttatcctcca tctgttcctc gtttgacata
     7561 ccggagagca cggttgcaac acccgggagg gaagccgcaa agcggagtgc ccaggacgca
     7621 atcgatgcat ccggatgaac acgtttcatc tctgcttcaa tctctttgcc tgccgatgca
     7681 agggaaccgc cctttaccgg ctccataaca aggaatggca tattgtattt ccgaagcacc
     7741 tcatacagac gtccggactg gatcagcgga ttgtcccagt cagcatagtt catctggatc
     7801 tggacaattt cgacctccgg atgctcggag agaatcttgt caagcagctc cggcgttccg
     7861 tggaaggaga aaccaaagtg tttgatcagt ccctcttctt ttttcttctt ggcccagtta
     7921 aaacagtcgt atttcacata gccttcatag tttgcgccgt cctcgacact gtgcagcagg
     7981 taataatcaa agtagcctgc tccggtgcgc gcgagctgat cgttgaaaat gcggtcacgc
     8041 ccctcgatgc cctcgtccat catccactgc ggaagtttgg ttgtcagagt aaagctttct
     8101 ctcggatgac gctctaccag cgcttttttc acgatgctct ccgattttcc gccgcagtac
     8161 atataggcgg tgtcaaaata gttcattccg gaggcaaggt acgcatcaac cattttacag
     8221 atctgctcca aatccagttc tccgtccttc tccggaaggc gcatcatgcc aaatcctaat
     8281 ttcggcattt ttgttacatc tacactcatg gtaaaatccc ctttcttttc cacagaaaac
     8341 cttcgttttc tcagtattgt tgaaaaaatt gtagcattaa agtgtacttg aaagtcaata
     8401 gtttttttaa ttttcgtcaa aatttttctt tgactttttc cgtttccggt ggtattctga
     8461 taaaaaaatt atgattacaa aaggagtagc actatgatct ggaaaaacca gtttcttcac
     8521 ctcacaaatg gcgatgtttc agctgtttat ggggatagtg cgctcttttt taagtaaact
     8581 tcactttttg agttcttaaa attctataga aaacgcacat atctcccaat caaaatttta
     8641 ctatattttg acaaggagat ttttttatgt ctgagaaaaa taccagaaaa caaatacgaa
     8701 ctttatatct tatgaccacg gtcggctatt ttcagattgc ggcggcttcg tgggttgcac
     8761 tgctcgcact ccgcggattt tcgctgctgc agatcgggat gctcgaaagt attttccata
     8821 tcgtgagcct ctgctttgaa ctcccctccg gcgttgtcgc cgatgttttc gggcgacgga
     8881 aaacgctgat tgcaagccag atggcctccc tgatttccgg tacgctgatg attttttcca
     8941 ccggctttgc tacaacggcg cttccgcttg gatgcagtgc gctcagctat aaccttgcat
     9001 ccggcacacg ggaggcactt gcatatgaca gtctgaagca gaacggggac gaagggtttt
     9061 atgcccgttt ttcttctacc gaaatgatgc tgtaccgtat caccaattcc accgcaacgc
     9121 tctgcgcagg tcttgccctg tggctgggat accggcgcgc atatgcgatt gatctcttct
     9181 tcgggttgat tgcgcttggc gtttcattcc gccttgtgga aataaaaacg gacgaaacac
     9241 cagttcatta tcctgttgga caggaaatcg tttcggttgt gcaggaaagc tggcagtttc
     9301 ttgtccgtga gaaaaaagca agaaatatta tgctggtgaa cgcactcatc ggtgccgttt
     9361 ccacgctggt tctcttcttt ttacaggcaa agctgccact caccggcatg aacagcgcgc
     9421 tgcttggacc tgccctcttc gtgatgggac tcggcgcggc actgggatcg cggatgacgg
     9481 catactttca caaataccgc taccgcgcga ttctgctgtt tggcacgatc ggtgtacttt
     9541 tcgcctttct tatgagcttt accggactgc cggcccttat ggtcttcggc ggatttatcg
     9601 gatccttttc cgatgatttt cttgaagtcc ggaccgatat tctgctcaat gacatgatcc
     9661 cgtccaagca gcgggcgact ctgatgtcgg tgaactcgtt tgtcttttct atgattatga
     9721 tcgtcctttc caccgtgttt ggatggatca tgtgatcgtc aaaaatttac tttcattgaa
     9781 aaacgctcat cagttttttt gctgatgagc gtttttttca aaaaatgatc aaacaccgcc
     9841 gctccctgcc agagcgccgt ccggggtctg cgggggtgtg cttcccccct ccacattctc
     9901 caccactttt tctttcttca accacttcca gacatcgcca tatacgcggt acttctcttt
     9961 ctcgttcagt atttcatgcc gcattcccgg atacagcttt cctctgacat tgcggtaccc
    10021 gatcagccgc atatggtcga tggatttttt aaagcgccgc acattcacat aacacggatc
    10081 atccccgccg gacaaaaaca gcaccggcag cttccggttt gtgcaatgcc atccatggaa
    10141 tccatacgtc tccttcagca gatcattcag cgcaaaaaaa gcatcatccg aaaacgtaaa
    10201 tccacagagc ggattttctt catactcccg caccacctcc gggtccgaac agcaccaggc
    10261 gaaccggctc ttctcatccg caaaccgcgc cgcaaacgat ccaaacgaca tcgcctccag
    10321 aagattgctc cgatgctcat ccccgagcac cgccgcttcc gcgtgtccta cagccgcccc
    10381 cagcggccgc agataatttt tgctcggcga accgcacaca atcagcgcat ccagctcctg
    10441 gtcgtgttcc ttcgcgtatg cgcgcacaac cagggatccc atactgtgtc ccatcagaac
    10501 aaacggaaga tccgggtact ccttctttgc ccacttcgtg acaagaaaca gatcctccac
    10561 aagtcctacg ccgcctgcgc cgtacataaa tccgaggtcc tgctccgact tcacactctt
    10621 tccgtgcccg cggtgatcat gaatcacaca cactacaccg tgttccgcca ta
//
LOCUS       DAOMUF010000065        10554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1515_length_10554_cov_2.640632, whole genome shotgun sequence.
ACCESSION   DAOMUF010000065 DAOMUF010000000
VERSION     DAOMUF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1515_length_10554_cov_2.640632"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLN77_05270"
     CDS             complement(<1..1089)
                     /locus_tag="PLN77_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRM43723.1"
                     /translation="MIPERLAALREKMRAYGVDAYLVPTADYHESEYVGTYFKARTYI
                     TGFTGSAGTAVITQDEACLWTDGRYFVQAAKELAGTGVTLMKMGQEGVPTVEEYLQAN
                     TKEGMTIGFDGRVVNEMLGETLEKTLPERFLSYRSDLIGEIWSDRPALSAEPVWILDS
                     KYTGESAADRLAKVREKMKENGAEVHILTALDDIAWMLNIRGNDIPCNPVVLSYLILT
                     ETEGHLFIQEATLNDEVKQYLAGLGISIHPYDAVYDFAASITGKTILLEKAKVNYTIC
                     QSVMGENTIVNVMNPASSLKAVKTPVEMENIRKAHIKDGVAVTRFVYWLKKNIGKIPM
                     DEVSVAEKLESFRKEQEGYIEPSFGTISA"
     gene            1377..4106
                     /locus_tag="PLN77_05275"
     CDS             1377..4106
                     /locus_tag="PLN77_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HRM43724.1"
                     /translation="MHTICLKDNWTLTVLGKNVYDIPETPIETKVPSTVYGTLLEKGL
                     IPDPFYRDNELDALKLMENDFAYETTIVISEEDRKADRLFLHFDGIDTIADVYLNGEK
                     IGSADNMNRVWEYDITDLVAGDAADTVYQVKVVLHSPTKFIAEEEAKCPVGAASDAMP
                     GFPHIRKAACMYGWDWGPRLPDAGLFREASVLAVKTARLAQVFVGQEHHVTGKTVHGN
                     VVDFVRLTADAEIEWMPGVTDGKVKIVMTVTSPDGKTVLTAESGTMDPLTNSAPAKNL
                     TGLTCDLRSNHVQASLTVENPELWWPNGYGAQALYQVKVELVADDILDTWEKKMGLRT
                     ITVNTDVMEDEVFDEHMTGQSPDYDDGDNMIMTNGEKKIIITDKPAKTVKGRNFAYEI
                     NGLQIFAMGADYIPEDNILTRQTRERTDLLLKSAQEANFNSLRVWGGGYFLDDFFYDV
                     CDERGLLIWHDFMFACASYELTDDFEENISAEIRQNMRRLRSHASIALWCGNNEMETQ
                     YETLAWQKSKKQTYDYIHLYEYIIPKIVKEEDPEKFYWPSSPSSGGNYENSNAENVGD
                     THYWGVWHGSEPFTAYRSHHYRFLSEFGFQSFPALQTVKRFTEDGDRNIFSRVMEMHQ
                     RNTAANGKILNYISQTYLYPKNFDELLYCSQLLQADAIRYGVEHFRRFRGTCMGAVVW
                     QLNDIWPVASWASVDYYGNWKALQYAEKKMFAPVLLSCEEHGEIDQKPFVNTLPHPID
                     VSADLHVANETGEPIVGTVKWSLCRPDSSVVKEGAFEVNAPAYGGQWMPHLDFNDQDP
                     LEVHLTYELVVDGNVVSSGSTLFCAPKHYHFADPKLSVSVDGDTVTVTAENFAKSVSV
                     ETENGVLRLDDNFVDMEAGTKTFRILPTADFTAEGIGVSGAYRVRSVYETAER"
     gene            complement(4199..5074)
                     /locus_tag="PLN77_05280"
     CDS             complement(4199..5074)
                     /locus_tag="PLN77_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM43725.1"
                     /translation="MNNLYEYIDPIASPYEAFLSDSTHDLLPIPAHWHYYVELLYVRK
                     GEIEVSLDNVVSTCTKGAVILFPPKCVHSIALARGSSPVQYDVIKFDPNLLPSCKTDE
                     IDLRAMLHGIHAYRCPCIFSPEQTAQMNLFPLFSDILSEYQNKEFGYSMVLTADLRKI
                     VTKFARIWQKNGDFFLAPGVHPSYEIGFDLISEYIDSHYFEELTVEKLAAMCNMSHST
                     FSMNFYRRYNMTCKEYITATRINAAENMLLFSSHDISFIAREVGYTDCSYFIRCYKKL
                     KGVTPKQARKLQLGR"
     gene            5247..6548
                     /locus_tag="PLN77_05285"
     CDS             5247..6548
                     /locus_tag="PLN77_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM43726.1"
                     /translation="MKLIFQFFLPILIGNLFQQLYSFVDSMVVGKGIGDTALAAVGNT
                     SSVHFLIIGFAIGLTGGLGICISHSFGANDTKKLRKEIAMSVYICLAIGIVITTFSLL
                     LMRRLFIFLQTPEDMMTDTLVYFGTILAGSTITIFNNFVMTLLRSVGNSKVPLVSMVL
                     SAIANIVLDLLFVFPLHMGVFGAALATVLAQAISALYCFRHILRAPELLPQKGDWKAD
                     GKILAALTGKGLPVAVMNSVTAIGGMVLQVFVNQMGTAYVAAYAACMKVCGLFEQPGV
                     TVGLALLTFTGQNYGAKKYDRIKSGVWAGVILSILVNLPFAALEIFAPKFLASLMLND
                     PTVISYTCIFLPLTGIALFPLGWLFVFRNACQGMGKTVLPMISGVVEVVMRVGIVQIA
                     VPHFAFRGVAFAEISAWIGAGAMLMVTYLVYQVRLSKGVSK"
     gene            complement(6625..>7137)
                     /locus_tag="PLN77_05290"
     CDS             complement(6625..>7137)
                     /locus_tag="PLN77_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM43727.1"
                     /translation="LRLMNRRYTKEQYLDLVDRIRAKVPDVSLTTDIIVGFPGETEED
                     FLETMDVVRKVRYDSAFTFQYSRRTGTPAAAMEDQIPVDVVKDRFDRLLAEVQEISRE
                     VTARHTHTVQEVLVESINEQDPSLVTGRMSNNTLVHFKGDASLIGTLQNVYLDECRGF
                     YYMGTPVENN"
     assembly_gap    7138..7147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7148..8087)
                     /locus_tag="PLN77_05295"
     CDS             complement(<7148..8087)
                     /locus_tag="PLN77_05295"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRM43728.1"
                     /translation="MDVDITLPAPEAEPQRQYYFMALLKQQLVPERAKKNGKEFLTAC
                     ITTFGCQMNARDSEKLEGILETVGYHIVETEDADFVVYNTCTVRENANLRVYGRLGQL
                     GKYKKKNPGMIISLCGCMMQEPLVVEKLKKSYHFVNLIFGTHNIYRFAEYLVRCMTED
                     RMVIDIWKDTDKIVENLPVDRKYPFKSGVNIMFGCNNFCSYCIVPYVRGRERSRKPEE
                     IVAEIKRLVADGVVEIMLLGQNVNSYGKNLEEPMTFAQLLREVNKIEGLKRIRFMTSH
                     PKDLSEELIDAMRDCEKVCLHLHLPLQSGSSRILKLM"
     gene            8772..9272
                     /locus_tag="PLN77_05300"
     CDS             8772..9272
                     /locus_tag="PLN77_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRM43729.1"
                     /translation="MKVYKKIMNGVAFVEKLILVLSTILIVVLTVGNVLSRKILHQSW
                     SFTEELVVAVFVLITLLAAALAARDGELVNLSLLPDRLSEKARRILLVVNTAFSVAFT
                     AVLFKYGMDKVLTQLANGKRTFVLNWPEWIFWSFVPIGAACMTLHMIEYLIDTFVKAK
                     EEGEEK"
     gene            9269..>10554
                     /locus_tag="PLN77_05305"
     CDS             9269..>10554
                     /locus_tag="PLN77_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRM43730.1"
                     /translation="MISAILFISFFIFLILGIPIGICLGLSSICAILYSGTSLTIVAT
                     NMYSGISKFLLLAIPFFVLSGNIMAKAGISKRLIRFVDTCVGHKKGGIAIVCVIVACF
                     FGAISGSGPATVAALGMVLIPAMIERGGFSAPFATALMATSSSIAIVIPPSIAFVVYA
                     SITGVSIADMFTAGIVPGILMGVALVIVVLLEAKKHNIQPTQKKATAKERWDAFKDAF
                     WGFLMPVIILGGIYGSVFTPTEAAAVSVVYGLFVGIFIYKEIKIKDLWDLMVDSAKTT
                     GGIMLIVASASLFSFVCTKFGIAQAASDLLGSVAHNQFTFLLIVNIIFLIAGCFIDAN
                     SAMYIFIPIMLPVCKALGYDLVAFGIVATVNLAIGQVTPPVGVNLFVAISVKLKKGME
                     VTIQQISKAVMPMIAASVAVLLLITYVPQISTFLPKA"
BASE COUNT         2665 a         2442 c         2667 g         2770 t
ORIGIN      
        1 agcggagatt gttccgaaac ttggctcgat atagccttcc tgctcttttc ggaaggattc
       61 cagtttttcc gcaacggaaa cttcatccat cgggattttt ccgatattct tctttaacca
      121 gtaaacgaaa cgggtcacag caacgccgtc tttgatgtgg gctttgcgga tattttccat
      181 ctcaactggt gttttcactg cctttaagga agaagccggg ttcatcacat tgacaatcgt
      241 gttttcgccc atgacgctct ggcagatcgt gtagttgact ttggctttct ccagaagaat
      301 cgtttttcct gtgatgctgg cagcgaaatc atagacagca tcgtatggat gaatcgaaat
      361 accaagaccg gcaagatact gtttcacttc atcatttaaa gttgcttcct gaataaacag
      421 atggccttcg gtttcggtca gaatgaggta ggagaggaca accggattgc acggaatatc
      481 gtttccgcgg atgttcagca tccaggcgat atcgtcgagt gcggtcagga tatgcacctc
      541 ggcaccgttc tctttcatct tttcgcggac ttttgcaaga cggtcagcag cggactcgcc
      601 ggtatatttg gaatcaagaa tccagaccgg ttctgcagaa agtgctgggc ggtcggacca
      661 gatttcaccg atcaggtcac ttcgatagga gagaaaacgc tccggaagtg tcttttccag
      721 tgtctcaccg agcatctcat tgacaacgcg gccgtcgaag ccgatggtca tgccttcttt
      781 tgtatttgcc tgcaggtatt cctctacggt cggaacacct tcctggccca ttttcattaa
      841 agtaacgccg gttccggcaa gctctttggc tgcctgtacg aagtagcggc cgtcggtcca
      901 gaggcacgct tcatcctggg taataacggc ggttccggca gaaccggtaa agcctgtgat
      961 atacgttctt gctttaaagt aagttcctac atattcagat tcatggtaat cggcggtcgg
     1021 aaccagataa gcgtccacgc cgtaggctct cattttttcg cggagagcag ctaatctttc
     1081 tggtatcata attttttcct catctttctt ttcgaaaaat actgccggca tttttttcag
     1141 gatgccgaca tttttattct atcacatttt ccagatctgt caaagtgatg attgagaatt
     1201 tgtctcactg tttccgaaat tttctaaaat cgtttcgcat ttggagattt ttcctatttt
     1261 tcaagagctt ttttgtacca ttttggttaa aaaagcagta agataaaaat aaagtgaaaa
     1321 gcgtggtgaa actacgcgaa aaagcagaaa ctatgaagaa agggggacat actgtaatgc
     1381 atacaatatg tctgaaagac aactggacat taactgtcct cggaaaaaat gtttacgata
     1441 ttccggaaac tccaattgaa acgaaggtac cgtctaccgt ttacggcacc cttctggaga
     1501 aaggcctgat cccggatccg ttttatcgtg acaatgagct cgatgcactg aaattgatgg
     1561 agaacgattt cgcatacgag acaacgatcg tgatcagtga ggaagacaga aaagcggacc
     1621 gtctgttttt acacttcgac ggaattgata ccatcgcaga tgtttatctg aacggagaga
     1681 aaatcggctc cgcagacaac atgaaccgtg tctgggagta tgatattacc gatcttgttg
     1741 caggtgatgc agccgatacc gtatatcagg taaaagtggt tctgcattcc ccgacaaaat
     1801 tcatcgcaga agaagaagca aaatgcccgg ttggagcagc atccgatgca atgccgggat
     1861 tcccgcatat tcgtaaagca gcctgcatgt acggctggga ctggggtccg cgtcttccag
     1921 atgcaggact gttccgtgag gcttctgttc tcgcagtaaa aactgcccgt ctggcacagg
     1981 tatttgtagg acaggagcat catgtaactg gaaaaaccgt tcatggaaat gtggttgact
     2041 tcgtccgcct gaccgcagat gctgaaatcg agtggatgcc gggtgtaacc gacggtaaag
     2101 taaagatcgt aatgaccgtt acttccccgg atggaaaaac cgttctgacc gcagaaagcg
     2161 gcacaatgga tccgctgacc aattctgcac cggcaaagaa tctgaccgga cttacctgtg
     2221 atcttcgcag caatcatgta caggcttccc ttaccgtaga aaatccggag ctgtggtggc
     2281 cgaacggcta tggtgcacag gcactgtatc aggtaaaggt agagctcgtt gcagacgata
     2341 tcctggatac ctgggagaag aagatgggtc ttcgtaccat caccgtaaat actgacgtga
     2401 tggaagatga agtctttgat gagcacatga ccggccagtc accggattac gatgacggcg
     2461 acaacatgat catgaccaac ggtgagaaaa aaatcatcat caccgataaa cctgcaaaga
     2521 ccgtaaaggg ccgtaacttt gcgtatgaaa tcaacggact gcagatcttc gcgatgggcg
     2581 ctgactacat tccggaggac aatatcttaa cccgtcagac gagagagcgt accgatctgc
     2641 tgctgaaatc cgcacaggag gcaaacttta actccctgcg tgtatgggga ggcggatact
     2701 tcctcgatga ctttttctat gatgtctgcg atgagagagg actcctcatc tggcatgact
     2761 tcatgtttgc ctgcgcatcc tacgagctga cagatgactt cgaagagaat atctccgcgg
     2821 agatccgtca gaatatgcgc agactccgtt cccatgcaag tattgctcta tggtgcggaa
     2881 acaacgagat ggaaacccag tacgaaacgc tggcatggca gaaatctaag aaacagacgt
     2941 atgattacat ccatctgtat gagtacatca ttccgaaaat cgtcaaagaa gaggatccgg
     3001 agaaattcta ctggccgtca tccccgtctt caggcggaaa ttacgaaaac agcaatgcag
     3061 agaacgtcgg cgatacccat tactggggtg tatggcacgg aagcgagccg ttcaccgctt
     3121 atcgttccca ccattaccgt ttcctgtccg agttcggatt ccagtccttc ccggcactgc
     3181 agactgtaaa acggtttacc gaagacggcg accgcaacat cttctcccgt gtcatggaaa
     3241 tgcaccagag aaatacggct gccaacggaa agatcctgaa ctacatttca cagacatacc
     3301 tgtatccgaa gaacttcgac gagcttctgt actgctcaca gcttcttcag gccgatgcga
     3361 tccgttacgg cgttgagcat ttccgtcgct tccgcggtac ctgcatgggt gctgttgtat
     3421 ggcagctgaa cgatatctgg ccggtagcat cctgggcaag cgtggactac tatggaaact
     3481 ggaaagccct gcagtacgca gagaagaaga tgtttgcacc ggtcctgctt tcctgcgagg
     3541 agcacggaga gatcgatcag aaaccgtttg tcaacacact gccgcatcca attgatgtca
     3601 gtgcagacct tcatgttgcc aatgagaccg gtgagccgat cgtcggaacc gtaaaatggt
     3661 ccctgtgccg tccggattct tccgtagtca aagagggagc tttcgaggtg aatgcaccgg
     3721 cttacggcgg acagtggatg ccgcatcttg atttcaacga tcaggatccg ctggaagttc
     3781 atctgaccta tgagctggtt gttgatggaa acgtagtatc ttccggatca actctgttct
     3841 gcgcaccgaa gcattaccat tttgcagatc cgaagctttc tgtatcagta gacggcgata
     3901 ccgtaaccgt aaccgcagag aactttgcaa aatctgtcag cgtagagact gagaacggtg
     3961 tgcttcgtct ggacgataac tttgtggata tggaagccgg aacaaagacc ttccgcattc
     4021 tgccgaccgc agactttacc gcagagggaa tcggcgtatc gggcgcatac cgcgtaagaa
     4081 gcgtttatga gactgcagag agataaggaa aaattttaaa gaaatacata aaaaagaaag
     4141 agcgtgcagc aaatgcctgt gcgttctttc tctgaaaaaa ttcgaaaaca gatgatcgtt
     4201 accgtccaag ctggagtttt cgtgcctgct tcggcgtgac gcctttcagt tttttgtagc
     4261 agcggataaa gtaactgcag tccgtataac cgacctcccg cgcgataaac gagatgtcgt
     4321 ggctggaaaa caaaagcata ttttcggctg cattgatccg ggttgctgta atgtattcct
     4381 tgcaggtcat attgtaccgg cggtagaaat tcatggaaaa ggtggagtgc gacatattgc
     4441 acatcgcggc aagtttttct acggtcagct cctcaaagta gtgggaatcg atgtattcgg
     4501 aaatcaggtc aaacccgatt tcataagaag gatgaacgcc gggagccaga aagaaatcac
     4561 catttttctg ccagatccgc gcaaacttgg tgacgatttt ccgaagatcg gcagttaata
     4621 ccatcgaata accgaattct ttattctgat attcggaaag aatatcggaa aaaagcggaa
     4681 aaagattcat ttgggccgtc tgttccggag aaaagataca gggacagcgg taggcgtgaa
     4741 tcccatgcag catggcgcgc agatcgattt catccgtttt gcaggacggc agaagattgg
     4801 gatcgaattt gatcacatcg tactgaactg gagaagaacc tctcgcaaga gcgatggagt
     4861 ggacgcattt gggcggaaaa agaatgacag cgcccttcgt gcaggtggat acgacattgt
     4921 caagggaaac ttcgatttcg cctttgcgga cgtagagaag ttctacataa taatgccagt
     4981 gcgccggaat cggcaggaga tcgtgggtgc tgtcagaaag aaatgcctca tagggggaag
     5041 caattggatc gatgtattcg tataaattgt tcatgccagt gatgtaaaaa cctttcgtct
     5101 aagttatgct ctattatagc cgcattttgc ggtcttgaca agacgagggg gaatcaataa
     5161 agaaacagga aattctaaaa gagaaaggac agggtagaaa aaataccatg cagaacacaa
     5221 caaatgatat gacaagaggg aacagcatga aactgatttt tcagtttttc ctgcccattc
     5281 tgatcggaaa tctgttccag cagctttaca gcttcgtgga ttccatggtt gtcggaaaag
     5341 gaatcggaga tacggcgctt gccgcagttg gaaacacaag ctccgttcat tttctgatta
     5401 tcggctttgc catcggactg acgggtggcc ttggcatctg tatttcgcat tcttttggtg
     5461 caaacgatac aaaaaagctg cggaaagaga ttgccatgtc ggtctatatc tgccttgcca
     5521 tcggaattgt gatcacaacg ttcagcctgc ttctgatgcg gcggctgttt atctttctgc
     5581 agacgccgga ggatatgatg acggatactc tcgtgtattt cggaacaatc ctggccggaa
     5641 gcaccatcac gattttcaat aactttgtga tgacactgct ccgctcggtc ggaaacagca
     5701 aggttccgct cgtctccatg gtgctttctg ccattgccaa tattgtgctg gatcttctct
     5761 ttgtgtttcc gcttcacatg ggtgtatttg gagcagcact tgccacggtt ctggcgcagg
     5821 cgatttccgc gctgtactgc ttcaggcaca tcctccgtgc accggaattg cttccacaga
     5881 agggggactg gaaagcggat ggaaaaattc ttgccgcgct gaccggaaaa ggtcttccgg
     5941 ttgcagtgat gaattctgtt actgccatcg gaggaatggt gcttcaggtc tttgtcaacc
     6001 agatgggaac cgcctatgtg gctgcgtatg cggcatgcat gaaggtctgc ggtctgtttg
     6061 agcagccggg tgtgaccgtc ggactggcgc ttctgacctt taccggacag aactatggag
     6121 caaagaagta tgacagaatc aagagcggcg tatgggccgg tgtgatcctg tcgattctgg
     6181 tcaatctgcc gtttgcggcg ctggaaattt tcgcgccgaa attcctggca tctctgatgc
     6241 tgaacgatcc gaccgtcatt tcctacacct gtatctttct tccgctcacc ggaatcgcat
     6301 tgtttccgct tggatggctt ttcgtattcc gaaacgcctg ccagggcatg ggaaaaacgg
     6361 tactgccgat gatttccggt gttgttgaag ttgtgatgcg tgtcggcatc gttcagattg
     6421 cggttccgca ctttgcgttc cgcggtgtcg cattcgctga gatttccgca tggattggcg
     6481 cgggagcgat gctgatggtt acgtatctgg tgtatcaggt gcggctgtca aaaggtgttt
     6541 cgaagtaaaa ataaaaatcc cggattctgg atacctgccg taaaagcagg aaccgaaaat
     6601 ccgggatttt tgtttttgaa agatttagtt gttttccacc ggtgttccca tgtaatagaa
     6661 accgcgacac tcatccagat atacattctg cagcgtgccg atcagggaag catcaccttt
     6721 gaaatgaacg agcgtattgt tactcatacg gccggtcaca agggacggat cctgttcatt
     6781 gatgctctcg acgagaactt cctgaactgt gtgggtatgt cttgccgtta cctcacggga
     6841 gatttcctgt acttcagcaa gaagacggtc gaaacggtcc ttcacaacat cgaccggaat
     6901 ctgatcctcc atagcggcag ccggtgttcc tgttcttctg gaatactgga aggtaaaggc
     6961 gctgtcgtag cggaccttac gtaccacatc catcgtttcg aggaaatctt cctctgtctc
     7021 gccagggaaa ccgacgatga tatcggttgt cagggagaca tccggtactt tcgcgcggat
     7081 gcggtcaaca aggtcaagat actgctcttt cgtgtagcgg cggttcatca ggcgcagnnn
     7141 nnnnnnntca tcagctttaa gatgcggctg cttccggact ggagcgggag atgaagatgg
     7201 aggcagacct tttcgcagtc gcgcatcgca tcgatcagct cctcagagag atccttcggg
     7261 tgggaggtca tgaaacggat gcgcttcagc ccttcgattt tattaacctc gcgcagaagc
     7321 tgggcaaacg tcatcggctc ctcgagattc tttccgtaag agttgacgtt ctgaccgagc
     7381 agcatgatct ccacaacgcc gtctgcaacg aggcgtttga tctctgcaac gatttcctcc
     7441 ggtttccggc tgcgctcgcg gccgcgcaca tacggtacga tacagtagct gcagaagttg
     7501 ttgcaaccga acatgatatt gacgccggat ttgaacggat atttccggtc aaccggaaga
     7561 ttttctacga tcttgtcggt atctttccag atatcgatca ccatgcggtc ctcggtcata
     7621 caacggacaa ggtattcagc gaagcggtag atattgtggg ttccgaaaat caggttgacg
     7681 aaatgatagc tttttttgag cttttcgaca acgagcggct cctgcatcat gcatccgcag
     7741 agggagatga tcatgcccgg atttttcttt ttgtatttgc caagctgtcc gagacggccg
     7801 tagacgcgaa gattggcgtt ttcacggacg gtacaggtat tgtaaacgac aaagtcagca
     7861 tcctcggtct ccacaatgtg gtagccgacg gtttccagga ttccctcgag cttttcagaa
     7921 tcgcgggcat tcatctgaca tccgaatgtt gtgatacagg cagtcaggaa ttctttgccg
     7981 ttttttttcg cgcgttccgg gacgagctgc tgttttaaga gcgccataaa gtaatactgg
     8041 cgctgcggtt ctgcttccgg agccgggagt gtgatatcaa cgtccatcag gtcgatgccg
     8101 gaaacctctg catccggaat cgttctaaga tctattttca tagttgctgt tcctcttttc
     8161 tgggtagtaa aaatcgtggc agaatgccga gaaaaaagac ccgtcgcatc ggatcttatc
     8221 tgtctgttat tttatcgtat ctgtgggaaa atagcaagga aaaaaggtgc tgcaatcgaa
     8281 acagaacagc aaaccgaaaa ttttacggtt tttcaaaatt ataacaaaat ttcagaacca
     8341 attcttgaca aatgacaaaa atcgcgctaa aattacagcc gtcgtgtaag ataaattctg
     8401 ggaagagaaa aagtaaatgc ggaaaaagcg tttgcagaga gctgtcggag aaggtgcgag
     8461 acagcagcgt gtccggattg aatatcatct cggagaaggg aaacccaaag aactgttttt
     8521 attgtttcat taagataaag acaggattcc agtaggtttt cacgggagtt tcccgttaca
     8581 gaaaccgcgt atgcttgtgc gtgtattcgt acttgtttca aaggagagac aagggtggtt
     8641 tgcggaaaaa atatggtccg tccctggtta tcgtggggcg gcttttttta tgcagaaaca
     8701 ccggtaatac agcctggcaa gaaacaaatc ctgctgcaaa acacgcgcag acaggaaaag
     8761 ggaggaaaca tatgaaagtg tacaaaaaaa tcatgaacgg cgtggcattt gtcgagaaat
     8821 tgattcttgt gctgtccacg atcctgattg ttgtgcttac ggtcggaaat gtcctgtccc
     8881 gtaagattct gcatcaatcc tggtcattta ccgaggaact cgttgttgcg gtatttgtcc
     8941 tgattacact tctcgctgca gcgctcgctg cccgtgacgg agagctggtc aatctttctt
     9001 tactgccgga ccgtctttca gaaaaagcga gaagaattct gctggtggtc aacacggctt
     9061 tcagtgttgc atttaccgca gtcctgttta aatacggcat ggacaaggtt ctgacccagt
     9121 tagccaacgg aaagagaacc ttcgtgctga actggccgga atggattttc tggtccttcg
     9181 taccgatcgg agccgcctgc atgacgctcc acatgatcga gtatctgatt gatacctttg
     9241 taaaagcgaa agaggaggga gaagaaaaat gatcagtgca attcttttta tttcattctt
     9301 tattttcctg atccttggta ttccgatcgg catctgcctc ggtttatcct cgatctgcgc
     9361 aattttatat tccggcacat cgctgacgat cgttgccacc aacatgtatt ccggcatcag
     9421 taaattcctg ctgcttgcca ttccgttctt tgtcctgtcc ggaaatatca tggcaaaggc
     9481 gggtatctcc aaacggctga tccgtttcgt tgatacatgt gtcggccaca aaaaaggcgg
     9541 tatcgccatc gtgtgcgtta tcgttgcctg cttcttcggc gctatttccg gttccggtcc
     9601 ggcaactgtt gcagctcttg gtatggttct gattcctgcc atgatcgagc gcggcggctt
     9661 ctccgcaccg tttgccacag cgctgatggc gacatcgagt tcgatcgcaa tcgtcattcc
     9721 gccgagtatc gcattcgttg tttacgcatc catcaccggc gtatccattg cagatatgtt
     9781 caccgcaggt atcgttcctg gtatcctgat gggtgttgct cttgtaatcg ttgttcttct
     9841 tgaggcaaag aaacacaaca ttcagccgac gcagaagaag gcaacggcaa aagagagatg
     9901 ggatgccttc aaagatgcat tctggggatt cctgatgccg gttattattc ttggcggtat
     9961 ctatggaagc gtattcactc caacagaagc agcagccgta tccgttgtat acggtctgtt
    10021 tgtcggaatc tttatctaca aagaaattaa gatcaaagat ctgtgggatc tgatggtaga
    10081 ctctgcaaag accaccggcg gtattatgct catcgtagca agtgcttccc tgttttcctt
    10141 tgtctgcacc aaattcggta ttgcgcaggc agcttccgat ctgcttggaa gcgttgcaca
    10201 taatcagttc acattcctgc tgatcgtcaa tatcattttc ctgattgcag gatgctttat
    10261 cgatgcaaac tctgccatgt atatttttat cccgattatg cttccggtct gcaaagccct
    10321 tggctatgac ctggtagcat tcggtatcgt ggcaaccgtc aacctggcca tcggacaggt
    10381 aactccaccg gttggtgtca acctgtttgt tgccatttcc gtaaaactga aaaaaggaat
    10441 ggaagttacc attcagcaga tttccaaagc cgttatgcca atgatcgcag catcggtagc
    10501 agttctgctt ctgattacgt atgttccgca gatttccacg ttccttccaa aagc
//
LOCUS       DAOMUF010000066        10347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1571_length_10347_cov_4.144967, whole genome shotgun sequence.
ACCESSION   DAOMUF010000066 DAOMUF010000000
VERSION     DAOMUF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1571_length_10347_cov_4.144967"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..1928)
                     /locus_tag="PLN77_05310"
     CDS             complement(306..1928)
                     /locus_tag="PLN77_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM43731.1"
                     /translation="MKFKKSIAMAMSVLMAAGMLAGCGSNGSTSSSTASGSASSASGE
                     VSTDGVTISIFDKNSGSNTFDDPVAKAIEEKTGVKIQVENPTGDPLEKLNLMLTGQNY
                     PDIVLMDRGNDIVNRYIEAGALLPLNDLIDEYGPDVKEMYGETLNKSRYTDGKNYYLN
                     NWYGEDPDPVAGVLMRYDYLCEIVGKDRADSDEPFTQEEYIDICKKFKELHPTIDGKE
                     SIAITFDAESKNYDGTLKGMYGLKTYYQDGDNLEHVAKAPNFKEMMSFANELYTEGLL
                     DKEWVVNKKNQWTQKLSAGNVFSTFASYWDTDAANTALASSVGEDAKFYSYKVLGNGI
                     SADETTYSGRSTLGWDAIAITKNCKNPEAAMKMINYLASEEGQYLLMWGIEGTNWNME
                     DGKHVPNDDLIEGFQTDFDKTKLDTGVRKWTWFVKNGNGTDGTPYDVTQYKVKETRQV
                     AMNHFGENDRWDTAEFAGLTPAGSTPDGLKWQKIQDIYDQEYPKIVNADSHDAAMEEY
                     DKMISEMNDAGLEDVEKVITQNYQERMKLWNE"
     gene            complement(1950..2849)
                     /locus_tag="PLN77_05315"
     CDS             complement(1950..2849)
                     /locus_tag="PLN77_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM43732.1"
                     /translation="MREKAIKIKRKRCASDWFLDIFKVVFLVLVTVICVYPFWNIFVV
                     SINDATDALRGGLYFLPRKLSLASYKDILGRATFLHGILITVLRTAIGTPLAVLVTTA
                     LAYVLSRKELLFRKQLNLFFIFTMYFGGGMVPYYMVLKSLGMLNNFIVFIFPNLVSVY
                     NMILVKNYIEGMPAEIFESAKIDGANDLTVFFKMVIPLSKPIIMTIALFVAITHWNSW
                     FDAYLFTNAQSLKTMQSILVEILNQYQTSSANQAANQASQTITPDSIRMAATMIATIP
                     IIMVYPFIQKYFVKGIMLGSVKS"
     gene            complement(2861..3847)
                     /locus_tag="PLN77_05320"
     CDS             complement(2861..3847)
                     /locus_tag="PLN77_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM43733.1"
                     /translation="MNKKKKEGEGMKKENVHAKHKKSLAERFWKQKYLFLLLLPSLVW
                     VILICYAPMTGLYMSFINYTPQGSGYFADMANSQFVGLDWFKYFFSTDFTKIMRNTLA
                     TSLLTLLFSFPLPIILALCLNEVRNQKAKKFVQTASYIPYFISWVIAANIFLTFLSSD
                     GVINNLLKFFGLTDESILFFQKGPYFWWIIAIANAWKVLGYNAIIYMAAISGISQDMY
                     EAADVDGATRMQKILHITLPALKPTIMILLILAIGGVLNTGYEQQLLMSNDAILNYSD
                     VLDTYAYRYGLKNGMYSYGTAVGFFKSAVSFILVISANALSKKFSDDNTALF"
     gene            complement(4100..5812)
                     /locus_tag="PLN77_05325"
     CDS             complement(4100..5812)
                     /locus_tag="PLN77_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM43734.1"
                     /translation="MNIVKWYKNRSFQFKLVIGYLVLALIPMLCVTWYSYGKTRNVLL
                     TEAYQSAEQEAERIEKNFSTMVEPYETILDVLYVDQMLSGYLFQDYSNDSYEDMFYYI
                     DKKLSEICLMNAGIYKICFYSNNETLPQDNYYFYSMQDLDRRECVLTFDAIGETVFCG
                     TSGDGKAFHMNRLMNFYPQGGMKGVLSLQIENQQIQPLLETINSTDEIYLVDQKGYIL
                     AASEPEMAGLPIRTRMPEEDLEQKAQIEFERDGISKIGNIQSGVFGTKILVISDKETV
                     LKDAKAVSRRMMFLILCSAAMVFLCIICYTRWISARVQKVVYAAKRMGQGEFDYRLEN
                     MGEDEIGQIGLAFNQLSDQIQELIRENYEKKIRIQTSELNLLQEQINPHFLYNALAVI
                     SALAMREGGKQTMQAVKNLSSFYRISLNKGKQVLSIQEEVELLQSYLKIQQMRFRDSV
                     QVEYEIAREVLTYRTIKLLLQPLVENAIHHARKEEEVLHIAVRIQKEERDVVFQVTDD
                     GCGIEAEKLIKLRSSLRRSEEGYGLRNVANRVRLAYGENYGVRIESQEGYGTTVSVRI
                     PVNE"
     gene            complement(5790..7286)
                     /locus_tag="PLN77_05330"
     CDS             complement(5790..7286)
                     /locus_tag="PLN77_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM43735.1"
                     /translation="MYRVLLVEDEEIELETLRDYIDWKKNGISRVFTARNGRRALECL
                     EENMADIVITDIQMPGMSGIELAETIVRRGYPCKIVFLTGYDDFSYIHSAFQVNAVDY
                     LLKPFTIEEVEACLKKVRSELEKREIADWSRKTAAKQLLEMALREKQETELLRKNFRG
                     VFGEELEECRFGIIAVYGKTEENICSQIQEKYKGIRYISKTERISMLLLAGYLSVKDT
                     AFQIWNDLKEDAPRAVGWCSGAWTADCLYEKAEKLRNCCVLAFHMDPPELFCADEKET
                     EEEKAYHFREQKERREEICRLVSNGKKQETLQTMKDYFQQLKGLAPKTFAGEVYNLYM
                     YLWNRLVLSDELLENWMEAEKILRENEIFEANNSYQMREKMKQYLERMLAFFEEQNQN
                     PNYYAVYQVKTYLQEHCSESADIEKLAAEVGLSPNYLRSLFKEATGKTILEYNTEMRL
                     QRAAELLKNKKNKVREVSLAVGYENVSYFGVVFQKRFGVTPNEYRKMV"
     gene            complement(7353..8984)
                     /locus_tag="PLN77_05335"
     CDS             complement(7353..8984)
                     /locus_tag="PLN77_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRM43736.1"
                     /translation="MKREWWHSLTVYQIYTRSFCDSNGDGIGDFGGILQKLDYLQELG
                     VGAIWLSPFNDSPNYDNGYDVSDYYKVMEEAGTMEELEELIAACEKREIVVMMDLVLN
                     HSSHLHPWFLEARKDRNSKYHDFYIWKEGTKEQPPEGGGAFFGGSTWEWVPEVQEYYY
                     HSFSVMQPDLNWKNPSLRKELYRMIQFWMDKGIRGFRLDAIDNIVKDGHGGNDTHSEQ
                     IHTYLMEMNQNTYGKSEQILTVGETGGATVEMAQQYSDPESQELSMIFQFELMGIDGI
                     RSGNWDPKPYTLPQLKQLFEKWQTGLEEKGWNSLFWGNHDFPRVVSRFGNDREPYREK
                     SAKMLAVLLHGMKGTPYIYQGEEIGMTNVSGLRIEDYQDIESVNFAEDRKKEGWEEEK
                     IRTYLARNSRDHARTPMQWNAEKHAGFTAGTPWMAENQNYEEINVENSRKNPDSLFYF
                     YQKLIALRRKNDTLVYGDFRLIEEENPDIFAYERNLGEKKLIVLCNFRDTAAEMKARY
                     DLTKGTVLIHNDTESLTKEVWKLQPYEAYMIELLQ"
     gene            9086..10048
                     /locus_tag="PLN77_05340"
     CDS             9086..10048
                     /locus_tag="PLN77_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM43737.1"
                     /translation="MKKERSKELFSPESCSPVLSGGIRDFTVHGTEPVPLHIYHQDYA
                     HGGLSVPFHWHKELEWIWVEKGTLTLTLKTETRTISEGTFLFINSHELHQLCSIGTTP
                     SVHHALVFLPEILSCAYLDSCEADWIRPLLSHQLLLPAFPLEELSAAEASSQTQKTFV
                     LTLSELFQEILYAYDTKPIGWFLLIKSDMYRILALLAQYQLFLHPEPLCTRKKERSDQ
                     CKKLLRYIQDNYDSKIRLSDLAAILNMNPQYFCRFFKTHFHTTPISYINQYRVTQAAD
                     LLTGSSLSILEIALHTGFESPSYFASIFRRTFGMSPEEYRKAMP"
     gene            10199..10270
                     /locus_tag="PLN77_05345"
     tRNA            10199..10270
                     /locus_tag="PLN77_05345"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10232..10234,aa:Glu,seq:ttc)
     gene            10274..10345
                     /locus_tag="PLN77_05350"
     tRNA            10274..10345
                     /locus_tag="PLN77_05350"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10306..10308,aa:Cys,seq:gca)
BASE COUNT         2579 a         2636 c         1832 g         3300 t
ORIGIN      
        1 tccggagatg caaaatcccc gaacgcttcc ttctgcggcg caaagtccgt ctttcccata
       61 tgtcctgtcg tcccactcat aaatccccag atcatttcca ttgcggttaa ctgttctttc
      121 gctgccatgt acgatgcctc ctgtctcatg cggttggatt tccctgtttt tccagttttc
      181 ctcattatat ccagattcct tgaaaatcgc aatgcttctt ctgataaaaa agaaccgaca
      241 tagaaatctg tatttcgatt tctgccggtt cttttccgtg tgtgattttt ttattttact
      301 ttagcttact cattccagag tttcatacgc tcctgataat tctgtgtgat aactttttct
      361 acatcttcca gtcctgcgtc attcatttcg gaaatcatct tgtcgtactc ttccattgcc
      421 gcatcgtggc tgtctgcatt cacaatcttc ggatattcct gatcgtagat atcctggatt
      481 ttctgccatt tcaagccatc cggagtgctt cctgccgggg taaggcctgc aaattctgcg
      541 gtatcccatc tgtcgttctc accaaagtga ttcatagcca cctgccgggt ctcttttact
      601 ttatactgtg ttacatcgta tggagttccg tctgtaccat ttccgttctt tacaaaccag
      661 gtccatttgc ggacgccggt atccagtttt gttttgtcaa aatccgtctg gaatccttca
      721 atcagatcat cgttcggaac gtgttttcca tcttccatat tccagttggt tccctcaata
      781 ccccacatca gaagatactg tccttcttcg cttgcaaggt agttgatcat cttcatggct
      841 gcttccggat ttttacaatt cttggtgatc gcgattgcat cccagccaag tgtacttctt
      901 ccggagtaag tagtttcatc tgcactgatt ccgtttccaa ggactttata ggaatagaat
      961 tttgcatctt ctcctacaga agaagcaagt gctgtatttg ctgcatcggt atcccagtag
     1021 gaagcaaagg tactgaatac gttgcctgca gacagtttct gcgtccactg atttttcttg
     1081 ttgacaaccc actctttgtc cagaaggcct tcggtataca gttcattggc aaaggacatc
     1141 atctctttga agtttggtgc tttggcaaca tgttccagat tgtccccatc ctgataatat
     1201 gttttcagac catacattcc ttttaatgtt ccatcgtagt ttttggattc tgcatcaaag
     1261 gtaatggcaa tactctcttt tccgtcaatg gtcggatgaa gttctttgaa tttcttacaa
     1321 atgtcaatat actcttcctg tgtaaatggc tcatcgctgt ctgcgcggtc ttttccaacg
     1381 atttcgcaca gataatcata gcgcatcaga acaccggcta ccggatccgg atcttctccg
     1441 taccagttat tcagatagta attctttcca tcggtataac gactcttgtt taaggtttcc
     1501 ccgtacatct cttttacatc cggaccgtac tcatcgatca gatcattcaa cggaagcagt
     1561 gcgcctgcct caatgtaacg gtttacaata tcatttccac gatccatcag gacgatatcc
     1621 gggtaattct gaccggtcag catcaggttc aatttttcca gcggatctcc ggttggattt
     1681 tctacctgaa ttttgacacc tgttttttcc tcaattgctt ttgcaactgg atcatcaaag
     1741 gtattgcttc cggagttttt gtcaaagata gaaatcgtaa ctccgtctgt tgatacctcg
     1801 cccgatgcag atgatgcact gccggatgca gtacttgatg atgtactgcc atttgatccg
     1861 catcctgcaa gcattcccgc tgccatcagg acactcatcg ccattgcgat actttttttg
     1921 aacttcattt tcttttcctc cttctatcat cagctcttta cagatccgag catgattcct
     1981 ttaacaaaat atttctgaat aaacggataa accatgataa tcggaatggt cgcaatcatc
     2041 gtagcagcca ttcgaatgct gtccggtgta atggtctgtg acgcctgatt tgccgcctgg
     2101 ttcgctgatg aagtctgata ctgattcaga atttcaacca aaatggactg cattgttttt
     2161 aaagactgtg cattcgtaaa caaatatgca tcgaaccaag aattccaatg ggtaattgca
     2221 acaaacagtg caatagtcat aataatcggc ttggacaatg gaatcaccat tttaaagaaa
     2281 actgtcagat cgtttgctcc gtcaattttc gccgattcaa aaatctctgc cggcattccc
     2341 tctatataat tctttaccag aatcatatta taaacactta ccaggttcgg gaaaataaat
     2401 acaatgaaat tattcagcat tccaaggctc tttaatacca tataatacgg aaccattccg
     2461 ccgccaaaat acatggtgaa gataaagaac aggttcagct gtttccggaa cagcagctct
     2521 tttctggaaa gcacataggc aagtgctgtc gtgacaagga ccgccagcgg tgtaccaatt
     2581 gcagtacgaa gtacagtgat cagaatacca tgcaaaaatg tggcacgtcc cagaatatct
     2641 ttataacttg ccagactcag ttttctcggc agaaagtaga gcccgccgcg cagtgcatcg
     2701 gttgcatcgt taatggatac cacaaaaatg ttccagaacg gatacacaca gatcacagta
     2761 actaacacca gaaaaaccac tttgaaaata tcaagaaacc agtcactggc acaacgtttt
     2821 cttttgattt taattgcttt ttctctcatt ttccttcctc ctaaaacagt gccgtattat
     2881 catcactgaa tttcttcgaa agtgcatttg cactgatgac cagaatgaag gagactgcgg
     2941 atttaaagaa tcctactgca gtaccgtaag aatacattcc gtttttcaga ccatagcggt
     3001 atgcatacgt atcaagtaca tcagaataat ttaaaatagc atcattagac atcaacagct
     3061 gctgctcata tcctgtattc agaacacctc cgattgccag aatcagaaga atcataatgg
     3121 tcggctttaa tgccggaagt gtgatatgca gaatcttctg cattctcgtc gcaccatcca
     3181 catccgctgc ttcatacata tcctggctga tgccggagat tgccgccata tagataatcg
     3241 cattgtatcc aagtaccttc cacgcatttg cgatcgcgat gatccaccag aaatatggtc
     3301 ctttctggaa aaacagaatc gactcatcgg ttaatccgaa gaattttaac agattgttga
     3361 tgacgccgtc tgaagaaagg aatgtaagga aaatgtttgc cgcaataacc caggaaataa
     3421 aatagggaat ataagatgcg gtctgtacaa atttctttgc cttctggttt cgaacttcat
     3481 tcaggcaaag cgccagaatg attggcagcg ggaatgagaa taacagtgtc agtaagctgg
     3541 ttgccagtgt gtttctcatg atttttgtga aatctgtgga aaagaaatac ttgaaccagt
     3601 caagaccgac aaactgcgaa ttcgccatat ccgcaaaata gccgctgccc tgcggcgtgt
     3661 agttgatgaa ggacatatac agtcctgtca ttggtgcata gcagatcaga atgacccata
     3721 ccaacgatgg aagcagcagt aaaaacagat atttctgttt ccagaaccgt tccgcgaggc
     3781 tttttttgtg ctttgcatgc acgttttctt ttttcatgcc ttctccttct ttcttctttt
     3841 tgttcatctt gtacactctt gctgtctatg tttccatttt aatggatagt ctgtcctgca
     3901 acaatctcac ttttttatga tttctatctc gatttaacgg agttttatag ttctcttttt
     3961 tattctgttg ttttcttacg atttctgtca atatctaacg aaacagaaac cggctgacgg
     4021 gcaaaatcac ctttatcagc cggtttctgt ttccttttct atgcaaacaa tacgaagtta
     4081 tttctgcttt gttgattttt tattcgttta ctggaattct gacagaaact gtcgttccat
     4141 atccttcctg gctttctatt cggacaccat aattctctcc ataagccaga cgaaccctgt
     4201 ttgcaacatt tctcagtcca tatccttcct ctgagcgccg cagagaactt cgcagtttaa
     4261 tcagtttttc cgcctcgatt ccacatccat catctgtcac ctggaatacc acatcccttt
     4321 cttctttttg aattctgacc gcaatgtgga gaacttcttc ctcttttctc gcatggtgaa
     4381 tggcattttc aaccagcggc tgcagaagaa gtttaatggt tcgatatgtc agtacttctc
     4441 ttgcaatctc atactccacc tgtacgctgt cccgaaaacg catctgctgg attttcagat
     4501 agctctgcag cagttccact tcttcctgga tggaaagcac ctgttttccc ttattcaggg
     4561 aaatccggta gaagctggac aaatttttga ccgcctgcat cgtctgtttt ccaccttccc
     4621 gcatcgccag tgctgaaata accgcaagtg cattgtaaag aaagtgcgga ttgatctgtt
     4681 cctgcaacag attcagttcg ctcgtctgaa tccggatttt cttttcgtaa ttttcccgaa
     4741 ttagctcctg aatctgatca ctgagctgat taaacgccag cccgatctgt ccgatctcat
     4801 cttctcccat attttccaga cggtaatcaa attctccctg tcccatccgt tttgctgcat
     4861 acaccacttt ctgcacccgc gcggaaatcc atcttgtgta acagataatg cagagaaata
     4921 ccatagccgc gctgcagagg atcaggaaca tcatccttct tgaaacagct tttgcgtctt
     4981 ttaaaaccgt ctccttatcc gaaatcacaa gaatttttgt tccgaaaact ccgctctgga
     5041 tatttccgat ttttgaaatg ccgtcacgct caaactcaat ctgcgctttc tgctccagat
     5101 cctcttccgg cattctggtc cggatcggaa gacctgccat ttccggttcg gaagcagcca
     5161 gaatatatcc tttttgatcc accagataaa tttcatcggt ggaatttatt gtttcgagga
     5221 ggggctgtat ctgctgattt tcaatctgta acgacagtac gcccttcatc ccgccctgtg
     5281 gataaaaatt catcagacga ttcatatgaa atgcttttcc gtctccagat gtcccacaga
     5341 ataccgtttc ccctatcgca tcaaatgtca gcacacattc cctccgatct agatcctgca
     5401 tggaataaaa ataataatta tcctgcggaa gcgtttcatt gttgctgtaa aaacagattt
     5461 tgtaaattcc tgcattcatc agacagattt cgctgagctt cttatcaata tagtaaaaca
     5521 tatcttcata agaatcgttg ctgtaatcct gaaacaaata tccgctcagc atctgatcca
     5581 catacagcac atccagaatt gtttcgtaag gctcgaccat cgtagaaaaa tttttttcaa
     5641 ttcgttctgc ttcctgttct gcactctgat atgcttcagt aagaagcaca ttccttgttt
     5701 ttccataact gtaccaggta acacacagca ttggaatcag tgccagtacc agatagccga
     5761 tcaccagttt aaactgaaaa ctccgatttt tataccattt tacgatattc atttggtgtc
     5821 actccaaatc ttttctgaaa aacaacgcca aagtaagaaa cattttcata tccaaccgcc
     5881 agactcactt cccgtacttt atttttctta ttctttaaca gttccgccgc cctctgcaga
     5941 cgcatttccg tattgtattc caaaatcgtt tttcctgttg cctctttaaa caggctcctc
     6001 agataattcg gagacaaacc gacctccgcg gccagcttct cgatatctgc gctttccgag
     6061 caatgctcct gaagatatgt ttttacctga taaacggcat agtaatttgg attttgattc
     6121 tgttcttcaa aaaatgccaa catccgttcc agatactgct tcatcttttc ccgcatctga
     6181 taactgttgt ttgcttcaaa aatttcattt tctctcaaaa ttttttctgc ttccatccag
     6241 ttttccagca gttcatcgct caaaacaaga cggttccata gatacatata gagattgtaa
     6301 acttctcccg caaatgtttt cggcgcaagc ccctttagct gttgaaaata atctttcatg
     6361 gtctgcagcg tttcctgctt tttcccgttg ctgaccagac gacagatttc ttctctccgc
     6421 tccttctgtt cccggaaatg atatgctttt tcctcttccg tttctttttc atctgcacag
     6481 aaaagttccg gtggatccat atgaaatgcc agaacacaac agttccgaag tttttccgcc
     6541 ttttcgtaca ggcagtcagc tgtccatgct ccactgcacc atccaacggc tctcggtgcg
     6601 tcttctttca aatcattcca gatctgaaat gccgtatctt tcaccgacag ataccccgcc
     6661 agcagcagca tactgatccg ttccgttttg ctgatgtaac ggatgccctt gtatttttcc
     6721 tgaatttggc tacagatgtt ttcttccgtt tttccataaa ccgctatgat tccaaacctg
     6781 cactcttcca gttcttctcc aaatacacca cggaaatttt tccgtaaaag ctctgtttcc
     6841 tgtttttccc gcaaagccat ttccagcaac tgcttcgccg ccgttttccg cgaccagtct
     6901 gctatctctc ttttctccag ttcacttctc acttttttca gacatgcttc tacttcttca
     6961 atcgtaaatg gctttaaaag gtaatccacg gcattcacct gaaaagcgga atggatatag
     7021 gaaaaatcat catatcctgt cagaaaaaca attttgcatg gatatccccg gcgtacgatc
     7081 gtctctgcca gttcaattcc actcattcct ggcatctgaa tatccgtaat cacaatatct
     7141 gccatatttt cttccaggca ctccagtgcc cttctgccat tgcgcgcggt aaatactctg
     7201 gaaatcccgt tctttttcca gtcaatataa tcccgcaatg tctccagttc aatttcttca
     7261 tcttctacaa gaagaacgcg atacattttt tccctcctgt ttccagttgc tcctgcagtt
     7321 ttcttcttct gctgcttttc atttctgtct ttttactgca gaagttcaat catatacgcc
     7381 tcatacggct gtaacttcca cacttctttt gtgagacttt ctgtatcatt atgaatcaga
     7441 accgttcctt ttgtcaaatc atatcgggct ttcatctctg ccgcggtatc ccggaaattg
     7501 cagagcacaa tcagcttttt ctctcccaga tttctctcat aagcaaaaat atccggattt
     7561 tcttcctcaa tcagacggaa atccccataa acaagagtgt catttttcct acgcagcgca
     7621 attagttttt gatagaaata gaagagggaa tccggatttt ttctgctgtt ttccacattg
     7681 atctcctcgt aattctgatt ttccgccatc cacggtgttc ccgctgtaaa tccggcgtgt
     7741 ttttccgcat tccactgcat tggcgtccgc gcatggtccc ggctgttccg tgcaagatag
     7801 gtacgaattt tttcttcctc ccagccttct tttttacggt cttcggcaaa atttacagac
     7861 tcgatatcct gatagtcctc gatccgaagc cctgacacat ttgtcatacc gatttcttcc
     7921 ccctggtaaa tataaggagt tcctttcatc ccatggagaa ggacagccaa catttttgct
     7981 gatttttccc gatacggttc cctgtcattt ccaaagcgcg atacgacacg cggaaaatca
     8041 tggtttcccc agaacaggct gttccatcct ttttcttcca gtcccgtctg ccatttttca
     8101 aagagctgct tcagttgcgg cagcgtatac ggtttcggat cccagtttcc acttcggatg
     8161 ccgtcgattc ccatcagttc aaactgaaaa atcatactga gctcctggct ttccggatca
     8221 gaatactgct gtgccatttc taccgtagca cctccggttt cccccactgt caggatctgt
     8281 tctgattttc catacgtatt ctgattcatt tccattaagt atgtatgaat ctgctctgaa
     8341 tgagtatcat ttcctccatg tccatctttt acaatattgt caattgcatc cagccggaaa
     8401 ccacggatcc ctttatccat ccagaactgg atcatccggt acagctcttt ccgaagcgac
     8461 ggatttttcc agttcagatc cggctgcatt acactgaaac tgtgataata atattcctga
     8521 acctccggca cccattccca ggtgcttcct ccaaagaatg ctccgccgcc ttctggcggc
     8581 tgctccttcg ttccttcctt ccagatgtaa aaatcatggt atttactgtt tcgatccttt
     8641 ctggcttcca ggaaccatgg gtgcagatgg gaactatgat ttaaaacaag atccatcatg
     8701 accacaattt cccgcttttc acaggctgcg atcagttctt ccagctcttc cattgtgccc
     8761 gcttcttcca ttactttata atagtcactg acatcatatc cattgtcgta attaggagaa
     8821 tcgttaaaag gcgaaagcca gatggctcca acaccaagct cctgcagata gtccagcttc
     8881 tgcagaatcc ctccaaaatc gccgatgccg tctccgttgc tgtcacagaa gcttctggtg
     8941 taaatctgat atacggttaa gctatgccac cactctcttt tcattttctg tcctctcttt
     9001 cttttttcct ataagagcag tataatgata ttatcttata ttttctaacc tgattttcgt
     9061 atttaatgaa aggattttga cttttatgaa aaaagaacgt tcaaaagagc tcttttctcc
     9121 tgaatcttgt tcccctgttc tttctggcgg aatccgggat tttaccgtgc atggaaccga
     9181 acctgttccg ctccatatct atcatcagga ttatgcgcat ggcgggctct ctgttccttt
     9241 tcactggcat aaagaattag aatggatctg ggttgaaaaa ggtactctca ctctgacact
     9301 gaaaactgaa acccggacaa tttccgaagg tacttttctt ttcatcaaca gtcacgaact
     9361 gcatcagctc tgcagcattg gaactacccc ttcggttcac catgcccttg tatttcttcc
     9421 agaaattctc tcatgtgcct atctggattc ctgtgaagca gactggattc gtccgctgct
     9481 ctctcatcaa ctgcttcttc cggcttttcc attggaagag ctttctgctg ctgaagcctc
     9541 ctctcagaca cagaaaacat ttgtactgac attatctgag cttttccagg aaatcctgta
     9601 tgcatacgat accaaaccca tcggctggtt tcttctgatc aaaagtgaca tgtatcggat
     9661 tcttgcactt ctggcgcaat accagctttt tcttcatccg gaacccctct gcactcgtaa
     9721 aaaagaaaga tctgatcagt gtaaaaaact cttacgttat attcaggata actatgattc
     9781 caaaatccgt ctttcagatc ttgctgctat actcaatatg aatccacaat atttttgtcg
     9841 tttcttcaaa acgcactttc acacaacgcc tatttcctat atcaaccagt accgcgtaac
     9901 ccaagccgca gatcttctta ccggcagctc gctttctatc ttggaaattg ctctgcatac
     9961 cggatttgaa agccccagct attttgccag cattttccgc cggacttttg gtatgtcacc
    10021 ggaagaatat cgaaaagcca tgccctgacc cggcaaattt tttccttctg aatgcacggc
    10081 aacacaaatc tcaaaaattt ttgatttttt ttcaaaaaac acttgccaaa ctcgttttct
    10141 tatgataata tactacttgt cgctgagcaa aaatcacggc agacaaatca aaaacttcgg
    10201 ctccgtggtc aagcggttaa gacatcgccc tttcacggcg gtaacacggg ttcgattccc
    10261 gtcggagtca tatggcgtcg tagccaagtg gtaaggcaat ggtctgcaac accagtatcc
    10321 accggttcaa atccggtcga cgcctct
//
LOCUS       DAOMUF010000067        10230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1601_length_10230_cov_2.874496, whole genome shotgun sequence.
ACCESSION   DAOMUF010000067 DAOMUF010000000
VERSION     DAOMUF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10230
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_10230_cov_2.874496"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLN77_05355"
     CDS             complement(<1..238)
                     /locus_tag="PLN77_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM43738.1"
                     /translation="MTITAVIAEYNPFHNGHAYQLAKARELTGADYLVVIMSGDFVQR
                     GAPAILDQHDRAELALLGGADLILQLPCHFALGSA"
     gene            454..1449
                     /gene="pta"
                     /locus_tag="PLN77_05360"
     CDS             454..1449
                     /gene="pta"
                     /locus_tag="PLN77_05360"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRM43739.1"
                     /translation="MGFIDTIKARAKADKKTIVLPESEDRRTYEAAAQILKEDLANLI
                     IIGSEEAVKKGSEGLDVSKATIVDPEKNEKTQAYVDKLVELRAKKGMTPEKARETILN
                     DYTYYGVMMVKMGDADGLVSGACHSTANTLRPCLQILKTKPGTKLVSAFFLMVVPDCE
                     YGDDGVFVFGDCGLNQNPNPEELAAIAESSAESYRMLTGNEPRVAMLSHSSKGSAKHA
                     DVDKVVEATRIAKEANPDLALDGELQLDAAIVPSVGASKAPDSKVAGKANVLIFPDLD
                     AGNIGYKLVQRLAKAEAYGPMTQGIAAPVNDLSRGCSAEDIVGVVAITAVQCQAQ"
     gene            1549..2745
                     /locus_tag="PLN77_05365"
     CDS             1549..2745
                     /locus_tag="PLN77_05365"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRM43740.1"
                     /translation="MNVLVVNCGSSSLKFQLINYDNRDVLAKGLCERIGIDGRLVYEP
                     KNGEKEVTEAAMPTHVEAIQMVLDALVNEKSGVIKSLEEVDAIGHRVLHGGMKITNSV
                     IIDDEVIKVIEECADLGPLHNPANLMGINACMKLMPGVPNVAVFDTAFHQTMPPKAYM
                     YGIPKEYYEKYAIRKYGFHGTSHKFVSKRTAEFLGKDYNDLKIIVCHMGNGASLSAVK
                     NGKCVDTSMGLTPLEGVIMGTRSGDIDPAFVEYLCNKENLTVSEVLNILNKKSGMLGM
                     TGISSDYRDVSAAAEAGNQDAINCLEAYAYRIIKYIGSYIAAMGGVDVITFTAGLGEN
                     AIPLRETICKAFEFMGLEFDSEANNCRGVEKIITKEGSKITACVIPTNEEIAICEETV
                     ELVKGK"
     gene            2820..3347
                     /locus_tag="PLN77_05370"
     CDS             2820..3347
                     /locus_tag="PLN77_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRM43741.1"
                     /translation="MKISLTKLFAQDGKSEKYEIPLTSETFSIGGQDYRVIEKHPVIL
                     GLTHEGNRVITITGSADLTMEIPCARCLTPVATPFQLEFDLQIDLNLSEEEREEKEED
                     SSFVEGDELDVEELVRNELIVQWPIRVLCKDDCKGICSRCGANLNIQTCDCDTTGLDP
                     RMAAIKDIFSKFKEV"
     gene            3350..3529
                     /gene="rpmF"
                     /locus_tag="PLN77_05375"
     CDS             3350..3529
                     /gene="rpmF"
                     /locus_tag="PLN77_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRM43742.1"
                     /translation="MSICPKNKSSKARRDKRRANWKMSAPNLVKCSKCGALMMPHRVC
                     KACGSYNKKEVVAMD"
     gene            3737..4309
                     /locus_tag="PLN77_05380"
     CDS             3737..4309
                     /locus_tag="PLN77_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43743.1"
                     /translation="MKNRRIIFLAACMCSVVFLSGCSTDSLMDKMMGTETTVSSSASV
                     DISKEDSDAVHVDANLEKPVFSVNPVESLQIPVGNTSGKLTCEAGITGEGTVTYQWYK
                     NNVNSNGGGTPIEGATEVTYQVDTSAEGKDYYYVVATNTVGNTVSMATSTVTEVTVIP
                     AGKWVQNENGWQYQNNDGSYAEYRRLLVRV"
     gene            4263..4508
                     /locus_tag="PLN77_05385"
     CDS             4263..4508
                     /locus_tag="PLN77_05385"
                     /inference="COORDINATES: protein motif:HMM:NF040203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43744.1"
                     /translation="MMEAMQNIDGYWYVFDENSYMVTGWYWSGEEWYYLADNGQMQTG
                     WFTQDGEEYYLDPDTGVMARNTTIDGHEMNSSGVKVS"
     gene            4737..>4835
                     /locus_tag="PLN77_05390"
     CDS             4737..>4835
                     /locus_tag="PLN77_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid/phospholipid biosynthesis enzyme"
                     /protein_id="HRM43745.1"
                     /translation="MSDLVRVAVDAMGGDYAPVEPVKGAVEAVNADE"
     assembly_gap    4836..4845
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4846..5770
                     /gene="plsX"
                     /locus_tag="PLN77_05395"
     CDS             <4846..5770
                     /gene="plsX"
                     /locus_tag="PLN77_05395"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006906092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRM43746.1"
                     /translation="VGQVDVIEKELQKYTYPKERVEIVPATEIIETGEPPVNAIRHKK
                     DSSIVVGMKKVRYGEADAFVSSGSTGAVLVGGQTIVGRIKGVERPPLAPLIPTKDGVS
                     LLIDCGANVDARPSHLVQFAMMGSIYMEHVVGIKNPRVGIVNIGVEEEKGNALVKETY
                     PLLKENPYINFVGSIEAREIPNGAADVVVCEAFVGNVILKLYEGVGATLISKVKQGMM
                     TSLRSKIGALLVKPALKETLKSFDASEYGGAPLLGLKGLVVKTHGSSKANEFKNSILQ
                     CISFSEEKIPQKVQEHLADYKSSREAAKDGV"
     gene            5760..5993
                     /gene="acpP"
                     /locus_tag="PLN77_05400"
     CDS             5760..5993
                     /gene="acpP"
                     /locus_tag="PLN77_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRM43747.1"
                     /translation="MEFEKLQEIIADVLNVDPNEVTMETTFADDLGADSLDVFQIVMG
                     IEEEFDIQIEAEEAEHIVSVEDAVEAIKKKLAE"
     gene            6099..6791
                     /gene="rnc"
                     /locus_tag="PLN77_05405"
     CDS             6099..6791
                     /gene="rnc"
                     /locus_tag="PLN77_05405"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRM43748.1"
                     /translation="MNNIDKLVDLEKKIQYKFQDFGLLEMAMRHSSYANEHHMERLEC
                     NERLEFLGDAVLEVVSSEFLYKTFPRLPEGEMTKKRASLVCEPALAFCAREIDLGSFL
                     LLGKGEEATGGRHRDSIVSDAMEATIGAIFLDGGFEPAKTFVLNYILNDIEHKQLFYD
                     SKTILQELIQGETDEEIVYTILKEEGPDHDKSFWAQVSVGKKALGTGKGRTKKAAEQA
                     AAYDAILARKKA"
     gene            6799..>10230
                     /gene="smc"
                     /locus_tag="PLN77_05410"
     CDS             6799..>10230
                     /gene="smc"
                     /locus_tag="PLN77_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRM43749.1"
                     /translation="MYLKSIEMQGFKSFANKTILQFHDGITGVVGPNGSGKSNVGDAV
                     RWVLGEQSAKQLRGGNMQDVIFSGTETRKPLGYASVAITLDNSDHKLNVDFEEVTVTR
                     RLYRSGESEYLMNGASCRLKDINELFYDTGIGKEGYSIIGQGQIDKILSGKPEERREL
                     FDEAAGIVKFKRRKAVTEKKLAEEQQNLVRVNDILSELTRQLEPLERQSETAKIYLKK
                     KEELKILDINMFLLEMERIRNDLAKVEDRYRIAEEQLKSTKEAFEHTKQEYEKLEQEQ
                     EKLDQTILSARDQAGENALKRQQLENQIEVLNEQIHASERNSDTLRERLEAIRAESRT
                     KTAEKNTYQAEKDGLHEALLASETEKSEKEAALSECQGQIQAVELQSEQDKSEVIGLL
                     NQRGSTKGKMQRYDAMLEQIGIRKAELSQRVLHLKTSESEQNGQMEELQNAFEALEET
                     VSSLKQQYQEADQKITALTADLSNQTKEMEAGQTNYHREASRLESLKNITERYDGYGN
                     SIRKVMEQKDRNPGIHGVVADLLQAEKKYETAIETALGGSIQNIVTDNEATAKYLIEY
                     LKRGHFGRATFLPLTSMRGKKAQVNEDAARETGVLGVANALVSYDPVYEGLVAQLLGR
                     TLVVDTIDHAIVIQRKYRQTLRMVTLEGELLSPGGSMTGGSFRNNSNLLGRRREIEEL
                     EKSVQGLAKELREIQQELEEKKNKRNEIRDLRTELNEKLQRQYIEQNTVRLNLEKARE
                     KKTEIRLDFEALQKESLEIENQIAEIERNRKVIGQEMQESVERESTLQENIQKAQEAL
                     TGLRRREAEYTKDLEEIRLNYANLSQKDGFLAQNIRRLEEETGKLEAESRTMLEKAAE
                     EKDDIKKKQQDIVEIQKTIAAAVQAKEEEDRRIVELQEKKEALSADHKEFFEKRDQLS
                     DQISLMDKECFRLNSQKEKLEETKEAQTSYLWEEYEVTPGHAREFQMEEIPERSELKR
                     RVLEVKDAIRKLGSVNVNAIEEYKEISERHAFLDAQHTDLVKAAETLQGIIDELDAGM
                     RKQFTEKFEEIRKEFDRVFRQLFGGGKGTLELEEDTDVLEAGIRIISQPPGKKLQNMM
                     QLSGGEKALTAIALLFAIQNLKPSPFCLLDEIEAALDDSNVTRFAQYLHKLTKNTQ"
BASE COUNT         3083 a         2184 c         2860 g         2093 t
ORIGIN      
        1 gtgcactgcc gagtgcaaag tgacacggaa gctgtaagat aaggtccgcg ccgccaagaa
       61 gcgccagctc cgcacggtcg tgttggtcaa gaatcgcagg tgcgccgcgc tggacgaaat
      121 cgccgctcat aatcacgaca agatagtccg ccccggtcag ttccctggct tttgccagct
      181 gataagcatg gccgttatga aaaggattat attctgcaat gactgcggta atggtcataa
      241 aacgtacctt ctttcgaaaa aaatagcacg attccaaaca gagaggaacc gcggatcaca
      301 tagccctatc gtaacaaaaa cagaaaagga atgcaagaat tcctccgttt tggatttttt
      361 gtccagttgt tttgaatttt atacttgcga aagggcaaaa agcgtactac aatgagaata
      421 tcgagtaaat ttatcgaaag gagtcaaaag atcatgggat ttattgacac aattaaagca
      481 agagcaaaag cagacaaaaa aacaattgta ctgccagagt cagaagacag aagaacatat
      541 gaggctgctg cacagatcct gaaagaggac cttgcaaatc tgatcatcat cggaagcgaa
      601 gaggcagtaa agaaaggcag cgagggactg gatgtctcca aagctactat tgtagatcca
      661 gagaaaaacg agaaaacaca ggcttatgta gataagctgg tagagcttcg tgcaaagaaa
      721 ggcatgactc cggagaaagc aagagagacc atcctgaatg actatacata ttatggcgtt
      781 atgatggtta aaatgggaga tgcagacgga cttgtttccg gtgcctgcca ttctaccgca
      841 aacaccttac gtccatgcct gcagatctta aagaccaaac caggcacaaa actcgtatcc
      901 gcattcttcc tgatggtagt tccggactgc gagtacggcg atgacggtgt attcgtattc
      961 ggtgactgtg gtctgaacca gaacccgaat ccggaagagc tggctgcaat cgcagagtct
     1021 tccgcagagt cctaccgcat gctgaccgga aacgagccaa gagttgctat gctttcccac
     1081 tcctccaaag gaagtgcaaa acatgctgat gtagacaaag ttgttgaggc taccagaatt
     1141 gcaaaagaag caaacccgga tctcgctctc gacggcgagc tgcagcttga tgccgctatc
     1201 gttccaagcg taggtgcatc caaagctccg gacagcaaag tagcaggaaa agcaaacgtt
     1261 ctgatcttcc cggatctgga tgccggaaac atcggataca aactggtaca gagactggca
     1321 aaagcagaag catacggccc gatgacgcag ggtatcgcag ctccagtcaa cgatctgtcc
     1381 cgtggatgct ccgcagagga tatcgttggt gttgtagcaa tcactgctgt acagtgccag
     1441 gcacagtaag cacgaaataa aaccaattac aagcggtttt ctggccggct gcggagaaca
     1501 ggccggccag aagttttaat aagaatttta aaataggaga atttagaaat gaacgtatta
     1561 gtagtaaact gcggaagttc ctctctgaaa ttccagctga tcaattacga taaccgtgac
     1621 gtcctggcaa aaggactctg tgagagaatc ggtatcgacg gacgcctcgt ttatgagcca
     1681 aagaacggcg aaaaagaggt aacagaagca gcaatgccga cacacgtaga ggcaatccag
     1741 atggttctgg acgctctggt aaacgagaag agcggcgtca tcaagagcct ggaagaagta
     1801 gatgccatcg gacaccgcgt tctgcacggc ggaatgaaga tcacaaactc tgttatcatc
     1861 gatgacgaag taatcaaagt tatcgaggag tgcgctgacc ttggtcctct gcacaatccg
     1921 gcaaacctga tgggaatcaa tgcctgcatg aaactgatgc cgggcgttcc gaacgtagca
     1981 gtatttgata ccgcattcca tcagaccatg cctccgaaag catatatgta cggtattccg
     2041 aaagagtact atgagaaata cgcaatccgt aaatacggct tccacggaac ttctcataaa
     2101 ttcgtatcca agagaaccgc agagttcctt ggcaaagact acaacgatct gaagatcatc
     2161 gtatgccata tgggaaatgg tgcttccctt tccgctgtaa agaacggcaa atgtgtagat
     2221 acctccatgg gtcttacccc gctcgaaggt gtgatcatgg gaacccgttc cggagatatc
     2281 gatccggctt ttgttgagta tctgtgcaac aaagagaacc tgaccgtatc cgaggttctg
     2341 aacattctga acaagaaatc cggtatgctc ggcatgaccg gcatctccag cgattaccgt
     2401 gatgtatctg cagcagcaga ggcaggcaat caggatgcca tcaactgtct ggaagcatac
     2461 gcttaccgca ttatcaaata catcggttcc tacatcgcag ctatgggcgg cgtagatgtg
     2521 atcaccttca ccgcaggcct tggagagaac gctattccgc ttcgtgagac catctgcaaa
     2581 gcatttgagt tcatgggtct ggaattcgat tctgaggcaa ataactgccg cggcgttgag
     2641 aagatcatca ccaaagaagg ctccaagatt acagcatgcg tgatcccgac caacgaggaa
     2701 atcgcaatct gcgaggagac cgtagagtta gtaaaaggaa aataaaaaat aattcaaaaa
     2761 gcagttgaca attcttacct tcccgggtat aattggtaaa gtgtgattag gagaacaata
     2821 tgaaaatcag tctgaccaag ttatttgcgc aggacggaaa gtccgaaaag tatgaaattc
     2881 cgcttacatc cgaaactttt tcgatcggcg ggcaggacta tcgggtaatc gaaaaacatc
     2941 ctgttatcct tggccttacg catgaaggca atcgtgtgat cacgatcacc ggcagtgcag
     3001 accttacaat ggagattcca tgtgcaagat gcctgacacc ggtagcgact ccattccagc
     3061 tcgagttcga tctgcagatt gatctgaatc tgtcagagga agaacgcgag gaaaaagaag
     3121 aggactcttc gtttgtggag ggagacgagc tcgatgtgga agaactggtt cgtaatgagc
     3181 tcatagtcca atggccgatt cgagtgctgt gtaaagatga ctgtaaagga atctgcagcc
     3241 gttgtggtgc gaatctgaat attcagacct gtgattgtga taccacaggg ctagaccccc
     3301 gaatggctgc aatcaaagat atattcagca aatttaagga ggtgtaacta tgtccatttg
     3361 tcctaagaat aaatcttcca aagcaagacg cgataagaga agagcaaact ggaaaatgtc
     3421 tgctccgaac cttgtaaaat gcagcaaatg tggtgctctt atgatgccgc acagagtttg
     3481 caaagcttgt ggttcttaca acaaaaaaga agtcgttgca atggactaat attcataaat
     3541 cggaatcagt tccgatgtgc aatgcagcaa aaggctcccg tcaccggggg cttttttgtt
     3601 gtctctgcgg gttgtctgga agcgtcatat aaaagatttg acgtggtgtg tcgtgatgtg
     3661 ctataatggt ctcggtgaga attgaaacaa tattgcaaca ttttcgtaac aattataaaa
     3721 aaggagtgca aagcatatga agaatagacg aatcatattt ctggcagcat gcatgtgcag
     3781 tgtagtattc ctctcaggat gttccacaga cagtctgatg gataaaatga tgggaactga
     3841 gacgacggtt tcgtcttcgg ccagtgtaga tatttcaaaa gaggacagcg atgctgttca
     3901 tgtagatgcc aatctggaaa agccggtttt ttcggtaaat ccggtggaga gcctgcagat
     3961 tcccgttgga aatacatctg gaaagctgac ttgtgaggca ggaataacgg gagaaggcac
     4021 agtaacatat cagtggtata agaacaacgt aaattccaac ggcggaggaa cgccgattga
     4081 aggcgcaaca gaagtgacat accaggtcga tacttcggcg gagggcaaag attactatta
     4141 cgttgtggca acaaacacgg ttggaaatac cgtttccatg gcgacgagta cggtaacaga
     4201 agttacggta attccggcag ggaaatgggt tcagaatgaa aacggatggc agtatcagaa
     4261 taatgatgga agctatgcag aatatcgacg gctactggta cgtgtttgat gaaaattcat
     4321 acatggtgac aggatggtac tggagcggag aagaatggta ttatctggcg gacaacggtc
     4381 agatgcagac cggctggttc acgcaggatg gtgaggaata ttatctggat ccggataccg
     4441 gtgttatggc acggaataca accatagacg gtcatgagat gaattcgagc ggagtcaagg
     4501 tttcttaaga aaaataagac aaaacagagc gggtggattt tggcaagata aatccacccg
     4561 cttttttgga atacaggaga aagcgcggat gggcatttgt gcttgatttt tgcaaaggag
     4621 tttagtatag ttaatacagc gcttgcataa gtgcttttag agcgtggttg aaaacggttt
     4681 tttgaaagaa aaagagtttt caaacgcgct ttagaaaaga gaaaagagga aaaaacatga
     4741 gtgacttagt cagagttgca gtggacgcga tgggcggcga ttatgcgcct gtggagccgg
     4801 taaaaggcgc cgttgaggcc gtaaacgcag atgaannnnn nnnnnggtcg gccaggtgga
     4861 tgtcatcgaa aaagaactgc agaaatatac gtacccgaag gagcgggttg agatcgttcc
     4921 ggcaaccgag atcattgaga cgggagagcc gccggtcaat gcgatccgcc acaaaaaaga
     4981 ttcttcgatc gttgtcggca tgaaaaaagt gcgctacgga gaggcagacg cgtttgtctc
     5041 atccggaagc accggagcag tcctggtcgg cggacagacg atcgtaggac gcatcaaggg
     5101 cgtagagcgc ccgccgctgg ctccgctgat cccgacgaaa gacggtgtat cccttctgat
     5161 cgactgcggt gcgaatgttg atgcaagacc gtcccatctg gtgcagtttg ccatgatggg
     5221 ttccatctat atggaacacg ttgtcggcat taaaaatccg cgtgtgggaa tcgtgaatat
     5281 cggtgtggaa gaagaaaaag gaaacgcgct cgtcaaagag acgtatccgc ttctgaaaga
     5341 aaacccgtat atcaactttg tcggcagtat cgaagcaaga gaaattccaa acggagccgc
     5401 agacgttgtt gtctgtgagg cgtttgtcgg aaacgttatc ttaaagctgt atgagggcgt
     5461 cggcgcgacc ctgatttcca aggtaaaaca gggcatgatg acatctctgc gcagtaaaat
     5521 cggagccctt cttgtgaaac cggcgctgaa agagacactg aagtcttttg atgccagtga
     5581 gtacggcgga gcaccgctgc ttggcttaaa aggactcgtt gtaaaaacgc acggaagctc
     5641 aaaagccaac gaatttaaaa attccattct ccagtgcatt tcattcagcg aggagaagat
     5701 tccgcagaaa gtgcaggagc atctggcaga ctataagagc agcagggagg cagcgaaaga
     5761 tggagtttga aaaattacag gagatcattg cggatgtatt aaacgtagat ccgaatgaag
     5821 taaccatgga gacaaccttt gcagatgatc tgggagcgga ttccctggat gtatttcaga
     5881 ttgtgatggg aattgaagaa gaatttgata tccagatcga agcagaagaa gcagagcaca
     5941 tcgtatcggt tgaggatgcg gtggaagcga tcaaaaagaa actggcagaa taatagaaaa
     6001 tggcgaaggg gctgtttgct acaggacagc ccctttgtta ccgtccgcag aacacctgca
     6061 aacggtaact atgaggaagg acaggcttaa aacaggttat gaataatatt gataaactcg
     6121 tggatttgga aaagaagatc cagtataaat ttcaggattt cggactgctg gagatggcga
     6181 tgcgccattc ttcctacgca aatgagcacc atatggagcg cctggagtgc aacgagcgtc
     6241 tggagttctt aggagatgcc gtgctggaag ttgtatccag cgaattttta tacaagacat
     6301 tcccgcgtct cccggagggc gagatgacga aaaaacgcgc cagcctcgtc tgtgaaccgg
     6361 cactggcatt ctgtgcccgt gagattgacc ttggcagctt tctgctcctt ggaaagggcg
     6421 aggaggcaac cggcggccgt caccgcgatt ccatcgtttc ggatgccatg gaggcaacga
     6481 tcggtgcgat tttcctggac ggcggctttg aaccggcaaa aacgttcgtt ctgaactata
     6541 tcttaaacga tatcgaacac aaacagcttt tctacgacag caaaacgatt ctgcaggagc
     6601 tgatccaggg tgagacggat gaagagattg tttacaccat cttaaaagaa gagggaccgg
     6661 atcacgacaa gagcttctgg gcacaggtca gcgtcggaaa gaaagcgctc ggtaccggaa
     6721 agggcagaac gaaaaaagcg gcagaacagg cagcggcata cgatgcaatt cttgccagaa
     6781 aaaaagcgta ggtgaattat gtatctcaaa tccattgaaa tgcagggatt taagtcgttt
     6841 gccaacaaaa ccattctgca gttccacgac ggtattaccg gcgttgtagg gccgaacggc
     6901 agtggaaaga gcaatgttgg cgatgcggtg cgctgggtgc tcggcgaaca gagcgccaag
     6961 cagctccgcg gcggaaatat gcaggatgtt attttctccg gaaccgagac gagaaagccg
     7021 ctcggctacg cgtcggttgc aattacactt gacaattccg accacaagct gaatgtcgat
     7081 ttcgaggaag tcacggtgac gagacggctc taccgatccg gtgagagtga gtatctgatg
     7141 aacggcgcat cgtgccggtt aaaggacatc aacgagctgt tctacgatac cggtatcgga
     7201 aaagaaggct attccatcat cggacagggt cagattgata agatcttaag cggtaagccg
     7261 gaagagcgcc gggagctgtt tgacgaggcg gcaggaattg tcaaattcaa gagaaggaag
     7321 gctgtgaccg aaaagaagct ggcggaagag cagcagaatc tggtgcgcgt caacgacatt
     7381 ctctcggaac tgacaaggca gttagagccg ctggaacgcc agtctgagac ggcaaagatt
     7441 tatttaaaga agaaagagga attaaagatc ctcgatatca atatgtttct gctcgagatg
     7501 gagcgcatcc ggaacgatct cgcgaaagta gaagaccggt accggattgc agaagagcag
     7561 ttaaagagca cgaaagaggc ttttgagcat acgaagcagg agtatgaaaa gctggaacag
     7621 gagcaggaaa aactggacca gacgattctt tctgcgcgcg atcaggcggg ggagaatgca
     7681 ctgaaacgcc agcagctcga aaaccagatc gaggtgttga acgagcagat tcatgcatcg
     7741 gagaggaaca gcgacacatt aagagaacgt ctggaggcga tccgcgcgga gagccgcaca
     7801 aagacggcag aaaaaaatac atatcaggca gaaaaggacg ggcttcatga agcacttctt
     7861 gcgtcagaga cggaaaagag tgagaaagag gccgctctct cggaatgcca ggggcagatc
     7921 caggcagtcg agctgcagag tgaacaggat aagagcgaag tcatcggcct tctgaaccag
     7981 cggggttcca cgaagggaaa aatgcagcgc tacgatgcga tgctcgagca gatcggcatc
     8041 cgaaaggcag aattaagcca gagggtgctg catttaaaga cgagcgagtc cgaacagaac
     8101 gggcagatgg aagaactgca gaacgcgttt gaagcgctcg aagagacggt aagcagcctg
     8161 aaacagcagt accaggaggc agatcagaag attaccgcgc tgacggcgga tctctcgaac
     8221 cagacgaaag agatggaagc cggccagacc aattaccaca gagaagcgtc aaggctcgaa
     8281 tccttaaaga acatcaccga gcggtacgac ggctatggaa acagtattcg gaaggtcatg
     8341 gagcagaagg accggaatcc cggcatccac ggcgttgtgg cggatcttct gcaggcggag
     8401 aaaaagtacg agacggcgat cgagaccgca cttggcggca gcattcagaa tattgtcacc
     8461 gacaatgagg cgacggcgaa atacctgatt gaatacctga aacggggcca tttcggccgt
     8521 gcgacgttcc ttccgctcac cagtatgcgc gggaaaaaag cgcaggtgaa tgaggatgcg
     8581 gcaagagaaa ccggtgtgct cggtgtggca aatgcacttg tttcgtacga cccggtgtat
     8641 gagggccttg tggcacagct gcttggaaga acgctggtgg tcgacacgat cgaccatgcc
     8701 attgtgatcc agcggaaata ccgtcagacg ctccgcatgg taaccctcga gggcgagctc
     8761 ttgagcccgg gcggttccat gacaggaggt tcgttccgga acaacagcaa cctgctcgga
     8821 agacggcgtg agattgagga actcgaaaag agcgtacagg gtcttgcgaa ggaactgcgg
     8881 gaaatccagc aggaacttga agagaagaaa aataagcgga atgaaatccg cgatctccgc
     8941 accgagctga atgaaaaact gcagcggcag tacatcgagc agaatacggt gcgcctgaat
     9001 ctggaaaaag cgcgcgagaa aaagacggaa atccgtctgg attttgaagc gcttcagaag
     9061 gagagtctcg agattgaaaa tcagatcgcc gagattgagc ggaaccggaa agtcatcggt
     9121 caggaaatgc aggaatcggt ggagcgggaa agcacgctgc aggagaatat ccagaaggcg
     9181 caggaggcgc ttacagggct tcgccgccgg gaagcagaat atacgaagga tcttgaagaa
     9241 atccggttaa attacgcaaa tctgtcccag aaggatggat tccttgccca gaacatccgc
     9301 cgtcttgagg aagagacggg aaaactggaa gcggaaagcc gcaccatgct ggaaaaagcg
     9361 gcggaggaaa aagacgatat caaaaagaaa cagcaggaca tcgtggagat ccagaagacg
     9421 attgcagcgg cagtgcaggc aaaagaagag gaagaccgcc ggattgtgga actgcaggag
     9481 aaaaaagaag cattaagcgc agatcacaaa gaatttttcg agaagcggga tcagctctcc
     9541 gaccagattt ccctgatgga caaagagtgc ttccgtctga acagccagaa agaaaagctg
     9601 gaggagacaa aagaggcaca gacctcgtat ctctgggaag agtacgaagt gacaccgggc
     9661 cacgcaagag agttccagat ggaggagatt ccggagcgca gtgaattaaa gcggcgtgtg
     9721 ctggaagtaa aagatgcgat ccggaaactt ggctctgtca atgtgaatgc gattgaggag
     9781 tacaaagaga tttcggagcg ccacgcattt cttgatgcac agcacactga tcttgtcaaa
     9841 gcggcggaga cactgcaggg catcatcgat gagctggatg caggtatgcg taagcagttc
     9901 acggaaaaat tcgaggagat ccggaaagag tttgaccgcg tgttccgcca gctgttcggc
     9961 ggcggaaagg gaacgctgga gctcgaggag gacacggatg tactcgaggc aggtatccgc
    10021 atcatttccc agccgccggg aaagaaactg cagaatatga tgcagctctc cggaggcgag
    10081 aaggcactga ccgcgattgc gctgcttttt gcaatccaga acttaaagcc gtccccgttc
    10141 tgtttacttg acgagatcga ggcggcgctc gacgattcca acgtaacgcg ttttgcgcag
    10201 tatctgcaca agctgacaaa aaatacccag
//
LOCUS       DAOMUF010000068         9827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1701_length_9827_cov_2.625153, whole genome shotgun sequence.
ACCESSION   DAOMUF010000068 DAOMUF010000000
VERSION     DAOMUF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1701_length_9827_cov_2.625153"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1050
                     /gene="larB"
                     /locus_tag="PLN77_05415"
     CDS             298..1050
                     /gene="larB"
                     /locus_tag="PLN77_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRM43750.1"
                     /translation="MEAREVLEKVKNNEISIEEAEKYFKKEPFEEMGYAKLDTHREIR
                     SGFAEVIFCARKADEHLLKIFTRLYEQDGEVFGTRASEHQYELIREKFPEVVYDPLSH
                     ILKIEKKDKKRIGKIAVCTAGTADISVAEEAAQTAEYFGANVERIYDVGVSGIHRLLS
                     RLDTIQSANCVVAVAGMEGALASVLGGLVDKPVIAVPTSVGYGASMNGLSALLTMINS
                     CANGIAVVNIDNGYGAGYIATQINRMGVHQDE"
     gene            1043..2344
                     /gene="larC"
                     /locus_tag="PLN77_05420"
     CDS             1043..2344
                     /gene="larC"
                     /locus_tag="PLN77_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HRM43751.1"
                     /translation="MSKSLYLECGSGISGDMTVAALLDLGADREVLEKSLESLKLPGY
                     EIAITRVKKSGLDACDFNVILDAEHENHDHDMAYLHGDGAEVHDHPHDHEHHHDHDHT
                     GEHHHHHEHRGLPEILAIIRSGGLTPGARALAERIFQILAEAEAKAHGVPLDQVHFHE
                     VGAVDSIVDIVSAAVCLDNLAPDEVIVTGLSEGSGFVRCQHGMIPVPVPAVLNIVQAH
                     GLTLVPTGIQGELVTPTGAAIVAAIRTKETLPASFKCTRTGLGAGKRTYERPSLLRAM
                     MLETEENDEKDTIWKLECNIDDCTGEALGYCMGKLLRSGARDVHYIPVYMKKNRPAYQ
                     LDVICDDTTRETLENIIFAETTTIGIRRCRMERTVMKREFATIATEYGDATVKICRHG
                     EIEKIYPEYESAAVIAEKSGMPVGEAGAWIVQKYKEGNKKA"
     gene            2341..3516
                     /gene="fliB"
                     /locus_tag="PLN77_05425"
     CDS             2341..3516
                     /gene="fliB"
                     /locus_tag="PLN77_05425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HRM43752.1"
                     /translation="MRYLKPHFYDQFVCTAGDCPDTCCAGWQIVIDEDSLERYGNEKS
                     EFGKRLRNSIDWEEECFYQNNRRCAFLNDENLCDLYKELGPDSLCDTCRLYPRHTEEY
                     EGLRELSLSLSCPEAARIILSCKEPVRFLEEETDEEDDFEEFDFMMFSQLEDTRDVLF
                     RILQNRELPLQERMTAAEQLAEQYQICMEEQREYDIDDLLRKYEKHLEEGTLSECVAE
                     SLAEKGVDAASFHAYDRQVKELAVLRGLERLRPEWDTVLDGTEKALYQNGETAYQAIC
                     EEFHRTWGAAGERRAEWENIGEQLLMFFVYTYFCGAVYDDMVCSKMELALFSVRWIQE
                     ILVARWLENGKTLSMHDIEELSWRYAREVEHSDDNLNALEDWLFETYAPEGCVLEEE"
     gene            3602..4249
                     /locus_tag="PLN77_05430"
     CDS             3602..4249
                     /locus_tag="PLN77_05430"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HRM43753.1"
                     /translation="MQEKQKKWILRIALYLIGIMVLALGITLNTKTNLGVSPIISMPY
                     AVAQIFGYETGTAVFVAYLIFIVLQIVLLKGKFPPFQLLQVAASYLTSAFITIFDRIL
                     PAPDQMGMRLLVLALAIILTGIGAAVTVDVNIVPNPADGLARVLGERCGKNMGFGKNL
                     FDFSAIVISLAIGLIGAGRPVGIGIGTVIAMVCTGRVIAAFNHFAKKKIEEITIA"
     gene            4387..4872
                     /locus_tag="PLN77_05435"
     CDS             4387..4872
                     /locus_tag="PLN77_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRM43754.1"
                     /translation="MNLVITMSRRFGTGASIIAKELSERLHIPVYDKDDVEHGMRENA
                     FESEADAIRELAKQPCIIIGRCASEFLKDKSNVINIYVCADKEDRIKRIMKLFSLTRE
                     AAEVMLEETDKQRAEYYYKNTGKTWGDVNNYHMILNTSDLGIENCADILMRYFEMKDY
                     I"
     gene            complement(4947..>5476)
                     /locus_tag="PLN77_05440"
     CDS             complement(4947..>5476)
                     /locus_tag="PLN77_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HRM43755.1"
                     /translation="GIVLLVIGLAFILIRIFVLAPFEVGGSRFFIENSMEKAGLGNIL
                     FGFQNGYYGKTVWTLFLKNLYIFLWSLLLLIPGIVKAYEYHMVPYLLADYPELSTEEA
                     FRISREMMSGEKMNTFILDLSFIGWYILSGITCNLVGIFYLYPYKYATDAELFLVLKQ
                     NYFSSRSYGNTYQAY"
     assembly_gap    5477..5508
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5509..5758)
                     /locus_tag="PLN77_05445"
     CDS             complement(<5509..5758)
                     /locus_tag="PLN77_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43756.1"
                     /translation="MWTREELKTRGKAAFLRNYWICVVAALILTLLVGSGTSNSGNSN
                     NDSHQTNSVGISGFTISSDNFNVESFAERFTPLGPVKFV"
     gene            6055..6966
                     /locus_tag="PLN77_05450"
     CDS             6055..6966
                     /locus_tag="PLN77_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HRM43757.1"
                     /translation="MYLTKGEFLQKFGILPQEFEEADISWEELLEIADDYERRRPTLE
                     KIRKEFVAEFLQDKEKEIGLQSYHSRLKDTEHLVEKLVRKRLENYAKYRKMDATNYMR
                     YVTDLIGIRGLLLYREDWVNFHKYIIHWFKNDPEKYIRDYGRDYDQNASGYMAEPPKV
                     HTRLGDYADIYVNWIPEENILDRKHYRAVHYIVVYRGVYIEIQIKTLFEEGWGEIDHS
                     ILYPRRKGNAMLTEFSELLNRLAGMGDEMGSFYRRLQVVPDEKFQSKETIVRKRELKP
                     QVKAAVEKRDLNEIHTMDDAVWSILKE"
     gene            6999..7226
                     /locus_tag="PLN77_05455"
     CDS             6999..7226
                     /locus_tag="PLN77_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43758.1"
                     /translation="MDGRNTGRMGFSHAAEDSAENMMAAVKAGKIRKHLMGNTSPIRY
                     GTMEKREMTEYTDFVRTKLCYEILNLPGEEK"
     gene            7223..7777
                     /locus_tag="PLN77_05460"
     CDS             7223..7777
                     /locus_tag="PLN77_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43759.1"
                     /translation="MNTIEKIYTNYDGLLEEFSEEVIQSRYAVFYEEIEEFAKSLGIR
                     EKIQISESLLSHAVLDYFTDISRLKHFHQAKHINSLKVISYETYWLLRRKPIQILVED
                     ETSDAMAFLNEKFVFSRIAKYLMGDGKRVILSPETKKGFLNYLDSLFYYLKYRNYDAE
                     MLEMMLMGFKAGVLVADDLKEQES"
     gene            7980..9404
                     /locus_tag="PLN77_05465"
     CDS             7980..9404
                     /locus_tag="PLN77_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HRM43760.1"
                     /translation="MATLEKINAFLKAVNAAAWGPPLLFLLLGTGIFLTIQLRALPIR
                     RLFFALRTAFGREARYSGEKTEAGVSPFASLMTELAAEIGTGNIVGVASAMALGGPGA
                     LGWMMLAAVVGLALKFSESFLSVQYRMQRKDGTYCGGPMVTLTRAFPGERLAKMLAVF
                     FAGSAVLVSLGMGNMTQANSIALAMQDTFGVAREKSALVLTILLLLVVTGGLRSIAKA
                     AEVLVPGMALFYLGGAGMVIGLHAGELPRTLLAIVRDAFSFRAASGGAVGAGISLLPP
                     ALRYGISRGVFSNEAGLGAGGISAAAAQTDDPVRQGYISMTGVFVDTMVICLVTGLAV
                     GVTGAAEGVLEAEKADGAAMVIQAFESVFGPAGGYVVAVGILLFAFATMAGWAYQGEQ
                     AFLWLVKKDSFGMVYRVFFCFAAFAGCVCMAETVWNFAELANACMAVPNLLCVLRLWK
                     EVKEEAFRFESRIKKAEQKKDRNT"
     gene            complement(9424..>9827)
                     /locus_tag="PLN77_05470"
     CDS             complement(9424..>9827)
                     /locus_tag="PLN77_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM43761.1"
                     /translation="WSHPDMSKISTQEAFSEELNRVSTLYQETTGQEMVKFYRPPQGK
                     YSTQNLQMAKDLGYHTFFWSLAYVDWYQDQQPTHEEAFKKLTGRIHPGAIVLLHNTSS
                     TNAEILDELLTKWEEMGYSFGSLDQLASKSA"
BASE COUNT         2621 a         2112 c         2817 g         2245 t
ORIGIN      
        1 gttttccaca tacgattgac ggtgtcgatg aacatgaata ttttgagtca attccatacg
       61 cgggatgact cacaccgcca aaagaaggga ttgcgggaac gcagaaagca gggtatactt
      121 gtgtatagaa tctgcttgac ttaaagtgca cttcatagtt taaaataaag aaagctggag
      181 atttgccgtg tcagaaaaga gaaggttctg gcagagcaga ggtttcagct ttctttttat
      241 gttgcatcgt atgtgggtgt gaaagaaaag ggaaaaaaga aaaacgaggt gaatgttttg
      301 gaggcaagag aagtattaga aaaagtaaaa aacaacgaaa tctcgatcga ggaggcagag
      361 aaatatttca aaaaagagcc gttcgaggag atgggatatg cgaagctgga tacgcaccgt
      421 gaaatccgga gcggatttgc ggaggtcatt ttctgtgcgc ggaaggcgga tgaacatctg
      481 ctgaagattt ttacgcgtct gtacgagcag gatggggagg ttttcggaac gagagccagt
      541 gagcatcagt atgagctgat ccgcgaaaag tttccggaag tggtgtatga tccgctgtcg
      601 catattttaa aaatagaaaa gaaagacaag aagcggatcg gaaaaattgc ggtctgtacg
      661 gctggaactg cggatatttc cgttgccgag gaggcggcgc agaccgcaga atacttcggt
      721 gcaaacgttg agcgtatcta tgatgtcggt gtgagcggta tccaccgcct gctgtcgcgc
      781 ctcgatacga ttcagagcgc caactgcgtt gtagccgttg ccggaatgga gggggcgctg
      841 gcgagcgtgc tcggcggctt ggtggacaag ccggtgattg ctgttccgac atccgttggc
      901 tacggggcca gcatgaacgg tctttcggcg ctgcttacga tgatcaattc ctgcgcgaac
      961 ggaattgccg tcgtgaatat cgacaacggc tatggagccg gatatattgc aacacagatt
     1021 aacagaatgg gagtacatca agatgagtaa aagtttatat ctggagtgcg ggagcggaat
     1081 cagcggagat atgaccgttg cagcgctgct cgacctggga gcagaccggg aagtgctgga
     1141 gaaatcactg gaaagcctga aactgccggg atacgaaatt gcgattacaa gagtgaaaaa
     1201 gtcggggctg gatgcctgcg atttcaatgt gattctggat gcagagcatg agaaccatga
     1261 tcacgatatg gcgtatctgc acggggatgg ggcggaagtg catgaccatc cacatgatca
     1321 cgagcatcat catgaccacg atcacacagg ggagcatcat caccatcatg agcaccgcgg
     1381 gctgccggag attcttgcga ttatccgcag cggcggtctg acaccgggag caagagcgct
     1441 ggcggagcgg attttccaga ttcttgccga agcggaagca aaagcccatg gcgtgccgct
     1501 tgatcaggtg catttccatg aggtgggagc tgtcgattcc atcgtggata ttgtgtctgc
     1561 cgctgtctgc ctggataacc tggcgccgga tgaagtcatc gtgacgggtc tcagcgaggg
     1621 aagcggcttt gtcagatgcc agcacggcat gatcccggtt ccggtaccgg ctgttttaaa
     1681 tatcgtacag gcacatggcc tgaccctcgt tccgaccgga atccagggtg aactggtcac
     1741 accgaccgga gcagccattg ttgcggcaat ccgtaccaaa gagacactgc cggcatcctt
     1801 caaatgtacc agaacaggtc ttggcgccgg aaaacggacg tatgagcgcc cgtcactgct
     1861 gcgtgcgatg atgctcgaaa cagaagaaaa cgacgagaaa gatacgatct ggaagctgga
     1921 gtgcaacatt gatgactgca ccggcgaggc gctcggctac tgcatgggaa aactgctgcg
     1981 atcaggcgca agagatgtcc attacatccc ggtctatatg aagaaaaacc gcccggctta
     2041 ccagctggat gtaatctgtg atgatacaac gcgggagacg ttggaaaata tcatattcgc
     2101 cgagaccacc acaatcggca tccgccgctg ccggatggag cgcactgtga tgaaacgcga
     2161 gtttgcgaca atcgcaaccg agtacggcga tgcgacagtc aagatctgcc gccacggcga
     2221 gatcgaaaaa atttacccag agtacgagag tgccgcagta attgcagaaa aaagcggcat
     2281 gccggtcggc gaggctggtg cgtggatcgt acagaaatat aaagaaggaa acaaaaaagc
     2341 atgagatatt taaaaccaca tttttacgac cagtttgtat gcaccgccgg agactgcccg
     2401 gatacgtgct gtgccggctg gcagatcgtg atcgacgagg attccctgga gcggtatgga
     2461 aatgagaaga gtgagtttgg aaagcgcctg cgcaattcga tcgactggga ggaagagtgt
     2521 ttttaccaga acaaccgccg ctgtgccttt ctgaatgatg aaaatctctg cgatctttac
     2581 aaggaactgg ggccggattc cttatgcgac acctgccgcc tttacccgcg ccatacggag
     2641 gagtatgagg gtctgcgtga attatccctg tccctttcct gcccggaagc tgcacggatt
     2701 atcctgtcct gcaaagagcc ggtgcgcttt ctggaggagg agaccgacga ggaggatgat
     2761 tttgaagagt ttgacttcat gatgttctcc cagctggaag acacgagaga tgttttgttc
     2821 cggattctgc agaaccgcga actgccgctg caggagcgca tgacggcggc agagcagctc
     2881 gcagaacagt atcagatctg tatggaggag cagcgggagt atgatatcga tgacctgctt
     2941 cggaagtatg aaaagcatct ggaagaggga accttaagcg agtgcgtggc ggagagcctt
     3001 gcggaaaaag gtgtggatgc tgcatccttt catgcatatg accgccaggt gaaggaactt
     3061 gccgttctcc gcggtctcga gcgtcttcgt ccggaatggg atactgtgct tgacggcaca
     3121 gaaaaagcgc tgtatcagaa cggagaaacc gcttatcagg ccatctgtga agaattccat
     3181 cgcacctggg gcgctgcagg tgaacgccgt gcagagtggg aaaacatcgg tgagcagctt
     3241 ctgatgtttt tcgtctacac ctatttttgc ggcgccgtct acgacgatat ggtctgttcc
     3301 aaaatggaac tcgcgctgtt ctctgtccgt tggattcagg aaatcctggt tgcacggtgg
     3361 ctggaaaatg gaaaaacgct ttccatgcac gacatcgagg agctttcctg gagatacgcg
     3421 cgcgaggtcg agcattccga cgataatctg aatgctcttg aggactggct ctttgaaacc
     3481 tatgcgccgg aaggatgcgt actggaagag gaatagccct gatatccgag gagttccttg
     3541 tacattttcc ccgatctgtg ctaggataaa gatgttgtac agaaagaaaa gagggaaaat
     3601 tatgcaggaa aaacaaaaga aatggattct gcgcattgcg ctttatctga ttggaattat
     3661 ggtgctggca ctcggcatca cactgaatac gaagacgaac ctcggcgtat cgcccatcat
     3721 ttccatgccg tacgcggtgg cacagatctt cggctatgaa acaggaacgg cggtcttcgt
     3781 ggcgtatttg atcttcatcg tactgcagat cgtactgctg aaaggaaaat tcccgccgtt
     3841 ccagcttctg caggttgccg caagttacct gacgagcgcc tttatcacca tcttcgaccg
     3901 gattctcccg gcacctgatc agatgggaat gcggcttctc gtccttgccc ttgccatcat
     3961 cctgaccgga attggcgccg ccgtcaccgt agacgtcaac atcgtcccaa acccggccga
     4021 cggcctggca agagttctcg gcgagcgctg cggaaagaac atgggtttcg gaaaaaatct
     4081 ttttgatttc tccgccatcg tcatttccct tgcgatcggc ctcatcggag ccggccgccc
     4141 ggtaggaatc ggaatcggca ctgttatagc catggtctgc acaggaagag ttatcgcggc
     4201 attcaatcat ttcgcaaaaa agaaaataga agaaatcacc atcgcctgag tgatccatgc
     4261 ccctgtcgaa aaaacttgag ttttcggtgt gggtttgtta taatgagaga cagcgaaaga
     4321 aaaatttttt gatttgtcat aaggcgtttg tttgcgttat gagaagtgcg aaggaggaag
     4381 tggcaaatga acttagttat tacgatgagc cgccggtttg gtactggggc gagtattatt
     4441 gcgaaagaac tttccgagag actgcatatc ccggtatatg ataaggacga tgtagagcac
     4501 ggcatgcgtg aaaatgcctt tgaatctgag gcggatgcga tccgcgagct ggcgaaacag
     4561 ccgtgtatta tcattggacg gtgtgcttcg gagtttctga aagataaaag caacgtcatc
     4621 aatatttatg tctgcgcgga caaagaggat cggatcaaac gtatcatgaa gctgttttcc
     4681 ctgacaagag aggcggctga ggtgatgctg gaagagacag acaagcagcg tgccgagtat
     4741 tattacaaaa ataccggaaa gacatggggc gatgtcaata actaccatat gatcctgaat
     4801 acctcggatc ttggcatcga gaactgtgcc gatattctga tgcggtattt tgaaatgaaa
     4861 gattatatct aagagatatt taaaaagaaa tacataaaaa atcaggtgct tcccgaaaaa
     4921 gagaagcacc tgattttttg tgcggtttag taagcctgat acgtattgcc ataactccgg
     4981 ctggagaagt aattctgttt cagaaccagg aacagttcgg catcggttgc atatttgtac
     5041 ggatacagat agaaaatacc gacaagattg caggtgattc cggaaagaat ataccatccg
     5101 ataaaggaga gatcaagaat aaatgtattc atcttttcac cgctcatcat ttcacggctg
     5161 atgcggaatg cttcttcggt cgaaagttcc gggtagtcgg cgagaagata aggaaccatg
     5221 tggtattcat aagctttcac aattccagga atcagaagaa gcagcgacca gaggaatata
     5281 taaagatttt ttaaaaacag agtccacaca gtttttccat agtagccgtt ctggaatccg
     5341 aacagtatgt tgccaagtcc cgctttttcc attgagtttt caatgaaaaa cctcgagcct
     5401 cctacctcaa atggtgcaag aacaaaaata cggatcagaa taaaggcaag accaatgaca
     5461 agaagtacaa tgccgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa acaaatttaa
     5521 ccggtccaag cggggtgaat ctttctgcaa aggattccac attgaagtta tcggaagaaa
     5581 tcgtaaatcc agatataccg accgagttag tctgatggct gtcattgttg gaatttccag
     5641 aattagaagt accgcttccg accagcagag tcagaatcag ggcggctacc acacagatcc
     5701 agtaattgcg taaaaatgcg gcttttccgc gggttttcag ttcctctcga gtccacataa
     5761 aaaagacctc cctcttaatg taagtgtaaa cagcagacaa ttctgccaag tcacgattat
     5821 cttaccatgg ggcaggtgaa acatcaagac agcttaagga aagtttaaga aattggcaga
     5881 aagattaaag tggaaatgtg cattcttaaa taaagatttt acttgtgaag cggggatgaa
     5941 tgtgctataa tctctccggt gcgtcagaac gcagcggggc cgggtttctc cggggtgcgt
     6001 tctgaaaaaa aagaaacctg tacgttgaat tgacaaagag agcgaggaat ctacgtgtat
     6061 ctgacaaagg gcgagtttct gcagaaattt gggattctgc cgcaggaatt tgaagaggca
     6121 gatatttcct gggaagaact gctggaaatt gcggatgatt acgaacggcg ccggccgacg
     6181 ctggaaaaga tccgaaaaga attcgtagca gaatttctgc aggacaagga aaaagagatc
     6241 ggactgcagt cgtaccacag ccgcctgaag gacaccgaac atctggtgga aaagctggtg
     6301 cgcaagcgtc tggaaaatta tgcaaaatac cggaaaatgg atgcgacaaa ctacatgcgt
     6361 tatgtgacag acctgatcgg aatccgggga ctcttgctgt atcgggagga ctgggtaaac
     6421 ttccataaat acattatcca ctggtttaaa aacgatccgg aaaaatacat ccgtgattac
     6481 ggccgtgact acgaccagaa cgcatcgggc tatatggcag agccgccgaa agtccataca
     6541 agacttggcg attacgccga tatttatgtg aactggattc cggaggagaa cattctggac
     6601 cggaaacatt accgtgcggt gcattacatt gtcgtttacc gcggcgtcta cattgaaatt
     6661 cagattaaga cgctgttcga ggagggctgg ggagagattg accacagcat tctttatccg
     6721 cggcgcaagg gaaacgccat gctgacagag ttttccgagc tgctgaaccg tctggcgggc
     6781 atgggagatg agatgggatc gttctaccgc cgtctccagg ttgtgccgga tgagaagttc
     6841 cagagcaagg aaacgatcgt gcgcaagcgt gaattaaaac cgcaggtcaa ggcagcggtg
     6901 gaaaaacgcg atctgaacga aattcatacg atggatgatg ccgtctggag catactgaaa
     6961 gaataaaact gcttttcaga catgggaaag gataaaaaat ggatggaaga aacacgggcc
     7021 gcatgggctt ctctcatgca gcagaggact ctgcggagaa tatgatggcg gcagtcaaag
     7081 ccggaaaaat ccgaaaacat ctgatgggaa atacctcgcc gatccgctac ggaacaatgg
     7141 aaaaacgcga gatgaccgaa tataccgact ttgtccggac gaaactctgc tatgaaatcc
     7201 tgaatctgcc cggggaggaa aaatgaatac aatagaaaaa atatacacaa actacgacgg
     7261 acttctggaa gaattttccg aggaagtaat ccagagccgt tacgctgttt tttacgaaga
     7321 aatcgaggaa tttgccaaga gtctcggcat ccgcgagaaa atccagatca gcgagtcgct
     7381 gttgagccat gcagtgctgg actatttcac ggatatttcc cgcctgaaac attttcatca
     7441 ggcaaagcat atcaattcat tgaaagtgat ctcctatgag acctactggc tgctgcggag
     7501 aaaaccgatc cagatcctgg tggaggatga gacatcggat gcgatggcat ttctgaacga
     7561 aaagttcgtc ttttcccgga ttgccaaata tctgatgggc gacggaaaac gggtgatttt
     7621 aagtccggag acgaaaaaag gctttctgaa ttatctcgat tcgctgtttt attacctgaa
     7681 ataccgcaat tacgatgcgg aaatgctcga gatgatgctg atgggcttca aagcgggtgt
     7741 tctcgtggcg gatgatttaa aggagcagga gtcctgaaaa acgggcggaa gcggaataca
     7801 acaaaataag atgaagatcg aaagaaccgg acgggtcgta aggctgctgt cgggttcttt
     7861 ttttgtgcgg tgctgcataa agtgcagaga aaacattgcg atgcatggat agaaaactgt
     7921 ttaacaatgg agggacggga actgctttcc tttggaactt tgtacaggga ggaatggata
     7981 tggccacgct cgaaaaaatc aatgcatttc taaaagcggt gaatgcagcc gcctggggac
     8041 cgccgcttct ttttctgctg cttgggacag gcatttttct gaccatccag cttcgcgcgc
     8101 tacccattcg acggttgttc tttgcgcttc gtacagcatt cggccgtgag gcgaggtaca
     8161 gcggggagaa aacagaagcg ggagtttcgc cgttcgcatc gctgatgacg gaacttgcgg
     8221 cggagatcgg gacgggcaat atcgtggggg ttgcctccgc aatggcgctt ggaggaccgg
     8281 gagcactggg atggatgatg ctggcggcag ttgtgggact tgctttgaaa ttttctgaga
     8341 gttttctctc ggtgcagtac cggatgcaaa gaaaagacgg aacatactgc ggcggtccga
     8401 tggtgacgct gaccagagca tttccggggg agcggctggc gaagatgctg gctgtgtttt
     8461 ttgccggaag cgccgtgctg gtatctctcg gaatgggcaa tatgacgcag gcaaactcga
     8521 ttgcgctggc gatgcaggat acgtttggcg tggcaagaga aaaaagcgcg ttggtgctga
     8581 cgattcttct tctgctcgtg gtgacaggcg gactgcgttc cattgcaaaa gcggcggagg
     8641 tgctggtacc gggaatggca ctcttttatc tgggcggggc aggaatggta atcggtctgc
     8701 acgccggaga gcttccgcgg acactgcttg cgatagtacg ggacgcgttt tctttccggg
     8761 cggcatcggg aggagcggtg ggtgcgggaa tttccctgct gccgccggcc ttgcggtatg
     8821 gaatttcccg cggtgtgttt tcgaatgaag cagggcttgg agcaggagga atcagtgctg
     8881 cggcggcaca gaccgacgat cccgtgcggc aggggtacat cagcatgaca ggtgtgttcg
     8941 ttgataccat ggtgatctgt cttgtgaccg gccttgccgt tggagtgacc ggggcggcag
     9001 agggggtact ggaggcggaa aaagcggatg gagcggcaat ggtaattcag gcctttgaaa
     9061 gtgtgttcgg accggcggga ggctatgttg tcgcggtcgg gattctgctg ttcgcatttg
     9121 caacgatggc cggctgggca taccaggggg agcaggcgtt tctctggctg gtgaaaaagg
     9181 atagcttcgg aatggtttac cgtgttttct tctgctttgc agcgtttgcc gggtgcgtct
     9241 gcatggcgga aacggtctgg aattttgcgg agcttgccaa tgcctgcatg gcggtgccga
     9301 atctgctctg tgtgctgcgg ctctggaagg aagtaaaaga ggaggcattc cggtttgaaa
     9361 gcaggataaa aaaagcagaa caaaagaaag atagaaatac atgagaaacc gtatttccgg
     9421 tcctcaggca gatttcgatg cgagctgatc gagagagccg aaagaatatc ccatttcttc
     9481 ccatttcgtc agcagctcat cgagaatctc cgcgttggtg gatgaggtat tatgaagcag
     9541 gacgatggca ccgggatgaa tgcgtccggt caactttttg aaagcctcct cgtgggtcgg
     9601 ctgctgatcc tggtaccagt ctacataggc gagactccag aaaaacgtat ggtatccgag
     9661 gtcctttgcc atctgcaggt tctgtgtgct gtattttccc tgcggcggcc ggtaaaattt
     9721 caccatttcc tggccggtgg tctcctgata aagtgtggag acgcggttca gttcttcgga
     9781 gaaggcttcc tgcgtggaga ttttggacat atccgggtgc gaccagg
//
LOCUS       DAOMUF010000069         9778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1711_length_9778_cov_5.095958, whole genome shotgun sequence.
ACCESSION   DAOMUF010000069 DAOMUF010000000
VERSION     DAOMUF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1711_length_9778_cov_5.095958"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLN77_05475"
     CDS             complement(<1..617)
                     /locus_tag="PLN77_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM43762.1"
                     /translation="MARGIDTKCIHLEEDEGQCSHYGALSYPIYQTATYAHPGVGQST
                     GFDYSRLQNPTREHLEKMVAALENGIDAFALSSGMAAISLLMEMFRQGDHIIADSDLY
                     GGSIRLFHNVNEKNGVEFSSIDCCRDDVESYVKENTKAIYIETPTNPMMNVTDIRKMA
                     DIAKRYNLILIVDNTFMSPYFQNPLDLGADVVIHSGTKYLSGHNDT"
     gene            complement(819..1580)
                     /locus_tag="PLN77_05480"
     CDS             complement(819..1580)
                     /locus_tag="PLN77_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRM43763.1"
                     /translation="MKFVTFNIRCDFGQDGANNFIYRRPLILEKIRQEKPDIIGFQEV
                     LPHVAAWLKENLTEYYIAGCGREKDLTGEAMIIAYRKERFQSVSLETFWLSPTPYVPG
                     SRYPVQSMCPRTATDVVFEDMETGKVFRVINTHLDHECAGARKLGLEQILAHLRAEKA
                     FADVPVILSGDFNAEPDSEEMKPLRETAGLVNATEGIGITFHNYHRDDPNDPQCSIDY
                     IILKGDWELKKVEKWTEQKDGVCLSDHYPICAELI"
     gene            complement(1810..2130)
                     /locus_tag="PLN77_05485"
     CDS             complement(1810..2130)
                     /locus_tag="PLN77_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43764.1"
                     /translation="MVGSPDSVQKNVVLENNSLSAEQITGVENRKSKAGEAVLPVLRS
                     VSLFLLGLLGVLLILLTLLVIAAEAARIICLGPAAKKQQQPMQNSPADFRRSSQANIR
                     VSAI"
     gene            complement(2269..4011)
                     /gene="ilvD"
                     /locus_tag="PLN77_05490"
     CDS             complement(2269..4011)
                     /gene="ilvD"
                     /locus_tag="PLN77_05490"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM43765.1"
                     /translation="MLKSQEMRKLAPEMDPLRIGTGWKKEDLEKPQIMVESTYGDSHP
                     GSGHLNLLVEEVRKGVAEAGGFGARYFCTDICDGESQGTDGINYSLASREMIANMIEI
                     HANATPFDGGVYLSSCDKGMPGNLIGLARVDIPAVVVPGGTMNAGPEMLTLEQLGMYS
                     AKFERGEIDEEKLDWAKCNACPSCGACSFIGTASTMQIMAEALGLALPGSALMPATSP
                     DLLAYAREAGRQAVKLAQMEHMRPSDLVTKESFENAILVHAAISGSTNCLLHIPAIAH
                     EFGIEITGDTFDRLHRNARYLLDVRPAGRWPAECFYYAGGVPAIMEEIKDHLHLDVMT
                     VTGKTLGENLAELRENGFYEKCSGWLAKFNERYGTSITKEDIIRPYDKAIGTDGSIAV
                     LRGNLAPEGAVIKHTACPKEMFHAVLRARPFDSEEECLDAVLKHKVQKGDAVFIRYEG
                     PKGSGMPEMFYTSEAISSDKELGKSIALITDGRFSGASTGPVIGHCSPEAVDGGPIAL
                     VEEGDLIEIDVKERKLNIIGIAGERKSMEEIDAILKERRQNWKPREPKYKKGVLRLFS
                     LHAASPMKGAYLEY"
     gene            complement(4151..4948)
                     /locus_tag="PLN77_05495"
     CDS             complement(4151..4948)
                     /locus_tag="PLN77_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43766.1"
                     /translation="MAAELFLGLLVLGCLFCGQIMTKDPSYMDLKNCSVMPCREFLFG
                     TDTMGRDIFSMIWYGGRISLAIGLLSTLISTVIAVLFGAVSGCAPAWLDALLMRLTEI
                     FLSVPNLLLVIFLLAAAGKSNVITISAVIGMTSWTSIAKVVRTEVRQIRNSEYVIASR
                     CMGGSFFYILWHHLAPNFFSSIMFMVVMNIRSAIVAESTLSFMGIGLPLEVISWGSML
                     SLSEKALMTKAWWIILIPGVFLIVTLLSFTSIGNYLRKQANRKQRNL"
     gene            complement(5041..6048)
                     /locus_tag="PLN77_05500"
     CDS             complement(5041..6048)
                     /locus_tag="PLN77_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43767.1"
                     /translation="MKSRNRVLYYGKKAAAFLASVLLLSVLVFYISRLAPGDPLVSYY
                     GDRVEKMTPEERAWAEEKLGLKDPISVQYIRWAGNALHGDFGISYKYKMDVTRVIEGR
                     IGNTLVLGGIGFLLIFTLALLLGILCAWKEDTWLDRIICKVGTITGCIPEFWLSLLLI
                     LVFSMQLKWLPSSGAYSVGKQADFSDRVCHLILPMTVVVLSHLWYYAYMIRNKILDEV
                     REEYVLLARAKGLKRKTVLFRHCLRNTIPSYLGIMAISVPHVLGGTYIVETVFSYPGI
                     GTLAYESARYKDYNLLMLLCLMSGIVVILCNMAAQIINERIDPRMREKEAAETSEVMM
                     L"
     gene            complement(6045..6950)
                     /locus_tag="PLN77_05505"
     CDS             complement(6045..6950)
                     /locus_tag="PLN77_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM43768.1"
                     /translation="MEHEILLDVRHLTHIFPLSRKTAVKAVDDVSFQIRRGEIFGLVG
                     ESGSGKSTAARCVMNIYQPYAGQIYYKGIDTCDRGQFRKNKKMLQTTRQMIFQDSGSS
                     LNQKMKVRDIIEEPMRVQKIRPLRGSYEEEARFQMKYVGLDPEYLKKYPGELSGGQRQ
                     RVAIARALTMEPELLVADEPIASLDASIQAQIVNLFRHLQKEHGFSFLFIAHDLDMVE
                     FLCDRVGVMYHGKLVETGPARAVFENPLHPYTKALIAAIPIPDPRRERARAVPDFSNT
                     EFPRTGTLREVEKDHFVLMEGGDAG"
     gene            complement(6940..7956)
                     /locus_tag="PLN77_05510"
     CDS             complement(6940..7956)
                     /locus_tag="PLN77_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43769.1"
                     /translation="MKERLKVEHLSVSFFTDEGEVQAVRDVSFSLNAGEVLAVVGESG
                     CGKSVLCKSIMKLLPENAKIKNGKILVNGEEIAGYGERRMRELRGKLFSMVFQNPMTS
                     LNPTMTVGAQIAEAVRAHQKDMGKEEVEQRVTELMQLVGIEQAEERKKAYPYHFSGGM
                     RQRIVLAIALAGNPEILFADEPTTALDVTIQAQILDLLREIQKKLGTATVFVTHDLGV
                     VARIADRVAVMYAGKIVEIGTVDEIFYDPRHPYTWGLLRSIPAFSRGKESLPSIPGMP
                     PSLLHPPKGDAFASRNAYALEIDFEEEPPMFRVTDSHYAATWLLDERAPKVVPPIGGN
                     TDGT"
     gene            complement(8021..9622)
                     /locus_tag="PLN77_05515"
     CDS             complement(8021..9622)
                     /locus_tag="PLN77_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43770.1"
                     /translation="MKKKILSLMLMVSLAASLAACGQDSGKNSNSTDLSKTETEASSG
                     QTGEEETTLVYGSADYTRINPAMDEHGEINLLLFNGLTAHDADDNIVPGLAEKWEYDA
                     ETCTYTFHIRDGIQWHDGEKFTADDVKFTIEAIMDPENGSENAPNYEDVEEITVIDDS
                     TVSFRLSEPNVAFLEYMTMAILPKHLLEGENMQESDFFRAPVGTGPYKLESWDEGQSI
                     VLVKNEDYYLGAPKIDKVIFKIVPDDNAQAMQLESGELDLALLDPKNAQNFKEKDGFT
                     CYDMTTADYRGILFNFANDYWTKNRDIIPAVCYSIDRQAIIDSVLLGEGMAAYSPLQR
                     NIYNNENVEHYDYNPEKAQEILEAAGCTRNSDGFYERDGEEIGFVISVMSGEQDRIDI
                     AQAAAQQLRETGINCTVEIPAQMDWGGQMACLIGWGSPFDADDHTYKVFGTDKGANYS
                     SYSNEKVDEYLSLARQSDDPEVRKEYYGKFQEELAEDPAYAFICYIDANYVADSGIQG
                     ISKDTVLGHHGVGIFWNIQDWTIEE"
BASE COUNT         2193 a         2802 c         2127 g         2656 t
ORIGIN      
        1 gtatcgttgt ggccgcttaa gtatttcgtg ccgctgtgga tgacaacatc tgcgccgaga
       61 tcgagcgggt tctggaagta cggggacatg aaggtgttgt ccacgatcag aatcaggttg
      121 tagcgttttg caatatccgc catctttcgg atgtcggtta cgttcatcat cgggttcgtc
      181 ggagtttcga tgtagattgc ttttgtattt tctttcacat agctttccac gtcatcacgg
      241 cagcagtcga tgctggaaaa ctcgacgccg tttttctcgt tgacgttgtg gaacagacga
      301 atgcttccgc cgtacagatc ggaatccgca atgatgtggt cgccctggcg gaacatttcc
      361 atcagaagag agatcgctgc catgccggag cttaatgcaa acgcatcaat tccgttttcc
      421 agtgcagcca ccatcttctc cagatgctct ctggtcgggt tctgcagtct gctgtaatca
      481 aatccggtgc tctgtcctac gcccggatgc gcatacgttg cggtctgata aatcgggtag
      541 ctgagtgcgc cgtagtggct acactgtcct tcgtcttctt ctaaatggat acattttgtg
      601 tcaattcctc ttgccatatg aataatctcc tgttatttcg taatttttct ttctatacta
      661 atctgatagg ttttgtaacg taacctatca taacagaaaa tgcggcgttt gtatattccc
      721 ttttttcttt gacttgttat aagtttttcc tataactatc gtaaaaaaac gccgccagaa
      781 gattttttct cccggcggcc gcctcttgca tccatatttt aaataagttc cgcacagatc
      841 ggataatgat cagaaaggca cacaccgtcc ttctgctccg tccatttttc cacctttttc
      901 agttcccagt cccctttgag aatgatgtag tcaatcgagc actgcggatc attcggatca
      961 tcgcgatgat agttgtggaa ggtaatgccg attccttccg tcgcattcac aagaccggca
     1021 gtttccctca gcggtttcat ctcttcgctg tccggctcgg cattgaagtc gccgctaaga
     1081 atcaccggca catcggcaaa tgctttctct gctctcagat gtgcgagaat ctgctccagg
     1141 ccaagctttc tcgccccggc gcactcgtga tccagatggg tgttgatgac gcggaacact
     1201 tttccggtct ccatatcctc aaataccaca tccgtcgcag tacgcgggca catactctgt
     1261 accggatagc gggatcccgg cacgtacggt gtcggtgaca gccagaaggt ttccaggctc
     1321 acactctgaa aacgttcttt ccgataggca atgatcattg cctcaccggt cagatccttt
     1381 tctcttccac atccggcaat gtaatattcc gtcagatttt ccttcagcca ggcagccaca
     1441 tgcggcagta cctcctgaaa accgatgata tccggctttt cctgtctgat tttttccaga
     1501 atcagcggtc tacggtagat aaaattattg gctccatcct ggccaaaatc acagcgaata
     1561 ttaaatgtta caaatttcat ggtaaaatcc ctcttttcat ttgttttcct aacccttatt
     1621 tttgctggaa agcgctgtgc tttctcttct ttttcttcgt attcacacgg cgcagacacg
     1681 catagcgcag cttcagccgc attttccagc tgcagcccgg taccggctcc atcagctttt
     1741 ccagaaaatg gttcagcgta cgttcctcat aaggagacag cagaatctca ccgtagatat
     1801 gtttctcgat cagatggcag acacgcgtat attcgcctgg ctcgaccgcc ggaaatctgc
     1861 gggcgagttc tgcatcggtt gctgctgttt ctttgccgcc ggtccaaggc agatgatccg
     1921 cgccgcctcc gccgcaatca caagcagcgt cagcagaatc agaagcacgc caagcagccc
     1981 gagaagaaac agcgaaacac tgcgcagtac cggaagaacg gcttcgcctg ccttgctctt
     2041 tcggttttcc acaccggtaa tctgctccgc cgacagcgaa ttgttctcca gcaccacatt
     2101 cttctgtaca gaatctggac ttccgaccat tttctgaaga gatgcaacat tatacaggaa
     2161 cagtttgttt tttaggtatt ttctttcact tgtttttctg ttgctccttc ctccattttt
     2221 tgctctgcct taacttctaa agaccatccc agaaaaacgc aagtattttt agtattccag
     2281 atacgcccct ttcatcgggc ttgctgcgtg gaggctgaaa agacggagta cgcctttttt
     2341 gtatttcggc tcgcgcggtt tccagttttg gcggcgttct tttaagatcg catcgatttc
     2401 ttccatggac ttgcgctcac cggcgattcc gatgatgtta agttttcgtt ccttcacatc
     2461 aatctcaatc aaatcccctt cctcgacgag tgcgatcggg ccgccgtcta cagcttcggg
     2521 actgcagtgg ccgatgaccg ggccggtgga ggcgccggag aaacggccgt cggtgataag
     2581 cgcgatgctc tttccaagct ctttgtcgct gctgattgcc tcggaagtat agaacatctc
     2641 cggcataccg ctgcctttcg ggccttcgta gcggatgaag acggcgtcgc ctttctgaac
     2701 cttatgtttc agcacggcat cgaggcactc ctcctcgctg tcgaacggtc gggcacggag
     2761 gacggcgtgg aacatttctt tcgggcaggc cgtgtgcttg atgacggctc cctccggcgc
     2821 aaggtttccg cgcagaacag cgatgctgcc gtccgttccg atcgccttgt catacgggcg
     2881 gataatatct tcttttgtaa tcgaagttcc ataccgctca ttaaattttg cgagccatcc
     2941 gctgcacttc tcataaaatc cattttcacg aagctccgcc agattttcac ccagtgtctt
     3001 tccggtcact gtcatcacat cgagatgcag atgatctttg atttcttcca taatcgccgg
     3061 aacgccgccc gcataataga agcactctgc cggccatctt cccgccgggc ggacatcgag
     3121 cagataccgc gcgttgcggt gcagacgatc gaatgtgtcg ccggtaatct cgatgccgaa
     3181 ctcatgcgca atggccggga tgtgcagcag gcagtttgtg cttcccgaaa ttgccgcgtg
     3241 gacaagaatc gcattctcaa aactctcttt cgtcacaaga tcgcttggcc gcatatgctc
     3301 catctgtgcc agttttaccg cctggcggcc tgcctcccgt gcatatgcca gaagatccgg
     3361 gcttgtcgcc ggcatcagag cgcttccagg cagggcaagg ccgagtgcct ccgccatgat
     3421 ctgcatcgtg gatgccgttc cgataaacga acaggcaccg cagctcgggc aggcattgca
     3481 tttcgcccag tcgagcttct cctcgtcgat ctcaccgcgc tcaaattttg cgctgtacat
     3541 gccaagctgc tccagtgtca gcatctccgg tcccgcattc atcgttccgc ccggcacaac
     3601 aaccgccgga atatcgacac gcgcaaggcc gatcagattg cccggcatac ccttgtcgca
     3661 gctcgacaga tagacgccgc cgtcaaacgg tgtcgcattc gcgtggatct cgatcatatt
     3721 ggcgatcatc tcgcggctcg caagactgta gttgatgccg tccgttccct ggctctcgcc
     3781 gtcacagatg tcggtgcaga aataccgcgc cccgaagccc cccgcttccg caacgccttt
     3841 ccgcacttcc tcgaccagca gatttaagtg gccgcttccc ggatggctgt ccccgtacgt
     3901 actctccacc ataatctgcg gtttttcaag atcctctttt ttccatcctg ttccaatccg
     3961 gagcggatcc atctccggtg caagtttccg catttcctga ctcttcagca tttccttctc
     4021 ctcccgctgt tttttaggta ttaaaaatac tctttctgct tttcactata gcaatccctg
     4081 tatttgattg caagacaaaa aagctgcagt ctgcagggat tccccacaga ccgcagcctc
     4141 tctgaacagc tcacagattt ctctgttttc tgtttgcctg cttccgcaga taatttccaa
     4201 tgcttgtaaa actaagcagc gtcacaatca gaaaaacacc cgggatcaga atgatccacc
     4261 atgcctttgt catcagcgct ttttccgaaa gtgacagcat gctgccccag gaaatcacct
     4321 ccagcggaag accgattccc ataaaactca gtgtggattc tgctacaatc gcacttcgga
     4381 tgttcataac caccataaac ataatagacg aaaaaaagtt cggtgccaga tggtgccata
     4441 aaatatagaa aaaactgcct cccatacagc gcgacgcaat cacgtattca ctgttccgga
     4501 tctgccgtac ttcggtccgc acaacctttg caatgcttgt ccagcttgtc attccgatca
     4561 cggcagaaat cgtaatcaca ttggattttc ccgcagcggc cagaagaaaa atgacaagaa
     4621 gaagattcgg caccgacaaa aagatctcgg tcagccgcat cagaagtgcg tccagccacg
     4681 ccggtgcaca tccgctgact gcgccgaaga gaacggcaat cacagtggaa atcagcgtag
     4741 aaagcagacc gatcgccagc gatattctgc cgccgtacca gatcatcgag aaaatatctc
     4801 ttcccatcgt atcggttcca aacagaaatt cccggcacgg catgacggaa cagtttttca
     4861 gatccatata cgagggatcc ttcgtcatga tctgcccaca gaaaaggcag cccagcacaa
     4921 gaagacccag aaaaagctct gctgccaccg gccggtcctg ataccatttt ttcttttttt
     4981 ctgcttctgt tttttctgca ggcctctctc ctgcaaattg aaataattgt gttttttctg
     5041 tcacagcatc attacctccg atgtttctgc cgcttctttc tcccgcatcc gcggatcaat
     5101 tctctcgttg atgatctgtg ccgccatatt gcacagaatc acaacgattc cggacatcag
     5161 gcaaagcagc atcagaagat tgtaatcttt gtaccgtgcg ctctcatacg ccagcgttcc
     5221 gataccggga tacgagaaaa ccgtttctac aatataggtt ccccccagca catgcggaac
     5281 agaaatggcc atgatgccaa gatacgacgg aatcgtattc cgcaggcaat gccggaacag
     5341 aaccgtcttt cgtttcagcc ccttggctct tgccagaagc acatactctt cccgcacttc
     5401 atccagaatt ttattgcgga tcatgtatgc gtaataccac aaatgactca gcactacgac
     5461 cgtcatcggc aggatcagat gacagacacg gtcagaaaaa tctgcctgtt ttccaacaga
     5521 gtaggcaccg gaactcggca gccatttgag ctgcatggaa aaaacaagaa tcagaagcag
     5581 cgacagccag aattccggaa tacatcccgt gatggttccc accttacaga tgatgcggtc
     5641 cagccaagta tcttccttcc atgcacagag aattccaaga agcagcgcga gggtgaaaat
     5701 cagcagaaat ccaatgccgc caagcaccag cgtatttcca attcttccct cgatcacgcg
     5761 cgttacatcc attttatatt tgtacgaaat tccgaaatcc ccatgcagcg cattgcctgc
     5821 ccagcggata tactgtacgg aaatgggatc cttcagtccc aatttttctt cggcccacgc
     5881 tctttcttcg ggcgtcattt tttccacgcg gtctccgtaa tatgatacca gcggatctcc
     5941 cggcgcaagc cttgaaatat aaaatacgag cacagacagc agcagaacac ttgccagaaa
     6001 tgccgctgct ttttttccat agtaaagcac tctgttccgg cttttcatcc tgcatcgcct
     6061 ccctccatca gcacaaaatg atctttttcc acttcccgca gtgttcccgt ccggggaaat
     6121 tctgtattgg aaaaatccgg tacagctctc gctctttccc ttcgcggatc cggaatcgga
     6181 atggccgcga tcagtgcttt cgtatacgga tgcagcggat tttcaaacac ggcccgcgcc
     6241 gggccggtct ccaccagttt tccatggtac atcacaccta ctctgtcaca cagaaattcg
     6301 accatatcca gatcatgcgc aataaacagg aacgaaaaac cgtgctcctt ctgcagatga
     6361 cggaacagat tcacaatctg tgcctgtatc gacgcgtcca gcgatgcaat cggttcatcc
     6421 gccaccagaa gctccggctc catcgtcagt gcccgtgcaa tcgcaacgcg ctgccgctgt
     6481 ccgccggaca attctcccgg atattttttc agatattccg gatccagccc cacgtatttc
     6541 atctggaatc tggcttcctc ctcatagctt ccccgcagcg gccggatttt ctgcacgcgc
     6601 atcggctcct caataatgtc gcggaccttc atcttctgat tcagactgga accggaatcc
     6661 tgaaaaatca tctggcgtgt cgtctgcagc atttttttat ttttccggaa ctggcctctg
     6721 tcgcaggtat caatcccttt ataataaatc tggccggcat atggctgata aatattcatc
     6781 acacagcgtg cagcggtaga ttttccggaa ccggattctc ctaccagtcc aaaaatttct
     6841 cctcttcgaa tctgaaacga tacatcatcg actgccttga ctgctgtttt ccgggacagt
     6901 ggaaaaatat gggtcagatg acgcacatcc agcaaaatct catgttccat ctgtgtttcc
     6961 tcctatcggc ggcacaacct tcggtgctct ctcatccagc agccatgttg ccgcataatg
     7021 gctgtctgtg acccggaaca tcggcggctc ctcctcaaaa tcaatttcca gcgcataagc
     7081 attgcggctg gcaaaggcat cgcccttcgg cggatggaga agagacggcg gcattcccgg
     7141 gatagacggc aggctttctt ttcccctcga aaacgccggt atggaacgca gcagtcccca
     7201 cgtatacgga tgacggggat cataaaaaat ctcatccacg gttccaatct ctactatttt
     7261 tcccgcatac atgaccgcta cccggtctgc gatccgcgct acaacgccaa ggtcatgtgt
     7321 gacaaagacc gttgccgttc ccagtttttt ctgaatttcc cgaagcaggt ccagaatctg
     7381 tgcctgaatc gtcacatcca gcgctgtcgt cggctcatca gcaaacagaa tttccggatt
     7441 gcccgccagc gcaatcgcca gtacgatccg ctgccgcatt ccgccggaaa agtgatacgg
     7501 atatgccttt ttccgctctt ctgcctgctc gattcccacc agctgcatca gttctgtcac
     7561 tctctgttcc acttcttctt ttcccatgtc tttctgatgc gcccgcactg cctctgcaat
     7621 ctgcgctccg actgtcatcg tcggatttaa ggaagtcatc ggattctgga aaaccatcga
     7681 gaacagtttt ccccgcagtt cccgcattct tcgctccccg tatcctgcaa tttcttctcc
     7741 attgaccaga atttttccgt ttttgatctt tgcattctcc ggcagcagct tcataatgct
     7801 tttgcagagc acactttttc cgcagcctga ctcgcctaca acagcgagca cctcacctgc
     7861 attcagggaa aagctgacat cgcggaccgc ctgtacttcc ccctcatccg taaaaaagct
     7921 gacggaaaga tgttccactt ttaatcgttc cttcattccg tttttctcct gtacagacaa
     7981 aaaggaggta accatacctc cttcgtctgc ttttttgttt ttattcttcg attgtccagt
     8041 cctgaatatt ccagaaaata ccaacgccgt ggtggcccag caccgtgtct ttggaaatgc
     8101 cctgaatacc ggaatctgcc acataatttg catcaatata acagatgaat gcataggccg
     8161 gatcttctgc cagttcttcc tggaattttc cgtaatattc tttccgcact tccggatcgt
     8221 cactctggcg ggcaagagac agatactcat ctactttttc attggaatag ctcgaataat
     8281 tcgccccttt gtccgtgccg aaaaccttat atgtatgatc gtccgcatcg aacgggcttc
     8341 cccagccgat caggcaggcc atctgtccgc cccagtccat ctgtgccgga atttctacgg
     8401 tacagttgat tccggtttcc cggagctgct gtgctgctgc ctgggcaata tcaatgcggt
     8461 cctgctcacc ggacatcacg ctgatgacaa aaccgatttc ttcgccgtcc cgctcataga
     8521 aaccatcgct gtttctggtg catccggccg cttccagaat ttcctgtgct ttttccggat
     8581 tgtaatcata atgttctacg ttttcgttat tgtaaatgtt ccgctgcagc ggactgtatg
     8641 ccgccattcc ctcgccaaga agcacagaat cgataatggc ctggcggtca atcgaataac
     8701 agacggccgg aatgatatcc ctgtttttcg tccagtaatc attcgcaaag ttaaataaga
     8761 ttccgcggta atctgccgtt gtcatgtcgt agcaggtaaa accgtctttt tctttgaaat
     8821 tctgcgcatt tttcgggtca agcagcgcca gatccagttc gccggattcc agctgcatcg
     8881 cctgtgcatt atcatctggt acaattttga aaatgacctt gtcaatcttt ggtgccccaa
     8941 ggtaataatc ttcatttttt accagaacaa tcgactggcc ttcgtcccag ctctccagtt
     9001 tatacgggcc ggttccaacc ggtgcacgga agaaatccga ttcctgcata ttttcacctt
     9061 ccagcaggtg cttcggcagg atggccatcg tcatgtattc caggaaagcc acgttcggct
     9121 cggaaaggcg gaaggaaacg gtgctgtcat ctataactgt aatctcttct acatcctcat
     9181 aatttggtgc attttcagat ccgttttccg gatccatgat cgcttcgatg gtgaatttca
     9241 catcgtccgc cgtaaatttt tctccatcat gccattggat gccgtcccgg atatgaaagg
     9301 tgtaggtgca ggtttctgca tcgtactccc atttttctgc cagacccgga acaatgttgt
     9361 catccgcatc gtgtgcggtc agtccgttga acagcagcag attaatttca ccgtgctcat
     9421 ccatcgccgg attaatcctg gtataatcag cagaaccgta aaccagggtc gtctcttcct
     9481 cccctgtctg gccggaagaa gcttccgtct ctgttttcga caaatctgta ctgttgctgt
     9541 ttttcccgct gtcctgaccg catgccgcaa gggatgctgc aagagacacc atcagcatca
     9601 aagataatat tttctttttc atccttttcc tcccattttc agaccgtttt tacccggact
     9661 gaccgctttt ttgctttgta ctcccatgat ctgcgcgcct gatccatgta ctgttcgttc
     9721 attatatttg atatttcagg ctctcacaag ccgggtgggg ggatattttt ttcttttc
//
LOCUS       DAOMUF010000070         9724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1722_length_9724_cov_3.781570, whole genome shotgun sequence.
ACCESSION   DAOMUF010000070 DAOMUF010000000
VERSION     DAOMUF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1722_length_9724_cov_3.781570"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..778)
                     /locus_tag="PLN77_05520"
     CDS             complement(59..778)
                     /locus_tag="PLN77_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HRM43771.1"
                     /translation="MEKLEQEMNAPEKMAETPSPKKTAGKKKKFRKNQVIITALAIMI
                     AVAGYINYADSTLSTKGTADGTTADTSTVLKDISSLDTDITDEVDAGAAGDSSRTITE
                     TPGEAVLAGASTYMAQARIDREQIRSQNKDTLNEIINNTNLSETERQSAVQSMVEMTE
                     LVEKESATELLLEAKGFSDVVVNLTGETADIVVPMKEVSEEQRAQIEDIVTRKTGIGV
                     ENIVITPMSSAADTAAEESAQ"
     gene            complement(792..1361)
                     /locus_tag="PLN77_05525"
     CDS             complement(792..1361)
                     /locus_tag="PLN77_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HRM43772.1"
                     /translation="MREKAAAFFRKQKKEQWMLYFLLAAIGVVLLLPTGTKKTGEETE
                     QPQETAARPAGDAGLSVQEEMEQRLCDALSQMDGVGAVHVVVTLESTGKKIVEKDVPT
                     RSTTEENKKGEETGSVTSSSQDEATVYEKTQNGAETPYVVSEEYPAVRGVLVIAEGGG
                     NPVTIQEIQEAVMALFQVGANKIKVVKMK"
     gene            complement(1366..1920)
                     /locus_tag="PLN77_05530"
     CDS             complement(1366..1920)
                     /locus_tag="PLN77_05530"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HRM43773.1"
                     /translation="MVEEILQWLKNISIFLIFMTTVLNLIPEKEDKKPVQFFLGIVMV
                     LIFFQPVLKAGGLEQKMAQTLDRLTLEEEAAAMENVQIRAESFEKQYYVGAWEKETER
                     QIRMYLSESGIPGAAVSVTLEPGNTGEAKEIRIEAPMRQTSIYREEQKKQREMLDEKL
                     TEIKSVLSEVYGTDPAHIMVEISE"
     gene            complement(2116..3279)
                     /locus_tag="PLN77_05535"
     CDS             complement(2116..3279)
                     /locus_tag="PLN77_05535"
                     /inference="COORDINATES: protein motif:HMM:NF021088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HRM43774.1"
                     /translation="MIFCKKAGWGMALLFLCLIFGTGRTQKIEAADTETDQAAAQDEL
                     LAELDFDAIQQEVDSLLSSQQFSFAGTVRSLLQDGDPFRTFRAGNAVLSCAKTAFLAQ
                     KGLMKELFLLVLAGAVLGSFSSLFEGKQVRDASFYMVYLLALALILKNFQSSGENLKA
                     VITSLVAFMRILTPSYYLAAAAAGGASSAVMFYQMLLLVILAVEKLLLALVLPMIHIY
                     LLIALVNDLSGEEILSHMTELLETCVNGLLKSSLGVLVGMQMIRNLIAPALDSLRQMA
                     LWRTAGMIPGIGNAVNAVTELVAGSAVLIRNCFGVTAMLVLLAAGMVPAVQLLVSGLS
                     FRLLAAAAQPVSDKRIAGCLAAAGKGYAMLLRLLLTVEVLFLLTIAILAGTFS"
     gene            complement(3266..3649)
                     /locus_tag="PLN77_05540"
     CDS             complement(3266..3649)
                     /locus_tag="PLN77_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HRM43775.1"
                     /translation="MDILRIAALGTAGVLLGITVKGAKPEFALLVTVGAGLCILAGTV
                     GKMQYLLGMLAQMKSYLPVDSSYLNTLLKMLGITYVGQFSAGICKDAGYSSIAGQIEL
                     FARLAVLAVSMPVLLALLETVHDFL"
     gene            complement(3679..3873)
                     /gene="spoIIIAC"
                     /locus_tag="PLN77_05545"
     CDS             complement(3679..3873)
                     /gene="spoIIIAC"
                     /locus_tag="PLN77_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HRM43776.1"
                     /translation="MEVGILFKIGAVGILVSVLCQVLKHSGREEQAFLASLAGLVLVL
                     FWILPYISALFEDIKQLFTI"
     gene            complement(3901..4416)
                     /locus_tag="PLN77_05550"
     CDS             complement(3901..4416)
                     /locus_tag="PLN77_05550"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HRM43777.1"
                     /translation="MRLAVLLAASLACVGIGFAKSAALSAHVKDLETLQKIIQYLRGE
                     IVTAASPLPDAFWETGRRMRDPYRDFLQDISEDMRRPDGKTLPAILEENTAVHLADTR
                     LTKEEKEELQEAGCSLGTGDRAMQKVNLDRYEARLVQRIAELQKGLPERKRLCQSLGI
                     MGGLFLFLILV"
     gene            complement(4416..5423)
                     /gene="spoIIIAA"
                     /locus_tag="PLN77_05555"
     CDS             complement(4416..5423)
                     /gene="spoIIIAA"
                     /locus_tag="PLN77_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HRM43778.1"
                     /translation="MKRTGRQKDIVTPEKKQEQRELLRLFSANIRKILKNTDLLADDL
                     QEIRLRTGAPLLVVAKDQEYFLTPDGALTGEWEKGYPVSPTDIRDTMDYIGSFSLYAY
                     EDELRQGFLTVEGGHRIGIAGKTVIEGEKVKGISHISCINVRVAHEKKGCADRVMPYL
                     WEDGRFLHTLIVSAPGCGKTTMLRDIIRQISDGESPYPGLTVGVVDERSEIAGCYLGV
                     AQNDVGIRTDVLDCCPKAEGMMMLIRAMSPDVVAVDEIGTGEDIRAIESVVNCGCKLL
                     ATVHGNSMEDMKQKPLLNRLVESHVFERYIVLDAKPHAGSVQAIFDGRGTTLYRREAF
                     L"
     gene            complement(5481..6032)
                     /locus_tag="PLN77_05560"
     CDS             complement(5481..6032)
                     /locus_tag="PLN77_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRM43779.1"
                     /translation="MQETVVYLKKITQPEGEKKELERELSQCGWELFRMGMQEQYGLD
                     GEKITIERNAHGKPYLPGHPQICFNISHSGEYAACAFASIPIGMDLQQHRRAAWERMA
                     ERYFSAEDQKRLAAAENAETLFFRIWTEEESYGKWRGTGLSGSLGTEKKEGFCTHFSP
                     EKGYEGAVWAAEPVKICMKRIEP"
     gene            complement(6115..6621)
                     /locus_tag="PLN77_05565"
     CDS             complement(6115..6621)
                     /locus_tag="PLN77_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRM43780.1"
                     /translation="MSLLQNWRDIAYNQEADKKQLQQFWTNYFLIEKNIYEQLLSNPD
                     EVVKGTVKELAEKYKVDVMTMVGFLDGINDSLVIPNPIEEMEEDTVVTLAYDKELLYK
                     NMVDAKADWLYNLPMWDEIFTEEQKKTLYMEQKKSGTVIKGKKIGRNDPCPCGSGKKY
                     KYCCGKNA"
     gene            complement(6732..7208)
                     /locus_tag="PLN77_05570"
     CDS             complement(6732..7208)
                     /locus_tag="PLN77_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM43781.1"
                     /translation="MDTADRKIVELLQEDARLPLKSIADKVSLTSPTVSARIDRLEKD
                     GVITGYRASVNPEVFDYRIKAFINLEVEPVRKKEFYPYITDCPNVIECNCVTGDYSML
                     MEVVFHTTSELDQFIGQLQHFGRTKTQIVFSTSVEHRGIPLGDTRIPILEQEEEKG"
     gene            complement(7217..8566)
                     /locus_tag="PLN77_05575"
     CDS             complement(7217..8566)
                     /locus_tag="PLN77_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM43782.1"
                     /translation="MDSDIVGSILLQVVLIAVNAVFACAEIAVVSCNDAKLEKMAQEG
                     NKRAAKLFHLTEQPSRFLATIQVVITLSGFLGSAFAAENFSDRIVSALVDAGMKIPEK
                     TLDAIAVVLITLLLSYITLIFGELVPKRIAMQKAEQIALGISGLVSGASKVFAPLVWL
                     LTASTNGILRLLGIDPDEDDDQVTEEEIRMMVDAGSEKGAIDNEEKDMIQNVFEFDDL
                     TIDEICVHRTDVDLLWLEDSMEEWEQLIHESRHSYYPVCGENVDDIIGVLDAKDYFRL
                     RTKDRDHVMKEAIKQPYFVPENIKAATLFQNMKKTGNYFAVVLDEYGGMDGIITVRNL
                     IEQLVGDLNDEAEIGQPSEIELISTDTWKIMGSASLDDVAEELKIKLPVDEYETFGGY
                     IFGELGAVPDDGSQFELETDDLWIKVMRVKEHRVENTVVKKLPPKEKGEQEERKERE"
     gene            complement(8602..>9724)
                     /locus_tag="PLN77_05580"
     CDS             complement(8602..>9724)
                     /locus_tag="PLN77_05580"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM43783.1"
                     /translation="RPLTEGMNILGAHIDSPRLDVKQNPLYEDNGFTYLDTHYYGGIK
                     KWQWVTLPLAIHGVVAKKNGEVVDVVIGEDDNDPVFCVTDLLIHLSREQQTKPANTVV
                     EGENLDLLIGSQPLEGTEKEAVKAMTLKLLNDKYGIEEEDFLSAELEIVPAGKARELG
                     MDASMILAYGQDDRVCAYTSLVAMLEVEAPEKTSCCLLVDKEEIGSVGATGMQSHFFE
                     NTMAELLALAGEGTELALRRCLASSRMLSSDVSAAFDPLYAAAFEKKNAAFFGKGIVI
                     NKFTGSGGKGGSNDANAEYVGALRAIFDDAKVNWQTAELGKVDVGGGGTIAYIMSLYG
                     MEVIDSGVPVLSMHAPWEVTSKADIYEAKKAYVAFLKNA"
BASE COUNT         2075 a         2830 c         2259 g         2560 t
ORIGIN      
        1 aaaaaatccg ggaacgcatg attttgcgtt tccggatttt ttacgtggat atcaaaattt
       61 actgagcact ctcctctgcg gctgtgtcgg cggcggagct catgggggtg atgacgatgt
      121 tttctacgcc gattccggtt ttgcgggtta cgatgtcttc gatctgggca cgctgttcct
      181 cgcttacttc tttcatcggg acaacgatgt cggcggtttc tccggtcagg tttacgacga
      241 cgtcggagaa gccttttgcc tcgagaagaa gttcggtggc agattctttt tctacaagtt
      301 ctgtcatttc caccatactc tggacggcgc tctggcgctc ggtttcggag aggtttgtat
      361 tgttgatgat ttcatttaag gtatctttgt tttgggaacg gatctgttcc cggtcgattc
      421 tggcctgggc catgtatgta gatgccccgg caaggacagc ctctcccggt gtttctgtga
      481 tggtccgcga ggaatcgcca gcggctccgg cgtccacctc atcggtgatg tcggtgtcaa
      541 ggctggagat gtcttttaat acggtgcttg tgtctgcggt ggttccgtct gccgttcctt
      601 tggtggacag ggtgctgtcc gcatagttga tgtatccggc gacggcaatc atgatcgcaa
      661 gggcggtgat gatgacctgg tttttccgga attttttctt tttcccggcc gtctttttcg
      721 gcgatggtgt ctctgccatt ttctcaggtg cattcatttc ctgttctaat ttttccacgc
      781 atttgactcc tttacttcat tttgactact ttaatcttat ttgcgcccac ctgaaataat
      841 gccatcacgg cctcctgaat ctcctgtatc gtcaccgggt ttcccccgcc ttctgcgata
      901 acgagcacgc cgcgcaccgc cgggtactcc tcggaaacca catacggtgt ctcggctccg
      961 ttctgtgtct tttcatagac agttgcctca tcctgcgacg aggatgtaac gcttccggtc
     1021 tcctctccct tcttgttttc ctccgttgtg ctccgcgttg ggacatcttt ttccacaatc
     1081 ttttttcctg tggattccag cgtcaccacg acgtgcactg cgcccacacc gtccatctgg
     1141 gaaagtgcgt cacaaaggcg ctgttccatc tcctcctgca ccgaaagtcc cgcatccccc
     1201 gccggacgcg cagccgtctc ctgcggctgt tctgtctcct caccggtctt ttttgttccc
     1261 gtcggcagca gcaggacaac gccgattgcc gccagcagga agtacagcat ccactgctcc
     1321 tttttctgtt tccggaaaaa cgccgctgcc ttttccctca taggcctatt ccgaaatctc
     1381 taccataata tgcgccggat cggttccgta gacctcactt aaaacacttt tgatctctgt
     1441 cagcttttca tcgagcattt cccgctgttt tttctgttct tcgcggtaaa tgctcgtctg
     1501 ccgcatcggc gcttcaatgc ggatctcttt tgcctcaccg gtgtttcccg gctccagcgt
     1561 taccgaaacg gctgcccccg gaatgccgct ctctgacaga tacatccgga tctggcgctc
     1621 cgtctctttt tcccaggcgc ccacgtaata ctgtttttca aagctttctg cacgaatctg
     1681 tacattttcc attgctgctg cctcctcttc cagcgtcaga cggtcgagcg tctgcgccat
     1741 tttctgctcc aaacctcccg cttttagcac gggctggaaa aaaatcagta ccatcacaat
     1801 tccaagaaaa aactgaaccg gcttcttgtc ctccttctcc gggatcaggt tcagcacagt
     1861 tgtcataaaa atcagaaaaa ttgaaatatt cttaagccac tgcagaatct cttctaccat
     1921 gccgcctccc gccgctgcct gcccaactac acgctagagc gtgtctgaaa aaccatttcc
     1981 gcaagctgca agccccactt tgcggtatat tttaccctca ttcggttgcc gtagcccgct
     2041 acgccgccct cattcggata aaatctccca caaactggga ctcgcagctc acgaaaagcc
     2101 tttttcagac aagctctagg aaaaagttcc cgccaggatt gcaatcgtca gcagaaacag
     2161 gacttctacg gtcagaagga gacgcagaag catcgcatat ccttttcctg ccgccgcaag
     2221 acatcccgcg atccgcttgt ccgagacggg ctgcgccgcc gcggcgagaa ggcggaagga
     2281 aagacccgaa acgagaagct gaacggcggg gaccatgcct gctgcgagca gtaccagcat
     2341 tgccgtcacg ccaaagcagt tgcggatcag caccgcggag cctgccacca actcggttac
     2401 agcattcacc gcatttccga tgccgggaat cattccggca gtccgccaga gtgccatctg
     2461 ccgcagggaa tcgagcgccg gtgcgatcag attccggatc atctgcattc ccacaagtac
     2521 gccgagcgac gattttaaaa ggccgttcac gcaggtttcc agcagctctg tcatatggga
     2581 caaaatctcc tcgccggaaa gatcgtttac aagggcgatg agcagataga tatggatcat
     2641 cgggaggacg agggcgagca gaagcttttc gacagccaga atcactagaa ggagcatctg
     2701 ataaaacatc acggctgatg atgcaccacc ggcggccgcg gccgccagat agtaggatgg
     2761 cgtcaggatc cgcataaaag cgaccagcga tgtgatgaca gctttcaggt tttccccgct
     2821 cgactggaaa tttttcagaa tcagtgcaag cgcaagcaga tagaccatat aaaaactggc
     2881 gtccctgacc tgttttcctt caaagagaga ggaaaaactg ccgagcaccg cgcctgccag
     2941 aacgaggaga aacagctcct tcatcagccc cttctgcgca aggaatgctg ttttggcaca
     3001 agagagaacg gcatttccgg cacggaaggt gcggaaggga tcgccatcct gcaaaagaga
     3061 acgcaccgta ccggcaaaag aaaactgctg gctggacagc aggctgtcca cttcctgctg
     3121 gattgcatcg aagtcgagtt ctgcaagaag ttcgtcctgc gctgctgcct gatccgtttc
     3181 ggtatccgct gcttcgattt tctgcgttct tcccgttccg aaaatgagac acagaaaaag
     3241 cagcgccatg ccccatccgg cttttttaca gaaaatcatg aaccgtttcc agcagagcga
     3301 gcagcactgg catactgacg gcaaggaccg ccaggcgggc gaacagttca atctgccctg
     3361 caatggaaga atagcccgca tccttacaga tcccggcgga aaactgcccc acatacgtga
     3421 tcccaagcat tttcagcagc gtattcagat acgatgagtc caccgggaga tacgatttca
     3481 tttgtgcgag catgccgagc agatactgca tttttccgac ggtcccggca agaatgcaga
     3541 ggccggcacc gaccgtcaca agaagggcaa attccggctt tgctcccttt actgtgattc
     3601 caagcagcac gcccgccgtg ccaagcgcag cgatccgaag tatatccata tgatcccctc
     3661 cgttttttct ccctgctctt aaattgtaaa cagttgtttg atgtcctcaa acagcgcgga
     3721 aatataggga agaatccaga acagcaccag cacaagaccg gcaaggcttg caagaaatgc
     3781 ctgttcttcc cggccgctgt gttttaatac ctggcacagg accgatacca gaatcccgac
     3841 tgccccgatt ttaaatagaa tcccgacctc cacgcctgct cctccgtttt cagacacgcc
     3901 ctaaaccaga atcaaaaata aaaaaaggcc tcccatgatt ccaagactct gacagagcct
     3961 cttcctttcc ggcaatcctt tctgcagttc cgcgatccgc tgcacaaggc gtgcctcata
     4021 ccggtccaga ttcaccttct gcatggcgcg gtccccggtg ccaaggctgc atccggcctc
     4081 ctgcagctct tctttttctt cttttgtaag gcgtgtgtcc gccagatgga ctgccgtatt
     4141 ctcttccaga atcgccggca gtgtctttcc gtccgggcgg cgcatatcct cggaaatatc
     4201 ctgtaaaaaa tcccggtacg gatcccgcat ccgccttccc gtctcccaga acgcatccgg
     4261 cagcggggaa gccgccgtca caatctctcc acgcagatac tggataattt tctgcagtgt
     4321 ttccagatct ttcacatggg ccgacagcgc cgcactcttt gcaaagccga tcccgacgca
     4381 ggcaagagaa gccgccagta aaactgcaag ccgcatcaca gaaacgcctc ccttcggtac
     4441 agtgtcgttc ctcttccgtc aaaaatagcc tgtacgcttc cggcgtgggg tttcgcatcc
     4501 agcacgatgt accgctcgaa aacgtgggat tccacgaggc ggttcaggag aggcttctgc
     4561 ttcatatcct ccatggaatt gccatgcacg gtcgcgagca gcttgcaccc gcagttaacg
     4621 acggactcga tcgcacggat gtcctcgccc gttccgatct catcgactgc caccacatcg
     4681 ggcgacatgg cgcggatcag catcatcatg ccctccgcct tcggacagca gtcgagcaca
     4741 tcggtgcgga tgccgacatc attctgcgca acgccgagat agcagcctgc gatctcgctc
     4801 cgttcatcga ccacaccgac ggtcagcccc ggatacggcg actccccgtc cgaaatctgg
     4861 cggatgatat cgcgcagcat cgtcgttttg ccgcagccgg gtgcggagac aatcagggtg
     4921 tgcaggaaac ggccgtcttc ccaaaggtac ggcatcactc ggtccgcgca gccctttttc
     4981 tcatgagcaa cccgcacgtt gatgcaggat atatggctga tgcctttcac cttttcccct
     5041 tcgattaccg tctttccggc aatgccgatc cggtggccgc cctccacggt cagaaaaccc
     5101 tgccgcagtt catcctcgta ggcgtagagg gaaaaacttc ctatgtaatc catcgtatcg
     5161 cggatatccg tcggtgagac gggataacct ttctcccact ccccggtcag tgctccgtcc
     5221 ggggtcagaa agtactcctg atcctttgcg accaccagaa gcggcgcgcc ggtccgcaga
     5281 cggatttcct gcaggtcatc ggccagcaga tctgtgtttt tcagaatttt ccgtatattc
     5341 gcagaaaaca ggcgcaaaag ttccctttgc tcctgttttt tctctggcgt aacaatatcc
     5401 ttttgtcttc ctgtcctttt catcttcacc acctcttatc gttatatata tgagatggcg
     5461 ggacaggtta tgcacgattt ttatggttct attcttttca tgcagatttt cacgggctcc
     5521 gctgcccaga cggcgccttc atatcctttc tccggactga aatgtgtgca gaagccctct
     5581 ttcttttccg ttccaagaga acccgaaaga ccggttcccc tccacttgcc gtagctctcc
     5641 tcctcggtcc agatccggaa aaacagcgtt tcagcattct ccgctgcagc aaggcgtttc
     5701 tggtcttcgg cggaaaaata ccgttctgcc atgcgctccc aggcagcgcg gcggtgctgc
     5761 tgcagatcca tgccgatcgg aatggaagca aaagcgcagg cagcgtattc ccccgaatga
     5821 gaaatattaa aacagatctg cggatgcccc ggcaggtacg gttttccgtg cgcattccgt
     5881 tcgatcgtta tcttttctcc atccagccca tactgttcct gcattcccat ccggaacagc
     5941 tcccatccgc actgcgagag ttcccgttcc agctcttttt tttcgccctc cggctgtgta
     6001 atttttttca gataaactac cgtctcctgc atcctgcttc tccctcttca tagaaaaaga
     6061 gaccgcgaag cctttcaact ttcggctccc cggtctcttc tcgaaaattc tttcttatgc
     6121 gtttttaccg cagcagtatt tgtatttctt tccgcttccg cacgggcatg gatcgtttct
     6181 tccgattttt ttgcctttga ttacagtgcc ggattttttc tgctccatgt aaagcgtttt
     6241 cttctgctct tctgtgaaga tctcatccca catcggcagg ttgtacaacc agtctgcttt
     6301 tgcgtctacc atgtttttgt acagaagctc tttatcgtaa gccagtgtaa caacggtatc
     6361 ctcttccatc tcctcgatcg ggttcgggat cacaaggctg tcgttgatgc cgtccaggaa
     6421 accaaccatg gtcataacgt ccactttgta tttctctgcc agctctttta cggttccttt
     6481 taccacttca tccgggttgg aaagaagctg ctcgtaaatg tttttctcaa tcaggaaata
     6541 attggtccag aactgctgga gctgtttttt gtctgcttcc tgattatagg cgatatcacg
     6601 ccaattctgt aataaactca tgttattatc catcctttat ctgtttctct gagcgcgttt
     6661 tttcgcgccg tcccctagcg ggattccggt ttttgtaaca cgctttagta tatcggaaag
     6721 tccgcagaaa atcaaccttt ttcttcttcc tgctccagaa tcgggattct ggtatcgccg
     6781 agcggaattc ctctgtgttc caccgatgta gagaacacga tctgcgtctt cgttcttcca
     6841 aaatgctgaa gctggccgat aaactgatcc agctcagatg tcgtgtggaa cacaacctcc
     6901 atcagcatcg aatagtcgcc ggtcacgcag ttgcactcga tcacattcgg gcagtcggtg
     6961 atgtacggat agaattcctt tttccgcacc ggctcaacct ccagattgat gaatgccttg
     7021 atccgatagt cgaaaacctc cgggttcaca ctcgcgcggt agccggtgat caccccgtcc
     7081 ttttccaggc ggtcgattct cgccgaaacc gtcggcgaag tgagacttac cttgtctgca
     7141 atacttttga gcggaagcct tgcatcttcc tgcagcagtt ccacaatttt ccggtctgct
     7201 gtatccagtt cacgtttcat tccctctcct tacgttcctc ctgctctcct ttttctttcg
     7261 gaggcagttt ctttaccacc gtattttcca cgcggtgttc tttgacccgc atgaccttga
     7321 tccagagatc gtcggtctcg agttcaaact ggctgccgtc atccggaacc gcaccgagtt
     7381 cgccgaaaat gtagccgccg aacgtttcat attcatcgac cggcagtttg atcttcagtt
     7441 cctccgccac atcatccaac gaggcacttc ccatgatttt ccaggtgtct gtggaaatca
     7501 gctcaatttc cgatggctgc ccgatctccg cctcatcatt caagtccccg acaagctgct
     7561 cgatcaggtt acgcacggtg ataattccat ccatgccgcc gtactcatcc agaaccacgg
     7621 caaaatagtt gccggtcttt ttcatgttct ggaacagcgt ggcagccttg atgttttcag
     7681 gaacaaaata aggctgtttg attgcttctt tcatgacatg atcgcggtcc ttggtgcgaa
     7741 gccggaaata atccttggcg tccagaacgc cgatgatatc gtccacattt tcaccacaga
     7801 ccgggtaata agaatggcgg ctttcatgga tcagctgctc ccactcctcc atcgagtctt
     7861 ccagccacag aagatctaca tcggttctgt ggacgcagat ttcatcaatg gtcagatcat
     7921 cgaactcaaa tacgttctga atcatatctt tttcttcatt gtcgatcgcg cctttttcgc
     7981 tgccggcatc taccatcatc cggatttctt cctccgtcac ctgatcgtcg tcctcatccg
     8041 gatcgatccc cagaaggcgc aggataccgt ttgtcgatgc agtcagcagc cacacaagcg
     8101 gtgcaaacac tttcgaagca ccgcttacaa gaccggaaat cccgagcgca atctgctcag
     8161 ccttctgcat tgcaatcctt ttcggcacca gttcgccaaa gatcagggtg atataagata
     8221 aaagcagcgt aatcagcaca acggcaatcg catccagtgt cttttccgga atcttcatac
     8281 ccgcgtccac aagagcggaa acgattcgat ccgaaaaatt ttctgctgca aaggcacttc
     8341 caagaaaacc ggacagagtg atgaccacct gtatcgttgc aagaaaacgg gatggctgtt
     8401 ccgtcagatg aaacagcttc gccgctcttt tgtttccctc ctgtgccatt ttttccagtt
     8461 tagcgtcgtt gcaggagacc acagcgatct ccgcgcaggc aaataccgca ttgaccgcta
     8521 tcaatacgac ctgcaaaaga atactgccga ctatgtcact gtccaaacgt ttcaaacctc
     8581 ctcatatctt gcattagatt cttatgcgtt ctttaagaat gctacatagg ctttctttgc
     8641 ctcgtagata tctgctttgc tggttacttc ccacggagca tgcatgctta ataccggtac
     8701 accggagtcg atgacttcca tgccgtacag ggacatgatg taagcgatgg ttccgccgcc
     8761 tccgacatca actttaccaa gctctgcggt ctgccagttt actttggcat catcgaaaat
     8821 agcgcggagt gctccgacat actctgcatt ggcatcgttg gaaccgcctt ttccgccgga
     8881 accggtaaat ttgttgatga cgataccttt tccgaagaag gctgcatttt tcttctcaaa
     8941 tgctgctgca tacagcggat caaatgctgc gctgacatca gaagagagca ttctggagga
     9001 agcaaggcat cttcgaaggg ccagttctgt tccctcgcct gcaagtgcaa gcagctctgc
     9061 catggtgttc tcgaagaaat gagactgcat accggttgcg ccgacgcttc cgatctcctc
     9121 tttgtctacg agcaggcagc aggaggtctt ctccggtgct tctacttcga gcattgcaac
     9181 aagagaggtg tatgcgcata cacggtcatc ctggccgtat gcaaggatca tgctggcatc
     9241 cataccaagc tcgcgtgctt ttccggccgg tacgatctca agctctgctg agaggaaatc
     9301 ctcttcttcg atgccgtatt tgtcgtttaa gagttttaag gtcatggctt tgactgcctc
     9361 tttctcagtt ccttcgagcg gctggcttcc gatcagaagg tcaaggtttt cgccctctac
     9421 aacagtgttt gccggttttg tctgctgctc tctggagagg tggatcagaa ggtctgtcac
     9481 gcagaatacc ggatcgttgt cgtcctcacc gatcacaaca tcaacgactt cgccgttctt
     9541 tttcgctacc acaccgtgga tggcaagtgg aagggttacc cactgccatt ttttgatacc
     9601 gccgtagtag tgggtatcga gatatgtaaa tccgttgtcc tcatacagcg ggttctgttt
     9661 tacatcgaga cgcggggaat cgatgtgtgc gccgaggatg ttcatacctt cggtcagcgg
     9721 tctt
//
LOCUS       DAOMUF010000071         9493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1791_length_9493_cov_3.428163, whole genome shotgun sequence.
ACCESSION   DAOMUF010000071 DAOMUF010000000
VERSION     DAOMUF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1791_length_9493_cov_3.428163"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLN77_05585"
     CDS             complement(<1..986)
                     /locus_tag="PLN77_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM43784.1"
                     /translation="MSKELAKTYDPKGLEDRLYQKWLDKGYFHATVNPNKKPFTIMMP
                     PPNVTGQLHMGHALDNTMQDILCRFKRMEGYEVLWQPGTDHAAIATEVKVTNMLKEQG
                     IDKDEIGREKFLEHCWEWKKEYGGRINSQLRKIGSSADWERERFTMDEGCSKAVEEVF
                     CRLYEKGWIYKGSRIINWCPVCQTSISDAEVEYQEQAGHFWHINYPVVGEEGRFVEIA
                     TTRPETLLGDTAVAVNPEDERYTDIVGKMLKLPLTDREIPVIADAYVDKEFGTGCVKI
                     TPAHDPNDFEVGKRHNLEEINILNDDGTMNNKCGKYAGMDRYEARKAMVEDL"
     misc_binding    complement(1082..1302)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1413..2213)
                     /locus_tag="PLN77_05590"
     CDS             complement(1413..2213)
                     /locus_tag="PLN77_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM43785.1"
                     /translation="MEGFQVTEIGEKIYCILDAGNSSFYVVEGEEKAAVIDTGITAGT
                     KILPVIRELTDKPLLLVITHAHPDHMYHMDEFDEVYMCHDEKKMDPETLVMLTAGKLK
                     PWEEIHDIRTDSVIPLGGTELAVCQTPGHTPGSVVVLETKQNYLFTGDAIGSGCGVWM
                     QVPGGTSLEEYYDGLVGLMAWLVKKGGRMKFFGGHHTQVFESTAHPVYNPLSLGVLAD
                     LIDLVDQVIHGENLGRPSNAKPFSKEQVRYASYGRAEMQYCASRIHRK"
     gene            complement(2286..2726)
                     /locus_tag="PLN77_05595"
     CDS             complement(2286..2726)
                     /locus_tag="PLN77_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HRM43786.1"
                     /translation="MAKEMETLKDEGLQGNETIEEAILGLQKEPTPELLAHVLTVIRR
                     RMKEGGQAILSVEPPSGDDQIRIGTVKSADGKVWWAAFTSFDEELKGAGSVKSTFLVS
                     LEQLIHMASETKEISGVIFNPWNRTIMLDHTLLSIIIGEENAGH"
     gene            complement(2810..3769)
                     /locus_tag="PLN77_05600"
     CDS             complement(2810..3769)
                     /locus_tag="PLN77_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43787.1"
                     /translation="MILTEEKTYIINVTEVDTDAELGLNKKDIMIEYTNLELLHAVLA
                     STMPYGRLSARYRGKRKAELQSRIAMVESVLETRGDQLAKAEQIMYLDTAERSAICHY
                     LGIIYTRLIAQKLYGIDCMVPLNLIEQPGEKKFVKYNGAYRQDLIGYGKQNAWSVWEP
                     VGRSENSQAAFGNGCRAASEIEKINENPLAKSAACMTYYERGYLNAVVKEPERTGDGT
                     LWFLEENYFKAYYQPLFELFADEQPGELYGSSGGFELELTLPWTEEGKRGFRHLQIGT
                     DPVTIALMREGKYDQILKRMENVLDLSKECRFCGEDGIWVGAE"
     gene            complement(3788..4360)
                     /locus_tag="PLN77_05605"
     CDS             complement(3788..4360)
                     /locus_tag="PLN77_05605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRM43788.1"
                     /translation="MEQKDSAERILKQIPYEIVYSARKTLAVQVKADGSVVVRAPKRM
                     AKYKIEAFLKEKQDWIHTHREKALAAAEQKAANRLTEAERKAAVKRAAQMLAEKTAHF
                     AKEMGVTYGRITIREQKTRWGSCSSAGNLNYNWKLVLMPEHVMDYVVIHELAHRKEMN
                     HSARFYAVVAQQMPDYKVWQSWLKEHGREY"
     gene            complement(4518..4655)
                     /locus_tag="PLN77_05610"
     CDS             complement(4518..4655)
                     /locus_tag="PLN77_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43789.1"
                     /translation="MGKLIRLGATALSMIGILVMILCVQAAILFVRLCIRRKQKKIEL
                     K"
     gene            complement(4678..5433)
                     /locus_tag="PLN77_05615"
     CDS             complement(4678..5433)
                     /locus_tag="PLN77_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRM43790.1"
                     /translation="MRKRTRKHRGTAGKMVGGFLAFFCLILLTAGMFWAIQGYVSYQK
                     AVKEKPLAQAVEEARSGAQWASLEQVPDLYKKAVLAVEDHRYYTHHGIDWISMARAVV
                     HDIRVMKPEQGGSTITQQLVKNMYYDQNKTFARKAAEAWTALYVERQLSKEEILELYI
                     NTIYFGDGYYGLKEASRGYFGKEPQDLTDSEAVLLAGIPNAPSAYAPSRHADLALQRE
                     KQVLAAMVKYGYLEQSETDSLLWDAAADPVLAR"
     gene            complement(5724..6284)
                     /locus_tag="PLN77_05620"
     CDS             complement(5724..6284)
                     /locus_tag="PLN77_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRM43791.1"
                     /translation="MKKSKLTTKQIVLTGMMTAVLAVLSIVTIPLPTGVPVTLQTFAM
                     AFCGYVLGWKLGGASSALYLILGAIGVPVYAGMSGGFGALLGPAGGFIFGFIPMAMLC
                     GAGIKKGFPGKPFVLGIAGLAVCHAFGVVQFSIVTGNTIPASFLAVSVPYIVKDVISV
                     VVAEILGVAVRKALSAAHVMNYGTAA"
     gene            6563..7936
                     /locus_tag="PLN77_05625"
     CDS             6563..7936
                     /locus_tag="PLN77_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM43792.1"
                     /translation="MKPSENPLGSEPVSSLLRRFAIPSVIAMLVSALYNMVDQLFIGH
                     SIGVLGNAATNVAFPLSMVCTSIGLLCGIGGAANFNLCMGRKDPEHAKSYVGNAISML
                     AILGVILCVAVQLFLRPMMLLFGATPDVIDYACTYTRITSIGFPFLIVTIGGSNLIRA
                     DGSPKFSMLCNLIGAIVNTILDPLFIFVFHMGMAGAALATITGQILSFALVVFYLRGF
                     KTLPLSLSDLKPKIACWARIAALGATPAFNQVAMMVVQIVMNNTLTYYGSNSVYGSDI
                     PLACAGIISKVNMLFFSFVIGISQGLQPIVSFNFGAQKYDRVKDAYKKAVFAATAISI
                     VAFLCFQFFPRQIIGIFGSGSEEYLHFAERYFRIFLFFTFLNGIQPVSSNFFTSIGAP
                     KKGIFLSLTRQIIFLLPLLLIFPYLFGIDGVMYTAPIADLAAASVSIVMVVREFKIMA
                     ELQKAAA"
     gene            complement(8137..>9493)
                     /locus_tag="PLN77_05630"
     CDS             complement(8137..>9493)
                     /locus_tag="PLN77_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM43793.1"
                     /translation="YGAPQGKLGKPAKELGAYRKTRLLQPGETQRVVLSFTVEEMASF
                     DDLGKVAKSAYVLEAGSYVFYVGNNVRDAKKLDFTYDLAEAKVTAQYTSLAAPHKLEK
                     RLLADGTYEALPTDNGPVEEEGLERQDKLTLEGFLPAVKAQERKSFGELMEAAKTNPN
                     LMNVVEGKETLDEFVDKLPTEALIHLLGGQPNTGVANTFGMGNLPEYGIPNIMTADGP
                     AGLRIQPQCGVNTTAWPCATLLACTWDPELIEEIGKAGGEEVKENNIGIWLTPAVNIH
                     RSPLCGRNFEYYSEDPLVAGKSGAAMVRGMQSEHIGASVKHFCCNNKETNRKDSDSRV
                     SERALREIYLKAFEIIVKEADPYTIMSSYNLINGVQASENKDLLTGILRGEWDFKGMV
                     TTDWWTHGEHYRETKAGNDIKMANGYEERVQEAFEKGYITRDEIALCAKRILTMILRM
                     D"
BASE COUNT         1912 a         2690 c         2103 g         2788 t
ORIGIN      
        1 tcgagatcct ctaccatggc ttttcttgcc tcgtagcggt ccatgccggc gtatttgccg
       61 cacttgttgt tcatggttcc gtcatcgttt aagatgttga tctcttcgag gttgtggcgt
      121 tttcctacct caaaatcgtt cgggtcgtgt gccggtgtaa ttttaacgca tccggttccg
      181 aactctttgt ctacgtaagc atctgcgata accgggattt cacggtcggt cagcggcagt
      241 ttcagcattt ttcctacaat atcggtgtat ctttcatcct ccggattgac agctacggca
      301 gtatcgccga gcagagtctc cggacgggtc gttgcgattt ctacgaagcg gccttcttct
      361 ccaactaccg ggtagttgat gtgccagaag tggcctgcct gctcctgata ttcaacctct
      421 gcatcggaga tggaagtctg gcataccggg caccagttga tgatgcggct tcctttgtag
      481 atccatcctt tttcatacag acggcagaat acttcttcta cggctttgga gcatccctcg
      541 tccatggtga agcgttctct ctcccagtct gcggaggagc cgatctttct taactggctg
      601 ttgatgcgtc cgccgtactc tttcttccac tcccagcagt gctccaggaa tttttcgcgt
      661 ccgatttcat ctttgtcgat gccctgctct ttgagcatat tggtaacctt tacctcggtt
      721 gcaatcgccg catggtcggt tcccggctgc cagaggactt catatccctc catccgtttg
      781 aaacggcaga gaatatcctg catggtgttg tcgagtgcat ggcccatgtg aagctgtccg
      841 gttacgttcg gaggcggcat cataatggta aacggttttt tgttcgggtt tacagtggcg
      901 tggaaatatc ctttatccag ccatttctga tacagtctgt cctcgagtcc tttcggatcg
      961 taggtctttg cgagttcttt gctcatgaga tcctcctttt atgtttggtg tattgtggta
     1021 tttattcccg cagaggcttg tcttaaatca aaaacgccct tcacatacac ataacatgca
     1081 tgcaaagggc gaaaattccg cggtaccacc ttaatttgta tctcgtttcg gagtctgtaa
     1141 cgtgactcat gcgtgagaac ctaatcatca catcttctgt gcttttcagc tctccggttc
     1201 agaagctacc ttccatgacc accttcccgg acagcctttc agccggtgaa ccatcctctc
     1261 ttttggggcc tgccatgtac tcctcttcct cataaccttt tctttttctg ttggaataga
     1321 aactgcattt atcataatcg cgttttttta ttttgtcaag ccgccgtggg caaaaacctt
     1381 tcttcttatg gaagttttaa gatttcacgc gcttattttc ggtgaatccg cgatgcacag
     1441 tactgcattt ctgcgcggcc gtacgacgcg tagcggacct gttctttcga aaacggcttc
     1501 gcattgcttg gccgtcccag gttttcgccg tggatcacct gatccacaag gtcgatgaga
     1561 tctgcgagaa caccgaggct gagcggattg taaaccggat gcgccgtcga ttcaaacacc
     1621 tgcgtgtgat gaccgccgaa gaatttcatg cggccgccct ttttcaccag ccacgccatc
     1681 agaccaacca gaccatcgta atactcttcc aggctcgttc cgcccggcac ctgcatccag
     1741 acaccgcagc cgcttccgat cgcatctccg gtgaacaggt aattctgttt cgtctccagc
     1801 acaacaacgc tgcccggcgt atggcccggt gtctgacaga cggccagctc tgtgccgccg
     1861 agcggaatca cggaatcggt acggatgtca tgaatctcct cccacggttt taattttccc
     1921 gctgtcagca tcacaagcgt ctctggatcc atcttctttt catcgtggca catgtacacc
     1981 tcatcaaact catccatgtg atacatgtga tccggatgcg catgcgtgat gaccagcaga
     2041 agcggcttgt ccgtcagctc acggatcacc ggcagaatct ttgtgcctgc cgtaatgccg
     2101 gtatcaatga cagccgcttt ttcttcacct tctactacat agaacgatga atttcctgcg
     2161 tccaaaatac agtaaatttt ttccccgatt tctgttacct gaaaaccctc catgatgtaa
     2221 aacctcctta aaataataat gtaccctcgg agtctttctg tgcttgcttt tttcctttcg
     2281 gcaattcagt gccccgcatt ctcttccccg atgataatcg aaagcagcgt atgatccagc
     2341 atgatcgtgc ggttccacgg attgaaaatc acaccgctga tctcctttgt ttcggatgcc
     2401 atgtggatca gctgttccag gctcacgaga aacgtggatt tcacgctgcc ggcgcccttt
     2461 aattcctcat caaagctcgt aaacgcggcc caccagactt ttccgtctgc gctctttacc
     2521 gttccgatcc ggatctgatc gtcgccggat ggcggttcca cggaaagaat cgcctgtccg
     2581 ccctctttca ttctgcggcg gattaccgtc agcacatgtg ccagcagttc cggcgtcggc
     2641 tccttttgca gtcccagaat tgcttcctct atcgtttcat tcccctgcag cccttcgtcc
     2701 tttagagttt ccatttcttt tgccattgct cttgtccttt ctttttcgct tttcccacac
     2761 agaatctgtg taccatcatc cccggcagct tcggcacttc cgatgacatt tactctgcgc
     2821 ctacccagat gccatcttcc ccgcagaatc tgcactcttt tgataaatcc aaaacgtttt
     2881 ccatccgctt taagatctga tcgtacttcc cttcccgcat caatgcaatc gtcaccggat
     2941 ccgttccgat ctgcagatgg cggaagccgc gttttccttc ctccgtccac ggcagagtca
     3001 gctccagttc aaagccgccg ctgcttccgt acagctcacc cggctgttcg tccgcaaaca
     3061 gctcgaaaag cggctgataa tatgctttga aataattctc ctccagaaac caaagtgtcc
     3121 cgtccccggt ccgctccggc tcttttacaa cggcattcag atagcctctc tcgtaatacg
     3181 tcatgcacgc cgcgctcttc gccagcggat tttcattgat cttctcgatc tccgacgccg
     3241 cccggcatcc gtttccaaag gccgcctggc tgttctcgct cctgccgacc ggctcccaga
     3301 cgctccatgc gttctgcttt ccgtatccga tcagatcctg gcggtatgca ccgttgtatt
     3361 ttacaaattt tttctccccc ggctgctcga tcaggttcag cggtaccata cagtcaattc
     3421 cgtacagctt ctgcgcaatc agccgcgtgt agatgatgcc gagataatga cagattgcac
     3481 tgcgctccgc cgtgtcgaga tacataatct gctccgcttt tgccagctgg tcgccgcgtg
     3541 tttcgagcac actctccacc atcgcaatcc gtgactgcag ctccgccttc cgttttccgc
     3601 ggtagcgcgc cgacagacgg ccgtacggca tcgtgcttgc aagaaccgcg tgcagcagct
     3661 ccagattcgt gtattcgatc ataatatctt tcttatttaa accgagctct gcgtcggtat
     3721 cgacttccgt cacattgatg atatatgttt tttcttcggt cagtatcatt tgttttgcct
     3781 ctctttttca gtactctctt ccatgctcct tcagccagct ctgccacact ttgtaatcgg
     3841 gcatctgctg tgccaccacc gcgtaaaacc gcgcggaatg gttcatctct ttccggtgcg
     3901 ccagctcgtg gataacgacg taatccatca cgtgctccgg catcagcacc agcttccagt
     3961 tgtagttcag gtttccggcg ctgctgcagc tgccccagcg ggttttctgc tcacgaattg
     4021 tgattctgcc atacgttacg cccatctctt ttgcaaaatg tgccgtcttt tccgccagca
     4081 tctgcgccgc acgtttaacc gccgcttttc gttctgcttc cgtcagacgg ttcgctgctt
     4141 tctgctctgc ggccgccaac gctttttccc ggtgtgtgtg aatccaatcc tgtttttctt
     4201 ttaagaatgc ttcgattttg tattttgcca tccgtttcgg tgcgcgcacc accacgcttc
     4261 catccgcttt gacctggacg gcgagcgttt tacgggcgga atacacgatc tcatagggaa
     4321 tctgttttaa aatccgttct gcgctgtctt tctgttccat ttatttttcc tcaaaagctg
     4381 tctgttttac catttttttg cctgtttttc attatacctt tttcccggcg gaaacgcaat
     4441 cggattgctc acagaaaaaa aacgcgagca gaatcaccgt ttttcttccg gcaattctgc
     4501 tcacattttt ctttctctta tttcagctca atttttttct gctttcttcg gatgcacagc
     4561 ctcacaaata aaatcgccgc ctgcacacag agaatcatca ccaaaattcc aatcatggac
     4621 agcgctgttg cccccagtct gatcagtttt cccattcttt tttcctcact tttctttcta
     4681 tcgtgccaga accggatcgg ctgccgcatc ccacagcagg ctgtccgtct cactttgttc
     4741 cagatatccg tattttacca ttgccgccag cacctgcttt tcccgctgca gcgccagatc
     4801 tgcgtggcgc gacggtgcgt aggcggacgg tgcattcggg attccggcga ggagaacggc
     4861 ttcgctgtcg gtcagatcct gcggctcttt tccgaaataa ccacgcgatg cttctttgag
     4921 tccgtaatag ccatcaccga aatagatggt attgatgtac aattccagaa tttcttcttt
     4981 cgagagctgc cgttccacat agagtgccgt ccacgcctct gccgctttcc tggcaaatgt
     5041 cttattctga tcgtaataca tgttttttac cagctgctgt gtgatggtac tgcctccctg
     5101 ctccggtttc atcacgcgga tatcatgtac aacggcccgc gccatgctga tccagtctat
     5161 cccgtgatgt gtgtaatacc gatgatcctc aacagcaagc accgcttttt tgtacagatc
     5221 cggcacctgt tcaagagaag cccactgtgc tccgcttctg gcttcctcta ctgcctgcgc
     5281 gagcggcttt tccttcactg ccttctgata ggaaacatat ccctggattg cccagaacat
     5341 tccggccgtc agcaggatca ggcagaaaaa tgccagaaaa cctccaacca tttttcccgc
     5401 tgttccccgg tgttttcgtg ttctttttct catggtttca aacccctttg ttgttctttc
     5461 ctgctttcta tcctaccata tctcccttaa cgccttcttg cctctttctt aggaattcat
     5521 taaaattttg aaactgtatg aaatgcattt tgcacgtttt caaataaccg ctgctgctgc
     5581 ggaatacttt aaccgaatca ggcaagtagc gaagcggatt gcgaatatcc gcttgacaaa
     5641 acagaaaaag acagacacct ggcagattgt caggatgcct gtctcttgtt tttatctttt
     5701 ggtttttcag aaacgctctg tgattatgct gcggttccgt agttcattac atgagctgct
     5761 gacagtgctt ttcgaacggc tacaccgaga atttcagcta ctacaacgga aatcacatct
     5821 tttacgatgt acggaacgga aactgccagg aaggaagccg ggatcgtgtt tcctgttaca
     5881 atagaaaact gtaccacacc gaatgcgtga cataccgcaa gacctgcgat tccaagaacg
     5941 aatggtttgc ccgggaagcc ttttttaatt ccggcaccgc agagcattgc catcggaata
     6001 aatccgaaaa taaagccgcc ggccggtccg agaagagcgc cgaagccacc gctcatgccg
     6061 gcataaaccg gaacgccgat cgcgccgagg atcagataaa gagcggacga tgcacctccc
     6121 aatttccagc caagcacgta gccgcaaaat gccattgcaa aggtctgcag cgtcaccgga
     6181 acgccggtcg gaagcggaat cgtcacaatg gacagtaccg caagcactgc cgtcatcatg
     6241 ccggtcagta caatctgttt tgtggtaagt tttgattttt tcatgaatgt aagtcctcct
     6301 cgcaggttct tgtatgcaat tctttctgtc tttgaattgt taaccttgtt acaatatagg
     6361 ttaatatttc atttttgtca atctcttttt taaaaatgca gagagttctg ctacgattct
     6421 gttacacttc ctgaaaatag cggtcaccct cttgttttct gttttctttc tatggtatac
     6481 tatatagtct cggagttttt ccgggaattt tcacccgata caaacgatga acttttacct
     6541 catttgaaag gagataccct ctatgaaacc ttccgaaaat cctctcggct cggaaccggt
     6601 cagctctctt ttgcggcgtt ttgcgattcc gagcgtcatc gcgatgctcg tcagcgccct
     6661 ctataacatg gtagaccagc tctttatcgg tcacagcatc ggcgtgctcg gaaacgcagc
     6721 gaccaacgta gcgttcccac tcagcatggt ctgcacatcc atcggtctgc tctgcggcat
     6781 cggcggtgct gcaaatttca atctctgcat gggcagaaag gatccggagc acgcgaaatc
     6841 ctatgttgga aatgccatct ccatgctggc aatcctcggt gtgatcctct gcgttgccgt
     6901 tcagcttttt ctgcgtccga tgatgcttct ctttggtgca acgccggatg tcattgatta
     6961 cgcctgtact tacaccagaa ttacctccat cggcttcccg tttctgattg tcacgatcgg
     7021 cggctccaac ctgatccgcg cggatggcag cccgaaattt tccatgctgt gtaacctgat
     7081 cggcgcgatc gtcaacacga ttctcgaccc gctgttcatc ttcgttttcc acatgggcat
     7141 ggcaggtgca gcgctcgcca cgatcaccgg acagatttta tcgttcgcgc tcgttgtctt
     7201 ctatctgcgc ggttttaaaa cgctgccgct ttctctttcc gatctgaagc cgaaaatagc
     7261 ctgctgggca cggatcgctg cgcttggtgc aactccggca ttcaatcagg tggcaatgat
     7321 ggttgttcag atcgttatga acaacacact gacctactac ggctccaatt ccgtctatgg
     7381 aagcgacatt ccgcttgcat gcgccggcat tatttcaaag gtgaatatgc tgttcttctc
     7441 ctttgtcatc ggcatttccc agggacttca gccgattgtc agcttcaact tcggtgcgca
     7501 gaaatatgac cgcgtgaagg acgcctataa aaaagcagtt tttgcggcga ctgccatttc
     7561 catcgtcgcc ttcctctgct tccagttctt tccgcgccag attatcggca tttttggttc
     7621 cggttcagaa gagtacctgc attttgcaga gcggtacttc cgcattttcc tgtttttcac
     7681 attcctgaac ggtatccagc cggtcagctc aaacttcttt acctccatcg gagcgccgaa
     7741 gaagggtatt tttctttcgc tgacgagaca gattattttc ctgcttccgc tgctgctgat
     7801 ttttccgtat ctcttcggca ttgacggcgt gatgtacact gcaccgattg cagaccttgc
     7861 agccgcatca gtcagcatcg ttatggtcgt gcgggaattt aagatcatgg cagagttaca
     7921 gaaggctgcc gcctgaaaac agtttttttc ctgtagtttc cgctcgtttt tactgcgctg
     7981 cggtcgccag agtcttgcta cagatcattg ccgtgtaaac aggaaagaat cctgtccgga
     8041 aagtaaaaaa tcgtatgctg ttgctttttc cacaacagca tacgattttt ttatgtgtta
     8101 tccacacttt tctttattta ttcacatgca tccacatcag tccatgcgaa gaatcatggt
     8161 caggattcgt tttgcacaaa gagcgatttc gtcgcgggtg atgtatccct tctcaaatgc
     8221 ctcctgtacg cgctcctcgt atccgtttgc catcttgata tcatttccgg cttttgtctc
     8281 gcggtaatgc tcgccgtgtg tccaccagtc ggtcgttacc attcccttaa agtcccactc
     8341 accgcgcaga ataccggtca aaagatcctt gttctcagat gcctgaacgc cgttgatcag
     8401 gttgtaggac gacatgattg tgtatggatc tgcctctttt acgatgatct cgaaggcttt
     8461 caggtaaatc tcacgcagcg cacgctcgga aacgcgggaa tcgctgtctt ttcggtttgt
     8521 ttctttgttg ttgcagcaga agtgtttcac acttgccccg atatgctcgg actgcatacc
     8581 gcgtaccatt gccgcaccgg attttccggc cacaagcgga tcctcggaat aatactcgaa
     8641 gtttcttccg cagagcgggc ttctgtgaat gttgacagcc ggggtcagcc agatgccgat
     8701 attgttttct ttgacttcct ctccgccggc tttaccgatc tcttcgatca gctccggatc
     8761 ccaggtgcag gcaagcaatg ttgcacacgg ccatgcggtc gtgttgacgc cacactgcgg
     8821 ctgaatgcga aggcctgccg gtccgtctgc ggtcatgata ttcgggatgc cgtactcagg
     8881 cagatttccc atgccaaacg tattggcaac tcctgtgttc ggctgaccgc cgagcagatg
     8941 aatgagtgct tctgtcggaa gcttatctac aaactcgtcg agcgtttctt ttccttcgac
     9001 cacattcatc aggttcggat tcgtctttgc tgcctccata agctcgccaa agctctttct
     9061 ctcctgtgct ttgactgccg ggaggaagcc ttccagagtc agcttatcct gacgctccag
     9121 tccttcttcc tcgacagggc cgttatcggt cggaagcgct tcgtaggttc catccgcaag
     9181 gagacgtttt tccagcttat gcggagcggc aaggctggtg tactgtgcag tcacttttgc
     9241 ctctgccaga tcgtaggtga agtccagttt cttcgcatct cttacattgt ttccaacgta
     9301 gaagacgtag cttcctgcct caagaacata ggcagatttt gcaacctttc caaggtcatc
     9361 aaaagatgcc atttcttcca ctgtaaagga aagaacgact ctctgtgtct cgcctggctg
     9421 cagcagtctt gttttccggt aagcgccgag ctctttggcc ggtttgccaa gcttgccctg
     9481 cggtgcgcca tag
//
LOCUS       DAOMUF010000072         9465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1802_length_9465_cov_4.921467, whole genome shotgun sequence.
ACCESSION   DAOMUF010000072 DAOMUF010000000
VERSION     DAOMUF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9465
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1802_length_9465_cov_4.921467"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLN77_05635"
     CDS             <1..585
                     /locus_tag="PLN77_05635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM43794.1"
                     /translation="NRWDKLLQIHTMGRDDSRSDLYRYPYEPTPYCVLERMANTGMIR
                     KGNTLLDYGCGKGRVDFFLSAQTRCQSIGVEYDDRIYAKAMENKKAAASGARTEFVLE
                     SAENFPVPAEVDRVYFFNPFSLEILRKAMARLLESYYENPREIQLFFYYPSDAYISYL
                     MTVPELEFLDEIDCMDLFPGGDSRERVVIFTIQE"
     gene            658..1074
                     /locus_tag="PLN77_05640"
     CDS             658..1074
                     /locus_tag="PLN77_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43795.1"
                     /translation="MKYQVMRIEEDLDFGCEERGADEPVMAVVVLKDENGTESRIRQE
                     DQMLYDWNIEEGDTVILNENGELRKVLSGDWTQNCDSKNVDTTKFVNMMQAVKAGKQI
                     DWICPFCSGTVERLEQKGGKTVIGCTSCDMRIQIEN"
     gene            1116..1526
                     /locus_tag="PLN77_05645"
     CDS             1116..1526
                     /locus_tag="PLN77_05645"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRM43796.1"
                     /translation="MVKPIMRDVLFLGQKSVPATEEDLQVGRDLQDTLAANREACVGM
                     AANMIGVKKRIIIVNAGFRDIIMFNPVIVRKSGPYETEEGCLSLPGVRKTKRYQNIEI
                     EYYDWNWKKQRQKFTGWPAQICQHEMDHLEGILI"
     gene            1570..1788
                     /locus_tag="PLN77_05650"
     CDS             1570..1788
                     /locus_tag="PLN77_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43797.1"
                     /translation="MVKGIIYNPNVEDPAKEQSGYMERLWEFNQLKPSEGDKKVKYMK
                     ETFAEVTIGEHDREYFYRDEKIDGENLK"
     regulatory      1893..2067
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2253..3731
                     /locus_tag="PLN77_05655"
     CDS             2253..3731
                     /locus_tag="PLN77_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HRM43798.1"
                     /translation="MIAFLNWLDGVLWGVPLIALMILTGLYFTVRSGFFQFRHFGWIM
                     KRTGGKLFQKEDKASEGKGMLSSFEAISTAIGGTVGFGNIAGVATAVAAGGPGAIMWM
                     WLSSLLGMILKQVEVTLGCYYRHTNEKGEYYGGPTYYMECGLGEERHWGKLWLIPALI
                     FGIGIFSTFFVTSSNLTASQVVAGAFGIENINLGSFKVEGVIVMGVLLCILTYVVTSG
                     GTKKIASLFSKLVPFMSVLYILMGIGMIIININRVPGVFAAIVTNAFTGTAAIGGFAG
                     CAVSEIIRVGMARSVYSNEAGWGTSPMIHASAKTPHPVEQGLWGSFEVFFDTMVICTI
                     TALSVILSGNWTDGTNGGTLALSAFASGFGKFGSILLAVIMVIFTVTTSGGWFTYYLA
                     ILEHLYKKEGKMKDIVMKIFYAVRALPGVLWTIYLVKTNNQGFIWTVVDITSAIPTFV
                     NVAVILILSNQYFKLLKDYKARNFGQGTVDKNACLFYEEKTK"
     gene            3849..5465
                     /locus_tag="PLN77_05660"
     CDS             3849..5465
                     /locus_tag="PLN77_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRM43799.1"
                     /translation="MKTIYKNGAVYTGKLPLVEAFAVEDGKFVFAGSSAEADALAAAG
                     DKVVDLGGKFVCSGFNDSHMHLLNYGSSLLKAQLAAHTESLEDMLACLDTFQKEHPRK
                     DGAWLMGRGWNQDYFTDVDRMPNRYDLDRVSTEVPICATRACGHCLVVNSKALELLGV
                     TADTPQPEGGEIGMENGEPDGRFFDNAMEPVYDSIPVPSKEEIKDMIRSACKALNSYG
                     VTSSQSDDYCVYRAVPWQTVNEAYKELEESGELTVRVYEQSNFTDLESLKGFVEAGNV
                     TGIGTDRFKIGPLKMLGDGALGARTAFLSRPYADDPSTCGIPVFTQETMDEMIGYANA
                     HGMQAAVHTIGDACLDRVLSAYEKALAENPREDHRHGIVHCQITRADQLEKIAKMHLH
                     VYAQSIFLDYDNHIVEERVGKELASTSYSWKTLMNNGVSVSNGTDCPVELPDALACMQ
                     CAVTRTTLRDHVGPYLPDQKFTVQEALDSYTIRSAEASFEENVKGQIAEGMMADFVVL
                     GQNPFEVDESTIKDIPVCRTYLAGELVYEA"
     gene            5726..6364
                     /locus_tag="PLN77_05665"
     CDS             5726..6364
                     /locus_tag="PLN77_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRM43800.1"
                     /translation="MLGRTHFFIGTAAALAVLQPQTVPALVAGAGAAAIGGLISDIDV
                     GTSQAHRDADKIITATVAVAVLTILAEYKLNLGIYRRLTSDSSVLRLLAGTAAFLLIC
                     AYGKQQPHRSFMHSFAALALLTACVDIIYPDVSAYFAVGFLSHLVLDFFNRKPEKLFW
                     PWKKGFCLGLCSARGLVNRALLGCGMVSLAVILVISAPAGRLMAKIMRAIYG"
     gene            6620..7879
                     /locus_tag="PLN77_05670"
     CDS             6620..7879
                     /locus_tag="PLN77_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRM43801.1"
                     /translation="MNEEMKNQTEQLPLLKVHMMGSFFLEYGDQPISFRKTMATKSMR
                     LLQIFLYNKDNGISRGRLLESTYGREDVADAANSLRVGVHRLKRSLVEAGLPQFEYIY
                     TQKGVYHWTSPMPVEVDAIDIKNKILEAGKETDEKARMDQMIEALELYTGEFLADFGE
                     EEWVQAERAQLKKVYSDALKEVSEYLLKNEEFDELQKLTSVASELYPFDEWQAVQMQA
                     LIGLERYKEAMKLYEQTSKHYFEELGVTPSEKLVEQYRYLGSRMGSRHRVIEEVQADL
                     QESPGEKGGAFFCSLAGFRDCYRLVYRMSELNGQMPWLMLCTITDGKGYPAKGGPRLD
                     RMSEKLLEVMKRSLRHSDFFAKYSPSQYVILLQGGSQRGCELVYKRISERFSEEKKGW
                     AKNLKYSALPAIQTEKELEFLEGDDVL"
     gene            7876..8226
                     /locus_tag="PLN77_05675"
     CDS             7876..8226
                     /locus_tag="PLN77_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRM43802.1"
                     /translation="MIRVQLLGRFRMYDETRCLNEEAIHSPMITKLLVYMICNKNHIV
                     TLEQLTEALWTDEESENPYGALKNMMYRLRGTLRKVWPVERGSCLLYEGCHGTDEKPG
                     APQTAKEKYRFSVI"
     gene            8394..>9465
                     /locus_tag="PLN77_05680"
     CDS             8394..>9465
                     /locus_tag="PLN77_05680"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRM43803.1"
                     /translation="MSYREVYEKWCNDPYFDEASREELKALEGNEAEIEDRFYRQLEF
                     GTGGLRGVIGAGTNRMNIYTVRQATQGLANYIISQNGQEKGVAIAHDSRRMSPEFARE
                     AALCLNANGIKTYLFESLRPTPELSFAVREFGCISGIVITASHNPREYNGYKVYWEDG
                     AQITPPHDKNILAEVAKVTEFSAVKTMEEDDARAEGLFQVIGTDFDDRYVAKLKEQSI
                     HPEIIKDAAKDMKIVYTPLHGTGNVPVRRVLRELGFNQVYVVKEQKVPDGNFPTVAYP
                     NPEDEKAWTLALKLAKEVDADIILATDPDADRLGVYAKDSKTGEYVSFTGNMSGMLIA
                     EYILRERTKNGTMPENPALVETI"
BASE COUNT         2723 a         2159 c         2569 g         2014 t
ORIGIN      
        1 aaccgctggg acaagctgct gcagatccac acaatggggc gggatgacag ccggtcggat
       61 ctgtaccggt atccgtatga gccgaccccg tactgcgtgc tggagcggat ggcaaacacg
      121 gggatgatcc gaaagggaaa tacgctgctc gattacggat gcggaaaggg ccgcgtagat
      181 ttcttcttat cggcgcagac ccggtgccag tccatcggtg tggagtatga tgaccggatt
      241 tatgcgaagg caatggaaaa caaaaaggcg gcggcctctg gtgcgcggac ggagtttgtg
      301 ctggagagcg ccgaaaattt tcccgttcct gcggaagtgg accgggtgta tttttttaac
      361 ccgttttcgc ttgaaatctt aagaaaagcg atggcaagac tgctggaatc gtactacgaa
      421 aatccgcggg agatccagct gtttttctat tacccgtcgg atgcttatat ctcgtatctg
      481 atgacggtgc cggaactgga atttctcgat gagatcgact gtatggatct gtttccgggc
      541 ggggacagcc gggagcgggt tgtgattttt acgattcagg aataaaaagt tttgtaacag
      601 caaaaaccgt gtggaataca aaaaagcatg aattatattt gaagaaaggg cgtttttatg
      661 aaatatcagg taatgcgaat tgaggaagac cttgatttcg gctgtgagga gcgcggtgcc
      721 gacgagcctg tcatggcagt tgttgttttg aaagatgaaa acggaacaga atcgcgcatc
      781 cgccaggagg atcagatgct gtatgactgg aacatcgagg agggcgacac cgttattctg
      841 aatgaaaacg gcgaattgcg aaaagtactc tccggtgatt ggacgcagaa ctgtgattcg
      901 aaaaatgtag atacgacaaa attcgtcaac atgatgcagg ccgtcaaagc cggaaaacag
      961 attgactgga tctgtccgtt ctgcagcgga acagtagaac gattggagca gaaaggcgga
     1021 aaaaccgtaa tcggatgcac atcgtgcgat atgcggattc aaatagaaaa ttaaacgatt
     1081 ttggccttta aggccgaaag agaaaggaaa cacaaatggt aaaaccaatt atgagagacg
     1141 ttctcttcct cggacagaaa tccgtcccgg cgacagagga agatctgcag gtgggaagag
     1201 atctgcagga cacactggca gcaaaccgcg aggcatgcgt cggcatggcg gcaaatatga
     1261 tcggcgtgaa aaaacgcatc atcatcgtaa acgccggatt ccgcgacatc atcatgttca
     1321 acccggtgat cgtccgcaaa agcggcccct acgaaacaga agaaggttgc ctctccctgc
     1381 caggcgtgcg gaaaacaaag cggtaccaga acatcgaaat cgaatactac gactggaact
     1441 ggaaaaaaca acgccagaaa ttcaccggct ggccagccca gatctgccag catgagatgg
     1501 atcatctgga agggatactt atttagatcc tacagcagac aggaggacgg tatgacagac
     1561 agagaatgaa tggtaaaagg aatcatttac aacccgaatg tggaagatcc ggccaaagag
     1621 cagtccggct atatggagcg tctgtgggag ttcaaccagc tgaagccgtc agagggagac
     1681 aagaaagtca aatatatgaa ggaaactttc gcagaagtca ccatcggcga acacgaccgc
     1741 gagtattttt accgggacga aaaaatcgac ggggagaatc tcaaataaat cgggacaaat
     1801 ttcgaaaata taaaattttt gtaaaataat gtcgaaaaaa cgattgacaa gtggcgaaaa
     1861 gaaacatatg ataatacctg tcaatataca tattcggcaa tagaggcgcg aaggctaaga
     1921 gtagctgtcc tgagacaacg gggtctggga cgggcaggga aaggagcatt cgccgaaacg
     1981 gggcagttcc cgaaaaactg tctgcgttgg ggtacagtcg aacaggctgt gcactctcac
     2041 cgtactggtg gaggagctat tgcttcatga acgtagattt aatggagcag tggtgaagac
     2101 cgctgctctt tttattgtac tgcagttgga accgtgacaa aaggttccgg gggtggaaat
     2161 ctgtttggtt ggagaacgaa agacgaaacg ggtgttacag gatacgtctg aagaaaactg
     2221 ttgccggaaa agaaaaaagg gaggattttg acatgatagc atttcttaac tggctggacg
     2281 gtgtcctttg gggcgtgccg ctgatcgcgc tgatgattct gaccggtctg tactttaccg
     2341 ttcgttctgg atttttccag ttccgtcact ttggctggat tatgaagaga accggcggaa
     2401 aactgttcca gaaagaagac aaagcaagtg aaggaaaggg tatgctttcc tcgtttgagg
     2461 cgatttcgac cgctatcggc ggtaccgtag gattcggtaa tatcgccggt gttgcaactg
     2521 cagttgcagc aggcggcccg ggagccatta tgtggatgtg gctttcgtct ctgcttggta
     2581 tgatattaaa acaggttgag gttaccctcg gctgctacta ccgtcacacc aacgaaaaag
     2641 gtgagtacta cggcggtccg acttactaca tggagtgcgg acttggcgag gagcgccact
     2701 ggggcaagct gtggctgatc ccggcgctca tcttcggcat cggtattttc tccaccttct
     2761 tcgtaacctc ttccaacctg accgcatctc aggtcgtagc cggtgcgttc ggtattgaga
     2821 atatcaatct tggaagcttc aaagtagaag gcgttatcgt gatgggcgtt ctgctctgca
     2881 ttctgaccta tgtcgttaca agcggcggta cgaagaaaat cgcaagtctg ttcagcaagc
     2941 tcgttccatt tatgagcgtt ctttatatct tgatgggaat tggcatgatc atcatcaaca
     3001 tcaaccgcgt tcctggcgta tttgcagcca tcgtaacaaa cgcatttacc ggaacagcag
     3061 cgatcggcgg ttttgccggc tgcgcagtca gcgagattat ccgtgtgggt atggcgcgtt
     3121 ccgtatactc caatgaggct ggatggggta cttctccaat gatccatgct tccgcaaaaa
     3181 caccgcatcc ggtagagcag ggtctgtggg gatctttcga ggtattcttt gataccatgg
     3241 ttatctgtac gatcaccgca ctttccgtta tcttaagcgg aaactggacc gacggaacga
     3301 acggcggaac acttgcttta agtgcatttg caagcggatt cggcaaattt ggaagcatcc
     3361 ttctggcagt catcatggtc attttcactg taacgacttc cggcggatgg ttcacctact
     3421 atctggcaat tctggagcat ctgtataaaa aagaaggcaa gatgaaagat atcgtcatga
     3481 aaatcttcta cgcagtccgc gctctgccgg gcgtactgtg gacgatctat ctggtaaaga
     3541 ccaacaacca gggctttatc tggactgtcg ttgatatcac atctgcgatc ccgaccttcg
     3601 tcaacgtagc agtgatcctg attctctcga accagtattt caaactgctg aaagactaca
     3661 aagcaagaaa cttcggccag ggcacggtag acaagaacgc ctgcctgttc tacgaagaga
     3721 aaacgaaata aaaaatattc aaaagcgaat ccagacacgc aagagaatag cgtgtctgaa
     3781 tttgtatatt gcgcaaatgg gcagaaaaga atagaatcaa atttagatga cagtaggagg
     3841 ataatgatat gaaaacaatt tacaaaaacg gagctgtcta caccggaaaa ctgccgcttg
     3901 tcgaagcgtt tgcagtagaa gacggcaaat ttgttttcgc cggaagcagc gcagaggcag
     3961 atgcccttgc agcagccggc gacaaagttg tagacctcgg cggaaaattc gtctgcagcg
     4021 gttttaacga cagccatatg caccttttaa attacggaag ctccctgctg aaagcgcagc
     4081 ttgccgcaca taccgaaagc ctggaagata tgctcgcctg cctcgacacc ttccagaaag
     4141 agcatccgcg aaaagacggc gcatggctga tgggccgcgg ctggaatcag gattacttca
     4201 cggacgtgga ccgcatgccg aaccgctacg atcttgaccg cgtatcaact gaagttccga
     4261 tctgcgcgac gagagcgtgc ggccactgcc tcgtagtcaa cagcaaagcg ctggaactcc
     4321 tcggcgtcac cgcagatacc ccgcagccgg agggcggaga gatcggtatg gaaaacggtg
     4381 agccggacgg aagattcttc gacaatgcaa tggaaccggt ttacgacagc attccggtgc
     4441 cgagcaaaga ggaaatcaaa gatatgatcc gcagcgcctg caaagccctg aacagctacg
     4501 gcgtcaccag cagccagagt gatgattact gcgtttaccg cgccgttcca tggcagaccg
     4561 tcaacgaagc atacaaagaa ctcgaggagt ccggcgagct gaccgtccgc gtttacgagc
     4621 agagcaactt taccgatctg gaatccttaa aaggtttcgt tgaggctgga aatgtgaccg
     4681 gcatcggcac agaccgcttt aaaatcggac cgctgaaaat gctcggcgac ggcgcactcg
     4741 gtgcgagaac agcattcctc agtcgcccat acgcagacga cccgtccacc tgcggaatcc
     4801 cggtattcac ccaggaaacc atggatgaga tgatcggcta cgccaatgca cacggcatgc
     4861 aggctgcagt tcacacgatc ggtgatgcgt gcctcgaccg cgtgttaagt gcctatgaaa
     4921 aagcccttgc tgaaaacccg agagaagatc accgccacgg catcgtgcac tgccagatca
     4981 caagagccga tcagcttgaa aaaatcgcga aaatgcatct tcacgtttac gcacagagca
     5041 tcttcctcga ttacgataac catatcgtag aggagcgcgt cggcaaagag cttgcgagca
     5101 caagctacag ctggaagacg ctgatgaata acggcgtctc cgtcagcaac ggaactgact
     5161 gcccggtaga gctgccggat gccctcgcct gcatgcagtg cgctgtcaca agaacaacgc
     5221 tgcgcgacca tgtcggcccg tacctgccgg atcagaaatt caccgtacag gaagcactcg
     5281 acagttacac gatccgcagc gccgaggcaa gtttcgagga gaacgtgaaa ggccagatcg
     5341 cagaaggcat gatggcagac ttcgtcgtac tgggtcagaa tccgtttgag gtggatgaga
     5401 gcacgatcaa agacattccg gtgtgccgga cgtatcttgc cggtgaactg gtatatgaag
     5461 cataagaata taactcaaat gagtaataaa tgtcaaatga aaattcatac aatgaaggga
     5521 aaaacgtgaa ataagataac ctgaaaatag tatggaataa aggcagaaac agaggagaac
     5581 ctcctgcggc gatgaatggt cgcaggaggt tcttttttga tcctgatagg accatacggt
     5641 cgtaaaagaa tcgaacaaat ggttgcataa aagggaggga atgtgctatg ataaaggaaa
     5701 ttttacaaaa ggaaaaaaca gacaaatgct tggaagaaca cattttttta ttggaacagc
     5761 agcggcactt gctgttttac agccgcagac ggttcccgcg cttgtggccg gtgccggggc
     5821 tgccgcgatc ggcggtttga tcagtgacat tgatgtcggc acttcacagg cgcaccgcga
     5881 tgccgataag atcattacgg caacagttgc agttgcggtg ctcacaattc ttgcggagta
     5941 taaactgaat cttggaattt accgccgcct cacaagcgac agcagcgtgc tgcgtctcct
     6001 cgccggaacg gcagcatttt tgctgatctg cgcctatggg aaacagcagc cgcaccgctc
     6061 cttcatgcat tcatttgcgg cgctggcatt gctgacggcg tgcgtggaca tcatctaccc
     6121 ggatgtgtcc gcttattttg ccgtgggctt tctgtcccat ctggtgctcg attttttcaa
     6181 ccggaaaccg gaaaagctgt tctggccctg gaagaaggga ttctgcctcg gactctgctc
     6241 agcacgggga cttgtgaacc gcgcactgct cgggtgcggc atggtatctc ttgctgtaat
     6301 actggtgatc tcggcgcctg cagggcggct gatggctaaa atcatgcggg caatttacgg
     6361 atgagatggg attgacaaaa agataacaag acaaaggaag aaaaagcagg aatttttcgg
     6421 tgttaaagtt aatattttat acattttata gtaggatttt tgattaaaat gatgtaaaat
     6481 agaaaacaga aatccggcgt ccgtaattgt ttttgaaatg aacgaacagg gaaccgccgg
     6541 cccgttaccg cagccgattg cggagcgcag cgcaaccgac aacagagatt agagcacaac
     6601 aggcaataca ggagttagta tgaacgaaga aatgaaaaac caaacagaac agttaccgct
     6661 tttaaaagta cacatgatgg gaagtttttt cctggagtac ggcgaccagc cgatttcctt
     6721 ccggaaaaca atggcaacaa aatcgatgag acttctgcag attttcctct ataacaagga
     6781 caatggaatt tccagaggaa gactgttgga gagcacatac ggaagagaag atgtggcgga
     6841 cgcggcaaac agcctgcgtg tcggcgtaca tcgcctgaaa agaagccttg tcgaggcagg
     6901 acttccgcag tttgagtaca tttatacaca gaaaggcgtt taccactgga ccagcccgat
     6961 gccggtcgag gtagatgcga ttgatatcaa aaataagatc ctggaagcag gaaaagagac
     7021 agacgagaaa gcgcgcatgg atcagatgat cgaagcgctg gagctgtaca ccggagagtt
     7081 ccttgcagat ttcggcgagg aagagtgggt acaggcggag cgcgcgcagc tgaaaaaagt
     7141 atattccgat gcactgaaag aagtaagtga gtatcttctg aaaaacgagg agttcgatga
     7201 gctgcagaaa ctgacttctg ttgcttccga actgtatccg ttcgacgagt ggcaggcagt
     7261 ccagatgcag gcgctgatcg gcctggagag atacaaagag gccatgaaac tgtacgagca
     7321 gacctcgaaa cattactttg aagagcttgg cgttacaccg agcgagaaac tggtggagca
     7381 gtaccgctat ctgggaagcc gcatgggcag cagacaccgc gtgatcgaag aggtacaggc
     7441 agatcttcag gagagcccgg gagaaaaggg cggagcattc ttctgttccc tcgccggatt
     7501 ccgtgactgc taccgacttg tttaccgtat gtccgagctg aacggacaga tgccgtggct
     7561 gatgctgtgc accattacag acggaaaagg ttacccggca aaaggcggtc cgcgccttga
     7621 ccgtatgtcc gaaaagctgc ttgaggtaat gaaacgcagc ctgcgtcatt ccgatttctt
     7681 tgcaaaatac agcccgtccc agtatgtgat tctgctgcag ggcggcagcc agagaggatg
     7741 cgaactcgtc tacaagagaa tcagcgagcg cttctccgag gagaaaaaag gctgggcaaa
     7801 gaatctgaaa tacagtgcac tgccggcaat tcagaccgaa aaagagcttg agttcttaga
     7861 aggagacgat gttttatgat tcgcgtacag ctgcttggaa gattccgcat gtacgatgaa
     7921 acacgctgcc tcaacgagga agcgattcat tccccgatga tcacaaagct gctggtttat
     7981 atgatctgca acaaaaacca catcgtaaca ctggaacagt taaccgaagc actctggacg
     8041 gatgaggaga gtgaaaatcc gtacggagca ctgaaaaata tgatgtaccg tctgcgcgga
     8101 acgctgcgga aggtatggcc ggttgagaga ggatcatgcc tgctatatga aggctgtcat
     8161 ggaactgacg aaaagcctgg agcaccgcag accgcaaagg aaaagtacag gttctcagtg
     8221 atctaaaaga gctttatcag gaaccaagag aattagaaaa aataaattaa tttttaagtt
     8281 gacagcagac taaacaaatg gtaactttaa gctgtgatga aacgagctta aatccagaaa
     8341 aagtctgctg tcttaagtta aataaagtac aacagacagg gaggataaca aacatgagtt
     8401 acagagaagt atatgaaaaa tggtgcaatg atccatattt tgatgaggca tccagagaag
     8461 agctgaaagc actcgaagga aatgaggcag aaatcgaaga ccgtttctac cgtcagcttg
     8521 agttcggtac cggcggactc cgcggtgtca tcggagcggg taccaaccgc atgaacatct
     8581 acaccgtacg ccaggcaacc cagggccttg ccaactacat catttcccag aacggacagg
     8641 aaaaaggcgt agccatcgca cacgattcca gaagaatgtc tccggaattc gcacgcgaag
     8701 cagcactctg cctgaatgca aacggaatca aaacgtatct gttcgagtcc ctgcgtccga
     8761 caccggagct ttcttttgct gtccgtgaat tcggctgcat ttccggtatc gtcattaccg
     8821 caagccacaa cccgagagag tacaacggat acaaagtata ctgggaggac ggcgcacaga
     8881 ttacaccgcc gcacgacaaa aacatcctcg cagaggtagc aaaagtaacc gagttttctg
     8941 ccgttaaaac gatggaagaa gatgatgcaa gagcagaagg cctgttccag gtcatcggaa
     9001 ccgattttga tgaccgttac gtcgcaaaat taaaagagca gagcattcat ccggaaatca
     9061 tcaaagatgc ggcaaaagat atgaagatcg tctacacacc gcttcacgga accggaaatg
     9121 tgccggtacg ccgcgttctg cgcgagcttg gctttaatca ggtttacgtt gtaaaagagc
     9181 agaaagtgcc ggacggaaac ttcccgacgg tggcttaccc gaacccggaa gacgagaaag
     9241 catggacact ggcgctgaaa ctggcaaaag aagtagacgc tgacatcatc cttgcaaccg
     9301 accctgacgc agatcgtctc ggcgtttatg caaaagacag caagaccggc gagtacgtaa
     9361 gcttcaccgg aaacatgtcc ggtatgctga tcgcagagta tattctgcgt gagcgcacca
     9421 agaacggaac catgccggag aacccggcac tcgttgagac gattg
//
LOCUS       DAOMUF010000073         9436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1810_length_9436_cov_4.398572, whole genome shotgun sequence.
ACCESSION   DAOMUF010000073 DAOMUF010000000
VERSION     DAOMUF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1810_length_9436_cov_4.398572"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /gene="metK"
                     /locus_tag="PLN77_05685"
     CDS             complement(<1..1138)
                     /gene="metK"
                     /locus_tag="PLN77_05685"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRM43804.1"
                     /translation="MEKRLFTSESVTEGHPDKICDAISDAILDALLEQDPMSRVACET
                     CTTTGYVMVMGEITSNAHIDFQQVVRDTVREIGYDRAKYGFDADTCAVVTAIDKQSTD
                     IAMGVDKALEAKENNMSDEEIDAIGAGDQGMMFGFATNETEEYMPYPIYMAHKLARQL
                     TKVRKDGTLSYLRPDGKTQVTVEYDENGKPFRLDAVVLSTQHDPEVSQEQIHEDIKKY
                     VFDEIIPADMVDEHTKFFINPTGRFVIGGPHGDSGLTGRKIIVDTYGGYARHGGGAFS
                     GKDCTKVDRSAAYAARYVAKNIVAAGLADKCEVQLSYAIGVAHPTSIMIDSYGTGKLS
                     DNKLTDIVRENFDLRPAGIIKMLDLRRPIYKQTSAYGHFGRNDLD"
     regulatory      complement(1265..1376)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1599..3227)
                     /gene="ptsP"
                     /locus_tag="PLN77_05690"
     CDS             complement(1599..3227)
                     /gene="ptsP"
                     /locus_tag="PLN77_05690"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRM43805.1"
                     /translation="MQYFQGKSVYKGIVMGPVAVLKKNDYQVKRARIEDPEAEVKRVK
                     EAVEVSKKQLGRLYDKAVREVGEASAAIFEVHQMMLEDEDYLESMENMIRIELVNAEY
                     AAAATGDNFAEMFAAMDDEYMKARSADVKDISERLVRNLSGEGDNDLSSMEPSIIVAD
                     DLSPSETVQMDKEKILAFVTVHGSTNSHTAILARMMNIPALIGVPMDLNGLKTGMTAV
                     VDGFSGQVIFEPEEDVQKETEKRMQEEAEKQKLLEELKGKENITPDGRKINIYANIGS
                     VGDLGYVMENDAGGIGLFRSEFLYLGRNDFPTEEEQFQAYKQAVQTMAGKKVIIRTLD
                     IGADKQVEYFNLGKEENPALGYRAIRICLKQPEIFKAQLRALFRAAVYGNLSVMYPMI
                     TSTEEVEKIYAIVAEVEEELKKQEVQYKIPEQGIMIETPAAVMISDRLAEMVDFFSIG
                     TNDLTQYTLAIDRQNEQLDDFYNPHHEAVLRMIRMVVENAHKCGKWAGICGELGADLT
                     LTEQFVRMGVDELSVAPSMILKLRKVVREMKAEE"
     gene            complement(3251..3508)
                     /locus_tag="PLN77_05695"
     CDS             complement(3251..3508)
                     /locus_tag="PLN77_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM43806.1"
                     /translation="MKSFEYTIKDELGIHARPAGMLVKEAKNFASECTITKDGKTKKL
                     TQLMMLMSLGVKQGDTVTVAANGEDEDAAIETLKAFFEANL"
     gene            complement(3513..4349)
                     /locus_tag="PLN77_05700"
     CDS             complement(3513..4349)
                     /locus_tag="PLN77_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM43807.1"
                     /translation="MYRIAKTLNHNSFIGVEEENNREYLVMGKGIAFGKKTGQRVQPG
                     DDARIYSLTEMTERGNARTIVQGVSPLSLELASAVLDEAEKEFGKIDRSIVFPMADHL
                     DFAVRRIQNGEQISNPLTDDIRVMFYKEYKVASCIRELLKERLQIEIDEHEIGYLALH
                     VHSAIVEENVSQAMEVARAVRESISLVEHITGHTIDVMSLSYNRMMNHIRYMVARAVS
                     GEKLKVNMNDYMSVKFPKAFQAAKRICEEMEKRLKLPMEEIEIGYLAMHIERIMDREE
                     GE"
     gene            complement(4377..6554)
                     /locus_tag="PLN77_05705"
     CDS             complement(4377..6554)
                     /locus_tag="PLN77_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM43808.1"
                     /translation="MKDKIFSVLQRVGRSFMLPIAILPVAGLLLGIGSSFTNATTIET
                     YGLTSLLGNGTILHALLVIMNKVGSAVFDNLPLIFAVGVAIGMAKKEKEVSALSAVIA
                     YFVMNTAINAMLTITGQILENGEIAESVLEGTITSVCGIQSLQMGVFGGIIVGLGVAA
                     LHNRFYRIQLPNALSFFGGTRFVPIISTIVYMFVGILMYFVWPVVQNGIYALGGLVTG
                     SGYVGTLIFGLIKRALIPFGLHHVFYMPFWQTAVGGTMEVAGQMVQGGQNIFFAQLAD
                     SANVAHFSADATRYFSGEFIFMIFGLPGAALAMYHCAKPEKKKAAGGLLLSAALACMA
                     TGITEPLEFSFLFVAPALFVVQVILAGTAYMIAHILNIAVGLTFSGGLLDFFLFGILQ
                     GNEKTSWMRVIPVGILYFFLYYFIFKFMIKKFDFKTPGREDDDVETKLYTKADVNARK
                     EAQNDGVASEDAMSEAITKGLGGKKNISDVDCCATRLRCTVHDAARVSDGILKATGAS
                     GVVHKGNGVQVIYGPNVTVIKSNLEDYLEKAPNTYAEAENAEAENAPAQKEAEPAQET
                     KEQKVVDTITVCSPMSGLAADLSTAPDEAFAEKMMGDGAVVTPEDPYVRAPEDGEVAF
                     VFDTKHAIGFVTDSGVSLLIHVGIDTVKLNGEGFEALVESGQTVKKGDPMLKLDLEYL
                     KTHAPSVTSPVLCTELEENQRIRLLTDGPVKAGDPLFAIEILA"
     gene            complement(6846..9038)
                     /locus_tag="PLN77_05710"
     CDS             complement(6846..9038)
                     /locus_tag="PLN77_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRM43809.1"
                     /translation="MSNEAETQYNPQCGVRRTIFSDEYDFLTKIESVCAKEDGIHFLV
                     RTWKDRIATVRITFLTPSVFRFVMVPEMTAKSHRQTVVEDVPESEFETKNAEFTENED
                     LYIYETNQLSLHFSKNYWEMSIYQNGKLLTKEQAFDTNVDNRWKQLPTGFRVDASGKS
                     IASFEQFVLFSDEQFWGFGEKFTHFNKRGQRIECWQKDALSTNTEDSYKSHPYFTSSR
                     GYSVLLNTFTRSSFDMGASSWISYQMGSDDPILDYIFLAEESKDYKKLLQQYLQITGQ
                     IPMIPKWAFGFWMSKCSYMSRKEVEDVVADAEKFGIGIDVIHIDGWQRQDMSGVWEWD
                     TERFPEPEEMIKELNKKNIHLSLWNYPYLQEDSPAFKELAERGFFIKNKEGHPAMFKA
                     TADSEFLCACFDFTNPEFLAWYEERVKKIVRMGVSVIKTDFSEAVPEDVVFYNGMSGR
                     EGHNLLTYLYAKNIYTWMKEICDERGELPMLWGRSGYVGSHTIPAAWAGDSSSDKASH
                     SAILQAGLGMAMSGVSFWGYDLGGFYNTGYIGNEERPNIEDYLSSVQMGLWMPLSRAH
                     GKTPREPWQYGDMALKEVKKWINFRHRLVPYLYHTACQSHQSGIPMIRPLVMEYPKDP
                     IAKTQNLSYMLGDALLISPGFDRDEYELYLPEGRWQDIESKEVYEGMTFVHIENKAFA
                     DGGTSLRVFQKEGTSIPLFAQKEVLHVPAQLWKQEDLEFMTFQKKDVD"
     gene            9295..>9436
                     /locus_tag="PLN77_05715"
     CDS             9295..>9436
                     /locus_tag="PLN77_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM43810.1"
                     /translation="MFTINKNGLSYGRGYVYSLQYHLVWCTKYRKKVLKDGIDVECKE
                     MLE"
BASE COUNT         2407 a         2368 c         1738 g         2923 t
ORIGIN      
        1 gatccagatc gtttcttccg aagtggccgt aagcggaagt ctgtttgtag atcggacggc
       61 gcagatccag cattttgatg attccagccg ggcgaagatc aaagttctcg cgaacgatat
      121 cggtcagttt gttgtcggac agttttcctg tgccgtagct gtcgatcatg atagaggtcg
      181 gatgagcgac accgattgcg taggaaagct gaacctcgca tttatcagca agacctgctg
      241 ctacgatgtt ctttgctacg taacgtgctg cataagctgc agaacggtct acctttgtac
      301 agtcttttcc ggagaaagct ccgccgccgt gacgagcgta tccgccgtag gtgtctacga
      361 tgatcttacg gccggtcaga ccggaatctc cgtgaggtcc gccgattacg aaacggccgg
      421 tcgggttgat gaagaacttg gtgtgctcat ctaccatgtc tgccggaatg atttcgtcga
      481 atacgtattt tttgatatcc tcatgaatct gctcctggga tacttccgga tcatgctgag
      541 tggaaagaac aaccgcatca agacggaacg gtttgccgtt ctcatcgtac tctacggtta
      601 cctgtgtttt tccgtccgga cgcaggtaac ttaaagtacc gtctttgcgg actttggtca
      661 gctggcgagc cagtttgtgt gccatgtaga tcgggtatgg catgtactct tcggtctcgt
      721 tggttgcgaa tccgaacatc attccctgat ctcctgctcc aatggcatcg atctcctcat
      781 cgctcatatt gttctctttt gcttcgagcg ctttgtctac acccatagcg atatcggtgg
      841 actgtttgtc aattgcagtt acaacagcgc aggtatctgc gtcaaatccg tattttgcac
      901 ggtcgtagcc gatttcgcga acggtatcac gtacaacctg ctggaaatca atgtgtgcgt
      961 tggatgtaat ctcacccatt accataacgt atcctgttgt agtacaggtt tcgcaagcta
     1021 cacggctcat cggatcctgc tcaagcagcg catccagaat cgcgtcagaa atggcatcac
     1081 agattttgtc cggatgtcct tcggttacag actcagaagt aaacagtctt ttttccattc
     1141 tttttctcct ttttttcttc tcttctcttt tgcttttcgt tcgtgggctt tggctttccc
     1201 tgaacatttt ttcgtaaaaa aagatccgca atttttagcg gacctgaata gtaatgttca
     1261 aatcccctta ttgttcgaaa atttcgctca ggctggcacc ttcccatgca gaccaatctg
     1321 ccgggggttg ccgtggtttc acagatccta tgtatctcca ccactctgaa taagagaaaa
     1381 gcaactcaat ttttgatttt gttttttgtt actctgatac actatcgcaa aatacgccat
     1441 tcgtcaagag gaaataaaag gatttgagac attttcttac tgttgtccca aacatccttt
     1501 ctttttttct gatttttcgg ctgtatcttc tgttatttgt gaacagataa gaaaactctc
     1561 caaaacagag agttttctta tctgctgtat cgttttttct actcttctgc tttcatctcg
     1621 cggacaacct tccgcagctt cagaatcatg gacggagcaa cagaaagctc atccactccc
     1681 attcgtacaa actgctccgt caatgtcagg tctgcaccga gctccccgca gattcctgcc
     1741 cattttccgc atttgtgtgc attttccacc accatgcgaa tcatccgaag aactgcttca
     1801 tggtgtgggt tgtaaaaatc atccagctgc tcattctgac ggtcaatcgc cagggtatac
     1861 tgcgtcaggt cgttcgttcc gatgctgaag aaatccacca tttccgccag acggtcgctg
     1921 atcatcacgg cagccggtgt ctcgatcata atgccctgct ccggaatttt atactgcact
     1981 tcctgttttt tcagctcttc ctctacttct gcaacaattg cgtagatttt ttctacctct
     2041 tctgttgatg tgatcatcgg atacatgaca gaaaggtttc cgtaaacggc tgcgcggaac
     2101 aatgcgcgaa gctgcgcttt aaaaatttcc ggctgtttca gacaaatccg gattgcacgg
     2161 tacccaagcg ccggattttc ttcttttcca agattaaagt attctacctg cttatctgcc
     2221 ccgatatcca gcgtacggat aatcactttt ttgcctgcca tggtctgcac ggcctgttta
     2281 tatgcctgaa actgttcttc ctccgttggg aaatcatttc ttccaagata gagaaattca
     2341 cttcggaaaa gtccgattcc gccggcatca ttttccatca catagccaag gtcaccaaca
     2401 ctgccgatat ttgcatagat gttgatctta cggccatccg gtgtaatatt ttcttttcct
     2461 ttcaactcct caagaagctt ctgtttttct gcttcttcct gcattctttt ttcggtttcc
     2521 ttctgcacat cctcctccgg ctcaaagata acctgacctg aaaaaccgtc tacaacggcc
     2581 gtcatgcctg ttttcagacc gtttaaatcc atcgggacgc cgattaatgc cggaatattc
     2641 atcattcgcg caagaatcgc agtatgagaa ttggtagatc catgcacagt cacaaacgca
     2701 agtatttttt ctttgtccat ctgtacagtt tcactcggac ttaaatcgtc tgccactata
     2761 atagatggtt ccatagagct caggtcgtta tctccctctc cgcttaaatt gcggaccaga
     2821 cgttcggaaa tatcttttac atctgcggaa cgtgccttca tatattcatc atccatagct
     2881 gcaaacatct ctgcaaaatt atcgccggtt gctgccgctg catattctgc atttaccagc
     2941 tctatccgga tcatgttctc cattgattcc agatagtcct catcctccag catcatctga
     3001 tggacttcaa agatcgccgc acttgcctct ccaacttccc ggactgcctt gtcatacagt
     3061 ctgccaagct gttttttcga aacctccacg gcttctttca cacgttttac ttctgcttcc
     3121 ggatcttcaa tccgggctct tttaacctgg taatcatttt tcttcagaac cgcaaccggt
     3181 cccataacaa ttcctttata tacggatttc ccctgaaaat actgcatttt ttatcctccg
     3241 agcgtacttt ttacaggttt gcttcgaaaa acgctttcag cgtctcaatc gctgcatctt
     3301 cgtcctctcc gtttgctgca acggttacgg tatctccctg ctttacacca agtgacatca
     3361 gcatcatcag ctgagtcagt tttttcgttt ttccatcttt tgtgatggtg cattccgatg
     3421 caaaattttt tgcctccttt accagcattc ctgccggtct tgcgtggatt cccagttcat
     3481 ctttaatggt atattcaaat gatttcatgc ttttattctc cttcttctct gtccataatc
     3541 cgttctatgt gcatagcgag atatccaatt tcgatctcct ccatcggaag tttcagcctc
     3601 ttttccatct cctcacagat tcgtttcgcc gcctgaaagg ctttcggaaa ctttacggac
     3661 atgtagtcat tcatatttac tttcagtttt tccccactga ccgctcttgc aaccatatag
     3721 cggatatggt tcatcatacg gttatacgac aacgacatca catcgatggt atgcccggtg
     3781 atatgctcca ccaaagaaat gctctcccgg actgctcttg ccacttccat ggcctgtgaa
     3841 acattttcct ccacaattgc cgaatgaaca tgaagtgcaa gatagccgat ttcatgttca
     3901 tcaatttcga tctgaagccg ttcttttaaa agctccctga tacaggaagc aactttatac
     3961 tctttgtaaa acatcacacg gatatcgtcg gtgagcggat tgctgatctg ctcaccgttc
     4021 tgaatccgcc ggacagcaaa atccagatga tctgccattg gaaagacgat ggaacggtca
     4081 atttttccaa attccttttc tgcctcgtca agcacggcac tcgccagctc caggctgagc
     4141 ggagaaacgc cctgtacaat ggttctggca tttcctcgct ccgtcatctc tgtcagggaa
     4201 taaatgcggg catcgtctcc cggctgtaca cgctgtcccg tttttttccc aaatgcaatt
     4261 cctttcccca tgaccagata ttcccggtta ttttcttctt ccactccaat aaatgaattg
     4321 tggttcagtg tttttgctat cctgtacatt cttctcaccc gtttttaagg cacagcttac
     4381 gcgagaatct caatcgcaaa taaaggatct cctgctttta ccggtccgtc tgtcaaaaga
     4441 cggattctct ggttctcctc cagctctgtg caaagaaccg gtgacgtaac cgacggcgca
     4501 tgtgttttca gatattccag atccagcttc agcatcggat cacctttttt cacggtctga
     4561 ccgctttcta ccagtgcctc aaatccctct ccgttcagtt ttactgtatc aattccgaca
     4621 tgaatcagaa ggctgacccc ggaatcggtt acaaatccaa ttgcatgttt tgtgtcaaaa
     4681 acaaacgcaa cttctccgtc ctccggagca cgcacataag gatcttccgg tgttacaaca
     4741 gcgccgtctc ccatcatctt ttccgcaaat gcttcgtccg gcgcagtaga aagatcggca
     4801 gccagtccgc tcatcggact gcagacagta atcgtatcca caaccttctg ttcttttgtt
     4861 tcctgcgctg gctccgcctc tttttgggct ggtgcatttt ctgcttctgc attttctgct
     4921 tccgcatacg tattcggagc tttttccaga taatcctcta aatttgattt tatcacagtt
     4981 acatttggtc cgtaaataac ctgtacacca tttcctttat gcacaacgcc agatgcccct
     5041 gtcgccttta aaataccatc gctgactctc gctgcatcat gaacagtaca gcgcagtctt
     5101 gttgcacagc agtccacatc cgaaatattt ttctttccgc caagaccttt tgtgatcgct
     5161 tcactcatgg catcttccga tgccacccca tcgttttgcg cctcttttct tgcatttaca
     5221 tctgctttcg tataaagctt ggtttctaca tcatcatcct cacgacccgg tgtcttaaaa
     5281 tcaaactttt taatcatgaa tttgaagata aaataataca ggaagaaata aagaatgccg
     5341 accggaatca ccctcatcca gcttgtcttt tcatttccct gcagaatacc gaacaggaag
     5401 aagtccagaa gtccgccgga aaaggtaaga cctactgcga tattcaggat gtgtgcgatc
     5461 atataagctg ttcccgcaag aataacctgc acgacaaaca acgccggtgc aacaaacagg
     5521 aaagagaatt ccaacggttc tgtgataccg gttgccatac acgcaagtgc tgcggaaaga
     5581 agcaaaccgc ctgctgcttt tttcttctcc ggtttcgcac aatggtacat ggcaagtgcc
     5641 gctcccggaa gtccgaaaat cataaaaata aattctccgg agaaatatct cgttgcatct
     5701 gcactgaaat gtgcgacatt cgcagaatcc gcaagctgtg cgaagaaaat attctgtccg
     5761 ccctgcacca tctgtcctgc cacttccatg gtacctccga ctgctgtctg ccagaacggc
     5821 atatagaata cgtgatgcag accaaatgga atcagtgctc gcttaatcag accgaaaatc
     5881 agcgttccga cataaccgga gccggttacc aggccaccca gcgcataaat gccgttctgt
     5941 acaaccggcc atacaaaata cattaaaatt cccacaaaca tatagacgat cgtagaaata
     6001 atcggtacaa aacgagtacc tccaaagaaa gaaagtgcat tcggaagctg gatccggtaa
     6061 aaacgattat gaagtgcagc aactccaaga ccgacaataa taccgccaaa cacacccatc
     6121 tgcagtgact gaataccgca gacagaagtg atggttcctt ccagaacgga ttctgcaatt
     6181 tctccgtttt ccagaatctg acccgtaatg gtcagcattg cgttaatcgc tgtattcata
     6241 acaaaatacg caataacggc tgacagggca gaaacctctt tttccttctt tgccatacca
     6301 attgcaacac ctaccgcaaa aatcagcggc aggttatcaa acactgcact tcctactttg
     6361 ttcatgatca ccagcagtgc atgaagaatt gtgccgttgc cgagcaagct tgtcagcccg
     6421 tacgtttcaa tcgtggttgc attggtaaat gagcttccga ttcccagcaa aagtccggct
     6481 accggaagga ttgcgatcgg gagcataaag cttcttccca ctcgctgcag aacactaaaa
     6541 attttgtcct tcattttttt actccttgtg tagtttatac tttttacaag aagagtttct
     6601 gcggttttgc atctttttag ccggtctgta ctctttttct gaaacagagt acaaaaaaga
     6661 gacactaacc cacataaaca attcctgtta tgtatagtta atgcctccaa aaatcgagtt
     6721 acacactaca actgtagttg aatatagcac agataaattt cttttgtcaa gtcagttctt
     6781 attatttcag ctttttattt ttacccattt ttcatatttt tatgcatcta ttttgtgcac
     6841 aatagctaat caacatcttt tttctgaaat gtcataaatt caagatcttc ctgtttccat
     6901 agttgtgcag gcacatgcaa tacttctttt tgagcaaaca atggtatact tgttccttct
     6961 ttctggaaaa cccgaagaga tgtaccacca tcagcaaaag ctttgttttc tatatgaaca
     7021 aatgtcattc cctcatatac ttctttactt tcaatatcct gccaccggcc ttccggaaga
     7081 tacagttcat attcatctct gtcaaatccg ggtgaaataa gcaatgcatc accaagcata
     7141 taagacagat tctgtgtctt tgcgattgga tctttcggat attccatcac aagcggacga
     7201 atcattggaa taccagactg atgactctgg caggcggtgt gataaagata tggcacaagt
     7261 cgatgtctga agttaatcca tttttttact tcttttaatg ccatatctcc atactgccat
     7321 ggttcccgcg gtgttttacc gtgcgctctg gataatggca tccacaatcc catctgcaca
     7381 gaggaaaggt aatcttctat attcggacgt tcctcatttc cgatataacc tgtgttataa
     7441 aatccgccaa gatcataacc ccagaaagat acgccagaca tagccatccc aagtccggcc
     7501 tgaagaattg cactgtgact tgctttatca ctggaagaat ctcctgccca tgctgccgga
     7561 atcgtatgag aacccacata cccactgcgt ccccaaagca ttggcaattc cccacgttcg
     7621 tcgcaaattt ctttcatcca tgtgtaaata ttttttgcat acagatacgt cagaagatta
     7681 tgtccttctc gcccgctcat gccattgtaa aatacaacat cttcaggtac tgcttcagag
     7741 aaatctgttt ttatcactga tacacccatt ctgacgattt ttttcacacg ttcctcatac
     7801 catgccagaa attccggatt tgtgaaatca aagcaggcac acaaaaactc tgaatctgca
     7861 gtcgctttaa acattgcagg atgcccttct ttatttttta taaaaaatcc acgttctgcc
     7921 agttccttaa atgccggcga atcttcctgc aagtacggat aattccaaag acttaaatgt
     7981 atgttctttt tatttaattc tttaatcatt tcttccggtt cagggaaacg ttctgtatcc
     8041 cattcccata caccagacat atcctgcctc tgccagccat cgatatgaat tacatcgata
     8101 cctattccaa atttttcagc atcagcaaca acatcctcga cttctttacg gctcatataa
     8161 gagcattttg acatccagaa accaaaagcc cactttggaa tcataggtat ctgccctgta
     8221 atctgcaaat actgctgcaa tagcttctta tagtctttgc tttcttctgc caggaaaata
     8281 taatccagaa taggatcgtc actgcccatc tgataactga tccatgatga agcacccata
     8341 tcaaatgatg aacgcgtaaa tgtatttaac aatactgaat atccccggct gcttgtaaaa
     8401 tacggatgac ttttatatga atcctccgta ttagtagaca atgcatcttt ttgccaacac
     8461 tctattcttt gtccacgttt attaaaatgt gtaaacttct ctccaaatcc ccaaaattgc
     8521 tcatcggaga ataatacaaa ctgctcaaat gaggctatac tttttccaga tgcatctact
     8581 ctaaatcctg ttggaagctg tttccaacgg ttatcgacat tagtatcaaa agcctgttct
     8641 ttcgtcaata attttccatt ctgatatatg gacatttccc agtagttttt tgaaaaatga
     8701 agacttaact gatttgtttc atatatatac agatcttcat tttctgtaaa ttctgcattt
     8761 tttgtttcaa actccgattc aggtacatct tcgactaccg tctgtctgtg gctttttgca
     8821 gtcatctccg gcaccatcac aaatctgaat accgatggtg tcaaaaatgt aatacgaact
     8881 gtggctatac ggtctttcca tgtacgtacc aaaaaatgta ttccatcttc tttggcacac
     8941 actgattcaa tttttgtgag aaaatcatac tcatctgaaa aaattgttct tcgcacccca
     9001 cattgcggat tatattgtgt ttctgcttca ttagacatat attgtccccc tctggttttt
     9061 attattttta gtcaaaatgt ataatcggta acgtttttgt attcaatata tcataaattt
     9121 gtatattttt aaatatcttt ttttaccaat actgaccgcc tcagctttga aaacggcgcg
     9181 tcgcattctc gttactttag tgatgagtta gacgcgcaag atttttgata aaaatgagaa
     9241 caaatgtttg atatattcct atgtatatgg tataatgttt ttatgggaaa agatatgttt
     9301 acaattaata aaaatggtct ttcatatggt agaggatatg tatactctct gcagtatcat
     9361 ctggtctggt gtaccaagta tagaaaaaag gttttaaaag acggaattga tgtggaatgc
     9421 aaagaaatgc tggaaa
//
LOCUS       DAOMUF010000074         9222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1887_length_9222_cov_3.597033, whole genome shotgun sequence.
ACCESSION   DAOMUF010000074 DAOMUF010000000
VERSION     DAOMUF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9222
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1887_length_9222_cov_3.597033"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLN77_05720"
     CDS             <1..349
                     /locus_tag="PLN77_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HRM43811.1"
                     /translation="VDVTDRGTRIRLEVIASDDESAPEEERFKTIIPFQIAYAVSIHK
                     AQGLEYNSVKIIIPPYNAEKISHSIFYTAITRAKEKLKIFWSAETMKAIVESFDKEKI
                     GQRTLEFIEKKLE"
     gene            754..1689
                     /locus_tag="PLN77_05725"
     CDS             754..1689
                     /locus_tag="PLN77_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRM43812.1"
                     /translation="MAVNKNGIVVLGAVFVDIKGFPEDIYVPDGRNAGHVEYIHGGVS
                     RNVVEDIANLELRPTFLGIADNTALGEDVVRKLQRHKVNTEYMKEIPNGMGTWLAVFD
                     HNGDLAGSISQRPNLMPILDILEENGDEIIENCDSIVAEIDMDKEIIKKLVQLCEKYD
                     KKLYGVVSNMNIAVSRRDLLQKFDCLICNQIEAGVFFSDDYSEKTPAEMEKILAERLK
                     AANVPAMVVTMGGDGAVYAEADGESGFCPAQKVQVKDTTGAGDAFCAGVSAGLTYGKT
                     LGQAVEIGTRLAASVITSSENVCPRFRPEEFGIEA"
     gene            1702..2010
                     /locus_tag="PLN77_05730"
                     /pseudo
     CDS             1702..2010
                     /locus_tag="PLN77_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
     gene            2305..2826
                     /locus_tag="PLN77_05735"
     CDS             2305..2826
                     /locus_tag="PLN77_05735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM43813.1"
                     /translation="MEEYHKMERYELRTIRQDEMEQGVAIELACFPPNEACSEKSMRE
                     RVQYAPELFLAAVDKETGKIAGTLNGLATNETKFRDAFFDEISLYDPTGENVMLLGLS
                     VLPEYRGQGIARALMEEYSRREQKNGRKQLILTCLQDKVEMYKKMNFRDEGISASTWG
                     GEEWHDMTRKLND"
     gene            2912..3463
                     /locus_tag="PLN77_05740"
     CDS             2912..3463
                     /locus_tag="PLN77_05740"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRM43814.1"
                     /translation="MSRVNFGAKPNMYPLPVLIIATYDKDGNPDAMNAAWGVVTDFNE
                     ISISMGDHKTTDNLAATGAFTVSMATEDTVVGCDYVGIESGRKVPDKFARAGFHATKS
                     EFVNAPLIDELPMALECKVKSFDNGILVGEIVNVCADESVLTDGKIDPKKLKPIAFDP
                     VNNTYLAMGDKVGDAFGSGKALK"
     gene            3604..3921
                     /locus_tag="PLN77_05745"
     CDS             3604..3921
                     /locus_tag="PLN77_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRM43815.1"
                     /translation="MYFPVSALLIEFMILSIVNREDSYGYEISQTIKLVADIKESTLY
                     PILKKLEKAGYVTTYSAEYQGRKRKYYSITEAGKTQMAYLQGEWKSYRDTIDNIIEWR
                     KKE"
     gene            3918..4535
                     /locus_tag="PLN77_05750"
     CDS             3918..4535
                     /locus_tag="PLN77_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HRM43816.1"
                     /translation="MTSGEYLKQLEKYLRKLPQSDYEDAMEYFTEYFADAGPENEQAV
                     IKELGTPKQAAAELMRNLLDKKVDEREAMEKEEKQKEKKKTTGANVVWIAILALFAAP
                     IGAPILISILIVLLCAALCAALLDMSVFLIAVVGVAAGIKMLLRGILAVSVSFGGFCF
                     ITGMGVFMLGISILCAVFGVYFAKWMGRLFVWCTRKVTKKGGSRS"
     gene            4532..5461
                     /locus_tag="PLN77_05755"
     CDS             4532..5461
                     /locus_tag="PLN77_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HRM43817.1"
                     /translation="MKKWTKTALLTGCACCIAGAVLMFSGWASGGKAYAETYDLNAMS
                     GSAKKEDGISTQEKIKLDDFDELQADLTIGDLNILPSGDDSCYLAWRIPSKKGETAVE
                     YRVRDGVLSIEETSAVSDTIYINIDFTEEILSGGKQSETGVILYVPEKKVLKKIEVTM
                     GFGDVQMNGIQAESGRLQNADGDITLFGCDMQNIKCKADYGDVELKSGTWENGSITLE
                     DGDAKIQNTKLSGDVSVENSYGDIELELGKKDLERLEITAKTDFGEIDVPDTMENLMQ
                     KEEDEQSFSYVPDQAAGRITLMTKDGDISLEND"
     gene            5790..6767
                     /locus_tag="PLN77_05760"
     CDS             5790..6767
                     /locus_tag="PLN77_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM43818.1"
                     /translation="MASIRDVAIKAGVGIGTVSRALNGSGYISDETKQKIMQAVEELN
                     YKPNELAQNLFRNKSGFIGIVVKNLSHPFMASLVKHVENNLYTNGYKCMLCEVGDNPN
                     RIEEFVEMLQRNVMDGIICCADIPSSVSLAEIHRPIVMIDRYATEGIPCIHSDHKRGG
                     ELAAQALLSAGCKNVLYFASSATECGCSMDRYRRFAEVCKQHKCKITAIDAGSKIPNF
                     QSGPTIWKKYISHFDGIDGLFTDDVTAAACMKMLQKKGIKIPKKLKVVGYDGLDFTRF
                     LTPELTTVRQNVPEIAKRSVDTIIRMIDGKKIEEMEQIVDVTFRKGEST"
     gene            7019..7174
                     /locus_tag="PLN77_05765"
     CDS             7019..7174
                     /locus_tag="PLN77_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43819.1"
                     /translation="MEQEKHGTAEHVEQENGRRKKISVNFMKNRMHIAQKHKIKIVKI
                     LKMTKKY"
     gene            7316..8257
                     /locus_tag="PLN77_05770"
     CDS             7316..8257
                     /locus_tag="PLN77_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM43820.1"
                     /translation="MKKKTSSPKTAESGKLKKALSCWQLYILLIPALIWLIVFAYYPM
                     YGLIIAFKDFKGRIGIMGSPWADPIFKYFKMFFSTSIAMTAIKNTIIISLESLIIGFP
                     IPVIFALLLNQVQRSGPRRIIQTISYAPYFMSNVVVVSLISVLFSANGLVNHAVMGAG
                     GKAIMFTSLPEYFRKLFIGSNIWQTMGFNAIIFIAALTAISPEYYEAATMDGASRFQK
                     ILYIDLPLIMPTIILMLILQVGNIMNVGYEKAYLMQNGTNTTVSEIISTYVYKVGLQT
                     AQYSFATAVGLFNSVVNFIILVITNAVAKKTSDISIF"
     gene            8277..9188
                     /locus_tag="PLN77_05775"
     CDS             8277..9188
                     /locus_tag="PLN77_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM43821.1"
                     /translation="MANNNTVKKKKVKMSTGDKVFTVINTVIMILVCIAIVYPLYYVL
                     LASVTDPVVVSSGKILLYPEAWYTNGYETTLKYQPLWTGYANTIKYTVLGTLISLVCT
                     IPAGYALSRYDMVGRKPIMFLFTFTMFFSGGMIPMYLTVQQLHIYNTTWAMVLPVAVS
                     AYNLIVCRSFFESGIPVELLEAAKIDGCSDFGFFFKIAIPLSKTIIAVMCLFYATAMW
                     NQFFNALMFLQDDSKMPLQVVLRNLVLMNQSNQMGSSASEMVTKQKLAEQLKYCVVVV
                     SAFPLLCVYPFLQKYFAKGVTIGAVKG"
BASE COUNT         2809 a         1931 c         2383 g         2099 t
ORIGIN      
        1 tgttgatgtg acagatcgtg gaacaagaat acgcttagaa gtaatcgcaa gcgatgatga
       61 atcggcacca gaggaagaac gattcaagac aattattcct tttcaaatcg catatgctgt
      121 ttctattcat aaggcacagg gtttagaata taactctgta aagataatta ttccccctta
      181 taacgcagaa aaaatatcgc attctatttt ttacactgct ataacccgtg caaaagaaaa
      241 actcaagata ttttggagtg cggaaacaat gaaagccatt gtagaaagtt ttgataaaga
      301 aaaaatcgga cagcgtacgc ttgagtttat agaaaaaaag ctagaataat ataatagcct
      361 aatcaaacat acaatgttga gaagataaga tggctgcaaa aggcttgaat tttagcaaat
      421 gtgggtttgt ttggttatag cctaactatt acatgtggag acggtagtct tgatgtcaac
      481 acaaagaaat aaggaccagt ttccaggtcg agacggtgaa atccggcaac tcaactccct
      541 gcgagtgtat ggccaatcag aggtcagagc ggatggattg tgtaggagtt gagcgttctt
      601 cactgtctca actgtcgaga cagtagcaga gagcagcaat gtaaaagtgc cttgcattat
      661 ttggatgctg ataagacaga aatttcgtga aacagacgaa gaggcttgtc tgaaaaaaga
      721 aggaatgaaa aaacaggaga ggaaaaaaga aacatggcag taaataaaaa tggaatcgta
      781 gtattaggag cagtttttgt ggatatcaaa ggttttccag aggacatcta cgtcccggac
      841 ggcagaaacg ccggccatgt cgaatacatc cacggcggtg tgagccgaaa cgtggtggag
      901 gatatcgcaa acctggaact gcgcccgacc ttccttggaa tcgcggataa taccgcactc
      961 ggcgaggatg ttgtgcgcaa actgcagcgc cacaaagtca ataccgaata catgaaagaa
     1021 atcccgaacg gtatgggaac atggcttgct gtcttcgatc acaacggtga cctggcgggc
     1081 tccatttccc agcgcccgaa cctgatgccg attctcgata ttctggaaga aaacggagat
     1141 gaaattatcg aaaactgcga ttccattgtg gcagagatcg atatggacaa agagatcatc
     1201 aaaaagctgg tacagctctg tgaaaaatac gataaaaaat tgtacggcgt tgtcagcaac
     1261 atgaacatcg cagtcagcag aagagatctt ctgcagaaat ttgactgcct gatctgcaac
     1321 cagatcgagg caggcgtttt cttctccgat gattacagcg aaaaaacacc ggccgagatg
     1381 gaaaagattc tcgccgagcg tctgaaagcg gcaaatgttc cggccatggt cgtaacaatg
     1441 ggcggcgacg gagcggttta tgcggaagca gacggagaga gcggcttctg cccggcgcag
     1501 aaagtacagg taaaagatac aacaggagcc ggtgatgcgt tctgtgcagg tgtttccgcc
     1561 ggtctgacct atggaaaaac gcttggccag gcagttgaaa tcggtacgcg tcttgctgca
     1621 tccgtcatca catcgtcgga aaatgtctgc ccgcgcttcc gcccggaaga gttcggcatc
     1681 gaggcataga agggggcaga aatgaagtta ctgtacggaa ccggcaaccc ggcaaaactc
     1741 gacgcgatgc gtcaccgcct tgccggtctt gggattgagc tgatcgggct gaaagatctt
     1801 ggcggtgtta agcagccaga gattatcgag gatggaaaaa cgccgctgga aaatgccagg
     1861 aaaaaggtgg aggcgtattt caacgcgctt catatgccgg tgttctcctg tgattccggt
     1921 ctgtattttg acaacgtggc agaggacgac cagcccggcg tccatgtgcg gaccgtgaac
     1981 ggaaagtatc tgtccgatga agaaatgacc gcgcattacg ccgcccttgc agaaaagtac
     2041 ggcggcctga cgggacgcta caaaaacgca gtttcgctga ttcttgatgc ggaccaccgc
     2101 tatgacgcga tggacccgtc aatggagagc gctcctttcc gcatggtcag cacaccccat
     2161 ccgatgagca aaaaaggctt tccgctcgac cgtctgtcga tcgatctgcg gaccggaaaa
     2221 tattattacg atctgaatga gcaagaagcc gcgctcgatc agcttgccgt ggaagacggt
     2281 tttctgcagt tttttgagcg ggcaatggag gaataccata aaatggaacg atatgaactt
     2341 cgaaccatcc gccaggatga aatggaacag ggcgttgcca ttgagctggc gtgctttccg
     2401 ccgaatgagg cgtgctccga aaagagcatg cgcgagcggg tgcagtacgc gccggagctg
     2461 tttctggcgg cagtagacaa agaaaccgga aaaatcgccg gaacgctgaa cggacttgca
     2521 acaaacgaga caaaattccg tgatgccttt ttcgatgaaa tcagtctgta cgacccgacg
     2581 ggagaaaacg tcatgctgtt gggactcagt gtcctgccgg aataccgcgg ccagggaatc
     2641 gcccgcgcgc tgatggaaga gtacagccgc cgggaacaga aaaacggaag aaaacagctc
     2701 attctgacct gcctgcagga taaggtggag atgtacaaaa agatgaattt ccgcgacgag
     2761 ggaatctccg catccacctg gggcggcgag gaatggcatg atatgacgag aaaattaaac
     2821 gattaagaaa atttgcagag aaaatctctt gtgttaaagt ttgctgtaag cgttataata
     2881 aagaaaatca agcaaaacag gaggaacaac catgagcaga gtaaattttg gagcaaaacc
     2941 aaacatgtat ccacttccgg ttctgattat tgcaacatac gacaaagatg gcaacccgga
     3001 tgccatgaat gctgcatggg gtgtagtcac agatttcaat gagatctcca tcagcatggg
     3061 cgaccacaag acaacagaca accttgccgc aaccggtgcc ttcaccgtca gcatggcaac
     3121 cgaggatact gttgttggct gcgattacgt cggcatcgag tccggcagaa aagttccgga
     3181 taaatttgca cgcgccggtt tccatgcaac gaagagcgaa ttcgtcaacg cgccgctgat
     3241 cgacgagctt ccgatggcac tggagtgcaa agtgaaaagc tttgacaacg gcattctggt
     3301 cggcgagatc gtgaacgtct gcgcagacga gagcgttctg accgacggaa agatcgaccc
     3361 gaaaaaactg aaaccaatcg ccttcgaccc ggtcaacaac acctatctcg ccatgggcga
     3421 caaagtcggc gacgcattcg gttccggaaa agccctgaaa taaacacaga tgatgacatg
     3481 acagcggata tgtaaaaatt ttatatattc cccttgacaa accgcctcca tagtcttatg
     3541 atatgtataa attatatata tataaatatt ttacatatca gaaactaacg gaggtggttt
     3601 tttatgtatt ttccggtatc agcactgctg atcgaattta tgatcctgtc gattgtgaat
     3661 cgggaagatt cttatggata tgaaatcagc cagacgatca aactggttgc agacatcaaa
     3721 gagtcgacac tgtacccgat cctgaaaaaa ctggaaaagg cgggctacgt gacgacgtat
     3781 tccgcagagt accagggcag aaaacggaag tattattcga tcacagaagc aggaaaaaca
     3841 cagatggcgt acctgcaggg cgaatggaag tcctatcgtg acaccattga caacatcatc
     3901 gagtggagga aaaaagaatg acgagtgggg aatatctgaa acaattggaa aagtatctga
     3961 ggaagcttcc gcagagcgac tatgaagatg cgatggaata cttcaccgag tattttgcag
     4021 atgccggtcc ggaaaatgag caggccgtga tcaaagaact cggaacaccg aaacaggcag
     4081 ccgccgagct gatgcggaat cttctggaca aaaaagtaga tgaacgcgag gcaatggaaa
     4141 aggaagaaaa acagaaagag aagaaaaaaa cgacaggcgc caacgtcgtc tggatcgcga
     4201 ttctcgccct gtttgcagcc ccgatcggag ccccgattct gatcagcatt ctcatcgttc
     4261 tgctctgcgc ggccctctgt gcggcactgc ttgatatgag cgtctttctg attgcagttg
     4321 tcggcgtggc cgccggaatt aagatgctgc tgcgcggcat tctggcagtc tcggtttcct
     4381 tcggcggatt ctgctttatt accggaatgg gcgttttcat gctgggaatc agtattctgt
     4441 gcgcggtgtt cggcgtatac tttgcaaaat ggatgggacg gctgttcgtg tggtgtacac
     4501 gtaaggtaac gaaaaaagga ggaagcagat catgaaaaaa tggacaaaaa cagcattact
     4561 gacaggatgt gcctgctgta ttgccggtgc agtgctgatg ttcagcggat gggcaagcgg
     4621 cggaaaagca tacgcggaaa cgtatgatct gaatgcgatg agcggttctg cgaagaagga
     4681 agacggcatt tccacacagg aaaagataaa acttgatgat tttgatgaac tgcaggcaga
     4741 ccttacaatc ggcgacctga acatccttcc gtccggtgac gattcctgtt atctcgcctg
     4801 gcggattccg tccaaaaaag gagagactgc agtggaatat cgcgtgcggg acggtgttct
     4861 gagcatcgaa gagacaagcg cagtgtccga tacgatttac attaatattg actttacgga
     4921 agaaatctta agtggcggta aacagtcgga aacaggagtg attctgtatg taccggaaaa
     4981 gaaggtactg aaaaaaatag aggtaacgat gggattcggt gatgtgcaga tgaatggcat
     5041 ccaggcggaa agcggaagac tgcaaaatgc ggatggagat atcacacttt tcggatgtga
     5101 catgcagaat atcaaatgca aagcagatta cggtgatgtg gaactgaaga gcggaacgtg
     5161 ggaaaatggg tcgatcacgc tggaggatgg cgatgcgaaa atccaaaata cgaagctttc
     5221 cggagacgtg tcggtggaaa actcctacgg cgatattgaa cttgaactgg gtaaaaaaga
     5281 tctggaacgg ctggaaatta cggcaaagac agattttggg gagatcgatg taccggatac
     5341 catggagaat ctgatgcaga aagaggagga cgagcagagc ttttcttatg taccggatca
     5401 ggctgcgggc agaatcacac tgatgacaaa ggatggtgat atctcgttgg aaaacgatta
     5461 agcgtttctg aatgttcagg gacatatgaa attccagaaa aatcgcaaag catgcccgca
     5521 tgaatgagcg tttttggaaa ataagacaaa agtgacaaaa acacaggagg ctgtttggat
     5581 ggtttcacca gaaaaaggga aatgatccaa acagtcgctt gtttttttgt acttaaattt
     5641 tcagctttta acttgatttg tagattgcgg tgctttaaaa taaaagtaat caagcattgt
     5701 ggaacgggct ccacagactg gcagcggggg aaaagccgga acgagtcaga gaaaacagcc
     5761 cagtgtcaga ataggagtta ggagaaacta tggcaagtat tagagatgtc gcgatcaaag
     5821 cgggagtagg aatcggaacg gtatcccgtg cactcaatgg gagcgggtat atttccgatg
     5881 aaacaaaaca gaagatcatg caggcagtgg aggaactgaa ctataaacca aatgagcttg
     5941 cccagaacct gttccgcaac aagtccggtt ttatcggaat tgttgttaag aatctttcac
     6001 atccgtttat ggcatctctg gtgaaacatg tcgagaataa tctgtacacc aatggctaca
     6061 agtgcatgct ctgcgaggtt ggggacaatc cgaaccgcat cgaggaattt gtggaaatgc
     6121 tgcagcgaaa tgtcatggac ggcattatct gctgtgcgga tatcccgtct tctgtcagcc
     6181 tggcggaaat tcaccgtccg attgtcatga ttgaccgcta cgccaccgaa gggattccgt
     6241 gtatacattc cgaccataag cgcggcggtg agcttgcggc gcaggcactt ttgagcgcag
     6301 gatgtaagaa cgtgctctat tttgcatcga gtgcaacaga gtgtggttgc tccatggatc
     6361 gttaccgacg gtttgcggaa gtctgcaaac agcataaatg taaaattaca gcgattgatg
     6421 ccggaagcaa gattccgaat tttcagagcg gcccgacgat ctggaaaaaa tacatcagcc
     6481 attttgatgg aattgatgga ctctttacag acgatgtcac agcggcagcg tgcatgaaga
     6541 tgctgcagaa aaaagggatt aagattccga aaaaactgaa ggtggtcggc tacgacggcc
     6601 tggattttac ccgctttttg acaccggagc tgacgacggt ccgtcagaat gtgccggaaa
     6661 tcgcaaagcg gagcgtggac acaatcatcc gcatgattga cggaaagaag attgaggaga
     6721 tggagcagat tgtggatgtg accttccgaa aaggagaaag tacctgaaga taacaaaaca
     6781 gggaattacc gaattttgca tcattttccg ggattgcgat agaacagaag aagaacagat
     6841 atcagagaca ctcgatctga aaagaaaggg tgtctctttt tcatgtataa aataaaaatg
     6901 aaataaaacc aacttatata cggcaaaagg gcagaaagac tgagcaaaag gatatgaaat
     6961 cgctgctgaa acagttgcaa ttgtcgaaaa aatagtgaaa aacggaaaag tggaacatat
     7021 ggaacaggaa aagcatggaa cagcggaaca tgtggaacag gagaatggga gaagaaagaa
     7081 gataagtgtg aattttatga aaaacaggat gcatattgca caaaaacaca aaataaaaat
     7141 tgtgaaaata ttgaaaatga cgaaaaaata ttgatgattt gttgacattg ggcgaactgc
     7201 gtactatgat acaggtatgg aacaggttcc acaaggcaaa gtgccaaaac ggaaacgatg
     7261 caaatgtgga acgaaaagcg gttggtaaag cttttagaga aataaggagg gaactatgaa
     7321 gaaaaaaacg tcatcgccga aaacggcaga aagtggaaaa ttgaaaaaag cgctctcgtg
     7381 ctggcagctg tacatcctgc tgattcctgc actgatctgg ctgatcgttt ttgcttacta
     7441 tccaatgtac ggcctgatca tcgcctttaa ggattttaaa ggccgcatag gaatcatggg
     7501 aagcccgtgg gcagatccga ttttcaagta tttcaagatg ttcttcagta ccagtattgc
     7561 catgacggca atcaagaaca caatcatcat cagtctggag agcctgatca tcggattccc
     7621 gattccggtt atcttcgcac tcttactgaa ccaggtacag agaagcggac cgagacgaat
     7681 catccagacc atctcctacg caccgtactt tatgtcaaac gtagttgtag tcagcctgat
     7741 ttccgtactg ttttccgcaa acggtcttgt aaaccacgca gttatgggag ccggcggaaa
     7801 agcaatcatg ttcaccagcc ttccggagta cttccgcaaa ctcttcatcg gaagtaacat
     7861 ctggcagacg atgggattca acgccatcat cttcatcgca gcactgaccg caatcagccc
     7921 ggaatattac gaagcagcaa cgatggatgg tgcttccaga tttcagaaaa tcctttacat
     7981 tgatttacct ctgattatgc cgaccatcat cctgatgctg atccttcagg taggtaacat
     8041 tatgaacgtg ggatatgaga aagcttatct gatgcagaac ggtaccaaca caaccgtcag
     8101 tgagatcatt tcgacatacg tatacaaagt aggtctccag accgcacagt acagttttgc
     8161 gacagccgtt ggcttgttta actccgttgt aaacttcatt attctggtta tcacaaacgc
     8221 agttgcaaag aagaccagcg atatcagtat tttctaagag tcagggggct tgaaaaatgg
     8281 caaataataa cactgtgaaa aagaaaaaag taaaaatgag tacgggagac aaggtcttta
     8341 ccgtgatcaa taccgtcatc atgatccttg tctgtattgc aattgtatat cctctgtatt
     8401 acgtgcttct ggcatctgtt acagacccgg ttgttgtaag ctccggtaaa attcttctct
     8461 atccggaagc gtggtacacc aacggatatg aaaccacact gaaataccag ccgctctgga
     8521 ccggctatgc aaacaccatc aaatataccg tattaggaac gctgatttcc ctggtctgca
     8581 ccattccggc cggctatgca ctttccagat acgatatggt aggaagaaaa ccgattatgt
     8641 tcctgttcac cttcacgatg ttcttcagcg gcggtatgat tccgatgtac ttaaccgtac
     8701 agcagctgca catctacaac acaacatggg ctatggtcct tccggtagca gtgtccgctt
     8761 acaacctgat cgtatgccgc tccttctttg aaagtggtat tccggtcgag cttctcgaag
     8821 cagcaaaaat cgacggctgc agcgattttg gattcttctt caaaattgca attccgctgt
     8881 cgaaaaccat catcgcagtt atgtgtctgt tctatgcgac tgcaatgtgg aaccagttct
     8941 tcaacgcgct gatgttcctg caggatgaca gcaagatgcc tcttcaggtc gttcttcgaa
     9001 acctggtact gatgaatcag tccaaccaga tgggaagtag tgcttctgag atggtaacaa
     9061 aacagaaact ggctgagcag ttaaaatact gtgtcgtagt tgtttccgca ttcccgcttc
     9121 tgtgcgtata tcctttcctg cagaaatact tcgcaaaagg tgttacgatc ggtgcagtca
     9181 agggctgatg cagaacagaa aatttattgt tgcagaattc at
//
LOCUS       DAOMUF010000075         9203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1893_length_9203_cov_3.095103, whole genome shotgun sequence.
ACCESSION   DAOMUF010000075 DAOMUF010000000
VERSION     DAOMUF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1893_length_9203_cov_3.095103"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..2187)
                     /locus_tag="PLN77_05780"
     CDS             complement(187..2187)
                     /locus_tag="PLN77_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43822.1"
                     /translation="MNNLLYARLAKTNLSKNRQNILPYLLSCIGTVVMFFIMDTLAQG
                     SGFDSMIGKDTILTVMGMGTYIIGLFAVIFLIYSNSFLAKRRKKEFGLFQILGMEKKH
                     LAKILFFESLYLWAASLGIGILLGVLLYRLLFLVLMKLTGLNGTIGFAFSAKAVGSTM
                     LLFGLTFVINFLLSLRQIHVSQPVELLHGGAQGEREPKTKLLTAVLGFMLLGVGYYLA
                     LTSQSSMDALDKFFNAIALVMVGTYCLFSAGSIAFLKILKKNKNYYYQTRHFTSISGM
                     LYRMKQNAVGLANICILSTGVLLVISISTCLWTGIEEVTYTRFPHQISIEANTGVSGI
                     MKTVEPVSQKMIEEVKTGIDQHLEEKQLRKKYESDQRYYVMLADVDRNKVEKTQSDDA
                     AASTLIKIMEESEYNQVTGEQVELEPGQALVFQEKQKNYGYDTIELGNQTYEVKKWNT
                     DKKSYVLDEKTQVYETMTVVTRNHEAKEAYAAVQGKEPEGYSWEYALDLIGDAGEQIT
                     VGDEIETILTESSFNGWVEVREKERNTVYSLYGSLLFLGVFVGTLFLMGAVMIIYYKQ
                     VSEGFDDRKRFQIMQKVGMSRKEIRQTIQSQVVTVFFMPLAVAVVHTMVAFPLTRRIM
                     AMLNFPDSNLFLAATAITIAAFAVVYLIVYVLTARAYYKIVE"
     gene            complement(2180..2947)
                     /locus_tag="PLN77_05785"
     CDS             complement(2180..2947)
                     /locus_tag="PLN77_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43823.1"
                     /translation="MAILEVEHLKKTYTTRLGSNRVEALKDVNFTVERGEFVAIMGES
                     GSGKTTLLNLLAALDKPTGGQVRLNGKNLGELKEKEIAGFRRENLGFVFQDFNLLDTF
                     SLRDNIYLPLVLEGKKYEEMSLRAEPIAKKLGITQILNKYPYEVSGGQKQRAAVARAL
                     ITNPELVLADEPSGALDSHAADGLMKLFGEINEEGQTIVMVTHSVKAACSAKRVLFIK
                     DGEVFHQLYRGSLGRDEMYQKISDTLTILATGGERDE"
     gene            complement(3094..4113)
                     /locus_tag="PLN77_05790"
     CDS             complement(3094..4113)
                     /locus_tag="PLN77_05790"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM43824.1"
                     /translation="MEQQKEGVRQEFMLLNWYLKEHLASAVRIAAFIGVFSLLCFLRN
                     VPQDVTVYMIVLMAAVGAAGFCFHYGKYRKHHLVLALLAAEPQGKTEALPEADGLLAR
                     DYQQIIASYERQFQEEQIKMEQKYRDMMEYYTMWTHQIKTPIAAMRLLLQEEDTPLSR
                     EMQSELFQTEQYVQMALQYLRMEKMTSDLVFARYDLDALIRQAVRKYAPLFIRRKIIL
                     SYEPVHCEVLTDEKWLVFVLEQILSNALKYTKSGSIHIYLSPDAPKTLVIEDTGIGIA
                     PEDLPRIFEKGYTGCNGRADKRSTGIGLYLCRQIMEKLSHTIRIESEMGVGTKVYLGL
                     DTVSL"
     gene            complement(4138..4809)
                     /locus_tag="PLN77_05795"
     CDS             complement(4138..4809)
                     /locus_tag="PLN77_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM43825.1"
                     /translation="MYRIMIVEDDEVIARAVQQSLVSWGMDAFCVQDFSGVLAEFAVK
                     NPQLVLMDIHLPFFNGFHWCQEIRKVSKVPVIFLFSASDNMNIIMAVNMGGDDFIAKP
                     FDLSVLTAKVQAMLRRTYEFAGETNFLEHRGAMLDTGSGVLNYRGKRIELTKNEWRIL
                     QSLLENKGKAVSRDMLMMHLWESDSFIDDNTLTVNVTRLRKKLEEAGLTDFIQTKKGV
                     GYLIA"
     gene            complement(4945..5466)
                     /locus_tag="PLN77_05800"
     CDS             complement(4945..5466)
                     /locus_tag="PLN77_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRM43826.1"
                     /translation="MNNEVLNAIKTRRSIRKYKAEQITDEQLDAILEAGTYAATGMGK
                     QSPVIVVVQKPELIAKLSKMNAAVMGTTSDPFYGAPTVLIVLADPERGTYVEDGSLVM
                     GNLMLAAHAVGVDSCWIHRAKEEFASPEGKALLKEWGLSENYVGIGHCILGYRDCEYP
                     EAKERKADYIVRV"
     gene            complement(5545..5721)
                     /locus_tag="PLN77_05805"
     CDS             complement(5545..5721)
                     /locus_tag="PLN77_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6472 family protein"
                     /protein_id="HRM43827.1"
                     /translation="MATSCESCGNFVYDEDYECYTCEVDLDEDEMARFLQDKFYDCPY
                     YQLGDEYRIVRKQM"
     gene            complement(5837..7039)
                     /locus_tag="PLN77_05810"
     CDS             complement(5837..7039)
                     /locus_tag="PLN77_05810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRM43828.1"
                     /translation="MSNAVVTLKKGEGRALKQGGAWIFDNEIASILGAYENGDIVLVR
                     DFDGYPMGRGFINEHSKIRIRMMTRNIDQEIDEAFLRKRVQDAWEYRKKVTDTSSCRL
                     IFGEADFLPGLVVDKFSDVLVVQSLALGIDKMKMTIIRLLEEVLAADGVKIRGVFERS
                     DAKVREQEGMERVKGFLSEPFDTSVEIVENGVHYLVDVKDGQKTGFFLDQKYNRLAVQ
                     KLCKDARVLDCFTHTGSFALNAAYAGAKEVIGVDASELGVEQARKNAALNGLENVASF
                     VCEDVFELLPRLEKEGEKFDVVILDPPAFTKSRNSIKNAIKGYREINLRGMKLVKDGG
                     YLCTCSCSHFMDYELFTKTINQAAHNVHKRLRQVEYRTQAPDHPILWAADESYYLKFY
                     IFQVVDEK"
     gene            complement(7176..8084)
                     /locus_tag="PLN77_05815"
     CDS             complement(7176..8084)
                     /locus_tag="PLN77_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM43829.1"
                     /translation="MQDLELEMSRCRVCPRKCGADREHGKTGYCKAPSGVAAARAALH
                     FWEEPCISGTTGSGTVFFSGCNLRCVYCQNHHIAEMESGKVISIGRLSDIFLELQAQN
                     ANNINLVTPSHYVLQIREALFQAKKKGLTVPVVYNCGGYESVEALQALDGLIDIYLTD
                     FKYMDSSLAKVYSRAEDYPETAKRALAEMVRQTGEAVFDENGLMKKGVIVRHLLLPGA
                     VKNAKAVVKYVYETYGDTVWLSLMSQYTPLPQVENISPLNRKTTKREYERLLDYTFSL
                     GVTNAFLQEGPVAEESFIPEFDGRGI"
     gene            complement(8174..9049)
                     /locus_tag="PLN77_05820"
     CDS             complement(8174..9049)
                     /locus_tag="PLN77_05820"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HRM43830.1"
                     /translation="MLKKIGRNDPCWCGSGKKYKDCHLALDEKINSYKLKGWKIPPKK
                     IIKRPDQIDGIRESGLVNIAVLDYVAENIHAGMSTEDIDRLVAEKTKELGGIAAPLNY
                     EGFPKSVCTSINDAVCHGIPNEEEILQDGDIINVDVSTIYKGYFSDSSRMFCIGEVDD
                     VSKKLVEDTLKAVQLGLSEVRPFNRLGNVGAVINEFAQSQGYKVVRDIGGHGVGLEFH
                     EDPFVSYVTRRDTGMLLVPGMVFTIEPMINLGTDEIFIDSDNDWTVYTEDGAPSAQWE
                     VTVAVTEDGYEILAY"
BASE COUNT         2196 a         2582 c         1923 g         2502 t
ORIGIN      
        1 atggaaaaga cgaactcaat ggaaaggaag ctcttttcaa agttaccgtc aacggcattt
       61 ataaatagaa caaccaaaaa gacatccccc gcagattttc cgtctgcgcc ggatgtcttt
      121 ttcttattat ttttttgtta agtttttttc aaagacgctt cgatgtatta aaaaaagtac
      181 atcttcttat tccacaattt tgtaatacgc ccgcgccgtc agcacataga caatcaggta
      241 aaccacggca aacgccgcaa tcgtaatcgc tgttgcagcc aggaacagat tgctgtccgg
      301 gaaattcagc attgccatga tacgcctggt caatggaaac gctaccattg tatgcaccac
      361 ggccaccgcg agcggcatga aaaagaccgt taccacctgt gactgaatcg tctgacggat
      421 ttctttgcgg ctcatgccga ccttctgcat aatctggaac cgttttcggt catcgaagcc
      481 ttctgaaacc tgcttgtaat agataatcat aacggcaccc atcaaaaaca gcgtaccgac
      541 gaaaacacct aaaaacagaa gtgaaccata cagactgtaa accgtattgc gctctttttc
      601 gcgcacttcc acccatccgt taaaagatga ctccgtcagt atcgtctcaa tctcatctcc
      661 aacagtgatc tgttcgccgg catccccgat aagatccaga gcatattccc aactgtatcc
      721 ttctggctcc tttccctgca cagctgcata cgcttccttt gcttcatggt tccgcgtcac
      781 caccgtcatt gtctcataca cctgcgtctt ctcatccaga acgtacgatt tcttatcggt
      841 attccatttt tttacttcat acgtctgatt tcccagttca atcgtgtcat atccataatt
      901 tttctgtttt tcctgaaaaa ccagagcctg ccctggttcc agctctacct gttcgccggt
      961 tacctgattg tactccgact cttccatgat ttttatcagt gtgctcgccg cagcatcatc
     1021 tgactgtgtt ttttctactt tgtttctatc tacatcagca agcataacat agtatctctg
     1081 atcactttca tacttttttc tgagctgctt ttcttccaga tgctgatcaa tcccagtttt
     1141 cacttcctct atcatcttct gactgaccgg ttccaccgtc ttcataatgc cgctcacacc
     1201 agtatttgct tcgatcgaaa tctgatgtgg aaaccgcgta taggtaacct cctcaatccc
     1261 cgtccacaaa catgtgctga tggagataac cagaagcaca cccgttgaca gaatacagat
     1321 attcgcaaga ccgaccgcat tttgcttcat gcggtacagc atgcccgaaa tgcttgtaaa
     1381 atggcgtgtc tggtaatagt aattcttgtt tttcttcaga attttcaaaa atgcaatact
     1441 tccagcagaa aacaggcagt acgttcccac cataaccaag gcgattgcat taaaaaactt
     1501 atcaagtgcg tccatcgagg attggctggt cagagccaga taatagccaa caccaagaag
     1561 catgaatcca agaaccgccg tcaaaagctt cgttttcggt tcccgctcac cctgcgcgcc
     1621 gccgtgcagc agttcgaccg gctgtgagac gtggatctgg cgaaggctta agagaaaatt
     1681 gatcacaaaa gtcaggccga acagcagcat cgtacttccc acggcttttg cactgaacgc
     1741 aaaaccaatt gttccgttca gtccggtcag cttcatcagc accagaaaca gcaggcggta
     1801 cagcaacaca ccgagcagaa taccgattcc aagcgatgcc gcccacagat acagactctc
     1861 aaaaaagagg atcttcgcca gatgcttttt ctccatgccg agaatctgga acagaccgaa
     1921 ctcttttttt cttctttttg ccagaaaact gttgctgtag atcagaaaaa tcaccgcgaa
     1981 caggccaatg atataggttc ccatccccat cactgtcagg atcgtatctt ttccgatcat
     2041 ggaatcaaag ccacttccct gcgccagtgt atccatgata aaaaacatca ccaccgtgcc
     2101 gatgcatgac agcagatacg gaagaatatt ctgacggttt ttgctcaaat ttgtttttgc
     2161 cagtctggca tacagcagat tattcatccc gttcaccacc cgtcgcaaga attgtcagcg
     2221 tatcggagat cttctgatac atctcatcgc gccctaaaga accgcgatac agctggtgga
     2281 agacctcgcc gtccttgatg aacagcacac gcttcgccga gcaggccgcc ttcacactgt
     2341 gcgttaccat aacaatcgtc tggccctcct cgttgatctc cccgaacagt ttcatgaggc
     2401 catccgctgc atgggaatcg agcgcgccgg aaggctcatc cgcaagaacc agctccggat
     2461 tggtaatcag cgcacgcgca acggctgcac gctgcttctg tccgccggac acctcatatg
     2521 ggtatttatt gaggatctgg gtgatgccga gttttttcgc aatcggctct gcccgaagac
     2581 tcatctcctc gtattttttt ccttccagca cgagcggcag ataaatgttg tcacgcaggg
     2641 aaaaggtatc gagcaggttg aagtcctgga acacgaaacc taagttttcg cggcggaatc
     2701 cggcaatctc cttctctttt aattcgccga gattttttcc atttaaccgc acctgtccgc
     2761 cggttggctt gtctagtgct gcgagcaggt tcagaagcgt cgtttttccg cttcccgatt
     2821 cgcccatgat tgcaacaaat tcgccccgtt ccactgtaaa attaacgtct ttcaacgctt
     2881 ccacccggtt gctgccaagg cgcgtggtat atgttttttt cagatgttct acttccagaa
     2941 ttgccatgtc acattttcct ttctgtctga actgttttct taattgacaa ggcttattat
     3001 acccgtccgc gccctcttct gccttaggat catcttacaa aaagaccggc aaccttacat
     3061 ttctgtaaga ttcccggcga agctctctat gtactacagg gatacggtat ccagcccaag
     3121 atacaccttc gttcccactc ccatctccga ctcgatgcgg atggtatgcg aaagtttctc
     3181 catgatctgg cggcacagat acagtccgat gcccgttgac cgcttgtctg cgcggccgtt
     3241 gcagcccgtg tagccttttt cgaaaatccg cggcagatcc tccggcgcaa tgccgatgcc
     3301 ggtgtcctca atcaccagcg tttttggtgc atccggtgac agatagatgt gaatgctgcc
     3361 acttttcgtg tatttcagcg catttgacag aatctgctcc agcacaaaga cgagccattt
     3421 ttcatctgtc agcacctcgc agtgcaccgg ctcgtaggaa aggataatct tccggcggat
     3481 aaaaagcggg gcatatttcc gcacagcctg gcggatcagc gcatccaaat cgtaccttgc
     3541 aaagacaaga tccgatgtca ttttttccat gcgcaggtac tgcagcgcca tctgcacata
     3601 ctgctccgtc tggaaaagtt cgctctgcat ttcacgcgaa agcggcgtat cctcctcctg
     3661 cagaagcagc cgcatcgccg caatcggcgt tttgatctga tgggtccaca tcgtgtaata
     3721 ctccatcata tcgcggtatt tctgctccat cttaatctgt tcttcctgaa actgccgctc
     3781 gtaggatgcg atgatctgct ggtaatcccg cgccagaagc ccgtccgcct ccggaagcgc
     3841 ttctgttttg ccctgcggtt cggcggcaag gagtgctaag accagatgat gtttccggta
     3901 cttcccataa tgaaagcaaa aacctgcggc tccgacagca gccatcagca cgatcatata
     3961 aacggtgaca tcctgcggca cattgcgcag aaaacagagg agtgaaaata ctcctataaa
     4021 agcggcaatc cgcacggccg atgcaagatg ctctttcaaa taccagttaa gaagcataaa
     4081 ctcctgccgg actccctctt tctgttgttc catccttttt ctcctgttct gcttccgtca
     4141 tgcgatcagg tagccgacac cctttttcgt ctggataaag tctgtaagtc ctgcctcctc
     4201 cagttttttc cgcaggcgcg tgacgttgac ggtcagcgtg ttgtcatcta taaagctgtc
     4261 cgactcccac agatgcatca tcagcatatc gcggctgacg gcttttcctt tattttcgag
     4321 aagggactgc agaatgcgcc actcattttt tgtcagctca atccgctttc cccggtaatt
     4381 cagcacacca cttcccgtat cgagcatggc accgcggtgc tcgaggaaat tcgtttctcc
     4441 ggcaaattcg taggtgcggc ggagcatcgc ctggactttg gcggtcagga cacttaaatc
     4501 aaacggcttc gcgataaagt catcgccgcc catattaacg gccataatga tgttcatgtt
     4561 gtccgatgca gaaaaaagaa aaattaccgg cactttagag acttttcgaa tctcctggca
     4621 ccagtgaaat ccgttaaaaa agggaagatg aatgtccatc agcactaatt gcggattttt
     4681 cacggcaaat tcggcgagaa cgccggaaaa atcctgtacg cagaacgcat ccatgcccca
     4741 ggaaaccagg ctctgctgca cggcgcgggc gatgacttca tcatcttcta caatcataat
     4801 tcgatacatg gctgttcctc tgttgtctct agcaaagcgg atattcgcaa tccgcttcgc
     4861 tacttgcttg attcggttaa aaatatggac tccgggcggg tgttttcaac gcacagccgc
     4921 tcccggagcc gttttcgcag atgattatac tcttacaata taatctgctt ttctttcttt
     4981 tgcctccggg tactcacagt cgcgatagcc gaggatgcag tggccgatgc caacataatt
     5041 ttcggaaagt ccccactctt ttaagagggc ttttccctcc gggctcgcga actcttcttt
     5101 tgcacgatgg atccagcagg aatccacacc aacggcatgt gctgccagca tcagatttcc
     5161 catgacaaga gagccgtcct ccacataagt tccgcgctcc gggtcagcaa gcacgatcag
     5221 aacggtcgga gcaccgtaga acggatcgga tgttgtaccc ataacagccg cattcatttt
     5281 cgaaagcttt gcaatcagct ccggtttctg taccacaaca atcaccggcg actgttttcc
     5341 catgccggtt gccgcatacg ttccagcctc cagaattgca tcgagctgct catcggtaat
     5401 ctgctccgct ttgtatttgc ggatgcttct tcttgttttg attgcattta atacttcgtt
     5461 gttcatggta aaaatcctcc tgttctttta tatatgtact ttatcgcaaa cgccgcagct
     5521 ttgtataccg gacgcattgc ctgcctacat ctgtttccgc acaatccgat actcgtcgcc
     5581 gagctggtag tacggacagt cgtaaaattt atcctgcaga aaacgtgcca tctcatcctc
     5641 atcgagatcg acctcgcagg tgtagcactc ataatcctca tcatacacaa aattgccgca
     5701 cgactcacag cttgtggcca tctggcggga gtgctttttc ttttgtgcat ggggtgttgg
     5761 ttctgtcata tggcaattcc tcccatttct tacacttcga ttttccgttt cggcgcttcc
     5821 ggcagaggaa gaatttctat ttttcatcca caacctggaa aatataaaac ttcaggtaat
     5881 aggactcatc cgccgcccac agaattgggt gatccggagc ctgggtgcgg tattctacct
     5941 gacgaaggcg tttgtggacg ttgtgagccg cctggttgat ggttttcgtg aagagttcgt
     6001 agtccataaa gtgggaacac gaacaggtgc acagatagcc gccgtctttg accagtttca
     6061 tgccgcgcag attgatttca cgatatccct tgattgcgtt tttgatggag tttcttgatt
     6121 ttgtgaaggc cggcggatca aggataacaa catcgaattt ttcaccttct ttttcaagac
     6181 gcggcaacag ttcaaagaca tcctcgcaca cgaaagatgc tacgttttcc agaccattta
     6241 aggctgcgtt tttccgtgcc tgctctacac cgagctctga tgcatcaacg ccgataactt
     6301 cctttgctcc tgcatatgcc gcattcagtg caaacgatcc ggtatgggta aagcagtcga
     6361 gcactctcgc gtctttgcag agcttctgca cagccagtct gttgtacttc tggtcgagga
     6421 aaaatcctgt tttctggccg tccttgacat cgacgagata atgaacacca ttctcgacaa
     6481 tttccacgct ggtgtcaaac ggctcggaga gaaatccttt cacacgctcc atgccctcct
     6541 gctcacgcac ttttgcatcg cttcgctcaa acacgccgcg gatcttaacc ccgtcagctg
     6601 ccagaacctc ctccaacagg cggataatcg tcattttcat tttgtcgata ccaagtgcca
     6661 gcgactgaac gaccagcaca tcggagaatt tatccactac gagtcccggc aggaagtctg
     6721 cctcgccaaa gatcaggcgg cagcttgatg tgtctgtcac ttttttgcgg tattcccagg
     6781 catcctgcac tctttttctc aaaaatgctt catcgatttc ctggtcgatg tttcttgtca
     6841 tcatacggat acggattttg gaatgctcgt tgatgaaacc gcgccccatc gggtatccgt
     6901 cgaagtcgcg caccagcacg atatctccgt tttcatatgc tcctaaaatc gaagcgatct
     6961 cattatcaaa aatccaggct ccgccctgct ttagggcgcg gccctctcct tttttcagtg
     7021 ttacaactgc gttgctcaaa agtttttcct ccttaaaaca atatagttag ccatatctaa
     7081 tcatagacaa atgtgttttg cttgcattct caaaaccgcg cttccagcgc tttttgctgc
     7141 tgcgcagctc ctttttgcac gagtgaacta taacgtcaga ttcctcttcc gtcaaactcc
     7201 ggaatgaagc tctcctcagc caccgggcct tcctgtaaaa aggcgttggt gacgccgagg
     7261 gaaaacgtat agtccagcag acgctcatac tcccgctttg tcgttttccg gttcagcgga
     7321 cttatgtttt ccacctgcgg aagcggcgtg tactggctca tgaggctcag ccagacggta
     7381 tcgccgtacg tctcatagac gtattttacc acagcttttg cattttttac agcaccgggc
     7441 agcaataaat ggcgcacaat cacacctttt ttcatcagac cattctcatc gaatacagcc
     7501 tctcctgtct gacgcaccat ttccgccagc gcccgttttg ctgtttccgg gtaatcttct
     7561 gcccgcgagt acacttttgc aagggaggag tccatatact taaaatctgt cagataaata
     7621 tcgatcagcc catccagggc ctgcagggct tccacgctct catacccgcc gcagttatac
     7681 acaaccggca cggtaagtcc ctttttcttt gcctgaaaaa gtgcctcccg gatctgcagc
     7741 acgtagtggc ttggtgtcac aagattgata ttgtttgcat tctgtgcctg cagctccaga
     7801 aaaatgtccg aaagccgccc gatcgaaatc acttttccgc tctccatctc cgcaatatga
     7861 tggttctgac agtagacaca ccgcagattg cacccggaaa agaaaaccgt ccctgacccc
     7921 gtcgtcccgg aaatacaagg ctcctcccag aaatgcagcg ccgcccgcgc cgccgcaacc
     7981 ccggagggcg ccttacaata tcccgtcttt ccatgctccc ggtccgctcc gcacttccgc
     8041 ggacaaaccc ggcaccggct catctccagt tccaaatcct gcatcgtatt tctccctttt
     8101 ctctttagaa aaaagccgtg tctccggaaa atagttcttc cacagacaca gcttttgtat
     8161 ttctatgcgt caattaatat gccagaattt catatccatc ttctgttacc gcaaccgtaa
     8221 cctcccactg ggctgacggt gcgccgtctt cggtgtagac agtccagtcg ttgtcggagt
     8281 cgatgaagat ctcatccgtt ccgagattga tcatcggctc aatagtgaat accatacccg
     8341 gtacgagcag cattccggta tctcgtcttg tgacatagct gacgaacgga tcctcgtgga
     8401 actccagacc gacgccatga ccaccgatgt cacggacaac tttatagccc tggctctggg
     8461 caaattcatt gattaccgcg ccgacattgc caagccggtt aaacggacgt acttctgaga
     8521 gtccaagctg tacggctttt aacgtatctt ccaccagctt ctttgaaaca tcgtctacct
     8581 caccgatgca aaacatacgg gaagaatcag aaaaatatcc cttataaata gtagaaacat
     8641 cgacgttgat gatgtctccg tcctgcagaa tttcctcctc attcggaatt ccatggcata
     8701 ccgcatcgtt gatggaggta cacacgctct tcggaaatcc ttcataattc agcggtgctg
     8761 caattccgcc gagttctttt gtcttttctg cgaccagacg gtcgatatct tccgtactca
     8821 tgcctgcatg aatgttttcg gccacataat ccagaactgc aatattgaca agaccgcttt
     8881 cacgaattcc atcgatctga tcaggtcttt tgataatttt cttcggcgga attttccatc
     8941 cctttaattt ataagaattg attttctcat ccagcgccag atggcagtct ttgtacttct
     9001 tcccgctgcc gcaccagcag ggatcatttc ttccaatttt tttcagcata atgttctatc
     9061 ctctttctga acgcggaacg acagatccga ttttctgtcc cccgctgaaa ttcatataac
     9121 tcctctacta ctttaccaca ctccggcaaa actgcaagca aaagttcaca aatgaataac
     9181 tctctcaaaa gagaccatct gcc
//
LOCUS       DAOMUF010000076         9167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1911_length_9167_cov_2.920874, whole genome shotgun sequence.
ACCESSION   DAOMUF010000076 DAOMUF010000000
VERSION     DAOMUF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1911_length_9167_cov_2.920874"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..821
                     /locus_tag="PLN77_05825"
                     /pseudo
     CDS             43..821
                     /locus_tag="PLN77_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     assembly_gap    822..831
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            845..1273
                     /locus_tag="PLN77_05830"
     CDS             845..1273
                     /locus_tag="PLN77_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRM43831.1"
                     /translation="MESRGFYNEILTEHNLRPEFKHDLPDADIVLEGVNPSCGDDIWL
                     KLKVDGDVITDGAFVGDGCAISQASADIMLGMIIGKTKDEALRLGNLFLRMIKGEASD
                     EEIDELEEAGALKDISHMPARVKCAVLGWHTLEEAFKQEK"
     gene            1471..2772
                     /gene="eno"
                     /locus_tag="PLN77_05835"
     CDS             1471..2772
                     /gene="eno"
                     /locus_tag="PLN77_05835"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRM43832.1"
                     /translation="MKSLKIEEVKGREILDSRGNPTVEAEVVLAGGVVGRGMAPSGAS
                     TGEFEALELRDGDKARYLGKGVQKAVENINTVINDTLKGMDASDTYAVDKAMIDADGT
                     KDKSRLGANAILAVSIACARAAANALEIPLYRFFGGVSGNRLPVPMMNIINGGCHALS
                     SGLDVQEFMIMPVGASSFKECLRWCAEVFHALAAILKERGLATSVGDEGGFAPALKSD
                     EEAIETILEAVKKAGYEPGRDFKIAMDAASSEWKTGTKGEYKLPKAGTVFTSKELIVH
                     WKALVEKYPIISIEDALDEEDWEGWKELTAELGGKVQLVGDDLFVTNTERLAKGISLG
                     CGNSILIKLNQIGSVSETLEAIKMAHKAGYTAISSHRSGETEDTTIADLAVALNTCQI
                     KTGAPSRTERVAKYNQLLRIEEELGTAAVYPGARAFNVTQD"
     gene            2979..3653
                     /locus_tag="PLN77_05840"
     CDS             2979..3653
                     /locus_tag="PLN77_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM43833.1"
                     /translation="MSERLWNILLDSFWKIFLPGLKVTIPLTVISFSIALVIATAMAM
                     VQFANIKGLRQIARFYIWIFRGTPLLVQLFVVFYGLPRAGILIDPFPAAVLVFSLNEG
                     AYSAETMRAALEAVPAGQLEAGYCVGMSYLQIMRRIILPQAMRTAFPPLSNSLIAMVK
                     DTSLAANITVTEMFMVTQRIVARTHEPLALYIEVGLIYLLFSTVLTKLQGIGEKKLST
                     YQKKGE"
     gene            3657..4400
                     /locus_tag="PLN77_05845"
     CDS             3657..4400
                     /locus_tag="PLN77_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM43834.1"
                     /translation="MLEIKGLKKAFGSLEVLKGVDLKVNQGDVVAILGPSGSGKTTML
                     RCVNFLEHADGGSLIFDGKEYPFHNISKKDIAAIRQKTAFVFQNYNLFLNKTALHNVT
                     EGLIIGRKMPKTRAEEKARAALEKVGMLDRADYYPHQLSGGQQQRVAIARALATDPEI
                     IYFDEPTSALDPELTGEVLGVMRQLAKEGMTMLVVTHEMGFARNVSNKVVFMENGVVV
                     EEGPSKEFFANPREERTRTFLQNIEQHYE"
     gene            4523..5380
                     /locus_tag="PLN77_05850"
     CDS             4523..5380
                     /locus_tag="PLN77_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM43835.1"
                     /translation="MKKKMAAVFLAGILTSSFLLTGCGNSTADNSSASSSSSASSASA
                     SGDLLEQIQSKGEIVVAMEGTWAPWTYHDEDDNLVGYDVEVAQQIAEKLGVKATFVEG
                     EWDGLLAGIDSGRYDIMVNGVDITEERAEKYSFSDPYAYNRTAVIVNSANDEIQSMED
                     LDGKSTANTISSTYAEVAEKYGAAVTGVDDLEQTFELLLSGRIDATLNAEVTYYDYMK
                     AHPDAEIKIACLDPESTKVGIPMKKGDETKTLLEAVNKALGELAEDGTLTELSEKYFG
                     TDISKEAAE"
     gene            5408..6181
                     /locus_tag="PLN77_05855"
     CDS             5408..6181
                     /locus_tag="PLN77_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRM43836.1"
                     /translation="MIANYHTHTWRCRHADGTEREYVERAIEGGLKILGFSDHSPYPF
                     PDGYDSGMRMRLDQVEGYVDTVLALKKEYDKDIEIHLGLETEYFPRFWDQLIDFLSDY
                     PFEYFVLGQHSLGNEIEKILYSGHGTTDGSYLKQYVDQCLAGIDTGKYLYLAHPDLFL
                     FNGDEAVFDREYRRLCREVKKRNIPLEINFLGLMEERWYPNEKFWKIAGEEGNTAVFG
                     CDAHTPKAVWNPETEQKALQLAAKYGLKVQETVPMPKTF"
     gene            complement(6264..7649)
                     /locus_tag="PLN77_05860"
     CDS             complement(6264..7649)
                     /locus_tag="PLN77_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM43837.1"
                     /translation="MKTVNHLGTDPIGKLVPRIAFPSMLAQFVSVLYSIVDRMYIGHI
                     ASVGDLALAGVGVCGPILTMIGAFSSLIGVGGAPLLGIRLGEKNEKEASRILANAFLM
                     LVSLSLVLTAAALFFTRPMLMAFGASTVTYPYARAYFMIYVSGSVFALLSTGLNQFVI
                     CQGFASEGMKSVLLGAVLNLLLDPVFIFVLHLGVRGAAIATVLSQIASCLYVLHILFG
                     KTIPIRITFGGYSFAIMKRILTVGFTPFLIIAIDNVMIIAMNAVLQRYAPAGQGDSFI
                     TCATIVQSFMLIITMPLGGISGGTQSILAYNYGAGNSRRILEAQKTIIALCAGFTTLM
                     FLVSNAAGTLFAQLFTSDPQIVSMVVRAIHISTLSVIPLGIQYEIVDGFTAMGCVRYS
                     FALSFFRKFVYFTALFLLPRFFALENIFYVEPISDLIGPVVSMIVYWTSIQKILKQRE
                     EAVRLMHTKKA"
     gene            complement(7779..8258)
                     /locus_tag="PLN77_05865"
     CDS             complement(7779..8258)
                     /locus_tag="PLN77_05865"
                     /inference="COORDINATES: protein motif:HMM:NF040112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HRM43838.1"
                     /translation="MNKRTMKKMAKAKQQAAEAAKTAETVVKTAAPAKEVKPTTEKTL
                     HTEAAEGKAEAAKEPKKATKKETVKKTVKKETTETMAEATKTPRKMTKVSDTVLEIFE
                     TKVSMKDLEAVIKKEVKANGLKGEIKIYLNAEQRAAYYTVDGQGSDDFKVDLTTLAE"
     gene            complement(8344..>9167)
                     /locus_tag="PLN77_05870"
     CDS             complement(8344..>9167)
                     /locus_tag="PLN77_05870"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM43839.1"
                     /translation="CCPKKTVPAMRGLLKVRRKLPPLFAVPTTAGTGSEATAAAVITD
                     CIDGKPYKYAVNDLCLIPDTAVLDPRLTISLPSSMTADTGMDALTHAMEAFLNLFASR
                     SVQNASIEAVCENFHALPEAWRDGTHLAARQEMLHASYLAGFAFTNNFVGYVHAIAHA
                     VGALYHIPHGRANAVLLPAVLREYGSCIAPKLAVLADALSLGGDTVSEKAERMIAAIE
                     AMRDSFDIPSTLPHLSPNDYTEIARRALKEANPTYPVPQIWDSEKIFSVLRRVQS"
BASE COUNT         2476 a         2109 c         2477 g         2095 t
ORIGIN      
        1 atatataaat ccgattgaaa tactcggaaa aaaggtctac aatcggaggc gaaaatccat
       61 tatctggaat gtaccagtga gggcgagctg aacgatgagg agctccaaaa gggcattaat
      121 gagagaacac gtcttgtggc tgttgcgcag gtatcgaatg tgctcggctg cgtgaatccg
      181 ctccagaaaa tcacggagat ggcgcaccgc gtgggagccg ttgtcgttgt ggacggcgcc
      241 cagagcgttc cgcatatgcc ggtcgacgta caggcgctgg atgcagattt tctcgccttt
      301 tccggccata agctgatggg cccgatggga atcggctgtc tgtatggaaa actggaactg
      361 ctggaaaaaa tgccgccgtt tttaaccgga ggcgagatga tcgattccgt tcaccgcgac
      421 ggagccgttt ttgcaccggt tccgcagaaa tttgaggcgg gaacggtaaa tgcagccggt
      481 gcggtggggc tggccgcagc gatccggtat ctgcaggaaa aaggatttga ttatatccag
      541 aaaaaagagc tggaactgac gcaacgtgct atggaaggac tcggcgcgct gccgtacgtt
      601 catatcctcg gttccaaaga tccggccaac cacacgggca tcgtttcgtt tacgattgac
      661 ggcgtgcatc cgcacgatgt gagcgaaatt ctttcttctg acggcatcga tgtgcgggca
      721 ggccaccact gtgcacagcc gcttctggaa tatcttggcg tccgaagttc gacaagagcc
      781 agcatgatgt tttataatac accggaagag attgatgcac tnnnnnnnnn naggagaatg
      841 ggctatggag agtagaggat tttataacga aattttgacg gagcacaatc tgcgtccgga
      901 attcaagcat gatctgccgg atgcggacat cgtactcgag ggcgtcaacc caagctgcgg
      961 cgatgatatc tggctgaaat taaaagtcga cggcgatgtg atcacggacg gtgcgttcgt
     1021 gggcgacggc tgtgcgattt cgcaggcatc ggcggacatt atgctcggca tgatcatcgg
     1081 aaagacgaag gacgaggcac tgcgcctggg aaatctgttt ctgcgcatga tcaaaggaga
     1141 agcatccgac gaggagattg acgaactcga ggaggctggt gcactgaaag atatctccca
     1201 catgccggca agagtgaaat gtgcagtgct tggatggcat acgctggaag aggcatttaa
     1261 acaggaaaaa taagaacaga aaaacttttt gcatttctga gaaaaagtat cactttaccc
     1321 acttgcaatt ttcaaaaaat tagtgataat gaatcatagt tagtaaagac taatcaaagc
     1381 gaatcaatca ttgttatctc ccgtctgagg atcctggaca cttcgcaggc gggaaactcc
     1441 agaaacaagc gagaaaggag cacacgaaaa gtgaagagcc tgaaaattga ggaagtaaaa
     1501 ggaagagaaa ttctggattc cagaggaaat ccaaccgtag aagctgaggt tgttcttgca
     1561 ggaggcgttg taggacgcgg catggcacca agcggcgcat ctaccggtga gtttgaggca
     1621 ctggaattac gagatggaga taaagcaaga taccttggaa aaggtgtgca gaaagcagta
     1681 gaaaacatca acaccgtcat caacgatacc ttaaaaggta tggatgcatc cgatacctac
     1741 gctgtagata aagctatgat cgatgcggac ggaacaaagg ataaatcccg cctgggtgca
     1801 aatgccattc tggctgtatc cattgcctgc gcaagagcag cagcaaacgc acttgagatt
     1861 ccgctgtacc gcttcttcgg cggcgtttcc ggaaatcgtc tgccggttcc gatgatgaac
     1921 atcatcaacg gcggatgcca cgcactttct tccggcctgg atgtacagga gttcatgatc
     1981 atgccggttg gagcatcttc tttcaaagag tgcttaagat ggtgcgctga ggtcttccat
     2041 gcactggctg ccatcttaaa agagcgcgga ctggcaacat cggttggaga tgaaggcggt
     2101 tttgcaccgg ctctgaaatc cgatgaagaa gccatcgaga ccattcttga ggcagtgaaa
     2161 aaagcaggct atgagccagg cagagacttc aagatcgcga tggatgctgc ttcctccgag
     2221 tggaagacag gaaccaaggg tgaatacaaa ctgccgaaag ccggcactgt atttacttcc
     2281 aaagagctga tcgttcactg gaaagcactg gtagagaaat acccgatcat ctccatcgag
     2341 gatgctctgg atgaagaaga ctgggaaggc tggaaagagc tgaccgcaga gcttggcgga
     2401 aaagtacagc ttgtcggtga cgacctgttt gtaaccaata ctgagcgtct ggcaaaaggt
     2461 atctcccttg gctgcggaaa ttccatcctg atcaaactga accagatcgg ttctgtatct
     2521 gagacccttg aggcaatcaa gatggctcac aaagcaggct acaccgcaat ttcctcccac
     2581 cgttccggtg agaccgagga taccaccatc gcagacctgg cggttgccct gaacacctgc
     2641 cagatcaaga ccggtgcacc gagccgtacc gagcgtgttg caaaatacaa ccagcttctc
     2701 cgaatcgaag aagaacttgg aacggccgct gtataccctg gtgcaagagc atttaatgtg
     2761 acacaggact aagcagtaaa taacataata ccataaaaaa agacaagatc cttcctctta
     2821 aaaccaacca taaataagag tccccggggc cgttgaaaaa cgtgtttccc ggggattctt
     2881 gcatgcgggg gaggggtata tgaaaaaaca cttgacttga agcggacttg aagtgttatc
     2941 ctatctagga aaatgtaaaa taagggggat atatcgaaat gagcgaaaga ttatggaata
     3001 ttcttctgga ttctttctgg aaaatctttc tgccgggcct taaggtgacg attccgctta
     3061 ctgtaatctc gttttccatt gcacttgtaa ttgcgacggc aatggctatg gtacagtttg
     3121 cgaacatcaa aggccttcgt cagattgcac gtttctatat ctggattttc cgcggaaccc
     3181 cgcttctggt ccagcttttc gttgtgtttt acggacttcc gcgcgccggt attctgatcg
     3241 atccgtttcc ggcggcagtt ctcgtctttt ccctgaatga gggcgcctac agtgccgaga
     3301 cgatgcgtgc ggccctggaa gccgttccgg caggccagct cgaagccggc tactgtgtcg
     3361 gcatgtcgta tctgcagatc atgcggagaa ttatccttcc gcaggcgatg cgcaccgctt
     3421 tcccgccgct ttccaactct ctgatcgcca tggtaaagga tacttccctt gccgcaaata
     3481 tcaccgtaac ggagatgttt atggtaacgc agcggattgt agcgcgcacc catgagccgc
     3541 tggcactgta tattgaagta ggactgatct acctgctgtt ctccaccgtg ctcacgaagc
     3601 tgcagggaat cggagagaag aaactgagta catatcagaa aaaaggggaa taagccatgc
     3661 tggaaatcaa aggattaaag aaagcgttcg gcagcctgga agtattaaaa ggtgtagatt
     3721 taaaagtaaa ccagggcgat gttgtcgcaa tcctcggccc gagcggttcg ggaaaaacaa
     3781 ccatgctgcg ctgtgtcaac tttctggagc atgcggacgg cggcagcctg atttttgacg
     3841 ggaaagaata tccgttccac aacatcagca aaaaagatat cgcggcgatc cgtcagaaaa
     3901 ccgcatttgt atttcagaac tataacctgt tcctgaacaa aacagccctt cataacgtaa
     3961 ccgagggcct gatcatcggc agaaaaatgc caaaaaccag ggcggaggaa aaagcgcgtg
     4021 cagcgcttga aaaagtaggc atgctggacc gtgcggacta ctatccgcat cagctttccg
     4081 gcggacagca gcagagggtg gcaatcgccc gtgcactggc aaccgatccg gaaattatct
     4141 attttgatga gccgacctca gcactcgacc cggagctgac cggcgaggtg cttggtgtta
     4201 tgcgccagct tgccaaagag ggaatgacga tgctcgttgt cacgcatgag atgggctttg
     4261 caagaaacgt ttcgaacaaa gttgtcttta tggaaaacgg cgttgtcgtt gaggaaggtc
     4321 cgtcgaagga atttttcgca aatccgcggg aagagcggac gagaacattt ttgcagaata
     4381 ttgaacagca ttacgagtaa gatatccagc aaagcggcaa atcctgtccg gagcacggaa
     4441 tccggacagt ttgcaataaa gaaaacagaa gaagcaaagg cagcttcttc cagaacaaaa
     4501 caaaaaagga gtggcgaata ttatgaaaaa gaaaatggca gcagtatttc tggcaggtat
     4561 cctgacatca tcctttttac tgaccggatg cggaaacagc acggcagata acagcagtgc
     4621 ttcttcctct tcatcagcat cttccgcaag tgcgtctggg gatcttttag agcagatcca
     4681 gagcaagggt gagatcgttg tggcaatgga aggaacctgg gcaccgtgga cgtatcacga
     4741 tgaggacgac aacctggtcg gctatgacgt agaagtggca cagcagatcg cagagaaact
     4801 cggcgtgaaa gcaacctttg tagagggtga gtgggacggc cttcttgcag gaatcgattc
     4861 cggccggtac gatatcatgg taaacggcgt agatatcaca gaggagcgcg ctgagaaata
     4921 cagcttttcc gacccatacg catacaaccg caccgctgtt atcgtaaact ctgctaacga
     4981 tgaaatccag tccatggagg atctcgacgg aaagagcacc gcgaacacca tttccagtac
     5041 gtatgcagaa gttgccgaga aatatggcgc agccgttacc ggtgttgacg atctggagca
     5101 gactttcgag cttctcttaa gtggccgcat cgatgcaact ctgaacgcag aggtaacgta
     5161 ttacgattat atgaaagcac acccggatgc agagatcaaa attgcatgtc tcgatccgga
     5221 atccacaaaa gtcggtattc cgatgaaaaa aggcgatgag accaagacgc tgcttgaggc
     5281 agtcaacaaa gcacttggcg agctcgcaga agacggaaca ctgacagagc tttcggaaaa
     5341 atattttgga acggatatct ccaaagaagc agcagaataa gatacaaaat aaagaaggtt
     5401 acagatcatg attgcaaatt atcataccca tacatggcgg tgccgccatg cggacggaac
     5461 ggagcgggag tatgtggagc gcgcgattga gggcggcctt aaaattctcg gtttttccga
     5521 ccattcgcct tatccgttcc cggacggcta cgattccggc atgcggatgc ggctcgatca
     5581 ggtggaaggc tatgtagaca ccgtgctggc attgaaaaaa gagtatgaca aggatattga
     5641 aatccatctc ggactcgaaa cagagtattt cccgagattc tgggatcagc tgattgattt
     5701 tttgtcggac tatccgtttg agtactttgt gcttggccag cacagcctgg gaaatgagat
     5761 cgaaaagatt ctctacagcg gccatggaac aacagacgga agctacctga aacagtatgt
     5821 ggaccagtgc ctggcaggca ttgataccgg aaaatacctg tatctggcac acccggatct
     5881 ctttttattc aacggcgacg aagcagtgtt cgaccgggaa taccggagac tctgccgtga
     5941 ggtgaaaaag agaaacattc cgctggagat caatttcctt ggtctgatgg aagaaagatg
     6001 gtatccgaac gagaagttct ggaagatcgc cggcgaagaa ggaaacaccg ccgttttcgg
     6061 ctgtgatgcc catacgccga aagccgtctg gaatccggag acggaacaaa aggcactgca
     6121 gctggcagca aaatacggtc tgaaagtaca ggaaaccgtt ccgatgccga agacatttta
     6181 acagaagcag aaaaagctgt tcttttctgg tgagagtttt gaaaactccg cggaaaagaa
     6241 cagctttttt gatatgagat tttttatgct ttttttgtgt gcatcagccg taccgcctct
     6301 tcccgctgtt tcagaatctt ctgaatcgaa gtccagtaaa cgatcattga gacaaccggc
     6361 ccgatcaggt cggaaatagg ttccacgtag aagatgtttt ccagtgcaaa gaaccgcggc
     6421 agaagaaaca gagcggtaaa gtagacgaat ttccggaaga aggagagtgc aaacgaatag
     6481 cgcacgcagc ccattgcagt aaagccgtct acaatctcgt actgaatgcc gagcggaatg
     6541 actgagagtg tcgaaatatg aatcgcgcgg accaccatcg agacgatctg cggatcggaa
     6601 gtaaaaagct gcgcaaacag cgttccggcg gcattggaga cgagaaacat caaggtcgta
     6661 aatccggcac agagtgcgat gatggttttc tgtgcctcaa gaatacgcct ggaatttcca
     6721 gctccataat tgtatgcgag gattgactgc gttccgccgc tgattccgcc gagcggcatc
     6781 gtaataatca gcataaagct ctgaacgatc gttgcacagg tgatgaagct gtctccctgt
     6841 ccggcgggtg cataccgctg caggacggcg ttcatcgcaa tgatcatcac attgtcgatg
     6901 gcaatgatta gaaacggagt aaaaccgact gtcagaatgc gtttcatgat cgcaaatgaa
     6961 tacccgccga aggtgatgcg gatcggaatc gtctttccga acagaatatg cagcacatac
     7021 agacagcttg cgatctggga gagcacggtt gcaattgccg cgccgcgcac gccgagatgc
     7081 agaacgaaga taaacacagg gtccagcagc agattcagga cggcgcctag cagaacagat
     7141 ttcattccct cactggcaaa gccctggcag atcacaaact ggttcaggcc ggtcgataaa
     7201 agcgcaaaca cagagccgga aacgtagatc ataaaatagg ctctggcgta aggatacgta
     7261 acggtgctgg ctccgaatgc catcagcatc gggcgggtaa aaaaaagcgc agcggctgtc
     7321 agaacgaggg acagggaaac aagcatcaga aacgcattag ccaggatgcg ggatgcctct
     7381 ttttcatttt tctctccgag ccggatgccg agcagcggtg cgccgccgac cccgatgagg
     7441 gaggagaacg cgccgatcat ggtgagaatg ggaccgcaga cgccgacacc cgcgagcgcc
     7501 agatcaccga cagaggcaat gtggccgatg tacatacggt ctacaatgct gtacagaaca
     7561 ctcacgaact gtgccagcat gctcggaaaa gcaatacggg gaaccagctt tccgatcggg
     7621 tcggttccga gatggtttac agttttcata gaaaaatacc ttgtcgtttt ttaggtttgg
     7681 gaacagacag caaaaaacat gcccgaaaca aaatccggaa gactttgttt cggacatgcc
     7741 gcttttggtc tgttctgata gatcagctga cagaaaaatt attcagcaag agtggtcaga
     7801 tctactttga aatcgtcaga tccctgtccg tctacggtgt agtaagcggc tctctgctct
     7861 gcgttcaggt agattttgat ctcgcctttt aatccgtttg cttttacttc ttttttgatg
     7921 actgcttcga ggtctttcat agatactttt gtctcgaaga tctcaagaac ggtatcagat
     7981 acttttgtca ttttacgtgg ggttttggtt gcttctgcca tggtttccgt ggtctccttt
     8041 tttactgttt ttttaacggt ttcttttttg gttgcttttt tcggctcttt tgcagcttct
     8101 gcttttcctt cggctgcttc tgtatgtaag gttttttcag tcgttggttt tacttctttt
     8161 gccggtgccg ctgtctttac aacggtttcc gctgtttttg cagcttctgc agcctgctgt
     8221 ttcgctttgg ccattttttt catggttctt ttattcattt tattcactcc tctatgcgat
     8281 atctctaata aattaccatg ttttttgcct tctgtcaaat ttaaattcga gaaaacagcg
     8341 gctttaggat tgtacacggc ggagcacaga aaagattttt tcagaatccc agatctgcgg
     8401 aacagggtag gtgggatttg cttcttttaa agcacggcgt gctatttctg tgtaatcatt
     8461 cggcgaaagg tgtggcagag tggatggaat gtcaaacgaa tcgcgcatag cctcgatagc
     8521 tgcaatcatc cgctcagcct tttcagaaac ggtatctccg ccgagagaaa gcgcatcggc
     8581 gagaacggca agtttaggcg caatgcagga accgtattcg cgcagaacag caggcagcag
     8641 aaccgcgttg gctcttccgt gcggaatgtg gtacagtgca ccgacggcgt gggcaatggc
     8701 atggacgtag ccgacaaagt tgttggtaaa agcgaaaccg gccaggtacg atgcatgaag
     8761 catttcctgc cgtgcagcaa gatgagtgcc gtctcgccag gcttcgggca gggcatggaa
     8821 attttcgcag actgcctcga tggatgcatt ctgcacgctg cgggaggcga agaggttcag
     8881 aaaagcttcc atggcatggg tcagggcatc cataccggta tctgcggtca tagaggacgg
     8941 gaggctgatc gtaagacgcg gatcgagaac tgccgtatcc gggatcagac agaggtcatt
     9001 gacggcgtat ttgtatggtt tcccgtcgat acagtctgtg atgacagctg ccgccgttgc
     9061 ctccgaaccg gttccggctg tcgtcggcac ggcaaacagc ggcggaagtt tccgacggac
     9121 tttcaggagg ccccgcatcg caggaaccgt ctttttcgga cagcaga
//
LOCUS       DAOMUF010000077         9145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1922_length_9145_cov_3.881298, whole genome shotgun sequence.
ACCESSION   DAOMUF010000077 DAOMUF010000000
VERSION     DAOMUF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_9145_cov_3.881298"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLN77_05875"
     CDS             <1..246
                     /locus_tag="PLN77_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRM43840.1"
                     /translation="ELKAEETTKSGIVLPGQAQEKPQQAEVIAVGPGKVEDGKEVKME
                     VAVGDQVIYSKYAGTNVKLEDEEYIIVKQDDILAVVK"
     gene            283..1908
                     /gene="groL"
                     /locus_tag="PLN77_05880"
     CDS             283..1908
                     /gene="groL"
                     /locus_tag="PLN77_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738769.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRM43841.1"
                     /translation="MAKEIKYGAEARNALQNGVNQLADTVRVTLGPKGRNVVLDKQYG
                     APLITNDGVTIAKEIELADGFENMGAQLIKEVAAKTNDVAGDGTTTATVLAQAMVNEG
                     MKNLAAGANPIVLRKGMKKATDKAVQILAEMSQPVAGKEQIAKVAAVSSGSEEVGQMV
                     ADAMEKVSKDGVITIEESKTMQNELELVEGMQFDRGYVSAYMCTDMEKMEAVLDNPYI
                     LITDKKLSNIQEVLPLLEQIVQSGARLLIIAEDVEGEALQTLVINKLRGTFNVVAVKA
                     PGYGDRRKAMLEDIAILTGGQVISEEVGLELKDATLEMLGRAKSVKVQKENTVIVDGA
                     GAKDAIAARIGQIRSQIEETTSEFDKEKLQERLAKMAGGVAVIRVGAATETEMKEEKL
                     RMEDALNATRAAVEEGIIAGGGSAYIHVTTQLAELIDSLDGDEKIGARIVQKALEAPL
                     FHIVTNAGLEGAVVINKVKESKVGVGFDAYNEEYVDMIQAGILDPVKVTRSALQNATS
                     VSATLLTTESVVSTIKEPAPAMPAGADGGMGMM"
     gene            2177..2848
                     /locus_tag="PLN77_05885"
     CDS             2177..2848
                     /locus_tag="PLN77_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43842.1"
                     /translation="MASRYESDMTRKEKMQLEKEKLSKMNFKEKLAYIWEYYKAVIFG
                     IIAVIFIIGTIVNIHENAKYYDLVSIAVVDYAGLQDVSPIEEDLKEALGTGDKYEKVS
                     IDTSYSFGENLENADYNTLMKFTAVIAAQSMDVLICSQAVYDNYSKDDYFLDLSTLFD
                     EATCEKYGIKAGDTCLDISKLKKYQDMGLTYYEPCYLTVVVNSKNTDNAAKLIEYLEE
                     DGVNE"
     gene            2841..3350
                     /locus_tag="PLN77_05890"
     CDS             2841..3350
                     /locus_tag="PLN77_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HRM43843.1"
                     /translation="MSDLYTEVLVKKQQTGKDKAIKGVLIFFTVLFAAAGIMMNPLIL
                     LLALVLGIVDYIFIPKLSVEFEYLYVNGELDIDRIYSQSRRKRAASYELSNMEILAPY
                     QSHQLDSYKNNQSIKRYNYSSGIEGQGHKPYAFVISKDNTMQMVIFEPDEVMLKDIRN
                     RAPRKVFMD"
     gene            3420..4877
                     /gene="guaB"
                     /locus_tag="PLN77_05895"
     CDS             3420..4877
                     /gene="guaB"
                     /locus_tag="PLN77_05895"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRM43844.1"
                     /translation="MGTIIGDGITFDDVLLVPQYSEVTPNMVDLTTHLTKKIKLNIPM
                     MSAGMDTVTEHRMAIAMARQGGIGIIHKNMSIEQQAEEVDKVKRSENGVITDPFYLSP
                     EHTLEDANNLMAKFRISGVPITENGKLVGIITNRDLKFETDFTKKIKESMTSENLITA
                     KVGVTLDEAKAILAKSRKEKLPIVDDDFNLKGLITIKDIEKTIKYPLSAKDEQGRLLC
                     GAAVGITANVLDRVDALVKAHVDVIVIDSAHGHSANIFKTLRLIKEHYPDLQVIAGNV
                     ATAEATRDLIAAGADAVKVGIGPGSICTTRVVAGIGVPQITAVMDCYEEAKKTGTPII
                     ADGGIKYSGDMTKAIAAGANVCMMGSIFAGCDESPGDFELFQGRKYKVYRGMGSIAAM
                     ENGSKDRYFQTGAKKLVPEGVEGRVAYKGLVEDTVFQLIGGLRSGMGYCGAKDVETLK
                     ETGKFVKISAASLKESHPHDIHITKEAPNYSVDDK"
     gene            4979..5689
                     /locus_tag="PLN77_05900"
     CDS             4979..5689
                     /locus_tag="PLN77_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HRM43845.1"
                     /translation="MAQKRRGKMTRKQRQRQLHRRMFLTGVLVVLICVGIYSGIRRVA
                     KTASDITAVDYSGSDYEDNDITDWTGAPPIDVELLTPNSWSRPQTRLKKVDGIVIHYT
                     ANPGSTAMQNRDYFENLPETQEAQASSHFVVGIEGEVVQCIPTSEWSYASNQRNFDTI
                     SIECCHPDDSGEFTDETYNSVVQLAGFLCKRFNLTSDDVIRHYDVTEKLCPLYYVEHE
                     DAWIQMKADIQAYADSLS"
     gene            complement(5787..6311)
                     /locus_tag="PLN77_05905"
     CDS             complement(5787..6311)
                     /locus_tag="PLN77_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM43846.1"
                     /translation="MNRIVIALLKPFSFIPAILMMSLIFSFSSQNGTESSQLSEKVAR
                     GIFITIDHVMDRGWDDEKIQELADEYQYPVRKLAHMTEYCLLAICVSLPLYVYGLRGF
                     ALTFLAGILCVAFAASDEFHQTTVANRGPSVKDVGIDSIGVLFGITFVRLFCWIALGG
                     NTKKRKRRRRRYYY"
     gene            complement(6355..7179)
                     /locus_tag="PLN77_05910"
     CDS             complement(6355..7179)
                     /locus_tag="PLN77_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRM43847.1"
                     /translation="MISNLLKMLSYPFMQRALVVGILVSLCAALLGVSLVLKRYSMIG
                     DGLSHVGFGSLAIATALGFAPLAFTIPVVVLAAFLLLRMNESSKIKGDAAIGLISSSA
                     LAIGVITVSWSSGMNTDVNNYMFGSILAMSDEDVVLSVVLSIVVLILFVLYYNRIFAV
                     TFDETFAKATGVRAEVYNMLLAFLTAITTVLGMRMMGALLISSLLIFPALTSMRLCHT
                     FQSVVICSAVLSVVCFLLGMSLSFFLSSPAGATVVIVNLLAFLIFSAIARIREHTA"
     gene            complement(7176..7883)
                     /locus_tag="PLN77_05915"
     CDS             complement(7176..7883)
                     /locus_tag="PLN77_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRM43848.1"
                     /translation="MALLTCSDVTFSYEGIPVLTDVNFALEAGSYLCIVGENGSGKST
                     LVKGLLHLKNPSTGSITTGDGLHPSEIGYLPQQTAAQKDFPASVWEVVLSGRQNHPHF
                     PPFYTKADKEDALRNMELLDLLPLKKRCYRDLSGGQQQRVLLARALCATKKLLLLDEP
                     VTGLDPVMTNEMYQLIRRINREQNVTIIMVSHDIRNILPDATHILQLDQKQVFFGPMD
                     EYLKTEAGKQFLGGADL"
     gene            complement(7883..8899)
                     /locus_tag="PLN77_05920"
     CDS             complement(7883..8899)
                     /locus_tag="PLN77_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRM43849.1"
                     /translation="MTFFSRKHLRLLLLPALLLLTGCAGSAKETADSTENEKLSAVAT
                     IFPQYDFLRAIGGDHLDLHMLLSPGAESHSFEPTPGDMITVSECDLFVYVGGDSDAWV
                     ETILDSVDTSNKEVVTLMDCVDTVAEETVEGMETHGHAHDHEDEDTDHDEDSHDHEDE
                     SHGEQDEHVWTSPKNAMRIVQKLCDALCRLDPENADDYQANTASYLASLTSLDEEFSD
                     VTANASRHTIVFGDRFPFRYFADAYGLDYYAAFPGCSSESEASAKTLSFLIDKINEEQ
                     IPVVFHTELSNEKMTDCICEATGAKKLLLHSCHNVSKDDFENGATYLSLMEQNVAALK
                     EALN"
BASE COUNT         2594 a         2087 c         2513 g         1951 t
ORIGIN      
        1 gagttaaaag cagaggaaac cacaaaatcc ggtatcgtac ttccgggaca ggcacaggag
       61 aaaccgcagc aggctgaggt tatcgcagta ggacctggaa aagtagaaga cggcaaagaa
      121 gtaaaaatgg aagttgcagt cggagatcag gtaatctatt ccaaatatgc aggaaccaac
      181 gtcaaactgg aagacgaaga gtacatcatc gtaaaacagg atgacattct ggcagttgta
      241 aaataaagaa taatcaagta aaaatcagga ggcatgacaa tcatggcaaa agagattaaa
      301 tatggagcag aggcaagaaa cgctcttcag aatggtgtaa accagcttgc agataccgta
      361 cgtgtaaccc ttggaccgaa aggaagaaac gttgttctgg acaaacagta cggcgctccg
      421 ctcatcacaa acgatggtgt tacgattgca aaagagatcg agctggcaga cggctttgaa
      481 aacatgggcg cacagctcat caaagaagtt gctgcaaaga ccaacgacgt agcaggtgat
      541 ggtaccacaa ccgcaaccgt tctggcacag gctatggtaa acgaaggaat gaaaaacctg
      601 gcagcaggtg cgaacccgat cgttcttcgt aagggaatga agaaagcaac cgataaagct
      661 gttcagatcc ttgcagagat gagccagccg gtagcaggca aagagcagat tgcaaaagtt
      721 gcagctgttt cttccggaag cgaagaagta ggccagatgg tagctgacgc tatggaaaaa
      781 gtttccaaag acggcgttat caccatcgag gagtccaaga ccatgcagaa cgagctggag
      841 ctggtagaag gtatgcagtt cgaccgtgga tatgtctctg cttacatgtg taccgatatg
      901 gagaaaatgg aagccgttct cgacaatcca tatatcctga ttaccgataa gaaactgtcc
      961 aacattcagg aagttcttcc gctgctggag cagatcgtac agagcggcgc aagactgctg
     1021 atcatcgctg aggacgtaga gggcgaggct ctccagacac tcgtgatcaa caaactgcgc
     1081 ggaaccttca acgtagtagc tgtcaaagca ccgggatacg gcgacagaag aaaagcaatg
     1141 ctcgaggata tcgcaatcct gaccggcggc caggtgatct ccgaggaagt tggtctggaa
     1201 ttaaaagacg ctactctgga aatgctcgga cgtgcaaaat ctgttaaagt tcagaaagag
     1261 aacaccgtta tcgttgacgg tgccggcgca aaagatgcca tcgcagcaag aatcggccag
     1321 atcagatccc agatcgagga gaccacttct gagttcgata aagagaaact gcaggagaga
     1381 ctggctaaaa tggcaggcgg cgtagctgtt atccgtgtag gtgcagcaac cgagaccgag
     1441 atgaaagaag aaaaactccg tatggaggat gctttaaatg caacgagagc agccgtagaa
     1501 gagggtatca tcgcaggcgg cggttctgca tacattcatg taacaacaca gcttgcagag
     1561 ctgattgaca gcctggacgg cgacgagaag atcggtgcaa gaatcgtaca gaaagcactc
     1621 gaggctcctc tgttccacat cgtaacaaac gcaggtctgg aaggcgctgt tgtgatcaac
     1681 aaagtaaaag agtccaaagt aggtgttgga tttgatgcat acaacgaaga gtatgtagac
     1741 atgatccagg ctggtatcct tgatccggtt aaggttacca gaagcgcact gcagaacgct
     1801 accagcgttt ccgcaaccct gctgaccacc gagtccgtag tttccaccat caaagagccg
     1861 gctccggcta tgccggcagg cgcagacggc ggaatgggaa tgatgtaatt tccgggataa
     1921 aaatgtctgg taaaacaaaa taagaaaacc ggctggcaaa gaccgtagcg gaacatgggt
     1981 tccgaagtcc tgtctttgcc ggccggtttt ttagtggaaa gtttttcttc catttcgcat
     2041 ggctctgaat cgttacaaaa aatttcagaa agttttcatg ctttcgccgg gcggcgtata
     2101 tggacgcttc cggcgtttta tgctatactg atttgtagga tgttggggga aatacaacaa
     2161 aggagaagaa acacacatgg caagtcgtta tgaatccgac atgacaagga aggagaagat
     2221 gcagctcgaa aaagagaagc tgtcgaaaat gaattttaaa gagaaactgg cctatatctg
     2281 ggaatactac aaggctgtca ttttcggcat catcgctgtt atcttcatca tcggcacgat
     2341 tgtgaatatc catgaaaatg cgaagtatta tgacctcgtg agcattgccg tagtggatta
     2401 cgcaggactg caggatgtaa gtccgatcga ggaggaccta aaagaagcgc tcggaaccgg
     2461 agataagtac gaaaaggtaa gcatcgatac gagctacagc ttcggtgaaa acctggaaaa
     2521 cgcggattac aacacgctga tgaaattcac agccgtgatt gccgcacaga gcatggatgt
     2581 tctgatctgt tcacaggccg tgtacgacaa ctacagcaaa gacgattatt ttctggatct
     2641 gtccacactg tttgacgaag cgacgtgtga gaagtacggg ataaaggcgg gagatacctg
     2701 cctcgatatt tccaagctga agaaatacca ggatatgggc cttacgtact atgagccgtg
     2761 ttacctgacc gttgtggtca actccaagaa cacggacaat gcagcaaaac tgatcgaata
     2821 tcttgaggag gatggagtaa atgagtgatc tgtacacaga agtgctggtg aaaaaacagc
     2881 agacaggaaa agacaaggcg atcaaaggag ttctgatctt ctttaccgtt ctttttgcag
     2941 ccgcaggaat tatgatgaat ccgctgattc tgcttcttgc gctggtactt ggaatcgtag
     3001 actatatttt tatcccgaag ctttcggtag agtttgagta tctgtacgtc aacggcgagc
     3061 tcgacatcga ccgtatctat tcgcagtcaa gaagaaaaag agccgcaagc tatgagcttt
     3121 ccaacatgga aatcctggct ccgtaccagt cccatcagct cgattcctac aaaaacaatc
     3181 agagcatcaa acgttacaac tattcctccg gaatcgaagg acagggccac aaaccgtatg
     3241 catttgtgat ctccaaggac aacacaatgc agatggtgat cttcgaaccg gatgaagtga
     3301 tgttaaaaga tatccgcaac cgcgcaccaa gaaaagtatt tatggattga cagctttttt
     3361 gttttgtgtt aaattaggtg ataaatcaaa aagacttaag aagaaaggaa gataagaaaa
     3421 tgggtacaat tattggtgat ggtattacct tcgatgatgt gcttctggtt ccgcagtatt
     3481 ctgaagtaac cccaaacatg gtagatctga ctacgcatct gaccaagaag atcaagctga
     3541 acatcccgat gatgagcgcc ggcatggata cggttacaga acaccgtatg gcaattgcca
     3601 tggcacgtca gggaggaatc ggtattattc acaagaatat gtcgattgag cagcaggcag
     3661 aagaagtaga caaagtaaaa cgttctgaga acggtgttat caccgatccg ttctatctgt
     3721 ctccggagca caccctggaa gatgccaata atctgatggc aaaattccgt atttccggtg
     3781 tcccgattac cgagaacgga aaactggttg gtattattac aaaccgtgat ctgaagtttg
     3841 aaactgattt caccaagaaa atcaaagagt ccatgaccag cgaaaacctg atcactgcaa
     3901 aagtaggcgt tacgctggac gaggcaaaag caatccttgc aaaatccaga aaagaaaaac
     3961 ttccgattgt cgatgacgat ttcaacctca aaggtctgat caccatcaaa gatatcgaga
     4021 aaaccatcaa gtacccattg tccgccaagg atgagcaggg acgtctgctc tgcggtgccg
     4081 cagtcggcat cacggcaaac gtgctcgatc gtgtcgatgc gctggtaaaa gcacacgtag
     4141 acgtgatcgt catcgactcc gctcacggac attccgcaaa tatcttcaaa acacttcgtc
     4201 tcatcaaaga gcattatccg gatctgcagg taatcgccgg aaacgtggca accgcagagg
     4261 caacaagaga cctgatcgca gccggagcag acgctgttaa agtcggtatc ggacctggtt
     4321 ctatctgtac gacccgtgtc gttgcaggta tcggtgtacc gcagatcaca gccgttatgg
     4381 actgctatga ggaagcaaag aagaccggaa ctccgatcat cgccgacggc ggtatcaagt
     4441 actccggaga catgacaaag gctatcgcag ccggtgccaa tgtctgcatg atgggaagca
     4501 tcttcgcagg atgcgatgag agcccgggag atttcgaact gttccaggga agaaaataca
     4561 aagtataccg tggaatggga tccatcgcag ccatggagaa cggaagcaaa gaccgttact
     4621 tccagaccgg cgccaagaaa ctggttccgg aaggtgtgga aggccgtgta gcatacaaag
     4681 gcctcgtaga ggacactgta ttccagctga tcggcggact tcgttccggt atgggctact
     4741 gcggagcgaa agatgttgag accttaaagg agaccggaaa atttgtgaag atttccgcag
     4801 catccttaaa agagtcccat ccgcatgata tccacatcac caaagaggct ccgaactaca
     4861 gcgtggacga taaataagaa taaaaagtcg caaaaggcag aagggggaaa gggcagaaac
     4921 ttttcagctt ctgccttttc aaatgagggg gcgcttattt gcagcgggga gagaacatat
     4981 ggcacagaag agacggggaa aaatgacaag aaaacagagg cagcgccagc ttcaccgcag
     5041 aatgtttctg acgggagttc tggtggtgct gatctgcgtc ggcatttaca gcggcatccg
     5101 ccgtgtggca aaaacggcaa gtgatatcac ggccgttgac tattccggtt ctgattatga
     5161 ggacaacgat atcaccgact ggaccggcgc tccgccgatt gatgtggagc ttctgacgcc
     5221 gaacagctgg tccagaccgc agacgaggct gaaaaaggtg gacggtatcg ttattcacta
     5281 caccgcaaac ccgggaagca cggcgatgca gaaccgtgat tactttgaaa acctgcctga
     5341 gacgcaggag gcgcaggcca gcagccattt cgtcgtcggc atcgagggag aagtcgttca
     5401 gtgtatcccg acgagtgaat ggtcgtatgc atcgaaccag cgtaatttcg atacaatctc
     5461 gattgaatgc tgccacccgg atgacagcgg tgaatttacc gatgagacat acaattctgt
     5521 ggtccagctc gccggtttct tatgcaagcg gttcaatctg acttcggacg atgtgatccg
     5581 tcactatgat gtaaccgaaa agctctgccc tctgtactac gtggagcatg aggatgcctg
     5641 gattcagatg aaggcagata ttcaggcgta tgcggattca ctttcctaag agctgttttt
     5701 aagaaaaatg aaaaaatccc cattgggaaa atcagtttca ggagaaatga tttttccaac
     5761 ggggattttt ctgttatcca atatttttag taatagtagc gccgtctgcg ccgctttctt
     5821 tttttcgtat ttccgccgag tgcaatccag cagaacagcc gtacaaacgt gatgccgaag
     5881 agaacgccga tgctgtcgat gccgacgtcc tttaccgacg ggccacggtt ggcgaccgtc
     5941 gtctggtgga actcatcgct cgcggcaaag gcgacacaga ggatgccggc aagaaacgtg
     6001 agcgcgaagc cgcgcaggcc gtagacgtag agcggaagcg aaacacagat tgcaagcaga
     6061 cagtactctg tcatatgagc cagcttccgc accgggtact gatactcgtc agccagttcc
     6121 tggatctttt catcatccca tccgcggtcc atgacgtggt cgatcgtgat gaaaatgccg
     6181 cgcgccactt tttcactgag ctgtgaggat tccgttccgt tctgggaaga gaaggagaaa
     6241 atcagcgaca tcatcagaat cgccgggatg aaggaaaacg gttttaacag tgcaattaca
     6301 attcgattca taaaatacct ccggtaaaac aaagcaggtc agatcggaaa ctttttatgc
     6361 ggtatgctcg cggattctgg caatcgcaga aaagatcagg aatgccagca ggttcacgat
     6421 tacgaccgtc gctccggcag gggaggacag gaaaaacgag agggacatgc caagaaggaa
     6481 gcacaccacc gagagaaccg cggagcagat caccacgctc tggaaggtat ggcagagacg
     6541 catcgaggtc agtgccggga aaatcagcag gctggagatc agaagcgcgc ccatcatgcg
     6601 catgccgagg acggtggtga tggcggttaa gaatgccagc agcatgttgt aaacctctgc
     6661 gcggacaccg gttgctttgg caaatgtctc gtcgaaagtg acggcaaaaa tacggttgta
     6721 atacaggacg aacaggatca gcacgacgat ggagagcacc acggagagaa caacgtcctc
     6781 atcactcatc gcaaggatgc ttccgaacat gtagttgttg acatcggtgt tcatgccgct
     6841 cgaccacgaa accgtgatga caccgatcgc aagcgcagag cttgaaatca ggccgatggc
     6901 ggcgtcgcct ttgatcttgc tggattcgtt catgcggagc agcagaaagg cggcaagcac
     6961 gacgacaggg attgtgaagg caagcggagc aaaaccgagt gccgttgcaa ttgcaagaga
     7021 gccgaatccg acatgggaga ggccgtcgcc gatcatggag tagcgcttta acacgaggct
     7081 gacgccaagc agtgcggcac acaaagagac gagaatgccg accacaaggg cacgctgcat
     7141 aaacggataa gaaagcattt tcagcagatt tgaaatcata agtcagcacc tcctaaaaac
     7201 tgttttcccg cctcggtttt cagatactcg tccatcggac cgaagaagac ctgcttctgg
     7261 tcgagctgga gaatgtgcgt tgcgtccggc agaatattgc ggatatcgtg ggaaaccatg
     7321 atgatcgtaa cgttctgctc gcggttgatg cggcggatga gctggtacat ttcgtttgtc
     7381 atgaccgggt caaggccagt gaccggctcg tcgaggagca gcagtttctt tgtggcgcaa
     7441 agcgcacgag cgagaagcac acgctgctgc tggccgccgg agagatcacg gtagcatcgt
     7501 ttctttaatg gaaggaggtc gagaagctcc atattgcgga gcgcgtcctc tttgtctgcc
     7561 tttgtgtaaa acggcgggaa atgcgggtgg ttctgccggc cggacagcac gacttcccag
     7621 acgctcgccg ggaaatcctt ttgtgcggcg gtctgctgcg gcagatagcc gatctcgctt
     7681 ggatgaagcc cgtcgccggt tgtgatcgag ccggtggacg gattttttaa gtgaagcagt
     7741 cccttgacca gagtactttt tccggaaccg ttctcgccca caatacagag gtaggagccg
     7801 gcttccaggg caaagttgac atcggtcagg accgggatgc cctcgtagga gaaggtgaca
     7861 tcactgcagg taagtagagc cattagttca gagcctcctt tagtgcggcg acattctgtt
     7921 ccatcaggga aagatacgtg gcgccgtttt cgaaatcatc tttggatacg ttgtgacagg
     7981 agtgaagcag cagctttttc gcgccggtcg cctcacagat gcagtccgtc attttttcgt
     8041 tggacagctc ggtgtggaag acgacaggaa tctgctcttc gttgatcttg tcaattaaaa
     8101 aagaaagtgt tttggcactc gcctcggatt ccgaggagca gccggggaat gccgcgtaat
     8161 agtcgaggcc gtaggcgtcc gcgaaatagc ggaacggaaa acggtcgccg aaaacgatgg
     8221 tgtggcggct ggcattcgct gtgacatcgg aaaattcctc atcgagggag gtaagcgaag
     8281 cgaggtatga agcagtgttt gcctgataat cgtccgcgtt ttccgggtcg aggcggcaga
     8341 gcgcatcgca gagcttctgg acaatccgca ttgcattttt cggtgaagtc cagacgtgct
     8401 cgtcctgttc gccgtgggat tcgtcttcgt gatcgtggga atcctcgtcg tggtccgtat
     8461 cctcgtcctc atgatcgtgt gcgtggccgt gggtctccat tccctcgacc gtttcctcgg
     8521 caacggtgtc gacgcagtcc atcagcgtga cgacttcttt gttcgaggta tcgacggaat
     8581 cgagaatcgt ttccacccag gcatcggaat cgccgccgac gtagacgaag agatcgcatt
     8641 cggaaactgt gatcatatcg cccggtgtcg gctcaaatga gtggctttct gcgcccgggg
     8701 aaagcagcat gtggagatcg aggtgatcgc cgccgatggc acgcagaaaa tcgtactgcg
     8761 gaaaaatggt cgccacggcg gagagctttt cgttctctgt ggaatccgcc gtctcctttg
     8821 cggaacctgc gcagcccgtc agaagaagga gcgcaggaag gaggagaagg cgtaaatgtt
     8881 ttctggaaaa aaacgtcatg atactccttt ctgaaagaaa acacggaggc attgtgaaaa
     8941 agctccgtat cagaccgttc agtaacaaat gccgcaggag tgggaagcgg cagacggaga
     9001 gtgtacccct ccgaaaaaag atcagttacc atcatatagc aggggtgatt catttgtcaa
     9061 ggaaagacac aaaaagctgc gtccgccaga agcggggaag cagctttttg cagatatttg
     9121 atacgttttg tgaaaccgga ttacg
//
LOCUS       DAOMUF010000078         9133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1928_length_9133_cov_3.201366, whole genome shotgun sequence.
ACCESSION   DAOMUF010000078 DAOMUF010000000
VERSION     DAOMUF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1928_length_9133_cov_3.201366"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..895)
                     /gene="recO"
                     /locus_tag="PLN77_05925"
     CDS             complement(152..895)
                     /gene="recO"
                     /locus_tag="PLN77_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRM43850.1"
                     /translation="MREPINVSGMVLSASPVGEYDKRVVILTRELGKITAFARGARRM
                     KSPLMAVSNPFVFGEFQVYEGRDSYTLSGANIKEYFLDLAQMQPGVYYGFYFLELAEY
                     FGQEGNDETQMLNLLYVTMNAIRRGKQDLRLIRCIFELRTITISGEAPQMFSCMECGT
                     KENLTVFSLRDSAIYCPNCGKTIRDPWPLQPGTVLACRYIIAAPLAKLYGFTVNEEIL
                     DELERLLRAYLHRYVEKKMKTLQILDVMK"
     gene            complement(921..1838)
                     /gene="era"
                     /locus_tag="PLN77_05930"
     CDS             complement(921..1838)
                     /gene="era"
                     /locus_tag="PLN77_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRM43851.1"
                     /translation="MENNANYKSGFVALIGRPNVGKSTLMNHLIGQKIAITSKKPQTT
                     RNRIQTVYTCDEGQIVFLDTPGIHKAKNKLGEYMVDVAEKTLKEVDVILWLVEPSNFI
                     GAGERHIAEILEKVNAPVILIINKTDTVEKDKILEFIDTYRKLFQFAEIIPCSALRHQ
                     NTDDIIPSIFKYLPYGPQFYDEDTITDQPMRQIVAEIIREKALYALNEEIPHGIAVVI
                     DQMKDRPNGKIVDIDATIICERDSHKGIIIGKQGAMLKKIGSNARYELEQMLEQKVNL
                     KLWVKVKKDWRDSDFLVKNFGYDKKKLDL"
     gene            complement(1858..4773)
                     /locus_tag="PLN77_05935"
     CDS             complement(1858..4773)
                     /locus_tag="PLN77_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRM43852.1"
                     /translation="MNAEHCKAYTLLQEQQIDDIHAHGYLLRHNKSGARVLLLENEDD
                     NKVFNIAFRTPPADSTGVAHILEHSVLCGSREFPLKDPFVELVKGSLNTFLNAMTYPD
                     KTMYPVASTNDADFQNLMHVYMDAVFYPNIYKHDEIFRQEGWSYHLESEDGPLTYNGV
                     VYNEMKGAFSSADDVLERETFNTLFPDTPYGVESGGDPSCIPNLTHENFLNFHQTYYH
                     PSNSYIYLYGNMDMEEKLAWMDEKYLSHFNAKEVKSEIPYQKPFAETLDIVHEYPVLD
                     GDPLENNAYLSYNMVIGSGLDVKLNVAFSVLEYALLDAPGAPVKQALLDAHIGKDVYG
                     SYEDGILQPFFSIVAKNADENEKEKFLSIIRGTLEDIVKNGMDQKAIEAGINYFEFRF
                     READFSSFPKGLMYGIDVFDSWLYDENKPFAYLQQLAIYDELKKLAKEGYFENLIQTY
                     LLDNTHASIVTLIPKTGLAAENDAKTAEKLQKYKESLSKEEIEKIIADTKELAAYQEE
                     EESEEALETIPLLKRSDIKRESIKLYNDEHEVDGTTVLHHNVFTNGIGYLSLLFDTKN
                     VPNDLIPYMGVLKSVLGYVDTEHYTYGELFNEINAQTGGINCGLQVFRIPENDDDCRR
                     MFGIRAKFLYDKLDFVMKMIEEILNTSRLDDEKRLHEIISSMKSGLQNRLSSAGNATA
                     VMRAASYYSPMSNFQDRIAGIGFYQLLKDLDENFDEKKAELIKNLQTLMKYIFRKENL
                     TVSYTADETGYAPLEEKIAAFKENLYTDEVEPGSIVYDFEQKNEAFQTSGQVQYVALC
                     GNFEREGYDYTGALRILRVMLSYDYLWINIRVKGGAYGCGGAFGRGGNACISSYRDPN
                     VGRTLEVYRGIGDYVRNFEADERQMTKYIIGTISELDVPMNPSAKGQTSESAWFNGIT
                     EADFQQERDQILDATLDDIHALADLVDAALKQNNICVVGSEAAIQKEKELFKNVENL"
     gene            complement(4877..5842)
                     /locus_tag="PLN77_05940"
     CDS             complement(4877..5842)
                     /locus_tag="PLN77_05940"
                     /inference="COORDINATES: protein motif:HMM:NF018837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HRM43853.1"
                     /translation="MTVDNSFTMKKFQSMEIIYVTFSQITKLPYVECDPETFDDQVYM
                     FTEEEAAKEFAKSYVEKNTPLLTVKVLRKQMPNFYMGLYAEGVNMVIFREGDQTRRIE
                     LEQIFPKPDMEKMNKQHLPVLNPGVQLTVVYFLQELRKPNQRRDDAERMQHLRELEEE
                     MLVNLMRSKFILAIDISQVQGEFDPANPGPDVRIPYIKNQNEDIFQPLFSDIGEFQKF
                     RPDPQAKLRLAAIPFQHLLPYLMKQAKGFVINPSGFNLLLTREQLQRIMGAQAQSVRP
                     NLEAMMAQAAAAQAQKNAQNNAANDEVKEHVDETENTAAETDNEE"
     gene            complement(6069..7376)
                     /locus_tag="PLN77_05945"
     CDS             complement(6069..7376)
                     /locus_tag="PLN77_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HRM43854.1"
                     /translation="MNYTIYDYLGLFFFYAFLGWLLETSVAAIRKKHMVNRGFLNGPL
                     CAIYGITAVFMTRYLYELQSSPVFLFLGCMIIATAVEWLAGHALERIGHGKWWDYSNK
                     KWNMDGYICLQYSVLWGILGLLAMKIGNRLCFTVLHLIPAGFLHITSWILFGILVVDA
                     VGSYAVLHHITKKMPRISAMNDQIDAFTRRLSVRVYRYLERRVERAYPAVQPVPEKKE
                     KTETGVFAEGCSFYKIVLLFIIGAFLGDITETIFCRITAGVWMSRSSLVWGPFSIVWG
                     LAIALATWFLYNYRDRSDGFLFAFGTFLGGAYEYICSVFTEIVFGKVFWDYSDIPFNL
                     GGRINLLYCFFWGIAAVVWLKKFYPVISRWIEKIPMKFGEIITWILIVFMVANMLVST
                     LALARYDERAHQKPAANAIEQTIDAHFPDARMEKIYPNAKAIN"
     gene            complement(7407..8384)
                     /locus_tag="PLN77_05950"
     CDS             complement(7407..8384)
                     /locus_tag="PLN77_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HRM43855.1"
                     /translation="MKGKKRVIIQNNRVHYEFVIKRNITIIQGDSASGKTTLVNMIRQ
                     AENLGESSGINVSCEVPCRVLEGVNWKIILENSRESIFFIDEENYFIKTEEFASAIRG
                     SENYFVLITRENLYNLPYSVEEIYGLHVSGKYRDTRKIYQKMYQIYPKTARLPVRVQK
                     IITEDSNSGYQFFENVCAERNITCESAGGKTKIPEMIGKAGTEETCIIADGAAIGAEM
                     NLLSNKIEEKQNVHLYLPESFEWLILNSDLLQDKEVRKILENPEDYIDSTEFFSWEQY
                     FTKLLIQKTEGTYLQYKKAKLNPSYLQDKNKNKILESIQGIQWKIKSLK"
     gene            complement(8393..8833)
                     /locus_tag="PLN77_05955"
     CDS             complement(8393..8833)
                     /locus_tag="PLN77_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4869 domain-containing protein"
                     /protein_id="HRM43856.1"
                     /translation="MLKIYLGEMKGAIYHPPTYFDNCYEDEWLTTPLAKEMIRDVDRS
                     EVISAHLIDSPVLGPIPPKDLSGGIKTLLLMAYDTDGKIFNASVCGDNCAKWILKLGE
                     QKDLTINLRHVMRFGKDPFEILILNTGTIVRNMSEFVDVAEAYV"
BASE COUNT         2021 a         2115 c         2231 g         2766 t
ORIGIN      
        1 tactatgcat ttgtaatatc ttctgtgacg aatacagaac catttttttc tttattccaa
       61 atattctatc ccatttctac ttatttgtaa aggtgtggac acacttttgg acataattat
      121 ttcacatttt tgcaaattat tacattttat attatttcat cacatccaga atctgtaatg
      181 ttttcatctt cttttcgaca tagcgatgga gatatgcacg cagcaatcgt tccagttcat
      241 ccaggatctc ttcatttaca gtaaaaccat acagcttcgc cagcggtgcc gcaatgatat
      301 agcggcatgc cagcacggtg cccggctgca gcggccatgg atctctgatg gtttttccac
      361 agtttgggca gtagatggcg ctgtcgcgca gggagaaaac ggtcagattc tctttcgtgc
      421 cgcactccat gcaggagaac atctgcggcg cttctccgct gattgtgatc gtccgcagtt
      481 cgaagatgca ccggatcagg cggaggtcct gttttccgcg gcggatggcg ttcatcgtca
      541 cgtacagaag gttcagcatc tgggtttcat cgtttccttc ctggccgaaa tattcggcca
      601 gttccagaaa ataaaagccg taataaacgc cgggctgcat ctgcgccagg tcgaggaagt
      661 attctttaat gtttgcgccg gagagggtgt agctgtcgcg gccttcgtag acctgaaact
      721 cgccgaagac gaacggattg gagacggcca tcagcgggct tttcatgcgg cgggcgccgc
      781 gcgcgaaggc ggtgattttg ccgagctccc gggtcaggat cacgacgcgc ttgtcatact
      841 ctccgaccgg ggaggcggat aataccattc cgcttacatt gattggttct cgcatgtttc
      901 ttccttatta taatgcaata ttataaatcg agtttctttt tatcatatcc gaagttcttc
      961 acaaggaaat cgctgtcgcg ccaatctttt ttaactttta cccagagctt tagattgact
     1021 ttctgctcca gcatctgctc cagctcatac cgcgcgttgc ttccgatctt cttgagcatc
     1081 gcgccctgtt ttccgatgat gatgcccttg tgggaatcgc gctcgcagat gatggtcgca
     1141 tcaatatcaa cgatttttcc gttcgggcgg tctttcatct ggtcgatcac aacggcgatg
     1201 ccgtgcggga tctcttcgtt taaggcgtac agtgctttct cgcggatgat ctcagccacg
     1261 atctggcgca tcggctgatc ggtgatggtg tcctcatcgt agaactgcgg gccgtacggc
     1321 agatatttga agatgctcgg aatgatgtca tcggtgttct gatggcggag tgccgagcac
     1381 ggaatgatct cggcaaactg gaacagcttg cggtacgtat cgatgaattc caggattttg
     1441 tctttctcca cggtatcggt cttgttgata atgaggatga ccggcgcatt gactttctcc
     1501 aggatctctg cgatatggcg ctctcctgct ccgatgaagt tgctcggctc taccagccag
     1561 agaatcacat cgacttcttt taacgttttc tcggctacat cgaccatgta ctcgccgagc
     1621 ttatttttcg ccttgtggat acccggtgtg tcgaggaaca cgatctgtcc ctcgtcacac
     1681 gtataaacgg tctggatgcg gttgcgggtg gtctgcggtt tcttggatgt aatcgcaatc
     1741 ttctgtccga tcaggtggtt catcagcgtg gattttccca cgttcggacg gccgatcagg
     1801 gctacaaagc cggatttata atttgcattg ttttccattg ctgtctcttc tcctcattta
     1861 taagttctct acgttcttaa acagttcttt ctctttctga atggctgctt cgcttccgac
     1921 aacgcagatg ttgttctgtt ttaaggctgc atctacaagg tctgccagtg catgaatgtc
     1981 atccagggtt gcatcgagga tctgatctct ctcctgctgg aagtctgctt cggtgatgcc
     2041 gttgaaccat gcgctctcgg aggtctggcc ttttgcggac gggttcatcg ggacgtcgag
     2101 ttcgctgatc gttccgatga tgtatttggt catctggcgc tcgtctgctt cgaaattgcg
     2161 cacgtaatcg ccgataccgc ggtatacttc aagggttctt ccgacgttcg ggtcacggta
     2221 ggaagaaatg cacgcgtttc cgcctcttcc gaaagctccg ccgcagccgt aagcgccgcc
     2281 tttgacacgg atgttgatcc acagatagtc atagctcagc atgacacgca ggatgcgcag
     2341 ggctcctgtg tagtcgtagc cttcgcgctc gaagtttcca cagagtgcca cgtactgtac
     2401 ctggccggag gtctggaagg cttcgttttt ctgttcaaag tcatacacaa tgctgcccgg
     2461 ctccacttcg tcggtgtaca gattctcttt gaatgctgcg attttttctt ccagcggtgc
     2521 gtagcctgtc tcgtctgcgg tataggagac ggtcagattc tctttgcgga agatgtattt
     2581 catcagggtc tgcagatttt taatcagttc tgcttttttc tcatcgaagt tctcgtccag
     2641 atcttttaag agctggtaga agccgatgcc tgcgatgcgg tcctggaaat tgctcatcgg
     2701 ggagtaatag gaagctgcac gcatcacagc cgttgcattt ccggcggaag acaggcggtt
     2761 ctgcaggccg gatttcatgc tggagatgat ctcgtgaaga cgtttttcat cgtccaggcg
     2821 ggaggtgttc aggatttcct cgatcatttt catgacgaaa tccagtttgt cgtacaggaa
     2881 tttcgcgcgg atgccgaaca ttctgcggca gtcgtcatca ttttccggaa tgcggaatac
     2941 ctgcagtccg cagttgatgc cgccggtctg tgcattgatc tcgttgaaca gttcgccgta
     3001 ggtgtagtgt tcggtgtcca catagccgag aacggatttt aatacgccca tgtacgggat
     3061 cagatcgttc ggcacatttt tcgtatcaaa cagcaagctt aagtaaccga tgccgttcgt
     3121 gaatacgttg tgatgaagaa ccgtcgttcc atcgacttca tgctcgtcat tgtaaagttt
     3181 gatagactca cgtttgatat cgcttcgttt caggagcgga atcgtctcga gtgcttcctc
     3241 gctctcctct tcttcctgat atgctgccag ctctttggtg tcagcaatga ttttctcgat
     3301 ctcttctttt gacaggctct ctttgtattt ctgcagtttt tctgcggtct ttgcatcatt
     3361 ttctgcggca agaccggtct ttggaatgag ggtcacgatg gaggcatggg tgttgtccag
     3421 cagatacgtc tggatcaagt tttcaaaata gccttctttt gccagttttt tcagctcatc
     3481 gtagatcgca agctgctgca ggtaggcgaa cggtttgttc tcgtcgtaca gccagctgtc
     3541 gaagacgtca atgccgtaca tcaggccctt cgggaagctg gagaagtccg cctcacggaa
     3601 acggaattcg aaatagttga tgcctgcctc gatggctttc tgatccatgc cgtttttcac
     3661 gatatcctcg agggtgccgc ggatgatgga caggaatttt tctttctcat tttcatcggc
     3721 gttcttggct acgatggaga agaatggctg caggatgccg tcctcgtagg agccgtaaac
     3781 atcttttccg atgtgtgcgt caaggagtgc ctgtttgacc ggggctcccg gtgcgtcaag
     3841 gagtgcatac tccagaacag aaaatgccac gtttaatttt acatccagac cgctgccgat
     3901 gaccatgttg taggacaggt acgcattgtt ttcaagcgga tcgccgtcga gcaccgggta
     3961 ctcgtgtaca atgtcgaggg tctctgcaaa tggtttctga tacggaattt cggattttac
     4021 ttcttttgca ttaaaatggg acaggtattt ttcatccatc catgccagtt tttcttccat
     4081 atccatgttt ccgtacaggt agatatagct gttggacgga tggtaatagg tctgatggaa
     4141 attcaggaag ttttcgtggg tcaggttcgg gatgcaggac ggatctccgc cggattctac
     4201 gccgtacgga gtgtccggga acagggtgtt gaaggtttca cgctccagga catcgtctgc
     4261 ggaggagaag gctcctttca tctcgttgta tacaacaccg ttgtaggtca gcgggccgtc
     4321 ttcgctttcc agatgatagc tccagccttc ctgacggaag atttcgtcgt gtttgtaaat
     4381 gttcgggtag aatacggcgt ccatgtagac atgcatcagg ttctggaagt cggcatcgtt
     4441 ggtgctggct accgggtaca tggttttatc cgggtaggtc atagcattta agaaggtgtt
     4501 cagggatcct ttgacgagtt ctacgaaggg atcctttaac gggaattcgc gggagccgca
     4561 gaggacgctg tgctcgagga tgtgggctac gccggtgctg tcggccggcg gggtacggaa
     4621 cgcgatgttg aagactttgt tgtcatcttc gttttcgaga aggagcacgc gggcaccgct
     4681 cttgttgtgg cggaggaggt agccgtgtgc atggatatcg tcaatctgct gttcctgcag
     4741 cagggtatag gctttgcaat gttctgcgtt catttttgta tatcctttct tgtttttctt
     4801 tattatttcc ggttttatgc agtcaaaaac ggaccgcagt gcccgtttgc tgggattttc
     4861 tgcggccctg tcggttttat tcttcgttgt cggtttctgc tgcggtgttc tctgtctcgt
     4921 cgacgtgttc ttttacttcg tcgtttgcag cgttgttctg tgcatttttc tgtgcctgtg
     4981 ctgcagcggc ctgtgccatc attgcctcaa ggttcgggcg gacgctctgt gcctgggctc
     5041 ccatgattct ctggagctgt tctctggtca acagaaggtt gaagccggac gggttgatga
     5101 cgaagccttt ggcctgtttc atcaggtacg gcagaaggtg ctggaacggg atggctgcca
     5161 gacggagttt tgcctgcgga tccggacgga atttctggaa ttcaccaata tcggaaaaca
     5221 gcggctggaa gatgtcttcg ttctggtttt tgatgtacgg gatgcgcacg tctggtcccg
     5281 ggttggccgg atcgaattcg ccctgtacct gggagatgtc gatggcaagg atgaatttgc
     5341 ttcgcatcag gttgacaagc atttcttctt cgagttcacg caggtgctgc atgcgctcgg
     5401 catcgtctct tctctggttt ggttttctga gttcctgcag aaagtatacg acggtgagct
     5461 gtacgcccgg gttcagaacc gggaggtgct gtttgttcat tttctccatg tctggtttcg
     5521 ggaagatctg ctcgagctcg atgcggcggg tctggtcgcc ttcgcggaag atgaccatat
     5581 tgacgccttc ggcgtagagg cccatataga agttcggcat ctgcttgcgc aggactttta
     5641 cggtgagcag cggggtgttc ttttctacat aggattttgc aaactccttg gctgcttctt
     5701 cttcggtaaa catgtatacc tgatcatcaa aggtttccgg atcgcactct acgtatggga
     5761 gttttgtgat ctgggaaaag gtcacataga tgatttccat gctctggaat ttcttcatgg
     5821 tgaagctgtt gtctactgtc atttgtggtt gagtcctttc ttcgctctta gaggatatgg
     5881 tatccctaaa atatctatgt gacattatat catacttttg gtgggttgcc aaaggttttc
     5941 ttttggggtg acggaccgcg cagaccaaag ctgtcgaaac ggggcctgac tttgcatgac
     6001 ggcgggagag ggctttttgt agtctgtaat tggggcgtgc agactacgga aatgtttttt
     6061 aacacattct agttaatcgc tttggcattg gggtagattt tttccattcg ggcgtccggg
     6121 aaatgggcat ctatggtctg ctcgatggca ttagctgcgg gcttttggtg ggcgcgttcg
     6181 tcgtatcggg cgagagcaag ggtagatacg agcatgttgg ccaccatgaa cacgatcagg
     6241 atccaagtga tgatttctcc gaatttcatc gggatttttt caatccagcg cgaaatgaca
     6301 ggatagaatt ttttgagcca gacgacggcg gctattcccc agaagaaaca gtacaggagg
     6361 ttgatacggc ctccgaggtt gaagggaatg tcgctgtagt cccagaagac ttttccgaat
     6421 acgatttcgg tgaacacact gcagatgtat tcgtaggctc cgccgaggaa ggtaccaaag
     6481 gcaaacagga atccatcgga gcggtcgcgg tagttgtaga ggaaccaggt agcgagggcg
     6541 atggccaggc cccagacgat gctgaacggg ccccagacaa ggctgctgcg gctcatccag
     6601 acaccggcgg tgatgcggca gaatatggtt tctgtaatat cgcccaaaaa tgcgccgatg
     6661 atgaagagaa gaacgatttt gtaaaagctg catccttcgg caaatacgcc ggtttctgtt
     6721 ttctcttttt tctccggaac tggctgtacg gccggatagg cacgttccac acgacgctcc
     6781 agatagcggt aaacacggac agaaaggcgt ctggtgaagg cgtcgatctg atcgttcata
     6841 gcgctgatcc gcggcatttt tttcgtgatg tggtgcagaa cagcatagga accgacggca
     6901 tctactacca atataccaaa gagaatccag gatgtgatat gcaggaaacc ggccggaatt
     6961 aaatgaagaa ctgtaaagca gaggcgattg ccgattttca tggcaagcag tcctaaaatt
     7021 ccccagagta cagaatactg gaggcagata tagccgtcca tgttccattt tttattggaa
     7081 tagtcccacc atttgccatg gccaatgcgt tccagcgcgt gaccggccag ccactcgact
     7141 gcggtagcga taatcataca gccaaggaac aaaaagacgg ggcttgactg cagttcatac
     7201 agataacgtg tcataaagac agctgtgatt ccatagatgg cgcagagcgg accatttaag
     7261 aatcctctgt ttaccatatg ttttttccgg atcgcggcca cagaagtttc cagaagccat
     7321 ccaaggaagg cgtaaaagaa aaacaggccg agatagtcat aaattgtata gttcatgttc
     7381 ttctctctat tctttcatta tttttgctat tttaaggatt ttattttcca ctgaatccct
     7441 tgtatactct ccagtatttt gtttttattt ttatcctgca aataagatgg atttaatttt
     7501 gcttttttat actgcaaata tgttccttct gttttctgaa tcagcaattt tgtaaaatat
     7561 tgttcccagc taaaaaactc tgtgctgtcg atataatctt ctggattttc cagaatttta
     7621 cgaacctctt tgtcctgtaa aagatctgaa tttaaaatca gccattcaaa agactccggt
     7681 aaatataaat gtacgttctg tttttcctct attttattgc tgagaagatt catttctgct
     7741 ccaattgcag caccgtccgc aataatacag gtttcttctg ttcctgcctt gccgatcatt
     7801 tccggtattt ttgtttttcc accagcgctt tcacatgtaa tattcctttc tgcacataca
     7861 ttttcaaaaa attgataccc agaattggaa tcttccgtaa ttattttttg tactctaaca
     7921 ggaagccgtg ctgttttggg atagatctga tacattttct gatatatttt tctggtatct
     7981 ctgtattttc cggaaacatg caatccataa atctcttcta cactatacgg caaattatat
     8041 aagttttctc tcgtaatcaa tacaaaatag ttttctgaac cccgtatggc agaagcaaat
     8101 tcttctgttt ttataaaata attctcctcg tctatgaaaa agatgctttc ccggctgttt
     8161 tccaaaataa ttttccaatt aacaccttcc agcactctgc acggtacttc acaggaaacg
     8221 ttgattccgc tggactcccc taaattttct gcctgccgga tcatatttac cagtgttgtt
     8281 tttcctgatg cactatcccc ctggatgatt gtaatattac gctttatcac aaattcataa
     8341 tgaactctgt tattttgaat aataactctt ttctttccct tcatacatcc acctatacat
     8401 aagcctcggc aacatctaca aattcggaca tattccgcac aatagtccct gtatttaaaa
     8461 tcaggatttc aaaaggatct ttgccaaacc gcatcacatg acgcagatta atcgtcagat
     8521 ccttttgctc tcctaatttt aaaatccatt ttgcacaatt atctccacat acggaggcat
     8581 taaagatctt tccgtctgta tcgtatgcca tcagcagcaa cgttttgatt cctccggata
     8641 aatctttcgg tggaatcgga ccaagcaccg ggctgtcaat cagatgcgca ctgattacct
     8701 ccgagcgatc cacatcgcgg atcatttctt ttgccagtgg cgtggtcagc cattcatctt
     8761 cataacaatt atcaaaatag gttggcggat gatagattgc acctttcatt tctccaagat
     8821 atattttcaa cataataaaa ttcctctcgc taacgctcat tatatcgtga tacgcacatt
     8881 ataccatatc tcaaagcctt cttgcgcaaa aatgtatcgt ttgtcaacat tttccgggat
     8941 aaaaatgttg acaaacgata caaaactatt gccaaatgtc gctaattcat tttttctccc
     9001 aaaaagaaag aagccatact ctctcaagta tgacttctct cctttttcaa attcttttta
     9061 aggaaaaaga attactcttc ttctgcgccg tacagtttta cgagtttgtt caggtaagtg
     9121 taacggtctt ttg
//
LOCUS       DAOMUF010000079         9123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1933_length_9123_cov_2.973644, whole genome shotgun sequence.
ACCESSION   DAOMUF010000079 DAOMUF010000000
VERSION     DAOMUF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1933_length_9123_cov_2.973644"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="PLN77_05960"
     CDS             <1..1746
                     /locus_tag="PLN77_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM43857.1"
                     /translation="TEIMNALYTTLIIALLASAIATILGTLACIGINSMSKKSRTVFM
                     GITNIPMLNGEIVMGISLMLLFIICRIQLGFGTILMAHITFNVPYVILSVMPKLKQTN
                     KSTYEAALDLGASPLYAFWKVIFPDIMPGVVSGFLLSFTMSLDDFVITYFTKGPGVDT
                     LSTKIYAEVRKGIKPEMYSLSTILFVTVLLLLLLVNVNPSPKEEKEEAKEREAMAKKG
                     IRFRITKRVVFRRILPIAMVLVIGGGGIYYGSQNASAGGSQQLIVYNWGEYMDSDVID
                     IFEEETGIHVVYEEYETNEIMYPKIKSGAISYDLVCPSDYMIQKMIQNDLLQPINFDH
                     IPNASNIGDMYYETSEQFDPGNLYSIPYCWGTVGILYNKKMVTEPIDSWSVLWDTNYR
                     DSILMQDSVRDAFAVALKDLGYSLNSTNIKELTEAKDLLVTQKPLVQAYVVDQVRDKM
                     IGNEAAIGVIYSGEAIYTKRENPDLEYVVPKEGSNVWIDSWCIPKDAQHVENAEKFLN
                     FLCRPDIALKNFEYITYSTPNDAARELIEDDDIRNSEIAFPDEETLARCETFTYLGDD
                     ADEVYNQLWREVKSN"
     gene            complement(1846..2634)
                     /locus_tag="PLN77_05965"
     CDS             complement(1846..2634)
                     /locus_tag="PLN77_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRM43858.1"
                     /translation="MPTTYAHDRFGREVYEQLPANLKKIIRENKKLYLIGLHGPDIFF
                     YYHPFSKNRVSDYGTFLHEQTASVLFEDEVKKYQQSPSEAMEAYLLGFACHYLLDSTC
                     HPYIGKFVDHTGISHAKIETSLDQYFMLEDGLDPLVYRPASPICPHTDGNKVIHRCFP
                     EIGETEIVECLKGMKLWTRITICRSSAVRSLLLGAMKLVGCYDSMGGRVMPGRPQKEC
                     EAGTRNLVHLYERALAEAPQELVKLDEVLHGNGTLSPRFDHNFD"
     gene            complement(2668..3291)
                     /gene="scpB"
                     /locus_tag="PLN77_05970"
     CDS             complement(2668..3291)
                     /gene="scpB"
                     /locus_tag="PLN77_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRM43859.1"
                     /translation="MEEQNRNKETAPGEARRPSSVEEAEAAIEAVLFAMGDSVELGRI
                     AKAIGHDTETTRRILNHMMEKYNTKDRGIHIVELENAYQMCTKQEYYDYLVNIAMQPK
                     KAVLTDVMMETLSIIAYKQPVTKQEIDKIRGVKSDHAVNKLVEYNLVQELGRLDAPGR
                     PIVFGTTEEFLRNFGVDSTDNLPEISPVKMEDFRAEVEEEMQIKLDV"
     gene            complement(3269..4090)
                     /locus_tag="PLN77_05975"
     CDS             complement(3269..4090)
                     /locus_tag="PLN77_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRM43860.1"
                     /translation="MAIPVKLPVFEGPLDLLLHLIDKNKIDIYDIPIVEITDQYLEYI
                     HQMEREDLDIMSEFMVMAATLLDIKCRMLLPKEVNEEGEEEDPRAELVQQLLEYKMYK
                     YMSYELRDRMNEASRSVYKEPTLPREVLEYRTPVDTSELLSQISLAKLHTIYKMILKR
                     QEDKIDPIRSRFGKIEKEEVSLPDKMTYVEQFAVKKRRFSFRDLLESQPGKVHVIVTF
                     LAILELMKTGKIRISQEELFGDILIESLEDEDTVVVPYTDETDSEDKTDGRTEQE"
     gene            complement(4106..4906)
                     /locus_tag="PLN77_05980"
     CDS             complement(4106..4906)
                     /locus_tag="PLN77_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM43861.1"
                     /translation="MRKFTTTYYKLSSNGKFPEGNGFRLAFISDLHNMEFGPDNTVLK
                     AVIDEAAPDLVIIGGDQIVAKPGENLDPGLSFAERLAEEYQVIYGCGNHEYRLKLYPE
                     QYGDLYEKLRERLKKAGICYLENETADLTISGCPVRIYGYAMDRTYYNRFYRGPLPVS
                     ELTDHLGKPDPARYGILLAHHPAYRSSYASYGADLTLSGHYHGGVVGLGAHRGLVTPD
                     FRLFSKNCRGLFLTGGTCQIISAGTGEHTIPVRIWNRRELIITDIGKP"
     gene            complement(4960..5694)
                     /locus_tag="PLN77_05985"
     CDS             complement(4960..5694)
                     /locus_tag="PLN77_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HRM43862.1"
                     /translation="MAKKNYYAVKQGKVPGIYRTWDACKAQVHGYPGAIYKGFERLEE
                     AEAFLGGGKMPEPLLRETKGTYFAAPERKAVQSTAAPDPSSLDWEHLILAEKPCEDPE
                     YAGVGRSAVAYVDGSYQHALGKFSCGVVLFLDGKELNLAEEYSDPDLVSMRNVAGEIK
                     GAELAMAYCLTHDIPELAIYHDYQGIASWCTREWQAKKQGTIDYRNFYDLASAKVAIR
                     FCKVKGHTGVTYNEKADQLAKQALGL"
     gene            complement(5728..6976)
                     /locus_tag="PLN77_05990"
                     /pseudo
     CDS             complement(5728..6976)
                     /locus_tag="PLN77_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882833.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            complement(7051..>8153)
                     /gene="cls"
                     /locus_tag="PLN77_05995"
     CDS             complement(7051..>8153)
                     /gene="cls"
                     /locus_tag="PLN77_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRM43863.1"
                     /translation="TGFEYYAPCENGFDEMLKELKKAEKYIFMEYFIISEGYLWDTVL
                     EILKEKAAAGVDVRLIYDDFGCVTTLPYQYYKSLQQMGIKCAAFNQFRPVLNVILNNR
                     DHRKIAVIDGHTAFTGGINLADEYINRKERFGHWKDSGICLHGEAVWSFTVMFLQMWS
                     TITGMECNFESYRPEIYHEEAFKKDGFVQPYGDSPLDGETVGEHVYLNIISQAKKYLY
                     IFTPYLIPDNETLTALCMAAKRGVDVRIVTPGIPDKKIVYILTQANYEILLESGVQIY
                     QYTPGFIHSKSFVCDDEVATVGTINMDYRSMYLHFECGVWMYGSSAVAQVKEDALTTF
                     EQCEQINMDYCRKKNFAVRALRGALKFLAPLL"
     assembly_gap    8154..8163
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8164..8708)
                     /locus_tag="PLN77_06000"
     CDS             complement(<8164..8708)
                     /locus_tag="PLN77_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HRM43864.1"
                     /translation="MRILYKIANKLFRIVKKILKLLTSRMVIIGVALLLEFCWLAGIM
                     MQLNEKSVYINYAVTILSLIAVLSIINNPKMNPGYKLMWAVCILALPVTGICLYGILG
                     HSSVARKFRTHYDTVLLSQGGVLGEEEETRKKLEEENILAAGQSSYLTNYAHYPVYEN
                     TETEYYPLGDVWFEHFIEELK"
     gene            complement(8803..8979)
                     /gene="rpsU"
                     /locus_tag="PLN77_06005"
     CDS             complement(8803..8979)
                     /gene="rpsU"
                     /locus_tag="PLN77_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRM43865.1"
                     /translation="MSNVIVKENETLDSALRRFKRSCAKAGIQQEIRKREHYEKPSVR
                     RKKKSEAARKRKYN"
BASE COUNT         2035 a         2488 c         2088 g         2502 t
ORIGIN      
        1 acagagatca tgaacgcgct gtatacaacg ttaatcattg cgcttcttgc ctctgcgatt
       61 gcaaccatcc ttggaacgct tgcgtgcatc ggcatcaaca gcatgtcgaa aaagagccgg
      121 acggttttca tgggcatcac caatattccg atgctgaacg gtgagatcgt catgggtatc
      181 tcgctgatgc tgctctttat catctgccgc attcagcttg gattcggtac cattctgatg
      241 gcacatatta cgttcaatgt tccttacgtt atcttgagcg ttatgccgaa attaaagcag
      301 acgaacaaat cgacgtacga ggcagctctc gatcttggtg cctctccgct gtatgcattc
      361 tggaaagtta tcttcccgga catcatgccg ggtgttgttt cgggctttct gctctccttt
      421 acgatgtccc tcgatgattt cgttatcacc tacttcacca agggaccggg cgttgatacg
      481 ctctccacaa agatttatgc cgaagtccgc aagggaatca agccggagat gtactcgctc
      541 tccacaattc tttttgtcac cgtactgctt ctgctgctcc ttgtgaatgt gaatccttct
      601 ccgaaggaag aaaaagagga ggcaaaagag cgcgaggcta tggcgaagaa gggcatccgt
      661 ttccgcatca cgaaacgtgt ggtcttccgc cggattcttc cgatcgccat ggtgcttgtc
      721 atcggcggcg gcggtattta ctacggaagc cagaatgctt cagccggcgg ctcccagcag
      781 ctgattgtgt acaactgggg cgaatatatg gattctgatg tcatcgatat ctttgaggaa
      841 gaaaccggta tccacgttgt ttacgaggag tatgagacga acgaaatcat gtatccgaag
      901 atcaaatccg gtgcgatttc ctacgatctt gtctgcccgt ccgactatat gattcagaag
      961 atgatccaga acgatctgct gcagccgatc aactttgacc atattccgaa cgcttccaac
     1021 attggagata tgtactacga gacttccgag cagttcgacc cgggcaatct ctactcgatc
     1081 ccgtactgct ggggaacagt tggtattctg tacaacaaaa aaatggtaac cgagccgatc
     1141 gactcctggt ctgttctgtg ggataccaat taccgcgaca gcattctgat gcaggacagc
     1201 gtgcgcgatg cgtttgctgt tgctttaaag gatctgggat actccttaaa ctccaccaat
     1261 atcaaggagc tgacggaggc gaaggatctg cttgtgacac agaaacctct cgtgcaggcg
     1321 tacgttgtgg atcaggtgcg tgacaagatg atcggaaatg aggcggctat cggcgttatt
     1381 tactccggtg aagctatcta cacgaagcgt gaaaacccgg atctggaata cgttgttcca
     1441 aaagaaggct ccaacgtctg gatcgattcc tggtgcattc caaaagatgc gcagcatgtc
     1501 gagaacgcag agaaattcct gaatttcctc tgccggccgg acatcgcact gaaaaacttt
     1561 gagtatatta cctactctac gccgaatgat gcggcgcgtg aactgattga ggatgacgat
     1621 atccgaaaca gtgaaatcgc cttcccagat gaggaaacgc ttgcacggtg cgaaaccttt
     1681 acgtatcttg gcgatgatgc cgatgaggtt tacaaccagc tgtggcgtga agtaaaatca
     1741 aattaaagat ctgctttcgg acaaaaaaag atcccggtca ggaattctta ggaaagattc
     1801 ctgccgggat ttttctgttc ttactgttgc tctcaaaagt ctgttttaat caaaattgtg
     1861 gtcaaagcgc ggggaaagcg ttccgtttcc atggagcact tcatccagct ttacaagctc
     1921 ctgcggcgcc tctgcaagcg ctctttcgta caggtggaca agattccgtg tgcccgcttc
     1981 gcattccttc tgcggccttc caggcatcac acggccgccc atcgaatcgt agcagccgac
     2041 cagtttcatc gctccaagaa gaaggcttct taccgcactg ctgcggcaga tcgtgattct
     2101 cgtccagagc ttcatgccct tcaggcattc gacaatttct gtttccccga tttccggaaa
     2161 acagcggtgg atgaccttgt tgccgtcggt gtgcgggcag atcggcgatg ccggacggta
     2221 tacgagcgga tcgaggccgt cctccagcat gaagtactgg tcaaggctcg tctcaatctt
     2281 tgcatgggaa attccagtgt gatccacaaa tttcccgata tacggatgac acgtcgaatc
     2341 gagaaggtaa tggcaggcaa agcctaacag atacgcttcc atcgcctctg acgggctctg
     2401 ctgatacttt ttcacctcat cctcgaacag aacggaagcc gtctgctcat gcagaaacgt
     2461 tccgtaatca gacacacggt ttttcgagaa cggatggtaa taaaagaaga tatccggccc
     2521 gtgcagtccg atcagataca attttttgtt ttctcgaatg atctttttca aatttgccgg
     2581 aagctgttca tagacttctc ttccgaacct gtcgtgtgcg tacgtagtag gcatatcggt
     2641 atctcccttc ttttttctcc tgccggatca gacatccagc ttgatctgca tttcctcttc
     2701 cacttctgcg cggaagtcct ccatcttgac cggactgatc tccggcagat tgtcggtgga
     2761 atcgacgccg aagttccgca ggaattcttc ggtcgtgccg aacacaatcg ggcggcccgg
     2821 cgcgtcgagg cggccaagct cctgcaccag attgtactcc accagtttgt tgaccgcgtg
     2881 atcgctcttg acgccgcgga tcttgtcgat ctcctgcttc gtgaccggct gcttgtaggc
     2941 aatgatcgag agggtctcca tcatgacgtc ggtcagaacc gccttcttcg gctgcatggc
     3001 gatgttgaca agataatcat aatattcctg tttcgtgcac atctgatacg cattttcaag
     3061 ctccacgatg tggatgccgc ggtctttcgt attatatttt tccatcatat ggttcagaat
     3121 cctgcgggtc gtctccgtgt catggccgat cgcttttgca atccttccca gctccacgga
     3181 atctcccatc gcaaaaagga ctgcctcgat ggctgcttcc gcctcctcga ccgaagaggg
     3241 gcgtcttgcc tcacccgggg cagtttcttt attcctgttc tgttcttcca tctgtcttgt
     3301 cctcactgtc tgtctcgtct gtatacggca caactacggt gtcttcatcc tccagggatt
     3361 caatcaggat atccccgaaa agctcctcct ggctgatccg gattttgccg gttttcatca
     3421 gctccaggat tgcaaggaag gtgacaatca catgcacctt gcccggctgg gattccaaaa
     3481 ggtcgcggaa gctgaagcgg cgttttttaa cagcaaactg ctcgacatac gtcatcttgt
     3541 ccggcaggct gacctcttct ttttcgatct ttccgaacct gctccggatc gggtcgatct
     3601 tgtcctcctg gcgctttaaa atcattttat agatcgtatg gagcttcgcg agagaaatct
     3661 gcgaaaggag ctcggaggtg tcgaccggcg tcctgtactc cagcacttct cttggaagcg
     3721 tcggttcctt gtacacggaa cggctcgcct cgttcatgcg gtcacgcagc tcgtacgaca
     3781 tgtacttgta cattttgtac tctaaaagct gctgcaccag ctcggctctc gggtcctctt
     3841 cctcgccttc ctcattcact tctttcggaa gcagcatgcg gcatttgata tccagaagcg
     3901 tcgccgccat gaccatgaac tcgctcatga tgtcgaggtc ttcccgctcc atctgatgaa
     3961 tgtattccag atactggtcc gtaatctcta caatggggat atcgtaaata tctattttgt
     4021 ttttatcgat caggtgaaga agaaggtcca gcggtccttc aaacaccgga agctttactg
     4081 gtatcgccat gtgctctgtt ctcccttacg gtttcccgat atctgtaata atcaattctc
     4141 ttctgttcca gatgcggacc ggaatggtgt gttcgcccgt tcctgcactg atgatctggc
     4201 aggtgccgcc cgtcaggaac agcccgcggc agtttttcga aaaaaggcgg aaatccggcg
     4261 tcacaagccc ccggtgcgcg ccaaggccca ccacgccgcc gtgataatgg ccggaaagcg
     4321 tcagatcggc tccatatgat gcataggagc tgcggtaggc agggtgatgc gccagcagaa
     4381 tgccgtagcg cgccggatcc ggttttccaa gatggtccgt cagttccgaa accggcagcg
     4441 gtccgcggta aaagcggttg taataggtgc ggtccatggc atagccgtag atccggaccg
     4501 gacagccgct gatggtaaga tccgccgtct cattttccag atagcagatt cctgcctttt
     4561 tcaggcgctc ccggagcttt tcatacagat cgccgtactg ttccggatac agcttcagac
     4621 ggtattcgtg gtttccgcag ccatagatta cctggtattc ctccgcaaga cgttccgcga
     4681 aactcagacc cgggtcgagg ttctcccccg gctttgccac aatctggtct ccgccgatga
     4741 tgacaagatc cggagccgcc tcatcgatga ctgcctttaa caccgtgttg tccgggccga
     4801 actccatatt gtggagatca ctgataaacg ccagacgaaa accgtttcct tccggaaatt
     4861 ttccgtttga cgaaagtttg taataggtcg ttgtaaattt tctcataccg ttcattatac
     4921 ttgatttttc ggtgtatgac aacccgtttt ccctgctgtt tataagccca gtgcctgttt
     4981 tgccagctga tcggcttttt cgttgtaggt gacgccggtg tggcccttta ctttgcagaa
     5041 gcggatcgca actttggccg atgccagatc gtaaaaattc cggtaatcga tggttccctg
     5101 ttttttcgcc tgccattctc tcgtacacca gcttgcgatt ccctggtaat cgtgatagat
     5161 ggccagctcc ggaatatcat gtgtcaggca gtaagccatg gccagctctg cgcccttgat
     5221 ctcgcctgcc acattccgca tggagacgag atccggatcg ctgtattctt ctgcaagatt
     5281 cagctctttt ccgtccaaaa agaggaccac accgcaggag aactttccga gcgcatgctg
     5341 atagcttcca tccacgtatg cgacggcgct cctgccgaca ccggcgtatt ccgggtcttc
     5401 gcatggtttt tccgccagaa tcaggtgttc ccagtccaaa gaagaaggat cgggtgctgc
     5461 cgtgctctgt actgcctttc gttccggggc agcgaaatac gttccttttg tttcccgcag
     5521 caatggttcc ggcattttac cgccgcccag aaaggcctct gcctcctcca gacgctcaaa
     5581 tcccttgtaa attgcgccag gataaccgtg tacctgcgct ttgcacgcat cccatgtccg
     5641 gtagatgccc ggaacctttc cctgttttac cgcatagtaa tttttctttg ccatgttttt
     5701 ctccttttcg cgcatgatac tccggcttta tgctgtccca agaaaatatt gtttcatcgt
     5761 acatccgaac agatcccaca tgccggctct tgccacgtct gctccatata aaatcgcaac
     5821 ggaaccaagt tcctcaccgt ctgctttgta aacaactctt ccggcacact cgcctttctg
     5881 gataggggcc tgcacctctt ccggcagttc catcgttttt tccacttcac caatcaccgt
     5941 cccgcccgtc gtaagatagc ggaattcttc cgcatattcc agttctgccg tatcctgctt
     6001 tccgtaaaca accgaaagtt ccggcagggg atcgcgttcc cggtctatgt agatgctgca
     6061 ggacgaaaac ccatactgga acagtgacgc tgcatctgca aaccgcacct tgtagtccgg
     6121 tgctcccatc acgactgcca gcatcgtcag atcatttttc ttcgccactg ccgagacgca
     6181 gtacttcgcc ttcgaggtgc ttcctgtttt cagtcccaca cagccctcat agccgcggat
     6241 cagcttattt gtattggtga gggtgaatgg tttgcttccc ttctgtgtga catgcgtaat
     6301 ctcttccatc cagatagagg aatagttcga tcagctcccg agacattttc gccacatctg
     6361 ccgctgtcgt gtaatggccg tcggaatccg tgaggccgca gcagtcctca aacttcgtgt
     6421 cgttcatgcc gagttctttt gctttctggt tcatccggct gacgaactcg ctttctgtcc
     6481 ccgcaatata ctctgccatc gctacagatg catcgtttcc cgaagcaacg acaatacact
     6541 tgatcatcgt ctcaacggtc tgcttttcgc cctcttccag aaaaacctgg gacccgccca
     6601 tacttctggc gtgcgcactc gtcgtcactt cgtcggtcag ctggagtgtc cctttttcga
     6661 gctcttcaaa aatcagcagc agcgtcatga tttttgtgat gctcgccggg cttcgctgtt
     6721 ccgacgcatt tttctcgtac agaacagttc cggtctgtgc ctccatcaaa agaacagacg
     6781 gcgaagccag cccaaggtcc gttgtcatct ccggtgctgt ctcctccgtc tcttctgttt
     6841 tctgttccgt tgtttcgttc tgctgcttgt cggaagattc tccattctgc tctgccgcag
     6901 tcactttcaa gggcgctgta agaagcgcca ggatcatcag caggctccag acgatatttc
     6961 gtgttcttct cttcatattc ccccgaaaca tctgctcttt cttccctaca ggctatgcag
     7021 acgaccgcag aactatgctg tctgttccgt ttacagcagc ggtgccagaa acttcagcgc
     7081 accgcggagt gcacggactg caaaattttt ctttcggcag tagtccatat tgatctgctc
     7141 acactgttca aacgtggtca gcgcatcctc ttttacctgg gcaaccgcac tgctgccgta
     7201 catccagaca ccgcactcaa aatgcaggta catgctccgg taatccatgt tgatcgttcc
     7261 gaccgtcgca acttcatcat cacagacaaa gctcttggaa tggataaagc ccggcgtata
     7321 ctggtaaatc tgaacgccgg attccagcag gatctcgtaa ttggcctgcg tcagaatata
     7381 gacaatcttc ttatccggga tgcccggcgt tacaatccgc acatcgactc cgcgctttgc
     7441 cgccatgcag agagcggtca gcgtctcgtt gtccggaatc agatacgggg taaaaatata
     7501 aagatatttt ttcgcctgtg aaataatgtt cagataaaca tgctcgccta ctgtctctcc
     7561 atccagcggg gaatcgccgt acggctgcac aaaaccgtct tttttaaacg cttcctcgtg
     7621 atagatctcc ggccggtatg attcgaaatt gcattccata ccggtgattg tgctccacat
     7681 ctgcagaaac atcaccgtaa aactccatac cgcctcacca tgcagacaga ttccggaatc
     7741 cttccagtgg ccgaaccgct ccttgcggtt aatgtattcg tccgcaagat taatgccgcc
     7801 cgtgaaagcc gtatgaccgt cgatcacggc aattttccgg tggtcacggt tatttaaaat
     7861 gacattcagc accggtcgga actgattgaa cgccgcacac ttgatgccca tctgctgcag
     7921 cgatttataa tactgatacg gcagtgtcgt cacgcagccg aaatcatcgt aaatcagccg
     7981 cacatccaca ccggcggccg ctttttcctt cagaatctcc agcaccgtat cccacagata
     8041 cccctcgctg atgatgaaat attccataaa aatatatttt tccgcttttt ttagctcttt
     8101 taacatctca tcaaaaccat tttcacatgg agcataatat tcaaaaccag tatnnnnnnn
     8161 nnntttttga gttcctcaat aaagtgctca aaccatacat ctcccagcgg gtaatactct
     8221 gtttctgtat tttcataaac aggataatgc gcataattgg tcagatagga agactgcccc
     8281 gctgccagga tattttcttc ctcgagcttt ttacgcgtct cttcttcctc tccaagaacg
     8341 cctccctgcg aaagcagaac tgtatcgtaa tgtgtacgga attttcttgc cacactggaa
     8401 tggccgagaa tgccgtacag gcagattccg gttaccggaa gcgccaggat acagactgcc
     8461 cacatcagtt tgtagccggg attcattttc ggattattga taatcgaaag cactgcaatc
     8521 agggaaagga tcgtgaccgc atagttgatg tagacagatt tttcattgag ctgcatcata
     8581 attccggcca gccagcaaaa ttccagaaga agtgcaacac cgataatgac catacggctg
     8641 gtaagcagct tcagaatctt tttcacgata cgaaacagct tgtttgctat tttgtacaaa
     8701 attctcattt gtcaccgcct tgctgttttg tcaaaacctg cgactttaag ttacaagtat
     8761 aaaaatcccc ggcaaaacag atcgccgggg attttttgca cattaattat acttacgttt
     8821 tctggcagct tcagatttct ttttacgacg aacacttggt ttctcgtaat gctctctttt
     8881 acgaatctcc tgctgaatgc cagcttttgc acagctacgc ttaaatctgc gtaaagcgct
     8941 gtccaacgtt tcgttttctt taacgattac atttgacata gtctcacacc taacctcccc
     9001 tccagccttg tattgtgcat catacgactg ttcgggtata tttattgcac tggatattat
     9061 tataacatat tttttcaaaa cgtcaactgc tttttatgat ttttaccgat ttttttatac
     9121 ttt
//
LOCUS       DAOMUF010000080         9101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1936_length_9101_cov_3.055605, whole genome shotgun sequence.
ACCESSION   DAOMUF010000080 DAOMUF010000000
VERSION     DAOMUF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1936_length_9101_cov_3.055605"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLN77_06010"
     CDS             complement(<1..344)
                     /locus_tag="PLN77_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM43866.1"
                     /translation="MKKVTLQEIANSLGISRTTVWKVFSGHEGVSDSLRTKVIAKAQE
                     LGYAIPEDFQYPSSAEETTPVNIAVAVCRPETSLFWMTIIHQIAKVFSTHNVNLVYTY
                     LPTSADKTYFLPP"
     gene            complement(396..1463)
                     /locus_tag="PLN77_06015"
     CDS             complement(396..1463)
                     /locus_tag="PLN77_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HRM43867.1"
                     /translation="MSKNITIPVPAVPFAPPVYTCHRAKKEFHLDGNINKPFWEDAPY
                     TDEFLDIQGSHMPLPRFVTRAKMLWDDENLYVAADLAGDEIWGHLTERDSVIYFDNDF
                     EIFVDPDSDTYHYYEFEMNVLNTVWDLFLAEPYRDGGSGLNGYDMHGLRTAVHVDGSI
                     NDPSAENKGWSVEVVIPFSALYEYQKERRAPKNGEFYRMNFSRVQWNVDVKDNTYVKR
                     TDENGNVLPEDNWVWAPTGVINIHYPELWAFVFFSEDREDAPCDSTIPEDEYRKWELR
                     KLYYAENILFETTGSYSSSLDELQKTLDAYAPNDWNKSVKDLGYTIETTSRTYLISCP
                     SADRAHLLLLYSNGKVEKITL"
     gene            1706..3937
                     /locus_tag="PLN77_06020"
     CDS             1706..3937
                     /locus_tag="PLN77_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM43868.1"
                     /translation="MEAYQNTALSPEERAKDLLGRMTLKEKVGQLNQRLYGFRIYERQ
                     GEEFTLTDEFKEEVERMGGLYGLYRADPWADKDEKTGIVPELSAKAYNMVQHYVMEHS
                     RFGIPMMMSSECPHGHQALGGGLLPVNLAAGATFDPALLSEGYKACGKQLKSGHVELA
                     LMSALDMARDPRWGRSEECYSEDPCLAAAMAKAAVTGMQSTGVGSVAKHFCAQGETTG
                     GVNASAARIGERELREIHFPSAKACCEAGVEGIMAAYNEIDGVYCHRNAWLLRDVLRG
                     EMGFDGIVMADGLAVDFLKNTEGDTLHAAVAARKAGVDVSLWDEAFGRLGEAVDQGLL
                     EESQIDEAVLRVLKLKFEKGLFEHPYMEENMLSPEEAGIPEVSLALARESAVLLKNEK
                     TVLPLAKKYKKVAVIGYHAADRYCMLGDYTPPVPESECVTVLQGMKQEAPDGVKVSYA
                     MGSGFSEAEEREKAKAMALAEESDVIVAVVGGSSSRFGGAVFDANGAASKGTGSRSMD
                     CGEGMDTAKVHIPAAQEELIAALACLGKPLVTVVIAGRAYCIEKIENASDAVLYAFYP
                     GPMGGRAVAELLYGSSNPSGRLPVSMPRHVGQIPVCYNYRTSVAPAYCDMTSQPLHTF
                     GEGRSYTTFTGSDVSVTKEENGISVSFTVENTGNMAGTSVPCLYVRKRSGGVVARIKE
                     LKAFTRIALAPGEKKEAVFHLTKEELNFVQIPGKPEVICHEYELMLEDGAEKWWTGNV
                     TNL"
     gene            4388..5323
                     /locus_tag="PLN77_06025"
     CDS             4388..5323
                     /locus_tag="PLN77_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM43869.1"
                     /translation="MKEKPTKAKKKGRTIRAFKRDISLWLFCVPGVILTFIFSYIPMY
                     GVQIAFRRFNATAGITKSPWVGMYYFERFFNSPYFASTIKNTLILSLYGLVVSFPIPI
                     ILALMLNSFRHKRYRKVIQTVTYAPNFISTVVMCGMIILFLSPSVGVINHVLEAIGIP
                     AVNFMAKKAYWRHIYVWTGVWQSTGWSSVIYFAALAGISPELHEAAKVDGATKLQLIR
                     HIDLPLIMPTATILLIMNCGSILSVGFEKAFLLQNNLNASVSEIISTYVYKVGLINND
                     MSYSSAIGLFNTIVNMVMLIIVNTISDKLSGNSLW"
     gene            5338..6267
                     /locus_tag="PLN77_06030"
     CDS             5338..6267
                     /locus_tag="PLN77_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM43870.1"
                     /translation="MSKENTAVTKKKIKRCKEDVVFDAACFIILTLVLFVVAYPLYWV
                     IISSISAPSAVSSGKVLLHPIGFTLKGYAEVFKNDQVMRGFLNSLIITFCGVMVNLIV
                     TLPTSYALSRSNFSGKKPITIFYLITMFFGGGLIPTYLVIQRLQMLNTIWALILPGCL
                     SVYNMIVARTFFKSNISEELYEAAEIDGCTQARFFFQIALPLSKAIIAILVLYYGVGH
                     WNSYFSALVYISDKAKYPLQLVLRNILITNQTALSQTATTAAARAALQEKQQLIDVMK
                     YSLIIISSIPVLVMYPLVQKHFVKGVMIGSMKG"
     gene            6405..8045
                     /locus_tag="PLN77_06035"
     CDS             6405..8045
                     /locus_tag="PLN77_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM43871.1"
                     /translation="MKRKAVAAVLTAVMVASMFTGCGSDNGSSSAKSASAASAASAAN
                     DTVDEDGKVNGIMYQEGLPLVDEGDYTFSIFCDDSSESGEFIMLDEFKKQTNVDVDLK
                     IYPYETATERLNLDLNSGDYADVIGGWTLSDNAILTYGVNQGVFIPLEDYFEKYCPNI
                     SAILDLPGVREKMTAPDGHIYTIPYVCTDTTVGYSPYINTKWLENVGMSMPTTTDEFE
                     AVLKAFKEQDANGNGDASDEIPFSTDPNNKHIEAMAGYFGLPMNKLGIAIQNEKVVYG
                     GVSDTYREFLSWFHKLYAEGLVDVELYTQDSSTWEGKGNQDLYGVSIAYGSNEFTGIP
                     AAEERGDFDSMPVLNTDKDGIWLRDTEGFSVYRTQAVITNKAEHPEIICRWFDNAFAL
                     ENGIGCNRGPVGVIVNKEDDGYHAIDTTTLSEEDQEKYSWGNLWPQSLPKYLPADFEF
                     VEEHPMYDEKKATEEAYEANLTKEIIPSYWIDLDKIDTFSDTNTAIKDFFEQQQAQFV
                     CGELDIDNDADWQAYVDGMYSLGLEDWVATQGIEEIAK"
     gene            8239..>9101
                     /locus_tag="PLN77_06040"
     CDS             8239..>9101
                     /locus_tag="PLN77_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRM43872.1"
                     /translation="MMQDWFRNAKLGIFIHYGIYAVGDVSESWSFHNGNISYEDYMKQ
                     CEGFTASKFDAKKWAELFQKAGAQYVVMTAKHHDGVALFDTGYSDLSVVKKTPAARDL
                     VKEYTAAMREAGLKVGLYYSLIDWSDPRYRTVYPEGMKKEDCLNDIYGSPAGKEEEPE
                     KWQEFLEFNNHQLKELMSNYGTVDLLWFDGDWERSAAQWKMPEFREYLHTLNPNVVLN
                     SRMQGYGDYETPEQGIPLYGPKGEWEFCTTINDSWGYRPSDNDYKSSGQIVRMFCDCI
                     TLGGRMLLDIGP"
BASE COUNT         2581 a         1988 c         2410 g         2122 t
ORIGIN      
        1 ggcgggagaa aatacgtttt atccgccgaa gtcggaagat acgtgtaaac gagattgaca
       61 ttgtgtgttg aaaatacttt tgcaatctgg tggatgatgg tcatccagaa aagtgaggtt
      121 tccggtctgc agacggcaac tgcaatgttg accggcgtgg tctcctccgc gctggaagga
      181 tattgaaaat cttccgggat ggcatagcca agctcctggg ctttggcgat gaccttggtg
      241 cgcagggaat cggagacacc ttcgtgtccg ctgaacacct tccagacggt ggtccgggag
      301 atgcccaggg aatttgcaat ttcctgtaaa gtgacttttt tcatagtttc aaccgatcct
      361 gttacaaatt ttaactacta accgatatta tacgcttaaa gtgtaatttt ttcaaccttt
      421 ccgttggaat acagaaggag gagatgtgca cgatcggcac tcggacagga aatcaggtac
      481 gtacggctgg tggtttcaat ggtatatcca agatctttta cagatttatt ccagtcgttc
      541 ggtgcatacg catccagcgt tttctgcagt tcatcgaggc tgctgctgta ggagccggtt
      601 gtctcaaaca gaatattttc tgcgtaatac agcttgcgga gctcccattt gcgatactcg
      661 tcctccggaa tggtgctgtc gcatggagca tcctcgcggt cctcggagaa gaagacgaaa
      721 gcccacagct ccggataatg aatattgatg acgccggtcg gagcccatac ccagttgtct
      781 tccgggagaa catttccgtt ttcatcggtg cgttttacgt aagtgttgtc cttgacgtcc
      841 acattccact gcacacggga gaaattcata cggtaaaatt ctccgttttt cggtgcgcgg
      901 cgctctttct ggtattcgta gagagcagag aacggaatca caacctcaac actccagcct
      961 ttgttttcag cggaaggatc gttgattgaa ccgtccacat ggacagcggt gcgaaggccg
     1021 tgcatatcgt agccattgag gccgctgccg ccgtcgcggt acggctctgc gagaaacaga
     1081 tcccataccg tgttcagaac gttcatttca aattcatagt aatggtacgt atcggaatcc
     1141 gggtcaacga agatctcgaa atcgttgtca aagtagatga cagaatcgcg ttctgttaaa
     1201 tgaccccaga tttcatcgcc ggcaaggtcg gctgcaacgt aaagattttc atcatcccag
     1261 agcatttttg cgcgggtgac aaaacgcgga agcggcatgt ggctgccctg gatatccaga
     1321 aattcatcgg tatacggtgc gtcctcccag aatggtttgt tgatatttcc gtccagatgg
     1381 aactcttttt ttgcgcggtg gcaggtataa actggcggtg caaacgggac agccggaacc
     1441 ggaatcgtga tgtttttgct cataagtgaa aacctccctt tgtaatttgc aaaatttagt
     1501 agcgaaaggt atcataacac taaaagtaat aaatttcaac atgaaaaatg aacaaaaatg
     1561 aacgaaatat aacttattgt ggacaaaagt aaaaaaatga gatacaataa agccgtttca
     1621 taagagaatt ttgggggaaa cagatcaaaa atacaccggc aggaagaaaa cggccgggca
     1681 gaaagcatat aaggaggaaa atcagatgga agcatatcag aatacagccc tttcaccgga
     1741 agagagagca aaggatcttc tgggaaggat gacattaaag gaaaaagtgg gacagctcaa
     1801 tcagcgtctg tacggattcc ggatctatga gcggcaggga gaggaattta ctctgacaga
     1861 cgaattcaaa gaagaagtag agcgcatggg gggtctgtac ggcctgtacc gtgccgaccc
     1921 gtgggcagat aaggacgaga agaccggaat cgtgccggaa ctctccgcga aagcctacaa
     1981 tatggtacag cactacgtca tggaacattc ccgctttggc attccgatga tgatgagcag
     2041 cgagtgcccg catggccatc aggcactcgg aggaggtctg cttccggtca acctggctgc
     2101 cggagcaacg tttgatccgg cgcttctttc ggaaggctac aaggcatgcg gaaagcagtt
     2161 aaagagcggt catgtggagc tggcgctgat gtcggcactt gatatggcgc gtgatccgag
     2221 atggggaaga agtgaagagt gctactctga ggacccatgc cttgcagccg cgatggcaaa
     2281 agcagccgtg accggcatgc agtcgaccgg cgtcggcagc gtggcaaaac atttctgcgc
     2341 ccagggcgag acgaccggag gtgtgaatgc gagtgcggcg agaatcgggg agcgtgagct
     2401 gcgtgaaatc cattttccgt ctgcaaaagc gtgctgcgaa gccggtgtgg aaggaatcat
     2461 ggcggcatat aacgagatcg acggcgtgta ctgccacaga aatgcatggc tgcttcgcga
     2521 tgtactgcgc ggtgagatgg gctttgacgg cattgtgatg gcagacggac tggctgtcga
     2581 tttcctgaaa aacacagagg gggatacgct gcatgcagcc gttgcggcaa gaaaggccgg
     2641 tgttgatgtg agcctgtggg atgaagcctt tggccgtctc ggtgaggcgg ttgatcaggg
     2701 acttctggag gagagccaga tcgatgaggc ggtgctccgc gttctgaaac tgaaatttga
     2761 aaaaggtctg ttcgagcatc cttatatgga agaaaacatg ctttccccgg aagaagccgg
     2821 catcccggag gtatctcttg cactggcgcg ggagtctgct gtgcttctga aaaatgaaaa
     2881 gacggtgctt ccgcttgcaa agaaatataa aaaagtagca gtgatcggct accatgcggc
     2941 agaccgttac tgtatgctcg gcgattatac tccgccggtg ccggaaagcg agtgcgtcac
     3001 ggttctgcag ggcatgaagc aggaagcacc ggatggcgtg aaagtctctt atgcgatggg
     3061 gtccggtttt tccgaggcgg aggagcgtga aaaagcaaaa gcgatggcac ttgcagaaga
     3121 aagcgatgtg atcgtggccg ttgtcggcgg ttcctcctca cggtttggag gagctgtgtt
     3181 cgatgcaaac ggtgcagcat ccaagggaac cggcagccgt tcgatggact gcggcgaggg
     3241 aatggacacc gcgaaggttc acattccggc cgcgcaggaa gaactgattg cagcacttgc
     3301 ctgccttggc aaaccactgg taacggttgt aattgccgga agagcatact gtatcgagaa
     3361 gatcgaaaat gcttccgatg ccgttttata tgcattctac ccgggaccga tgggaggcag
     3421 ggcagttgct gagcttctct atggaagcag caatccgtcc ggccgtcttc cggtttctat
     3481 gccacgccac gtgggacaga tcccagtatg ctacaattat cggacttccg ttgctccggc
     3541 gtactgtgat atgacaagcc agccgctgca taccttcgga gaaggaagat cctacacaac
     3601 gtttaccggc agtgatgttt ccgtaacaaa agaagaaaat ggtatttctg tttcctttac
     3661 cgtggaaaat accggaaata tggccggaac ttccgttccg tgcctgtatg tgcggaaacg
     3721 ttccggcggc gttgtggcaa gaatcaaaga gctgaaagcc tttacgagaa ttgcacttgc
     3781 accgggagag aaaaaagaag ctgtgttcca tcttacaaaa gaggagctga attttgtcca
     3841 gattccggga aaaccagagg tcatctgcca tgagtacgag ctgatgcttg aagatggagc
     3901 agagaaatgg tggaccggaa acgtgacgaa cctgtaagag ctgacgaaaa aacagtgtaa
     3961 tcaaaatgaa caaagaagga aactatatta caaaaataaa gaaacatagt ttccttcttt
     4021 ttgtttacaa aaaatggata aaaatataaa tggatcgtat gctctgacgc cgacaaaaga
     4081 aaaataaagg gaaaaacagg aggatttatg ggaaaagtgc ttaaaaagta tcggtttaaa
     4141 ttgcgtaaaa gtaacaaaat aatatttgtg ttcaaaaatt aattgacaaa taaggaaaca
     4201 aaatgtaaaa taaattcatc aaaacagtgt tggataaatc ttaaatcaca gatttgattg
     4261 tggcagttgc acaaaaaaga agcagcatgc gaggatgatg cgaaagcaaa atgttcaaaa
     4321 tgttcacaac aagggatgct ccaatcaagg aaaacacgaa acctttaaaa gggaggacaa
     4381 gagagaaatg aaagagaaac caacaaaagc aaagaaaaaa ggccgcacca tccgcgcctt
     4441 taagagagac atctcgcttt ggcttttctg tgttccgggc gtgatcctga cattcatctt
     4501 cagctacatt ccgatgtatg gtgtacagat cgcgttccgc cgctttaatg cgacagccgg
     4561 aatcacaaag agcccgtggg taggcatgta ctactttgag agattcttca actctccata
     4621 ctttgcatcc accatcaaga acacgttgat cctgagtctg tacggactgg tcgtatcatt
     4681 cccgattccg atcatcctgg cactgatgct gaactccttc cgccataaga gataccggaa
     4741 agtgatccag accgtcacct acgcgccgaa cttcatttcc accgtagtta tgtgtggtat
     4801 gatcattctg ttcctgtctc cgtccgtcgg tgttatcaac cacgtactgg aagcaatcgg
     4861 aattccggcg gtcaacttca tggcgaaaaa agcctactgg agacacattt atgtgtggac
     4921 cggcgtatgg cagagtacag gatggagctc tgttatttac ttcgcagcac tcgccggcat
     4981 cagcccggag ctgcatgagg cggcaaaggt agacggcgcg acgaaattac agctgatccg
     5041 tcacatcgac cttccgctga tcatgccgac cgcgaccatt cttctgatta tgaactgcgg
     5101 aagcattctt tccgtcggct tcgaaaaagc cttccttctg cagaacaacc tgaatgccag
     5161 cgtatccgag atcatttcca cctacgtcta taaggtcggt ctgatcaaca acgatatgtc
     5221 gtattcttca gcaatcggtc tgttcaacac catcgtaaac atggttatgc tgatcattgt
     5281 caatacgata tctgataaac tttcgggcaa cagcctgtgg taggaggaaa caaaaacatg
     5341 agtaaagaaa ataccgcagt gacaaaaaag aaaataaaac gctgcaaaga ggacgtcgtt
     5401 tttgacgcag cctgcttcat tatcctgacg ctggttctgt ttgttgtggc atatccgctg
     5461 tactgggtta tcatttcatc catcagtgcg ccaagcgccg tatcgagtgg taaagtactg
     5521 cttcacccga tcggatttac cctgaagggc tacgcagaag tatttaaaaa cgaccaggtt
     5581 atgagaggct ttttaaactc cctgatcatc accttctgcg gtgtcatggt caacctgatc
     5641 gtaacgcttc cgacatcata tgcactttcc agaagcaatt tctccggaaa gaaaccgatc
     5701 accatttttt acctgatcac catgttcttc ggcggcggtc tgatccctac ctatctggtt
     5761 attcagagac tgcagatgct gaacacgatc tgggcactga ttcttccggg atgcttaagc
     5821 gtatacaaca tgatcgtggc aagaaccttc ttcaagtcga acatttcgga agagctgtat
     5881 gaggcagctg aaattgacgg ctgtacccag gcgagattct ttttccagat tgcacttccg
     5941 ctttccaaag cgatcatcgc aattctggtt ctgtactatg gtgtcggtca ctggaactca
     6001 tacttctcag cactcgttta tatttcggac aaagcaaagt atccgttgca gctggttctg
     6061 cgtaacatcc tgatcacgaa ccagaccgcg ctgtcccaga cagcaacaac tgcggcagca
     6121 agagcagcac tgcaggaaaa acagcagctg atcgacgtta tgaaatactc gctgatcatc
     6181 atcagcagta tcccggttct ggttatgtac ccactggtgc agaagcactt tgtaaagggt
     6241 gttatgatcg gatccatgaa gggttgattt acaagaagaa gtttttttaa cgatttatct
     6301 gccgcacggg aaacagcggt atgtaaataa agggtccgga caacggacag gcaaaagcgc
     6361 ttccggcgga aaatgccgcc ggaacaacac aggagggaaa aatcatgaaa agaaaagcag
     6421 tcgcagcagt tttaactgca gtaatggtgg catctatgtt taccggatgt ggaagtgata
     6481 atggaagcag ctcggcaaag agtgcttctg cagcatccgc agcatccgca gcaaacgaca
     6541 ccgtggatga ggacggaaaa gtaaacggca ttatgtatca ggaaggtctt ccgctggtag
     6601 acgagggaga ttacaccttc tccatcttct gcgatgattc cagcgaatcc ggcgagttta
     6661 tcatgctcga tgagttcaaa aaacagacca atgtagacgt tgacctgaaa atttatccat
     6721 atgaaacagc aacagagaga ctgaatctgg acctgaactc cggagattac gcagatgtga
     6781 tcggcggatg gaccttaagc gacaatgcaa tcctgaccta tggtgtaaac cagggcgtat
     6841 tcattccgct ggaagattat tttgagaaat attgcccgaa catttctgcg atcctggatc
     6901 tgccgggtgt aagagaaaaa atgacagcac cggacggaca catctacaca attccgtacg
     6961 tatgtaccga tacaaccgtt ggatacagcc catacatcaa tacgaaatgg ctggagaatg
     7021 ttggcatgag catgccgacc actacagacg agtttgaggc agtcttgaaa gccttcaaag
     7081 agcaggatgc aaacggcaac ggtgacgctt ccgatgaaat cccgttctct accgacccga
     7141 acaacaaaca tatcgaggct atggccggtt acttcggact tccgatgaac aaacttggta
     7201 ttgcaatcca gaatgaaaag gttgtttacg gcggtgtcag cgatacatac cgtgagttcc
     7261 tttcctggtt ccacaaactg tatgcagaag gactcgtaga cgtagagctt tacacacagg
     7321 acagctccac atgggaaggt aaaggaaatc aggacctgta cggcgtttcc atcgcatacg
     7381 gaagcaacga gtttaccgga atcccggctg cagaggaaag aggcgatttc gactccatgc
     7441 ctgttctgaa cacagacaaa gacggaatct ggctgcgcga tacggaaggc ttcagcgtat
     7501 acagaaccca ggctgtcatc acaaacaagg cagagcatcc ggaaatcatc tgccgctggt
     7561 ttgacaatgc attcgcactg gagaacggca tcggctgcaa cagaggaccg gttggtgtga
     7621 ttgtaaacaa agaggatgac ggataccatg cgattgacac caccacactg tctgaggaag
     7681 atcaggaaaa atattcctgg ggcaatctgt ggccgcagtc tctgccgaaa tatctgccgg
     7741 cagacttcga gtttgtagaa gagcatccga tgtatgatga gaaaaaagca acagaggaag
     7801 cttatgaggc aaatctgacc aaagagatta ttccgtccta ctggattgac ctggataaga
     7861 ttgatacctt ctctgatacg aacacagcaa tcaaagattt ctttgagcag cagcaggcgc
     7921 agttcgtatg cggcgagctt gacattgaca acgatgctga ctggcaggct tatgtagatg
     7981 gtatgtactc tctgggactt gaggactggg tagcaactca gggtatcgag gaaatcgcga
     8041 aataaacagg aaaaggtaac gatatttcct gtggcgtgaa aaaaggcata ttacagtgta
     8101 aaacggtaat atgcctttct ttttgggcaa gaactgatat aatgggaaga aacaataaag
     8161 gggtaattag atcggataaa aaagctatgc ccggagtctt ttaatccggg agtacaaaaa
     8221 aaggaggagc tggaagatat gatgcaggac tggtttcgga acgcgaagct gggcattttt
     8281 atccattatg ggatttatgc ggtgggtgat gtgtcggagt catggtcgtt ccacaacgga
     8341 aatatttctt atgaagatta catgaaacag tgtgaaggct ttacagcatc gaagtttgat
     8401 gcgaaaaaat gggcggagct gtttcaaaaa gcaggtgcgc agtacgtggt tatgacagca
     8461 aagcaccatg acggcgtggc actgtttgat accggataca gtgatttaag tgttgtgaag
     8521 aaaacaccgg cagcgcgcga cctggtaaaa gagtatacag ctgccatgcg tgaggcaggt
     8581 ctgaaggtcg gcctttatta ttcgctgatc gactggtcgg atccgcggta ccgcacggtt
     8641 tacccggaag gaatgaaaaa agaagactgt ctgaacgata tctatggttc cccggccgga
     8701 aaagaggagg agccggagaa atggcaggag tttctggaat tcaacaatca tcagttaaaa
     8761 gaactgatgt caaattacgg aacggtggat cttttatggt ttgatggtga ctgggaacgg
     8821 agcgcggcac agtggaagat gccggaattc agggaatatc tgcataccct gaatccgaac
     8881 gttgtattga attcgagaat gcagggctat ggagattatg agacaccgga gcagggaatt
     8941 ccgctgtacg gaccgaaagg tgaatgggaa ttctgtacga caatcaacga ttcctgggga
     9001 tatcgtccat cggacaatga ttacaagagc agcggtcaga ttgtgcgcat gttctgtgac
     9061 tgtattaccc tcggcggaag aatgctgctg gatatcggac c
//
LOCUS       DAOMUF010000081         9077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_1944_length_9077_cov_3.106850, whole genome shotgun sequence.
ACCESSION   DAOMUF010000081 DAOMUF010000000
VERSION     DAOMUF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1944_length_9077_cov_3.106850"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1232
                     /locus_tag="PLN77_06045"
     CDS             3..1232
                     /locus_tag="PLN77_06045"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRM43873.1"
                     /translation="MYKVLKSQQEEMLPKLGARVELKKILVRNVEKAAAKVDDPSIIT
                     TSFEEIANDPEIEIVVEVMGGKQPAMNYILACLDAGKNVVTANKDLVASEGKKILATA
                     KRNGKDFLYEASVAGGIPIIRPVKQCLAANHITELMGIFNGTTNFILTKMSQEGMEFG
                     DALRLAQDMGYAEADPTADVEGLDAGRKVAILASAAFNSRVTFEDVYTEGITKITATD
                     IEYAKEMGCTIKLLGVAKDTSEGIEARVHPMLIDSNHPLATVNDSYNAVFLTGDAVQD
                     TMFYGRGAGELPTASAVVGDIFDVVRNILWNCCGRIGCTCYRECPIKRVGEIKSKYFV
                     RMQIENRYGTLASVASVFGNNKVSIAQMLQKRVNGKYAEIVVITDEVKERHFEDAMQI
                     LGGMSMIQEISSTIRVY"
     gene            1414..2955
                     /gene="gpmI"
                     /locus_tag="PLN77_06050"
     CDS             1414..2955
                     /gene="gpmI"
                     /locus_tag="PLN77_06050"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRM43874.1"
                     /translation="MSKKPTVLMILDGYGLNDKCEHNAVCEAKTPIMDQLMSQCPYVK
                     GQASGLAVGLPDGQMGNSEVGHLNMGAGRVVYQELTRITKAIQDGDFFENEELVKAVE
                     NAKANNSALHLFGLLSDGGVHSHITHVFGLLELAKRKGLEKVYVHCFLDGRDTPPASG
                     KSYVEQLQAKMDEIGVGEIGVISGRYYAMDRDNRWDRVELAYKALTKGEGVEGTDAAE
                     AVQASYDAEKTDEFVLPTVLKKDGKPVATIQDKDSVIFFNFRPDRARELTRAFCADEF
                     TGFEREKRLDLTYVCFTEYDETIPNKSVAFKKEEITNTFGEYLAAHNMTQARIAETEK
                     YAHVTFFFNGGVEEPNKGEDRILVKSPKVATYDLKPEMSAYEVCDKLTDAIRSQKYDV
                     IIINFANPDMVGHTGVESAAIQAVEAVDECVGKAVDALKEVDGQMFICADHGNAEQLV
                     DYETGEPYTAHTTNPVPFILVNADPSYTLREGGCLADIAPTLIELMGMEQPKEMTGKS
                     LLIKK"
     gene            3509..4852
                     /gene="eno"
                     /locus_tag="PLN77_06055"
     CDS             3509..4852
                     /gene="eno"
                     /locus_tag="PLN77_06055"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRM43875.1"
                     /translation="MFRYLPIRSVTGREILDSRGNPTVEVEVAVGEGILGLEGHMGRA
                     QVPSGASTGKFEALEKRDQEVRYLGQGVRKAVEAVNTVLADAVVGENALEQERIDHIL
                     CQADGTETKENLGANAILGVSLAVARAAANALHLPLYQYLGGCHTSRMPVPMMNILNG
                     GRHADNTVDLQEFMIMPCGAPSFREGLSMCAEIYHTLKQLLKEKGYSTSVGDEGGFAP
                     DLKDSDEALQLIVNAVQKAGYAPGNDVMIAIDAAASELYEESRNVYYFPGESRQCGKT
                     VFRDTAEMVAYYEELIEKYPIVSIEDGLCEDDFEGWKQMTEALGNRVQLVGDDLFVTN
                     VRRLSCGIALGTANAVLIKVNQIGTLSEALDAVEMAQRAGYRAVISHRSGETEDDFLA
                     DLAVATGAGQIKTGAPCRSERNAKYNELLRIEERLGEEAKYRNPFPQKGTGVREW"
     gene            4939..5175
                     /gene="secG"
                     /locus_tag="PLN77_06060"
     CDS             4939..5175
                     /gene="secG"
                     /locus_tag="PLN77_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRM43876.1"
                     /translation="MEILRTILTVIYVIDCIALTVVVLMQEGKQQGLGAIAGISDTYW
                     GRNKSRSAEGTLIRATKIMAVLFIVLSVVLNMNF"
     gene            5399..7228
                     /gene="rnr"
                     /locus_tag="PLN77_06065"
     CDS             5399..7228
                     /gene="rnr"
                     /locus_tag="PLN77_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRM43877.1"
                     /translation="MDLVSSKNYEPMRLKDIAMLLQVPKAKRSELAEVMEILVKEGKI
                     DVISDGRYQKSRGASTTTEKREKRDRRAGEQGRRGRDDRFRRKNGKNGDREKKPRIQT
                     VDIQAVVDAYQIPEEFPARVITQAEKCPEEVIPNDYNGRLDLKSWQMVTIDGEDAKDL
                     DDAVSVTKAGDTWTLGVHIADVSNYVQENSALDREALKRGTSVYLPDRVIPMLPKRLS
                     NGICSLNEGQERLALSCIMTINEKGRVIGHQIAETVIRVDRRMTYTAVNAILTEPEAH
                     PELLEEYHELVPMFRQMQELSALIRSCRKERGAIDFEFPESKVILDAEGTPVEIRPYP
                     SNVATRMIEDFMLMANETVAEEYCTREIPFLYRTHDKPDGDRMEATLTLIREQGIKVE
                     KRSHEITPGEVQKILTSIEGTPEEPLISRLLLRSMARAIYTPECSGHFGLAASYYCHF
                     TSPIRRYPDLQIHRIIKDDLRGRLDKGGRIGKYSSILQHVAEQSSAMEQRAAEAEREA
                     VKLKKAEYMLEHVGEEYDGVISGVTGWGFYVELQNTVEGLVHIMTLKDDYYNFDEENY
                     RLVGEQHGRVFRLGEPVRVRVRNAEPANRTIDFTYVEKETETE"
     gene            7248..7718
                     /gene="smpB"
                     /locus_tag="PLN77_06070"
     CDS             7248..7718
                     /gene="smpB"
                     /locus_tag="PLN77_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRM43878.1"
                     /translation="MAKQKGNRMIANNKKAYHDYFIEDKYEAGISLAGTEVKSLRMGK
                     CSIKESFIRIERDEVFIYGMHISPYEKGNIFNKDPLRIRKLLLHDYEIRKLEAQIKEK
                     GYTLVPLQVYFKGSLVKVEIGLARGKKLYDKRQDIAKKDQRREAERDFKVKNLY"
     gene            7847..8204
                     /gene="ssrA"
                     /locus_tag="PLN77_06075"
     tmRNA           7847..8204
                     /gene="ssrA"
                     /locus_tag="PLN77_06075"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2720 a         1959 c         2445 g         1953 t
ORIGIN      
        1 gtgtgtacaa agtgctgaaa agccagcagg aggaaatgct tccgaagctg ggagcacgtg
       61 tagagctgaa aaaaattctt gtgcgcaatg tggaaaaagc agctgcaaag gttgacgatc
      121 cctccatcat taccaccagc ttcgaggaga ttgccaatga cccggaaatc gagattgttg
      181 ttgaggttat gggtggaaaa cagccggcca tgaattacat tcttgcctgc ctggatgctg
      241 gaaagaatgt tgtaacggca aataaagatc tggtggcatc cgagggcaaa aaaatccttg
      301 caacagcaaa gagaaacgga aaagattttc tctatgaggc atccgtggcg ggaggaattc
      361 cgatcatccg tccggtgaaa cagtgccttg ccgccaatca tatcacagag ctgatgggta
      421 ttttcaacgg taccacaaat ttcattctca caaaaatgtc gcaggaaggc atggagtttg
      481 gcgatgcact ccgtctggcg caggacatgg gctatgcgga agcagacccg accgcagatg
      541 tggaaggtct tgatgccggc cgcaaggtag caatcctagc ttcggcagcg ttcaactcca
      601 gagtgacatt tgaagatgtt tacaccgaag gcattaccaa aatcacggcg accgatatcg
      661 agtacgcaaa agagatggga tgcacgatca agctgctcgg cgtggcaaaa gatacttcgg
      721 aaggaattga agcccgtgtt catccgatgc tgatcgacag caatcatccg cttgcgactg
      781 tcaatgattc ctacaatgcc gtattcctga caggcgatgc cgtacaggat acgatgtttt
      841 acgggcgagg tgccggagag cttccgactg cgagtgccgt tgtgggcgac attttcgatg
      901 ttgtgcgcaa tattttatgg aactgctgcg gcagaatcgg ctgcacatgc taccgggaat
      961 gcccgatcaa gcgtgtcggc gaaattaagt cgaaatattt tgtacgcatg cagattgaaa
     1021 atcggtatgg aacgctggca tcggtagctt ctgtctttgg aaacaacaag gtttcgattg
     1081 cacagatgct tcagaaacgt gtcaatggaa aatacgccga gatcgtcgtc attacggacg
     1141 aggtcaaaga gcgccatttt gaggatgcca tgcagatttt aggcggaatg tcgatgatcc
     1201 aggagatttc ttcgacaatc cgggtatact aacaaaatgt aacaattcag agccatgcga
     1261 aatggaagaa aaatcgcgaa tcatgcttgc atgaaggagc gctttttctg gagtttcata
     1321 tggcgaaaag caacatcgag atgcagcgta agcggaatcg agatggggct ctggattgtt
     1381 accccaaaat taaatcaaag aagggaattc acaatgagta aaaaaccaac cgtattaatg
     1441 attcttgacg gatatggact gaatgacaaa tgcgagcata acgctgtctg cgaagcaaaa
     1501 actccgatta tggaccagct gatgagccag tgcccgtacg taaaaggaca ggccagcgga
     1561 cttgctgtag gtcttccgga cggacagatg ggtaactctg aggtaggcca tctgaacatg
     1621 ggcgcaggcc gtgttgtata ccaggagctg accagaatca caaaagcaat ccaggacggt
     1681 gatttcttcg agaatgaaga gctggtaaag gctgttgaaa atgcaaaagc aaataattcc
     1741 gcactgcacc tgttcggact tctctctgac ggcggtgttc acagccacat cacccacgta
     1801 ttcggacttc tggagctggc aaaacgcaaa ggtctcgaga aagtttatgt acactgcttc
     1861 ctggatggcc gtgacactcc tccggcatca ggaaaatctt atgtagagca gctgcaggca
     1921 aaaatggatg agatcggtgt cggcgaaatc ggcgtaatct ccggccgtta ctatgcaatg
     1981 gaccgtgata accgctggga tcgtgtcgag ctggcttaca aagctctgac aaaaggggaa
     2041 ggcgtagaag gaaccgatgc agcagaggct gttcaggctt cctatgatgc tgaaaaaacc
     2101 gatgagttcg ttctgccgac cgttctgaaa aaagacggaa aaccggttgc aacgattcag
     2161 gataaagatt ctgtaatttt cttcaacttc cgcccggacc gtgcaagaga gctgacaaga
     2221 gcattctgtg cagatgagtt caccggattt gagagagaaa aacgtctgga tctgacctac
     2281 gtatgcttca cggaatacga tgagactatc ccgaacaaat ccgttgcatt caaaaaagaa
     2341 gagatcacca ataccttcgg tgagtatctg gcagcacata acatgacgca ggctcgtatc
     2401 gccgagaccg agaaatatgc acatgtaacc ttcttcttca atggcggcgt tgaggagcca
     2461 aataaaggtg aggacagaat ccttgtaaaa tccccgaagg ttgctactta tgacctgaaa
     2521 ccggagatga gtgcttacga agtatgcgac aagctgacgg atgctattcg ttcccagaaa
     2581 tatgatgtta tcatcatcaa ctttgcaaat ccggatatgg ttggccatac cggtgttgag
     2641 agcgcagcaa tccaggcagt tgaggctgtt gatgagtgcg taggcaaagc agttgatgca
     2701 ttaaaagagg ttgacggcca gatgttcatc tgtgctgacc acggaaatgc agagcagctt
     2761 gtagattacg agaccggcga gccatacacc gctcatacca ccaatccggt tccgttcatt
     2821 ctggttaatg cagatccatc ctacacgtta agagagggcg gatgcctggc agacatcgca
     2881 ccgaccctga tcgagctgat gggaatggag cagccaaaag aaatgacagg aaaatccctg
     2941 ctgattaaga aataaagaga aatcgtgaaa atgtccctgc cggattcgtt ttgaaacggg
     3001 ttcggcaggg atattttatt ataataatct gaaaataaaa ataattacgc aataagaaac
     3061 agaataaaaa tggaaaacag ataaaaaatc aaaataaagt gttgacaaac ggtgagaaaa
     3121 ctgatatagt aaagtcaaca aaagaaaacg gaagaaaaaa acagaaagag aggacggacc
     3181 accgagaaca taacgaatta ccccataaac taagaaagcg aaggtatgga cccaagaaaa
     3241 cgtaacgaag taccccaaag cgaaacaaaa gaacaaaaaa agaaaggatt cagtccaatg
     3301 cacagttcta acttatgaaa gccatccgaa aatggaaaaa tcggatggct ttcatattgc
     3361 gtttttttat gcttgaaaag aataaggact ggaaaaaaga ggtttcgaca gagagagcaa
     3421 catatttttc ccatagcacg gtcatactgt gaacgcagag aagaaacttg gttactgttc
     3481 actttgcagc aaaacaggag ggaaaactat gtttcgatat ttaccaattc gaagcgtgac
     3541 aggaagagaa attctggatt cgcgcggaaa tccgaccgta gaggtggaag tggccgtcgg
     3601 ggaggggatt ctcgggctgg aaggccatat ggggcgtgcc caggtgccgt ccggggcatc
     3661 gacaggaaaa tttgaggcac tggaaaaaag agatcaggaa gtgcgctatc tgggacaggg
     3721 cgtgagaaag gctgtagaag cggtcaatac cgtgctggcc gatgccgttg tcggcgagaa
     3781 tgcgctcgag caggagcgga tcgaccatat cctgtgccag gcagacggaa ccgaaacgaa
     3841 agaaaacctc ggggcaaacg ccattctcgg tgtctcactt gctgtggcgc gcgcggcggc
     3901 aaatgcgctt catctgccgc tgtaccagta cctgggcggc tgccacacaa gccgtatgcc
     3961 cgtgccgatg atgaatattt taaacggcgg ccgtcatgcc gacaacaccg tcgatctgca
     4021 ggaatttatg attatgccgt gcggcgctcc ctcattccgg gagggtcttt ccatgtgcgc
     4081 ggagatttac catacgctga agcagctcct gaaggagaag ggatactcga cttctgtcgg
     4141 cgatgagggc ggctttgcgc cggatttaaa ggattccgat gaagcactgc agctcattgt
     4201 caatgcggtc cagaaagccg gctatgcgcc cggaaacgac gtgatgatcg cgatcgatgc
     4261 ggcggcaagc gagctctacg aggagagcag gaacgtctac tactttcccg gggaatcccg
     4321 tcagtgcgga aagacagtat ttcgggatac cgcagaaatg gttgcctact atgaggaact
     4381 gatcgaaaaa tacccgatcg tatccatcga agacgggctg tgcgaggacg atttcgaagg
     4441 ctggaaacag atgacggaag cgctcgggaa cagagtacag ctggtcgggg atgatctgtt
     4501 tgtgacgaat gtaagaaggc tttcctgcgg cattgcactt ggcacggcaa atgcggttct
     4561 gatcaaggta aaccagatcg gaacgctctc tgaggcgctg gatgcggtgg agatggcgca
     4621 gagagcaggg taccgcgccg tcatttccca ccggtccgga gagacagagg atgattttct
     4681 ggcagacctt gccgtcgcga caggcgccgg ccagatcaaa accggagcac cgtgccggtc
     4741 ggagcggaat gcgaaataca atgagcttct ccgcatcgag gaacgcctcg gtgaggaagc
     4801 gaaataccgg aatccgttcc cgcaaaaggg aaccggcgta agagaatggt gaaaaacggc
     4861 gtaaaatagg caaataacag ttgaaatcgt tcttccgcta tggtaaaata atataagttg
     4921 gccgtaggag gtgtaattgt ggaaatttta agaacgattt tgacagtcat ttatgtcatt
     4981 gattgtattg ctttaaccgt tgttgtattg atgcaggaag gaaaacagca gggtctcggt
     5041 gctatcgcag gtatctcaga tacttactgg ggaagaaaca aaagccgttc tgcagaagga
     5101 actctgatcc gtgcaacaaa aatcatggca gttctgttta ttgtactgtc ggttgtatta
     5161 aacatgaatt tttagcaaag taaagacatc cttattccca cgagaaggcg taagggtgtc
     5221 tttttctaca aagaggagat aatagatgaa agataataag aagaaaaaag atcggaggaa
     5281 atatccgcag ggcagcgcgg cgtcccatgc aggacagaac agcgggaacc atggcggtca
     5341 tgcgggccat ccgcgttcca gaagttcgaa actgctgaaa aaccgcaaga cgctgctcat
     5401 ggatctcgta agctcgaaaa actatgagcc gatgcgactg aaagacattg ccatgctgct
     5461 gcaggtgccg aaagcaaaac ggtcggagct tgcggaggtt atggaaatcc tggtaaaaga
     5521 aggaaaaatc gatgtgatct ccgacggccg ttaccagaag agccgcggtg catccacaac
     5581 gacggaaaaa cgggaaaaaa gagaccgccg cgccggagag cagggcagaa gaggacgcga
     5641 tgaccgattc cgcagaaaga atggaaagaa cggcgacaga gagaagaagc cgcgtatcca
     5701 gactgtcgat atccaggcag tcgtggacgc ttaccagatc ccggaggagt tcccggcacg
     5761 cgtgatcaca caggcagaaa aatgcccgga ggaagtaatt ccaaacgatt acaacggccg
     5821 cctcgatcta aaaagctggc agatggtgac aatcgacggc gaggatgcca aagacctcga
     5881 tgacgccgta tccgtcacaa aagcgggcga tacgtggacg cttggcgttc atatcgctga
     5941 tgtcagcaac tatgtgcagg aaaattccgc cctcgaccgt gaggcgttaa agcgcggcac
     6001 cagtgtctat ctgccggacc gcgtcattcc gatgctgccg aagcgtctgt caaacggcat
     6061 ctgttccctg aatgaaggac aggagcgcct ggcactctcc tgtattatga cgatcaacga
     6121 gaaaggccgt gtgatcggcc atcagatcgc tgagacggtg atccgcgtgg accgccgcat
     6181 gacctacacc gccgtcaatg caatcctgac cgagccggaa gcacatccgg agctgctgga
     6241 ggaatatcat gagctggttc cgatgttccg ccagatgcag gaactgtctg cactgatccg
     6301 gagctgtcgg aaagaacgcg gagcaattga ttttgaattc ccggagagca aagtaattct
     6361 tgatgcagaa gggacgccgg tggagatccg cccgtatccg tcgaacgtgg caacacggat
     6421 gatcgaggac ttcatgctga tggcgaacga gaccgtggca gaggaatact gcacacgcga
     6481 aatcccgttc ttataccgga cgcatgacaa gccggacgga gaccgcatgg aggcaacgct
     6541 gacgctgatc cgtgaacagg gcatcaaagt cgaaaagcgt tcccatgaga ttacgccggg
     6601 tgaggtgcag aagattctca ccagcatcga gggaaccccg gaagagccgc tgatcagccg
     6661 cctgctgctc cggtcgatgg caagagccat ttataccccg gaatgcagcg gccacttcgg
     6721 actggcagcg tcatattact gccattttac ctcaccaatc cgccgttacc cggatctgca
     6781 gatccaccgc attatcaagg atgatctgcg gggacgtctc gacaagggcg gcagaatcgg
     6841 caaatattcg tccattttgc agcatgtggc ggagcagtcg agtgcgatgg agcagcgtgc
     6901 tgcagaggca gaaagagagg ctgtgaagct gaagaaagcc gaatatatgc tggagcatgt
     6961 cggcgaagag tacgacggcg tcatttccgg cgtgaccgga tggggcttct atgtggagct
     7021 gcaaaatacc gttgagggac tcgttcacat catgacactg aaagacgatt attataattt
     7081 tgatgaagaa aattaccgtc tggtcggaga gcagcacgga agagtattcc gccttggtga
     7141 gccggtgcgt gtccgtgtac gaaacgccga gcctgccaac cggacaatcg attttacgta
     7201 tgtagaaaaa gaaaccgaga cagaataaat ccctggagga aaaagctatg gcaaaacaaa
     7261 aaggaaatcg aatgattgcc aacaataaaa aggcatatca tgactatttt atcgaggata
     7321 aatacgaggc cggcatctcc cttgccggta ccgaggtaaa atccctccgc atgggaaaat
     7381 gcagcatcaa ggagtccttc atccgcattg agcgtgatga ggtattcatc tacggcatgc
     7441 acatcagtcc gtacgagaag ggcaacatat tcaataaaga cccgctgcgc atccggaaac
     7501 tgctgctgca cgactatgag atccgcaaac ttgaggcgca gatcaaggaa aaaggctaca
     7561 cgctcgttcc gctgcaggtg tattttaaag gcagccttgt aaaagtcgag atcggtctgg
     7621 cacgcggtaa aaaactgtat gataaacggc aggatatcgc aaagaaggat cagagacgtg
     7681 aggcggagag agatttcaaa gttaaaaatc tgtattaaaa atccttgaca aaaagtatgg
     7741 ggaaagacta taatacaata tacggcagtt gaaagagagt tttcactgca ccgggacgat
     7801 ctgtttgcgt ccccgtatca aagaaaagcg tctgtagaac cagcacgggc ttgtactggt
     7861 ttcgacgggg gttttgaagt ttgggaagcc attcgtggac tccgggaccg cgtcatcaac
     7921 ctggaaccta aatttaaacg cagacgataa ttacgtagcg tacgctgcct aagtgcggcg
     7981 ttgtcagctg aatagttttc cgcggctatt ctcctggcat caaacagatg cggagcactt
     8041 cgtttccaaa gctttgcgga gatggaagat tttatgaagc taccgaagcg gcaagcctgt
     8101 catccggcgt gccgatgggg gaacctttaa tagaatgact gtaatgggag atgcctgaat
     8161 gaataggctt tcggacaggg gttcgattcc cctcaggtcc atacctgaaa aaccgctaaa
     8221 atccagtgtt ttagcggttt ttttgtacat ttttgatgga aaaaacaggg caaaaatgcc
     8281 gctttagacc gtttcagacc gtttaaaacc gcatgaattt tggtcaaaaa agaaaggaaa
     8341 aattcgaaat tttgtcaaac tttatcttta ttcgcattgt taagaacgta aaagagaccg
     8401 tttcaaagtg ctgcaggcac atgaagtaga gatacttgat gtgcctgctt ttttgtacaa
     8461 taggaaagtt cgacttccgt cgaactcttg ataaaaagag cccgcacaaa ggcgggcatg
     8521 gcaaacagac gatagattaa aagaagtcaa tgtaaaagcc ttcttcaaat tcatcctcat
     8581 caagatcgtc atcttcggta agaaaatagt gcatatgcac tatttaaaaa cggtggctcc
     8641 ttataacaaa gatttctgtt cacaatataa aaaaatatcc agcaggttaa cttttgaagc
     8701 ggtaaaacaa atgcttaagg atgttttgag ttggtaaggg actaatagat tctgtacttt
     8761 tcgccctatg attgaatctg atcatgcgcg agaaaaggaa aataatagaa ttgacagact
     8821 tgaaaaaatg aattacgatt tttaaaagta ctgcattgct tgaatgagaa ttatatgtta
     8881 aggtaacaga tataaagaga actttaagca ttgatttatt aaaataaaca cggatagcga
     8941 ggtgtattta tgaccataga agaaattctt gcaggagaat ccaaaaacgt agaattcaaa
     9001 gagaacctgc cggaaaagag catcaaatat atgaagtctg tggttgcctt tgcaaatgga
     9061 accggagttt attggtg
//
LOCUS       DAOMUF010000082         8534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2122_length_8534_cov_3.730982, whole genome shotgun sequence.
ACCESSION   DAOMUF010000082 DAOMUF010000000
VERSION     DAOMUF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2122_length_8534_cov_3.730982"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(336..1607)
                     /gene="purD"
                     /locus_tag="PLN77_06080"
     CDS             complement(336..1607)
                     /gene="purD"
                     /locus_tag="PLN77_06080"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRM43879.1"
                     /translation="MKVLIVGSGGREHAIAWKVAQSPKVDKIYCAPGNAGIAEYAECV
                     DIAAMEFDKLVAFAKEKEIDLTVIGMDDPLVGGVVDAFEAEGLRVFGPRKNAAILEGS
                     KAFSKDLMKKYHIPTAAYETFSDPEAAEKYLETAKFPIVLKADGLALGKGVLICNTLE
                     EAKEGVKTIMLDKKFGSAGNEMVIEEFMTGREVSVLSFVDGKTIKTMSSAQDHKRAGD
                     GDTGLNTGGMGNFSPSPFYTEEVDKFCKKYIYQPTVDAMAAEGRPFKGVIFFGLMLTP
                     DGPKVLEYNARFGDPEAQVVLPRMKTDIIDVFEACIDGTLDQIDLQFADNACVCIVLA
                     SNGYPVSYEKGFPIRGLETFKEHEGYYCFHAGTKFKDGEIVTNGGRVLGVTALGDTLK
                     EARANAYKAVDWVDFDNKYYRHDIGKAIDEA"
     gene            complement(1613..2239)
                     /gene="purN"
                     /locus_tag="PLN77_06085"
     CDS             complement(1613..2239)
                     /gene="purN"
                     /locus_tag="PLN77_06085"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRM43880.1"
                     /translation="MLRLAVLVSGGGTNLQAILDAIDEGTITNAEVSVVISNNAGAYA
                     LERAKKKEIDAVCLSPKTFASREAFNEALLAKLQSYNVDLVVLAGCLVVIPQSIVDAY
                     PNKIINIHPSLIPSFCGTGYYGLKVHEGVLSRGVKVTGATVHFVDGGTDTGPIILQKA
                     VEVHEGDTPKALQQRVMEEAEWVIMPRAIDLIANGKVTVEDGHVKIKE"
     gene            complement(2233..3258)
                     /gene="purM"
                     /locus_tag="PLN77_06090"
     CDS             complement(2233..3258)
                     /gene="purM"
                     /locus_tag="PLN77_06090"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRM43881.1"
                     /translation="MDYKNAGVDIEAGYKSVELMKEHVKKTMRPEVLGGLGGFSGAFS
                     LAKIKEMEEPVLLSGTDGCGTKVKLAMILDKHDTIGIDAVAMCVNDIACAGGEPLFFL
                     DYIACGKNYPEKIASIVKGVAEGCLQSDAALIGGETAEHPGLMPEDEYDLAGFAVGVC
                     DKKDMITGENLAAGDVLIGMASTGVHSNGFSLVRKVFDMTKDSLNTYYDELGATLGET
                     LLAPTRIYVKALRAIKDAGVTVKACSHITGGGFYENIPRMLKDNTVAVVQKDSYTVPP
                     IFDLMAKKGNIDEQMMYNTFNMGIGMVLAVDPEDVDAAMKAIRSTGDTPYVIGHIENG
                     EKGVQLC"
     gene            complement(3284..3790)
                     /gene="purE"
                     /locus_tag="PLN77_06095"
     CDS             complement(3284..3790)
                     /gene="purE"
                     /locus_tag="PLN77_06095"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRM43882.1"
                     /translation="MAKVGIVMGSDSDMPVMSKAADMLEKFGIEYEMKIISAHREPDV
                     FFEYAKTAEEKGFKVIIAGAGMAAHLPGMCAAIFPMPVIGIPMHTTSLGGRDSLYSIV
                     QMPSGIPVATVAINGGANAGILAAKILATSDPELLAKLKAYTQEMKEQVEAKDAKLQE
                     TGYRNYSK"
     gene            complement(3913..5934)
                     /locus_tag="PLN77_06100"
     CDS             complement(3913..5934)
                     /locus_tag="PLN77_06100"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM43883.1"
                     /translation="MTDELLNERYALAIERIRQIPAEKSVPQPYRDFFAQMAQYLCKM
                     DQIRSRIAEGYLKTASEEELAVFNREPYEDVIGERYETSYGNPAFAVRALGETHGRSL
                     CCLYRELGNAVVWVYEDRLLELTAAMELYLELYAMFEEETLPSAQYVKESIYWYVSDY
                     AEERQEYQVREIVDPSLHFVKDIVMESDLTDLRYLYQYGEYITENEKGTARFLNTFSQ
                     EEIDAMARTYTEGFRKCFLVARKDLSKKKTVSIRFHIGFERMIRAAILQFREMGLEPV
                     ISRGARRTWVAGASANKQYDYDHRNDEALYLNEDLVKRRLRAMQVKYDEYKELAGGYA
                     GPAVVETFGEVPFEPVNKKQALHLNERQQKLRVGFQNEAGQIVNRYIKDDEYGYTIIA
                     YPMPEIDPRYEKIFREIVKINTLDYEKYQRIQQYLIDALDEGVSVHVLGKGENRTDLR
                     VMLHHLNDPAKETNFENCVADCNIPVGEVFTSPSLTGTTGVLHVTGVYLNELYYRDLC
                     LTLTDGMITAYDCANFEKEEDNRTYIEENLLYHHRTLPIGEFAIGTNTTAYVMAEQYG
                     IAGKLPILIAEKMGPHFAMGDTCYAWAEDSPMYNPDGKEVIARENEVSAKRKEDPSKA
                     YFGCHTDITIPYRELQSVAVEKADGTTIPLIEDGRFVLPGTEELNEPFG"
     gene            complement(6002..6613)
                     /locus_tag="PLN77_06105"
     CDS             complement(6002..6613)
                     /locus_tag="PLN77_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM43884.1"
                     /translation="MDRERFEKQLNFILEIDKEKQILRQTHIRGYSRQEDDAEHAWHM
                     AVMAFLLQEYSNEKIDIGRTMLMLLIHDLVEIDAGDTYAYDAEANKTKDEREHKAAER
                     IYGLLPEDQGKMLMDLWLEFEAYETPEARFAHVMDNFQPLLLNHENDGKDWTRHNVRI
                     SQPLKRNEKTATGSEEIWSYMEEILEQSIRDGKIIDDREEKQE"
     gene            complement(6680..6997)
                     /locus_tag="PLN77_06110"
     CDS             complement(6680..6997)
                     /locus_tag="PLN77_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43885.1"
                     /translation="MARKKYNYNFVRKPSEGGKEALYMAGASVFLFLLASVVSFAFRG
                     NGGIFLGAAGLFSFLLSGYGFYVGMKSFKEEQVSHRMSVLGSITSGGMLVAWLALFLI
                     GVR"
     gene            complement(6978..7454)
                     /locus_tag="PLN77_06115"
     CDS             complement(6978..7454)
                     /locus_tag="PLN77_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM43886.1"
                     /translation="MKQETKARIRVVGRILFLVYLLALVYFLFFAEEYGRRNFFELDY
                     RYNLVPFQEIRRFWIYREKVGFLAAFLNLAGNVIGFLPFGFIVPVMHKKMESFWKVSL
                     LGFMFSLCVETIQLITKVGCFDVDDLMLNTLGAMIGCGAFLICNKFRRWKYGEEEV"
     gene            complement(7539..7691)
                     /locus_tag="PLN77_06120"
     CDS             complement(7539..7691)
                     /locus_tag="PLN77_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43887.1"
                     /translation="MNEKIYKTMGRVGGGNIAMGAVLVVVGVATGVLMIVSGAGLLKR
                     RGEIMF"
     gene            complement(7726..8403)
                     /gene="pyrE"
                     /locus_tag="PLN77_06125"
     CDS             complement(7726..8403)
                     /gene="pyrE"
                     /locus_tag="PLN77_06125"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRM43888.1"
                     /translation="MEQYKQEFIEFMVESQVLKFGEFTLKSGRKSPFFMNAGAYVTGS
                     QLKRLGEYYAKAIHDNYGLDFDVLFGPAYKGIPLSVVTAIAIHDLYGVEVRYCSNRKE
                     AKDHGGDAGLLLGSKIKDGDRVVIIEDVTTSGKSIEETFPIIQAQGNVEIKGLMVSLN
                     RMERGKGDKSALEEIQETYGFPANAIVTMEDVITYLYNRPCQGKIQIDDKMKAAIDAY
                     YEEYGVK"
BASE COUNT         1842 a         2423 c         1869 g         2400 t
ORIGIN      
        1 tttttctttt tttttcaact ttttctcccg ccactctttc aggtactgct cctgttccag
       61 atcttcccgt tctttctccc atcctgccgc ccggcagagc gtccatgcaa gcagaagaaa
      121 tacggcaaaa atccccgcca gacaaacgat tcccatcttt tccgaccgcc tttcctgttc
      181 tgattctgct aaaaagcaaa ggatctcctt actattgtat ccggaacggc ggcgctttat
      241 tcccttttca gaataaaaag acaaagtcgc cagatttccc tttcaccggg attctggcgg
      301 ctccatttta ctcaaaatta aaattcacat tcacgttacg cttcgtcaat cgcttttcca
      361 atatcatggc ggtaatattt attgtcaaag tcaacccagt caacagcctt atatgcattc
      421 gcacgtgcct ctttcagggt atcgccaagt gctgtcacgc cgaggacacg gcctccgttt
      481 gtcacgatct cgccgtcttt gaacttcgtt cctgcatgga agcagtaata gccctcatgc
      541 tctttgaacg tctcgagtcc gcggatcggg aatccttttt cgtaggaaac cggataaccg
      601 ttcgaagcca gtacgatgca gacacacgca ttatctgcaa actgcaggtc gatctggtcg
      661 agagttccgt cgatgcaggc ttcaaataca tcaatgatgt ctgtcttcat acgaggaagc
      721 acaacctgtg cctccggatc gccgaagcgg gcattgtact cgagcacctt cggtccgtcc
      781 ggtgtcagca tcagtccgaa gaagataact cccttaaacg gtcttccctc ggctgccatc
      841 gcatctacgg tcggctgata gatatatttc ttacagaact tatccacttc ttcggtataa
      901 aacggactcg gggagaagtt gcccataccg ccggtgttca ggccggtgtc gccgtctcct
      961 gctctcttgt ggtcctgtgc ggaactcatc gtcttgatcg tctttccatc cacaaaggag
     1021 agaacggaaa cctcacgccc cgtcataaac tcctcgatga ccatctcgtt tccggcactg
     1081 ccgaatttct tgtcgagcat gatcgtcttg acgccctctt ttgcttcctc taaggtattg
     1141 cagatcagaa cgccttttcc aagagccaga ccgtcggctt tcagcacgat cgggaatttt
     1201 gcagtctcga gatatttctc tgccgcttct ggatcggaga atgtctcgta tgccgccgtc
     1261 gggatatggt attttttcat caggtctttg gagaacgctt tcgatccttc caggattgcc
     1321 gcattctttc ttggtccgaa tacgcgcagt ccctctgcct caaacgcatc gacaacaccg
     1381 ccgaccagcg gatcatccat gccgatgacg gtcaggtcaa tctctttttc tttcgcaaaa
     1441 gcaacgagct tgtcaaactc catcgctgca atatcgacac actctgcgta ctccgcaatt
     1501 cctgcgtttc caggagcgca gtaaatcttg tctaccttcg ggctctgtgc caccttccag
     1561 gcaatcgcat gctctcttcc gccgcttcct acaatcagta ctttcatcgc tcttactcct
     1621 ttatctttac gtgtccgtct tctaccgtta cttttccgtt tgcaatcaga tcaatcgcac
     1681 gcggcatgat gacccactcg gcctcttcca tgacgcgctg ctgcagtgcc ttcggtgtat
     1741 cgccctcgtg cacttcgact gccttctgca gaataatcgg gccggtgtct gttccgccgt
     1801 ccacaaaatg aaccgtcgct cccgtcactt tgacgccgcg ggataatacg ccctcgtgca
     1861 ctttcagtcc gtaatagccc gttccgcaga acgacggaat gagggacgga tgaatgttga
     1921 tgatcttatt cggataagca tccacaatgc tctgcgggat cacaaccagg catcctgcga
     1981 gcacaacgag atcgacgttg tacgactgca gtttcgccag cagtgcctcg ttgaatgctt
     2041 ctctcgatgc aaaggtcttc ggggaaaggc ataccgcatc aatctccttc tttttcgcac
     2101 gctccagcgc atacgctcct gcattgttgc tgatgacaac agaaacctcc gcgtttgtga
     2161 tggttccctc gtcaatcgca tccagaatcg cctgcaggtt cgtgccgccg ccggagacga
     2221 gaactgctaa tcttagcata actgtactcc tttttctccg ttttcaatgt ggccgatcac
     2281 atatggcgta tctcctgtgg aacggatggc tttcatcgct gcgtctacat cttccggatc
     2341 aacagccagt accataccga tacccatgtt gaaggtgttg tacatcatct gctcgtcgat
     2401 attgcctttc tttgccatca gatcaaagat cggcggtacg gtgtagctgt ctttctgtac
     2461 aacggctacg gtgttgtctt tgagcatacg cggaatgttc tcgtagaagc cgcctccggt
     2521 gatgtggctg catgctttta cggttacacc ggcatctttg atggcgcgga gtgcttttac
     2581 gtagatacgg gtcggtgcaa gcagggtctc gccgagggtt gcgccgagct catcatagta
     2641 tgtatttaaa gaatcttttg tcatatcgaa aactttgcgg acaagagaaa atccgttgct
     2701 gtgaacgccg gtggaagcca tgccgatcag tacgtctccg gctgccagat tttcaccggt
     2761 aatcatgtct ttcttgtcac atacgccgac agcaaaacct gccagatcgt actcatcctc
     2821 cggcatcagt cccggatgct ctgcggtctc accgccgatc agtgccgcat cggactgcag
     2881 acagccttct gcaacgcctt ttacgatcga agcgatcttc tccggatagt tctttccgca
     2941 tgcgatgtag tcgaggaaga acagcggttc tccgccggca catgcgatat cattcacaca
     3001 catggcaacg gcatcaatgc cgatggtgtc gtgtttatca agaatcatgg cgagcttcac
     3061 cttggttccg catccgtctg taccggaaag cagcaccggc tcttccattt ctttgatttt
     3121 tgccagggaa aaagcgccgg aaaaaccgcc aaggccgcct aatacctccg ggcgcatggt
     3181 tttctttaca tgctctttca ttaattctac tgatttgtat ccagcttcaa tatctactcc
     3241 tgcgttcttg taatccatgg tgaacctccc ttgtcctttg tttttattta ctgtagttac
     3301 ggtatcctgt ttcctgcagt tttgcgtctt ttgcctcaac ctgctctttc atttcctgtg
     3361 tatatgcttt cagttttgca agcagttccg gatcggaagt tgcaagaatc tttgctgcca
     3421 ggattccggc attggcaccg ccattgatgg caacggttgc taccggaatg ccgctcggca
     3481 tctgtacgat ggagtacagg gaatcacgac ctccaagcga tgtggtatgc atcggaattc
     3541 cgatgaccgg cattgggaaa atagctgcgc acatacccgg cagatgagct gccatacctg
     3601 cgcctgcaat gataaccttg aagcctttct cctctgcggt ttttgcatat tcgaaaaata
     3661 catccggctc gcggtgtgcg gagatgattt tcatctcgta ctcaattcca aatttttcca
     3721 gcatgtcggc tgctttgctc attaccggca tatcagagtc gctgcccatc acaattccta
     3781 ctttagccat gttgttctcc ttttctgatt aactgttcta agcgttattc attagttata
     3841 cttatattta tgttttaatc attcagttct gcttataata tactaagcag gcgcagaaat
     3901 gtcaacccat aatcagccaa aaggttcatt taattcttcc gttcccggca ggacgaagcg
     3961 tccgtcctca atgagaggga tcgtcgttcc gtctgctttt tcgaccgcaa ccgactgcag
     4021 ttcgcggtac ggaatcgtga tgtccgtgtg gcatccgaag tatgctttcg acggatcttc
     4081 tttccgtttt gccgaaacct cgttctcgcg cgcgattact tcttttccgt ccgggttgta
     4141 catcgggctg tcctccgccc aggcgtagca ggtgtcgccc atcgcaaaat gcgggcccat
     4201 cttctctgcg atcaggatcg gcagttttcc ggcgatgccg tactgctctg ccatcacgta
     4261 cgccgtcgtg ttcgttccga tggcaaactc accgatcggc agcgtcctgt ggtgatacag
     4321 caggttttct tctatataag tacgattgtc ctcttctttt tcgaagtttg cgcagtcata
     4381 cgcggtgatc atgccgtcgg tcagcgtcag gcagagatcg cggtaataca gctcgttcag
     4441 gtagactccc gtcacatgga gcacgcctgt cgtgccggta agactcgggg aagtaaagac
     4501 ctctccgacc ggaatattgc agtcggcaac acagttctcg aaattcgtct ctttcgcagg
     4561 atcgttcaga tgatgcagca tcacgcgcag atccgttctg ttctctcctt ttccaagcac
     4621 atgcacggaa acaccctcgt cgagcgcatc gatcaggtac tgctggatgc gctggtattt
     4681 ttcgtaatcg agcgtgttga tctttacgat ctcacggaaa attttttcgt agcgcggatc
     4741 gatctccggc atcgggtagg cgatgatcgt gtagccgtac tcgtcatcct tgatatagcg
     4801 gtttacaatc tggcccgcct cgttctggaa accgacacgg agcttttgct ggcgctcatt
     4861 taagtgcagt gcctgttttt tatttactgg ctcaaacgga acttcgccga aggtttccac
     4921 cacggcaggg ccggcatagc cgcccgccag ttctttgtac tcgtcgtatt tcacctgcat
     4981 cgcgcggagc cgtctcttca cgagatcctc gttgagatac agcgcctcat cgttccggtg
     5041 atcgtagtca tactgcttat tcgcagacgc accggcaacc cacgttctcc gcgcgccgcg
     5101 ggaaatcacc ggctcgagtc ccatctcgcg gaactgtaat attgccgcac ggatcatgcg
     5161 ctcaaaaccg atgtggaagc ggatgctgac tgtcttcttt ttcgacagat cttttcgcgc
     5221 cacgagaaag cacttgcgga agccttctgt gtaggtgcgt gccatcgcgt cgatttcttc
     5281 ctggctgaac gtatttaaaa agcgggctgt acccttctca ttttcggtga tatattcgcc
     5341 gtactggtac agataccgca gatccgtcag atcagattcc atcacaatat ccttcacaaa
     5401 gtgcagcgac gggtcgacga tttcgcgcac ctggtattcc tggcgctcct cggcgtaatc
     5461 gctcacatac cagtaaatgc tctcttttac atactgcgcg gacggcagcg tctcctcctc
     5521 gaacatcgca tacaactcga gatacagctc catcgccgca gtcagctcca gcaggcggtc
     5581 ctcatagacc cagacaacag catttcccag ctcccggtac aggcagcaga ggctccttcc
     5641 atgcgtttca ccaagtgccc gcacggcaaa cgccgggttt ccgtaggagg tctcataccg
     5701 ctccccgatc acatcttcat acggctcgcg gttaaaaaca gccagctctt cctcggatgc
     5761 tgtttttaga tatccttctg cgattctgct ccggatctgg tccattttgc agaggtactg
     5821 tgccatctgt gcaaaaaaat cgcggtacgg ctgcggaacg cttttttccg ccggaatctg
     5881 gcggatccgc tcaattgcaa gcgcataccg ctcgtttaat aattcatccg tcataaaaaa
     5941 atccctttct gtttctcttt aactgttacg aggggcagat ctgcccctca ttcttcttaa
     6001 cttattcctg tttttcttcc cggtcgtcaa taatttttcc atcccggata ctctgttcga
     6061 gaatctcttc catataactc cagatctcct cggagccggt cgccgtcttc tcgtttcgtt
     6121 ttaacggctg ggaaatccgc acattgtggc gggtccagtc ttttccatcg ttctcatgat
     6181 ttaaaagcag cggctggaaa ttgtccatca catgggcaaa gcgcgcttcc ggtgtctcat
     6241 acgcttcgaa ctcgagccag agatccatca gcatttttcc ctgatcctcc ggcagcaggc
     6301 cgtagatgcg ctcggccgct ttgtgttctc tctcgtcttt tgttttgttt gcctccgcgt
     6361 cgtacgcata cgtgtcgcct gcatcaatct ccacgaggtc gtggatcagc agcatgagca
     6421 tcgttctgcc gatgtcgatt ttctcattcg aatattcctg cagcagaaat gccatcaccg
     6481 ccatgtgcca tgcgtgctcc gcgtcgtcct cctggcgcga atagccgcgg atgtgcgtct
     6541 ggcgcaggat ctgtttttct ttatcaatct ccagaataaa attcagctgt ttttcaaaac
     6601 gttcgcgatc catctttttc cccttttctt ttatatgtac tcccggagtc tttctgtgct
     6661 tgattttacg agagtacatt tatctcacac cgatcagaaa cagtgccaac catgcgacca
     6721 gcatgccgcc gctcgtaatc gagccgagaa ccgacattct gtggctgacc tgttcttctt
     6781 taaagctctt cattcccaca tagaagccgt atcccgacag aagaaatgag aacagtcccg
     6841 ctgctccgag aaaaatgcct ccgtttccgc ggaatgcaaa cgatacgacc gaagccagca
     6901 gaaacagaaa gaccgaagca cctgccatat agagagcttc ctttccgcct tccgacggct
     6961 tccggacaaa attgtaatta tacttcttcc tcgccatatt tccatctcct gaatttattg
     7021 cagatcagga atgcgccgca gccgatcatg gctccaagcg tattcagcat cagatcatct
     7081 acatcaaagc agcccacctt cgtgatgagc tgaatcgtct ccacgcagag gctgaacata
     7141 aaaccaagaa gcgatacttt ccagaaactc tccatttttt tgtgcatcac cggaacgata
     7201 aagccgaacg gcagaaaacc gatcacattg cccgccagat ttaaaaatgc ggcaaggaat
     7261 cccacctttt cgcggtaaat ccagaaccgt ctgatttcct gaaacggcac cagattgtaa
     7321 cggtaatcca gttcaaaaaa gtttcttctc ccgtattctt ctgcaaaaaa cagaaaataa
     7381 acgagcgcaa gaagatacac cagaaataaa atccgtccca caacgcggat tctggctttc
     7441 gtctcctgct tcatcttcgt ttcccctttc tgcgtggtat cagaaacgcc ggaatacctc
     7501 ttcggcgcat gcgcttccaa aatattccgg catttttctt aaaacataat ttccccgcgt
     7561 cgtttcagca gacctgctcc gcttacgatc atcaggacac cggttgctac ccctaccaca
     7621 accagtactg cgcccatggc gatatttccg ccgccgacac gtcccatggt tttatagatt
     7681 ttttcattca tcgtgcaaaa ctcctttttt caagtgtacc acgtcttatt tgacaccgta
     7741 ctcctcataa taagcatcaa tggcagcctt catcttgtca tcgatctgga ttttgccctg
     7801 gcatggacgg ttgtacagat aggtgatgac atcctccatg gttacaatgg catttgccgg
     7861 gaagccatag gtttcctgga tttcctcaag tgcgctcttg tcgccttttc cgcgctccat
     7921 acggtttaag gataccatca gacctttgat ctccacattg ccctgtgcct ggatgatcgg
     7981 gaacgtctcc tcgatggatt ttccggaggt ggtaacatcc tcgatgatga caacacggtc
     8041 gccgtcttta attttgcttc caagcagcag tcctgcatct ccgccgtggt cttttgcttc
     8101 tttccggttg gagcagtaac gtacttccac accgtagaga tcatgaatcg caatagcggt
     8161 tacaacgctg agcgggattc ctttgtaggc cggtccaaac agaacgtcga aatccagtcc
     8221 ataattgtca tggattgctt ttgcataata ctcgccgaga cgttttaact ggcttccggt
     8281 tacgtaagcg cccgcattca tgaagaacgg ggattttctt ccgcttttca gagtgaactc
     8341 tccaaatttt aatacctggc tctcgaccat aaactcgata aactcctgct tgtactgttc
     8401 catctttttt cctccatatt ttcaggggaa ggcatctttt ttcgcaatgc cgcccgtttt
     8461 tacttctatt cttttctata ctaccataaa cgcctctgtt tttccagtcc agacacagga
     8521 tttcgcataa aaaa
//
LOCUS       DAOMUF010000083         8446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2154_length_8446_cov_2.785246, whole genome shotgun sequence.
ACCESSION   DAOMUF010000083 DAOMUF010000000
VERSION     DAOMUF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2154_length_8446_cov_2.785246"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3910
                     /locus_tag="PLN77_06130"
     CDS             <1..3910
                     /locus_tag="PLN77_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding Tp47 domain C-containing
                     protein"
                     /protein_id="HRM43889.1"
                     /translation="LTKYKEVKDTDKVDITVTNRGQTSTQTYTGKDALFENSDYAYYV
                     LSEAPSYYKEATVNADGSLSFGKTVGTAIELTNVSPEFMTESKYGDYQLDLDGLENTI
                     DVGTDQIYGVIVSTKEGNDYGMRHVENIWRGTELAWSTGFTTQVHGCLTSSAHYKSMM
                     GQHINKVTYYTSKGIYTIPVNDIFVPTKFEASVSVEDASLSAGKTTMTVTGLPADYDA
                     EYSVDGLDGFAVDGNTISYSANAKKGMYTLTIHDKKGVYADLTQSFTLYTDAIPAAYN
                     EKGSAPALVKAENASEDDFADYIKNITSVKVNGKEYAATGKRKIILIKEDGTLDLTAA
                     AFAEGNKFEIEVTATGYKTLSFTYEKKETTYKYVYAGLTWAEYWAAEGVQAAGSDAGS
                     DETDSREESDKGAFDVVTRATTNHGLHRGSFQCAATIYMKDGTTYDVAYYTAATTAVL
                     TNGETVTYDKSQIDHYEVKGLKYVPVKVSEDDYEDFCKKYVVVENGGTLSGGFSENKL
                     QSYTGLVADVTENTNGLKIAVKNADGSFSFSARTTGSESGIKDQALKTAPDAETVGLT
                     VKEANGSYGEFLRVDLTGDYGDLAANLQTVTWTYYGDDSTYTNAKATYGTKFAADNWM
                     HKVMGIQLGLTDSLRCTLPEGTDGTGYWKITIHALGYADASYTFEATKANIVGADPVE
                     STAALEEAIAKADALNKDLYTETSWNNMQTELAEAKEALEKKQSQGTVDEATEHLNAA
                     IEALQSQYVLMNIPYAAFYQAEVHNDVPVDAFTSATKNKTRTASLSGGSYHVNADGSD
                     ITGITFPVKVTEDMDLSKYTKVTDEDSVEITVTNRGQTSTTTYTGKDALYENASYAYY
                     TLHEVPSYYKEASVDADGNVTFGAVQGTEAAKLENVTAELSTNSKYGDYQLDLDGLTD
                     TIKAGEDTVYGVVVSTKEGNDYGMRHLENIWRVSELAWSTGFTTSVHNCPTSSEHYKS
                     MMGQTINKITYYTSKGIYEVDADVYVPVKFAYEFSVENASVTSGNASVKLEGLPDDYD
                     AVYTVEGLTGTVSGDTLYFKNAEKGSYTLTVSDKNGKYADLSADFILYTEEMPAAFNE
                     DTTTPGLLKNGDATDEEFADYLKNITSVTVNGKAYAASGRGAVVLFNEDGSLKTDAAP
                     FAEGDSFEITVSSLGYQDLSFTWKKSAEEPTPTPTETPTVTPTETPTVTPTETPTVTP
                     TETPTVTPTTAPTTTPTTAPTTIPTTQPTKTPTSTPKATSTPLASNKVSNTSTSKKSD
                     NAKTGDATNIWGWVSMAVASLGAGGLGFKLRGRKKNEDEE"
     gene            4108..4644
                     /locus_tag="PLN77_06135"
     CDS             4108..4644
                     /locus_tag="PLN77_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3877 family protein"
                     /protein_id="HRM43890.1"
                     /translation="MTAKLLEKHITDTIREWQVKIGYEGGTMKLYYPAESLRRSLSLD
                     ETEDLDAALAAFCKEVQPRLGTLAISAVKDRYCMEIPEEGCSYIERKIPVPEFLQNLL
                     QVITTPGNTMEQVRNCFSSYAEKMHTTVEENASEEHEMGHVFSFSDPSVDEYCYCVEE
                     NEFGLTYHRFSREDYEAL"
     gene            4665..5201
                     /locus_tag="PLN77_06140"
     CDS             4665..5201
                     /locus_tag="PLN77_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRM43891.1"
                     /translation="MEFTKLLEERRSIRAFDPEKHVTAEQIQEIVQAAIQAPSWKNSQ
                     TTRYYALVTPEKVEEFSAKCLPEFNQKSSKGAALVVTAFVKDRSGFTQDGTPDNEVGN
                     GWGYYDLGLHDENFILRARELGLDTLIMGIRDEKAIREALSIPENELLGAVIAVGYRA
                     INPDKPKRKTVDDILKLF"
     gene            complement(5187..6056)
                     /locus_tag="PLN77_06145"
     CDS             complement(5187..6056)
                     /locus_tag="PLN77_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM43892.1"
                     /translation="MILEYLLAKIEKSDVIYRCIGNEMKNGILACGFMRKETADRSQY
                     HFSNEYYSCFVLLRGSGEYIAEDGTSYPLQAGSLVQRLPGVPHSTRVDPDGKWLEFFI
                     SIGKPFFDSFCSLSVLNREPVLKAELLPGDLERYQKLLQSLKATPDSLLPLRIPEMEK
                     EILRMYGYHAHRVNLQRDPIEAACDMLSQNLDEEISLEELARSLQIGYETFRKVFKKQ
                     TGVSPARYRTRKKMEQARILLEGGVPMKEIAGLVGYGDVYAFSKQFSRFFGFPPGKYR
                     ARLHTDAPDLEQF"
     gene            6282..>8446
                     /locus_tag="PLN77_06150"
     CDS             6282..>8446
                     /locus_tag="PLN77_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRM43893.1"
                     /translation="MRQTRDWENQYITQKNRYPMHTPYGAYETVEQALAGNRNASRFV
                     MDLNGDWKFKMYPSPEAVEAFYEENFDHAAWDTIPVPSNWELQGYGKPVYTNMLYPFK
                     REANGEAFEIEITEGTYELNAPYVPEKNLTGCYFRTFEVPTDYIGRQLLIDFGGVESC
                     FYLWVNGKQVGYSQDSKVNAEFDITEYVQEGTNTLAVQVMRYCDGTYLEDQDYWHLSG
                     IYRDVRLVSKPVQHILDYKAETLFTHPDYTKATLSVTIWPDNSKPLYGEYHAKVTLYD
                     AEQHKVTEMASRPFAECGFYLMPKFIATVTAPVENPALWTAETPTLYTLVVELQNKEN
                     ETVDIESCKVGFRQVEINSRGVLTLNGKRLVIRGVDRHDFCAETGRTVSEEQMRKEIA
                     VMKRLNFNAVRTSHYPNSVKWYDLCDELGIYLVDEANLETHGYGGQLSASAEWTAAYL
                     ERATRMVLRDKNHPSIVLWSLGNESGAGANHAAMHGWIREYDKTRYVQYESGNPKSNI
                     SDVICPMYPTMSWLEDVMADDSDLRPFIMCEYAYAKSNSNGNFKEFWDYVNKYPRFQG
                     GFIWDFADKAIAIHEEDGNIKYGYGGAFGEDVTDPVPDMCLNGVVFADLSYKPGAYEV
                     RNGQSPVTIEQVKNPYTGETIWVLANRYHTLDLSHLQVRYEIVQNGETLAKGSYPVFY
                     TAAGTTETLPLPELPEQLHGEAYVNYYVELAQDTFYAPTG"
BASE COUNT         2527 a         1919 c         2073 g         1927 t
ORIGIN      
        1 tcttacaaaa tacaaagaag taaaagatac ggataaagtt gatattacag taacaaaccg
       61 tggtcagaca tctacacaga catacaccgg aaaagacgcc ttatttgaaa actcagacta
      121 tgcatattat gtattaagcg aagcaccttc ttactacaaa gaagcaacgg taaatgcaga
      181 tggctctctt tcttttggaa aaacagttgg aactgcaatt gaactgacca atgtatctcc
      241 ggaatttatg actgaatcaa aatatggaga ttatcagctg gatctcgacg gactggagaa
      301 cacgattgat gttggaaccg atcagatcta tggtgttatt gtcagcacga aagaaggcaa
      361 tgactatggt atgcgtcatg tggaaaatat ctggcgcgga acagagctgg catggtcaac
      421 tggatttaca acacaggtac atggatgcct gacttcttct gcacattaca agagcatgat
      481 gggacagcat atcaataagg ttacttatta taccagcaaa ggaatctaca cgattccagt
      541 gaatgatatt tttgttccaa caaaatttga ggcaagtgta tccgtagaag acgcttctct
      601 ttccgctgga aagacaacga tgaccgtaac cggacttccg gctgattatg atgcagaata
      661 ttccgtagat ggtctggatg gatttgcagt agacggaaat accatttctt attccgcaaa
      721 tgcgaaaaaa ggaatgtata ctctgacgat tcatgataaa aaaggtgttt atgcagatct
      781 gacacagagc tttactctgt atacagatgc aataccggct gcttacaacg aaaaaggaag
      841 tgctccggcg cttgtaaaag cagagaatgc atcagaagat gactttgctg attatatcaa
      901 gaacatcact tcggtaaaag taaatggaaa agaatatgca gcaactggaa aacggaaaat
      961 aattctgatc aaagaagacg gtacgcttga tctgacagct gccgcttttg cagaaggaaa
     1021 taaatttgaa atcgaagtaa ctgcaacagg ttacaagacg ctttccttta cttatgagaa
     1081 gaaagaaaca acatataaat atgtatatgc aggtcttacc tgggctgagt attgggcagc
     1141 agaaggagtt caggcggcag gaagcgacgc tggttcggat gaaactgaca gtcgcgaaga
     1201 aagtgataaa ggcgcttttg atgttgtaac ccgtgcgact acaaaccatg gactgcacag
     1261 aggaagcttc cagtgcgcag cgactatcta catgaaagat ggcacaacct atgatgtagc
     1321 atattatacc gcagcaacca cggcagttct gaccaacggc gagaccgtaa cttacgacaa
     1381 aagccagatt gatcattatg aagtaaaagg cctgaaatat gtgcctgtaa aagtttcgga
     1441 agatgattac gaagatttct gcaaaaagta tgttgtggta gaaaacggcg gaacactttc
     1501 cggtggattt tctgaaaata aactgcagag ctatacaggc ctggttgcag atgtaacaga
     1561 aaacacaaac ggtctgaaga tagcagtgaa aaatgcagat ggaagcttct ccttctctgc
     1621 aagaacaaca ggaagtgaat ccggaatcaa agatcaggcg ctgaagacag ctccggatgc
     1681 tgagaccgta ggactgaccg tgaaagaagc caatggttct tatggagaat tccttcgcgt
     1741 agatttaacg ggagattacg gcgatctggc tgctaatctg cagaccgtaa cctggactta
     1801 ttacggtgat gattccactt atacgaatgc aaaagcaacg tatggaacaa aattcgcagc
     1861 agataactgg atgcataaag taatgggaat ccagcttggc cttaccgatt ccctgcgctg
     1921 cactttaccg gagggaacgg atggaaccgg ttattggaag attacgattc atgcacttgg
     1981 ctatgcagat gcttcctaca cttttgaagc aaccaaagcg aatattgtag gagctgatcc
     2041 ggtagagagc acagcagcac tggaagaggc aattgcaaag gcagatgcac tgaataaaga
     2101 tctttacacg gaaacttcct ggaataatat gcagacagaa ctggcagaag caaaagaagc
     2161 attggagaaa aaacagagcc agggaaccgt agacgaagca acagagcatc tgaatgcagc
     2221 catcgaagct ctgcagtccc agtatgtatt aatgaatatt ccgtatgcag cgttctatca
     2281 ggcagaggtt cataatgatg ttccggtaga tgcgtttact tccgcaacaa agaataaaac
     2341 aagaaccgcg tctctttccg gcggatctta tcatgtgaat gcagacggaa gcgatattac
     2401 aggtatcacc ttcccggtaa aagtaacaga ggatatggat ctgagcaaat acacgaaagt
     2461 gacagatgag gattctgtag aaatcaccgt taccaaccgt ggacagacat ccaccacaac
     2521 gtataccgga aaagatgcac tgtatgagaa tgcaagctat gcatattaca ctttacatga
     2581 ggttccgtct tattacaaag aagcatctgt agatgcagac ggcaatgtaa cctttggtgc
     2641 tgttcaggga accgaggcag caaagctgga aaatgtaacg gcagagctct ccactaacag
     2701 caaatacggt gattatcagc tggatctgga tggtcttact gataccatca aagcaggcga
     2761 agataccgta tatggtgttg ttgtcagcac aaaagagggc aacgattacg gaatgcgtca
     2821 tctggagaat atctggagag tgtctgagct ggcatggagc actggattta cgacaagtgt
     2881 tcataactgc ccgacttctt ctgaacatta caagagtatg atgggacaga caatcaacaa
     2941 gattacgtac tacaccagca agggaattta tgaagttgat gcagatgtat atgtaccggt
     3001 taagtttgca tatgaatttt ctgtagagaa tgcgtctgtc acatctggaa atgcttctgt
     3061 taaactggaa ggcctgccgg atgattacga tgctgtctat acagtagaag gtctgacggg
     3121 aaccgtgtcc ggtgatactc tgtacttcaa gaatgcagag aaaggttctt atacattgac
     3181 tgtcagcgat aagaatggta aatatgcaga tctttccgca gactttatcc tttacacgga
     3241 agagatgccg gcagcattta acgaagatac gacaacaccg ggtctgctta agaacgggga
     3301 tgcaacagat gaagagtttg cagattatct gaagaacatt acctctgtta ctgtaaatgg
     3361 aaaagcatat gcagcaagcg gaagaggagc tgttgtactg ttcaatgagg atggttcctt
     3421 aaagacagat gcagcaccgt tcgcagaggg cgattccttt gaaattacag tttcttcact
     3481 gggatatcag gatcttagct tcacctggaa gaaatcagca gaagagccga ctcctactcc
     3541 tacagagacc ccaaccgtaa ccccgaccga gactccgact gttaccccga cggaaacccc
     3601 gaccgtaacc ccaactgaga ctccgacggt tacacctaca acagcaccaa ccacaacccc
     3661 gacgactgct cctaccacaa tacctacgac gcagccgacg aagaccccga caagcacccc
     3721 gaaagcaaca tctactccgc ttgcaagtaa taaagtatcc aatacttcta cttccaagaa
     3781 gagcgataat gcaaagaccg gtgatgctac caacatctgg ggatgggtaa gcatggcagt
     3841 tgcatctctt ggcgctggcg gacttggatt caaactccgc ggcagaaaga aaaacgaaga
     3901 cgaagagtaa ttcagaacaa gatgactgaa aaattcttct gatcataaga acccacagcc
     3961 ccttccgatc cagattttct ggcggagtgg gctgtgggtt tctttacata aaaacagaaa
     4021 attttttctt gaaacagaag acaattcgtg cgatgataat actgtgcttt tttgcacatg
     4081 cagcacaatg gaggtgactt agaaaacatg acagcaaaac tgttggaaaa acatattacc
     4141 gatacaattc gggaatggca ggtgaagatc ggctatgagg gcggtacgat gaagctctac
     4201 tacccggcgg agtccctgcg ccgttccctc tctttagacg aaacagaaga cctggatgcg
     4261 gcacttgccg cgttctgtaa ggaagtacag ccgcgtcttg gaacacttgc gatttcagcg
     4321 gtcaaggacc gctactgcat ggaaatcccg gaggagggat gttcttatat cgagcggaaa
     4381 attcctgttc cggagttcct gcagaacctt ctgcaggtga tcacaacgcc tggaaacaca
     4441 atggaacagg ttcgaaactg cttttcttcc tatgcagaga aaatgcatac taccgtggaa
     4501 gaaaacgcat ccgaggagca tgagatgggc catgtatttt ctttttcaga cccgtctgta
     4561 gatgaatact gttactgtgt cgaagaaaat gaattcggcc tgacgtatca ccgtttttca
     4621 cgggaagatt acgaggcttt ataaataaaa ggaggatata caccatggaa tttacaaaac
     4681 tgctggaaga aagaagaagc atccgcgcgt ttgatccgga gaaacacgta accgcagagc
     4741 agatccagga gatcgtacag gcagcgattc aggcaccatc ctggaaaaac tcacagacaa
     4801 cacggtacta tgcccttgta acaccggaga aagtcgaaga attttccgca aaatgccttc
     4861 cagaattcaa tcagaagagt tcaaagggag ccgcacttgt cgtaacagca ttcgtaaaag
     4921 accgctccgg cttcacgcag gacggaacac cggacaatga agtcggaaac ggctggggct
     4981 attacgatct tggcctgcat gacgaaaact tcattctgcg cgcaagagaa cttggccttg
     5041 atactctgat tatgggaatc cgtgatgaga aagctatccg tgaagccctc tccattccgg
     5101 agaatgagct tcttggtgct gtcattgctg tcggctaccg tgccatcaac ccggacaagc
     5161 cgaaacgtaa aaccgtggac gatatcttaa aactgttcta aatccggcgc atccgtgtga
     5221 agacgtgcgc ggtactttcc aggcgggaat ccaaagaacc gggaaaactg cttgctgaac
     5281 gcatagacat cgccatatcc gacaagaccg gcaatttcct tcatcggtac gccgccctcg
     5341 agaagaatac gggcctgctc cattttcttc ctggtgcggt accgtgcagg agagacgccg
     5401 gtctgttttt taaatacttt tcgaaacgtc tcataaccga tctggagtga ccgggcaagc
     5461 tcttcgagag aaatttcttc atcaagattc tgcgagagca tatcgcaggc ggcctcgatc
     5521 ggatcccgct gcagattcac ccggtgcgca tggtagccgt acattcgcag aatctctttt
     5581 tccatctccg gaatccgcaa aggcagcaga ctgtccggtg tggcctttaa cgactgcaga
     5641 agcttctgat accgctcgag atcgccggga agcagctctg cctttaacac cggctcccgg
     5701 ttcagaaccg aaagcgagca gaaagaatca aaaaacggtt tcccaatcga gataaaaaat
     5761 tccagccatt tcccatcggg gtctacgcgt gtggaatgcg gcacaccagg cagacgctgg
     5821 accagacttc ccgcctgcag cggataggag gtgccgtcct cggcaatata ttccccgctc
     5881 ccgcgcagca gcacgaaaca gctgtaatac tcgttcgaaa aatgatactg cgagcggtcc
     5941 gccgtctctt tccgcataaa accgcacgcc agaatcccgt tcttcatttc atttccaata
     6001 cagcgataaa tgacatcgct cttttctatt tttgcaagca gatattccag tatcatacga
     6061 cttctccttt gctttcccat tttggataaa agaaacacca gatcaactat ttctcatcca
     6121 cgtgcagtgt tttatactga atcataagat aactgcataa atcttctgaa aatcacagat
     6181 gtattatatc acaggaaagc aaaaaaggaa caccaatctg gataaaaaga agagaggcgg
     6241 tcggaaaatg cgaaagcaaa ggcaaggagg atttcaagaa catgagacag acaagagact
     6301 gggaaaatca gtatataacg cagaaaaatc gttatccgat gcatacgccg tacggtgcgt
     6361 atgagacggt agagcaggcg ctcgccggaa atcggaacgc gtcccgcttt gtaatggatc
     6421 tgaacggaga ctggaaattc aaaatgtatc cgtcaccgga agcggtggaa gcattttatg
     6481 aagagaattt cgatcacgca gcatgggata cgattccggt gccgtccaac tgggaactgc
     6541 agggatatgg aaagccggtt tacaccaata tgctgtatcc gttcaaacgg gaggcaaacg
     6601 gcgaagcatt tgagatcgag attaccgaag gcacctacga gctgaatgcg ccgtacgtac
     6661 cggagaaaaa tctgactggc tgctatttca gaacctttga ggttccaaca gactatatcg
     6721 gccgacagct tctcatcgat ttcggcggtg tggagtcctg cttctatctc tgggtcaacg
     6781 gaaaacaggt ggggtattcc caggacagca aagtgaacgc ggaatttgac atcaccgaat
     6841 atgtgcagga aggaacgaac accctcgccg tacaggtgat gcgctactgt gacggtactt
     6901 acctggaaga tcaggattac tggcatctgt ccggtatcta ccgcgatgtc cgcctcgtat
     6961 cgaaaccggt gcagcacatc ctggactaca aagcagagac gctgttcaca cacccggatt
     7021 acacgaaagc tactctctct gtcacgatct ggccggacaa ctccaaaccg ttgtacggcg
     7081 agtatcatgc caaagtgacg ctgtatgatg cagaacagca caaagtaacc gagatggctt
     7141 cgagaccgtt tgctgagtgc ggcttctatc tgatgccgaa atttatcgca accgttactg
     7201 ctccggtaga gaacccggca ctctggactg cagagacccc gacactttac accctcgttg
     7261 tcgagctgca gaacaaagag aatgagacgg ttgatatcga gagctgcaag gtcggcttcc
     7321 gtcaggtgga aatcaacagc cgcggtgttc tgaccctgaa tggcaaacgc cttgtgatcc
     7381 gaggtgttga ccgccacgat ttttgcgccg agacgggacg taccgtttct gaggagcaga
     7441 tgcgaaaaga aattgctgtg atgaaacgcc tgaatttcaa cgctgttcgt accagccatt
     7501 atccgaattc cgtgaaatgg tatgatctat gtgacgagct cggcatttac cttgtcgacg
     7561 aggcaaatct tgagacgcac ggttacggcg gacagctcag tgcatccgct gagtggacag
     7621 ccgcttatct ggagcgtgca acccgtatgg tactgcgtga taaaaaccat ccttccatcg
     7681 tcttgtggtc ccttggaaat gaatccggtg ccggggcgaa tcatgccgcg atgcacggct
     7741 ggatccgcga atacgacaag acaagatacg ttcagtatga gtccggcaac ccaaagagta
     7801 atatttccga cgttatctgc ccaatgtatc cgacgatgag ctggcttgag gatgtgatgg
     7861 cagacgattc cgatctccgc ccgttcatca tgtgcgaata tgcgtatgca aagagcaaca
     7921 gcaacggaaa cttcaaggag ttctgggact atgtcaataa atacccgcgt ttccagggcg
     7981 gctttatctg ggatttcgcg gacaaggcga ttgcgatcca cgaggaggac ggaaatatca
     8041 aatacggcta cggcggagcg ttcggagagg atgtgaccga tccggttccg gatatgtgtc
     8101 tgaatggtgt tgtctttgcc gatctttcct ataaaccggg cgcctatgag gtgagaaacg
     8161 gccagtcccc ggttacgatt gagcaggtga aaaatccata taccggcgaa actatatggg
     8221 tgctggcaaa ccgctaccat accctcgatc tttctcatct gcaggtgcgt tatgaaattg
     8281 tgcagaacgg cgaaacgctt gccaaaggca gctatccggt attttacacg gcagccggaa
     8341 caacagagac gcttccgctt ccggagcttc cggaacaact gcatggcgag gcgtatgtaa
     8401 attattacgt tgagcttgcc caagatacct tctatgcacc gacggg
//
LOCUS       DAOMUF010000084         8444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2156_length_8444_cov_3.878055, whole genome shotgun sequence.
ACCESSION   DAOMUF010000084 DAOMUF010000000
VERSION     DAOMUF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2156_length_8444_cov_3.878055"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..953)
                     /locus_tag="PLN77_06155"
     CDS             complement(18..953)
                     /locus_tag="PLN77_06155"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRM43894.1"
                     /translation="MRYLTKTLDFDSRGDSVVTLGKFDGLHRGHMLLIRRVLAIGRTE
                     GLETVVFTFDVPPQSKVQHVKPHQLLTNEERRARLEQLGMDCLVECPFVDEVMHMTPE
                     AFIKEILVDKLHAKKVVVGKDFGFGYKRSGTAEMLKEMGPSLGFETEIIEKAEENGRE
                     ISSTWAREELEQGNMEAVSGLLGYDYAVHGEIVHGHALGRTIGVPTINQIPPAEKLLP
                     PFGVYVSEVKIEGKIYYGITNIGVKPTVQEKFTGVETNLFDCSEDLYGKQAEVSLLKF
                     LRPEQKFASIDALKNQLDHDVAAGKKYVEKKFAQQ"
     gene            complement(950..1876)
                     /gene="truB"
                     /locus_tag="PLN77_06160"
     CDS             complement(950..1876)
                     /gene="truB"
                     /locus_tag="PLN77_06160"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRM43895.1"
                     /translation="MINGVLNVWKEAGFTSHDVVAKLRGILHQKKIGHTGTLDPDATG
                     VLPVCLGKGTRLCDMLTDETKTYEALLHLGIATDTQDMSGTVTAEAPVTVTEEEVRDC
                     IASFVGEQQQVPPMYSALKVNGKKLYELAREGIEIERKARTVHFYEISILEVKLPLVR
                     MEVTCSKGTYIRTLCHDIGEKLGCHGCMEQLIRTRVGQFDRNGAHTLDEIAAIVGEGR
                     LSEILVPVDEMFAALRSFATADDHAKKLAENGNPLKPAQIREIGETASGGDFAEDEEF
                     RLYLDDGTFVGIYQYRPSQNLTRPVKLFLERT"
     gene            complement(1949..2932)
                     /locus_tag="PLN77_06165"
     CDS             complement(1949..2932)
                     /locus_tag="PLN77_06165"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRM43896.1"
                     /translation="MKKIDEILNGVSCMAIAGHVRPDGDCIGSCLGLYQYLRDNRPEI
                     QADVYLEDPRPEFSYLPGFSEVKTSCEEKAYDLVVLLDVSSEERIGVAAPLLAKAGKT
                     ICIDHHVTNTEYCEINHVEPDASSACEVLFGLLEEEKISEPCAEALYTGIVTDSGVFQ
                     YSSTSPHTMRVAAALMEKGVPFTRIIEDAFFKKTYVQNQIMGRTLTESIMLLDKQCIV
                     GIVRKKVMDFYGLTPGDMDGIVSQLKNTEGVHVAIFLYELEPQVFKVSLRSDELVDVS
                     KIAAVFGGGGHVCAAGCTMSGSPYDVINNLTLYIEKQLLAEEEEETTEEEA"
     gene            complement(2919..3305)
                     /gene="rbfA"
                     /locus_tag="PLN77_06170"
     CDS             complement(2919..3305)
                     /gene="rbfA"
                     /locus_tag="PLN77_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRM43897.1"
                     /translation="MRKNSIKNTRINGEVQKELSNIIRNEIKDPRIGMMTSVTAAEVA
                     PDLKTCKVYISVFGDDEAKKETIRGLKSAEGFIRRMLAKTINLRNTPELTFVLDESIE
                     YGVTMSKLIDEVTHKEDKKDEENEEN"
     gene            complement(3321..5930)
                     /gene="infB"
                     /locus_tag="PLN77_06175"
     CDS             complement(3321..5930)
                     /gene="infB"
                     /locus_tag="PLN77_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRM43898.1"
                     /translation="MEMKKPEEQAKPKKKKIISVVRPQNATSKEAKEFTKNQGQKKDR
                     PSRGNGRPEGNRNGNGRSEDRPNRGNGNGGNGDRPNRNGDRPQGERRFNNNGDRPNRN
                     GDRPQGERRFNNNGDRPNRSGDRPQGERRFGGNNGDRPNRSGDRPGGDRNNGGRSGNG
                     ERSGRPFGERSARPNGGRPGNNGGRPFAGKDDDSQNQNRRPGGDRRRTEHKSSAGDTM
                     SLGIKKPGRDTGRKEKDKNKDSLRENKFSEDRGRGGNRPNQGHHQQSRLPKALQKPVN
                     KPKQEAPKDEVKEITLPEKMTIRELAEKMKMQPAVIVKKLFMEGTMVTVNHEIDFEKA
                     QEIALDYDIIAEPEEKVDVIEELLKEDEEDEKDMVPRPPVVCVMGHVDHGKTSLLDCI
                     RNTHVTDREAGGITQHIGAYTVSINKQKITFLDTPGHEAFTAMRMRGANATDIAILVV
                     AADDGVMPQTVEAINHAKAAGVEIIVAINKIDKPGANVERVKQELSEYELIPEDWGGS
                     TVFVPVSAKNNEGIDDLLEMILLTSEVLELKANPNRKARGLVIEAELDKGKGPVATIL
                     VQKGTLHVGDYIAAGACSGKVRAMMDDKGRRVKEAGPSTPVEILGLSDVPNAGEILVQ
                     TDNDKEAKNFAATFVAENKKNLLEETKAKMSLDDLFNQIQEGNLKELALVVKADVQGS
                     VEAVKQSLVKLSNEEVAVRVIHGGVGAINESDVMLATASSAIIIGFNVRPDTNAKSMA
                     EQNGVDIRLYRVIYQAIEDVEAAMKGMLEPVYEEKVIGHAEVRQLFKASGVGTIAGSY
                     VLDGTFQRGCKVRISREGNQIFEGNLASLKRFKDDVKEVRSGYECGLVFEGFNDLQEF
                     DQVEAYIMVEVPR"
     gene            complement(6026..6337)
                     /locus_tag="PLN77_06180"
     CDS             complement(6026..6337)
                     /locus_tag="PLN77_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRM43899.1"
                     /translation="MNSQSKGLSMLGLAMKAGKVSSGEFATEQAVKSGSAYLVILAET
                     ASANTQKKFRNMCAYYKVPCILFGDKEQLGRAIGREYRSSLAVCEAHFAEKIAALLAE
                     A"
     gene            complement(6321..6599)
                     /locus_tag="PLN77_06185"
     CDS             complement(6321..6599)
                     /locus_tag="PLN77_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRM43900.1"
                     /translation="MSTVRKVPLRKCIGCNEMKNKKEMIRVVKTTEGTIVPDTTGRMN
                     GRGAYVCSLECLGKAMKNKGLSRSLKAEIPSEVYEQLKEAFAKIEQSK"
     gene            complement(6630..7814)
                     /gene="nusA"
                     /locus_tag="PLN77_06190"
     CDS             complement(6630..7814)
                     /gene="nusA"
                     /locus_tag="PLN77_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRM43901.1"
                     /translation="MNTELMEALEVLEKEKNISKDTLLEAIEQSLIQACKNHFGKADN
                     VKVSINAQTGDFKVVAEKTVVDLVQDPIMEIALSDAKRADSRYELGDIVPVEIKSKEF
                     GRIATQNAKNVILQKIREEERKVLFDQYFEKEHDVVTGVVQRYVGRNVSINLGKVDAL
                     LTENEQVKGEKFEPTQRVKVYVLEVKNTTKGPKVMVSRTHPELVKRLFESEVSEVAEG
                     IVEIKSIAREAGSRTKMAVWSNDPNVDAVGACVGMNGSRVNAVVNELNGEKIDIITWD
                     ENPALLIENALSPAKVISVMADPDEKTAKVIVPDYQLSLAIGKEGQNARLAARLTGFK
                     IDIKSETQAREAGDFDYVEEEEYEEEGNYDESAAEEETISAEEDFDENDVETAEKAGD
                     EE"
     gene            complement(7846..8313)
                     /gene="rimP"
                     /locus_tag="PLN77_06195"
     CDS             complement(7846..8313)
                     /gene="rimP"
                     /locus_tag="PLN77_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRM43902.1"
                     /translation="MSRREEYEQKAEELITPIVEANGFELVDVEYVKEGGTWYLRAYI
                     DKEGGITVDDCEIVSRAFSDRLDAEDFIDDAYIMEVSSPGLGRPLKKEKDYKRSMGKE
                     LEIRTYRAIERNREFYGILTAYDDNSVTILEEDGTERVFQKSDIALIRLAFDF"
BASE COUNT         1619 a         2410 c         1898 g         2517 t
ORIGIN      
        1 gcgaaacagc aagtgtttca ctgctgagcg aacttttttt ctacatattt ttttcctgcc
       61 gcaacatcat gatcgagctg gtttttcagc gcgtcgatcg aagcaaattt ctgctccggg
      121 cgcaggaatt ttaacagaga aacctccgcc tgttttccat agagatcctc gctgcagtca
      181 aaaagattcg tctccacgcc ggtaaatttt tcctgtaccg tcggcttaac accgatgttt
      241 gtgataccgt aataaatttt tccttcgatt ttcacctcgg aaacgtagac gccgaacggc
      301 ggaagcagct tttctgccgg cgggatctga ttgatggtcg gcacgccgat cgttctgccg
      361 agtgcatgac cgtggacaat ctcgccgtga accgcgtaat cgtagccgag cagaccgctc
      421 acggcttcca tattgccctg ttccagctct tcccgtgccc aggtgctgct gatctcgcgg
      481 ccgttttcct ctgctttttc gatgatctcg gtttcaaaac caagggacgg gcccatttct
      541 ttcagcattt cggctgtgcc gcttctcttg tagccaaaac cgaaatcctt tcccacaaca
      601 acttttttcg cgtgcagctt atccaccaga atctctttga taaaggcttc cggcgtcatg
      661 tgcatcactt catccacaaa cgggcattcc acaaggcagt ccatgccaag ctgttcgagg
      721 cgggctcttc tctcctcatt cgtcagcagc tgatgcggct tgacatgctg cactttcgac
      781 tgcggcggca cgtcaaacgt aaacacaacc gtttccagtc cttccgttct gccgattgca
      841 aggacacggc ggatcagaag catatgcccc cggtggaggc cgtcaaattt tcccagggtt
      901 acaacgctgt cccctctgct gtcaaaatcc agtgtttttg ttagatatct catgttctct
      961 ccagaaataa cttgaccggt ctggttaagt tttgtgatgg tctgtattga taaataccga
     1021 caaacgttcc gtcatcgaga taaaggcgga actcctcgtc ttctgcgaag tccccgccgg
     1081 aagcagtttc cccgatttcg cggatctgcg ccggtttcag cggatttccg ttttccgcaa
     1141 gctttttcgc atgatcgtcc gctgtcgcaa agctgcgcag cgcggcaaac atttcatcga
     1201 ccggcacgag gatctccgaa aggcggccct caccgacaat cgctgcgatc tcgtcaagcg
     1261 tgtgcgctcc gtttcggtca aactggccga ctctcgtgcg gatcagctgc tccatacatc
     1321 cgtggcagcc cagtttttcg ccgatatcgt ggcacagggt gcggatatac gttcccttcg
     1381 aacacgttac ctccatgcgc accagcggaa gcttcacttc cagaatgctg atctcgtaaa
     1441 agtgcaccgt gcgggccttc cgctcgatct cgatgccttc ccgcgccagc tcgtagagtt
     1501 tctttccatt aaccttcagc gcggaataca tcggcggcac ctgctgctgc tccccgacaa
     1561 acgaggcgat gcagtcccgc acctcttctt ccgtcacggt caccggcgct tctgctgtca
     1621 cggttccgct catgtcctgc gtatccgttg cgattccaag gtgaagcagc gcttcatacg
     1681 tcttcgtttc atcggtcagc atatcgcaga gacgcgttcc ctttccaagg cagaccggaa
     1741 gcacgcccgt cgcatccggg tctagggttc cggtgtggcc gatttttttc tggtgcagga
     1801 tgccgcgcag cttcgcaacc acgtcatgcg acgtaaatcc cgcctctttc catacgttca
     1861 gaactccgtt tatcatgttg ttttgtctcc ctctttttca caagaaaacc gggcgtggat
     1921 atattttctg cgcccggctg tttcctgttt atgcttcttc ctctgtcgtc tcttcttcct
     1981 cttctgcaag aagctgtttt tcgatataca gagtcagatt gttgattaca tcatacggag
     2041 aacctgacat cgtgcagcca gccgcacaga catggccgcc gccgccgaat acggctgcga
     2101 ttttgctcac atcaaccagc tcgtcggagc ggaggcttac tttgaagacc tgcggctcca
     2161 gctcatacag gaagatcgct acgtgaacgc cttctgtatt ttttagctgg ctgacaatgc
     2221 cgtccatatc tcccggtgtc aggccataga agtccatgac cttcttccgc acgataccga
     2281 caatacactg cttgtcgaga agcatgatgc tctctgtcag cgtgcgtccc ataatctggt
     2341 tctgtacgta tgttttcttg aagaatgcat cctcgatgat tctggtaaac ggaactccct
     2401 tttccatcag agcggccgca acccgcatcg tgtgcggaga ggtgctgctg tactggaata
     2461 cgccggaatc ggtcacaata ccggtgtaca gcgcctcggc gcacggctcg gagatctttt
     2521 cttcctccag aagcccgaac agcacctcgc aggcagagct tgcatccggc tctacgtgat
     2581 tgatttcgca atattctgta ttcgtcacat ggtgatcgat acagatcgtc tttcccgctt
     2641 ttgcaagaag cggtgcggca acgccgatgc gctcctcgct ggatacatcc agaaggacca
     2701 caaggtcgta cgctttctct tcacagcttg tctttacctc agaaaagccc ggaagatagg
     2761 aaaattccgg ccgtggatcc tcaagataga catctgcctg aatctccggc cgattgtcgc
     2821 gaagatactg gtataatccc aggcaggaac cgatgcagtc accgtccgga cgaacgtgtc
     2881 cggcgatggc catgcaggag actccgttta gaatttcatc aattttcttc attttcctca
     2941 tcctttttgt cttctttgtg tgttacttca tcaatcagtt tcgacatcgt aacgccatat
     3001 tcaatcgact catccaggac aaaggtcagc tccggtgtat tgcggaggtt aatggtcttg
     3061 gccagcatac ggcggatgaa gccctccgcg ctctttaagc cgcggatggt ttctttcttc
     3121 gcctcatcat cgccaaacac actgatatat actttacacg tcttcagatc cggcgctacc
     3181 tctgccgctg tgacagaggt catcattccg atgcgcggat ccttgatctc gttccggatg
     3241 atgttggaaa gctctttctg tacttccccg ttgatcctgg tgtttttaat actgtttttt
     3301 ctcaacttaa ttcctcattt ctatctcggc acttctacca tgatataggc ctcgacctga
     3361 tcgaactcct gaagatcgtt gaatccttca aatacaagac cgcactcata gccggagcgc
     3421 acttctttga catcgtcttt gaaacgtttg agggatgcca gatttccttc gaagatctgg
     3481 tttccttcgc ggctgattcg aactttgcag cctctctgga acgttccgtc cagtacatag
     3541 cttccggcga tcgttccgac accggatgcc ttgaagagct gacgtacctc ggcatggccg
     3601 ataactttct cttcgtaaac cggctcaagc atacccttca tggctgcctc aacatcctca
     3661 attgcctggt agatgacacg gtacagacgg atgtctacgc cgttctgctc tgccatggat
     3721 tttgcgttcg tatccgggcg tacgttaaat ccgataatga tggcggaaga tgcggttgcc
     3781 agcataacgt cggactcgtt gatcgcaccg acaccgccgt ggatgacgcg tacggcaact
     3841 tcctcgttgg agagtttgac aagactctgt tttactgcct cgacagatcc ctgtacatcg
     3901 gctttgacaa cgagtgccag ttcttttaag ttaccctcct gaatctggtt gaacagatca
     3961 tccagggaca tcttcgcttt tgtctcctca agcaggttct tcttgttctc tgccacgaag
     4021 gttgctgcga agttctttgc ttctttatcg ttatcggtct gaacgaggat ctctcctgcg
     4081 ttcggaacat cggaaagtcc gaggatctca accggagtag acgggcctgc ctctttgacg
     4141 cggcgtccct tatcatccat catcgcacgg actttaccgg agcatgcgcc tgctgcgatg
     4201 taatcgccga catgcagggt acctttctgt accagaatgg tagcaaccgg tccttttcct
     4261 ttatccagct cagcctcgat gacaagacct cttgctttcc ggttcgggtt tgctttcagc
     4321 tcgagcactt cggaagtcag aaggatcatc tcaagcagat cgtcgatacc ttcgttgttc
     4381 tttgcagata ccggaacgaa tacggtgctt ccgccccagt cctccggaat cagttcgtac
     4441 tcggagagct cctgttttac acgctctacg tttgcgcctg gtttatcgat cttattgatc
     4501 gcaacgatga tctcaacgcc ggctgctttt gcatggttga ttgcctcaac ggtctgcggc
     4561 atcacaccgt cgtctgctgc gacaacgaga atggcgatat ccgttgcatt ggctccacgc
     4621 atacgcatag cggtgaatgc ctcatgtcct ggggtatcga ggaacgtaat cttctgtttg
     4681 ttgatcgaaa cggtgtatgc accgatgtgc tgggtaatac cgccagcctc gcggtcggtg
     4741 acatgggtgt tccggatgca gtccagaaga gatgttttac cgtggtcaac gtgtcccata
     4801 acgcagacta ccggcggacg cggaaccatg tctttttcat cctcttcgtc ctcttttaag
     4861 agctcttcga tcacatcgac tttctcttcc ggttctgcga tgatatcgta gtccagtgcg
     4921 atctcctgag ctttctcaaa atcgatctca tggtttacgg taaccatcgt tccttccatg
     4981 aacagtttct ttacgatgac tgccggctgc attttcatct tctcagccag ctcgcggatg
     5041 gtcattttct ccggcagggt aatctccttt acctcatcct tcggagcttc ctgtttcggt
     5101 ttgttgaccg gtttctggag cgctttcggg agtctggact gctgatgatg tccctggttc
     5161 ggacggtttc cgcctcttcc tctgtcttca ctgaatttat tttctcttaa agaatctttg
     5221 tttttgtctt tttcttttct tcctgtatca cggcctggct tcttgatgcc aaggctcatt
     5281 gtatcgcctg cagagctctt atgctcggtt ctgcggcggt ctcctcccgg acggcggttc
     5341 tgattctggg aatcatcatc ctttcctgcg aacggtcttc cgccattatt tcccggtctg
     5401 cctccgtttg gacgtgcgga acgctcaccg aatggtcttc cgcttctctc accgtttccg
     5461 cttcttccgc cgttattgcg gtctcctccc ggacggtctc cgctgcggtt cgggcggtct
     5521 ccattgtttc cgccgaagcg gcgttctccc tgcggacggt ctccgctgcg gttcgggcgg
     5581 tctccgttat tgttgaagcg gcgctctccc tgcggacggt ctccattgcg gttcggacgg
     5641 tctccgttat tgttgaaacg gcgctctccc tgcggacggt ctccattgcg gttcggacga
     5701 tctccatttc cgccgtttcc gtttccacgg ttcggacggt cctcgcttct tccgtttcca
     5761 ttgcggtttc cttccggacg gccgtttccg cgggacggac ggtctttttt ctgtccctgg
     5821 ttctttgtga actcttttgc ttctttactt gttgcgttct gcggacgcac aacggaaata
     5881 attttctttt tcttcggttt tgcctgctct tccggtttct tcatttccat attttctgta
     5941 tctttctttt ccaatgatct tttctcctct ttctgcggtt ttcgtgtttc ctgcatccat
     6001 ccaccgacct ttccggcctt acgccttacg cttctgccaa caatgctgct attttttctg
     6061 caaaatgcgc ttcacatacg gcaagggaag aacggtattc tcttccgatg gctcttccaa
     6121 gctgctcttt gtccccaaaa agaatgcatg gcactttata gtatgcacac atattgcgaa
     6181 atttcttctg tgtattggcc gacgccgttt ccgccagaat gacaaggtat gcactgccgc
     6241 tctttacggc ctgctcggtc gcaaattcgc cgctcgatac cttacccgcc ttcatggcaa
     6301 ggcccagcat ggataatcct ttactttgac tgttcaatct tcgcaaatgc ctcctttaac
     6361 tgctcataaa cctctgatgg aatttctgcc ttcagagaac gggaaagtcc tttgtttttc
     6421 attgctttcc ccagacattc cagagagcag acataagcac cgcgtccgtt cattcttccg
     6481 gttgtgtccg gaacaatcgt tccttcggtg gttttcacca cgcggatcat ttcttttttg
     6541 tttttcattt cattgcagcc aatgcattta cgcagcggca ctttacgaac tgtactcaaa
     6601 tgctcatccc gcctttgttt ttattttaat tactcttcgt ctccggcttt ctctgctgtt
     6661 tctacatcat tctcgtcgaa gtcttcttct gcagagatgg tttcctcctc tgctgcggac
     6721 tcatcgtaat tgccctcttc ctcatattcc tcttcttcta cgtagtcgaa gtctcctgcc
     6781 tcgcgtgcct gggtctcgct cttgatgtcg atcttgaacc cggtcagacg tgcggcaagt
     6841 cttgcgttct gtccctcttt accgatggca agggaaagct ggtaatccgg aacgatcact
     6901 tttgcggtct tctcatccgg gtctgccata acggagataa cttttgccgg gctcagtgcg
     6961 ttctcgatca ggagtgccgg gttctcatcc catgtgatga tgtcgatctt ctcgccgttc
     7021 agctcgttga caaccgcgtt gacacggctt ccgttcatac cgacgcatgc gccgactgca
     7081 tcgacattcg gatcgttgga ccatactgcc atttttgtac ggcttcccgc ctcacgggca
     7141 atgctcttga tctctacgat gccttccgca acctcggaaa cctcagactc aaagagacgt
     7201 tttacgagtt ccggatgggt acgggatacc ataaccttcg gtcccttggt tgtattcttt
     7261 acttccagaa cgtatacttt cacacgctgg gtcggctcaa atttctcgcc tttgacctgc
     7321 tcgttctcgg tcagcagagc gtctacttta ccgaggttga tgctgacgtt gcgtcctaca
     7381 tagcgctgta ccacaccggt aaccacatcg tgctctttct cgaagtactg atcaaacaga
     7441 actttacgct cttcctcacg gatcttctga agaatgacgt ttttggcatt ctgggttgca
     7501 atacggccga actctttgga tttgatttct accggaacga tatcgccgag ttcatagcgg
     7561 gagtctgcac gttttgcatc ggaaagggcg atctccatga tcggatcctg taccagatca
     7621 acaacggttt tctctgctac gactttgaaa tcaccagtct gggcgttgat gcttaccttt
     7681 acattatctg ctttgccaaa atggttttta caggcctgga tcagcgactg ctcaattgcc
     7741 tcaagcaggg tatctttgct gatattcttc tctttctcaa gaacttcaag ggcctccatt
     7801 aattctgtat tcatttttct cttttcctcc tgtatctgcg tctgtttaga aatcaaatgc
     7861 taaacggatc aatgcaatat cgcttttctg gaagacgcgc tctgttccgt cctcctccaa
     7921 aatggtcaca ctgttatcat catatgcggt gagtattccg taaaattccc ggtttctctc
     7981 gatcgcacgg tatgtccgga tttccagctc ttttcccatg cttctcttgt agtctttctc
     8041 ttttttcagc ggacgtccaa ggcccgggga gctgacctcc atgatatagg cgtcatcgat
     8101 aaagtcctct gcgtccagcc ggtcagaaaa tgcacggctg acgatttcgc agtcatctac
     8161 ggtaattccg ccttctttgt cgatgtaagc acgcagatac caggtaccgc cctctttgac
     8221 atactccaca tccaccagtt caaaaccatt ggcttctaca atcggagtga tcagttcttc
     8281 tgccttctgt tcgtattctt ctcttctgct caaatcctca ccaccttttt gattcaggga
     8341 aactttcgaa aaattctttc atttccctgt acacaagaaa gagtggacga aatcgtccac
     8401 tccttaacag tcacttatta acagctttac tatagcacct tttc
//
LOCUS       DAOMUF010000085         8373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2195_length_8373_cov_3.406107, whole genome shotgun sequence.
ACCESSION   DAOMUF010000085 DAOMUF010000000
VERSION     DAOMUF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2195_length_8373_cov_3.406107"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLN77_06200"
     CDS             <1..253
                     /locus_tag="PLN77_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM43903.1"
                     /translation="KSRKDAENMISLPLKFDEVESVEEFVNMVKRLDYDVDVKIGRCV
                     FDAKSLMSVLMIAGNPDAVVEAHTNDIEAFKKKFKDYLQ"
     gene            440..3775
                     /locus_tag="PLN77_06205"
     CDS             440..3775
                     /locus_tag="PLN77_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM43904.1"
                     /translation="MEEEKLERSIRKSEIPGLTREEEEAQLAKVIGIAEQNLEQAKAD
                     IRSANEDLADLMETYDAKEAEGLALWNNATAKLNAYQHGMARLEKARKKPYFGRIDFQ
                     DPRLSFLESYYIGRVGISDEKAEPVVLDWRAPISSVYYENSTGPCSYTVSSEGTFSID
                     LKRKRTYEIENDHLKDFFDSDVVANDELLTKYLAKNKKAVLGEIIATIQQEQNLIIRR
                     SPKTNLIVQGVAGSGKTTVAMHRISYILYNYEEDFRPEDFYIIGSNRILLNYITSVLP
                     ELDVYGIRQMTMEQLFIRLLYEDWDEEKYMVHAIDRADEKNSIKGGSGWFFDLENFCR
                     TYEAEQIPREPVRLEKTGTLLLDAEYIDNYCREQSTLSMEGKMCMLNEILLAKFENEV
                     SGKEVTFPAREKKALKRKYEKYFGDGKWRGSIFDLYLQFLEQQAEKGKAVEVPENSFD
                     VYDLAALAYLYKRIKENDPVREASHVVIDEAQDFGMMAYQVLHYCLRDCTYTIMGDTS
                     QNIHFSYGLNDWEELKKLILTGTYDAFGVLRKSYRNTVEISDFANEILRHGDFAIYPV
                     EPVLRHGTAVRKEAFDDEAALLAAGVQTIKTWQAQGYETIAVVCRDEAEAADTARKLK
                     QYVPVVEEDLETAEFGEGVMVLPVAYTKGLEFDAVLLLDPTEEKYPENDGQVKLLYVA
                     ATRALHELAVLYTGELTGILAKEAPKGRHNQEFAMETLTKAKEYEKVSLTQKEAREEN
                     RAIGIQEMDERNSHGPKRIVIKPEQRPGMALGNTSTTGTAVMAKGSTATRRAAAETGE
                     EIAAKTMQQRAPEGVSSIFAGAAYHGKAGGKAPGSRRSAGTQAGMQYGTGTAGVQSAK
                     RPGGAGMVPGEAEKTERLNTSPYAFGAIPENELLRIKGHSKIKCAVKWAKKGKSAVEI
                     ASMYGILRITPITPEVIRISFVKGVTAKVQDTYWKPKADTAFPWSAKESKTAVLVETE
                     KLRVMVEKKDGAVQFLTPDNKPILSEKRDEPRMIDGGMTWTFFDWSGSEKLKAKGILS
                     TEWLDLTAKARYVSFGGKQARMPLLVSNRGYGIAAAASRTALLCNVRTFGTYLHTAGD
                     GQIDYYFILGKDREEIVKQYKEL"
     gene            3792..5981
                     /locus_tag="PLN77_06210"
     CDS             3792..5981
                     /locus_tag="PLN77_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HRM43905.1"
                     /translation="MKTLVIAEKPSVARDIARVLGCSKKENGALEGNKYIVTWALGHL
                     VTLKDPEDYDKKYKEWNMAVLPMMPEKLQIKVIPQTGKQYHAVTTQLARKDVNEIVIA
                     TDAGREGELVARWILDKAHNQKPCKRLWISSVTDKAIREGFANLKDAKEYDNLYRAAV
                     SRAEADWLVGINATRALTCKYNAQLSCGRVQTPTLAMIAQREQEIREFVPKPYYTVTA
                     AAGGVVYTWKDEKSGGTRISDPEKAKQIAEKAKRYPLVLQNVEKKKKQKEAPLLYDLT
                     ELQRDASARFGYSAKQTLNLMQSLYETHKVLTYPRTDSRYLTKDIVPTLKERLDAVSI
                     GPYKALAQQAKRSPLNGKLFFVNDAKVSDHHAIIPTEQYVQLSALSNEERRIYDLVVR
                     RFLAVLLPPCVYEETVIQASIEKECFTARGKRMVEKGWQEAYEDNRYDEEDEAKEEVR
                     EQNLPLLEAGMKIGQPKISLREGKTKPPARFTEGTLLSAMENPVRYMENRDSSLVKTI
                     GEAGGLGTVATRADIIEKLFRSFLLEKKGNEIYLTSKARQLLKLVPADLKKPELTASW
                     EMQLNDIAKGKKRRDVFMKEIRSYTVELIDEIKTEEGTFRHDNLTNKKCPNCGKRLLA
                     VNGKNAKLLVCQDRECGYRETVSRTTNARCPVCHKRMEMIGKGEDATFVCACGHKERM
                     TKFQERRKKEGGGVTKRDVAAYMKKQKKEAEEPVNNAFAAALKGIKL"
     gene            6158..>8373
                     /gene="bglX"
                     /locus_tag="PLN77_06215"
     CDS             6158..>8373
                     /gene="bglX"
                     /locus_tag="PLN77_06215"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HRM43906.1"
                     /translation="MKQTDLEKLLADMSLKEKVDQMLQLSGAFYLGDENSVLTGPAND
                     MGIGKEDLEMAGSILGAAGADTMKKLQDDYMAKQPHHIPMLFMMDVIHGMKTIFPAPL
                     AQGATFDPELSGECASAAAKEASAAGLHVTFSPMVDLVRDARWGRVVESTGEDPYLNS
                     RFSEAIVKGFQGDDLKTPGKVASCIKHFAGYGGAVAGRDYNTVELSEHTFREFYLPAY
                     KAGIDAGAAMVMTSFNTINGVPASTNKWLMRDILRGEMGFDGVLISDFAAILETVAHR
                     SSKDAADAAKKALEAGVDIDMMTSVYAANLCRLVEEGEVDEHLIDECCLRILELKNKL
                     GLFENPYKDADAEKEKAYNLCPEHRALAKKAAEESFVLLKNDGVLPLDTAKKIAFVGP
                     YTNNHEIKSSWSFTGDSKDCVTIQEAAEKVFDASRTTYAEGCPVIGNDVELIGFTETT
                     PKKYSEEELAAMEQSALQAAKEADLVVMPMGEHYLQSGEATSRAMIDVPEIQMELFRK
                     VLAVNPNVVVVLFNGRPLDLREISAKAKAILEVWFPGTEGGSAVVDVLTGKKNPSGKL
                     PMSFPYCVGQAPVSYNEYSTGRPNLPGMKERFRSKYLDIPNAPLYPFGYGLSYTTFAV
                     SDVVLDQNEMDKNGRIAAKVTVKNTGDAAGCEVVQLYIRDDYSDVVRPVKELKGFRKV
                     ELAPGEEQEVTFYITEEDLRYYRLDGTFGSEPGTFTVMIGNSSTTENSAVFTLTPV"
BASE COUNT         2323 a         1879 c         2557 g         1614 t
ORIGIN      
        1 aaaaagcaga aaggatgcag aaaatatgat ttcgttaccg ttaaagtttg atgaagtaga
       61 gagtgtagaa gaatttgtaa atatggtgaa acgcctggat tacgacgttg acgtaaaaat
      121 aggacgatgc gtctttgatg cgaaatccct gatgagtgtc ctgatgatcg ccggaaaccc
      181 ggatgcggtt gtggaagccc atacgaacga tatcgaagca tttaaaaaga aatttaagga
      241 ttaccttcag taagaaaaaa agtcggaaag gcactgtgtg taagatgcat ggtgtctttt
      301 ttgatgcacg aaaaagcggg tgaagtatct tgccggggag acgtgtgcat ggtaaaataa
      361 agtctattgg gatggaaata agagaatcaa aagaacgggc tgatgtagaa gaagtttttc
      421 gtaaaaaagg aggcacaaag tggaagaaga gaaactggaa agaagcatca gaaaatcaga
      481 gattcccgga cttacgaggg aagaggagga agcacagctt gcgaaggtga tcgggattgc
      541 cgagcagaat ctggaacagg cgaaggcgga tatccggtcg gcgaatgagg atctggcgga
      601 tctgatggag acgtacgacg caaaagaggc ggaaggcctg gcgctctgga acaacgcgac
      661 ggcgaagctg aatgcgtacc agcacggcat ggcgcggctg gaaaaagcga gaaagaagcc
      721 gtatttcggg cggattgatt ttcaggatcc gcggctttcg tttttagagt cctattacat
      781 cgggcgcgtc ggcatttccg atgagaaggc ggaaccggtt gtgctggact ggcgggcacc
      841 gatttcctcc gtctattatg agaacagcac cgggccgtgc agctatacgg tgagttcaga
      901 gggtactttt tcgattgatc tgaaaagaaa gcggacgtat gagattgaaa atgaccatct
      961 gaaagacttt tttgattccg atgtggtagc gaacgatgag ctgctgacaa agtatctggc
     1021 gaagaacaaa aaagcggtgc tcggtgagat cattgcgacg atccagcagg agcagaacct
     1081 cattatccgc agatccccga agacgaatct gatcgtacag ggcgtggctg gttccggaaa
     1141 gacgacggtg gcgatgcacc ggatttcgta catcctctat aattacgagg aggacttccg
     1201 gccggaggat ttctatatca ttggaagcaa ccgcattctg ctgaactaca ttaccagcgt
     1261 tctgccggaa ctcgatgtgt acggcatccg tcagatgacg atggagcagc ttttcatccg
     1321 gcttctgtac gaggactggg acgaagaaaa gtacatggta cacgccatcg accgcgcgga
     1381 tgagaaaaac agcataaagg gcggaagcgg ctggtttttc gatctggaaa atttctgccg
     1441 tacgtacgag gcagagcaga ttccgcggga gccggtgcgg ctggaaaaaa cgggaacatt
     1501 gcttctggac gccgagtata ttgataacta ttgcagggag cagtccacac tttccatgga
     1561 aggaaagatg tgcatgctga acgagatcct gctggcaaaa tttgagaacg aggtttcggg
     1621 aaaagaagtg acgtttccgg cgcgcgaaaa aaaggcgctg aagcggaaat acgagaaata
     1681 ttttggagat ggaaaatggc ggggatccat ttttgatctc tatttacagt ttctggagca
     1741 gcaggcggag aaaggaaaag cggtggaagt gccggaaaac tcctttgatg tctatgatct
     1801 ggcggcgctg gcatacctct ataagcggat caaggaaaac gatcccgtgc gcgaggcgag
     1861 ccatgtggtc atcgacgagg cgcaggattt cgggatgatg gcttaccagg tgcttcatta
     1921 ctgtctgcgt gactgtacgt ataccattat gggagatacg tcgcagaata tccatttttc
     1981 ctacggcctg aacgactggg aggagctgaa aaagctgatt ctgaccggca cgtacgatgc
     2041 gttcggcgtt ctgcggaaga gttaccggaa tacggtggag atctccgatt ttgcaaatga
     2101 aatcctgcgg cacggggatt ttgcgattta tccggtggag ccggtgctgc ggcacggaac
     2161 ggcggtgcgg aaagaagcat ttgacgatga agcggcgctg ctcgccgcgg gtgttcagac
     2221 aatcaagaca tggcaggcgc agggatatga gacgattgcc gttgtatgcc gggatgaggc
     2281 agaagcggcg gatacggcgc ggaagctgaa acaatacgtg ccggttgtgg aggaggatct
     2341 cgagacggca gagttcggcg agggcgtgat ggtgctcccg gtggcatata cgaaaggtct
     2401 tgagttcgat gcggtgctgc ttcttgatcc gacggaagaa aaatatcctg aaaatgacgg
     2461 gcaggtgaag ctgctttacg tggcggcgac gcgggcactg catgagctgg cggtgctgta
     2521 tacaggagaa ctgacgggaa tccttgccaa agaagcgccg aagggccgtc acaatcagga
     2581 atttgccatg gagacgctga cgaaggcgaa agagtatgag aaggtttcgc tgacgcagaa
     2641 ggaggcgcgt gaggagaacc gcgcgatcgg cattcaggag atggacgagc ggaacagcca
     2701 tggaccgaaa cggattgtga tcaagccgga gcagaggccg ggaatggcgc ttggaaatac
     2761 aagcacgacc ggaacagcgg tgatggcaaa aggcagtacg gcaacgcgga gggctgcggc
     2821 agaaacggga gaagaaattg ccgcaaagac aatgcagcag cgtgcaccgg aaggggtgtc
     2881 atctattttt gctggtgcgg cttatcatgg aaaggcaggc ggcaaagcgc cgggaagcag
     2941 acggagtgca ggaacacagg caggaatgca atatggaacg ggcacggccg gagtgcagag
     3001 tgcaaaaaga ccgggcggag caggtatggt accaggagaa gccgagaaga cggagcgcct
     3061 gaatacctca ccttacgctt tcggtgcaat cccggaaaac gagctgctgc gcatcaaggg
     3121 ccattcgaag atcaagtgtg cggtgaaatg ggcgaagaaa ggaaaatcgg cggttgagat
     3181 tgccagcatg tacggcatct tacgcatcac cccgatcacg ccggaggtga tccgcatcag
     3241 tttcgtaaag ggtgtgaccg caaaagtgca ggacacgtac tggaagccga aggcagacac
     3301 cgcgtttccg tggagtgcga aagaatcgaa gacggcggtt ctggtcgaga cggaaaagct
     3361 gcgcgtcatg gtagaaaaaa aggacggcgc ggtgcagttc ctgacgccgg acaacaaacc
     3421 aatcctttcc gagaagcgtg acgagccgcg catgatcgac ggcggcatga catggacgtt
     3481 cttcgactgg agcggttccg aaaagctgaa agcaaaaggt attctttcga ccgagtggct
     3541 ggatctgacc gcgaaggcgc gctacgtctc ttttggcgga aagcaggcgc ggatgccgct
     3601 gcttgtttcg aaccgcggct acggcattgc ggccgccgcg tcccggacgg cactgctgtg
     3661 caatgtacgg acgtttggaa cgtatcttca caccgcaggg gacggacaga ttgattatta
     3721 cttcattctc ggaaaagacc gggaggaaat tgtaaaacag tataaagagc tataaaagga
     3781 gcagacattt tatgaaaact ctggtaattg cagaaaaacc atccgtggca cgcgacattg
     3841 cgcgcgtact ggggtgcagc aaaaaagaaa acggagcgct ggagggaaac aagtatatcg
     3901 ttacctgggc gctcggccat ctggtgacgt taaaagatcc ggaagactat gacaaaaagt
     3961 ataaggaatg gaatatggca gttctgccga tgatgccgga aaaactgcag atcaaggtca
     4021 ttccgcagac gggaaaacag taccatgcgg tgacgacaca gcttgcgcgg aaggacgtga
     4081 atgagattgt cattgcaacc gatgccggac gcgagggtga gctggtggcg cggtggattc
     4141 tcgacaaagc acataaccag aagccgtgta agcgtctctg gatttcctct gtgacggaca
     4201 aggcgatccg tgagggtttt gccaatctga aggatgcgaa agagtacgac aatctctacc
     4261 gcgctgccgt ttcccgtgcc gaggcggact ggctcgtcgg catcaatgcg acgcgggcgc
     4321 tgacctgtaa atacaatgca cagctttcct gcggccgtgt gcagacgccg acacttgcca
     4381 tgatcgcaca gcgcgagcag gaaatccgtg aatttgtccc gaaaccgtat tacacggtga
     4441 cggccgcggc gggcggtgtg gtctacacct ggaaagatga aaaatcgggc ggcacaagaa
     4501 tctccgatcc ggaaaaggca aagcagatcg cagagaaggc aaaacggtat ccgcttgtct
     4561 tacagaacgt agaaaagaag aaaaagcaga aagaagcgcc attgctctac gatctgacgg
     4621 agctgcagcg cgatgcgagc gcacggttcg gctattcggc aaaacagacg ctgaatctga
     4681 tgcagagcct ctatgagacc cataaagtcc ttacgtatcc gcggacagat tcacggtatc
     4741 tgacgaaaga tattgtgccg acgttaaaag agcggctgga tgcggtcagc atcggcccgt
     4801 acaaagcact ggcccagcag gcgaagcgca gtccgctgaa cggaaagctc ttctttgtca
     4861 atgatgcgaa ggtttccgat caccatgcca ttatcccgac ggagcagtac gtgcagttat
     4921 ccgcactctc gaacgaggag cgccgcattt atgatcttgt tgtgcgtcgt tttcttgcgg
     4981 tactgctgcc gccgtgtgta tatgaagaga cggtgatcca ggcttccatc gaaaaggaat
     5041 gctttactgc gcgcggaaag cgcatggtcg agaaaggctg gcaggaggcg tacgaggata
     5101 accggtacga cgaagaggat gaggcgaaag aagaggtgcg ggagcagaat ctgccgcttc
     5161 tggaggcagg catgaagatc gggcagccga agatcagtct ccgcgaagga aagacaaaac
     5221 ctccggcacg cttcacggaa ggcacgctgc tctccgcgat ggaaaatccg gtccgctaca
     5281 tggaaaaccg cgacagcagc ctggtaaaaa cgatcggtga ggcgggcggc cttggaacgg
     5341 ttgcgacccg tgcggatatc atcgagaagc tgttccgcag ctttctgctg gagaaaaaag
     5401 gcaatgagat ctatctgacc tcgaaagcac gccagcttct gaagctggtt ccggcggatt
     5461 tgaaaaagcc ggagctgacg gcaagctggg agatgcagtt aaacgacatt gcaaagggaa
     5521 agaagcggcg cgatgtgttt atgaaggaga tccgcagcta cacggtggaa ctgatcgatg
     5581 agatcaagac ggaagaggga acgttccgcc acgacaacct gacgaacaag aagtgcccga
     5641 actgcggaaa acggctgctt gcggtgaatg gaaagaacgc aaaactgctc gtctgccagg
     5701 acagagagtg cggctaccgc gagacggttt cccgcacgac gaatgcgaga tgcccggtct
     5761 gccataagcg catggagatg atcggaaagg gcgaggatgc gacgtttgtc tgcgcctgcg
     5821 gccacaaaga gcgcatgaca aaattccaag agcgccgcaa aaaagagggc ggcggcgtga
     5881 cgaagcgcga tgtggcagct tatatgaaga aacagaaaaa agaggccgaa gagccggtga
     5941 acaatgcgtt tgccgcggca ttaaaaggaa ttaaattata aaaaagggag cgagttctgc
     6001 cgaaaatcaa aaaacggcgg aacccgcttc ttttgttaaa atcgttgagg aaaaggtcaa
     6061 aaaaagtgat tgttttatgc agagttatgc cgtaaaatag aaacaaatga tataagaaac
     6121 aggaaaattc tgaaaataac aacagggagg tttttcaatg aaacagacag atctggaaaa
     6181 actgctggcg gatatgtcgc tgaaagagaa agtagatcag atgcttcagt taagcggagc
     6241 attttacctc ggggatgaaa acagcgtcct gaccggcccg gcaaatgaca tgggaatcgg
     6301 aaaagaggac ctggagatgg ccggttccat tctcggtgca gccggtgccg atacgatgaa
     6361 aaaactgcag gatgactaca tggcaaaaca gccgcatcac atcccgatgc tgttcatgat
     6421 ggatgtcatt cacggcatga agaccatttt cccggcgccg ctggcgcagg gagccacttt
     6481 tgatccggag ctctccggcg agtgcgcgtc tgcagccgca aaagaagcat ccgcggccgg
     6541 acttcacgtt accttttctc cgatggttga ccttgtgcgc gatgcgagat ggggccgcgt
     6601 tgtggaatcc acaggcgaag atccgtacct gaacagccgc ttttccgagg cgatcgtaaa
     6661 aggcttccag ggcgatgatt taaaaacacc gggaaaagtt gcctcctgca tcaaacactt
     6721 tgcaggctac ggcggagcag tcgcaggaag agattacaac accgtagagc tctcagagca
     6781 taccttccgt gaattctatc ttccggcata caaagcaggc atcgatgcgg gcgcagcgat
     6841 ggtgatgaca tcattcaaca cgatcaacgg cgttccggcg agcaccaaca aatggctgat
     6901 gcgcgatatc ctgcgcggcg agatgggatt tgacggcgtg ctgatttctg actttgcagc
     6961 cattctggaa accgttgccc acagaagctc gaaagatgcc gccgatgcag cgaaaaaagc
     7021 gctggaggca ggcgtggata ttgatatgat gacatccgtt tacgccgcaa acctctgccg
     7081 tctcgtagaa gaaggtgaag tggacgagca tctgattgac gagtgctgcc tgcggattct
     7141 ggagctgaaa aacaaactgg gactgtttga aaatccatat aaagatgcgg atgccgagaa
     7201 agagaaagca tacaacctct gcccggagca ccgtgcactg gcgaaaaaag cagcggaaga
     7261 atccttcgtc ctgctgaaaa acgacggcgt actgccgctc gacactgcga aaaagatcgc
     7321 atttgtcggc ccgtatacca acaatcatga aatcaagagc agctggtcct tcaccggcga
     7381 cagcaaggac tgcgtgacga ttcaggaagc agccgagaaa gtcttcgacg catcacgcac
     7441 cacgtatgca gaaggatgcc cggttatcgg aaacgatgtg gagctgatcg gatttaccga
     7501 gacgacaccg aaaaaataca gtgaggaaga gcttgctgcc atggagcaga gcgcactaca
     7561 ggcagcaaaa gaggccgatc tcgtggtaat gccgatgggt gagcattatc tgcagagcgg
     7621 cgaggcgacc agccgtgcca tgatcgatgt gccggaaatc cagatggagc tgttccgcaa
     7681 agtgctggcc gtcaacccga atgtggttgt cgttctgttc aacggccgcc cgctggatct
     7741 gcgggaaatc agcgctaaag caaaagccat tctcgaggta tggttcccgg gaaccgaggg
     7801 cggaagcgct gttgtggatg tcctgaccgg aaagaaaaat ccgtctggca agctgccgat
     7861 gagcttccca tactgcgtgg gccaggcacc ggtcagctac aacgagtatt ccaccggaag
     7921 accgaatctg ccgggcatga aagagcggtt ccgttccaaa tatcttgaca ttccgaatgc
     7981 accgctgtat ccgttcggct atggtctttc ctacacgaca tttgccgttt cggatgtcgt
     8041 gcttgaccag aacgagatgg ataagaatgg cagaattgca gcaaaagtaa ccgtgaagaa
     8101 taccggtgat gctgcaggct gcgaggttgt tcagctgtac atccgcgatg attattccga
     8161 tgtggttcgt ccggtgaaag agctgaaagg tttccggaaa gtagagcttg ccccgggtga
     8221 ggagcaggag gttacgttct atattaccga agaggatctg cgctactacc ggctcgacgg
     8281 aaccttcggc agcgagccgg gtacctttac ggtgatgatc ggcaacagca gcacgacgga
     8341 aaacagtgca gtatttactt taacaccagt aga
//
LOCUS       DAOMUF010000086         8339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2212_length_8339_cov_2.620232, whole genome shotgun sequence.
ACCESSION   DAOMUF010000086 DAOMUF010000000
VERSION     DAOMUF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2212_length_8339_cov_2.620232"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLN77_06220"
     CDS             <1..274
                     /locus_tag="PLN77_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRM43907.1"
                     /translation="GGRKNTFLYSVNQEAAREIARQIRLRGISGLILVDFINMPEKEQ
                     EERLLGFLQGLVREDPVPTKVVDMTALHLVELTRKKIRRPLAEQMK"
     gene            302..1297
                     /locus_tag="PLN77_06225"
     CDS             302..1297
                     /locus_tag="PLN77_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRM43908.1"
                     /translation="MEPQKKPIHLNENDTPYLYEPFRNQMPAKRPHPAEKEKILKPWQ
                     GLLVFAFLMVLFNLAGIPLVLAGGMYGNALDEIIVFLIGSILVVRALHIPLKEVFPLK
                     KPDGAGILGTILMWYVTYRGVLALFLLMEWIFPQEYASLSESMDSSMAGLSYVVALTP
                     AICEEALHRGLLQYSLRGIKKKWVMLLLMGVYFGAFHMSIVRFLPMMMMGIVLSYVRM
                     KTDNMFYSSLFHFVHNASTIVASIAASFLYMFSQGNAAAGSGYATETYIGMYLIEAAL
                     MPIGLYMGSRLLMYQQTKSEGFFPKERRKKTIAKIVIPTILLILGGLFFLGIGMM"
     gene            1454..2329
                     /locus_tag="PLN77_06230"
     CDS             1454..2329
                     /locus_tag="PLN77_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM43909.1"
                     /translation="MARTAIATDSNSGITQSQAKELGVFVLPMPFFMRGKIQFEDIDL
                     TQEQFYEILAEDAAVSTSQPSPGDLMDFWNKILEEYDDIVYIPMSSGLSTSCDTATVL
                     SEDYEGKVQVVNNQRISVTQQQSVLDAKKMANRGVAAKDIKAILEEDALDASIYIMVD
                     TLKYLKKGGRVTPAAAMIGTVLNIKPVLTIQGEKLDAFEKARGVKQAKKAMIKAMKKD
                     MDKRFAKERAAGIMGLHIAYTANDKEVIDGWIVEVKEAFPDFEVDAHPLSLSVACHIG
                     PGALAIACEKKSAIS"
     gene            2446..2751
                     /gene="rplU"
                     /locus_tag="PLN77_06235"
     CDS             2446..2751
                     /gene="rplU"
                     /locus_tag="PLN77_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRM43910.1"
                     /translation="MYAIIATGGKQYKVAEGDVIRVEKLGVEAGETVTFDQVLAVSND
                     GLKVGEDVKNASVTASVVGNGKGKKVIVYKYKRKTGYHKKNGHRQQYTEVKIEKINA"
     gene            2757..3077
                     /locus_tag="PLN77_06240"
     CDS             2757..3077
                     /locus_tag="PLN77_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRM43911.1"
                     /translation="MICITVTESDGEYISVESNGHAGYAEEGQDIICAAVSALIVNTV
                     NSVETLTEDLIISEAGDGYVYFSFPNGYSESTALLVKSLLLGLESIRRDYGAQYLEIA
                     FKEV"
     gene            3082..3372
                     /gene="rpmA"
                     /locus_tag="PLN77_06245"
     CDS             3082..3372
                     /gene="rpmA"
                     /locus_tag="PLN77_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRM43912.1"
                     /translation="MLNMNLQIFAHKKGVGSTKNGRDSESKRLGAKRADGQVVKAGNI
                     LYRQRGTKIHPGVNVGIGKDDTLFAMVDGRVAFERKGRDKKQVSVYPVATEE"
     gene            3483..4781
                     /gene="obgE"
                     /locus_tag="PLN77_06250"
     CDS             3483..4781
                     /gene="obgE"
                     /locus_tag="PLN77_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRM43913.1"
                     /translation="MFADRAKILIRSGKGGDGHVSFRRELFVPNGGPDGGDGGKGGDV
                     IFEVDEGLNTLVDYRHRRKFSATDGEPGGKRRCHGGNGADIVLKVPEGTVIMDAASGK
                     VIADMSGENRRQVVLKGGKGGNGNMHYATATMQVPKYAQPGQPAQELEVRMELKVIAD
                     VGLVGFPNVGKSTLLSRVTNAEPKIANYHFTTLSPNLGVVDLEGCSGFVIADIPGLIE
                     GASEGVGLGHEFLRHIERTRVMIHVVDVASTEGRDPVDDINKINKELEAYNPEIAKRP
                     QIIAANKTDAIYVEEGEEDPVERLKKEFEPKGIPVYPISAVSGKGLKELLYAVQKKLD
                     ELPKERMVFEQEFFPEDMLLAENLPYTVEQDPEDEHRFIIEGPKIEKMLGYTNLDSEK
                     GFAFFQRFLKDTGILDELEQAGIQEGDTVKMYGFEFDYYK"
     gene            4810..5103
                     /gene="yhbY"
                     /locus_tag="PLN77_06255"
     CDS             4810..5103
                     /gene="yhbY"
                     /locus_tag="PLN77_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HRM43914.1"
                     /translation="MTSKQRAYLKGLAMTMEPILQVGKSSITPEHTAAVAEALEAREL
                     IKINVLQNCLDDPREIAEVLAERTNSQVVQVIGKKIVLYKEGKKEKKKIQLPK"
     gene            5171..5791
                     /gene="nadD"
                     /locus_tag="PLN77_06260"
     CDS             5171..5791
                     /gene="nadD"
                     /locus_tag="PLN77_06260"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRM43915.1"
                     /translation="MEKTNRKIGIMGGTFDPIHIGHLILGEAAYEQFGLDEVWFLPAG
                     NPPHKRNRAGRAKDAQRVEMVRRAIASNPHFVLCTKEMEDEGYTYSYRTLEAMRKEHP
                     GTEFYFIIGADSLFYFDEWKNPERICAAAKILVATRDRAKEKELLACMERNAKKLHGT
                     FLKLDTPNLDVSSHELREWIREGKSVKYYLPDSVISYIQEQKIYQD"
     gene            5804..6406
                     /gene="yqeK"
                     /locus_tag="PLN77_06265"
     CDS             5804..6406
                     /gene="yqeK"
                     /locus_tag="PLN77_06265"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HRM43916.1"
                     /translation="MEQYDLWKLDKKLQKEVDEDRYHHTLGVMFTAASMAMVWGEDLE
                     KARVAGLLHDCAKCIPNKKKITMCEKNKIEITEFERNNPFLIHAKLGAFLAKDKYKVE
                     DPEILSAITWHTTGKEDMTLLEKIIYIADYIEPARNKAPHLTRLRKLAFTDIDECMYE
                     ILKDSMAYLSENPKTMDPMTEQAYQFYEALHRERTAKEEA"
     gene            6407..6760
                     /gene="rsfS"
                     /locus_tag="PLN77_06270"
     CDS             6407..6760
                     /gene="rsfS"
                     /locus_tag="PLN77_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRM43917.1"
                     /translation="MSEAKQTAKLALEALEDKKAMDVRILDITHVSTLADYFMIASGS
                     NRNQVQAMADNVEEVLGKAGVHPKQIEGYQTANWILMDYGDVVIHIFDEENRLFYDLE
                     RIWRDGKVVEKEEIM"
     gene            6877..>7342
                     /locus_tag="PLN77_06275"
     CDS             6877..>7342
                     /locus_tag="PLN77_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43918.1"
                     /translation="MKKIESGSLAVMLDEETLGLRIEKDGVVWNTDPKKTPELIFAEG
                     TYRFLDAEEISHEVYSFGVGCGIRSRYAGFAGTPYAFETIIWIEAVDETLWMEWIPLC
                     EEGLHPVKVLWPTAMEFTNGRDDWYTLLTEGQGLLIPNTWKTELGKLSFDGRF"
     assembly_gap    7343..7352
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7353..>8339
                     /locus_tag="PLN77_06280"
     CDS             <7353..>8339
                     /locus_tag="PLN77_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HRM43919.1"
                     /translation="YMPWFGQVKERCGYTAICTTPWNAGYQAEHPAGGPYTSVGAWLE
                     PSLGTMNYRRVFRYTFLNDCDYNDLCKAYRQYVREQGHLRTLKEKAVQNPSIHDLVGT
                     CFVHTGIKTVVQPESGFFDPQNPEKNNRVVPFSVREQQIREIHDLGVEKVYLHLDGWA
                     EPGYDNQHPDYTPACQAAGGWEGMKSLVDTMHDFGYKFGIHDQYRDYYHAAPSYDENY
                     ACRLPDGTIPGHSYWAGGPQSYLCATQAPFYVKRNFAELKKNGIRLDGAYLDVFTCNE
                     GDECANPEHVMTRRDCYMYRGNCFSWLLSQGILSSSEEVSDWAVPYLVFCHYA"
BASE COUNT         2431 a         1835 c         2264 g         1799 t
ORIGIN      
        1 cggaggacgt aaaaatacgt ttctgtacag cgtcaaccag gaggcagccc gcgaaatcgc
       61 ccgccagatc cgcctgcgcg ggatttccgg tctgatcctg gttgacttta tcaacatgcc
      121 ggaaaaagag caggaggagc gccttctcgg cttcctgcag ggtcttgtgc gggaagatcc
      181 cgttccgacc aaagttgtgg atatgacggc gcttcatctc gtggaactga cgagaaagaa
      241 aatccgccgg ccgctggcag aacagatgaa atgacggtaa ccttacaaag gaggaatgaa
      301 tatggaacca caaaagaaac cgattcacct caatgaaaat gataccccat atctttacga
      361 accctttcga aaccagatgc cggcaaaacg gccgcatccg gcggaaaaag aaaagatact
      421 gaaaccatgg cagggcctgc tggtgtttgc ctttctgatg gttcttttta atctcgccgg
      481 aatcccgctg gttcttgcag gcgggatgta tggaaatgcg ctggacgaga tcattgtatt
      541 cctgatcgga tccattctcg tcgtacgggc acttcatatt ccgttaaaag aagtgttccc
      601 attaaaaaag ccggatggag ccggaatcct cggaaccatt ctgatgtggt atgtgacgta
      661 ccgcggtgtg cttgcccttt tccttctgat ggaatggatt ttcccgcagg aatacgcaag
      721 cctctcggaa tccatggaca gcagtatggc gggcctttcc tatgtcgttg cgctgacacc
      781 ggccatctgt gaagaagcac tgcaccgcgg tcttctgcag tacagcctgc gcggaatcaa
      841 aaagaaatgg gtgatgctgc ttctgatggg cgtttatttt ggcgctttcc acatgagcat
      901 cgtccgtttt cttccgatga tgatgatggg aatcgttctc tcgtatgtgc ggatgaagac
      961 cgacaacatg ttttacagct cgctgtttca tttcgtccat aacgcatcga cgattgtggc
     1021 gagcattgcg gcaagcttcc tctatatgtt ctcgcagggg aacgcagcag caggcagcgg
     1081 atacgcaacg gaaacgtaca tcggcatgta tctgatcgaa gcggcgctga tgccgatcgg
     1141 tctttatatg ggaagccgtc ttctgatgta ccagcagaca aaaagcgaag gatttttccc
     1201 gaaggagaga agaaaaaaga ccattgcaaa aatcgtaatt ccgaccattc tgctgatcct
     1261 tggcggactg tttttccttg gtattggtat gatgtaaaaa gaatcaaaag cagtttgggc
     1321 aattttgcag aaatgtccaa gccgggtaga aaacaggtac gatttatgat agaataacag
     1381 caaatcaggc gttacacgat attacagaaa ggaaaagctg tttctctaac aagagacagc
     1441 aggaataaga actatggcaa gaacagctat tgctaccgac agcaacagcg gtattacaca
     1501 gagtcaggcg aaagagctgg gcgtttttgt ccttccgatg cctttcttca tgcgcggaaa
     1561 aatccagttt gaggatatcg acctgacaca ggaacagttt tacgagattc tggcagagga
     1621 tgcggctgtt tccacatctc agccgtctcc gggagatctg atggatttct ggaacaaaat
     1681 tctcgaagag tacgatgaca ttgtttacat tccgatgtca agcggccttt ccacctcctg
     1741 tgatacagcg accgtgctgt ccgaggacta cgaaggaaaa gtgcaggttg tcaacaacca
     1801 gagaatctcc gttacgcagc agcagtccgt tctggatgca aagaaaatgg caaaccgcgg
     1861 cgtggcagca aaagacatca aagcaattct ggaagaggat gcgctggatg caagtatcta
     1921 tattatggta gatacgttaa aatacctgaa aaaaggcggc cgcgttacgc cggcagcagc
     1981 catgatcgga accgttttaa atatcaaacc ggtgctgacg atccagggag agaagcttga
     2041 cgcgtttgaa aaagcgcgcg gtgtgaaaca ggcaaagaaa gccatgatca aggctatgaa
     2101 aaaagatatg gacaaacgct tcgcaaaaga gcgtgcagcc ggcatcatgg gacttcacat
     2161 tgcctatacc gcaaacgaca aagaagtaat cgatggatgg attgtcgaag taaaagaagc
     2221 cttcccggat tttgaagtcg atgcgcatcc gctgtcctta agcgttgcct gccacatcgg
     2281 acctggcgca ctggcaattg catgtgaaaa aaaatctgca atttcctgaa aaatactatt
     2341 gacaaacagc agacgtgatg atatattgta gaagtatgcc gcacagagag gtaaaagagt
     2401 cgaaagacca ccgaatctgg cgagtaattg atagggaggt gccatatgta cgcaattatt
     2461 gcaacaggtg gtaaacagta caaggtagct gaaggcgatg ttatccgagt agagaaactt
     2521 ggcgttgaag ctggagaaac cgtaactttt gatcaggtac ttgcagtaag caatgacgga
     2581 ctgaaagttg gcgaggacgt taaaaatgca tccgtaaccg catctgtagt tggaaacggt
     2641 aaaggcaaaa aagttatcgt ttacaagtat aagagaaaaa ctggttatca taagaaaaac
     2701 ggtcacagac agcagtacac tgaagtgaag attgaaaaaa tcaacgctta agtcttatga
     2761 tttgtataac cgtaacagag tctgacggcg agtacatctc cgtggagtca aacggtcatg
     2821 caggctatgc agaggaaggg caggatatca tctgtgccgc tgtctccgca ttgattgtaa
     2881 acaccgtaaa ttccgtggaa acattgacag aggatctgat tataagtgag gctggagatg
     2941 gatacgtgta tttttcattc ccgaatgggt attctgaaag taccgcactt cttgtaaaat
     3001 cacttttgct gggcctcgaa agtatccgcc gcgattatgg agcacaatac ttagagatcg
     3061 cattcaagga ggtgtaatac catgttaaat atgaaccttc agattttcgc tcataaaaaa
     3121 ggagttggtt ctaccaagaa cggtagagat tccgagtcca agagattagg agcaaaaaga
     3181 gctgacggac aggttgtaaa ggctggaaac attctttaca gacagcgtgg aaccaagatt
     3241 catccaggcg tgaacgtagg aatcggaaaa gacgatacct tattcgctat ggttgacgga
     3301 cgcgtagctt tcgaaagaaa aggacgtgac aaaaaacagg tttccgtata tccggtagca
     3361 acagaagagt aagacaaagg tgcggcttca aatcttaaga ggtttgaagc cgtttttgtt
     3421 aataataatt aaaggtccaa cagaccgatt tggatttaga taaaacaaga caggagaata
     3481 ccatgtttgc agacagagca aaaattctga tccgctccgg taagggcgga gacggacatg
     3541 tcagtttccg cagagagctt tttgtaccga acggcgggcc ggacggcggc gatggcggca
     3601 aaggcggaga cgttattttt gaagttgacg aaggactgaa tacactggtt gattaccgcc
     3661 acagaagaaa gttttctgct accgacggcg agccgggcgg aaaacgcaga tgccatggcg
     3721 gaaacggcgc agatattgtc ctgaaagtac cggaaggaac cgtcattatg gacgctgcga
     3781 gcggaaaagt catcgcagat atgtccggtg aaaaccgccg tcaggttgta ttaaaaggcg
     3841 gaaaaggcgg aaacggaaat atgcattacg caaccgctac catgcaggtt ccgaaatacg
     3901 cccagccagg ccagccggca caggagctgg aagtaagaat ggaactgaaa gtcatcgcgg
     3961 acgtcggact cgtcggcttc ccgaacgttg gaaaatcgac cctgctttcc cgcgtgacaa
     4021 atgcagaacc gaaaattgca aattatcact ttaccacgct ttccccgaac ctcggtgtcg
     4081 tagaccttga gggctgcagc ggatttgtta tcgcagatat cccgggactg atcgagggtg
     4141 catcggaagg agtcggcctc ggccatgaat tcctgcgcca catcgagaga acccgcgtga
     4201 tgatccatgt agtagacgtt gcatcgaccg agggaagaga tccggtggat gatatcaaca
     4261 agatcaacaa agagctcgaa gcctataacc cggagatcgc aaaacgcccg cagatcatcg
     4321 cggccaataa gaccgatgcg atctatgtag aagagggcga agaggatccg gtggaacgtc
     4381 tgaaaaaaga gttcgagccg aaaggcatcc cggtttatcc gatttctgcc gtcagcggaa
     4441 aaggcttaaa agagctttta tatgccgtac agaaaaaact cgatgagctt ccgaaagagc
     4501 gtatggtctt cgaacaggaa ttcttcccgg aggatatgct tcttgccgag aacctgccgt
     4561 acaccgtcga gcaggacccg gaggacgagc accgcttcat catcgaggga ccgaagatcg
     4621 agaagatgct cggctacacc aatctggatt ccgagaaagg ttttgctttc ttccagcggt
     4681 tcttaaaaga taccggtatc ttagacgaac tcgagcaggc cggcatccag gaaggcgata
     4741 ccgtcaagat gtatggattt gaatttgatt attataaata gaacgaataa ataacgaagg
     4801 aggagacgta tgaccagcaa gcagagagca tacctgaaag gccttgccat gaccatggaa
     4861 ccgatcctgc aggtaggaaa aagcagcatc acgccggagc ataccgctgc tgtagcagag
     4921 gcacttgagg cgagagaact gattaaaatc aatgtactgc agaactgcct cgatgatcca
     4981 agagagatcg cagaagttct ggcagaacgc accaattccc aggtcgtaca ggtgatcggc
     5041 aaaaagatcg tgctgtacaa agagggcaaa aaagagaaaa agaaaattca gcttccgaaa
     5101 taatccgcag gaaagaaccg ccgggaccgg agcataccgc tgcagttccg gcgctttttg
     5161 aggaaaacgt atggaaaaga caaacagaaa aattggcatt atgggaggca ctttcgatcc
     5221 cattcatatc ggtcatctga tccttggcga ggcggcgtac gagcagttcg ggcttgatga
     5281 agtctggttc cttccggccg gaaatccgcc acataagcgg aaccgggcgg gacgggcaaa
     5341 ggatgcacag cgtgtcgaga tggtgcgccg tgccatcgca tccaatccgc attttgtgct
     5401 ctgcaccaaa gagatggaag atgagggcta tacctacagt tatcggacgc ttgaggcgat
     5461 gcggaaggaa catccgggca cggagtttta ctttatcatt ggagcggatt ccctgttcta
     5521 ttttgatgag tggaaaaatc cagagcggat ctgcgccgcg gcaaagatcc ttgtggcgac
     5581 cagggatcgg gcaaaagaga aagagcttct ggcctgtatg gaacgaaatg caaaaaaact
     5641 tcacggtacc tttctgaaac tcgacacacc gaatctggat gtttcttccc acgaactgcg
     5701 ggaatggatc cgggaaggaa aatccgtgaa atactatctg ccggattctg tgatttccta
     5761 cattcaggag cagaaaatct atcaggatta gaggaagagc aggatggaac agtacgattt
     5821 atggaaactg gacaaaaaac tccagaagga agtcgatgaa gaccgctatc atcacacgct
     5881 cggtgtgatg tttaccgcag catccatggc gatggtatgg ggcgaggatc tcgaaaaagc
     5941 ccgtgtcgcg ggactgcttc acgactgtgc aaaatgcatt ccgaataaga aaaaaatcac
     6001 catgtgtgag aaaaataaga ttgaaatcac tgagtttgag cggaacaatc cgtttttgat
     6061 ccatgccaag ctcggtgcat ttctggcgaa ggacaagtat aaggtggaag atccggagat
     6121 cttaagtgcc atcacctggc atacgacagg aaaagaagat atgacattgc tggagaagat
     6181 catctatatc gcagattata tcgagccggc acgaaacaag gcaccgcacc ttacccgcct
     6241 tcggaagctg gcattcaccg atatcgacga atgcatgtat gaaattttaa aagacagtat
     6301 ggcgtatctg tcggaaaacc cgaagacgat ggatccgatg acggagcagg catatcagtt
     6361 ctatgaagca ctgcaccggg aacggacggc aaaggaggaa gcataaatga gcgaagcaaa
     6421 acagactgca aaacttgcac tggaagcact ggaagataaa aaagcaatgg atgtccgcat
     6481 tctggacatt acccatgttt cgactctggc agattatttc atgatcgcca gcggaagcaa
     6541 ccgcaaccag gtacaggcga tggcagacaa cgtagaggag gtactcggaa aagccggcgt
     6601 acatccaaaa cagatcgaag gctatcagac cgcaaactgg attctgatgg actacggcga
     6661 tgttgtcatc cacatctttg atgaggagaa ccgcctgttc tacgatctgg agagaatctg
     6721 gagagacggt aaagtcgtag aaaaagaaga gattatgtaa gttaggtgga aaaaggcgca
     6781 gaaaatccgg aaccgtgcag agaaaggcgg acgggttctc tgcgcctttc tggtgtttgg
     6841 aacgcatatg ataaagaaaa aggggaatat cacacgatga aaaaaataga aagcggatcg
     6901 cttgctgtga tgctcgatga agagacgctg ggacttagga tcgaaaaaga cggcgttgtc
     6961 tggaatacgg atccgaaaaa gacaccggaa ctgatttttg cagagggaac ataccgtttc
     7021 ctggatgcag aggagatttc ccatgaggtc tattccttcg gcgtcggatg cggcatccgg
     7081 agccgttatg cgggatttgc gggaacaccg tatgccttcg agacgattat ctggatcgaa
     7141 gctgtggatg aaacactctg gatggagtgg attccgctct gcgaggaggg actgcatccg
     7201 gtaaaggtgc tgtggccgac ggcaatggag tttacaaacg ggcgcgatga ctggtataca
     7261 ctccttaccg agggacaggg acttctgatt ccgaatacct ggaagacgga acttggaaag
     7321 ctttcgtttg acggcagatt tgnnnnnnnn nntatatgcc gtggttcggc caagtgaagg
     7381 aaagatgcgg ctatacagcg atctgtacaa caccgtggaa cgccggttat caggcagagc
     7441 acccggcagg cggcccgtat accagcgtgg gggcatggct ggagccgagc cttggaacaa
     7501 tgaattaccg ccgcgtattc cgttatacgt ttttaaatga ctgcgattat aacgacctct
     7561 gcaaagcgta ccgccagtat gtacgggaac agggtcatct gcgcactttg aaagaaaagg
     7621 cagtacagaa tccgtccatt cacgatcttg tgggaacgtg ttttgtccat acgggaatca
     7681 agacggttgt gcagccggag tccggctttt tcgatccgca gaatccggag aaaaataacc
     7741 gcgtagtgcc gttttccgtg cgcgagcagc agatccgcga aattcatgat ctcggcgtag
     7801 agaaagtcta tcttcatctg gacggctggg cagaaccggg gtatgacaac cagcatccgg
     7861 actacacacc ggcatgccaa gcggccggcg gatgggaagg catgaaatcg cttgtcgata
     7921 cgatgcatga tttcggctac aaattcggta ttcacgacca gtaccgcgat tattaccacg
     7981 cagcgccgag ctacgatgaa aattatgcct gccgccttcc ggacggaacg ataccgggac
     8041 acagttactg ggcgggcgga ccgcagagct atctctgtgc aacgcaggca ccgttctatg
     8101 tgaaacggaa ttttgccgag ctgaagaaaa acggaatccg gcttgacggt gcatacctcg
     8161 atgtgtttac gtgcaacgag ggcgacgagt gcgcaaatcc ggagcatgtg atgacgagaa
     8221 gagactgcta tatgtaccgc ggaaactgct tctcctggct gctctcacag gggattctct
     8281 caagctccga ggaggtcagc gactgggcgg ttccgtatct ggtattctgc cattatgcg
//
LOCUS       DAOMUF010000087         8302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2227_length_8302_cov_3.498121, whole genome shotgun sequence.
ACCESSION   DAOMUF010000087 DAOMUF010000000
VERSION     DAOMUF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2227_length_8302_cov_3.498121"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLN77_06285"
     CDS             <1..950
                     /locus_tag="PLN77_06285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM43920.1"
                     /translation="YQAASESGLWYPAAKPGDMVAEGALLGTIRDYNGKLRETCRAEY
                     TGVVLYQTGSLQVTEGGPVVAYGRIVREPEYDDRKEQIVHYWEKRSESFLEQRRSELA
                     NPIAKRWMKEIEKQIPAGRRLKILDVGCGAGFFSILLAKEGHEVFGIDLTPEMIENAI
                     QLAEEENADCCFQVMDAENPMFADETFDVVISRNLTWTLPNAEHAYGEWMRVLKTGGV
                     LLNFDANYGKEDVADTKGLPEAHAHFKVGNEMLEECERIKSQLPISRKNRPAYDVAVL
                     CENTAGEIRIDTSLGKRIYLEKDEFYNPAPMFSICAVKQ"
     gene            961..1902
                     /locus_tag="PLN77_06290"
     CDS             961..1902
                     /locus_tag="PLN77_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43921.1"
                     /translation="MKKYLLKRLLRLIPVFLGVTILAFLLTGLSSVDAVDLKESSSGG
                     VMSEAEKEAMREELGLNEPKAVQYLQWLKNALRGDFGESYVSGKPVLASFCEKLPATI
                     ALAALALAMTVCLSFPLGILSAKYQNTWADRSISLFCFAGNSFPGFFTALLLLYFFSL
                     KWNLFPVLSMGKGWKSCVLPAATLAVSMTAKYTRQVRSAVLAELEQEYVAGARARGLS
                     ENRILFGNVLKSVLLRMVTLLFLSAGSLLGGTAIVETIFMWNGVGKMAVDAVSMHDIP
                     VIQAYLVWMAAIYLLLHLIADLLMGALDPRAKLEGMR"
     gene            1899..2705
                     /locus_tag="PLN77_06295"
     CDS             1899..2705
                     /locus_tag="PLN77_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM43922.1"
                     /translation="MKKEQKRKILFYGILAGALLLFAACAAHLAPYDPYEQNLELSLL
                     PPSAAHWMGTDRYGRDLFSRILSGARVSIFSALLVVAASALIGTLAGIFSGWLEGKTD
                     TVLMQISDIFLAFPGMVFAIAFSGVLQSGMTGAVVSLILVSWPKYARLARNRVLALKH
                     MPFIAAARLTGNGTWKMIGRHILPNLAGQTIVTAVLDIGTMMLEIAGLSFLGLGAEAP
                     SAEWGAMMSNGRSMLQTAPWVILAPGLAIFLTVLVCNLLGEAVRKTVDQG"
     gene            2742..3578
                     /locus_tag="PLN77_06300"
     CDS             2742..3578
                     /locus_tag="PLN77_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM43923.1"
                     /translation="MSEKLLEISGLSVCYEGEQILNNICFSVEKGETVGIAGESGSGK
                     STLLRAILRLMGKNGSITGGQITFRGEELTGMSEKKLRSLCGSEIGMIFQNTGASLCP
                     TRKIGAQICESVRAHEKRCGFITPISGGMSGRKSRREIKDMTLQRFADMGLSDGETIW
                     NSYPSSLSGGMNQRVGIALAMLLHPSLLLADEPTSALDVVSQAQVLRELQALQQKEQT
                     GMVLVTHNLAVLEQMADRVAVLKDGNIVEQGETRTVFDHPKDEYTKNLLAAVPGRGKN
                     DA"
     gene            3571..4338
                     /locus_tag="PLN77_06305"
     CDS             3571..4338
                     /locus_tag="PLN77_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HRM43924.1"
                     /translation="MHNHNMLEVNNLSKNFARTGRETEFAAVDGISFTLDAGECLGLI
                     GESGCGKSTTARLITGLVQADAGSVLLEGEEILGRKGRRQREVYRKIQMVFQTPQDSF
                     DPMQTLETGILEAFRNQGMSRKEACLHLPELLKKVELSSETAMRYPRETSGGECQRAA
                     IARALAVQPSLLICDEATSALDAAVQAQIVQLLKKLQREEGLAMLWIGHDLALVRELC
                     SRVIVMRQGKIVEQGETREVLEYPKEEYTKLLMEYSL"
     gene            4410..5972
                     /locus_tag="PLN77_06310"
     CDS             4410..5972
                     /locus_tag="PLN77_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM43925.1"
                     /translation="MQRKNGRRKKIAYVLCGILAVVLFSGCQSTAADGTDEKVFVYGD
                     TTFNAENDESDVNPHNGYSGWACIRYGIGETLFHYSDSMEIEPWLAESYEIVDDTTWR
                     ITLHEGISFTSGRTLDAEAVKECLEDLIAVHDRARGDLKIESIEADGLIVTIHTEQPV
                     PALLNYLSDPYGCIIDMQAGVTDDGNVAGTGPYRAVQVETDQGLTLMKNDNYWNGTPK
                     LDRIEVKTIRDGDTMTMALQSGELDAAYGLPYSNLILFRDEPYTISSADTSRTFFGQF
                     NGSSGVLQDDRVREAVIGAIDREGFVNTLMEGNGSVAEGPFPSYFAFGDSKVQEESYD
                     LEYSRELLAEAGWTDEDGDGYVEKDGKRLTICWLTYPSRQELPLLAESAQASLKKIGI
                     EVQIQCTANHLELLKKGNWDVYVSAFVSAPTGDPEYFFTTHCLQDSSKNRGGYYSETL
                     EMLEQQLSGEFDTEKRTELAIEMTQTLLDDHMFFFASHLKMSMVSGEGVTGLTAHPCD
                     YYEITVNLDKNK"
     gene            6080..7489
                     /locus_tag="PLN77_06315"
     CDS             6080..7489
                     /locus_tag="PLN77_06315"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HRM43926.1"
                     /translation="MRKEELRTYIENHESEMIEDLKSLIRIPSEKQAAEPGKPFGAPA
                     AEALAQGIEILEKYGFAVTNYDNYAIAADFNQEEKGLDILAHLDVVPAGEGWQETDPF
                     TPLIKEGKMFGRGTADDKGPAIAAIYAMRAVKELGYPLKKNVRLVLGSDEECGSSDLE
                     YYYKKEVEAPCTFSPDAEFPVINIEKGGMRGHFEKTSAAGTTGVRLVSLQAGTKLNVV
                     PGKAYAELAGMAKEDLKTCADETEQKTGVSFVLEDTENGNIKITACGAFAHASTPEMG
                     NNALTALLQLLASVGMEESELKNDISFLAKVYPHGDGKGAGLGVAMSDEESGELTLSL
                     DLMNYDGKTLTGSYDCRAPICATKENLYDTAGQNMKKGGFSMEGSGMFPPHHVPADSP
                     FVQTLLQCYTDVTGKPGKPFAIGGGTYVHHLKNGVAFGCADLEVDNRMHGADEFVEIE
                     CLKECVVIFAEAILRICGE"
     gene            7595..>8302
                     /locus_tag="PLN77_06320"
     CDS             7595..>8302
                     /locus_tag="PLN77_06320"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRM43927.1"
                     /translation="MRETLGVLLGFFQAAVLGYLISEVAQKEQEKEVLESYMDSLQEI
                     YEVIRQRMDVTRKFRHDLLSHVQTLEYLIEKQKNPDGELRKYADQLTETYRKIQKGTF
                     CRDEITDAFLMMEKQVCIEKEIPFQIQVSKTCMEWMMPVERIGFLHNLLDNAIEANGR
                     IPEKERKELSFWIEEDSDSCTVKLRNALASKEVFNLSTWKTDGAEHGVGTKIIRAIVE
                     KYHGTSKTFWDKEKHRLE"
BASE COUNT         2173 a         1936 c         2459 g         1734 t
ORIGIN      
        1 gctatcaggc ggcttcggaa agcggcctgt ggtatccggc ggcgaaaccg ggcgatatgg
       61 ttgcagaggg cgcactgctc ggaacgatcc gtgattataa tggaaaactc cgtgagacgt
      121 gccgtgccga gtacaccggt gtagtgctgt accagacggg cagtctgcag gtaacagaag
      181 ggggccctgt cgttgcttac ggaagaattg tgcgcgagcc ggagtacgac gaccgcaaag
      241 agcagattgt ccattactgg gagaaacgga gcgagagctt tttagagcag agacgctccg
      301 aacttgcaaa cccgattgcg aagcgctgga tgaaagagat tgaaaaacag atcccggcgg
      361 gaagacggtt aaagatcctt gatgtgggct gcggagccgg atttttctcg atcctgctgg
      421 caaaagaggg ccacgaggtc ttcggcatcg acctgacacc ggagatgatt gagaatgcaa
      481 tccagcttgc cgaagaagag aatgccgact gctgcttcca ggtcatggac gcggaaaatc
      541 cgatgtttgc ggatgaaacg ttcgatgttg tcatttcccg aaacctgacg tggacgctgc
      601 cgaatgcgga gcatgcttac ggcgagtgga tgcgtgtgct gaaaacgggc ggtgtgctgc
      661 tgaacttcga tgccaattac ggaaaagaag atgttgccga tacgaaggga cttccggagg
      721 cgcatgccca tttcaaggtt ggaaacgaga tgctcgaaga gtgcgaacgg atcaaatcac
      781 agctcccgat cagccgcaaa aaccggccgg cgtacgatgt ggcggtgctg tgcgaaaaca
      841 cagcgggaga aatccgcatt gatacgagcc ttggaaaacg catttatctg gaaaaagacg
      901 agttctataa cccggcaccg atgttttcga tttgtgccgt gaagcagtaa aggagtattt
      961 gtgaaaaaat atctgttaaa acgactgctt cgcctgattc cggtattcct cggcgtgacg
     1021 attctcgctt ttctgctgac gggactttcc tcggtcgatg cggtggattt aaaagagagc
     1081 agcagcggcg gcgtgatgtc ggaggcggaa aaagaagcga tgcgggagga gctgggactg
     1141 aatgagccga aagctgtcca gtacctgcaa tggctgaaaa acgccctgcg cggtgacttc
     1201 ggggagtcgt acgtttccgg caaaccggtg cttgcaagct tctgcgaaaa actgcccgca
     1261 acgattgcac ttgcggcgct ggcgcttgcc atgacggtct gtctttcgtt tccgctcggt
     1321 atcttaagtg caaaatatca gaatacgtgg gcggaccgca gtatcagcct gttctgcttt
     1381 gcaggtaatt cgtttccggg gtttttcact gcacttcttc tcttgtattt cttttcgctg
     1441 aaatggaatc tttttccggt tctcagtatg ggaaagggat ggaaaagctg tgtgcttccg
     1501 gcggcgacgc ttgcggtttc gatgacggca aaatacaccc gtcaggtgcg ttctgccgtg
     1561 cttgcagagc tggagcagga gtacgtggcg ggtgcacggg cacgtgggtt aagtgaaaac
     1621 cggattctgt ttggaaatgt attaaaatcc gtgctgcttc ggatggtcac gctgctattc
     1681 ttatccgccg gttcgctgct tggaggaacc gccatcgttg agacgatttt catgtggaat
     1741 ggtgtcggaa aaatggccgt ggatgccgtt tccatgcacg atattcccgt gattcaggca
     1801 tatctggtgt ggatggcggc gatctatctg ctgctgcatc tcattgctga tctgctgatg
     1861 ggtgcactgg acccgcgggc gaagctggag ggaatgcgat gaaaaaagaa cagaaaagaa
     1921 agatcctttt ttatggcatc ctcgccggtg cgctgctgct gtttgccgcg tgtgcggccc
     1981 atctggcacc gtacgatccg tatgagcaga atctggaact gtcccttctt ccgccgtccg
     2041 cggcgcactg gatggggacg gaccgctatg gaagagatct gttttcaagg attctctccg
     2101 gcgcgcgggt cagcattttt tcagcacttc ttgtcgttgc ggcaagcgct ctgatcggaa
     2161 cactcgccgg aattttcagc ggctggctgg aaggtaaaac ggatacggtt ctgatgcaga
     2221 tctccgatat ttttctggcc tttccgggta tggtatttgc aatcgcattt tccggcgttc
     2281 tgcagagcgg catgacggga gccgttgtgt cgctgatcct tgtttcctgg ccaaaatatg
     2341 cacggcttgc acggaaccgt gtgctggcat tgaaacacat gccgttcatc gcggcggcgc
     2401 gccttacagg aaacggaaca tggaagatga tcggacgcca tattctgccg aatcttgcgg
     2461 ggcagacgat tgtcacagcg gtgcttgata tcggaacgat gatgctggaa atcgcgggtc
     2521 tgtccttcct cggccttggt gcggaagcgc cgtcggcgga gtggggagcg atgatgagca
     2581 acggaagaag tatgctgcag accgcgccct gggtcattct ggcgccggga ctggcgattt
     2641 ttctgaccgt tcttgtctgc aatctgcttg gtgaggcggt acggaaaacg gtggatcagg
     2701 gataaggggc aaacgaaaat ggaaacaaag ggagcaaaaa catgagcgaa aaactgctgg
     2761 aaatcagcgg cctttctgtc tgttatgagg gggagcaaat tttaaataac atctgttttt
     2821 ccgtcgaaaa aggagaaacc gtcggcatcg ccggggagtc gggcagtgga aaaagtacct
     2881 tgctgcgagc gatcctgcgg ctgatgggaa agaatggctc catcaccggc gggcagatca
     2941 catttcgcgg agaggagctg acgggcatgt ccgaaaagaa gctgcggagt ctgtgcggtt
     3001 cagaaattgg tatgattttc cagaatacag gggcatcact ctgtccgacc cggaaaatcg
     3061 gagcacagat ctgtgaaagc gtgcgcgccc atgagaaacg ctgcggattc atcaccccga
     3121 tttctggggg aatgtctggc agaaaaagcc gcagagaaat caaagatatg acgctccagc
     3181 gatttgcgga tatggggctt tcggatggcg agacgatctg gaacagctat ccatcgtcgc
     3241 tctccggcgg catgaaccag cgcgtcggga ttgcgctcgc aatgctgctg catccgtcgc
     3301 ttcttctggc ggacgagccg acgagtgcac tcgatgtggt ttcgcaggcg caggtgctgc
     3361 gggagcttca ggcgttgcag cagaaagagc agacggggat ggtgctggtg acgcacaatc
     3421 ttgcggttct cgagcagatg gcagaccggg ttgctgtttt gaaggatgga aacatcgtgg
     3481 agcaggggga gacaaggacg gttttcgatc acccaaagga cgaatataca aaaaatctcc
     3541 ttgcggctgt gccgggaaga gggaagaacg atgcataatc ataacatgct ggaagtaaac
     3601 aacttaagca aaaactttgc tagaaccggc cgggaaacgg aatttgccgc cgtggacggc
     3661 atcagcttca cactggatgc gggggaatgt cttggcctga tcggggagag cggatgcgga
     3721 aaaagcacga cggcccgtct gatcaccgga ttggtccagg cggatgcagg cagcgttctg
     3781 ttggaaggcg aggaaatcct cggaagaaaa ggcaggcggc agcgcgaagt ttaccggaaa
     3841 atacagatgg tcttccagac cccgcaggat tccttcgatc caatgcagac gctcgaaacc
     3901 gggattctgg aagcttttcg gaatcaggga atgagccgga aagaggcgtg tcttcatctg
     3961 ccggagctgc tgaaaaaagt ggaacttagt tcggaaacgg cgatgcgcta tccccgcgag
     4021 acgagcggcg gagagtgcca gagagccgca attgcgcggg ctcttgctgt gcagccgtct
     4081 cttctgatct gcgacgaggc aacgagtgcg ctcgatgcgg cggtacaggc gcagatcgtt
     4141 caactgctga aaaaactgca gcgggaagaa ggacttgcga tgctgtggat cggccacgac
     4201 cttgcgctgg tgcgggaact ctgcagccgt gtgattgtca tgcggcaggg aaaaatcgtg
     4261 gagcaggggg agacgcggga ggtattggag tacccgaaag aggaatacac gaaactgttg
     4321 atggagtata gtctgtgaga aaactgttga aacagaaaat cagacagcag aaatggtggt
     4381 gccatttgcg aaaacaggag aagtacagaa tgcagagaaa gaacggcaga aggaaaaaaa
     4441 ttgcatatgt tttgtgcggc attctggcgg tggtactgtt ttccggttgt cagagcacag
     4501 cggcagatgg tacagatgaa aaagttttcg tatacggcga cacgacattc aacgccgaaa
     4561 acgacgagtc cgatgtcaac ccgcacaacg gctattccgg ctgggcgtgt atccgctatg
     4621 ggatcggtga gacgctgttt cattattccg attctatgga gatcgagcca tggctggcag
     4681 aaagctatga gattgtggac gatacaacat ggcggattac actgcatgaa ggcatctcct
     4741 ttacgagtgg gcgcactctc gacgcggagg cggtcaaaga atgtctcgag gatctgatcg
     4801 cagtacatga ccgtgcacgc ggggatctga aaattgagag catcgaggca gacggcctga
     4861 tcgtaacgat tcacaccgaa cagcctgtcc cggcgcttct gaattatctg tctgacccat
     4921 atggatgcat cattgatatg caggcaggtg tgacggatga cggcaatgtc gccggaaccg
     4981 gcccgtaccg cgcagtacag gtggagacgg atcagggact gacgctcatg aaaaatgaca
     5041 attactggaa cggaacgcca aagctcgacc ggatcgaagt caaaacaatc cgcgacggcg
     5101 atacgatgac gatggcgtta cagtcaggcg agctcgacgc ggcgtacggc ctgccgtatt
     5161 ccaatctgat cctgttccgc gatgaaccat acacgatttc ttcggcggat acgtcgcgta
     5221 ccttcttcgg acagttcaac ggatcatccg gggtgctgca ggacgaccgc gtgcgcgagg
     5281 cagtcatcgg agcgatcgac cgggagggct ttgtgaatac gctgatggaa ggaaacggaa
     5341 gcgtggcgga aggcccgttc ccctcttact ttgcattcgg cgacagcaaa gtgcaggagg
     5401 agtcctatga tcttgagtat tccagagaac tgctggctga agcaggctgg accgatgagg
     5461 acggcgacgg ctacgtggaa aaagacggaa aacgcctgac catctgctgg ctcacgtatc
     5521 cgagccgcca ggaactgccg cttctcgcgg agagtgcaca ggcatcgctg aagaaaatcg
     5581 gaatcgaagt acagatccag tgcacggcaa atcatctgga actgctgaaa aaaggaaact
     5641 gggatgttta tgtcagcgca tttgtcagcg caccgacggg cgatcccgag tatttcttta
     5701 caacgcactg cctgcaggat tcctccaaga accgcggcgg ttattacagt gagacactcg
     5761 agatgctgga gcagcagtta agcggcgagt ttgataccga aaaacgaacc gaactggcca
     5821 tcgagatgac gcagacgctg ctggatgacc acatgttttt cttcgcatca cacctcaaaa
     5881 tgtcgatggt atccggcgag ggcgtgacgg ggcttacggc gcatccgtgc gattattatg
     5941 aaattacagt caatttggac aaaaacaaat gattatttgg caaaagagca gaaaatgtgg
     6001 attttctgct ctttctgcgc tatgatagga aaagaacggc actgcgacgg tgccagagaa
     6061 gagaaaagga gaagttacga tgcggaaaga agaattaaga acatatattg aaaaccacga
     6121 atccgaaatg atcgaggatc tgaaaagcct gatccggatt ccaagcgaaa aacaggcggc
     6181 agagccaggc aaaccattcg gcgcaccggc ggcagaagca ctggcgcagg gaatcgaaat
     6241 tctggaaaaa tacggctttg ccgtaaccaa ctacgacaac tacgcgattg cagctgactt
     6301 taatcaggaa gaaaagggcc ttgatattct cgcccatctc gatgtcgtac cggcaggcga
     6361 aggctggcag gagaccgatc cattcacccc gctcatcaaa gaaggaaaaa tgttcggccg
     6421 cggaaccgca gacgacaaag gaccggcgat cgcagctatc tacgcaatgc gcgctgtcaa
     6481 agaactcggt tacccattaa agaaaaacgt ccgtcttgtc ctcggttccg acgaggagtg
     6541 cggaagctct gacctcgaat attattataa gaaagaagtg gaagcaccat gcaccttctc
     6601 accggatgcc gaattcccgg tcatcaacat tgaaaaaggc ggcatgcgcg gccacttcga
     6661 aaaaacatcc gctgccggaa cgacaggcgt gcgcctcgta tccttacagg ccggcaccaa
     6721 actgaacgtt gtaccaggca aagcatatgc agagcttgcc ggcatggcaa aagaagacct
     6781 gaaaacctgc gctgacgaga ctgagcagaa gaccggcgtt tccttcgttc tggaagacac
     6841 cgaaaacggc aatataaaaa tcaccgcctg cggtgccttc gcccacgcat ccaccccgga
     6901 aatgggtaac aacgcactga ccgcactgct gcagcttctc gcatccgttg gaatggaaga
     6961 gagtgaactg aaaaacgaca tcagtttcct tgcaaaagtt tacccgcacg gcgacggcaa
     7021 aggcgcaggc ctcggtgtcg ccatgtcaga tgaagagtcc ggcgagctca ccttaagcct
     7081 cgacctcatg aactacgacg gcaaaacctt gaccggttcc tacgactgcc gcgccccgat
     7141 ctgcgcgaca aaagaaaacc tgtacgatac cgccgggcag aacatgaaaa aaggcggctt
     7201 ctccatggaa ggcagcggaa tgttcccacc acaccacgtc ccggccgaca gcccgtttgt
     7261 ccagaccctg ctgcagtgct acacggatgt cactggaaaa ccaggcaaac catttgcaat
     7321 cggcggcgga acgtatgtcc atcacctgaa aaacggcgtt gctttcggct gtgcggacct
     7381 tgaagtcgac aaccgtatgc acggagcaga cgagttcgtg gaaatcgaat gcctgaaaga
     7441 atgcgttgtg atctttgcag aggcgattct gcggatctgc ggggaataaa aatcccgtca
     7501 gtacaagatg aaagcagagt ccttatgctg tgaaccattt tgagcggtgt gggactctct
     7561 taccaaagaa gaaggaatag atgagggcgg acaaatgagg gaaacactgg gagttttgct
     7621 gggatttttt caggcagccg tacttggcta tctgatttca gaagtcgcgc agaaggaaca
     7681 ggagaaagaa gttctggaaa gctatatgga ttcccttcag gagatttatg aagtgatccg
     7741 gcagagaatg gatgtgacga gaaaattccg tcatgatctt ctgagccatg tgcagacgct
     7801 ggagtatctg atagaaaagc agaaaaatcc ggatggggag cttcgaaaat atgcggatca
     7861 gctgacagaa acatatcgga aaattcagaa aggaaccttc tgccgggatg agatcacaga
     7921 tgcatttctg atgatggaga aacaggtctg tatagaaaaa gagattccct ttcagatcca
     7981 ggtatcgaaa acctgcatgg aatggatgat gcccgtagaa cgaatcggtt ttttacataa
     8041 tttattggat aatgcaatcg aagcgaacgg aagaattccg gaaaaggaaa gaaaagagct
     8101 ttctttttgg atagaagaag attccgattc ctgtacggtg aaattgagaa atgcgcttgc
     8161 aagcaaagag gttttcaact tatcaacctg gaaaacagat ggagcagaac acggcgtggg
     8221 aactaaaatc attcgtgcca ttgttgaaaa ataccacgga accagcaaaa ccttctggga
     8281 taaagaaaaa caccgcctgg ag
//
LOCUS       DAOMUF010000088         8294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2231_length_8294_cov_3.001578, whole genome shotgun sequence.
ACCESSION   DAOMUF010000088 DAOMUF010000000
VERSION     DAOMUF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2231_length_8294_cov_3.001578"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLN77_06325"
     CDS             <1..1018
                     /locus_tag="PLN77_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRM43928.1"
                     /translation="TCGGGMALIPAMTDFTFMESKNGKLFVNSPNALDGNHVSKCDTA
                     SADFQGEEAGLVDFAGTEEEILGQIRNLVSMLPANNEDEAYTECEDDLNRACADLANC
                     AGDTGILLSQLSDNGIYFETKAAYGKDVVTAFIQLNGATVGAVANRSEIYGEDGTVKE
                     KMDGTLSARGAKKAADFVNFCDAFCIPVLTITNVTGFKATKCSERTMAKNAAALTAAF
                     ANATVPKVNVVVGKAYGSAYVVMNSKSIGADMVFAWPNAEIGMMDAKSAAKIMYEGSD
                     AATINEKAAEYATLQNNVTSAARRGYVDTIIEPEDTRKYVIGAFEMLYTKREDRPDRK
                     HGTV"
     gene            1034..1807
                     /locus_tag="PLN77_06330"
     CDS             1034..1807
                     /locus_tag="PLN77_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HRM43929.1"
                     /translation="MRKNWKKLVSLVLTAACTMVVTVAPVFADAASTPAYSPVEASTV
                     DSLLSSSAQWVGQSLMTYSDDDLANMEASDQQFYARAAEAWEEAKADLGEVKSLEDGT
                     LEVDGDFVTCTIPGEFEKNPGIVRVFWNISTGTPVPTDLTITVEESKGVLIGQAGMNT
                     IIGIGTVFVMLIFLIFVISSFDYILGDKKKKKAPAAPVQEAPKAVAAPVVEETAPEAD
                     DMELQAVITAAIAMYEEDMASASGDAYVARPIRRKGKRA"
     gene            1822..2238
                     /locus_tag="PLN77_06335"
     CDS             1822..2238
                     /locus_tag="PLN77_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRM43930.1"
                     /translation="MKNYTITVNGNVYNVTVEEGTTAGAAPTVSAAKAAPAAKAAPAA
                     PKAAAPGAAPKAAPKAAAPAGAAGSVEVTASVPGKICKIEANVGTAVKAGDTVLVLEA
                     MKMEIPVVAPQDGTVASVNVAVGDAVESGDVLATMN"
     gene            2272..3351
                     /locus_tag="PLN77_06340"
     CDS             2272..3351
                     /locus_tag="PLN77_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004052667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRM43931.1"
                     /translation="MITETLNNLLHQTAFFNLDWGNYVMIAVACFFLYLAIKHEFEPL
                     LLVPIAFGMLLVNIYPDIMAEPYTDVQGLEHAGGLFYYFFTLDEWSILPSLIFMGVGA
                     MTDFGPLIANPISFIMGAAAQLGIYLAYFFAIFLLNKLGQQHLMGPIAVAAYSYMSLV
                     PIIQPPVMKLLTTEKERKIKMEQLRSVSKLEKILFPIVITIVVCMILPSTAPLVGMLM
                     LGNLFRECGVVKQLQETASNAMMYIVVILLGTSVGASTSAEAFLNVSTLKIVALGLIA
                     FVFGTAGGVLLGKVLCKATHGRINPLIGSAGVSAVPMAARVSQKVGAEADPTNFLLMH
                     AMGPNVAGVIGTAVAAGTFMAIFGV"
     gene            3401..4810
                     /locus_tag="PLN77_06345"
     CDS             3401..4810
                     /locus_tag="PLN77_06345"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HRM43932.1"
                     /translation="MAVEKKPVKIMETVLRDAHQSLIATRMTTEQMLPIIDKMDKVGY
                     HAVECWGGATFDASLRFLHEDPWMRLRKLRDGFKNTKLQMLFRGQNILGYRPYADDVV
                     EYFVQKSIANGIDIIRIFDCMNDLRNLQTAVSAANKEKGHAQVALSYTLGDAYTLDYW
                     TTMAKRIEEMGADSICIKDMAGLLVPYKATELVTALKEATDLPIQLHTHYTSGVASMT
                     YLKAVEAGVDIIDTAMSPFAMGTSQPATEVMVETFKGTPYDTGLDQNKLAEIADYFRP
                     MRDEALDTGLLNPKNLGVNIKTLLYQVPGGMLSNLTSQLKEQHAEDKYYDVLEEVPRV
                     RKDLGECPLVTPSSQIVGTQAVFNVLMGERYKMVTKETKDVLAGKYGATVKPFNPEVQ
                     KKCIGDTEPITCRYADLLEPELPKLEKEIEQWKQQDEDVLTYALFPQVAVDFFKYREA
                     QQTKVDPAAADKKDGAYPV"
     gene            5040..6014
                     /locus_tag="PLN77_06350"
     CDS             5040..6014
                     /locus_tag="PLN77_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM43933.1"
                     /translation="MKKEKEQSSSEEQKAANENLLIRLNQNYGKLSKGQKRLADFITA
                     HYDQAVSMTAARLGQQVGVSESTTVRFAMQLGYAGYPEFQRALEEMVMNRLNSVQRMQ
                     FTYGRVPQGEILETVLQSDIEKIKMTLEEVDRSAFERAADTILSARTIYIVGIRSCAP
                     LASFLAFYLHMIFPDVRQVQTNSSSEIFEQMIRITEDDVVIGISFPRYSMRTMKALEF
                     ANSRKAKVITITDSVHSPMNVYAACTLIAKSDMASIVDSLVAPLSVINALVVALCMKR
                     QADVKKTLESMEKLWDEYQVYSNDEINGLDDARPMGGKKAALPVKEKA"
     gene            6011..7258
                     /locus_tag="PLN77_06355"
     CDS             6011..7258
                     /locus_tag="PLN77_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM43934.1"
                     /translation="MRKAAVIGGGAAGMMTAVLAAGEKTEIHLYEKNEKLGKKLFITG
                     KGRCNVTNDCPTEEFFTNVVTNPKFLYSSAYSFDSAQVQEFFEAAGVRLKVERGNRVF
                     PESDHSSDIIRALETELKRKNVQVHKNTKVKELLFEGDRVKGIRLENGKELFYDDVVV
                     ATGGMSYQTTGSDGDGYRFAEEAGLAVTPLRPALVPLETEEAYIRELQGLSLKNVTMT
                     IKNGKKTLFDGFGEMLFTHFGISGPLGLSASSYIGKALEQQPLKGYLNLKPALTEEQL
                     DARILREFEENRNKQFRNVINSLFPAKLLPVMLSLGGIDPYKQVNAVSKAERQHFEEL
                     ITHFPFTVTGTRGFREAIITQGGVSVKEIRPGTMEAKKKEGLYFVGEVLDLDALTGGF
                     NLQIAWASAHAAAEEIRAKEEQE"
     gene            7303..7968
                     /gene="cmk"
                     /locus_tag="PLN77_06360"
     CDS             7303..7968
                     /gene="cmk"
                     /locus_tag="PLN77_06360"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRM43935.1"
                     /translation="MRFNLAIDGPAGAGKSTIAKRVAKELSFVYVDTGAMYRAMGIYF
                     SDLGIAPEEQEKIEAACKDVKISISYENGEQQVYLNGVNVTGRLRTEEAGKMASATSA
                     YLEVRKKLVELQQEMAENTDLVMDGRDIGTAVLPNAPLKVYLTASAHVRALRRWKELT
                     EKGQTADLAKIEEDINERDYQDMHREHSPLVQAEDAVLVDSSEMTIDEVVEKILSLAR
                     ERM"
     gene            8001..>8294
                     /locus_tag="PLN77_06365"
     CDS             8001..>8294
                     /locus_tag="PLN77_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRM43936.1"
                     /translation="MEVTVAKSAGFCFGVKRAVDTVYREIESGEKPVYTFGPIIHNEQ
                     VVEDLENRGVQVIHSEDELEGLSGGTVVIRSHGVSRDVCEKIEARGMKIVDATC"
BASE COUNT         2355 a         1944 c         2229 g         1766 t
ORIGIN      
        1 cacatgcggc ggcggtatgg cactcatccc ggctatgaca gacttcacgt tcatggagtc
       61 taaaaacgga aaacttttcg taaactcccc gaacgcactg gacggaaacc acgtatccaa
      121 atgtgatacc gcatccgcag atttccaggg cgaagaagca ggtcttgttg actttgctgg
      181 aaccgaggaa gaaatcctcg gacagatcag aaacctggta tctatgcttc cggcaaacaa
      241 cgaggacgaa gcatacaccg agtgcgagga tgatctgaac cgtgcatgtg cagaccttgc
      301 aaactgcgca ggcgataccg gaatccttct ttcccagctc agcgacaacg gcatctattt
      361 tgagaccaaa gccgcttacg gaaaagatgt tgtaaccgca ttcatccagt taaacggagc
      421 aaccgtagga gcagttgcaa accgctctga aatctacggc gaggacggaa ctgtaaaaga
      481 gaaaatggat ggaaccttaa gcgcaagagg agccaaaaaa gcagcagatt tcgtaaactt
      541 ctgcgatgct ttctgcatcc cggtacttac catcacaaac gtaaccggat tcaaggcaac
      601 caaatgctct gagagaacca tggctaagaa cgcagcagcc ctgaccgcag ctttcgcaaa
      661 cgcaaccgtt ccgaaagtaa acgttgttgt cggcaaagca tacggaagcg catacgttgt
      721 catgaacagc aaatccatcg gagcagacat ggtatttgca tggccgaacg cagaaatcgg
      781 aatgatggat gcaaaatctg cagcaaagat catgtacgaa ggatctgacg ctgctaccat
      841 caacgagaaa gcagctgaat atgcaaccct tcagaacaat gtaacttccg cagccagaag
      901 aggctatgtg gacaccatca tcgagccgga agacaccaga aaatatgtta tcggcgcatt
      961 cgagatgctg tataccaaga gagaagacag accggacaga aaacacggaa cagtctaagg
     1021 aaggtggcat acgatgagaa agaactggaa aaaattagta agtctcgtcc ttaccgcagc
     1081 ctgcaccatg gttgtgaccg tagctcctgt ttttgcggat gcggcatcta caccggctta
     1141 ttcaccggtt gaggcgtcta ctgtagattc tctgctctcc tccagtgctc agtgggtagg
     1201 acagagcctg atgacctaca gcgatgacga tcttgccaat atggaagctt ccgaccagca
     1261 gttctatgca agagcagctg aggcatggga agaggcaaag gctgacctcg gagaggtaaa
     1321 aagcctggaa gacggtactc tcgaagtaga cggagatttt gtaacctgca caattccggg
     1381 tgaatttgaa aaaaatcctg gtattgtccg cgtattctgg aacatcagca ccggaacacc
     1441 ggttccgacc gacctcacca tcaccgttga ggagtcaaag ggcgttctga tcggccaggc
     1501 cggcatgaac accatcatcg gaatcggtac cgtatttgtt atgctgattt tcctgatttt
     1561 cgttatttct tcgtttgatt acattttggg agacaagaag aagaaaaaag caccggcagc
     1621 acctgtacag gaagcaccga aggctgtcgc agcaccggtt gttgaagaga cagcaccgga
     1681 agcagacgat atggagctgc aggctgttat tacagctgca atcgccatgt atgaagagga
     1741 tatggcttct gcatccggag atgcttatgt tgcacgtccg atccgcagaa aaggcaaaag
     1801 agcttaagga ggaaattttc aatgaaaaac tacaccatta ccgtaaacgg aaatgtatat
     1861 aacgtaactg tagaagaagg aaccacagca ggcgcagctc caacagtcag cgcagcaaaa
     1921 gcagctccgg cagcaaaagc agctccggca gcaccaaaag cagcagcacc aggggcagcg
     1981 cccaaggcag ctcccaaagc agcagctccg gcaggtgcag caggcagcgt agaggttact
     2041 gcttctgtac ctggtaaaat ctgcaaaatc gaagcaaacg taggcacagc tgtaaaagca
     2101 ggcgatacgg ttcttgtact ggaagccatg aagatggaaa tcccggttgt agcaccgcag
     2161 gacggcacag tagccagcgt aaacgtagca gtaggcgatg cagtagaatc cggtgacgtt
     2221 cttgcaacca tgaactaatg cccgttgtaa gaaaaaaccg gaggtgaaaa aatgattact
     2281 gaaactttaa acaacctgct tcaccagacg gcgtttttta atctggactg gggcaattac
     2341 gttatgatcg cagtggcttg cttcttcctg tatcttgcga ttaagcatga atttgagccg
     2401 ttgcttcttg taccgatcgc attcggtatg ctgcttgtaa acatttaccc ggacatcatg
     2461 gctgagcctt ataccgacgt acagggtctt gagcatgcgg gcggtctgtt ctactatttc
     2521 tttactctgg acgagtggag tatcctccca tccctgatct tcatgggtgt aggtgcgatg
     2581 acagacttcg gtccgctgat tgcaaacccg atcagcttca tcatgggagc tgcagcacag
     2641 cttggtatct atctggcata cttcttcgct atcttccttc tgaataagct cggacagcag
     2701 cacctgatgg gaccgattgc cgtagcagca tattcctata tgtcactggt gccgatcatc
     2761 cagccgccgg tcatgaagct tctgactaca gagaaagagc gtaaaatcaa aatggagcag
     2821 cttcgttccg tatccaaact cgagaagatc ctgttcccga ttgttatcac catcgttgtc
     2881 tgcatgatcc ttccatctac cgctccgctg gtaggtatgc tgatgcttgg taacctgttc
     2941 cgtgagtgcg gcgttgtaaa acagctccag gagacagctt ccaatgccat gatgtacatc
     3001 gtagttatcc ttcttggtac ttccgttggt gcatccacaa gtgctgaggc attcttaaat
     3061 gtcagcacct taaagatcgt tgccctgggt ctgatcgcat tcgtattcgg caccgcaggc
     3121 ggtgtcctgt taggaaaggt actgtgcaag gcaacccacg gaagaatcaa cccgctgatc
     3181 ggttctgcag gtgtatctgc ggtacctatg gcagcccgtg tatcccagaa agtcggtgcg
     3241 gaggcagatc cgacgaactt cctgctgatg catgcaatgg gaccgaatgt agccggtgtt
     3301 atcggtacag ccgtagcggc cggtaccttc atggcgatct tcggtgtata acttcttaag
     3361 acaaacaaaa gtgagaaaca cttaatttag gaggatagaa atggcagtag aaaagaaacc
     3421 tgtcaagatt atggaaactg ttctgcgtga tgcacatcag tccctgatcg cgacgagaat
     3481 gacaacagaa cagatgcttc cgatcatcga taaaatggat aaagtcggat atcacgcagt
     3541 agagtgctgg ggaggtgcaa ctttcgatgc ttccttacgt tttctgcatg aagatccatg
     3601 gatgagactg agaaaactcc gtgacggatt caaaaataca aaactgcaga tgctgttccg
     3661 tggacagaat atcctgggat atcgtccgta cgcagacgac gtagtagagt atttcgtaca
     3721 gaaatccatt gcaaacggaa tcgacatcat ccgtattttt gactgtatga acgatctgcg
     3781 caacctgcag accgctgtca gcgcagcaaa caaagaaaaa ggccacgcac aggtagcact
     3841 ttcctatact ttaggagatg cttacaccct ggattactgg accaccatgg caaagagaat
     3901 cgaagagatg ggtgcagatt ccatctgtat taaagatatg gcaggcctgc tcgttccgta
     3961 caaggcaact gagctggtaa ccgcgctgaa agaagcaacc gatcttccga ttcagctgca
     4021 cacccactat acttccggtg ttgcatccat gacgtacctg aaagcagtag aggctggtgt
     4081 tgatatcatc gataccgcaa tgtctccatt cgctatgggt acttcacagc cggcaactga
     4141 ggttatggtt gaaaccttca aaggcactcc atacgatact ggtctggatc agaacaaact
     4201 ggctgagatc gcagattact tccgtccgat gagagacgaa gcgctcgata caggcctctt
     4261 gaatccgaag aaccttggcg taaatatcaa gactcttctg tatcaggtac cgggcggtat
     4321 gctctccaac ctgacaagcc agttaaaaga gcagcacgca gaggacaaat actacgatgt
     4381 actcgaagaa gtaccgcgcg taagaaaaga cctcggcgag tgcccgctgg ttaccccgtc
     4441 ttcccagatc gttggtaccc aggctgtatt caatgtcctg atgggcgagc gttataagat
     4501 ggtaacgaaa gagacaaaag acgttcttgc cggaaaatac ggtgcaactg taaaaccgtt
     4561 caatccggaa gtacagaaga aatgcatcgg tgataccgag ccgatcacct gccgctacgc
     4621 agacctgctt gagccggagc ttccgaaact ggagaaagag atcgagcagt ggaaacagca
     4681 ggatgaggac gtattaacct acgctctgtt cccgcaggta gctgtagact tcttcaaata
     4741 tcgtgaagca cagcagacaa aagtagatcc ggcagccgct gacaaaaaag acggcgcata
     4801 tccggtctaa ataaaatctg agtaaaaaat cagccggtgt tctgtgttcc ccgtaaaaag
     4861 ggaatgcaga acaccggctt tccttttcag cttttcagcc attgcgccag taatttattc
     4921 gtgttataat tcaaaagatc gaggtgtgtc cgaaaaagaa tcaggatgcc cccaaaagaa
     4981 gaaatgtgga tgccgaacgg catttgcaga tcggaaaaac aaatcaggaa gaaaataaca
     5041 tgaaaaaaga aaaagaacag tcttcttcag aagagcagaa ggcagcgaac gagaatctcc
     5101 tcattcgtct gaatcaaaat tacggaaagt tgagcaaagg acagaagagg ctggcagatt
     5161 ttattacggc acattatgac caggccgtat cgatgacggc agcgcgcctg ggacagcagg
     5221 tcggcgtcag cgaatcgaca acggttcgct ttgcgatgca gcttggctac gcgggatatc
     5281 cggagttcca gcgcgcactg gaggagatgg tgatgaaccg tctgaattcg gtgcagcgga
     5341 tgcagtttac gtatggccgc gtgccgcagg gagaaattct ggagaccgtc ctgcagtcgg
     5401 atattgaaaa aatcaagatg acgctggaag aagtggaccg gagcgccttt gagcgcgctg
     5461 cggatacgat tctttctgcg cggacaatct acattgtcgg cattcggagc tgcgcgccgc
     5521 ttgcgagctt tctggcgttc tatctccata tgattttccc ggatgtgcgc caggtacaga
     5581 caaacagttc cagcgagatt ttcgagcaga tgatccgcat cacggaggat gatgtggtga
     5641 tcggcatcag cttcccgcgg tattcgatgc gtacgatgaa ggcactggaa tttgcgaaca
     5701 gccgcaaagc gaaggtgatt acgatcacag acagtgtgca ttcaccgatg aatgtttatg
     5761 cagcctgtac gctgattgcc aagagcgata tggcatcgat cgtcgattct ctggtggccc
     5821 ctttgagtgt aatcaatgcg cttgtcgtgg cgctctgcat gaaacgccag gcggatgtga
     5881 aaaagacact tgagagcatg gaaaaactgt gggatgagta ccaggtttac agcaacgatg
     5941 aaatcaacgg gctggacgat gcacgtccca tgggcggaaa aaaggcagct ctgccggtaa
     6001 aggagaaagc atgagaaaag cagcagtaat cggcggaggc gccgccggaa tgatgaccgc
     6061 cgtgcttgcc gccggcgaaa agacagagat tcatctctat gaaaaaaatg aaaagctggg
     6121 aaagaagctg tttatcacag gaaagggccg ctgcaatgtg acaaacgact gcccgacaga
     6181 agagttcttc acgaatgtgg tgacaaaccc gaaatttctg tacagctccg cctacagctt
     6241 tgacagcgcc caggtgcagg agtttttcga agcggcaggt gtccgcttaa aagtggagcg
     6301 cggaaaccgt gtcttcccgg agtcagatca ctcctcggat attatccgcg cgctggagac
     6361 ggaactgaaa cggaaaaatg tgcaggtgca taagaataca aaagtaaaag agcttctttt
     6421 cgaaggagac cgcgtgaaag gaatccgcct ggaaaacggg aaagagcttt tttacgatga
     6481 tgtcgttgtc gcaaccggcg gtatgtcgta ccagacgacg gggtcggatg gagacggcta
     6541 ccgtttcgca gaagaggcag gacttgccgt cactccgctc cgcccggcgc tggtgccgct
     6601 ggaaacagag gaagcgtaca tccgtgagct gcagggactc tccctgaaaa atgtgacgat
     6661 gacgattaaa aacggcaaaa agacgctgtt cgatggattc ggcgagatgc tgtttacaca
     6721 tttcggcatt tccggcccgc tggggctttc tgcgagctcc tacatcggaa aagcactgga
     6781 acagcagccg ctgaaaggat atctgaacct gaagccggca ctcacggaag aacagctgga
     6841 tgcgcggatc ctgcgtgaat ttgaggaaaa ccggaacaaa cagttccgca acgtgatcaa
     6901 cagtctgttc ccggcgaaac tgcttccggt gatgctctct cttggcggga tcgacccgta
     6961 caaacaggtc aatgccgtat cgaaagcgga gcgtcagcac tttgaagagc tgatcacgca
     7021 tttcccgttt acagtaaccg gaacacgggg cttccgtgaa gcaattatca cacagggtgg
     7081 tgtttccgtg aaagagatcc gtccgggcac gatggaggca aagaaaaaag aaggtctgta
     7141 cttcgtagga gaagtgctcg atctcgatgc actgaccggc ggcttcaatc tgcagattgc
     7201 ctgggcaagt gcccacgctg cagccgaaga gatccgggca aaggaagaac aggaataaaa
     7261 tatggatcac agattttaga attttcagga ggaagagaga agatgagatt taatcttgca
     7321 attgacggac cggcaggcgc tggcaaaagt acaatcgcaa aacgtgtggc aaaggagctt
     7381 tcgtttgtct acgttgatac aggagcaatg taccgtgcca tgggcatcta tttttcagat
     7441 cttggcattg caccggaaga gcaggaaaaa attgaggcag cctgcaaaga tgtgaaaatt
     7501 tccatttcct acgaaaacgg agagcagcag gtttatttaa acggcgtcaa tgtcaccgga
     7561 agactgcgca ccgaggaggc tggaaagatg gcatccgcga caagcgccta tctggaagta
     7621 cggaaaaaac tcgtggagct gcagcaggag atggcggaga atacggatct tgtgatggac
     7681 ggccgcgata tcggaactgc cgttctgccg aacgcaccgt tgaaagtcta tctgacagca
     7741 agtgcccatg tccgcgcact gcgccgctgg aaagagctga ccgaaaaggg acagacagcc
     7801 gatcttgcga aaatcgagga agatatcaat gagcgtgatt atcaggacat gcacagagag
     7861 cattccccgc tggtacaggc agaagacgcc gtactcgttg acagctctga aatgacgatc
     7921 gatgaggttg tggaaaagat cttaagcctt gcccgcgagc gcatgtaaac gcggcagaga
     7981 aaagaggaga agaaaaacag atggaagtaa ctgttgcaaa aagtgcaggc ttctgcttcg
     8041 gcgtcaaacg ggcggtcgat acggtatacc gtgaaattga gagcggtgaa aagccggtct
     8101 acaccttcgg gccgattatt cacaacgaac aggttgtgga ggatctcgaa aaccgcggcg
     8161 tgcaggtgat ccattcggaa gatgaactag aggggctttc cggcggaacc gttgtgatca
     8221 ggtcgcatgg cgtgtcgcgg gatgtctgtg aaaagatcga agcgcgcggc atgaagattg
     8281 tagatgcgac gtgc
//
LOCUS       DAOMUF010000089         8218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2249_length_8218_cov_4.360529, whole genome shotgun sequence.
ACCESSION   DAOMUF010000089 DAOMUF010000000
VERSION     DAOMUF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2249_length_8218_cov_4.360529"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(472..1917)
                     /locus_tag="PLN77_06370"
     CDS             complement(472..1917)
                     /locus_tag="PLN77_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRM43937.1"
                     /translation="MKKEKSSAMGTPYTFEGRIPLKQAIPLGLQHVMAMFVGNLTPLL
                     IIMGACGLTADAGYGALRTALLQNAMTVAGIVTLVQMFSIGPIGGKVPIVMGTSSGFL
                     GVFKSVTAVLGQGALTYGAILGATIVGGLFEGVLGVCLKPLRKFFPSVVTGCVVMAIG
                     LSLIPVGINYLCGGSGTNDYGSIQNLFLGMVVLIVTLALKHFTNPKGILSTASILIGI
                     LVGYAVAIIMTMVLPHTGTAVLEDGSTVSYTYSWVVNFQQVKDASWFALPGIAGFGKL
                     AEVKPVFRVEAILPVAIMFIVTTVETVGDICACVESGMDREATDSELSGGIICDGLGS
                     SFAAALGVLPNTSFAQNVGIISMTKIVNRMALSCGAIFLILCGLCPKIAAFVSIMPQS
                     VLGGAAVMMFASILVSGIQLITKEPITSREITIVSVALGLGYGLGSTAGATSELPYYI
                     QLIFGGSGIVPAAVAAIVLNIIIPKEKKKAE"
     gene            complement(2205..2558)
                     /locus_tag="PLN77_06375"
     CDS             complement(2205..2558)
                     /locus_tag="PLN77_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRM43938.1"
                     /translation="MTLKDCYMQMGADYEETVQRLRSESLIQRIILKFPQDPSYQELC
                     QALVDGNIEVAFRAAHTLKGVCQNLGFKNLYEPSAELTERLRPLQETETDDLMQKVTE
                     EYNRTVKAIEDFQNA"
     gene            complement(2978..4312)
                     /gene="gdhA"
                     /locus_tag="PLN77_06380"
     CDS             complement(2978..4312)
                     /gene="gdhA"
                     /locus_tag="PLN77_06380"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRM43939.1"
                     /translation="MSYVDEVIESVVAKNPAEPEFHQAVKEVLESLRPVIEANEEKYR
                     KVALLERMVEPERQIKFRVPWVDDKGQAHVNTGYRVQFNSAIGPYKGGIRLHPSVNIG
                     IIKFLGFEQVFKNSLTSLPIGGGKGGSDFDPKGKSDREIMAFCQSFMTELCKYIGADT
                     DVPAGDIGTGAREIGFMFGQYKRIRGVYEGVLTGKGLSYGGSLARTEATGYGLLYLTQ
                     ELLKLNGIDIAGKTACVSGSGNVAIYAIEKATQLGVKVLTCSDSNGWVYDPDGIDVAA
                     LKEIKEVNRARLTEYKKYRPNSEYHEGRGVWVVKADLALPCATQNELLLEDAKALVEN
                     GCTAVCEGANMPTTLEATKYLQEHGVIFAPGKAANAGGVATSALEMSQNSERLSWTFE
                     EVDAKLKSIMINITHNMVDAAKRYGHEGDYVMGANIAGFEKVADAMMAQGIC"
     gene            complement(4662..5261)
                     /locus_tag="PLN77_06385"
     CDS             complement(4662..5261)
                     /locus_tag="PLN77_06385"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRM43940.1"
                     /translation="MGKQTWKAGNMLYPLPAVMVSVRGKNGEDNIITVAWAGTVCTNP
                     PMVSISVRPERYSYQMIKESGAFVINLTTEDLCYATDYCGVRSGRDVDKFKEMKLTKV
                     EAEEISAPMIGESPVNIECKVEQVLELGSHHMFLAKVVAVHADEAYMDERGRFDLNRA
                     KPIVYSHGEYFGIGKKQGTFGWSVKKKSKTPNIKTKNRR"
     gene            complement(5366..7021)
                     /locus_tag="PLN77_06390"
     CDS             complement(5366..7021)
                     /locus_tag="PLN77_06390"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HRM43941.1"
                     /translation="MKSNSRVYVIGHKNPDTDSICSAIAYADIKNRTDKTKTYMARRA
                     GQINEETEYVLKRFGVRAPGYLPNAGTQVKEIEIHEVPSVPGTISVKKAYSMMKNNNV
                     VTLPITSPDNDLQGVITVSDIAESYMDSYDSHVMSLARTQYRSIADTLDGSVIVGNEH
                     GYFIRGKVVVGAFHPDTMENYIEKDDLVILGNRAEDQLCAIEMDASCIIVGLGAKVTK
                     TIQKFAEEKCCVIISSPHDTYTIARLINQSIPVKYLMRRSNLITFNTEDFLDDIKEVM
                     KNQRHRDFPILNKKGKYVGTISRRNLIGNAGKKLILVDHNEESQAVDNVKEAEILEII
                     DHHRLGSLETMAPVMFRNEPVGCTGTIMYEIYKEKELEIAPNIAGLLCAAIISDTLMF
                     RSPTCTFLDKAAAEALADIAGISIPEFASEMFRAGSNLKDKSPEEIFYQDFKKFIMGD
                     VTFGVGQITSLDAGELESIKEQLLPQMESECGKHGIEMVFFMLTNIIEESTELLYYGS
                     GAKEVIEKAFEDLPVNEVSCELKGVVSRKKQLIPAFMMALQNQ"
     gene            7402..>8218
                     /locus_tag="PLN77_06395"
     CDS             7402..>8218
                     /locus_tag="PLN77_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRM43942.1"
                     /translation="MSEEKLTMDDFSAELEASFKKIEEGDMLTGTVISVDEKEVILDL
                     KYYAEGVIRAEDFSNEPGFSLKDAVQVGDEITATVIRKDDGHGNILLSRAEANDILAW
                     EKLAQYKEEKTVLDVKVKGIVNAGVIAYVEGIRGFIPASKLSLNYVEDLNEYLNKDIQ
                     VQVFDLDKEKDRLILSAREILREKADEERKEKISNVEVGLVTEGTVESLQPYGAFISL
                     GNGLSGLVHISQICAKRIKHPSAVLAVGDKVKVKVIAIKDGKLSLSMKALDDVA"
BASE COUNT         1979 a         2136 c         1687 g         2416 t
ORIGIN      
        1 gtgcttgcca gtaatgcaga aattactttc tttttcattt ggtgtcctcc ttgtgtttta
       61 cgttcttttt ggtgtgttcc gttgttgttt ttgtccaggc tgacgaatca tctctctttt
      121 acaatcatac gttttgccta aaactttcac attttcgtca cactttttat gcaatgatta
      181 tacctctctt ccacacgttt tggcaagcac tttttatata tttttcacaa tttatttcat
      241 ttttttactt catattgtcg attttataca ctatttttaa atttatagtc aatcttgact
      301 tttttgatat cggtatttct tttaatctca actaattttt cattcgtttt tccgtcattt
      361 tgtatatttt acacttgttc gaacgttttg tacagaaaaa gaacctctta atttttctcc
      421 agaaggctct ttccaggaaa actaagaggt tcttttttaa ttatctcttc tttattctgc
      481 ttttttcttc tcttttggaa taataatatt cagcacaatc gctgcaaccg ctgccggaac
      541 gataccggaa ccgccgaaga tcagctggat ataataagga agctcggaag ttgctcctgc
      601 tgtggagcca aggccgtagc caaggcccag tgcaacagaa acgatcgtga tctcacggct
      661 cgtaatcggc tcttttgtga tgagctggat accggaaacc agaattgacg caaacatcat
      721 aacggctgca ccgccgagta cggactgcgg cataatggat acaaatgctg caatcttcgg
      781 gcagagtccg cagaggatca ggaaaatggc tccgcaggaa agtgccatac ggtttacgat
      841 ttttgtcatc gaaataatgc cgacgttctg tgcaaaagaa gtattcggga gaacgccgag
      901 tgctgctgca aagctggatc cgagaccgtc gcagatgata ccgccggaaa gctcggaatc
      961 ggttgcctcg cggtccatac cgctctctac gcaggcgcag atatcgccga ccgtctcaac
     1021 ggttgttacg atgaacatga tcgctaccgg gagaattgcc tccacacgga agactggttt
     1081 tacctcagcc agttttccaa agcctgcaat accagggagt gcgaaccagg acgcatcttt
     1141 tacctgctgg aaattgacaa cccaggaata tgtataggaa acagtgcttc catcctcaag
     1201 aaccgccgtt ccggtatgcg gcagaaccat ggtcataata atagctaccg cgtagccgac
     1261 taaaataccg atcagaatgg aagccgtgct taagattccc ttcggatttg taaagtgctt
     1321 cagtgccagc gttacaatca gaacgaccat gccaaggaac aggttctgta tggaaccgta
     1381 atcgttcgtt ccggaaccgc cgcagagata attgattcca accggaatca gcgaaagacc
     1441 gatcgccata acgacgcagc cggtaacaac cgacgggaaa aatttacgaa gcggctttaa
     1501 acagacgcca agcacgccct caaacagacc gccgacaatt gtcgcaccaa ggatagcgcc
     1561 gtacgtcagc gcgccctgtc ccagcacggc tgtcacgctc ttgaatacgc cgaggaatcc
     1621 agaagatgta cccataacaa tcggcacttt accgccgatc ggtccaatcg aaaacatctg
     1681 taccagtgtt acaataccgg ctactgtcat tgcattctgc aaaagagccg tacgaagtgc
     1741 accgtagccc gcatccgccg tcagaccgca cgctcccatg atgatcagaa gcggggtaag
     1801 gtttcctacg aacatcgcca ttacgtgctg caatccaagc ggaatcgcct gcttcagcgg
     1861 aatccgtcct tcaaaggtgt acggggttcc catagctgaa cttttttctt ttttcattgc
     1921 ttttctcctg cttttgtaga tttgggctgc cttgcctttc ggtatggaat atgccctgca
     1981 cagccggaat ttttccggca aagcgtcctt gttttttctt atagatactc ataagttttt
     2041 ctttttaaac aagccttctt cataagctgt gcttttatgt aaaaagatta tcatgtttct
     2101 tttttcccgt caacccgcat cttgtacaga attccataaa aaggagacac cggtctgtgt
     2161 tcctcatctc acagatcaat gcctccttgt tttcttcctt caatttaagc gttctggaaa
     2221 tcctcaatcg cttttacggt gcggttatat tcttccgtca ccttctgcat cagatcatcc
     2281 gtctcggtct cctgcagcgg tcttaaccgc tcggtcagct cggcgcttgg ctcgtacaga
     2341 tttttaaagc caagattctg acacacgcct tttaatgtat gagctgcacg gaatgcaacc
     2401 tcaatatttc cgtccaccag tgcctggcaa agctcctgat agctcggatc ctgcggaaat
     2461 tttaaaatga tccgctgaat cagagattca ctgcgaagcc gctgaacggt ttcctcgtaa
     2521 tctgctccca tctgcatgta gcaatctttt aatgtcatct tctcttcctc ttccctgtat
     2581 gcaaattcat tacatattaa tgcatacaag ttctttatca atacataaat aattatattt
     2641 ctcatgcaag ctcttgtcaa cttctgcttt tcattatttt tgcattattt ttgtttacta
     2701 tgcgctctat ttctgaacat aaaatatctt ttttcttacg tttttatctt acctgtatat
     2761 tgcatttatg cataataata caacttactc gcatattctg tcgttttatt cacacggtac
     2821 cgcgtgaaag tctatttctt ctgtctagtc tcgttgcaaa atgtaatgtt ttttgtaccc
     2881 tgtaattttc gttatttttc tgtgtttctt ctatctttta tgcaaaaagg gacccccggt
     2941 cttaaccgga gatccctttg gatttctcaa aatcagatta gcagattccc tgagccatca
     3001 tagcgtcagc tactttctca aagcctgcga tgttagcgcc cataacatag tcgccctcat
     3061 ggccgtaacg ttttgcagca tcaaccatgt tgtgtgtgat gttgatcatg atgctcttca
     3121 gttttgcatc aacttcttcg aaggtccagg acagacgctc ggagttctgg gacatctcga
     3181 gtgcggatgt tgcaacacca cctgcgtttg cagcttttcc aggtgcgaag attacgccgt
     3241 gctcctgcag atatttggtt gcttccagag tagtaggcat gttagcgccc tcgcatactg
     3301 cagtgcatcc gttctcaaca agtgcttttg catcctcaag cagcagctcg ttctgagttg
     3361 cgcaaggaag agcaaggtct gcttttacaa cccatacgcc gcgtccttcg tggtactcgg
     3421 agtttggacg atatttcttg tactcggtca gacgggcacg atttacttct ttgatctctt
     3481 ttaatgcagc tacatcgatt ccgtctggat cgtaaaccca tccgttggag tcagagcagg
     3541 tgagaacttt cacaccaagc tgagtagctt tctcgattgc atagattgca acgtttccgg
     3601 aaccggatac acaagcggtc ttgcctgcga tgtcgatgcc gttcagtttc agcagctcct
     3661 gggtcaggta cagcagtccg tatccggtag cctctgttct tgctaaagat ccgccgtagc
     3721 ttaaaccttt accagtcaga acgccttcgt atacgccgcg gattctcttg tactgtccga
     3781 acatgaagcc gatctctctt gcgcctgttc cgatgtcacc agccggtacg tcggtgtctg
     3841 cgccgatata tttgcaaagc tctgtcataa agctctggca gaatgccatg atctctctgt
     3901 cagattttcc tttcggatcg aagtcagaac cacctttacc tccgccgatc ggaaggctgg
     3961 tcagtgagtt tttgaatacc tgctcgaatc ccaggaattt gataatacca atatttacag
     4021 aaggatgcag acggattcct cctttgtaag gtccgattgc actgttaaac tgtacacgat
     4081 atccggtgtt tacatgagcc tgtcctttat cgtctaccca cggcacacgg aatttgatct
     4141 gacgctctgg ctctaccatt ctctccagaa gagcgacttt tctgtatttt tcctcgtttg
     4201 cctcgataac cggacgaaga gattccagaa cctctttgac tgcctgatgg aattccggct
     4261 cagctgggtt ttttgcaact acggactcaa taacctcatc tacgtatgac atgttagttt
     4321 cctccttgaa atttctccct taatgtagtg cagaaagatt tctttaaaag atccatctgc
     4381 ggttctcatc agccttaaag cgtggagaaa atcatttccg cactctctcg cgtctccggt
     4441 ggattctccc gtcctccgga tcacttaatt ttataaggtc aacgattgtt tgatattata
     4501 gcataaaata tccggttttt gcaatatttc acgcaaaaaa ttcctaataa atcatgattt
     4561 tgtagcgttt tctaatattt acccagtatt tttgtcgatt ttgtatattt ttaccgaaag
     4621 tactataaaa tttcgcgtgc tgattcttta gaaaataaag attagcggcg gttttttgtt
     4681 tttatattag gtgttttcga ttttttcttc acgctccagc caaaggttcc ctgctttttc
     4741 ccgattccaa agtactctcc gtgggaatac acaatcggtt tcgcgcggtt cagatcgaag
     4801 cggccgcgct catccatgta tgcctcatcc gcatggacag ccacaacttt tgcaagaaac
     4861 atgtgatgcg agcccagttc gagcacctgc tccactttac actcgatatt aaccggcgac
     4921 tccccgatca tcggtgcgga aatctcctcc gcctcgactt tcgtcagttt catctctttg
     4981 aacttatcca catctcttcc cgagcgcacg ccgcagtagt cggtcgcata gcagaggtcc
     5041 tccgtcgtca ggttgatgac gaacgcaccg ctctctttga tcatctgata gctgtagcgt
     5101 tccggccgca ccgagatcga taccatcgga ggattcgtac acaccgtgcc ggcccaggca
     5161 acggtgatga tgttgtcctc cccgttcttt ccgcgcaccg acaccatgac cgccggcagc
     5221 ggatacagca tatttcccgc tttccatgtc tgttttccca ttcttatttc tttcctttcc
     5281 tctttttcac ccttttatga ccgaaaaaag cgtgccccgg atctgttttc aggcccggaa
     5341 ccacgctttt ttattttttc tgtttttact gattctgcag cgccatcata aatgccggaa
     5401 tcagctgttt ctttctcgac acaacaccct tcagctcgca gctgacctcg ttgaccggaa
     5461 gatcttcaaa agctttttcg atgacttcct ttgccccgct tccataatac agaagctctg
     5521 tagattcttc tataatattg gtcagcataa agaataccat ctcgatgccg tgttttccgc
     5581 actcgctttc catctgcggc agaagctgtt ccttgatgga ttccagctcg cccgcatcga
     5641 gggatgtaat ctggccgaca ccaaacgtaa catcgcccat gatgaatttt ttgaaatcct
     5701 ggtagaaaat ttcttccggt gatttatctt tcaggttgct tcctgcacgg aacatttcgg
     5761 atgcaaactc cggaatgctg atgcctgcaa tgtccgccag tgcctctgct gccgctttgt
     5821 caagaaacgt acacgtcggc gaccggaaca tcagcgtatc ggaaatgatg gctgcgcaga
     5881 gcagtcctgc gatgttcggc gcgatctcca gctctttttc cttataaatt tcatacataa
     5941 tcgttcccgt gcagcccacc ggctcattgc ggaacataac cggcgccatc gtctcaagtg
     6001 atcccagacg atgatggtcg atgatctcga gaatctccgc ctctttgacg ttatcgaccg
     6061 cctgtgattc ctcattgtga tcgacgagaa tcagtttctt tcctgcattt ccgatcaggt
     6121 tccgacggga aatcgttccc acatattttc ctttcttgtt cagaatcggg aaatcgcggt
     6181 gtctctgatt tttcataact tctttgatat catcgaggaa atcttcggtg ttgaacgtga
     6241 tcagattgct tctgcgcatc agatatttga ccggaatact ctggttaatc agacgcgcaa
     6301 tcgtatacgt gtcatgcgga ctgctgatga tcacacagca tttttcctct gcaaatttct
     6361 gaatcgtttt cgtcaccttt gcgccaagtc cgacgataat gcagcttgca tccatctcga
     6421 ttgcacagag ctggtcctcc gcacggtttc cgagaatgac aaggtcatct ttttcgatgt
     6481 agttctccat cgtatccgga tggaatgcgc cgacaacgac ttttccgcgg ataaaataac
     6541 catgttcatt tccgacaata accgaaccat ccaacgtatc cgcgatactg cggtattgcg
     6601 tccttgccag tgacatgaca tggctgtcat aagaatccat ataggattct gcgatatcac
     6661 tgaccgtaat cacgccctgc agatcattgt ccggcgaagt aatcggcagt gtgaccacgt
     6721 tgttgttttt catcatggaa tacgctttct tcacggaaat cgtgcccggt acgctcggca
     6781 cctcatggat ttcaatttcc tttacctgcg ttcccgcatt cggcagatac cccggtgcgc
     6841 gcacgccaaa gcgcttcagt acatactcgg tctcctcatt gatctgaccg gcacgccgcg
     6901 ccatgtaggt tttggtttta tctgtcctgt tcttgatatc cgcataggca atcgccgaac
     6961 aaatggaatc cgtatccgga tttttatgtc caattacata aactcttgaa tttgatttca
     7021 taatagtact cctcccttct gtatgtttgt ggtatacagt aagttcgtcc cggtagtttt
     7081 acttcttttt ttgtaacgac tacagttact gctcacttcg tgaccagtaa ccgccgccct
     7141 gcacatgcaa attcgctccg cgaagttccg ggcggccacc tgaatcactt tctcctgcag
     7201 ccgcgcagca agtgcgcgtc tcagtaattt tggtaacgac tacacacata ttaccatcct
     7261 tttttcatct ggtcaaagaa aatcttttat gttaagataa acgcagatag aaaaacgtgc
     7321 aaaattgaac ccgatttttt cataaatcca caattttcct ctcgttttcc tgttattctt
     7381 acgaaaaagg ggtgtttcat tatgtcagaa gaaaaattaa ccatggatga cttctccgca
     7441 gagctggaag cttccttcaa aaaaattgag gaaggcgaca tgctcaccgg aaccgtcatc
     7501 tccgtagatg aaaaagaggt gattctcgat ctgaaatact acgcagaggg cgtcatccgc
     7561 gccgaggact tcagcaacga gcctggcttc tccttaaaag acgctgttca ggtcggtgac
     7621 gaaatcaccg caaccgtgat ccgcaaagac gacggccacg gcaacattct tctctcccgc
     7681 gcagaggcaa acgatatcct cgcatgggaa aaactcgcac agtacaaaga agagaaaacc
     7741 gtactggatg taaaagtcaa aggaatcgta aacgccggtg ttatcgctta cgtcgaagga
     7801 atccgcggct tcatcccggc ttccaaactc tccctgaact acgtggaaga tctgaatgaa
     7861 tacttaaaca aagacattca ggtacaggtt tttgacctcg acaaagaaaa agaccgcctg
     7921 atcctctccg cccgcgaaat tctccgcgag aaagcagacg aagagcgcaa agagaaaatc
     7981 tccaacgtag aagtcggcct cgtcaccgaa ggaaccgttg agagcctgca gccatacggc
     8041 gccttcatca gcctcggaaa cggcctctcc ggcctcgtcc acatctccca gatctgcgcc
     8101 aaacgaatca aacatccatc cgcagtcctg gcagtcggcg ataaagtcaa agtaaaagtc
     8161 atcgccatca aagacggcaa actcagcctc tccatgaagg ccctcgacga cgtcgccg
//
LOCUS       DAOMUF010000090         8211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2253_length_8211_cov_4.267656, whole genome shotgun sequence.
ACCESSION   DAOMUF010000090 DAOMUF010000000
VERSION     DAOMUF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2253_length_8211_cov_4.267656"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..958
                     /locus_tag="PLN77_06400"
     CDS             23..958
                     /locus_tag="PLN77_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM43943.1"
                     /translation="MAKEKMKKKKGQKIAPAILWKQRWLLVMSVPFVIWLIIFKYIPL
                     WGWTMAFQEVKPATFALPIWERTWVGFKNFTDAFKDRLFTQTLINTLGVSFLDIAVGT
                     IAAIAFAVLLNEMFFMKFKKVTQTISYLPHFVSWVIIASIAKNIFNDGGVVDSIFGKN
                     LHLMSSNSPLIWVTIILINCWKEVGWNAIIYLSAMAGIDPGLYEAADMDGASRLQKIW
                     HITLPGIRPTIIVLLIMSIGGALNVGMERQMLLSNPLVEDHTMVLSWFSYKRGIGNSN
                     YGLGTAIGVFQSAVSVTLLLIANKIAGLVGEDKLM"
     gene            971..1834
                     /locus_tag="PLN77_06405"
     CDS             971..1834
                     /locus_tag="PLN77_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM43944.1"
                     /translation="MNKKAKKFDYVLAVIFVFLVIITIYPFLNVLAISFNDSTDTIKN
                     VNFIIPRKFTLSNYKYVFKDGGIAMPAVISVTRTIIGTIVGIVCTSMVAYTLSRRDFV
                     LRKPFQLLFVVTMYVGGGMIPEYLLIIRTLKLGNNFMVYILPGLMWVYNMILVRSYID
                     GLPMALQEAAKLDGANDFQIWYKVVMPLCKPVIATVGLYYAVGQWNSFMDTYLYAKKL
                     PTLQYKLYEIMNQATMKMDVHAGTNVQTAVTPMSVRMAVTIVATVPIVIVYPFLQKYF
                     IGGMTLGSVKD"
     gene            1937..3655
                     /locus_tag="PLN77_06410"
     CDS             1937..3655
                     /locus_tag="PLN77_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM43945.1"
                     /translation="MKRKVVSLMLVSAMVAGMLAGCGSDSGSSKGGSSTETGSAAEAS
                     SSGETADDADDKSPITFEYFNADGKNGNWDNPVAKAITEATGVTLDVSYPVASQGDAK
                     EDVALMIANDEYPDMIYAKGSATDLYQAGALIDMTDLIEKYGPNIKKMYGAEMEKLKW
                     SQDDPGIYQLSYAGVNQKTLTTGGSCQIQWAALKENDYKYPKTLDEYEKMIKSYLAAH
                     PKTEDGLDMIGITMSASDWHWMITLGNPAGLIADASPDNGQWIIDDEYNVHYKHVTDE
                     EKEYFKWLCRMYNEGILDPNFATQTDDDYIAKVASGRVVAITDAEWHYSQCEATLVAD
                     GKVDQTYVGLPVTLREDQVEKALLYQGTTVGWGIGITKSCEDPVRAIKFLDYLCSDEG
                     QILYHWGIEGENYFLDDDGQPYRTDEEVAKAQSDPDYAKNTGIDNYTGFPIYGTGSYS
                     EDGFPYTPTTKESVIANYNTAEKEGCEAMGFEMLTDMFAQPEEFDLLPYSALWAYQQP
                     QELAEKQTILDEIAWPGLVKCVTGTEDEFDANWESMVQELTDNGLADAEEAMTEFLAT
                     KLVDVE"
     gene            3851..6814
                     /locus_tag="PLN77_06415"
     CDS             3851..6814
                     /locus_tag="PLN77_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRM43946.1"
                     /translation="MTEKKEFQVNKNTPFWDASLTDQERIDWLLKEMTVEEKLGYLAS
                     SSPDLPRLGIPGVSVGGEAAHGVEGRNDQNGLGKPDVTTSFPQPIGMSASWDPELIRQ
                     AGEVTGTEARVVWHRHEGHGLSRWAPTVDLERDPRWGRNEEGYGEDPVLTGKMAGAYI
                     RGMQGDDPKYLRCAATLKHFYGNNTEVGRGWKNSSIDPRNKYELYLEPFRRCIEDGGA
                     EGIMTAYNKINGTIGILNPEVTDILKKQYGLRHVVSDGGAMGLVVNLHHYFGQHAETI
                     ATALKAGVDAMSDDPRMVEQAAREAYELGILKEEDMDRSIRCMMETKLRLGVYDRENL
                     NPYDRVTEDDIDSPKAREICKELSRESIVLLKNENGALPLDKALKAEDIAIVGPLGDA
                     WYQDWYGGTAPYRTTFLQGMEVLKQENITFADGLDRVVFRCDGKGLAVAEDGTLQMAD
                     EPDVFIKEYWGEGSYTFKSVRTGKYLGARLSESQGEKPKMGQIAADREEAFDWFVMEI
                     FHVEPQEDGSVVLTNRFHYPVYKDAEGFFSFEQTEGIPITMEVVENGIEKAVAAVRGK
                     KQVLLALGCNSVINAKEEIDRNTLELPEEQEMLLDRIAEVNPNTVLVLFTNYPYTLQK
                     AMEKLPAIIMSATGSQDMGSAMAEAVLGIYAPAGRLNMTWYESIDQLPDIDDYDIIKG
                     KRTYRYFDGKVLYPFGYGLTYTTFAYENYKVSLKDDRLLQISLDVRNTGDTASDEVVQ
                     IYGSALESCVKKPICQLLDFVRVKNIAPGETRHIALEIPVEELRFYDVISRRLMVEEG
                     TYEIYAGASCKDKAVSAEIFIPGGKRGVRDLSAFTAADHYDDYENMYLTEGHFNFKAV
                     RVQDETKDGVLVYRDCDLSDAAVLALHVKSERGGSVEAFVDGVSMGSFTGDTRTCEFR
                     SAPKLDRYAEEEVKERNRYREPVYEDVEISLADRPQTDGASEIRLVLKGDMRICYLRV
                     LKNKSTGKIQMGVAN"
     regulatory      6893..7021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7234..7875
                     /locus_tag="PLN77_06420"
     CDS             7234..7875
                     /locus_tag="PLN77_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM43947.1"
                     /translation="MNAQTTAKQTSKMSQIGKTRYMVQVAMLGAAAVVLMFFDIPLPF
                     APSFYKIDLSEVPVLIGAFAMGPLAGAAIELIKILLNLVMKGSTTAGVGEVANFLIGC
                     AYVMPAAWIYKTQKTKKNAMIGMAVGTVFLAAAGGLLNAFVLLPAYAAAFGMPMDALV
                     GMGSAVNKAITSLPTFVLFAVVPFNIIKGVVVSLVTMLLYKHISRLLKGEAMN"
BASE COUNT         2350 a         1750 c         2182 g         1929 t
ORIGIN      
        1 aacaacggaa aggatgggga gtatggcgaa ggaaaaaatg aaaaagaaaa aagggcagaa
       61 aatcgccccc gcaattttat ggaaacagcg ctggctgctt gtgatgtcag taccatttgt
      121 aatctggctg attatcttca agtacattcc gctgtgggga tggaccatgg catttcagga
      181 ggtaaaaccg gcaacgttcg cacttccgat ctgggagaga acctgggttg gatttaagaa
      241 ctttacagat gcatttaaag accgtctgtt cacacagaca ttaatcaaca cattgggagt
      301 aagcttcctt gacatcgcgg ttggaacgat cgcagcaatc gcatttgcgg ttcttctgaa
      361 cgagatgttc tttatgaagt tcaaaaaagt aacgcagacc atttcttatc tgccgcactt
      421 tgtatcctgg gttatcattg caagtattgc aaagaatatt ttcaacgacg gcggtgttgt
      481 tgacagtatc ttcggcaaga acctgcacct gatgagttcc aactcaccgc ttatctgggt
      541 aaccattatc ctgattaact gctggaaaga ggttggctgg aacgcaatta tttatctgtc
      601 cgccatggca ggtattgatc ctggtcttta cgaggccgct gatatggacg gtgcaagccg
      661 tctgcagaag atctggcaca tcactctgcc gggtatccga ccgaccatca tcgtactgct
      721 gattatgagc atcggtggcg ccctgaacgt aggtatggag cgtcagatgc ttcttagtaa
      781 cccgctggtt gaggatcaca cgatggtact ttcctggttc tcttataaga gaggtatcgg
      841 aaacagcaac tacggtcttg gtaccgcaat cggtgtattc cagtctgcag tcagcgttac
      901 cctgctgctg atcgccaata agattgccgg tctggtaggc gaagataaac ttatgtagga
      961 ggacgcgaat atgaacaaga aagcaaaaaa atttgactac gtactggcgg taatcttcgt
     1021 attcctcgtc atcatcacca tttatccgtt cctgaacgta ctggcgattt cgttcaacga
     1081 ttctacggat accattaaga atgtaaactt catcattccg agaaagttta cattaagcaa
     1141 ctataaatat gtatttaaag acggcggcat tgcaatgccg gctgtgattt ctgtaacaag
     1201 aaccatcatc ggaacaatcg taggtattgt atgtacctcg atggttgctt acacattaag
     1261 ccgcagagac ttcgtactcc gcaaaccatt ccagcttctg ttcgttgtta ccatgtatgt
     1321 cggtggcgga atgattccgg aataccttct gattatccgt accttaaagc tgggcaataa
     1381 cttcatggta tacattctgc ctggtctgat gtgggtttat aatatgattc tggttcgctc
     1441 ttatatcgac ggactgccga tggctctgca ggaggcagca aagctcgacg gagcaaacga
     1501 ttttcagatc tggtataaag tcgttatgcc gctttgtaaa ccggttatcg caaccgtagg
     1561 tctgtactat gcagtaggac agtggaactc cttcatggat acttacctgt atgcgaaaaa
     1621 actcccgact ctgcagtaca aactgtatga gattatgaac caggcgacca tgaaaatgga
     1681 cgttcatgca ggaaccaatg tacagacggc cgtaacacca atgtccgtac gtatggccgt
     1741 aacgattgta gcaactgtgc cgatcgttat tgtatatccg ttccttcaga aatactttat
     1801 cggcggtatg acacttggtt ctgttaaaga ctgataaaaa acagtattaa aaaattttta
     1861 gaaaaagatg atgtttattc ctgccaggcg aaagaaagag tgcaggatga cataaaccat
     1921 tttaaggagg gcttttatga aaagaaaagt agtatcactc atgttagtat ctgctatggt
     1981 ggccggaatg ctcgcaggat gcggttccga cagtggcagc agcaagggag ggtccagcac
     2041 agagactgga tcagcggcag aggcaagttc ttcaggcgag acagcagatg atgcagatga
     2101 caaatctccg attacctttg agtattttaa cgctgatggt aaaaacggca actgggataa
     2161 cccggtagca aaagcaatca cagaggcaac aggcgttaca cttgatgttt cctatccggt
     2221 agcatcccag ggtgatgcaa aagaagatgt tgcgctgatg atcgcaaatg atgagtatcc
     2281 ggacatgatt tatgcaaaag gctcagcaac ggatttgtat caggcaggcg cgctgattga
     2341 tatgacagac ctgattgaaa aatatggtcc aaacatcaaa aaaatgtacg gagcagagat
     2401 ggaaaaactg aaatggagcc aggatgatcc tggaatctat cagctttcct acgcaggtgt
     2461 aaaccagaaa accctgacaa caggcggaag ctgccagatt cagtgggctg cattaaaaga
     2521 gaacgattat aaatatccga agacactgga tgagtatgaa aaaatgatca agagctatct
     2581 tgcagcacat ccaaaaacag aagatggtct ggatatgatc ggtatcacca tgagtgcttc
     2641 tgactggcac tggatgatta cccttggaaa cccggcagga ctgatcgctg atgcatcccc
     2701 ggataatgga cagtggatca ttgacgacga gtacaacgta cattacaaac atgtaacaga
     2761 tgaagagaaa gaatacttca aatggctgtg ccgtatgtac aacgaaggta tcctggatcc
     2821 gaactttgca acgcagacag atgatgacta tattgcaaaa gttgccagcg gccgtgttgt
     2881 tgccatcacc gatgctgagt ggcattacag ccagtgcgaa gctactctgg tagcagacgg
     2941 aaaagtagat cagacttatg ttggacttcc tgtaacctta agagaagatc aggtagaaaa
     3001 agcccttctg tatcagggaa ctaccgttgg ctggggtatt ggaattacca agagctgtga
     3061 agatccggtt cgtgccatca aattccttga ctatctgtgc tccgatgaag gacagatcct
     3121 gtatcactgg ggtatcgaag gcgagaacta cttcctcgat gatgacggac agccatatcg
     3181 taccgatgaa gaagttgcaa aagctcagtc tgatccggat tatgcaaaga ataccggtat
     3241 tgacaactac actggattcc cgatctacgg aacaggttcc tactctgaag acggattccc
     3301 atacactcca actaccaaag agtctgttat tgcaaactac aatacagcag aaaaagaagg
     3361 atgcgaagcg atgggatttg agatgctgac cgacatgttt gcacagccgg aagagttcga
     3421 tcttcttcca tactctgcac tgtgggcata tcagcagcca caggaacttg ctgagaaaca
     3481 gactattctg gatgaaattg catggccagg actggttaag tgcgtaactg gtaccgaaga
     3541 tgagtttgat gcaaactggg agagcatggt acaggagctg acagacaatg gtctggcaga
     3601 tgcagaagaa gctatgacag agttccttgc taccaaactt gttgatgtag agtaagtaaa
     3661 gcaaaaacat aaggttttgc cgttgaacgg aaggcggcac aaaaacatgg tctgatatta
     3721 ttaaatgtca ggccatgttt taaaatacag cgacagtaat gggaaatgtg aaacattgta
     3781 aaaagataac agtatatgga aaatctgcaa acaatttcat gagacaaaat catagggagg
     3841 tttttgaaaa atgacagaaa agaaggagtt tcaggtaaac aaaaacacac cgttctggga
     3901 tgcatcactg acggatcagg aaagaatcga ctggctgtta aaagaaatga cggtcgaaga
     3961 aaaactggga tacctcgcca gctcttcccc ggatctgccg cgccttggca tcccgggagt
     4021 ctctgtgggc ggtgaggcgg cacacggcgt tgagggcaga aacgatcaga acggtcttgg
     4081 aaaaccggat gtgacaacat ccttcccgca gccgatcggt atgagtgcat cctgggaccc
     4141 ggagctgatc aggcaggccg gtgaagtgac gggaacggag gcgcgtgtcg tatggcaccg
     4201 ccatgagggc catggactca gcagatgggc tccgaccgtt gatcttgagc gcgatccgag
     4261 atggggaaga aacgaagaag gttacggaga ggatcctgtg ctgaccggaa aaatggccgg
     4321 tgcttatatc cggggaatgc agggcgatga tccgaagtat ctgcgctgtg cagcgacctt
     4381 aaagcatttc tatggaaaca acacggaggt cggccgcgga tggaaaaatt cttccattga
     4441 cccgcggaac aaatatgagc tgtatctgga gccattccgc agatgtattg aggatggcgg
     4501 cgcagagggc attatgacgg catataacaa gatcaacgga acgatcggta tcctgaatcc
     4561 ggaagtgacc gatattctga aaaaacagta cggtctccgt cacgttgtca gcgacggcgg
     4621 tgcgatggga cttgtcgtga atctgcatca ctatttcgga cagcatgcgg agacgatcgc
     4681 aacggcgctg aaagccggtg tggatgccat gtctgacgat ccgcgtatgg tagagcaggc
     4741 ggcacgcgag gcatatgagc ttggcattct gaaagaagag gatatggacc gctctattcg
     4801 ctgtatgatg gagacgaagc ttcgtctcgg cgtttatgat cgggaaaatc tgaatccgta
     4861 tgaccgtgtg accgaggacg atatcgattc cccgaaggca cgtgaaatct gtaaagagct
     4921 gagccgcgaa tcgatcgtac ttctgaaaaa tgagaacgga gcgctgccac ttgacaaagc
     4981 cctgaaagca gaagatattg ccattgtcgg accgcttggt gatgcatggt atcaggactg
     5041 gtacggcgga acggcaccat accgcacaac cttcctgcag gggatggaag tactgaagca
     5101 ggagaacatt acattcgcgg atggtctgga tcgcgttgtt ttccgctgtg atggaaaagg
     5161 tcttgccgta gcagaagacg gaacacttca gatggcagat gagccagatg tgtttatcaa
     5221 ggaatactgg ggtgagggaa gctatacgtt caagagtgtc cgtaccggaa aatacctcgg
     5281 cgcaagactc tcagagtcgc agggagaaaa gccgaaaatg ggacagattg cggcagaccg
     5341 ggaggaagcc ttcgactggt tcgtgatgga gattttccac gtggagccgc aggaggatgg
     5401 cagcgttgtg ctgaccaacc gtttccatta cccggtttat aaggatgcag agggattttt
     5461 ctccttcgag cagacggagg gaattccgat tacgatggag gttgtcgaaa acggaattga
     5521 aaaagcagtt gctgcagtcc ggggcaaaaa gcaggtttta cttgcgctcg gctgtaactc
     5581 cgtgatcaat gcgaaagagg agatcgaccg caacacactg gagctgccag aggagcagga
     5641 gatgctgctt gaccggatcg cagaggtcaa tccgaatacc gttctggtgc tgtttacgaa
     5701 ttatccgtac acacttcaga aagcgatgga aaaactgccg gcgatcatca tgtctgcaac
     5761 gggaagccag gatatgggaa gcgcaatggc agaggctgtg cttggaatct atgcaccggc
     5821 cggccgtctg aatatgacct ggtatgagag tatcgatcag ctgccggaca ttgacgatta
     5881 cgacatcatc aaaggaaaaa gaacgtaccg ctatttcgac gggaaggtat tgtatccatt
     5941 cggctatggc ctgacgtata ccacatttgc gtatgagaac tataaggttt ccttaaaaga
     6001 cgaccgtctg ctgcagatct ctctcgatgt gagaaatacg ggcgataccg caagcgacga
     6061 ggtcgtccag atctacggtt ccgctctgga atcctgcgtg aagaaaccaa tctgccagct
     6121 gcttgatttt gtccgtgtga aaaacattgc cccgggcgag acgagacaca tcgcactgga
     6181 aatcccggtg gaggagcttc gcttctacga tgtcatcagc cgcagactga tggtagaaga
     6241 aggaacgtat gaaatctatg cgggcgcatc ctgcaaggat aaagccgttt ccgcagaaat
     6301 ctttattccg ggcggaaaaa ggggagtacg cgatctgagt gcatttaccg cggcagacca
     6361 ctacgatgat tacgagaaca tgtatctgac ggaaggccac ttcaatttca aagcagtccg
     6421 cgtgcaggat gagacgaaag acggcgttct cgtttaccgt gactgtgatc tttccgatgc
     6481 tgctgttctg gcacttcatg tgaagagtga acgcggcggc agtgtggaag cctttgtgga
     6541 cggtgtttcg atgggaagct ttaccggtga cacaagaacc tgtgagttcc gcagcgcccc
     6601 gaaactggac cgctatgcgg aggaagaagt aaaagagcgc aaccggtacc gcgagccggt
     6661 ttatgaggat gttgaaattt ccctcgcaga cagaccgcag acggacggcg catctgagat
     6721 ccgtctcgtc ctgaagggag atatgcggat ctgctatctg cgtgtgctga aaaataagag
     6781 cacaggaaaa attcagatgg gcgtagcaaa ctaaccagat aatgtattgt ctgaaaaaag
     6841 tattgacaaa ttgtgtgaat atgaataaaa tattcctgta aaaacaaaat acggagatct
     6901 tcagggcagg gtgtgattcc ctactggcgg tatagtccgc gacccgctgc gatatcaaac
     6961 agcggctgac tcggtgcaac tccgggacca acagttaaag tctggatgaa agaagacgaa
     7021 ataaagttta tttgcatgga aatatttttg gtgccctgaa ttcaagaaaa ggaattcagg
     7081 gcactttttt tcgcgggcaa cgagccaaag tccgtgcttg cacgagacct tggcgactgc
     7141 cgcgaaaacg agttcaaatt cgtggagcgt atttggactc gttacttccg gcagaaccgg
     7201 tcaagatctc cagaaaaaag gagaaatgca gttatgaatg cacagacaac agcaaaacag
     7261 acatcaaaga tgtcgcaaat cggaaaaacc cgttacatgg tacaggtggc aatgctcggc
     7321 gccgccgcag tcgtattgat gttttttgac atcccgcttc cgtttgcccc gagtttctac
     7381 aagattgatt taagcgaagt tccggtgctg atcggagcct ttgcgatggg acctcttgcc
     7441 ggagctgcga ttgagctgat taagattctg ctgaaccttg tcatgaaagg aagtaccacc
     7501 gcaggtgttg gcgaagtagc aaacttcctg atcggctgtg cgtatgttat gccggccgca
     7561 tggatctaca aaacacagaa aaccaagaaa aatgccatga tcggtatggc agtcggaacc
     7621 gtatttctgg cagcagccgg cggtcttctg aacgcttttg tactgcttcc ggcttatgca
     7681 gcagcctttg gaatgccgat ggatgccctt gtgggaatgg gatccgcagt caacaaagca
     7741 attacaagcc ttccgacctt tgttctgttt gccgttgtac cgtttaatat catcaaaggt
     7801 gttgtggtat cactggtaac catgcttctg tacaagcaca tcagcagact gctgaagggt
     7861 gaggcgatga attagttcat catttccgaa atcatttccg taaacggaga atccaataaa
     7921 tacgttgtag agagacggac aaaatgcaga gagaaatcct ctgttttttg tccgtctttt
     7981 aaaattttcg gggaatggcg gcaatggatt gcccacacag gtcttttagt agtacaatga
     8041 agcataatcg ataaaagaaa acgcgggcag aatatgaagg aattttataa aatgattgaa
     8101 aagaaaatcc gggaatccgg ctatccgcgt ccgatcgtgg gaagcgatgt ttacgatgac
     8161 atctgcggcc aatgtctgaa gatcactcat ggcgcgtgtc acggaaccgg c
//
LOCUS       DAOMUF010000091         8206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2254_length_8206_cov_3.456876, whole genome shotgun sequence.
ACCESSION   DAOMUF010000091 DAOMUF010000000
VERSION     DAOMUF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2254_length_8206_cov_3.456876"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /gene="aguA"
                     /locus_tag="PLN77_06425"
     CDS             <1..989
                     /gene="aguA"
                     /locus_tag="PLN77_06425"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HRM43948.1"
                     /translation="AAFAEIAEAIAAYEQVYLFVRPQDKASAQELLSSDRIHLIEAST
                     EDAWARDTGATFVVNDQGGRRGIQWEFNAWGGQYDGLYSHFEHDREVPERICNFLGDS
                     FYNARPFVLEGGSIHVDGEGTLLTTEECLLSPGRNPSLTKAEIEEKLRQYLSVEKIIW
                     LPFGIYNDETNGHIDNMCCFVKPGEVLLAWTDDENDPQYARSRAAFDLLSHTVDAKGR
                     SFVIHKLPIPKHPICITEEDLLGYDFEAGEDQREAGERLAASYINFYLANHCVLLPRF
                     GDENDTVAAEILGKCFPDRRILPVDARVILTGGGNIHCITQQIPAKKRGNRL"
     gene            986..1864
                     /gene="aguB"
                     /locus_tag="PLN77_06430"
     CDS             986..1864
                     /gene="aguB"
                     /locus_tag="PLN77_06430"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HRM43949.1"
                     /translation="MKNIKIAALQFSCCSQVQENIDKAETMVREAASNGANLILLPEL
                     FERPYFCQEKRYDYYDYALPLTENPAVRRFQAVAGELGVVIPVSFYERDVQRLFNSIA
                     MIDADGSILGVYRKTHIPDDHFYQEKFYFTPGDTGFKVFETRFGTVGVGICWDQWFPE
                     TARCMAVQGAEILLYPTAIGSEPILNVDSSGHWRRVMQGHAAANLIPVAAANRIGTET
                     VTPCEENGGQSSSLTFYGSSFIADATGELVASAGKDTEEILYASFDLDALREERFSWG
                     LFRDRRPETYRIMTKQ"
     gene            1943..3340
                     /locus_tag="PLN77_06435"
     CDS             1943..3340
                     /locus_tag="PLN77_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRM43950.1"
                     /translation="MQYGVLCLLPPAAMLIFALKTKKSFEALIFGTLVAYLIMYKAAF
                     IGPWCDLLLSEISNADNQYILLLCGLFGGFIFLLREAKGTLGFSNLLSRFCKNERITM
                     MMSVFLGIIIFVDDYLNIMTVGTCMKDVCDKRKVPRQALAYIIDSTGAPVCALIPFST
                     WVVFYSGVFIQEPGILNMGFSTGMSVYLKVLPYMFYPMTALLVVVLFACKLMPKLGKM
                     KDAYTDLKKKEQTPEHPKMHNAPSVDIAMAGMSVDYPPENTNAGNILDFLIPIGILIG
                     VTVATQDMMQAIILALASCMILYLPRKKMTFTRYVELFFAGFADILPVLAILLASFMI
                     KTACGEMGLSEYVINLCVPYMNAKTLAAIIFVVIAVLTFVTSSFWGIEAVAVSIVVPL
                     AIALDANVLLALGAVVSGGVFGSHACFYSDATVLSSATCEIDNMSHAVSQFPYVLISA
                     ALAVAGFLICGIFAL"
     gene            complement(3415..3816)
                     /gene="atpC"
                     /locus_tag="PLN77_06440"
     CDS             complement(3415..3816)
                     /gene="atpC"
                     /locus_tag="PLN77_06440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRM43951.1"
                     /translation="MSTFPLRVGTPDGLLYEGDVARVVCRTISGDLAILPGHCNYCTA
                     LGMGEAHIVLEDGTRRNAACIGGMLSMMNGVCRVLATTREWEEDIDAERAEAAKHRAE
                     QALANGGLTDKEYRIAEAKLQRALVRLSVKE"
     gene            complement(3831..5231)
                     /gene="atpD"
                     /locus_tag="PLN77_06445"
     CDS             complement(3831..5231)
                     /gene="atpD"
                     /locus_tag="PLN77_06445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRM43952.1"
                     /translation="MSEKHIGEVVQVTGPVLDIRFPEGELPALLNAIEIDNHGEKLVV
                     EVAQQTGDNMVRCIAMKSTDGLVRGTKAVDTGGPIAVPVGEECLGRVFNLLGETVDNK
                     PAPETAEKWPIHRDPPSYEEQVPATEILETGIKVVDLICPYAKGGKIGLFGGAGVGKT
                     VLIMELIHNVATAHGGLSVFTGVGERTREGNDLYNEMKESGVIDKTALVYGQMNEPPG
                     ARMRVGLSGLTMAEYFRDVKNQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTL
                     ATEMGALQERITSTRKGSITSVQAVYVPADDLTDPAPATTFTHLDATTVLSREISSQG
                     IYPAVDPLDSTSRILTPEIVGHEHYEVARAVQQVLQRYKELQDIIAIMGMDELSEEDK
                     LTVSRARKVQRFLSQSFSVAEQFTGMEGKYVPLKETLRGFRMILDGECDDLPESCFLF
                     AGTIDEVFEKAKALNQ"
     gene            complement(5267..6133)
                     /gene="atpG"
                     /locus_tag="PLN77_06450"
     CDS             complement(5267..6133)
                     /gene="atpG"
                     /locus_tag="PLN77_06450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRM43953.1"
                     /translation="MAANMKAVKLRIKSVESTMQITKAMELVASSKLRKAKERADTCR
                     PYFQELYRTLQDIAKGNTEFYSIYAKEAKNEKCCYVLVAGDRGLAGGYNANLFRCFEA
                     DAKGKEICVVPVGKKAVEYAKSRKLDILTDSFAEIANLSVADSFEIARALCREFRKGT
                     FGHVELCYTKFVSMLSQMPVSVPILPLIDIAKEDGEDPEAKKIRNLILYEPNGVEVFE
                     AIVPEYLGGLIYGGICESVASELAARRTAMEAATGNAEEMIEQLNLYYNRARQASITQ
                     EITEIVGGAEGV"
     gene            complement(6152..7681)
                     /gene="atpA"
                     /locus_tag="PLN77_06455"
     CDS             complement(6152..7681)
                     /gene="atpA"
                     /locus_tag="PLN77_06455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRM43954.1"
                     /translation="MQLKPEEISKVIRSQIKYYENAIRQDETGTVLMVGDGIARASGL
                     SNCMAGELLEFEDGSYGMAQNLEENSVSVVLFGSGENISEGQLVKRTGKVVSVPVGEG
                     MIGRVVNALGQPIDGAGPITAAEYRPIESPAPGICERKGVNQPLQTGIKAIDSMVPIG
                     RGQRELIIGDRQTGKTTIATDTILNQKGKDVICIYVAIGQKRSTVASLVENLEKNGAM
                     AYTIVVAATASEASPLQYIVPYSGCAMGEYFMYQGKDVLIIYDDLSKHAVAYRALSLL
                     IRRPPGREAYPGDVFYLHSRLLERAAKLDDEHGGGSMTALPIIETQAGDISAYIPTNV
                     ISITDGQIFLETELFHSGVMPAVNPGVSVSRVGGNAQIKAMKKVAGTLKLIYSQYREL
                     ASFAQFGSDLDADTKARLEQGVRIVEVLKQNQNAPVPVEKQVAILYAVTKGILSKVAA
                     EDVRAYEDGLYTWLDTDAEGAAVMQEISATGKLEADTEEKLKSTLESYTENFINTRPA
                     K"
     gene            complement(7691..>8206)
                     /gene="atpH"
                     /locus_tag="PLN77_06460"
     CDS             complement(7691..>8206)
                     /gene="atpH"
                     /locus_tag="PLN77_06460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRM43955.1"
                     /translation="GGSLYELAQEEHLEEEILSEMKEIRALFRENPEYPRLLSEPSIP
                     RKERIGLIDAAFGSQAEPYLVNFIKLLCEKNLLREFGGCQEAFQRRYFAAHNIAEAVV
                     TSAASLSKEQEELLKKKLETMSGKTVVMELKTDPSVLAGVRVELDGKLFDGTVKGRLS
                     ELSRRISDTVV"
BASE COUNT         1788 a         2278 c         1936 g         2204 t
ORIGIN      
        1 gagcggcatt tgcagaaatt gcagaagcaa ttgccgctta tgagcaggtt tatcttttcg
       61 tccggccaca ggataaagca tcggcacagg aactgctttc ctccgaccgg attcatctga
      121 tcgaagcctc gacagaggat gcctgggcaa gagataccgg cgctacgttt gtggtcaacg
      181 atcagggcgg gcgaagaggc attcaatggg aattcaacgc ctggggcggc cagtatgacg
      241 gtctgtactc ccactttgaa catgaccgtg aggttccgga acgaatctgc aattttctcg
      301 gagattcctt ctataatgcc cgcccctttg ttctggaagg cggctccatc catgttgacg
      361 gggaaggaac cctgcttacg accgaagaat gtctgctgag tcctgggcgc aatccttctc
      421 ttacaaaagc ggaaattgaa gaaaagctgc gtcagtatct ttcggtcgag aaaatcatct
      481 ggctgccttt tggtatttac aacgatgaaa ccaacggcca tatcgataat atgtgctgct
      541 ttgtaaagcc aggagaagta cttcttgcct ggacagatga cgaaaacgat cctcagtatg
      601 ccagaagcag ggccgctttt gatcttctct cccataccgt tgacgcgaag ggacgaagct
      661 ttgtaattca caagctgccc attccaaagc atcccatctg tattacagaa gaagatcttc
      721 tcggctatga ttttgaagct ggcgaagatc agcgggaagc cggggaacgc cttgccgcca
      781 gctatatcaa tttttatctt gccaatcact gtgtccttct tccccgtttc ggcgatgaaa
      841 acgataccgt ggctgcagaa attctcggaa aatgctttcc ggatcgacgg atcctccctg
      901 tcgatgcacg cgtcattctg accggcggag gcaacattca ctgtattacc cagcagattc
      961 cggcaaagaa aagaggaaac agattatgaa aaatataaaa attgccgcac tgcagttttc
     1021 atgctgcagc caggtacagg aaaatataga caaagcagag accatggtcc gtgaagccgc
     1081 ttccaacggc gccaatctca tccttcttcc tgaattattt gaaagaccgt atttttgtca
     1141 ggaaaagcga tacgattatt atgattatgc cctgcctttg acagaaaatc ccgctgtccg
     1201 gcgttttcag gccgttgccg gagaacttgg ggtcgtgatt ccggtcagct tttatgaaag
     1261 agatgttcag cgcctgttta attccattgc catgattgat gcagacggat ccattcttgg
     1321 cgtttaccgc aagacccata tcccggacga tcatttttat caggaaaaat tctatttcac
     1381 tccgggagat acgggattca aagtctttga aacacgtttt ggaaccgtcg gcgtcggcat
     1441 ctgctgggat cagtggtttc cggaaacagc acgatgtatg gctgttcagg gagctgaaat
     1501 cctgctctat cctaccgcga tcggaagtga gccgatcctg aatgtagaca gcagcgggca
     1561 ctggcgacgc gtcatgcagg gacatgctgc cgcaaatctt attccggttg ctgctgcaaa
     1621 ccggattggt accgaaaccg taacgccctg tgaagaaaat ggcggccagt cctcttctct
     1681 tactttctac ggctcttcct ttattgccga tgcaaccggt gaactcgtgg catcggcagg
     1741 aaaagatacc gaggaaatcc tgtacgcctc cttcgatctg gatgccctgc gcgaagaacg
     1801 tttttcctgg gggcttttcc gtgaccggcg gccggaaact tatcgtatca tgacgaaaca
     1861 gtaaagctct gtctcttcct gttgtaactc ttcctacagc ggtgcgctgc atcacatatt
     1921 ctaactacaa aggagaaccg ctatgcaata tggagtttta tgtctgctgc cgcctgcggc
     1981 gatgctgatt tttgcattaa aaacgaaaaa atctttcgaa gctctgattt tcggaaccct
     2041 ggttgcctat ctcatcatgt acaaagcagc ttttatcgga ccctggtgtg atctgctgct
     2101 ttcggagatc agcaacgcag ataaccagta tattcttctt ctctgcggac tgttcggagg
     2161 cttcatcttc ctgctccgcg aggcaaaagg aacacttggt ttttccaacc tgttaagccg
     2221 cttctgtaaa aatgaacgga tcaccatgat gatgtcggta tttctcggaa tcattatttt
     2281 tgtcgatgat tatctcaaca tcatgaccgt tggaacctgt atgaaggatg tctgtgataa
     2341 acgaaaagtc ccacgccagg cccttgccta tatcattgac tcaaccggtg cacctgtctg
     2401 tgcactgatt cccttttcca cctgggtagt tttttattcc ggtgttttta ttcaggaacc
     2461 gggcattctc aatatgggct tttccaccgg tatgagtgtt tacttaaaag tgcttcctta
     2521 tatgttttat ccgatgactg cacttctggt tgtggttctg tttgcctgca aactgatgcc
     2581 aaagctcgga aaaatgaaag atgcctatac cgatctgaaa aagaaagagc agacaccgga
     2641 gcatccaaaa atgcacaatg ccccttccgt tgacattgcc atggccggaa tgagcgttga
     2701 ttacccgccc gaaaatacca atgccggaaa tatactggat tttctgatcc ccatcggaat
     2761 tctgatcggt gtgacagtag ccacacagga tatgatgcag gcgattattc ttgctcttgc
     2821 ttcctgtatg attctttatc ttccgcggaa aaaaatgacc tttacgcggt acgtggaact
     2881 gttctttgcc ggctttgcag atattcttcc ggttctcgcc attcttcttg catcctttat
     2941 gatcaagact gcctgcggcg aaatgggact ctcggaatac gtcatcaacc tgtgtgttcc
     3001 gtatatgaat gccaaaacac tggcagctat tatttttgtt gtcattgcag ttctgacctt
     3061 tgtcaccagc tctttctggg gaattgaggc cgtagccgtt tccatcgttg ttccgcttgc
     3121 catcgccctt gatgctaatg tacttctggc gcttggcgct gtcgtatccg gcggtgtttt
     3181 cggaagccat gcatgcttct attccgacgc aacggttctt tcctccgcga cctgcgagat
     3241 tgacaatatg tcacatgccg tgtcgcagtt tccttacgtc ctgatcagtg ctgctctcgc
     3301 cgtagcggga tttttaatct gcggaatttt tgctctttaa aaagcaccct taaaataaaa
     3361 aaagtcttcg acgatcggat tttcccagat tcgtcgaagg ctttttttgt ttttttattc
     3421 ttttacactc aaccgcacca gcgcacgctg cagcttggct tcggcaatac ggtattcttt
     3481 gtctgtcaga ccgccgtttg ccagcgcctg ctcggccctg tgtttggcag cctcggcgcg
     3541 ctctgcatca atatcctctt cccactccct ggttgttgcc aggacacggc agaccccgtt
     3601 catcatggaa agcattccgc cgatgcaggc ggcatttctt ctcgttccgt cctccagcac
     3661 aatatgcgcc tcgcccatac caagtgcagt gcagtaattg cagtgcccag gcagaattgc
     3721 caggtcgccg ctgatggttc tgcagacaac gcgcgctacg tcaccctcat agagaagtcc
     3781 gtccggcgtg ccgacacgta aaggaaatgt actcatacgt cattcctcct ttactgattc
     3841 agtgcttttg ctttttcaaa tacctcgtcg atggtacctg caaacaggaa acagctctcc
     3901 ggaagatcgt cgcactcgcc gtcaaggatc atgcggaatc cgcgcagagt ttcttttaac
     3961 ggcacatatt ttccttccat accggtaaac tgctccgcta ccgagaagct ctgggacagg
     4021 aaacgctgta ccttacgggc acggctgacc gtcagcttat cctcctcgga cagctcatcc
     4081 atacccatga tggcgatgat atcctgcagc tctttgtatc tctgcagcac ctgctgtacg
     4141 gcacgtgcta cctcatagtg ctcatggcct acgatttccg gtgtcaggat acggctggta
     4201 gaatcgagcg gatcaacagc cgggtaaata ccctggctgg agatttcacg ggaaagtacc
     4261 gtcgtggcat ccagatgggt aaacgttgtt gccggagccg ggtcggtcag gtcatcggcc
     4321 ggcacgtaaa cagcctgtac cgatgtgatg gatccctttc ttgtggaagt aatacgctcc
     4381 tgcagtgcgc ccatctcggt tgccagcgtc ggctggtatc ctactgcgga cggcatacgg
     4441 ccaagcagag ccgaaacctc ggaacctgcc tgcgtaaatc ggaaaatatt atcaatgaac
     4501 agcagcacat cctgattttt cacgtcacgg aaatattctg ccatggtaag gccggaaagt
     4561 ccgacacgca tacgcgctcc cggcggctcg ttcatctgtc catagaccag tgctgttttg
     4621 tcgataacac cgctctcttt catctcgttg taaaggtcat ttccttcacg ggtacgctct
     4681 ccgacaccgg tgaaaacgga aagtccgccg tgtgccgttg ccacgttgtg gatcagctcc
     4741 atgataagga ctgtttttcc aacgccggca ccgccgaaca gaccgatttt tccgcccttg
     4801 gcatacgggc agatcaggtc aacgaccttg attccggttt cgaggatctc cgttgccgga
     4861 acctgttcct cataggacgg cggatcgcgg tggatcggcc atttttctgc tgtttccggt
     4921 gccggcttat tgtctacggt ctcaccgagc aggttgaaca cgcggcctaa gcactcctct
     4981 ccgaccggaa cggcgatcgg gccgcccgtg tcgactgctt tcgttccgcg gacaagtccg
     5041 tctgtggact tcatggcaat gcagcgaacc atgttgtcgc ccgtctgctg cgccacttcc
     5101 acaaccagct tctcgccgtg gttgtctatc tcgatggcat tcagaagtgc cggcagctct
     5161 ccttccggaa aacggatgtc cagaacagga cccgttacct gcacaacctc gccaatgtgt
     5221 ttttcactca tttaacgact tctcctcact tttcttgttt ccactcttac acgccttccg
     5281 caccgccgac gatctccgtg atctcctgcg taatgcttgc ctgacgggca cggttgtaat
     5341 acagattgag ctgttcaatc atctcctctg catttcccgt tgccgcctcc attgcggtac
     5401 ggcgtgccgc aagctcactt gccacactct cacagatacc gccgtagatc aggccgccta
     5461 agtattccgg cacaatcgct tcgaatacct cgacaccgtt tggctcatac aggatcagat
     5521 tccggatttt tttcgcttcc ggatcctctc cgtcctcttt tgcaatgtca atcagaggca
     5581 ggatcggcac ggaaaccggc atctgcgaca gcatcgacac gaattttgtg taacaaagct
     5641 ccacatgtcc gaaggtccct ttccggaact cacggcacag tgcgcgcgcg atctcaaagc
     5701 tgtctgccac ggaaagattt gcgatctcgg caaagctgtc cgtcagaatg tccagcttcc
     5761 gggattttgc atactccact gcctttttgc cgaccggaac cacacagatc tcttttcctt
     5821 tggcatctgc ctcaaagcat cggaacagat tcgcgttgta tccgcccgca aggccgcggt
     5881 ctccggcaac cagcacgtag cagcatttct cgttttttgc ctcttttgca taaatggaat
     5941 aaaattccgt gtttcctttt gcaatatcct gcaaagtgcg gtacagctcc tgaaaatacg
     6001 gacggcaggt atcagcccgc tccttcgctt ttcgcagctt ggaggacgct acaagttcca
     6061 tcgccttggt aatctgcatc gtgctttcga cacttttgat ccgcagcttt acggctttca
     6121 tgtttgcagc cataggctcc tccttctttc cttattttgc aggtcttgta ttgataaagt
     6181 tttcggtgta cgattcgagc gtacttttca gcttctcctc ggtatctgcc tcaagttttc
     6241 cggttgcgga aatctcctgc atcacggcag caccctctgc atcggtatca agccaggtat
     6301 acagtccgtc ctcataggcg cggacatcct cagctgcaac ctttgacaga attcctttcg
     6361 ttaccgcata aagaatggcc acctgttttt ctaccggaac cggtgcattc tggttctgtt
     6421 ttaatacttc cacgatacgc acaccctgct ccagacgcgc ctttgtatcg gcatccagat
     6481 cggatccgaa ctgcgcaaag cttgccagct cgcggtactg ggaatagatc agtttcagag
     6541 ttccggctac cttcttcatc gccttaatct gcgcatttcc accgacacgg gatacggata
     6601 cgccagggtt taccgccggc ataacgcccg agtggaacag ctctgtctca aggaaaatct
     6661 ggccgtctgt gatggagatg acgttcgtcg gaatataggc ggaaatatcg cctgcctggg
     6721 tctcaatgat cggcagggcc gtcatggatc cgccgccgtg ttcatcgtcc agctttgcgg
     6781 cacgctccag aagtctggaa tggagataga aaacatctcc cggataagcc tcacgtcccg
     6841 gcggacggcg gatcagcagg gagagtgcac ggtatgcaac cgcgtgctta cttaagtcat
     6901 cataaatgat cagcacatct tttccctgat acataaagta ctcgcccatg gcacagccgg
     6961 aatacggtac gatatactgc agcgggctcg cttcggatgc cgtcgctgcc acgacgatgg
     7021 tgtacgccat tgcgccgttc ttctcaagat tttctactaa ggacgctact gtcgaacgtt
     7081 tctgtccgat ggcaacgtag atacagatca catccttgcc tttctggttc agaatggtat
     7141 cggttgcgat cgttgtcttt ccggtctgac ggtctccgat gatcagctct cgctgacctc
     7201 ttccgatcgg aaccatggaa tcgatcgctt taataccggt ctgcagcggc tgattaacac
     7261 cttttctctc acagatgccc ggtgccgggc tttcaatcgg acggtactct gctgccgtaa
     7321 tcgggcctgc gccgtcgatt ggctggccga gcgcgttgac aacgcgtccg atcatgcctt
     7381 ctccgaccgg aaccgaaaca acctttcccg ttctttttac aagctgtccc tcgctgatgt
     7441 tctcgccgga accgaataaa acgacagata cgctgttttc ctcgaggttc tgtgccattc
     7501 catagcttcc atcctcaaat tccagcagct cgcctgccat gcagttcgaa agaccgctgg
     7561 ctcttgcgat tccgtctccg accatcagaa cggtgcctgt ctcatcctgc cggatcgcat
     7621 tttcataata tttgatctgg ctgcggatga ccttggatat ttcttctggt ttcagttgca
     7681 ttttttcctc ctacaccact gtatcgctga ttcttctgga aagctcggac aaacgtccct
     7741 ttacggttcc gtcaaacagc tttccgtcga gctccacgcg gacacccgcc agaacggacg
     7801 gatcggtttt cagttccatc acgaccgttt ttccgctcat cgtttccagt tttttcttca
     7861 gaagctcttc ctgttccttc gacagggatg ctgcgctcgt cacaactgcc tcggcaatgt
     7921 tgtgcgccgc aaagtatctg cgctgaaacg cttcctggca tccgccgaat tcccggagaa
     7981 gatttttttc acacagcagt ttgataaaat tgaccaaata cggttccgcc tggctgccaa
     8041 atgccgcgtc gatcagcccg atccgctctt ttcgcggaat cgaaggctcc gataaaaggc
     8101 gcggatactc cggattttcc cggaacagag ctcggatctc cttcatctcg gaaaggattt
     8161 cctcttcgag atgctcctcc tgggccagct catacaggct cccacc
//
LOCUS       DAOMUF010000092         8201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2260_length_8201_cov_3.687699, whole genome shotgun sequence.
ACCESSION   DAOMUF010000092 DAOMUF010000000
VERSION     DAOMUF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2260_length_8201_cov_3.687699"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..371)
                     /gene="trxA"
                     /locus_tag="PLN77_06465"
     CDS             complement(54..371)
                     /gene="trxA"
                     /locus_tag="PLN77_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRM43956.1"
                     /translation="MSLIHVTSENFEKALQAAPGVAVVDFFATWCGPCKMLAPVIEEA
                     AEDMPEIQFYKVDVDEAPELARSFKVMSVPTLAFFKNGELVKKNVGAVSYEELEELIN
                     SVK"
     gene            complement(499..1347)
                     /gene="nudC"
                     /locus_tag="PLN77_06470"
     CDS             complement(499..1347)
                     /gene="nudC"
                     /locus_tag="PLN77_06470"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRM43957.1"
                     /translation="MIQDIAPHKYYVDYQPSVPNDTDLVLIYGHHTILCNFEDPEICY
                     PSIAEIAAVFPEIKEKAKFLFRIDETDYYELRSPQLPEFGIWKYENTLILRSAVPGWL
                     GFAGITGYQIHTWYTDHTYCGRCGTKMHAPGNERAMQCPSCGALSYPVICPSVIVAIT
                     DGDRLLLTKYAPSHSSHRQYALVAGYTEVGETLEQTVHREVMEEVGLKVKNLRYYKSQ
                     PWSFSGSLLAGFYCDVDGDPSITLDREELSVAEWFDRDSLPETPNLISLTGEMIECFR
                     QGKEPK"
     gene            complement(1429..1500)
                     /locus_tag="PLN77_06475"
     tRNA            complement(1429..1500)
                     /locus_tag="PLN77_06475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1466..1468),aa:Gly,seq:gcc)
     gene            complement(1521..1591)
                     /locus_tag="PLN77_06480"
     tRNA            complement(1521..1591)
                     /locus_tag="PLN77_06480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1557..1559),aa:Gly,seq:tcc)
     gene            2104..2559
                     /locus_tag="PLN77_06485"
     CDS             2104..2559
                     /locus_tag="PLN77_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM43958.1"
                     /translation="MDAIDYKILKILQKNARETASNISKEIHLSVSAVIERIRKMEET
                     GVIKEYTIIVDEKKTGNEMTALMEVSLEHPKFFDSFADAIQKLEYIVSCYYQTGDFDL
                     IIRISCHSSDELEEIHRQVMSLPGVDATRTHVVLKNVKNIYSAIRRPDK"
     gene            2671..3795
                     /gene="alr"
                     /locus_tag="PLN77_06490"
     CDS             2671..3795
                     /gene="alr"
                     /locus_tag="PLN77_06490"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRM43959.1"
                     /translation="MNRYLRCYAEISLEAIGHNIREVKKRLPEGVKLLGVVKANAYGH
                     GAVPVASYLENQVDYFATATIEEAVELRENGISAPILILGYVSPSQYGDLVEYDITQT
                     IDSYAQALALEKEAARQNRKAKAHLAVDTGMTRIGFQVTEHDADEAAKIADLPHIELE
                     GMFTHFSCADQEDKTYCSMQMEKYDKMTALLAERGVTIPLRHICNSAGIMEFDDHRFE
                     MVRSGIITYGIYPSEEVKKERLDLIPALSWKSHVIHVKEVGPGIGVSYGATYVTEKPM
                     TRIATVSAGYADGYPRALSNQGCVLIHGKKAPIIGRICMDQMMVDVTDIPDVQVEDVV
                     TLVGTDGDETITIEEIANPAARFDYEMLCDISSRVTRVYK"
     gene            4002..4856
                     /locus_tag="PLN77_06495"
     CDS             4002..4856
                     /locus_tag="PLN77_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM43960.1"
                     /translation="MKKKMVSILTAAFCASALLAGCGSADTAKTSASSAGAASSSADG
                     TTTFTVGFDAEYPPYGYMDDETGDYTGFDLELAEAVCEIYGWKLVKTPINWDAKDTEL
                     NSGAIDCIWNGFTMNGREDDYTWSDPYVDNSQVMVVSENSGINSLSDLAGKTVGVQAA
                     SAALDLLQSEEEGGQKELADTFAALQQFPDYNNAFVELQAGSIDAVAMDIGVAKYQLE
                     SRGEGYKILDEHLNSEKYAIGFKKGNTELCDKVNEGLQQVLADGTFDKLAEKYGIADM
                     VCLEKKGE"
     gene            4863..5510
                     /locus_tag="PLN77_06500"
     CDS             4863..5510
                     /locus_tag="PLN77_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM43961.1"
                     /translation="MKLSMILSQLSTGMAGTISIFVLTLVFSLPLGLLIAFGRMSKNR
                     IVSGIFRLYISLMRGTPLMLQLMVVYFGPFYLFGMNIGKWRFPAIITGFALNYGAYFA
                     EIYRSGIVSMDRGQYEAAKLLGYSKGQTFVRIILPQVVKRILPAITNEVITLVKDTSL
                     AFSISYVEMFTIAKQIAAAKTTMVPFVAAGIFYYIFNFVVAWVMELLEKKFSYYH"
     gene            5535..6293
                     /locus_tag="PLN77_06505"
     CDS             5535..6293
                     /locus_tag="PLN77_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM43962.1"
                     /translation="MIYLEMNHVRKSFEDLEVLKDISLKVEEGEVLSIIGPSGSGKST
                     LLRCAAMLEMMDGGDLTYLGEQAVTMQNGKSVYAKPAVLRKIQENFGMVFQSFHLFPH
                     YDVMKNLCDAPIHVQKRNRAEVEAQAEELLQKMGLTGKEKAYPCQLSGGQCQRVAIAR
                     ALMLNPKVLFFDEPTSALDPELTGEVLKVIKSLAEMNMTMVIVTHEMNFAREISNRVI
                     FMDQGVIAVEGTPDEVFDSENARMQGFLGKFREK"
     gene            6461..7897
                     /gene="purB"
                     /locus_tag="PLN77_06510"
     CDS             6461..7897
                     /gene="purB"
                     /locus_tag="PLN77_06510"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRM43963.1"
                     /translation="MSGTDRYVSPLSERYASKEMQYIFSPDMKFRTWRKLWIALAETE
                     KELGLSITDEQIEELKANAENINYDVAKEREKVCRHDVMSHVYAYGVQCPKAKGIIHL
                     GATSCYVGDNTDIIVMNEALKLVKKKLVNVIAELAKFADTYKDQPTLAFTHFQPAQPT
                     TVGKRATLWMQEFLMDLEDLEYVQSTLKLLGSKGTTGTQASFLELFDGDQETIDKIDP
                     MIAEKMGFKNCYPVSGQTYSRKVDTRVLNVLAGIAASATKFSNDIRLLQHLKEVEEPF
                     EKGQIGSSAMAYKRNPMRSERIASLSRYVITDALNPAITSSVQWFERTLDDSANKRLS
                     IPEGFLAIDGILDLCMNVVDGLVVYPKVILKHMMAELPFMATENIMMDAVKAGGDRQE
                     LHERIRELSMIAGKHVKEEGRDNDLLDLIAADEMFHLTKEELEKTMDPSKYTGRASVQ
                     VDAFLKNVVNPVLEANKEALGMTAEINV"
     gene            8039..>8201
                     /locus_tag="PLN77_06515"
     CDS             8039..>8201
                     /locus_tag="PLN77_06515"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRM43964.1"
                     /translation="MIKAVVGANWGDEGKGKITDMLAKEADIVVRFQGGANAGHTIVN
                     NYGKFALHTL"
BASE COUNT         2333 a         1835 c         2128 g         1905 t
ORIGIN      
        1 gtatcggttt ttgtgccgat acgttttttc ttgcatttcc aatattacgc tgtttatttt
       61 acagagttaa tcagttcttc cagctcctca taggaaacgg caccgacgtt tttctttacg
      121 agctcgccgt ttttgaagaa agcgagggtc ggaacgctca tgactttaaa ggatcttgcc
      181 agctccggtg cttcgtcaac gtctactttg tagaactgga tctccggcat atcctcagct
      241 gcctcttcga tgaccggggc gagcattttg cacggtccgc accaggtagc aaagaaatca
      301 acaacggcaa cgccaggtgc tgcctgcaga gctttttcaa agttttcgga tgtaacatga
      361 attaatgaca taatagaagt cctccttttt taaaacggga ttttttattt cccggtaact
      421 gtttttttgt tgtgactgta gaatatcaca gcagaacggg aagttctgtg actaagtcac
      481 aaaatgattt cttatttttt atttcggttc ttttccctgg cggaagcatt cgatcatttc
      541 accggtcaga ctgatgaggt tcggcgtctc cggaagagaa tcgcggtcaa accattcggc
      601 aacggagagt tcctcgcggt cgagggtaat ggacggatcg ccgtccacat cgcagtaaaa
      661 tccggcgagc agcgagccgc tgaaggacca cggctgactc ttatagtatc tcagattttt
      721 gacctttaat ccgacttctt ccatgacttc acggtgcacc gtctgttcca gcgtctcacc
      781 gacctcggta tagccggcca cgagggcgta ctggcggtgg ctgctgtggg aaggtgcgta
      841 cttcgtgagc agcaggcggt caccgtcggt gatggcaacg atgacgctcg ggcagatgac
      901 cggataggag agtgcgccgc aggaagggca ctgcatcgcg cgttcatttc caggcgcatg
      961 catcttcgtt ccgcagcggc cgcagtaggt gtggtcggtg taccaggtgt ggatctggta
     1021 accggtgata ccggcaaacc cgagccatcc cggaacggcg ctgcgaagaa ttagtgtatt
     1081 ttcgtatttc cagatgccaa attccggaag ctgcggactg cgcagttcat aatagtccgt
     1141 ctcatcaatg cggaacagaa attttgcctt ttctttaatc tccggaaaaa cagctgcaat
     1201 ttccgcaatg gaagggtagc agatctcggg atcttcgaaa ttgcacagaa ttgtatgatg
     1261 accgtaaatt aaaacaagat ccgtatcgtt cggaacagaa ggctggtaat ctacataata
     1321 tttatgcgga gcaatgtcct gaatcataag aattcctttc tttggtaaaa aagtttttgg
     1381 aaaaacaaaa aaccgaaatt cctgtgaaga aatttcggta ataaaaagag cgcgagacgg
     1441 gattcgaacc cgcgacccca accttggcaa ggttgtactc cacccctgag ccactcgcgc
     1501 atattcatcc tttaggatga agcgggtgat gggaatcgaa cccacgtatc cagcttggaa
     1561 ggctggtgtt ctaccattga actacacccg cagatgattg gcactgattt tttttctctg
     1621 tcttgttgac tgccgtgcct cactgacaaa acgtattcta caggaaagcc ggagaaaagt
     1681 caaccccttt tttaaaaaaa attgaaaaaa attttaaaat actgaaaaag gtgaaaattc
     1741 agggcaaatg gcgcgagtga acgtccggtg aatagcacgt aaatatcagt cttgttccgg
     1801 gaaggaaagt gtactataat aaaaaaacga aaaacaaaga aaaaggggga acttccctat
     1861 gcaggagaat ggagcagcac ctcgggatcg gcattctgat gcaggatatt gcagaaacct
     1921 tcgaagaata gaaaaaccgg catctgtgca atttaaagaa aaattctaat ctttctataa
     1981 aatattttgg gaaaaactgg acttatctga aaaatttgat ataatagaaa ggtagtgaca
     2041 gggaaatttt atgctaaaat ctggaaagaa gaaccctgtc aattcttatg ggaggaaaaa
     2101 tacatggatg ccattgatta taagattctg aagatattgc agaaaaatgc ccgcgagacg
     2161 gcttccaata tcagcaaaga gattcattta tcggtttctg cagtcatcga gagaatccgt
     2221 aaaatggaag aaaccggagt gattaaagaa tacaccatta ttgtagacga aaagaaaacg
     2281 ggaaatgaga tgaccgcgct gatggaagtc agcctggagc atccgaagtt ctttgacagt
     2341 tttgcagatg cgatccagaa actggagtac atcgtatcct gctattatca gaccggagat
     2401 tttgatttga ttatccgcat ttcctgtcac tcatccgacg aactcgaaga aatccaccgc
     2461 caggtgatga gccttccggg cgtcgatgcg acaagaacgc atgtagtgct gaaaaatgta
     2521 aaaaatatct attccgccat ccgcagaccg gacaaataaa aaccggcaca gccggaatct
     2581 gatacgagag cgtgtctgaa aataatccga aagagttttc aggcacgttt tggcgtctca
     2641 gaaaaagtac ggaaagaaag aaggaaaaaa gtgaacagat acttacgctg ttatgcagag
     2701 atttcactcg aagcgatcgg gcataacatc agagaagtga aaaaaagact accggagggc
     2761 gtgaaactcc ttggcgttgt aaaagcgaac gcctacggcc acggtgccgt tccggtagca
     2821 tcctatctgg aaaaccaggt tgattatttt gcaaccgcaa ccatcgagga ggctgtcgag
     2881 ctcagagaaa acggcatttc cgcgccgatc ctgattctcg gctacgtgtc cccgtcccag
     2941 tacggcgacc ttgtggaata cgacatcact cagacgatcg acagctacgc acaggcgctt
     3001 gccctcgaaa aagaggcagc acgccagaac cggaaagcaa aagcacacct tgcggtcgat
     3061 accggcatga cgcggatcgg cttccaggtg acggagcacg atgcggatga ggcggcaaaa
     3121 atcgcggatc ttccgcacat cgagctggaa ggtatgttta cgcatttctc ctgcgccgat
     3181 caggaagaca agacgtactg ttcgatgcag atggaaaaat atgataagat gacggcactt
     3241 ttagcagagc gcggcgtgac gattccgctt cgccatatct gcaactccgc cggaatcatg
     3301 gaatttgacg atcaccgctt tgagatggtg cgttccggta tcatcaccta cggcatttac
     3361 ccatccgagg aggtaaaaaa agagcgcctg gatctgatcc cggcgctttc ctggaagagc
     3421 catgtcatcc atgtgaagga agtcggcccg ggcatcggcg tcagctacgg tgccacgtat
     3481 gtgaccgaga aaccgatgac acggatcgca actgtttcag ccggctatgc agacggctat
     3541 ccgagagctc tctcgaacca gggctgtgtg ctgatccacg ggaaaaaagc accgatcatc
     3601 ggaagaatct gtatggatca gatgatggtg gacgtgaccg acatcccgga tgtgcaggtg
     3661 gaggacgttg taacgcttgt cggaaccgac ggggatgaga ccatcacgat cgaagagatc
     3721 gccaacccgg cggcacgttt cgattacgag atgctctgcg atatcagcag ccgtgtaaca
     3781 agggtttata aatagttatc caaaatagaa aaatcaaagt ctccgaagac ggaaaaaagc
     3841 agaatctgct gaccgtttcg gagacttttt tgtgttggaa caggttttct ggattattta
     3901 accggtgcgg gttgacagga ggtgccggag tatggtagga taccaattag tttagcgtag
     3961 tacagtgata acatgatcac gtaaaaaagg agaagattat tatgaaaaag aaaatggtta
     4021 gcattttgac agcagccttt tgtgcatctg ccctgctggc tggctgcggc tctgcagaca
     4081 cagcaaaaac atctgcctca tcagcaggag cagcatcctc ttccgcagac ggaacgacaa
     4141 cctttaccgt aggcttcgat gcagaatacc cgccgtacgg ctacatggac gatgagaccg
     4201 gcgattacac cggatttgac ctggagctcg ccgaggcagt ctgcgagatt tacggctgga
     4261 agctggtaaa aaccccgatc aactgggatg cgaaagatac cgagctgaat tctggtgcga
     4321 ttgactgcat ctggaacggc tttaccatga acggcagaga ggacgattac acctggtccg
     4381 atccgtacgt ggacaacagc caggtgatgg tcgtatctga gaattccggc atcaacagcc
     4441 tgtccgatct ggcaggaaag acggttggtg tgcaggcagc atccgcagca ctcgatcttt
     4501 tacagagtga ggaagaaggc ggtcagaaag aactcgccga tactttcgcc gctctgcagc
     4561 agttcccgga ttacaacaat gcgtttgtag agcttcaggc aggttccatc gatgcggttg
     4621 ccatggatat cggcgttgcc aaatatcagc tggaaagccg cggagaagga tacaaaatcc
     4681 tcgacgagca cctgaactct gagaaatatg cgatcggatt caaaaaagga aataccgagc
     4741 tctgcgataa agtaaacgaa ggcctgcagc aggttctggc cgacggaaca ttcgacaagc
     4801 tcgcagaaaa atacggaatc gcagacatgg tgtgtctgga gaaaaaagga gagtaaagca
     4861 gcatgaagtt gtccatgatt ctatcacagc ttagtaccgg aatggcggga acgatctcga
     4921 tatttgtcct gacgctcgta ttttccctgc cgctcggtct gctgatcgcc ttcgggcgta
     4981 tgtcgaaaaa ccggattgtg agcggaattt tccggctcta tatctccctg atgcgcggca
     5041 cgcctctgat gctgcagctc atggtggttt acttcggacc gttctatctg ttcggtatga
     5101 acatcggaaa atggcgcttt ccggcgatta tcaccggatt tgccttaaac tacggcgcct
     5161 attttgcaga aatctaccgc tccggcatcg tttcgatgga tcgtggacag tacgaggcag
     5221 caaagcttct tggttattcg aagggacaga cgtttgtccg cattattctg ccgcaggttg
     5281 tgaaacggat tctgccggcg atcaccaatg aggtgattac gctggtcaag gatacgtcgc
     5341 tggccttttc catttcctat gtggaaatgt tcacgatcgc aaaacagatc gcggcggcca
     5401 agaccacgat ggttccgttc gtcgccgccg gtattttcta ctacatcttc aattttgtcg
     5461 tggcgtgggt gatggaactt ttggaaaaga aattttctta ttatcattaa ctatagaaga
     5521 gagcggaaaa cgatatgatt tatctggaaa tgaaccatgt aagaaagagc tttgaggatc
     5581 tcgaagtgtt aaaagatatt tccctcaaag tagaagaggg cgaggtgctc tcgatcatcg
     5641 gcccgtcggg atcgggaaaa tcgacgctgc tgcggtgtgc ggcgatgctg gagatgatgg
     5701 atggcggtga cctgacgtat cttggcgagc aggcagtcac catgcaaaat ggaaaatccg
     5761 tttatgcaaa gccggccgtg ctgcggaaaa ttcaggaaaa ctttggaatg gtattccaga
     5821 gcttccacct gtttccacat tatgatgtga tgaaaaacct ctgcgatgcg ccgattcatg
     5881 tgcagaaacg caaccgtgcg gaggtggaag cacaggcaga agagctgctg cagaaaatgg
     5941 gactcacagg aaaagaaaag gcgtatccgt gtcagctttc cggcggtcag tgtcagaggg
     6001 ttgcgattgc gcgtgcgctg atgctgaacc cgaaggtgct cttttttgat gagccgactt
     6061 cggcgctcga cccggagctg acaggcgagg tgctgaaggt catcaaatcc cttgcggaga
     6121 tgaatatgac catggtgatc gttacccacg agatgaattt tgcccgggag atttccaacc
     6181 gcgtcatctt tatggaccag ggcgtgatcg ccgtggaagg aacgccggac gaagtcttcg
     6241 attccgaaaa tgccagaatg cagggctttt tgggaaaatt tcgtgagaag taacgacgtt
     6301 gcacttgcct aaaaaaacaa catgcgttat actagagcgt ggttgaaacg tatttcaaac
     6361 acgctcttta tacgtcccgt tctgtttttg gggatgaaaa agagcgggga taaaagagaa
     6421 accgaataca cattttgaca agaaaaggag aatcgtgtct atgagcggaa ctgacagata
     6481 tgtaagccct ctttccgaac gttatgcaag caaggagatg cagtacatct tctccccgga
     6541 tatgaaattc cgtacctgga gaaagttatg gattgcgctg gctgagacag aaaaagaact
     6601 gggactttct attacagatg agcagatcga agaactgaaa gcaaatgccg agaacatcaa
     6661 ctacgatgtg gcaaaagaaa gagagaaagt ctgccgtcat gacgtaatgt cccatgtgta
     6721 tgcctacggc gtacagtgtc cgaaagcaaa aggaatcatc catctgggag caaccagctg
     6781 ctacgtaggc gacaataccg atatcattgt catgaacgaa gccttaaagc tggtgaagaa
     6841 aaaactggtg aacgttatcg ctgagctggc aaaatttgcg gatacctaca aagaccagcc
     6901 gacccttgcc ttcacccact tccagccggc acagccgacc accgtcggaa aacgtgcaac
     6961 cctgtggatg caggagttct taatggacct tgaagatctc gagtacgtac agagcacctt
     7021 aaagctgctc ggctccaaag gaacaacagg cacacaggca agctttttag agctgttcga
     7081 cggagatcag gagaccatcg acaagatcga tccgatgatc gctgagaaaa tgggcttcaa
     7141 gaactgctat cctgtttccg gacagacgta ttccagaaaa gtcgatacga gagtattaaa
     7201 cgttctggca ggcattgcgg caagcgcaac aaaattctcc aacgacatcc gtcttctgca
     7261 gcacttaaaa gaagttgagg agccgttcga gaaaggacag atcggatctt ccgccatggc
     7321 atacaagaga aatccgatga gaagcgagcg tatcgcatcc ctgtcccgtt atgtgatcac
     7381 cgatgcatta aatccggcca tcacctcttc tgtacagtgg tttgagagaa ccctcgatga
     7441 ctctgcaaac aagagacttt ccattccgga aggcttcctt gccatcgacg gtatcctgga
     7501 tctgtgcatg aacgtcgtag acggacttgt ggtttatcca aaggttatct taaaacacat
     7561 gatggcagag cttccgttta tggcaaccga gaatatcatg atggatgcgg tgaaagcagg
     7621 cggcgaccgt caggagctgc atgagagaat ccgcgagctg tccatgatcg ccggaaaaca
     7681 tgtcaaagag gaaggccgcg ataatgatct tctggatctg atcgcagcgg atgagatgtt
     7741 ccatctgacc aaagaagagc tggaaaagac gatggatccg tccaaataca caggccgcgc
     7801 atccgtacag gttgacgcat tcctgaagaa cgtagtcaat ccggtgcttg aggcaaacaa
     7861 agaggcactt ggcatgactg cggaaatcaa cgtctgatgc gtttgataaa cagaaataat
     7921 aaaaaaacag attttataag taatcataag tttcgcttat gagaaaaaca tggtatacta
     7981 aagataaacg gccggaacaa aaaggccgca agtatacgtt gaattaggag gatgtcatat
     8041 gattaaggca gtagtaggag caaactgggg agacgaggga aaaggtaaaa ttaccgatat
     8101 gctcgcaaaa gaggcagaca ttgttgtccg tttccagggt ggtgcaaatg caggccatac
     8161 cattgtaaac aactatggaa agtttgcact tcataccctt c
//
LOCUS       DAOMUF010000093         8096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2310_length_8096_cov_3.977615, whole genome shotgun sequence.
ACCESSION   DAOMUF010000093 DAOMUF010000000
VERSION     DAOMUF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_8096_cov_3.977615"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..208
                     /locus_tag="PLN77_06520"
     CDS             17..208
                     /locus_tag="PLN77_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HRM43965.1"
                     /translation="MAEHYEVGDIVKLKKPHPCGSSEWEILRVGADFRLKCTGCGHQI
                     MVARKLVEKNTRGLRKKDA"
     gene            356..643
                     /gene="rpsF"
                     /locus_tag="PLN77_06525"
     CDS             356..643
                     /gene="rpsF"
                     /locus_tag="PLN77_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRM43966.1"
                     /translation="MNKYELALVVNAKLEEEERAAVVEKAKGYVTRYGGTVGEVEEWG
                     KKRLAYEIQHMREAYYYFIQFEADATAPAEIESHMRIMDNVLRYLVVKKEA"
     gene            655..1116
                     /gene="ssb"
                     /locus_tag="PLN77_06530"
     CDS             655..1116
                     /gene="ssb"
                     /locus_tag="PLN77_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM43967.1"
                     /translation="MNKVILMGRLTRDPDVRYSAGENSTAVARYTLAVDRRFRRDGDS
                     ATADFIGCVAFGRQAEFAEKYLRQGTKIAITGRIQTGSYTNREGQKVYTTDVVVEEQE
                     FAESKNAGGNNGGYSAPQHNNPAPSANTSDLGSADGFMNIPDGIDEELPFN"
     gene            1137..1385
                     /gene="rpsR"
                     /locus_tag="PLN77_06535"
     CDS             1137..1385
                     /gene="rpsR"
                     /locus_tag="PLN77_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRM43968.1"
                     /translation="MAYNNSERPASRRPARRRRKVCVFCGGEEISYKDAAKLKKYVSE
                     RGKILPRRVTGTCAKHQRAVTVAVKRARHMAIMPYVDD"
     gene            1575..3620
                     /locus_tag="PLN77_06540"
     CDS             1575..3620
                     /locus_tag="PLN77_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRM43969.1"
                     /translation="MKSKMKLSGQLRSYLRWPLILSILMLVMNIGMYFVDVMAGMWFT
                     LFFAVYVIAAAVLYYRQRPQVINELITFATEYGQVQKHLIQELALPYAVMDSQGKLLW
                     VNKEFTKITGKDTQYHKCIATIFPELTVERLPKNEEETDINVAYGEKNFRAHVKSVCI
                     DELIQNSEMINTDIKGYFIQALYLFDETELREYMRKFEDETMVTGLLYLDNYDEALES
                     VEEVRRSLLTALVERKINKYFNDLDGLVKRYENDKYIVVMRRSSLNELKEKKFDILED
                     VKTINIGNEMAVTISMGIGADAGSFAKNSEYAKIAIDLALGRGGDQVVLKDGSKIQYF
                     GGKTQAVEKNTRVKARVKAHALKEFMNTKEKVVVMGHRLPDADSFGAAIGIYRAAKTL
                     NKKAYIVIDNPTSSIIPLMNTFRDNQDYEADMFVNNHEAKEIMDDNTLLVVVDTNKPS
                     ITQCEDLLYMAKMIVVLDHHRQGSDTIRNSVLSYIEPYASSASEMVAEILQYFTEGIR
                     IRNVEADSIYAGIMIDTDNFMQKTGVRTFEAAAFLRRCGADVTRVRKLFREDMNDYKA
                     RGETIRNAELFRGQFAISECPSDYVDSPTVVGAQAANELLNIVGVKASFVLTEYKGVI
                     YISARAIDEVNVQIIMERMGGGGHLNMAGCQLETVNMEGARNLLKSTLTEMQDGGEI"
     gene            3622..4068
                     /gene="rplI"
                     /locus_tag="PLN77_06545"
     CDS             3622..4068
                     /gene="rplI"
                     /locus_tag="PLN77_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRM43970.1"
                     /translation="MKVILLEDVKALGKKGQTVNVSDGYARNMLLPKKLGIEATAKNL
                     NELKLQKAHEDKVAAEQLAEAQAFAKEIEDKQVTVAIKVGEGGKVFGSVSAKEIAEAA
                     KEQLGYDIDKKKMQLPNPIKVLGTTEVPIRLHTKVTAKLKVIVKEA"
     gene            4073..5407
                     /gene="dnaB"
                     /locus_tag="PLN77_06550"
     CDS             4073..5407
                     /gene="dnaB"
                     /locus_tag="PLN77_06550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRM43971.1"
                     /translation="MEEALIKRILPHSIEAEQSVIGSMLMGREAIMAASEILTSDDFY
                     QHQYGVIFDAMVELFNEGKPVDLVTLQNRLREKDVPPEISSMEFVRDLLNAVPTSANV
                     RHYANIVSEKAVLRRLIKLTEEIENDCYLNKEPVEVIMEETEKKMFQLLGQRNSGDFM
                     PIRQVVINTLENIERASKTKGNVTGIPTGFTDLDYKTSGLQNSDFILIAARPSMGKTA
                     FVLNIAQYMAFKKDKAVAIFSLEMSREQLMNRLLSMESKVDSQHLRTGNLKDDEWSKL
                     IESAGMIGESRLMIDDTPGISIGEMRSKCRKYKLEHGLDIIIIDYLQLMSGSGKSSSE
                     SRQQEISDISRSLKALARELNVPVVALSQLSRAVEQRTDHRPMLSDLRESGAIEQDAD
                     VVMFIYRDDYYNKDSENKGIAEIIIAKQRNGPIGTVNLVWLPDYTKFANAER"
     gene            5565..6161
                     /locus_tag="PLN77_06555"
     CDS             5565..6161
                     /locus_tag="PLN77_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM43972.1"
                     /translation="MENRTYLVTEAEKLTGEKAHVLRYWEDELGMEIHRNSLGHRCYT
                     AQDIQIFLGIRELKKKGLHLKAIRELMPKLVQATAEIKAFPAEAAETAKEKPDPAIPG
                     KADEVPEVVVDLLPEREAEQLADTVMRQEEEERQTEFYRVLGRLLDQMIQKRRQEERS
                     RRVDEAIRRHQQSRREAAATLEQKGKRTKAKRGHRNNG"
     gene            complement(6225..6428)
                     /locus_tag="PLN77_06560"
     CDS             complement(6225..6428)
                     /locus_tag="PLN77_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRM43973.1"
                     /translation="MAKISKDMTIGELIAMDTNIIGILMGAGMHCIGCPSAQGESLEE
                     AAMVHGLDAQVLEDQINDYLAAK"
     gene            6635..6805
                     /locus_tag="PLN77_06565"
     CDS             6635..6805
                     /locus_tag="PLN77_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM43974.1"
                     /translation="MAYVITDECVSCGTCAGECPVEAIAEGDSHYEINPDVCVECGTC
                     ASVCPTEAITEE"
     gene            complement(6874..7623)
                     /locus_tag="PLN77_06570"
     CDS             complement(6874..7623)
                     /locus_tag="PLN77_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HRM43975.1"
                     /translation="MKIEKINDNKIRCTLTSADLAERNLKLSELAYGTEKARSLFQDM
                     MLEANQQFGFNVDNAPLMVEAVPVAPDSIVLIITRVEDPQELDTRFAKFAPSGDDTDG
                     PEPVQYSGADDIIDLFHKLCDVKNKAQEIAKKAAQKENEKEPAASEEKNVDLVRTFHF
                     STLDDVISSAKALNQYFTGSNSLYKNRADANYQLVLHQSGCKPEEFNKVCNILSEYGR
                     QESFSLAAEAFLLEHGNAMVKENALQELASL"
     gene            complement(7670..>8096)
                     /locus_tag="PLN77_06575"
     CDS             complement(7670..>8096)
                     /locus_tag="PLN77_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43976.1"
                     /translation="ESESIVTVHWFPANCNIRFLLLGIFQKICLKSFVDCLFHGLFAV
                     VTYKKESPIDPYEILLFLVSMIMFRGDAFLSDPFFLCLLPAGVCTLRTEAKVSPTPII
                     LQTGGITNKVYPFISEKIMRNVKKILSSLLLSVMCYTIS"
BASE COUNT         2301 a         1778 c         2222 g         1795 t
ORIGIN      
        1 ataaggcgga agaaacatgg cagaacatta cgaagtaggc gatattgtaa aactgaaaaa
       61 gccgcatccg tgcggcagca gcgagtggga aattctgcgt gtcggcgcag atttccgtct
      121 caaatgcacc ggctgcggcc accagatcat ggtagcacgc aagctggtcg agaaaaacac
      181 ccgcggatta cgaaaaaaag atgcgtaata tcgtgaaaaa aacaagaaaa cacgcgaaaa
      241 agggcttgct ataagccctt ttttatgtta aaataacaaa ttgtgacgta taaaaataat
      301 tccttgctcc ccataatgaa aatggggtgc caaaaagtcc aaaaggaggt aaagcatgaa
      361 caagtacgaa ttagcactgg ttgtgaatgc aaaacttgaa gaagaggaac gcgcagctgt
      421 agttgagaaa gccaaaggtt atgtaacccg ttacggcgga acagttggcg aagtagaaga
      481 gtggggcaaa aaacgccttg catacgaaat tcagcatatg cgtgaagcat attactactt
      541 tattcagttt gaagcagacg caacagctcc ggcagagatc gagagccata tgcgtattat
      601 ggataacgta ctgagatact tagtcgttaa aaaggaagct taagaggtaa gcgaatgaat
      661 aaagttatat tgatgggaag attgaccaga gatcccgatg tcagatactc tgctggagag
      721 aactccacag cggtagcccg ttatacactg gctgttgacc gcagattccg cagagacggc
      781 gattccgcaa ccgcagactt tatcggatgc gttgctttcg gccgccaggc agaatttgca
      841 gagaaatacc tgcgtcaggg aacgaagatc gccatcaccg gccgcattca gaccggaagc
      901 tacaccaacc gtgaaggcca gaaggtctac acgacagacg tagttgtaga agagcaggag
      961 tttgcagaga gcaaaaatgc aggcggaaac aacggaggat actccgcacc gcagcataac
     1021 aatccggctc cgtccgcgaa tacatctgac ctgggcagcg ccgatggttt tatgaatatt
     1081 cccgatggca ttgacgaaga attgccattt aattgataga aaaggagaga ttgatcatgg
     1141 cttataataa tagtgaaaga ccagcttcca gaagaccggc ccgcagaaga agaaaagttt
     1201 gtgttttctg tggtggagaa gagatcagct acaaggatgc agctaaactg aaaaaatatg
     1261 tatccgagcg tggtaaaatc ctgccgagac gtgtaactgg aacctgcgca aaacatcaga
     1321 gagcagtaac agtagccgtt aagagagcaa gacatatggc tattatgccg tacgtagatg
     1381 attgattctg acttgcgcta agcactgtcc gaacgttcat tttgaatcgg taaataaatg
     1441 gaggtatctg cccgaaaagg gcgggtacct tttttgtatc cagaagtatc ttgttgccaa
     1501 agtacaggtc aaagtggtat aatcggaagg acaggagcga aatacaacac gcaacgttgg
     1561 aaaggtgtta ggaaatgaaa agcaaaatga agttgagcgg ccaattgcgg agctatcttc
     1621 gatggccgct gattttgtcc atcctgatgc ttgtgatgaa tatcggcatg tattttgtgg
     1681 atgtgatggc aggaatgtgg ttcacgctgt tttttgcagt ctatgtgatc gcagccgcag
     1741 ttctctatta cagacagcgt ccgcaggtga tcaacgagct gattaccttt gcgaccgaat
     1801 acggccaggt tcagaagcat ctgatccagg aactggcgct gccctatgcc gttatggatt
     1861 cgcagggaaa acttctctgg gtcaacaaag agtttacaaa aatcacaggg aaggatacac
     1921 agtatcataa atgtatcgcc accatttttc cggagctgac ggtcgagcgg ctgccgaaaa
     1981 atgaggagga gaccgacatc aacgtggctt acggcgaaaa gaatttccgt gcccatgtaa
     2041 aaagcgtctg catcgacgag ctgatccaga attcggagat gatcaacaca gatatcaaag
     2101 gctattttat tcaggcactg tatctgttcg atgagacgga gctccgcgag tatatgcgga
     2161 aattcgagga cgaaacgatg gtcaccggtc ttctgtatct ggacaactac gacgaggcgc
     2221 tggagagcgt cgaggaggtc cgccgttccc ttctgaccgc actggtcgag cgaaagatca
     2281 acaaatattt caacgatctc gacggactgg tgaagcggta tgagaacgac aaatacatcg
     2341 tggtcatgcg ccggagttcg ttaaatgaac tgaaagagaa aaagttcgat atcctcgagg
     2401 acgtcaagac catcaacatc ggaaacgaga tggcggtcac aatcagtatg ggtatcggag
     2461 cagatgctgg atcgtttgcg aaaaactccg agtatgcgaa aatcgcgatc gacctggctc
     2521 ttggccgggg cggcgatcag gtcgttttaa aggacggttc gaagattcag tatttcggcg
     2581 gaaaaacgca ggctgtggaa aagaatacga gagtcaaagc ccgcgtcaag gcgcacgcgc
     2641 tgaaagagtt tatgaacacg aaagagaagg tcgttgtcat gggacaccgc ctgccggatg
     2701 ccgattcgtt cggtgcggcc atcggtatct accgtgcggc gaaaacgctg aataaaaaag
     2761 cgtacatcgt catcgacaac ccgaccagct cgatcattcc gctgatgaat acgttccgcg
     2821 acaaccagga ttatgaggcg gacatgtttg tcaacaacca tgaggcgaaa gagatcatgg
     2881 acgacaacac gctgctcgtt gtggtcgata cgaacaagcc gagcatcaca cagtgtgagg
     2941 atctgctgta catggcgaaa atgatcgtcg tgctggatca tcaccggcag ggaagcgata
     3001 cgatccgcaa ctcggtgctt tcctatattg agccgtatgc atcatccgcg agcgagatgg
     3061 ttgccgagat tctccagtat tttaccgagg gaatccgcat ccggaacgtg gaggcggaca
     3121 gcatctatgc cggcatcatg atcgataccg acaactttat gcagaaaaca ggtgtccgca
     3181 cgttcgaggc ggcagcgttc ctgcgccgct gcggtgcgga tgtgacgcgt gtgcgcaagc
     3241 tgttccgtga ggatatgaac gattacaagg caagaggaga gacgatccgc aatgcggagc
     3301 tgttccgcgg ccagttcgcg atttccgaat gcccgagcga ctatgtggac agcccgacgg
     3361 tggtcggagc acaagcggcg aacgagctgc tgaacattgt cggtgtcaag gcgtcctttg
     3421 tactgaccga gtataaaggc gtgatctata tcagcgcccg cgcgatcgat gaggtcaatg
     3481 tacagatcat catggagcgg atgggcggcg gcggtcatct gaatatggcg ggatgccagc
     3541 tcgagaccgt caacatggag ggggcgagaa accttctgaa atcaacctta acagaaatgc
     3601 aggatggagg agaaatctga tatgaaagtt atcttactgg aagacgtaaa agcacttggt
     3661 aaaaaaggac agactgtaaa cgtaagtgac ggatatgcaa gaaacatgct tctgccgaaa
     3721 aaactcggca tcgaggcaac cgcaaaaaac ctgaatgagc tgaaactcca gaaagcacac
     3781 gaggacaaag tggcagcaga gcagctcgct gaggcacagg ctttcgcgaa agagattgag
     3841 gacaaacagg ttaccgtagc aatcaaagtc ggcgagggcg gaaaagtatt cggaagcgta
     3901 tcggcaaaag agatcgctga ggcagcaaaa gagcagctcg gatacgacat cgacaaaaag
     3961 aaaatgcagc ttccgaaccc gatcaaggtt cttggaacga cagaagtgcc gatccgcctg
     4021 cataccaaag taactgcaaa gctgaaagtt atcgtaaaag aagcataatt ctatggaaga
     4081 agcactgatt aaacgaatcc ttccgcacag catcgaggcg gagcagtcgg tcatcggttc
     4141 catgctgatg gggcgcgaag ccatcatggc ggcatcggaa atcctgacga gcgacgattt
     4201 ttaccagcac cagtacggcg tcatttttga tgccatggtg gagctgttca atgagggaaa
     4261 accggtcgat ctggtcaccc tgcagaaccg cctgcgtgaa aaggatgttc cgccggagat
     4321 cagttcgatg gagtttgtcc gcgatctttt aaatgcggtg ccgacttcgg cgaatgtgcg
     4381 ccattatgcg aacatcgtca gcgagaaagc ggtgctgcgc cgtctcatta agctgacaga
     4441 agagatagag aatgactgct atctgaataa agagccggtg gaagtcatca tggaggagac
     4501 ggaaaagaag atgttccagt tattgggcca gcggaattcc ggagacttca tgccgatccg
     4561 ccaggttgtc atcaatacgc tggagaacat cgagcgtgca tcgaagacga agggaaatgt
     4621 cacgggtatt ccgaccggat tcacggatct ggactacaag acatcgggtc tgcagaactc
     4681 tgactttatc ctgattgcgg cacgtccgtc tatgggtaaa acggcgtttg tcctcaatat
     4741 tgcgcagtat atggcgttta agaaagacaa agccgttgcg attttcagtc tcgagatgtc
     4801 gcgggagcag ctgatgaacc gtctgctctc gatggagtcg aaagtagatt cccagcatct
     4861 gagaaccgga aacctgaaag acgacgaatg gagcaagctg atcgagagcg ccggcatgat
     4921 cggtgaatcc cgactgatga tcgacgatac acctggtatc tcgatcggag agatgcggtc
     4981 gaaatgccgc aaatacaagc tggagcacgg tcttgatatc atcatcattg actacctgca
     5041 gctgatgtcg ggaagcggaa agagctcctc ggaatcgcgt cagcaggaga tttcggatat
     5101 cagccgttcc ttaaaagcac tcgcgagaga gctgaatgtg ccggttgttg cactgtccca
     5161 gctttcccgg gctgttgagc agcgtaccga ccaccgtccg atgctttccg acctgcgtga
     5221 gtcgggagcg atcgagcagg atgccgacgt tgttatgttt atctatcgag atgattacta
     5281 taataaggat tccgagaata agggaatcgc ggaaatcatc atcgccaaac agcgaaacgg
     5341 cccgatcgga accgtcaatc tggtctggct gccggattat acgaaatttg cgaatgcaga
     5401 gagataaaag aaaatatttt atgtgtcaga acgtgtcgtg ggatagtcct gcggcacgtt
     5461 ctttgtgtat aattgtatga aattgtcgga atattacgat gagattttct tgcggcaggg
     5521 gataacagat cctataataa agggaagaag ggagtggctg ccgtatggaa aatagaacgt
     5581 atctggtcac ggaggcggag aagctgaccg gggagaaagc gcatgtgctc cggtactggg
     5641 aggacgagct cggcatggaa atccaccgca attcgctggg acaccgctgc tatactgcgc
     5701 aggacattca gatttttctg ggaatccggg aactgaagaa aaaggggctt cacctgaaag
     5761 cgatccgcga gctgatgccg aagctggtgc aggcaacggc ggagatcaaa gcgtttccgg
     5821 cggaagcggc agagacggca aaggagaagc cggatccggc aataccggga aaagcggacg
     5881 aagtgccgga ggtggttgtc gatcttctgc ccgagcggga agcagaacag ctggcggaca
     5941 ctgtgatgcg gcaggaagag gaggagcgtc agacggagtt ttaccgcgtg ctcggccgtc
     6001 tgcttgacca gatgatacag aagagacgtc aggaggaacg gagccgccgc gtggacgagg
     6061 cgatccgccg ccaccagcag agccggagag aagcagcggc gacgctggag cagaaaggaa
     6121 aacggacgaa agcaaaacgg ggccatcgga acaatgggta aatcggacag aaaaaaagaa
     6181 cgcctgcgag aggcgttctt tttggatcca cgcggaaatc cgacttattt tgcagccaga
     6241 taatcgttga tctggtcttc cagaacctga gcgtccaggc cgtgaaccat agctgcttct
     6301 tccagggact caccctgtgc agaagggcag ccgatgcagt gcataccggc tcccatcagg
     6361 attccgatga tatttgtatc cattgcgatt aactcgccga ttgtcatgtc tttcgagatc
     6421 tttgccatct taaagttcct cctttaaatg tgtatatacc cgtcgttccg attttgacgg
     6481 ctattttgat tatagcgtac ccccgggggg aatgcaagaa caaattcata agattataga
     6541 atgtttataa ttttgtactt gtatttcagg gggcaatcgt ttacaatgtg gatagtaccg
     6601 aaagggccaa aaaatctaca aggaggataa gatcatggca tatgtaatca cagacgaatg
     6661 tgtaagctgc ggaacctgcg ctggagaatg tccagtagag gctatcgctg agggagattc
     6721 ccattacgaa atcaacccgg atgtatgcgt tgagtgcgga acatgcgcaa gcgtatgtcc
     6781 aaccgaggct atcacagaag agtaatttgt acggaacgta caaaaaagga agtccttcgc
     6841 ggacttcctt tttttataca tacgtttcaa aacttacaga gaagccagct cctgcagagc
     6901 attctctttt accattgcat ttccatgttc cagcagaaat gcttcagcgg ccagggagaa
     6961 ggattcctgt ctgccgtact cggaaagaat gttgcagacc ttgttgaatt cctccggttt
     7021 gcagccggac tggtgcagaa cgagctgata gttggcatct gccctgttct tatagaggga
     7081 attgctgccg gtaaaatact ggttcagcgc ttttgcggag ctgatgacat cgtcgagggt
     7141 tgagaagtgg aaggtgcgta ccagatccac attcttttcc tcggatgctg caggctcttt
     7201 ttcgttttcc ttctgtgccg ctttctttgc gatttcctgt gccttgttct tcacatcgca
     7261 gagcttgtgg aacaggtcaa tgatgtcgtc cgcgccggaa tactgtaccg gttccggtcc
     7321 gtccgtatcg tcgccggatg gagcaaattt ggcgaagcgg gtgtcgagct cctgtggatc
     7381 ctcgactctg gtgataatga ggacaatgct gtccggagcc accggaacgg cctcgaccat
     7441 gagcggggca ttatccacat tgaagccgaa ctgctgattg gcttcaagca tcatatcctg
     7501 aaacaggctg cgggcctttt ccgtgccgta agccagttcg ctgagcttta aatttctttc
     7561 ggcaagatcg gcgctggtca gtgtgcagcg gatcttgtta tcattgattt tttctatttt
     7621 cataactctc acgtccttaa ttactcttta cttatataaa tagggctgct tacgatatag
     7681 tataacacat cacggaaaga agtaaagaac ttaagatttt ttttacattt cgcataattt
     7741 tttcagaaat gaagggataa actttatttg taattccccc ggtttgaagt ataatgggag
     7801 ttggggaaac ctttgcttct gtccggaggg tgcatactcc ggccggaagc aggcacagaa
     7861 aaaacggatc ggataagaag gcgtccccgc gaaacatgat cattgaaact aaaaacaaaa
     7921 gtatttcata cggatcgatg gggctttctt ttttgtacgt cacaactgca aataagccat
     7981 gaaacaaaca atcgacaaaa ctttttaaac agattttctg aaaaataccc aatagcaaaa
     8041 agcgaatgtt acagttcgct gggaaccagt gaactgtaac gatactttct gactcg
//
LOCUS       DAOMUF010000094         8088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2313_length_8088_cov_4.310469, whole genome shotgun sequence.
ACCESSION   DAOMUF010000094 DAOMUF010000000
VERSION     DAOMUF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2313_length_8088_cov_4.310469"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1600
                     /locus_tag="PLN77_06580"
     CDS             386..1600
                     /locus_tag="PLN77_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM43977.1"
                     /translation="MLQEWKRKGKKFRHEEKDGEETTELSVKIRAAKFRDLPMSLRIL
                     IVNLSLFLILCAVFFMAGAYLYAPQAAGRNYVEACLAGDWNSAYDVCQFPDSTFLSRK
                     NYVNAMTWKAKQDGTDGQETPEIKSFFMRRKQNPETGENRIYTVRYTLKGMSDSQEET
                     LEIAAGDTVKWNFKEWYVVPKDSCVTDVEITVPADASFYLDGVQVGKKYLKETADNVS
                     IYKIPYLFLGGHTIELTEKQKDPYREIILVQDNSSMEFLPDLKLNDSTGKTIADRAEE
                     SLDKVFTAAAAGKSFSTIKNEFSADTAVQKTAKEQYQQLCDAYLNSDTNTGITSITVS
                     SVSTTVTNESNQMKIETNVTAAIERRTRFLHFFRKTKIETVTWKLESVVTLENEKWVM
                     GSDFLKGVELSK"
     gene            2088..3164
                     /locus_tag="PLN77_06585"
     CDS             2088..3164
                     /locus_tag="PLN77_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM43978.1"
                     /translation="MKQRRKQMRRRQMMQKVSRISLCVVIGLLFLALIWKFAGAQLAK
                     KGIVLSNDTAAKTVSETAPEAEDGSGNAGIKKLAETTPPNETPGWQYSSSGWWYATDA
                     TTYYVNGWADIDGNQYHFDSNGYMATGWKPIGGKGCYFDDQGVYQPDRNGSMMVALTF
                     DDGPGVYTSTLLDPLEQYGAQATFFMLGSNVEKYGADVIPRMAAIGCELGNHSYAHPN
                     MKELSLDDGLAQFQMTDDLIAQYNNGQGASVVRFPYGNSTDELLASIGRPSIMWDVDT
                     LDWQTKNVQANISAVLDSVSPGDIILMHDIHETTVESCATIVPELINRGYELVTIHTL
                     AAANGVDLAAGETYYGFHGGTTNE"
     gene            complement(3252..5081)
                     /gene="typA"
                     /locus_tag="PLN77_06590"
     CDS             complement(3252..5081)
                     /gene="typA"
                     /locus_tag="PLN77_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRM43979.1"
                     /translation="MKTTREDVRNVAIIAHVDHGKTTLVDELLKQSGVFRANQEVQER
                     VMDSNDIERERGITILSKNTAVYYKGTNGDSTLTLDIVRVHGEVERVLKMVDGVILVV
                     DAFEGAMPQTKFVLKKALELDLSVIVCINKIDRPEARPDEVIDEVLELLMDLDASDEQ
                     LDCPFLYASAKMGHAVLDLNDTPKDMTPLFETILKYIPAPTGDPEAGTQLLISTIDYN
                     EYVGRIGVGKVENGTIRVNQDVMLVNHHDLTKKKKVKVSKLYEFDGLKKVEVTEAGIG
                     SIVAVSGIPDIHIGDTLCGDLDNPVSIPFQKISEPTISMNFIVNDSPLAGKEGKYVTS
                     RHLRDRLMRELNTDVSLRVEETDSADCFKVSGRGELHLSVLIENMRREGYEFAVSKAE
                     VIYKEDERGRKLEPMEIAYVDVPDEFSGTVIQLLSERKGELHGMSRASDGSTRLEFEI
                     PARGLIGFRGIFMTSTKGTGILNTEFEDYAPYKGDIEYRKQGSLIAFEAGESVTYGLY
                     SAQERGTLFIGPGEKVYAGMVIGQNGKAEDIELNVCKTKHLTNTRTSSSDEALRLTPP
                     RILSLEQALDFIDKDELLEVTPVSLRIRKKELDSRLRKRAAMK"
     gene            5487..6116
                     /locus_tag="PLN77_06595"
     CDS             5487..6116
                     /locus_tag="PLN77_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM43980.1"
                     /translation="MADKMIENNQVVIMGEIVSPFTFSHQVFGEGFYLVDVMVKRLSE
                     SQDIIPVMVSERLVDVSQDYQGEWICISGQFRSYNRHEEKKNRLVLSVFARELSFVEE
                     EDDSVKSNQIYLDGYICKPPVYRKTPLGREIADLLIAVNRPYGKSDYIPCICWGRNAR
                     YASAFAVGGHVLIWGRIQSREYMKRLSETEMEKRTAYEVSVSKLEYSES"
     regulatory      6172..6346
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            6498..7388
                     /gene="dapA"
                     /locus_tag="PLN77_06600"
     CDS             6498..7388
                     /gene="dapA"
                     /locus_tag="PLN77_06600"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRM43981.1"
                     /translation="MSIFKGAAVAIITPMTETQEVNYEKLGELIEEQIAGGTDAIVIC
                     GTTGEASTLSHEEHLDAIKYTVEKVNHRIPVIAGTGSNSTETAIYLSQEAEKYGVDGL
                     LLVSPYYNKATQKGLIAHFTAIADSVKVPVILYNIQGRTGVNIAPETIVYLFNNVENI
                     VAVKEASGNISQVAKIMQLTDGKIDLYSGNDDQVVPILSLGGSGVISVLSNVAPRETH
                     DMVQKFLDGDVAGSREIQLRALPLIDALFCEVNPIPVKAALNLMGKEVGPLRLPLTEM
                     EPQNQERLAKAMKDFGIKLA"
     gene            7424..>8088
                     /gene="dapB"
                     /locus_tag="PLN77_06605"
     CDS             7424..>8088
                     /gene="dapB"
                     /locus_tag="PLN77_06605"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRM43982.1"
                     /translation="MVKIIMHGCNGHMGQVITGLVKEDANAEIIAGIDMNDNGQNDYP
                     VFKSLAECDVPADVVVDFSSYKAADALLDACKEKKLPLVLCTTGLTEDQLKKVQETSK
                     EIPVLRSANMSLGVNTLMKLVQAAAKVLAEAGFDMEIVEKHHKLKCDAPSGTAIALAD
                     SLNAAMDNQYHYVYDRSQRHEQRDPKEIGISAVRGGTIVGDHDVIFAGPDEVVTLSHR
                     AYS"
BASE COUNT         2232 a         1916 c         2085 g         1855 t
ORIGIN      
        1 ggctgccgcc agcactgcga ctgcacacac aatcgcgatt acttttttct tcatacgctt
       61 cctcccactt ttctattttt gcaaagaatt tttcagtttt cccaagttag ctctatcgta
      121 tcacattttg ttttttgttt tcttactttt tccttactct tttttaagta aatactggat
      181 tttttctgaa aatagaattt tgacttttcg catagctctg aagagcccca tctcgattcc
      241 gctgcgctgc atctcaatgt tgcttctcgc catatgaaat tccaagaaaa actgctcatt
      301 catgcaagca tgattcgcaa ttttttcttc catttcgcat ggctctgaat aattaccaaa
      361 taaagaggaa aagatgagga aaactatgct ccaagagtgg aaaagaaaag gaaaaaaatt
      421 ccgccatgag gaaaaagatg gagaagaaac gacggagctt tctgtgaaaa tacgggcggc
      481 aaaatttcgt gatctgccga tgagtctgcg gattctgatc gtgaatctca gtctgtttct
      541 gattctgtgc gccgtatttt ttatggcagg cgcgtatctg tatgcgccgc aggcggcagg
      601 acggaattac gtggaagcat gcctggcagg agactggaac agtgcatacg atgtatgcca
      661 gttcccggac agcacgtttc tgtcgcggaa gaactacgtc aatgcaatga cctggaaagc
      721 aaaacaggac ggcactgacg ggcaggaaac accggagatc aagagctttt tcatgcgcag
      781 aaaacagaat cctgagaccg gggaaaacag gatttatact gtgcggtaca cgttaaaagg
      841 catgagcgac tcgcaggaag aaacattgga gatcgccgcc ggggataccg taaaatggaa
      901 cttcaaagag tggtacgttg tgccgaaaga cagctgcgtg acggatgtcg aaatcaccgt
      961 cccggcggat gcctcgttct atctggacgg cgtgcaggtc gggaaaaagt atttaaaaga
     1021 gacggcagat aacgtcagca tctacaaaat cccgtatctc ttcctcggcg gccacaccat
     1081 tgaactgacc gaaaaacaga aagacccata ccgcgagatc atcctcgtgc aggacaacag
     1141 cagcatggaa ttcctgccgg atttaaaact caacgattcc accggcaaaa ccatcgccga
     1201 ccgcgcagaa gagtccctgg acaaggtatt cacagccgca gccgccggaa aatccttcag
     1261 caccatcaaa aatgaatttt ccgccgacac cgccgtacag aaaaccgcca aagaacagta
     1321 ccagcagctc tgcgatgcct acctgaacag cgacaccaac accggaatca ccagcatcac
     1381 cgtgagcagc gtctccacaa ccgtaaccaa cgaatccaac cagatgaaga tcgaaaccaa
     1441 cgtgaccgct gcgatcgagc gacgaacccg tttcctccat ttcttccgca aaaccaaaat
     1501 agagaccgtc acctggaaac tcgagtccgt tgtgacgctg gaaaacgaaa aatgggtgat
     1561 gggcagcgat ttcctgaagg gcgttgagct gtcgaagtga ttttgagaaa acggttgata
     1621 tttctgagaa ctctgcgtac aatagaaata ccaagagaga aatctcttcc taaaatagga
     1681 catgaggcat aaggtgtttg cttccgttgt tctggcaacg ttaaaacttt gcagttactc
     1741 ttaagcagag aggataatca ggctggacag gtgaaagacc tgtgatttgg tggcaaagca
     1801 ccagaaagaa accaggtttt ttaaaagaga gtgacaaaga gtgattttgt atccgctttt
     1861 accatgggtg cagagtcact ctttttttgt gacaagatgt taagaaacga taaacattaa
     1921 tttaccagaa gtggaaaaat gtaagaattt gtgccttgca tcttaggtca gatcacgtta
     1981 tgatagtaag tagaaaaatg tcgaaaaacg tttgtgttac gatggaagaa agttccgcaa
     2041 aggggcggga cgatggagta taaacagatc atatggaaag aaaatcattg aaacagagaa
     2101 gaaagcagat gcgccgcaga cagatgatgc agaaagtatc acggatttcc ctgtgtgtgg
     2161 taatcggcct tctttttctt gcgcttatct ggaaatttgc gggagcgcag cttgcgaaaa
     2221 aaggaattgt actgagtaat gacacagccg caaagacggt gagcgagacg gcgccggagg
     2281 cggaggacgg atcgggaaat gcgggaatta agaagcttgc ggagaccacg ccgccgaatg
     2341 agacgccggg atggcagtac agctcgtcgg gctggtggta cgcaacggat gccacaacgt
     2401 actacgtcaa cggctgggca gatatcgacg gaaaccagta ccattttgat tccaatggtt
     2461 acatggcgac cggctggaaa ccgatcggcg gaaaaggctg ttactttgat gaccagggtg
     2521 tttaccagcc agaccgcaac ggctcgatga tggtagccct gacctttgat gacggcccag
     2581 gcgtctatac cagcacgctt ctcgatccgc tggagcagta cggggcacag gcgacgttct
     2641 ttatgctcgg cagcaatgtg gaaaaatacg gtgcagatgt gatcccgcgc atggcggcga
     2701 tcggctgtga gcttggaaac cattcctatg ctcatccgaa tatgaaagaa ctgagcctcg
     2761 acgacggcct cgcccagttc cagatgactg atgatctgat tgcccagtac aacaacggcc
     2821 agggcgcatc cgttgtccgt ttcccgtatg gaaacagcac cgatgaactg ctcgcatcta
     2881 tcggccgccc gagcatcatg tgggatgtcg ataccctgga ctggcagaca aaaaatgtac
     2941 aggcaaacat cagcgccgtt ctcgacagtg taagcccggg agacattatc ctgatgcatg
     3001 atatccacga gacgaccgta gaatcgtgtg caacgatcgt gccggagctg atcaaccgcg
     3061 ggtatgagct tgtcacgatt catactcttg cagcggcaaa cggcgtggat cttgcagcgg
     3121 gagagacgta ttacggcttc catggcggaa cgacaaacga atagaaaaca gaatagaaaa
     3181 acgacaaaga accgccggtt tactctcaaa tcaagagtga accggcggtt cttatgtcat
     3241 tttatgaaaa tttatttcat tgccgctctc ttacgaagtc tggaatccag ctctttctta
     3301 cggatacgta aggaaaccgg agtaacctca agaagctcgt ccttgtcgat gaagtccaga
     3361 gcctgctcca gggaaaggat tctcggcgga gtcagacgca gtgcctcgtc ggaggaagaa
     3421 gtacgggtgt tggtcaggtg tttcgtcttg cagacgttca gctcgatatc ctcggcctta
     3481 ccgttctgtc cgataaccat accggcgtaa accttctcgc caggtccgat gaacagagta
     3541 ccgcgctcct gtgcggagta cagaccgtag gtaacggact cgcctgcctc gaaagcgatc
     3601 agggaaccct gtttgcggta ttcgatgtcg cccttgtacg gtgcgtaatc ttcaaactcg
     3661 gtgttcagga taccagttcc tttggtggag gtcatgaaga ttccacggaa accaatcagg
     3721 cctcgtgccg ggatttcgaa ctccagacgg gtggaaccat cgcttgcgcg gctcatgccg
     3781 tgaagctcgc ctttacgctc gctgaggagc tggatgacag taccggagaa ctcatccgga
     3841 acgtcaacgt atgcgatttc cattggctcg agtttgcgtc cgcgctcgtc ctctttgtag
     3901 ataacctctg ccttacttac tgcgaactca tatccctcac ggcgcatgtt ttcgatcagg
     3961 acggacagat gcagctcacc acggccggaa accttgaagc agtctgcgga atcagtctcc
     4021 tcgacgcgga gagaaacgtc agtgttcagc tcacgcatca gacggtcacg caggtggcgg
     4081 gaagttacgt atttaccttc tttaccggcc agcgggctgt cgtttacgat gaagttcatg
     4141 gaaatagtcg gctcggagat tttctggaac gggatggaaa ccgggttgtc cagatcgccg
     4201 cagagagtat ctccgatgtg gatatccggg ataccggaaa cagcaacgat agaaccgatg
     4261 cctgcctcag taacctctac ttttttcagt ccgtcaaatt catacagttt gctgaccttt
     4321 acttttttct tcttggtcag atcgtggtgg ttgaccagca taacgtcctg gttcacgcgg
     4381 atcgttccgt tttcaacttt gccgacaccg atacgtccaa cgtattcgtt gtagtcgatg
     4441 gtactgatca gaagctgtgt gccagcttcc ggatcgccgg tcggagccgg gatgtatttc
     4501 aggatggtct cgaacagagg agtcatatcc ttcggggtat cgttcagatc gagtaccgca
     4561 tgacccatct ttgcggatgc gtacagaaac ggacagtcaa gctgttcgtc agatgcatcg
     4621 aggtccatca gaagctcgag cacttcgtcg ataacttcat ccgggcgtgc ttccgggcgg
     4681 tcgatcttgt tgatgcagac gataacggac agatccagct cgagtgcttt tttcagcacg
     4741 aatttggtct gcggcatagc gccctcgaat gcgtctacaa ccagaataac gccgtctacc
     4801 attttcagaa ctcgctcaac ctcgccgtgg actcgaacga tatcgagcgt gagcgtggaa
     4861 tcaccattcg ttcctttgta gtaaactgct gtatttttgg aaagaatggt gattccacgc
     4921 tcacgctcga tatcgttcga gtccatgaca cgttcctgaa cctcctgatt ggcacggaaa
     4981 acgccgctct gtttcagaag ctcgtctacc agtgtagttt taccatgatc gacatgggcg
     5041 atgatggcaa cgtttcttac atcttctctc gttgttttca tttcttcatt cctcttccta
     5101 tgggttgacc ctgccgggtt caatttttca aaatttcaag ccggccggga atgtttatca
     5161 cagcacaaag gcaggtgcct tgccgttttg tctctgctgg atgcctgcct atctgaacta
     5221 ttatagcaca gaacagagaa aaaaaacagg gaagatgtga cattttttgt attgcaatgg
     5281 tagaaaatac gaagcatgtg gtgcggtttt tacagaaaca tatacaaaaa attgggcaga
     5341 atgctgattt gaatagaaat aaaaaaatct ggaaaaaagt ggttctgaaa aagaaatgtg
     5401 gttcatacat taggattata gatcagcaaa gtaccgatca gaaatcaata ttcagaaaga
     5461 aaagacaaag gaaggggatt ttcattatgg cagacaagat gattgaaaac aatcaggtgg
     5521 ttatcatggg agagattgtc tccccgttta cattcagcca tcaggtattc ggagagggct
     5581 tttacctcgt cgatgtgatg gtcaagcggt tgagtgaatc gcaggacatc attccggtga
     5641 tggtctccga gcggctggtg gacgtatcac aggattacca gggtgagtgg atctgcatca
     5701 gcggtcagtt ccggtcctac aaccgccacg aggagaagaa gaaccgtctg gtactctccg
     5761 tatttgcgcg ggaattaagc ttcgtggagg aagaggacga ttccgtcaaa tccaaccaga
     5821 tttacctcga tggatacata tgtaaaccgc cggtctaccg gaaaacacct ctgggaagag
     5881 agatcgccga ccttctgatc gcggtcaacc gtccgtatgg aaaatcagat tatatcccgt
     5941 gcatctgctg gggacggaat gcgcggtatg cctctgcgtt tgccgtgggc ggccacgttc
     6001 tcatctgggg acggatccag agccgggaat atatgaagcg gctgagcgag acggaaatgg
     6061 aaaagcggac cgcatatgag gtttcggtga gcaagctgga atatagtgag agttaaagcg
     6121 cgatccgctg ttgacaagaa aatttaatga tgctatagta aaagcagaac tgaagtggat
     6181 agaggtcgca ttacttcaag agtacctttt cggatgcctc agcagcaaaa gaagaagagg
     6241 aaaagggaag aatgccgaag gacatccggc gctgaaccgg tatgctcctg ggcgcaggtt
     6301 gaaaaaatct gcgactgtca tcgagagatg gagagctatc tgatcataga tactgcgtat
     6361 gtgtgaattt ggaggcgatc ttatcgggga tgaggcgcct cttttttaaa cggaaagtga
     6421 aattttccgt gggagataag ctccggcgac tgatttctta tggttttatc tgacacaaca
     6481 tacaggagga aaagagaatg tctattttta aaggtgcagc tgttgcaatt atcacaccga
     6541 tgaccgaaac acaggaagtg aattacgaaa aactcggaga actgattgag gagcagatcg
     6601 ccggcggaac cgacgccatc gttatctgcg gaaccaccgg tgaggcttcg accctgtccc
     6661 atgaggagca tctggatgcc atcaaatata ccgtggaaaa agtaaatcac agaatcccgg
     6721 taattgcagg aaccggctcc aacagcacgg agacagcaat ttatctgtct caggaagcag
     6781 aaaaatacgg cgtggacgga cttcttctcg tttctccgta ttacaataaa gcaacacaga
     6841 agggcctgat tgcacacttt accgcaatcg cagacagcgt caaagtgccg gtcatccttt
     6901 acaacattca gggcagaacc ggcgtaaaca ttgcaccgga aacgatcgtc tatctgttca
     6961 acaacgttga gaatatcgtt gcagtcaaag aggcttccgg aaacatttcc caggtcgcaa
     7021 aaattatgca gctgaccgat ggaaagatcg atctgtactc cggaaacgac gatcaggttg
     7081 tgccgatcct ttcccttggc ggaagcggtg taatctccgt tctttccaac gtagcaccgc
     7141 gtgagaccca cgacatggta cagaaattcc tcgacggcga tgtggccgga tccagagaaa
     7201 tccagctgag agcactgccg ctcatcgatg cgctgttctg cgaagtaaac ccgattccgg
     7261 taaaagcagc gctgaacctg atgggcaaag aggttggacc gcttcgtctg ccgctgaccg
     7321 agatggagcc gcagaaccag gagcgcctgg caaaggctat gaaagatttt ggaatcaaac
     7381 tggcttaagt taagaataca cgaagtaaag aaggaaaaag acaatggtta aaattattat
     7441 gcatggctgc aacggccata tgggacaggt catcaccggc ctggtaaaag aagatgcaaa
     7501 tgcagaaatc atagccggaa tcgatatgaa cgacaacggc cagaacgatt acccggtatt
     7561 caaaagcctg gcagaatgcg atgttccggc agacgtagtc gttgactttt cttcctacaa
     7621 ggcagccgac gcactgctcg atgcctgcaa ggagaaaaaa cttccactcg tcctctgcac
     7681 cacaggcctc accgaggatc agctcaaaaa agtgcaggag accagcaaag aaatcccggt
     7741 tctccgctct gcaaatatgt cccttggtgt gaatacgctg atgaaactgg tacaggcagc
     7801 tgcaaaagtg cttgccgaag caggctttga tatggagatc gtggagaaac accacaaact
     7861 gaaatgcgac gctccaagcg gaaccgccat cgccctggca gacagcctga acgcggcaat
     7921 ggacaaccag taccactacg tatacgacag aagccagcgc cacgagcagc gtgacccgaa
     7981 agaaatcgga atctctgctg tacgcggcgg caccatcgtc ggcgaccacg acgtcatctt
     8041 cgcaggcccg gacgaggttg ttaccctgtc ccacagagca tattccaa
//
LOCUS       DAOMUF010000095         8084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2315_length_8084_cov_2.737825, whole genome shotgun sequence.
ACCESSION   DAOMUF010000095 DAOMUF010000000
VERSION     DAOMUF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_8084_cov_2.737825"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLN77_06610"
     CDS             complement(<1..598)
                     /locus_tag="PLN77_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRM43983.1"
                     /translation="MDILLWHEILEPYELAVKELQVKFRHLIKEHHGKGLYSPIESVS
                     GRVKSVSSILEKMQRKGIVPEEMEEQVEDIAGIRIICQFVEDIEKVADLIQKRSDIEI
                     KSEKDYIRHMKDSGYRSYHLIVYYTVETMNGPKRIQVEIQIRTMAMDFWATIEHSLQY
                     KYKANIPDHIRERLSAAAKAIIVLDNEMSSVRSEIMDAQ"
     gene            complement(757..2217)
                     /locus_tag="PLN77_06615"
     CDS             complement(757..2217)
                     /locus_tag="PLN77_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRM43984.1"
                     /translation="MSVLAGLKPERVFYYFEELCRIPHGSGNTKEVSDYLVQFARDHE
                     LSWYQDASNNVIIRRPASKGCELAPTVILQGHCDMVCEKKPGSSHDFTKDGLELHIDG
                     DEIYARDTTLGGDDGIAVAYMLAVLESDDLQLPAIEALITTDEEIGLLGAAALNGNEL
                     KGRTLLNMDSEEEGILTVSCAGGMTAMMDLPVRRSEVMGEQMEVTISGLAGGHSGTEI
                     HKNRANSNILAGRFLYELAGKMDYALIELEGGLKDNAIPRTTRMLLAIDGGEKEILKE
                     EASRFTENLRREYSGTDDGITVTVEKTGEGAAPALHPVSLQKTVFFLMNLPNGVQKMS
                     GQIPGLVETSLNLGILKLEPDHLHACASVRSSVGSAKEALSDKLEYLIGFLGGEFHVE
                     GAYPAWEYKEDSALRDLMVAEYEAMYGQKPAVEAIHAGLECGLFYEKLSGLDCVSFGP
                     NMKAIHTTEERLSISSVERTYQYLCRVLEQLSKRQA"
     gene            2573..3244
                     /locus_tag="PLN77_06620"
     CDS             2573..3244
                     /locus_tag="PLN77_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HRM43985.1"
                     /translation="MGARITAQLYEAEEIDRNEACYLLTFCSSPSPAFLSSYVILTAF
                     SGQIHPGFVFGVLYGSNFLTGFLFRLVFRRFRLHRHTAHRTHSVEKKEMSKQTSKGNL
                     LDTSIMNGFEIITQLGGYIILFSILSGLLMDLHPFGSASPLLCGVLEITTGIAQLAAS
                     SLPYAGKVSAVLTCAAFGGLSVAAQTNCMIHNSGLPLWPYLAGKLCCAAFAGTLGLFL
                     FAFLF"
     gene            complement(3218..3841)
                     /locus_tag="PLN77_06625"
     CDS             complement(3218..3841)
                     /locus_tag="PLN77_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRM43986.1"
                     /translation="MSSRIEQIIEEIEEYVESCKYQPLSTTKIVVNKEELEELLRELR
                     LKTPDEIKRYQKIISNKDAILADAQNKADAIIAEAQAKAREMVEQSEVMQSAYAQANE
                     TVNNANQQAQEILDAATNDANSIRLGAIGYTDDMLANLSNIMTTALNDAGEKYNSFVE
                     SLTSCYNTVVQNRSELSPQGAPEEEKPEEEVVVPEYDPASEEESEEE"
     gene            complement(3853..4365)
                     /gene="coaD"
                     /locus_tag="PLN77_06630"
     CDS             complement(3853..4365)
                     /gene="coaD"
                     /locus_tag="PLN77_06630"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRM43987.1"
                     /translation="MKTAVYPGTFDPVTYGHLDVILRASKLFDRVIVGVLHNSSKSPL
                     FSVEERVNILKKATQDIPNVEVRSFSGLAVDFAKECQAHTIVRGLRAITDFEYELQMA
                     QTNRVLEPEVDTTFLITSLEYAYLSSTVVKEVAAFGGDIHKFVPDFVEKEIRAKYAAR
                     NSEGMPQDKR"
     gene            complement(4362..4940)
                     /gene="rsmD"
                     /locus_tag="PLN77_06635"
     CDS             complement(4362..4940)
                     /gene="rsmD"
                     /locus_tag="PLN77_06635"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRM43988.1"
                     /translation="MRVIAGKCRSLPLKTVPGRQTRPTTDRIKETLFNMLQPYLPDAR
                     FLDLFAGSGAIGIEALSRGAERAWFVEQNKAAADCIRQNLKFTKLEQQAVLLPMEAHA
                     ALQSLQGQEPFDCIFMDPPYDKGLEKDVLYAFRDASFIGEDTLIIVEASLNTDFDWAE
                     DAGYEILKKKIYKTNVHVFLTPKSKHEGVKES"
     gene            complement(4981..5382)
                     /locus_tag="PLN77_06640"
     CDS             complement(4981..5382)
                     /locus_tag="PLN77_06640"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRM43989.1"
                     /translation="MNIGIIAHNSKKSLIEDFCIAYKGILMKHEVYATGTTGRRIEEV
                     TNLHVHKFLPGSVGGDKQFTEMIERGDMDMVIFFYNPTMINPKEPDVYKIIRTCDQYN
                     IPIATNIGTAESLILGLARGDLDWREQLKAD"
     gene            5678..5899
                     /locus_tag="PLN77_06645"
     CDS             5678..5899
                     /locus_tag="PLN77_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HRM43990.1"
                     /translation="MANRSSNTAAVPEAKGALDKFKFEVASELGVPLSDGYNGNLTSK
                     QNGSVGGYMVKKMIEQQERQMSGGSSGQY"
     gene            complement(6012..6530)
                     /locus_tag="PLN77_06650"
     CDS             complement(6012..6530)
                     /locus_tag="PLN77_06650"
                     /inference="COORDINATES: protein motif:HMM:NF040112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HRM43991.1"
                     /translation="MVKKKPAAQAKTAENETVKVETKAAEKTTAAEKEAVKEVAKEVV
                     KETAAAEEAPAKAEEKPAAKAAKTTRTTAKKTTTKKAAAPKKPAAAKKPAEKKAPAKA
                     AKKEAEQEMILQFGGREIKEKDLYERIQQIWIEGYGKKAEELKSLKVYVKPEEFTAYY
                     VINDDVTGSIDL"
     gene            complement(6794..>8084)
                     /locus_tag="PLN77_06655"
     CDS             complement(6794..>8084)
                     /locus_tag="PLN77_06655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRM43992.1"
                     /translation="LTNAQMRVWGELERDLKGHTRMARLVQGDVGSGKTILAFLAMIM
                     TAENGYQSALMVPTEVLARQHYESLCTLLKENNLTEYNPRLLTGSTKAKERREIYASL
                     EDGSCKMVIGTHALIQEKVQYKNLALVITDEQHRFGVRQRTALAEKGEPPNVLVMSAT
                     PIPRTLAIILYGDLSISVIDELPAKRLPIKNCVVDTSYRPKAYRFITRQVELGRQAYV
                     ICPMVEESEGLEAENVTDYTQLLKKELPGITVEMLHGQMKAAQKNEIMERFASGEIQV
                     LVSTTVVEVGVNVPNATVMMVENAERFGLAQLHQLRGRVGRGEHQSYCIFIRGNKDQE
                     TAKRLEILNHSNDGFYIASEDLKLRGSGDLLGTRQSGDMEFQMADIFRDADILQKASE
                     AASELLADDPYFEKPEHELLKKVMKSYLDLENHDIGL"
BASE COUNT         1654 a         2199 c         1743 g         2488 t
ORIGIN      
        1 tctgtgcatc cataatctca ctccgcacag aggacatctc attatccagc acaataatcg
       61 cctttgcagc tgcacttaag cgctcccgga tgtgatccgg aatgtttgcc ttatatttgt
      121 actgcagcga gtgctcaatc gtcgcccaga aatccatcgc catcgtgcgg atctggattt
      181 ccacctggat gcgcttcggg ccgttcatcg tctccacggt atagtaaaca atgaggtgat
      241 agctccggta accgctgtcc ttcatgtggc ggatgtagtc tttctcgctc ttaatctcaa
      301 tatccgaccg cttctggatc aggtcggcaa ccttttcgat atcttcaacg aactggcaga
      361 tgatgcggat gcccgcgata tcctccacct gctcctccat ctcttcgggc acaatgccct
      421 ttctctgcat tttttccaga atactggaaa cactcttcac acggccgctc acgctctcga
      481 tcggtgagta cagccctttt ccgtggtgct ccttgatcag atgccggaat ttcacctgta
      541 attccttgac agccagctcg tatggttcca gaatctcatg ccataacagt atatccatgt
      601 cccctaatct ccttttttgc tcctgctttc agccattttt cttatcggat ggtaaacgct
      661 ttggaaattc aatccccacc ctgttacttt atgcctgtct tttctgtttt atgactgttt
      721 ttcagaaact tttttgaaat agacacgggc agttttttat gcctgccgtt tcgaaagctg
      781 ctccagaacg cggcacaggt actgatacgt tctctccacg gaggaaattg aaagccgctc
      841 ctcggtcgtg tggattgctt tcatgttcgg gccgaaagat acacagtcaa gaccggaaag
      901 cttttcatag aacagaccgc attccagtcc ggcatgaatc gcttccactg ccggtttctg
      961 tccatacatg gcttcatatt ccgccaccat cagatcgcgg agggcagaat cctctttata
     1021 ctcccatgcc gggtacgcgc cctctacgtg gaactcaccg cccaggaaac cgatcagata
     1081 ctccagcttg tcggaaaggg cttcttttgc acttcccacg gagctccgca cactcgcaca
     1141 cgcatgcagg tgatccggct ctaatttcag aatgccgagg ttcagggatg tttccaccag
     1201 tcccgggatc tggccgctca tcttctgtac cccgttcgga agattcatca ggaaaaagac
     1261 ggttttctgc aggctgaccg gatgaagcgc cggtgcagcg ccctcgcctg ttttctctac
     1321 cgtaacggta ataccgtcat ctgttccgct gtactctctg cgaagatttt cggtgaagcg
     1381 tgatgcttcc tcttttaaga tctctttctc tccgccgtcg atggcaagaa gcatgcgggt
     1441 tgtccgcgga atggcgttgt cttttaaacc gccctccagc tcgatcaggg cataatccat
     1501 ctttcctgcc agttcgtaca ggaaacggcc cgccagaatg ttggagttgg cacggttttt
     1561 gtggatttcc gtaccggaat ggccaccggc aagaccggaa atcgtcactt ccatctgctc
     1621 acccatcact tcgcttctgc gcaccggcag atccatcatg gctgtcatac cgcctgcgca
     1681 cgatacggtg agaatgcctt cctcttcaga atccatattt aacagtgttc ttccctttaa
     1741 ttcattcccg tttaatgctg ctgcgccgag cagtccgatc tcttcatccg tcgtaatcag
     1801 tgcctcgatg gccggaagct gcagatcatc cgattccagc acagccagca tataggcaac
     1861 ggcaattccg tcgtctccgc caagcgtcgt gtccctggca taaatctcat cgccgtcgat
     1921 gtgaagctca aggccgtcct tcgtaaaatc gtggctgctg cccggcttct tctcgcagac
     1981 catatcgcag tggccctgca gaatcacggt cggagccaat tcacatccct tcgatgccgg
     2041 gcggcggatg atcacgttgt tggacgcatc ctgataccag gataattcat ggtcccttgc
     2101 aaactgaacc agatagtcgc tcacttcttt tgtgtttccg gagccgtgcg gaatgcggca
     2161 gagctcttca aaataataaa atacccgttc cggttttaat cctgctaata cgctcatttt
     2221 ttcttctccc atcttctttt tttcaaagct tcgtcatact ctattttaga aagcgggagg
     2281 aggttcgaac gtcatgaaaa aatcactttt atttctctct tccctgcttc tttttttctg
     2341 tcttcttatc tttcccgcgc ggactttttt ctatgcctgc gagggtgtca atctctggtt
     2401 tcataccatt cttccgtcac ttctgccgtt tctgattctg acatctcttc ttctggaaac
     2461 aggagccgcg cggcggatct ctgcccgtgc agaagttttt tttcaaacgg ttttcggtct
     2521 ttccccggct gggttttatg cactttttct cggtcttttc tgcggttttc ccatgggagc
     2581 gcgcatcact gcccagctct atgaggcaga agaaattgac cggaacgaag cgtgctatct
     2641 tttaaccttc tgcagtagtc cgagtcctgc ttttctgtct tcctatgtta tcttaactgc
     2701 attttccgga cagatccatc ccgggttcgt cttcggcgtg ctctacggtt ccaattttct
     2761 aactggtttt ctgttccggc ttgttttccg ccgcttccgc ctgcaccggc acaccgccca
     2821 tcggacacat tccgtggaaa aaaaagagat gtcaaaacag acttccaaag gaaacctgct
     2881 ggacacctct atcatgaacg gatttgaaat cattacacag cttggcggat acatcatcct
     2941 gttttccatt ctgtccggac ttctgatgga tctgcatccg tttggttccg cctcgcccct
     3001 gctctgcggc gtgctggaaa tcacgacggg aatcgcacag cttgccgcgt cctctcttcc
     3061 ttacgccgga aaagtctctg ctgtccttac ctgtgccgcc ttcggcggtc tctctgtcgc
     3121 tgcccagaca aactgtatga tccataacag cggtctgccc ctctggcctt atcttgccgg
     3181 gaagctctgc tgtgctgctt tcgcaggaac gcttggttta ttcctcttcg ctttcctctt
     3241 ctgatgccgg atcatattcc ggtacgacaa cttcttcctc tggcttttct tcttccggag
     3301 caccctgcgg ggagagctca cttctgttct gaacaacggt attgtagcag cttgtcaggg
     3361 attctacaaa gctgttgtat ttctctcctg catcgtttaa tgcagtcgtc ataatattgc
     3421 ttaagttggc aagcatatca tcggtatatc cgatggcacc gagacggatg ctgttggcat
     3481 cgttggttgc agcatccagg atttcctgtg cctgctggtt tgcattgttg acggtctcat
     3541 ttgcctgtgc gtatgctgac tgcatcactt cactctgctc taccatctct cttgcctttg
     3601 cctgtgcctc tgcaatgatg gcatctgcct tgttctgtgc atctgccaga atcgcatctt
     3661 tgttgctgat gatcttctgg taccgtttga tctcgtccgg tgttttcaga cggagctcac
     3721 ggagaagctc ttctaattct tctttattta caacgatttt ggtggtagac agcggctgat
     3781 atttacagct ctctacatac tcctcaattt cttcgatgat ctgttcaatt ctgctgctca
     3841 ttttaattct ccttatcttt tatcctgcgg cattccttct gaatttcggg cggcatactt
     3901 tgcgcggatt tctttttcca caaaatccgg aacaaatttg tgaatgtcgc cgccaaaagc
     3961 agctacttct tttactaccg tggagctgag gtacgcatac tccagcgagg taatcagaaa
     4021 cgtggtatct acttccggtt ccagaacgcg gttcgtctgt gccatctgca gttcatattc
     4081 aaagtctgtg atcgcgcgga gtccgcggac gatcgtatgc gcctggcatt ccttcgcaaa
     4141 atcaacagcg agcccgctga aagaacggac ttcgacattt ggaatgtcct gggtcgcttt
     4201 ctttagtata ttaacacgtt cttccacaga aaacaacgga gattttgagg aattatgtaa
     4261 aactcccacg attacgcggt caaaaagctt ggatgcacgc agaatgacat ccagatgacc
     4321 atacgttaca gggtcaaaag ttcccgggta tacggctgtc ttcatgattc ttttactcct
     4381 tcgtgtttgc ttttcggggt aaggaataca tggacatttg tcttatagat ttttttcttc
     4441 agaatttcgt atccggcatc ctccgcccag tcgaaatcag tattcagcga tgcttctaca
     4501 ataattaagg tatcctcacc gataaaagag gcgtcgcgga acgcatacag cacgtccttt
     4561 tccagtccct tatcgtatgg cgggtccata aaaatgcagt caaaaggctc ctgtccctga
     4621 agcgactgca gagctgcatg cgcttccatg ggcaggagca ctgcctgctg ttccagcttc
     4681 gtaaatttta aattctgccg gatacagtct gctgcagctt tgttctgctc tacgaaccac
     4741 gcgcgctcgg cgccgcggct caacgcctcg attccgatcg ccccgcttcc ggcgaacaga
     4801 tcgagaaaac gcgcatccgg cagatacggc tgaagcatat taaataaggt ttctttgatc
     4861 cggtccgtcg tcggccgggt ctgtctgccg ggaaccgtct ttaatggaag actccggcat
     4921 tttcctgcta ttactctcat gttggtctta ttttcgtcct tactgttttt gtttgatgga
     4981 tcagtcggct ttcaactgct ctctccagtc cagatctcct cttgcaagtc ccaaaatcag
     5041 agactccgcg gttccgatat ttgttgcaat cgggatattg tactggtcgc aggtacggat
     5101 aattttgtag acatccggct ctttcggatt gatcatcgtc ggattgtaaa agaaaattac
     5161 catatccata tcgccgcgtt caatcatctc ggtaaactgt ttgtctccgc ctacgcttcc
     5221 aggcagaaat ttgtgtacat gaaggttcgt cacctcttca atccgccgtc cggtggttcc
     5281 ggtggcgtac acctcatgtt tcatgaggat tcccttgtat gcaatacaga aatcctcgat
     5341 cagactcttc ttgctgttgt gtgctatgat tccaatattc attactgcaa accgcccctt
     5401 ctcctgtttt tactaccatg tacagcaatt ataacaaatt tattttcaat acgcaattct
     5461 ttttgtgaat tttttacaca gtttttatat tttttctgcc gttccatttt tccttcttct
     5521 tttactttct ccgcttttcc ggaaaaaata cagaatctgg aagtttattt ttgttttctt
     5581 tccacatact atgaccgtag cagaacagat gaaaaacaca tccggatctg cttcacagca
     5641 gcttttaaca agcacaaaga taaaggaggg aatgaacatg gcgaatcgtt catcgaatac
     5701 cgcagcagta ccagaagcaa aaggcgctct ggacaaattc aaatttgagg tggcaagcga
     5761 actcggcgtg ccgctcagtg atggctacaa cggtaacctg acttccaaac agaatggttc
     5821 tgtcggaggt tatatggtca aaaagatgat tgagcagcag gaacgccaga tgtccggcgg
     5881 ttcttctggc cagtactaat catcccggta taacatcctg atgccggaac aggcatcaaa
     5941 aagcgtgagc tcttttcttt tcggaaaagc tcacgctttt ttcttgctcc tgcagagctg
     6001 gtacataata cttacagatc aatgcttccg gttacatcat cattgattac gtagtaagca
     6061 gtaaattcct ccggttttac ataaaccttc aggctcttta attcctctgc tttcttgcca
     6121 tatccttcga tccagatctg ctggattctc tcgtacagat ctttctcctt gatttctctt
     6181 ccgccgaact gcagaatcat ctcctgctcc gcttctttct ttgctgcttt tgcaggtgct
     6241 tttttctctg ccggcttttt cgctgctgct ggttttttcg gtgcagccgc tttctttgtc
     6301 gttgttttct ttgctgttgt gcgggttgtt tttgcagctt ttgctgccgg tttttcttct
     6361 gcttttgcag gtgcttcctc tgctgctgca gtttccttta caacttcttt tgcaacttct
     6421 tttacagctt ctttctctgc tgcggttgtt ttttctgctg ctttggtctc tacttttact
     6481 gtctcgttct ctgctgtctt agcctgggca gctggttttt tctttaccat atctgtttcc
     6541 tcccatttag accggccctg tatattttgg aagagccgtc ctgcgaacta tcattcaggc
     6601 gaacgcttga cactcacggt ccgcacattt tttgattcct tgtttatgat acatgaattt
     6661 ttttggcatt tcaagtaata tctgatttcg cacgaatttt ccacgaaatt gtacagtcat
     6721 tttccatact atccaatcag gctgttattt ttatccgatt ttgcatttta aagccggatt
     6781 tttttgtaat ttttcacaat ccaatgtcgt ggttttccag atcaagatac gatttcatta
     6841 cctttttcag cagttcatgc tccggttttt caaaataagg atcgtcggca agcagttctg
     6901 aagccgcctc actcgctttc tgcaggatat cggcatcccg gaaaatatct gccatctgga
     6961 attccatatc cccgctctgg cgcgttccca gcagatcgcc gctgccgcgc agtttcaaat
     7021 cttccgaggc aatataaaac ccgtcgttcg aatggttcag gatttccaga cgtttggcgg
     7081 tctcctgatc tttgtttccg cggatgaaaa tacagtagga ctgatgctct cctcttccga
     7141 cacgtccgcg gagctgatgg agctgtgcaa ggccgaaccg ttcggcattt tccaccatca
     7201 tcaccgtcgc attcggcacg ttgacaccga cctccacgac tgtcgtcgag acgagtacct
     7261 ggatttctcc gctggcgaac cgctccatga tctcgttttt ctgcgccgcc ttcatctgtc
     7321 cgtgcagcat ctctactgtg atgcccggca gctctttttt caggagctgt gtatagtcgg
     7381 tgacgttttc cgcttccaga ccctcgctct cctccaccat cgggcagatc acatacgcct
     7441 gacggcccag ttcgacctga cgcgtgataa agcggtaagc ttttggacgg taagacgtat
     7501 ccaccacaca gtttttgatc ggcagacgct tcgccggaag ttcatcaatc acagaaatcg
     7561 aaagatctcc gtacagaatg atagccaggg ttcttggaat cggcgttgca ctcataacga
     7621 gaacattcgg cggttcccct ttttcagcaa gtgccgttct ctgccggaca ccgaagcggt
     7681 gctgttcgtc tgtgatgaca agcgccagat ttttgtactg taccttttcc tgaatcagtg
     7741 catgtgtgcc gatcaccatt ttgcagcttc cgtcttccag actggcatag atttcccggc
     7801 gttcttttgc tttcgtggat ccagtcagaa ggcggggatt gtattccgtc agattgtttt
     7861 ctttcaacag agtgcagagc gattcataat gctgccgcgc cagaacctcc gtcggaacca
     7921 tcagcgccga ttggtagccg ttctcagctg tcataatcat tgcaaggaat gccagaatcg
     7981 tctttccgct tccgacatct ccctgtacca gtcgcgccat gcgggtatga ccttttaagt
     8041 cccgctccag ttcgccccac acgcgcatct gtgcattcgt gaga
//
LOCUS       DAOMUF010000096         7921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2383_length_7921_cov_3.310959, whole genome shotgun sequence.
ACCESSION   DAOMUF010000096 DAOMUF010000000
VERSION     DAOMUF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2383_length_7921_cov_3.310959"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /gene="rpsU"
                     /locus_tag="PLN77_06660"
     CDS             complement(<1..117)
                     /gene="rpsU"
                     /locus_tag="PLN77_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRM43993.1"
                     /translation="MSNVIVKENETLDSALRRFKRSCAKAGIQQEIRKREHYE"
     gene            complement(323..2962)
                     /gene="alaS"
                     /locus_tag="PLN77_06665"
     CDS             complement(323..2962)
                     /gene="alaS"
                     /locus_tag="PLN77_06665"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRM43994.1"
                     /translation="MEKYGVNELRKMFLEFFESKGHLAMKSFSLIPHNDKSLLLINSG
                     MAPLKPYFTGAEIPPRRRVTTCQKCIRTGDIENVGKTARHGTFFEMLGNFSFGDYFKH
                     EAIAWSWEFLTQVVGLDPNRLYPSIYQDDDEAFDIWNKEIGIPADRIFRFGKEDNFWE
                     HGAGPCGPCSEIYYDRGEKYGCGKPGCTVGCECDRYMEVWNNVFSQFNNDGEGHYTDL
                     IQKNIDTGMGLERLAVVVQDVDSIFDVDTIQALRNKVCEISGKEYDKDHETDVSIRLI
                     TDHIRSATFMISDGIMPTNEGRGYVLRRLIRRAARHGRLLGIDGLFLARLSASVIEGS
                     KDGYPELEEKKDFIFNVLNNEETQFNKTIDQGLHILSELEEKMQSEGKKVLDGQDAFK
                     LYDTYGFPLDLTKEILEEKGYGIDEDGFHAAMEEQRERARSSREVTNYMGADATVYDE
                     IDPSVTTEFTGYDHLEDTSKVTVLTTETEIAESLMEGQKGTIFVEKTPFYATMGGQEG
                     DCGIIKGANGVFEVEDTIKLRGGKYGHVGVMKSGMISNGEEVTLQVNEEARKNIEKNH
                     SATHLLQKALKTVLGAHVEQKGSLVTPTRLRFDFAHFQAMTPEELEKVENLVNEKIQE
                     QIPVVTDIMDTEEAKKSGAMALFGEKYGDKVRVVSMGDFSKELCGGTHVKNTAEIGLF
                     KLVSEAGVAAGVRRIEALTGPSVTAYYKAQEEKMHEAAALLKTTPADLLEKIAHLQAE
                     AKALQAENESLKSKLAKEALGDVMDKVTEVKGVKLLAASVPDVDMNGLRDLGDQLKEK
                     LGSGVVVLASAKDGKVSLLAMVTDDAMKKGAHAGKLIKEVAAVVGGGGGGRPNMAQAG
                     GKNPEKIDEAVAKAAEVLEGQIA"
     gene            complement(3041..>3166)
                     /locus_tag="PLN77_06670"
     CDS             complement(3041..>3166)
                     /locus_tag="PLN77_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRM43995.1"
                     /translation="GVSVDGEKITDPKYAVEKAAFGEDGIVLKKGKKNFKKICVK"
     assembly_gap    3167..3176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3177..4240)
                     /gene="tyrS"
                     /locus_tag="PLN77_06675"
     CDS             complement(<3177..4240)
                     /gene="tyrS"
                     /locus_tag="PLN77_06675"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRM43996.1"
                     /translation="MQIYDELIARGLLAQVTDEDEIRDLVNNGKATFYIGFDCTADSL
                     TAGHFMALTLMKRMQMAGNKPIALIGGGTTMIGDPSGRTDMRKMLTREEIDHNAECFK
                     RQMERFIEFGEGKAMMLNNADWLLNLNYVELLREVGACFSVNNMLRAECYKQRMEKGL
                     SFLEFNYMIMQSYDFYHMFQKYGCNMQFGGDDQWSNMLGGTELIRRKLGKDAYAMTIT
                     LLTDSQGKKMGKTAGNAVWLDPNKTSPFDFYQYWRNVDDADVLKCIRMLTFLPLEQID
                     EMDSWEGSKLNEAKEILAYELTSMVHGEEEAKKAQEGARAVFSTGSSEHMPTSEISAE
                     DFTDDKIDIVTLLVKAELAKT"
     gene            complement(4694..5338)
                     /locus_tag="PLN77_06680"
     CDS             complement(4694..5338)
                     /locus_tag="PLN77_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HRM43997.1"
                     /translation="MKIELEQVSPHEIEKRSFEIITEELNGRTFPEDQELVVKRCIHT
                     SADFDYADNLCFSEHAVAKGIEAIREGACIVTDTQMARSGINKKVLARHGGEALCFMS
                     DEDVAARAKETGSTRAAACMEKAAELDKKLIIAVGNAPTALVRLYELIGEGKIKPALI
                     IGVPVGFVNVVQSKELIMQTNIPYIVARGRKGGSNIAACICNALLYMMDQDRGY"
     gene            complement(5341..6811)
                     /locus_tag="PLN77_06685"
                     /pseudo
     CDS             complement(5341..6811)
                     /locus_tag="PLN77_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
     gene            complement(6823..7869)
                     /gene="cobD"
                     /locus_tag="PLN77_06690"
     CDS             complement(6823..7869)
                     /gene="cobD"
                     /locus_tag="PLN77_06690"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HRM43998.1"
                     /translation="MRQNPHGGDIYTKKCRVDFSVNVNPLGAPESVKEAVRKSAESVE
                     QYPDALCRDLTRALSEKEQLPEENILFANGAAELIFALTQALRPKKALIAAPGFAEYE
                     AALSAAGCFIRMYPLQKEKGFLLQDDFCDDLTDDLDLVFLCNPNNPTGLTIPQELLLK
                     ILDNCRERNIYLVLDECFIEFLPEPEKVTMQGKLDEYPNLLILRAFTKIYAMPGLRLG
                     YLLCSDEDLLDRISRSMQSWNVSIPAQMAGLAALNEDAYVKEARELIGKERAWMKAEL
                     EKAGLTVWDSCANYLFFEGPKGLAARLEKGGILIRDCSNYPGLADGYYRAAVRTHEEN
                     QILTEAVSQILQWF"
BASE COUNT         1630 a         2170 c         1760 g         2351 t
ORIGIN      
        1 ctcgtaatgc tctcttttgc ggatttcctg ctgaatgcct gcttttgcac agctacgttt
       61 aaatctacgc agagcactat ctaaagtttc gttttctttt acgataacgt ttgacatagt
      121 ctcacaccta acctccctcc agttgtagat tgtgcataat acaactgtat gggtattttt
      181 tgcactgttc ttattatacc agaaaaatct cgtacgtcaa caagtttttt catttttttg
      241 caaaattttt ccctgccgcc tgcctggctc ctttttctgc cctgcaggcg gcaggatttt
      301 gtgctgtttt tcctgcttat tgttatgcga tctggccttc cagtacctcg gcagcttttg
      361 caactgcttc gtcgatcttc tccggattct ttccgccggc ctgtgccatg ttcggacgtc
      421 caccgccgcc gccgccgaca acggctgcca cttctttgat cagttttccg gcatgtgcac
      481 ctttcttcat cgcatcatcg gttaccatgg caagaaggct tactttgccg tcctttgcag
      541 atgccagaac aacaacgccg cttcccagtt tttctttcag ctggtcgcca aggtcacgca
      601 gtccgttcat atcgacatcc ggtacggatg cagcgagaag ttttacacct ttgacttcgg
      661 ttactttatc cataacatcg ccgagcgctt cttttgccag tttgctcttt aaggactcat
      721 tctctgcctg gagtgctttt gcctctgcct gcagatgagc gatcttctcg aggagatccg
      781 ccggtgttgt ctttaagaga gctgctgcct catgcatttt ctcttcctgt gctttatagt
      841 atgcagtaac agacggtccg gtcagagctt cgattctgcg gacacctgct gcaacgcctg
      901 cctcagaaac aagtttgaac agtccgattt cagcggtatt ctttacatgg gtaccaccgc
      961 agagctcttt ggagaagtct cccatggaaa cgacacgtac tttgtcgccg tatttctcac
     1021 cgaacagagc catcgcgccg gatttctttg cttcctcagt atccatgatg tcggttacaa
     1081 ccgggatctg ctcctgaatc ttttcattta ccagattctc aactttttcc agttcttccg
     1141 gtgtcatagc ctggaagtgg gcaaagtcga aacggagtct ggtcggggtt acgagagaac
     1201 ctttctgctc tacgtgtgca ccgagaacag tctttaatgc cttctgcaga aggtgtgttg
     1261 cgctgtggtt cttctcgata ttttttcttg cctcttcgtt gacctgaagt gtaacttcct
     1321 cgccgtttga aatcataccg gatttcatga caccgacgtg gccgtatttt ccgccgcgga
     1381 gtttgatggt atcttctact tcgaatacac catttgctcc tttgatgata ccgcagtcgc
     1441 cttcctgacc gcccatggta gcgtagaacg gagttttctc tacgaaaatg gttcccttct
     1501 ggccttccat cagagattct gcaatctcag tctctgtcgt cagaacggtt actttggagg
     1561 tatcctccag atgatcgtat ccggtaaact cagttgttac ggacgggtcg atctcatcgt
     1621 aaacggttgc atcagcaccc atgtagtttg ttacttcacg gctcgatctt gcacgctctc
     1681 tctgctcttc cattgctgca tggaaaccgt cctcgtcgat gccgtagccc ttctcctcga
     1741 ggatctcttt ggtcagatcc agcgggaatc cgtaggtatc gtacagtttg aatgcatcct
     1801 gtccgtccag aacttttttg ccttcgctct gcattttttc ttcgagctcg gagaggatat
     1861 gaagtccctg gtcgatggtt ttgttgaact gggtctcctc gttgttcaga acgttgaaga
     1921 tgaaatcttt tttctcttca agttccggat atccgtcttt ggagccttcg attacgcttg
     1981 cggaaaggcg ggcaaggaac agtccgtcaa tgccaagcag acggccatga cgtgctgcac
     2041 ggcggatcag acggcgcagt acgtagcctc ttccttcgtt ggtcggcatg attccatcgg
     2101 aaatcatgaa tgttgcggaa cggatatggt ctgtgataag gcggatggaa acatcggtct
     2161 catgatcttt atcatactct tttccggaga tttcgcatac tttgttgcgg agtgcctgaa
     2221 tggtgtcgac atcgaagatg gaatctacat cctgtacgac aacggccaga cgctcaagtc
     2281 ccataccggt atcaatgttc ttctggatca ggtcggtgta gtggccctcg ccgtcgttgt
     2341 tgaactggct gaatacgttg ttccatactt ccatgtaacg gtcacactcg cagccaacgg
     2401 tacatcccgg tttgccgcag ccgtattttt ctccgcggtc ataataaatc tcagaacacg
     2461 gaccgcacgg acctgcgcca tgctcccaga agttgtcctc ttttccaaaa cggaaaatac
     2521 ggtctgccgg gatgccgatc tctttgttcc agatatcaaa tgcttcatca tcgtcctgat
     2581 agatggatgg gtacagacga ttcggatcga gccctactac ctgtgttaaa aactcccagg
     2641 accatgcgat tgcttcgtgt ttgaaataat caccgaagga aaagtttccg agcatctcaa
     2701 agaaggtgcc gtgacgggct gtttttccta cattttcaat atcgccggta cggatacatt
     2761 tctggcatgt tgttactctt cttctcggcg gaatctctgc gcctgtgaag taaggtttca
     2821 gcggagccat accggagttg atcagtaaaa gacttttatc gttgtgtgga atcaacgaaa
     2881 aactcttcat agcaagatgt cctttgctct cgaagaactc aagaaacatc tttcgtaatt
     2941 cgtttacgcc atatttttcc atgactgttt gtcctccaaa taattcagtg tgtcccggaa
     3001 tttcttccgg gaatccattc ttttataata gcacacttaa ttatttaacg caaatttttt
     3061 tgaaattctt ctttcctttt ttcagaacga taccgtcctc gccgaaggct gctttttcta
     3121 cggcatattt cggatctgtg attttctcac cgtctacgga tacaccnnnn nnnnnngtct
     3181 ttgcaagctc tgccttgaca agaagggtta cgatatcgat tttatcgtcc gtgaaatcct
     3241 ctgcagaaat ttcactggtc ggcatatgct cggagcttcc ggtagagaat acggcacgtg
     3301 cgccttcctg cgctttcttt gcttcttcct cgccgtgtac catggaggtc agctcatagg
     3361 ccaggatctc ttttgcctcg ttcagcttgc ttccttccca ggaatccatc tcgtcgatct
     3421 gctccagcgg caggaaggtc agcatacgga tacattttaa gacatcggca tcgtccacgt
     3481 ttctccagta ctggtagaaa tcgaacgggc tggtcttgtt cgggtcaagc catacagcgt
     3541 ttcctgcggt tttacccatc ttcttgccct gagagtcggt cagaagggtg atggtcattg
     3601 cataggcatc ttttccaagt ttacggcgga tcagttcggt tcctcccagc atgttggacc
     3661 actggtcatc gccgccgaac tgcatattgc agccgtattt ctggaacatg tgatagaagt
     3721 cgtagctctg catgatcata tagttgaact ccaggaagga aagacctttt tccatacgct
     3781 gtttgtagca ctctgcacgg agcatgttgt tgacgctgaa gcatgcacct acctcacgga
     3841 gcagttctac atagttcagg ttcagcagcc agtctgcgtt gttgagcatc atagcttttc
     3901 cctcgccgaa ctcgatgaaa cgctccatct gacgtttgaa gcactctgcg ttgtggtcga
     3961 tctcctcgcg ggtcagcatt tttctcatat cggttcttcc ggacgggtca ccgatcatgg
     4021 tggtgccgcc gccgatcagg gcgatcggtt tgtttcctgc catctgcatc cgtttcatca
     4081 gggtcagtgc catgaagtga cctgcggtca gactgtctgc ggtgcagtcg aatccgatat
     4141 agaacgttgc ttttccattg tttaccaggt ctctgatttc atcttcgtcg gttacctgtg
     4201 ccagaaggcc tctggcgatc agttcgtcat aaatctgcat tttgtttttc tccttttttc
     4261 ggttgacttt cgggtatgcg ttaccgtctg ggaccaaagt tttttataca aaaagactcc
     4321 cgtcctttcc gattcaaagg acgagagccc gtatctcccg tgttaccacc ttacttcaca
     4381 gctgtctcac ggcagctgcc tttgtcggta tccaaaacct ctgaataccc tgacgctgta
     4441 acgttcgtct cttacgtcgc agcctactaa aaacgtgcat catagttctt ctgccgcccg
     4501 ttttattcgg tgcgcaactc cgggatgtat tcgaatcgta cagaacctgc atgcgcctct
     4561 catctgccgg ctgctttctg tccgcgtttt tgtacgtcta cttgttccct tccaagcctt
     4621 tttccatgtt ccatagtata aacggtgatt cgaaatctgt caagacttct gctttccgtt
     4681 ttttcatcta tttttaatat ccgcggtcct gatccatcat atacaggagt gcgttgcaga
     4741 tgcacgccgc gatattgctg ccgccttttc gtccgcgtgc cacgatatac gggatattgg
     4801 tctgcataat cagttctttg gactgtacca cattgacaaa gccgaccgga actccgatga
     4861 tcagcgccgg ttttattttt ccttccccga tcagttcata gaggcggacc agtgccgtcg
     4921 gcgcatttcc aaccgcaata atcagctttt tatccagctc cgccgctttt tccatgcagg
     4981 ctgccgcgcg ggtgcttccg gtttcttttg ctcttgccgc aacatcttcg tctgacataa
     5041 agcagagcgc ttcaccgccg tggcgcgcaa gcaccttttt gttgatgccg gatcttgcca
     5101 tctgcgtatc cgtcacaatg caggctccct cccggatcgc ctcgatgcct tttgccaccg
     5161 catgctccga aaagcagaga ttatccgcat aatcaaaatc cgcacttgtg tggatacacc
     5221 gctttacaac cagttcctga tcctctggaa acgttcttcc gtttaattct tccgtgataa
     5281 tttcaaagct tctcttttca atctcgtgcg ggcttacctg ctccagttct atcttcatct
     5341 ttatgcccct tcttccagaa tttgataaat cttcttcatg tcgagatgct cgcgcagcgc
     5401 atctgcgaga atatcgtact gtgtctcttt gaatgtctgg taatcgacac cgtgaatctg
     5461 ctccatggca attcccttat tctttgcaag tgcttccacg atccgctcgg caacttcttc
     5521 tttgtcgaaa atgccgtgca catacgtacc gtacaccgtc cagcttatcc gaatcagcac
     5581 ggttctgatc atcgatgaac gtgaatgctt ctgttccttc tttccgcacc gtttctccca
     5641 tatggatttc atagccctcg atccgggttc cctcgatccc ggcaagaacg ccttctgttt
     5701 ttccaaaagc gccctccgcg cgggttctcg tcttctcacc ggcaaatacg gtatccatcg
     5761 gcaggagtcc cattcctttg atcgttccgc cggcttcgac atgcagcgga tcgctcagcg
     5821 tatctccgag catctggaag ccgccgcaga caccgaaaat tacttttccg gccgcagccg
     5881 ccttcagtac ggccgcttcc agaccattct gacgcatcca gagcagatcc tccatcgtgt
     5941 ttttcgttcc cggaagcaga atcatatccg gatttttcag ttcggagaca gagcttacgt
     6001 agcggagtga tacgccttcg atccgctcga acgggttgaa atcggtgaag tttgaaatac
     6061 gcgggagacg gatgaccgca aggtcaatga gaccgatctc ctcttttctg gtgaatcgct
     6121 cggtcagact gagatacggc gccacgccga ccaccgggat gtgtgttttt tcttccagca
     6181 tcacaacgcc cgggtccagg atggttttgt ccccgcggaa tttgttgata atcagaccct
     6241 tgatgcggtc gcgctcatcc ggctctaaaa gctctacggt accgtaaagc tgagcaaaaa
     6301 cgccgccgcg gtcaatatct ccgacgagga gcaccggtgc atccacaagc tttgccattc
     6361 ccatattgac gatatcatcg gctttcaggt tgatctccgc cggacttccg gctccctcga
     6421 tcacaatgat atcatactgt tcttccagtg catggtacgc tttcatgatg tccgggatca
     6481 gctctttttt gtgggaaaaa tattcccgcg cactcatgtt tcccagtacc tcaccgttta
     6541 caatcacctg cgaaccggtg tcattcgtcg gctttaacag aatcgggttc atcaaaacgg
     6601 acggttcgat cccggccgct tccgcctgca tgacctgtgc acgtcccatc tcaaggcctt
     6661 ctctcgtaat aaacgaattc agcgccatat tctgtgattt gaacggcgcc acacggtatc
     6721 cgtcctgatg gaaaatccgg cataagcccg ccgcaagcag actttttccc gcatttgaca
     6781 tcgttccctg tatcataatt actttcgcca tcgtttttcc tcctaaaacc actgcagaat
     6841 ctgtgaaacg gcttctgtca gaatctggtt ttcttcatgt gtgcgtacgg ctgcacggta
     6901 atatccgtcg gcaaggcccg ggtagttgga acagtcgcgg atcagaatgc cgcctttttc
     6961 cagccgtgcg gcaagtcctt tcggtccctc aaagaacaga taatttgcac aggaatccca
     7021 tacggtaaga cctgcttttt ccagttctgc tttcatccag gcgcgttctt ttccgatcag
     7081 ttcccgtgct tctttgacgt aagcatcctc gttcagggcg gcaaggcctg ccatctgcgc
     7141 cggaatcgaa acattccacg actgcatgct gcggctgatc cgatccagca gatcctcgtc
     7201 gctgcagagc agatagccaa gacgcaggcc cggcattgcg taaatctttg tgaacgcacg
     7261 cagaatcagc agattcggat attcgtccag ttttccctgc atcgtcactt tttccggctc
     7321 cggcagaaat tctatgaaac actcatccag caccagatag atgttacgct cccggcagtt
     7381 atccaggatc tttaaaagaa gctcctgcgg aatcgtaagg ccggttggat tatttgggtt
     7441 acataaaaag acaagatcca gatcgtcggt cagatcatcg cagaaatcgt cctgtaaaag
     7501 aaagcctttt tccttttgca gcggatacat acggataaaa caccctgccg ctgaaagtgc
     7561 tgcttcatac tctgcaaatc ccggcgctgc aatcagcgct tttttcgggc ggagagcctg
     7621 tgtcagggca aaaataagct ccgcggcgcc gtttgcaaac aggatatttt cttctggaag
     7681 ctgctccttt tccgacagtg cccgggtcag gtcccggcac agtgcgtcag gatactgttc
     7741 caccgattcc gcgctctttc tcaccgcctc tttcacactt tccggagctc caagaggatt
     7801 gacattcaca gaaaaatcga ctctgcattt tttcgtgtaa atgtctcctc catgtggatt
     7861 ttgtctcata ccatatgcct tcctttctct tttccgttgt gtttctatct tctcttttta
     7921 c
//
LOCUS       DAOMUF010000097         7840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2414_length_7840_cov_4.025434, whole genome shotgun sequence.
ACCESSION   DAOMUF010000097 DAOMUF010000000
VERSION     DAOMUF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_7840_cov_4.025434"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1036)
                     /locus_tag="PLN77_06695"
     CDS             complement(44..1036)
                     /locus_tag="PLN77_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM43999.1"
                     /translation="MKKAVKAILNVLSYGGIEVESARRLADIKKLDPIRIFLRKLDME
                     IYNGDYKVPIRLFFPDEKMEEHGAKEAILFFHGGGWTTESVETYDRVCARMAQSTGQI
                     VVSVEYRLAPEHRFPTGLEDCYAAAQAMLRGEILPEVTPENVTIMGDSAGGNLAAAVC
                     LMARDKKELLPGKQILIYPAVNSCYTEKSPYKSVKTNGTGYLLTSVKMEDYVILYQRT
                     EEDRKNPYFAPVLAENLQGLPDTLVLTAQYDPLRDEGEDYAKKLKRAGNLVEHHRIKG
                     ALHGYFALGIEHLYVQESFQYMNAFLRKEKQQKNADEELENIAAVKKQLTHGKA"
     gene            complement(1055..2173)
                     /locus_tag="PLN77_06700"
     CDS             complement(1055..2173)
                     /locus_tag="PLN77_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRM44000.1"
                     /translation="MNKNEIIQIQGMEYKEMTKQLLNKCALATLIPSKVSLIGIKPNL
                     VAPVPAEFGGTTHPEVVAGIIEYLQENGFTNLQIMEGSWVGDKTEESFEVCGYRMLSE
                     QYGVPLIDAQKEKSMPVQCGDMELQICECAKKVDFMINVPVMKGHCQTKITCALKNMK
                     GLLPNKEKRHFHAMGLHRPIAHLGLGIHQDFILVDNICGDLDFEDGGNPFIMNRLFAG
                     LDPVLIDAYVCAELHYKPEDVPYVKMAEELGVGSADLTRLSIRQIGEIGEKRVIPEKR
                     KIVELQDVVEEVESCSACYGYLIPALDRLKEEGLLQELHEKICIGQGYRGKSGALGVG
                     SCTSGFACNLKGCPPTDEQMYEFLKQYIATRRKTEAEK"
     gene            2420..3772
                     /locus_tag="PLN77_06705"
     CDS             2420..3772
                     /locus_tag="PLN77_06705"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HRM44001.1"
                     /translation="MSFEDDLKDVGDSIGKLVDNAVNSQNFQELNRKISDTINQFVYP
                     NRKGPFRANGTQEPSRGYSADTSYSSQHSSHNNTAQQTGPHHYSYGPGGTMPGKSANP
                     VPKPSHTWQTPQNTEWNSRIQERRVLRERFMPSTDLALVGGFQTAAGWGVTGLFGCCT
                     ALSLYSLFAGPDNSALIPSLIFAAFTIGGGILLKKGSKNRRLVRHFRQICTLIGTKEY
                     ISTKELCDSMHCEKGELLTDITTMIDKSMLRQGHLDENGTCLMVTKDCYDQYRAMLAS
                     QKEQQIAEQKAREQLEASGVTPEYQQMLEECESYIQKIHQCNLDLPGEVITAKLSRLE
                     TVVTRILEEAKKRPKEAAALRKFMDYYMPTTWKLLDAYRSFENEPIQSDNILRTKKEI
                     EDTLDTINAAFEKLLDDLFQTTAWDISSDISVLQTMLAQEGLTNQAGPSKQDIEPLHM
                     "
     gene            3846..4994
                     /locus_tag="PLN77_06710"
     CDS             3846..4994
                     /locus_tag="PLN77_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HRM44002.1"
                     /translation="MNDFVKDTTEIPSLSFGAPEPEPELPVQQPEEKKEVMDDSVLSD
                     EQKAMVDAFVSQIDLANTNGILQYGAGAQKKMADFSEKTLENVRSKDLGEIGDLLTSV
                     VTELKTFDAEEEKGPFSFFKKSTNKINAMKAKYDKAEVNVNKICDVLDGHQRQLLKDI
                     AILDQMYAQNLNYFKELTMYILAGKKKLELVRSTELRDLQNKAQMTGKAEDAQAAKDL
                     EDLCNRFEKKLYDLELTRMVSIQTAPQIRMIQNSDSVMAEKIQSTLVNTIPLWKNQMI
                     IALGVHHSTEAAKAQRQVSDMTNELLKKNAENLKMATIETAKESERGIVDMETLKQTN
                     QSLISTLDEVMKIQEDGRQKRAAAEVELVQLENDIKKKMLELSAGGQH"
     gene            complement(5076..5588)
                     /locus_tag="PLN77_06715"
     CDS             complement(5076..5588)
                     /locus_tag="PLN77_06715"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRM44003.1"
                     /translation="MKAILSADRKWGLGYQGRLLVSIPSDLRFFRETTMGHVVVMGRK
                     TLATLPAGQPLKGRTNIILSRDPEWTVRGATVVHSIKELMEVLKPYEDDDIYVAGGGE
                     IYRQLLPYCDTAYVTRIDMTYQADTFFEDLDESPDWEMVEEGDEQTCFDIEFYFTEYR
                     RKNEKAERPR"
     gene            complement(5585..6418)
                     /gene="larE"
                     /locus_tag="PLN77_06720"
     CDS             complement(5585..6418)
                     /gene="larE"
                     /locus_tag="PLN77_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRM44004.1"
                     /translation="MQNKEKALLTQLDEIAKQDLCLAFSGGVDSSLLLKLVMDASAKY
                     GTKVYAVTFQTRLHPPCDLETATRVAKEVGAVHEVLFVDELEQEAIRYNPENRCYLCK
                     HYLFGKLLEFARDHGVTQVLDGTNEDDLHVYRPGRKALREYGVISPLAACHVTKTEVK
                     ALAAKYGVSVAHRPSTPCMATRLPYGAEINYDVLDRIADGEAWLHTLFGAEENLRLRV
                     HGDVVRLEIAPERMGEVLEKREEMIAYLKKLGFSYLTMDLEGFRSGSMDEKITQKEET
                     K"
     gene            complement(6578..>7840)
                     /locus_tag="PLN77_06725"
     CDS             complement(6578..>7840)
                     /locus_tag="PLN77_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRM44005.1"
                     /translation="GKLLTNSWLDYNGNSYYLKADGKMAFNEWLDNTYYFRSWGAAYK
                     NAWAKVNNVWYYFDGNGKKYTSGWLTYKGNKYYLKSDGTMLANEWLDGKYYFKSWGGM
                     YKNEWGKSGDTWYWFNADGTKRTQKGWFLYDKNYYYLDKDGKMLTGWVYHDGNYYYMK
                     SWGGMAHDEWILHDKNWYYFKSWGGMYHDQWLTLNGSQYYFRSWGGRYQNCTATINGK
                     QYKFDASGRRITEGWEYIGKYRRYRKADGSLMEDVTSIFNPSSKYITVDRTRGRVTIY
                     GYNSATGSYDTPIKSMICSVGNPISYTAAGTYKIGWQLKKKQMRGEDYVCWAPYVSQI
                     YGAVYFHGVASSTPDLNMISAVDFNSLGSPMSHGCVRLTAIDAKWIYDNVSSGTTVRI
                     GDNLDYPLTNPTRYTWTGGAFGSDPTYR"
BASE COUNT         1967 a         2044 c         1703 g         2126 t
ORIGIN      
        1 tctccagcgc gaagaatgtt ctttcttcat tttctatgcc tccttacgcc ttcccgtgtg
       61 tcaattgttt cttcactgct gctatgtttt ccagttcttc gtctgcattt ttctgctgtt
      121 tttctttcct taaaaatgca ttcatatact gaaaactttc ctgcacatat aggtgttcaa
      181 tgccaagcgc aaaatagccg tgcaatgcgc ccttgatccg atgatgttca accagattgc
      241 cagccctttt cagctttttc gcataatcct cgccctcatc gcgcagcgga tcgtactgcg
      301 ccgtcagtac cagcgtgtcc ggcagtccct gcagattttc tgccagtacc ggtgcaaagt
      361 acggattttt gcggtcttct tcggtccgct gatacagaat tacataatct tccattttga
      421 cactggtcag aaggtatccg gttccattgg ttttcacgga tttgtacggt gatttctctg
      481 tgtaacagga attcaccgcc ggataaatca aaatctgttt ccccggcagc agttcttttt
      541 tgtccctcgc catcaggcag accgcagccg caagatttcc tcctgcgctg tcgcccatga
      601 tcgtgacatt ttccggcgtt acctccggca gaatctcccc gcgcagcatt gcctgggcgg
      661 cggcgtagca gtcttctaag cctgtcggga aacgatgctc cggtgcaaga cggtattcta
      721 ccgatacaac aatctgcccc gtcgactgcg ccatgcgtgc gcagacacgg tcgtatgtct
      781 ccacactctc ggttgtccag ccgccgccat ggaagaacag aatcgcttct tttgcaccat
      841 gctcctccat tttttcatcc gggaagaaca gccgaatcgg aaccttgtag tccccattat
      901 atatttccat atccaatttt cgcagaaaaa tacggatcgg atccagcttc ttgatatcgg
      961 caagccgacg ggcggattct acttcaattc cgccgtagga caatacattt aaaatggctt
     1021 tgactgcttt tttcattcgc tctctcctct gttgctattt ttctgcttct gtttttcgtc
     1081 tagttgcaat gtactgcttc aaaaattcat acatctgctc atccgttggc ggacagcctt
     1141 tcagattgca tgcgaacccg gaagtacaac ttcccacacc aagcgccccg ctctttcccc
     1201 ggtatccctg cccgatgcag attttttcgt ggagttcctg cagcagtcct tcttccttca
     1261 gccggtcaag cgccgggatc agatagccgt agcaggcact gcaggactca acttcctcta
     1321 cgacatcctg caattccacg atcttccgtt tttccggaat cactcttttt tcaccaattt
     1381 cgccaatttg gcggattgaa agccgtgtga gatcagcgct gcccacgccc aactcttctg
     1441 ccattttgac atacgggaca tcttctggct tataatgcag ttccgcgcag acatatgcat
     1501 cgatcaacac cggatctaac ccagcgaaaa ggcgattcat aataaacgga ttaccgccat
     1561 cctcaaagtc aaggtctccg cagatattat ccaccaaaat aaaatcctga tgaatcccca
     1621 gccccagatg cgcaattggc cgatgaagtc ccatggcatg aaaatgacgt ttttctttat
     1681 tcggcaagag acctttcatg tttttcagtg cgcaggtaat tttagtctgg caatggcctt
     1741 tcatgaccgg gacattgatc ataaaatcca cctttttcgc acattcgcaa atttgaagtt
     1801 ccatatcacc acactgcacc ggcatgcttt tttccttctg cgcatcgata agcggaacgc
     1861 cgtactgttc cgaaagcata cggtatccac agacctcaaa cgattcctcc gtcttatctc
     1921 cgacccaaga gccttccatg atttgaaggt tagtaaatcc gttttcctga agatattcga
     1981 tgatccccgc aaccacctcc ggatgtgtcg tccctccaaa ctccgccggg acaggtgcta
     2041 ccagatttgg cttaataccg atgagactga ccttcgaagg aatgagcgta gctaaggcac
     2101 atttgttcaa aagctgtttt gtcatttctt tatattccat tccctgtatc tggataattt
     2161 cgtttttatt catttggtat tgcggcccct ttattctaat ttgatttttt ctgacattcg
     2221 atattcttta gtatataata gttactgctt tctttcaact catccaaaaa caaaatcatt
     2281 tcctgtgaac tgtaaccatt cccatcatct ccagcttcat agaaaattaa gcactgtatg
     2341 cttgtaagat ttccaatcgc attgtataat aactggaaga cggtacacag aaaaagtacc
     2401 tcatcagaaa ggaactgaca tgagttttga agatgattta aaggatgtcg gcgacagtat
     2461 tggaaagctt gtggataatg ctgtaaattc tcagaatttt caggaattaa accggaagat
     2521 ttcagacacc atcaaccagt ttgtctaccc aaaccggaag ggaccattcc gtgcaaatgg
     2581 cacacaggag ccgtcccgcg gctattccgc agatacttcc tacagcagcc agcattcttc
     2641 ccataataat acagcgcagc agaccggacc gcatcactat tcctacgggc cgggaggcac
     2701 gatgccgggt aagagcgcga acccggttcc caaaccttcc catacctggc agacccccca
     2761 gaatacggaa tggaacagcc gcatacagga acgccgtgta ctgcgcgaac gctttatgcc
     2821 ctccaccgat cttgcacttg tcggcggatt tcagacggct gccggctggg gtgtcaccgg
     2881 actgttcggc tgctgcacag cgcttagtct gtattcctta tttgcaggtc cagacaacag
     2941 cgcgctgatt ccttctctta tttttgccgc atttaccatc ggcggcggca ttcttctgaa
     3001 aaagggaagt aaaaaccggc gtctggtccg ccatttccgc cagatctgta cgctgatcgg
     3061 cacaaaggaa tatatttcta cgaaagaact ctgcgacagt atgcactgtg agaaaggtga
     3121 gcttctgacc gatattacaa ccatgattga taaatccatg ctgcgccagg gccacctcga
     3181 cgagaatggt acctgtctga tggtgacaaa ggactgctac gaccagtacc gcgcaatgct
     3241 cgccagccag aaggaacagc agattgccga acagaaagca cgtgaacagc tggaggcctc
     3301 cggtgtaacg ccggaatacc agcagatgct ggaagaatgt gaatcttata ttcagaagat
     3361 ccatcagtgc aacctggatc tgccgggtga agtcatcaca gccaagctga gccgtctcga
     3421 aacggttgtt acccggattc tcgaggaggc aaaaaagcgc ccaaaagaag ccgccgccct
     3481 gcggaaattt atggattact atatgccgac aacctggaag ctgctcgacg cttaccgttc
     3541 ctttgagaac gaaccaattc aaagcgacaa tattctccga accaagaagg aaatcgagga
     3601 cacactggat accatcaatg ccgcattcga aaagctgctt gatgatctgt tccagacaac
     3661 ggcatgggat atttcttctg atatctctgt tctgcagacg atgctggcgc aggagggact
     3721 gacaaatcag gcaggtccgt caaagcagga tattgagccg cttcacatgt aactattcaa
     3781 agctatacaa ttgaaatcaa atcaccggtt acgtttccgt accgaagaaa aggagaatag
     3841 aaaacatgaa tgattttgta aaggacacca ctgaaattcc aagcttaagt ttcggcgctc
     3901 cggagccgga accggaactt ccggtacagc agccggaaga aaagaaagag gtcatggatg
     3961 attctgttct gtccgacgaa cagaaagcca tggtggatgc ctttgtttcc cagattgatc
     4021 ttgccaatac aaacggcatt ctgcagtacg gtgccggtgc acagaagaaa atggcagact
     4081 tttccgagaa aacgctggag aacgtcagaa gcaaagatct cggggaaatc ggcgatctgc
     4141 tcacctctgt tgtgacagag ctgaaaacgt ttgacgcaga agaagaaaag ggaccattct
     4201 ctttctttaa gaaaagcacg aacaaaatca atgcaatgaa agcgaagtat gacaaggcag
     4261 aggtgaatgt caataaaatc tgtgacgtgc tcgacggcca ccagcggcag cttctgaaag
     4321 acattgccat tctggatcag atgtatgcac agaacctgaa ttattttaaa gagctgacca
     4381 tgtacattct cgctggaaag aaaaagctgg agctggtacg ttccacagaa ctccgtgatc
     4441 tgcagaataa agcgcagatg accggaaagg ccgaagatgc acaggctgcc aaggatctcg
     4501 aagatctctg caaccgattt gagaagaaac tgtatgatct ggaactgacc cgtatggttt
     4561 cgatccagac agcgccgcag atccgcatga ttcagaacag cgattctgta atggccgaaa
     4621 agatccaatc cacactcgtc aacaccattc cgctctggaa aaaccagatg atcattgcac
     4681 tcggcgttca tcactcgact gaggctgcca aagcacagcg ccaggtttcg gatatgacaa
     4741 atgagcttct gaaaaagaat gcggagaatc tgaagatggc aacgatcgag actgcaaaag
     4801 aatctgagcg cggtatcgtc gatatggaaa ccttaaaaca gacgaaccag agcctgatct
     4861 ctaccctcga tgaagtgatg aagattcagg aggacggccg ccagaaacgt gccgccgctg
     4921 aagttgagct ggtacagctc gaaaatgata ttaagaaaaa aatgctggag ctttctgcag
     4981 gcggacagca ctgatacttt ttgtatccta tatgaaaaaa aacggagaag ccgctttttc
     5041 tgtggtttct ccgtttttta cgtcctgtct tacccttatc tcgggcgttc tgccttttca
     5101 ttctttcttc ggtattccgt gaaataaaat tcaatatcaa agcaggtctg ctcatcgccc
     5161 tcttctacca tctcccaatc cggtgattcg tcaagatcct caaagaaggt atccgcctga
     5221 tacgtcatgt caattcgtgt gacgtatgcc gtatcacagt atgggagaag ctgacggtaa
     5281 atttctccgc cgcccgcaac ataaatatcg tcgtcctcat acggctttaa cacttccatc
     5341 agctctttga tactgtggac aacggttgcg ccgcggacag tccattccgg atcgcgactt
     5401 aagataatat tcgttctgcc ttttaagggc tgccctgccg ggagtgtagc aagtgtcttt
     5461 cttcccatca cgacaacgtg tcccatcgta gtttcacgga agaatcgcag atcagacgga
     5521 atacttacca gcagccgtcc ctgatatccc agaccccatt tgcggtccgc acttaaaatt
     5581 gctttcattt tgtttcctct ttctgtgtta ttttctcatc catactgccg gaacggaacc
     5641 cttccagatc catcgtcagg taagaaaagc cgagcttttt cagatatgca atcatttctt
     5701 ctctcttctc aagcacttcc cccatacgct ccggtgcgat ttcaagacgc accacatcgc
     5761 catgtacacg caggcggaga ttctcttccg caccgaacag cgtatgaagc catgcttcgc
     5821 cgtccgcaat ccgatccaga acgtcatagt taatttctgc cccgtaggga aggcgtgtcg
     5881 ccatacatgg agtggatgga cggtgtgcta cggaaacgcc gtactttgca gcaagtgctt
     5941 ttacctctgt ttttgtcaca tgacacgctg caagcgggct gatgacacca tattctctca
     6001 gcgcttttcg ccccggccgg tagacatgaa ggtcatcctc atttgttccg tcgagtacct
     6061 gggtgacacc gtgatcgcgg gcaaattcca gaagtttccc aaatagatag tgcttacaga
     6121 gatagcagcg gttttccgga ttgtaacgga ttgcctcctg ctccagctca tcaacaaaaa
     6181 ggacctcatg aacagcgcca acctcctttg ctactcttgt cgctgtctcc aggtcgcagg
     6241 gcggatgcag ccgtgtctga aaggttactg cataaacctt tgttccatat tttgcagatg
     6301 catccatgac aagcttcagc agcagactgc tgtccacacc gccggagaat gccagacaga
     6361 gatcctgttt tgcaatctcg tcaagttggg taagcaatgc tttttcctta ttctgcatat
     6421 ctgttttctg ttctgtccgg ttctttttct tttcctgtaa ttccatcgta tctgtcttcc
     6481 tttcctgtaa aatggccgat gtcttcttat cagaaaatcc atcggcggca ctgctgcccc
     6541 gatggatttt ctcatacagg tattcctatt ctatttttta tcgatatgtc ggatcgcttc
     6601 cgaaagcacc gcctgtccag gtataacggg tcgggtttgt cagcggataa tccagattgt
     6661 cgccaatgcg gaccgttgtt ccgctgctga cgttatcata aatccatttt gcatcgattg
     6721 cagtcagtct gacacaacca tgtgacatcg gtgatcccag ggaattgaag tctacggcag
     6781 agatcatatt cagatccggt gtgctgcttg ctacgccatg gaagtaaaca gcgccataaa
     6841 tctgtgatac atatggtgcc cagcacacat aatcttcacc tctcatttgc ttctttttca
     6901 actgccagcc gattttatat gttcctgctg cggtgtaact gattggattt cctacggagc
     6961 agatcattga tttgatcggg gtatcgtaag aaccggttgc actgttatag ccgtaaatgg
     7021 tgacacgtcc gcgggttctg tctactgtga tgtatttgga tgacggattg aagatgcttg
     7081 tcacatcctc catcagagaa ccgtctgctt tgcggtaacg gcggtatttt ccgatgtact
     7141 cccatccttc cgtgattctg cgtccggaag catcgaattt gtactgtttt ccattgatcg
     7201 tagcggtgca gttctgatat cttccgcccc agctgcggaa ataatactga gaaccgttca
     7261 gtgtcagcca ctgatcatgg tacatgccgc cccagctctt gaagtagtac cagttcttat
     7321 catgaagaat ccactcgtca tgtgccatgc cgccccagct cttcatgtaa taatagttac
     7381 cgtcatggta aacccagccg gtcagcattt ttccatcttt atccagataa taataatttt
     7441 tatcatagag gaaccagcct ttttgcgtcc gtttggttcc atctgcatta aaccagtacc
     7501 aggtatcgcc ggacttgccc cattcgtttt tatacatacc accccagctc ttgaagtagt
     7561 attttccatc gagccattca ttggcaagca tggttccatc ggatttcaga taatacttat
     7621 ttcctttgta ggtcagccag ccgctggtat atttctttcc atttccgtca aaataatacc
     7681 atacattgtt gacttttgcc catgcgtttt tgtaggccgc accccagctg cggaagtaat
     7741 aggtgttatc cagccactca ttaaaagcca tttttccatc ggctttcaga taatagctgt
     7801 ttccattgta atccagccag gaattcgtca gcagttttcc
//
LOCUS       DAOMUF010000098         7806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2430_length_7806_cov_4.802348, whole genome shotgun sequence.
ACCESSION   DAOMUF010000098 DAOMUF010000000
VERSION     DAOMUF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2430_length_7806_cov_4.802348"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLN77_06730"
     CDS             complement(<1..497)
                     /locus_tag="PLN77_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HRM44006.1"
                     /translation="MQIYVDGNAVRSGNGQKEYPFQTISEAAKIARPGDEVLVAPGVY
                     REYVDPANAGCEDARIVYRSVEPGKAVITGAEIVDNWEHLEGDVWTARVSNGLFGDYN
                     PYTTLVSGDWFIASYTAHTGEVYLNGKSMYEVTSLDQVKKPEIYKKSWDQAFTVYTWY
                     VEQDE"
     gene            743..1615
                     /locus_tag="PLN77_06735"
     CDS             743..1615
                     /locus_tag="PLN77_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM44007.1"
                     /translation="MKNDSTSFFENNHFANSYVYDISISHVPAYFGLHWHHYIEIVLA
                     QDDGIIYEVNGETFPLKPGDLLFIWPGELHAVSTTNREHRLIMLQLDADFLEKRVDFQ
                     SVAYLFYQIRLLNQNNFPEIEKLRGFLTELRRLSLSGISFSELRGCVLLYQFFILLGE
                     NLSVPTETAAPQKTGHSAQVHRQMVAACTWISSHCTEPVSLEEASKVAGFSRCHFSKL
                     FRAYTGLSFTDFVTRERLHIAENLLKKPDLTITEVSLESGFNSISTFNRVFLKSKNVS
                     PTDFRQMYLQSYQK"
     gene            complement(1617..2729)
                     /locus_tag="PLN77_06740"
     CDS             complement(1617..2729)
                     /locus_tag="PLN77_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM44008.1"
                     /translation="MGTTETKKMRVGVVGCGAISDIYLTNMIHNFENLEVAACCAGHL
                     EHAKKKAEQYGIKGCTYEEILADPSIEMVVILTPAPTHEGLIRQALEAGKHVYTEKTM
                     TLAPETAAKLIELAEEKHLYLGSAPDTFLGSALQTARKAIDDGLIGEVTSFEACANRD
                     LDLLASIFRFLRMPGGGICYDFGVYYLTAIVSLFGPVDRVVSSVRNLAPKRVNVVPDS
                     PEYGQEFDYSNESQAFTILEMKNGVTGTFCVNGDSTINDQAVFTIYGKKGILKLVDPN
                     NFGGDIVYIPGVKDWTQQAVPEVLDYGFAYSENSRGLGPSEMAEAIAEGRLNRANAKM
                     AYHVLDTIDQIMKSAETGAFEKVPSTCERPEAMPNS"
     gene            complement(2913..7178)
                     /locus_tag="PLN77_06745"
     CDS             complement(2913..7178)
                     /locus_tag="PLN77_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRM44009.1"
                     /translation="MRAFRKDFIREITKNKGRFLSVFFIVLLGAAFFSGIRSAEGDMK
                     VSADRYYDEVNYMDLKVLGTLGLTDDDLADIAKTDGVKAVYGGKTVEVLHDIGESEQV
                     VKLIALTDGVNEPRVVEGRMPEKEDEILVDTQFLKSSGCEIGDQVTFTSGTEDPLSDS
                     LTGDTFTIVGSATLPYYMDLNRGTGSIGNGSINSFALLLPETFTSDLYTEIYVQADGA
                     QEEASYSDAYDETVKAVQTKIEALEDAACDRRYTAVKTEGQEKIDDAKQQVADAEQKL
                     ADAKTELDDGAQQLADAKVTIADKEQELLDGEQTLKDKEQELLDGKQTIAEKEQELLD
                     GKQTIADKEQELLSAKATIADKEQELASGKATLKDKEAELASGKATLEAKAAELESGK
                     AELNQKADELASGKTTLEAKAKELSDGKAQLAEKETELASGKKELEEKMTQLSAAKTE
                     LTRKQTELNTAKEQLSEKETELNIAKEQLSAAREELDNKKAETAAGRTQYEAQKAAYE
                     EQKSQYETAKDQLAQISRQLPMVEAAQTEVTGQIEVIEAQLDGLTEEDEAYVSLLEQK
                     TALEAKQTELAQQLFTMQEQKTFLEQNIAAFEAASADAEARLAAAEAQITDAESQLAA
                     ADAQLTEKEQECAAGEAQIASAKEELESGEAQITEALAQLQDGEAQAEAYQKQIEDGE
                     AQLASAKAAIADGEAQIETNRSKLKDGETQLAAARAQLADGEQQIASYRQTIQSGEAQ
                     LAAAKQTISSGESQLADAKQTIADGETQLAEAKQTIADGESQLAEAKQTIADGETQLA
                     DAKQEIADGKISLADAKQEIADKEKELEDGKAEYEKAKADAEPEIADAKQEIADGEKT
                     LADLKKPTWYVWGRNKVTSTESFGQDAGRISNIGKFFPVIFFLVAALVSLTTMTRMIE
                     EQRQQIGTLKALGYSDGVIAFKYFAYAMLSAVSGALAGVVVGEKILPWVIMNAYGMLY
                     TGLPYYMTPLNWEQGGLAILASAACTGVATIAACYKELAAGPAELMRPEAPKNGKRIF
                     LERIGVLWKHLNFTQKSTVRNLVRYKKRFFMTVIGIGGCMGLILVGFGLQDSITAIAK
                     NQFVSLFTYQANAVLNSDVDESEKEALQTDLENYSGIDELLEMYCQNIELQTDKKTVD
                     AVLEVPKELTNFNDFYAFRDRKSGEVYEFPTDGGAAISEKTATMLGVKAGDTVQLKKG
                     DDIVDVKISIIVENYVRHYLYLAPDLYEELFGGAPDYNQLLMKYQDTSGNYETALGEK
                     IMTYDGVAAISFTSDLIDQIDNMLRSLDIVIVVLIVSAGLLAFVVLYNLNNINITERQ
                     RELATLKVLGFFDGEVASYVYRENMVLTLFGVIAGMGIGTFLHHCVIQTVEVDMMMFG
                     RNVFPRSYGWSALITLAFALFVNFMMFYRLRKIDMIESLKSVE"
     gene            complement(7186..>7806)
                     /locus_tag="PLN77_06750"
     CDS             complement(7186..>7806)
                     /locus_tag="PLN77_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44010.1"
                     /translation="FDVEKGEFVIVVGPSGAGKTTVLNILGGMDTATSGKVLVDGAEI
                     SKYRGKKLIQYRREDIGFVFQFYNLMQNLTALENVELAMQICKHPLDASEVLAEVGLE
                     ERMNNFPAQLSGGEQQRVAIARALAKNPKLLLCDEPTGALDYVTGKAILKLLQDTCRE
                     KGMTVIVITHNSALTPMADRVIKIRNGKVAGMKTNENPTPVEEIEW"
BASE COUNT         1584 a         2269 c         1689 g         2264 t
ORIGIN      
        1 tcttcgtcct gctccacata ccaggtgtaa accgtgaatg cctgatccca ggatttttta
       61 tagatctccg gctttttcac ctgatccaga cttgtcacct catacatgga ttttccattc
      121 agatacactt ctccggtgtg tgccgtgtag gatgcgataa accagtcacc gctgaccagt
      181 gtcgtatatg gattgtaatc cccaaacaat ccgttcgaaa cgcgcgctgt ccacacatca
      241 ccttccaaat gctcccagtt atccacaatt tccgcaccgg taatcacagc ttttcccggt
      301 tccacagaac ggtacacaat tcttgcatcc tcacatcccg cattcgccgg atctacatat
      361 tcccgatata cgccaggcgc tacaagcacc tcgtcgccgg gccttgcaat ttttgccgcc
      421 tcggaaatcg tctgaaatgg atattccttc tgcccatttc cgcttcgcac agcatttcca
      481 tctacataaa tctgcatttt tcgcttacct ctcacttctt cacttttcat aaacttttat
      541 tgtgaatttt cgccaaaatc acatcactct ttttccgatt tctgccgttc ttcacaattt
      601 tcatatctta accataatac agatcctcgt aaaacactac ttatatattg ccgatcattg
      661 cccagatgtt tcgatttttt ctatattttt tctctcttct gttgtataat accgtttatc
      721 agatacggag gattcttttg ccatgaaaaa cgattcgact tcttttttcg aaaacaatca
      781 ttttgctaac agctatgttt acgatatctc tatttcgcat gtccccgcct atttcggtct
      841 gcactggcat cactatattg aaattgtgct tgcacaggat gacggcatta tctatgaagt
      901 aaatggagaa acctttccgc taaagcccgg tgatcttctt tttatctggc ccggcgagct
      961 gcacgctgtt tccacaacca acagagaaca tcgcctgatc atgcttcagc tcgatgcaga
     1021 ttttctggaa aaacgcgtgg atttccagtc tgttgcttat cttttttatc agatccgtct
     1081 tctgaatcag aacaacttcc cggaaatcga aaagcttcgc ggatttctga cagagctgcg
     1141 caggctctcc ctctccggta tcagtttttc cgagctgcgc ggatgcgttc tgctgtatca
     1201 gttttttatt ctgctcggcg aaaatctctc cgtgccgacc gaaactgccg caccgcaaaa
     1261 aacaggccat tccgcacagg tacaccgcca gatggttgct gcatgtacct ggatctcaag
     1321 tcactgcacc gaaccggttt ccctggaaga agcatcaaag gtcgccggat tctcccgctg
     1381 ccatttttcc aagctgttcc gcgcatacac cggcctctcc ttcacggatt tcgtcaccag
     1441 ggaacggctc catatcgccg aaaacctgtt aaaaaaaccg gatctcacga tcaccgaagt
     1501 gtcgctcgaa tccggtttta acagtatttc tacgtttaac agagtctttt tgaaatcaaa
     1561 aaatgtaagc cctaccgact tccgtcagat gtacctgcaa tcctatcaga aataggctat
     1621 gaattcggca tggcctccgg tctttcgcag gtggatggaa ccttctcaaa tgcacccgtt
     1681 tccgcactct tcataatctg atcgatcgta tcgagtacgt gatatgccat tttggcattc
     1741 gcacggttta gtctgccctc tgcaattgct tctgccatct cggacggacc gaggccgcgg
     1801 ctgttttcgc tgtaggcaaa gccataatcc agcacttccg gaactgcctg ctgtgtccag
     1861 tccttcacgc ccggaatgta gacgatatcg ccgccgaaat tgttcgggtc caccagcttt
     1921 aaaatacctt ttttgccgta aattgtgaat accgcctgat cgttgatggt gctgtcgcca
     1981 ttgacacaga atgtgccggt cacgccgttt ttcatttcca gaatcgtgaa agcctggctc
     2041 tcgttgctgt agtcaaattc ctggccgtat tccgggctgt ccgggacgac attgacgcgc
     2101 ttcggcgcaa ggttgcgcac ggaggaaacg acacggtcca ccgggccgaa aaggctgacg
     2161 atggcggtca gataataaac accgaaatcg tagcagatac cgccgcccgg catccgcaga
     2221 aaacggaaga tgctcgccag caaatcaaga tcgcggtttg cgcatgcttc gaaagatgtc
     2281 acctcgccga tcagcccgtc gtcgatcgct tttcttgcgg tctgcagtgc cgatccaagg
     2341 aaggtgtccg gtgcggaacc gagatagaga tgtttctcct ctgccagctc gatcagtttc
     2401 gctgccgttt ccggtgcgag ggtcattgtt ttttcagtgt aaacgtgttt tccggcttcc
     2461 agtgcctgac ggatcagccc ctcgtgggtc ggtgccggtg tcagaatgac taccatttca
     2521 atcgacggat ctgccagaat ttcctcgtag gtacagccct tgatgccata ctgctctgct
     2581 ttctttttcg catgttcaag atggcctgcg cagcatgctg ctacctcaag attctcaaag
     2641 ttatgaatca tgttcgtcag ataaatatca ctgatcgctc cgcagccgac aacgccgact
     2701 ctcatttttt tcgtttctgt tgttcccatt tgtaaaaatc ccctttactc tttgtttttt
     2761 gattctgttc taataaaaac ccttattttt tttatacaac gaaatgtctg aaaaaacaag
     2821 cattccggct catatttcac tttcaaaatg aaaaaaccgc ccacggtttc tgcagattct
     2881 ctgcatcacc gcggacggcc tttttctgtt gtttactcca cgcttttcag cgattcaatc
     2941 atatcaattt ttcttaaccg gtagaacatc ataaaattga cgaacagagc aaacgccagg
     3001 gtaatcaggg cgctccagcc gtagctccgc gggaacacgt ttcgtccgaa catcatcata
     3061 tcgacctcga cggtctgaat tacgcaatga tgtaaaaacg ttccaattcc catgcccgcg
     3121 ataacgccga acagtgtcag caccatgttt tcccggtaca cgtacgatgc cacctcgccg
     3181 tcgaagaagc cgagcacttt cagcgttgcc agctctctct gtcgttcggt gatgttgatg
     3241 ttgttcaggt tgtacagcac cacaaacgcc agcaggcccg ccgatacgat cagcaccacg
     3301 atgacgatgt cgagggaacg aagcatgttg tcaatctgat caatcaggtc ggatgtgaac
     3361 gagattgccg ctacaccgtc gtacgtcatg attttttctc cgagtgctgt ctcataattg
     3421 cccgacgtgt cctgatattt catcagaagc tggttgtagt ccggcgctcc gccgaacagc
     3481 tcctcgtaga gatccggggc cagatacagg taatggcgca cgtagttttc gacgatgatc
     3541 gaaatcttta cgtccacaat atcgtcgcct tttttcagct gaaccgtgtc gcctgctttt
     3601 acgccgagca tcgttgctgt cttttcgctg atggctgcgc cgccgtcggt cgggaattcg
     3661 taaacttctc cgcttttccg gtctcggaag gcatagaagt cattgaaatt cgtcagttct
     3721 ttcggcactt caagcactgc atccacggtc tttttgtcgg tctgcagttc gatgttctgg
     3781 cagtacattt ccagcagctc atcgatgccg ctgtagtttt ccagatcggt ctgcaatgct
     3841 tctttttcgg attcatccac atctgaattc agcacagcat tcgcctgata ggtgaagagg
     3901 ctgacgaact ggtttttcgc aatcgcggtg atggaatcct gaaggccgaa accgacgagg
     3961 atcagaccca tgcatccgcc gattccgata acggtcatga agaatcgttt tttataccgc
     4021 accagattcc gcacggttga tttctgcgta aagttcagat gtttccagag tacgccgatg
     4081 cgttccagga aaatccgttt gccgtttttc ggtgcttccg gccgcatcag ctctgccgga
     4141 cctgcggcga gttctttgta gcatgccgcg atcgtcgcca cgccggtgca ggctgcactc
     4201 gccagaatcg caaggcctcc ctgctcccag ttcagcgggg tcatgtaata cgggagtccg
     4261 gtatacagca tgccgtaggc attcataata acccagggca ggattttttc tccaaccacc
     4321 acgcccgcga gagcgccgga tacggcggag agcatcgcgt atgcgaaata tttaaaggcg
     4381 atcacgccgt cggaatagcc gagggctttt aacgttccga tctgctgacg ctgttcctcg
     4441 atcattcgtg tcatcgtcgt caggctgacg agagcagcca cgaggaagaa gataaccggg
     4501 aagaattttc cgatattcga gatacggccg gcatcctgac cgaagctctc agttgacgtt
     4561 accttattcc gtccccagac gtaccacgtt ggttttttca ggtctgcgag tgttttctca
     4621 ccgtccgcaa tttcctgctt cgcatccgcg atttctggtt cggcgtcggc ttttgctttt
     4681 tcatattcgg ctttgccgtc ctcaagttcc ttttctttat cggcaatttc ctgtttcgca
     4741 tccgcaaggg agatttttcc gtcggcgatt tcctgtttcg cgtcggcaag ctgggtttct
     4801 ccgtcggcaa tcgtctgctt ggcttcggcg agctgacttt ctccatcggc gatcgtctgc
     4861 tttgcttctg ctaactgggt ttcgccatcg gcgatcgtct gtttcgcgtc ggcgagctga
     4921 ctttctccac tggaaattgt ctgctttgca gcggcaagct gggcctcacc cgactgaatc
     4981 gtctgtcggt acgatgcaat ctgctgttcc ccatcagcaa gctgggctct ggcagccgca
     5041 agctgagttt ctccgtcttt taatttactg cggttcgtct caatctgagc ttctccgtcg
     5101 gcaatcgctg ctttggcgga tgcaagctgg gcttcgccat cctcaatctg tttctgataa
     5161 gcttctgcct gtgcttctcc atcctgaagc tgggcaagag cctctgtaat ctgagcttcg
     5221 ccgctttcca gttcttcttt tgcagaagca atctgtgcct cgcctgcggc gcattcctgc
     5281 tctttttctg ttaattgtgc atctgcagcg gcaagctgac tttctgcgtc ggtaatctga
     5341 gcttctgcag ccgcaagccg ggcttccgca tcagcgcttg ctgcttcaaa cgctgcgatg
     5401 ttttgttcca gaaatgtttt ctgctcctgc atcgtgaaaa gctgctgtgc taactctgtc
     5461 tgctttgctt ccagcgcagt tttctgttcc aacagggata catatgcttc atcttcctct
     5521 gtcagtccgt caagctgagc ctcaatcacc tcgatctgac cagtcacttc tgtctgcgca
     5581 gcctccacca tcggaagctg cctggaaatc tgggctaact gatccttggc tgtctcatac
     5641 tgactcttct gttcttcata cgctgccttc tgcgcttcat actgtgtgcg tcctgcagcg
     5701 gtttctgctt tcttgttgtc cagttcctcg cgtgcagcgg aaagctgctc ctttgcaata
     5761 ttcaattccg tttctttttc ggaaagctgc tcttttgcag tattcagctc cgtctgcttc
     5821 cgagtcagtt cggtttttgc cgccgaaagc tgcgtcattt tttcctctaa ctcttttttg
     5881 ccggatgcca gttctgtttc tttctctgca agctgtgctt ttccgtcgga aagttctttc
     5941 gcttttgcct caagtgtggt ttttccactt gcaagttcat cggctttctg attcagctcc
     6001 gcttttccgc tttccagctc tgccgccttt gcttccagcg tcgcttttcc gctcgcaagt
     6061 tccgcctctt tatctttcag cgttgccttg ccggatgcca gttcctgctc tttatcggcg
     6121 atggttgctt tcgcactgag aagttcctgc tctttgtctg caatggtctg ttttccgtca
     6181 aggagttcct gttctttctc agcaatcgtc tgctttccgt caagaagctc ctgttctttg
     6241 tcctttaacg tctgttcgcc gtcgagaagt tcctgctctt tgtccgcaat cgtcactttc
     6301 gcgtcggcaa gctgctgtgc accgtcatcc agctctgttt ttgcgtccgc taatttctgc
     6361 tctgcatcag cgacctgctg ttttgcatca tcaatctttt cctgtccttc ggttttgact
     6421 gccgtatacc gccggtcgca cgccgcatcc tcgagcgctt caatcttcgt ctgtacagcc
     6481 ttcaccgtct catcatacgc atcgctgtag ctcgcctctt cctgtgcgcc gtccgcctgg
     6541 acgtaaatct ctgtatagag atccgaggta aatgtctccg gaagcagcag tgcaaaactg
     6601 ttgatgcttc cgtttccaat cgagcccgtt ccccggttca gatccatata ataagggagt
     6661 gtcgccgaac caacaatcgt aaacgtgtcc ccggtcagcg aatctgagag cggatcctcc
     6721 gttccggaag tgaacgttac ctgatctccg atctcacagc cggaactttt tagaaactgc
     6781 gtatcaacaa gaatttcatc ctctttctcc ggcatccgtc cctcgaccac gcgcggctca
     6841 ttcacaccgt ccgtcagcgc aatcagcttc acaacctgct cgctctcgcc gatgtcatgc
     6901 agcacttcca ccgtttttcc gccgtagacc gctttcacgc catccgtctt cgcgatatcc
     6961 gccagatcat cgtctgtcag ccccagcgtt ccaagaactt tcaaatccat ataatttact
     7021 tcatcataat accggtccgc cgaaaccttc atatctcctt ccgccgaccg aatgcccgag
     7081 aaaaacgccg cgccaagaag cacgatgaaa aagactgaca gaaaccgccc cttgttcttt
     7141 gtgatttccc ggataaagtc ttttcgaaat gctctcatcg tccacctacc attctatctc
     7201 ttccaccggc gtcgggttct catttgtttt cattcccgcc acttttccgt ttcggatttt
     7261 aatcacgcgg tccgccatcg gtgtcagcgc cgagttgtga gtgatgacaa tgaccgtcat
     7321 ccccttctcc cggcaggtat cctgcagcag ctttaagatc gcttttcccg tcacataatc
     7381 gagcgcgccg gtcggctcat cgcagagcag cagtttcgga ttcttcgcca gcgcacgcgc
     7441 aatcgccaca cgctgctgct ctccgcccga aagctgcgcc gggaagttat tcattctctc
     7501 ttccagccca acctccgcaa gcacctccga cgcatccagc ggatgcttac aaatctgcat
     7561 cgccagctcc acattctcga gcgccgtcag gttctgcatc agattataaa actgaaaaac
     7621 aaatccaata tcctcccgcc ggtactggat cagcttcttg ccgcggtatt tgctgatttc
     7681 cgcgccgtcc accagcacct ttcctgaagt cgctgtgtcc attccgccta aaatattcag
     7741 caccgtcgtc ttacccgcac cgctcggtcc aacaacaatc acaaactcac ccttttccac
     7801 atcaaa
//
LOCUS       DAOMUF010000099         7767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2452_length_7767_cov_4.397044, whole genome shotgun sequence.
ACCESSION   DAOMUF010000099 DAOMUF010000000
VERSION     DAOMUF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2452_length_7767_cov_4.397044"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1135)
                     /locus_tag="PLN77_06755"
     CDS             complement(104..1135)
                     /locus_tag="PLN77_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRM44011.1"
                     /translation="MKKMTKKKKLFRKVTALAAVIALGASLAGCGSSGEETTQRYSWP
                     LATASPEDTVTQIFAEKFAEEISELSDGRMKIQVYANSTLGGDRDLLETCADGDIPFV
                     VQNTAPQVSFMGDLAVFDLPCVFDSLDECREKIDNPEFYELISNVYTEGGYHLLGMAD
                     QGFRVMSTNKPVYSFSDFKGQKIRTMENSYHLAFWKAIGANPTPMSFSEVYIGLQQHT
                     IDAQENPYEVIVSNNLYEQQDYVVETNHLPHLISLIVNDDFFQDLPADEQEIMTEAAK
                     IATEYAREQSDARIVDKIAKIEESGTKILTLDDQTRADIREASQSVYESIKENIDPAI
                     YNAYMDGIE"
     gene            complement(1152..2441)
                     /locus_tag="PLN77_06760"
     CDS             complement(1152..2441)
                     /locus_tag="PLN77_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRM44012.1"
                     /translation="MPVVVLFLIFVALLVIAVPVSITLGITSVLPSVFDPSFTVGAKY
                     LIRAMFGGLDSFPLLAVPMFVLSGIIMAKGGISKKLFDVFAYFLGKFTAGMPCAVIVT
                     CLFYGAISGSAPATVAAVGSMTIPILTTLGYDLTFSTAIVAVAGGLGVIIPPSIPFIM
                     YGMASGASVSDLFLAGIVPGLMIGLLLMVYAVYHCKRYGEDKEKIHAEVKVLHDKGLL
                     RVLKESFFALLSPVIILGCIYSGVASPTEAAVISVFYALIISLFVYRSIRIRDIWSIL
                     VEAIRTFTPILFILAASTAFSRVLTLMQVPQTVSEFILNNFHSPVAILLIINVFLLIV
                     GMVMDTTPAILILTPILLPIIQAIGMDPIQFGVIMVVNLAIGFVTPPIGVNLFVASSL
                     TEVPVMQIAKKAMPMIGFFLAALLLITFIPAISIGIL"
     gene            complement(2441..3073)
                     /locus_tag="PLN77_06765"
     CDS             complement(2441..3073)
                     /locus_tag="PLN77_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRM44013.1"
                     /translation="MKRIFHWLDENLEEFLLVIGLIAMTLIMGVQVFCRYVLGMSLSW
                     SEELTRYIFIWCGFLSVSYCSKKCLSIKIEQFVAIFPRRGKAIFKIVNHTFELIFFIY
                     MIPFAFSYMMSSVKSGQLSPACKIPMYFIQAAPLVSFVLVAFRVLQRWMIEFRVARGE
                     NVFDPAHPERNTPESFIEANAGADNATENALNAGIDNRIHTIKNSNEEER"
     gene            complement(3178..3939)
                     /gene="aroD"
                     /locus_tag="PLN77_06770"
     CDS             complement(3178..3939)
                     /gene="aroD"
                     /locus_tag="PLN77_06770"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRM44014.1"
                     /translation="MNTVKVRDIEIGAGAPKIIVPIVGVTKNDIIEEAKTFHSIPVDV
                     VEWRVDWFEGVFDFAQVEDVLKDLREALGNIPLLMTFRTSKEGGEKAIEPAAYAELNI
                     KAAQTGYVDLVDVEIFTGDDIVKKIIDGAHAAGVKVIASNHDFFKTPAKADIIYRLRK
                     MQDMNADIPKIAVMPQNRKDVLTLLAATEEMVTNYADRPIITMSMAGTGVISRLCGEV
                     FGSSMTFGAAKKASAPGQMGVNDLSTVLDLLHKAM"
     gene            complement(4048..5262)
                     /locus_tag="PLN77_06775"
     CDS             complement(4048..5262)
                     /locus_tag="PLN77_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM44015.1"
                     /translation="MNKKYLPSALILYLNYFIHGVGCSILGQAVIKEALAAAWGVEAM
                     AITAISAALGLGRLIALPFAGPLSDKLGRRISTAIGSASYAIYLIGLALAFNAGTHGG
                     YTIAYVCAIIGGIANSFLDTGIYPAVSEIIYKAPGVATMGIKFFIAIAQMLLPFVLGV
                     AVTSTAAGFTSYNPLFYGCGIAYIVLFVLVFLFPLPDADQKASGKKEGLIDSLKHTHF
                     SFESIAMILIGFTCTGTFQLWLNCAQNFAKENVGWANPSIMQTYYSAGTMLALVVTAF
                     LTRKIKDVRFIVVYPAICLVTMIAVLMGQSKPLMIAGAFIIGWAGAGGLLQIATSVCN
                     MLFPKIKGTVTALVMIASSLCNYTILTAASKMQPSGVMTMNIVLTAVGVALGLFVNIR
                     YKKMVELAQQDN"
     gene            5542..6489
                     /locus_tag="PLN77_06780"
     CDS             5542..6489
                     /locus_tag="PLN77_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM44016.1"
                     /translation="MTLNQILYFRKVARLENYHQAAEELYISQPSLSRSMAALESELG
                     ITLFEKKGRGVVLTNAGRLFLEHADRIAGDCDIAAGKMKELASGGGKINIGYIFPLAG
                     HYIPHNVRTFLDKEENKNVAFSFWQNHTPAIAQKVKSGELDLGFGGFLKRDDMEFFPL
                     IQQPLVIVSPEQHPIADEPEVPLVKLTRYPVIGYDRASWMGAYTGHLYRKYQLHPNII
                     MECPDEYSIVALVRENFGIALMPRTDILEQADGIRIHTLKGLEIYHQTFMFWMKNRYR
                     LPAVERFTEYMKQHADIIEESRNDSENVSKVYLKDIVNF"
     gene            6561..>7767
                     /locus_tag="PLN77_06785"
     CDS             6561..>7767
                     /locus_tag="PLN77_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRM44017.1"
                     /translation="MKSAYPKIFEPITIKRTTIKNRIAMTPMGTNYGETSGEMSNRHM
                     NYYSLRAKGGVGLIILENANIEYPVGSNGTSQIRIDHDSFMPRYYQLVENLHKNGATV
                     AIQINHAGASASSARTGMETVSSSNIPTKAGGETPRPMTKEEIMTTVKKYGEAAKRVQ
                     AIGFDAIEIHCGHSYLMSQFISPYYNKRTDEFGGSVENRLRFPRMVLEEVRKNVGPWF
                     PIIVRISAEERVPGGNTLEDTLEYLEYLDEFVDMYDVSCGLNPSLQYQIDSNFLPDGW
                     RSYMARAVKDKFKKPVINAGNYRDPKVVEKVLESGDVDIVGMGRGLIAEPNWVKKVEN
                     GEEDLLRKCISCNVGCAGNRIGVNRPIRCTVNPAVPEGDVYKALKVNKNCNVVVVGAG
                     TAGMEAACTA"
BASE COUNT         2098 a         1938 c         1850 g         1881 t
ORIGIN      
        1 agtcttacag tttcgaccgt gcaaggcaaa ttttgtgtaa aaaaatccgg ccgcgaaaca
       61 ctgtttttca gtgcccctca gccgggaatt tcattttctt ttcttactca attccatcca
      121 tatacgcatt gtaaatcgcc ggatcaatat tctctttgat cgactcatac acgctctggg
      181 atgcctcgcg gatgtcagct cttgtctggt catcgagtgt cagaatcttc gttccgctct
      241 cctcgatttt cgcgatcttg tcgacaatac gggcatcgga ctgctctctt gcgtactcgg
      301 tcgcaatctt tgcggcctct gtcatgatct cctgctcgtc tgcaggcaga tcctggaaga
      361 aatcatcgtt gacgatcagg gaaatcagat gcggcagatg gttcgtctcc actacgtaat
      421 cctgctgctc atacaggtta ttcgaaacga tgacctcata cgggttctcc tgcgcatcaa
      481 tcgtgtgctg ctgcaggccg atgtaaacct cactgaagct catcggcgtc gggttggcac
      541 cgatggcttt ccagaatgcc agatggtagc tgttctccat ggtacggatc ttctggccct
      601 tgaaatccga aaagctgtaa accggcttat tcgtactcat gacacgaaat ccctgatctg
      661 ccataccgag caggtgatag cctccctccg tataaacatt gctgatcagc tcgtaaaact
      721 ccggattatc gatcttctca cggcattcat cgagagaatc aaacacacac ggcaaatcaa
      781 acacggcaag atcacccata aaggaaacct gcggtgccgt attctgtacg acaaacggaa
      841 tatcgccgtc agcacacgtt tccagcagat cgcggtcgcc gccaagcgtt gagtttgcgt
      901 aaacctgaat tttcatcctt ccatcgctca gctctgaaat ctcttccgcg aacttctcgg
      961 caaagatctg tgttaccgtg tcctccgggc tcgccgttgc cagcggccag gaatagcgct
     1021 gtgtcgtctc ttctccggag gagccgcagc cggcaagcga tgcgccaagc gcaatgacag
     1081 cggcaagtgc cgtcactttt cggaaaagct ttttcttttt tgtcatcttt ttcattgtcc
     1141 ttcgctcctc cctataaaat tccaatcgaa atcgccggaa taaacgtaat cagcagcagt
     1201 gccgcaagga agaaaccgat catcggcata gccttctttg cgatctgcat gaccggaacc
     1261 tcggtcaggg agcttgccac gaacaggttg acgccgatcg gcggtgttac gaagccgatc
     1321 gccaggttga caaccatgat cacaccaaac tgaatcggat ccataccgat tgcctgtatg
     1381 atcggcagca ggatcggggt cagaatcagg attgccggtg tggtatccat caccattccg
     1441 acaatcagca ggaatacgtt gatgatcaga aggatcgcaa ccgggctgtg gaagttgttc
     1501 agaatgaact cactgaccgt ctgcggcacc tgcatcagcg tcaggacacg ggaaaatgct
     1561 gtcgatgccg ccaggatgaa caggatcgga gtaaaggtgc gaatcgcctc aaccagaatg
     1621 gaccagatgt cgcggatgcg gatactcctg tacacaaaaa ggctgatgat caatgcatag
     1681 aatacggaga taacagccgc ctcagtcggg gaagcgacac cgctgtagat acagccaaga
     1741 atgatcaccg ggctgagcag tgcaaaaaag ctctctttta aaacgcgaag caggcctttg
     1801 tcatgcagca ctttgacttc tgcatggatc ttctctttgt cctccccata ccgtttgcag
     1861 tgataaacgg cataaaccat cagcagcaga ccgatcatca gacccggtac aattccggca
     1921 aggaacaggt cactgaccga agcgccggaa gccataccgt acatgatgaa cggaatactc
     1981 gggggaatga taacgccaag gccgccggca actgccacaa tcgctgtgga gaatgtcaga
     2041 tcataaccaa gagtagtcag aatcggaatt gtcatgcttc cgacagcagc aacggtcgcc
     2101 ggagcggaac cggaaatcgc accatagaaa aggcaggtga cgataacggc gcacggcata
     2161 cctgcggtga attttccgag aaaataggca aagacatcaa acagtttttt ggagatgcct
     2221 cccttagcca tgatgatacc ggagaggacg aacatcggaa ctgccagcag ggggaaggaa
     2281 tcgagacctc cgaacatggc acggatcaga tatttggctc ctaccgtaaa ggacgggtca
     2341 aacaccgacg gaagcacaga tgtgattcca agggtaatcg ataccggaac agcgatgacc
     2401 agcaatgcaa caaaaatcaa aaataatact acgaccggca ttagcgttcc tcctcgtttg
     2461 aatttttaat cgtatgaatg cggttgtcga tacccgcgtt cagggcattt tctgtggcgt
     2521 tatctgctcc ggcatttgcc tcaatgaagg attccggcgt attgcgctcc ggatgggccg
     2581 ggtcgaaaac gttctcaccg cgtgccacgc ggaactcgat catccaccgc tgcaaaacac
     2641 gaaaggcaac cagcacaaag gagacgagcg gtgctgcctg gataaagtac atcgggattt
     2701 tgcaggccgg ggaaagctgg ccgcttttaa ccgacgacat catataggaa aaagcaaacg
     2761 gaatcatata gatgaaaaaa atcagttcaa atgtatgatt cacaattttg aagatggctt
     2821 ttcctctccg cgggaaaatc gcaacaaact gctcgatctt gatggaaagg catttcttac
     2881 tgcagtagct gacgcttaaa aaaccgcacc agataaaaat atatctggtc agctcttccg
     2941 accacgacag cgacatgcca agaacgtaac gacagaaaac ctggactccc atgatcagcg
     3001 tcatggcgat cagaccgatc acaagaagaa attcttcgag attttcatcc agccagtgaa
     3061 atattctctt cataaaaggc atacctccgt taaatttata acagaacaat aatacaaaag
     3121 ggacatcggc atcaacagca aagctaaaaa taccggtgtc ccctaaagaa aaaacggtta
     3181 catggctttg tgaagcagat ccagaacggt gctcagatca ttcacaccca tctgacccgg
     3241 tgcggatgct ttctttgccg cgccgaaggt catggaagaa ccgaatacct caccgcagag
     3301 acggctgatt acaccggtgc ctgccataga catggtgata atcggtctgt ccgcataatt
     3361 ggtgaccatt tcctctgttg ccgcaagcag ggtcagaacg tctttcctgt tctgcggcat
     3421 aacagcaatc ttcggaatat ctgcgttcat atcctgcatt ttgcgcagtc tgtagatgat
     3481 gtctgctttt gcaggagttt tgaagaaatc atgattggaa gcgataacct ttacacctgc
     3541 tgcgtgtgca ccgtcgatga tcttctttac aatgtcatct ccggtaaaga tctcaacatc
     3601 caccagatcc acatatccgg tctgtgctgc tttgatgttc agctccgcat aggctgccgg
     3661 ctcaatcgct ttttcaccgc cctctttgga ggtacggaat gtcatcagaa gcggaatatt
     3721 gccgagtgct tcgcggaggt cttttaacac gtcctctacc tgcgcaaagt cgaatacgcc
     3781 ttcaaaccag tctacacgcc attctacaac atcaaccgga atcgaatgga aggtttttgc
     3841 ttcttcgatg atatcgtttt tggtaacgcc tacgatcgga acgatgatct tcggtgcacc
     3901 ggcaccgatt tcaatatcac gaacttttac tgtattcatg tgtttttcct tctttcttca
     3961 atttaaccct gacttttctt tgcaggcttt tctttttcac gtccggattc cttggtactt
     4021 cgtacttccg caatccttaa cctcatttta attgtcctgc tgtgcaagtt caaccatttt
     4081 tttatagcgg atatttacaa acagtccaag tgcaacacca actgcggtca gaacgatatt
     4141 catggtcata acgccggacg gctgcatttt agatgctgcg gtcaggatgg tgtagttgca
     4201 gagagaagat gcgatcataa ccagtgcggt tacggttcct ttgattttcg ggaacagcat
     4261 gttgcaaacg gaagttgcga tctgaagcag tccgcctgcg cctgcccatc cgataatgaa
     4321 agcaccggca atcatcagcg gtttgctctg tcccatcagg acagcgatca ttgttacaag
     4381 gcagatagcc gggtaaacaa cgatgaaacg aacgtctttg atttttctgg tcaggaatgc
     4441 ggttacaacg agtgccagca tagttcctgc agaatagtag gtctgcatga tggacggatt
     4501 cgcccagcct acattttctt ttgcaaagtt ctgcgcacag ttcagccaca gctggaaggt
     4561 accggtgcag gtgaatccga tcaggatcat ggcgatggac tcgaaagaga aatgggtatg
     4621 cttcaggctg tcgatcagac cctctttttt gccggatgct ttctggtcag catctggcag
     4681 cgggaacagg aataccagta caaaaagtac gatatacgca attccgcatc catagaataa
     4741 cggattgtag gaggtaaagc cggctgcggt agaagtaacc gctacaccaa gaacaaatgg
     4801 aagcagcatc tgtgcgattg cgatgaagaa tttgattccc atagtagcaa cgcccggagc
     4861 tttgtaaata atttcggata ctgccgggta aataccggta tccagaaagg agtttgcgat
     4921 accgccgata atggcgcata cgtaagcgat ggtataacca ccgtgtgttc ctgcattaaa
     4981 tgcaagggca aggccgatca gataaattgc ataagaagca cttccgattg cggtggaaat
     5041 gcgacgtccg agcttgtcgg acagaggacc tgcaaatggc agtgcgatca gacggccaag
     5101 accgagtgct gcggaaattg cggtaattgc cattgcttcc actccccatg ctgctgccag
     5161 agcctctttg atgactgcct ggcccagaat agagcatcca acaccgtgga taaagtagtt
     5221 caagtacaaa attaatgcac ttggcaggta tttcttgttc atggtttctc tcctttttaa
     5281 atagattatg ttgaggtcaa attgtctttt tcctctgttc ggttcatcct tttgttttgt
     5341 cacaaacaac agattaccag tttcgtgaat gttaaaaaaa taacatttgc ctttatttta
     5401 acagtgaaaa cgtgtacggt ctaatgcttt taaaggaaaa aaccaatgca ttgaatgcat
     5461 tgatgtggta tacttgagtt atcataataa gaggaagcaa agactttctt acggaaccat
     5521 tttttcagga ggaaaaacca catgacactg aatcagattc tctatttccg aaaggtcgcc
     5581 cggctggaaa attatcacca ggcggcagag gagctttata tttcccagcc atccctgagc
     5641 cgttcgatgg ccgcccttga aagtgagctt ggaatcacgc tgtttgaaaa gaaagggcgc
     5701 ggtgttgtcc ttaccaatgc cggccgtctt ttcctggagc atgcagaccg gatcgcaggc
     5761 gactgtgaca ttgcggcagg caagatgaaa gaactcgcct ccggtggcgg aaaaatcaac
     5821 atcggttata tttttccact tgccggacat tatattccgc acaatgtccg cacctttctg
     5881 gacaaagaag aaaacaaaaa cgttgccttt agcttctggc agaaccatac cccggccatt
     5941 gcacagaagg tgaaatccgg cgaactcgat cttgggtttg gcggcttttt aaagcgcgac
     6001 gacatggagt tttttccgtt gatccagcag ccgcttgtca tcgtgtcccc ggaacagcat
     6061 ccgattgccg atgaaccgga ggttccgctt gtaaagctta cccgttatcc ggtgatcggc
     6121 tacgaccgcg catcctggat gggggcctac accggtcatc tctaccgcaa atatcagctt
     6181 catccgaata ttatcatgga atgcccggat gaatattcga ttgtggcact ggtacgcgaa
     6241 aacttcggca tcgcgctcat gccgcgaacc gatattctgg aacaggcaga cggtatccgc
     6301 atccacacac tgaaaggtct cgagatttat catcagactt ttatgttctg gatgaagaac
     6361 cgctaccggc ttccggctgt cgaacggttt accgaataca tgaaacagca cgctgacatt
     6421 atcgaagagt ccagaaacga tagcgaaaac gtatcaaaag tttatctgaa agatattgtt
     6481 aatttttagt cgaaagagtt atgatggatt tgtcacaaac agaggattac cgctcatgta
     6541 atgaataagg agaaattttc atgaagagtg cttacccaaa aattttcgag ccaattacca
     6601 tcaaacgaac aacgattaaa aaccgtattg caatgacccc gatgggaacc aactacggtg
     6661 agacaagcgg tgagatgagt aaccgtcata tgaattatta ctctctccgc gcaaaaggcg
     6721 gcgttggtct tattattctg gagaacgcca acatcgagta cccggtcggt tccaacggaa
     6781 ccagccagat ccgtatcgac cacgacagct ttatgccgcg ctactatcag ttagtcgaaa
     6841 accttcataa aaacggtgct accgtagcga tccagatcaa ccacgccgga gcttccgcat
     6901 cttccgcaag aaccggtatg gaaaccgttt cttcttccaa catcccgaca aaagccggcg
     6961 gagaaacccc tcgcccgatg acaaaagaag aaatcatgac aaccgtgaaa aaatacggcg
     7021 aggctgcaaa acgtgtgcag gccatcggct ttgatgccat tgaaatccac tgcggccatt
     7081 cctacctgat gagccagttc atctccccgt attacaataa aagaaccgat gaattcggtg
     7141 gttctgtaga aaaccgcctg cgcttcccgc gtatggttct cgaagaagta cgtaaaaacg
     7201 tcggaccgtg gttcccgatc atcgttcgta tctctgctga ggagcgtgtt ccgggaggaa
     7261 atactctgga agataccctc gagtacctcg agtacctgga tgagttcgta gatatgtatg
     7321 acgtatcctg cggcctcaac ccatccctgc agtatcagat cgactccaac ttcctgccgg
     7381 atggatggcg ttcctacatg gcaagagccg taaaagataa attcaaaaaa cctgtgatca
     7441 acgctggtaa ctaccgcgat ccgaaggtcg ttgaaaaagt tctcgagagc ggcgatgtag
     7501 atatcgtcgg aatgggacgc ggcctgatcg ctgagccgaa ctgggtgaaa aaagttgaga
     7561 acggcgagga agatcttctt cgaaaatgta tctcctgtaa cgttggatgt gccggaaacc
     7621 gtatcggcgt caaccgcccg atccgctgta ccgtaaaccc ggctgttccg gaaggcgatg
     7681 tttacaaagc actgaaagtg aacaaaaact gtaacgttgt tgttgtcggc gccggtactg
     7741 ccggtatgga agcagcctgc accgcag
//
LOCUS       DAOMUF010000100         7573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2547_length_7573_cov_4.138069, whole genome shotgun sequence.
ACCESSION   DAOMUF010000100 DAOMUF010000000
VERSION     DAOMUF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2547_length_7573_cov_4.138069"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    195..204
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(404..2242)
                     /locus_tag="PLN77_06790"
     CDS             complement(404..2242)
                     /locus_tag="PLN77_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44018.1"
                     /translation="MRVGNVKEIVFSKDPKQMNWLREEFPYAEVKCPPEFSAEVQNEK
                     DGDVLTTKIVVSYNGAHPYFTNAGSIGVSFPLQDRYTDSVTCRDYRCHAHVFCGENTS
                     YIMALRMGGAAPHLGMVLTKGSLSAYSIERDLKLQSNDRGCFWLHPSAQEFAPGDTMK
                     LEWKVFPHRGREDFREKLRAFPRVILVDAEQYVIYPGETSKVTIEPTFPAEKVTINGA
                     SLEKTENGVYEYLFENEKTGEYVLSICADEVKTTCRLLVQERPETLAAKRCAFIVDHQ
                     QYHGKIKELQGAYLPYDNEEKILVCTPENDFNAGRERTGMGVLIARALQQNLLKDREK
                     AEQSLREYHAFYLRELVNAATGLVCNCSGKDNSYFRLYNYPWAVTFFLECWKLWGEKE
                     DLKTAVRITEKFYEQDGFRFYPIEMPIVMLCHELEKAGEQEDLKTVKDLFRCHADQLI
                     EIGTAYPASEVNYEQSIVQPAAEVILQVYEVTGEEKYLRGAEQQIAVLELFDGQQPDY
                     HLHETAIRHWDGYWFGKRRVFGDTFPHYWSAENGRTFKRYARLTGNEEYNIRGEHSLR
                     GVLSMFFEDGTATCAYVYPYSINGQKTDFADPYANDQDWGLCMNLE"
     gene            2594..3790
                     /locus_tag="PLN77_06795"
     CDS             2594..3790
                     /locus_tag="PLN77_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRM44019.1"
                     /translation="MDTYVILKDLAIIIVFAKLFGILARKCKAPQVVGELIAGIVLGP
                     SVLGLVQQSDFLAQMAEIGVILLMFSAGLGTSLKDLLRTGVKSLLIACAGVFVPLVLG
                     AILYMSFYGFSAVGTEEFYHAVFIGVILTATSVSITVQALKELGKLKTEVGTTILNAA
                     IIDDVIGIIVLTVVIGFKDPTSNPLKVAASTILFFALAIVLGFLCFKLFNRMNSVFPH
                     TRRLPILGLALCFGLSYISEKYFGIADITGAYVAGVILCSLDSSDYIEEKMDISSYMI
                     FGPIFFASIGLKTTLSGMNEKFVLFAICFVIVGLVAKIIGCGLMSKGCGFTWGDSLKI
                     GVGMMTRGEVCLIVAQKGLNAGFLSGDFFSAVILLILFSSIATPIMLKILYEKMPTAK
                     DSAKAS"
     gene            complement(3877..5865)
                     /locus_tag="PLN77_06800"
     CDS             complement(3877..5865)
                     /locus_tag="PLN77_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRM44020.1"
                     /translation="MIYYVNNSAPKNGNGTKEMPFKFINDAAKIAKAGDEVLVAPGIY
                     HEYVDPVNGGTEDARIVYKSEKPLGAKITGAETMNDWEHYKDNVWVCRVDNGVFGNYN
                     PYTTMVGGDWYFAPVVRHTGAVYLNDRQLYEAETLEECIKGEVYAPSWEPEWSVYKWY
                     TEQDKEKNQTVIYANFQGKNPTEEKVEINVRRNCFMPSKTGVNYITFSGFDVSKAATT
                     WAPPAAYQDGMIGPHWSKGWIIEDCEVSNSKCCGISLGKYYDPENDHYFTRKHVKSPT
                     QMERDAVCRGQYHGWTKENIGSHIIRRCHIHHCEQTGIVGRMGGVFSIIEDNHIHNIN
                     NMQQLGGAEISGIKMHAAIDVVMRRNHIHHCTMGIWCDWEAQGTRLTQNLLHDNCPPE
                     GTPKAEGAMMSQDIFIEVGHGPTLIDNNIMLSPVSVRMATDGIACVHNLMLGSLTAVG
                     GGTGDRYTPYHIRHRTEVAGFMTFLHGDDRFYNNIFIQNYPVEETETVEDMGFKMEDN
                     QEVGTHVFDEYPTYDEWISHFELDKPADMSKLEPYHNKCHLPVWVNGNAYFNGAKACV
                     NEKENLMDNENQVKVELVEKDGHYSIKTNVYEFLKDFRTGIINSDILGYAFEPEQRFE
                     DPDGSTIIFDQDYLGEHRGVAAMPGPFADGAEAEKILW"
     gene            complement(5991..>7573)
                     /locus_tag="PLN77_06805"
     CDS             complement(5991..>7573)
                     /locus_tag="PLN77_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRM44021.1"
                     /translation="SWDQAFTVYTWYVEQDEEKNETVFYVNFQGKNPNEETVEINVRE
                     NCFYPSKEGIGYITLSGFVVKQAATQWAPPTAYQEGMVGPHWSKGWIIEDCEISDSKC
                     SGISLGKYRQPNNDNKWLKWKFKDGTQTERDCICQAQREGWTKENIGSHIIRRCNIHD
                     CGQTGIVGHLGGVFSIIEDNHIHHINNKQNLAGAEIGGIKMHAAIDVIIRRNHFHHCT
                     RGLWLDWQAQGTRVTQNLFHDNTLPNEENANPEGMDGIGEDIFIEISHGPTLVDNNVL
                     LSDRAMKLATQGVAVVHNLIAGSFTAVGRGVNNGSEKLPSPRYTPYHVPHRTEINGFM
                     TVLHGDCRFYNNIFIQKPVRAGMEEIRKLTGDNEWDDGNLTAGTAPYSGYPTLEEYVA
                     RFEGYCGMGSGKSPDLYYEKLPVWLGGNVYFNGAKPAEQEQDAVVDTEHEITIGVKEE
                     NGKWKLETNVYDYLPQNACAVISTETLGMAFEPEQKYENPDGTPIVFRYDYFGNRQGI
                     HPLPGPFASKEAAEEALS"
BASE COUNT         1663 a         2053 c         1617 g         2230 t
ORIGIN      
        1 gctattcggt tatgagcaag tagcgaagcg gattgcgaat atccgctttg acaagaaaaa
       61 atagagtatg gattgtttcc ataccctttt gattttcaca aatcctttgt ctggttctgg
      121 ttttattaca tcatcattga ggaaaatcat atcattattc ttacaccatg ccggaatcca
      181 tcttctgcaa atccnnnnnn nnnncatggg gaaacgcctg cgaagcggca gaaatcatag
      241 attacataaa tagttccgcg ccggtttctt cttttaaaac tgccttggcc tgtaatatct
      301 tctgcaaatc atccgccaga ttccgtgctg caatctgaac cttcaaaaaa cctccaaact
      361 gaatcactct gtatgatttc agtttgaagg ttctggtcat tctttattcc aggttcatgc
      421 acaatcccca atcctggtca ttggcatacg gatcggcaaa atcggttttc tggccgttaa
      481 tggaatacgg atacacgtac gcacaggtcg ctgttccatc ctcaaagaac atggacagca
      541 cgccgcgcag ggaatgctca ccgcgaatat tatattcttc gtttccggtc aggcgagcgt
      601 agcgcttgaa ggttcgaccg ttttctgcac tccagtagtg cgggaaagta tctccgaaga
      661 ctctgcgttt tccgaaccag tagccgtccc agtggcggat tgcagtttca tgcagatggt
      721 agtccggctg ctggccgtcg aagagttcga ggacggcgat ctgctgctcg gctccgcgca
      781 ggtatttttc ttccccggtc acttcgtaaa cctgaagaat gacttcggcc gccggctgta
      841 cgatcgattg ttcgtaattt acttccgaag ccggataagc cgttccaatc tcaatcagtt
      901 gatcagcatg acatcgaaac agatctttta ctgttttcag atcctcctgt tctcctgctt
      961 tttccagctc atgacagagc atcacaatcg gcatttcaat cggataaaaa cggaatccat
     1021 cctgttcata aaatttctct gtgatacgaa ctgccgtctt cagatcttct ttttctcccc
     1081 acagcttcca gcattcaaga aagaatgtca ctgcccatgg gtagttgtaa agccggaaat
     1141 agctgttgtc ttttcctgag cagttgcaaa caagacctgt tgctgcattt accagctcgc
     1201 gaagatagaa tgcatggtac tcacgaaggc tttgctccgc tttttcccgg tcttttaaaa
     1261 gattctgctg cagtgcccgt gcaatcagca cgcccattcc cgtacgctct cttccggcat
     1321 tgaaatcatt ctccggcgtg cagacgagaa ttttttcttc gttatcatac ggcagatagg
     1381 cgccctgcag ttctttgatc tttccatgat actgctggtg atcaacaata aacgcacacc
     1441 gttttgcagc cagtgtttcc ggccgctcct gcacgagaag acggcaggtg gttttcacct
     1501 cgtcggcaca gatcgaaagt acatactccc ctgttttttc attttcaaac agatattcat
     1561 aaacaccatt ttccgtcttt tccagcgatg ctccgtttat cgtcactttt tccgctggaa
     1621 atgtcggctc aatggttacc ttactcgtct ctcccggata aatcacatac tgctcagcat
     1681 ccaccagaat cacccgggga aatgctctta atttctcccg gaaatcttcg cgtccccgat
     1741 gcggaaatac cttccactcc agcttcattg tatctcccgg cgcaaattcc tgtgcggacg
     1801 ggtgcagcca gaagcagcct ctgtcattgc tctgcagttt cagatcgcgc tcaatactgt
     1861 aagcagaaag actgcctttt gtgagtacca ttcccagatg cggagccgca ccacccatgc
     1921 ggagcgccat aatataactg gtattctccc cacagaaaac atgtgcatgg caccggtagt
     1981 cacggcaggt cacactgtcc gtgtagcggt cctgcaacgg aaatgaaacc ccaatacttc
     2041 cggcatttgt aaaatacgga tgcgcaccgt tgtaagaaac cacaatcttc gttgtcagca
     2101 catctccgtc tttctcattc tgcacctcag ccgaaaactc cggcgggcat tttacctcgg
     2161 cgtatgggaa ctcctcccgc agccagttca tctgttttgg atctttggaa aatacaattt
     2221 ctttcacgtt cccaactctc attttttatt tcccctttcg ttttttcttt gatcccgctt
     2281 tttgaactcc atgtaaaatt tatttgctgc tttgatcata ctatatctac atgctctttt
     2341 ctatatctct ttttccatta ccgttcactg ctcttaatat aaccctcaca atcctctttt
     2401 ccagttttgt tttacaaaag aacggttcca tacgcctgca tcatttttcc gttttacttt
     2461 attttttctt tcttgccgat agtatttttt ctcaattttt gctatactga aaatgtatgc
     2521 gtatcaaact ccggcattgc tctttcaagc tgccgacaca tacataaaat tcataaaagg
     2581 gggtatcttt ttcatggata cgtatgtcat tttaaaagac ctggcaatca ttatcgtatt
     2641 cgcaaaattg tttggtattc ttgccagaaa atgcaaggct ccgcaggttg tgggcgagct
     2701 gattgccggt attgtccttg gacccagtgt gctgggactc gttcagcaat ctgactttct
     2761 cgcacaaatg gcggagatcg gtgtcatttt gctgatgttc tccgcaggtc ttggcaccag
     2821 cctgaaggat ctgctccgca ccggagtgaa atccctgctg atcgcctgtg ccggtgtttt
     2881 tgttcctctc gttcttggag ccatcttata catgagcttt tatggcttct ctgctgtcgg
     2941 aacggaagaa ttttaccacg ctgtttttat cggtgttatt ctgactgcga catcggtcag
     3001 catcaccgta caggccttga aagaactcgg caagctgaaa acagaggttg gaacaaccat
     3061 cttaaatgca gccatcatcg acgatgtcat tggtatcatt gtgctgactg ttgtcatcgg
     3121 ttttaaagat ccgacatcca atccgctgaa agtagcagcc tccaccattc tgttttttgc
     3181 ccttgcgatt gtgctcggtt ttctctgctt taagctgttc aaccgcatga actccgtatt
     3241 tccgcacacc cgccgtctcc cgattctggg tcttgcgctc tgcttcggac tgtcctacat
     3301 ttccgaaaaa tatttcggca tcgccgatat taccggtgca tacgttgcgg gcgttatcct
     3361 gtgttccctc gattcttccg attacatcga agaaaaaatg gatatcagct cctatatgat
     3421 tttcggaccg attttctttg caagtatcgg actcaaaaca acgctcagcg gcatgaacga
     3481 gaaattcgta ctctttgcca tctgtttcgt catcgtcggc cttgtcgcaa aaattatcgg
     3541 ctgtggcctg atgtcaaagg gatgcggctt tacctggggc gacagcctga aaatcggtgt
     3601 cggtatgatg acccgcggtg aagtctgtct gatcgtcgcg caaaagggtc tgaatgcagg
     3661 ctttttatcc ggcgatttct tcagtgccgt gattctgctg attctcttct cgagtattgc
     3721 aacaccgatt atgctgaaaa tcttatacga gaaaatgccg acggcaaaag attctgcaaa
     3781 ggcatcctga ctttcctttt accaaaacag ggcgctgtgc catttcctgc acagcgccct
     3841 gtttttttcg tatcaaccgg taagttctga tttcttctac cacagaatct tctctgcctc
     3901 agctccatcc gcaaacggtc ccggcattgc tgccacgccg cggtgctcgc caagatagtc
     3961 ctgatcgaag ataatcgtgc ttccgtccgg atcttcgaat ctctgctccg gctcgaaggc
     4021 gtatcctaaa atatcactgt tgataattcc cgtccggaaa tccttcagga actcatacac
     4081 gttcgtcttg atggaatagt ggccgtcttt ttccacaagt tccaccttaa cctggttctc
     4141 gttatccata agattctctt tttcattcac acacgccttt gctccgttaa agtaagcgtt
     4201 tccgtttacc caaaccggca gatggcactt gttgtgatac ggctccagct tgctcatgtc
     4261 ggccggtttg tccagctcaa agtggctgat ccactcgtca taggtcggat actcgtcgaa
     4321 cacatgcgtt ccgacctcct ggttatcctc catcttgaag cccatgtcct caaccgtttc
     4381 tgtttcctca accgggtaat tctggataaa gatgttgttg tagaaccggt catcgccatg
     4441 caggaatgtc atgaatccgg caacctcggt acggtgacgg atgtgatacg gcgtgtaacg
     4501 gtctcctgtt ccgccgccaa ctgcagttaa tgagccaagc atcagattgt gtacgcaggc
     4561 aataccatcg gtcgccatac gaacgctgac cggtgagagc ataatgttgt tatcgatcag
     4621 tgtcggacca tggccaacct cgatgaaaat atcctgactc atcattgcgc cctctgcctt
     4681 tggtgttccc tccggcgggc agttgtcgtg cagcaggttc tgggtcaggc gtgttccctg
     4741 tgcctcccag tcgcaccaga tgcccatcgt acaatgatga atgtgatttc ttctcatcac
     4801 aacatcgatc gctgcatgca tcttgatacc ggaaatctcg gctccgccaa gctgctgcat
     4861 attgttgatg ttgtgaatgt ggttgtcctc aatgatactg aacacaccgc ccatccgtcc
     4921 gacaataccg gtctgctcac agtgatggat gtggcagcgg cggatgatat ggcttccgat
     4981 gttctccttc gtccagccgt gatactggcc gcggcacaca gcatcacgct ccatctgggt
     5041 cgggcttttt acgtgttttc tggtaaagta atggtcgttc tccggatcgt aatattttcc
     5101 gagcgaaata ccgcagcact tgctgttgct gacctcacag tcctcaataa tccatccctt
     5161 cgaccagtgc ggaccgatca taccgtcctg ataagcagcc ggcggcgccc atgtcgttgc
     5221 tgccttgctg acatcaaatc cactgaacgt aatgtaattt acgcctgttt tcgacggcat
     5281 aaagcagttc cggcgcacgt tgatttctac tttttcctcc gtcgggtttt taccctggaa
     5341 atttgcgtag atgaccgtct gatttttctc tttgtcctgc tcggtatacc atttgtaaac
     5401 cgaccactcc ggctcccagg acggcgcata aacctcgccc ttgatacact cctcgagcgt
     5461 ctctgcctcg tataactggc ggtcattcag ataaaccgcg ccggtatggc gtaccaccgg
     5521 tgcaaaatac cagtcaccgc cgaccattgt cgtgtatgga ttataattgc caaaaactcc
     5581 gttgtctaca cggcataccc acacattatc tttgtaatgc tcccagtcat tcatcgtttc
     5641 cgcaccggtg atctttgcgc cgagcggctt ctcactctta tagacgattc tcgcatcctc
     5701 ggttcctccg ttgaccggat cgacatattc gtggtagata ccaggtgcaa ccagaacttc
     5761 atctcctgcc tttgcaattt ttgccgcatc gttgatgaac ttgaacggca tctcttttgt
     5821 tccgtttcca ttcttcggag cggaattatt gacatagtaa atcatttctc tatactcctt
     5881 tttgttatag taaaacccga aaacactgca aaaaaggctg tgacgcaatc attcactctc
     5941 tgcggcgctg aattttttac gctatttttt tgcgggtcta tagaatttct ttacgataat
     6001 gcttcttccg cagcctcttt tgatgcaaac gggcccggca gcggatgaat gccctgacgg
     6061 tttccgaagt agtcatatcg gaacacgatc ggcgttccat ccggattctc atatttctgt
     6121 tccggctcaa atgccatgcc aagcgtctcc gttgagatca ctgcacaggc attctgcggc
     6181 agataatcgt aaacattggt ctccagtttc cattttccgt tctcttcttt tacaccgatg
     6241 gtgatttcat gctcggtatc cactacagcg tcctgttcct gctctgccgg cttcgcccca
     6301 ttgaagtaaa cgttgccgcc aagccatacc ggcagcttct cgtaatacag atccggtgac
     6361 tttccggagc ccattccgca atagccctca aatcttgcca cgtactcctc caatgtcgga
     6421 tatccgctgt acggtgcggt tcctgctgtc agatttccgt catcccactc attatctccg
     6481 gtcagtttac ggatttcttc cattcccgca cgaaccggct tctggatgaa gatattattg
     6541 tagaaccggc agtcaccgtg aagaaccgtc ataaatccgt tgatctctgt gcgatgcggc
     6601 acatggtacg gtgtgtagcg tggcgacggc agcttttcgg agccattgtt gacaccgcgg
     6661 ccgacagcag taaacgatcc cgcgatcagg ttatgtacta ccgcaacacc ctgcgttgcc
     6721 agcttcatcg cgcggtccga aagcagtacg ttattgtcca ccagtgtcgg tccgtgggaa
     6781 atttcaataa agatatcctc gccgataccg tccatacctt ctggatttgc attttcctcg
     6841 ttcggcagcg tattgtcatg gaacaggttc tgcgtaacac gtgtgccctg tgcctgccag
     6901 tcgagccaga ggcctctcgt acagtgatgg aaatggtttc ttcgaatgat cacatcgatt
     6961 gctgcatgca tcttgatgcc gccgatctcc gcacccgcaa ggttctgttt attattgata
     7021 tggtgaatgt ggttatcctc aataatcgag aacacaccac ctaagtggcc tacaataccc
     7081 gtctgtccac agtcgtggat gttgcaccgg cggatgatgt ggcttccgat gttttccttt
     7141 gtccatccct ctcgctgcgc ctggcagata cagtcacgct ccgtctgtgt accatctttg
     7201 aatttccatt tgagccattt attgtcattg ttcggctgtc tgtattttcc aagggaaatg
     7261 cccgagcact tcgaatctga aatctcacaa tcctcgatga tccatccctt tgaccagtgc
     7321 gggccgacca ttccttcctg atatgccgtc ggcggagccc actgggttgc cgcctgtttt
     7381 acaacaaagc cggacagcgt gatataaccg atgccctcct tcgatggata aaagcaattc
     7441 tcgcgcacat taatttccac cgtttcctca ttcgggtttt tcccctggaa attcacatag
     7501 aaaacggtct cattcttctc ttcgtcctgc tccacatacc aggtgtaaac cgtgaatgcc
     7561 tgatcccagg att
//
LOCUS       DAOMUF010000101         7374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2636_length_7374_cov_3.687116, whole genome shotgun sequence.
ACCESSION   DAOMUF010000101 DAOMUF010000000
VERSION     DAOMUF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2636_length_7374_cov_3.687116"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /gene="rimI"
                     /locus_tag="PLN77_06810"
     CDS             complement(<1..377)
                     /gene="rimI"
                     /locus_tag="PLN77_06810"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRM44022.1"
                     /translation="MITIREMQIDDLEQVMPIEEANFSIPWTETGFFTFLIRDDALFL
                     VAEEDGEILGYLGILISFDESEITNVCVAEKARRRGIGHALMEELFRRMQERKVRVIH
                     LDVRIGNTPARNLYESLGFVQDGL"
     gene            complement(374..1126)
                     /gene="tsaB"
                     /locus_tag="PLN77_06815"
     CDS             complement(374..1126)
                     /gene="tsaB"
                     /locus_tag="PLN77_06815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRM44023.1"
                     /translation="MKILALDSSGLVASVAVVENDTLLAEYTVNYKKTHSQTLLPMLD
                     EISNMTELDLSTIDAIAVAGGPGSFTGLRIGSATAKGLGLALEKPLISIPTTAAIAYN
                     IWDTNKFVCPIMDARRNQVYTGVYCYTDHRLDTVWEQDTMSIEALAEGLNCLDHEVIF
                     LGDGVAVYREKLQELLTIPFSFAPAHVNRQRAAAVGALAEVYYKEGKIQTAEEHEPEY
                     LRKSQAERERAERESVKHADVVKKQENGEKSE"
     gene            complement(1245..1664)
                     /gene="tsaE"
                     /locus_tag="PLN77_06820"
     CDS             complement(1245..1664)
                     /gene="tsaE"
                     /locus_tag="PLN77_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRM44024.1"
                     /translation="MIIETNSASETLALGEKLGKAAKPGQIYTLNGDLGVGKTVLTQG
                     FAKGLGITEAVNSPTFTIIQEYTEGRLPFYHFDVYRIGDIEEMEEIGYDDYFFGEGVC
                     LIEWAELIEELIPEGAISITIEKDLEKGFDYRKITVE"
     gene            1922..3124
                     /locus_tag="PLN77_06825"
     CDS             1922..3124
                     /locus_tag="PLN77_06825"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRM44025.1"
                     /translation="MLTVMKFGGSSVADLAHIRNVAQRCIEKQQAGSQVVVVLSAMGK
                     TTDGLIATAKQITEKPSRREMDMLLATGEQVSVSLMAITMIQMGVSAVSLNAWQVPMH
                     TTSDYQNARFKRIDTERIRKELDDNKIVIVTGFQGINKYDDITTLGRGGSDTTAVALA
                     AELHADVCEIYTDVDGVYTADPRIVPNARKMEEVTYDEMLEMASLGAKVLHSRCVEIA
                     KKYGVELLVCSSLNRNSGTMVREKTKMEKMLISGVAADKNVAKIRVAGLGNDPESTFR
                     LLNLLDRNNLNIDVMIQSLGKDGTKSVSFTVAKPDMLRTIDVLNDNKDSLGIKKLDYD
                     ENVAKISVIGAGVRSHSGVAAKMFGALANAGINTEMVTTSEIRITALIDEAYADQAMR
                     AIHETFLE"
     gene            3284..3802
                     /locus_tag="PLN77_06830"
     CDS             3284..3802
                     /locus_tag="PLN77_06830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM44026.1"
                     /translation="MSNPIVTITMENGDVMKAELYPEIAPNTVNNFISLVKKGFYDGL
                     IFHRVIPGFMIQGGDPAGTGAGGPDYCIKGEFSQNGFENNLAHTPGVLSMARTMFPDS
                     AGSQFFIMHKAAPHLDGAYAAFGKVTEGLEVVDKIASVNTDYSDKPLQPQRMATVTVE
                     TFGVEYPEPEKC"
     gene            3805..4335
                     /locus_tag="PLN77_06835"
     CDS             3805..4335
                     /locus_tag="PLN77_06835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM44027.1"
                     /translation="MNDELMLVEPSEEYFAELAAYRNDFLENSDSMDGCGPLRRFDDM
                     KAYLEDTMRYTREETLPEGMVLATQFLCIRKSDNRLVGMIQVRHYLNDFLRTLGGHIG
                     YSVRPSERRKGYASWMLKNVQPYCKSLGLSEILVCCLDTNEGSRRTILKNGGRFDGTA
                     YRADENKTLERYWITL"
     gene            4809..6068
                     /locus_tag="PLN77_06840"
     CDS             4809..6068
                     /locus_tag="PLN77_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HRM44028.1"
                     /translation="MSKVMIIGCGGVASVAIHKCCQNSEVFSEIMIASRTLSKCDALK
                     EKLAPTTKTIITTAKVDADCTEELIALIKQYQPDAVLNLALPYQDLTIMDACLACKVP
                     YIDTANYEAENTDDPAWRAIYEKRCEELGFSAYFDYSWQWAYMDRFKEAGITGLLGTG
                     FDPGVTSVFAAYAQKHYFDEIHTIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     ENGHWVEIPPMSIKREYNFDEVGEKDMYLLHHEEIEALAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLSTTPITFNGQQIVPIQFLKELLPDPSTLGPRTVGKTNIGCIFTGI
                     KDGKERSIYIYNVCDHQECYREVESQAISYTTGVPAMIGTMMVVSGLWNKPGVYTTDE
                     FDPDPYMEALNKWGLPWKVVENPVLVD"
     gene            6073..7206
                     /gene="nspC"
                     /locus_tag="PLN77_06845"
     CDS             6073..7206
                     /gene="nspC"
                     /locus_tag="PLN77_06845"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRM44029.1"
                     /translation="MYKINELPTPCFVIDEGKLEENLKILHQVEERTGAKILLAQKCF
                     SCFSEYPLIGKYISGTTASGLYEAQLGKEEMGLENHVYSPAYRPQDMEELAQICDHII
                     FNSEKQLRTYLPVLKASGTAKAGLRINPECSTQEGHAIYDPCAPGSRMGIRACDFTDE
                     LADLVDGLHFHTLCEQNSDDLETTLRAVEEKFGKWLFKMNWLNMGGGHHITREDYDIE
                     RLISCINHMKETYGLQIYLEPGEAVALNAGYNVTEVLDIVENNGKILILDTSAACHMP
                     DVLEMPYRPPLKDSGLPGEKPYTYRLSCNTCLAGDVIGEYSFDSQIRIGDRLYFEDMA
                     IYSMVKTNTFNGIPLPSIAVMDKAGNCRMIKEFGYEDFKNRLS"
     gene            7178..>7374
                     /locus_tag="PLN77_06850"
     CDS             7178..>7374
                     /locus_tag="PLN77_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRM44030.1"
                     /translation="MKISKTDCLRTPKADGFYMPGEFEPHEGTLMIWPWRPGSWNYGA
                     KAARAAFAEIAEAIAAYEQVYL"
BASE COUNT         1949 a         1899 c         1696 g         1830 t
ORIGIN      
        1 aggccgtcct gcacaaagcc aagactctca tacagattcc gcgccggcgt attgccgatg
       61 cgcacatcca gatgaatgac gcgcaccttt ctttcctgca tccgacggaa cagctcttcc
      121 atcagtgcat ggccgatgcc gcgtcttctc gctttttctg ccacgcagac atttgtgatc
      181 tcactttcat caaaggaaat caggattccg agatatccta aaatttcacc gtcctcctct
      241 gctacaagaa acagtgcgtc gtcacggatc agaaatgtaa aaaatccggt ttctgtccac
      301 ggaatcgaaa agttggcctc ctcaatcggc atcacctgct ccagatcatc gatctgcatt
      361 tcacggattg tgatcattcg cttttttctc cgttctcctg ttttttcacc acatcggcat
      421 gcttcacaga ttcccgctcc gctctttcac gctccgcctg cgattttctc aggtattccg
      481 gctcgtgctc ttctgccgtc tgaatcttac cttctttgta ataaacctca gccagtgctc
      541 cgacggctgc cgctctctgg cggttcacgt gcgccggtgc aaacgaaaac ggaatcgtaa
      601 gaagctcctg caatttttcc cggtaaaccg caacgccgtc ccccaggaaa ataacctcat
      661 ggtcaagaca attcaggccc tccgccagtg cctcaatgct catcgtatcc tgttcccaga
      721 ccgtatcgag acgatgatcc gtgtagcagt aaactccggt atagacctgg tttcttctcg
      781 catccatgat cgggcagaca aatttgttag tatcccagat attgtacgcg attgccgccg
      841 tcgttggaat ggaaatcagt ggtttttcaa gtgccagacc aagtcccttg gctgttgcag
      901 aaccgatgcg aagaccggta aacgagcccg gacctccggc aactgcaatc gcatcgatcg
      961 tcgaaagatc cagctctgtc atgttcgaga tttcatccag catcggaagc agagtctggg
     1021 aatgtgtttt tttataattt acggtgtatt ccgcaagcag tgtgtcattt tcaacaacag
     1081 ccaccgaagc caccaggccg gagctgtcca gtgcaagtat tttcatagtt ttttatttcc
     1141 tttccgatat ttccttcctg tgcaactcat tttggcagtc tgccggaaac aggaatcttt
     1201 acgatttcct tttgtttttc caccacattc aggatttttt tcttttattc caccgtaatt
     1261 ttccggtaat caaacccttt ctcgaggtct ttttctatcg taatcgaaat cgcaccctcc
     1321 gggatcagct cttcaatcag ttctgcccat tcgatcaggc agacaccctc accgaaaaag
     1381 taatcatcgt atccgatttc ttccatttct tctatatcac cgatccggta aacatcgaaa
     1441 tgataaaatg gcagacgtcc ttcagtatat tcctgaataa tggtaaatgt gggactgttg
     1501 actgcctctg tgatgccaag tccttttgca aagccctgcg tcagaacggt ttttccaaca
     1561 ccaaggtctc cgtttaatgt ataaatctgg ccaggcttcg ccgcttttcc cagcttttcg
     1621 ccaagggcaa gcgtctccga cgcagaattt gtttctatga tcatatgctt tcttctcctc
     1681 tttttctgcc tatcatttta acacaaacaa aaaggtaata caaccgcaga acgaaaccgc
     1741 ttttccttgt gcttttcctt gtgcttttcc ttgctttttc tatctgtttt cgctatacta
     1801 tgtatcagag tttcgcggcc caaaaacgtc ccactcctgt tttttcggcc cggaaaacgc
     1861 ggtaaaacac gctctgcgct tttgtccgcg taaatcaaaa aggaaagaga tcaaaactgt
     1921 tatgctgact gtaatgaaat ttggcggaag ctccgtcgca gatcttgcac acatccgcaa
     1981 tgtagcacag cgctgcatcg aaaaacagca ggccggaagc caggtcgttg tcgttctctc
     2041 cgcgatggga aaaacgacgg acggactgat tgcaaccgca aaacagatta cggaaaaacc
     2101 gtcccgccgc gagatggata tgcttcttgc caccggtgaa caggtttccg tttccctgat
     2161 ggccataacc atgattcaga tgggcgtcag tgctgtttct ttaaatgcct ggcaggtacc
     2221 gatgcacacg acctccgatt atcagaacgc gcgcttcaaa cggatcgaca ccgagcgcat
     2281 ccgtaaagag ctggatgaca acaaaattgt cattgtcacc ggcttccagg ggatcaacaa
     2341 atacgatgat atcacaacgc tcggacgcgg cggctccgat acgactgcgg ttgcccttgc
     2401 ggcagagctg cacgcggatg tctgtgaaat ctacacggat gtagacggcg tctacaccgc
     2461 agatccgcgg attgtaccga atgcgcgcaa aatggaagag gtaacttacg atgaaatgct
     2521 ggagatggct tcgctcggcg caaaagtcct tcacagcaga tgcgtggaaa tcgcaaaaaa
     2581 atacggcgtg gaacttctgg tctgctccag tctgaaccgg aattccggaa cgatggtaag
     2641 ggagaaaacc aaaatggaaa aaatgttaat cagcggcgtg gctgccgaca aaaatgtggc
     2701 aaaaatccgt gttgcaggcc ttggcaacga tccggagagc acgttccgcc tcttaaattt
     2761 actggatcgg aacaatctga acatcgatgt catgatccag tcactcggaa aggacggcac
     2821 aaagtccgtt tcctttaccg ttgcaaagcc ggatatgctg cgcacgatcg atgtgctgaa
     2881 cgacaataag gattcgctcg gcatcaaaaa gctggattat gatgagaacg tggcaaagat
     2941 ttccgtcatc ggagccggtg tccgctccca ttcgggcgtg gctgccaaaa tgttcggcgc
     3001 gctcgccaat gcaggaatca ataccgagat ggttaccacc tcggaaatcc gcatcactgc
     3061 cctgatcgac gaagcatacg cagatcaggc gatgcgtgcg atccatgaga cattcctgga
     3121 gtaatgatct gaaaaaaggg gtttgccccc tttgatacca gatcgctacg tgctttgcac
     3181 tccagcaatc atcaaaaaca ttcgtaatcc gctcattttt cttcgaaaaa tggctactta
     3241 cgtcttatca aaaaatcgta aaaaaatcag gaggaaaaca attatgtcta acccaatcgt
     3301 tacaattact atggaaaacg gcgatgtgat gaaagcagag ctttacccgg aaatcgcgcc
     3361 gaacaccgta aacaacttca tcagtctggt aaaaaaaggc ttctatgacg gcctgatctt
     3421 ccaccgcgtc atcccgggat tcatgatcca gggcggagat cctgcaggca caggcgccgg
     3481 cggcccggat tactgcatca agggtgagtt ctcccagaac ggatttgaga acaacctcgc
     3541 acacacccca ggcgttctct ccatggcacg taccatgttc ccggacagcg caggaagcca
     3601 gttcttcatc atgcacaaag ctgctccgca tctggacggc gcatacgctg ctttcggaaa
     3661 agtaacagaa ggcctggaag tcgtagataa aatcgcatct gtcaacaccg actacagcga
     3721 caaaccgctg cagccgcaga gaatggctac cgttaccgtt gagaccttcg gcgtcgagta
     3781 tccggaaccg gagaaatgct aaggatgaac gatgaactga tgttagttga accgtcggag
     3841 gaatacttcg ccgagctcgc cgcctaccgg aacgattttc tggaaaacag cgattccatg
     3901 gacggctgcg gaccgcttcg ccggtttgat gatatgaagg cgtatcttga agataccatg
     3961 cggtatacca gagaggagac tctgccggag ggcatggttc tcgcaacaca gtttctgtgc
     4021 atccgcaaat cagacaaccg ccttgtcggc atgattcagg tgcgccacta cctgaacgat
     4081 tttctgcgca ccttgggcgg ccacatcggc tactccgtcc gcccgtcgga acgcagaaaa
     4141 ggctatgcct cctggatgct gaaaaatgtg cagccgtact gcaaaagcct cggcctttcc
     4201 gaaattctgg tctgctgcct ggataccaac gaaggaagcc gcagaacgat cctgaaaaat
     4261 ggcggccggt tcgacggcac cgcttatcgt gccgatgaaa acaaaacact ggagcgttac
     4321 tggatcaccc tgtgattttt cttccatttc gcatggctct gaatagtttc acaaattttt
     4381 cttgacatcc atgtcatttc actttataat ttggaactgc aagcgaacaa ttctgttctt
     4441 atgcaaaata tatgcccctg gtaaaaaacc gacggacagg caggggtagt tgccgagcgt
     4501 ggttaagagc cacattattg attgcgttaa aagcgcaaat tttatgagtt gattacttta
     4561 taatcgatgc tctacgacga gcacatcatc ttatgaaacg gtcaataaga gtacgtgact
     4621 atatatttct gcataggaaa acagggggaa gcctggattt ttgtccatcc cctgaagttt
     4681 gcacaatacg atcatgtata aatttcgatt tttagacaga tgatgcacgt catctgtctt
     4741 tttttcgtgc ttagataacc tgccaaagat gaacccaaca ccattttctg aaaaaggaga
     4801 accaaacaat gtccaaagtt atgatcatcg gatgcggcgg tgttgcatcc gtagccattc
     4861 acaaatgctg ccagaacagt gaagtatttt ctgaaattat gattgcaagc cgcacccttt
     4921 ccaagtgcga tgctttaaaa gaaaagctgg ctccgaccac aaaaaccatt atcacaacag
     4981 caaaggtgga tgccgactgc acagaagaat taatcgcctt aatcaaacag tatcagccgg
     5041 atgccgtatt aaatctggct cttccttatc aggatcttac cattatggat gcctgccttg
     5101 catgtaaggt tccttacatt gatactgcaa attacgaagc agaaaatacg gatgatccgg
     5161 catggcgtgc aatctacgaa aagagatgcg aagagcttgg cttttctgct tactttgatt
     5221 actcctggca gtgggcctat atggaccgtt tcaaggaagc cggcatcacc ggtcttctcg
     5281 gaaccggctt tgacccgggc gtcaccagtg tctttgcagc ctatgcacag aaacactatt
     5341 tcgatgaaat tcacacgatt gatattctgg actgcaacgg cggcgaccac ggatatccgt
     5401 ttgcaaccaa cttcaatccg gaaatcaacc tccgcgaggt atccgctccc ggctcttact
     5461 gggaaaacgg ccactgggta gaaattccgc caatgtcgat caaacgggaa tataacttcg
     5521 atgaggtagg cgagaaggat atgtaccttc ttcaccatga agaaattgag gctcttgcaa
     5581 aaaatatccc gggcgtaaaa agaatccgct ttttcatgac cttcggccag agctatctga
     5641 cccatatgaa atgtctggaa aatgtaggac ttctctccac cactccgatt accttcaacg
     5701 gacagcagat cgtaccgatc cagtttttaa aagagcttct cccggatcct tccacccttg
     5761 gcccgcgcac cgtgggtaaa accaacatcg gctgtatttt taccggtatc aaggacggaa
     5821 aagaaagaag catttacatt tacaatgtct gtgaccacca ggaatgctac cgggaagtag
     5881 agtctcaggc gatctcctac accaccggcg ttccggccat gatcggaacc atgatggtcg
     5941 tgagcggtct ctggaacaag ccgggtgtct acaccacaga tgaattcgat ccggatccgt
     6001 atatggaagc actcaacaag tggggtcttc cgtggaaggt cgtagaaaat cctgttttag
     6061 tagattaagc ctatgtataa gataaacgaa ctgccaactc cctgttttgt cattgatgaa
     6121 ggcaaactgg aagaaaactt aaagatcctg caccaggtag aagaacgcac cggtgcaaaa
     6181 attctgctgg ctcagaaatg cttttcctgt ttttcggaat atccgctgat tggaaaatat
     6241 atcagcggaa cgactgccag cggactctat gaggcgcagc ttggcaagga agaaatgggg
     6301 ctggagaatc atgtgtattc tccggcctac cgcccgcagg atatggaaga gctcgcacag
     6361 atctgcgatc atattatttt caattcagaa aagcagctcc gaacgtacct ccccgtactt
     6421 aaggcttccg gaaccgcaaa agccggactt cgcatcaacc cggaatgctc cacgcaggaa
     6481 ggccatgcga tttatgatcc ctgtgcgccc ggctcccgca tgggcatccg cgcctgcgat
     6541 tttacagacg aactggccga tcttgtggac ggtcttcatt ttcataccct ctgtgaacag
     6601 aattcggatg atctggaaac cacgctgcgc gccgtggaag aaaaattcgg caagtggctt
     6661 tttaaaatga actggcttaa catgggaggc ggacatcata tcacccgtga ggattacgat
     6721 atcgaacgcc tgatttcctg tatcaaccat atgaaagaaa cgtatggtct tcagatttac
     6781 ctggagcccg gggaagcagt tgccttaaac gcaggctaca atgtcacgga agttctcgat
     6841 attgttgaaa ataacggaaa aattttaatt cttgacactt ccgctgcctg ccatatgccg
     6901 gatgtgcttg agatgccgta ccgccctccg ctcaaagaca gcggactgcc cggggaaaag
     6961 ccttatacct accgcctttc ctgcaatacc tgcctggcgg gcgacgtaat cggcgaatat
     7021 tccttcgatt cccaaatccg gatcggtgac agactgtatt ttgaagatat ggcgatttat
     7081 tcgatggtaa aaaccaatac ctttaacgga atcccactcc cttccatcgc ggtgatggac
     7141 aaagctggaa actgccgaat gataaaggag tttggatatg aagatttcaa aaaccgattg
     7201 tcttagaacc ccaaaagccg atggctttta catgccgggc gaatttgagc cccacgaagg
     7261 cacgctgatg atctggccgt ggcgccccgg ctcctggaat tacggagcaa aagcggcaag
     7321 agcggcattt gcagaaattg cagaagcaat tgccgcttat gagcaggttt atct
//
LOCUS       DAOMUF010000102         7334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2653_length_7334_cov_3.903696, whole genome shotgun sequence.
ACCESSION   DAOMUF010000102 DAOMUF010000000
VERSION     DAOMUF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2653_length_7334_cov_3.903696"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="PLN77_06855"
     CDS             <1..140
                     /locus_tag="PLN77_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HRM44031.1"
                     /translation="VQATYYHTEVVPAMDALRAPVDKLEMIVDKEMWPMPSYGDLMFE
                     V"
     gene            245..1033
                     /locus_tag="PLN77_06860"
     CDS             245..1033
                     /locus_tag="PLN77_06860"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRM44032.1"
                     /translation="MPEEKGGQRCRNKRQENMEETLLEFRRKTEAYRTMNAELEILGV
                     KLKHYRVDAAMEIAEEYLSNDKLNTIGIVTMQMLMQASKEPTWKQYIEDLDLTVIGET
                     EVLEAAGIDPETPSYEEVETNEFIARFFWGLIQTKSRIFVLGDTEEELQTLQKYLTET
                     YPGIEIGGGAVVETLEESAFDSLVNEINSQTTDVIVSGLAGTRQDRFVLENSSKISAK
                     IWLSLGEHSTLQNEAGIKTSWWGTLLKKNTFKRMVTKYNSEKDG"
     gene            1039..1908
                     /locus_tag="PLN77_06865"
     CDS             1039..1908
                     /locus_tag="PLN77_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM44033.1"
                     /translation="MNWIRNKQQWRDYLLITLGTAVMAVATSSIYDPAGLVTGGFSGL
                     AIVIKQLSQMLFGKGVPLWVTNLVLNIPLFLFGIRMSRDFLVKTIFGTLMNTFWIAVL
                     PVLQLVPDDRMLSALFGGVLMGAGIGLVFIGNGTTGGTDMMAALIQRKLRHYSVAQVM
                     QVLDGIIVIAGIFVFGIHASLYAIVAIYVTTKVTDALMEGMNYAKSAFIITAHPEQVA
                     DELMKELDRGASGIHIKGMYTGEERMMVFCVVGKKQIPQLKQIVGTIDPDAFVVVSDA
                     REVLGEGFQTDFS"
     gene            2094..3182
                     /locus_tag="PLN77_06870"
     CDS             2094..3182
                     /locus_tag="PLN77_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM44034.1"
                     /translation="MISNQILQSTIEGIKNISRVELCVTDVEGKVLVTTFPEVEISQN
                     DIVTFAQSQADSQTMKNYQFFKVYDEHQLEYILVVKGGNDDIHLIGKMVTFQIQGLLT
                     AYKERFDKDNFIKNLLLDNLLLVDIYNRAKKLHIEVEARRLVFILETNPDKNSGALES
                     VKSLLGTKSGDFITAVDEKNIIIVKELAPGEDYAQMNKVAATILEAAGRDEEGKTHVA
                     YGTIVKEIKEVSRSYKEARMAMDVGKIFSAENDIYAYSSLGIGRLIYQLPIPLCKMFI
                     KEIFGNKSPDDFDEETLTTINKFFENSLNVSETSRQLYIHRNTLVYRLDKLQKSTGLD
                     LRGFEDAITFKIALMVVQYMKYMETLDY"
     gene            3209..3892
                     /gene="ftsE"
                     /locus_tag="PLN77_06875"
     CDS             3209..3892
                     /gene="ftsE"
                     /locus_tag="PLN77_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRM44035.1"
                     /translation="MITLDSVSKSYAKGQPAVNNVSLHIEKGEFVFIVGNSGSGKSTL
                     IKLLLKELEPTSGTIKVNGQILNKMKRWRVPKYRRGVGVVFQDFRLLKDRNVYENVAF
                     AQRVIEKPNRVIKKRVPEVLTLVGLAEKYKSFPKELSGGEQQRVAVARAIVNRPDILL
                     ADEPTGNLDPGNSMEIMKLLEEINNRGTTVLVVTHNREIVNSMKKRVITMRKGVVVSD
                     EKEGEYHEV"
     gene            3882..4796
                     /gene="ftsX"
                     /locus_tag="PLN77_06880"
     CDS             3882..4796
                     /gene="ftsX"
                     /locus_tag="PLN77_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRM44036.1"
                     /translation="MRFSTLGYSIKQGVKNIWRNKMFSLASIATMGACIFLFGIFFAV
                     VINFNYIVKNAETDVSMTVFFNEDVDQATIDEIGTEIKAKTDVVKECKYVSADETWEN
                     FAKQYFEGKEEYKDGFKPNDNPLATSAHYEVYVYQVETQDALAEYIRSLDGVREVKQA
                     EGATTTLTTMNRVIGYVSIAIILILLAVSVFLISNTVTIGISVRKEEIGIMKLIGATN
                     LFVRAPFLIEGILIGLVGAAIPLGLLYVVYNRVISYVMTEFSVLSNFMAFMPVSAVFR
                     ILLPVGLILGMGIGLVGSIFTIRKHLKV"
     gene            4880..6139
                     /locus_tag="PLN77_06885"
     CDS             4880..6139
                     /locus_tag="PLN77_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRM44037.1"
                     /translation="MEELMKEEKEGVKAAEQEKPKKRRNRKKNRKNEMRKSYFAGMIS
                     MLALVMIVLVGTTFLWPGSSADHPGGFWAWRKTAAIKAMISQLYAGDVDDTELSDAMY
                     KGLISGLGDKYSTYYTKEEYEAIKTAQEGYYQGIGVTVTLDGDKLQVTGVQDDTPAAK
                     AGIQADDRIVKIDGQTLEGLSLNDAVGLLKNSENDTVTLSIEREGEADLIETEVTKEK
                     LAAIVADGKMLDDKIGYLAISEFTGLTSEQFQKVYQSLQDQGMERLIIDLRGNPGGLV
                     TAVCDTLRQILPEGLIVYTEDKNGKREEYTCDGDTPISIPLVVLVNENTASAAEIFTG
                     AVKDYGIGTIVGTTTFGKGIVQNTFQLSDGSVVKLTIAHYYTPLGNDIHKVGITPDVE
                     VELPDDATSDVQLEKALEVVKGLESAE"
     gene            6259..>7334
                     /locus_tag="PLN77_06890"
     CDS             6259..>7334
                     /locus_tag="PLN77_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRM44038.1"
                     /translation="MNFTERRKQVFDKMDQDSALLLFSGIELHVSADEYLPFEANRNF
                     FYLTGLRRDHMILMMKKTAKEEKSILFIEEADLNQERWFGRKVTVSEAQEITGIDDVR
                     FLDSFEGMVNRLMAREDIGTLYFDCYRYQVEDLPDYNMVKAEEFAKKFPGRPIKNIAP
                     VIAALRMQKDEDEIALVRQAIKITDDALKFVMKNLKPGCTEYQAQADFEYKIFHEGAD
                     GTAFPTIAGSGANGTMLHYDTNRDVCEDETLLLMDLGAKFQGYCADITRTYPVNGKYT
                     DRQRQVYDVVLAANRAVAKAAKPGMTLKELDDIAKDVLGEGCVKLGLIEKKEDVGTYY
                     MHGVSHHLGIDVHDVTAAWNDKLRP"
BASE COUNT         2087 a         1613 c         2034 g         1600 t
ORIGIN      
        1 aagtacaggc tacatactat catacagagg ttgttccggc tatggatgca ctccgtgcac
       61 cggttgacaa actggagatg atcgtagaca aagagatgtg gccgatgccg tcttacggcg
      121 atctgatgtt tgaggtttaa tccgcagacg gtccgataaa ttgtaaaaat tctgtgaaaa
      181 cagggcagaa aaagaggctg tttttgacaa cagcctcttt ttttagtata atggagatta
      241 gttgttgcca gaagaaaagg gcggacagcg ctgccggaac aagcggcagg aaaacatgga
      301 ggaaacgctt ctggaatttc ggagaaaaac agaagcatac agaactatga atgcagaact
      361 ggaaatttta ggtgtgaagc tgaaacatta ccgcgtcgat gcggcaatgg agattgcaga
      421 ggaatacctc tcgaacgata agctgaacac catcgggatt gttacgatgc agatgctgat
      481 gcaggcgagc aaggaaccga cgtggaaaca gtatatcgaa gatctggatc tgacggtcat
      541 cggggaaacc gaggtgctgg aggcagcagg aattgacccg gagacgccgt cctatgagga
      601 agtggagacg aacgagttca tcgcccgttt tttctgggga ctgattcaga cgaaaagccg
      661 gattttcgta ctgggcgaca ctgaggaaga actgcagaca ctgcagaaat atctgacaga
      721 aacgtatccc ggcattgaga tcggaggcgg tgctgtcgtc gagacgctgg aggaatccgc
      781 gtttgacagc ctggtcaacg agatcaacag ccagacgacg gacgtgattg tgagcggcct
      841 tgcgggaacg cggcaggatc gtttcgttct ggaaaacagc agcaaaatca gcgcaaaaat
      901 ctggctgagc cttggcgagc attcgacgct gcagaacgag gccggtataa aaacgagctg
      961 gtggggaact ctgctaaaga agaatacgtt caagcggatg gtaacaaaat ataacagcga
     1021 aaaggacggt tgaagttcat gaactggata cggaacaaac agcagtggag agattatctg
     1081 ctgattacgc tgggaaccgc ggtcatggcg gttgcgacca gctcgattta cgacccggcg
     1141 gggcttgtta ccggaggatt ttccggtctt gcaatcgtca tcaaacaact gagccagatg
     1201 ctgttcggaa aaggagttcc gctctgggtg acgaaccttg tgctgaacat tccgctcttt
     1261 ctgttcggaa tccgcatgag ccgcgatttt ctggtgaaaa ccatcttcgg aaccctgatg
     1321 aatacgttct ggattgccgt tctcccggtg ctgcagctgg tgccggatga caggatgctt
     1381 tccgcactgt tcggcggcgt tctgatggga gcaggcatcg gcctcgtctt catcggaaac
     1441 ggtacgacgg gcggaacgga tatgatggcg gctctgatcc agagaaagct gcgccactat
     1501 tccgttgcac aggttatgca ggtgcttgat ggaatcatcg tcatcgccgg tatttttgtc
     1561 ttcggtatcc acgcttcgct ctatgcgatc gtggcaatct acgtgacgac gaaagtgacg
     1621 gatgccctga tggaaggtat gaattatgcg aaatctgcgt ttatcattac cgcgcatccg
     1681 gagcaggtag ccgatgagct catgaaagaa ctcgaccgcg gcgcgtccgg catccacata
     1741 aagggaatgt acacgggcga agagcgtatg atggtattct gtgttgtggg caaaaaacag
     1801 atcccgcagc tcaaacagat cgtaggaacg atcgatccgg acgcgtttgt cgttgtgtcg
     1861 gatgcaagag aggtcttagg agagggcttc cagacggatt tctcctgacc tttttacgta
     1921 ttttgtggta taaatgttgt gttttctctt ccttttttgt ctattttgta ttaaaataat
     1981 tatatctgtg aggtactttg catcaggcgc agaaccatag gggtgtggat atgcctggca
     2041 atgaaagcgg gtgcagccgg agacaaacta taggatgaaa gagggaagaa aatatgattt
     2101 caaatcagat tttacagagt accattgagg gaatcaaaaa tatttcgcgt gtcgaactct
     2161 gcgtgactga tgtagaagga aaagttctgg taacgacgtt tccggaggtt gagatcagcc
     2221 agaatgatat tgtaacgttt gcgcagtctc aggcagattc ccagaccatg aaaaattatc
     2281 agttttttaa agtatacgat gaacaccagc tggagtacat tctggttgta aaaggcggaa
     2341 acgatgacat tcacctgatc gggaaaatgg ttaccttcca gattcagggc ctccttaccg
     2401 cctacaagga gcgcttcgat aaagacaact ttatcaagaa tctgctgctc gacaacctgc
     2461 tgctggtcga catctacaac agggcgaaga agttacatat tgaagtggaa gcacgccgcc
     2521 ttgtatttat tcttgagaca aatccggata aaaacagcgg ggcgctcgag agcgtcaaga
     2581 gtctgctcgg taccaaatcc ggtgacttta tcactgccgt cgatgagaaa aacatcatca
     2641 tcgtaaaaga acttgctcca ggtgaggatt atgcccagat gaacaaagtg gcagccacca
     2701 ttctggaagc agcaggaaga gatgaggagg ggaaaaccca cgttgcttac ggtaccattg
     2761 tcaaggagat caaagaagta tcgcgttcct acaaagaagc cagaatggcg atggacgtcg
     2821 gaaaaatctt ctccgctgag aatgatatct atgcatacag ctcactgggt atcggccgtc
     2881 tgatttacca gcttccgatc ccactgtgca aaatgttcat caaagagatt tttggcaaca
     2941 aatcgccgga tgactttgat gaagagacac tgacaacgat caataaattc tttgaaaatt
     3001 ctctgaatgt ctcggaaacc tcgagacagc tttatataca ccgaaatacc cttgtctatc
     3061 ggctggataa gctgcagaag agcacgggac tcgatcttag aggttttgaa gatgcgatca
     3121 cattcaagat tgcgcttatg gttgtgcagt acatgaaata catggaaacg ctcgattatt
     3181 aagcgagaac ccttgaagga ggaattgcat gataacattg gatagtgtaa gtaagagtta
     3241 cgcgaaaggc cagccggccg tcaacaacgt atccttacac atcgagaagg gggaattcgt
     3301 ctttatcgtg ggaaacagcg gttctggaaa atcgacgctg atcaagctcc ttttgaaaga
     3361 actcgagccc acatccggta ccattaaggt aaacggacag atcttaaata aaatgaaacg
     3421 ctggagagtg ccgaaatacc gccgcggtgt cggcgtcgta ttccaggatt tccgcctttt
     3481 aaaagaccgg aatgtctatg aaaacgtagc attcgcacag cgtgtcatcg agaaaccgaa
     3541 ccgcgtcatc aaaaagcgcg tgccggaagt cctgacgctg gtcggactgg cagagaagta
     3601 caaatccttc ccgaaggagc tttccggagg agagcagcag cgagttgccg tggcaagagc
     3661 cattgtcaac cggcctgaca ttcttctggc ggacgagccg accggaaacc tggacccggg
     3721 aaactccatg gagattatga agctcctgga ggaaatcaac aacagaggaa cgaccgttct
     3781 tgtggtaacc cacaaccgtg agatcgtcaa ctccatgaaa aaacgcgtta ttaccatgcg
     3841 aaaaggcgtg gtagtcagcg atgagaagga gggcgagtac catgaggttt agtacactgg
     3901 gctacagcat caaacagggt gtcaaaaaca tctggagaaa caagatgttc tcgctggcat
     3961 ccatcgcaac catgggagcc tgtatctttc tgttcggtat cttctttgcg gttgtcatca
     4021 actttaacta catcgtaaaa aatgccgaga cagacgtatc catgaccgtc ttcttcaacg
     4081 aagatgtgga tcaggcgaca atcgatgaga tcggaacgga gatcaaggcc aagaccgatg
     4141 tggtcaagga atgtaagtat gtttccgcgg atgagacgtg ggaaaacttt gcaaaacagt
     4201 atttcgaagg aaaagaggaa tacaaggacg gctttaagcc aaacgacaac ccgctcgcaa
     4261 cttctgctca ctacgaggtt tacgtatacc aggtagagac gcaggatgca ctggctgagt
     4321 atatccgctc gctggacggt gtccgtgagg ttaaacaggc cgagggtgca acaacaacgt
     4381 tgacgacgat gaaccgtgtc attggctacg tttccatcgc gatcatcctg attcttcttg
     4441 ccgtatctgt tttcctgatc agcaataccg tcacgatcgg tatttcggtc cggaaagagg
     4501 agatcggtat catgaagctg atcggtgcga cgaacctgtt tgtaagggct ccgttcctga
     4561 tcgagggtat cctgatcggt cttgtcggag cagcgattcc gctggggttg ctgtacgttg
     4621 tatacaacag agtcatcagc tacgttatga cggagttcag cgtactgtcg aactttatgg
     4681 cattcatgcc ggtatcggcc gtattccgga ttctgcttcc ggtcggactc attctcggta
     4741 tgggaatcgg acttgtcggc agtatcttta cgattcgaaa acacttgaaa gtctgatagg
     4801 attagacaat ataataaagt aaaaataagt cccggaatcc gtcaaaagat tccgggatct
     4861 ttcgattggg aggcatattt tggaagaact gatgaaggaa gaaaaagagg gcgtaaaagc
     4921 ggcagagcag gaaaaaccga aaaagcgcag aaacagaaag aaaaaccgaa aaaatgagat
     4981 gcggaagagc tactttgcgg gcatgatttc catgctggcg cttgtaatga tcgtgcttgt
     5041 cggaacaacg tttctttggc cgggaagcag tgcggaccac cccggcggct tctgggcgtg
     5101 gcggaagacg gcggcgatca aagcgatgat cagccagctt tacgcgggcg atgtggatga
     5161 cacagagctg tcggatgcga tgtataaagg gctgatcagc ggcctcggcg acaagtattc
     5221 tacgtattac acgaaagagg aatacgaagc gatcaagaca gcgcaggagg gttactatca
     5281 gggcatcggt gttacggtga cgctggacgg cgataagctg caggtcaccg gcgtacagga
     5341 cgataccccg gcggcgaagg ccggtattca ggcggatgac cgcattgtga agattgacgg
     5401 gcagacactc gaaggcttaa gcctcaacga tgcggtcggt cttctgaaaa acagcgagaa
     5461 cgacaccgtg acgctttcca tcgagcggga aggcgaggcg gatctgattg aaacggaagt
     5521 cacaaaagag aagcttgcgg cgattgtggc agacggtaaa atgctggacg acaagatcgg
     5581 ctatctggcc atcagcgagt ttacagggct tacctccgag cagttccaga aagtatacca
     5641 gtcactgcag gatcagggca tggagcgcct gatcatcgat ctgcgcggca atccgggcgg
     5701 cctcgtgacg gcagtctgtg acaccctgcg ccagatcctg ccggagggac tcatcgtgta
     5761 taccgaggat aaaaatggaa aacgggaaga gtatacctgc gacggcgata ccccgatctc
     5821 gattccactc gtcgtgctgg tcaatgaaaa tacggcaagt gcggcagaaa tcttcacggg
     5881 agcagtcaaa gattacggca tcggaacgat tgtcggaacg acgacattcg gaaaaggcat
     5941 tgtccagaat acattccagc tctcggacgg cagcgtggtg aaactgacga ttgcccatta
     6001 ttacacgccg ctcggaaacg acatccacaa ggtcggcatt acgccggatg tcgaggtgga
     6061 gctgccggat gacgcgacga gcgatgtgca gctcgaaaaa gcgttggaag ttgtcaaagg
     6121 actggaaagt gcggaataaa ataagaattt tcacagaaaa gcaggaaaaa ggtaggaaaa
     6181 aaagcacgaa cgtgttatct tagtagagga gccgaaaaca gatcttggca ggctctaaaa
     6241 gaaagtgagg aaagacagat gaactttaca gaaagaagaa aacaggtttt tgataaaatg
     6301 gaccaggatt ccgcactgct tttgttttcc ggaatcgagc tgcatgtcag cgcagacgaa
     6361 tatctgccgt ttgaagcaaa ccgcaacttt ttctatctga ccggccttcg cagagatcac
     6421 atgatcctga tgatgaaaaa gaccgcaaaa gaggagaaat ccattctgtt tatcgaggaa
     6481 gcggatctga accaggagag atggtttgga agaaaagtaa ccgtatccga ggcacaggag
     6541 atcaccggaa tcgatgacgt tcgtttcctg gattcctttg agggcatggt aaaccgtctg
     6601 atggcacgcg aggacatcgg aaccctgtat ttcgactgct accgctatca ggtcgaggat
     6661 ctgccggatt acaacatggt aaaggcagag gagttcgcaa agaaatttcc aggcagaccg
     6721 attaaaaaca ttgctcctgt catcgcagca ctgcggatgc agaaagatga ggatgagatc
     6781 gctctcgtcc gccaggcaat caagatcacc gacgacgcgc tgaaattcgt gatgaagaat
     6841 ctgaaaccgg gctgcaccga gtatcaggca caggctgatt tcgagtacaa gattttccac
     6901 gagggagcag acggaaccgc attcccgacg atcgcaggaa gcggtgcaaa cggaacgatg
     6961 ctgcactacg acaccaaccg tgacgtgtgc gaggatgaga ccctgcttct gatggacctc
     7021 ggagcaaaat tccagggcta ctgcgcagat atcaccagaa cgtatccagt caacggaaaa
     7081 tatacggacc gtcagagaca ggtttacgac gttgttctgg cagcaaaccg cgccgttgca
     7141 aaagcagcaa aaccgggtat gacattaaaa gagctcgacg atatcgcaaa ggatgttctc
     7201 ggcgagggct gtgtgaaact tggcctgatc gagaagaaag aggatgtcgg aacctattac
     7261 atgcacggcg tttcccatca cctcggcatc gatgtacacg atgtgaccgc ggcatggaac
     7321 gacaagctgc gtcc
//
LOCUS       DAOMUF010000103         7248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2713_length_7248_cov_2.573057, whole genome shotgun sequence.
ACCESSION   DAOMUF010000103 DAOMUF010000000
VERSION     DAOMUF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_7248_cov_2.573057"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLN77_06895"
     CDS             <1..281
                     /locus_tag="PLN77_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRM44039.1"
                     /translation="TALLTGFMAKESVVSTLSVLFGTTAQLTEILTPVSALSLLVFCL
                     LYTPCVAAVASIKRELGGRWAAGVVVSQCVIAWLAAFVVHGFALLFGM"
     gene            complement(378..>790)
                     /locus_tag="PLN77_06900"
     CDS             complement(378..>790)
                     /locus_tag="PLN77_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44040.1"
                     /translation="VLVKLTHLDPEQKASVRLISVFYIVLILLYIRFIYEDTNYDGLI
                     LYFLTLTVGRFLYFDFTVKSFLETMRGVLENLPLLVIMSAYSGFVCWYGFHVGFLLKS
                     NGVILSTFLAHLFMDCSIFLLHKTGILKHVIRKI"
     assembly_gap    791..800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<801..1154)
                     /locus_tag="PLN77_06905"
     CDS             complement(<801..1154)
                     /locus_tag="PLN77_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44041.1"
                     /translation="MKRYYILHYFDPANVGSFLLVSLLGLILVLALVLLIAKLQIQRQ
                     LKEETEEKAKKIRRKKHQRRQYQIVESFYELVFSGTSILLFLSLYYIIDERLPQISMY
                     WEKYQDVVLLVFIVLS"
     gene            1509..2417
                     /gene="pfkB"
                     /locus_tag="PLN77_06910"
     CDS             1509..2417
                     /gene="pfkB"
                     /locus_tag="PLN77_06910"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HRM44042.1"
                     /translation="MIYTVTFNPSLDYIVSVEDFKLGLTNRTSSELMLPGGKGINVST
                     VLGNLGIESTALGFLAGFTGKEIAGRLDQMGIKNGCIWLEEGYSRINVKLKSIDGTEI
                     NGQGPEIPEKKVEELMKQLSALGEGDVLFLAGSIPSSMADDAYQKIMEMLDGKGVRIV
                     VDATRDLLMKVLPYHPFLIKPNNHELGEIFGVELKTRESVVPYAKQLQEKGAQNVLVS
                     MAGEGAVLVAASGEVFEAPAPKGKLINGVGAGDSMVAGFMAGYMEKEDYLHAFHMGIA
                     AGSASAFSENLATREEIASVYEQITK"
     gene            2433..4346
                     /locus_tag="PLN77_06915"
     CDS             2433..4346
                     /locus_tag="PLN77_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HRM44043.1"
                     /translation="MRITDLLDIRSIALDAAPKSKQETLDAAVALMVKSGKITDEDAY
                     RAQVYAREEESTTGIGEGIAIPHGKCDAVNKPGLAAMVIPKGVDFDSLDGEPVTLLFL
                     IAAPNTKDNVHLDVLSKLSMMLMDEEFTKSLRNARTPEEFLAIIDRADEEKKSVDERL
                     AEPVEAKENQVKILAVTSCPTGIAHTYMAAEGIEKAAKAKGCFVKIETRGSGGAKNVL
                     TAQEIADADCIIVAADAQVPMDRFDGKKVIQRQVSDGISKADELIELAMSGNAPVYKS
                     GNAQTAAASTKKNSGGIGHQLYTQLMNGVSHMLPFVVGGGILIAIAFLIDGLLVDMNS
                     LDAAARSNFGTITPAAAMFKQIGGVAFGFMLPVLAGYIAMAIGDRPALALGFVGGMMA
                     SSGTSGFLGALVAGFAAGYIILGLRKACEKLPEALEKIAPVLIYPVVGILAMGLLMLF
                     IVEPIMGTVNTVLNNGLASMGGSSKLILGMILGGMMAIDMGGPFNKAAYVFGTAAIAA
                     GNYDIMASVMIGGMVPPCAIALATLLFKDKFTKEERESGPTNFIMGLAFITEGAIPFA
                     AADPIHVLPACIIGSGLAGALSMAFGCTLMAPHGGIFVVPVMGNALLYLVAVVIGTVV
                     SAVLLGVLKKKVA"
     gene            4415..4672
                     /locus_tag="PLN77_06920"
     CDS             4415..4672
                     /locus_tag="PLN77_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM44044.1"
                     /translation="MKEFKYVVTDPEGIHARPAGMLVKAVKEFSCDIKIAKDGKAMNC
                     KAIFGIMGLAVKQGEEVTLTFDGADEDAAYEAISNFMKENL"
     gene            4936..6585
                     /gene="ptsP"
                     /locus_tag="PLN77_06925"
     CDS             4936..6585
                     /gene="ptsP"
                     /locus_tag="PLN77_06925"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRM44045.1"
                     /translation="MQIYQGKSVFSGIAIGKIRVYKKNERQVKRVKVENPDAEMARFE
                     EAKAKGIAQLGVLYEKACKEVGEANAAIFEVHQMMMEDDGYNESVENIIKSQGVNAEY
                     AVATTGDNFAQMFSAMDDDYMRERAADVKDISERLISILNGDDTDASLNDEPAIIVAD
                     DLAPSETVQMDKDKVLSFVTVHGSLNSHTAILARTMAIPALVGTPLPLDESVDGKLGI
                     VDGSDGTIYVDPDEETLAAMQKRRTEEEEKKKLLLTLKGKENITLDGQKILLYANIGN
                     IKDLAAVMQNDAGGIGLFRSEFIYLEQDHYPTEEEQFRIYKQAAETMAGKRVIIRTLD
                     IGADKQCDYFEMEKEENPALGCRAIRICLTRPEIFKTQLRALFRASAYGKIAIMYPMI
                     TSVGEVRQIKAIVEEVKASLAEQGIEYGNPEQGIMIETPAAAIISDELAKEVDFFSIG
                     TNDLSQYTLAIDRQNTKLDPFFDPHHPAILRMIQMVVENAHKAGIWAGICGELGADQE
                     LTKEFLAMGVDELSVSPGSILPLRKIILETNVAEYKAGKKC"
     gene            6579..>7248
                     /locus_tag="PLN77_06930"
     CDS             6579..>7248
                     /locus_tag="PLN77_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRM44046.1"
                     /translation="MLTQQRHEIILKLLKEKGSITVTEVRDLLDTSESTVRRDITALD
                     KEGKLEKVFGGAVEAGQKVTAHEYTVAQKNELNCDAKRKIAEYAASLIEPDDFVFLDA
                     GTTTAHMIDFIRATSAVFVTNAVDHARRLASRGFKVILVGGELKSSTEAVVGNQAIRT
                     IQEYHFTKGFFGTNGVTRRSGCTTPDVNEAVTKKTAMEQCRQCYVLCDASKFNSISSV
                     TFADF"
BASE COUNT         2091 a         1718 c         1900 g         1529 t
ORIGIN      
        1 cgaccgccct tctaaccgga tttatggcaa aggagagtgt cgtctccaca ctttccgtac
       61 tgttcggcac gaccgcgcag ctgaccgaaa ttctgacacc ggtttccgca ttaagccttc
      121 tggttttctg cctcctctat acgccgtgtg tggcggcagt cgcttccatc aagcgggagc
      181 ttggcggcag atgggcagcc ggtgttgtcg tttcccagtg tgtgatcgcc tggctggcag
      241 cttttgtcgt acatggcttt gcactgctgt ttggcatgta aaaaagaaca ttaaataaga
      301 aaagctctgc agaacagaaa atgcaaaatt ctgatctgca gagcttttga tatatggctg
      361 gtaaaaaaat gccggtttta aatttttcgt atcacatgct ttaaaattcc tgtcttgtgg
      421 agcagaaaga tcgagcagtc cataaacaga tgggcaagaa aggtgctcaa aatgacgcca
      481 tttgatttca gcagaaagcc gacatggaac ccataccagc acacgaaacc ggagtaggcg
      541 ctcatgatga caagaagcgg tagattttcc aacacgcccc gcatcgtttc aagaaaactc
      601 ttgaccgtaa agtcgaaata caggaagcgg ccgaccgtca gcgtcaggaa atagagaatc
      661 agcccgtcat aattcgtgtc ctcgtagata aagcggatgt acagaagaat cagtacaata
      721 tagaagaccg aaatcagccg caccgacgcc ttctgctccg ggtcaaggtg cgtgagcttt
      781 accagaacca nnnnnnnnnn ggagagcacg ataaagacaa gaagcaccac atcctgatat
      841 ttctcccagt acatcgaaat ctgcggcaga cgctcgtcga taatgtagta gagggagagg
      901 aagagaagaa tcgaagtgcc ggagaaaacc agctcataaa agctttccac aatctgatac
      961 tgcctgcgct ggtgtttctt ccggcgtatt tttttcgctt tttcctctgt ctcctctttt
     1021 agctgccgct gaatctgcaa ttttgcaatc agaagcacaa gggcaagcac cagaatcagg
     1081 ccgagcagtg agacaagcag aaaactgccc acattggcgg gatcaaaata gtgaagaata
     1141 tagtatcgtt tcaaataaaa tcacctcttc tggaaattct ttttttgatt tctcttcact
     1201 ttagtaccaa atccggaaaa agtaaagtac aaaaatgaga acagaaagaa agtgtctgat
     1261 aaaaaatgac cgaaatggag agaataaagt gatcgatgcg tgacaaggaa gaaaagatga
     1321 cgatcagtca aactcggtca aaacagggcg ataaaacggg ggaatccgtc ataaaactct
     1381 aaacttatgc aaatgcgaca aaaagagaag gtaaaattca tgaaatatta atgattgatt
     1441 ttgaacgaat atgacgatat aataagctca tcaaagcaaa tacgatcaaa ataaagcagg
     1501 agggaaatat gatttacaca gtgacattca atccgtctct ggactacatt gtatcggtgg
     1561 aagacttcaa gctcggcctt accaaccgca caagctccga gctgatgctt ccgggcggaa
     1621 aaggaatcaa cgtatcgacc gtacttggaa accttggaat tgagagcaca gcgctcggat
     1681 ttctggcagg ctttaccgga aaagaaatcg ccggacggct cgaccagatg ggaattaaaa
     1741 acggctgcat ctggctggaa gaaggatatt cccgcatcaa cgtcaaatta aagagcattg
     1801 acggaactga gatcaacgga cagggacccg aaatcccgga gaaaaaagtg gaagagctga
     1861 tgaaacagct ttccgcactc ggcgagggcg acgttctctt cctcgcaggc agcatcccgt
     1921 catccatggc agatgatgcc tatcagaaaa ttatggaaat gctcgacggc aagggcgtcc
     1981 gcatcgtcgt agacgcgacc cgtgatctgc tgatgaaggt tctgccgtac cacccgttcc
     2041 tcatcaaacc gaacaatcac gagctcggtg agattttcgg tgtggaatta aagacaagag
     2101 agtcggttgt tccgtatgca aaacagctgc aggaaaaggg cgcacagaac gttctggttt
     2161 ccatggcagg cgaaggagcc gttctcgttg ccgcatccgg tgaggtcttt gaagctccgg
     2221 ccccgaaagg aaagctcatc aacggcgtcg gcgcaggcga ttcgatggta gccggattca
     2281 tggcaggcta catggaaaaa gaagattacc tccatgcgtt ccatatggga atcgcagcgg
     2341 gaagtgcaag tgctttttct gaaaaccttg caacccggga ggaaattgca tctgtctacg
     2401 aacagattac aaaataaaag ggaggacgtc tcatgagaat cacagatttg ctggacatcc
     2461 gcagtattgc actggatgcc gcaccgaaaa gcaaacagga aacgcttgac gcagccgtag
     2521 cgctgatggt caaaagcgga aagatcacag acgaagatgc gtaccgcgca caggtttacg
     2581 caagagaaga agagagcacc acaggaatcg gagagggaat tgcgattccg cacggaaaat
     2641 gtgatgcagt aaacaaaccg ggccttgcag ccatggttat cccgaaaggc gttgattttg
     2701 attctctcga tggagagccg gtcaccctgc tgttcctcat cgcagcgccg aacacgaaag
     2761 acaacgttca tctcgatgtc ttaagtaaac tttccatgat gctgatggat gaagagttca
     2821 ccaagagcct tcgaaacgca aggacaccgg aagagttcct tgccattatc gaccgcgccg
     2881 atgaagagaa aaagagcgta gatgagcgtc ttgcagagcc ggtggaagca aaagaaaatc
     2941 aggtgaagat tctcgccgtt acttcctgcc cgaccggtat tgcccacact tacatggcag
     3001 ccgagggaat cgagaaagca gcgaaagcaa aaggatgctt cgtaaaaatc gagacacgag
     3061 gctccggcgg agcgaaaaac gtgctgactg cacaggaaat cgccgatgcg gactgcatca
     3121 tcgttgcagc cgacgcacag gttccgatgg atcgtttcga cggaaagaaa gtcattcagc
     3181 gccaggtatc cgacggtatc agcaaagcag atgagctgat cgagcttgcg atgtccggaa
     3241 acgcaccggt ttacaagagc ggcaatgcgc agacagccgc agcaagcacg aagaagaaca
     3301 gcggcggtat cggccaccag ctgtacaccc agctgatgaa tggtgtttcc catatgcttc
     3361 cgttcgttgt cggcggcggt attctgatcg caatcgcatt cctgatcgac ggacttcttg
     3421 ttgatatgaa ctcccttgat gccgcagcca gatcaaactt cggtacgatt accccggctg
     3481 cagcgatgtt caaacagatc ggcggcgtgg cattcggctt catgctcccg gttcttgcag
     3541 gctacatcgc catggcaatc ggcgaccggc cggcacttgc ccttggtttt gtcggcggta
     3601 tgatggcatc gagcggaacc tccggtttcc ttggagcact cgttgccggt tttgcagcag
     3661 gctacattat ccttggcctc cgcaaagcat gtgagaaact tccggaagca ctcgaaaaga
     3721 ttgcgccggt tctgatttac ccggtagtcg gcatccttgc gatgggactt ctgatgctct
     3781 tcattgtaga gccgatcatg ggaaccgtca acacggtact gaacaatggt cttgcttcca
     3841 tgggcggatc cagcaagctg atccttggca tgatcctcgg cggcatgatg gccatcgaca
     3901 tgggcggccc gttcaacaaa gcagcttacg tattcggaac agcagccatc gcagcaggaa
     3961 actacgacat tatggcatcc gttatgatcg gcggtatggt tcctccgtgt gcgattgcac
     4021 ttgcaaccct gctgttcaaa gataaattta caaaagaaga aagagaatcc ggtccgacca
     4081 acttcatcat gggactcgcc ttcatcaccg agggtgcgat tccattcgca gcagcagacc
     4141 cgatccacgt tcttccggcc tgcatcatcg gctctggcct cgcaggtgcg ctctccatgg
     4201 catttggctg taccttaatg gcaccgcacg gcggcatctt cgttgtaccg gttatgggaa
     4261 atgcgctgct ttatctggtg gcagttgtca tcggaacagt ggtatccgca gttctgcttg
     4321 gagttttaaa gaaaaaagtt gcataaaaga gtagaaaaaa gacagaaaac aaagtaaaat
     4381 aaaaatatca aagatcctat gggaggaaaa aatcatgaaa gaatttaagt atgttgtaac
     4441 agatccagaa ggaatccacg ctcgtccggc aggaatgctg gtaaaagcag tcaaagaatt
     4501 ttcctgtgat atcaagatcg caaaagacgg aaaagccatg aactgcaaag ccatcttcgg
     4561 catcatggga ctggctgtaa aacagggcga agaagttacc ctgaccttcg atggagcaga
     4621 tgaagatgct gcatacgaag caatcagcaa tttcatgaaa gagaacttgt aattctacag
     4681 agccatgcgg aatggaagaa aaaagtgcga atcatgcttg catgaatgag tgcttttttc
     4741 tggaattcca tatggcgaga agccacatcg aaatggagcg taagcggaat tgagatgggg
     4801 ctctggagaa ttacaatata gtaatcggca ggagcgtgtt tgaaaaactt tctgaaacta
     4861 agaaatcctt tttaaacacg ctccggctgt ctggacagaa aattcggata tcgagaacaa
     4921 aggtggagaa aacttatgca gatttatcag ggaaaaagcg tattttccgg cattgcgatc
     4981 ggaaagatcc gcgtatacaa aaagaatgag cgtcaggtaa aacgtgtgaa agtagaaaat
     5041 ccggatgcgg agatggcacg gtttgaggaa gcaaaggcaa aaggaatcgc acagcttggc
     5101 gttctgtatg aaaaagcatg caaagaggtt ggcgaggcca atgcagcgat cttcgaggta
     5161 catcagatga tgatggagga cgacggctac aacgagtccg tcgagaatat tatcaagagc
     5221 cagggcgtca acgctgagta tgccgttgca acgacaggcg acaactttgc gcagatgttt
     5281 tctgccatgg acgatgatta catgagagag cgcgcggcag acgtaaagga tatttccgag
     5341 cgtctgatct ccatcttaaa cggagacgac accgacgcat ccttaaacga cgaaccggcg
     5401 atcatcgtgg cagatgacct tgcgccgtct gagacggtac agatggataa ggacaaagtt
     5461 ctttcttttg ttaccgtaca cggttcttta aattcccaca ccgctatcct tgcaagaacc
     5521 atggcgattc cggcactggt cggaacaccg cttccgctgg atgagagcgt agacggaaag
     5581 ctcggcatcg tagacggatc ggatggaacc atctatgttg atccggacga ggagactctt
     5641 gctgccatgc agaagcgtcg caccgaggag gaggaaaaga aaaaactgct cctcacctta
     5701 aagggaaaag aaaatattac actggacgga cagaaaattc tgctgtatgc taatatcgga
     5761 aacatcaaag accttgcggc tgtcatgcag aacgacgcgg gcggcatcgg cctgttccga
     5821 agcgagttta tctatcttga gcaggatcac tatccaacag aggaggagca gttccgtatc
     5881 tacaaacagg cagcagagac gatggcggga aaacgcgtca tcatccgtac cctcgatatc
     5941 ggtgccgata aacagtgcga ttatttcgag atggaaaaag aggagaaccc ggcacttggc
     6001 tgccgtgcga tccgcatctg cctgacgcgg ccggagatct tcaagacaca gcttcgcgcg
     6061 ctgttccgcg caagcgcata tggaaaaatt gccattatgt atccaatgat cacaagtgtc
     6121 ggcgaggttc gccagatcaa agcgattgtg gaggaagtaa aagcttctct cgctgagcag
     6181 ggcattgagt acggaaatcc agagcagggt atcatgatcg agaccccggc ggctgccatc
     6241 atcagcgatg agctggcaaa agaggttgat ttcttcagta tcggaacgaa cgacctcagt
     6301 cagtacaccc tcgcgatcga ccgccagaac acaaagctgg atccgttctt tgatccgcat
     6361 cacccggcga tcctgcgcat gattcagatg gttgtggaga atgcacacaa agccggcatc
     6421 tgggccggta tctgcggcga gctcggcgct gaccaggagc tgacaaaaga gttccttgcg
     6481 atgggcgtag atgaactttc cgtttctccg ggaagcatcc ttccgctccg caagatcatt
     6541 ctggaaacaa acgtggcaga atataaggca ggaaagaaat gttaacacag cagagacatg
     6601 aaattatctt gaaactgtta aaagaaaagg gaagcatcac cgtgacggaa gtccgggatc
     6661 ttctcgatac atcggagtcc acggtacgcc gcgatatcac agcgctcgac aaggagggca
     6721 agctggaaaa ggtattcggc ggcgccgtgg aagccggcca gaaggtgacg gcacacgagt
     6781 acaccgttgc gcagaaaaat gagctgaact gcgatgccaa acggaaaatc gcagaatatg
     6841 cggcatccct catcgagccg gacgatttcg tctttctcga cgcgggaacg acaacggcgc
     6901 atatgatcga tttcatccgg gcaacctccg ccgttttcgt gacaaacgcc gttgaccacg
     6961 cacggcggct ggcatcacgc gggtttaagg tcattctggt aggcggcgag ctcaaaagct
     7021 ccacagaagc tgtcgtcgga aaccaggcaa tccgcacgat ccaggagtat catttcacca
     7081 aaggcttttt cggaacaaat ggcgtaaccc gaagaagcgg ctgcacaacc ccggacgtca
     7141 acgaggccgt caccaaaaag accgccatgg aacagtgccg ccagtgctac gtcctctgcg
     7201 acgcaagcaa atttaacagc atcagttccg tcaccttcgc agacttcg
//
LOCUS       DAOMUF010000104         7227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2724_length_7227_cov_3.389013, whole genome shotgun sequence.
ACCESSION   DAOMUF010000104 DAOMUF010000000
VERSION     DAOMUF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_7227_cov_3.389013"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLN77_06935"
     CDS             complement(<1..723)
                     /locus_tag="PLN77_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM44047.1"
                     /translation="MNIINIEHISKLFGDKLIFDDASFGLQEGDKVGIVGINGTGKST
                     LLRMVAGEETPDSGQIIRQNNLKMAYLPQTPEFPKDATVLSYALSGDEEEWQVQSNLV
                     QLGITEYDAKLDTLSGGQRRKVALAKVLASDFDVLILDEPTNHLDNAMLSWLEDYLKN
                     YRGTVFMVTHDRYFLDKVSNRILEISHGKMYSYDSNYSRFLELKAEREEMELASERKR
                     QSILRMELEWAKRGCRARSTKQR"
     gene            complement(720..1157)
                     /locus_tag="PLN77_06940"
     CDS             complement(720..1157)
                     /locus_tag="PLN77_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRM44048.1"
                     /translation="MELETLRSDMIAAMKAKDKARKEAISSLISAAKKVAIDEGCRDD
                     IKPELVDRVILKELKTVKEQIDTCPASREDLKAEYQFRYDVISEYAPKQMSADEVKAY
                     IQEKFADVLATKNKGAIMKAVMADMKGKADGKVINQTVAELCK"
     gene            complement(1247..2398)
                     /locus_tag="PLN77_06945"
     CDS             complement(1247..2398)
                     /locus_tag="PLN77_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRM44049.1"
                     /translation="MEKSKVAVVACPDYEPARVKESLQKGLEAIGGLESLIGKEENIL
                     LKPNLVRSAKRERAVVTDPEVMDALITILQENGYENISCGDSCGLGTPEGIAKEAGLK
                     EVLEKHNVPLKDFLTSERVETADGKFLMIAKDALDCDALISVSKMKTHALERITGAVK
                     NQYGCISGVYKKLGHTQYPNAESFAHMLIELNQKVAPRLYICDGIVAMEGNGPTSGDP
                     VSMGVLLMSTDPIALDTVFCHLVNLDPSYVPTNLYGETLGLGTWHDDRIEIVTADGTI
                     SEEELKRKYGNPNFNVDRRKARKKGALDLLEILGVFQSRPYIIEEKCKKCGVCVESCP
                     VEGKAVRFDNGRRNPPVYDYKKCIRCFCCQEMCPHQAIQVKKHRLPWGK"
     gene            complement(2494..2850)
                     /gene="rplT"
                     /locus_tag="PLN77_06950"
     CDS             complement(2494..2850)
                     /gene="rplT"
                     /locus_tag="PLN77_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRM44050.1"
                     /translation="MARIKGGMNAKKKHNRVLKLAKGYRGARSKQYRVAKQSVMRALT
                     SSYAGRKQKKRQFRQLWIARINAAARMNGLSYSKFMYGLKLAGVELNRKVLADMAVND
                     AEGFATLAELAKSKVA"
     gene            complement(2900..3097)
                     /gene="rpmI"
                     /locus_tag="PLN77_06955"
     CDS             complement(2900..3097)
                     /gene="rpmI"
                     /locus_tag="PLN77_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRM44051.1"
                     /translation="MPKVKTSRAAAKRFKTTGTGKLVRNKAYKSHILTKKSQKRKRNL
                     RKQTTVDATNVKNMKKILPYM"
     gene            complement(3133..3627)
                     /gene="infC"
                     /locus_tag="PLN77_06960"
     CDS             complement(3133..3627)
                     /gene="infC"
                     /locus_tag="PLN77_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRM44052.1"
                     /translation="MINEQIRDREIRLIGQNGEQLGIMSARDAYLKAQEAGLDLVKIA
                     PTAKPPVCKIIDYGKYRYELARKEKEAKKKQKTIEIKEVRLSPNIDTNDLNTKVGAAR
                     KFITKGDKVKVTLRFRGREMAHMNSSKYILDEFAESLSDIATVDKAPKVEGRSMTMFL
                     TAKR"
     gene            complement(3936..4259)
                     /locus_tag="PLN77_06965"
     CDS             complement(3936..4259)
                     /locus_tag="PLN77_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HRM44053.1"
                     /translation="MPALSCSAMTCVYNQNELCSKGDIRVGGSGAKSSMETCCESFKE
                     RGEGTMSNSTGCGCTKIGVDCKAQNCTFNDNCKCEAGDIHIAGDSACSCQETECSSFR
                     CGCGK"
     gene            complement(4553..6490)
                     /gene="thrS"
                     /locus_tag="PLN77_06970"
     CDS             complement(4553..6490)
                     /gene="thrS"
                     /locus_tag="PLN77_06970"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRM44054.1"
                     /translation="MIITLKDGSTKEYSEPKSIIEIAADISEGLARMACAGEVNGEVE
                     DLRTVIDKDCNLSILTFNDEGGKAAFRHTTSHIMAQAIKRLYPETKLAIGPSIADGFY
                     YDVDRDTPLTSEDLEKIEAEMKKIVKEDLKLERFELPRAEAIAFMKEKEEPYKVELIE
                     DLPEDAVISFYRQGEFTDLCAGPHLMSTKPVKAFKLTSLAGAYWRGSEKNKMLQRIYG
                     TAFTKKAELEEYLHMIEEAKKRDHRKLGKELGLFMMSEDGPGFPFFLPKGMDLKNALM
                     DYWREIHRKAGYVEISTPIILNRRLWETSGHWDHYKENMYTTVIDDEDYAIKPMNCPG
                     GVLVYKSEPRSYRDLPLRLAEVGLVHRHEKSGQLHGLMRVRCFNQDDAHIFMTPEQIK
                     DEIKGVANLIDQVYKLFGFKYHVELSTRPEDSMGSDEDWEVATEGLRGALDDLGLDYV
                     VNEGDGAFYGPKIDFHLEDSIGRTWQCGTIQLDFQLPQRFELEYTGADGEKHRPIMIH
                     RVVYGSVERFIGILIEHFAGAFPTWLAPVQVKVLPISDKYADYGKKVLDALHEAGIRA
                     EMDTRSEKIGYKIREAQGQKIPYMLIAGAKEEEAGLVSVRSRFAGDEGQRSLEQFIAD
                     IKEEIASRVNRPVTVETKENK"
     gene            complement(6886..>7227)
                     /locus_tag="PLN77_06975"
     CDS             complement(6886..>7227)
                     /locus_tag="PLN77_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44055.1"
                     /translation="AYQDFEFHTYSGLPYSYHMKYGRSGTYTKELWINRREKSKSLVW
                     SSVRSAYQKVLELQQESERPVVERPKALGDIRGITYIYGIFYEFALLEMPEKAKEKIA
                     LQTAGQKSPEK"
BASE COUNT         1454 a         1802 c         1682 g         2289 t
ORIGIN      
        1 gcgctgtttt gtgcttcttg cgcggcagcc gcgtttcgcc cattcgagtt ccatgcgcag
       61 gatgctctgg cgtttccgtt cagaggcgag ttccatctcc tcgcgctcag ctttcagttc
      121 cagaaaacgg gaatagttgg aatcgtagct gtacattttt ccgtggctga tttcgaggat
      181 gcggttcgac actttatcca ggaaataacg gtcatgggtg accataaaga cggttccgcg
      241 gtaatttttc aggtaatcct cgagccagga gagcatcgca ttgtcgagat ggtttgtcgg
      301 ctcgtcgagg atcaggacgt caaaatcgga cgcaagcact tttgccaggg caaccttgcg
      361 gcgctggccg cccgagaggg tgtcgagttt tgcgtcgtat tcggtgatac cgagctgtac
      421 aaggttgctc tgcacctgcc attcctcctc atcaccggaa agggcgtagg acagcacggt
      481 ggcatccttt ggaaattccg gtgtctgcgg aagataggcc attttgaggt tgttctggcg
      541 gataatctgg ccggaatccg gggtctcctc tcctgctacc atgcgcagca gcgtggattt
      601 tccggttccg ttgattccga cgatgccgac cttgtcgccc tcctgcaggc cgaaggaagc
      661 atcgtcaaaa atcagtttat cgccgaacag tttgctgatg tgttcgatgt tgatgatatt
      721 catttgcaca gttctgcaac ggtctggttg atgacttttc cgtctgcttt acctttcata
      781 tctgccataa cggccttcat gatggcacct ttgttcttgg ttgccagaac gtctgcgaat
      841 ttctcctgga tgtaggcttt gacttcgtct gcactcatct gtttcggtgc atactcgctg
      901 atgacgtcat agcggaactg atactctgct tttaagtcct cacgggatgc cgggcaggta
      961 tcgatctgct cttttacggt cttcagctct tttaagatga cgcggtctac cagctccggt
     1021 ttgatgtcat cgcggcagcc ctcgtcgatg gctactttct ttgctgcgga gatcagggaa
     1081 gagatggcct cttttctcgc tttgtctttg gctttcattg ctgctatcat atcacttctt
     1141 aaagtttcta attccatggt gttgttcctc ctgtactaaa ttttgtccgg tttcaagtat
     1201 aacacttctc tttttgcttt tccagtcact ttgtaggata gctcccttat tttccccacg
     1261 gcagacggtg tttctttacc tggattgcct gatgcgggca catttcctga cagcagaaac
     1321 agcggatgca ctttttataa tcgtagaccg gcggatttct gcggccgtta tcaaagcgga
     1381 cggctttccc ttccaccggg cagctctcga cgcagacgcc gcatttttta catttttctt
     1441 ctatgatata ggggcggctc tggaagaccc cgaggatctc gagcaggtcg agggctcctt
     1501 ttttgcgggc tttgcggcgg tcgacgttga agtttggatt tccgtacttt cttttcagtt
     1561 cttcctcgga tatggtgcca tccgctgtca cgatttcgat gcggtcgtcg tgccacgttc
     1621 caaggccgag cgtttccccg tagagatttg tcggcacata cgacggatcg aggttcacca
     1681 gatggcagaa cacggtatcc agtgcgatcg gatcggtgga cataagaaga actcccatcg
     1741 aaaccgggtc gccggaagtc ggaccattgc cttccattgc tacgatgccg tcgcagatat
     1801 agaggcgcgg ggcgactttc tggttcagct cgatcagcat gtgcgcgaag ctctcagcgt
     1861 tcggatactg cgtgtggccg agttttttgt agacgccgct gatgcagccg tactggttct
     1921 tgacagctcc ggtgatgcgt tccagcgcgt gggttttcat tttgctgacg ctgatgaggg
     1981 cgtcgcagtc gagtgcatct ttcgcaatca tcagaaattt gccatcggcg gtttctacgc
     2041 gctcgctcgt aaggaaatcc ttcagcggaa cgttgtgttt ttccagcacc tctttcagcc
     2101 cggcttcctt tgcaatgccc tccggggtgc caaggccgca ggaatcgccg caggaaatat
     2161 tctcatagcc attttcctgc aaaatcgtga tcagggcatc catgacttcc gggtcggtca
     2221 cgacggcgcg ttcgcgtttt gcggagcgga cgaggttcgg ttttaataag atattctctt
     2281 cttttccgat cagagattcc aggccgccaa ttgcttccaa tcccttttgc aggctttcct
     2341 ttacgcgcgc cggctcatag tcgggacagg caaccactgc cactttgctt ttttccatat
     2401 tcacctcatg tttttctata tttcaaaaga agctgcgatc cccttaccgg ttctcacagc
     2461 ttcttcgtat attcacacag aatatgtttt tacttatgct actttgcttt ttgcaagctc
     2521 agccagtgtt gcaaatcctt ctgcatcgtt aactgccatg tcagccagaa ctttacggtt
     2581 cagctcaacg cctgccagtt tcagaccata cataaatttg ctgtaggaca gaccgttcat
     2641 tcttgctgct gcgttgatac gagcgatcca cagctgacgg aactgacgtt ttttctgttt
     2701 tcttcctgcg taggagctgg tcagtgcgcg cattacggac tgttttgcta ctctatactg
     2761 tttgcttctg gctcctctgt aacctttagc cagttttaat actctgttat gttttttctt
     2821 agcgttcatg ccgcctttaa ttcttgccat ttcttatttt cctccttgat ctgttgggcg
     2881 taactctacc ttgcaaaaat tacatgtaag gtaagatttt cttcatgttc tttacgtttg
     2941 ttgcatcaac agtagtctgt tttctcaggt ttctttttct cttctgagat ttcttggtta
     3001 agatatggct cttgtaagct ttatttctta ctaattttcc tgttcctgtt gttttaaaac
     3061 gctttgcagc tgctctgctt gtttttacct ttggcatttt aatttcctcc ttgatattga
     3121 ttcaaatgta ttttaacgtt tagccgtcaa aaacatggtc atgcttctgc cttccacttt
     3181 cggcgctttg tcaaccgttg caatatcgga cagactctct gcaaattcat cgaggatgta
     3241 tttgctgctg ttcatatgtg ccatctcacg gccgcggaaa cgaagtgtta cttttacttt
     3301 gtctcccttt gtaatgaact tgcgggctgc acctactttg gtattaagat cattggtgtc
     3361 aatgtttgga gaaaggcgaa cttccttgat ttcaattgtt ttctgttttt tctttgcttc
     3421 tttttccttg cgggccagtt catatctgta ctttccgtag tcgataattt tgcaaaccgg
     3481 cggtttcgct gtcggagcaa ttttcacaag atccagacct gcttcctgtg ctttcaggta
     3541 cgcatctctg gcagacataa ttccaagctg ttctccgttc tgaccgatca gacgaatttc
     3601 tctgtctctg atctgctcat taatcattaa ttcgctaatt gtagtgcacc tccatcatta
     3661 taaaagactg tgttcccgct tttgcggtgt aacgaaatca aactcggctc accggaccgg
     3721 atttgactga taacgcaaga aaagtggata gacagactat ccacttaaca ttgtacataa
     3781 atctctacac aaagagaact taagatcaat attggaccgt agtcgcgtgt ttgcgctagg
     3841 gtgagagtgg atactctact ttctttttct cagactattg cagtatagca ctctcccatc
     3901 ctgttgtcaa tactgatttt cgcatttttt cgtatttatt tcccgcagcc acagcggaag
     3961 gagctgcact cggtttcctg gcagctgcat gcgctgtctc cggcgatatg gatatctccg
     4021 gcttcgcatt tacagttgtc gttaaaggta cagttctgcg ccttgcagtc gacaccgatt
     4081 ttcgtacagc cgcagccggt ggagttggac atcgtgccct caccgcgctc cttgaagctc
     4141 tcgcagcacg tttccatgga gcttttggct ccgcttccgc ctacgcggat atctcctttg
     4201 gaacagagct cgttctggtt gtaaacacag gtcatagcgg aacaacttaa tgctggcata
     4261 ataaattacc tcctgaaatt tagttttctg cggtttcgcc cgcatagaaa ccattatttc
     4321 caagaggtaa ttttttatgc cttatttatt tgataatacc agtgtccaaa gtttggaaat
     4381 ccgacgcaag caaagcttgc acgaggattt ttaaattttg gacacgccaa aacatgagta
     4441 agtagccatt tttcgaagaa aaatgagcgg agtacgaatg ttttgaagat tgcgggagta
     4501 cgaagtacgg agcaatctgg tatcataggt gtcaattttt ttgacaccta ccttatttat
     4561 tttcttttgt ttcaacagta accgggcggt taacgcggct tgcgatttct tctttgatat
     4621 ctgcgataaa ctgctccagg cttctctggc cttcatcacc tgcaaaacgg cttctgacag
     4681 atacaagacc tgcctcttcc tcttttgctc cggcgatcag catgtatgga atcttctgtc
     4741 cctgtgcctc acggatctta tatccaatct tctcggaacg ggtatccatc tctgcgcgga
     4801 tgcctgcctc gtgaagggcg tccagaacct tcttgccgta atctgcgtat ttgtcggaga
     4861 ttggcagaac ctttacctgt accggtgcaa gccaggttgg gaatgcgcct gcaaagtgct
     4921 cgatcaggat accgatgaaa cgctcaacag aaccgtaaac aacacggtgg atcataatcg
     4981 gacgatgttt ctcgccgtct gctcctgtgt attccagttc gaatctctgc ggcagctgga
     5041 agtcaagctg gatggtaccg cactgccacg ttcttccgat ggagtcttcc agatggaagt
     5101 cgatcttcgg gccatagaat gcgccgtcgc cttcgttgac tacatagtca aggccaaggt
     5161 catcgagtgc gccgcggaga ccttcggttg ctacctccca gtcctcatcg cttcccatgc
     5221 tgtcttccgg acgggtggac agctctacgt gatatttgaa tccgaacagt ttgtaaacct
     5281 ggtcaatcag gtttgcaacg cctttgattt catctttgat ctgctccggt gtcatgaaga
     5341 tatgggcatc gtcctggttg aagcagcgta cacgcatcag gccgtgcagc tggccggatt
     5401 tctcatgacg atgtaccaga ccaacctctg ccagacgcag cggcagatca cggtaggaac
     5461 gaggctcgga tttgtaaacg agaacgccgc ccgggcagtt catcggtttg attgcataat
     5521 cttcatcgtc gatgacggtg gtatacatgt tttctttgta gtgatcccaa tgaccggagg
     5581 tctcccacag gcgacggttc aggatgatcg gtgtggagat ttctacgtat cctgcttttc
     5641 tgtggatttc acgccagtaa tccatcagtg catttttcag atccattcct tttggaagga
     5701 agaacgggaa tcccgggccg tcctcactca tcataaacag gccgagctct tttccgagtt
     5761 ttctgtggtc acgtttcttt gcttcctcga tcatgtggag gtactcttcg agctctgctt
     5821 tctttgtgaa tgcagttccg tagattctct gcagcatctt gtttttctcg ctgcctctcc
     5881 agtatgcacc tgcaaggctg gtcagtttga aggctttgac cggctttgtg ctcatcagat
     5941 gagggcctgc gcacaggtcc gtgaattcac cctggcgata gaagctgatg actgcgtctt
     6001 ccggcagatc ttcgatcagt tctactttat atggctcttc tttttctttc atgaaagcga
     6061 tggcttctgc tctcggcagc tcaaaacgct ccagtttcag atcctctttt acaattttct
     6121 tcatttctgc ttcgattttt tcaaggtctt cgctggtgag tggagtatct ctgtctacat
     6181 cgtagtagaa tccgtctgcg atggacggtc cgattgcaag cttggtctcc gggtacagtc
     6241 tcttgattgc ctgtgccatg atatgggatg tggtgtgacg gaatgctgct tttccgcctt
     6301 catcgttgaa ggtcagaatg gaaagattgc agtccttgtc gatgacggta cgaagatctt
     6361 ctacttcgcc gtttacttca cctgcacagg ccattcttgc aaggccttcg ctgatgtctg
     6421 ctgcgatctc aatgatggat ttcggttcgg agtactcttt ggtacttcca tctttcagtg
     6481 tgatgatcat ttgctttacc tctttctttg tatatttttc gcaagcgtgt ttcagaattt
     6541 ttatactttt tacagcaaca aaaaagtccc ttctgacacg cttatgctgt caaaagggac
     6601 gatgtatttt cattcaccgc ggttccaccc attttgtttt ttcaaaccac ttcatttgac
     6661 gataacggaa tcaccgggcc ggattggggc cgctcagagc tggtcttcga tcacgttcct
     6721 gttaaaaccc ttgcaccaca aaacggggtt tctctctgtc acattcactg tgatttactc
     6781 gtctcttcta cgctttgttt tttgtgttcg gtgctttttg gctcccgata gttttagatt
     6841 atatcaccca atgcgtggtt gtcaagacct gcatgggcga aaaatttatt tttccggcga
     6901 tttttgtcct gctgtctgca gagcaatttt ttcttttgct ttttccggca tttccagcag
     6961 ggcaaactcg taaaagatgc cgtaaatata cgtgattcca cggatatcgc ccagcgcttt
     7021 cgggcgctcg acaaccggtc tttcgctctc ctgctgtaat tccagcactt tctgatatgc
     7081 actccggacg gagctccaga caagactttt gcttttttct ctgcggttga tccagagctc
     7141 ttttgtatag gtgccgcttc tgccgtattt catatggtag gaatacggca gaccggaata
     7201 cgtatggaac tcaaaatcct gatacgc
//
LOCUS       DAOMUF010000105         7185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2752_length_7185_cov_3.079944, whole genome shotgun sequence.
ACCESSION   DAOMUF010000105 DAOMUF010000000
VERSION     DAOMUF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2752_length_7185_cov_3.079944"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLN77_06980"
     CDS             <1..1011
                     /locus_tag="PLN77_06980"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HRM44056.1"
                     /translation="RALLQNLSLAVSNTALELPSGRKLPLRLSGGVSWYPENSTDLST
                     LKKYADFAMYQVKKAEKGYITEFDLELFTKNAKETEMRRLFHKMLNEELFTYYFQPIV
                     SAADGSIYAYEALMRGNLPALTRPDQILQLAHEEECLHEIERLTMFLSAKSYATFLST
                     HQIRGDELLFVNSIASQYMNHDEAVAYVEQYSFLQDRIVIEITEEDSPDLTALLKKRE
                     MPGFSGVFALDDYGSGYSNEKTLLELAPAYIKLDLSIIRDIDSDPDKQQIVENIVSYA
                     HQRGMKIIAEGLETPAELLKVLELDVDLLQGYFLAKPSAVPKPVSAESVAVIERFRKH
                     LA"
     gene            1307..2281
                     /gene="bsh"
                     /locus_tag="PLN77_06985"
     CDS             1307..2281
                     /gene="bsh"
                     /locus_tag="PLN77_06985"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HRM44057.1"
                     /translation="MCTAATYQTRDFYMGRTLDYEFSYGDQITVTPRNYPFSFLHMPS
                     LSHHYAMIGMACVMDDYPLYYEAFNEKGLGIAGLNFVGNAVYFDPKPEKDNIAQFELI
                     PWILGTCASLAEAKESLSRINLISTPFKKNLPLAQLHWIIADASGAITVESTADGLHV
                     YDNPVGVLTNNPPFPMQMFSLNNYLHLSPKQPANTFSNQLDLSTYSRGMGGLGLPGDL
                     SSASRFARVAFVKQNSISGNSETESVSQFFHILNSVDQQRGCCEVADGKYEITLYTSC
                     CNATQGIYYYTTYDNHQISAVDMHRENLDGETLRCFAPVTGEQIHMQN"
     gene            complement(2342..2566)
                     /locus_tag="PLN77_06990"
     CDS             complement(2342..2566)
                     /locus_tag="PLN77_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44058.1"
                     /translation="MEKCSCHERLEAYYDLKVFLTIDPIEQIQRIEKRNGSEKAVEFQ
                     KKWIPLEELYFEKCRTRSRCDVCFTMCDEM"
     gene            2919..3869
                     /locus_tag="PLN77_06995"
     CDS             2919..3869
                     /locus_tag="PLN77_06995"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRM44059.1"
                     /translation="MQFYLAPMEGLTGYVYRNAYHKYFPAADRYFTPFITNKKMSSRE
                     RNDILPEHNEGMTVIPQILTNQAEDFLSLTKELREYGYDTVNLNLGCPSGTVVAKRRG
                     SGLLAWPNTLDAFLDEIFSSCDCRISIKTRLGTTDTDEWEDLLTVYDKYPLEELIIHP
                     RIQKDFYKFTPRMECYRTAYETSRCSLCYNGDIFSPDDFQNLCREFPDTEKVMLGRGV
                     LQNPWLIGMLRSADPAGGEASAPDKELLHAFCEDLCAGYARVISGDKNVLFKLKALWI
                     YLGMSFTNPQKYLKKIKKANRLAEYEEAVDALFREQELIL"
     gene            complement(4255..4531)
                     /locus_tag="PLN77_07000"
                     /pseudo
     CDS             complement(4255..4531)
                     /locus_tag="PLN77_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006597816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
     gene            complement(4558..5334)
                     /locus_tag="PLN77_07005"
     CDS             complement(4558..5334)
                     /locus_tag="PLN77_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRM44060.1"
                     /translation="MMKNLEVLEEIRKQGKNLIETKRDEFVKQNLLSDEFIQMMQKNK
                     KPFELLMSYYQCAIMEIETKFRVLNQEYSLSYDQNPIEGIKTRVKSYESIMRKIRVKD
                     IPVTLESIEENIRDIAGVRVICSFPSDIYKLADSFLKQDDITLIERKDYIQHPKESGY
                     RSLHLIVQVPIFLQDTKKLVYVEVQFRTIAMDFWASLEHKMRYKKNIPENHVKYLQDE
                     LYDCARQSAELDNRMQNIRNLIAEEAEEEDHCTFVTLPMR"
     gene            complement(5417..6118)
                     /locus_tag="PLN77_07010"
     CDS             complement(5417..6118)
                     /locus_tag="PLN77_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM44061.1"
                     /translation="MNKTLILVVEDDTPVHNLITTTLKTHDYKYISAKNGAGAIMEAS
                     THNPDIVLLDLGLPDIDGVEVIEKIRSWSNMPIIVISARSEDSDKIEALDAGADDYLT
                     KPFSVEELLARLRVTQRRLSQMQAENGVQSAVFENGALRIEYAAGCAYLDGEELHLTP
                     IEYKLLCLLSQNAGKVLTHTFITQKIWGSSWENDIASLRVFMATLRKKLEKNGEQTQY
                     IQTHIGVGYRMLKVE"
     gene            complement(6111..6344)
                     /locus_tag="PLN77_07015"
     CDS             complement(6111..6344)
                     /locus_tag="PLN77_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM44062.1"
                     /translation="MEDNGPGIPKESREHVFEMFYSGKNKVSDSRRSLGLGLALCRSI
                     VNAHGGEIALYDCDPQGCCFRFNLPLSEVNLNE"
     gene            complement(6292..>6684)
                     /locus_tag="PLN77_07020"
     CDS             complement(6292..>6684)
                     /locus_tag="PLN77_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44063.1"
                     /translation="ILTGTLASKLKENAKLSARDAFRTKILFNTSQLLQKAEDASEIF
                     DITATKLVKLLGRDLTAAPAGAQESRIVRGTLYSAQTGGKTEKIFDLREQEIIQWVLD
                     SRKRGEKCLDRGGGQRPRDSKRVQRACV"
     assembly_gap    6685..6726
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6727..7165)
                     /locus_tag="PLN77_07025"
     CDS             complement(<6727..7165)
                     /locus_tag="PLN77_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRM44064.1"
                     /translation="MARAFGANFTALYVQTAEPAEKTPEDQARLQKHIRLAEQSGAEI
                     VTTHSEDIALQIAEYARISEVTRIVIGKSMMDQGFFRKRRGWTERLTDLVPDLEIHII
                     PDSNIITIYLVGVMMTSILTSGYTCSVISSVISVVLFNYFLTEP"
BASE COUNT         1665 a         1868 c         1569 g         2041 t
ORIGIN      
        1 cgagcacttc tgcagaatct ttcccttgcg gtttcaaata cagccctgga gctgccaagt
       61 ggtcgtaaac tgccccttcg cctttccggc ggagtttcct ggtatccgga aaacagtaca
      121 gatctgtcta cgctgaaaaa atatgcggat ttcgcaatgt accaggtaaa gaaagcagaa
      181 aaagggtaca ttaccgaatt cgatctggag ctttttacaa aaaatgcaaa ggaaaccgag
      241 atgcgccgcc tgttccataa aatgttgaat gaagagctgt ttacgtatta cttccagcca
      301 attgtttccg ctgcggacgg cagtatctac gcctacgagg cgctgatgcg cggaaatctc
      361 ccggcactta ctcgtccgga tcagatcctg cagctggctc acgaggaaga gtgcctgcat
      421 gaaatagaac gtctgactat gtttctctct gcaaaatcct atgccacatt cctctccacc
      481 catcagatac gcggtgatga gcttctcttt gtcaattcga ttgcaagcca atacatgaat
      541 cacgacgagg cagtggctta cgtcgaacaa tacagctttc ttcaggatcg gattgtcatt
      601 gagatcacag aggaggattc accggatctt acagctctgc tgaaaaaacg tgagatgccg
      661 ggattttccg gtgtatttgc cctggatgac tacggcagcg gctacagcaa tgaaaaaacg
      721 cttctggagc ttgcaccggc gtatatcaag ctagatcttt ccatcatccg cgatatcgac
      781 tccgatccgg acaagcagca gatcgtagaa aatattgttt cctatgcaca tcaaagaggc
      841 atgaaaatca ttgccgaggg tctggaaact ccagctgaac ttttgaaggt actggaactg
      901 gatgttgatc tcctgcaggg ctattttctt gcaaaaccgt ccgctgttcc gaaacctgtc
      961 agtgcagaat ccgttgctgt catagaacgc ttccggaagc atcttgcgta attccacctc
     1021 ttcctgcagc gtcctttcaa atcacagcat ctatgtctga tctgctgctt cgaaacagat
     1081 accggtcagc gacccggagc gtatctgagc aatcatccct caaaaaaccc ccatagcaaa
     1141 ccctgcaaaa gcagggtttt gtctccactc tgaaggatcc tgcctgacag gatccttttt
     1201 tatacttttt tatacttttt tcatattttt ctcctgttcg ttcctccgct tctgtgctat
     1261 gataccttac agcaatgaca acccgtccat cgaaaggagt ttttctatgt gtacagcagc
     1321 aacctatcaa acacgcgact tttatatggg ccgcacactc gattatgaat tttcctatgg
     1381 cgaccagatc accgttacac ctcgcaatta tccgttttcc tttcttcata tgccgtccct
     1441 ttcccatcac tatgcaatga tcggcatggc gtgtgtgatg gatgattacc cgctctatta
     1501 tgaggcgttc aatgaaaagg ggcttggtat cgccggcctt aattttgtag gaaatgccgt
     1561 ttatttcgat ccgaaaccgg agaaagacaa tatcgcgcag ttcgagctga tcccgtggat
     1621 tctcggtaca tgtgcttcgc ttgcggaagc aaaagaatcc ctctcccgta tcaatctcat
     1681 cagcacacct tttaaaaaga atcttccgct cgcacagctt cactggatca tcgcagatgc
     1741 atcgggtgcc atcaccgtgg aatccacggc ggacggtctg catgtctacg acaatccagt
     1801 cggcgttctg acaaacaatc cgccgtttcc aatgcagatg ttctcactga acaattacct
     1861 gcatctgtca ccgaaacagc cggccaacac gttttccaac cagctcgatc tttctacgta
     1921 cagccgtggc atgggcggtc ttggactgcc gggcgacctc tcgtctgctt cccgctttgc
     1981 acgcgtggcg tttgtaaagc agaactcgat ctcgggaaac agcgaaacgg agagtgtcag
     2041 ccagtttttc catattttaa attctgttga ccagcagaga ggatgctgcg aggtggcgga
     2101 cggaaagtat gagatcacgc tctatacttc ctgctgcaat gcgacgcagg gaatttacta
     2161 ttatacgacg tatgacaatc atcagatttc tgcggttgat atgcaccgcg agaatcttga
     2221 tggcgaaacg cttcgctgct ttgcgccggt gacgggcgag cagattcata tgcagaatta
     2281 gaaaaatccg gtacagaaat ctttgaggac ccctcaaaaa agattctgta tcggattttt
     2341 tctacatctc atcacacatt gtaaaacaga cgtcgcaccg gcttctggtg cggcattttt
     2401 caaaatacag ctcctcgagc ggaatccatt tcttctgaaa ttccaccgcc ttttcactcc
     2461 cgttccgctt ttcaattctc tggatctgct cgatgggatc gatcgtgagg aagaccttca
     2521 gatcgtagta tgcttccagc cgttcatggc aggagcattt ttccaactgc cgggatctgg
     2581 ccgatgaaga acggcaggat caggccgatc gcaaagagct tgccggaaag ggttactttt
     2641 tttacttttt ccatcttgaa tgtcctccct tatgtttcct tctggaatca gtgtatcatt
     2701 caagtacact ttaaagtcaa gaccttttct gcatttttct ctatttttcc cactatttta
     2761 gtatattttg tattttccgt gcttctcggt gtctgactgc actttctgta ggaacttttt
     2821 ctgtcgtttt ccatggtttt gtggtacact tttggatatt gggaaatgca gtcattttct
     2881 gcagttccct gactattttt cgaaaggatt ttatcatcat gcagttttat ctcgccccga
     2941 tggaagggct gaccgggtat gtgtaccgga atgcttacca caaatatttt ccggcggcgg
     3001 accgttattt cacgccgttt attacaaata aaaaaatgag ttcccgcgaa cggaacgata
     3061 ttctcccgga gcacaacgaa ggaatgacgg tgattccgca gattctcact aatcaggcgg
     3121 aggattttct gtccctgaca aaggaactga gggagtacgg ttacgatacc gtcaatctga
     3181 acctcggatg cccgtccggc acggttgtcg ccaagcggcg cggctccggg ctgctcgcgt
     3241 ggccgaatac gctcgacgcg tttctcgatg agattttttc gtcgtgcgac tgccgcatct
     3301 cgatcaaaac gcggcttggc acaacggata ccgacgagtg ggaggatctg cttacggttt
     3361 acgacaaata tccgctcgag gaactgatca ttcatccgcg gattcagaaa gatttctata
     3421 aatttacgcc gcgcatggag tgctaccgga cagcgtatga gacgagccgc tgctcgctct
     3481 gctacaacgg tgacattttc tcacccgatg attttcagaa tctctgccgt gagtttccgg
     3541 acacggagaa ggtgatgctc ggacgcgggg tactgcagaa tccgtggctg attgggatgc
     3601 tgcgcagtgc agatccggca ggcggcgaag cctctgctcc cgataaagag ctgctgcacg
     3661 cgttctgtga ggatctctgc gccggttatg cgcgtgtgat ttccggggat aaaaatgtgc
     3721 tgttcaaatt aaaagcactg tggatttatc ttggaatgtc gtttaccaat ccgcagaaat
     3781 atctgaaaaa gatcaaaaag gcgaaccggc ttgccgagta tgaagaggcg gtcgacgcac
     3841 tgttccggga acaggagctg attctttaat ttcgaagtcc attagtatct tttggaaacc
     3901 atgccacttt caggtacgca cttgtctttt ctgcaaaatc cggtacgata gaaacaggat
     3961 acaacgactt atttctattt tgtttttggg aggattttta ccatgatcga tttacacaag
     4021 gaagacgaaa agctttttgc tcttgtcggc gcatgccagc gtgatgtcct tcttcgttcc
     4081 tccccactcg agaaactcct ctaacttttt cacactattt cctccttgta tacctatagg
     4141 ggtattggtt gtcaagagga gtttttcaaa atcagaaaaa caaaaagaac ttccgacgaa
     4201 gcatcttttt tctgctcctg ccggaagttc tcttttcatc ttatcttctt atttttattt
     4261 catatttgca aaccgctcca cagccttcgt aaactgtgca atcgtctgat ccgcatctcc
     4321 gtgctcgatg ccgtcgcgca cgcagtgctg gatatggccc tcgaggacaa cttgtcctac
     4381 tttatgaagt gccgatttta ccgcattgat ctgcgccagg atatcctcgc acggcacatc
     4441 ctcatccacc catacggtcg atcgcctgca cttgaccgat gatctttttc aggcggcggt
     4501 gaagattatc tgcatccata cactgtttca ttttgcatcc tccttgtttc aaatccgtca
     4561 tcgcatcggc agcgttacaa aggtgcagtg gtcttcctct tctgcttcct ctgcaatcag
     4621 attccggata ttctgcatac ggttgtccag ctccgcgctc tgtcttgcgc agtcgtagag
     4681 ttcatcctgc agatatttga catgattttc gggaatattt ttcttgtagc gcatcttgtg
     4741 ctccagactc gcccagaaat ccatcgcgat cgtacggaac tgtacttcca cgtagaccag
     4801 tttctttgta tcctgtaaaa agatcggcac ctgaacgatc agatgaagac tgcggtagcc
     4861 gctctccttc ggatgctgaa tatagtcctt ccgctcaatc agcgtaatgt catcctgttt
     4921 cagaaaactg tctgccagct tataaatatc gcttggaaaa gaacagatca cacgcacacc
     4981 ggcgatatcg cggatatttt cctcgataga ctccagtgtg accgggatgt ctttcacgcg
     5041 gattttccgc ataatgctct catacgattt gacccttgtt ttgattcctt caatcgggtt
     5101 ctggtcatag gaaagcgagt attcctgatt cagcacccgg aactttgtct cgatttccat
     5161 aatggcgcac tgataatacg acatcagaag ctcgaacggc tttttgttct tctgcatcat
     5221 ctgaataaac tcgtccgata aaagattctg ctttacaaac tcatcccgtt ttgtctcgat
     5281 cagatttttt ccctgttttc taatttcctc cagtacttcg aggtttttca tcatccgttt
     5341 tttcgtcctc ccaaaactgc gttctctgtc ctttatgcta tcattttagg cctgtaaagt
     5401 caaatttttt cttcttttac tctaccttca gcatccggta gccaaccccg atatgcgtct
     5461 ggatatactg cgtctgctct ccgttctttt ccagcttttt ccgaagcgtc gccatgaaaa
     5521 cacggaggga agcgatatcg ttctcccagc tgcttcccca gattttctgt gtgataaacg
     5581 tatgcgtcag caccttccct gcattctgcg acagcaggca gagcagcttg tattcgattg
     5641 gtgtcagatg aagttcctct ccgtcaagat atgcgcaccc cgctgcgtac tcgatccgca
     5701 gtgcgccgtt ttcaaagaca gccgactgga ctccattttc tgcctgcatc tgggacagac
     5761 ggcgctgtgt gactcgaagg cgggccagca gttcctccac agaaaacggt ttcgtcagat
     5821 aatcatccgc tcctgcgtcc aatgcctcga ttttatcgct gtcctcactt cttgcactga
     5881 ttacgataat cggcatattc gaccagcttc gaatcttttc aatcacctct acgccatcaa
     5941 tatccggcag gccaaggtcg agcagaacaa tgtcgggatt atgtgtggat gcctccataa
     6001 tggcacccgc accgttcttt gcagaaatat acttataatc gtgtgttttt aacgtcgtag
     6061 taatcaggtt gtgaaccggc gtatcatctt ccacaaccag aattaatgtc ttattcattc
     6121 aaattcacct cactgagcgg caggttaaac cgaaagcagc agccctgtgg atcgcagtca
     6181 taaagtgcta tctccccgcc gtgcgcattt acaatcgaac ggcagagtgc cagtccaagg
     6241 ccaaggcttc tgcggctgtc gcttacttta tttttcccgg aatagaacat ctcaaacaca
     6301 tgctctctgg actcttttgg aatcccgggg ccgttgtcct ccaccgcgat ccagacattt
     6361 ttctcctctt ttccggctgt cgagaaccca ctgaatgatt tcctgttccc ttaggtcaaa
     6421 gattttttcc gtctttcctc ctgtctgcgc gctgtaaagt gttccgcgga caattctgct
     6481 ctcctgtgcc cctgccggtg cggccgtcag atcccttccc agcagtttta caagctttgt
     6541 cgctgtaata tcaaaaatct ccgacgcatc ctctgccttc tgcagaagct ggctggtatt
     6601 gaaaagaatt ttcgtccgga acgcatcccg cgcagacagc tttgcattct cttttaattt
     6661 tgatgcaagc gtacctgtca gaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnngcgg ttctgtcagg aagtagttaa acagcactac actgatcaca gaactgatta
     6781 cgctgcaggt ataaccgctg gtcagaatgg acgtcatcat aacgccgacc agatatatgg
     6841 taatgatatt ggaatccgga atgatatgga tttccaaatc cggcaccaga tcggtcagcc
     6901 gttcggtcca tccccttctc tttcgaaaaa atccctgatc catcatgctt tttccaatga
     6961 caatccgcgt tacttcggaa attctcgcat attctgcaat ctgcagggca atgtcctcgc
     7021 tgtgcgtcgt tacaatctcc gccccgctct gttctgccag gcggatatgc ttctgcagcc
     7081 ttgcctgatc ctccggcgtt ttctctgccg gttccgcggt ctgcacatac agtgctgtaa
     7141 aattagcacc aaatgcccgg gccatctgtg ccgcagtccg gataa
//
LOCUS       DAOMUF010000106         7171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2762_length_7171_cov_4.463744, whole genome shotgun sequence.
ACCESSION   DAOMUF010000106 DAOMUF010000000
VERSION     DAOMUF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2762_length_7171_cov_4.463744"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="PLN77_07030"
     rRNA            <1..103
                     /locus_tag="PLN77_07030"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            188..259
                     /locus_tag="PLN77_07035"
     tRNA            188..259
                     /locus_tag="PLN77_07035"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:Asn,seq:gtt)
     gene            314..385
                     /locus_tag="PLN77_07040"
     tRNA            314..385
                     /locus_tag="PLN77_07040"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:347..349,aa:Glu,seq:ttc)
     gene            412..484
                     /locus_tag="PLN77_07045"
     tRNA            412..484
                     /locus_tag="PLN77_07045"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:445..447,aa:Thr,seq:tgt)
     gene            489..562
                     /locus_tag="PLN77_07050"
     tRNA            489..562
                     /locus_tag="PLN77_07050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:523..525,aa:Ile,seq:cat)
     gene            597..670
                     /locus_tag="PLN77_07055"
     tRNA            597..670
                     /locus_tag="PLN77_07055"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:631..633,aa:Asp,seq:gtc)
     gene            complement(791..1408)
                     /locus_tag="PLN77_07060"
     CDS             complement(791..1408)
                     /locus_tag="PLN77_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRM44065.1"
                     /translation="MKVLLINGSPHAKGSTYTALHEMEKVFTENGIEAEIVHIGNKDI
                     RGCVACGACSKLGKCAFSDMVNEVAPKFEEADGLVVGSPVYYASANATLIAFLDRLFY
                     STHFDKTMKVGASVVSARRGGLSATFDELNKYFTISGMPVASGQYWNSIHGNNADEAQ
                     QDLEGLQIMRTLARNMSFLMKSIQLGKESFGLPEKEPRVGTNFIR"
     gene            1568..4900
                     /locus_tag="PLN77_07065"
     CDS             1568..4900
                     /locus_tag="PLN77_07065"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRM44066.1"
                     /translation="MKIERILIEDQMAPIGLDEKKPAFSWLLTAEEGEQNLMQSACRI
                     VVRTGEDTVWDSGKMETGVSTGIGYEGEALQPCTKYEVKAEVWDNHGGKAEAESSFET
                     GLMDSLYAAWEGAEWIGAPHATVCAENRGVFTIESEFRMEGGKGEAGIVFGANDFRLN
                     DHTKNELGMEGENYIRYAVCLEDGDARLEIYRVGYAPEDTAEKPFAVTKLVNWKEKTQ
                     EILTPENADAFHKLTVEVDGNVAYAYVDGILVDAIEEHGFFGTKVSGRQLNPRGNNDV
                     LPYPRLNEIGFYAGKGTTGYFKNLTVRNVRKPSAEIVRETPEGRLDGGKSIFADQIPV
                     ENGYFKVEDSQITADPSHHSIPMLRSCISCQKEKKLVSARLYITARGIYDCRINGQEI
                     TEDLLRPGLTQYDHRMNYQTYDITGLMKPGRNGIGVTLASGWWSEAQTFVVKNFNYFG
                     DKESLLAKVVMKYEDGSREVFGTNTEDWKYFGEGPYLFSSYFAGEQYDARRAEVYETY
                     SLPEFDDSTWEKPVVVDTVMIEEFCTMPGFGRSWPAVHEQKPEYIGEYDAPVRIVDRR
                     TAKTRKELFPGVYLYDLEQEMAGVPRITLHEKAGTKIIIRYAEVLYPDLPEYAGKEGT
                     MMLENYRDATSTDVYICRGGEEVFQPKFTFHGYRYIEISGVEHAPELEEVESLQYSSV
                     TEFHGSLTSSYKLLNRFAENVSWSQKCNFINIPTDCPQRNERMGWAGDTHIFCHTALN
                     NSSLKKFYERNLQAMRDLQTPEGQYPEIAPVGGGFGGITYECASIFMAWELYEQYGDI
                     RTLEKFYPGMQKYMDYMKDKGLPGTKVNPAIGPLGDWLAPEETDLLLLWNAFYYKEAD
                     LMSRIAGALGRTEEQHQYEALAAKVKKFWNETFVLPDSGKTCNADGTLCDTQCSYAIA
                     LSYGVAEDRKRIGEHLIRKTRAIGHTVGTGFFGTGILNQMLTEQGAVEDAWKMMLQTA
                     FPSWLYPVTQGATTIWEHWDSYTKEKGFGGQNAMNSFNHYSLGSVLSWLYHTGLGIQR
                     DETKPGYQHILLKPQPGPLEFMKGSVDSPYGVISAGWERKGDKIEYQVTIPVNTTATL
                     FKNTEDPADTVELGSGTYTFML"
     gene            complement(5005..5379)
                     /locus_tag="PLN77_07070"
     CDS             complement(5005..5379)
                     /locus_tag="PLN77_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRM44067.1"
                     /translation="MKTNESAENYLETILVLSQKKPVVRGVDIAEELGFKKSSVSVAM
                     KNLREKEQIRVTPEGYIYLTDSGRAIAEMIYERHQLLSRGLMSLGVDEQTATEDACRI
                     EHVISQESFEAIKRHLHMTAEK"
     gene            5631..6809
                     /locus_tag="PLN77_07075"
     CDS             5631..6809
                     /locus_tag="PLN77_07075"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRM44068.1"
                     /translation="MEQVIKKRLQQVMDASVAKGETPGCLLMVIKDGEEQVYLESGYA
                     DIEAKKPVSRDNIFRLYSMSKPITATAMMILVERGIVDLADPVSKYLPGFRNPVVCTA
                     DGKIEKAEREILLEDIMNMTSGLTYGGTDETGRQTDALFQEVIHGLKEENGGTISTVE
                     FANRLGKVPLLYQPGQSWSYGTSADVVGAVIEVASGMRFGDFLKKEIFEPLGMNDTDF
                     WVPAEKQDRLAKVYDCREGQPSVRYLDNNLGIQNDMAYRPAYEAGGAGLASTIDDYAK
                     FTQMLLNGGTYNGVQILRPATVKFLGGHTLSAVQQAGFDNWIGLEGFSYGNLMRVLVD
                     PTRAVTLGSKGEFGWDGWLGCYMEVVPEQNMTFLMMTQKKDAGTYTLSRKIRNIIFSE
                     "
BASE COUNT         1989 a         1475 c         2031 g         1676 t
ORIGIN      
        1 aaagaagtaa gaccccttga agacgacgag gtagataggg cagaggtgga agtgccgtaa
       61 ggcatggagc tgactgttac taatcggtcg agggcttgac ctgaatggtt gtgttcacaa
      121 aaaatggaat gatagatgtg tagtgtatgc ggttttgaag gtatatcaaa tatcttcaat
      181 taaataatcc tcgatagctc aatggtagag cattcggctg ttaaccgaag ggttgttggt
      241 tcgagcccaa ctcggggagt gaatgcgacc tgtaagagtt tcttacaggt cagttctttc
      301 aaaaagtaaa tatggctccg tggtcaagcg gttaagacat cgccctttca cggcggtaac
      361 acgggttcga ttcccgtcgg agtcactgaa tataaaatat ttgttatgta tgccgatgtg
      421 gctcaattgg cagagcagct gatttgtaat cagcaggtta tcggttcgag tccgatcatc
      481 ggctctttgg acctttagct cagttggtta gagcaaccgg ctcataaccg gtcggtcccg
      541 ggttcgagtc cctgaaggtc cactttagtt ccatatttta tattctgtat atgtctggcc
      601 cggtggctca gctggttaga gcgccgccct gtcacggcgg aggtcgtcgg ttcgatcccg
      661 atccgggtcg ctttaaaaag tctgcggaag caggcttttt tcgtgtataa aatttcttcc
      721 agctataaaa gatgccgtat tccagacata gaaaaaagtc tgaaagacgg catcttgctt
      781 aacagataaa ttaacggata aagtttgtgc cgacgcgcgg ttccttttcc ggaagaccaa
      841 aggattcttt tccgagctgg atgctcttca tcagaaaact catattccgt gcaagggtac
      901 gcatgatctg caggccttcg agatcttgct gtgcctcgtc cgcattattt ccatggatac
      961 tgttccaata ctggccggaa gcaaccggca tgccggagat ggtgaagtat ttgttcagct
     1021 catcaaaggt agcggaaaga ccgccgcggc gtgcagagac aaccgaagcg ccgactttca
     1081 tggtcttgtc aaagtgtgtg ctgtagaaca gccggtcaag aaaagcaata agtgttgcat
     1141 ttgcagatgc ataataaacc ggacttccga caacaaggcc gtcagcctct tcaaatttcg
     1201 gagcaacctc atttaccata tcggaaaaag cacattttcc gagcttggaa caggcaccgc
     1261 aggcaacaca gccgcggata tctttatttc cgatatgtac gatttccgcc tcaataccgt
     1321 tttctgtaaa gactttttcc atttcgtgca gggcagtata agtgctgcct tttgcgtgag
     1381 gacttccgtt gattaataat actttcattt gatggtacct ctctttcgta atttccttta
     1441 ttataacacc ggaaaagatg caaataaaga cgcttttttg ggcaaaaact gatcttaaca
     1501 gaaatagctg ctggacggta taatacaatc agcaaagaag gcaaaacttg caatgggagg
     1561 ttaccaaatg aagatagaga gaattctgat tgaagatcag atggcaccga tcgggctgga
     1621 tgagaaaaaa ccggcatttt cctggctgct gacggcagaa gaaggagaac agaatctgat
     1681 gcagagcgca tgccggatcg ttgtgcgcac cggagaagat actgtgtggg acagcggaaa
     1741 aatggaaacc ggcgtatcaa cagggattgg ttatgaagga gaagcactgc agccctgtac
     1801 caaatatgag gtaaaagcag aggtgtggga taaccacggc ggaaaagcag aagcagaaag
     1861 ttccttcgag acaggcctta tggattcttt gtatgcagca tgggagggag ctgagtggat
     1921 cggcgcaccg catgcgacgg tatgcgcgga aaaccgcggt gtctttacga tcgagagtga
     1981 atttcggatg gaaggcggga agggagaagc gggtatcgtc tttggagcca atgacttccg
     2041 tctgaacgat catacgaaaa atgagcttgg catggaaggg gagaactata tccgttatgc
     2101 tgtgtgcctt gaggatggag atgcacgcct cgaaatttac cgcgtgggct atgcgccgga
     2161 ggatacagcg gaaaaaccgt ttgccgttac gaaactggtc aactggaaag agaaaacaca
     2221 ggaaatcctg acaccagaga atgccgatgc ctttcataag ctgaccgtag aggttgacgg
     2281 caatgtggca tacgcctatg tcgacggcat cctcgtggat gccattgaag agcatggatt
     2341 ttttggaacg aaagtatcag gccgccagct gaatccgaga ggaaataacg atgtgctgcc
     2401 gtatccgaga ctgaatgaga tcggatttta tgcaggaaaa gggacgacag gatattttaa
     2461 gaatctgacc gtaagaaacg taagaaaacc atccgcggaa attgttcgtg agacgccgga
     2521 aggacggctg gatgggggaa aaagtatctt tgcggatcag attccggtgg aaaacggata
     2581 ctttaaagta gaagacagcc agattacggc agatccgtcc catcattcaa ttccaatgct
     2641 gcggtcgtgt atttcctgcc agaaagagaa aaaactggta tcggcacgcc tttatattac
     2701 tgcgcgcggt atctacgact gccggatcaa cggacaggag atcacggaag atctgctgcg
     2761 tccgggtctg acacagtatg accacagaat gaactatcag acatacgata tcacagggct
     2821 gatgaaaccg ggccgaaacg gaatcggcgt gacgctggca tcgggctggt ggagcgaggc
     2881 acagaccttt gtggtgaaga acttcaatta ctttggagac aaggagagtc tgcttgccaa
     2941 ggttgtcatg aagtacgagg acggcagccg cgaggtcttt ggaacaaata ccgaggattg
     3001 gaagtatttc ggagaaggtc cttacctgtt ttccagctac tttgccggag agcagtacga
     3061 tgcacgccgg gcagaggttt acgaaactta ttctctgccg gagtttgatg acagcacctg
     3121 ggaaaagcca gttgttgtgg ataccgtcat gatcgaagag ttctgcacga tgccgggctt
     3181 tggacggtcc tggccggcgg ttcatgagca gaagccggaa tatatcggtg agtacgatgc
     3241 gccggtgcgg attgtggacc ggagaacggc aaagacgaga aaagagctgt tcccgggtgt
     3301 gtatctctac gatctggaac aggagatggc cggtgtaccg cggattacac tgcatgagaa
     3361 ggcgggcaca aagatcatca tccgctatgc ggaggtgctt tatccggatc tgccggagta
     3421 tgccggaaaa gaaggcacga tgatgctgga aaattaccgg gatgcgacga gtacggatgt
     3481 ttatatctgc cgcggcgggg aagaggtgtt ccagccgaag tttaccttcc acggctaccg
     3541 ctatattgag atcagcggtg tggagcatgc gccggagctg gaagaagtag agagccttca
     3601 gtattcttct gtgacggaat tccatggaag ccttacgagt agctataaac tgctgaaccg
     3661 gtttgctgag aatgtttcct ggtcacagaa atgcaacttt atcaacattc cgaccgactg
     3721 cccgcagaga aatgagcgca tgggctgggc gggcgatacg catattttct gccatacggc
     3781 gctcaacaac agcagcctga agaaattcta tgagcggaac ctgcaggcga tgcgtgatct
     3841 gcagacaccg gaaggccagt acccggagat tgcaccggtg ggcggcggat tcggcggcat
     3901 tacgtatgag tgtgcgagta tttttatggc gtgggaactg tatgagcagt atggggatat
     3961 ccgcacactg gagaaatttt atcctggcat gcagaaatat atggactata tgaaggataa
     4021 gggtctgccg ggaacgaagg taaatccggc gatcggaccg cttggcgact ggctggcacc
     4081 ggaagagacg gatctgctgc ttttatggaa tgcattctat tataaagaag ccgatctgat
     4141 gagccggatt gccggtgcgc tcggaagaac agaggagcag catcagtatg aggcgcttgc
     4201 tgcgaaggtg aagaaattct ggaatgagac atttgtactt ccggattccg gaaagacctg
     4261 caatgcggac ggaacgctgt gtgatacgca gtgctcgtat gcaattgcac tgtcttatgg
     4321 tgtggcagaa gacagaaaga gaataggaga gcatctgatc cgaaagacga gagcgatcgg
     4381 ccatacggtc ggaacgggat tctttggaac gggtattctg aaccagatgc tgaccgagca
     4441 gggcgctgtg gaggatgcct ggaagatgat gctgcagacg gcattcccgt cgtggctgta
     4501 tccggtgacc cagggagcga cgacgatctg ggagcactgg gattcctaca cgaaggaaaa
     4561 aggcttcggc ggacagaatg cgatgaactc gttcaatcat tattctctcg gaagcgttct
     4621 ttcctggctg tatcacacgg gacttggtat ccagagagac gagacaaaac cgggttatca
     4681 gcacattctg ctgaaaccgc agccgggccc gctcgagttt atgaaaggtt ccgtagacag
     4741 cccgtacggc gtgatttctg ccggatggga gcggaaaggt gacaaaatag agtatcaggt
     4801 tacgattccg gtcaatacaa cggcaaccct gtttaaaaac acggaggatc cggcggatac
     4861 cgtggaactg ggaagcggaa cgtatacttt tatgctgtaa aaagaaaacc cgaaaggctc
     4921 cggcaaagaa atgggagact ttcgggcgga aagagaaacg ctgtcggaga gaagagaact
     4981 ccggcagcgt tttctttgtc atgcttattt ttcggcagtc atgtgcagat gacgtttgat
     5041 cgcttcaaag ctttcctggc tgatgacatg ctcgatgcgg caggcatctt ccgtggcggt
     5101 ctgctcatca acaccaaggc tcatcaggcc gcgggagagg agctggtggc gctcatagat
     5161 catctctgcg atcgcgcgtc cgctgtcggt caggtagatg tagccttccg gcgttacgcg
     5221 gatctgctct ttttctctta agtttttcat ggcaacgctg acacttgatt ttttaaagcc
     5281 aagttcttct gcaatatcaa caccgcgtac aacgggtttt ttctgactta agactaaaat
     5341 ggtttccaaa taattttctg cggattcgtt cgttttcagc aagatgttca cctcgttttt
     5401 ctaaatagct gaagattcat aaatatatat acatatagaa tagcatattt ttcggaatag
     5461 ggcaatgatg gtttttgatg gtttttctta atttcagaaa agttgtaaga tttcagaaaa
     5521 aaaagacaat gaggatagaa gaagaaaagc aagataaaag atgacagggg gcaaaaagag
     5581 aggtaaaata ggaaaaaaca atgaaattta gatggaggtt agaacatacc atggaacagg
     5641 tgataaagaa aagactgcag caggtgatgg atgcgtctgt tgcaaaagga gagacaccgg
     5701 gctgtcttct gatggtgatc aaagacggag aggagcaggt ttatctggag agcggatatg
     5761 cggatatcga agcaaaaaag ccggtttccc gcgataatat tttccgtctc tactccatga
     5821 gcaagccgat taccgcgacg gccatgatga ttctggtaga gcgcggaatc gtcgatctgg
     5881 cggatccggt cagcaagtat ctgccgggct tccggaaccc ggttgtatgt acagcggatg
     5941 ggaagataga aaaagcggag cgggagatcc ttctggagga tattatgaat atgacatccg
     6001 ggctgacgta cggcggaacg gatgagacgg gacggcagac cgatgcactt tttcaggagg
     6061 tgatccacgg acttaaggaa gaaaacggcg gaacgatcag tacagtagaa tttgcaaacc
     6121 gtcttggaaa agtcccgctt ctgtatcagc cgggccagtc ctggagttat ggaacgtcgg
     6181 cggatgtcgt aggtgcggtg attgaggtgg caagcggcat gcgctttggg gattttctga
     6241 aaaaagaaat tttcgagccg ctcggcatga acgataccga tttctgggtt ccggcagaaa
     6301 agcaggatcg tctggcgaag gtgtatgact gcagggaggg acagccgtcc gtccgttatc
     6361 tggacaataa ccttggcatt cagaacgata tggcgtaccg cccggcatat gaggcaggcg
     6421 gagcaggcct tgcatctacc atcgacgatt atgcgaaatt tacgcagatg ctgctgaacg
     6481 gcggcaccta caacggtgta cagattctcc gtccggcgac ggtaaaattt ctgggaggcc
     6541 acacattaag cgcggtacag caggcaggct ttgataactg gatcggtctg gaaggatttt
     6601 cctacggaaa tctgatgcgt gttctggtag atccgacgcg tgcggtaacg ctgggaagca
     6661 agggtgagtt cggctgggac ggctggcttg gatgctacat ggaagtcgtg ccggagcaga
     6721 atatgacatt tctgatgatg acccagaaaa aagatgccgg aacgtacacc ctgtcccgga
     6781 agatccgaaa tattattttc agcgaataac ttttcatgag agtgattctc aagtaaggcc
     6841 ttccggcccg tcagatcaga cgaaaaaagt gccggaaaag aaacaactta acgaatcaga
     6901 cggaacatat gagcaaattg ctgtaaaact acgtcgaatt ttgtctaaaa cggatatttg
     6961 cagtgagaca gatgtatgct attttagtgt cagtcgaaaa tgaaggcgat ttcattacta
     7021 ctttcagaaa aaacagcaaa aaagggatat cgtgtaagtt tttgaccgat tttgaaggaa
     7081 ataaaagaaa gaattcaaaa tcatgaaaag aagactaaaa agcagaaagg atgcagaaaa
     7141 tatgatttcg ttaccgttaa agtttgatga a
//
LOCUS       DAOMUF010000107         7152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2775_length_7152_cov_4.556291, whole genome shotgun sequence.
ACCESSION   DAOMUF010000107 DAOMUF010000000
VERSION     DAOMUF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_7152_cov_4.556291"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /locus_tag="PLN77_07080"
     CDS             complement(<1..1694)
                     /locus_tag="PLN77_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HRM44069.1"
                     /translation="MQFGHFDDAKKEYVITSPKTPLPWINYLGSENFFSLISNTCGGY
                     SFYKDAKLLRLTRYRYNDTPLDQNGRYYYIKDGDTVWNPGWQPTQTPLDSYECHHGLG
                     YSRFVSSKNLVAAELTAFVPLADSCEVNRLMLTNNGTEEKTLALTSFVEFCFWNAVDD
                     MTNFQRNFSIGEVEIQGSEIYHKTEYRERRRHYAVYAVNCPIDGYDTDRDAFLGAYRG
                     NDRPETVLRGTAGNAVASGWGVIGSHHIDVTLKPGESRSFIFVLGYCENAADDKWEAP
                     GVINKKPAKEMLSHYQTDEQVDAALAELASYWEGLLAKYALSCADEKLGRMVNIWNQY
                     QCMVTFNMSRSASYFESGTGRGMGFRDSCQDLLGFVHLIPDRARERLLDIAATQFEDG
                     SAYHQYQPLTKKGNMDIGSGFNDDPLWLIAGCAAYIKETGDFSILDEQVDFDNDSTKA
                     QPLMEHLKRSFDFTVTHLGPHKLPLIGRADWNDCLNLNCFSAEPGEPFQTTGPSEGPV
                     AESIFIAAMFVKYGKEYAAICRRRGNEEEAVYAEKEVEKVYQAVLDAGWDGEWFLRA"
     gene            complement(1958..3148)
                     /locus_tag="PLN77_07085"
     CDS             complement(1958..3148)
                     /locus_tag="PLN77_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRM44070.1"
                     /translation="MSKGTEGHFDTLPIGRLGLIPLNSSAALGKKVDDYLVSWRKGRG
                     NEFAEKYVAAYEGYERDSYIIQSQVPRFGSGEAKGIINESVRGDDIYILLDVCNYSLT
                     YSLCGYTNHMSPDDHFQDLKRVIAAIGGKARRINVIMPFLYESRQHKRSGRESLDCAL
                     GLQELTAMGVENIITFDAHDPRVQNAIPLHGFETIQPAYQFIKNILRNAPDMKLDKDH
                     LMVISPDEGGMGRAIYMANNLGVDMGMFYKRRDYSTIIDGRNPIVAHEFLGADVKGKD
                     MIIIDDMISSGESMLEVAKLLKDREARNIYMCSTFGLFTSGLEKFDKAYEEGLFTKVL
                     TTNVVYQTPELLSREYYISCDLSKYIALIIDKLNHDASISGLLDPADRIQKVIKKFKN
                     HEKI"
     gene            complement(3145..4143)
                     /locus_tag="PLN77_07090"
     CDS             complement(3145..4143)
                     /locus_tag="PLN77_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM44071.1"
                     /translation="MALTNPQYDAIMRMYNERQLRHQREQQEHIRIAYEKIPRLAEID
                     AEIASLSVRKAKAMLNGDASMDLDLNAAIRDRSQERAALLSMHGYPADYLELTYDCPL
                     CRDTGYINGTQKCACFKKAAVDLLYRQSNVEELLKTENFSQFSLDYYSDSLTSTGTPL
                     TSREAAAAALEKSQAFVRNFGSSFENLFFYGDTGVGKTFLSHCIARELIEQSFCVIYF
                     TAFDLFDLFARYTFSSSEEAKDAHANIFDCDLLIIDDLGTELTNSFVSSQLFLCINER
                     ILRKKSTIISTNLPLDRFMETYSERTFSRISSNYTIIKLFGNDIRIQKKLLGGTKA"
     gene            complement(4165..5328)
                     /locus_tag="PLN77_07095"
     CDS             complement(4165..5328)
                     /locus_tag="PLN77_07095"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HRM44072.1"
                     /translation="MLTLNNDCLASVTAVPDLFLEQYMPAANGEFVKVYLYLLKLVKD
                     HQQDSTLSSMADFFSCTENDIVRALKYWEKQGLLSLSYTAGESVPTGIAFLPLVRKEE
                     SGILPTPESSISGPIAAAPAVSAVSVEIPQTEQTPAPSPQPAVSAPSASSPADDLSSS
                     GISGQSVEKKHAPGSREELQHVLYVAQTYFGHLLSPTEIECIMFFYDDLHFSADLLEY
                     LVEYCVSKGSTSIHYLKKVGLAWQESGIRDVASAKQETTTFNKHYYTILKAFGIRNRA
                     PVEKEAEMMDRWLNTYGFTIDLITEACQRTVSKIGQPSFQYAEGILSKWKEQGVRSMA
                     DVEQRDAEHQKRKKTASDQNAARKTAPAASSNRFNNFHQREYDYQKLEEQLLK"
     gene            complement(5865..>7152)
                     /gene="murC"
                     /locus_tag="PLN77_07100"
     CDS             complement(5865..>7152)
                     /gene="murC"
                     /locus_tag="PLN77_07100"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRM44073.1"
                     /translation="ISGSDAKESDLTRHLTSLGAQVFYGQKAENIIPGIDLVVYTAAI
                     HPDNPEFAAAKAAGIPMLTRAELLGQIMRNYDTPIAVSGTHGKTTTTSMLSHILMAAD
                     CDPTISVGGILPSIGGNIRVGHSETFLTEACEYTNSFLSFFPKIGIILNMDADHLDFF
                     KDIDDIRHSFRAFARLLPTDGMLIINADTPKYDYVTEGLGCKVVTYALENQAVADYTA
                     ANITYDEFDHPSFDCICRGELRGRYTLMVPGLHNVSNALAAIALADELQLPENAIHEG
                     FATFRGTDRRFQYKGTYHGAKVIDDYAHHPTEIRATMEAAENCAHRTLWCVFQPHTYT
                     RTKALLDDFAAALSLADHVVLADIYAARETDTLGISSKDIQERIVKLGTACEYFPSFA
                     EIENYLNENLAEGDMLITMGAGDVVKIGEELLASEK"
BASE COUNT         1694 a         1673 c         1972 g         1813 t
ORIGIN      
        1 gcacgcagga accattcgcc gtcccagcct gcatcaagga ctgcctggta aactttttcg
       61 acttcttttt ctgcgtaaac agcttcttcc tcattgcctc tgcggcggca gatggctgcg
      121 tactctttgc cgtatttcac gaacatagcg gcgatgaaaa tggactcagc aaccggtccc
      181 tcggaaggtc ctgtcgtctg gaacggttcg cccggctctg cagagaagca gttcaggttc
      241 agacagtcat tccagtctgc acggccgatg agcggcagtt tgtgcggtcc gagatgggtt
      301 acggtaaagt cgaaggaacg tttcaggtgc tccatcagag gctgcgcctt ggtggaatcg
      361 ttgtcgaaat caacctgctc gtcgagaata gagaaatctc cggtctcttt gatgtaggcg
      421 gcacatccgg caatcagcca gagcgggtca tcgttgaagc cgcttccgat atccatgttt
      481 ccttttttcg tcagtggctg gtactgatgg tatgcgcttc cgtcttcaaa ctgggtagcg
      541 gcaatatcga gcagacgctc tcttgcgcgg tccgggatca gatgaacgaa gccgagcaga
      601 tcctggcagg agtcacggaa gcccatgcca cggccggtgc cggactcaaa gtaggatgcg
      661 gaacgggaca tgttgaaggt aaccatacac tgatactggt tccagatgtt gaccatgcgt
      721 cccagttttt catctgcaca ggagagcgca tattttgcca gcaggccttc ccagtaggaa
      781 gcaagctctg caagtgctgc gtcaacctgc tcgtcggtct gatagtggga gagcatctct
      841 tttgccggtt ttttgttgat cacgccagga gcttcccatt tgtcatccgc tgcattctca
      901 cagtagccga gaacgaagat aaaggagcgg gactcgcctg gttttaaggt aacgtcgatg
      961 tggtgggaac cgatcacacc ccatccgctg gcaacggcgt tgcctgcagt gccgcgcaga
     1021 acggtctccg ggcggtcatt tccgcggtaa gcgccaagga aagcgtcgcg gtcggtatcg
     1081 tatccgtcga tcgggcagtt gactgcataa acagcgtaat gtctgcggcg ctcgcggtac
     1141 tcagttttgt ggtaaatctc agaaccctgg atctcaactt cgccgatgct gaagtttctc
     1201 tggaagttgg tcatatcgtc cacggcgttc cagaagcaga actctacaaa agaagtcaga
     1261 gccagtgttt tttcttcggt gccgttgttg gtcagcatga gacggtttac ttcgcaggaa
     1321 tctgccagcg gaacaaaagc ggtcagttca gcggcaacta agtttttgga agaaacaaag
     1381 cggctgtagc caaggccatg atggcactcg taggaatcga gcggcgtctg ggtcggctgc
     1441 cagcccgggt tccagacggt atcgccgtcc ttgatgtagt agtagcgtcc gttctgatcg
     1501 agcggcgtat cgttataacg gtaacgggtc agacgcagaa gttttgcgtc cttgtagaag
     1561 ctgtatccgc cgcaggtatt ggaaatcaga gaaaagaagt tctcagatcc cagatagttg
     1621 atccacggaa gcggagtctt cgggcttgtg atgacatact cttttttcgc atcatcaaaa
     1681 tgtccaaatt gcatgtgtga tacctccttc gtcgtacaat gtaatttcat tatacagccg
     1741 aaaaaagaaa aaactagcaa gaatttgcta aaaatataaa aaaacgccga aaccgttttc
     1801 gtgacagttt cggcgtttgt ggatattcac gaattgctcc gcaattctgc aaacattcgg
     1861 aatccgctgg cgctacgtcc tcatgtttgg cgggttccaa attcgaacgc tttatcgtgc
     1921 aagcacgaac agctttcgaa ctttgaaccc tggaatatca gatcttctcg tgattcttaa
     1981 atttcttaat aactttctga atacgatccg ccggatccag cagtccgctg atggaggcgt
     2041 cgtgattcag cttgtcgatg atgagagcga tatatttgct caggtcgcag ctgatgtagt
     2101 actcgcggga gagaagctcc ggtgtctggt atacgacgtt ggtggtcaga acttttgtga
     2161 agagtccttc ctcataagcc ttgtcgaatt tctccagtcc gctggtgaac agtccgaagg
     2221 tcgagcacat gtagatgttg cgtgcctcac gatcctttaa gagtttggca acttccagca
     2281 tactctcgcc ggaggagatc atatcgtcga tgatgatcat atccttacct ttgacatcag
     2341 caccgagaaa ctcatgggca acgatcgggt tgcgtccgtc aatgatggta gagtagtcgc
     2401 ggcgtttgta gaacataccc atgtcaacac cgaggttgtt tgccatgtag atagcacggc
     2461 ccataccgcc ctcatccggg ctgatgacca tcagatgatc cttgtcgagc ttcatatccg
     2521 gtgcattgcg caggatgttt ttgatgaact ggtaagccgg ctggatggtt tcaaatccat
     2581 gcagcggaat cgcgttctgg acacgcgggt cgtgagcgtc gaaggtgatg atgttttcta
     2641 cgcccatagc agtcagctcc tgcagaccaa gtgcgcagtc gagagactct cttccagatc
     2701 tcttgtgctg gcggctctca tacaggaatg gcataatgac attgattctg cgggcttttc
     2761 caccgatggc tgcgatgaca cgcttcagat cctggaagtg gtcgtccgga gacatgtggt
     2821 tggtgtagcc gcacagagaa taggtcaggc tgtagttgca gacatcgagc agaatgtaga
     2881 tatcgtcacc gcggacagat tcgttgatga taccctttgc ctcgcctgaa ccgaaacgcg
     2941 gaacctgaga ctggataatg tagctgtcac gctcatatcc ttcataggca gcgacatatt
     3001 tttccgcaaa ttcattgccg cgtccctttc tccagctgac cagataatcg tcgacctttt
     3061 ttccaagtgc ggcgctgctg ttcagcggga tcagtcccag tcttccgatt ggcagtgtgt
     3121 caaaatgtcc ttcagttccc ttactcatgc tttggttcct cccaaaagtt ttttctggat
     3181 ccgaatatcg tttccaaaaa gtttaatgat ggtgtagtta cttgaaattc gtgaaaaggt
     3241 acgttccgag tacgtctcca tgaagcggtc aaggggcagg ttcgtggaga tgatcgtcga
     3301 tttcttccgc aggatgcgtt cgttgatgca gagaaagagc tgggaagaga cgaagctgtt
     3361 ggtcagttcc gtacctaaat catcgatgat cagaagatcg cagtcaaaga tattggcgtg
     3421 agcgtctttt gcttcttctg aactggaaaa ggtgtagcgt gcaaacaggt caaacagatc
     3481 aaatgcagta aaataaatca cacagaacga ctgctcgatc agctcgcggg caatacagtg
     3541 cgaaagaaac gtttttccga caccggtgtc gccgtaaaag aagagatttt caaacgacga
     3601 gccgaaattg cggacgaacg cctggctttt ttcgagcgcg gcagcagcgg cctcgcggga
     3661 agtgagcggt gttccggtac tggtcaggct gtccgagtag taatccaggg agaactggct
     3721 gaaattttcg gttttcagaa gctcctccac gttggactgc cggtacagaa gatcgacggc
     3781 agcttttttg aagcaggcgc atttctgcgt gccgttgatg tagccggtgt cgcggcagag
     3841 cgggcagtca tacgtcagct cgagatagtc tgccgggtag ccgtgcatgg aaaggagcgc
     3901 cgcgcgttcc tgggaacggt cacggatggc agcattcagg tcgagatcca tggaagcgtc
     3961 tccgttcagc atcgcttttg ctttccgaac cgagagggat gcaatctcgg cgtcgatttc
     4021 cgcaaggcgc ggaatctttt cataagcaat gcggatgtgc tcctgctgtt ctctctggtg
     4081 gcggagctgc cgttcgttgt acatgcgcat aatcgcatcg tattgcgggt ttgtcagtgc
     4141 catagttgtt tcgtcctccg ggcgttattt cagaagctgt tcttctaact tctggtagtc
     4201 gtattctctc tgatggaaat tgttgaagcg gttacttgaa gcagcaggag cggtttttcg
     4261 tgccgcattc tgatcagaag cggttttctt ccttttctgg tgctccgcat cgcgctgttc
     4321 gacgtctgcc atggagcgga cgccctgttc cttccatttg gagagaatgc cttccgcgta
     4381 ctggaagctc ggctggccga tcttggaaac ggtgcgctga caggcttcgg tgataagatc
     4441 aatcgtaaaa ccgtaggtat tcagccagcg gtccatcatc tcggcttctt tttccaccgg
     4501 tgcgcggttg cggatgccga atgctttcaa aattgtgtag taatgtttgt tgaatgtcgt
     4561 ggtctcctgt ttggcagagg caacatcgcg gattccgctt tcctgccagg cgagtcctac
     4621 ctttttcagg tagtggatgc tggtgcttcc cttcgaaaca cagtattcca ccaggtattc
     4681 cagaagatcc gcggaaaagt ggagatcatc atagaaaaac atgatgcatt cgatttccgt
     4741 tggcgacaga agatgaccga aatacgtctg cgcaacgtaa agaacgtgct gcagttcttc
     4801 gcggctgccg ggagcgtgtt ttttttctac tgactgtccg gaaataccgg aactggaaag
     4861 atcatctgcc ggagaagagg cagatggggc agaaacggcc ggctgtggtg atggagccgg
     4921 ggtctgttcc gtctgcggga tttcaacgga tacagcagaa acagccggtg cagccgcaat
     4981 cgggccggaa atcgaggatt ccggtgttgg aaggattccg ctttcttcct tccggacaag
     5041 cggcagaaag gcgattccgg tcggaacgct ttccccagcg gtgtaggaga gcgagaggag
     5101 tccctgcttc tcccagtatt tcagcgcccg gacgatatcg ttttccgtgc aggaaaaaaa
     5161 atctgccatc gatgacagag tggaatcctg ctgatggtct ttgaccagct tcagcagata
     5221 aaggtacact tttacaaatt ctccgttggc tgcgggcata tattgttcca gaaaaagatc
     5281 cggcacagca gtcacactgg ccaggcagtc attgtttaat gtcagcatac tcaaaattac
     5341 accatcctgt tcttatcgtg cggcagattt ttacttttag cgatctgttt tttattatac
     5401 gtcttgcctt ctcatacaca accaagtata acacagtatt ttcgacaagt gaataggtgt
     5461 tttgcggaaa aaacattccg ggaaaaaggc acggtggatg aaaaaggact gtggacaatg
     5521 tggataatgt ggagaaatcc gttttgtgaa atattatcat ctaccaaaaa tgttgaaaaa
     5581 tggaggaaaa cagggaagta taggactttc tggtgatatc gttatgtaaa caaagttata
     5641 cacaggaaat ccacattgtg tggatcgtaa aaatgtgtat aaaattgtgg acaatgtgga
     5701 taactctgaa aacaggcgaa aatacaggaa aatagctgtg aataaaaatg tgcgtaacta
     5761 tttggcgaaa aggcagattg tgtggattcg gagcaaaaaa agaccctgtg gaaaaaagga
     5821 aaactccatt ttccacaggg agcaggtaaa gcgaaagaat gattttattt ttcggaagca
     5881 agaagctcct caccgatttt tacaacgtct ccggcaccca tggtgatcag catgtcgccc
     5941 tcggcaagat tttcgttcag atagttttcg atttccgcaa aggatgggaa atattcgcag
     6001 gcagtgccga gttttacaat gcgctcctgg atatctttgg aggagatgcc gagcgtatcg
     6061 gtttctctgg ctgcgtaaat atcggcgaga accacatggt ctgccaggga aagagcggca
     6121 gcgaaatcat cgagcagcgc tttcgttctg gtgtaggtat gcggctggaa gacgcaccac
     6181 agtgttctgt gcgcacagtt ttcagcggct tccatcgttg cacggatttc ggttggatga
     6241 tgcgcataat cgtcgatcac ctttgctccg tggtatgtac ccttatactg gaaacggcgg
     6301 tcggttccgc ggaaggtggc aaagccttcg tggatggcgt tttccggcag ctgcagttca
     6361 tcggccagtg caatggcagc cagggcgttg gaaacgttgt gaagtcccgg aaccatcagc
     6421 gtgtagcgac cgcgcagctc gccacggcag atgcagtcga aggacgggtg atcgaactca
     6481 tcgtaggtga tgttggcagc ggtataatca gcgacagcct gattctccag cgcgtaggtg
     6541 acaaccttac agccaagacc ttcggtcacg taatcgtatt tcggcgtatc ggcattgata
     6601 atcagcatgc cgtcggtcgg gagcagacgg gcaaacgcac ggaaagagtg gcggatgtcg
     6661 tcgatatcct tgaagaaatc gagatggtct gcatccatgt tgaggataat gccgattttc
     6721 ggaaagaagc ttaagaaact gttggtatac tcgcatgcct cggtcaggaa ggtttcggaa
     6781 tggccgacac ggatatttcc gccgatggac ggaagaatac cgccgacaga aatagtcgga
     6841 tcgcagtcag cggccatcag aatgtgggag agcatagaag ttgttgtcgt ctttccgtgt
     6901 gtaccggaaa cggcgatcgg tgtatcgtag ttgcgcatga tctgtccaag cagttcagca
     6961 cgggtcagca tcggaatgcc ggcagctttg gcggcggcaa attccgggtt gtccgggtgg
     7021 atcgctgcgg tgtaaacgac aaggtcgatg ccaggaatga tattttccgc cttctggccg
     7081 tagaaaacct gtgcgccaag agaagtcagg tggcgggtca ggtcactttc ctttgcatcg
     7141 gaaccggaaa tc
//
LOCUS       DAOMUF010000108         6917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2907_length_6917_cov_4.112504, whole genome shotgun sequence.
ACCESSION   DAOMUF010000108 DAOMUF010000000
VERSION     DAOMUF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2907_length_6917_cov_4.112504"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLN77_07105"
     CDS             complement(<1..190)
                     /locus_tag="PLN77_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRM44074.1"
                     /translation="MRKPELLVPASSLEVLKVAVIFGADAVYIGGEVFGLRAKAKNFS
                     MEDMKEGVEFAHQHGVKVY"
     gene            complement(187..852)
                     /locus_tag="PLN77_07110"
     CDS             complement(187..852)
                     /locus_tag="PLN77_07110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRM44075.1"
                     /translation="MIIDERMVTYINSLDTGHTPFLENLERTAKQDRVPIIRREMQSF
                     LKVFLKMKQPKRILEVGTAVGFSAVFMAQYGEPDAEITTIENYEKRIPIARENFEKSG
                     FADRITLIPGDAAEVLKTLDGPYDFIFMDAAKGQYIHFLPEVLRVLDKNGVLITDNVL
                     QDGDVIESRFAVERRNRTIHKRMREYLFELTHNEGLLTSVVPLGDGIAITMKSHEKRE
                     ETT"
     gene            complement(883..1296)
                     /locus_tag="PLN77_07115"
     CDS             complement(883..1296)
                     /locus_tag="PLN77_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="solute-binding protein"
                     /protein_id="HRM44076.1"
                     /translation="MSEKKRDAGYRAALTGAKGTVRLLIYVCVILVIILAAKTSYQFG
                     HDVFAEEPVASRGKGKEVTVQVRSGMEAKELGELLKDNGLIDESLLVFEVQYRLSGYY
                     GGIKDGSYVLNTAQTVDEMLEILAGVNTEGQPSAE"
     gene            complement(1323..1658)
                     /locus_tag="PLN77_07120"
     CDS             complement(1323..1658)
                     /locus_tag="PLN77_07120"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRM44077.1"
                     /translation="MDKVTECLDALIEEMRESRAYQNYLWMEEELEKDPDLKSRVDDY
                     RIRNYRLQQFENVDLYDAVDSLERDSYALRKSEKANAYLEAELEVCKMVQKVQDRISE
                     EIRIGVPEI"
     gene            complement(1664..3328)
                     /locus_tag="PLN77_07125"
     CDS             complement(1664..3328)
                     /locus_tag="PLN77_07125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRM44078.1"
                     /translation="MKKNNGSKLKIIPLGGLEQIGMNITVFEYEDSIIVVDCGLAFPE
                     DDMLGIDLVIPDVTYLKENIDKVKGFVITHGHEDHIGALPYILKEVNVPVYATKLTIG
                     LIENKLKEHNLLRTTKRKIIKHGQSINLGAFRIEFIKTNHSIADAAALAIYSPAGIIV
                     HTGDFKVDYTPVFGDVIDLQRFAEIGKKGVLALMCDSTNAERPGFTMSERTVGKTIDG
                     LFAEHQNSRIIIATFASNVDRVQQIINSAYKYGRKVVIEGRSMVNIISIAQELGYINI
                     PENTLIDVEQMKNYTGEQLVLITTGSQGESMAALSRMAAHLHKKISILPGDTVIFSSH
                     PIPGNEKAVSNVINELSAQGANVVFQDVHVSGHACQEEIKLIYSLVKPKYAIPVHGEY
                     RHLKAHAEVAQSLGIPKENIFLMYSGDVMELDEDGAAVTGKVHTGAIMVDGLGVGDVG
                     NIVLRDRQHLSEDGIMVVVLTLEKRTNQLLAGPDIVSRGFVYVRESEDLMGEAREVVE
                     EALDGCLSKNISDWGKIKSTIKDALGDYVWKKTKRRPMILPIIMEA"
     gene            complement(3507..3767)
                     /locus_tag="PLN77_07130"
     CDS             complement(3507..3767)
                     /locus_tag="PLN77_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRM44079.1"
                     /translation="MEKITFVTDDGGKEEFYVEEQTRINGVNYILVSDSKDDEANAYI
                     LKDISTDTDAEAEYVMVEDDTEFDAVSGVFASMLDDEADITY"
     gene            complement(3783..4226)
                     /gene="ruvX"
                     /locus_tag="PLN77_07135"
     CDS             complement(3783..4226)
                     /gene="ruvX"
                     /locus_tag="PLN77_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRM44080.1"
                     /translation="MRIMGLDYGSKTVGVAVSDPLGLTAQGIEIVRRKSENKLRQTLA
                     RIEEIAKEYGVEKIVLGFPKHMNNDIGERAEKSLEFKEMLERRTGLPVVMWDERLTTV
                     EADRTMMETGIRRENRKEYVDMIAAVFILQGYLDYLSHQKESQEN"
     gene            complement(4238..4501)
                     /locus_tag="PLN77_07140"
     CDS             complement(4238..4501)
                     /locus_tag="PLN77_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HRM44081.1"
                     /translation="MSGLSNTQYFKVKTEPEIQVKEVLETVYNAMVEKGYNPVNQIVG
                     YIMSGDPTYITSFKGARSLIMKVERDELVEELLKEYIKNESWK"
     gene            complement(4708..>6917)
                     /locus_tag="PLN77_07145"
     CDS             complement(4708..>6917)
                     /locus_tag="PLN77_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM44082.1"
                     /translation="IYVISRISGEGADRKAEPGDYYLSEQEEEELKAITECYDNTIVI
                     LNVGGVMDVSFLEKYNIAALVMLSQAGMEGGNALADVLSGAVTPSGKLTDTWGCRYED
                     YPSSATFSHNNGNIIEEKYYEGIYVGYRYFDSFEVEPRYPFGYGMSYTTFDVATENAA
                     WKLDAESKTITVTVKVTNTGSCAGKEVVQIYAACPFGKLKKERKRLVAFGKTALLQPG
                     ESETLHLKVPTVLLESYRTGKAVYCMEAGDYDFLVGTSSRDVTLAARLTLDKTVETEH
                     LTNICPLLDALKEIQPEEEKEERWRAEREQMWEEKKAEIPLLFLDEKGLIRDGKSAEE
                     MYKILKFGETNAAEAKECDANGCEFEAETTEAKEDAGNCKCGAEQPKWEERRRKAMEK
                     AAELAQKLTPEEKTALVCGRSSGSKEIIGAAAVTVPGAAGETTASLLEKYGVANVILA
                     DGPAGIRITSHYQKNPSDGSVYKMNMYQRLENRIFGTEFLHTDGEDYYQYCSAIPVGT
                     LLAQTFDTELLEEVGRMIGAELEEFGVTLWLAPGMNIHRNPLCGRNFEYYSEDPLVSG
                     KMAAALTRGVQSRYGVGTTIKHYACNNQEENRRGVSSIVSERALREIYLKGFEIAIKE
                     SQPKAIMTSYNKVNGVHTANSYDLCTTAARKEWGFAGIIMTDWTTTNADGGSSAAKCI
                     AAGNDLVMPGTDTDRREILDALSAENDQYLEEKDLTACAQRILEMIFTSNSYE"
BASE COUNT         1339 a         1836 c         1673 g         2069 t
ORIGIN      
        1 catacacttt cactccatgc tgatgtgcaa actccacacc ttctttcata tcttccatcg
       61 aaaagttctt cgccttcgcc cgaagtccga agacttctcc gccgatatat acggcatctg
      121 cgccgaagat cactgctact tttaatactt ccagactgct ggctggaacc agtaattccg
      181 gttttctcat gttgtttcct ctctcttctc atggcttttc atcgtgatcg cgattccatc
      241 tcccagcggc acgaccgatg tcagcagccc ttcattgtgt gtcagttcaa ataagtattc
      301 ccgcatccgt ttgtggatcg tgcggttccg gcgttccacg gcgaagcggg attcgatgac
      361 gtcgccgtcc tgcagcacgt tgtcggtgat cagtacgccg ttcttatcaa ggacacgcag
      421 gacttccggc aggaagtgga tgtactgtcc cttagcggcg tccataaaga taaaatcgta
      481 cgggccgtcc agggttttta agacttctgc agcgtctccc gggatcagcg tgatgcggtc
      541 ggcgaagccg gatttttcga agttctcccg tgcgatcggg atgcgttttt cgtaattttc
      601 tattgtggtg atctctgcgt ccggttcgcc gtactgtgcc atgaaaacag cagagaatcc
      661 tacggcagtt cctacctcca ggattctctt tggctgtttc atctttaaga atacttttaa
      721 aaagctctgc atttcacggc gaatgatcgg cacccggtcc tgtttggccg tccgctccag
      781 attttccaga aacggggtgt ggccggtgtc cagggaattg atgtaggtta ccattcgttc
      841 gtctattatc atgtaagatt cctcttgttt tgcaaacgtg cgttattccg cggacggctg
      901 tccttcggtg ttgacgcccg ccagaatttc cagcatttca tcgacggtct gtgcggtgtt
      961 cagcacgtag gaaccgtcct tgatgccgcc gtagtagccg gacaggcggt actggacttc
     1021 gaaaaccaga aggctttcgt cgatcagtcc gttgtctttc aggagttcgc caagttcttt
     1081 cgcctccatg ccggaacgca cctgtacggt gacttccttt ccttttcctc tggatgcgac
     1141 tggctcctcg gcaaacacgt catgtccaaa ctgatatgaa gttttcgctg ccagaatgat
     1201 cacaagaatc acgcagacgt aaatgagcag gcgcacggtt cctttcgcac ctgtcagcgc
     1261 tgcgcggtat ccggcatccc ttttcttttc ggacatgggc gctctccttt tgattttcgg
     1321 ttttagattt ccggtacgcc gatgcggatt tcttctgaaa tacggtcctg caccttctgt
     1381 accatcttac agacctcaag ctccgcctca agataagcgt ttgccttctc gcttttgcgg
     1441 agtgcgtagg aatcccgctc cagggaatct accgcatcgt aaaggtctac gttttcgaac
     1501 tgctgcagtc ggtagttgcg gattcgatag tcgtcgaccc ggctttttaa gtccgggtct
     1561 ttttcaagtt cctcttccat ccacaagtaa ttctgatatg ccctgctctc cctcatttct
     1621 tcgatgagcg catccagaca ttctgttact ttgtccatgg tatttatgcc tccatgatga
     1681 tcggaaggat catcggtctt ctctttgttt tcttccatac ataatcgccg agggcatctt
     1741 tgatcgtgga tttgatcttg ccccagtcgg agatattttt ggaaaggcag ccatccagtg
     1801 cctcttctac aacttctctt gcctcgccca tcaaatcctc ggattcgcgt acatagacga
     1861 aaccgcggga tacgatgtcc gggccggcca gaagctggtt cgttctcttt tcgagagtca
     1921 gtacaacgac catgattccg tcctcggaca gatgctggcg gtcacgcagg acgatatttc
     1981 ctacatcacc aacgccgagt ccgtcgacca tgatggctcc ggtgtggact ttgccagtta
     2041 ctgctgcgcc gtcctcatcg agttccatga catcaccgga atacatgagg aagatgttct
     2101 ctttcgggat gccgaggctc tgtgctactt ctgcgtgtgc ttttaagtgg cggtattcgc
     2161 cgtgtaccgg gatcgcgtat ttcggtttta ccagggagta gatcagcttg atttcctcct
     2221 ggcaggcatg tccggatacg tgaacgtcct ggaatacgac gttggcaccc tgtgcagaca
     2281 gttcgttgat gacgttcgaa acggcttttt catttcccgg gatcgggtgg gaactgaaga
     2341 tcacggtgtc gcccggcagg atcgagatct ttttgtgcag gtgtgccgcc atacgggaca
     2401 gtgctgccat ggactcgccc tggcttccgg tggtgatcag gacaagctgc tcgccggtgt
     2461 agtttttcat ctgctccaca tcgatcaggg tattttccgg gatattgatg tagcccagct
     2521 cctgcgcgat ggagatgatg ttgaccatac tgcggccttc gatgacaact ttacggccgt
     2581 atttataagc agagttgatg atctgctgca cacggtcgac gttcgatgcg aaggttgcga
     2641 tgatgattcg ggaattctga tgctcggcaa acaggccgtc gatcgtcttt cctacggtcc
     2701 gctcggacat ggtaaagcca ggccgctctg cattggtact gtcgcacatc agtgccagaa
     2761 cgcctttctt tccgatctct gcgaatctct gcaggtcgat gacatcacca aagactggtg
     2821 tgtagtcgac tttgaagtct ccggtgtgga cgatgatgcc agccggggag tagatggcca
     2881 gtgctgctgc atctgcaata ctgtggtttg ttttgataaa ctcaatgcgg aatgcgccca
     2941 ggttgatgga ctgtccgtgt ttgatgattt tccgttttgt ggtacggagc aggttgtgct
     3001 cctttaattt gttttcgatc aggccaatcg tcagtttggt ggcgtagacc ggcacgttca
     3061 cttcttttaa gatgtaaggc agggctccga tatggtcctc gtggccgtgg gtgatgacga
     3121 agcctttgac tttgtcgatg ttttctttca gatatgttac atccgggatg accaggtcga
     3181 taccgagcat gtcgtcttcc gggaatgcaa gtccgcagtc tacgacgatg atactgtcct
     3241 catactcgaa aacggtgatg ttcataccga tctgttccag accgccaagc ggtataattt
     3301 ttaatttact tccattattc tttttcaaat agcacctccg cgcatgtttt ccaaacgatt
     3361 ccgttaccgg tacggcttca cgcacgcatc ccctgagcgt cacttcaggc tgatggagca
     3421 tgtcccgcac ctgcaggatt tgccctttaa agcgctgtcc cggtcccgct tcgtctgcgt
     3481 cccggtatgg cttcaaaacg gctggttcaa taggtgatgt ctgcctcgtc atccaacatg
     3541 gaagcaaata cgccggaaac ggcatcgaat tcggtgtcgt cttctaccat aacatactct
     3601 gcctctgcgt cggtgtcggt ggagatatcc ttcagaatat aggcatttgc ctcgtcgtcc
     3661 tttgaatcgg ataccagaat atagttgact ccgttgattc tggtctgctc ctccacgtaa
     3721 aattcttctt tgccgccatc atctgttaca aatgttattt tttccataca atattccttt
     3781 gttcagtttt cctgtgattc cttctgatgg gaaagatagt ccagatatcc ctgcaggata
     3841 aaaacagctg caatcatatc tacgtactcc ttgcggtttt ccctgcggat tccggtttcc
     3901 atcatggtgc ggtctgcttc gaccgttgtc agccgctcgt cccacatgac aacgggcaga
     3961 cctgtccgtc tctccagcat ttctttgaac tcgagagatt tttcggcccg ctctccgatg
     4021 tcgttgttca tatgtttcgg gaagccgagt acgatttttt ccacgccgta ctcttttgct
     4081 atctcttcaa tccgggccag cgtctggcgc agcttattct ccgattttct gcggacaatt
     4141 tcgattccct gcgcagtaag acccagaggg tcgctgactg ccacccctac tgtttttgag
     4201 ccgtaatcta atcccattat tctcatagta attacgctca cttccaggat tcatttttga
     4261 tgtactcttt taacagttcc tctaccagct cgtcacgctc tactttcatg atcaggcttc
     4321 tggctccctt aaagcttgtg atgtacgtcg ggtcgcctga catgatatag cctacgatct
     4381 ggttgactgg attataccct ttttctacca tggcgttgta aacggtttcg agtacctctt
     4441 ttacctgaat ctccggctct gtttttactt taaaatattg tgtgttactt aatcctgaca
     4501 ttcttttcac gtcctcgtat ttattgatat catttatatt ctaatacatg ttatgggaaa
     4561 tttcaatcgt gaaattgccc aactatttga atgttacagt tcactgcatc tcagcgaact
     4621 gtaacattca aagcccgcat ggaaaatttt gctttgcgaa ggggcggcag ctatttctga
     4681 tgttatttta tccctgaaaa cgccgcatta ttcatatgaa ttggaagtaa aaatcatttc
     4741 caggattctc tgggcacagg cggtcaggtc tttttcctcg agatactggt cattttcggc
     4801 actcagcgca tccaggattt cgcggcggtc ggtgtcggtt cccggcatca caagatcgtt
     4861 tccggcagcg atgcattttg cggcagagga gcctccgtcg gcgtttgttg tggtccagtc
     4921 tgtcatgatg atgccggcaa agccccattc ctttcttgca gcggttgtac acagatcgta
     4981 gctgttggcg gtgtggacac cgtttacctt gttgtaggag gtcatgatcg cctttggctg
     5041 ggattctttg attgcaattt cgaagccctt cagatagatc tcacgcagag cgcgctcgga
     5101 gacgatgctc gatacgccgc ggcggttttc ttcctgattg ttgcaggcgt aatgtttgat
     5161 ggtcgttccg accccgtacc ggctctggac gcctcttgtg agggctgccg ccatttttcc
     5221 gctgacaagc ggatcctcag aatagtattc gaagttgcgt ccgcagagcg gattgcggtg
     5281 gatgttcatg cccggtgcaa gccagagggt cacgccgaac tcctccagct ctgcgccgat
     5341 catgcggcct acttcctcga gaagctctgt gtcgaatgtc tgcgccagaa gcgttccgac
     5401 cgggatcgca ctgcagtact gataataatc ttctccgtcg gtgtggagga attcggttcc
     5461 gaaaatacgg ttctcgagac gctgatacat gttcattttg taaacactgc cgtccgacgg
     5521 atttttctga taatgggagg tgatgcggat gcctgccggc ccgtcagcca gaatgacatt
     5581 ggcaacaccg tatttttcca gcaaggaggc ggttgtctcc ccggctgcac ccggtacggt
     5641 aacggcagcg gctccgatga tttctttgct tccgctgctg cggccgcaga caagagccgt
     5701 tttttcttcc ggtgtcagct tctgagcgag ttcggcggct ttttccattg ccttgcgccg
     5761 tctttcttcc catttcggct gttctgctcc acatttgcag tttccggcgt cctctttcgc
     5821 ttctgttgtt tctgcttcaa attcacagcc gttggcatcg cattcctttg cttctgctgc
     5881 atttgtttct ccgaatttca gaattttata catttcttcg gcactctttc catcgcggat
     5941 cagacctttt tcgtccagga acagcagcgg gatttctgct ttcttttctt cccacatctg
     6001 ctcccgttct gctctccagc gctcctcttt ttcctcttcc ggctgaattt cctttaatgc
     6061 gtcaagcagc gggcagatgt tggtcagatg ctcggtttcc acggttttat caagtgtcag
     6121 tctcgcagcc agggttacat cccggcttga ggttccgacc agaaaatcat agtctccggc
     6181 ctccatacag tatacggctt tgcctgtccg gtaggattcc agcaggacag ttggtacctt
     6241 aaggtgcagt gtctcggatt ctcccggctg cagcagtgct gtttttccaa aagcgaccag
     6301 acgttttctc tcttttttca gttttccaaa cgggcaggca gcgtaaatct gtacaacttc
     6361 ttttccggca caggagccgg tgtttgttac ttttacggtt actgtgattg tttttgactc
     6421 tgcatccagc ttccacgctg cattttctgt tgctacgtca aaggtcgtat aggacattcc
     6481 atatccgaac ggataacgcg gttccacctc aaagctgtcg aaataacgat agccgacgta
     6541 aatgccctca taatattttt cttcgatgat gtttccgtta ttatggctga acgtcgcgct
     6601 ggacggataa tcctcgtagc ggcagcccca ggtgtcggtc agctttccgc tcggggtgac
     6661 ggcaccggat aaaacatcgg ccagtgcatt tccgccctcc atgcctgcct gagagagcat
     6721 gaccagcgcg gcaatgttgt atttttccag gaaggagaca tccatcacac cgccgacgtt
     6781 taaaataaca atcgtgttgt catagcactc tgtgatggct ttcagttctt cctcctcctg
     6841 ctccgacagg taatagtctc ccggctctgc ctttcggtct gcgccctcgc cggaaatgcg
     6901 gctgattacg tagatgg
//
LOCUS       DAOMUF010000109         6890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_2927_length_6890_cov_4.034236, whole genome shotgun sequence.
ACCESSION   DAOMUF010000109 DAOMUF010000000
VERSION     DAOMUF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2927_length_6890_cov_4.034236"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2855)
                     /locus_tag="PLN77_07150"
     CDS             complement(<1..2855)
                     /locus_tag="PLN77_07150"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRM44083.1"
                     /translation="MKKEILKETREPGKSRKKKGQPKGRRNGFLEGLTGKDFTAAGLF
                     FFSLLLCRLFVLQYGVFGSSIDWISQHSTLADYFRKRFYATGNLFPDFAWNLGGGQNI
                     YHFAYYGLLSPTVLLSYLFPFIPMDLWVMGSSALLYAADAVLFYRWISKKGLHYGNCL
                     FTSLFFTCSTALLYHSYNQLMFVNYMPFLLLALLGVDRHFQKRKSGLLILSVLGMILT
                     SFYFSVGGLLALTAYAVTEYLKCGTENAAYEAEQENRKVSRNQKAGAEKKQRAGEKSA
                     GTGETLVGLRSFFGAAIRFALPVLAGILLSGILLIPSAAALFGGSGEGGRGTAAGGIA
                     GLFTDPAMWKPQFLILRLCYTPYGIGLSAFAGVALLGRAIGKSRLREKLLSFLLLVFF
                     CIPLFGYLLNGGLYSKDKVFIPFLPVLCAEVGLYVENQLVRKRECGKNRSTGNFKRKV
                     MTELRELLPYLIVLILMWNAKQETYFEPYWHLGILDVICVTACYLAWRWFPAKKRLRG
                     RELPFLPLVFSAVILAFAGKAINQEKHVMIPRKTYEALTDSKIAAAIREIRAEDPGWY
                     RMEQYGDGGQNLANVNRIWDIGQNVSTIYSSAYNAEYKKFRDETYGINEAFRNRMMQT
                     VSDDPLFWQLMGVKYILAETRPEGYELYRDYGDFQVYRAISAAPVAYVTDQVVSETEY
                     KSLPYPQNQEILETAAVIPDQEMRKTTAAITDQEKSAKSVDNFRAAADSDTKNGSVGP
                     LFHSCFQKVNLEIPETDQEDLRIQKTADGYEIETKKSVTVSATLPGAAEGATLLAVSF
                     EVENQKASHDMFIRINGQTNRLTGINHTYANGNTQFNYLVTMKEDGTVFIRMGKGHYI
                     LKNFETWTGMAQPLEKTEQLYSQAVTLIGGTTATYGGDGITGVVSAERDGYLITSIPY
                     DENFVLKVDGKETPLFRANMAFLGAKIPSGEHKIVLV"
     gene            complement(2852..3943)
                     /locus_tag="PLN77_07155"
     CDS             complement(2852..3943)
                     /locus_tag="PLN77_07155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM44084.1"
                     /translation="METISIIIPCYNEEEAIPVYYETMVRQMDEMEEQQKVQFELIFV
                     DDGSKDHSLFEMRRLAQKDMRCRYLSFSRNFGKEAAMYAGLQAAKGDYVTVMDVDLQD
                     PPSLLPKMYAMLQSGTCDCVAARRSSREGEGKIKSFLSNAFYGVINRLSRTEIVSGAR
                     DYRLMSRKMVDAVLEMSEYNRFSKGIFGWVGFHTEWLEFEHEDRCAGTSKWPLRKLLA
                     YSVDGITGFSVAPLSFASVMGVLFFLISMILIVFIIGRTLLFGDPVAGWPSMACILFL
                     VSGVQLFCTGIVGIYLSKTYLETKHRPIYLLKETSEETVKMHSVQPVKKQESAAKRRK
                     DAHTAEKKNVYFPEYADKRHASERGEIRA"
     gene            complement(4034..5782)
                     /locus_tag="PLN77_07160"
     CDS             complement(4034..5782)
                     /locus_tag="PLN77_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM44085.1"
                     /translation="MKKLTETEKRILLWMLTDLSMVLILCGKLLRKWASEFEWGMWRN
                     IEWGIGIGVTVVGVLGIAAALYLVWKFRKNTGNSKMTAFAGSEPEKAGKTAKSSEGAA
                     ADKSETDEIEKSRQMQFEARAAAEAEKEQLFLKKSEKVPNELLVLVGGFAFFGWLQPL
                     RHSYARYRIVAEWGTVIECVQTGLLFFGICGVFLLSAGILVCRYEKKQLREMSFFCRE
                     RKRYRDRTSLERQLAGQSKWPFRVALIAELLIVITMITGLINGMDGEVLSVILLGLTT
                     LILILFAWKTYRSRLNREMGLLMEQIHTMAEGSMESRIHEISEKSLLYPAFAELENIE
                     SAMQKSVEKQMQAERLKIDLITNVSHDLKTPLTSMVGYTDLLKKEDLTPAAQDYVEVI
                     SVKQEQLKEMIQSLFELSKSTSGTEKFQMEKMDMKRLIEQTLADMDDAIAQSNLSFRT
                     ALGDEPLPFLGDNGKMYRVIQNLVENILKYSLGGTRVYIDAGKENGEIFVTMKNISAY
                     EMNFEAEEMMERFVRGDESRTSEGHGLGLAIASSYTANMGGRMELAADGDLFKVILRF
                     PEAGEDSAAGETATKT"
     gene            complement(5760..6473)
                     /locus_tag="PLN77_07165"
     CDS             complement(5760..6473)
                     /locus_tag="PLN77_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM44086.1"
                     /translation="MSQYNILVVDDDREIVRSLGKLLELEGYRVKKAYNGMEALDILM
                     TEQIHLILLDVMMPKMNGLSALMKIREKNNIPIIMLSAKTEESDKVIGLSMGADDYVT
                     KPYNTAELMARVKSQLRRYFSLGAANGTTQSQDILKNGGLLLNRNTKELLVDGEPVRL
                     TATEYKIMELLMEHPGYVFSAEEIYSQVWQEDAYSVENTVMVHIRRIREKIEITPKNP
                     KYLKVVWGIGYKIEKIDGN"
BASE COUNT         1669 a         1853 c         1539 g         1829 t
ORIGIN      
        1 tacacaagaa caattttgtg ctcgcctgat gggatttttg cgccgagaaa tgccatgttt
       61 gcacggaaca gcggtgtttc tttcccatcc actttcagca caaaattttc atcgtatgga
      121 atgctggtga tgaggtatcc gtctctttcc gcggaaacca cgcctgtgat gccgtcgccg
      181 ccgtatgttg ctgttgttcc gccaatcagg gtaaccgcct gactgtacag ctgctccgtt
      241 ttttccaacg gctgtgccat accagtccat gtctcaaaat ttttcaggat ataatggcct
      301 tttcccatcc gaatgaatac ggttccatct tctttcatcg ttacaagata gttaaactgt
      361 gtattcccat tggcatacgt atggttgatg ccggtcaggc ggttggtctg gccgttgatg
      421 cggatgaaca tatcatgact cgctttctga ttctccacct cgaaagaaac ggcgagcagc
      481 gttgctccct ctgcggcacc cggcagcgtt gctgagacgg tcacggattt tttcgtctca
      541 atctcatatc cgtccgctgt cttctgaatg cgcagatctt cctgatcggt ctctggaatt
      601 tcaaggttta ctttctgaaa acaactgtga aacaggggtc ctacagaacc attctttgta
      661 tctgaatctg cagcggctct gaaattatcc acagatttcg ccgatttttc ctgatccgtg
      721 attgccgctg ttgtcttccg catttcctga tccgggatca cggctgccgt ctccagtatt
      781 tcctggttct gcggataagg taatgatttg tattccgtct cggaaaccac ctggtctgtc
      841 acgtaggcaa ccggcgctgc ggatatggca cgataaacct gaaagtcccc gtaatcccgg
      901 tacagttcat acccttccgg ccgtgtttct gccagaatgt acttcacgcc catgagctgc
      961 cagaacagcg gatcatcgga aaccgtctgc atcatgcggt tgcggaaggc ttcattaatc
     1021 ccatacgtct cgtcccggaa tttcttatat tctgcattgt atgccgagga ataaatcgtc
     1081 gaaacattct gtccaatatc ccatatccgg ttgacgttcg caaggttctg tccgccgtct
     1141 ccatactgct ccatacggta ccagccggga tcctccgcac ggatctcgcg gattgccgct
     1201 gcaatcttgc tgtccgtcag agcttcatac gttttgcgcg ggatcatcac atgcttttcc
     1261 tgattgattg cttttccggc aaatgcaaga atcacagcac taaagacgag cggcagaaac
     1321 ggaagctctc tgccccgcag ccgttttttt gccggaaacc accgccatgc cagatagcac
     1381 gcagtcacac aaatcacatc cagtatgccg agatgccagt atggttcaaa gtatgtctct
     1441 tgctttgcat tccacatcag aatcagaact atgaggtacg gaagcaattc tcgcagctct
     1501 gtcatgactt tgcgcttaaa attcccggtg cttcgatttt ttccacactc ccgcttccgg
     1561 acaagctggt tttccacata aagcccgact tctgcacaga ggaccggaag aaacgggatg
     1621 aatactttat ctttgctgta caggccgccg ttcagcagat agccgaacag gggaatacaa
     1681 aagaatacca gcaaaaggaa ggacaacagt ttctcacgca accggctctt tccgatcgct
     1741 ctgcccaaaa gcgccacccc cgcaaatgcg gagaggccaa tgccgtacgg cgtgtagcag
     1801 agacgcagga tcagaaactg cggtttccac atcgccggat cggtaaaaag tcctgcgatg
     1861 ccgcccgctg cggtgccgcg tccgccctcg ccgctgccgc cgaacaaggc ggcagccgat
     1921 gggatcagca gaataccaga aagaaggatt cctgcgagga ccggaagcgc aaagcggatt
     1981 gctgcgccaa aaaagctccg caaacccacc aaggtttcac ccgttccggc agatttttca
     2041 cctgccctct gctttttctc tgccccggcc ttttgattcc gcgacacttt tcgattctcc
     2101 tgttccgctt catacgcagc attttctgta ccgcatttca aatactccgt caccgcatac
     2161 gccgtgagcg caagcagccc gccgaccgaa aaataaaagc tggtcagaat catcccaaga
     2221 acggaaagaa tcagcagacc gctctttctc ttctgaaaat ggcggtccac gcccagcaga
     2281 gccagcagca gaaacggcat ataattgaca aacataagct gattatagga atgatataga
     2341 agtgcggtag aacacgtaaa gaaaagtgat gtaaaaagac agttgccgta atgcagaccc
     2401 tttttgctga tccatcgata aaaaagcact gcatccgccg catacagcag cgcggaggaa
     2461 cccatcaccc acaggtccat cgggatgaac gggaaaaggt aggataacag cacggtcgga
     2521 ctcagcagtc cgtagtaggc aaaatggtaa atattctgac ctcctcccag attccatgcg
     2581 aaatccggga agaggtttcc ggtcgcgtaa aaccgctttc ggaagtaatc ggcaagggtg
     2641 ctgtgctggc tgatccagtc aatcgaggag ccgaatacgc cgtattgcag gacgaacagc
     2701 cggcacaaaa gcaaagaaaa gaaaaacaga ccagccgccg taaaatcctt tccggtaaga
     2761 ccttccaaaa agccgttccg ccgtcctttc ggctgacctt tctttttccg tgatttcccc
     2821 ggctctcttg tttcctttaa gatttccttt ttcatgcccg gatttctcct ctttcagaag
     2881 catggcgttt gtcggcatac tctggaaaat acacattttt cttttctgcc gtatgggcat
     2941 cttttcttct ctttgcagca ctctcctgct ttttcaccgg ctgcacactg tgcatcttga
     3001 ccgtctcttc actcgtctcc tttaagagat agatcggacg gtgctttgtt tcaagatatg
     3061 tcttggaaag ataaatgcct acaatgcccg tgcagaaaag ctgtacaccg ctcaccagaa
     3121 aaaggatgca tgccatcgac ggccagcccg caacgggatc gccaaacagc agcgttcttc
     3181 cgatgatgaa cacgatcaga atcatagaaa tcaggaaaaa aagcacgccc atgacagatg
     3241 caaatgacag cggagctacg gaaaaacctg tgatgccgtc gaccgagtac gcgaggagct
     3301 ttcgaagcgg ccacttcgac gttccggcgc agcggtcctc gtgctcaaat tccagccatt
     3361 ctgtgtgaaa accgacccat ccgaaaatcc ctttggaaaa gcggttgtac tcactcattt
     3421 ccagcacggc atccaccatt ttgcggctca tcagacggta gtcccgtgca ccgcttacaa
     3481 tctctgttct ggaaaggcgg ttgatcacac cgtagaaggc atttgataag aaggatttga
     3541 tttttccctc gccctcccgc gagctccggc gtgccgccac acagtcacag gtaccgctct
     3601 gcagcatcgc gtacattttc ggaagaaggg acggcggatc ctgaaggtcg acatccatca
     3661 ccgttacgta atcgcctttg gccgcctgca gtccggcgta catggctgcc tcttttccga
     3721 aatttctcga aaacgagagg taacggcagc gcatatcctt ctgcgcaagc cggcgcatct
     3781 caaacaggct gtgatccttc gagccgtcgt caacaaagat cagctcgaac tgaaccttct
     3841 gctgttcttc catctcatcc atctgacgta ccatcgtctc gtagtaaacc ggaattgctt
     3901 cctcttcgtt gtagcatggt atgataatac tgattgtctc catttttttc ctcccgggga
     3961 ttacatactc tctcagaatc cttctgcaga tccttcctgc tgcaatcccg cttctgtctc
     4021 attcttatgc aagtcaggtt tttgtggctg tttctcccgc tgcagaatcc tcgcctgcct
     4081 ccgggaaacg caggatgact ttaaacagat cgccgtctgc cgcaagctcc atgcggccgc
     4141 ccatgttggc cgtgtagctt gatgcaatcg caaggccaag gccgtgcccc tcgctcgtcc
     4201 gtgattcgtc gccgcgcaca aagcgttcca tcatttcctc cgcttcaaaa ttcatctcat
     4261 aggcggaaat gtttttcatc gtcacgaaaa tctccccgtt ttccttccct gcgtcaatgt
     4321 aaactctcgt tccgcccagg ctgtatttca ggatattttc cacgagattc tgtatcacgc
     4381 ggtacatttt cccattatcc ccaaggaacg gcagtggctc atctccgagc gccgtgcgga
     4441 acgacagatt gctctgcgca atcgcatcgt ccatatcggc gagggtctgc tcgatcaacc
     4501 gtttcatatc cattttttcc atctggaatt tttcggttcc gcttgtcgat ttggagagct
     4561 cgaagaggct ttggatcatc tcctttagct gttcctgttt cacagaaatc acttcgacgt
     4621 aatcctgtgc cgccggtgtc agatcctctt ttttcagaag atccgtatag ccgaccatcg
     4681 aggtgagcgg tgttttcaga tcgtgcgata cattggtaat caggtcaatt ttgagacgct
     4741 ccgcctgcat ctgtttttct acgctcttct gcatagcgct ctcaatattt tccagctctg
     4801 caaacgccgg atacagcagg gatttttcgg atatttcatg gatccggctt tccatgctgc
     4861 cttctgccat cgtatggatc tgttccatca aaaggcccat ctcccggttc aggcgactgc
     4921 ggtatgtttt ccaggcaaac agaattaaaa tcagcgtggt cagcccaaga agaatgacag
     4981 acagtacctc gccatccatt ccgtttatca gtccggtgat catggtaatc acgattagaa
     5041 gttccgcaat cagtgccaca cggaacggcc atttactctg accggcaagc tgccgttcca
     5101 ggctggtccg atctctgtaa cgcttccttt ctctgcaaaa aaaggacatt tctctcagtt
     5161 gtttcttttc atagcgacag acaagaatgc cggcactgag aaggaaaacg ccacagattc
     5221 caaaaaacag aagacctgtc tgcacacact ctattacagt accccattcg gcaacaatgc
     5281 ggtatctggc atagctgtgc cggagcggct gaagccatcc gaaaaaagca aatcctccga
     5341 caagaaccag aagctcattt ggcacctttt cggatttttt cagaaacagc tgctctttct
     5401 ctgcttccgc agcggctctt gcctcaaact gcatctgccg ggatttttct atttcatctg
     5461 tctcggactt atctgcggct gccccttcgc tgctttttgc tgtttttccc gctttttccg
     5521 gttcgctgcc ggcaaaagca gtcattttcg agttcccggt attttttcgg aatttccata
     5581 ccagatacaa ggctgcggct atgcccaaaa cgccgactac cgtcactcca atgccgatac
     5641 cccattcgat gttgcgccac attccccatt cgaattcact ggcccatttc cgcagaagct
     5701 ttccgcacaa aatcagtacc atagaaagat cggtaagcat ccacagcagg atgcgttttt
     5761 cagtttccgt caattttttc aattttgtat ccaattcccc acaccacctt taaatatttt
     5821 ggatttttcg gcgtaatttc gattttctca cgaatgcgcc ggatatgaac catgacggta
     5881 ttttctacgc tgtaagcatc ctcctgccag acctggctgt agatttcttc ggctgagaat
     5941 acatagccgg gatgctccat cagaagctcc ataattttgt actcggttgc ggtcaggcga
     6001 acaggttcac cgtccaccag gagttctttg gtgtttcggt tcagcagcag accgccgttt
     6061 ttcaggatat cctggctttg tgtggttccg tttgcggcgc ctaaggagaa gtaacggcgg
     6121 agctgggatt taacacgggc catgagttcg gcggtattat acggctttgt cacgtaatca
     6181 tcggcgccca tcgaaagtcc gatgacttta tcgctctctt cggttttcgc ggaaagcatg
     6241 ataatcggga tgttgttttt ttctcttatt ttcatcaggg cggaaaggcc gttcattttg
     6301 ggcatcatga catcgagcag gatcagatgg atctgttcgg tcatgaggat gtcgagtgcc
     6361 tccatgccgt tgtaggcttt ttttaccctg tagccttcca gttctaatag tttgccgaga
     6421 gatcttacga tttcccggtc gtcgtctacg actaatatgt tgtattggct catctcgttt
     6481 ttcccctctt ggttctatgg tgtttatcat agcagagaaa acggagatta tttggataaa
     6541 aaactgacat ttttcttaaa atgtaatccc ccattacgtt ttcctgcttt ttttacaaaa
     6601 aaaacactga ttggtagagt tgtaagctgg tgtaaaaaat aaaaacaaac gcatataaat
     6661 tagtaaaaat gcctgccata gcaactgaaa atgtggaaaa agataaaaaa agtcaatata
     6721 agtggacaaa atacacaata tcaaacgggg attttatttg atatgatgta aatatcacaa
     6781 ataagcaaag aaaatgcgta ataaaaacgc acaaaacaac ggaaaggatg gggagtatgg
     6841 cgaaggaaaa aatgaaaaag aaaaaaggag agagaattac ccctgcaatc
//
LOCUS       DAOMUF010000110         6713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3048_length_6713_cov_3.012166, whole genome shotgun sequence.
ACCESSION   DAOMUF010000110 DAOMUF010000000
VERSION     DAOMUF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3048_length_6713_cov_3.012166"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLN77_07170"
     CDS             <1..356
                     /locus_tag="PLN77_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44087.1"
                     /translation="FGPKYMNEDFYDKVSLPEDQGDGVKLADDAINEKYVDTEKNCGF
                     PMVQYTDEDLSRITAIGTDIYKYVEAQYAHWVVDGGIDDEWDSYIDQLKAMGIDEFLQ
                     IQTDAYNAYKENLAK"
     gene            484..2529
                     /locus_tag="PLN77_07175"
     CDS             484..2529
                     /locus_tag="PLN77_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRM44088.1"
                     /translation="MFLEKEGKLIYSYDGETVCIEAWGKDALRVRATKNRSFTGRDWA
                     LEAKDAHAGEVEIFEDPSAKGGAFANMYEGTDTSYGKITNGKLHAVIDSGSVITFYHE
                     DGRVLLKEHFRRLRDEESMPLNIMGREYSNGVGDNFRIVARFVARKGEKIFGMGQYQQ
                     HELDLKGCMLELAQRNSQVSVPFYLSSIGYGFLWNNPGVGQVMFANNGTEWVTESGKE
                     IDYLVIAGDTPAEIEENYMTLTGKPPMMPEYGMGFWQCKLRYWNQEQLLSVARKYKEL
                     GVPLDVIVVDFFHWTKQGEFKFDPKYWPDVPGMCRELKEMGIQVVVSVWPTVDIYSEN
                     FEEMKEKGYLVRTEHGVPLTMLCGGNEVFFDATNPDARTYVWEKIKKNYYDQGAKLFW
                     LDVAEPEYSVYDFKNYRYQLGSVQEVGNIYPKYYLKAFYDGMTAEGDTMPISLIRSAW
                     AGSAKYGALVWSGDIVSTFECFRRQVQAGLNMAVAGIPWWTTDIGGFHGARTDDPDFH
                     RLYIRWFEYGCFCPVMRLHGNRNPQEGYGAEQIGSGSDNEIWSFGDEAYEISKKYIFL
                     RERLRDYIRVQMKKAHEDGTPVMRPVFYDFPTDPESWNVEDAYLFGPDLYVAPVMEDH
                     VMEREVYLPAGTAWFNAWTGEKYEGGQKVTVAAPMDIIPVFVKEGADAEKLLNIFSE"
     gene            2767..4470
                     /locus_tag="PLN77_07180"
     CDS             2767..4470
                     /locus_tag="PLN77_07180"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM44089.1"
                     /translation="MNLWQKGKRRMLRSVRIRSLKDTSLRLGTPVFLVLMGFVVCLLW
                     GVGENRKLADKYIADTAKLYVDQINRDMSQINSELIYLLDSDSNIKEIPDQITSTDAQ
                     YYEMEQTLRERNRVLKIRYHEIQTFFVYGQKANVLITDSGTMFTESKGMTTLNQMLMS
                     YLQIMTSKDSISTQWTVITFGDDDYIVGWYAKNKKVIGCVINLNLIFRTIRAKTEEYD
                     VIPFMVDARGRVMTQADTSEKYKNEIIDFSKAGKKKKNATVYSYQLGTVGKINFMVLP
                     GGGILENVLIMQIAFVVLIAVLLIVCALEITAYYHRILEPLEKFGQKLEELEKEQSLN
                     DDGSNNLLELESVSGKFKELLRKIQGLKIAIYEKELAEQKAELEYTQEQIKPHFFLNC
                     LSLIHGIADKNNEGEIVEITTVLSDYMRYIFQDSKKQRDVEEELEHIEAYIRIQKLRY
                     GEEAFSFEATTDGDIGEWKVPSLLLQTLVENSVVHGVSLDRKSEISLYLTNETYEDGE
                     YLYICISDTGNGFSKEALDAIENDTPIIYHGRHHVGISNIKRRLALMYGVVEVRLPKT
                     E"
     gene            complement(4614..6566)
                     /locus_tag="PLN77_07185"
     CDS             complement(4614..6566)
                     /locus_tag="PLN77_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRM44090.1"
                     /translation="MVKKFVYGTPFETEAVVKEIPSSEGNPDYGTFSTENGFSFTTKL
                     ADDDMVFGLGEANRGINKRGFLYISDCADDPNHVESKTSLYAAHNFIIISGKTHVGFF
                     FDYPGTLRFDIGYTTSDTMTVSCENADLYVYMITGDSDLDIVKQFRGIIGRSYIAPKF
                     AFGYGQSRWGYKTEDDFRTVVKKYRENHVPLDMVYMDIDYMQDYKDFTVNEERFPDFE
                     GFVQDMKKEKIHLVPIIDAGVKVEEGYDIYEEGCEKGYFCRREDGSYFEATVWPGWTH
                     FPDVLNADARKWFGDKYDVLVSKGIDAFWNDMNEPSIFYSQEGLADFKETAKKYVEGD
                     PEVPHYMVGGKLQALANNHEDYKRFYHNVNGEQVRHDKVHNLFGYNMTRSAGEAFERI
                     SPDKRILMFSRSSYIGMHRYGGIWTGDNCSWWSHILLNLKMMPSLNMCGFLYTGADLG
                     GFGANTTRDLLLRWLALGVFTPLMRNHAALGTREQEPYQFEHIEDFRNVIGVRYRLVP
                     YLYSEYMKAALNDDMYFKPLAFVYPDDKLARNTEDQLMIGNEIMIAPVYTQNAIGRYV
                     YLPEEMMFVKFLGNGNMFQEVLPKGIHYVEVALNEVPLFIRKGCAIPVADFAESVPDI
                     KEESIRMVGYEGASYERYTDDGVSRI"
BASE COUNT         1837 a         1461 c         1800 g         1615 t
ORIGIN      
        1 acttcggacc aaaatacatg aacgaggatt tctatgacaa agtttctctt ccggaggatc
       61 agggcgacgg tgtcaaactg gccgatgatg cgatcaatga gaaatacgtt gatactgaga
      121 aaaactgtgg attcccgatg gttcagtata ccgacgaaga cctttccaga atcacggcaa
      181 tcggaaccga tatctataaa tatgtagaag cacagtatgc acactgggta gtagacggcg
      241 gcatcgatga tgaatgggat tcctacatcg atcagctgaa agctatgggt attgacgagt
      301 tcctccagat tcagacagat gcttacaatg catacaaaga aaatctggca aaataaacag
      361 aaaataacat tacctgtaag aagcaggcgt tgatccggcg gatcaatgtc tgaggtgcaa
      421 aacagcagga gaaacggcag gccagaaatg acctgccgtt tttgacaaaa ggaggatttt
      481 tacatgtttt tggaaaaaga gggtaagtta atttattctt acgatggcga gaccgtctgc
      541 atcgaagcgt ggggaaaaga tgcactgcgt gtacgcgcga caaaaaatcg aagctttacc
      601 ggccgcgact gggcgctgga agcaaaggac gcgcatgcgg gagaggttga aatctttgag
      661 gatccatcag caaaaggcgg agcctttgcc aatatgtacg agggaacgga tacttcctac
      721 ggaaaaatca caaatggaaa gcttcatgca gtgatcgatt ccggcagcgt cattacgttc
      781 tatcatgagg atggaagagt tctgttaaag gagcatttcc gccgtctgcg cgatgaggaa
      841 agcatgccgc tcaacatcat gggacgcgag tacagcaacg gcgtcgggga taatttccgt
      901 attgtagcgc ggtttgtagc aagaaaaggt gagaaaatct tcggaatggg acagtatcag
      961 cagcatgagc tggatctgaa gggctgtatg ctggagctgg cacagagaaa ctcacaggtc
     1021 agtgttccgt tctatctttc cagtatcggc tacggcttct tatggaacaa tccgggcgtc
     1081 ggacaggtga tgtttgcgaa taatggaacg gaatgggtaa cggaatccgg gaaagaaatc
     1141 gattatcttg tgattgcagg tgatacgcct gccgagatcg aggagaatta tatgacgctg
     1201 accggaaaac cgccgatgat gccggaatat ggcatgggct tctggcagtg taagctccgc
     1261 tactggaatc aggaacagct tctgtctgta gcaagaaaat acaaagagct tggcgtaccg
     1321 cttgatgtga ttgtcgtcga tttcttccac tggacgaagc agggcgagtt caaatttgat
     1381 ccgaaatact ggccggatgt gccgggcatg tgccgcgaat taaaggagat gggaattcag
     1441 gttgttgtgt cggtatggcc gaccgttgac atttattccg aaaacttcga ggagatgaaa
     1501 gagaagggct atctggttcg cacggagcac ggtgtaccgc tgacgatgct ctgcggcgga
     1561 aatgaagttt tcttcgatgc gactaacccg gatgcaagaa catatgtctg ggagaagatc
     1621 aagaaaaatt attatgatca gggtgccaag ctcttctggc tggatgtggc agagccggaa
     1681 tacagcgttt atgatttcaa gaactaccgt tatcagctcg gaagcgtaca ggaggtcgga
     1741 aatatttatc cgaaatatta tctgaaagcg ttctatgacg gcatgacggc agaaggcgat
     1801 accatgccga tcagcctgat ccgttctgcc tgggcaggca gcgcgaaata tggtgcactc
     1861 gtatggtcag gcgatattgt aagtaccttc gagtgcttcc ggcgccaggt acaggcaggt
     1921 cttaacatgg cggttgccgg tattccgtgg tggaccactg atatcggcgg cttccacggc
     1981 gcaagaaccg atgacccgga tttccatcgt ctgtacatcc gctggtttga gtacggatgc
     2041 ttctgccctg tcatgcgtct gcacggaaac cgcaacccac aggagggcta cggtgcagaa
     2101 cagatcggat ccggcagtga caatgagatc tggagctttg gtgatgaggc atatgaaatt
     2161 tccaagaaat atattttcct gcgtgagcgc ctgagagatt atatccgggt acagatgaag
     2221 aaagcacatg aggacggaac cccggtgatg cggcctgttt tctatgattt cccgacagat
     2281 ccggaaagct ggaatgtgga ggacgcttat ctgttcggac cggatctcta tgttgcacct
     2341 gtgatggagg atcacgtaat ggagcgcgag gtttatcttc cggccggaac ggcatggttt
     2401 aacgcatgga ccggagagaa gtatgaggga ggacagaagg taacggttgc tgctccgatg
     2461 gatattattc cggtattcgt aaaagaagga gcggatgcag aaaagctgct gaatattttc
     2521 tctgaataag agaatgttac cgctcaccgg taccaggcga acggtaacaa agaatcctgt
     2581 tctcaccatg taaagcacgg actctgcatg gacaaagccc aaaaatgtga tataatgggc
     2641 tgtgttacaa agaacatagt tgcaaatata ttccgtgagt gtaaaacgac tgccggagct
     2701 gcgcggtaag gtgcggcgtc cggcagtcgc tctgcagaca gaaaaaaagg aggggtggat
     2761 gcgcttatga atctctggca gaaaggaaaa cgaaggatgc tgcgcagcgt tcgaatacgc
     2821 agtttgaagg atacgtccct gcggcttgga actccggttt ttctggtcct gatggggttt
     2881 gttgtctgcc tgctctgggg cgttggggaa aaccgcaagc tggcagataa atatattgcc
     2941 gatacggcaa agctctatgt cgatcagatc aaccgtgata tgtcgcagat caacagtgaa
     3001 ctgatctatc tgctggattc tgacagcaac atcaaagaaa tcccggatca gataacatcg
     3061 acagatgctc agtattatga aatggagcag acgctgcggg agcgaaaccg cgtgctgaag
     3121 atccgttacc atgagataca gaccttcttt gtctacggcc agaaagccaa tgttctgatt
     3181 acggacagcg gaactatgtt taccgagagt aagggaatga caacgctgaa tcagatgctg
     3241 atgtcctatc tgcagattat gacatccaag gattcgatca gcacacaatg gaccgtgatt
     3301 acgtttggcg acgacgacta tattgtcggc tggtacgcga aaaacaaaaa agtcatcggc
     3361 tgcgtcatca atctgaatct gattttccgg accatccgcg ccaaaacaga ggagtatgat
     3421 gtcattccgt ttatggtgga tgccagaggc cgtgtcatga cgcaggcaga tacgtcggaa
     3481 aaatataaaa atgagattat cgatttttcc aaagcgggaa agaagaagaa aaatgcgacg
     3541 gtctattcct accagcttgg cacggtcgga aagatcaatt tcatggttct gccgggaggc
     3601 ggtattcttg agaatgtact gattatgcag attgcattcg tcgtcctgat cgcggttctt
     3661 ctgattgtct gcgcattgga aattacagca tattaccacc ggattctgga accgctggaa
     3721 aaattcggac aaaaactgga ggagctggaa aaagagcaga gtctgaatga tgatggaagc
     3781 aacaatctgt tagagctgga gtctgtgagc ggcaaattca aggaactgct gcggaaaata
     3841 cagggattga aaattgcaat ctacgaaaaa gagcttgccg aacagaaagc cgagctggaa
     3901 tacacgcagg aacagatcaa gccacacttc tttctgaact gcctgagcct gatccatggc
     3961 attgcggata agaataacga aggcgagatc gttgagatca caacggtgct gtccgactat
     4021 atgcgctaca ttttccagga ttccaagaag cagcgcgatg tggaggaaga gctggaacac
     4081 attgaggcat atatccgcat ccagaagctg cggtacggag aagaagcctt ttcctttgaa
     4141 gcaacgacgg atggtgatat cggagaatgg aaggttccgt ctcttctgct gcagacactg
     4201 gtggaaaact ccgtagtaca cggtgtatcc ctggaccgga agagtgagat ttccctatat
     4261 ctcacaaacg agacgtacga ggacggtgag tacctctaca tctgtatttc cgacaccgga
     4321 aacggatttt cgaaagaggc actggatgca atcgaaaacg acacgcctat catctaccac
     4381 ggccgtcacc atgtcggcat ctccaatata aaacgccgcc tggcgctgat gtacggcgtc
     4441 gtagaggtgc ggctgccgaa aacagaataa agcgaaaaga aaaaaggcgg gaacccggag
     4501 aaacatagag ggttcccgcc tttttgcgtg aaaaaactgc aggagcttcc ggaacctgat
     4561 ggctccggga agctcctgca aagggggaga tgattcaaaa attgttttct gtatcagata
     4621 cgggaaactc cgtcatcggt ataacgctcg taggaagcgc cttcgtatcc gaccatgcgg
     4681 atgctttcct ctttgatatc cggtacggat tctgcaaaat cggcaaccgg gattgcgcag
     4741 cctttgcgga tgaagagcgg aacctcattc agggcaactt ctacatagtg gatacctttt
     4801 ggaagaacct cctggaacat gtttccattg ccgaggaatt ttacaaacat catttcttcc
     4861 ggcagatata cgtaacggcc gatggcattc tgggtgtaaa ccggtgcgat catgatctca
     4921 ttaccgatca tcaactgatc ttctgtattt cttgccagct tgtcgtccgg ataaacgaag
     4981 gcaagcggtt tgaagtacat atcgtcgttt aaagcagcct tcatgtactc gctgtacaga
     5041 tacggaacga ggcggtagcg gacgccgatg acgttgcgga agtcctcgat atgctcaaac
     5101 tggtaaggtt cctgctcacg tgttccaagt gcggcatggt ttctcatcag aggagtgaaa
     5161 acaccaagtg ccagccagcg gagcagcagg tctcttgtgg tatttgcacc gaatccgccg
     5221 aggtcggctc cggtatagag gaaaccgcac atgttgaggg acggcatcat cttcaggttc
     5281 agcaggatgt gtgaccacca cgagcagttg tctccggtcc agattccacc gtaacggtgc
     5341 atgccgatgt aggaagaacg ggagaacatc aggattcgct tatccgggga aattcgttca
     5401 aaagcttcac cggcagaacg ggtcatgttg tagccaaaca gattgtgtac cttgtcatgg
     5461 cggacctgtt cgccgtttac gttgtgatag aagcgtttat agtcctcatg gttgtttgcc
     5521 agtgcctgaa gctttccgcc taccatgtag tgcggaacct ccgggtcacc ttccacatat
     5581 ttctttgcag tctccttgaa atcagcaagg ccttcctggg aatagaagat ggatggctcg
     5641 ttcatgtcat tccagaaggc atcgattccc ttggaaacga gaacatcgta tttgtcacca
     5701 aaccattttc tggcatctgc atttaagaca tccgggaaat gagtccagcc cggccataca
     5761 gtagcttcga agtaggagcc atcctcacgg cggcagaagt agcctttttc acagccctct
     5821 tcgtaaatgt cataaccctc ttcgactttc acaccggcat cgatgatcgg aacgagatgg
     5881 atcttttctt ttttcatatc ctgtacaaat ccctcgaaat ccgggaaacg ttcttcattt
     5941 acggtgaagt ccttgtaatc ctgcatgtag tcaatatcca tgtaaaccat atcgagcggc
     6001 acatggtttt cgcggtattt tttcactacc gtgcggaaat catcctctgt tttgtagccc
     6061 cagcggctct gaccgtaacc gaaagcaaac ttcggagcaa tgtagctgcg tccgataatg
     6121 ccacggaact gttttacgat atcaagatcg gaatcaccgg tgatcatgta aacgtagaga
     6181 tctgcgtttt cgcaggaaac ggtcatcgta tcggaagttg tgtagccgat atcgaaacgc
     6241 agggtgcccg gataatcgaa gaagaatcct acgtgtgtct tgccggagat gatgatgaag
     6301 ttgtgtgctg catacagaga ggttttgctc tctacgtggt ttggatcatc agcgcagtca
     6361 ctgatgtaaa gaaagccgcg tttgttgata ccgcggttgg cttcgccaag tccgaatacc
     6421 atgtcatcgt cagcaagctt tgtggtgaag gaaaagccgt tctcggtgga gaaggtaccg
     6481 taatccgggt ttccttcgga agaggggatc tcttttacaa cggcttcggt ttcaaatggg
     6541 gttccgtaga caaatttttt taccatgtca tcctccttgt aacataaatg gaaaatatag
     6601 tgttttggtg aggtttagcg caacaccttt ataattttat tataacaggg atcaggaaaa
     6661 tgtctagcaa aaatcatcct ttttttaacc gaatcaagta agtcccctgc ttc
//
LOCUS       DAOMUF010000111         6692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3064_length_6692_cov_3.755462, whole genome shotgun sequence.
ACCESSION   DAOMUF010000111 DAOMUF010000000
VERSION     DAOMUF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_6692_cov_3.755462"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1126)
                     /gene="ychF"
                     /locus_tag="PLN77_07190"
     CDS             complement(29..1126)
                     /gene="ychF"
                     /locus_tag="PLN77_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRM44091.1"
                     /translation="MKLGIVGLPNVGKSTLFNSLTKAGAESANYPFCTIDPNVGIVPV
                     PDKRLQQLGDFYQSKKVTPAVIEFVDIAGLVKGASKGEGLGNQFLANIREVDAIVHVV
                     RCFEDPNVIHVDGSIDPLRDIETIDLELIFADLEVLERRISKTAKSARMDKEAAKELE
                     FQERLKKLLEDGKDAILMELQNEDEEKWIAEYNLLTSKPVIYAANVAEDDLADDGANN
                     SYVQSVREYAKEHNSEVFVICAEIEAEISELEEDEKKMFLEDLGLTESGLEKLIAASY
                     RLLGLMSFLTAGEDETRAWTIKVGTKAPQAAGKIHTDFERGFIKAEVVNYQDLLDCGS
                     YAGAREKGLVRMEGKDYIVRDGDVILFRFNV"
     gene            complement(1251..1904)
                     /locus_tag="PLN77_07195"
     CDS             complement(1251..1904)
                     /locus_tag="PLN77_07195"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRM44092.1"
                     /translation="MRTCIISGGMIEPDFAFPYLQKQQFDIILAADRGLEFCYHYQII
                     PTYILGDFDSLSPEILNYYKENHVAPIREFNPMKDNTDTDIAFQQAIVLGSSEITILG
                     ATGGRLDHFLSIVQNLKTAWEKKIPAYIVDSRNLITIPVETSFEIRKEEQFGKYVSFF
                     PLEKEVASITLEGFAYPLDHHYLPNTSGGLCVSNEIVEETAHVSYEGGILLMVQSRD"
     gene            complement(1939..2622)
                     /gene="rpe"
                     /locus_tag="PLN77_07200"
     CDS             complement(1939..2622)
                     /gene="rpe"
                     /locus_tag="PLN77_07200"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM44093.1"
                     /translation="MSRVYQLAPSILSADFNRLGEQIQRVEKAECRWLHIDIMDGMFV
                     PSISFGMPVVKSIRKESSLYFDVHMMVKDPERYVEEFQSCGADMITVHAEACRDLAET
                     VKKIHETGADAGVAVNPDTPLSAVTDVMDQVEMILIMSVYPGFGGQKYIPESTERIRT
                     LRRMLDEKGLEHVHIQVDGGINRATIDEVLAAGANIIVAGSAVFGSNIEQNVRELQEK
                     ISSYSKKAE"
     gene            complement(2624..3508)
                     /gene="rsgA"
                     /locus_tag="PLN77_07205"
     CDS             complement(2624..3508)
                     /gene="rsgA"
                     /locus_tag="PLN77_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRM44094.1"
                     /translation="MTGKIMKGIAGFYYVDVEESGIYECKAKGIFRKEGQKPLVGDLV
                     EIEILDEAEKTGNMTRILPRKNELIRPAVANIDQALVIFALENPTPNLTLLDRFLVMM
                     EQQNVPTAICFNKRDLAGEDYTDHLRSVYENCGYRVFTVSAAKEQGMQEVEAYLKGKT
                     TVVAGPSGVGKSSITNRMQKEIQMETGEISKKLKKGKHTTRHSQMIPIDHETYLCDTP
                     GFSSLYTTDMEKEELKNFFPEFHPYEGKCRFLGCIHGKEPGCAVKEALEQGNISKERF
                     ENYTMFYEELKEQEKRRY"
     gene            complement(3511..5778)
                     /gene="pknB"
                     /locus_tag="PLN77_07210"
     CDS             complement(3511..5778)
                     /gene="pknB"
                     /locus_tag="PLN77_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HRM44095.1"
                     /translation="MLKEGVILGERYEIISRIGSGGMADVYKAKDHKLNRMVAVKVLK
                     PEFREDKTFIRKFRTEAQAAAGLSHPNIVNVYDVGEDRGVYYIVMELVEGITLKDYID
                     RKGKLSVKEATSIAIQVSLGLEAAHNRNIVHRDVKPQNIIISTDGKVKLSDFGIAKAT
                     SSNTISSNVMGSVHYSSPEQVRGGYSDYKSDIYSLGITMYEMVTGRVPFDGDTTVAIA
                     IKHLQEEIESPSKYTPNLPFALEQIILKCTQKSPDRRYNNMAELIDDLKHSLMEPQGN
                     FVKVTPLSSHAETVMVSAEELSQIRSGAAAKANAQTAEEPARKRYSKIEDEEEDEDYS
                     YEDEEDEDEDEEEDDRDSYDEDDDDSYYDDDDDDDKAGNKLEKIITIGGFAIAAIIIC
                     MLIYFIAQAAGIVGGKGPTAASSSSASSSSSTEVVSSSSKEEEKVTVPDLTNKTEADA
                     VDLANSLNLGVVNGGTEASDVIEAGKICRQDLKPGTKVDKNATITYYVSSGKETVTIP
                     TGIVGTQKDAASAALTQLGLQVSTTEDFSDTVAEGLVISVDPSEGSQVAPESTVTLVI
                     SKGSKDAEEIAIRDVIGLDEETAKSILSDFNIIVKTGTSSKVAEGEVMEVDPAIGTKV
                     KKGSDVTIYVNSPSAQPTTPDENTPTPTPAEPGANTATDVTWSCSQTLSAPDQYQGGI
                     AKIELIQDDGNGNETSITIMDGQAITSWPYSVNTNWSASSSASSTGRIVFYEMDSSGS
                     YQAIAQYQSVPLSEG"
     gene            complement(5772..6515)
                     /locus_tag="PLN77_07215"
     CDS             complement(5772..6515)
                     /locus_tag="PLN77_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRM44096.1"
                     /translation="MKVFSATDIGQKRTMNQDFVFASASPVGNLPNLFVVADGMGGHN
                     AGDFASRYGVSVLVESVKRDTNFNPVRIIRHGIEAANSQIFEQAKRDISMEGMGTTMV
                     VATVVGNYAYVANVGDSRLYLAADGTFDQVTQDHSLIAEMVRLGELTPEQARNHPDKN
                     IITRAVGTQDTVKIDFFDIQLEEGNQLVMCSDGLSNMVTDEEIFELICREDGEDKAKR
                     LIDKANANGGRDNIAVIVVEPFTNEVKEC"
     gene            complement(6546..>6692)
                     /locus_tag="PLN77_07220"
     CDS             complement(6546..>6692)
                     /locus_tag="PLN77_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504211.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRM44097.1"
                     /translation="TKKVVENFKNKLEKYGINVTIRREMGADIDGACGQLRKSFIDKS
                     NEQP"
BASE COUNT         1410 a         1789 c         1496 g         1997 t
ORIGIN      
        1 gaagcggggg acttcttgtc tactggtgtt aaacgttgaa acggaacagg atgacatcgc
       61 cgtcgcggac aatgtaatct tttccttcca tacgcacaag gcctttctct cttgctcctg
      121 cgtaggaacc gcagtcgagc agatcctgat aattgacaac ctcggctttg atgaatccgc
      181 gctcgaaatc ggtgtggatt tttccggctg cctgcggtgc ttttgttccg actttgatgg
      241 tccatgcacg ggtctcatcc tctcctgctg taaggaagct catgaggccg agcagacggt
      301 agcttgctgc gatcagtttt tccagaccgg actcggtaag accaagatcc tcgagaaaca
      361 tttttttctc atcctcttcg agttcggaga tttctgcctc gatctcggcg cagatgacga
      421 atacttcgct gttgtgctct tttgcatatt cgcggacgga ctgtacatag gagttgtttg
      481 caccgtcgtc agcgagatca tcctctgcta cgtttgctgc gtagatgacc ggtttgctgg
      541 tcagcaggtt gtactctgcg atccatttct cctcgtcctc gttctgcagc tccatcagga
      601 tggcatcttt tccgtcctcg agcagttttt tcagacgctc ctggaattcg agctcttttg
      661 ctgcctcttt gtccattctt gcagattttg cagttttgga aatacggcgc tcgagtacct
      721 caaggtcggc gaagatcagc tcaaggtcga tggtctcgat atcacggagc ggatcaatgc
      781 tgccgtctac gtggataacg ttcggatcct cgaaacatct gacaacgtga acgatagcgt
      841 ctacctcacg gatattggca aggaactggt ttccaagtcc ctctcctttg gatgcacctt
      901 ttacaagacc tgcgatgtca acgaactcga tgacagccgg tgttactttc ttggactgat
      961 agaaatctcc gagctgctgc agacgcttgt ccggaaccgg cacgatgccg acattcgggt
     1021 caatggtaca gaacggatag tttgcgctct ctgctcctgc tttggtcagg gaattgaaaa
     1081 gtgtactttt tccgacgttc ggaaggccca cgatacctaa tttcatagta atctatatct
     1141 cctttgtttt tatttttcgc ttctttttct gttcgggttc tttttatgca caaaaagagc
     1201 ccaatgcaac tataaattat agcacacggg ctctttgatt tcaatggatt ttaatctctt
     1261 gactgtacca tcagaagaat gccgccctcg tacgatacat gcgcggtctc ctcgacgatc
     1321 tcattgctca cgcacagacc acccgaagta ttcggcagat agtgatggtc gagcgggtag
     1381 gcgaagccct ccagggtgat ggatgcgact tccttttcca gcgggaagaa cgaaacgtac
     1441 tttccgaact gctcttcttt ccggatttca aacgaagttt ccaccggaat cgtaatcaga
     1501 ttgcggctgt ccacgatgta agccggaatt ttcttctccc atgccgtttt caggttctgc
     1561 acaatactta agaaatggtc gagtcttccc cctgttgctc cgaggatcgt gatttcactg
     1621 cttcccagaa caatcgcctg ctgaaatgcg atgtccgtgt ccgtattgtc cttcatggga
     1681 ttaaattccc ggatcggtgc gacatggttc tccttgtaat aatttaaaat ctccggagat
     1741 aaactgtcaa aatctcccag aatatacgtc ggtataatct ggtaatggta gcagaactcc
     1801 agtccccggt ctgctgccag gataatatca aactgctgtt tctgaagata tgggaaggca
     1861 aaatccggtt cgatcatccc tcccgaaata atacatgttc tcacgtttcc ctgcctcttt
     1921 tttctgtctt tttgtatctt attctgcctt tttgctgtat gaagaaattt tttcctgcag
     1981 ctcgcgcacg ttctgctcga tgttgctgcc aaatacagca gagcctgcca cgatgatgtt
     2041 ggcacctgcc gcaaggactt catcgatggt cgcgcggttg atgccgccat cgacctgaat
     2101 gtgcacatgc tccagtccct tttcatccag cattctgcgg agcgtacgga tcctctccgt
     2161 cgactccggg atatacttct ggccgccgaa gccaggataa acgctcataa tcagaatcat
     2221 ttctacctga tccatcacgt ccgtaaccgc agaaagcggc gtatccggat ttacggcaac
     2281 tccggcatct gcgcctgttt catgaatctt tttcacggtt tctgccagat cgcggcatgc
     2341 ctccgcgtga accgtgatca tatccgcccc gcagctttga aattcttcta cgtaccgctc
     2401 cgggtccttt accatcatat gaacatcaaa atacagactg ctttcttttc ggatcgactt
     2461 taccaccggc attccaaacg aaatggatgg tacaaacatt ccatccataa tatcgatatg
     2521 aagccaccgg cactctgctt tctctaccct ttggatctgt tctccgagac ggttgaagtc
     2581 tgcggataaa atagaaggtg ccaactgata tactcggctc atgctagtat ctcctctttt
     2641 cctgttcttt cagttcttca tagaacatgg tatagttttc aaatctttct ttgctgatat
     2701 ttccctgttc cagtgcctct ttgacggcac agcccggctc ttttccgtgg atgcagccga
     2761 ggaaacggca ttttccttcg tacggatgaa attccgggaa aaagtttttc agctcctctt
     2821 tttccatatc ggttgtataa agcgagctga accccggcgt atcgcacaga tacgtctcat
     2881 ggtcgatcgg gatcatctgg gaatggcgcg tcgtgtgttt tccttttttc agctttttgc
     2941 tgatctctcc ggtctccatc tggatttctt tctgcatccg gtttgtgatc gaagattttc
     3001 cgacgccgga cggaccggct accaccgttg ttttcccttt caggtatgct tccacttcct
     3061 gcatgccctg ctctttcgct gcactcaccg taaagacacg gtaaccgcag ttctcataga
     3121 cgcttcgcag atggtctgtg taatcttccc ccgcaagatc tcttttattg aaacagattg
     3181 ccgtcggaac gttctgctgt tccatcatca caagaaaacg gtccagaagc gtcaggttcg
     3241 gggtcggatt ttccagcgca aaaatcacaa gcgcctgatc gatattggcc actgccggac
     3301 ggatcagttc attttttcgc ggcagaatcc gggtcatatt tcctgttttt tccgcttcat
     3361 ccagaatctc gatttccaca agatctccga cgagcggttt ctggccttcc tttcggaaaa
     3421 ttccttttgc tttgcactca taaatgccgg attcttctac gtctacgtag tagaatccgg
     3481 caattccttt cataatcttt cctgtcatac tcacccttca ctcaacggaa ccgattggta
     3541 ctgtgcaatt gcctgataac ttccgctcga atccatctca tagaagacga ttcttccggt
     3601 agaagaagca gacgatgaag cactccagtt ggtgttgaca gaatacggcc agctcgtaat
     3661 ggcctgtccg tccatgatgg tgatggaggt ctcgtttccg ttcccatcat cctggatcag
     3721 ctcaattttt gcaataccac cctgatactg atccggcgca cttaaggtct ggctgcagga
     3781 ccaggtaaca tccgtagcgg tatttgcgcc cggctctgcc ggtgtcgggg tcggtgtatt
     3841 ctcatccggc gtcgttggct gtgcactcgg gctattgaca taaatcgtaa cgtccgaacc
     3901 tttcttcact ttggttccga ttgccgggtc aacctccatg acttcaccct cggccacctt
     3961 gctgctggtt cctgttttta caataatatt gaaatcgctc aggattgatt ttgctgtctc
     4021 ctcatccagg ccgattacat cacggattgc aatttcttcc gcgtccttgg atcccttgct
     4081 gatcaccagc gtaacggtag attccggcgc aacctgactt ccctctgacg ggtcaacgga
     4141 aataaccaag ccttctgcaa cggtatcgct gaaatcctct gttgtggaaa cctgcagtcc
     4201 cagctgagtc agtgcagcac ttgcagcgtc tttctgtgtt ccaacaatgc cggtcggaat
     4261 ggttacggtt tcttttccgg agcttacata atacgtaatc gttgcatttt tatcgacttt
     4321 cgttcccggt ttcagatcct ggcggcagat ctttcctgcc tcaatgacat ccgatgcctc
     4381 ggttccgccg ttgactacac caaggttcag gctgtttgcc agatcgacgg catctgcctc
     4441 ggttttgttg gtcagatccg gaacggttac tttttcctct tctttgctgc tggaagaaac
     4501 aacttccgtg ctgctcgaag atgatgcgga actggaagaa gctgcggtcg gtccttttcc
     4561 gccgacaatg ccggctgcct gtgcaataaa gtaaatcagc atgcagatga tgatggctgc
     4621 aatcgcaaaa ccgccgattg tgatgatctt ttcgagtttg tttccggctt tgtcatcgtc
     4681 gtcgtcatcg tcgtagtagg aatcgtcgtc atcctcatcg taggaatcgc ggtcatcctc
     4741 ttcttcgtcc tcatcctcgt cttcttcgtc ctcgtagctg taatcttcgt cctcttcctc
     4801 atcctcgatc ttgctgtagc gcttgcgggc cggctcctct gccgtctgcg catttgcctt
     4861 tgctgctgcg ccggaacgga tctggcttaa ctcctctgcg gataccataa cagtctcggc
     4921 gtgagaactt aacggagtta cttttacgaa attgccctgc ggctccatca gcgaatgctt
     4981 caagtcgtcg atcagctccg ccatgttatt gtaacggcgg tccgggctct tctgggtaca
     5041 ttttaagata atctgctcca gtgcgaacgg aaggttcggg gtatatttgg atggagattc
     5101 aatctcctcc tgcagatgct tgattgcgat ggcaacggtg gtgtcgccgt caaacggcac
     5161 tcttccggtt accatctcgt acatggtgat accaagggaa tagatatcac tcttatagtc
     5221 cgaatatccg ccgcgcacct gctccggaga gctgtaatgt acgcttccca taacgttcga
     5281 actgatcgta ttggaagaag ttgcctttgc aataccaaag tcggaaagtt ttacttttcc
     5341 gtctgtggaa atgatgatgt tctgcggttt gacatcgcgg tgtacaatat tgcggttgtg
     5401 tgctgcttca agaccaaggg aaacctgaat ggcgatgctg gttgcctctt ttacgctcag
     5461 ttttcctttg cggtcaatgt aatccttcag agtaattccc tcgacaagct ccatgacgat
     5521 atagtaaacg ccgcggtcct ccccgacatc gtacacgttt acaatattcg gatgggacag
     5581 tcctgctgcg gcctgtgcct ccgtgcggaa cttgcggata aaggttttat cctcccggaa
     5641 ttccggtttt aaaaccttga cggccaccat gcggttcagc ttgtggtctt ttgctttgta
     5701 gacatcagcc ataccgccgg aaccgatccg gcttatgatc tcatatcgtt ctcctaaaat
     5761 aacgccctct tttaacattc tttcacctcg tttgtaaatg gttcaacgac aatgactgcg
     5821 atattgtctc tgccgccatt tgcatttgct ttatcaatta aacgtttcgc cttgtcctca
     5881 ccgtcttctc ggcagatgag ttcgaaaatc tcctcatccg ttaccatgtt ggaaaggccg
     5941 tccgagcaca tcaccaattg attgccctct tcaagctgaa tgtcaaagaa atcaatcttt
     6001 acggtatcct gtgtgcctac tgctctggta ataatgtttt tgtccgggtg attccgcgcc
     6061 tgctcgggag tgagttctcc aaggcgcacc atctctgcga tcagggaatg gtcctgcgtc
     6121 acctggtcaa acgtgccatc tgctgccaga tacagcctgc tgtctcctac gtttgcaacg
     6181 taggcatagt tgccgacaac cgtagctact accatcgtcg tacccatgcc ttccatggat
     6241 atatctcgtt ttgcctgctc aaaaatctgg ctgtttgctg cttcaattcc atgccgaatg
     6301 atccgcaccg ggttgaaatt cgtatccctt tttacagatt ccaccagtac agaaacaccg
     6361 taacgcgaag caaaatcgcc cgcgttatgg cctcccatgc cgtctgcaac gacaaagagg
     6421 ttcggaaggt ttccgaccgg agaggcggag gcaaacacaa aatcctggtt catcgtccgt
     6481 ttctgtccga tatcggttgc agaaaatacc ttcatgcttt ttctcctgta ttgacagccg
     6541 cttttttacg gctgttcgtt tgatttatca ataaaacttt ttcttaactg tccacaggct
     6601 ccgtcaatat cagcgcccat ttcccttcta atagtaacat taattccgta tttttcaagt
     6661 ttatttttga aattctctac gacttttttc gt
//
LOCUS       DAOMUF010000112         6593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3134_length_6593_cov_3.132762, whole genome shotgun sequence.
ACCESSION   DAOMUF010000112 DAOMUF010000000
VERSION     DAOMUF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3134_length_6593_cov_3.132762"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLN77_07225"
     CDS             <1..405
                     /locus_tag="PLN77_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRM44098.1"
                     /translation="REYLYVTADTYSIVRLLPKAAELLDEDGTMLMKMAKEEKRIPKA
                     AKEKTKTKTSAAAKDLSNEEAAVFEKLRALRMELAKEHKVPPYIIFSDKTLIQMAMEK
                     PSNKEEMLAVSGVGESKFEKYGEPFLACLAER"
     gene            457..1338
                     /locus_tag="PLN77_07230"
     CDS             457..1338
                     /locus_tag="PLN77_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44099.1"
                     /translation="MKKENAVIFGVTGVVFAAALIGGGIYMKTERDRNLNADTASTAA
                     DSNRAEEVQKAVFLAEDSGLWYLGDLEHGNIYVTHTPSDTLYDENGNAIDPSEIKKGD
                     FLQVEGDGIMLNSYPGQYPGISRIMRISGGTEADAEKFDEELSRILPEKDPSEIPFLN
                     LCYTQPNAQVTAMATQGGYTWSYVDEDGNGQNVVADSAFILEWTELNDLNTANDEGKT
                     DLELVFSEEPDSVTAERWPAEDRGQNFGNGYPEGESVSVEHAESWSIPGAEAGYIYEV
                     DAVFENGTVSYGFEVKK"
     gene            1476..1814
                     /locus_tag="PLN77_07235"
     CDS             1476..1814
                     /locus_tag="PLN77_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HRM44100.1"
                     /translation="MKLIVAVLRKEDEADTIAELNKKGFFVTELATTGGFLKEKNTTL
                     LIGTDDHKLDAVLAILKQEAGLRKTVTYAATPMESGNFCPGGNYSVPIDTQTGGCTVF
                     VLNMEQLEKF"
     gene            complement(1819..2586)
                     /locus_tag="PLN77_07240"
     CDS             complement(1819..2586)
                     /locus_tag="PLN77_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HRM44101.1"
                     /translation="MTVYTCSPDLASILTCIYEAWNSRLGYRNVRLMTEPVGNLELFC
                     DYCHVEPDTEKAASVTRSIQKKIGAAAWRLVYLCAMSERSDAPDIIYRFLLYGFSYGK
                     DTLHMLQEPAVFHAFEVSRQVTNEAHSFREFIRFANISSGFPILVSHISPKANVLTLV
                     APHFSDRLPSENWMILDDNRQLAVVHPADRPFYLTRLSPDEIERLSLTESTSDPYEEL
                     WKGFFTAIAIKERSNPDCQRTHLPLHYRDHMPEFKNP"
     gene            complement(2686..4125)
                     /locus_tag="PLN77_07245"
     CDS             complement(2686..4125)
                     /locus_tag="PLN77_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HRM44102.1"
                     /translation="MQLSSQTLSLSEKLNLLADAAKYDVACTSSGVNRAGKQEHLGNS
                     VSCGICHSFSSDGRCISLLKILQSNDCIYDCKYCVNRAGNDRQRATFTPEEVCTLVTE
                     FYRRNYIEGLFLSSAVCKNPAHTMELMYRTLHLLRNRYRFNGYIHVKAIPGAPAELIE
                     KTGYLADRMSVNLELPTGEGLQKLAPQKTQKAILKPMRQIQSGIVDYRLTAGKSAFLE
                     RSSGNRYLSHSIFDTRKQISASAADPGWISSSSARSLPEKERSAPFVPAGQSTQMIIG
                     ATKENDYQLLSTSQLLYQQYDLKRVFYSAYIPVNEDSALPSRETKPPLLREHRLYQAD
                     WLLRFYGFQADELLDEAHPNFHAQLDPKCDWALRHMELFPVEINTAEYADLLRVPGIG
                     PKSAMRIVKSRRSSHLSFEHLKKIGVVLKRAKYFITCEGKMMYHMRLEQQFLTRQLTG
                     EEAASNWEVSHPEQYQQLSLFDAAVPGHI"
     gene            4261..4410
                     /locus_tag="PLN77_07250"
     CDS             4261..4410
                     /locus_tag="PLN77_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44103.1"
                     /translation="MKIVQENTGRKETEFDPEKREQETAGKISVRKKLYKNSRTDRKN
                     KSVRL"
     gene            complement(4448..4927)
                     /locus_tag="PLN77_07255"
     CDS             complement(4448..4927)
                     /locus_tag="PLN77_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HRM44104.1"
                     /translation="MITKYLIVFLISMVPLIELRGAIPYAVAFHIPTLPAYLIAVIGN
                     MLPVPIIFLFARKVLVWGCDKKYIGKFFTWCLEKGEKGGEKLKAKAGRGLPLALLLFV
                     GIPLPGTGAWTGTLAASILDMDFKTSVLAVMGGVLLASVIMLLASSGVFGAIGALLT"
     gene            5206..5823
                     /gene="hisH"
                     /locus_tag="PLN77_07260"
     CDS             5206..5823
                     /gene="hisH"
                     /locus_tag="PLN77_07260"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRM44105.1"
                     /translation="MIALIDYDAGNIKSVEKAFQSLGKEVVLTRDPEVLKRADHVVLP
                     GVGSFGDAMENLNRYHLVPVIHEIVEKGTPFLGICLGLQILFERSEESPGVEGLGLLK
                     GEILRIPDKEGLKIPHMGWNSIHLQNEGRLFKGISQESYVYFVHSYYLKAEEEEIIKA
                     TTEYSTHIHASVEKGNLFACQFHPEKSSDLGLAILKNFAEIGEED"
     gene            5827..6588
                     /gene="hisF"
                     /locus_tag="PLN77_07265"
     CDS             5827..6588
                     /gene="hisF"
                     /locus_tag="PLN77_07265"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRM44106.1"
                     /translation="MFTKRIIPCLDVKNGRVVKGVNFVDLKDAGDPVEIAKAYDKAGA
                     DEVVFLDITASSDQRGTVIDMVRKVAANVFIPFTVGGGIRTVDDFKMLLREGADKISI
                     NSSAINNPQLIHDAAQKFGSQCVVVAIDAKKRADGSGWNIYKNGGRIDVGIDAVEWAK
                     KAESLGAGEILLTSMDCDGTKAGYDIELTRTIAESVGIPVIASGGAGTLEHFYDALTE
                     GKADAALAASLFHYKELEIREVKEYLAGRNVPVRL"
BASE COUNT         1901 a         1451 c         1816 g         1425 t
ORIGIN      
        1 cgggaatatc tctatgtaac ggccgacaca tacagcattg tgcgcctgtt gccaaaagcg
       61 gcggagctgc tggatgagga cggcacgatg ctgatgaaga tggcaaaaga agaaaaacgg
      121 attccgaaag ccgcaaaaga gaagacaaag acaaaaacct cggcagcggc aaaggatctt
      181 tccaacgagg aggccgcggt attcgaaaaa ctgcgggcac tccggatgga gcttgcaaag
      241 gagcataaag tgccgccgta tattatcttc tcggataaaa cactgatcca gatggcgatg
      301 gagaaaccgt ccaacaaaga ggaaatgctg gcggtctctg gtgtcggcga gagcaaattt
      361 gaaaaatacg gggaaccgtt tcttgcatgt cttgcagaaa gataaaacaa gtcgtgtaaa
      421 aaaacgggaa aacaacagga aaggaagaat acgcttatga aaaaagaaaa cgcagttatt
      481 tttggagtga ccggtgttgt atttgccgcc gcgctgattg gaggcggaat ctatatgaaa
      541 acggaacggg accgaaatct gaatgcagat acagcaagta ctgcagcgga cagcaatagg
      601 gcagaagaag tacagaaagc cgtatttctt gcagaagatt ccgggttatg gtacctggga
      661 gatctggaac acggaaacat ctatgtgaca catacaccgt ccgacacact ttacgatgaa
      721 aatggaaatg ccattgatcc gtccgagatc aaaaaaggag attttcttca agtggagggc
      781 gacggtatta tgctgaactc ctatccggga caatatccgg gcatcagccg tattatgaga
      841 atcagcggcg gtacggaggc ggatgcagaa aaattcgatg aggaactttc ccggattctg
      901 ccggaaaagg atccatcgga aattccgttt ctgaatctgt gctacacaca gccaaatgcc
      961 caggtgacag ctatggcaac acagggaggt tatacctggt cttatgttga tgaggacggc
     1021 aatggtcaga acgtcgttgc ggacagtgcg ttcattctgg aatggacgga actgaatgat
     1081 ctgaatacag caaatgatga aggaaaaacc gatctggagc ttgtcttctc ggaggagccg
     1141 gattctgtca cagcagaaag atggccggcc gaagaccggg ggcagaactt tggcaatggt
     1201 tacccggagg gagaaagcgt ttctgtggag catgcggaga gctggagcat tccgggagcc
     1261 gaggccggct acatctacga agtggacgcg gtattcgaaa atggaaccgt gtcatacgga
     1321 tttgaagtga aaaagtaaaa aaagactttc tttttctgtg acattatgct atttttactg
     1381 tgaaaacaga aaaatgcaat gtgccatacg gcataagcag ctgacttttc aaaaatcaga
     1441 atccgtttga ccggaggatg cgaggaggaa tttgtatgaa gttaattgtt gctgtattac
     1501 ggaaagagga cgaggcagac accattgccg agttgaacaa aaaaggcttt tttgtgacgg
     1561 agcttgccac gaccggcggt ttcctgaagg agaagaacac aacactgctg atcggaaccg
     1621 atgatcataa gctcgacgct gtgcttgcga tcttaaaaca ggaagcaggg cttcggaaaa
     1681 cagttaccta tgcggccacc ccgatggaaa gcggaaactt ctgcccgggc ggaaattact
     1741 ctgttccgat tgatacccag acaggaggct gtactgtgtt tgtgctgaac atggaacagc
     1801 tggaaaaatt ctaagactct aaggattttt aaattccggc atatgatccc ggtagtggag
     1861 cggcagatgc gtacgctgac agtcgggatt ggaacgctct ttgatggcaa ttgcggtgaa
     1921 aaatcctttc cagagttctt cgtaaggatc cgaagtggat tccgtgaggg aaaggcgttc
     1981 tatttcatcc ggagaaagac gggtcagata aaagggccgg tcagcgggat gcacgacggc
     2041 aagctgccgg ttatcatcga gaatcatcca gttttctgag gggagccggt cagaaaaatg
     2101 gggagcgacc agggtcagca cattggcttt cggggaaata tgcgagacca ggatcggaaa
     2161 accggaggaa atatttgcaa aacggataaa ttcccggaac gaatgcgctt catttgtgac
     2221 ctgacgggaa acttcaaaag cgtggaatac cgccggttcc tgcagcatat gcagggtatc
     2281 ctttccatag gaaaagccgt agagcagaaa acgataaatg atgtcgggag cgtcgcttct
     2341 ctcggacatt gcacagagat agacgagccg ccaggctgct gcgccgatct tcttttgaat
     2401 ggatcgcgtg acgctggcgg ctttttctgt gtccggctca acatggcagt aatcgcagaa
     2461 cagctccaga ttgccgactg gttccgtcat cagccgtaca ttgcggtagc cgagacggga
     2521 attccaggct tcatagatgc aggtcaggat cgacgcaaga tccgggctgc aggtgtaaac
     2581 agtcataata aaatcctttc tgaaatcaat tgatacagac aacagccttc tgcaaaaaag
     2641 cgcagataca gcaaaaaggc ttttccgtga gaagaacgcc cgggattata tatgcccagg
     2701 tacggcagca tcaaacagag aaagctgctg atactgttcc ggatgggaaa cctcccagtt
     2761 ggaggcggct tcctctcccg tgagctgccg ggtcagaaac tgctgttcca gccgcatgtg
     2821 atacatcatt tttccctcgc aggtgataaa atatttggcg cgtttcagca ccacaccgat
     2881 ctttttcagg tgctcaaatg aaagatggga gcttctgcgg ctctttacaa tccgcatggc
     2941 cgatttcggc ccgatgccgg gaacgcgcag aaggtcggca tactcagccg tgttgatctc
     3001 caccggaaac agctccatat ggcgcagcgc ccagtcgcat ttcgggtcga gctgtgcatg
     3061 aaagttcggg tgggcttcat cgagcagttc atccgcctga aaaccgtaga agcggagcag
     3121 ccagtctgcc tgataaaggc ggtgttcccg aagaagcggc ggcttggttt cacgtgaggg
     3181 cagggcggaa tcctcgttga ccggaatgta ggcggagtaa aagacgcgct tcaggtcata
     3241 ctgctggtag agaagctgtg aggtggagag aagctggtaa tcgttttcct ttgtcgcacc
     3301 gatgatcatc tgcgtgctct ggccggcagg aacgaaggga gcagagcgtt ctttttccgg
     3361 cagtgaccgc gcggaggagg aagagatcca gcccggatcg gcagcggagg cggaaatctg
     3421 ctttcgtgta tcgaaaatgg agtgggagag atagcggttt cccgaggatc gctctaaaaa
     3481 agcgcttttc cctgcggtca gccggtaatc tacaataccg gactggatct ggcgcatggg
     3541 ttttaaaatc gctttctgtg tcttctgcgg ggccagtttc tgcagccctt ctccggtcgg
     3601 gagttccaga ttgacgctca tgcggtcggc gagatagccg gtcttttcga tcagctcggc
     3661 gggggcgccc gggatagctt tgacgtggat gtagccgtta aagcggtaac gattacgtaa
     3721 aagatgcaga gtgcggtaca tgagctccat cgtatgggca ggatttttgc agacggcgga
     3781 acttaaaaag agtccctcaa tatagtttcg gcgataaaat tccgtgacga gtgtacagac
     3841 ctcctccggt gtaaacgtgg cgcgctgccg gtcattcccg gcgcggttga cgcagtattt
     3901 acagtcgtag atacagtcgt tgctctgcag aattttcaac agagagatac agcgtccgtc
     3961 ggaagaaaag ctgtggcaga taccgcagga gaccgaattg ccgagatgtt cctgttttcc
     4021 ggcacggttc acaccgctgg aagtgcacgc aacgtcgtat ttggccgcgt cagcgagaag
     4081 attcagcttc tcggagagag aaagtgtttg tgatgataac tgcatggcag aaaaattcct
     4141 ttctatgtcc aaatgaatac gaataaaagt aaaacaataa gataacaggt attttatgaa
     4201 aagaacaact gttcgataaa agaagtgtaa cacaaatcga acgaatgttc aagagaaaaa
     4261 atgaaaatag tacaagaaaa tactggaaga aaagaaacag aattcgatcc ggaaaaacgt
     4321 gaacaggaaa cagcagggaa aatttctgtt agaaaaaagt tatacaaaaa cagccgcaca
     4381 gatcggaaaa acaaatctgt gcggctgtaa gccaaatttt atggaattga aacggaagca
     4441 gagccgttta cgtcagaagc gccccaattg ccccgaatac gccggaactt gcaagcagca
     4501 tgatgacgct tgcgaggaga acaccgccca tgacggcgag aacgctggtc ttaaagtcca
     4561 tatcgaggat acttgcggcc agtgtgccgg tccatgcgcc ggttcccggg agcgggatgc
     4621 cgacgaagag cagcagggcg agcggaagtc cgcggccggc ttttgctttc aatttttccc
     4681 cgcctttttc gcctttttcc agacaccagg tgaagaactt tccgatatac tttttatcac
     4741 atccccagac aagaaccttt cgagcgaaca ggaaaataat cggaaccgga agcatatttc
     4801 cgatgacggc gatcagatag gcaggaagcg tcggaatatg gaaagctacc gcgtacggaa
     4861 tcgcaccacg cagctcgatg agcggaacca tggaaatcaa aaatacaatc agatatttgg
     4921 taatcatata cacctctcgt tttttacaaa atgttatcgt ttcttagtgc aacgctctta
     4981 ttgtagcgtg atgcccgcca tttgtccagt ataaaaagaa agaaaagagc gggagtaaaa
     5041 gaaatcttaa gaatttctgt atttttgtat aattttataa ccttcaaaat ttgaatttta
     5101 gaaaactgta tgttagaata aagtgtactg tttgcaaaag aacagcagag atgcccccaa
     5161 tgacaggggg cgtggactga aataacaaac gaggaggacc cacctatgat tgcactgatc
     5221 gactatgatg ccggaaatat aaaaagcgtg gaaaaagcct ttcaaagcct tggaaaagag
     5281 gtcgtactga ccagggatcc agaagtatta aagcgcgcag atcacgtcgt tctgccgggt
     5341 gtcggcagtt ttggagatgc gatggaaaat ctgaaccgct atcatctggt accggtcatt
     5401 catgaaatcg tagaaaaagg gactccgttt cttggaatct gtctcggact gcagatcctg
     5461 tttgaacgca gcgaggagag cccgggcgtg gaaggccttg gcctgttaaa aggagaaatc
     5521 cttcggattc cggacaaaga gggcttaaag attccgcaca tgggctggaa ttccatccat
     5581 ctgcagaacg agggacgctt attcaaaggc atctcgcagg agtcttacgt ctactttgtc
     5641 cactcctact atctgaaagc agaagaggag gaaattataa aagcaacgac agagtacagt
     5701 acacacatcc atgcatccgt ggaaaaagga aatctgtttg cctgccagtt ccacccggaa
     5761 aagagttcag atcttggact tgccattctg aaaaattttg cggaaattgg agaggaggac
     5821 taagcgatgt ttacaaaacg aattatccca tgcctggatg taaaaaacgg aagagtcgta
     5881 aaaggcgtta atttcgtcga tttaaaagat gccggagacc cggtggagat tgcaaaagcg
     5941 tacgacaagg caggagccga tgaagttgtg ttcctcgaca tcaccgcctc ctccgaccag
     6001 agaggaacgg tcatcgatat ggtgcgcaaa gtagcagcaa acgtcttcat cccattcacc
     6061 gtcggcggcg gaatccgtac cgtagacgat ttcaagatgc tgctccgcga gggagccgac
     6121 aaaatttcga tcaactcctc cgcaatcaac aatccgcagc tgatccacga tgcggcgcag
     6181 aaatttggca gccagtgcgt ggtcgttgcg atcgatgcga agaagagagc tgacggaagc
     6241 ggctggaata tctacaaaaa cggcggccgg atcgatgtcg gtatcgacgc cgtcgagtgg
     6301 gcgaaaaaag ccgagagcct cggagccgga gaaattctgc tcaccagcat ggactgcgac
     6361 ggaacaaagg cgggatacga tatcgagctg acaagaacga tcgcagagag cgtcggcatc
     6421 ccggtcatcg catccggcgg cgcaggaacg ctggagcatt tttatgatgc cttaaccgaa
     6481 ggaaaagccg atgcagccct cgccgcatcc ctgttccact acaaagaact ggaaattcgt
     6541 gaagtcaaag aatatctggc aggccgtaat gtgccggtcc gcctgtaaaa gga
//
LOCUS       DAOMUF010000113         6518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3188_length_6518_cov_3.152870, whole genome shotgun sequence.
ACCESSION   DAOMUF010000113 DAOMUF010000000
VERSION     DAOMUF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_6518_cov_3.152870"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLN77_07270"
     CDS             <1..742
                     /locus_tag="PLN77_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44107.1"
                     /translation="ILNQGDRIMIDKMVGKTEVALYGLAYSIGMLVQLFISGMNSALT
                     PWMYSKIKKGDIRSMRKVLQLLSVVVVGIAVALMLISPELVLIFGSPKYREAVYVIPP
                     VAASVFFIFIYNILSMPQFYYEKTQFLMAASMAAAGANVVLNFIFIRLFGYIAAGYTT
                     LACYMLYSVGHYIVSKRILVNEGIQENLMSPGFLVACSVFLIGASIICNLIFPYRILR
                     YAILLASAGLVFWKKELLLKTMADIRKK"
     assembly_gap    985..994
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <995..2055
                     /locus_tag="PLN77_07275"
     CDS             <995..2055
                     /locus_tag="PLN77_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM44108.1"
                     /translation="SDAIDRSKKTIICCSRGRFSVAAAEELQEKGWDAVSLEGGYIAW
                     LMDVMSAPEEQDKAADVEKSLRKKFKKSIWSKFTKAINTYELVKPGDKIAVCISGGKD
                     SMLMAKCFQELKLHNKFDFEVKFVVMDPGYSPENRKVIEDNAKSLHIPITIFETDIFD
                     SVYHVENSPCYLCARMRRGHLYHFAQQLGCNKIALGHHYDDVIETILMGMLYGAQVQT
                     MMPKLHSIHFEGMELIRPLYLVREDDIKAWRDYNGLHFIQCACKFTDTCTTCNNEENR
                     SKRVEIKELIKNLKKVNPFVESNIFRSVENVNIDTVVGYKQHGIRHNFLEGYDDNPGY
                     VPEAEKAAAETEQEKEGK"
     gene            2055..2309
                     /locus_tag="PLN77_07280"
     CDS             2055..2309
                     /locus_tag="PLN77_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM44109.1"
                     /translation="MHRKIEETPGREKMLIRLKELRLERGYSMTQAARKIFSHPDTYN
                     RYEKGIREIPLDSLMLLAKTYDCSLDYLAGVSDRPRKYGR"
     gene            2315..2887
                     /locus_tag="PLN77_07285"
     CDS             2315..2887
                     /locus_tag="PLN77_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM44110.1"
                     /translation="MAIPLYLTTIEQDIFDTFRYLPHAVAVGVGFVTVAAAWKNQQMI
                     KKQKRLEHAHAVPGTQLLLWFLVAVYFAMMLSITLFSREPGSRTGVDVKLFETWGNQR
                     LPDRYFVENILLFLPFGVLLPAAVPSLRKGWCCVYAAFSTSMMLETVQLLTERGYCQL
                     DDVVTNTLGAAIGYLVFALVRKCWRGKIEE"
     gene            complement(3121..4008)
                     /locus_tag="PLN77_07290"
     CDS             complement(3121..4008)
                     /locus_tag="PLN77_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM44111.1"
                     /translation="MSSYVISCCSTADLTEEHFSKREISYICFHYKLNDTEYADDLGK
                     SMPFDQFYAAMAAGASTKTSQVNADEFEAYFETFLQDGKDILHVCLSSGISGVLNSAM
                     IAKADLEERYPERKILIVDSLGASSGYGLLMDRLADLRDEGKPLEEVYDFAMKNRLKV
                     HHWFFSTDLTFYVRGGRISKASGAIGGLLGICPLLNMDNEGRLIPRFKIRTKKKVIKK
                     IVDQMEENAEGGLSYSGKCYISQSACYEDARAVADLIEERFPNLNGKVEINNIGTTIG
                     SHTGPGTVALFFWGKERVD"
     gene            4215..4991
                     /gene="udp"
                     /locus_tag="PLN77_07295"
     CDS             4215..4991
                     /gene="udp"
                     /locus_tag="PLN77_07295"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HRM44112.1"
                     /translation="MQNYSGEEGLQYHLQIRKGDVGRYVILPGDPKRCKKIAAYFENP
                     VLVADSREFVTYTGTLDGVKVSVTSTGIGGPSASIAMEELIQCGADTFIRVGTCGGMA
                     LDVEGGDIVVATGAIRAEGTSKEYAPIEFPAVADLTVTNALVQAAKNLGKKWHAGVVQ
                     CKDSFYGQHDPEQMPVGDELLKKWDAWMKLGCKASEMESAALFIVASARGVRAGSDFL
                     VMGNQERVKRGMENHITHDTEGAIQVAIEALRILIREDQK"
     gene            5033..5464
                     /locus_tag="PLN77_07300"
     CDS             5033..5464
                     /locus_tag="PLN77_07300"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRM44113.1"
                     /translation="MKQEISTEIKQELVKQALAARKFAYVPYSKFRVGAAVLAKNGAI
                     YTGCNIENAAYTPTNCAERTAVFKAVSEGITEFDAIAVCGGPGEAEPEDFCTPCGVCR
                     QVLREFVDPNKFLVLLCKGDGSWKEMTLEELLPMGFGKENL"
     gene            complement(5551..>6518)
                     /locus_tag="PLN77_07305"
     CDS             complement(5551..>6518)
                     /locus_tag="PLN77_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HRM44114.1"
                     /translation="TTRPLVVVTAPGPGSGKMATCLSQLYHEHKRGVNAGYAKYETFP
                     IWNLPLKHPVNLAYEAATADLNDVNMIDPFHLEAYGVTTVNYNRDVEIFPVLRAIFQQ
                     IFGESPYKSPTDMGVNMAGLCIFDDEACREASRQEIIRRYYTAVDGIADGSRSETEAY
                     KIELLMKQENIVAADRTTVSPALVRAEATGAPAAAMELPDGRVITGKTGDLLGSSAAL
                     LLNALKELSGIDHDVHVLSPESIAPIQTLKTKYLGSKNPRLHTDEVLIALSATAAQSE
                     DAQKALSCLPQLKGCQVHSSVMLSDVDRKMFQRLGCDLTCEPKFQ"
BASE COUNT         1627 a         1459 c         1911 g         1511 t
ORIGIN      
        1 tatcctgaat cagggcgacc gcatcatgat cgacaagatg gtcggaaaga cggaagttgc
       61 gctgtacggg ctggcgtaca gcattggtat gctggtgcag cttttcatca gtggtatgaa
      121 cagtgcattg acaccgtgga tgtacagcaa gatcaaaaag ggcgacatcc gctcgatgcg
      181 gaaggtgctg cagcttctta gtgttgtagt ggtcggtata gccgtggcgc tgatgctgat
      241 cagcccggag ctggtgctga ttttcggatc accgaagtac cgcgaggcgg tgtacgtcat
      301 tccgccggtg gcagcgagtg tgttctttat cttcatttat aatattctct cgatgccgca
      361 gttttattat gaaaaaacac agtttctgat ggctgcatcc atggcggcgg ctggagcgaa
      421 tgtggtgctg aactttattt ttatccgcct gttcggttac atcgcggccg gttacacgac
      481 gctggcatgc tatatgctgt attcggtcgg ccattatatt gtgagcaaac ggattctggt
      541 gaacgaagga atccaggaaa atctgatgag tccaggattt cttgtggcgt gctccgtttt
      601 tctgattgga gcaagcatca tctgcaatct tattttcccg tacagaatcc tgcgttacgc
      661 gattctgctc gcaagtgcag ggctggtctt ctggaagaaa gaacttctgc tgaagacgat
      721 ggcggatatc cggaaaaaat aaaaaagtgg gggaggattt gaaacgaatg ttcagagact
      781 ccccgttttt gcgcgttttt tcttgacagt cacagaaaaa acgatacaat gaaagaatga
      841 agaaaagtaa ggcattttaa ttagaaaagg aaaaattaca tgagtaccag tatttcaata
      901 aaagaactaa aagagctcga tccgtcaagc tatcagatcg tcgatatccg cgatgcggta
      961 gagatctccc acggcgcgat tccgnnnnnn nnnncaagtg atgcgattga ccggagcaaa
     1021 aagacgatta tctgctgcag ccgcggccgg ttcagtgtcg cagcggcgga agaattacag
     1081 gaaaagggct gggacgctgt gagcctggag ggcggttaca tcgcctggct gatggatgtg
     1141 atgagcgcgc cggaggagca ggacaaggcg gcagacgtgg agaagagtct ccgcaaaaaa
     1201 ttcaaaaagt cgatctggtc caaatttacg aaagcgatca acacatacga gctggtaaaa
     1261 ccgggagata aaattgctgt ctgtatttcc ggcggaaaag attcgatgtt gatggcgaaa
     1321 tgctttcagg agttaaagct tcacaataaa tttgatttcg aagtaaaatt cgttgtcatg
     1381 gacccgggat acagcccgga gaaccgcaag gtgatcgagg ataacgcaaa gagcctccat
     1441 attccgatca cgattttcga gaccgatatt ttcgattcgg tttaccatgt cgaaaattcg
     1501 ccgtgttacc tctgcgcgag aatgcgccgc ggccatctgt atcattttgc acagcagctc
     1561 ggctgcaaca agattgcact cggccatcat tacgatgatg tcatcgagac gatcctcatg
     1621 ggaatgctct acggtgcgca ggtgcagacg atgatgccga agcttcacag catccacttc
     1681 gagggcatgg agctgatccg cccgctgtat ctggtgcgcg aggatgatat caaggcgtgg
     1741 agagattata acggtctgca tttcatccag tgtgcctgca agtttaccga tacctgcacg
     1801 acctgcaata acgaggagaa tcgttcgaaa cgcgtggaga tcaaggaact gatcaaaaac
     1861 ctgaaaaagg tgaatccgtt cgtggagagc aatattttcc gcagtgtcga aaatgtcaac
     1921 attgacaccg tggtcggcta caagcagcac ggcatccgcc acaatttcct ggaaggatac
     1981 gacgacaatc cggggtatgt gccggaggca gaaaaagcag cggcggaaac ggagcaggaa
     2041 aaagaaggaa agtaatgcac agaaaaatag aagaaacgcc ggggcgggag aaaatgctga
     2101 tccggctgaa agagctccgt ctggaacgcg gttactcgat gacgcaggcg gcgcggaaga
     2161 ttttcagtca cccggacacc tacaaccggt atgaaaaagg cattcgtgag attccgctgg
     2221 attcgctgat gctgctggcg aagacgtatg actgcagtct cgactatctg gcaggtgttt
     2281 cagaccggcc gagaaaatat ggaaggtgat gcagatggca attcctcttt atctgacgac
     2341 gattgaacag gatatttttg atacattccg gtatctgccg catgccgttg cagtcggtgt
     2401 cggctttgtg acggttgcag cagcgtggaa aaaccagcag atgataaaga aacaaaaacg
     2461 gctggagcat gcgcatgcgg ttcctggcac ccagcttctg ctgtggtttc tggtagcagt
     2521 ctattttgcg atgatgcttt ctattacgct gttttccaga gaaccgggtt cgagaacggg
     2581 cgtggatgtg aagctgtttg aaacctgggg aaaccagcgg ctgccggacc ggtattttgt
     2641 ggaaaatatt ctgctgtttc tcccgttcgg cgtattgctg ccggcggcgg ttccgtctct
     2701 tcggaaagga tggtgctgcg tctatgcggc gttttccaca agcatgatgc tggaaacagt
     2761 gcagctctta acagagcgcg gctactgcca gttagatgat gttgtgacga atacgcttgg
     2821 agcagccatc gggtatctgg tgtttgcact ggtgcggaaa tgttggagag ggaagataga
     2881 agaatagaag gaaaacaaga aggctctgcc caaatggaca gagctttttt cttccctgtt
     2941 tttatagcaa gacccgaaaa taatgcaaaa aattcagcac cgcagagcgt gaattactgc
     3001 gtcacagatt ttcgtcgtac cccggtacgc cttcaaatct gttccttgca attcgcgctc
     3061 tgcagcactg ccttttttgc attattttcg ggtcttgcta tagatggaag tgcaggagag
     3121 ttaatccacc cgttctttcc cccagaaaaa caatgccacg gttcccggcc ctgtgtggct
     3181 gccgatggtg gttccgatgt tgttgatctc gacttttccg ttcagattgg gaaagcgttc
     3241 ctcgataaga tccgcaacgg cccgcgcatc ttcgtagcag gcggactggg aaatgtagca
     3301 tttgccggag taggagaggc cgccctcggc gttttcttcc atctggtcga cgattttttt
     3361 gatgactttt ttcttcgtac ggattttgaa ccgcggaatc agccgtcctt cgttgtccat
     3421 gttgagcagc gggcagatgc cgaggaggcc gccgattgcg ccggaggctt tggagatgcg
     3481 gccgccgcgc acgtagaagg tgaggtccgt ggagaagaac cagtgatgga ctttgaggcg
     3541 gtttttcatc gcaaaatcgt aaacctcttc cagcggtttt ccttcatcgc gcaggtcggc
     3601 aaggcggtcc atcagaagac cgtagccgga ggaagcgccg agggaatcca cgatgaggat
     3661 tttccgttcc gggtagcgct cttcaaggtc ggctttggcg atcatcgcgg aattcagtac
     3721 accggaaatg ccggaggaga ggcagacgtg gagaatgtct ttgccgtcct gcaggaatgt
     3781 ttcaaaatag gcttcaaatt catcggcatt cacctgggat gttttcgtgg aagcgccggc
     3841 ggccatggcg gcatagaact gatcgaacgg catggacttg ccgagatcgt ccgcgtactc
     3901 ggtgtcattt aatttgtaat ggaaacagat gtatgagatt tcccgtttgg agaaatgttc
     3961 ttcggtaagg tctgccgtag agcagcagct gatgacatag gaactcataa aataatccct
     4021 cctcgtaaaa atgaaagtaa gaactctggt ttctgtgcct attgtagcat cccgaatggg
     4081 aaaaggcaac tgcggaacgt gattatatcc gtaatgaaag gggaagcgaa atggtgtcaa
     4141 gccttgtatt tgcggtgcgg cctgtggtag aatagaacta tgagaatacg aaaatacaaa
     4201 ggaggttttt gcatatgcag aattattcag gagaagaagg gctgcagtat catctgcaga
     4261 tccgaaaagg cgatgtggga cggtacgtga ttctgccggg agatcccaaa cggtgtaaaa
     4321 aaattgcagc ttactttgag aacccggtgc tcgtcgcaga cagccgggaa tttgtgactt
     4381 atacgggtac tttggacggt gtaaaggttt ccgttacatc taccggcatc ggcggtccgt
     4441 cggcttccat cgcgatggaa gagctgatcc agtgcggcgc cgatacgttt atccgcgtcg
     4501 gaacatgcgg cggtatggca ctcgatgtag agggcggcga tatcgtggtg gcaacgggag
     4561 cgatccgtgc ggagggaacg tcaaaggaat acgcaccgat cgagttcccg gcagtcgcag
     4621 accttaccgt gacgaatgcg ctggtgcagg cggcaaagaa tcttgggaaa aaatggcatg
     4681 cgggcgtggt acagtgcaag gattccttct acggccagca cgacccggag cagatgccgg
     4741 tcggcgatga gctgctgaaa aaatgggatg catggatgaa gcttggatgc aaagcgtcag
     4801 agatggaatc ggcggcactc tttatcgtgg caagtgcccg cggcgtccgt gccggatcgg
     4861 attttcttgt gatgggaaat caggagagag tgaaacgcgg catggagaac cacattacgc
     4921 acgatacgga aggtgcgatt caggtagcga ttgaggcact gcggattctg atccgggaag
     4981 atcagaaata agaaagaaaa aataagggga tactggaaag gggatgctgg atatgaaaca
     5041 ggaaatcagt acagaaataa aacaggagct ggtaaaacag gctctggcag cgcggaaatt
     5101 tgcgtatgtg ccgtattcga aattccgcgt aggcgcggcg gttctggcaa agaatggagc
     5161 gatttatacc ggttgcaaca ttgaaaatgc ggcgtataca ccgacaaact gcgcggagcg
     5221 caccgcggtc ttcaaagccg tatccgaggg cataacggaa tttgacgcga tcgccgtctg
     5281 cggcggcccg ggagaggcag agccggagga tttctgcaca ccgtgcggtg tctgccgtca
     5341 ggtgctgcgg gaattcgttg atccgaacaa atttctcgtc cttctctgca agggcgacgg
     5401 ttcctggaaa gaaatgacac tcgaggagct gctgccaatg ggattcggaa aagaaaattt
     5461 ataaacagaa gccggaaaag aaaaaatcca gggcaggtac ggaaagtacc gtatgccctg
     5521 gattttttat gtccaagata taaaaattat ttactgaaat ttcggctcac aggtcagatc
     5581 gcatcccaga cgctggaaca ttttgcggtc cacatcggag agcataacgc tggagtgcac
     5641 ctggcagccc tttaactgcg gcaggcagga gagtgctttc tgtgcatcct cgctctgtgc
     5701 agcagtagca gacagcgcaa tcagaacctc gtcggtatga agacgcgggt tcttgcttcc
     5761 gaggtatttg gttttcaggg tctggatcgg agcgatggat tccggtgaga gaacatgtac
     5821 gtcatggtcg atgccggaga gctcttttaa tgcattcagc agaagcgccg cgctcgatcc
     5881 gagcagatcg ccggtctttc cggtgatgac gcgtccgtcc ggaagttcca tggcagcggc
     5941 cggtgcgccg gtcgcctcgg cacgaacaag agccggggaa acggtggtac ggtctgcggc
     6001 aacgatgttt tcctgtttca tcagaagctc gatcttgtaa gcctcggtct cggagcggga
     6061 gccgtctgcg ataccgtcca cagcagtgta gtagcggcgg atgatctcct gacgggaagc
     6121 ctcgcggcat gcctcatcat cgaagatgca gagaccggcc atgttgacac ccatatcggt
     6181 cggggatttg tacgggctct cgccgaagat ctgctggaag atcgcacgca gaaccgggaa
     6241 aatctcgaca tcacggttgt agttgacggt tgtcacgccg tatgcctcga ggtggaacgg
     6301 gtcgatcatg ttgacatcgt tcagatcggc ggtagcagcc tcataggcca ggttgaccgg
     6361 gtgcttcagc ggcagattcc agatcgggaa ggtctcatat tttgcatagc cggcgtttac
     6421 accgcgtttg tgctcatggt aaagctggga aaggcaggtt gccatctttc cgcttcccgg
     6481 tcccggtgcg gtaacgacaa cgagcgggcg tgtcgttt
//
LOCUS       DAOMUF010000114         6502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3201_length_6502_cov_3.344036, whole genome shotgun sequence.
ACCESSION   DAOMUF010000114 DAOMUF010000000
VERSION     DAOMUF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3201_length_6502_cov_3.344036"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLN77_07310"
     CDS             <1..332
                     /locus_tag="PLN77_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM44115.1"
                     /translation="MMGKALFTMEVPVTKTTSALTCLTEDEVSALAPSACINCGRCVS
                     VCPGQILPARLSVFAEHGEEEKFLKYGGMECCECGCCSFICPARRPLTQEISSMRKVL
                     LAKRKKK"
     gene            346..1302
                     /locus_tag="PLN77_07315"
     CDS             346..1302
                     /locus_tag="PLN77_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRM44116.1"
                     /translation="MNGLLHVSSSPHARSKVTTDKIMFAVLLALAPAACVGVWNFGLR
                     ALLLIAISMAVCPLTEYLYEKGMKKPVTIADGSALVTGLLLAMNMPVQAPLWMPVIGG
                     VFAILVVKQLFGGLGQNIMNPALAGRCFLLISFPGHMTNFAAPAAAHLVDTVSGATPL
                     AAAKAGEQVNLLSMFLGNTAGTIGETSALALLIGGIFLVCIHVIDLNIPLIYIGTELL
                     FALLFGGHGFDINFLGAHLLGGGLMLGAWFMATDYVTRPITKKGQYIYAVILGLLTGV
                     FRIFGNSAEGVSYAIIFTNLLVPLIERVTVPVGFGRGGKKKA"
     gene            1299..1937
                     /locus_tag="PLN77_07320"
     CDS             1299..1937
                     /locus_tag="PLN77_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM44117.1"
                     /translation="MNKIIKNALILAAITLIAGLLLGAAHQVTLEPIQKQQEKEKQES
                     CQNVFPEAASFEEVTMSDAEQAELTAALIGHGFEAQTIDELLLAKDASGATIGLVEII
                     STTEGYGGGMQFSMGIASDGTTKGISFLSLSETAGLGMRADTDDFKNQFKDKNVEAFS
                     YTKSGASADDEIDALSGATLTTNAVTNGVNAGICCFNYCNESGLLSGEGGNS"
     gene            1934..2638
                     /locus_tag="PLN77_07325"
     CDS             1934..2638
                     /locus_tag="PLN77_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HRM44118.1"
                     /translation="MRKDTPLERLYNGIIKENPTFVMMLGMCPTLAVTTSAKNGIGMG
                     LATMVILTASNLMISLLRKIIPDGVRMPAYIVVVASFVTIVEFLMEGFIPALYSALGI
                     YIPLIVVNCIILGRAEAYAGKHGPIPSLFDGIGMGLGFTVGLTLIGMFRELIGAGTLF
                     GIQVMPSAYEPVNIFIMAPGAFLVLAMLVAVQNRIKRQKSEKTGKPAVPAEETGCMAC
                     ANKAYCQAVKGKEEAK"
     gene            2635..3210
                     /gene="rsxA"
                     /locus_tag="PLN77_07330"
     CDS             2635..3210
                     /gene="rsxA"
                     /locus_tag="PLN77_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HRM44119.1"
                     /translation="MKELILIAIGAALVNNVVLSQFLGICAFLGVSKKMDTAAGMGGA
                     VIFVLTISSFVTSLIYKFILAPAELDYLKTIVFILVIAALVQLVEMFLKKTIPSLYRA
                     LGVYLPLITTNCAVLGVALINVQKDYTILQGTVYGLATAIGFTIAMVLMAGLREKMEY
                     NDIPESFKGMPIVLLTAMLMSMAFMGFSGMI"
     gene            3224..4018
                     /locus_tag="PLN77_07335"
     CDS             3224..4018
                     /locus_tag="PLN77_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HRM44120.1"
                     /translation="MNGILAATLMVGGTGLLIGILLGIAGKKFYVEVDERETKVREAL
                     PGNNCGGCGYPGCDGAAAAVVKGEAPISVCPVGGSPVAAAIGAIMGQEAGAQERRKAV
                     VRCAGTCDVAKNKYEYTGARDCSYAGFVPGGGPKSCGNGCLGFGNCVKACKFDAIHIE
                     NGIAVVDGEACKACGACVKACPRNLIDLIPYEQKITVKCRSTEKGKAVMAACEIGCIG
                     CKKCEKNCPSDAIHVVDQRAEIDPAKCTGCGLCAENCPRKCIMVEK"
     gene            4210..4710
                     /locus_tag="PLN77_07340"
     CDS             4210..4710
                     /locus_tag="PLN77_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HRM44121.1"
                     /translation="MTMTMDELMKVLDMQEDILQFTHFTNADAWELGTMILMEARRRG
                     AQVGIMIRRSNGEVVFQYCDNGITPHNVELMRRKSNSVLLTERSSLQLYMAATNAPES
                     VKQMVLDPKEYAMLGGGFPICVEEVGVVATVAVSGMGQVSDHDVIVKCISKYLHIDEV
                     PRIRTV"
     gene            complement(4824..5714)
                     /locus_tag="PLN77_07345"
     CDS             complement(4824..5714)
                     /locus_tag="PLN77_07345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM44122.1"
                     /translation="MGHRFTYVVSPEEERENLTVEWFLRRHGYSRHIITGLKRTEDGI
                     FRNGVRAWTSQAVHAGDVIETHLVETEPSDGILPRPVPFSVVYEDDDILVVDKPADTP
                     IHPSIGNYENTLANGIAWYFEQKGEPFVYRCINRLDRDTTGLIVLAKHALSAAVLSQA
                     MRSREIHRTYQAFALGETAASGTIDAPIARLNGSLIQRTVDFASGERSVTHYRTLAHC
                     SVFSHLELNLETGRTHQIRVHMAYIGHPLLGDTLYNKTPGTFPRQALHSASLSFVHPI
                     TGEPLNFHAPLPEDMQNLLT"
     gene            5972..>6502
                     /locus_tag="PLN77_07350"
     CDS             5972..>6502
                     /locus_tag="PLN77_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRM44123.1"
                     /translation="MKQVQKPKKPIIFYYVIALIVILLLNLLLFPRLLEPKVTEVDYG
                     KFLQMVDNNEISEVQIQSNEIIFSDKSSPANYYKTGVMNDYKLVDRLYEAGITEFGTP
                     IIEQMSPLMEFLLTWVIPIVVMVALGQWLMKRMTSKMMGGMGNAMSFGKSNAKVYVQS
                     ETGIKFADVAGEDEAKE"
BASE COUNT         1638 a         1455 c         1879 g         1530 t
ORIGIN      
        1 cgatgatggg aaaagccctg tttacaatgg aggttcctgt aaccaagaca acatctgcac
       61 tgacctgcct caccgaggat gaagtatctg cgctggcacc atctgcctgc atcaactgcg
      121 gacgctgcgt ttccgtctgt ccgggtcaga ttcttccggc ccgcctttcc gtttttgcgg
      181 agcacggaga ggaagagaag ttcctgaaat acggcggaat ggagtgctgt gaatgtggct
      241 gttgttcctt tatctgcccg gcaagacgtc cgctgacgca ggaaatcagc tcaatgcgta
      301 aagtgcttct ggcaaagaga aagaagaaat agggggatct tacatatgaa tggattatta
      361 cacgtatcat cttccccaca cgcgcggtcc aaggtgacga cagacaaaat catgtttgcc
      421 gtcctgcttg cccttgcccc ggcagcctgc gtgggagtct ggaatttcgg ccttcgggca
      481 ctgcttttga ttgcgatttc gatggcagtc tgtcccctga cggaatatct gtatgaaaaa
      541 ggaatgaaga aaccggtgac gattgccgac ggcagtgcac tggtaaccgg ccttctgctg
      601 gccatgaaca tgccggtgca ggcgcctctt tggatgccgg tgatcggcgg tgtgtttgca
      661 attctcgtcg taaaacagct tttcggcggt cttggccaga acattatgaa cccggcgctt
      721 gcaggaagat gcttcctttt gatttccttc ccgggtcata tgaccaactt cgctgccccg
      781 gcagccgcac acctcgtgga taccgtatca ggtgcgacac cgctcgccgc agcaaaagca
      841 ggagagcagg tgaatctgct gtccatgttc cttggaaata cggccggaac gatcggtgag
      901 acttccgcac tggcccttct gattggcggc attttccttg tctgcattca tgtgattgac
      961 ctcaatattc cgctcatcta catcggaaca gagcttttgt tcgccctgct tttcggcggc
     1021 cacggttttg atatcaactt ccttggcgcg catcttttgg gcggcggtct gatgctcggc
     1081 gcatggttca tggcaaccga ttacgttacc cgcccgatca caaagaaggg acagtatatc
     1141 tatgcggtca ttttaggtct tctgaccggc gtattccgta tctttggaaa ttcggcagag
     1201 ggcgtttcct atgccatcat ctttaccaac cttctggttc cgctcattga gcgtgtgacc
     1261 gtgccggttg gattcggaag aggaggcaag aaaaaagcat gaacaaaatt ataaaaaatg
     1321 cactgattct tgcggcgatc acgctgattg ccggactgct tctcggggcg gcacatcagg
     1381 taaccttaga gccgatccag aaacagcagg aaaaagaaaa acaggaatcc tgccagaacg
     1441 tattccctga ggcagcttcc tttgaagaag taacgatgag cgatgcagaa caggcagagc
     1501 ttacggcagc actgatcggc cacggttttg aagcacagac gattgacgaa ctgctgcttg
     1561 caaaagacgc atccggcgca acgatcggcc tggttgagat catctccaca accgaaggct
     1621 atggcggcgg catgcagttc tcgatgggaa ttgcatcgga cggaacgaca aaaggaatct
     1681 ctttcctgtc actttccgag acggccggcc ttggtatgcg tgccgacaca gacgatttca
     1741 agaatcagtt caaggataaa aacgtagaag cattctccta cacaaaatcc ggcgcatccg
     1801 cggatgatga aatcgatgcg ctgagcggag caactctgac aacgaacgct gtaacaaacg
     1861 gtgtcaatgc aggaatctgc tgttttaact actgcaacga atccggtctg ctttccggag
     1921 agggagggaa ctcatgagaa aagatacgcc gctggaacgg ctttataatg gtatcataaa
     1981 agaaaacccg accttcgtca tgatgctggg tatgtgtccg acgcttgcgg ttaccacttc
     2041 tgcaaagaac ggtatcggca tgggccttgc gacgatggtt atcctgaccg cctccaacct
     2101 gatgatttcc ctgcttcgga aaatcatccc ggacggcgtg cgtatgccag catacatcgt
     2161 tgttgtggca tcgtttgtaa ccatcgtgga atttttgatg gaaggtttta ttccggcact
     2221 ctattccgcc cttggcatct acatcccgct gatcgttgta aactgtatca tcctgggacg
     2281 cgccgaggca tatgctggaa aacatggtcc gatcccgtct ttatttgatg gtatcggcat
     2341 gggccttgga tttaccgtcg gcctgaccct gatcggtatg tttcgtgagc tgatcggagc
     2401 cggaaccctg ttcggtatcc aggtaatgcc gagtgcttac gagccggtca acatcttcat
     2461 catggcgccg ggtgcattcc ttgtgctggc aatgctcgtt gcggtgcaga accgcatcaa
     2521 acgccagaag tcagagaaaa ccggaaaacc ggcagtcccg gcagaagaga ccggctgtat
     2581 ggcatgcgcc aataaggcat actgccaggc tgtgaaagga aaggaggaag cgaaatgaaa
     2641 gaactgattt taattgcgat cggtgcggcg ctggtgaaca acgttgtctt aagccagttc
     2701 ctcggcatct gtgctttcct cggtgtttcc aaaaagatgg atacggcggc cggcatgggc
     2761 ggagcggtta tttttgtcct cacgatttct tcatttgtga caagtctgat ttacaaattt
     2821 atcctagcac cggcagaact tgattatctg aaaacgatcg tttttattct tgtcatcgcg
     2881 gcgctggtac agctcgttga gatgttcctg aaaaagacga ttccgtctct gtaccgagcg
     2941 ctcggcgttt accttccgct gattacaaca aactgcgcgg tactcggtgt ggcactcatc
     3001 aacgtacaga aggattacac gattctgcag ggtacggttt acggtctggc aaccgcaatt
     3061 ggctttacga ttgccatggt gctgatggcc ggcctgcggg aaaagatgga gtacaacgat
     3121 atcccggagt cattcaaagg aatgccgatc gttcttctta cggctatgct gatgtcaatg
     3181 gcatttatgg gattttccgg aatgatctag gagggatgaa gagatgaatg gtattttagc
     3241 agccaccctg atggtgggtg gaaccggcct tttgattggt attctccttg gaattgccgg
     3301 aaagaagttt tatgtggaag tggacgagcg cgagacgaag gtccgtgaag cgctgccggg
     3361 caacaactgc ggcggctgcg gttacccggg ctgtgacgga gcggcggctg cagtggtaaa
     3421 gggtgaagcc ccgatttcgg tctgtccggt cggcggttct ccggttgcgg cagcgatcgg
     3481 tgccattatg ggacaggaag ccggagcaca ggagcgcaga aaagcagtgg tacggtgcgc
     3541 cggtacgtgt gacgtggcaa agaacaagta tgaatacacg ggtgcaaggg actgctccta
     3601 cgccggtttt gtaccgggcg gcggtccgaa gagctgcgga aacggatgtc ttggttttgg
     3661 aaactgcgtg aaggcatgta agtttgatgc gatccatatt gaaaacggca ttgccgttgt
     3721 ggacggcgaa gcgtgcaagg catgcggtgc ctgcgtgaag gcctgtccga gaaatctgat
     3781 cgacctgatt ccgtatgagc agaagatcac ggtgaaatgc cgctcaaccg agaagggcaa
     3841 ggcggtgatg gcagcgtgtg agatcggctg tatcggatgt aagaagtgcg agaagaactg
     3901 cccgtcggat gcaattcacg ttgtggacca gcgcgcggag atcgacccgg caaaatgtac
     3961 aggctgcggc ctctgcgcgg agaactgccc gcggaagtgt attatggtag aaaaataaaa
     4021 atgagaaatt ccatgctgtg ggagggaact tctgcggcgt ggaatttttg cttttcggtg
     4081 atatcgtgtt agccaatgcc gggggtggct ggcttgactt gaatttggga aaatgataaa
     4141 ctgaaagtca gaaaatgagg ggcgaaaagc taaaaaaata taaaatggca gaaaggacgg
     4201 ggcaaaagta tgacaatgac aatggatgaa ctgatgaaag tgctcgatat gcaggaggat
     4261 attctgcagt ttacgcattt tacaaatgca gacgcatggg aactgggaac gatgatcctg
     4321 atggaggcaa gacggcgcgg agcacaggtt ggcatcatga tccgcagaag caacggggaa
     4381 gttgtattcc agtattgcga taacggcatt acgccgcaca atgtggagct gatgagacgg
     4441 aagagcaatt cggtgcttct gacggagcgg agttcgctgc agctttacat ggcagcgacg
     4501 aatgcgccgg agagcgtaaa acagatggta ctggacccga aggaatacgc aatgctgggc
     4561 ggaggttttc cgatctgcgt ggaagaagtc ggcgttgtgg caaccgtagc agtgtccggc
     4621 atgggacagg tttcggatca tgatgtgatt gtgaaatgca tcagcaaata tttgcatatc
     4681 gatgaagtac caagaatcag aactgtatga aaatgatgat tgaaactaaa ttttgtgtat
     4741 gatacaatgt caccggcaaa aagacatgac agttccatca gggacaacaa aacccccgtg
     4801 agcggcaact cacgggggtt ggtttatgtc agcaggtttt gcatatcttc cggcagtggt
     4861 gcgtggaagt ttaagggttc tccggtgatc ggatggacga aggagagcga tgcagagtga
     4921 agtgcctggc gggggaaagt gccgggagtt ttgttataga gggtatcgcc gagcagcgga
     4981 tggccgatgt aagccatgtg gacgcggatc tggtgggtgc ggccggtttc gaggttgagt
     5041 tcgaggtggg agaaaacgga gcagtgcgcg agggtgcggt aatgggttac ggagcgctcg
     5101 ccggaggcaa agtcgacggt gcgctggatg agggagccgt tgaggcgggc gatcggggcg
     5161 tcgatggtgc cggatgcagc ggtttcgccg agggcgaagg cctgatacgt gcggtggatt
     5221 tcgcgggagc gcatggcctg ggagagaacg gcggcgctta aggcgtgctt ggcgagcacg
     5281 ataaggccgg tggtgtcgcg gtcgaggcgg ttgatacagc ggtagacgaa gggttcacct
     5341 ttctgttcaa aataccatgc aatgccgttt gccagcgtat tttcataatt tccaatcgac
     5401 ggatgaatcg gcgtatcagc gggtttatcc acaacgagga tgtcgtcatc ctcatacaca
     5461 accgaaaacg gaaccggccg cggcagaatt ccgtcggagg gttctgtttc gacaaggtgc
     5521 gtttcgatca catccccggc gtgcacggcc tggctcgtcc aggcgcggac gccgtttcgg
     5581 aaaatgccgt cctctgtgcg ttttaagccc gtaataatat ggcgcgaata tccatgccgg
     5641 cggagaaacc attccacagt cagattttcc cgttcctcct ccggcgaaac cacatatgta
     5701 aacctgtgtc ccatacacaa aacctccgtt tctatagctt ttttatcgaa tgaaccccgt
     5761 catttcgggg caaaatttca aaaagagact ttcccagtct ttcgcaagta tagccgaaaa
     5821 cagatggaaa agcaagaaac ttaccttttt atagcgaaaa tataaaaaag tataaaagtt
     5881 tcatgaaatg tcagaatttg attgatttta gcgaaagaaa agtttatgct actaaaaaag
     5941 aaagaaaatc aagaaacagg aggggataca gatgaagcag gtgcaaaaac cgaaaaaacc
     6001 cataattttt tattatgtga ttgcgctgat cgtcattctg ctgttaaatc ttctgctttt
     6061 cccgagactt ctcgagccaa aggtaacaga agttgattac ggaaaatttt tgcagatggt
     6121 agataacaac gaaatcagcg aagtccagat ccagtcaaat gagattattt tcagcgacaa
     6181 atcaagcccg gcgaattatt ataagacggg tgtgatgaat gattacaagc tggtagaccg
     6241 tttatatgag gcaggtatta ccgaatttgg aacgccgatc atagagcaga tgtcgccact
     6301 catggaattt cttttaacat gggtaatacc gatcgttgtg atggttgcgc tcggacagtg
     6361 gctgatgaaa cgcatgacga gtaagatgat gggcggcatg ggaaatgcga tgagctttgg
     6421 aaagagcaac gccaaggttt atgtacagtc cgaaaccggc attaaatttg cagatgttgc
     6481 cggagaggat gaggcaaaag aa
//
LOCUS       DAOMUF010000115         6500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3204_length_6500_cov_2.732506, whole genome shotgun sequence.
ACCESSION   DAOMUF010000115 DAOMUF010000000
VERSION     DAOMUF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3204_length_6500_cov_2.732506"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /gene="aspS"
                     /locus_tag="PLN77_07355"
     CDS             <1..1053
                     /gene="aspS"
                     /locus_tag="PLN77_07355"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HRM44124.1"
                     /translation="DLHLLEVEILSEPEEAPPVVINQKRVNTSIENLLDYRPITLRNE
                     KERAVFQIQARICQAFREFLDGQHFTEIHTPKLVAAGAEGGANIFSLSYFGEQAYLAQ
                     SPQFYKQMMVGVFERVYEIAPVFRAEKHDTARHLNEYTSVDFEMGYIENFEDIMAMEQ
                     EMLRYTFARLQETCGAELSLLKAEVPVITEIPRIRFSEAKELVSRVYKRAFSEKLDFE
                     PEEEKLLCEYVKKTTGSDFVFVTHYPSAKRPFYAMDSRENPAVTESFDLLFRGLEVTT
                     GGQRIHNYREQVEKIESRGMHVEAFESYLMMHRCGMPPHGGLGLGLERFTARLLGFEN
                     VRNASLFPRDIHRLIP"
     gene            1377..1835
                     /locus_tag="PLN77_07360"
     CDS             1377..1835
                     /locus_tag="PLN77_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44125.1"
                     /translation="MKLYDISGEQFSAMDAPRHLMTEEGTAQTTLDRSLGICQVAELE
                     GKVTREVLEEVMEEDCERLLIRGDAQITRDGADYLAEKKLVLLGVEQETVGNETSGND
                     VHQQLLKKGTVIVENLNLEKVTSGHYFLVAAPVKKKHQYGGPIRAVLIEN"
     gene            2066..3523
                     /locus_tag="PLN77_07365"
     CDS             2066..3523
                     /locus_tag="PLN77_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM44126.1"
                     /translation="MDAKMKVERAAAGAAIDGVLKYVNHGDDRTKALLNLTDFVQKFT
                     GNMFAEKTFDNIRTMLQNPDNKWVQYVNRGLDELDPQVIKMTALNLGFQAAFVGTKQI
                     RMNREKYNCNIPWTMLMDPTSACNLHCTGCWAAEYGHKLNLSYEKLSDIISQGKELGT
                     YFYMFTGGEPLVRKKDILRLAEEHHDCEFHCFTNGTLIDEEFCEAVQKLGNISFSLSL
                     EGFEEVNDGRRGEGIFDKVLTAMDLMKKHGLLFGTSICYTRANLETVTSDEFLDLLIE
                     HGCRYSWYFHFMPVGMDAAPELMPTVEQRKYIYHRLREIRSDKSDKQIFVMDFQNDGE
                     FVGGCIAGGRNYCHINANGDVEPCVFIHYSGANINEVSLIEALQQPLFQAYRDGQPFN
                     RNHLRPCPMLENPELLREMVEKTGAKSTDLQSPESAEHLCGKCDLYAAEWKKAADELW
                     AENPHHKQRYENYKKENLDEEAKASTEEILSGIRG"
     gene            3709..>4149
                     /locus_tag="PLN77_07370"
     CDS             3709..>4149
                     /locus_tag="PLN77_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM44127.1"
                     /translation="MNDSFLQEQGVSGALIESVRRFRESYPVAEGLENRMSTPEIPFF
                     GKEILEMAIAALLQGENLLLTGEKATGKNVLAETLAYVFGRPTYNVSFHVNTGSAELI
                     GTDTFQNQEVTLRKGSIYRCAEEGGFGILDEINMAKNDAVSVLHA"
     assembly_gap    4150..4159
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4160..>4612
                     /locus_tag="PLN77_07375"
     CDS             <4160..>4612
                     /locus_tag="PLN77_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44128.1"
                     /translation="RRIIDVPGYDKIPLHPAARMIGTMNYGYAGTRELNEALVSRFLV
                     IDMPPLTEETLFYLMQSKFPDLKPAARDALAGLYLDLQKKAKQAEITTRALDLRGLFG
                     LALAMTVVCLGQTVAIAKAVAARPRAIWPRPSTPTWVAWTLSRTPSPK"
     assembly_gap    4613..4622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4623..4782
                     /locus_tag="PLN77_07380"
     CDS             <4623..4782
                     /locus_tag="PLN77_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRM44129.1"
                     /translation="FGAIGTMRQGLSPYLAVQIGIVNKCFDLFEKEIVRDVVRTRIPE
                     EWTPQDVF"
     gene            4794..>6500
                     /locus_tag="PLN77_07385"
     CDS             4794..>6500
                     /locus_tag="PLN77_07385"
                     /inference="COORDINATES: protein motif:HMM:NF023203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein"
                     /protein_id="HRM44130.1"
                     /translation="MVTEETLKTHEMELESRIRNLVWTVSGDYTLKLNPDVERYAKEP
                     DTVLYDTIRQGAFSCYFDREAYSLYLVKKVYSGAAEGELMMLAQLVTEEAVSKRLESE
                     RPGVKGFRRRAAEEILDHSFETLSRNETGRLKLEYLRGRREEDEGKSPSYSGKTREWM
                     ELLKHASETADTMELIRVTDELYNKVVDPAFVKKHGDLASVLAVTIEELTEYSWKDFL
                     SEELYEDGLETYLERVSLDMTNFTSKEAEEKKEKTAGSDTKKVVVVDEKALARMYQYI
                     QRNYGKTWLSESEEKSRNYQLCRGIHSDCSLYYTEGVLKNPVGKYYQLSYAQKQKDKN
                     LHAYYDQHRAVRQNISLLYQELKKAMLLQEDQSFADADHGILQPARMWKVGRSQNADL
                     FRLEQRRNSLDFVVDILVDASGSQRPRQENVTLQTYILAETLSRLHIPFRVMSFCTFW
                     DYTILHRMRDYDDPREKNSNIFEYITSSNNRDGLAVKAAGIDLLKRQEDRKLLILLSD
                     GKPYDVLVNRPNARNPKPYRDDYALHDTATEVRRLRQQGVSVLGVFVGEETELVAEQK
                     IFG"
BASE COUNT         1761 a         1491 c         1892 g         1336 t
ORIGIN      
        1 gacctgcatc tgttagaagt ggaaatcctc tccgagccgg aagaagcacc gccggttgtc
       61 atcaaccaga agcgggtcaa cacttccatc gagaacctgc tggactaccg gcccatcacg
      121 ctgcgaaatg aaaaagagcg cgcggttttc cagattcagg ccaggatctg tcaggcgttc
      181 cgcgaatttc tggatggaca gcacttcacc gaaatccaca caccgaagct cgtagcggca
      241 ggagccgagg gcggagctaa tatcttctct ttgtcgtatt tcggcgaaca ggcatatctg
      301 gcgcagagcc cgcagtttta caagcagatg atggtcggcg tcttcgagcg tgtctacgag
      361 atcgcaccgg tcttccgggc ggagaaacac gacacggcgc gtcatctgaa cgaatataca
      421 agcgtggact ttgagatggg ctatatcgag aacttcgagg acatcatggc gatggaacag
      481 gagatgctgc ggtacacgtt tgcacggctg caggagacat gcggagcgga gctttcgctt
      541 ttaaaagcag aggtgccggt gatcacggag attccgagaa tccgtttttc cgaggcgaaa
      601 gagctggtga gccgcgtata caaaagagcc ttttcggaga aactggactt tgaaccggag
      661 gaggaaaaac tgctgtgcga atacgtgaag aaaacgacag gcagtgactt cgtatttgtg
      721 acacattatc caagcgccaa gcgcccgttc tacgcgatgg acagcagaga aaacccggca
      781 gtgacggaaa gttttgacct tctcttccgc ggccttgaag tgacgaccgg cggccagcgc
      841 atccacaatt accgggagca ggtggaaaag atcgaaagcc gcggcatgca tgtggaagcg
      901 tttgagagct atctaatgat gcaccgctgc ggcatgcctc cgcacggcgg cctcggtctt
      961 gggctggagc ggtttacggc acggcttctc ggatttgaaa atgtgcggaa cgcatccctg
     1021 tttccaagag acattcaccg tctgattccg taaaccagaa gagtgtaaaa aactccggcg
     1081 aagccgtggc gcagatccac gggcttagcc ggagtttcat aagatttccc tcccttttga
     1141 aagagagcgt aaccgtttgg atatttctat tatacaccag gggagtgaaa atgacaaatt
     1201 gtttattgca gacaggattg ctgtataaaa gtcagaattt ttgcagaaat gagccttgta
     1261 aagcgtccga aaataggcta tactaaacaa ggtttggctg ccggcagaat ctggcagtat
     1321 cggcaaatat tggaaaaaag aaagacggga gtttcccggt ttgaggatag gaaaatatga
     1381 aattatatga catttccgga gagcagtttt ccgcaatgga cgcgccgcgt catctgatga
     1441 cggaggaagg aacggcgcag acgacgctcg accggtcact tggcatctgt caggtggcgg
     1501 agcttgaggg aaaagtgaca agagaagtgc tcgaggaggt catggaagaa gactgtgaac
     1561 gtcttctgat ccgcggcgat gcgcagatta cacgcgatgg ggcagattac ctggcagaga
     1621 aaaaattggt gctgctcggt gtggagcagg aaactgtcgg aaatgagaca tcggggaatg
     1681 atgttcatca gcagctgctg aaaaaaggta cggtgattgt cgaaaatctg aatctggaga
     1741 aagtgacctc tggtcattat tttcttgtgg ctgctccggt caaaaagaaa caccagtacg
     1801 gcggaccgat ccgcgcggtt ctgatcgaaa attagaaaat gctttgtgtg aatctgaaaa
     1861 tgcaatgtaa aatataacgg aaagagaaaa acaggaaaaa gtgttccctt gtgggacact
     1921 ttttttgatg tgtccaaaac gagggaattt tgggttgtat tttccaaata tggagaatat
     1981 aatgggaaaa agccaggatg ttgtattcaa aaccagatca attccgggcg aaaatattaa
     2041 actattacaa aggagatcgt gaacaatgga tgcaaaaatg aaagtagaaa gagccgctgc
     2101 aggcgctgcg atcgatggtg ttttaaaata tgtgaatcac ggagacgaca gaacaaaagc
     2161 gctgttgaat ctgacggatt ttgtacagaa atttaccgga aatatgtttg cggagaaaac
     2221 cttcgacaac atccgcacca tgctgcagaa tccggacaac aaatgggtac agtatgtcaa
     2281 ccgcggactg gacgagctgg atccgcaggt gatcaaaatg actgctttga acctgggatt
     2341 ccaggcagct ttcgtcggaa cgaaacagat ccgtatgaac cgtgaaaaat acaactgcaa
     2401 catcccatgg acgatgctga tggacccgac aagtgcctgt aacctgcact gcaccggatg
     2461 ctgggcagca gaatacggcc acaagctgaa tctttcctat gagaaacttt ctgatatcat
     2521 aagccaggga aaagagctgg gaacctactt ctacatgttc accggcggcg aaccgcttgt
     2581 gcgcaaaaaa gacatcctgc gtctggcaga agagcatcat gactgtgaat tccactgctt
     2641 caccaatgga accctgatcg acgaggagtt ctgtgaggcg gttcagaagc tcggcaatat
     2701 ctccttctcc ctgtctctgg aaggcttcga ggaggtcaac gacggacgcc gcggcgaggg
     2761 tattttcgat aaagttctga cagccatgga tctgatgaaa aaacacggtc tgctttttgg
     2821 tacttccatc tgctacacaa gagcaaacct cgagaccgtt acaagtgacg agttcctgga
     2881 tcttttgatc gagcacggat gccgttacag ctggtatttc cacttcatgc cggtcggcat
     2941 ggacgctgca ccggagctga tgccgacggt tgagcagaga aaatatatct atcacagact
     3001 gcgtgagatc cgttccgata agagcgacaa acagatcttt gttatggact tccagaacga
     3061 cggtgagttc gtcggaggct gtatcgcagg aggaagaaac tactgccata tcaatgcaaa
     3121 cggcgatgtg gagccgtgtg ttttcatcca ttactccggc gccaacatca acgaagtttc
     3181 cctgatcgaa gcactgcagc agccgctgtt ccaggcatac cgcgatggac agccgttcaa
     3241 ccgcaaccac ctgcgcccat gtccgatgct cgaaaatccg gaactgctgc gtgagatggt
     3301 agagaagacc ggcgcgaagt caaccgacct gcagtctccg gagagcgcag agcatctgtg
     3361 cggaaaatgt gatctgtatg ctgctgagtg gaaaaaagcc gcagacgagc tgtgggcaga
     3421 gaatccgcat cacaaacaga gatatgagaa ctataagaaa gagaacctgg acgaggaggc
     3481 aaaagcttcc acagaggaaa ttctttccgg aatccgcgga taatgcgatc gatgaatcga
     3541 agataaaata gaagcaagac accggaaaat gggacgaaaa tctgttttcc ggtgtctttt
     3601 tgtgcgggca gtgagccaaa gtccgacctt gcagaaagcg gccggatccc gtatactata
     3661 gagaagcaaa gcgatatttt aacgacagag aggattaaaa aatagaagat gaacgattca
     3721 tttttacagg aacagggcgt aagcggggcg ttaattgaga gcgtgcggag attccgcgag
     3781 agctatccgg tggcggaggg cttagagaac cgcatgagca cgccggaaat cccgtttttc
     3841 ggaaaagaga ttctggagat ggcaattgcc gcgctgctgc agggcgaaaa tctgctgctg
     3901 accggcgaga aggcaacggg aaaaaacgtg ctggcagaga cactggcgta cgttttcgga
     3961 agaccgacgt acaacgtttc tttccatgta aacacaggaa gcgccgagct gatcggaacc
     4021 gatacgttcc agaaccagga ggtcacactc cggaaaggca gcatttaccg ctgtgcagag
     4081 gaaggcggtt ttggcattct cgatgagatc aacatggcga aaaacgatgc ggtcagtgtt
     4141 cttcacgccn nnnnnnnnna ccgccgcatc atcgatgtgc cgggctacga taaaattccg
     4201 cttcacccgg cggcgcggat gatcggaacg atgaattacg gctatgcggg aacgagggag
     4261 ctgaatgaag cgctggtgtc ccgctttctg gtgattgaca tgccgccgct tacagaagag
     4321 acactgtttt atctgatgca gtcaaaattc ccggatctga aaccggcggc acgcgatgcg
     4381 ctggccggtc tttacctcga tctgcagaaa aaggcaaaac aggcggaaat caccacgcgc
     4441 gcgctcgacc tccgcggcct gttcggcctc gcgctcgcca tgacggtcgt gtgcctcggg
     4501 cagacggtcg ccatcgccaa ggcggtggcg gcgcgcccac gggcgatttg gccaaggcca
     4561 tcgacgccga cctgggtggc ctggacgctt tcaaggacgc cttcaccaaa gcnnnnnnnn
     4621 nngttcggag cgatcggcac gatgcgtcag gggctgtccc cgtatcttgc ggtgcagatc
     4681 ggcattgtca ataaatgctt tgacctgttt gaaaaagaga tcgtgcggga tgttgtgcgc
     4741 acgcggattc cggaagagtg gacgccgcag gatgtttttt agaggaaata cgtatggtga
     4801 cagaggaaac attaaaaacg catgagatgg agctggaaag ccggatccgc aatctcgtct
     4861 ggactgtcag cggcgactac acgctgaagc tgaacccgga cgtggagcgg tatgcgaaag
     4921 agccggatac ggtgctgtac gatacgatcc gccagggtgc cttttcgtgt tactttgatc
     4981 gggaagccta cagcctgtat ctggtgaaga aggtttacag cggcgcagcg gagggagaac
     5041 tgatgatgct cgcccagctg gtgacggagg aggccgtatc gaagcggctc gaaagcgagc
     5101 gccccggtgt caagggcttt cgaaggagag cagcggagga aatactggac cactcttttg
     5161 aaactctatc cagaaatgag acaggccgcc tgaaactgga gtatctgcgc ggaagaagag
     5221 aggaggacga gggaaaaagc ccttcatact cgggaaaaac gcgggagtgg atggagctgc
     5281 tgaagcacgc atccgaaaca gcggatacaa tggaactaat tcgggtgacg gacgagctgt
     5341 acaataaggt cgtcgatccc gcatttgtga aaaagcacgg tgatcttgcc tcggttcttg
     5401 cggtaacgat cgaggagctc accgagtatt cctggaagga tttcctttcg gaagagctgt
     5461 atgaggacgg gctcgagaca tatctggagc gagtttcgct tgatatgaca aatttcacat
     5521 caaaagaggc ggaagagaag aaagaaaaaa cagcgggaag cgataccaaa aaggtcgttg
     5581 tcgtcgatga gaaagcgctg gcacggatgt atcagtacat tcagcgcaat tacggaaaaa
     5641 cgtggctctc ggaaagtgag gagaaaagcc ggaattacca gctctgccgc ggcatccaca
     5701 gcgactgcag cctctattac acggagggtg tgctgaaaaa tccggtcgga aagtattatc
     5761 agctctccta cgcgcagaag cagaaggata aaaaccttca tgcctattac gaccagcacc
     5821 gcgcggtgcg gcagaacatt tcgctgctgt atcaggaact gaaaaaagcg atgctgctgc
     5881 aggaggatca gtcgtttgcg gatgcagacc acggaatttt acagcccgcg cggatgtgga
     5941 aggtgggacg gagtcagaat gcggatctgt tccggctgga gcagcggcgg aattcgctgg
     6001 attttgtcgt ggatattctc gtggatgcga gcggttccca gcggccgcgc caggagaatg
     6061 tgacactgca gacgtacatt ctggcggaga ccttaagccg cctgcacatc ccgttccgcg
     6121 tgatgagctt ctgtacgttc tgggattaca cgatcctgca ccggatgcgc gattacgacg
     6181 atccgcggga gaagaacagc aatatttttg agtatattac atcatcaaac aatcgcgacg
     6241 ggcttgctgt caaggctgcg gggatagatc ttttaaaacg ccaggaggac cggaagctgc
     6301 tgatcctctt aagcgacgga aagccgtacg atgtgctggt gaaccggccg aatgcgagaa
     6361 atccaaagcc gtaccgggat gattatgcgc tgcacgacac ggcaaccgag gtgcggcgcc
     6421 tcaggcagca gggcgtttcg gtgctcggag tgttcgtagg tgaggagacg gaacttgtgg
     6481 cggagcagaa gattttcggc
//
LOCUS       DAOMUF010000116         6463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3229_length_6463_cov_2.730805, whole genome shotgun sequence.
ACCESSION   DAOMUF010000116 DAOMUF010000000
VERSION     DAOMUF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3229_length_6463_cov_2.730805"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLN77_07390"
     CDS             complement(<1..1080)
                     /locus_tag="PLN77_07390"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRM44131.1"
                     /translation="MEFQKGMDLSFIPELEDAGVQVKDFDGTVMDPLDLVQKYGVNSV
                     RLRIWNAPEHVRESGGYCSYAHTLAMAKRIRAHGMSFMLDFHYSDFWADPGQQRKPKD
                     WENLSFKELKEAVFSYTRDTLTALKEEDVLPDIVQIGNEIRSGLLFPDGELPDYTKMV
                     QLVNAGIRGARAAAGKDEMQVMIHLDQGGRYFYLKEWFDCSFEAGLEDFDLIGLSYYP
                     FWHGTFTDLKETMTKLIQDCKKPITVDETAHAWRKSAHGFIDEQQEKIAGFPATPAGQ
                     KQVLDLVANIVASLPDEMGQGIYYWEPICVPKDGEGGWSENMGLLEEKGTIMEGIRSF
                     LFTRNDACPEKPAKIYEPEPMTVVKD"
     gene            complement(1102..2076)
                     /locus_tag="PLN77_07395"
     CDS             complement(1102..2076)
                     /locus_tag="PLN77_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM44132.1"
                     /translation="MTLEEIAKEIGVSKSTVSRALSGKGRIGEKTRNRILEVAKRGEE
                     REEMARTRTVTHNIGVVLPSDVYYGSGVYFQNCLLGVCESASMLGYNVLITTSNAHDI
                     SELQSLVESNRVDGMLLTRALEDDRALQYLSQANFPTALTGTCRYDNIIQVDTDNEGA
                     AEKMTTLLIGKGFRRFAFLVKDMSYNVDRKRHEGFCRALIKNGISEKDQLFYNGSIRM
                     EFLDAIIENIISKKVECIICGDDEICTMVMSRLQAEGYRIPKDVAIASLYNSTNLDCY
                     SPAVTAVSVAASQMGGTAVRQLIQRLRGENYEKKTMLDYDILFRKSTN"
     gene            complement(2265..3848)
                     /locus_tag="PLN77_07400"
     CDS             complement(2265..3848)
                     /locus_tag="PLN77_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM44133.1"
                     /translation="MKKSTKKLVSVLLASAMAAGMLAGCGSGNGKGSTGDSKSSSAAS
                     GTSGDNLSADTSEHVDLTMYLIGDRTPDFDEVYAKINEILEEKLNCSLNVEFLSWGEH
                     DTKYSLLFSSQEDFDLIFTASSWCHYEQTVSLGGFAPLSEDFIKTYAPDIWEVVPEMA
                     WEQAKIDGQIYMVPSYANEFGQEVLAVRGDLMEKYGMDDITSWDDLMKFYKACAADGI
                     YASQGGPWYPFFQSQGYSTTGGAPKGGELILYHTQDPKDLEFQYIGEWDAFREYCQEM
                     KDLVDAGAWSSDVLNSSDERQTGLLTGRTASMSWNLGTCLTYGKQANEEHPDWNVTMV
                     DPNKNLSKKVNSYINNGVAINANSKNKERAMMVLNEFYTNPDVYDLAMLGIEGKHWEA
                     VGDDQYKVIDESGYGVASNCNWGWNNCTIQREEYLENRTALDDKYESIKDAFNNNIKE
                     DHVYDGFNFDSSNVSTQFAAVEAAIDNYYNPLINGLVDDVDASIDAMNAAMDSAGIQD
                     ILDEMNRQAAEYVAEKEEK"
     gene            complement(4059..4979)
                     /locus_tag="PLN77_07405"
     CDS             complement(4059..4979)
                     /locus_tag="PLN77_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM44134.1"
                     /translation="MGSSSHTSKIKRGKDAVALDIIAYGFCGIIALVCLVPFIMIVSG
                     SFSSESAIIQNGFSLLPQDFSLEAYKTVFKDPMVVFRAYGTTIGLTIVGTVLGLLLQT
                     MTAYVLSRKEFEWRNQFSFFFYFTTLFSGGLVPYYVLMTQTLNLRDSYLALLLPLLFS
                     VYNLLIMKSYIVGLPDSLIEAAKIDGCGEFRILFRIVMPLIKPALATVGLFIALAYWN
                     DWYNAMLYIKDTNKYPLQYFLYQQVNDIEAYKKLLANNAAGSAVSAISLPTQSLKMAL
                     TIVVTGPIVLAYPLVQKYFVQGITIGAVKG"
     gene            complement(4992..5921)
                     /locus_tag="PLN77_07410"
     CDS             complement(4992..5921)
                     /locus_tag="PLN77_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM44135.1"
                     /translation="MKKNGWKGFCKKLYKYRVFLLMLAPAVIYTLIFSYYPMTGIVMA
                     FKKYSYAGGIWGSPWNGFDNFKFFFKSGQAGLVTRNTVLYNLAFIAINTTMQIAVAVL
                     LTEIRNKHYRKITQSLMFLPYFISWVIVGVMAFNIFSSDYGFLNRLIETFGGTPIKFY
                     TTPKAWPFILVFFNVWKGIGYGSVMYLAAIMGIDTSIYEAARIDGANVFQRIFKVTIP
                     MIMPTTIILLLLSVGGIFKGNFDMFYNLVGSNGLLYNYTDVIDTLTFRALISSNDFGM
                     SAAVGLYQSVLCFITVVIVNKLVGLYDKDYTLF"
     gene            complement(6312..>6463)
                     /locus_tag="PLN77_07415"
     CDS             complement(6312..>6463)
                     /locus_tag="PLN77_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRM44136.1"
                     /translation="IFAGPDEVVTLSHRAYSKGVFGKGAVEAAKFLAGKPAGMYDMQD
                     VIAAK"
BASE COUNT         1567 a         1679 c         1370 g         1847 t
ORIGIN      
        1 atcttttact acggtcattg gctccggctc gtaaatcttg gccggttttt ccggacaggc
       61 atcattccgt gtaaacagga aagaacgaat gccctccatg atggttccct tctcctccag
      121 cagacccata ttttccgacc agccgccctc accgtctttc ggaacacaga tcggttccca
      181 gtaatagata ccctgcccca tttcatccgg cagcgaagca acgatattgg caaccagatc
      241 cagcacctgc ttctgtccgg caggcgtcgc cggaaatcct gcgatttttt cctgctgttc
      301 atcgataaac ccatgcgcac tctttctcca tgcatgcgcc gtctcatcga ccgtaatcgg
      361 ctttttgcaa tcctggatca gcttcgtcat tgtctctttc agatccgtga aagtgccgtg
      421 ccagaatgga taataggaaa gtccgatcag gtcaaaatcc tcgagtcctg cctcaaagga
      481 acagtcaaac cactctttca gatagaaata ccgtccgccc tggtcgagat ggatcataac
      541 ctgcatctca tcttttccgg ctgcagcccg cgcaccgcgg attccggcat ttacaagctg
      601 taccattttt gtataatccg gcagctctcc gtccggaaac agaaggccgc ttcggatctc
      661 atttccgatc tgaacaatat ccggcagaac gtcctcctct tttaatgccg tcagcgtatc
      721 tcttgtataa gaaaaaacag cctcttttaa ttctttaaag cttaagtttt cccaatcttt
      781 cggctttctc tgctgaccgg gatccgccca gaaatcagaa taatggaaat ccagcataaa
      841 gctcatgcca tgtgcccgga tccgttttgc catcgccagt gtatgcgcat agctgcagta
      901 tccgccggac tccctgacat gctccggcgc attccaaata cggagccgta cggaatttac
      961 cccgtatttc tgaacgagat ccagcggatc cataacagtt ccgtcaaagt ccttcacctg
     1021 tactccggca tcttccagct ccggtataaa agatagatcc attcccttct gaaattccat
     1081 tgttctgtcc tccctgctac gttagtttgt cgattttcgg aataaaatgt cgtaatccag
     1141 catcgtcttc ttctcataat tttcccctcg gagccgctgg atcagctgac gcacggctgt
     1201 gcctcccatc tgggatgctg ccacactgac tgctgtaact gcaggggaat agcagtccag
     1261 attggtgctg ttgtagagcg aagcaatcgc gacgtccttg ggaattcgat agccttccgc
     1321 ctggagccgg gacatcacca tcgtacaaat ctcgtcgtcg ccacagatga tgcattccac
     1381 ttttttggat ataatatttt caatgatcgc atccagaaac tccatacgga tcgaaccatt
     1441 gtagaaaagc tgatccttct cggaaattcc atttttaatc agcgcccggc agaaaccttc
     1501 gtgacgcttg cggtctacgt tatagctcat atccttgaca agaaacgcaa atctgcggaa
     1561 tccctttccg atcagcagcg ttgtcatttt ctctgccgca ccctcattgt cggtatccac
     1621 ctggataatg ttgtcatacc ggcatgttcc cgtcagtgct gtcgggaaat ttgcctgact
     1681 taaatactgc agcgcacggt catcctcaag cgcacgtgtc aggagcatac catccacccg
     1741 gttcgactcc accagcgact gcagctctga aatatcatgc gcgttgctcg tcgtaatcag
     1801 cacattgtac ccgagcatcg atgccgattc gcagacgccg agcagacagt tctggaaata
     1861 aacaccgctt ccataataaa cgtctgaagg cagtaccacg ccgatgttgt gcgtcaccgt
     1921 tcgggtccgc gccatctcct cccgctcttc tccccgtttt gccacctcga gaatccggtt
     1981 tctggtcttc tctccgattc tgcccttacc ggacagtgca cgggaaacgg tactttttga
     2041 tacccctatc tcttttgcaa tttcctccag tgtcataaaa ataccctgtc actttctgtt
     2101 aaatttgaaa aattcctata tttttatatt taatacgaat gctgttagaa atcaagtcat
     2161 ccctcaaagc tttttgaacc atcacttgat ctctaaaaga aggaacggcc gcgccggagc
     2221 ttctggcaga ccagcgcgtc cattccctgt tttattttac tgttttattt ttcttctttc
     2281 tccgctacat actctgctgc ctgacggttc atctcatcca gaatatcctg gattccagcg
     2341 ctgtccatag ctgcgttcat ggcatcgatg gaagcgtcca catcgtcaac gagaccgttg
     2401 atcagcgggt tataataatt gtcgatggct gcctctactg ctgcaaactg tgtacttaca
     2461 ttgctggaat caaagttaaa tccgtcgtaa acatgatctt ctttgatgtt gttgttgaag
     2521 gcatctttga tgctttcata cttgtcgtcc agagccgttc tgttctcaag atattcctct
     2581 ctctggatgg tgcagttgtt ccatccccag ttgcagttac ttgctacacc atagccgctc
     2641 tcatcaataa ctttgtactg atcatcaccg actgcttccc agtgctttcc ttcgataccg
     2701 agcatagcca gatcgtaaac atccgggttg gtgtagaatt catttaatac catcatggct
     2761 ctctctttgt ttttactgtt tgcgttgatg gcaacaccgt tgttgatgta agagtttact
     2821 tttttcgaca ggtttttgtt cgggtctacc atcgttacat tccaatccgg atgttcctca
     2881 tttgcctgtt ttccgtaggt aagacaggtt ccgagattcc agctcatgct tgctgttctt
     2941 ccggtcagaa gtccggtctg tctctcatcg ctgctgttta atacgtcgct ggaccatgct
     3001 ccggcgtcta ccaggtcttt catctcctgg cagtactcac ggaacgcatc ccattcaccg
     3061 atgtactgga attcaagatc tttcggatcc tgggtatgat acagaatcag ctcgccgccc
     3121 ttcggtgctc cgcccgttgt ggaatagccc tgggactgga agaatggata ccacggtccg
     3181 ccctgacttg catagatacc gtctgctgca catgctttgt agaacttcat caggtcatcc
     3241 caggatgtaa tatcatccat accgtatttt tccatcaggt caccgcgcac tgccagaact
     3301 tcctgaccaa actcgttggc atagctcgga accatgtaga tttgtccgtc aatctttgcc
     3361 tgctcccatg ccatttccgg aacaacctcc cagatatccg gtgcatacgt tttgataaag
     3421 tcctcagaaa gcggtgcaaa tccgccgagg gaaacggtct gctcgtaatg gcaccagctc
     3481 gatgcggtaa agatcaggtc aaaatcttcc tgtgaggaga acagcaggga atatttcgtg
     3541 tcatgctcgc cccaggaaag gaattctacg ttcagagagc agttcagctt ctcttccaga
     3601 atctcattga ttttggcgta aacctcatca aaatccgggg tacggtcgcc gatcagatac
     3661 atcgtcagat cgacatgctc cgaagtgtct gcactcaggt tgtctcccga tgtgccggaa
     3721 gctgcggagc tgcttttgct gtcgcccgtg cttccctttc cgttgccgga accgcatcct
     3781 gccagcatgc cggctgccat tgcggatgca agtaatacag ataccagttt ttttgtgctc
     3841 tttttcataa agaacctcct tcataaatat aatgtgtaat tcaagcggat attcgcaatc
     3901 cgctttgcta cttgctcata accgaataac tgctccgcag tttatcagaa ggagcttgca
     3961 ctcctcctga tacaagcaag tccccaccca cctgcttatt gcttttcgca atggaaacag
     4021 gggacttact tgattcagtt aaattaacat gatttgtatc agcctttgac tgctccgatt
     4081 gtgataccct gtacaaagta tttctgtacc agcggatatg ccagaacgat cgggccggtt
     4141 actacaatgg taagtgccat tttcagggac tgggtcggca atgagattgc gctgacggca
     4201 gagcctgcgg cgttgtttgc aagaagcttt ttatatgcct cgatatcatt aacctgctga
     4261 tacaggaaat actgcagcgg atacttgttc gtgtctttga tatacagcat cgcattgtac
     4321 cagtcattcc aataagccag tgcaatgaag aggcccacgg tagcaagtgc cggtttgatc
     4381 agcggcatta caatccggaa cagaatacgg aactcgccgc agccatcaat cttggccgct
     4441 tcaatcagag aatccggaag tcccacgatg taactcttca tgatcagaag gttgtatacg
     4501 ctgaacagca gcggaaggag cagtgcaagg tagctgtccc gcagattcag ggtctgtgtc
     4561 atcagtacgt agtacggaac aagaccgccg gaaaacaaag tggtaaagta gaagaaaaac
     4621 gaaaactgat ttctccactc aaactcctta cgtgacagta cataagctgt catagtctgc
     4681 agcagcagac caagtaccgt tccgacgatg gtcagaccga tggtcgtgcc gtatgcgcgg
     4741 aagacaacca taggatcttt aaatacggtt ttgtatgcct ccaggctgaa atcctgcggc
     4801 agaaggctga aaccgttctg gataatggcc gattccgaag aaaacgagcc ggatacgatc
     4861 atgatgaaag gaacgaggca gaccagcgcg atgattccgc agaatccgta cgcgatgata
     4921 tcaagtgcaa ccgcatcttt tcctcttttg attttacttg tatgactgga agatcccact
     4981 tttcatccct cctagaataa ggtgtagtct ttatcataaa gaccaaccag tttgtttacg
     5041 attacgacag tgataaagca cagtaccgac tgatacagac caacagctgc ggacattccg
     5101 aagtcgttgc tgctgattaa ggcacggaac gtcagggtat caattacatc ggtgtagttg
     5161 tacagaagac cgttacttcc aaccaggttg tagaacatat caaagtttcc tttgaagatg
     5221 cctccgacgg atagcagaag caggatgatt gtggtcggca tgatcatcgg gattgttact
     5281 ttgaaaattc gctggaacac gtttgctccg tcgatgcggg ctgcttcata aatcgaagtg
     5341 tcaattccca taatcgctgc caggtacata acagaaccgt atccgattcc cttccataca
     5401 ttaaagaaga caaggataaa cggccatgcc tttggtgtcg tgtaaaactt gattggtgtt
     5461 ccaccgaagg tttcaatcag tcggtttaga aaaccgtagt cggaactgaa aatattgaat
     5521 gccataactc ctacgataac ccaggaaatg aaatacggca gaaacatcag cgactgtgtg
     5581 atcttacggt aatgtttgtt gcggatctcg gtcagaagca cggcaacggc aatctgcatc
     5641 gtcgtgttga tcgcaataaa tgccagatta tacagcacgg tatttctggt aacgagtccg
     5701 gcctgtcctg atttaaagaa gaacttgaag ttatcaaatc cgttccacgg gcttccccag
     5761 atacctcctg cgtaggagta ttttttgaat gccattacaa ttcctgtcat cgggtagtag
     5821 gaaaaaatca gggtataaat tactgccggt gcaagcatca gaagaaatac ccggtacttg
     5881 tagagctttt tgcaaaaccc tttccaacca ttctttttca tcttctcatt ccctttcttg
     5941 tttgcgcaac ttttttgttg ctcaactctt tatttttctt tgtgccatta tcatatcatt
     6001 tcggcatatt gtttacaatt cgcagttttt ataagatatt tcttgtcgaa atatactgtt
     6061 ttactatttt ttgtgcaaaa tgcgcaactt tagcgtttta ccttgttgcg cattgatttt
     6121 tccattaccg tttgcgattt tcacgttctc ttttgatatt ccgtattttt tcttgtgtat
     6181 tctgccaaat attctgcaaa ataacttcct caattccgaa aatcccgttg taagaaaaga
     6241 aaaagtgcgc aacgtttcgc attttcgttg cgcactttct ggtttctatt cactttttcc
     6301 ctgtttctgc cttatttcgc agcaatgaca tcctgcatgt catacatgcc agccggtttt
     6361 ccagccagga attttgcagc ctctacagct ccttttccga aaactccttt ggaatatgct
     6421 ctgtgggaca gggtaacaac ctcgtccggg cctgcgaaga tga
//
LOCUS       DAOMUF010000117         6402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3271_length_6402_cov_4.960611, whole genome shotgun sequence.
ACCESSION   DAOMUF010000117 DAOMUF010000000
VERSION     DAOMUF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3271_length_6402_cov_4.960611"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(531..1271)
                     /gene="nagB"
                     /locus_tag="PLN77_07420"
     CDS             complement(531..1271)
                     /gene="nagB"
                     /locus_tag="PLN77_07420"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRM44137.1"
                     /translation="MLLIEAKDYNDMSRKAANIISAQVIMKPHAVLGLATGSTPVGAY
                     KQLVEWYNKGDLDFANVTSVNLDEYKGLSPENDQSYRYFMNTNLFNHVNINKERTFVP
                     NGLEPDSEKACAAYNETIRSVGGIDLQLLGLGNNGHIGFNEPGAAFEKETHCVDLTES
                     TIQANKRFFEKVEDVPTQAYTMGIKNIMQARKVLLIVSGEGKAEILDKVLYGPVTPQV
                     PASILQLHNDLTVVADEAAMSVIKANHR"
     gene            complement(1306..2415)
                     /gene="nagA"
                     /locus_tag="PLN77_07425"
     CDS             complement(1306..2415)
                     /gene="nagA"
                     /locus_tag="PLN77_07425"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRM44138.1"
                     /translation="MIIKNGRVFQEDGTFAEKDLYIEGKRFVSTKEEVIDQEVIDAKG
                     MMVLPGLVDVHSHGAAGHDFSDGDVEGLKEILRYEKAHGITSYCPTSMTLPKDDLLKI
                     FGTIEAIKDEPEAEVIAGVNMEGPFIDPIKKGAQAEENIVAPNAEFFRACNAASGGKI
                     RLVTLAPNMPGSLEFIKEVSDEVMVSIGHTTADYDTALAAMKAGAHHVTHLYNAMPPF
                     AHRNPGVIGAAFDDPECRMELICDGYHIHGAVVRATFSMMGSERMVLISDSMMATGMP
                     NGTYSLGGQAVWMKDGKATLTDGETIAGSATNLYDCMRKAVSFDIPLADAIFAATRNP
                     AQSIGIYDEVGSIAPGKKAHVLLVDDELNLKQVIM"
     gene            2658..3473
                     /locus_tag="PLN77_07430"
     CDS             2658..3473
                     /locus_tag="PLN77_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM44139.1"
                     /translation="MEHFEKSIIPVIESVYPSFTPLERTVADFFIHNTDESDLSARHV
                     SELLYVSEASLSRFAKKCGYTGYREFVYRYQEGLNAALPGTDDHIKQVLNTYQELLNK
                     SYSLADRAQIDRICHILASKRRVYVYGLGSSGLSAQEMKLRFMLAGVDIEAITDIHIM
                     KMNAVLLDESCAAIGITVSGRTPEVLRALGAAKEKGASTILFTSRSSSDYDAFCDEVL
                     LLAVKEHLKNGGAISPQFPILVMIDVLYSHFLTTDSERKEALYEYAVTKLHET"
     gene            3439..3903
                     /locus_tag="PLN77_07435"
     CDS             3439..3903
                     /locus_tag="PLN77_07435"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRM44140.1"
                     /translation="MNTPSPNSMKRDPGRGSAYLSFLKKRCRDFFFFCLLLGVYVAFI
                     TLTGIGCPIWFSTGISCPGCGMSRAVLLLLSGHFQAAFRMHPLVYAVLPAGVWLFFGP
                     EQTAGLRRFKKGIITVLVVLLLAVYAWRIYYHDPLLSIDPSLGRVLQWFYLN"
     gene            3919..4317
                     /locus_tag="PLN77_07440"
     CDS             3919..4317
                     /locus_tag="PLN77_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HRM44141.1"
                     /translation="MNVKDNRSFWVYLLLSIVTCGLYSIYFWYVYVEDLNTIFYGDGE
                     DSPNYIIVLLLSWVTCGIYGVYWRYKQANRMYRESYDRYGVMIEENGSAILLWTLLGY
                     LTGGIGQLVADYFMIQNLNKTALVYNSRAY"
     gene            4336..5688
                     /locus_tag="PLN77_07445"
     CDS             4336..5688
                     /locus_tag="PLN77_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44142.1"
                     /translation="MATSMVKGNPVKLMLSFAFPLLIGNLLQQTYNIIDAAIVGQALG
                     PEALASVGASSSVQFLVLGFCMGSCTGFSVPVAKYFGAGKLDKMRDYIFNGAALAVLI
                     AAILTTLCSLLCAPILHLLSVPSDIFADAYAYQLVIFLGIPFSILYNYLSSILRAVGD
                     SRTPFLFLAFSAILNIFLDLFCIIILHLGCAGAAIATISAQAISGLLCLFFISRKVKL
                     LLPTKENRILRKDAAQELLAMGIPTGLQFSITAIGSMVMQSANNGLGSVYVSAFTAAM
                     KIKQFMMCPFDAISTAASVFCSQNLGAGQAKRIHQGLRQGVLVGVGYGAFAGVIMILF
                     GLPLTKLFIVSSAFEAINASAKYLRCLGFFYWMLGILNVCRMVTQGLGYSGRAVFSGV
                     MEMLARTIVCLGFVGAFGFTAICFADQAAWLAACCYIAPTCLYCVKKSTKMIADRGAK
                     "
     gene            complement(5869..6402)
                     /locus_tag="PLN77_07450"
     CDS             complement(5869..6402)
                     /locus_tag="PLN77_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRM44143.1"
                     /translation="MEKQTGCVHIYCGDGKGKTTCGMGLCTRAAGYGYRVLIYQFMKD
                     NSTSERKVLLLSPNVTFVPGQDKIKFSFLMTPEEKAEQKRYYEEQFQKVTEKAVQEEY
                     DVLFLDELVYTIGSKLFDEQLLLDFLDHKPEKLEVILTGQGPSEALIERADYVSELKK
                     IKHPFDKGLAARDGIER"
BASE COUNT         1318 a         1674 c         1527 g         1883 t
ORIGIN      
        1 atgtgcggct tgccggatgt gtaggcaatc gccgtggtga tataatattt cggtctgatt
       61 ttttccattg ctttcatttc tcctttcaaa taaaaaaact gccttattcc gctctcttgt
      121 agtcggaaga agacaggttt gtttacccgt gataccactt cttttcgtcc ctgcctcacg
      181 gcagaaacct ctgcaggtac atgtgctata cctctccgct gtaacaggcg gaacctgtcg
      241 cagccttacc gtttcagatt gttgttctca cggttcggtg cgcctctcgg aagccatgtt
      301 cgatctgccg ctcctcctcc cctctcagcc tgcaaaaccg gaatggtttt cgggggtgtt
      361 ctctgtaaat tcgcacatgc agatgtactc tcttcgtcac tgtgttttgt ttttatgcac
      421 tggttttatg ctaacacatt tacatcaatt tgtaaaccgc aaaaaagccc gccgtacggg
      481 gattttttca tattctcccc attcggcgga cctttcattc atcgtaacaa ttatctgtgg
      541 tttgctttga tcacagacat tgctgcttca tctgctacaa ctgtcagatc gttgtgaagc
      601 tgcaggatgg aagccggaac ctgcggagta accggtccgt acagaacttt gtcgaggatc
      661 tctgcttttc cttcgccgct gacgatcagc agaacttttc ttgcctgcat gatgttcttg
      721 attcccatgg tgtatgcctg ggtcggaaca tcctctactt tttcaaagaa acgtttattt
      781 gcctgaatgg tgctctcggt caggtcaacg cagtgtgtct ctttctcgaa tgctgcgccc
      841 ggctcgttga atccgatgtg gccgttgttt ccaagtccaa gaagctgcag gtcaatgccg
      901 ccgacgctgc ggatggtttc gttgtatgct gcacatgctt tttcgctgtc cggctccagt
      961 ccgttcggaa cgaaggtgcg ctctttgttg atgttgacat gattgaacag gttggtgttc
     1021 atgaagtaac gatagctctg atcattctcc ggagagagtc ctttgtactc gtccaggttt
     1081 acgctggtga catttgcgaa atcaaggtcg cctttgttgt accactcaac gagctgtttg
     1141 taagcgccga ccggggtaga tccggttgca aggccgagca ctgcgtgtgg tttcatgatc
     1201 acctgcgcgg agatgatatt tgctgctttt ctgctcatat cattgtaatc ttttgcttca
     1261 attaataaca tttgtttttc ctccaaattt cttctatata ataaattaca taataacctg
     1321 tttcaggttc agttcgtcgt ccaccagaag tacatgcgct tttttgcccg gtgcaatgga
     1381 tcctacttca tcgtagatac cgatgctctg tgccggattt cttgttgccg caaaaatggc
     1441 atctgccagc ggaatatcaa acgaaactgc ttttctcatg cagtcgtaca ggttcgtagc
     1501 ggaacccgcg atcgtctcgc cgtcagtcag tgttgctttt ccgtctttca tccagacagc
     1561 ctgtcccccg agagaatacg ttccgttcgg cattcccgtt gccatcatgg aatcgctgat
     1621 gagaaccatg cgctcacttc ccatcatgga gaaggtcgcg cggacaaccg caccgtgaat
     1681 gtgatagccg tcacagatca gctccatgcg gcactccgga tcgtcgaatg cggcgccgat
     1741 gacaccaggg ttacggtgtg cgaacggcgg catggcgttg tacagatggg tgacatggtg
     1801 tgctcctgct ttcatggcag ccagcgcggt gtcataatct gctgtcgtat ggccgatgga
     1861 aaccatgact tcatcggaaa cctctttgat aaattccagt gagccaggca tattcggggc
     1921 cagggttacg agacggattt ttccgccgct cgccgcattg catgcgcgga agaactcagc
     1981 gttcggagcc acgatgttct cctctgcctg tgcgcctttt ttgatcggat cgatgaacgg
     2041 tccttccatg ttgacgccgg cgatcacttc tgcttccggc tcatctttga ttgcttcgat
     2101 ggttccgaag attttcagca gatcgtcttt tggcagtgtc atggatgtcg ggcagtaaga
     2161 ggtgatgccg tgcgctttct cgtagcgcag gatctctttt aaaccttcca cgtctccgtc
     2221 tgaaaaatca tgtccggcag cgccgtggct gtgtacgtca accagtcccg gcagaaccat
     2281 catgcctttc gcatcgatca cttcctgatc gatcacttct tcttttgtgg aaacgaaacg
     2341 ttttccttct atataaagat ctttttccgc aaacgttccg tcttcctgaa atacacgtcc
     2401 gtttttgata atcattgtct tgccttcttt ctttgatctg ttctgattat aatttatgtt
     2461 gattttattt tcaactattt ttatatttta gaaattactt tcacgtcatt aaagttcttt
     2521 ccatttttcc gcccgctttc ccgtgcaatt cacacacaat tgaaagttga ttcaccactc
     2581 tgcctgtgat acaataaagc aagaactgat ctgaattttt ttcagatatt tacagatacc
     2641 aggaggactt acgttccatg gaacactttg aaaaatcgat tattcctgtc attgagtctg
     2701 tctatccctc gttcactccc ctggaacgga cggtagcaga tttttttatt cataataccg
     2761 acgagtcgga tctgtccgcg cgccacgttt cggaactgct ctatgtttcg gaggcttcgc
     2821 tctcccgctt tgcgaaaaaa tgcggctaca cgggctaccg cgaatttgtc taccgttacc
     2881 aggaaggtct aaacgccgca ctgcctggca cggacgatca catcaaacag gtgctgaata
     2941 cgtatcagga gctgctgaat aaaagctact cgctcgccga ccgcgcgcag attgaccgca
     3001 tctgccatat tctcgcttcg aagcgtcggg tgtatgttta cggtctggga agctcgggac
     3061 tttctgcgca ggagatgaag ctgcggttta tgcttgcggg cgtggatatt gaagcaatta
     3121 ccgatattca catcatgaaa atgaacgcgg ttctgcttga tgagagctgt gcggcgatcg
     3181 gcatcaccgt cagcggacgg acgccggagg tgctgcgggc actcggcgct gcaaaggaaa
     3241 agggggcctc gacgattctc tttacctccc gcagttcgag cgattacgat gccttctgcg
     3301 atgaggtgct gctgctggca gtcaaggagc atctgaagaa cggcggcgcg atctctccgc
     3361 agttcccgat tctcgttatg atcgacgttc tgtactcgca ttttctcacg accgacagcg
     3421 agcgcaagga ggctttatat gaatacgccg tcaccaaact ccatgaaacg tgatcccggc
     3481 cgcggcagcg cgtatctttc ttttctgaag aaacgctgcc gcgatttctt tttcttctgc
     3541 ctgctcctcg gcgtttacgt cgccttcatc accctcacag gcatcggctg tccgatctgg
     3601 ttttccaccg gcatttcctg cccgggctgc gggatgagcc gtgcggtact gcttctgctc
     3661 tcgggtcatt ttcaggcggc gtttcggatg catccgcttg tctatgccgt gctgcccgca
     3721 ggtgtctggc tcttcttcgg gccggaacag acggcgggac ttcggcgttt taaaaaaggg
     3781 atcattacgg tgcttgtcgt gctgcttctc gccgtttatg cctggcgcat ttattaccac
     3841 gacccgctcc tttctattga cccctctctc gggcgcgtgc tacaatggtt ctatcttaat
     3901 taaagggggt atttacaaat gaatgtaaaa gacaaccgca gtttctgggt ctatcttctg
     3961 ctttcgattg tcacctgcgg actctactcg atctacttct ggtatgttta tgtggaagac
     4021 ctgaacacca tcttttacgg tgacggcgaa gacagcccga actacattat tgtgctgctt
     4081 ctgtcctggg ttacctgtgg aatttacggc gtttactggc gttacaaaca ggcgaaccgc
     4141 atgtaccgcg aatcctacga ccgctacggt gtgatgattg aggaaaacgg ttccgccatt
     4201 cttctgtgga cgctgcttgg ctacctgacc ggcggcatcg gccagctcgt tgccgactat
     4261 ttcatgatcc agaacctgaa taagacggca ctcgtttata attcacgtgc atattaagca
     4321 ggggaggcgt tttttatggc aacatctatg gtaaagggaa atccggtaaa gctgatgctc
     4381 tcgtttgcct ttccgcttct catcggaaat ctgctgcagc agacatataa catcatcgat
     4441 gcggcgattg tcggccaggc acttggcccg gaggcgctcg ccagcgtcgg tgcaagcagc
     4501 agtgtacagt ttctggtgct cggcttctgt atgggaagct gcacgggatt cagtgttccg
     4561 gttgccaagt atttcggcgc cggaaagctt gataaaatgc gcgattatat ttttaatggt
     4621 gctgcacttg ctgtcctgat tgcggccatt ctgaccacac tctgcagttt actctgtgca
     4681 ccgattctgc acctgctgtc ggttccgtcc gatatcttcg cggatgccta cgcatatcag
     4741 cttgtgattt tcctcgggat tccgttttcg attctgtaca attatctttc cagcatcctg
     4801 cgtgccgtcg gggacagccg cacgccgttt ctgtttctgg cattttcggc aatcctgaat
     4861 attttccttg atctgttctg tattatcatt ctgcatctgg gatgcgcagg cgcggcgatt
     4921 gcaacgattt ccgcccaggc gatcagcgga ctcttatgtc tgttttttat ttcccgtaag
     4981 gtaaagctcc tgcttccgac aaaggaaaac cgcattctgc gaaaggatgc cgcgcaggaa
     5041 ctcctcgcaa tgggcattcc gacgggactg cagttttcga tcacggcgat cggcagtatg
     5101 gtaatgcagt cagcgaataa tggacttggc agtgtttatg tttcggcgtt cacagctgct
     5161 atgaaaatca aacagtttat gatgtgtccg tttgatgcga tcagtaccgc ggcttccgtc
     5221 ttctgcagtc agaatctcgg agcaggccag gcaaagcgaa tccatcaggg actccgccag
     5281 ggtgtgcttg tcggtgtcgg ctatggtgcg tttgcgggtg tcatcatgat tctgttcgga
     5341 ctgccgctga caaagctctt tattgtcagc tctgcttttg aagcaatcaa cgcttctgca
     5401 aaatatctgc gctgcctcgg atttttctac tggatgcttg gtattttgaa tgtctgccgt
     5461 atggtaacgc agggacttgg ctactcggga agagccgttt tctccggcgt aatggagatg
     5521 ctggcacgaa cgattgtctg cctgggattt gtcggtgcgt ttggcttcac cgcaatctgc
     5581 tttgcggacc aggctgcatg gctcgccgcc tgctgctata tcgcaccgac atgtctgtac
     5641 tgcgtgaaga aatccacgaa aatgattgcg gatcgcggtg cgaaataata acgttatttt
     5701 ctgtcttaca gcggcttcca gatacttctc aggttatcgc ggcaatcacc agaaacttgt
     5761 tccatcacag attttggttc attgcccgca aaaacaagaa aaaagatact gttctgcatg
     5821 gaatcatctt ccccgcaaaa cagtatcttt tttcttgttt tttcagcgtt accgctcgat
     5881 tccatctctt gccgcaagac ccttgtcaaa cggatgcttg atttttttca gctccgaaac
     5941 atagtccgcc cgctcaatca gcgcctccga cggtccctgc ccggtcagaa tcacttccag
     6001 cttctccggc ttgtgatcca gaaaatccag aaggagctgc tcatcaaaca gtttgcttcc
     6061 aatcgtataa accagctcgt ccagaaacag tacatcgtat tcttcctgaa ctgctttttc
     6121 tgtcactttc tgaaactgct cttcataata ccgtttctgc tctgccttct cctccggtgt
     6181 catcagaaac gaaaacttta tcttgtcctg ccccggcaca aacgtcacat tcggcgacag
     6241 taagagcacc ttccgctcgc tcgtgctgtt atctttcata aactgataaa tcaaaacacg
     6301 gtatccatac cctgccgcac gcgtgcagag tcccatacca caagtcgtct ttccttttcc
     6361 atccccacag tagatgtgga cacagcctgt ctgtttttcc at
//
LOCUS       DAOMUF010000118         6390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3287_length_6390_cov_4.392739, whole genome shotgun sequence.
ACCESSION   DAOMUF010000118 DAOMUF010000000
VERSION     DAOMUF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3287_length_6390_cov_4.392739"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLN77_07455"
     CDS             complement(<1..290)
                     /locus_tag="PLN77_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRM44144.1"
                     /translation="MKRRVVITGLGAVTPIGNTVEEFWNGVRKGACGIGTITKFDTAE
                     YKVHLAGEVKGFVAKERMDFKAARRMGTFSQYAVAAAKEAFADAGISMENEDP"
     gene            complement(307..1047)
                     /gene="fabG"
                     /locus_tag="PLN77_07460"
     CDS             complement(307..1047)
                     /gene="fabG"
                     /locus_tag="PLN77_07460"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRM44145.1"
                     /translation="MLSGKTAVVTGASRGIGRAIAEKLAAEGAFVVINYNGSKEAAET
                     VLAGIRAAGGDGCVMQCNVSDFAACEAWMKEILALYGKIDILVNNAGITKDGLLMGMS
                     EEAFDRVIETNLKGTFNCIRFAARPMIRQKSGCIINMASVVGVAGNAGQANYAASKAG
                     VIGLTKTAARELASRGIRVNAIAPGFIETDMTEVLSDKVKEASVSQIPLGHFGKPEDV
                     ANLAAFLASEAAAYITGQVIHCDGGMVI"
     gene            complement(1076..1996)
                     /gene="fabD"
                     /locus_tag="PLN77_07465"
     CDS             complement(1076..1996)
                     /gene="fabD"
                     /locus_tag="PLN77_07465"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRM44146.1"
                     /translation="MSKTAFMFPGQGSQCVGMGADFYEACPKARAVYDMASELIGIDM
                     KKLCFEENEHLDQTEFTQIALLTTGMAMEQSIRACGLTPDVTAGLSLGEYNAIVSAGG
                     MEMADAMKVVRRRGILMEEAVPAGEGAMAAVLGMEDAKIEEILSGISGAYIANYNCPG
                     QIVITGYEAAVAEASEKLKEAGAKRVLPLNVSGPFHSPMMEAASQGLTEALEGVGFME
                     LKIPYVTNVTGQYVRDTALTRGLLIQQVASGVRWQQSIEAMIADGVDTFVEIGPGRTL
                     TGFLRKINRDVKGYNIRTYEEMHQVCETLL"
     gene            complement(1993..2931)
                     /gene="fabK"
                     /locus_tag="PLN77_07470"
     CDS             complement(1993..2931)
                     /gene="fabK"
                     /locus_tag="PLN77_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HRM44147.1"
                     /translation="MITEVTKLLGIQYPVIQGGMAWVAEHHLAAAVSEAGGLGLIGAS
                     AAPAEWVRNEIREAKKLTDKPFGVNIMLMSPYADEVAKVVAEEGVKVVTTGAGNPEKY
                     MELWKNAGIRVIPVVASVALARRMERAGADAVVAEGCESGGHIGETTTMALVPQVADA
                     VKIPVIAAGGIADGRGMAAAFMLGAEAVQMGTRFVASKEAVVHENYKQLVIKAKDIDT
                     RVTGRTTGHPVRAIRNEMTRHYLELEKEGAPFEELEKLTLGGLRRAVVDGDVKTGSVM
                     SGQIAGMVKDEKSCKEIIEQMMNEMEELLKGAPARL"
     gene            complement(2962..3189)
                     /gene="acpP"
                     /locus_tag="PLN77_07475"
     CDS             complement(2962..3189)
                     /gene="acpP"
                     /locus_tag="PLN77_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRM44148.1"
                     /translation="MLEKVKETVAEALGADIDTITAETSFKEDLGADSLDLFEMVMSL
                     EDTCGVEIPTEDLETMVTVGDVAEYLEQHKN"
     gene            complement(3240..4169)
                     /locus_tag="PLN77_07480"
     CDS             complement(3240..4169)
                     /locus_tag="PLN77_07480"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRM44149.1"
                     /translation="MKNGRIAGTGSYVPETIWKNEDLEKMVETSDAWIRERTGIGARH
                     IAGEETVAQMAVKAARRALENAGICSTELDLILVGTSSSESIFPNTACLVQDEIGAFR
                     AACLDVSAACTGFLAVYELGQLYIRSGKAKNVLLIGADALSRLVDWSDRGTCILFGDG
                     AGAVVLTAEEQETKACEKIHSDGEKGVSLTCEKGTYLQMDGRAVFQFALSRVPEVIRE
                     VLAEADMAVEEIDAFILHQANSRIIDGVAKRLKAPKEKFPRNIEAYGNTCAASIPILL
                     DEWNRSGRAQKGQRIVLSGFGAGLIWGAAYLEW"
     gene            4414..5835
                     /locus_tag="PLN77_07485"
     CDS             4414..5835
                     /locus_tag="PLN77_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRM44150.1"
                     /translation="MQVPMSSYLVEIKPQIQELIRLLEREFDYVNVLCTDVKGTTYRV
                     SMHQTTVGDYHFCERGFVVRAWQDGSYTEYSFNNLTDAADLAEEITSALKSEFQALKA
                     LGIAQMESPLVQEEAITKTMQNEIEIDPETVSAEEILSHLRKLCDAGAAHEGILEFQS
                     TVSFARVNKLFLSSKKDLMQSYAFSEGSLSAIGTENGKQNMSYRSCSGLKGVEILNEM
                     DAIVEEIIAVLYDKLHSDPVTPGMYDVITAPDVTGVIAHEAFGHGVEMDMFVKDRALA
                     KEYIGKPVASQLSSMHDGAVGAQQVSSYLFDDEGTLGTDTTVIDHGTLVTGISDLVSA
                     LRLGTTPTGNGKRESFERKVYSRMTNTYFTAGTDKLDDMIASIEHGYLLESVQSGMED
                     PKHWGIQCMVGLGREIKDGKLTGKVVSPVTLTGYVPDLLKSISMVSDKEELFGSGMCG
                     KGHKEWVKVSDGGPYLKCKVRLG"
     gene            5835..>6390
                     /locus_tag="PLN77_07490"
     CDS             5835..>6390
                     /locus_tag="PLN77_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44151.1"
                     /translation="MNELYDVLKTSKASDFLIREEQTESREAFFIGQKLDMSRAKKVT
                     HTFLTVFVDSEDGKFRGSAEKEIHQGISREEMQQEIDQALFAAQFVQNPWYPIAEPSD
                     APANDGMPDASRDLTSELVKLVQAMQEIKSEEDSRVNSYEIFVNQKQTHIRNSRGVDV
                     TFSGFDCEIEVVTNSRKDDHEIEIY"
BASE COUNT         1386 a         1945 c         1449 g         1610 t
ORIGIN      
        1 ggatcttcat tctccatgga aatgccggca tctgcaaacg cttcttttgc tgccgcaacg
       61 gcatactggg agaaagttcc cattcgtctt gccgccttga aatccatgcg ttcttttgcc
      121 acgaagcctt tgacctcgcc tgccagatgt actttatatt ctgctgtatc aaatttcgta
      181 atcgttccga ttccgcaggc tcccttgcgg acgccgttcc agaattcctc cacggtattt
      241 ccaatcgggg ttaccgcacc cagccctgtg atgactactc ttctcttcat tccaactctc
      301 cttttcttag atcaccatgc cgccgtcgca gtgaatcacc tgtccggtga tgtaagctgc
      361 cgcttcggat gccagaaatg cagccaggtt tgccacatcc tccggctttc cgaaatggcc
      421 gagcggaatc tgggatacgg atgcttcttt cactttatcc gaaagaacct ccgtcatatc
      481 ggtctcaata aagcccggcg caatcgcatt gacgcggatg ccgcgggacg ccagttcccg
      541 tgccgctgtt ttcgtcaggc cgatcacacc tgctttcgat gccgcataat ttgcctgtcc
      601 ggcgtttcct gccacgccga ccacagaagc catgttgatg atgcagccgc tcttctgacg
      661 gatcatcggc cgtgccgcaa agcggatgca gttgaacgtt cccttgaggt ttgtctcaat
      721 gacacggtcg aaggcttcct ccgacatgcc catcagaagg ccgtcctttg tgatacccgc
      781 gttgttgacc agaatatcga ttttcccata caaagcaaga atttccttca tccacgcttc
      841 acacgccgca aaatccgata cattgcactg catcacacag ccgtctcctc ctgccgcacg
      901 gattcccgca agcacggttt cggccgcctc tttcgaacca ttgtaattga tgacaacaaa
      961 ggctccctcc gccgccagtt tttctgcaat cgcacgcccg attccgcggg atgcgcccgt
     1021 tactactgct gtttttccac ttaacatctc attctcctgc tgcataatcc actatctata
     1081 ataaagtttc gcacacctga tgcatttcct cataggtgcg gatattgtaa ccttttacat
     1141 cccggttgat ttttcttaaa aagccggtca gtgttcttcc cggtccgatc tccacgaagg
     1201 tatcaacgcc gtccgcaatc attgcctcga tgctctgctg ccaccgcaca ccggatgcca
     1261 cctgctggat cagaaggccg cgcgtcagtg cagtatcacg cacatactgg ccggttacat
     1321 tcgtcacata cggaatcttc aactccataa agccgacacc ctcgagcgct tcggtcagcc
     1381 cctgggacgc agcttccatc atcggggaat ggaacgggcc gcttacgttc agcggaagta
     1441 cccgctttgc gcctgcttct tttaactttt ccgaagcctc tgccactgcc gcctcatatc
     1501 cggtgatgac aatctgaccc gggcagttgt aattggcaat gtaggcgccg gaaatcccgc
     1561 tcaaaatctc ttcgattttt gcatcttcca tgccgagcac cgccgccatc gcgccctcgc
     1621 ctgccggaac ggcctcctcc atcaggatgc ctcttctgcg gacgaccttc atcgcatccg
     1681 ccatttccat accgccggcg gacacaatcg cgttgtactc gccaaggctg aggcccgctg
     1741 tcacatccgg cgtaaggccg cacgcccgga tggactgctc catcgccatt ccggttgtca
     1801 gcagtgcaat ctgggtaaac tctgtctgat ccagatgttc attttcctca aaacagagtt
     1861 ttttcatatc aatcccgatc agctcggatg ccatatcgta gacggctctc gctttcggac
     1921 atgcttcata aaaatccgcg cccatgccga cacactggga accctgtccc ggaaacataa
     1981 atgctgtttt actcataacc gcgccggtgc tcctttcaga agttcttcca tctcattcat
     2041 catctgctct atgatttctt tgcagctttt ctcgtctttt accatacctg caatctgacc
     2101 ggacatcacg cttccggttt tgacatcgcc gtccacaacg gctcttcgaa gaccgccgag
     2161 cgtcagtttt tccagctctt cgaacggagc tccctctttt tccagttcca gataatggcg
     2221 tgtcatctca tttcggatcg cccgcaccgg atgaccggtt gtgcggcctg tcacgcgggt
     2281 atcaatatct ttcgccttga tcaccagctg cttgtaattt tcatgcacaa cggcctcttt
     2341 cgaagcgaca aaacgcgttc ccatctgaac cgcctcggca cccagcataa aggctgctgc
     2401 catgccgcgt ccgtccgcaa ttccaccggc cgcaatgacc ggaattttta cggcatctgc
     2461 cacctgcgga acgagtgcca tcgttgtcgt ctcgccgata tggccgccgg actcacatcc
     2521 ttctgccaca acggcatctg cgcctgcgcg ctccattcgt cttgcaagag ccacggaagc
     2581 gaccaccggg atgacgcgga taccagcgtt tttccacagt tccatatatt tctccggatt
     2641 tcctgcgccc gtcgtaacga cttttacgcc ttcctcggca accacttttg ccacctcatc
     2701 cgcatacggg ctcatcagca tgatgttgac gccgaacggt ttgtccgtca gtttttttgc
     2761 ttcgcggatt tcattccgaa cccactctgc cggtgccgcc gatgctccga tgaggccaag
     2821 gccgccggcc tcggaaactg ccgctgccag atgatgctcc gccacccagg ccatgccgcc
     2881 ctgaatgact ggatactgaa tcccgagtaa ttttgtcact tctgtgatca tgaatcgctt
     2941 cctcctgctt cccgatcttt cttagttttt gtgctgctcc agatactctg caacatcacc
     3001 gacagttacc atcgtttcca gatcctcggt cgggatttcc acgccgcagg tgtcctcgag
     3061 ggacatgacc atttcaaaca gatccaggga atctgctccg agatcctctt tgaaggaagt
     3121 ctccgctgtg atggtatcga tgtctgctcc cagtgcttct gctacggttt cttttacttt
     3181 ttctaacatg tttttgtcct cacttttttc ttattcttct tattttatat gggcgtttct
     3241 taccattcca ggtacgccgc gccccagatc agtcctgctc cgaaaccgga gagaacgatt
     3301 ctctgtcctt tttgggctct gccggaccgg ttccactcat ccagcagaat cggaatgctc
     3361 gccgcacacg tattgccgta tgcttcgata ttccgcggaa atttttcttt cggcgccttc
     3421 agtcgttttg ccacgccgtc gatgatgcgg ctgtttgcct gatgaaggat aaacgcatca
     3481 atctcttcga ccgccatgtc ggcttccgcg agcacctcgc ggatgacttc cggcacgcgt
     3541 gataaagcaa actggaacac ggcacggccg tccatctgca gatacgttcc cttttcgcag
     3601 gtcagcgaca cgcctttttc tccatcggaa tggatctttt cgcacgcttt tgtctcctgc
     3661 tcctccgccg tcagcacaac ggctcccgcg ccgtccccga acagaatgca ggttccgcgg
     3721 tcactccagt ccaccaggcg gctcagggca tccgcgccga tcagcagcac gttttttgcc
     3781 ttgccgctcc ggatatacag ctggcccagc tcatacaccg caagaaatcc ggtgcaggcg
     3841 gcgctgacat cgagacaggc ggcacggaag gcaccgattt catcctgcac aagacaggcg
     3901 gtattcggaa agatgctctc cgacgaggag gttccgacga gaatcagatc caattctgtc
     3961 gaacagatcc cggcattttc cagcgctctt ctcgccgcct ttactgccat ttgggcaacc
     4021 gtctcctccc cggctatgtg gcgcgcaccg attcctgtcc gctcccggat ccaggcatcg
     4081 ctcgtctcca ccatcttttc cagatcttca tttttccata tcgtctccgg tacatacgaa
     4141 ccggttcccg caattctgcc gtttttcacc ttactgtcct cttgcttatt tcattctcaa
     4201 acatttttct tttcaaatgt tttgatattc aaagtatatc atcaaaattc attttgtcaa
     4261 ccttttttta ttgctttttc catttcccac ttctataatg aaactatgtt cgggattttt
     4321 tcgaaactat tttttccgga ttctattatt ttgagccccg gcgcaggttc tctatcgaac
     4381 actccggcag gctccagaaa ggacaccaaa cttatgcagg ttccaatgtc atcctatctt
     4441 gtggaaatca aaccacagat tcaggaactg atccgtctgc tcgagcgcga atttgattac
     4501 gtcaacgtgc tctgcaccga tgtcaaaggt acgacctacc gcgtcagcat gcatcagacc
     4561 accgtcggcg attatcactt ctgtgaacgc ggcttcgttg tccgtgcatg gcaggacggt
     4621 tcctacaccg aatattcctt caacaacctc accgatgccg cagatcttgc ggaggaaatt
     4681 acttccgcgc tgaaatccga atttcaggca ttaaaggcac tcggcattgc gcagatggag
     4741 tctcctctcg tgcaggaaga agccatcacc aaaacgatgc agaatgaaat cgagattgac
     4801 ccggagaccg tctccgcaga ggagattctc tcccaccttc ggaagctctg cgacgccggc
     4861 gctgcccatg agggaattct ggaattccag agtaccgtca gctttgcccg tgtcaacaag
     4921 ctgttcctct cctccaaaaa ggatctgatg cagtcctacg ctttctcgga gggctccctt
     4981 tccgcaatcg gcacagaaaa cggaaaacag aatatgtcgt accgttcctg ctccggcctg
     5041 aaaggcgtgg aaatcttaaa tgagatggat gcgattgttg aggaaatcat cgccgttctg
     5101 tacgacaagc tccactccga tccggtcacc cctggcatgt acgatgtcat caccgcaccg
     5161 gatgtcaccg gcgtgattgc acacgaggca ttcggccacg gtgtcgagat ggatatgttt
     5221 gtcaaagacc gcgcactcgc caaagagtat atcggcaagc ctgttgcctc ccagctctcc
     5281 tccatgcacg acggagcagt cggcgcacag caggtcagca gttatctttt tgatgatgag
     5341 ggaacgctcg gcaccgacac cacggtcatc gaccacggta cactcgttac cggcatcagc
     5401 gatctcgtaa gcgcactccg ccttggcacc acgccgaccg gaaacggaaa acgcgagagc
     5461 ttcgagcgca aagtctactc ccgtatgacc aacacctact tcaccgcagg cacagacaaa
     5521 ctcgatgata tgatcgcttc gattgagcac ggctatcttc tcgaaagcgt tcagtccggt
     5581 atggaagacc cgaaacactg gggcatccag tgcatggtcg gactcggccg cgagatcaaa
     5641 gacggtaaac tgaccggaaa agtcgtatct cctgtcaccc tgaccggcta cgttccggat
     5701 ctcttaaaga gcatctccat ggtttccgac aaagaagagc tcttcggctc cggcatgtgc
     5761 ggaaaaggtc acaaagaatg ggtcaaagtc agcgatggcg gcccgtatct gaaatgtaaa
     5821 gtgaggttag gctgatgaac gaattatacg atgtattaaa aacaagcaaa gccagcgact
     5881 ttctgatccg cgaagagcag accgaatccc gcgaggcatt tttcatcggt cagaagctcg
     5941 acatgagccg cgcgaaaaaa gtaactcata cgtttctgac cgtctttgtc gacagtgagg
     6001 atggaaaatt ccgcggaagc gcagaaaaag agatccacca gggcatttcc cgcgaagaaa
     6061 tgcagcagga aattgaccag gcgctgttcg ctgcacagtt cgtgcagaac ccatggtacc
     6121 cgattgcaga gccatcggat gcaccggcaa atgatggaat gcctgatgcc tcccgcgatc
     6181 ttacctctga actcgtaaaa ctggtgcagg ccatgcagga aatcaaatca gaagaggatt
     6241 cccgcgtaaa ctcctatgag atttttgtca atcagaagca gacacacatc cgcaattccc
     6301 gcggtgtcga tgtcacattc tccggctttg actgtgagat cgaggttgtc acaaactccc
     6361 gcaaggatga tcatgagatt gagatctaca
//
LOCUS       DAOMUF010000119         6228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3388_length_6228_cov_3.307954, whole genome shotgun sequence.
ACCESSION   DAOMUF010000119 DAOMUF010000000
VERSION     DAOMUF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3388_length_6228_cov_3.307954"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..3146
                     /locus_tag="PLN77_07495"
     CDS             108..3146
                     /locus_tag="PLN77_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRM44152.1"
                     /translation="MGVFQYEKVKDPTYYGENRVAAHSDHRYYGSVEEMEQHVENFRH
                     SLNGLWKFHYAKNYESTIPGFETPEYDCTKWDDIRVPAHIQMEGYDAPQYANVQYPWE
                     GREEIQPGEIPERFNPTASYVKYFEVPEQMKGKRLFVSFQGAESGIAVWLNGTFLGYS
                     EDTFTPSEFELTPYLKEGENKLAAQVFKWTSGSWCEDQDFFRFSGIYRDVYLYTIPEV
                     HVSDLKVQTLLDDTFTKADLVIDTKMIGTGKVKITLLKDGTALQSTEGVLDGETQFVL
                     KVDHPELWSAETPVLYDLLLEVTAEDGTVQELIPQRVGFRRFEMKDHIMMLNGKRVVF
                     KGVNRHEFSSVSGRVVSREELIKDLTTMKQNNINAIRTCHYPDAVGIYDLCDEYGLYM
                     IAECNMESHGTRDTDAVRSGDFSGVVPCDRPEWMDCMLDRVNSMYQRDKNHPAILIWS
                     CGNESFGGKVIFEMSQLYRKMDPTRLVHYEGVNDDRRYNDTSDMESRMYTTVNEIREF
                     LSKDRRKPYVCCEYAHAMGNSCGAMKKYMDLAEEEPLFQGGFIWDYIDQSIYKKDRYG
                     KEFQAYGGDFDDHPCDYNFSGNGIVYGGERDASPKMQEVKFCYQNIAVDVQKDKAVVK
                     NKNLFVNTDAFDCVVILEKEGKKLKEVPMEISVEPLSEKTVELPIAVQTLPGEYAVTV
                     SFRLKEDTIWGKRGHEVAFGQGVYEVEAPAKAEKPAKFEVIRSNHDFGVRGENFDVMF
                     SDLNGGLVSYRYGGVEMIKMIPKPNFWRAPIDNDCGSLMPMRYSQWKIASMYLSHKYP
                     NGSHYPGLYAPEIEVHEDCAEVSYRYVMPTTPASECRLTYRVFGDGSIQTTLTYDPVK
                     ELGDMPEFGMMFKLDADYDNVTWYGMGPEETYVDRCAGAKLGVYKNKVEDNMAKYLVP
                     QECGNKVGVRWATVTNRKGRGMMFSGDKMEFSALPYTPHELENAMHPYELPQVHYTVV
                     RVAKQQMGVGGDDSWGAQTHPEYLIPIDKKLEFTFTFKGI"
     gene            3213..5240
                     /locus_tag="PLN77_07500"
     CDS             3213..5240
                     /locus_tag="PLN77_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRM44153.1"
                     /translation="MGKEVNFGKLLHGGDYNPEQWLDYPEILEQDLAYFKKARINEVS
                     LGMFSWAFLEPEEGVFRLEWLKEVIDRLYENEIVVMLSTPTAARPRWMAEKYPEVLRV
                     NAARERNLYGERHNHCYTSPVYREKTRIINTKLAEMFKDHPGVIAWHISNEYGGECHC
                     PLCQEAFRGWVKKKYGTLERLNRVWNTGFWSHTYQSFDQVESPSPKGDFSLHGLNLDW
                     KRFVTDQTADFVKWEIKALRDAGAEQPSTINMMYNFTGLNYYKFADVIDFVSWDNYPT
                     WHKEAETVTAMDTGMQHDIMRSIQKKPFYLMESCPSATNWQSVSKLKKPGMLQAASLQ
                     AVAHGSDSVLYFQMRQSRGASEKFHGAVIDHYGGSDTRVFREVTKVGAALEKLQEVNG
                     SQNPAEAAVIYDVENRWAVEDAAGPRNAGVFYKETVEKPYQAFRKLGINVDVIDETCS
                     LDGYRVVAAPMVYLLREGWTEKVRNFVKNGGIFLLTYWSGIVDETDLCYLGGTPHDLM
                     DVMGFRSMEIDGLYDGEWNEGIPEPENPLHLELAYRCSHLCELVQPSTAEVVMTYGKD
                     FYDGMPAMTRNVYGKGKAYAVCADFEQGLYDEVCRKLAEEAGVERIVEEVPEGVEVTM
                     RKQKDGTRYVFVQNFNREAVAVKLPIERYPVWQGTYDGTIGSWKTIVLKGR"
     gene            complement(5303..5680)
                     /locus_tag="PLN77_07505"
     CDS             complement(5303..5680)
                     /locus_tag="PLN77_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM44154.1"
                     /translation="MSSAEKTPVSLADLPPCPVETTLTLISDKWKVLILRDLLLHGTM
                     RFGELKKSIGHVSQKVLTSQLRQMEQSGLVNRKVYAEVPPRVEYSLTELGFSLHPIMD
                     AMWDWGEAYKKKLAQEEDAAANV"
     gene            5871..>6228
                     /locus_tag="PLN77_07510"
     CDS             5871..>6228
                     /locus_tag="PLN77_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRM44155.1"
                     /translation="MSKAVEFLNENPIQYLATVGRDGKAKCRPFMFAGEVDGKLWFCT
                     NNTKDVYKDMQENPEIEISVSSPSYAWIRLHGTAVFENNMAVKEVCMQNPIVKGQYQT
                     ADNPIFEVFYLDNAHGV"
BASE COUNT         1750 a         1317 c         1787 g         1374 t
ORIGIN      
        1 gtaaaataag aaaaagaatc gaacagaaaa ggtggatatt tggatgaata tccacctttt
       61 tttcaaaaga tcaaaaagaa caaaattcca gaaggaggaa aataaaaatg ggtgtatttc
      121 agtatgaaaa agtgaaggac ccgacgtact atggagaaaa ccgggtggcg gcgcattccg
      181 atcacagata ttatggatcg gtggaagaaa tggagcagca tgtggaaaac ttccgtcaca
      241 gtctgaacgg actgtggaaa ttccactacg caaagaacta cgagagcacg attccgggat
      301 ttgagacacc ggagtatgac tgcacaaaat gggacgatat ccgcgtgccg gcacatatcc
      361 agatggaggg atatgacgca ccgcagtatg ccaatgtgca gtacccatgg gaaggaagag
      421 aagagatcca gccgggcgag attccggagc ggttcaatcc gacggcttcc tatgtgaaat
      481 attttgaagt gccggagcag atgaaaggaa aacggctttt tgtttccttc cagggagcag
      541 agagcggtat tgccgtatgg ctgaacggaa cgttccttgg ctacagcgag gatacgttta
      601 ctccgtccga gtttgaactg accccgtatc tgaaagaggg cgaaaataaa ctggctgcgc
      661 aggttttcaa atggacgagc ggaagctggt gcgaggatca ggatttcttc cgtttctccg
      721 gtatttaccg tgatgtgtat ctgtacacga ttccggaggt tcacgtttcg gatctgaaag
      781 tacagacgct gctggatgat acgtttacga aggcagatct ggtcattgat acgaaaatga
      841 tcggaacagg aaaggtaaaa attaccctgt taaaagacgg aacggcactg cagagcaccg
      901 aaggtgtgct ggatggggaa acacagtttg ttctgaaagt cgatcacccg gaactgtggt
      961 ccgcagagac accggtgctg tatgatcttc tgctggaagt caccgcagag gatggaacgg
     1021 tgcaggaact gattccgcag agagtcggat tccgccgctt tgagatgaaa gaccacatca
     1081 tgatgctgaa tggaaagcgg gttgtcttta aaggcgtcaa ccgccacgag ttcagctccg
     1141 tatccggccg tgtcgtttcg agagaagagc tgatcaaaga cctgacgacg atgaaacaga
     1201 acaacatcaa tgcaatccgt acctgccatt acccggatgc ggtcggcatt tatgatctgt
     1261 gcgatgaata cggtctttac atgatcgcgg agtgcaacat ggagtcccat ggaacgagag
     1321 atacggatgc ggtacgaagc ggagatttct ccggtgttgt accatgcgac cgcccggagt
     1381 ggatggactg tatgctcgac cgtgtcaatt cgatgtatca gagagacaaa aaccatccgg
     1441 cgattctgat ctggtcctgc ggaaatgaat cgtttggcgg aaaagttatt tttgaaatgt
     1501 cccagttata ccggaaaatg gacccgaccc gtctcgttca ttatgaagga gttaatgacg
     1561 accgcagata caacgacacc agcgatatgg aaagccggat gtacaccacc gtcaatgaaa
     1621 tccgtgaatt cttgtcgaaa gacagaagaa aaccgtatgt ctgctgtgag tatgcacatg
     1681 cgatgggaaa ttcctgcggc gccatgaaga aatatatgga tcttgccgag gaagaaccgc
     1741 tgttccaggg cggctttatc tgggattata tcgaccagtc aatctacaaa aaagaccgct
     1801 acggaaaaga gttccaggcg tacggcggag actttgatga tcatccatgc gattacaatt
     1861 tcagcggaaa tggtattgta tacggcggcg agcgcgatgc ttccccgaag atgcaggaag
     1921 taaaattctg ctatcagaac attgctgtgg atgtacagaa agacaaagca gttgtaaaaa
     1981 ataagaacct gtttgtgaat accgatgcgt ttgactgcgt ggtaattctc gaaaaagaag
     2041 gaaagaaact gaaagaagtg ccgatggaaa tttccgtgga gccgctttcc gaaaaaacag
     2101 tggagcttcc gattgccgta cagacccttc cgggcgagta tgcggtgacc gtttccttcc
     2161 gcctgaaaga agataccata tggggcaagc gcggccatga agttgcattc ggacagggtg
     2221 tttatgaagt ggaagcaccg gcgaaagcag aaaaaccggc gaaatttgaa gtgatccgtt
     2281 ccaaccatga ttttggtgtg cgcggcgaaa attttgatgt gatgttctcc gatctgaacg
     2341 gcggactcgt atcgtaccgt tacggcggcg ttgagatgat caaaatgatt ccgaaaccga
     2401 acttctggcg tgcgccgatc gacaatgact gcggaagcct gatgccgatg cgttacagcc
     2461 agtggaagat tgcttcaatg tatctgtccc acaaatatcc gaacggcagc cattatccgg
     2521 gactgtatgc gccggaaatc gaagtacacg aggactgcgc agaagtcagc taccgctatg
     2581 taatgccgac cacaccggcg agcgagtgcc gtctgaccta ccgtgtcttc ggcgatggaa
     2641 gcattcagac gacgctgacg tatgatccgg tgaaagagct cggcgatatg ccggaattcg
     2701 gcatgatgtt caaactggat gccgattatg acaacgttac ctggtacggc atgggaccgg
     2761 aggagacgta tgtggaccgc tgtgccggag caaagctcgg tgtttataag aataaagtcg
     2821 aagataatat ggcgaaatat ctcgttccgc aggagtgcgg caacaaggtt ggtgtacgct
     2881 gggcaaccgt gacgaaccgc aagggccgtg gtatgatgtt cagcggcgac aagatggaat
     2941 tttccgcact gccgtacaca ccgcacgagc tggaaaatgc gatgcatccg tatgaactgc
     3001 cgcaggtaca ttacaccgtt gtgcgcgtgg caaaacagca gatgggtgtc ggaggagacg
     3061 acagctgggg tgcacagacc catccggaat acctgattcc gattgataaa aaactggagt
     3121 ttacctttac gtttaaggga atctgatgcg ttcagcccat tctgtccgtg gacaggatgg
     3181 gctgtctgcg aaagaaaagg agacaaaagc agatggggaa agaagtaaat tttggaaagc
     3241 tcctgcacgg gggcgattat aacccggaac agtggctgga ttacccggag attctggaac
     3301 aggatctggc gtatttcaag aaagccagaa tcaacgaggt ttcgcttggc atgttttcct
     3361 gggcattttt agagccggag gaaggagttt tccgtctgga atggttaaaa gaggtgattg
     3421 accgcctgta cgaaaacgaa attgtggtta tgctttccac gccgacggcg gcaagaccgc
     3481 gctggatggc ggaaaaatac ccggaggtgc tccgtgtcaa tgcagcgaga gaacggaatc
     3541 tgtacgggga gcgccataat cactgctata cctcaccggt ttaccgggaa aaaacgagaa
     3601 tcatcaatac gaagctggcg gaaatgttca aagatcaccc tggcgtgatt gcatggcata
     3661 tttcgaatga atacggcgga gagtgccact gcccgctgtg ccaggaggca ttccgcggct
     3721 gggtgaagaa aaaatacggc acactggaac ggctgaaccg tgtctggaat accgggttct
     3781 ggagtcatac ttaccagagt tttgaccagg tggagagccc gtctccgaaa ggagattttt
     3841 cgctgcatgg actgaatctg gactggaaac ggtttgtgac ggatcagacc gcggattttg
     3901 taaaatggga aatcaaagcg ctgcgggacg ctggggcaga acagccgtct accatcaata
     3961 tgatgtataa ctttaccgga ctgaactact acaagtttgc cgatgtgatt gactttgtat
     4021 cctgggacaa ttatccgacc tggcataaag aggcggagac ggtaacagcg atggatacag
     4081 gtatgcagca tgacattatg cggagcattc agaaaaagcc gttttatctg atggagagct
     4141 gtccttccgc aacgaactgg cagtccgtca gcaagctgaa aaaaccgggc atgctgcagg
     4201 ccgcgtcact gcaggcagtt gcgcatgggt cggatagtgt gctgtatttc cagatgaggc
     4261 agagccgcgg tgcatccgaa aaattccatg gggccgtgat cgaccattat ggcggatcgg
     4321 atactagggt attccgtgaa gtgacgaaag tcggcgcagc tttggagaaa ctgcaggaag
     4381 tgaacggttc gcagaacccg gcggaagcag ccgtgattta tgatgtcgaa aaccgctggg
     4441 cggtggaaga tgcggcgggt ccgcggaatg caggggtttt ttataaagaa accgtggaaa
     4501 agccatatca ggcattccgg aaactgggca tcaatgtgga tgtgattgat gagacgtgca
     4561 gcctcgacgg ataccgtgtt gtggcagcgc cgatggttta tctgctgcgg gaaggatgga
     4621 cagagaaggt tcggaacttt gtgaaaaacg gcggaatttt tctgctgact tactggtccg
     4681 gcattgtgga tgagacggat ctctgttact taggcggaac accgcatgac ctgatggatg
     4741 tgatgggatt ccggagtatg gagattgacg gactgtacga tggcgaatgg aacgagggca
     4801 ttccggaacc ggaaaatccg ctgcatctgg aactggcata ccgctgcagt catctctgcg
     4861 agctggtaca gccatcgacg gcggaagtag tgatgacata cggaaaggat ttctatgacg
     4921 gcatgcctgc gatgacccgg aatgtgtacg gaaagggaaa agcgtatgcc gtttgtgcgg
     4981 actttgaaca gggactgtac gatgaagtct gccggaaact ggcagaagag gccggtgtgg
     5041 agcggatcgt ggaagaagtt ccggaaggtg tggaagtcac gatgcgtaag cagaaggacg
     5101 gaaccaggta cgtgtttgtc cagaacttta accgggaggc cgttgcggtc aaactgccga
     5161 tcgagcgcta tccggtatgg cagggaacgt atgacggaac gatcggaagc tggaagacga
     5221 ttgtgctgaa aggaagatag aagagaagaa aagtctgttg agaaaaatcg gaatgggctg
     5281 tttctcaaca gacttttttc gttcatacat ttgcggcagc gtcttcttcc tgcgccagct
     5341 ttttcttata cgcctcgccc cagtcccaca tcgcatccat gatcgggtgg aggctgaagc
     5401 cgagttccgt cagggaatat tcgacacgcg gcggtacctc ggcgtaaact ttacggttga
     5461 ccagaccgct ctgttccatc tggcggagct gggaagttaa aactttttga gagacgtgac
     5521 cgatggactt tttcagctcg ccgaaccgca tggtgccgtg gagcagcaga tcgcgcaaga
     5581 tcagaacttt ccatttatca ctgatcagag tcagcgtagt ttccaccggg cagggcggaa
     5641 gatcagcaag agaaacgggt gttttttctg cggatgacat aagagaaaac ctcctttttg
     5701 aagaaaataa tgatttgaaa gatggcggaa acgtctgatt ttacacaata gtatcttttg
     5761 ggtaactaca ccacaatatt gtgcttactt tacgaaagaa ctctatggat ttattataaa
     5821 gccatcgaaa gaaaaaaaca attcaaaaaa acaaaatgga ggaaagtaaa atgagcaaag
     5881 cagtagaatt tttaaatgag aatccgatac agtatctggc aacggtaggt cgtgacggaa
     5941 aggcaaaatg ccgcccgttc atgtttgcag gagaggttga cggaaaactg tggttctgca
     6001 ccaacaacac aaaagatgtt tacaaagata tgcaggagaa tccggaaatc gaaatctctg
     6061 tttccagccc gtcctacgca tggatccgtc tgcacggaac cgcagtgttt gaaaataaca
     6121 tggctgtaaa agaagtgtgc atgcagaacc cgatcgtaaa aggccagtac cagaccgctg
     6181 acaacccgat tttcgaggta ttctatctgg acaatgcaca cggtgtaa
//
LOCUS       DAOMUF010000120         6206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3413_length_6206_cov_4.703951, whole genome shotgun sequence.
ACCESSION   DAOMUF010000120 DAOMUF010000000
VERSION     DAOMUF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3413_length_6206_cov_4.703951"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..687)
                     /locus_tag="PLN77_07515"
     CDS             complement(274..687)
                     /locus_tag="PLN77_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44156.1"
                     /translation="MNCVYFYPKEYIEEADKRGLIDKDAVMKKGKQVMIPFCIILFVV
                     LIAIIFVWNKVTDFKTAYLQSYLFLVVMNWFDGIMLDRVWVGHSKIWEIKGMEGIPYI
                     KPWKTVLTKRGLATILYLIIALVTAGIVVLVGKIC"
     gene            complement(1040..1564)
                     /locus_tag="PLN77_07520"
     CDS             complement(1040..1564)
                     /locus_tag="PLN77_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM44157.1"
                     /translation="MNIKYNQLCIRNAEKKDCEQLAVWWNDGTVMAHAGFPNGLGTSA
                     AEIEKQIADDSDETRRRLIIEYNDVRIGEMSFYVFENHRYEIGIKICESDYQEKGIGR
                     VVLSMLIEELFRMGANVIFLDTNLKNTRAQHVYEKLGFRKTAVHYNSWKNQLGEMESS
                     VDYELTADEFCNLK"
     gene            complement(1590..2030)
                     /locus_tag="PLN77_07525"
     CDS             complement(1590..2030)
                     /locus_tag="PLN77_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM44158.1"
                     /translation="MTERIIDNELRLIPYYRNDEASLPWYQDLDVCRQVDNREKPYDV
                     ELLHRMYDYLTAHGDCYYIEYKGVLVGDVSLRENSEIAVVVCKEYQNQHIGRRCVLEM
                     LKLAREKGMERVKANIYSFNGQSQRMFQSVGFEKTAEEWYESKL"
     gene            complement(2058..2552)
                     /locus_tag="PLN77_07530"
     CDS             complement(2058..2552)
                     /locus_tag="PLN77_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM44159.1"
                     /translation="MKTLYLIGGTMGVGKTTVSQQLKKDLANSVFLDGDWCWDADPFQ
                     VTDETKEMVMDNICYLLNNFLHCSAYENVIFCWVMHEQSIVDEIVSKLDTEECRVIKI
                     SLIVDEANLRKRLLSDIANKIRTEEIMDKSIARIQMYQVLDTVKIDTSNKSVCEITEE
                     IAAL"
     gene            complement(2549..3109)
                     /locus_tag="PLN77_07535"
     CDS             complement(2549..3109)
                     /locus_tag="PLN77_07535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM44160.1"
                     /translation="MRHSIPEKGDMPNEQIPLSEEGKALAVSKRNIFSNVDRCFSSPY
                     RRAMETAEFIADHPVIVKELHERTIGDAREDFWLKQYEDHDFRNPGGESLNQVKVRMK
                     TAIDSIVGQMEEGETALVVSHATAICAYLLSYCEIEVKDAANKVRKISFHGKEILNDR
                     FQPAEGFEILFENDIFSDIRVMGREK"
     gene            complement(3458..4063)
                     /locus_tag="PLN77_07540"
     CDS             complement(3458..4063)
                     /locus_tag="PLN77_07540"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRM44161.1"
                     /translation="MAKLYFRYGAMGSSKSANILMVRYNYEERGQYAVLLKPRTDNRD
                     GEHEIKSRIGLEAPAEYVDEFLKEISETWSTDSGEYRYHGKKVDAVLIDEAQFLSPEE
                     VDTLSDIVDFYGIPVLCYGLRADFQTHLFPGSRRLMEIADIIEEVPTVCWCGRRAQCN
                     TRYADGKIVREGAQIFLGSNESYVALCRKHYKEGKLWKEDE"
     gene            complement(4088..4462)
                     /locus_tag="PLN77_07545"
     CDS             complement(4088..4462)
                     /locus_tag="PLN77_07545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM44162.1"
                     /translation="MMHVEAVDREGLRRRVKDPFYQNTTSILAMENDQVLGRIEYHFY
                     GCMQDGYRMAYVDWVYVLPEARHKGVAQGLFQEMEKDCRKHGINQYYLIRATNPNADK
                     FYHSFENAELDEEPFLRKELKE"
     gene            complement(4628..5578)
                     /locus_tag="PLN77_07550"
     CDS             complement(4628..5578)
                     /locus_tag="PLN77_07550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM44163.1"
                     /translation="MDYVIFDLEWNQCPYGKGKENPKLPFEIIEIGAVKMDGERNVVG
                     TFHRIVKPVVYQHLHHRTREVVGLTEEDLQNGIPFAQAVQEFFAFSGGACMFGTWGPG
                     DLTELQRNMKFYNLLSLIRGPIHFYDVQKLFAIQYETMKSRRALEYAADFLHLPKDEH
                     FHQALSDAKYTADIFARIDMDVVLAYDSIDVYQNPKSKKEEIHAVYNGYTKFISREFD
                     TKEEAMHDREVCSTHCCICGKTARKRLRWFSAGGKNYYCVAWCPEHGYLKGKARMKHS
                     DEGKLFVVKTIRISSEQEAGEIRQKKENLRKKRNQKRKEM"
     gene            5862..>6206
                     /locus_tag="PLN77_07555"
     CDS             5862..>6206
                     /locus_tag="PLN77_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM44164.1"
                     /translation="MKTKLTQKQIRFQALLLFFITFFLVELCAVFLYQNQLKEAKLKA
                     DYTAQTTIGRVKSQLNHYLAESNLMKHMIEAGYTVDDEEFSVLSSLMQDDQNVIKAHE
                     LAKDGIVTLIYPM"
BASE COUNT         1523 a         1645 c         1102 g         1936 t
ORIGIN      
        1 acaattcatc ccaccgcttt agaagcgggg gacttcttgt ctactgatgt taaaattctg
       61 cttccattat accgtctccg cccttcgttc tcaatgccaa aaatttttcg aatcaaattt
      121 ggtacgcctg cggcataaaa atatctctgt tttactgatt tgctccagta aaacagagat
      181 atttaacttc ctatatattc cgaaagtccc ttacgcaaaa atactactca tgagtagtat
      241 ataatattct tctctttttt tcaatgctgt ctcttaacag attttcccaa ccagcactac
      301 aatcccagct gtcacaagtg caatgatcag atacagaatc gtcgccaggc ctcgttttgt
      361 cagcacggtt ttccatggtt ttatgtacgg aatcccttcc attcctttga tctcccatat
      421 tttactgtgt ccaacccaca cccggtcaag cataattccg tcgaaccagt tcatcaccac
      481 aaggaagagg taggattgga gatatgccgt cttgaaatcg gtgactttat tccagacaaa
      541 aatgatcgcg atcagcacca cgaacaaaat gatgcaaaat ggaatcataa cctgctttcc
      601 ttttttcatg accgcatctt tgtcgatcag tccacgttta tcggcttctt ctatatattc
      661 ctttgggtaa aaataaacac agttcattcc atcatttctt accagacact ttaccatcag
      721 aataaacaga agacagtata atatgttttg caacaacagc atctcacatt cctcctcaga
      781 ctaccgttta tgtttcttat agcttcatgt tagttcaggc taatcacatt gtcaagagga
      841 cttgctgttt ttacattaac actgccgcaa tggataaatt ataatcgttt ataaagcaaa
      901 aaaagaaacg agcaagtcct gtcaggcata aaccttaccc agtgaaaacc catttctttt
      961 ttagacaatg tcaaagatac catacttctt tctcccatcg aactttcttt acactcgtcc
     1021 ctcatttatt ttacaatttc tatttcagat tgcaaaactc atccgccgtc agctcatagt
     1081 cgacagaaga ttccatttcc cccagctgat ttttccagga attgtaatgg actgctgttt
     1141 tgcggaagcc gagtttttcg tagacatgct gtgctcttgt gtttttgagg ttggtatcaa
     1201 gaaaaataac gttggctccc atgcggaaca attcttctat gagcatgctc agtacaactc
     1261 ttccaatgcc tttttcctgg taatcggatt cgcagatttt gattccgatt tcatatcgat
     1321 gattttcgaa tacatagaaa ctcatttcgc cgattcgcac atcgttgtat tctatgatga
     1381 gtcttcgtct tgtttcatcg ctgtcgtcgg caatctgttt ttcaatttcc gctgcgcttg
     1441 tcccaagccc gtttggaaat ccggcgtggg ccatgacggt tccatcgttc caccagacgg
     1501 ctaattgttc acaatctttc ttttcggcat ttctaatgca cagctgattg tattttatgt
     1561 tcatttcttc tctctttctc tgcaatcact tacagcttag actcatacca ttcctccgcc
     1621 gttttctcaa atccgaccga ctggaacatg cgctggcttt gtccattaaa agaataaata
     1681 ttggctttta cccgctccat tcccttttct cttgcaagct tcagcatctc cagaacgcac
     1741 cgtcttccaa tatgctgatt ctggtattct ttacagacaa cgacagcgat ttcactattc
     1801 tctctcagcg aaacatctcc caccagcacg cccttatatt ctatataata gcagtcaccg
     1861 tgcgcggtaa gataatcata catccgatgc aacagctcca catcatacgg cttctcccgg
     1921 ttgtccacct gcctacacac gtccagatcc tgataccacg gaagagaggc ctcgtcattc
     1981 ctgtaatatg ggatcagccg gagttcattg tctatgattc gttcggtcat tttggtttgg
     2041 ctccttgttt tttgatctta aagtgctgca atttcctctg taatttcaca gacactctta
     2101 ttactcgtat caatttttac cgtatcaagt acctgataca tctgaattcg tgcaatgctt
     2161 ttgtccataa tctcttccgt acgaatctta tttgcgatgt cggataataa ccgcttccgc
     2221 aaatttgctt catccacgat cagcgaaatt ttaatcactc tgcattcttc cgtgtccagc
     2281 ttactgacga tttcatcaac aatactctgc tcatgcatca cccagcagaa aataacgttt
     2341 tcataagcgc tgcaatgcag aaagttattc agcagataac agatgttgtc catcaccatc
     2401 tctttcgtct catccgtcac ctgaaacgga tcggcgtccc agcaccagtc gccgtcaaga
     2461 aaaacgctgt tcgccagatc ttttttcagc tgctgactta ccgtcgtctt gccaacgccc
     2521 atcgttccgc cgatcaaata taatgttttc atttttctct ccccataaca cgaatatccg
     2581 aaaaaatatc gttttcaaaa aggatttcaa acccctctgc cggctgaaat ctgtcgttca
     2641 ggatttcttt tccatggaag gatatttttc ttactttgtt tgccgcatcc ttcacctcga
     2701 tctcacaata cgaaagtaaa tatgcgcaga ttgccgttgc atgagaaacc acgagtgctg
     2761 tttctccttc ttccatctgt ccgacgatag aatcgatcgc ggttttcatc cgcaccttca
     2821 cttgatttaa cgattccccg cccggattgc gaaaatcgtg atcttcatac tgtttcagcc
     2881 agaaatcctc cctggcatcc ccgatcgttc tttcatgcag ctccttcaca atgaccgggt
     2941 gatctgcgat gaattcagcc gtttccattg ctctccgata aggtgaagaa aagcatctgt
     3001 ccacatttga aaatatattt ctttttgata cagcaagcgc ttttccctct tccgatagag
     3061 gaatctgctc attcggcata tccccttttt ccgggatgga atgcctcatt aaaaatattt
     3121 ttttcatttc actctcccct gctgttattc ctaaagtttt ttaatcaaaa acatctccat
     3181 cggctttttt ccatatcctt tttctgatgt tccccagcga taaaaagcat ggtgcaaaac
     3241 ggagtattat cccagaaaag attatattgc agcaatcaaa tgtttccagc ggacctgctc
     3301 ttgtggatat tcttgataaa ctgatacgaa tggatgttgt tgcaaaagaa gctcctatta
     3361 acgatgaaaa tgatgtgtcg cacggacgga agcttcccac agttccacca gcgtttccat
     3421 ccgcggcatt ctctcctgta cttcatatat tgttatctta ctcatcttcc ttccacagct
     3481 ttccttcttt ataatgtttc ctgcaaagcg ccacataact ctcgttcgat ccaaggaaaa
     3541 tctgcgcgcc ttcccgaacg atttttccgt ctgcgtatct cgtattgcac tgcgcccgtc
     3601 ttccgcacca gcagaccgtt gggacttcct cgatgatgtc agcgatttcc atcaatcttc
     3661 tcgaacccgg aaacagatgt gtctggaagt ccgcacgcag accgtagcag agaaccggaa
     3721 tcccataaaa atccacgatg tccgaaagcg tatcaacttc ctccggagaa agaaactgcg
     3781 cctcgtcgat aagaaccgca tccactttct ttccgtgata ccggtattcc ccgctgtctg
     3841 tactccaggt ctctgaaatt tcttttaaaa attcatccac atattccgcc ggtgcctcca
     3901 gcccgatgcg ggacttgatt tcatgttctc cgtcgcggtt gtccgtccgc ggcttcaaca
     3961 ggacagcata ctgcccgcgc tcctcataat tatagcgaac catcaaaata ttagcagatt
     4021 tcgaactgcc catcgcgccg tagcggaaat ataattttgc cattgttttt cgtccttttg
     4081 tcttttctta ttctttcagt tctttccgta aaaacggctc ctcatcaagc tctgcgtttt
     4141 caaagctgtg ataaaattta tctgcatttg gatttgtcgc ccgaatcaga taatattggt
     4201 taatcccatg tttcctgcag tctttttcca tctcctgaaa cagcccctgc gcaacgcctt
     4261 tatgtctggc ttccggcagc acataaaccc agtccacata cgccatccgg taaccgtcct
     4321 gcatacatcc gtaaaaatga tactcaatcc gcccaagaac ctgatcattc tccatagcaa
     4381 gaatggatgt tgtattctga tagaagggat cttttactct tcttcgcagc ccttcccgat
     4441 ccaccgcttc cacatgcatc atctccggct ctgcccccat ttcttttcta taacagaaaa
     4501 ctaccgacaa acctgctttt atcgatagtt tcttcaattt ttattataac cgatcccgct
     4561 ctgttttcca attaaaaagg cagaagaaat gaaacctttt atcccgtttc catttcctct
     4621 gcctttctta catttctttc ctcttctgat tcctcttctt ccgcaggttc tctttcttct
     4681 gccggatttc ccccgcctcc tgttcgctgc tgatgcggat ggttttcacg acgaagagtt
     4741 ttccttcgtc cgaatgtttc atgcgggctt tgcctttcaa atatccgtgc tccgggcacc
     4801 aggctacgca gtagtagttt ttcccgccgg ccgaaaacca gcgtaagcgt tttcttgccg
     4861 ttttcccgca gatgcagcag tgcgtgctgc agacctcgcg gtcgtgcatg gcttcttctt
     4921 ttgtgtcgaa ttcgcgggag atgaatttcg tgtagccatt atagacggcg tggatctcct
     4981 cttttttcga tttcgggttc tgatatacgt cgatcgagtc gtacgccagc acaacatcca
     5041 tatcgatgcg cgcaaaaatg tcggccgtat attttgcatc cgaaagcgcc tgatgaaaat
     5101 gctcgtcctt cgggagatgg aggaagtcgg cggcgtactc gagcgcccgg cggctcttca
     5161 tcgtttcgta ctggatcgcg aacagtttct gtacatcgta aaaatgaatc ggcccgcgga
     5221 tcagcgacag gaggttataa aatttcatgt tccgctggag ctccgtcagg tctcccggtc
     5281 cccaggtgcc gaacatgcac gcaccgccgg agaatgcaaa aaattcctgc acggcctgcg
     5341 caaatgggat tccgttctgc agatcctctt ctgtcagtcc gacgacctcg cgcgtccggt
     5401 gatggagatg ctggtagacc accggtttta caatccggtg aaacgttccc accacattcc
     5461 gctcaccatc catcttcacc gcgccgatct caataatctc aaacggcagc ttcgggttct
     5521 cttttccttt tccataggga cactggttcc attccaggtc aaaaatcaca tagtccattg
     5581 tattgtcaat ccttcattct gtgtcgtctg tccctattat aaatgatttt gatataatga
     5641 gcaactatct ggcaattcca ttctctgccc attctggcaa ttttcttcct gtgcattaca
     5701 tttcccattc atttcatcct gttctctttc aaacttaacg cttcttttat tgaaatccca
     5761 gggtaaatcc gatataatat ttttaactcc ggccttcgtt tttactcaaa aaaatcacct
     5821 gaaaaagccg gaaatattac gaatttagat gagcatccga tatgaaaaca aaattaacgc
     5881 aaaaacagat tcgatttcag gcgcttctgc tgttttttat tacatttttt cttgtcgagc
     5941 tctgcgccgt ttttctgtat cagaatcagt taaaggaagc aaagctgaaa gctgactata
     6001 ccgcacagac gaccatcggc cgcgtgaaat cgcagttaaa ccattatctt gccgaatcca
     6061 atctgatgaa acacatgatt gaagccggtt ataccgtcga tgacgaagag ttttccgtcc
     6121 tctcctcgct gatgcaggac gaccagaatg tcatcaaagc acatgagctt gcaaaggacg
     6181 gcattgtcac actgatttat ccgatg
//
LOCUS       DAOMUF010000121         6201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3419_length_6201_cov_4.412626, whole genome shotgun sequence.
ACCESSION   DAOMUF010000121 DAOMUF010000000
VERSION     DAOMUF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3419_length_6201_cov_4.412626"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1136)
                     /gene="trxB"
                     /locus_tag="PLN77_07560"
     CDS             complement(216..1136)
                     /gene="trxB"
                     /locus_tag="PLN77_07560"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRM44165.1"
                     /translation="MENLYDVIIIGSGPAGLSAAIYAKRAKLSSLTIEANFASGGQVL
                     NTYEVDNYPGLPGISGMDLGSTLRAHAEKMGAEFSRERVKELVLDEEIKIVRTRKNEY
                     HAKTVILATGAEHRALNVPGEKELSGMGVSYCATCDGAFFKDKTVAVIGGGDVAVEDA
                     IFLARTCKKVYVIHRRDELRAAGVLQDALLALPNVEMVWDTVVDSIEGNEMVSGVKVT
                     NKKAGEAGVITVDGVFIAVGIVPVSALIAGTVETDEAGYIKAGEDGVTSVPGVFAAGD
                     VRTKQLRQIITAAADGANCVTSVEKYLISH"
     gene            complement(1196..2521)
                     /locus_tag="PLN77_07565"
     CDS             complement(1196..2521)
                     /locus_tag="PLN77_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44166.1"
                     /translation="MKKYRWVLTAVFAAFLVGTSGCGKKTETIPITTISQSTDDDDPE
                     DNLAASGDSDEIPEYDVDLSKNLNSFQLAIWGDTYEIPESYADFTALGWVYSGDDAKE
                     IQPESFSEGEIFEKDGNQITVDIANPDTTAKPVAECLIGGIHIDTSTAEGQNIYVGLP
                     NGVTLQQSLMEDVESIYGAPKDRYEADTSVQFTYEYGLYQTITLGFDNETGILYSLDM
                     QNFTTTADAEALDGVSDATTPEVEAYQAPEADSSEINDWTVRFDDVLYHLPVPVSELL
                     DHDWTVNTKESDTAVLNGKYGYVTLEKGGQKLYCTVHNYGAEATTVRNCFVTSLYGDL
                     DTTKIPISITNGITLGTSESDFLAKAGDAKSEKTEKEDSNLTLYTFYSDDEKLDYTEI
                     GIDNDLKLVRSIKVVHNQPEAPEEEAKKTSAEDSSSVSDSQEPSETPAP"
     gene            2727..3575
                     /gene="dapF"
                     /locus_tag="PLN77_07570"
     CDS             2727..3575
                     /gene="dapF"
                     /locus_tag="PLN77_07570"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRM44167.1"
                     /translation="MRFTKMQGCGNDYIYVNAMEERVESPGRLAKAVSDRHFGIGADG
                     LILIGASKTADFSMEMYNADGSKGAMCGNGIRCVGKYVYEHGLTRKTTLTIETGSGNR
                     VIHLQTGENLNEICQVTVEMGTPEFRAAQIPVVSLGEYVLEHKITVCGENYTMTCLSM
                     GNPHAVIPVEDVEKISIAKTGSAFEHLSCFPDRINTEFIQTLDQHTIKMRVWERGSGE
                     TLSCGTGACAAAVAAMLSGCVSEGETAVELRGGRLFVTWDRTKNKVLLTGPAETVFEG
                     TLAERI"
     gene            3747..5882
                     /gene="nrdD"
                     /locus_tag="PLN77_07575"
     CDS             3747..5882
                     /gene="nrdD"
                     /locus_tag="PLN77_07575"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRM44168.1"
                     /translation="MVYIIKKDGTKEEFDAQKIVRAVNKSAQRILYTFSQQEIDFICK
                     FATEHVYSLGKTEIPISDMHNIVEGALEKVNPAVAKSYRDYRNYKTDFIHMMDEVYTK
                     SQSIRYIGDKSNANTDSALVATKRSLIFNELNKELYRKFFMNRNELQACKDGYIYIHD
                     QSARLDTMNCCLFDVGNVLKGGFEMGNVWYNEPKTLDTAFDVMGDIVLSTAAQQYGGF
                     TVPEVDKILAPYAQKSYDYYVKEFLKYTDASWEGAQEKAIEYAIDKVRRECDQGWQGI
                     EYKLNTVGSSRGDYPFVTVTLGLGTSMFEKMISISLLEVHKNGQGKKGHKKPVLFPKI
                     VFLYDKAIHGEGGIAEDVFEAGLDCSSKTMYPDWLSMSGEGYIAEMYKKYKRVISPMG
                     CRAFLSPWYERGGMEPADENDKPVFVGRFNIGAVSLHLPMILAKSRSENRDFHEVLDF
                     YLEMIRDLHKRTYDYLGEMRASTNPLAYCEGGFYGGHLKPSDKIRPLLKPMTASFGIT
                     ALNELQELYNGKSLVEDGQFALDTLKYINDKVAEFKKEDGILYAIYGTPAESLCGLQI
                     EQFRKQYGIVRNVSDRPYVSNSFHCHVTEDITPIQKQDLEGRFWELCNGGKIQYVRYP
                     VGYNKGAIKTLIERAMELGYYEGVNLSLAYCDDCGHQELDMDVCPVCGSKNLTKIDRM
                     NGYLSYSRVHGDTRLNEAKMAEIAERKSM"
     gene            complement(5980..>6201)
                     /locus_tag="PLN77_07580"
     CDS             complement(5980..>6201)
                     /locus_tag="PLN77_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44169.1"
                     /translation="CAGGDAKFDMQGNIGAMWCFSVPLGFLAAFWLKLPVMVVYCIVN
                     LDEIVKLPAVYLHYKKYVWVRNITRDIEE"
BASE COUNT         1557 a         1568 c         1615 g         1461 t
ORIGIN      
        1 ataacttgtg gacgagatgt ttataaaatt ggcccgtgtg ggaaaaaacc ggtttctttg
       61 tgcaatttcg acaaaaagag gttttaaagt tttacacgac tgacatgaaa ggtttttcag
      121 atttcagaaa ctacggaaaa acagaaaaaa gcaggcagtt ctccgtacaa tacgaagaat
      181 gcctgctttt gctgtattct attcgaaaga agccatcagt ggctgatcag atatttctct
      241 accgaagtca cacagttcgc gccgtcggcg gctgccgtga tgatctgacg gagctgcttc
      301 gtgcggacat cgcctgcggc gaacacgccc ggcacgctcg tcacaccgtc ttcgccggct
      361 ttgatgtagc cagcctcatc ggtttctacg gtgcctgcga tcagtgcgga gaccggaacg
      421 atgccgacgg caataaacac gccgtctacc gtgatcacac cggcttcccc tgcctttttg
      481 ttcgttacct tcacgccgga caccatctcg tttccctcga tggaatccac aaccgtatcc
      541 cacaccattt ccacattcgg cagtgccagc agtgcatcct ggagaacacc ggctgcccgc
      601 agttcatcgc ggcggtggat cacgtaaacc tttttgcacg ttctggcaag gaaaatcgca
      661 tcctccacgg cgacatcgcc gccgccgata acggcaacgg ttttgtcttt gaaaaatgcg
      721 ccgtcacacg ttgcacagta agagacgccc atgccggaga gctccttttc accaggtaca
      781 tttaacgcgc ggtgctccgc accggttgca agaatgaccg ttttggcatg atactcattt
      841 tttctcgtgc ggacaatctt gatctcttcg tcaagaacaa gctccttcac acgctcgcgg
      901 gaaaattccg cgcccatttt ttcagcgtgt gcgcgcagcg tgcttccgag atccatgccg
      961 ctgatgcccg gaaggccggg atagttgtct acttcatagg tattcagcac ctggccgccg
     1021 cttgcaaagt ttgcctcaat cgtaagagag gagagctttg cgcgtttcgc ataaatcgcg
     1081 gcagaaagac ccgccgggcc ggagccgatg atgataacgt cgtataagtt ttccataaaa
     1141 atcctcgttt tcgtctgtag taagctgcca tgcagccatt tttctgaaag tatcgttacg
     1201 gagccggtgt ttcggacggt tcctgactgt cggaaacgga agatgaatct tccgcggaag
     1261 tttttttggc ttcttcttcc ggcgcttccg gctggttgtg aaccaccttg atgctgcgta
     1321 ccagcttcag atcgttgtcg atgccgattt cagtgtagtc cagcttttcg tcatcactat
     1381 agaatgtgta gagtgtcagg ttactatcct ctttttccgt tttttctgat ttggcatccc
     1441 ctgccttcgc cagaaaatcg ctctcggaag tgccgagcgt gatgccgttt gtgattgaaa
     1501 tcggaatctt tgtcgtatcg agatcgccgt acagcgaagt aacgaaacag ttgcgcactg
     1561 tcgtcgcctc cgcgccgtaa ttatgtacgg tgcagtaaag cttctgaccg cccttttcca
     1621 gcgtgacgta gccgtatttt ccgtttaaaa cggccgtatc ggactctttc gtattgacgg
     1681 tccagtcgtg gtccagaagt tcactgaccg gaacgggaag atgatagagt acatcgtcaa
     1741 agcggacggt ccagtcgttg atttcactgc tgtcggcttc cggtgcctgg taggcctcca
     1801 cttccggtgt tgtggcatcg ctgacaccat cgagcgcttc tgcatcagcc gtggtcgtaa
     1861 agttctgcat atcgagggaa tacagaattc ccgtttcgtt atcgaagccg agcgtgatcg
     1921 tctggtacag cccgtactca tacgtgaact gcacagaggt atcggcctca taacggtcct
     1981 ttggcgcacc gtagatgctt tccacatcct ccatcagcga ctgctgcagg gtgacgccgt
     2041 tcggcaggcc gacatagata ttctggccct cagcggtcga ggtatcgatg tgaatgcctc
     2101 cgatcaggca ttccgccact ggctttgccg tggtgtccgg attggcaata tcgacggtaa
     2161 tctggtttcc gtccttttca aagatttcgc cctctgagaa ggattccggc tgaatttcct
     2221 ttgcgtcgtc gccgctgtag acccagccaa gcgctgtaaa atccgcgtag gattccggaa
     2281 tttcgtatgt atcgccccag atggcaagct gaaaactgtt caggttcttc gagagatcca
     2341 catcgtattc cggaatctca tcggaatcgc cggaggccgc cagattgtct tcggggtcgt
     2401 catcgtcggt actctgactg atggttgtga tcggaatcgt ttctgttttc tttccgcagc
     2461 cgctcgttcc cacgaggaaa gcggcaaata ctgctgttaa aacccaacgg tattttttca
     2521 tcaaaacctc ctcataataa atggttaaaa atatttgaaa gttacttcat gtaagtaacg
     2581 atatcagttc cccctattct atcacattcc gcttcgtttt ccaatgtctg aagcggattt
     2641 gagaaaaatg gtttttcaaa agcgcggtac ggaatacata tttagtaagg aactggaaag
     2701 ggcagaagtc tgggaggagg ctgtgtatgc gttttacaaa aatgcagggc tgcggaaatg
     2761 attatatcta tgtaaatgcc atggaggagc gggtggagag tccgggacgg ctggcaaaag
     2821 cggtaagtga ccgccatttc ggcatcggcg cggacgggct gattctgatc ggggcatcca
     2881 aaacagcaga tttttcgatg gaaatgtaca acgccgacgg ctcgaagggc gcgatgtgcg
     2941 gcaacggcat ccgctgtgtg ggaaaatacg tgtacgagca tgggctgacg agaaaaacga
     3001 cgctgactat cgaaaccggc agcggaaacc gtgtgattca tctgcagacg ggggagaatc
     3061 tgaatgagat ctgtcaggtg acagtggaga tgggaacgcc ggaattccgc gccgcacaga
     3121 tccccgttgt ttctctgggg gaatatgtgc tggagcataa aattaccgtg tgcggggaaa
     3181 attatacgat gacctgcctt tcgatgggaa atccgcatgc ggtgattccg gtggaagatg
     3241 tggaaaaaat atccattgca aagaccggtt ccgcttttga acacctttcc tgcttcccgg
     3301 accgtatcaa tacagagttt atccagactt tagatcaaca cacaataaaa atgagagtct
     3361 gggaacgtgg gagcggtgaa acgctgtcct gcggaacggg agcgtgtgcg gcagccgtcg
     3421 cggcgatgct ttccggctgt gtttcggagg gagaaactgc cgtagaactg cgcggcggca
     3481 ggcttttcgt tacatgggac cggacgaaaa ataaggtcct tctgaccggc ccggccgaga
     3541 ccgtttttga aggaacgctt gcagaacgaa tttgaaaaat gcagatttct gtctgcacga
     3601 aagagcagta tgcaatattc tgaaaataaa aacttacagg aaatgccttt tcttttcaaa
     3661 agaaacatgc tacaatatgt ggtacccgct acaagaaaag ggtgggggac acacaagata
     3721 tagtgtttga tttggaggac accacaatgg tttatattat caaaaaggac ggcaccaaag
     3781 aagaattcga cgcgcagaag atcgtccgtg ccgtaaataa atccgcacag agaatcctgt
     3841 ataccttctc acagcaggag attgacttta tctgtaagtt cgcaaccgag cacgtatatt
     3901 cccttggaaa gaccgaaatt ccgatcagcg atatgcacaa tatcgtggag ggagcactcg
     3961 aaaaagtaaa cccggctgtg gcaaaaagct accgcgatta ccgcaattac aaaactgatt
     4021 ttatccatat gatggacgag gtctacacga agagccagtc catccgctac atcggcgaca
     4081 agagcaatgc caacaccgac agcgccctcg ttgcaacgaa gcgaagcctg attttcaacg
     4141 agctgaacaa agagctgtac cgcaagttct tcatgaaccg caacgagctg caggcctgta
     4201 aggacggcta catctacatc cacgaccagt ccgcacgtct cgataccatg aactgctgtc
     4261 tgttcgatgt cggaaacgtt ttaaaaggcg gcttcgagat gggaaatgtc tggtacaacg
     4321 agccgaagac actcgacacc gcatttgatg tcatgggcga tatcgtcctc agcaccgctg
     4381 cacagcagta cggcggcttt accgtgccgg aggtggacaa gatccttgct ccgtacgcac
     4441 agaagagcta cgattactac gtaaaagagt tcctgaaata taccgacgca tcctgggagg
     4501 gtgcacagga gaaagccatc gagtatgcga tcgacaaagt aagaagagag tgcgatcagg
     4561 gatggcaggg aattgagtat aaactgaaca ctgttggatc ttcccgcggt gattatccgt
     4621 tcgttaccgt gaccctcggc cttggaacga gcatgttcga gaagatgatt tccatctccc
     4681 ttctcgaggt ccacaaaaac ggacagggca agaaaggcca caaaaagccg gtgctcttcc
     4741 cgaaaatcgt cttcttatat gacaaggcaa ttcacggaga gggcggcatc gcagaagatg
     4801 tcttcgaggc aggacttgac tgttcttcca agacgatgta tccggactgg ctgtcgatga
     4861 gcggcgaggg ctacatcgcc gagatgtaca agaaatacaa gagagtcatc agcccgatgg
     4921 gatgccgtgc tttccttagc ccgtggtacg agcgcggagg catggagccg gcagatgaga
     4981 acgacaaacc ggtctttgtc ggacgtttca acatcggagc cgtttccctt catcttccga
     5041 tgatcctggc aaaatcccgt tccgagaacc gcgatttcca cgaagtgctt gacttctacc
     5101 tcgagatgat ccgcgatctg cacaaacgga cctacgatta cctcggcgag atgcgtgcat
     5161 ccacgaaccc gctggcttac tgcgagggag gtttctacgg cggccactta aaaccgtccg
     5221 acaagatccg cccgctgtta aagccgatga ccgcatcgtt cggcatcacg gcgctgaacg
     5281 agctgcagga gctgtacaac ggaaaatctc ttgttgagga cggacagttc gcactcgaca
     5341 cgctgaaata tatcaacgac aaggttgccg agtttaagaa agaggacggc atcctctatg
     5401 cgatctacgg cacaccggca gaatccctgt gcggcctgca gatagagcag ttccgcaaac
     5461 agtacggcat cgtccgcaac gtttcggacc gcccgtatgt gagcaacagt ttccactgcc
     5521 acgtaaccga ggacatcacc ccgatccaga aacaggatct cgagggacgt ttctgggagc
     5581 tctgcaacgg cggaaaaatc cagtatgtgc gctacccggt cggctacaac aaaggtgcga
     5641 tcaagaccct gatcgagcgt gcaatggagc tcggctacta cgagggcgtc aacctgtccc
     5701 tggcttactg cgatgactgc ggccaccagg aactcgacat ggatgtctgc ccggtctgcg
     5761 gttccaagaa cctgacgaag attgaccgta tgaacggcta tctttcctac agccgtgtcc
     5821 atggcgacac ccgtctgaac gaggcgaaga tggcagaaat tgcggaaaga aaatcgatgt
     5881 aaaatcattg cgatatttgt aatacgataa aagaacgaaa tcccctgcgg gaaatccatt
     5941 cggtttggat tttccgcagg ggattttata gttcggtaat tattcttcga tatcgcgggt
     6001 gatgttgcgt acccagacgt attttttata atgaagataa accgcaggca gtttcacgat
     6061 ctcatcgagg tttacgatac agtagacaac catgaccggc agcttcagcc agaatgcggc
     6121 aagaaagcca agcggtacag aaaagcacca catagcgccg atgtttccct gcatatcaaa
     6181 tttcgcatcg cctccggcac a
//
LOCUS       DAOMUF010000122         6195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3425_length_6195_cov_3.437296, whole genome shotgun sequence.
ACCESSION   DAOMUF010000122 DAOMUF010000000
VERSION     DAOMUF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3425_length_6195_cov_3.437296"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="PLN77_07585"
     CDS             <1..1635
                     /locus_tag="PLN77_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRM44170.1"
                     /translation="IADADGEVQMTLGLGDIRVRAFDGIRRAEAMMRIEEEDSLELVL
                     REEAVPEADDRWQTLEIRAPKEVPVRNIVATKEQKERNAARMAEAERLRKARFAACFD
                     EKKAAAYPEAEEIFHRAGENFEEVYTFLSKDENPNRKKLLFSLALKDAKDLKASVLED
                     HLNCEQGDLPEEIFRKDLLCPRIFLEELTPYRSMIRGFFEEETKHSFAEQPERILDYL
                     KKNITFHAEEEYDTIMATPVGVLTMKQGSPLAQKILFVAICRSLNVAARLNPVTLEPE
                     YYHNGAFHSVETEAAVLEEETESAVLTLNAEDGSAWKYYQTWTIGKWNGTVFETLNYE
                     ETAFDGKTLDLTLEPGCYRLITSMRMPNGDQHAAYRVFELKAGEATKEIYLETVKKEL
                     DELLEHIELPEITLEDLDGKAHTLNDLTKDGPILLAFLGTGEEPTEHVLNELIEIAEK
                     WNAKDAAMAAVLRTKADLENTTFQKARAAIHNMSLYLDPSDDAPAIAEKMGIDAEKLP
                     LLILALPGNIGGRAYAGYNVGSVGLMLRLMEEAAKA"
     gene            complement(1684..2286)
                     /locus_tag="PLN77_07590"
     CDS             complement(1684..2286)
                     /locus_tag="PLN77_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM44171.1"
                     /translation="MIDTIIFDVGRVLVNWDYESYLKRFAFGPEKTKAIAAATFEGPY
                     WRQFDRGILTPEEIVKGFCSLAPEYTEEIKEVFAHCEETITPLPYAESWVRSLKEKGY
                     RLYILSNYSGDLFERTKEKMPFLPYMDGTLFSYTCHLTKPEPEIYQHLIQKFSLTPDR
                     CVFLDDTKANVEAAEQAGIHGIHFTGYEDGVAQLKKLGVL"
     gene            complement(2283..2519)
                     /locus_tag="PLN77_07595"
     CDS             complement(2283..2519)
                     /locus_tag="PLN77_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44172.1"
                     /translation="MKRLKQIGALLSIAVLIGFGLGAMIFAFIDTPFAHKALTVCLYC
                     AITAPVLIYAMMLVAKVLSHRDENDKSDKGEKQS"
     gene            2711..3112
                     /locus_tag="PLN77_07600"
     CDS             2711..3112
                     /locus_tag="PLN77_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44173.1"
                     /translation="MDKLKEKLSRLMYGRYGIDQLGNFMMWAAVAMMIFGAVVRSSLI
                     YTLALVLIVWADVRIMSKNHSKRYAENQKFLELKNRVVGFFRGGTRAFKDKEHCYFRC
                     PSCHQQVRVPKGKGHIEIRCPKCGTKFIKNT"
     gene            3134..3973
                     /locus_tag="PLN77_07605"
     CDS             3134..3973
                     /locus_tag="PLN77_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HRM44174.1"
                     /translation="MFGYVTIRKDDLKVKDYNKYQAYYCGLCQDIKEAYGKKGQAMLT
                     FDMTFLSILLTGLYECETKEEEHYCFLHPGKKHKCLRNKYTKYCADMNVLLAYYNLLD
                     DWEDEKNYAAFTAAKALKKSVTRIEASYPRQTKAVQDYLEKLHACEKENNPDLDLAAG
                     YTGEVMAEIYIPEEDIWSDDLRQVGFYIGKFIYLMDALDDVEKDKKSGNYNPFLSIAD
                     QPDFEETAEKILLMMTAEASRAFEKLPILESVDILRNILYAGIWEKYEMRKSAKERQK
                     EEK"
     gene            3980..4687
                     /locus_tag="PLN77_07610"
     CDS             3980..4687
                     /locus_tag="PLN77_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRM44175.1"
                     /translation="MTDPYEVLGVSRGASDDEIKKAYRKLSRTYHPDANINNPNKAEA
                     EEKFKQVQQAYKQIMDEREHGTSYQSGGSSYGGDAYGGYGPFGGFDPFGGAYGNGGSA
                     RNDYGNSEQEMRLQAAANYINSGHFREAMNVLNDISDRSGKWYFLHAIANSRLGNNIN
                     AVQDAEMAVKLEPQNLQYQQLLNQLQGSGQWYSDMGSGYGYERSAGDLGKWCCECMAI
                     NAICNCCCCGGRGFFCC"
     gene            4976..>6195
                     /locus_tag="PLN77_07615"
     CDS             4976..>6195
                     /locus_tag="PLN77_07615"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM44176.1"
                     /translation="MERKTAWNEYKKKDMKKLEKLNAGYRAFLDHGKTERECVKESVR
                     QAEEAGYVSLDTYVKENRALKPGDKVYAVCVKKAIALFQIGTKPLTEGMNILGAHIDS
                     PRLDVKQNPLYEDNGFTYLDTHYYGGIKKWQWVTLPLAIHGVVAKKDGEVVDVVIGED
                     DNDPVFCVTDLLIHLSREQQTKPASTVVEGENLDLLIGSQPLEGTEKDAVKAMTLKLL
                     NDKYGIEEEDFLSAELEIVPAGKARELGMDASMILAYGQDDRVCAYTSLVAMLEVEAP
                     EKTSCCLLVDKEEIGSVGATGMQSHFFENTMAELLALAGEGTELALRRCLASSRMLSS
                     DVSAAFDPLYAAAFEKKNAAFFGKGIVINKFTGAGGKSGSNDANAEYVGALRAIFDDA
                     KVNWQTAELGKVDVG"
BASE COUNT         1792 a         1367 c         1714 g         1322 t
ORIGIN      
        1 attgcggatg cggacggaga agttcagatg acacttggcc ttggcgatat ccgggtacgg
       61 gcatttgacg gaatacgccg ggcggaagcc atgatgcgga ttgaggagga ggattcgctg
      121 gaactggtgc ttcgtgaaga ggcggttccg gaagcggatg acaggtggca gacgctggaa
      181 atccgggcac cgaaggaagt gccggtacgg aatatcgttg ccacaaagga gcagaaggag
      241 cgaaatgcgg cacggatggc agaggcggaa cggcttcgga aggcacggtt tgcagcatgc
      301 tttgatgaga aaaaggctgc tgcgtatccg gaagcggaag agattttcca ccgggcagga
      361 gagaattttg aggaggttta tacattcctt tccaaagatg aaaatccgaa ccggaagaag
      421 ctgctgtttt cgctggcatt aaaggatgcg aaggacttga aggcatccgt tttagaggat
      481 catctcaact gtgagcaggg ggatctgccg gaggagattt tcaggaagga tcttctttgc
      541 ccgcgtattt tcctcgagga gctgacgcca tatcggagca tgatccgcgg attttttgag
      601 gaagagacaa aacacagctt tgcggagcag ccggaacgga ttctggatta tcttaagaag
      661 aatattacct tccatgcaga agaagagtat gacacgatca tggcaacacc ggtcggtgtc
      721 cttacgatga aacagggaag tcctctggcg cagaagattc tgtttgtggc aatctgccgg
      781 agcctcaatg tagcagcaag actgaatcca gtgaccctgg agccggagta ttatcacaat
      841 ggagccttcc acagtgtgga aacagaagct gctgtcttgg aagaggagac agaaagcgcg
      901 gttctgacac tgaatgcgga ggatggaagc gcatggaaat actatcagac gtggaccatc
      961 ggaaagtgga acggaacagt gtttgagacg ctgaattacg aagaaaccgc attcgacgga
     1021 aaaacactgg atctgaccct ggagccgggc tgctatcgtc tgattacaag tatgcgtatg
     1081 ccgaacggtg accagcatgc cgcataccgc gtgtttgaac tgaaggcagg agaagcaaca
     1141 aaagagattt atctggaaac cgtgaaaaaa gagctggatg agctgttaga gcacatcgaa
     1201 ctgccggaga tcacgctgga ggatctggat ggaaaggcgc ataccctgaa cgatctcacc
     1261 aaagacggcc cgatccttct ggccttcctc ggaaccggag aggagccgac cgagcatgtt
     1321 ctgaatgagc tgatcgaaat cgcagagaag tggaacgcaa aagacgctgc catggcagcc
     1381 gtgctccgca cgaaagccga cctggaaaac acgactttcc agaaagcccg cgcagcaatc
     1441 cacaacatgt ccctctacct cgacccatcc gacgacgccc cggcgatcgc cgaaaaaatg
     1501 ggaatcgatg cagaaaaact cccactgctg atcctcgcat tgccgggaaa catcggcgga
     1561 cgcgcttatg ccggatataa cgtcggcagc gtcggactga tgctgcggct gatggaagag
     1621 gcggcgaaag cgtaagttaa tactaaaaaa caggctgacg cacgaatgcg gcagcctgtt
     1681 tttttaaaga acgcccagtt ttttcaactg tgccacgcca tcttcatatc ccgtaaaatg
     1741 aattccatga atccctgcct gctctgcggc ctcgacgttt gctttggtgt cgtcgaggaa
     1801 tacgcagcgg tccggggtga gggagaattt ctggatcagg tgctggtaga tctccggttc
     1861 cggttttgtt aaatggcagg tgtaggagaa gagggttccg tccatgtagg ggaggaaggg
     1921 cattttctct ttggtgcgct cgaagaggtc tccggagtag ttggagagga tgtagaggcg
     1981 gtagcctttt tcttttaagg agcgtaccca gctttctgca tacggcagcg gggtgatggt
     2041 ttcctcgcag tgggcgaaaa cctctttgat ttcttcggtg tattccgggg cgagggagca
     2101 gaatcctttt acaatctcct ccggtgtgag aattccgcgg tcgaactggc gccagtaagg
     2161 gccttcgaag gttgcggcgg cgattgcttt cgtcttttcc ggaccgaaag caaagcgttt
     2221 cagataggac tcgtagtccc agttgacgag tacgcggcct acatcaaaaa taatggtatc
     2281 aatcatgact gtttttctcc tttgtctgat ttgtcgttct catcgcggtg cgacagaacc
     2341 ttggcaacca gcatcatggc atagatgagc actggtgcgg taattgcaca gtagaggcag
     2401 accgtcagcg ccttgtgtgc aaacggcgtg tcgataaacg caaaaatcat ggcgccaagc
     2461 ccgaatccga tcaggaccgc gatggagaga agggcgccga tctgtttgag tcgtttcata
     2521 aaaatctccg atcgtttact gaaaataaaa tataaagaaa cataaccaag tgtagcacag
     2581 ttgaaaagaa acatccaccg caagttttaa gaatttttta gaaaaaatta atggcgcttt
     2641 cgattgcttt tttccctcaa aagggataga gtattggcat atactttgaa aaaggagaaa
     2701 cgagaacaaa atggataaac tgaaagaaaa actttcccgc ctgatgtacg gccgctacgg
     2761 catcgatcag ctgggcaatt ttatgatgtg ggcggcagtt gccatgatga ttttcggagc
     2821 ggttgtacgg agctccctta tctacaccct ggcactcgtc ctgatcgtct gggcggatgt
     2881 gcgcatcatg tccaagaacc attccaaacg gtatgcggaa aaccagaaat tcctggaact
     2941 gaaaaaccgc gtcgtcggat tcttccgagg cggaacccgt gcattcaagg ataaagagca
     3001 ctgctacttc cgctgcccgt cctgccatca gcaggtgcgt gtgccgaaag gaaaaggcca
     3061 catcgagatc cgctgcccga aatgcggaac aaaattcatc aaaaacacct gataaaaagg
     3121 agcagtgcga ttcatgtttg gttatgtgac catccgaaag gatgacctga aagtcaaaga
     3181 ttacaacaaa tatcaggcat actactgcgg tctgtgtcag gatatcaaag aggcgtatgg
     3241 aaaaaagggt caggcgatgc tgacgtttga tatgacattt ctttccatcc tgctgaccgg
     3301 gctttatgag tgtgagacaa aagaagagga gcattactgc tttcttcatc ccggcaaaaa
     3361 acataaatgc ctgcggaaca aatacacaaa atactgcgca gacatgaacg tgctgctcgc
     3421 ctattacaat ctgctggatg actgggagga cgagaaaaac tacgcggcgt ttacagcggc
     3481 aaaggctttg aaaaaatctg tgacacgcat cgaggcatcg tatccgcgcc agacgaaagc
     3541 agtgcaggat tacctcgaaa agctgcatgc atgcgagaag gaaaacaatc cagacctcga
     3601 tcttgcggca ggctataccg gagaggtgat ggcggagatc tatattccgg aggaggacat
     3661 ctggagcgat gatctgcgcc aggtcggctt ttacatcgga aaattcattt acctgatgga
     3721 tgcgcttgat gacgtggaaa aagataaaaa gagcggaaac tacaacccgt ttctgtccat
     3781 cgcagatcag ccggacttcg aggaaaccgc agaaaaaatc ctgctgatga tgacggcgga
     3841 agcgtcgcgt gcatttgaaa aacttccgat cctggaaagt gtagacattc tgcgaaatat
     3901 attgtatgct ggtatctggg aaaaatacga aatgcgaaaa agcgcaaaag agcgccaaaa
     3961 agaggagaaa taaagactga tgacagatcc atatgaggtg ctgggcgtgt cccgcggggc
     4021 ttcggacgat gaaatcaaaa aagcataccg aaaactcagc cggacgtatc acccggatgc
     4081 caatatcaat aatccgaata aagcagaagc agaagaaaaa ttcaaacagg tgcagcaggc
     4141 gtacaaacag atcatggatg aacgcgagca cggcacctcg taccagagcg gaggttcgtc
     4201 gtatggcgga gatgcttacg gcggctatgg cccgttcggc ggcttcgacc cattcggcgg
     4261 cgcgtacgga aacggcggct ccgcacggaa tgattatgga aattccgagc aggaaatgcg
     4321 gctgcaggcg gcagccaact acatcaacag cggccacttc cgcgaggcga tgaacgtgct
     4381 gaacgacatt tccgaccgca gcggaaaatg gtatttcctg catgcgatcg caaattcgcg
     4441 cctcggcaac aacatcaatg ccgtgcagga tgccgagatg gcagtcaaat tagaaccgca
     4501 gaatctgcag taccagcagc ttttaaacca gctccagggc agcggccagt ggtattccga
     4561 catgggaagc ggttacggct atgagagaag tgcaggtgat ctcggaaaat ggtgctgtga
     4621 atgtatggca atcaatgcca tctgcaattg ctgttgctgc ggcggaagag gatttttctg
     4681 ctgttgatca aaaaataatt acaagaaatg tataaaagtg aacattcagg ggagaataac
     4741 agtgtcatcg aaattcccct gttcgatgat acaaaagaaa aataaggaaa tacttatcct
     4801 ccccttaaaa gccccgctgc aaccgaaaaa acggttcgct gtggggcttt tttctaacag
     4861 ccgaaagtcc ttgtttttcc catcaaaaca gattataatg aaagagatcg caattatgac
     4921 cggagcaaaa aaatacgaac ggatcataga aagaaaacag aaagagagaa aagctatgga
     4981 acgaaaaaca gcatggaatg agtacaagaa aaaagatatg aaaaagctgg aaaaattaaa
     5041 cgccggctac cgcgcattcc tggatcacgg aaaaacggag cgcgagtgtg taaaggaaag
     5101 tgtacgtcag gcagaagagg ccggatacgt aagcctcgac acctatgtaa aagaaaatcg
     5161 tgcgttaaaa ccgggtgaca aggtttatgc tgtatgcgtg aagaaggcca ttgccctgtt
     5221 ccagatcgga acaaagcctc tgacagaggg tatgaatatt ctcggtgcgc acatcgattc
     5281 tccgcgtctt gacgtaaaac agaatccact gtatgaggac aacggattta catatcttga
     5341 tacccattat tacggtggca tcaaaaaatg gcagtgggta acgcttccgc ttgcaatcca
     5401 cggtgttgta gccaagaagg atggcgaagt agtggatgta gtcatcggcg aggatgataa
     5461 cgatccggta ttctgtgtga cagatctcct gattcacctt tccagagagc agcagaccaa
     5521 accggcaagt acggtagtag agggcgagaa cctcgatctt ctgatcggaa gccagccgct
     5581 ggaaggaaca gagaaagatg cagtgaaagc catgacctta aagctcttaa acgacaagta
     5641 tggcatcgaa gaagaggatt tcctctccgc agagcttgaa attgtaccgg caggaaaagc
     5701 gcgtgagctt ggtatggatg ccagcatgat tcttgcatac ggccaggatg accgtgtatg
     5761 cgcatacact tctcttgtcg caatgctcga ggtggaagca ccggagaaga catcctgctg
     5821 cctgctcgta gacaaagagg agatcggaag cgtcggagca accggcatgc agtctcattt
     5881 cttcgaaaac accatggcag agctgcttgc acttgcaggt gagggaacgg agcttgcact
     5941 tcggagatgt ctggcttctt ccagaatgct ttcttccgat gtaagtgcag cgtttgatcc
     6001 gttgtatgca gcagcatttg agaagaaaaa cgctgcattt ttcggaaaag gtatcgtaat
     6061 taacaaattt acaggagcag gcggaaagag tggctcaaac gatgccaatg cagagtacgt
     6121 cggagctctt cgcgctatct ttgatgatgc aaaagtaaat tggcagacag cagagcttgg
     6181 taaagttgat gtcgg
//
LOCUS       DAOMUF010000123         6157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3450_length_6157_cov_4.039004, whole genome shotgun sequence.
ACCESSION   DAOMUF010000123 DAOMUF010000000
VERSION     DAOMUF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_6157_cov_4.039004"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..201)
                     /locus_tag="PLN77_07620"
                     /pseudo
     CDS             complement(1..201)
                     /locus_tag="PLN77_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(333..1364)
                     /locus_tag="PLN77_07625"
     CDS             complement(333..1364)
                     /locus_tag="PLN77_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44177.1"
                     /translation="MEKQGTVENQLAHTIDQTEYDSRYDRTAKKLLANKQILAQIMKG
                     CVNEYSDCTVDDIVEKYIEGTPEVGSVGVHVDDTNRPKKSTDVIKGSNNEDSTLTEGT
                     LFYDVRFDAIAPKSADSAEQEEVIRLIINVEAQTKFKPGYPLTKRAIYYCSRMISAQH
                     GPIFTKSEYGKIRKVYSIWICTQPSDGFENTLTRYSIKPEQLIGEAQEETENYDLMSV
                     VMICLGKPGTENHKGILKFMEVLLSSTRSGSEKKKILEEEFGVAMSEELEREVLEVCN
                     LSQGVRAEGREEGRQEGRIEGIGIGEIRMLVQLVREGDLPLERAAAKAKMTVEQFKET
                     MENTPLQAV"
     gene            complement(1560..1955)
                     /locus_tag="PLN77_07630"
     CDS             complement(1560..1955)
                     /locus_tag="PLN77_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44178.1"
                     /translation="MQKTEQKTEQQAGLGMSFCEEEGRITVFRKTLEALEWPSHYRFL
                     YNRKMKQIAVQACGAEESGAHRVGKPDESNSGEIKCMALVRMIYGDSGWDKKMSYRLL
                     GRAFPQQRLVSFKITDAMQIENGRVLTEM"
     gene            complement(2007..2708)
                     /locus_tag="PLN77_07635"
     CDS             complement(2007..2708)
                     /locus_tag="PLN77_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44179.1"
                     /translation="MNEENTTIMPPEKQIVQDDRDARAEELESAFSYDGYQVVRKELF
                     AHLRDPAIVIRKDSITFNTACITGLEDVVYVHVMFNNDLKRIVVRGCDENDKDALRWC
                     VAKPDKRKSRKMSCKPFATLVYQKMGWDSECRYKMLGYRITFEGETLYVFDLLVPEIF
                     HEGQRKKNAVDSQDNAASTKPVNSRKGFYLDDIVGTFGVPVEEHRKESEVKQMDGYVS
                     MGILTGKIAPEAGKD"
     gene            complement(2705..4543)
                     /locus_tag="PLN77_07640"
     CDS             complement(2705..4543)
                     /locus_tag="PLN77_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM44180.1"
                     /translation="MITGEVEAVDRKQKIRDRYKGVDISELEVIPAKIIEDLETSSVI
                     RRVAAYIRVSTDNDEQTSSYELQKNYYTDYIQAQPGWVFVGIYADEGISGTSLEHRKG
                     MQQLIEDCKAGKIDLVLTKSIARFARNIVDCLSVIELLKNLNPPVGVKFEADNIYTLD
                     SNGRMILTILASVAEEESHSKSIIMNWSIDRRFSRGLFLTPALLGYDKDEEGNLVINP
                     EEAQTVKVIYYLYLNGYSLTEIATLLMEYSRKTKLGRVEWNPGTLAGVLANERHCGDV
                     LARKTFTPNFLTHKSKKNNNDRTQYRQKNHHEAIVSREVFNAANHLRASRNYSKKNRP
                     LPVLSVVEDGILRGYVPFDKDWTGFSAEEYREASESVMKEPDVTVTADVKKRLDLTGY
                     EIVRVQYFSTMQNPAMTISNGRLRFNTACLKKFENVEYVELLLNSVERCIAIRPCDKN
                     NPNAIRWGRLKEGRWCASTLGCRGLAKTLFDIMEWDEDLRYRFRGQFLEQGDNKMMLF
                     AFDEPEMIKVEEIVLPPKENTEEDEGETVKKKIYIFPPEWAGTFGQPITSIAQVGILR
                     QEHYAGNWDVFRPATEIEEMNIFTAESLNELLREAEKIMEGWTDYR"
     gene            complement(4554..5531)
                     /locus_tag="PLN77_07645"
     CDS             complement(4554..5531)
                     /locus_tag="PLN77_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM44181.1"
                     /translation="MADKVEKRILTLYSDVQATSVHWLWYPFIAVGKITLLQGDPGDG
                     KSTMMMNLIAELSKGGKTPDGKPVGMPQRIIYQCSEDGVSDTIKPRLERCGADCRKVA
                     FINEETYSGLTLDDERIRQAIIEFRPRLVVIDPIQAYLGSDSDLQIAGRARKLMQRLG
                     MWASVYDCAIVLIGHLNKKEGTKGLYRSLGSIDVVAAARSVLQVERDQKDTDIRIVRQ
                     IKNSLAPSDGEIRFSITAEMGFQWLECKSTFVSSEQPKVPEFESKTEKAAYLIKKLLS
                     DGDMRAREIYMRMKDEGISRRTAENTKKELGIRSYRKMRQWYWSIHTGE"
     gene            5782..6123
                     /locus_tag="PLN77_07650"
     CDS             5782..6123
                     /locus_tag="PLN77_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM44182.1"
                     /translation="MATDYKALGKRISNARKQAGITQEALGEQLNMTRKHISVIESAI
                     KRPSLDALVDIANALDVSTDDLLVDSLIHSTSTSNSEIHRLLLDCNEIEQEILTRMVR
                     EMKAILYGLGI"
BASE COUNT         1542 a         1657 c         1110 g         1848 t
ORIGIN      
        1 aatgaacttg gatgcaacgc ttccgcgcca cttctcgcga tcccagtcca aaccgtggat
       61 agtgcagatt acacgcttgc caaaaagttt tggtatccaa cagaaaaagg caggaccttc
      121 tgcatggata tgcaccacat cgtatcttcc aaatgcacta caaagtgctg cgaaaaagga
      181 agagcttacc gctgcaagtc cccctgatgt caagataggg acaaaaaaat tctcaaaaaa
      241 agcaaaaaaa taagccccac aaggcttttc tgacaattct ttgaatcatc agggccttgt
      301 agggctatcc gttatcttat gctattttat tttcacactg cctgtaatgg ggtgttctcc
      361 attgtttcct taaactgctc aactgtcatc tttgcttttg ccgcagcacg ctccagcggt
      421 aaatcaccct ctcgaacaag ctgaaccagc atccgaatct cgccaattcc gatgccttcg
      481 attctgcctt cctgacggcc ttcttcacgg ccttcggctc ttacgccttg actaagattg
      541 cacacttcaa gcacctctct ttccagttct tcgctcattg caacgccaaa ctcttcttcc
      601 agaatcttct ttttctccga acctgatctt gtggaagata acagcacttc cataaatttc
      661 aggattccct tatgattctc cgtccctggc ttgcctaaac aaatcattac gacactcatc
      721 aaatcgtagt tttcggtttc ttcctgcgct tctccaatca actgttcggg tttgatggag
      781 taccgggtaa gcgtattttc aaaaccatct gacggctgag tacaaatcca gatggagtag
      841 accttacgaa tcttcccata ttcagatttt gtaaaaatcg gaccgtgctg tgcagaaatc
      901 attctgctac aataatagat tgcccgtttc gtcaatgggt atcccggctt gaattttgtc
      961 tgtgcttcca cattgataat cagacgaatg acttcttcct gttctgcact gtccgcagat
     1021 ttcggtgcga ttgcgtcaaa gcgaacatcg tagaacaatg ttccctcggt cagcgtagag
     1081 tcctcgttgt tactaccctt gattacatct gttgactttt tgggtctgtt cgtatcatcg
     1141 acatgaacac caacagagcc aacctccggg gttccctcga tgtatttctc aacaatatca
     1201 tctacggtgc aatcgctgta ttcattcaca cagcctttca tgatctgtgc cagaatctgt
     1261 ttgtttgcca acagcttttt cgctgttctg tcatacctcg aatcatactc tgtctgatct
     1321 atcgtgtggg caagctgatt ttccaccgtg ccttgttttt ccatcggcat tcacttcctc
     1381 gcagtagatt caacaagagc gtaaactctc ctatcgtact tccatcttac cataattcac
     1441 cgcccatatc aagcaaccaa ctgtaaactt tccttgcacg gagttgtcca tattattgca
     1501 ttcttcgcca ctgttatatc ataggcaact aattatttca gaacccacta atgtgagatt
     1561 cacatttcag tcagtactct cccgttttct atctgcatcg catccgttat cttaaacgaa
     1621 acaagtcgct gctgtgggaa agctcttccg agcagccgat aggacatttt cttgtcccac
     1681 ccagaatccc cgtaaatcat gcgaaccaat gccatacact tgatttcgcc gctattagat
     1741 tcgtctggtt ttcccacacg atgcgctcct gattcttctg ctccacacgc ctgtacagct
     1801 atctgtttca ttttacgatt atacagaaac cgatagtgtg acggccattc taacgcttcc
     1861 agcgtttttc tgaagaccgt gattcgtccc tcttcctcgc agaaactcat gccaagtcct
     1921 gcctgttgct ccgttttctg ttctgttttt tgcatattgc tcacctccca caaatggaca
     1981 gaccctcccc ttgtacaacc tgtcaattag tcctttccag cttcaggagc aatctttcct
     2041 gtcagaatcc ccattgacac atacccatcc atttgcttga cttcagattc tttccggtgt
     2101 tcttccacag gcacaccaaa agttcccaca atatcatcca ggtaaaatcc ctttcggctg
     2161 ttcacaggtt ttgttgacgc agcattatcc tgcgaatcga ctgcgttctt ttttctctgt
     2221 ccttcatgaa aaatttctgg cacaagcaga tcaaaaacat acagagtttc accctcaaag
     2281 gtaatcctgt accccagcat tttatatcgg cactcgctgt cccatcccat tttctgataa
     2341 actaaagtcg caaatggctt gcaggacatc ttccggcttt tgcgcttatc cggttttgcc
     2401 acgcaccaac gcagagcgtc cttgtcattt tcatcacacc cacgaacaac gatacgcttc
     2461 aaatcattgt tgaacatgac atgaacgtag accacatcct ccagccctgt gatacaggct
     2521 gtattgaatg tgatactatc tttacgaatc acgattgccg ggtcacgcag atgggcaaac
     2581 aattcctttc gcacgacctg gtagccatca tacgaaaagg cactttccag ttcttccgcc
     2641 cgtgcgtctc tatcatcctg cacaatctgc ttctctggcg gcatgattgt tgtgttttct
     2701 tcattcatct ataatcagtc catccttcca ttatcttctc cgcttcacgg agcaactcat
     2761 ttagactttc tgccgtaaat atgttcattt cctctatctc cgtcgcagga cgaaatacat
     2821 cccagtttcc ggcataatgc tcctgccgta agatacccac ctgtgctata cttgtaatcg
     2881 gctgtccaaa tgttccagcc cactctggtg gaaaaatgta aatcttcttt ttgaccgttt
     2941 cgccctcatc ttcttcggtg ttttcctttg gaggcagaac gatttcttcc accttaatca
     3001 tttcaggctc atcaaatgcg aacagcatca ttttgttatc gccctgctcc aaaaactggc
     3061 cacggaaccg ataacgcaaa tcctcatccc attccataat gtcaaataaa gtctttgcaa
     3121 gtccacgaca gcccaaagtg cttgcacacc aacgtccctc tttcagtctg ccccaacgga
     3181 tcgcgtttgg attattcttg tcacaggggc gaatggcaat gcagcgttca acagaattta
     3241 gcagcagctc cacatattca acattttcaa actttttcaa gcaagcggtg ttaaagcgca
     3301 atcgtccatt agaaatcgtc atagccggat tctgcatagt agaaaaatac tgtaccctga
     3361 ctatttcata gcccgtcaga tcaagacgct ttttcacatc cgcagtaaca gtgacatccg
     3421 gttctttcat cacgctttca gaggcttccc tgtattcttc tgccgaaaaa cctgtccaat
     3481 ccttatcaaa aggcacatat cctcgcaaaa tcccatcttc aaccacgctc aacacgggca
     3541 agggtctgtt tttctttgaa taattgcggg atgcccgcag atgattggct gcattaaaaa
     3601 cttctctgga aacaatggct tcatggtgat tcttctgcct gtactgtgtc cgatcattgt
     3661 tgtttttctt tgatttatgc gtcaaaaagt tcggtgtaaa ggtctttctt gccagtacat
     3721 caccacaatg gcgctcattt gccagaacac ctgcaagtgt accgggattc cactccacac
     3781 gtcccaactt cgtcttacgg ctatactcca tcaaaagagt tgcaatctcc gtaagcgaat
     3841 atccattcag gtacaggtag taaatcacct ttaccgtctg tgcttcctcc gggttaatta
     3901 caaggttgcc ttcctcgtct ttatcgtatc caagcaatgc aggggtcagg aacaggccac
     3961 ggctgaaccg ccgatcaatc gaccagttca taatgataga tttggaatgc gattcttcct
     4021 ctgccacaga tgccagaatc gtcagaatca tgcgtccatt gctgtccagc gtatagatat
     4081 tatctgcttc aaatttcaca cccacaggcg gattcagatt tttcagaagt tcaatcacag
     4141 aaaggcagtc aacgatattt cgagcaaaac gggcaatgga ttttgtaaga acaaggtcaa
     4201 tcttgcctgc cttgcagtcc tcaatcaact gctgcatccc ttttcggtgt tccagagatg
     4261 tgccgctgat gccctcatcc gcatagattc caacaaatac ccatcccggc tgtgcctgaa
     4321 tataatccgt ataatagttt ttctgaagtt catacgaaga agtctgttca tcattatccg
     4381 tggaaacacg gatatatgct gccacacgcc gaatcacaga acttgtttcc aaatcctcta
     4441 tgattttggc gggaatgact tccaattccg atatatctac gcccttatag cggtctctga
     4501 ttttttgctt acggtctact gcttctactt caccggtaat catgtgcttt tcctcattct
     4561 cccgtatgga tgctccaata ccactgtcgc atcttccgat aacttcggat accgagttct
     4621 ttcttcgtat tttctgcggt tctgcggctg attccttcat ctttcatccg catatagatt
     4681 tctcttgctc tcatatcgcc atcagaaagc aactttttta tcagatatgc tgccttttct
     4741 gtttttgact caaattccgg cactttcggt tgttccgatg aaacgaatgt gctcttgcac
     4801 tccaaccatt gaaaacccat ctccgctgtt atagaaaacc gaatttctcc atcagacgga
     4861 gccaaactgt tttttatctg ccgaacaata cgaatatctg tatctttctg gtcacgctcc
     4921 acctgcaaaa cacttcgcgc agcagcaacc acatctatgc tgccaaggct tcggtacaaa
     4981 cctttcgttc cctctttctt attgagatga ccaatcagca caatggcaca atcatatact
     5041 gaagcccaca ttccgagacg ctgcatcagt tttctggctc tccctgcaat ctggaggtca
     5101 gaatcacttc ctaaatacgc ctgaatcggg tctataacta caagccgtgg gcgaaattca
     5161 atgattgcct gacggatacg ctcatcatcc agtgttagac cactgtatgt ttcttcattg
     5221 ataaaagcta cttttctaca atctgcgcca catctttcca gtctgggttt aatggtatct
     5281 gatactccat cttccgaaca ctggtagata atcctttgcg gcatcccaac aggctttcca
     5341 tccggtgttt tcccaccctt agaaagttcc gcaatcagat tcatcatcat ggttgacttt
     5401 ccatcgccgg gatcgccttg tagcaatgtt atctttccca ctgcaatgaa tggataccac
     5461 aaccagtgaa cagaagttgc ctgcacatca ctatataatg taagaattct tttttctacc
     5521 ttgtccgcca tcgtctttcc ttcctatttg cagtcttttc ctacctctat tataaagcat
     5581 tcgttgcgtt ttgactgcta cccatcaggt agcatgagtt gccttttgct acccagtagg
     5641 tagcaaaaca gcctaagacc acagagcagc tagcagtgtg tccctttgcg gtcttagaac
     5701 ctttgatata gagttgctct gcctaccatc atgcgagata attgtactgc cttatgcggg
     5761 gaatcataag gagaaaagca catggctact gattataaag ctttgggcaa gcgcatttca
     5821 aatgcccgaa aacaagctgg tatcacgcaa gaggcccttg gagagcagct taacatgaca
     5881 cgaaaacata ttagcgtaat cgaatccgct atcaaacgcc caagtcttga tgcactggtt
     5941 gatattgcca atgctcttga cgtatcgact gatgatttgc tggtagatag tttgatacac
     6001 tcgacttcaa cctcaaattc cgaaattcac cgtcttctct tggactgcaa tgaaatcgaa
     6061 caggaaattc tcactcggat ggtaagagag atgaaagcca tactgtacgg tttgggaatc
     6121 tgattttgta acccgttgac catataacaa aaaatcc
//
LOCUS       DAOMUF010000124         6148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3458_length_6148_cov_3.603644, whole genome shotgun sequence.
ACCESSION   DAOMUF010000124 DAOMUF010000000
VERSION     DAOMUF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_6148_cov_3.603644"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PLN77_07655"
     CDS             complement(<1..1344)
                     /locus_tag="PLN77_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM44183.1"
                     /translation="MKKKVLAGILAATMAFSMAACGSTGSSSDSGSSAASGSTAKTEE
                     KGEPYTVTMVLQGSQQQDEERIEEKINEILEPELNAKLDIVVLPWASASQQLQLMLSG
                     DEKIDLLYTNATTAVQYMHSGQIMDMSELIDKYGTNLKDIYGEDIAKTNQIDGFVYGV
                     PNQIERGSIPAVFMRKDLVEKYNINTDEIKEPKDMEKVFETVQAGEPDMTMLFSSNNS
                     DTPLSRLSRADGLGDANTGALMDQTNSTTVENYFASDWYKETATMLHDWYQKGYISKD
                     AGTNTENWRTVCKAGNLFSLFFAYHPGTPVEFQSSTGYEFEIVPFYDQPIINSSSYGG
                     IIFSVAQNSENPEKAMQVLDYIYGSPEVMNLLNWGEEGTDYVVEDEENGIINYPDGVT
                     ADNAGYSFNCGWELPNQFIAYKWDGSDPQLWDKMQKFNASGIESKALGFVFDNSEY"
     gene            complement(1487..3277)
                     /locus_tag="PLN77_07660"
     CDS             complement(1487..3277)
                     /locus_tag="PLN77_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44184.1"
                     /translation="MQEEFKEKSKDAPFESLRILSERWKNGTFSEIIDDWKWIFGYSA
                     RYKGAIVFYTILGILSTSLGLVGSVAGKYLIDIITGYQIQKLPLLLCIMIGSTVFSLG
                     FESVINRISTKLGIAINNDIQADIFDKIVDADWLEISKYANGDVLNRFNGDIGTVSGN
                     AISWLPTIIIAVYRFIATFFVILHYDWVMALIAFLSAPFLLLMSRFMIRRQREYSKEV
                     REMSSNLMSFEVEAFYNFDTIKSFGIAPYYSKKMRWWQGLFKDISLKYNLFTIKTNIV
                     MSILGSAVEFTAFGYCLFRLWTHDITYGTMTLFLQQRSNLSGAFGNVISIIPSFLNSS
                     VSAHRIRELVELPKEVHIPESAKLDPLAKDGFRVQMHGVEFSYIEGNKVIIKSEFQAA
                     PGEIVALVGPSGEGKTTMIRLILGLIRPQEGETVIIASNGKTVPMNAETRHLFAYVPQ
                     GNTILSGTIAENMRMVKEDATDEEIIEALKISCAWDFVQHLPDTINSRVGERGRGFSE
                     GQSQRLAIARAVLRDAPVLLLDEATSALDVTTERNVLRNIIRQRPNKTCIVTTHRPSV
                     LGLCQRVYRVMNGTVTELDGAEGAKMVEDF"
     gene            complement(3249..4565)
                     /locus_tag="PLN77_07665"
     CDS             complement(3249..4565)
                     /locus_tag="PLN77_07665"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM44185.1"
                     /translation="MITETETAFLAVIRAFLTEEKASPAEIQGLTEKQWNHLFVLAAQ
                     HSLLSAVYDVVGKTPEFAELPDELRRQVKTQAMQSVLQQVSRTALFLPDEKELEHLGV
                     QPLVMKGIVCRSLYPKPDLRPSGDEDLLISAKDFATVDACFMRKGFVRDKETAMPNGA
                     KDGDTPEEMGYIHPKTGVFYEIHTRLFSTESSAYGYLNRAFSDVFAHPSKVEVQGQSI
                     FTLEETHHLFYLLCHSFKHFLHGGVGIRQICDMVQMIRVYGRKIDWEMFWQLCEEYHM
                     TCFCINLLDIGERYLGFSYEASGAVRAAKKLHPDSEALLIDILDAGSFGKSSAGRIHS
                     ANITLYAAETGAEKHTAGGIWKSLVPEASYMKNKYPYAARYPFLLPAAYMQRILKWLG
                     QGEKEQKSSIEVGAGRVELLRKYDMIRTADGKKRGDAHAGRIQREK"
     gene            complement(4562..5053)
                     /locus_tag="PLN77_07670"
     CDS             complement(4562..5053)
                     /locus_tag="PLN77_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HRM44186.1"
                     /translation="MKMMDTRAYVGMLKELVEQGREVRMVISGSSMAPFLIHGRDSIF
                     FRAPARELRKGDIVFYERPGGQFVVHRICRVHADGYDIIGDAQTEIEHHVQRKQIFAR
                     VTKVERKGKILVPGTFWWEFFAQVWIRIIPLRPILMHGYARLFGVDKKKNDACRENER
                     KRR"
     gene            complement(5050..5973)
                     /locus_tag="PLN77_07675"
     CDS             complement(5050..5973)
                     /locus_tag="PLN77_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44187.1"
                     /translation="MEKTFQTGSFSFRLKFPEELVLPVDMEKFLVETDSADYTYIITA
                     VDILPEAEGELIVDRVDLQVFHTKEGLESRRIGVKGEARPYAFYRETAQNRAEVFVGR
                     WYLAEVEKYEVVFLSLLALERRLAEQPCLILHCAYTEYQGKAMLFSAPSGTGKTTQAG
                     LWEQYRGSRTVNGDKALLEYDGTAWTANGWPVCGTSGVCENKKLPVGCIVMLSQAKTN
                     QAWRLRPAEAFTSLYGQITMNRWDREGLVHSLDLLEQLAGKVPVYHLACDISEDAVKT
                     LEQEIGIGSFECETYAAALHKNMIQKREKHA"
BASE COUNT         1394 a         1674 c         1376 g         1704 t
ORIGIN      
        1 atattcgctg ttatcaaaga caaatcccag tgctttcgac tcgataccgg aagcgttgaa
       61 cttctgcatt ttgtcccaga gctgcgggtc agatccatcc catttgtatg cgatgaactg
      121 gttcggaagc tcccatccac agttaaagct gtagcctgca ttgtctgctg ttacaccgtc
      181 cgggtagttg atgattccgt tttcttcatc ttctactacg tagtcggttc cctcttcgcc
      241 ccagttcaga aggttcatga cctccgggct gccatagatg taatcgagaa cctgcattgc
      301 tttttccgga ttttcggagt tctgtgctac agagaagata attccgccgt aagaggatga
      361 gttgatgatc ggctgatcat agaacggtac gatttcaaac tcatatcctg tggagctctg
      421 gaactcaacc ggggtacctg gatgataagc aaagaacaag gagaagagat ttcctgcttt
      481 gcatacggtt ctccagtttt ctgtgtttgt tccggcatcc ttgctgatat agcctttctg
      541 ataccagtca tgcagcatag ttgcagtctc cttgtaccag tcgcttgcaa agtagttttc
      601 tacggttgtg ctgttggtct gatccatcag agcgcccgtg ttggcatcgc caagtccatc
      661 agcgcgggac agacggctca gaggcgtatc actgttgttg ctggagaaca gcattgtcat
      721 atcaggctcg cctgcctgta cggtctcaaa tactttctcc atatccttcg gctctttgat
      781 ttcatcggta ttgatattat acttttctac caggtctttt ctcatgaaga cggctggaat
      841 gctgcctcgt tcgatctggt tcggaacgcc atagacaaac ccatcaatct gatttgtttt
      901 tgcaatatcc tcgccataga tatctttcag gtttgtgccg tatttgtcaa tcagttcaga
      961 catatccatg atctggccgg aatgcatata ctgtacagct gttgttgcat ttgtgtacag
     1021 aagatcgatt ttctcatcgc cggaaagcat cagctgtaac tgctgagatg cgcttgccca
     1081 cggaagaaca acgatatcga gctttgcatt cagctccggc tccagaatct cgttgatttt
     1141 ttcttcgatt ctctcttcat cctgctgctg gctgccctgc agtaccatag ttacagtgta
     1201 cggctcgcct ttttcctctg tctttgctgt gcttccggaa gctgcggagg aaccggaatc
     1261 agaactggat cctgtgcttc cgcatgctgc catggaaaat gccatagttg ctgcaagaat
     1321 gcctgctaac acttttttct tcatactttt tctccttttc acgtagcgtg ttgtgtgacc
     1381 tttgatggtt gtattatagc aggattttga cttacaactc tataactgat ttgactttga
     1441 agtatcactc ttccgacctt tgtgtctttt ttgtgtcttt tttctgtcaa aaatcttcca
     1501 ccatctttgc cccttctgcc ccatcgagtt cggtcacggt tccattcatg acacggtaga
     1561 ctctctggca gaggccgagg acactcggac ggtgcgtcgt aacgatacag gttttgttgg
     1621 gacgctgacg gatgatgttt cggaggacgt ttcgttccgt cgtgacatcc agtgccgagg
     1681 tggcttcatc cagaagcagc accggggcat cgcggaggac ggcacgggcg atggcaagcc
     1741 gctgggactg gccttcggag aaaccgcggc cacgttcccc gacgcggctg ttgatggtat
     1801 ccggcagatg ctgaacaaaa tcccaggcgc aggagatttt gagcgcttcg atgatctcct
     1861 cgtcggtcgc atcctctttt accatgcgca tattttcggc gattgtgccg gaaaggatcg
     1921 tatttccctg cggaacatag gcgaagagat gacgcgtttc ggcgttcatg ggaaccgttt
     1981 ttccgtttga ggcaatgatc acggtttccc cctcctgcgg gcggataagg ccgagaatca
     2041 ggcggatcat cgttgttttt ccctcgccgg acgggccaac caacgctaca atctcgccgg
     2101 gggcggcctg aaactccgat ttgatgatta ctttgtttcc ttctatataa gaaaattcta
     2161 ccccatgcat ctgtacccgg aagccatcct ttgccagtgg atcgagcttg gcgctctccg
     2221 ggatatggac ctcttttggc agctctacga gctcacggat ccggtgcgcc gagacggagc
     2281 tgttgaggaa ggacggaata atcgaaatca cgtttccaaa cgcgccggaa aggttactcc
     2341 gctgctgcag gaacagcgtc atggttccat aggtaatgtc gtgcgtccac agacggaaca
     2401 ggcagtatcc gaacgccgta aactccaccg cactcccgag aatagacatt acgatattgg
     2461 ttttaatcgt aaacagatta tatttcaggc tgatatcctt gaaaagtccc tgccaccagc
     2521 gcatcttttt actgtaatac ggtgcgatgc caaacgactt gatcgtatca aaattgtaga
     2581 acgcttctac ctcaaagctc atcagattac tgctcatctc gcgtacttct ttgctgtatt
     2641 cccgctgacg gcggatcata aaccgcgaca tcagaagcag gaacggtgcg cttaaaaaag
     2701 caatcagtgc catgacccag tcataatgca ggattacgaa aaaggtggca ataaaccgat
     2761 acacggcaat gataatcgtc gggagccagc tgattgcgtt gccgctgacc gttccaatgt
     2821 cgccgttgaa acggttgagt acgtcaccgt ttgcgtattt gctgatttcc agccagtccg
     2881 catcaacgat tttgtcaaaa atatccgcct ggatgtcgtt gttgatggcg ataccaagct
     2941 tcgttgaaat gcggttgatg acactctcaa acccaaggct gaacacggtg cttccgatca
     3001 tgatgcaaag cagcagcggg agcttctgta tctggtatcc ggtgatgata tcgatcaggt
     3061 attttcctgc cacgctgccg acaagcccaa gcgaagtgct taagataccg agaatcgtat
     3121 aaaaaacaat cgcaccctta tagcgcgcgc tgtagccaaa aatccatttc cagtcatcga
     3181 tgatttccga gaacgttccg tttttccatc gctccgacag gatgcggaga gactcgaagg
     3241 gagcgtcttt acttttctct ttgaattctt cctgcatgcg cgtctcctct ctttttcccg
     3301 tctgcggtac ggatcatatc gtatttcctc agcagctcga cacgtcccgc acccacttca
     3361 atgctcgatt tctgttcttt ttctccctgc cccagccatt tcaggattcg ctgcatgtat
     3421 gcagccggga gcagaaacgg atacctcgcc gcatagggat acttgttttt catataggaa
     3481 gcttccggaa ccagcgattt ccagattccc cctgctgtgt gcttttccgc cccggtctct
     3541 gccgcataca gggtaatgtt tgcactgtgg atccgtcccg cgctcgattt tccgaacgaa
     3601 ccggcatcga gaatatcgat cagaagcgct tcggaatccg ggtgaagctt ctttgcggca
     3661 cggactgccc cacttgcttc ataggaaaag cccagatacc gctcgccgat atccagcaga
     3721 ttgatacaga aacacgtcat gtgatactct tcgcacagct gccagaacat ttcccagtct
     3781 atttttcttc catatacacg aatcatctgt accatgtcac agatctgacg gatcccgaca
     3841 ccgccgtgaa gaaagtgctt gaagctgtga cagagaagat agaaaagatg atgcgtttct
     3901 tctaaggtaa agatgctctg cccctgcact tccactttgg atggatgcgc aaaaacatcg
     3961 gaaaatgcac gatttagata accataggca gaggattctg tgctgaacag cctggtatgt
     4021 atttcataga agacccctgt tttcggatga atgtagccca tctcttccgg ggtatcacca
     4081 tctttggcac catttggcat tgcggtttct ttgtcccgca caaagccctt cctcataaaa
     4141 caggcatcta ctgttgcaaa atcctttgcc gaaatcagaa gatcctcatc cccggacggg
     4201 cgcagatctg gtttcgggta gagacttctg cacacaattc ctttcatcac cagcggctgc
     4261 acgcccagat gctccagctc cttttcatcg ggaagaaaca gcgctgtacg cgaaacctgc
     4321 tgcaaaacac tctgcattgc ctgcgttttt acctgtctgc gaagctcgtc cggaagctct
     4381 gcaaattccg gtgtttttcc cacaacatcg tagaccgctg acagcagcga atgctgtgcc
     4441 gcgaggacaa acagatgatt ccattgtttt tctgtcagcc cctgaatttc cgccggcgat
     4501 gctttttctt ctgtcaaaaa cgcacgaatc accgcaagga atgcggtttc tgtttctgta
     4561 atcatcttct tttcctttca ttttctctgc acgcatcatt tttcttttta tccacaccga
     4621 ataaccgggc atatccatgc atcagaatcg gacgaagcgg gataatgcga atccagacct
     4681 gtgcaaaaaa ctcccaccag aacgttcccg gtacaagtat ttttcctttt cgttccactt
     4741 ttgtcacccg tgcaaagatc tgcttccgtt gcacatgatg ctcgatttcc gtctgtgcat
     4801 ccccgattat gtcataaccg tctgcatgaa cgcggcagat ccgatgcacg acaaactgtc
     4861 cgcccggccg ctcgtaaaat acgatatcac ctttccgaag ttcccgcgcc ggcgctcgga
     4921 agaaaatact gtcgcgtcca tgaatcagaa acggcgccat gctgctcccg gaaatcacca
     4981 tacgcacttc tctgccctgc tctacaagtt ctttcagcat tccgacatag gcacgtgtat
     5041 ccatcatttt catgcatgtt tctccctttt ctgaatcatg tttttgtgca gtgcagctgc
     5101 atatgtttca cattcgaacg aaccgatccc gatctcctgc tccagcgttt tcaccgcatc
     5161 ttctgaaatg tcgcacgcaa gatgatacac cggaactttt ccagcaagct gttcgagaag
     5221 gtcgagactg tgcaccagtc cctccctgtc ccagcggttc atggtgatct gtccgtacag
     5281 actcgtaaat gcctctgccg gacgcagccg ccacgcctga ttcgtctttg cctggctcag
     5341 cattacaatg catccgaccg ggagcttttt gttttcgcag actcctgatg ttccgcagac
     5401 cggccagccg tttgctgtcc acgctgtgcc gtcatattcc agcaatgcct tatccccatt
     5461 tacggtacgg cttccccggt actgttccca gagtcccgcc tgtgtggttt tgcccgttcc
     5521 cgatggtgca gaaaagagca ttgctttccc ctgatactcc gtgtatgcac agtgcagaat
     5581 caggcacggc tgctctgcca gacggcgctc cagggcaagg agggaaagaa aaacaacttc
     5641 atacttttca acttctgcca gataccacct gccgacgaaa acctccgcac gattttgcgc
     5701 agtctcacgg tagaatgcat aaggacgtgc ttctcctttc acaccgatcc ggcggctctc
     5761 caatccttct tttgtatgaa acacctgcag atcgacccga tccacaatca gctctccctc
     5821 cgcttccggc aatatgtcga cggccgtaat gatatatgta taatccgcag aatccgtttc
     5881 caccaaaaat ttttccatat ccaccggaag taccagctct tccggaaact tcagccggaa
     5941 ggaaaaactc cctgtctgaa atgttttttc catcaaaaac tcctctttct ccccatgatc
     6001 ttcccttctc tccgcaacgt atcacgatcc ctttttccgc atttatgata acaaaaaaaa
     6061 gagactccgt caatcttcag agtctctttt gtaattaaat acataatttt attttcgact
     6121 gatctgcagc gttttatagc catgccgg
//
LOCUS       DAOMUF010000125         6138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3462_length_6138_cov_2.619760, whole genome shotgun sequence.
ACCESSION   DAOMUF010000125 DAOMUF010000000
VERSION     DAOMUF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3462_length_6138_cov_2.619760"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLN77_07680"
     CDS             complement(<1..932)
                     /locus_tag="PLN77_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRM44188.1"
                     /translation="MGEYDRTGNGQSSKTDWDLRSILCDIAKNWWVILLIACSAAMIT
                     YTVMTTVHKDRYTVKTTFAVMGRGVDANAASSLSATYEMAQKFEAILENTILKKKVME
                     ELSLSSFPAKMNASIVPESNLMELSVTADSPEMAFRILKSVLNNYTSISDYIIPNVVL
                     ETLQQPQVSGVPSNQIPTKKYAATAFLAAALAMAALIGLFSFLRDTVKNEAEFTRKID
                     ADLLGVVYHEKKKKNSSMLITNPARSFLYVESLKRIASRVRGRLDRKGGKVLLVTSVA
                     ENEGKSTLAANLALALAEEQNRVLLLDCDFRQPA"
     gene            complement(944..1819)
                     /locus_tag="PLN77_07685"
     CDS             complement(944..1819)
                     /locus_tag="PLN77_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44189.1"
                     /translation="MHKTRAAVLILFLASTLAFGGCLVRQQTGKDGKGPEITMDNSEI
                     SASIHAEESELLAGVTAYDKKDGDVTSSLAVEHISNFVEKGRRKISIVAFDSDNHVTH
                     AERELVYNDYTSPVFSLDRPLRFSLNADDLTEGLSAEDCLDGTITDRIRISYEDEISS
                     TPGLYHVTYSVANRAGDVTSLPVTVELYDPAEENGRPQITLSDYLIHLDLQQPFDPQA
                     YLESVSVDQMLYEKREDGALHAASYEEELLGENQFSIDNPVDTGSAGVYEVTYTATAE
                     DGQTSSVRLIVCVNE"
     gene            complement(1865..2653)
                     /locus_tag="PLN77_07690"
     CDS             complement(1865..2653)
                     /locus_tag="PLN77_07690"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44190.1"
                     /translation="MTDLHVHILPGVDDGSPNLSTSLEMAEMAAQSGVRILAVTPHAN
                     QTGIEGVEDGYVNYESEQLEEQFYRLEREINREHIPISLVRGMEIMSIGPLEKKIRER
                     KLIPLHESRYYLIEVPFDMAPDGIRKRLLEFPAMGKIPVLAHPERYFCVQDTPELLYE
                     FREMGAVLQINKGSVFGRFGEEAERTARFLLENRLAGCVASDAHGADYRTTDMRPVRQ
                     FLEERYGEAYAQLLVKVNPRRILEDRQIFYEPSPERKRKRRWFL"
     assembly_gap    2757..2766
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2767..3726)
                     /locus_tag="PLN77_07695"
                     /pseudo
     CDS             complement(2767..3726)
                     /locus_tag="PLN77_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            complement(3795..4247)
                     /locus_tag="PLN77_07700"
     CDS             complement(3795..4247)
                     /locus_tag="PLN77_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44191.1"
                     /translation="MNREVETIREKVHTTADAALFLCFQEVKGSHSKGVIRLPLLHYD
                     IPFEGYGEMLLRMNRIYDLLGMPDAGSCMRSLSEGEKEEWEFTDEMKNNQDTTLPAGT
                     ERMVSIQIQFRQHCSLQGFLICGKQKAGFRSALEMLYLLEDCLQEQDN"
     gene            4687..5094
                     /locus_tag="PLN77_07705"
     CDS             4687..5094
                     /locus_tag="PLN77_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HRM44192.1"
                     /translation="MYSKLIFPLDPNDSVLLEIPVIISQGVSQKYRLALDDLIYVEAC
                     QHRLILHTIQGDFTTRCTFADLTVCLTPSDRFFHGGKGLLINFAHVSLVQVTGEVLLK
                     NGQIIFCSRRRRRETREAFLVYAGAGKFSYETI"
     gene            complement(5198..5860)
                     /locus_tag="PLN77_07710"
     CDS             complement(5198..5860)
                     /locus_tag="PLN77_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRM44193.1"
                     /translation="MERNVEIDKISDGKRYGANDLVKVGCDDCRGCSACCHGMGDSIV
                     LDPMDVYRLEKKLGKTMEEILLAGNVALRVVDGVILPHLKMTEQSDRCSFLNEEGRCS
                     IHDARPGFCRMFPLGRLYEDGTFSYFLQVNECPKENKTKVKVRRWLDTPELGKYEAFT
                     TKWHYYLKEKQNAARESEDDAFRQQISMNILKLFYLLPYDGNTDFYTQFAARMELAAD
                     KH"
     gene            complement(6017..>6138)
                     /locus_tag="PLN77_07715"
     CDS             complement(6017..>6138)
                     /locus_tag="PLN77_07715"
                     /inference="COORDINATES: protein motif:HMM:NF037804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44194.1"
                     /translation="DPRPSYQNSEERVYGLSFAGMEIKFQVKDEVLHVVGVEM"
BASE COUNT         1298 a         1702 c         1277 g         1851 t
ORIGIN      
        1 aacgccggct gacggaaatc gcagtccagc agaagtacac ggttctgctc ttctgcaagg
       61 gcaagcgcca gatttgccgc aagggtcgat tttccttcat tttccgccac actggtcacc
      121 agcagcactt ttccgccttt tcggtccaga cgcccccgca cacgcgatgc aatacgcttt
      181 aaggattcca catacagaaa gctccgcgcc ggattggtaa tcagcatgga gctgtttttc
      241 ttcttctttt cgtgatacac aacgcccagc aggtccgcat cgattttgcg ggtgaattct
      301 gcctcatttt tgacggtatc ccgcagaaag gaaaagagtc cgatgagtgc cgccatagca
      361 agggctgccg caagaaatgc ggtcgcggca tactttttgg tcggaatctg gttcgatggc
      421 acgccggaaa cctgcggctg ctgcagtgtc tccaacacta cattcggaat aatatagtcg
      481 gaaatgctcg tataattgtt cagtaccgac tttaagatcc gaaatgccat ttccggcgaa
      541 tctgcggtca cagacagctc catcagattg gattccggca caatggaagc attcattttt
      601 gccggaaacg aggacaggct aagctcctcc atcacttttt tcttcaaaat cgtattttcc
      661 agaattgctt caaatttctg tgccatctca tatgtggccg acaggctcga tgccgcattg
      721 gcatccaccc cgcgtcccat aacggcaaat gtggttttta ccgtataccg gtctttgtgt
      781 acggtcgtca tcaccgtgta ggttatcatc gccgccgaac aggcaatcag aaggatgacc
      841 caccagtttt ttgcaatgtc gcacaggata cttcggagat cccagtctgt tttgctgctc
      901 tgcccgtttc ctgttctgtc gtattctccc atctggatcc cccctattcg tttacacaga
      961 cgatcaggcg gacggagctt gtctgcccat cctccgctgt cgcagtgtat gtcacttcat
     1021 aaacacccgc actgccggta tcgaccggat tgtcaatgga aaactgattt tctcccagaa
     1081 gctcttcttc atacgaagct gcatgaagcg caccgtcttc tctcttttca tacagcatct
     1141 gatccacact cacgctttcc agatatgcct gcggatcaaa tggctgctgc agatccagat
     1201 gaatcaggta atcgctcagg gtaatctgcg gccttccgtt ctcctctgcc ggatcgtaga
     1261 gttctactgt caccggaaga cttgtcacat ctccggcgcg gtttgcaacc gaatatgtca
     1321 cgtggtacag tcccggtgta ctgctgatct catcctcgta agaaatccgg atgcggtcgg
     1381 taatcgttcc gtccaggcag tcctccgcgg aaagtccctc tgtcagatca tctgcattca
     1441 ggctgaaccg cagcgggcga tccagggaaa agaccggtga ggtgtaatcg ttatacacca
     1501 gttcccgctc cgcatgcgta acgtggttat cggaatcaaa ggcgacgata ctgattttcc
     1561 gccgcccttt ttccacaaaa ttcgaaatat gttccacagc aagagaactc gtcacatcgc
     1621 cgtctttttt atcgtaagca gttactccgg ccagaagttc tgactcctct gcatggatgc
     1681 tggcagaaat ctcactgttg tccatcgtaa tttccggccc ttttccgtct tttcccgtct
     1741 gctgccgcac cagacacccg ccaaaggcaa gtgtggaagc cagaaacaga atgagaactg
     1801 ccgctctcgt tttgtgcata tcaaagatgc tcctttcgtt tctgtaacgc cattcttcct
     1861 gtttttagag gaaccagcgt cttttccgtt ttcgttccgg ggacggttca tagaaaatct
     1921 gacggtcctc caggattctt ctcggattca ctttcactaa aagctgtgca tacgcttctc
     1981 cataccgctc ctccagaaac tgccggactg gccgcatgtc cgtggtccgg taatcggctc
     2041 cgtgggcatc gcttgccaca cagcccgcaa ggcggttttc cagtagaaac cttgctgtcc
     2101 gctcagcctc ttctccaaat cttccaaaca cgcttccttt gttgatctgc aaaaccgcgc
     2161 ccatttcccg gaattcgtat agaagctccg gtgtatcctg cacgcagaaa taccgctccg
     2221 gatgcgccag caccgggatt tttcccatcg ccggaaattc cagcagccgc tttctgattc
     2281 cgtccggcgc catatcaaac ggcacctcga tcagataata gcggctctca tggagcggta
     2341 tcagctttct ctctctgatt tttttctcca gcggtccgat gctcatgatt tccatgccgc
     2401 gcaccaggct gatgggaatg tgttctctgt tgatttccct ctccagacgg taaaattgct
     2461 cttctaactg ttccgattcg tagttgacgt atccgtcctc cacgccttcg attcccgtct
     2521 ggtttgcatg cggcgtcacc gcgaggatac gcaccccgct ttgtgccgcc atttctgcca
     2581 tttcaaggga ggtggacaaa ttcggggatc cgtcgtccac tcccggcaga atatgtacat
     2641 gaaggtccgt catccctgtt tttctccttc gtactggtat tccggtacca ttttctttac
     2701 cagataacag atgtccggac attcgttttc cgcttccttc gcgagttcct ttatctnnnn
     2761 nnnnnngaat accttctcgt caaatgcaat ctgtctgccg ataaaaatcc ggctgttttc
     2821 tgtggtctgc atgccctctt cattcatcag aagttcctca tacagctttt cgccgggtcg
     2881 caggccaatg tatttgatct tgatttcttt ttccggctca tatccggaaa ggcggatcat
     2941 atttttggcc aggtctgcga ttctgaccgg atctcccata ttcaggacaa agatctgacc
     3001 gtttttcgca taggcgcctg cctcaagtac aaggctgact gcctccggaa tcgtcataaa
     3061 atatcgaatg atatccggat gcgtaaccgt caccggaccg ccgtgttcga tctgacggcg
     3121 gaacagcgga atgacacttc cgttgcttcc cagtacattt ccaaagcgca ctgccgcaaa
     3181 ctctgtcgtg ctgtgtcttc cgatcagctg aatcaccatc tcacagagcc gcttgctcgc
     3241 tcccatgatg ttggtcgggt tgactgcctt gtctgttgat atcaggataa acttctttac
     3301 ctgataatgg cttgctgtgc gcgccgtttt taaggttcca ataacattgt ttttaatcgc
     3361 ttcattcggg ctgtcctcca tcagcggcac atgcttgtgg gctgctgcgt gaaagacgat
     3421 gtccggccgg tacttcgaaa aaattgattc cagcctccgc tcatcccgca cagaaccaat
     3481 cagtactgtc aaattgagat ctgggaactc ccattccaga gatttctgaa tctggtacgc
     3541 attgttttcg tagatgtcaa aaatgatcag atgcccgacg tcgtgtccgg ccagctggcg
     3601 gcagatctca cttccgatcg aaccgcctcc gcctgttacc aggattgttt ttcccttcac
     3661 atagcccagg attgcatcga gatccacccg gatgggatct cttggcaaca aatcctcaat
     3721 ttttattttc tttctgatct ttttttcttc cttcatagct cccactctca ttctgttgcg
     3781 tttttctccc gtattcagtt atcctgttcc tgcagacaat cttccagtaa atacagcatt
     3841 tcaagtgctg aacgaaaacc tgctttctgt tttccgcaga tcagaaatcc ctgcagacta
     3901 cagtgctgtc ggaactgaat ctgaatactg accatacgct cggttccggc tggaagcgtt
     3961 gtatcctgat tgttcttcat ttcatctgta aattcccact cttctttttc cccttctgaa
     4021 aggctccgca tacagcttcc tgcatccggc atgccgagca ggtcataaat gcggttcatc
     4081 cgaagcagca tttctccgta tccctcaaac ggaatgtcat aatgaagcag cggcagccgg
     4141 atcactcctt tggaatggct tcccttcact tcctgaaagc ataaaaataa tgctgcatct
     4201 gctgttgtat gtactttttc tctgattgtt tctacttctc tgttcatatt tctctccata
     4261 tgatatttat tcttttatgg taatgttata tgtaataatt atttttggaa tattatacca
     4321 aatcaatctc gcatcgtcaa tcttctctgc ctccttttct ttcgattttc ttttgatttt
     4381 cctgtttttt tcttctgctt tttctgtcta tcgttccttt tttttatctt tttttacttt
     4441 tttcgcccct ttctgccata cggtgtcact ttctgtcgaa cgattttaac agaagtccgt
     4501 caattcggct tgaatgagat tctgtcactg tgttaaacta cactttgtat ttttatgatg
     4561 tttgctgtaa tgctctcctc ctgcttttat tttgtaccct atcatgtttc tttgcgccgg
     4621 acaaccgctt cgacttgaac ggctgcactt cgacgcaaac ggtatacttt cccggaggtg
     4681 tttttcttgt attccaaatt aatattccca ctcgatccga acgattctgt tctgctcgaa
     4741 attccagtca ttatctcaca gggtgtttcc caaaaatacc ggctggcgct cgacgacctg
     4801 atttacgttg aagcctgcca gcaccgactg attctccaca ccattcaggg agattttaca
     4861 acccgctgca catttgccga cctgacggtc tgccttacac caagtgatcg ttttttccac
     4921 ggcggaaaag gtctgctgat caactttgcc cacgtttccc ttgtacaggt aactggcgaa
     4981 gttctgctga aaaatgggca gatcattttc tgcagccgcc ggcggaggcg tgagacccgg
     5041 gaagccttcc tggtctacgc cggagcagga aagttttcct acgaaacgat ataaaaaaac
     5101 aggagggctg ccgcacgaag aaaaatttcc acaaaatata gattttgtag tgttttcgct
     5161 tcgtgcgaca gcctctcctg ttaatatatg tttttgctta atgcttgtct gctgcaagct
     5221 ccattcgagc tgcaaactgt gtataaaaat ctgtatttcc gtcatacgga agcagataaa
     5281 acagcttcag aatgttcatg ctgatctgct gccggaatgc atcgtcctcg ctttcgcgcg
     5341 ccgcattctg tttttccttc agataataat gccattttgt cgtaaaggct tcgtatttcc
     5401 caagctccgg cgtatcgagc cagcggcgga ccttgacctt tgtcttgttc tccttcggac
     5461 attcgttcac ctgaaggaaa taagaaaatg ttccatcctc atacagccga cccagcggaa
     5521 acatccgaca gaagcccggg cgtgcatcgt ggatgctgca gcggccttct tcatttaaaa
     5581 agctgcaccg gtctgactgc tctgtcatct tcagatgtgg cagaataaca ccgtccacga
     5641 cacgaagcgc cacgttccca gccagcaaaa tctcttccat cgtctttccc agctttttct
     5701 ccaggcggta gacatccatc gggtcgagta caatcgaatc tcccatccca tgacagcagg
     5761 cggagcatcc gcggcagtcg tcgcagccca cttttacaag atcattagct ccgtatcgtt
     5821 ttccatctga aattttatct atctctacat ttcgttccat tttttcttcc tatcttttct
     5881 cttaatcctt gcttttggtc tcttctctcg atctttgctt ttaatccttg cttttgatct
     5941 ttgcttttga tcttcgcttt tgatctttgc tttcgatcct tgctttcggt cttttctccc
     6001 gatctttatt tcttcctcac atttctactc ccacaacatg cagtacctcg tcctttacct
     6061 ggaatttgat ttccattccc gcaaaggaaa gtccgtagac gcgttcttcg ctgttctgat
     6121 acgatggacg cggatcct
//
LOCUS       DAOMUF010000126         6127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3470_length_6127_cov_3.371212, whole genome shotgun sequence.
ACCESSION   DAOMUF010000126 DAOMUF010000000
VERSION     DAOMUF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3470_length_6127_cov_3.371212"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLN77_07720"
     CDS             complement(<1..248)
                     /locus_tag="PLN77_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6171 family protein"
                     /protein_id="HRM44195.1"
                     /translation="MDGNTRICKKCLLREMDEAGFFQNMYDYIARIPADDKTPEEEYE
                     RRLSICKECEKLLSGMCRMCGCYVEMRAAIALRDCPGK"
     gene            complement(310..1812)
                     /locus_tag="PLN77_07725"
     CDS             complement(310..1812)
                     /locus_tag="PLN77_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HRM44196.1"
                     /translation="MAKLVIDANRKLSKINKEIQGQFSEHLGRCIYEGLYVGENSDIP
                     NVNGMRTDVVEALKNIKVPVLRWPGGCFADEYHWKDGIGPKESRKKMINTNWGGVVED
                     NSFGTHEFFELCRQIGCETYINGNMGSGTVQEMSEWVEYMTFDGVSPMADLRAENGHE
                     KPWTVDFFGIGNENWGCGGNMNPEFYGNMYRRYQTFVRDYDGNKKIRKIACGANSDDY
                     EWTQEVMKACFRRISPQQHGMMDGLSLHYYTVPETWDHKGSATEFAEKDWYKTMKKTM
                     YMEELIRRHSAIMDQYDPDKKVGMIVDEWGTWYDVEPGTNPGFLYQQNTMRDALVAGI
                     NLNLFNKHSDRVKMANIAQMVNVLQSMILTEGEKLVKTPTYHVFDLYQVHQENDLLAS
                     SLETEQVGLEDEYMVPNLTESVSVDADGVLHITMTNVDLEKAYPVEAVLLGKEAGEIK
                     AEIVTGHMQDKNTFEEPETVGVQGFDGVQATKEGISFTIPACSVLHIAVK"
     gene            complement(2013..2086)
                     /locus_tag="PLN77_07730"
     tRNA            complement(2013..2086)
                     /locus_tag="PLN77_07730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2050..2052),aa:Arg,seq:acg)
     gene            complement(2240..2755)
                     /locus_tag="PLN77_07735"
     CDS             complement(2240..2755)
                     /locus_tag="PLN77_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HRM44197.1"
                     /translation="MSGKERREEILQRITNSKTPVSGAALAKSCEVSRQVIVQDIALI
                     RAAGYDVIATNRGYICSSPVREKRVFEVNHTDEQMQDELNTIVDFGGVVLDVIVRHEV
                     YGELRAELNISSRNKVALFMEEIRQGKSRPLKNITSGEHFHTVSADSAQTLDLIEEEL
                     RKKDYLVRTVK"
     gene            2976..3761
                     /gene="sigG"
                     /locus_tag="PLN77_07740"
     CDS             2976..3761
                     /gene="sigG"
                     /locus_tag="PLN77_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HRM44198.1"
                     /translation="MAINKVELCGVNTSRLPVLNAEEKETLLKKAREGDAAARERYIQ
                     GNLRLVLSVIKRFSNSSENADDLFQIGCIGLIKAIDNFDTTLNVRFSTYAVPMIIGEI
                     RRYLRDNNAIRVSRSLRDTAYRAICARELYLKQNQKEPSIEEIAAEIGIGKEDIVYAL
                     DAIQSPMSLYEPVYTEGGDTLYIMDQLSDKKNKEENWVQNLALQDAVARLNERERQII
                     RMRFYEGQTQMEVAREIGISQAQVSRLEKSALRSMRNYLSDER"
     gene            complement(3772..4575)
                     /locus_tag="PLN77_07745"
     CDS             complement(3772..4575)
                     /locus_tag="PLN77_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM44199.1"
                     /translation="MLVSEMNGIRPVDAGRQDCHDLHSWGPGVRQCYIIHYIISGKGT
                     FTCGKKTYTLTAGQSFLICPEQVVQYAPDENEPWEYVWVDFVGEQCRQILARSVLNPQ
                     QPAAPPLRQERLLPYFERLCQMELYRRNSQEAVGVLLALLGVYQDLAEPQREERKKED
                     PRIMEVMTLIAAGYHRSEFCAETIARKMAVSRTTLNRLFQKKIGRSPGQYLLEYRLQQ
                     SEKLLQMGMTVKQTALSCGFEDPFYYSRVFRKHYGMPPSDYRLQFAEIQ"
     gene            4706..5836
                     /locus_tag="PLN77_07750"
     CDS             4706..5836
                     /locus_tag="PLN77_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HRM44200.1"
                     /translation="MIKLTPPMGWNSWNTFGENINEKMIFETADVMAESGLRDKGYEY
                     LVIDDCWSLRERDENGRLVPDPGKFPHGMKAVADYVHSKGLKFGMYSCAGNMTCAGYP
                     GSYEHEFVDAETFASWDVDFLKYDYCYHSPILHGKYLYRRMGLALENCGRDILFSACS
                     WGADETHEWIKETGASMWRSTGDIFDTWDSVKDLVAQQEKLHPYNGVGCFNDMDMLIV
                     GMHGKGNVGLAGCSDVQYQTHYALWAFLGSPLMIGCDIREMSDETRRILMNDEMIRIN
                     QDPLCRQPYKVKNHAWNDKVLIYVKALADGDLAIGMFNLSDEKSRAAMNLDEIGLPFS
                     TGRTLELTEVWTGENLCANNASLVTELEPFASKVYRAKVVKL"
     gene            5836..6027
                     /locus_tag="PLN77_07755"
     CDS             5836..6027
                     /locus_tag="PLN77_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44201.1"
                     /translation="MARCMQCGKELTHNEIGAHRKFINRGAEQFFCKQCLAKHLGVTP
                     ELIDEKIEQFKRQGCTLFV"
BASE COUNT         1462 a         1707 c         1340 g         1618 t
ORIGIN      
        1 tttttccccg ggcagtcccg cagtgcaatc gctgcccgca tctccacgta gcagccgcac
       61 atgcggcaca tgccggacag cagcttttca cactccttac agatggaaag ccgccgctcg
      121 tattcctcct ccggcgtttt atcgtccgcc ggaattctcg caatataatc atacatattc
      181 tgaaaaaagc ctgcctcgtc catctcacga agcaggcatt ttttacagat cctggtattt
      241 ccgtccatca aactcctccg gaaccgaatc ttttttcatc ccagatatct tctggttttt
      301 atggttttcc tattttacgg cgatatgaag cacactgcac gccggaatgg taaaggaaat
      361 tccctctttt gttgcctgca caccgtcaaa gccctgaacg ccaaccgtct ccggctcttc
      421 aaaggtgttt ttatcctgca tatggccggt tacgatctcg gctttgatct cgccggcttc
      481 ttttccgaga agtaccgcct ctaccggata tgctttctcc agatccacat tcgtcatcgt
      541 gatatgaagt acaccgtctg catctaccga aacagattcc gtcagattcg gaaccatgta
      601 ttcgtcttcc agaccaacct gctccgtttc cagagagctt gccagaagat cattttcctg
      661 atgtacctga tacagatcga aaacatgata cgtcggagtt ttgaccagct tttccccctc
      721 ggtcaggatc atcgactgca gaacattgac catctgtgcg atatttgcca tcttgacacg
      781 gtcgctgtgt ttgttgaaga gattcaggtt gatccctgcg accagtgcgt cacgcatggt
      841 attctgctga tacagaaatc ccggatttgt tccaggctct acatcatacc aggtacccca
      901 ctcatccacg atcatgccga cctttttgtc cgggtcatac tgatccataa tggcgctgtg
      961 gcgacggatc agctcttcca tatacatcgt ctttttcatg gtcttatacc agtctttttc
     1021 cgcaaactcc gttgcacttc ccttgtgatc ccaggtttcc ggcaccgtat agtaatgaag
     1081 cgacaggcca tccatcatgc catgctgctg tggggagatt ctgcggaagc atgctttcat
     1141 cacttcctgc gtccactcat aatcatccga attggcgccg caggcaatct tccggatctt
     1201 tttgtttcca tcgtaatcac gcacaaaggt ctgatatctg cggtacatat ttccgtagaa
     1261 ttccggattc atatttccgc cgcagcccca gttctcattg ccgatgccga agaaatcgac
     1321 ggtccacggc ttttcgtggc cattttccgc acgaaggtct gccatcggag ataccccgtc
     1381 aaatgtcata tattcaaccc actccgacat ctcctgtaca gtaccgcttc ccatatttcc
     1441 gttgatatag gtttcacatc cgatctgacg gcagagttca aaaaactcgt gggtaccgaa
     1501 gctgttgtcc tcgacaacac cgccccagtt ggtgttgatc atttttttgc ggctttcttt
     1561 cggtccgata ccgtctttcc agtgatattc atctgcaaaa catccgcccg gccagcgcaa
     1621 taccggaact ttgatatttt tcagtgcttc aacaacatcg gtacgcattc cgttcacgtt
     1681 cggaatatcg gaattttctc ctacatagag tccttcataa atacatcttc ccagatgctc
     1741 cgaaaactgt ccctggattt ccttattgat ttttgaaagc tttctgttcg catcaattac
     1801 taacttagcc ataacttgtc ccctttcgag atcttaaatt tggctttatt ataggagatg
     1861 tatctcactt gtgtcaatga gcgtacaagt tatttcccca tgattttttc aaaaagcgcg
     1921 gccgtctggc tgctgaccaa aaggccgctt gttttatttt tcattctgca tacgaaaaaa
     1981 ccgccctggt tttcaccagg acggttaccg tccgtgcgtt agaggattcg aacccccgac
     2041 cttttggtcc gtagccaaac gctctatcca gctgagctaa acgcacattt ctttgtcgct
     2101 ttagaactct ttgtttatca ctcgttcccg ctgacatcat gtatattaca ctatcctaag
     2161 agaaaagtca acaccttttt gaaaaaattt taaaattttt tcgaataaca gttttttgtg
     2221 aaaataaggg ctttttcccc tatttcacag tccgcaccag gtagtctttc tttcgcagtt
     2281 cctcttcaat cagatccagg gtctgcgcag agtctgccga tacggtatga aaatgttccc
     2341 ctgatgtgat attcttcagc ggccgtgatt ttccctgccg gatttcttcc ataaacagtg
     2401 ccaccttatt tcgtgaactg atattaagtt ccgcccgcag ttctccgtaa acctcgtgac
     2461 gcacaatcac atccagcaca acgccgccga aatccacaat tgtattcagc tcgtcctgca
     2521 tctgctcatc cgtgtgattc acctcaaaga cccgcttctc tctcaccggg ctgctgcaga
     2581 tgtagccgcg gtttgtcgca atcacatcgt atccggcagc gcggatcagg gcaatgtcct
     2641 ggacaatcac ctgacggctg acttcacagc tcttcgccag cgctgcaccg gagaccggcg
     2701 tcttactgtt tgttattctt tgaagaatct cttctcttcg ttcctttcca ctcatcataa
     2761 aacaagacct cgcatttcgt acacttccct tatcttatca cactttccgc cgtttctaca
     2821 atagcaggaa atcattttcc aaaagctttg tcaaggacta aaactttatg aaattttccg
     2881 ggctctcgta tattcgtccc gccaactgcc aatcatgaac gtataacagt aacgaacggt
     2941 aaccatacgt tccatgtttc aggaggatgc ataccatggc gatcaataaa gttgaactgt
     3001 gcggagtcaa tacatcacgc cttcccgtac tgaatgccga agaaaaggag actctgctga
     3061 aaaaggcccg cgaaggagat gctgctgccc gcgaacgcta cattcagggc aatctgcgcc
     3121 ttgtcttaag cgtgatcaag cgcttctcca acagcagtga aaacgccgac gatctctttc
     3181 agatcggctg tatcggcctg attaaggcga ttgataactt cgacacgacc ttaaacgtcc
     3241 gtttctccac ctatgccgtg ccgatgatca tcggtgaaat ccggcggtat ctgcgtgaca
     3301 acaatgcgat ccgcgtcagc cgttccctca gggatactgc ctaccgcgcc atctgcgccc
     3361 gcgaacttta tctcaagcag aaccagaaag agccttcgat cgaggaaatc gccgctgaaa
     3421 tcggcattgg aaaagaggat atcgtctatg ccctcgacgc aatccaaagc ccgatgagtc
     3481 tgtatgagcc cgtctacacg gaaggcggcg ataccctgta tatcatggat cagttaagtg
     3541 ataagaaaaa taaagaagaa aactgggtgc agaacctcgc cctgcaggac gcggtcgccc
     3601 gtctcaatga gcgcgaacgc cagatcatcc gcatgcgttt ctatgagggg cagacccaga
     3661 tggaggttgc ccgggaaatc ggcatctcgc aggcgcaggt cagccggctg gaaaagtccg
     3721 ccctgcgctc tatgcggaat tatctctccg acgagcggta acgcccgccg ttcactgaat
     3781 ttctgcaaac tgcagccggt aatccgacgg cggcattcca tagtgtttcc ggaacacacg
     3841 ggaatagtaa aagggatctt caaatccgca cgacagcgcc gtctgtttga ccgtcatccc
     3901 catctgcagc agcttttctg actgctgcag ccgatattcc agcagatact gtcctggtga
     3961 ccggccgatt ttcttctgaa acagacggtt cagcgttgtg cggctgaccg ccattttccg
     4021 cgcaattgtc tccgcgcaga actcactcct gtgataccct gcggcaatca gagtcatcac
     4081 ttccatgatc cgcggatctt ccttctttct ctcctcccgc tgcggctccg ccagatcctg
     4141 gtacacgccc agaagcgcaa gcagcacacc gacggcctcc tggctgttcc ggcgatagag
     4201 ttccatctgg cagagacgct caaaatacgg cagaagcctt tcctgacgga gaggcggcgc
     4261 cgccggctgc tgtgggttca gtaccgaccg tgccagaatc tgccggcatt gttccccgac
     4321 aaaatcaacc cacacatatt cccacggctc gttctcatcc ggcgcgtact gcaccacctg
     4381 ctctggacag attaaaaagc tctgtccggc ggtaagtgtg tacgtctttt tcccgcaggt
     4441 aaacgttccc tttccgctta taatataatg tataatatag cactgccgta cacccggccc
     4501 ccaggaatgc agatcatggc agtcctgccg cccggcatcg acaggccgga tcccgttcat
     4561 ttctgataca agcatattct ttccttttcc cgtattttat tctttatgaa gcaaaaatcc
     4621 atgcttttgt cttaaaattc catggttttg ctctggaaat ccatctatac tcaaagtaca
     4681 tcaaaactta cgggaggtca atactatgat caaacttaca ccgccgatgg gctggaattc
     4741 ctggaacacc ttcggagaaa atatcaatga aaaaatgatt tttgaaactg ctgatgtcat
     4801 ggccgagagc ggcctgcgcg ataaaggata cgaatatctt gtcatcgacg actgctggtc
     4861 tttacgtgaa cgcgatgaaa acggccgtct tgtgccggat ccgggaaaat ttccgcacgg
     4921 tatgaaagcc gttgccgatt acgttcattc caaagggctg aaattcggta tgtattcctg
     4981 cgccggaaac atgacgtgtg ccggctaccc tggcagctat gagcacgaat ttgtcgatgc
     5041 ggaaactttc gcatcctggg atgttgattt cttaaaatac gactactgct accacagccc
     5101 gatcctgcat ggaaaatatc tgtaccgccg catgggactg gcactcgaga actgcggccg
     5161 cgacattctc ttctctgcct gctcctgggg tgccgatgaa acgcatgagt ggatcaaaga
     5221 aaccggcgct tccatgtggc gttccaccgg cgacatcttt gatacctggg attctgtcaa
     5281 agatctcgtg gcacagcagg aaaaactgca tccgtacaac ggcgtcggct gcttcaacga
     5341 tatggacatg ctgatcgtcg gcatgcatgg aaaaggaaat gttggactgg ccggctgcag
     5401 cgatgtccag tatcagacac actatgcact gtgggcattc cttggctccc cgctgatgat
     5461 cggctgcgac atccgcgaga tgagcgacga aacccgccgc attctcatga atgacgaaat
     5521 gatccgcatc aaccaggatc cgctctgccg tcagccgtat aaagtgaaaa accatgcatg
     5581 gaatgacaag gttctgatct atgtgaaggc actcgcagac ggagatctcg ccatcggtat
     5641 gttcaatctc agtgatgaaa aatcacgtgc agccatgaat ctggatgaga tcggccttcc
     5701 gttctccact ggccgtactc tggaacttac tgaggtctgg accggcgaga acctgtgtgc
     5761 aaacaatgca tcccttgtca cagagctcga gccgtttgca tcaaaagtat accgtgcaaa
     5821 ggtcgtaaaa ctctgatggc acgctgtatg cagtgcggca aagaactcac gcataacgag
     5881 atcggcgccc accgtaaatt tatcaaccgc ggcgcagaac agtttttctg caagcagtgt
     5941 cttgctaagc atctcggtgt aacgccggag ctcattgacg aaaaaatcga acagttcaag
     6001 cggcaggggt gtacactgtt tgtatagacc tacactgtct gaaatgcaca cagccggatt
     6061 tccgccaaat agaaatccgg ctctattttc atgctgcata aaaaatcatt tcttctttat
     6121 ttctttc
//
LOCUS       DAOMUF010000127         6107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3487_length_6107_cov_4.574686, whole genome shotgun sequence.
ACCESSION   DAOMUF010000127 DAOMUF010000000
VERSION     DAOMUF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3487_length_6107_cov_4.574686"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..333
                     /locus_tag="PLN77_07760"
     CDS             22..333
                     /locus_tag="PLN77_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44202.1"
                     /translation="MESVINKLAEIETAATKIMQAAENQKKLQDEQQEERIAKFDADL
                     EAETAKRIEEITTKLHSTTDADQKKQRDSAAELLSSLDAYYEQNHGTLSTQIYENIIR
                     K"
     gene            338..1378
                     /locus_tag="PLN77_07765"
     CDS             338..1378
                     /locus_tag="PLN77_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRM44203.1"
                     /translation="MSGLLSYSGLTTKIRAMQSHLLNDQNFREIVELESVPQAVSYLK
                     QHKGYEDLFEGYNEADLHRGQIEKMLRLTVYRDFSKLYRFANVEQRKFLALYFKRYEV
                     SVIKECLNTVFDHRDVVLNLSIFDEFFNKHSQLDINLLASSRTIEELIANLKGTEYYA
                     PLSQLADIERPTLFDYEMTLDLYYFAQMWKDRSKVVTKRDLEELTAAWGSKFDMLNLQ
                     WIYRSKRYYHMTSAQIYALLIPVHHRLKKDVVKALVEAENMSAFSQLLEQTYYAKRYD
                     SFKVDTLESMYAAIMKHILSSESRQDPYSVATIYSYLYHKEHEVNRLIIALECVRYRI
                     SADEAMQHIAKT"
     gene            1399..3336
                     /locus_tag="PLN77_07770"
     CDS             1399..3336
                     /locus_tag="PLN77_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRM44204.1"
                     /translation="MIVKMKFLSITGPKADIDRVVNTYLSKYEIHLENALTELSTMQN
                     ITPYIQINPYKETLKQAEDFTALLPEDKRTTTKRLSLEDAINCVHGLDHTLGQLNQER
                     TELEAKKKDLMEKAQKIEPFQDLHCNLSSILDFHFIKFRFGKITKEYYNKFESYCYEN
                     SDTVFYRCHSDEQYVWGIYFCPRDQVTKVDAVFSSLHFERIYVPDEYSGTPEDAYAAL
                     QAEIKKTDDALSECSRKLAKCLSDSAEDLVAAKEQLEQLSGNFDVRRLAACTKSKKET
                     FYILCGWMSEKDAASFQKDIENDTNLFCFIEDDANNVLRQPPTKLKNPKIFRPFEMYV
                     KMYGLPNYNEIDPTIIVGLTYAFIFGIMFADVGQGLCLLIGGALLYHYKKMDLAAIMS
                     TCAVFSVIFGFLFGSFFGFENVIPALWLHPKEAMTTLPFIGSMNTVFVAAVAFGMFMI
                     LTGMVLHMINAARNHKLGELLFSQNSLAGFIFYGALAVTLVLLFSGHKAPAVGVMVVM
                     FGIPLLAIFLKEPLERMVEKKGNAFPETGKGMFFVQAFFELFDVLLSYFSNTISFVRV
                     GAYAVSHAAMMEVVLMLGGATGGGSISWPVIVLGNIFVACLEALLVAIQVLRLEYYEM
                     FSRFYEGDGKPFVPFLKKGEK"
     gene            3464..3898
                     /locus_tag="PLN77_07775"
     CDS             3464..3898
                     /locus_tag="PLN77_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRM44205.1"
                     /translation="MNSAFVQVLTAIAMVLTIVVPMTYYFKGEHNKNRYKKSLAANVS
                     GFFGLLLIATVVSFAGATTASAATEAGAGLATGLGYIGAALVTGLSGIGSGIAVASSA
                     SAALGAISEDGSIFGKSMIFVAMAEGIALYGLIISFMILGKL"
     gene            3949..4257
                     /locus_tag="PLN77_07780"
     CDS             3949..4257
                     /locus_tag="PLN77_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRM44206.1"
                     /translation="MKMFLISDNVDTQTGMRLAGVEGVVVHEREELYDTLQKTLADKE
                     IGIILLTEKFGKEFPDILEDVRLNHKLPLLIEIPDRHGTGRAPDFITSFVNESIGLKL
                     "
     gene            4281..4868
                     /locus_tag="PLN77_07785"
     CDS             4281..4868
                     /locus_tag="PLN77_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HRM44207.1"
                     /translation="MTTEEKLQNFYNHSLDSANREAERVIGDHQKALDKIFEEHKATA
                     RRQAEEEVAAETEKAKRDVNKTLSADQLHIRRKLSRKNLELKEKLFKEVREKLTAYKK
                     DPSYEEYLERKIREAVTFAGNDKLTLYLDPSDEAHKASLEQKLSVTLTISAMPFLGGV
                     RAVIPEKNILIDNSFETLLNEERENFIFHGGVEHE"
     gene            4861..>6107
                     /locus_tag="PLN77_07790"
     CDS             4861..>6107
                     /locus_tag="PLN77_07790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRM44208.1"
                     /translation="MNKTGVIYGINGPVVYLKGNTGFKMSEMVYVGKERLVGEVIALT
                     KDTTTVQVFEETTGLKPGETVTASGDALSVTLGPGILNNIFDGIERPLSEIAKQSGKY
                     ISRGLTVDSLDTEKKWDVHVTVSEGEELMGGAIIAETQETRSIVHKSMVPPDVNGTVI
                     WAAKDGKYTILDPIVKLKLEDGTEKEITLAQKWPIRVPRPTLKRYPASVPLITGQRIL
                     DTMFPIAKGGTAAIPGGFGTGKTMTQHQIAKWSDADIIIYIGCGERGNEMTQVLEEFQ
                     ELIDPKSGNPLMDRTTLIANTSNMPVAAREASLYSGLTLAEYYRDMGYDVAIMADSTS
                     RWAEALRELSGRLEEMPAEEGFPAYLASRLSAFYERAGMMQTLCGAEGSVSIIGAVSP
                     QGGDFSEPVTMNTKRFVRCFWGL"
BASE COUNT         1711 a         1446 c         1514 g         1436 t
ORIGIN      
        1 ataaggaggg aaagcgatgc tatggaatct gtaattaaca aacttgcaga gatcgaaacg
       61 gctgctacta agattatgca ggctgccgag aatcagaaga agctccagga tgagcagcag
      121 gaagagcgga ttgcaaaatt cgatgcagat ctggaagcgg aaacggcaaa gcggattgaa
      181 gagatcacaa caaaacttca ttctactaca gatgcggatc agaaaaagca gcgggactct
      241 gccgccgaac ttctatcttc actggacgct tattacgaac agaatcacgg aacactgtcc
      301 acgcagattt acgaaaacat tataaggaag tgaaacgatg agcggtctat tatcctacag
      361 cggccttacc acaaagataa gagccatgca gagccatctt ttaaacgacc agaattttcg
      421 ggaaatcgtg gaactggaga gtgtaccgca ggcagtttct tacctcaagc agcataaagg
      481 ctatgaggat ctgtttgagg gctacaacga agctgacctt caccgaggtc agatcgagaa
      541 aatgctgcgc ctgacggtgt accgggattt ttccaagctg taccgttttg caaatgtaga
      601 gcagagaaag tttcttgcac tctacttcaa acgatacgaa gtgtccgtta tcaaagaatg
      661 cctgaacacg gtatttgatc accgtgatgt ggtattgaac ctgtccatat tcgacgagtt
      721 ctttaacaag cattcccagc tggacatcaa tcttctggcg tcctccagga cgatcgaaga
      781 actgattgcg aacctgaaag ggacagaata ttatgccccg ttgtcgcagc tcgcggatat
      841 cgagcgtccg accctgttcg actatgagat gaccctggat ctgtattact ttgcacagat
      901 gtggaaagac agatcaaaag tagtgacaaa gcgggatctg gaagagctga ccgctgcatg
      961 gggaagcaag tttgacatgc tgaaccttca gtggatctac cgttccaaac gctattatca
     1021 catgaccagc gcacaaatct acgccctgct gattccggtt catcaccggc tgaaaaagga
     1081 tgtggtaaag gcactggtag aagccgagaa tatgagtgca ttttcccagc tgctggaaca
     1141 aacctattat gcgaaacggt atgattcctt caaggtcgat acgctggaat ccatgtatgc
     1201 agccattatg aagcacatac tttccagtga atccagacag gatccttatt ccgttgccac
     1261 catatactca tacctctatc acaaagagca cgaggtaaac cggcttatta ttgcactcga
     1321 gtgcgtacgt taccgtatca gcgctgacga ggcaatgcag catatcgcaa aaacttaata
     1381 cacggggagg tgtactcatt gattgtcaag atgaaatttt tgagcatcac cgggccgaaa
     1441 gccgacattg accgtgtggt caacacttat ctctcaaaat atgagataca tctggaaaac
     1501 gctctgacgg agctgtctac gatgcagaat atcacaccgt atatccagat caacccatac
     1561 aaagagacct taaaacaggc ggaagatttt acagcacttc tgccggaaga caagcgtacc
     1621 accacgaaaa gactttccct ggaagatgcc atcaactgtg tccatggttt ggatcacacc
     1681 cttggccagt tgaaccagga gcgtacagaa ctggaagcaa aaaagaaaga cttgatggaa
     1741 aaagcacaga aaatcgagcc gttccaggat ctgcactgca atctttcctc cattctggat
     1801 ttccacttta tcaaattcag attcggaaag atcacaaaag agtattacaa caagtttgag
     1861 agctactgct atgagaacag cgatacggta ttctaccgct gccattccga tgagcagtat
     1921 gtatggggca tttacttctg cccaagagat caggtcacga aagtggacgc cgtattctca
     1981 tccctgcact tcgaaagaat ctacgttccg gacgagtaca gcggaacgcc ggaggatgct
     2041 tacgcagcac ttcaggcaga aatcaaaaag acagacgatg cactcagcga gtgctcacgg
     2101 aaactggcaa aatgcctttc ggattccgca gaggatctgg ttgcagcaaa agagcagtta
     2161 gagcagctct ccggcaactt tgatgtccgt cgcctggcag cctgcacgaa atccaagaag
     2221 gaaaccttct atattctgtg cggctggatg tcggaaaaag atgccgcttc cttccagaag
     2281 gacatcgaaa acgacacaaa tctcttctgc tttatcgagg atgatgcaaa caacgttctg
     2341 cgtcagccgc cgacaaagct gaaaaatccg aaaatcttcc gtccgtttga gatgtacgtg
     2401 aagatgtacg gactgccaaa ttacaatgaa attgacccta cgattatcgt aggactcacc
     2461 tatgccttta ttttcggaat catgtttgcc gatgtaggac agggattgtg tctgctgatt
     2521 ggcggcgcac tgctgtacca ctacaagaag atggatctgg cagcgatcat gagtacctgc
     2581 gcggtattct ctgtgatctt cggattcttg ttcggcagct tcttcggatt tgagaatgtc
     2641 ataccggccc tctggctgca tccgaaggaa gcgatgacaa cgcttccgtt catcggttcg
     2701 atgaacaccg tatttgtcgc agcggttgca tttggtatgt tcatgattct gacaggaatg
     2761 gttctgcata tgatcaatgc agcaagaaac cataagctcg gcgagcttct gttcagccag
     2821 aactccctgg caggcttcat tttctacgga gcccttgccg ttacactcgt tctcctgttc
     2881 agcggccata aagctccggc tgtcggcgtg atggtagtca tgttcggtat tccgcttctt
     2941 gcgatcttct taaaagagcc actggagcgt atggtagaga agaagggcaa tgcatttccg
     3001 gagaccggaa agggaatgtt ctttgtacag gcattcttcg aactcttcga tgtgcttttg
     3061 agctacttct ccaacacaat ttcctttgtc cgtgtcggcg catatgcagt cagccatgcg
     3121 gctatgatgg aggttgtcct gatgctcggc ggagcaacag gcggcggcag catcagctgg
     3181 ccggttattg ttctcggaaa catcttcgtt gcctgccttg aggcactgct ggtagcgatc
     3241 caggtactgc gtctggagta ttacgagatg ttcagccggt tctatgaagg agacggaaaa
     3301 ccgtttgttc cgtttttgaa aaaaggcgag aaataaaaga ggattcagag ccatgcaaaa
     3361 tggaagcgaa tatggcggga agctgtatcg ggaaaaagtg aaaatggctc cggatcgcag
     3421 caaagagtaa atttaatgaa ttaacttcag gaggaaaact attatgaact cagcatttgt
     3481 acaggtactt actgcaatcg caatggtatt aaccatcgtt gttcctatga cttattactt
     3541 caaaggtgag cacaacaaaa atcgttacaa aaaatctctg gcagccaatg taagcggctt
     3601 cttcggcctt ctgctgatcg caaccgttgt ttccttcgca ggcgcaacaa ctgcatctgc
     3661 tgcaacagaa gcaggcgctg gtcttgcaac cggtcttggt tacatcggtg cagctcttgt
     3721 tacaggtctt tccggtattg gttccggtat cgccgttgca tcttccgcaa gcgcagctct
     3781 tggagcaatc agcgaagacg gcagcatctt tggtaaatcc atgatcttcg tagctatggc
     3841 cgaaggtatc gctctgtatg gtctgatcat ctccttcatg attcttggta aactttgatt
     3901 ccaaaaccgg aaggtattga tcttcacctt aagttggagg aatcataaat gaaaatgttc
     3961 ttgatcagtg ataacgtaga cactcagacg ggcatgcgcc tggccggagt ggaaggcgtt
     4021 gtcgttcatg agcgcgagga gctttatgat acactgcaga aaacactggc agacaaagag
     4081 atcggaatta ttctccttac agaaaagttc gggaaggaat tcccggacat tctggaggat
     4141 gtccgcctca accataagct tccgctgctc attgaaattc cggaccgtca cggtaccgga
     4201 agagcgccgg actttatcac ttcctttgta aatgaatcta ttggattaaa actgtaagaa
     4261 tgccctggag gtgacacaca ttgactactg aggaaaaact tcagaatttt tataatcatt
     4321 ccctggacag tgcaaaccgt gaagcagaac gtgtgatcgg cgatcaccag aaagctctgg
     4381 ataagatctt cgaggagcat aaagcaaccg caagacgtca ggcagaagaa gaagttgctg
     4441 ccgagaccga gaaagcaaaa cgtgatgtca ataagacact gtctgcggat cagcttcata
     4501 tcagacggaa gctgtcccgt aaaaatctgg aattaaaaga aaaactgttt aaagaagttc
     4561 gtgaaaaact taccgcttat aaaaaggatc catcctatga ggagtatctg gagcgcaaaa
     4621 tccgcgaagc cgttacattt gccggaaacg acaaactgac cttatatctg gatccgtcag
     4681 acgaagctca taaggcttcc ctggagcaga aactctccgt aacccttacc atttcggcca
     4741 tgccgttcct tggcggcgtg cgcgcggtca ttccggagaa aaatattctg atcgataatt
     4801 ccttcgagac ccttctgaac gaagagcggg agaatttcat ctttcacgga ggtgtagagc
     4861 atgaataaaa ccggagttat ctatggaatc aacggtccgg tcgtatatct gaaaggaaac
     4921 accggattta aaatgtccga gatggtttac gttggtaaag aacgtctggt cggcgaggtt
     4981 attgccctga caaaggatac caccacagta caggttttcg aggaaaccac aggactgaaa
     5041 ccaggcgaaa cagttaccgc atccggagat gccctctctg ttacactggg acctggaatc
     5101 ctgaacaaca tttttgacgg tattgagcgt ccgctgtccg agattgcaaa gcagtccggt
     5161 aaatacatca gccgcggcct taccgtagat tctctggata cagagaaaaa atgggatgtc
     5221 catgtaactg tttcagaagg cgaagagctg atgggcggcg caatcattgc agagacacag
     5281 gagacccgtt ccatcgtaca caaatcgatg gttccgccgg atgtcaacgg cacggttatc
     5341 tgggctgcca aagacggaaa gtacaccatc cttgacccga ttgtaaaact gaaactggaa
     5401 gacggcaccg aaaaagagat cacactggca cagaaatggc cgatccgtgt accgcgtcca
     5461 accctgaaaa gatatccggc atccgttccg ctgatcacag gacagcgtat cctggatacg
     5521 atgttcccga tcgcaaaagg cggcacagca gccatccctg gcggattcgg taccggaaaa
     5581 accatgaccc agcaccagat cgcaaaatgg tctgatgccg atatcatcat ctatatcgga
     5641 tgtggagagc gtggaaacga gatgacacag gttctggagg agttccagga actgatcgac
     5701 ccgaagagcg gcaacccgct gatggaccgt accaccctga tcgccaacac atccaacatg
     5761 cctgttgcag cccgtgaggc atctctgtat tccggactga cactggctga gtattaccgc
     5821 gatatgggct atgatgtagc aatcatggca gactccacat cccgttgggc agaggcactc
     5881 cgtgagctgt ccggccgtct ggaggaaatg cctgccgagg aaggtttccc ggcatacctg
     5941 gcatcccgtc tgtccgcttt ctatgagcgt gccggcatga tgcagaccct ttgcggcgca
     6001 gagggaagcg tatccatcat cggagccgta tctccgcagg gtggtgactt ctccgagcct
     6061 gttaccatga acaccaaacg tttcgtccgc tgcttctggg gactgga
//
LOCUS       DAOMUF010000128         5926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3633_length_5926_cov_4.183103, whole genome shotgun sequence.
ACCESSION   DAOMUF010000128 DAOMUF010000000
VERSION     DAOMUF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_5926_cov_4.183103"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..780
                     /locus_tag="PLN77_07795"
     CDS             316..780
                     /locus_tag="PLN77_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM44209.1"
                     /translation="MTQEKLKQFLDACFDAKRLIEKLPDLPDGMKPRQVHVLDAVNEI
                     QKKQGFCRVSDISSYMNTTLPSITKLVQELEERNLLVKQADEKDKRVINLTLTEEGKE
                     FVELRVTHFHGEWASRLPDLDDAVVDEFVHAITRMQETMPGIKGVKNNGKRK"
     gene            764..2113
                     /locus_tag="PLN77_07800"
     CDS             764..2113
                     /locus_tag="PLN77_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44210.1"
                     /translation="MAKENKKEISFVEGPIFQSLIRFALPVLGALILQAAYGAVDLLV
                     VGWFGDASSISAVGTGSNFMQMVTLIITSLAMGSTVMIGQHIGEGKPDKAGKTVGTSI
                     VLFAIIGIAMTVILELFAGPIANILQVPAESFDKTVLYLRICSGGILIIIAYNVISSV
                     LRGIGNANLPLLFVGIACAANIAGDLFFVGVLKLDVAGAALATVLAQLISVIASMGVL
                     KKGNLPIEFKKEDCRIDHDELKKVLNVGVPIALQETTVQISFLVINSIINGMGLMPSA
                     GYGVAQKLTSFIMLIPSSVMQSVSAFVAQNTGAGKKDRAWKGFLTAIATGCSLGVVIF
                     CIGFFGGSVISRIFTSDPAVIEQSAAYLRGFAPECILTCVLFSSIGYFNGRGISIPVM
                     IQGITSACLIRIPLALFFSHLPNTNLTFVGMSTPITTVYGICFFMICFARLKKKGQM"
     gene            2277..2714
                     /locus_tag="PLN77_07805"
     CDS             2277..2714
                     /locus_tag="PLN77_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRM44211.1"
                     /translation="MDNREIINETLVHLFHEIQKLEEEAIITEDFKDLTNNDMHIIEA
                     VGLEGGNMSSIAAKLDITVGSLTTSMNSLVKKGYTERERSEKDRRIVYIHLTGKGRQA
                     FHHHAKFHEQMTDAVMAELGEEEVQVLGKTLKALTDFFRSYKK"
     gene            complement(2805..3446)
                     /gene="nth"
                     /locus_tag="PLN77_07810"
     CDS             complement(2805..3446)
                     /gene="nth"
                     /locus_tag="PLN77_07810"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRM44212.1"
                     /translation="MTKEELALAVIERLKQEYPDADCTLDYDQAWKLLVSVRLAAQCT
                     DARVNVVVEDLYAKYPDIDALANATPEEIEEIVRPCGLGKSKARDINACMRMLRDEYH
                     SQVPDDFNALLKLPGVGRKSANLIMGDVFKKPAIVTDTHCIRLCNRIGLVHDCKDPKK
                     VEMALWKIVPPEEGSSLCHRFVEHGREVCTARTTPHCERCVLADICEKNGVEK"
     gene            complement(3492..4367)
                     /locus_tag="PLN77_07815"
     CDS             complement(3492..4367)
                     /locus_tag="PLN77_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM44213.1"
                     /translation="MPRKDHLPRQTRQEHITLQPNRSIEFRVTDDFGSFSPPHWHDAL
                     EIIYILEGSSIVTLSDRTKTVLPGQFLLINSGRIHTARCPSTGNRSILMQIPDAFLAN
                     YLPDPSQLWFSIDYDSTNPEVQKNISQFRRLLLSMMQLHDEMPDGYLLTFQRELFTFL
                     DLLYTKFSEKSPLAHHPMSSRTQTRLDTILTYTQENYQRPITLKEAADVVSLQPEYFC
                     RFFRQNMETTYLEYLNSYRLSRIYRDLIATDLPVGTLAEKHGFTNDKLFHRLFRERFH
                     TTPLQLRKAAREKGS"
     gene            4535..5485
                     /locus_tag="PLN77_07820"
     CDS             4535..5485
                     /locus_tag="PLN77_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRM44214.1"
                     /translation="MYQVWSNFLNPGQIAMLGIVVTFLLTFLALKHPFSFLPSDHGRE
                     FAVNGGLSRGKLRGVGLVIVICFLIGSVLFLPLGAEYVIYAILLVCIMLSGYLDDASE
                     TPWSDYKKGAIDLVISIVTVITFVNYNSTTIYFGSMSLTIPKVVYIILGIILIWISIN
                     VTNCSDGVDGLCASLSCVVIFAFSMIFHDVLGSFTVANYLFVAALLAYLYFNTSPSSM
                     LMGDAGSRALGFYIAILAMQSRHPFLYLLMAGVMIVDGGIGLVKVFLLRFLKIAILKN
                     TRTPLHDHVRLKYKWSDSQVVIRFVILQILLAVIAYLIVA"
BASE COUNT         1490 a         1502 c         1539 g         1395 t
ORIGIN      
        1 caatgcattc tcagaggata ttcagcattc tctggcagcc ggtatgaatg cccatgtttc
       61 aaaaccggtt gagatgaagg tactggaaaa gacgatcaga agcataaaat ccggcggggg
      121 gcaccgaaat gcagctcatt gaacagtgac aaatggatat ggaaaagagc tgatgaaaga
      181 atctggcatt gccagaactt tcaatcagct cttttttatc atcagttccc tcttgacatt
      241 ttttcatttc ctgctatact tatcaaagat aattactaaa gataactaaa tttcaagaag
      301 aaaggaccat tttccatgac acaggaaaaa ttaaagcagt tcctggatgc gtgttttgat
      361 gcaaagcgtc tgatcgagaa gctcccggac ctgccggacg gtatgaagcc gcggcaggtg
      421 catgtgctgg atgcggtgaa tgagattcag aaaaagcagg gcttctgccg cgtcagcgac
      481 atcagcagct atatgaacac aacgctgccg agcatcacca agctggtgca ggaactggag
      541 gagagaaatc ttctcgtgaa acaggcggat gaaaaagata agcgcgtgat caacctgaca
      601 ctgaccgaag agggaaaaga gtttgtggaa ctccgcgtga cgcactttca cggggaatgg
      661 gcatcccgcc tgccagatct ggacgacgcg gtggtggacg aatttgtgca tgcaatcaca
      721 cggatgcagg aaacgatgcc gggaataaaa ggagtgaaaa acaatggcaa aagaaaataa
      781 gaaagaaata tcgtttgtag agggaccgat ttttcagtcc ctgatccgct ttgcgctgcc
      841 ggtgctcggc gcgctgattc tgcaggcggc ttacggtgcg gtcgaccttc tcgtggtcgg
      901 ctggttcggc gatgcgtcga gtatttccgc tgtcggaacc ggaagcaact tcatgcagat
      961 ggtaacactt atcatcacca gccttgcgat gggttcgacc gttatgatcg gccagcacat
     1021 cggcgagggc aagccggata aggccggcaa aaccgtggga acctcgattg ttctgttcgc
     1081 catcatcggt atcgccatga cggtgattct ggagctgttc gcaggtccga tcgccaatat
     1141 tttacaggtt ccggcggagt cttttgacaa aaccgtcctc tacctgcgga tctgttccgg
     1201 cggtattctg atcatcatcg cgtacaacgt catcagcagc gtgcttcgcg gaatcggtaa
     1261 tgcaaatctg ccgcttttat tcgtcggcat cgcctgcgca gccaatattg ccggagatct
     1321 gttcttcgtc ggtgtgctga aactggacgt tgcgggagca gcgcttgcga cggttctggc
     1381 gcagctgatt tctgtcatcg cctccatggg tgtgttgaaa aaaggcaatc tgccgattga
     1441 attcaaaaaa gaagactgcc gcatcgacca tgacgagctg aagaaagtcc tgaacgtcgg
     1501 tgtgccgatc gcgctgcagg agacgaccgt acagatttcc ttccttgtga tcaactcgat
     1561 catcaacggc atgggcctga tgccgtccgc cggatacggt gtggcgcaga agctgacctc
     1621 attcatcatg ctgatcccgt cctctgtgat gcagtctgtg tcagcattcg tggcacagaa
     1681 caccggagcc ggaaagaaag accgcgcgtg gaagggcttc ctcaccgcaa tcgcaacggg
     1741 ctgctctctt ggagttgtga ttttctgcat cggtttcttc ggtggaagtg tcatctccag
     1801 aatcttcacg agcgatccgg ccgttatcga gcagagcgcc gcttacctgc gcggtttcgc
     1861 accggaatgt attctgacct gtgtgctgtt cagcagcatc ggatacttca acggccgcgg
     1921 catcagcatc ccggttatga tccagggaat cacctccgcc tgcctgatcc gtattccgct
     1981 ggcgctgttc ttctcacacc tgccgaatac gaacctgacc ttcgtcggca tgtccacccc
     2041 gatcacaacc gtatacggca tctgcttttt catgatctgc tttgcacggt taaagaaaaa
     2101 agggcagatg taattgaatc aaacataaca gaactgcctg ggaaacggca cggagcgatc
     2161 cgccgtttcc caggcagttt ttggtattgt ttgtccggaa cttttttgat acaatacaga
     2221 cagaaagagc ggaaccctgt tcagaaggga cagaacagac ggaaagggga agaggagtgg
     2281 ataacagaga aatcatcaat gagacactgg tccatctgtt tcacgagata caaaaactgg
     2341 aagaagaggc gatcatcacc gaggatttca aggatctgac gaacaacgat atgcacatca
     2401 tagaagccgt cggcctggag ggcggcaata tgtcatcgat cgcggcaaag ctcgacatca
     2461 cggtcggatc gctgacaacg tcgatgaaca gtctggtgaa aaaaggctac accgagcggg
     2521 agcgcagcga gaaggaccgc cggatcgtct acatccacct gaccggaaag ggacggcagg
     2581 cattccatca tcacgcaaaa ttccacgagc agatgacgga tgccgtgatg gcggaactcg
     2641 gagaagagga agtgcaggtg ctcgggaaaa cgctgaaagc gctgacggac ttctttcggt
     2701 cgtataaaaa atagaaacaa aaaggctttt gcagccggga cagaatcaga aaaatctgtc
     2761 cggtatgcaa aagccttttt cgcatggaaa cgcataaaaa aatgctattt ttctacgccg
     2821 ttcttctcac agatatccgc gagcacacac cgttcacagt gcggtgtcgt ccgcgcagta
     2881 cagacctcgc gtccgtgctc cacaaaccgg tggcagagcg agcttccttc ttccggcggc
     2941 acaattttcc agagtgccat ctccaccttc ttcggatcct tgcagtcatg cacgagcccg
     3001 atgcggttgc acagccggat gcagtgcgta tccgtcacaa tcgcaggctt tttgaataca
     3061 tcgcccataa tcaggttcgc acttttccgt cccacgcccg ggagcttcaa aagtgcgtta
     3121 aagtcatccg gcacctggct gtggtactcg tcgcgcagca tgcgcatgca ggcgttgatg
     3181 tcgcgtgcct tgctctttcc aagaccgcac ggccgcacaa tttcctcgat ttcctccggt
     3241 gtcgcgtttg ccagcgcatc gatatcagga tactttgcgt agagatcctc caccacaaca
     3301 ttgacccgcg catcggtgca ctgtgcggcc aggcggacac tgacgagcag cttccacgcc
     3361 tggtcatagt caagcgtaca gtcggcgtca ggatattctt gtttcaaacg ttcaatgacg
     3421 gcaagcgcca gttcttcttt tgtcatcgtg ttaaactccc attctcaaag atattaaaac
     3481 agcgaaagct atcaggatcc tttctcgcgt gcggccttcc gaagctgcag cggcgtcgtg
     3541 tgaaaccgct cgcggaagag gcggtgaaac agcttatcgt ttgtaaagcc atgcttttcc
     3601 gcgagcgtgc cgaccggcag atccgtggcg atcaggtcgc ggtagatgcg ggaaagacgg
     3661 taggaattca agtattccag atacgtcgtt tccatgttct ggcggaagaa ccggcagaaa
     3721 tattccggct gcagcgagac aacatcggca gcctccttca gcgtaatcgg ccgctggtaa
     3781 ttttcctgcg tgtacgtcag aatcgtatcg agccgtgtct gcgtccggga gctcatcggg
     3841 tggtgcgcaa gcgggctctt ctcagaaaat ttcgtgtaga gcagatcgag aaacgtaaaa
     3901 agctcccgct ggaacgtcag cagatagccg tcgggcattt cgtcatgaag ctgcatcatc
     3961 gaaagcagaa gacgccggaa ctggctgata tttttctgca cttccggatt cgtactgtcg
     4021 tagtcaatcg agaaccagag ctgtgacgga tcaggcagat agttcgcaag gaacgcatcc
     4081 ggaatctgca tcagaatcga gcggtttccc gtggacggac agcgcgccgt gtggatacgc
     4141 ccggaattga tcagcagaaa ttgacccgga aggacggtct ttgtgcggtc cgagagcgtg
     4201 acaatggagg aaccctccag aatataaata atttccagcg cgtcgtgcca gtgcggcggc
     4261 gaaaaactgc cgaaatcgtc ggttacacga aattcgatgc tgcggttcgg ctgcagcgtg
     4321 atgtgttcct ggcgtgtctg gcgcggcaga tgatctttgc gtggcataaa aaactccttt
     4381 tttgttcaac ttagtgtatc actatcttat cacgaaaccc ccgaattggg tatagcaatt
     4441 tttgtatgaa gttgttataa taaatgaaca caaagccacg tcacatacgg cttcgtgcaa
     4501 actcaatcta aagaaaagtg aaggaagaaa acgtatgtat caagtatgga gtaacttttt
     4561 aaatcccggc cagatcgcca tgctgggaat cgtggtcacc tttctgctga cctttctggc
     4621 gttaaagcat ccattttcct ttctgccgag cgatcacggc agagaatttg ccgtaaacgg
     4681 cggcctctcc cgcggaaaac tgcgcggcgt cggccttgtg atcgtcatct gtttcctgat
     4741 cggaagcgta ttgttcctgc ctcttggcgc cgagtatgtg atctacgcga tcctgctcgt
     4801 ctgcatcatg ctgagcggct acctcgatga tgcatccgaa accccgtgga gcgattacaa
     4861 aaaaggcgcg attgacctcg tcatctccat tgtgaccgtc atcacctttg tgaattacaa
     4921 ttccaccacg atctacttcg gaagcatgtc ccttacgatt ccgaaagtgg tctacataat
     4981 cctcggcatc atcctgatct ggatttcgat caacgttaca aactgttccg acggtgtcga
     5041 cggactctgc gcgagccttt cctgcgtggt tatcttcgcg ttcagtatga ttttccacga
     5101 tgtccttgga agcttcaccg tagccaatta cctgttcgtg gcagcgcttc tcgcgtacct
     5161 gtatttcaac acctcgccga gcagcatgct gatgggagac gccggttcga gagcacttgg
     5221 cttctacatc gccatccttg ccatgcagtc ccgccatccg ttcttatatc tgctgatggc
     5281 aggcgtgatg atcgtcgacg gaggaatcgg ccttgtcaaa gtatttttac tccgtttcct
     5341 gaaaatcgcg atcctcaaaa atacaagaac accgctgcat gaccatgtcc gcctgaaata
     5401 caaatggtcc gacagtcagg ttgtgatccg ctttgtgatc ctgcagatcc tgctggccgt
     5461 cattgcgtat ctgattgtag cataagaaaa aaaagaagag aaacgggagt gtgtcttttg
     5521 cagacgtctg cggggcatac tcccgttttg catcccagcc ctcgaataca ccaaaaagat
     5581 agaaaaagat aatatcgccg tactttttca cagaatccgc cctgttttcg aaagcaagag
     5641 tgtggtagta taaaattacc aaaaaatgtc agcctgaaac aataattctg taaagattga
     5701 tgaaaagaaa gtgctttcag ctatctgttt caggctcatc caatcaggag gtaatgaaaa
     5761 gatgaaattc acagaaggtt actggcttcc gagtgaaaaa gcaaatgcat cccatgcaat
     5821 gcaggcttat gaagtagagc agattccagg cggtatgcgt atcctggcta ccacacgtcc
     5881 gatcgagcac agaggcatga ccctgaacct gccgaccatc accgtt
//
LOCUS       DAOMUF010000129         5905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3653_length_5905_cov_2.889915, whole genome shotgun sequence.
ACCESSION   DAOMUF010000129 DAOMUF010000000
VERSION     DAOMUF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3653_length_5905_cov_2.889915"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1020
                     /locus_tag="PLN77_07825"
     CDS             151..1020
                     /locus_tag="PLN77_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRM44215.1"
                     /translation="MIKTIRGFMKRLNEDHVGAYAAQSAYFILLSFIPFVLLVVTLVK
                     YTPLTRDDVYSVLINMLPTEFQSYVGGIVNEIFYQSVAYMPITIITTLWSAGKGIAAL
                     TNGLNSIYHVTETRNYIINRLRGMAYTLIFVVAFLTSLVLLVFGTRIQNKLTEHLPMV
                     ARVTSSIMGMRTLITTGVLAVLFLVLYKCIPNRKASFKSQCPGALISSLAWSIFSLAF
                     SMYLDIALAASNMYGSLTMIVFIMIWMYFCMWILLIGAEINAYFEDKLRKLQNAAIEH
                     FMENKETNKEAGK"
     misc_binding    1093..1345
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1511..2557
                     /gene="pheS"
                     /locus_tag="PLN77_07830"
     CDS             1511..2557
                     /gene="pheS"
                     /locus_tag="PLN77_07830"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRM44216.1"
                     /translation="MQEKSVLTGHLSELKQKIENGLEELKSSKELYEFKSEYLEGKKS
                     KISGLMKEMKNIAPEERANYGKSVNELKEWALGRFQKVEEEMKERELQKKYESEKIDL
                     TLPAEETAIGNLHPVTLIRKQLIDIFAGMGFEVYEGREIETDYYNFTALNTPQDHPAR
                     DMQDTFYLSPEFLLRTQTSAGQIHVMENKKPPIKILSPGRVFRSDDDATHSPMFHQME
                     GLVVDKNITLCDLKGMLDVLVQKIYGEGTTTRLRPSYFPFTEPSVEVDCSCFACGGKG
                     CSLCKGTGWIEVLGAGIVNKRVLENCGIDSEEYSGFAFGIGLERIAMLKYGINNIKLL
                     FGSDLRVLNQINEK"
     gene            2589..5033
                     /gene="pheT"
                     /locus_tag="PLN77_07835"
     CDS             2589..5033
                     /gene="pheT"
                     /locus_tag="PLN77_07835"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRM44217.1"
                     /translation="MLVPLSWLKEYVDIDVTPEELEKKLFDGGFEVEERYQVGKDISN
                     VVVGLVEGCEPIPETHLHVCQVNAGEHGAMQICCGADNVHTGGKFPVALPGASVYATA
                     KDHKTIEGVMTIKKGKLRGYESCGMLCSGVELGLTEDLYPGAGYNGLLVLPEDAELGA
                     DVKELTGLDDWIFDISLTANRPDCQSILGIAREVSAMLEKPLKMPATDYTETDVEKEG
                     FSVRVEAPELCPRYSAHYVYDVKIGESPAWMKRRLALVGMSAISNVVDITNYVLKEIG
                     QPMHAFDCSYLEGNEICVRRAKENEKIVTLDEQEYTMNENNLVICDGVKPVALAGVMG
                     GLNSEIRDTTEAVMFESAKFARDNVRKTARALGKATDASARYEKGVDEYSTVLGMKRA
                     LHLMEELGCGKVSRTHFDVNTGNSIDPTPMTVSVSKVNGVLGIEVPEAEILRIMKNLN
                     FAPEINGDELTIQVPAYREDMLPEGENDVERYPDVAEEVIRMYGYDHVTDTFLSAAQV
                     TMGGYNEEQKGELALKNTLCTMGIYECMHYSFFSPADLDLLKLPADAKERNAIEIINP
                     INQDLSLMRTTLAASMLNAISRNEKQGTLEGRLFEVASIYIPESLPLTSYPDERKVLC
                     VGTFGNNESFFTMKGIANTIAESMDIEFTYEPVQKSFLHPYQAVKVLCEGTEIGYFGK
                     AAYDIQNELSMRTSAYIMELDLKELSRWYGKKRTFQPISKFQEEKRDLAFVMDKNISC
                     GDVENCIREANKYVKEIRLFDVYEGGQIPEGKKSMAFTVTFAPKEEAFDFEKVQKFVE
                     KICKKLQNEFNIELRG"
     gene            5039..5686
                     /locus_tag="PLN77_07840"
     CDS             5039..5686
                     /locus_tag="PLN77_07840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM44218.1"
                     /translation="MKLYIMRHGETAWNVEGRLQGQTDTELNENGVRLAKVTAEGLKN
                     IPFDLGISSPLRRAKHTAELVLAGRNVPLTTDDRLMELSFGSWEGLGCRANNFEIPSE
                     HFDDFYRDPFHFRPAGDGETIQEVCDRAEAFYHELTAKPELQDKTILIATHGCCMRAL
                     LRNVYEDKNDFWHGGVPYNCAVSIIEVQNGVSTLLADNKIYYDPSDCVNFYKEVK"
     gene            complement(5668..>5905)
                     /locus_tag="PLN77_07845"
     CDS             complement(5668..>5905)
                     /locus_tag="PLN77_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44219.1"
                     /translation="SVEDTDFSPPATAVMFLLAIHPTASETGDFLSTGVKIRYFYSIT
                     LSIRRQTRFKEAGTKGNTFLLCLPLNIIFYFTSL"
BASE COUNT         1655 a         1364 c         1556 g         1330 t
ORIGIN      
        1 acgcggttac gcatggtatt accggaaatg gaattaaatc ccttgcgaag gaaagaaaag
       61 tatgatatgc tttgtaggct atcctgcaat acaagatgtg tgcaataatg ggatagattc
      121 acgaaaacaa agaaaaggcg gttttgccag atgataaaaa caatccgcgg ttttatgaag
      181 cgcctgaatg aggaccatgt cggtgcttat gcagcgcaga gcgcgtattt cattcttctt
      241 tcctttattc cgtttgttct tctggttgtt acgcttgtaa aatatacgcc gctgacacgc
      301 gacgatgttt acagcgttct gatcaatatg ctgcccacgg agttccagag ctacgtgggc
      361 ggtattgtaa atgagatctt ttatcagtcc gttgcttata tgccgattac gatcatcaca
      421 acgctgtggt cggcgggaaa gggcattgcg gcgctgacaa acgggctgaa cagcatttat
      481 cacgtaacag agacacgtaa ttacatcatc aaccggctgc ggggcatggc atataccttg
      541 atatttgtcg ttgcattcct caccagtctg gtgcttctgg tcttcggaac ccgcattcag
      601 aataagctga cggaacatct gccgatggtg gcgcgcgtga catcctctat catgggcatg
      661 cgtaccctga ttacgacagg cgtgctggcg gttctgttcc tggtgctgta taaatgcatt
      721 ccgaaccgga aggcgagctt taagagccag tgcccgggcg cactgatctc atcccttgcc
      781 tggtctattt tttcccttgc attttccatg tatctcgaca tcgcgcttgc agcgtccaat
      841 atgtacggca gtctgacgat gatcgtgttt atcatgatct ggatgtattt ctgcatgtgg
      901 atccttctga tcggcgcgga gatcaacgcg tattttgagg ataaactgcg gaaactgcag
      961 aacgcggcga tcgagcattt tatggaaaat aaagagacga ataaagaagc gggaaaataa
     1021 tggcttgaca tccgggggtc aagacggtac aataaacatt gtttatgaaa gttttataaa
     1081 taatctaaca ggaaaaatgc tgtgaagatg ttcagagaaa tattttttcc gccagagaga
     1141 gggagtcatc ggctgcaagc tccttcaggt tcaggaatat ttcaaatttc gcattggagc
     1201 agatgtccgg aacgcatagc aggatactgg aaatgcaagt agggacagac gtttggtgta
     1261 cgttacagca ccggttatga gtgtctgcag gaagtatctg cagaaatcag ggtggtaccg
     1321 cgaaatttca gatctcgtcc ctttatgggg cggggtcttt ttttcgtgag caacgagcca
     1381 ggcagccatg cttgcatgga tatctggcga ctgccacgaa aatcagatgc tccgctgttt
     1441 gcagcggggt atctgattgt ttttcacatt taccatcaaa caagcaagaa aaagaaagta
     1501 ggagagtcaa atgcaggaaa aaagtgtatt aaccggccat ctgtctgaat taaaacagaa
     1561 gattgaaaat ggactggaag agctgaaaag ctccaaagaa ctgtatgaat ttaagagcga
     1621 gtatctggaa ggaaagaaaa gtaagatcag cgggctgatg aaagaaatga aaaacattgc
     1681 cccggaagag cgcgcaaact acggtaaatc cgtcaatgag ttaaaagagt gggcgcttgg
     1741 ccgcttccag aaagtggaag aggagatgaa agagcgcgag ctgcagaaga aatatgagtc
     1801 cgagaaaatc gacctgaccc ttccggcaga agagacagcg atcggaaacc ttcatccggt
     1861 taccctgatc cgcaaacagc tcatcgacat ctttgcaggc atgggctttg aggtatacga
     1921 gggaagagaa atcgagaccg attactacaa ctttactgca ctgaatacac cacaggatca
     1981 cccggcaaga gatatgcagg ataccttcta tctctccccg gaattcctgc ttcgtaccca
     2041 gacatcggca ggccagattc atgtcatgga aaataaaaag ccgccgatca agatcctttc
     2101 cccgggacgt gtttttcggt ctgatgacga cgcaacccat tctccgatgt tccatcagat
     2161 ggagggactc gttgtcgaca aaaacatcac cctctgcgac ctgaaaggaa tgctcgacgt
     2221 actcgtacag aagatctacg gcgagggaac cacaacccgt ctccgtccgt cttacttccc
     2281 gttcacggag ccgtccgttg aggtagactg cagctgcttc gcctgcggcg gaaaaggatg
     2341 cagcctctgc aagggaaccg gctggatcga ggtccttgga gcaggtatcg taaacaaacg
     2401 cgtgctggag aactgcggca ttgattcaga agagtacagc ggcttcgctt ttggtatcgg
     2461 cctggagcgt atcgccatgc tgaaatacgg aatcaacaac atcaaactgt tattcggttc
     2521 cgatctgcgg gttttaaatc agattaacga gaaataaaga cgaaacagtt tgaatttgga
     2581 ggtttaatat gttagtaccg ttaagttggc tgaaagaata tgtagatatt gatgttaccc
     2641 cggaagaact ggagaaaaaa ctgttcgacg gcggttttga agtagaagag cgttatcagg
     2701 tgggaaaaga catcagcaat gttgttgtcg gtctggtgga gggctgcgag ccgatcccgg
     2761 agacccatct tcatgtgtgc caggtaaatg caggagaaca cggcgccatg cagatctgct
     2821 gcggcgcaga caacgtgcac accggcggaa aattcccggt tgcccttcct ggcgcttctg
     2881 tttatgcaac agcaaaagat cataagacca tcgagggtgt tatgaccatc aaaaaaggaa
     2941 aattaagagg atatgagtcc tgcggaatgc tctgctccgg cgtggagctc ggccttaccg
     3001 aggatctgta cccgggcgca ggctacaacg gactgcttgt tctgccggaa gatgccgagc
     3061 ttggcgcaga tgtcaaagag ctgacaggtc ttgacgactg gatctttgat atctccctga
     3121 ctgcaaacag accggactgc cagagcatcc tcggtatcgc aagagaagta tcggcgatgc
     3181 tggaaaaacc tttaaaaatg ccggcaaccg attacaccga gacggatgtg gaaaaagaag
     3241 gcttctctgt ccgcgtggaa gctccggaac tctgcccgcg ttacagcgct cattacgtat
     3301 atgacgtaaa aatcggagag tccccggcat ggatgaaacg ccgtctggca ctcgtcggca
     3361 tgtccgcgat ctccaacgtg gttgatatca caaactacgt cttaaaagag atcggccagc
     3421 cgatgcatgc ttttgactgc tcttatctgg aaggcaatga gatctgcgtc agaagagcaa
     3481 aagaaaatga aaagatcgtg accctcgatg agcaggaata cacaatgaat gagaacaacc
     3541 tcgtgatctg cgacggcgta aaaccggttg cacttgccgg tgtcatggga ggcttaaact
     3601 ccgagatccg cgacacgaca gaggctgtta tgtttgagtc cgcaaaattc gcacgcgaca
     3661 acgtcagaaa gacagcccgt gcgcttggaa aagcgacgga tgccagtgca agatatgaga
     3721 aaggcgtgga cgagtattca accgtacttg gtatgaaacg tgccctgcat ctgatggaag
     3781 agcttggctg cggtaaagtt tcccgtaccc atttcgatgt gaacaccgga aactccatcg
     3841 atccgactcc gatgaccgtc agtgtcagca aagtaaacgg cgtactcggt atcgaagttc
     3901 cggaagcaga gattctccgc attatgaaaa acctgaactt tgcaccggaa atcaatggcg
     3961 atgagctgac catccaggtt ccggcttacc gtgaggatat gctgccggag ggagagaacg
     4021 atgtcgagcg ctatccggac gtggcagagg aagtcatccg tatgtacggc tacgatcacg
     4081 taaccgatac cttcctttcc gctgcacagg taacgatggg cggctacaat gaagagcaga
     4141 aaggcgagct ggcactgaag aataccttat gcaccatggg tatctacgag tgcatgcatt
     4201 actccttctt ctcccctgca gatctggatc ttttgaaact gccggcagat gcaaaagaga
     4261 gaaatgccat cgagatcatc aacccgatca accaggatct gtccctgatg cgtaccacac
     4321 tggcagcttc catgctgaat gccatttcca gaaatgagaa acagggcacc cttgaaggaa
     4381 gactgttcga ggtagcaagc atctacattc cggagtccct gccgctgaca tcctacccgg
     4441 atgagagaaa agttctctgc gtcggaacct tcggaaacaa cgagagcttc ttcacgatga
     4501 agggcatcgc caacaccatc gccgagagca tggacattga gttcacctac gagccggttc
     4561 agaaatcctt cctgcatccg tatcaggcag tgaaagtact ctgcgagggt acagaaatcg
     4621 gatacttcgg aaaagcagca tacgatatcc agaacgagct gagcatgcgt acttccgctt
     4681 acatcatgga gcttgactta aaagagctct ccagatggta cggcaaaaaa cgcaccttcc
     4741 agccgatctc caaattccag gaggagaaac gtgacctggc atttgtcatg gacaaaaaca
     4801 tcagctgcgg cgatgtagag aactgtatcc gcgaggcaaa caaatacgta aaagagatcc
     4861 gtctgttcga tgtttacgag ggcggccaga ttccggaagg caagaagagc atggcgttca
     4921 ccgtcacctt tgcaccgaag gaagaagcat ttgacttcga aaaagtacag aaatttgtcg
     4981 agaagatctg caagaaactg cagaatgagt tcaacatcga actgagaggc tgatttttat
     5041 gaaactttat atcatgcgcc acggtgaaac cgcgtggaac gtagaaggca gactgcaggg
     5101 ccagacggac accgagctga atgagaacgg tgtccgtctg gcgaaagtga cggcggaagg
     5161 actgaaaaat atcccgtttg accttggaat ttccagtcca ctgcgccgcg caaagcacac
     5221 ggcagagctc gtccttgcgg gcagaaatgt gccgcttacg accgacgacc gcctgatgga
     5281 gctttccttt ggctcctggg aagggcttgg atgccgggca aacaattttg agattccgtc
     5341 tgaacacttc gacgattttt acagagatcc gttccacttc cgcccggcgg gagacggaga
     5401 gacgatccag gaggtctgcg accgggcaga agcgttctat catgagctga ctgcaaaacc
     5461 ggagctccag gacaagacga tcctgatcgc cacccacggc tgctgcatgc gtgcgctgct
     5521 gcgcaatgtc tatgaagaca agaacgactt ctggcacggc ggcgtgccgt acaactgtgc
     5581 ggtcagcatc atcgaagtac agaacggtgt ttccacgctg cttgcggata ataaaatcta
     5641 ttatgatccg tcggactgcg taaactttta taaagaagta aaataaaaaa tgatattcag
     5701 aggcagacat aaaaggaaag tatttccctt tgtgcctgcc tctttaaatc gcgtttgccg
     5761 tctgatagaa agtgtgatac tatagaaata acggatttta acaccagtag acaagaagtc
     5821 ccccgtttct gaagcggtgg gatgaattgc taaaagaaac ataacggcag tggcgggcgg
     5881 agagaaatcg gtatcctcca ctgac
//
LOCUS       DAOMUF010000130         5826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3729_length_5826_cov_3.589673, whole genome shotgun sequence.
ACCESSION   DAOMUF010000130 DAOMUF010000000
VERSION     DAOMUF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3729_length_5826_cov_3.589673"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="PLN77_07850"
     CDS             <1..1940
                     /locus_tag="PLN77_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HRM44220.1"
                     /translation="ISENFSTVKGELTSQETDGRWTLANTASSWIGLKQDNRAGETAL
                     ERKAEPAAAMAQVFGKTYPEDQMTYSWKKLMQNHPHDSICGCSVDEVNEEMKTRFAKS
                     RQVADAIYEESVEYLTNKVNTAALPGDGEKIPFVVWNTSGTAKTQVVEKEIHVFRDYN
                     LFVWDGYEAAEQVELPAMVLRDADGNEVPAKIADAGIAFGYDLPDDRFRQPYMAKKVR
                     VTFEAEVPALGYRTYYLETAEQLQNVDVVSGDANVLENDAMKVVVNEDGSYSLLDKKT
                     GRTYENLGCYEDTGDMGNEYIYIQDTGKQVISTKGRKAEVSCVERNAFRTVVEIRHKM
                     MVPSGMGEELQRQREMCIDPYTRVANRSFELVEMDVKTVLTLEKSAKGLRVATTICNQ
                     AKDHRVRVIFPTGLHTSMHMADSAFEVVRRNNRHNDTWTNPCGCERQQCFAAMEDEKG
                     GLLVANRGLYEYEILEEQDNAIAVTLLRCVAEMGDWGYFPTPKAQQLGTFCLEFEVVP
                     YAAGETGTAFEEGYAFQQDLTVAQAGLERAFLRKPGQVKPELIEGKLPLEMSFLAFEG
                     NGIHMTAFKKGQKKDDLFVRFVNHMEQGEILSFKKEDWMKEVYRSNVIEEKDNVLTPD
                     ADGIYHVSLREFEIATFGVVR"
     gene            2076..4286
                     /locus_tag="PLN77_07855"
     CDS             2076..4286
                     /locus_tag="PLN77_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRM44221.1"
                     /translation="MKKWTDRMETPIQAELICYDVNGIHIVFGVTKEKALKLFHFSSA
                     PFDAENLCRKPDNWWPMEGMREAQIEESFQLVQVNFSGYNRPYEKHGNKYIVTAPGFM
                     LKFDSISEERNEDGDLLRIVQKDDVTGARVESVMQFYDGLPVARMYNTVTNEGSENQT
                     LEYISSFSYWGIEKEQKEGIPEFISSDDKMSMIVVHNGWQKELTVKKYRFADLGMAQT
                     QPHNYQRTSKTIEITNTGNWSAKEYIPMGYIGNSAADASLFFEIDHSGSWHYEIGDQN
                     CHFYLAVSGPTEIQSHWSKNLKPGDSFTTVPVAVGVGHDDFEEAIGTLTKYRRRIRRP
                     NKDNENLPVIFNDYMNCLFGDPTTEKEFPLVDAAEKAGCEYFVIDAGWYADGNWWDSV
                     GEWQESRKRFPNGVREVTDYIRSKGMIPGVWLELEVMGINCKKASILPDECFFLRHGK
                     RVYDRSRYQLDFRHPLVVEHVTEVIDRVVRDYGVGYIKMDYNIEPGIGTEVDADSFGD
                     GLLEHERAYLAWLDGIYRKYPDLVIENCSSGGLRMDYAMLARNSIQSTSDQEDYRNYA
                     TISANAAIGVTPEQAAIWSYPLRDGTEEEVIFNMVNALLLRIHQSGHLAEISPERFAL
                     VKEGIDCYKEIRSGIKDGVPFWPMGWADNEDKHLAAGIRVPGDVIYLGVWRRGGETDF
                     EVPLDRAFPGKELEVSCIYPKACGDVFHYDDYSKKLKVHFPETYMARLFQIKVK"
     gene            4412..4936
                     /locus_tag="PLN77_07860"
     CDS             4412..4936
                     /locus_tag="PLN77_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRM44222.1"
                     /translation="MRILTVLSDLMVPLLIFYVVGLGLLMKQDVFGDFLNGAKDGMRT
                     VIGIVPTLIGLMAAVGVLRSSGFLEDFGNLLGTVLEPTGIPSALVPLSVVRLFSNSAA
                     VSLLLDLYKEAGCDSFAGRAASIIMSCTETVFYTMTVYFGAVRIKKTRYTLAGALFSS
                     LVGMTVGVLLARLI"
     gene            5071..>5826
                     /gene="cysK"
                     /locus_tag="PLN77_07865"
     CDS             5071..>5826
                     /gene="cysK"
                     /locus_tag="PLN77_07865"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRM44223.1"
                     /translation="MAKVYDSILDLVGKTPLVELKRIEEKEGLQAKLIAKVESFNPAG
                     SVKDRIAKAMIEDAEAKGLLKEGSVIIEPTSGNTGIGLAAAATVKGYRMILTMPETMS
                     VERRNIVKAYGAEVVLTDGTKGMKGAIEKADELAKEIPNSFIAGQFVNPANPETHKKT
                     TGPEIWEDTDGAVDIFVAGVGTGGTITGTGEYLKEKKPEVKVIAVEPASSPVLSEGVS
                     GPHKIQGIGAGFVPETLNTGIYDEIIKVENEDAF"
BASE COUNT         1583 a         1108 c         1771 g         1364 t
ORIGIN      
        1 aaatctctga gaacttctct acggttaagg gtgagctgac aagccaggaa accgacggaa
       61 gatggacgct ggcaaataca gcgtcttcct ggatcggatt aaaacaggat aaccgcgcgg
      121 gagagacggc actggagcga aaagcagagc ctgcggctgc aatggcacag gtgtttggga
      181 aaacatatcc ggaggaccag atgacctaca gctggaagaa gctgatgcag aatcatccgc
      241 atgacagcat ctgcggctgc agtgtggacg aggtcaacga agaaatgaag actcgttttg
      301 caaagagcag acaggttgcg gatgcaattt acgaggagag cgtggagtat ctgacgaata
      361 aggtaaacac ggcggcactt ccgggggacg gcgagaaaat cccgtttgtg gtatggaata
      421 catcgggaac agcgaaaaca caggtggtgg agaaagaaat tcacgtgttc cgggattaca
      481 atctgtttgt gtgggatggc tatgaggcgg cagaacaggt ggagcttccg gcaatggtgc
      541 tgcgcgatgc ggacggaaat gaagttccgg cgaagattgc ggatgcgggg attgcgttcg
      601 gatacgatct gccggatgac cggttccgtc agccatatat ggcgaagaag gtacgggtga
      661 cgtttgaggc agaggttccg gctcttggat accgtacgta ttatctggaa accgcagaac
      721 agctacaaaa cgtggacgta gtttccggtg acgcgaatgt gcttgaaaat gatgcaatga
      781 aggttgttgt gaacgaggat ggttcttaca gccttctgga caagaagacg ggcagaacgt
      841 atgagaacct gggttgctat gaagacaccg gggatatggg caatgagtat atttatattc
      901 aggataccgg aaaacaggtg atttccacga agggcagaaa ggcggaggtt tcctgcgtgg
      961 agcgaaatgc gttccgtaca gtggttgaaa tccgccacaa aatgatggtt ccgtccggaa
     1021 tgggtgaaga gctgcagcgg cagcgcgaga tgtgtattga tccatatacg cgggtggcaa
     1081 accgctcttt tgagctggtt gaaatggatg tgaagacggt gcttacgctg gaaaaatcgg
     1141 cgaaagggct gcgtgtggct accaccatct gcaatcaggc gaaggatcac cgggttcgtg
     1201 tgatttttcc gacgggactg catacatcga tgcatatggc ggattctgcg tttgaggtgg
     1261 ttcggagaaa taaccgtcat aacgatacct ggacaaatcc atgcggatgt gagagacagc
     1321 agtgttttgc ggcgatggag gacgaaaaag gcggccttct ggttgcaaac cgcggattgt
     1381 atgagtatga gattctggaa gagcaggaca atgcaattgc ggtgacgctg cttcgctgtg
     1441 ttgcggagat gggcgattgg ggatatttcc cgacaccgaa ggcgcagcag cttggaacat
     1501 tctgtctgga gtttgaggtt gtgccatatg cggccgggga gaccggcact gcatttgagg
     1561 aaggctatgc gttccagcag gatctgacgg tggcacaggc cgggctggag agagcgttcc
     1621 ttcggaaacc gggtcaggta aagccggagc tgattgaagg taaacttccg ttagagatga
     1681 gttttcttgc atttgaaggc aatggtattc atatgactgc atttaagaaa ggtcagaaga
     1741 aggacgatct gtttgtgcgg ttcgtgaatc atatggagca gggagagatc ctttctttta
     1801 agaaagaaga ttggatgaaa gaggtttatc gcagcaatgt catcgaagag aaggacaatg
     1861 tgctgacccc ggatgcggac ggtatttatc atgtgtcgct gagggaattt gagattgcta
     1921 cgtttggggt tgtgagataa gcggacgctt cggcaggagg gcgtgcggtc gttgcaaaaa
     1981 acggggaaaa agcaagaata aggtggactt tagggcagac aagttatatg cttttggtat
     2041 aagtaaaaaa tgatttttta caggagggtt tttacatgaa aaaatggaca gaccgaatgg
     2101 aaactccgat tcaggcggag ctgatctgtt atgatgtgaa tggaattcac attgtgtttg
     2161 gggtgacaaa agagaaggcg ttaaagctgt ttcatttttc gagtgcgccg tttgatgcag
     2221 agaatctctg ccggaagccg gataactggt ggccgatgga gggaatgcgg gaggctcaga
     2281 tcgaagagag cttccagctg gtacaggtga atttttccgg ttataaccgg ccgtatgaaa
     2341 agcacggaaa taagtacatt gtgacagcgc ctggctttat gctgaaattt gactcgattt
     2401 cggaagaaag aaacgaagat ggcgatctgc ttcgcattgt acagaaggat gatgtgacgg
     2461 gagcaagggt agagtcggtc atgcagtttt atgacggtct tccggttgca agaatgtaca
     2521 ataccgtgac gaatgagggc agtgagaacc agacactgga atatatctca agcttcagct
     2581 actggggcat cgaaaaagag cagaaagagg gcattccgga gtttatcagc tcagatgata
     2641 agatgtcgat gatagtggtt cacaatggct ggcagaagga gctgacggtg aaaaaatacc
     2701 gctttgcaga tcttggcatg gcgcagacgc agccgcacaa ctatcagaga acatcgaaga
     2761 cgattgagat tacaaatacg ggtaactggt cggcgaagga atatattccg atgggatata
     2821 ttgggaacag cgcagcagat gcgagcctgt ttttcgagat cgatcacagc ggttcctggc
     2881 attatgaaat tggagatcag aactgccact tttacctggc tgtgagcggc ccgacggaaa
     2941 tccagtcgca ctggtcgaaa aacctgaagc cgggagacag ctttacaacc gtgccggtgg
     3001 ctgtcggtgt cggtcatgat gattttgagg aggcaatcgg taccttaacg aagtaccgca
     3061 gaagaattcg ccgtccgaat aaggacaatg agaacctgcc ggttattttc aacgattaca
     3121 tgaactgtct gtttggagat ccgacgacag aaaaagaatt tccgctggtg gatgctgccg
     3181 aaaaagcagg atgtgaatac tttgtcatcg atgccggctg gtatgcggac ggcaactggt
     3241 gggacagtgt cggcgagtgg caggagagca gaaaacgttt cccgaacggc gttcgtgaag
     3301 tgacagatta tatccgttcc aaaggtatga ttcccggggt atggctggag cttgaggtta
     3361 tgggtattaa ctgtaaaaag gcttctattc tgccggatga gtgtttcttc ctgcgccatg
     3421 gaaaacgtgt gtatgacaga agccgttatc agcttgactt ccgtcatccg ctggtcgttg
     3481 agcatgtaac ggaagtgatc gaccgcgtgg tcagagatta tggtgtcggc tatattaaga
     3541 tggattacaa tatcgaacct ggtatcggta cggaagtgga tgcagacagt tttggagatg
     3601 gtctcttaga gcatgagaga gcatatctgg catggctgga tggcatttac agaaaatacc
     3661 cggatcttgt gatcgagaac tgctcaagcg gcggtcttcg gatggattat gcgatgctgg
     3721 caagaaacag tattcagtcc accagtgatc aggaggatta ccgtaactac gcgacgattt
     3781 cggcgaatgc ggcgattggt gtaacgccgg agcaggctgc gatctggtcg tacccattgc
     3841 gggacggtac cgaagaggaa gttattttca acatggtcaa cgcgcttctg cttcgtattc
     3901 accagagcgg tcatctggca gaaatttcgc cggagcggtt tgcactggta aaagagggaa
     3961 tcgactgcta caaagagatc cgcagcggaa tcaaggatgg tgttccgttc tggccaatgg
     4021 gatgggcgga taatgaggac aagcatcttg cagccggaat ccgggtaccc ggagatgtga
     4081 tttaccttgg tgtatggaga cgcggcggag agacggattt tgaagttccg cttgaccgtg
     4141 cctttccggg aaaagagctg gaagtaagct gtatttatcc gaaagcgtgc ggagatgtat
     4201 tccattacga tgattacagc aaaaaactga aggtccattt tccggagacg tatatggcgc
     4261 ggctgtttca gattaaagtg aaataaagta acgattgaga gccccatttc gcatggcgct
     4321 gaatcgttac gaaataaaaa cataaatccc cacccacgga ataaattgta tggagtgtat
     4381 cttcagcaat gttttccggg gaggggattt tttgcgtatt cttacggtac tttcagatct
     4441 tatggttccc ctgctgatct tttatgtggt cggattgggg cttttgatga agcaggatgt
     4501 gttcggagat tttttgaacg gggcaaagga tggcatgcgg actgtgatcg ggattgtgcc
     4561 gacgctgatc gggctgatgg cggcggtcgg ggttttgcgg tcatcgggat ttctggagga
     4621 ttttggaaac ctgctcggaa ctgtgctgga accgacggga atcccatctg cgctggttcc
     4681 gctcagtgtt gtgcgtctgt tttccaacag cgcggcggtg agccttttgc tggatcttta
     4741 caaggaggcg ggctgcgact cctttgccgg acgggcagct tcgattatca tgtcgtgtac
     4801 cgagaccgtt ttctatacga tgacggttta ctttggtgcg gttaggatca aaaaaacgcg
     4861 gtatactctg gctggcgcct tgttttcttc tcttgttgga atgacagtcg gtgttttgct
     4921 tgcacggttg atctgaccat tttttctgat cataccagtg aaatgtgttg acatttcaaa
     4981 acgattggaa taagatggaa acaacttaaa acatacaaaa tcaataggaa ataaggaaat
     5041 aaaaggtgca gaataaggag gaaatgaacg atggcaaagg tatatgacag cattttagat
     5101 cttgtaggaa aaactccgct ggtagagctg aaacggatcg aagaaaaaga aggactgcag
     5161 gcgaaactga tcgcaaaagt agaatccttt aatccggcag gaagtgtcaa agaccgtatt
     5221 gcaaaagcga tgatcgagga tgcggaagca aaaggtctct taaaggaagg ctccgttatc
     5281 attgagccga cctccggaaa taccggaatc ggactggcag cggcagcaac ggtaaaaggc
     5341 tatcgcatga tccttactat gccggaaacg atgagtgtag agcgccggaa tatcgtaaag
     5401 gcatacggcg cagaggttgt tctgacggat ggaacaaaag gaatgaaggg cgctatcgag
     5461 aaagccgatg agcttgcaaa agagattccg aacagcttta tcgcaggaca gtttgtgaac
     5521 ccggcaaacc cggagacaca caaaaagaca accggaccgg agatctggga agacaccgat
     5581 ggagcagttg atatctttgt tgctggcgtt ggtaccggtg gaacgattac cggaacgggt
     5641 gagtacttaa aagagaaaaa accggaagta aaagtgatag cggtagaacc ggcatcctca
     5701 ccggtgctgt cggagggtgt ttccggacca cataagattc agggaatcgg tgcaggcttt
     5761 gtgccggaaa cgctgaatac cggcatttac gatgagatta tcaaggtaga aaatgaagac
     5821 gctttc
//
LOCUS       DAOMUF010000131         5792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3763_length_5792_cov_3.532508, whole genome shotgun sequence.
ACCESSION   DAOMUF010000131 DAOMUF010000000
VERSION     DAOMUF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3763_length_5792_cov_3.532508"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..88)
                     /locus_tag="PLN77_07870"
     CDS             complement(<1..88)
                     /locus_tag="PLN77_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRM44224.1"
                     /translation="MSEITRDEAFTLLKKYNKDPFHIQHALTV"
     gene            complement(289..>1614)
                     /locus_tag="PLN77_07875"
     CDS             complement(289..>1614)
                     /locus_tag="PLN77_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006770817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HRM44225.1"
                     /translation="GEFPHDGNFCMDGLVYPDRTPHTGLMEFKNVYRPVRVTGYDAEK
                     GTLTIKNYMNFVNLKDYAVLGYEVLVDGEVTESGKITDEALLELAAGCEKTIPLAISV
                     SEQGKCALKVTWYQKQATEVLPEQFELGFEEVPVKTKDNQNQKAKEMMKRPEGTASFG
                     WKEDDHYLTVSTEQFSYTYNKFTGLFEEMCYANQNLLDRPMELNIWRAPTDNDRNIKN
                     TWMRAQYDRTVTRAYETTAKEENGCVEIETSYSISSPALQRILAGVIKWTIYSDGTTD
                     VHVEAKKDPVFPVLPRFGLRLFLPESFAELTYCGLGPVESYVDKHQASYHGVFHSTPA
                     EQQEDYIRPQENGSHYDCDYASLASDRAVLTAVGEKTFSFQASIYTQEELTAKAHNYE
                     LRPCGSTVFCIDYRQAGIGSASCGPMLLEKYQLKETEISFDVRLKPELL"
     assembly_gap    1615..1624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1625..3380)
                     /locus_tag="PLN77_07880"
     CDS             complement(<1625..3380)
                     /locus_tag="PLN77_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRM44226.1"
                     /translation="MIVPKYFEDFDHLHVNTMPNRAYYIPASRRMEDLVENREASDRF
                     FLLSGDWKFRYFTSVYDVKEEFFAEGYDTSAFETIPVPSVWQNYGHDHHQYTNVRYPF
                     PVDPPYVPYENPCGAYVRTFEWKKQEEAPRAFLNFEGVDSCFYVWMNGSFVGYSQVSH
                     STSEFDVTDFLKDGTNTIAVLVLKWCDGSYLEDQDKFRMSGIFRDVYLLARPEKGIRD
                     YFVKAAPDASYQNGEVSIAITWNDGEPQEGTAKLFDAENHLVAEAAFGGDTVQMHVKD
                     AHLWNAEEPYLYTLVLETAGEVINDHVGIREIHAKDGVLYLNGVKIKFHGTNRHDSDP
                     VTGFAISLAQIKKDLKVMKEHNINAIRTSHYPNAPYCYQLYDRLGFYVIDEADNESHG
                     TEEIYANYTSWEEYAKNWNKLIANNPVFTEATVDRTQRCVERDKNRPSVVIWSMGNEC
                     AYGCTFEAALKWTKEFDPTRLTHYESARYVDDPKKYDYSNLDLYSRMYPSLEEIKKEL
                     VEYGDKPYIMCEYCHAMGNGPGDLEDYFELIHSEEKMCGGFIWEWCDHAIDMGKTIEG
                     KKMYAYGGDHNEYPHDGNF"
     gene            complement(3475..5724)
                     /locus_tag="PLN77_07885"
     CDS             complement(3475..5724)
                     /locus_tag="PLN77_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM44227.1"
                     /translation="MEKTTKNGIHRIRQEGGKTIAWAEESGVKVLEKDGYYFKDLAKT
                     GELLPYENWRLSDEERAADLAGRLSIEEIAGLMLYSPHQAVPPMPGGPFQGTFDGKTY
                     LESGKEPYAISDQQKEFLEDEHIRHILLTNVESPEISAKWSNELQKRAETLPYGIPIN
                     LSSDPRNGAKDSGAEFKSGGSEISKWPEGVGFAACFDPEVAGQFAKDASREYRALGIT
                     TALGPQIDLCTEPRWMRFVDTLGEEVEMSKKLTKAYCDGMQTTEGEADGWGKDSVNTM
                     VKHWPGGGTGEAGRDAHYAFGQFAVYPTGNFEEHLKPFTEAAFHLDGPTDCASAVMPY
                     YTVSYGVDKKNGKNVGNSYSEYLIKDLLRGKYEFKGIVCTDWGITQDPEKTIEGFGSR
                     CYGVQDMTEAERCLLAITNGVDQFGGNSESGPIVEAYKIGCEKYGEKAMRERMELSAK
                     RLLINIFHCGLFEDPYLDPEESAKIVGCEEFCRHGYEAQQKSIVLLKNSAKRAPEGQK
                     GVLPLKKGLKVYIPERKIGPSKAFFRIDLPAKTEDPLPDGLPSKYGTRVSSPEEADVA
                     LVFIESPACNPYSTEDLANGGNGYLPITLQYRPYTAKKAREVSIAGGDFRENFTNRSY
                     LGKTNTAYNEADLDNILECRRAMGDKPVIVCATVNNPMVMHEFEAEADAIVAEFGVSR
                     AAVLDVVFGGYNPTGRLPIQMPKDMDAVEEQSEDRALDMETYIDSEGHNYDYGYGMNY
                     EGVLPAWKK"
BASE COUNT         1168 a         1660 c         1355 g         1599 t
ORIGIN      
        1 ctacggtaag cgcatgctga atatggaagg gatctttgtt gtattttttt aataaagtaa
       61 atgcctcatc tcttgtaatt tcactcatgc ttgattttct ccttcccttt tctctttcca
      121 gaatcacctg gattagattt gaattataat ttcgtccatt ataatcctgt attcatagta
      181 attcaatagg tttatcaaga cttttttgta catcatataa aaaggcgccg ttccctcacc
      241 tttcagagag agggcacagc gcttttctta tttttcaaat tctacacttt acagcagttc
      301 cggtttcagc cgcacatcga agctgatttc ggtttctttc agctgatatt tttccagcag
      361 catcggtccg cagcttgccg atccgatccc ggcctggcgg tagtcgatgc agaaaacagt
      421 gcttccgcac ggccgaagct catagttgtg ggctttcgcg gtcagctcct cctgcgtgta
      481 aatagatgcc tggaatgaga aggttttctc gcccactgcc gtcaataccg cacgatcact
      541 ggcaagactt gcataatcgc agtcgtagtg gctgccgttc tcctgtgggc ggatatagtc
      601 ctcctgctgt tctgccggtg tgctgtggaa aacgccgtga tagcttgcct ggtgcttatc
      661 cacgtagctc tccaccgggc cgaggccgca gtaggtcagt tctgcaaacg attccggcag
      721 gaacaggcgc aggccaaaac gcggcagaac cgggaatacc gggtctttct ttgcttctac
      781 atggacatcg gtcgttccgt cgctgtaaat tgtccatttg atcacacctg caaggatccg
      841 ctgcagagcc ggcgacgaaa tcgaatagct tgtctcgatc tccacgcagc cgttttcttc
      901 ttttgccgtt gtctcatacg cccgcgtcac ggtgcggtca tactgagcac gcatccaggt
      961 attcttgata ttgcggtcat tgtctgtcgg cgcacgccag atgttcagct ccatcgggcg
     1021 gtcgagcaga ttctggttgg catagcacat ttcctcgaac aggccggtga acttattata
     1081 ggtatagctg aactgttccg tcgataccgt cagataatga tcgtcctctt tccatccaaa
     1141 cgaagccgtt ccctccgggc gcttcatcat ctcttttgcc ttctggttct gattatcttt
     1201 cgttttcacc ggaacttctt caaatcccag ttcgaactgc tccggcagca cctcagtggc
     1261 ctgtttctga taccaggtaa ccttcagcgc acattttccc tgctccgaca cggaaatggc
     1321 aagcggaatc gtcttctcac agcctgccgc aagctccagc agtgcttcat ctgtaatctt
     1381 gccgctctcc gtcacttcgc cgtccacaag cacctcatag ccgagcacag cgtaatcttt
     1441 cagattcaca aaattcatat agtttttgat ggtcagtgta cccttttcgg catcatatcc
     1501 cgtcacgcgc accggacgat acacgttttt aaattccatc agtcccgtat gcggtgtgcg
     1561 gtccggataa acaagtccgt ccatgcagaa atttccatca tgtgggaact caccnnnnnn
     1621 nnnnagaagt tgccgtcgtg cggatactcg ttgtggtcgc cgccgtacgc gtacatcttc
     1681 tttccctcga tcgtttttcc catatcaatc gcgtggtcgc accactccca gataaagccg
     1741 ccgcacatct tctcctcgct gtggatcagt tcaaagtagt cttcaagatc tcccgggccg
     1801 ttgcccatcg catggcagta ttcgcacatg atatacggct tatccccgta ctccaccagc
     1861 tcttttttga tctcctcgag tgatggatac atgcggctgt agaggtcgag gttgctgtaa
     1921 tcgtatttct tcggatcatc cacataccgt gccgactcat agtgcgtcag acgcgtcgga
     1981 tcaaattcct tcgtccactt taaggccgcc tcgaaggtac agccgtatgc gcactcattt
     2041 cccatcgacc agatgacaac gctcgggcgg tttttatcgc gctctacgca acgctgtgtg
     2101 cggtctacgg ttgcctcggt aaataccgga ttgttcgcaa ttagtttatt ccagttcttc
     2161 gcatactctt cccagctcgt gtaattcgca taaatttcct ctgtgccgtg gctctcattg
     2221 tccgcctcat cgatgacata aaaaccgaga cggtcataaa gctgataaca gtacggcgca
     2281 ttcgggtaat ggctggtccg gatcgcattg atgttgtgct ctttcattac cttcagatct
     2341 tttttgatct gtgcaaggct gattgcaaat cccgtcaccg gatcgctgtc gtggcggttt
     2401 gttccgtgga atttgatctt cacgccgttc aggtacagca caccgtcctt cgcgtggatc
     2461 tcacggatgc cgacatgatc attgatcacc tcacccgccg tctccagcac cagcgtatac
     2521 agatacggct cctcggcatt ccacagatgt gcatctttta catgcatctg aaccgtatcg
     2581 ccgccaaacg ctgcctctgc cacaagatga ttttctgcat caaacagctt tgccgttcct
     2641 tcctgcggtt ccccgtcatt ccaggtaatc gcaatcgaaa cttctccatt ctggtacgat
     2701 gcgtccggcg ctgcctttac aaagtaatcg cggatgcctt tttctggtct tgcaagcaga
     2761 tagacatcgc ggaaaatgcc gctcatgcgg aatttgtcct gatcctcgag atagcttcca
     2821 tcgcaccatt tcagcaccag tacggcgatg gtgttggtgc catcttttaa gaagtctgtt
     2881 acatcaaatt cactcgtaga atgagacacc tggctgtagc cgacgaagct gccgttcatc
     2941 cacacgtaga aacaggaatc cacgccctca aaattcagga atgccctcgg tgcttcctcc
     3001 tgttttttcc actcgaacgt tctcacgtac gcaccgcacg ggttctcata cggcacatac
     3061 ggcggatcca ccgggaacgg gtagcggacg ttcgtatact gatggtggtc atggccataa
     3121 ttctgccaga cggacggcac cggaatcgtc tcgaatgcag aagtgtcgta tccttctgcg
     3181 aaaaactcct cttttacatc gtaaacactg gtaaaatagc gaaatttcca gtcgccggaa
     3241 agcaggaaaa agcggtccga cgcctcccgg ttctccacca gatcctccat gcggcgggat
     3301 gccggaatgt agtaggcacg gttcggcatg gtattcacat gcaggtgatc gaaatcttcg
     3361 aaatattttg gaacaatcat ctctttttaa gtcctcctta ttctgtcttt tgtttccaat
     3421 atcggcaccg aaacacacgc ggtcccggtg ccgatcaggt tttctgttat tcttttactt
     3481 tttccatgca ggaagaacgc cttcgtaatt catgccataa ccgtagtcat agttatgtcc
     3541 ttcgctgtca atataggttt ccatatccaa cgcacggtcc tcgctctgct cctcaaccgc
     3601 atccatatcc ttcggcatct ggatcggcag acggccggtc gggttgtaac cgccgaagac
     3661 aacgtcgagc acagcggctc tcgaaacacc gaactctgcg acgatcgcat cggcttcggc
     3721 ttcaaattca tgcatcacca tcgggttgtt gacggttgcg cagacgatca ccggcttgtc
     3781 gcccatcgcg cggcggcatt cgagaatatt gtcgagatca gcctcgttgt aggcagtgtt
     3841 tgtctttcca agatagctgc ggttcgtgaa attctcgcgg aaatcaccgc cggcaatgct
     3901 cacctcacgc gcttttttcg ccgtatacgg gcggtactgc agtgtaatcg gcagatagcc
     3961 gttgccgccg ttcgcaagat cttcggtgga atacgggttg catgccgggc tttcgataaa
     4021 taccagcgcc acatcggcct cctccggtga tgatacgcgt gtaccgtact tggacggcag
     4081 tccatccggc agcggatctt cggtttttgc cggcagatcg atgcggaaga acgctttgga
     4141 cggtccgatc tttctctccg gaatataaac cttcaaacct ttcttcagcg gcagcacacc
     4201 cttctgtcct tccggtgcgc gtttcgcact gtttttcagc aggacgatgg atttctgctg
     4261 tgcctcatag ccatggcggc agaactcttc gcatcctaca atctttgcgg attcttccgg
     4321 atcaagatac gggtcctcaa acaagccgca gtggaaaatg ttgatcagaa gacgtttggc
     4381 ggacagctcc atgcgctcac gcatcgcttt ctcgccgtat ttctcacagc cgatcttata
     4441 cgcctcgaca atcggaccgg actcgctgtt tccgccgaac tggtcaacgc cgtttgtgat
     4501 cgccagcaga caccgttctg cctcggtcat atcctgcacg ccgtagcagc ggcttccaaa
     4561 tccctcaatc gtcttctccg gatcctgggt gatgccccag tcggtgcaga caatgccttt
     4621 aaattcatat tttccgcgca gcagatcctt gatcaggtac tcgctgtagg agtttccgac
     4681 gttcttgccg tttttcttgt ccacgccgta ggatacggtg tagtacggca taaccgcgga
     4741 tgcgcagtcg gtcggtccgt ccaggtggaa ggctgcctcg gtaaacggtt ttaaatgctc
     4801 ctcaaaattt ccggtcggat aaacggcaaa ctgtccgaat gcatagtgtg catcgcgtcc
     4861 ggcctctccg gtgccgccgc ccggccagtg tttgaccatc gtgttgacgc tgtcttttcc
     4921 ccagccgtct gcttcccctt ccgttgtctg cataccgtca cagtacgctt tggtaagctt
     4981 tttggacatc tccacttctt cgcctaacgt atcgacaaag cgcatccatc tcggctcggt
     5041 gcagaggtcg atctgcggtc cgagtgccgt tgtgatgccg agtgcccggt attcacggga
     5101 ggcatccttt gcaaactgtc cggctacttc cgggtcaaag catgctgcaa agccgacacc
     5161 ctccggccat ttgctgatct cgcttccgcc gctcttgaac tctgcgccgg aatcctttgc
     5221 tccgttccgc ggatcagagg agaggttgat cggaatacca tacggcagtg tctctgcccg
     5281 tttctgcagc tcattgctcc attttgcgga aatctccggg ctttccacgt tcgtcagcag
     5341 gatatggcgg atgtgctcgt cctccagaaa ttctttctgc tgatcagaaa tcgcatacgg
     5401 ttctttgccg gattccagat acgtttttcc gtcaaacgtt ccctggaacg gaccgcccgg
     5461 catcggcggt acggcctggt gcggtgagta cagcataagg ccagcgatct cctcgatgga
     5521 aagacggccc gcaaggtctg cggctctctc ctcatcggag aggcgccagt tctcatacgg
     5581 cagcagttcg ccggtttttg ccaggtcttt aaagtaatac ccgtcttttt ccagtacctt
     5641 aactccggac tcctctgccc aggcgatcgt ttttccgccc tcctggcgga tccggtggat
     5701 tccgtttttc gttgttttct ccataagttt cacccttctt ttcccaattt attttaatgt
     5761 aaataccgca ctgttttccg tcgtgctgct gt
//
LOCUS       DAOMUF010000132         5789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3770_length_5789_cov_4.892571, whole genome shotgun sequence.
ACCESSION   DAOMUF010000132 DAOMUF010000000
VERSION     DAOMUF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3770_length_5789_cov_4.892571"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLN77_07890"
     CDS             complement(<1..500)
                     /locus_tag="PLN77_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRM44228.1"
                     /translation="MRKKFLALSLAVIVSAAQVMTVSASKQEQLQQQQAAQAETSAQL
                     DNAKAEIDDLETKKNQITGEIDDLDAQLVVTMASVENLKNEIAEKQTEIEDTQKKLSD
                     AQAEEDEQYEQMKKRIKFLYESGGDNGWAALILEGKDLSSILNQAEYTQKLYKYDREC
                     LQEYMDL"
     gene            complement(698..1768)
                     /locus_tag="PLN77_07895"
     CDS             complement(698..1768)
                     /locus_tag="PLN77_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM44229.1"
                     /translation="MRKSWKWALCLGVVSLLLLGGCGKEKTEPVDLVLVTDGSEVASD
                     AVYQSAWNGLAQYGDESGLKYEASVPAGRTTEDYENTIKEAAQKGASVIVCAGTSMSR
                     AVYDAQRDWKDVRFLLLEAEPVSESGRSRLRGNTESLEIDVSEAGYLAGYAAVQAGYT
                     HLGYIGQKNEENGTKYGTGYALGAEAAAADLGLGENSITLDYTYRKSSSVSPSYLEKI
                     KSWYGEGGQILFSDGASYQNVLGAAASAAGGALIANGGVEEYANAVLKVRLDYGTAVY
                     NALKEMKQKDFEGGKIVTRGVTDQEISLEWNQDAVPYFTEDQYRALCAKFSAEDFSIP
                     DGDALSSPDQYKLSCVSVQKVK"
     gene            complement(1934..4678)
                     /locus_tag="PLN77_07900"
     CDS             complement(1934..4678)
                     /locus_tag="PLN77_07900"
                     /inference="COORDINATES: protein motif:HMM:NF040203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44230.1"
                     /translation="MTSKKKLRSLLGGTLFMVLMLSVHPTTVSASAVGSSTVMDQSVE
                     TEDQQSGIQSETQTQASQDSVAAVRASQNGWVKAGDGGWYFYENGQLKTGWLYRNGNW
                     YWLDPDRNGRMAENSWFTYDNNLYYAKGDGAIYKNGFQEVDGNLYYFQSWGGIQRNVY
                     LSISGKMYYVQENGIVARGIVRTMNGHGDLCAFDDTTGAQITQKGWLKKGTSYYWVRE
                     DGVLQTGWLLYDDNWYWFDDNGVMMASGWKEINNNLYYFQSWGGIYKNGFQSIGGNEY
                     YFRSWGGVYRNTFFTVKGKTYIAQNDGSIYHASQTGWVQLGDQTYWINQDGSVQTGWL
                     KLEGKWYWLKADGTRAASEWITYDNNRYYFDGDGVMYTGMKEVDGTRYYFHSWGGVYH
                     NESFTWQGKKYWAKDDGTFYTGVCDINGKKYYFLEDGEQITKEGWYLTNGTYYWINKD
                     TSLQTGWVKYDNNWYWMKEDGTMASSEWITYDKNRYYFRSWGGMYTGIHTIGGTKYAF
                     QSWGGLYHDQTFTIGGKTYYANSDGTFATGWVQNGGKTYYFDEDGTSHTGWLLLDGTH
                     YWINANGTRRDDELFQYDGNYYYVDKNGVMATSGWVYWDYNYYYPRSWGGMYKNAFIT
                     YDNNLYYLGSDSKMAIGWQSIGGNTYYFRNWGGMITGKQVIDGKTYVFDEDGKLVQSP
                     DGFEPSAQIGVRTVRNFLKNALLPLGNTLYIWGGGHTDAEAESYGVNAQWKQFFNTQN
                     STYNYSDHLYEYGKGLDCSGYVGWTAHQVTKEYATTTSTGMPAYFARKGWGTCVTGDT
                     SQKFTPGDVVSKSGHVWIVIGQCSDGSVVVIHATPPYIQLGGTVSSTGSINSEAIELA
                     NEYMKMYYPVAYERYGVKVLDRSYLTGVNHFTWSSSILSDSEGYRRKKPAEILNDLFQ
                     "
     gene            complement(4866..>5789)
                     /locus_tag="PLN77_07905"
     CDS             complement(4866..>5789)
                     /locus_tag="PLN77_07905"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRM44231.1"
                     /translation="DALKPNLVQTLEGTPAIVHGGPFANIAHGCNSVTATKMCLKLAD
                     YTITEAGFGADLGAEKFLDIKCRMAGLKPNAVVVVATVRALKYNGGVPKAELNGENLE
                     ALEKGMPNLLKHVSNITNVYKLPCVVAINAFPTDTEAELKLVEEKCKELGVNVVLSEV
                     WAKGGEGGVALAEEVVRLCEQPNDFTYAYDLEGSIEEKLNAIVQKIYGGSRVVLTANA
                     QKQAKQLEALGFGNCPICMAKTQYSLTDDQTKLGAPTGFEVTVRNLKISAGAGFIVAL
                     TGDIMTMPGLPKVPAAERIDVDATGKITGLF"
BASE COUNT         1248 a         1570 c         1204 g         1767 t
ORIGIN      
        1 agatccatgt actcctgcag gcactctctg tcgtatttgt ataatttctg ggtgtactca
       61 gcctggttca ggatgctgct taaatctttt ccttccagaa tcagtgctgc ccagccgttg
      121 tctccgccgc tttcatagag gaatttgata cgttttttca tctgctcgta ctgctcgtcc
      181 tcttctgcct gtgcgtcact taattttttc tgagtatctt caatctcagt ctgtttctct
      241 gcaatttcat ttttcagatt ttccacggat gccatggtta cgacaagctg tgcgtccagg
      301 tcatcaatct cgccggtaat ctgattcttt ttggtttcca gatcgtcgat ttctgctttt
      361 gcgttgtcaa gctgtgcgct ggtctctgcc tgtgctgcct gctgctgctg taactgctcc
      421 tgtttcgagg cgcttactgt cattacctga gctgcactta cgatcactgc taaacttaag
      481 gcaagaaatt tttttctcat tctttcactc ccaaattgat tcgttgtatg taggtttctt
      541 tgtttcaggt ttccctgttt cttagaaatt caattattct gtaacaattt tgaaatattt
      601 ttcccaattc ttttacataa tatcacaatt cacaaatgga tgcaaggaaa aatatcccgc
      661 ctttttgtaa acaattcgtg aacagtaacg atacatgtta ttttaccttc tgtacactca
      721 cacaactcaa tttgtattga tccggcgagg aaagcgcatc gccgtcgggg attgaaaagt
      781 cctctgcgga gaatttcgca cagagtgccc ggtactgatc ctctgtaaag tacggcaccg
      841 catcctgatt ccattccaga ctgatttcct gatcggtcac gccgcgcgtt acaattttcc
      901 cgccttcaaa atccttttgt ttcatttctt ttaacgcatt gtaaactgca gttccataat
      961 ccagacgtac tttcaacacg gcattggcgt actcctccac gccgccgttt gcgatcagtg
     1021 ctccgcctgc cgcagatgct gctgcgccaa gtacattctg atacgatgca ccgtccgaga
     1081 agagaatctg tccgccctca ccataccagc ttttgatttt ttccaaatac gacggtgata
     1141 cgctgctgct tttccgatac gtataatcca gcgtgatgct gttttctcca agtcccagat
     1201 ccgcagcggc cgcctctgca ccgagtgcat aacctgtccc gtactttgtt ccgttctctt
     1261 cgtttttctg tccgatatag cccaggtgcg tgtagccggc ctgtactgcc gcatatcctg
     1321 ccaggtagcc ggcctctgat acatcaattt ccaggctttc tgtatttccg cggagacggc
     1381 tgcggccgct ctcactgacc ggttctgcct ccagcagcag aaaacggaca tccttccagt
     1441 cgcgctgtgc atcgtaaacg gcgcggctca tgctcgttcc ggcacagacg ataacggatg
     1501 ctcccttctg cgccgcctct ttgatggtgt tttcatagtc ctctgttgtc cgtcctgccg
     1561 gaacacttgc ttcatatttc agaccgctct cgtctccata ctgcgcaaga ccgttccatg
     1621 ctgactgata cacggcatcg gaagctacct cactgccgtc ggttaccagc accagatcca
     1681 ctggttccgt cttttctttt ccgcagccgc ccagcagcag caacgacact acgccaagac
     1741 agagcgccca tttccagctc tttcgcatgt ttactcccct tgtattttta ttttccagat
     1801 tttttctttt gtatttctga tctgcgtctt tcacgcacct tctagtatag acaattccag
     1861 cgcaaaaaga aagagagaat acacgaacta caaagttttt ttgtattctc tcttattttt
     1921 tatagaagat attttactgg aacagatcgt tcaggatttc tgccggtttc ttcctgcggt
     1981 agccttcact gtcacttaag atactgctgc tccaggtgaa atgattcaca ccggtcagat
     2041 aggaccggtc aagaactttt acaccatacc gctcatacgc aaccggataa tacattttca
     2101 tgtattcatt tgccagctca atagcttcac tgttaatgct tcctgttgaa gaaacggttc
     2161 cgccaagctg aatatacggc ggtgtcgcat gaatgacaac cacgctgccg tccgagcact
     2221 ggccaatgac aatccagaca tgaccgctct tgctgaccac atcacccggc gtaaatttct
     2281 gggacgtatc gcctgtcacg caggttcccc agccttttct tgcaaagtag gccggcattc
     2341 cagtggaggt tgtcgttgca tactctttcg tcacctgatg tgcggtccat ccgacatagc
     2401 cggaacagtc aagacctttt ccgtactcat acagatgatc ggaatagttg taggtggaat
     2461 tctgtgtatt gaagaattgt ttccactgcg cattgacgcc gtaactctct gcctctgcgt
     2521 ctgtgtggcc gcctccccag atatacaggg tgtttccaag tggaagcagt gcatttttca
     2581 gaaagttccg tacggtgcgg acaccgatct gcgcggacgg ttcaaatccg tccggactct
     2641 gtaccagctt gccgtcctca tcgaacacat acgtttttcc gtcgatcacc tgttttccgg
     2701 ttatcatgcc gccccagttt cggaaatagt atgtatttcc gccaatcgac tgccagccga
     2761 ttgccatttt cgagtcagat ccaaggtaat agaggttatt atcataggta ataaacgcat
     2821 tcttgtacat gccgccccag cttctcggat aataatagtt ataatcccag tatacccagc
     2881 cgcttgtcgc cataacgccg tttttgtcta cataataata gtttccgtcg tactgaaaca
     2941 gctcgtcatc tcttctggtt ccattggcgt tgatccagta atgcgttccg tccagaagca
     3001 gccagcccgt atggcttgtg ccgtcctcat cgaaataata cgtttttccg ccgttctgca
     3061 cccagccggt ggcaaatgtt ccatctgaat tggcatagta tgttttaccg ccgatggtaa
     3121 atgtctgatc atggtacaga ccgccccagc tctggaatgc gtattttgtt ccgccgatgg
     3181 tatgaattcc ggtgtacatg ccgccccagc ttcggaaata gtagcggttt ttgtcgtagg
     3241 tgatccattc agacgatgcc atcgtaccgt cttctttcat ccagtaccag ttgttgtcat
     3301 atttcaccca gccggtctga agcgacgtat ccttgttgat ccagtaatac gttccgttgg
     3361 tcagatacca gccctctttt gtaatctgct cgccgtcttc caggaaatag tatttctttc
     3421 cattgatatc gcagacacct gtatagaagg ttccgtcatc ctttgcccag tatttctttc
     3481 cctgccaggt aaaagattcg ttatggtaaa cgccgcccca gctgtggaaa taatatcttg
     3541 ttccgtcaac ctctttcatt ccggtgtaca tcacaccgtc accgtcaaaa tagtatcggt
     3601 tgttgtcata cgtgatccat tcagatgcag cacgtgtgcc gtccgctttc agccagtacc
     3661 atttgccttc cagcttcagc cagccggtct gaacagaacc atcctgatta atccagtaag
     3721 tctgatctcc cagctgaacc cagccggtct ggctggcatg atagatcgat ccgtcattct
     3781 gcgctatgta ggtttttcct tttacggtaa agaaggtgtt tcgatataca ccaccccagc
     3841 tccggaaata atactcgttt cctccgatgc tctggaaacc gtttttataa atgccgcccc
     3901 agctctggaa gtaatacaga ttgttgttga tttctttcca gccggatgcc atcatgacac
     3961 cattatcatc aaaccagtac cagttatcat cgtacagcag ccagccggtc tgcaggacgc
     4021 cgtcttctct tacccaataa tatgatgtgc cttttttcag ccagcctttt tgtgtgatct
     4081 gcgctcccgt agtatcatca aatgcgcaga gatcaccatg tccattcatt gttctgacta
     4141 ttccacgggc aacgatacca ttttcctgta catagtacat ttttccgctg atgctgagat
     4201 acacatttct ctgaatgccg ccccagctct ggaaataata gaggtttcca tctacttcct
     4261 gaaaaccatt tttatagatt gctccgtctc ccttggcata ataaagatta ttatcgtatg
     4321 tgaaccagct gttttctgcc atgcggccgt tccggtccgg atccagccag taccagtttc
     4381 cattccggta aagccatcct gttttcagct gtccgttctc ataaaaatac cagccgccgt
     4441 cgcctgcctt aacccagcca ttctgactgg cccgcacagc agcaacgctg tcctgtgaag
     4501 cctgtgtctg cgtttcactc tgtatcccgc tctgctgatc ctctgtctcc acagactggt
     4561 ccatcaccgt gctgctcccc acagcactcg cggaaacagt cgtcggatgt acgctgagca
     4621 tcagcaccat aaaaagtgta ccgcccagaa gacttctcaa ttttttcttc gatgtcattg
     4681 gcaagtaact cccttactat attatatctg cttttctctc catgttatca ttagaaatta
     4741 tattatcttt tgtaatattt tgcaataaaa acgtaaaaaa agagacattc acgaaggaat
     4801 gtctcttttt ttctgtgttt tatttcacgc tttattctgt cttttcaaga caatatcctg
     4861 tgttattaga acaatcctgt aatctttcca gtagcgtcta catcgatacg ctctgctgcc
     4921 ggaactttcg gcagacctgg catggtcatg atgtcaccgg tcagggctac gatgaagcct
     4981 gcaccagcgg agattttcag gttacgtacg gtaacttcga atccggtcgg tgcgccgagt
     5041 ttggtctgat catcggtcag gctgtactgg gtttttgcca tacagatcgg gcagttgcca
     5101 aatccgagag cctctaactg ttttgcctgt ttctgtgcat ttgcggtcag gacaactctg
     5161 cttccgccgt agatcttctg aacgatggcg ttcagtttct cttcgatgga gccttccaga
     5221 tcgtatgcat aggtgaaatc gttcggctgc tcgcaaagac ggacaacttc ttctgccagg
     5281 gctacgccgc cctcgccgcc ttttgcccat acctcggaca gaacaacgtt gacgccgagc
     5341 tctttacatt tctcctcaac cagcttcagt tctgcttcgg tatcggtcgg gaatgcattg
     5401 attgcaacaa cgcaaggcag tttgtatacg tttgtgatat tgcttacatg tttcagaagg
     5461 ttcggcatac ctttctccag agcttccagg ttctcgccgt tcagttctgc tttcggaacg
     5521 ccgccgttgt atttcagggc acgaacagtt gctacgacta caactgcatt cggtttcagg
     5581 cctgccatac ggcatttgat gtcgaggaat ttctcagcac caaggtcagc accgaagcct
     5641 gcctctgtga tagtgtaatc tgctaatttc aggcacattt tggttgctgt tacagagttg
     5701 cagccgtgtg cgatgtttgc gaatggtccg ccgtgaacga tagccggagt tccttccaga
     5761 gtctgtacca ggtttggttt cagggcatc
//
LOCUS       DAOMUF010000133         5783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3777_length_5783_cov_2.296788, whole genome shotgun sequence.
ACCESSION   DAOMUF010000133 DAOMUF010000000
VERSION     DAOMUF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3777_length_5783_cov_2.296788"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PLN77_07910"
     CDS             complement(<1..1224)
                     /locus_tag="PLN77_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM44232.1"
                     /translation="MEKTKKLAVGILAHVDAGKTTLAEGILYKTGQIRKAGRVDHKDA
                     FLDTEELEKARGITIFSKQAVLKLNETEVTLLDTPGHVDFSAEMERTLQVMDYAVLLI
                     SGADGVQGHVETLWRLLARYEIPVFLFINKMDQPGTDAEKLLEELQSRLSEHCLNFSQ
                     DLQNSELLEELAMCDEDVLEQYLETGSVEEDQIRTMIAERKVFPCCFGSALKMEGVTE
                     FLKILDRFTKTPEYGGDFGARVFKISRDTAGNRLTHLKITGGVLKVKQMLGEEKADQI
                     RIYSGAGYTMVQEAPAGTICAVTGLNSTFSGQGIGNETEAEKPVLEPVLTYRIELPPD
                     CDVHQMLGKLRQLEEEIPELHIVWNERLAEIHAQVMGEVQIEILKSLIHERFGEWVEF
                     GAGNIVYKETIRSTVE"
     gene            complement(1372..>1815)
                     /locus_tag="PLN77_07915"
     CDS             complement(1372..>1815)
                     /locus_tag="PLN77_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44233.1"
                     /translation="AGLWNGAGALRRALGGFSLLRSDFRTDVIEKDPASFQKLLDGYF
                     AAGEKLDKKEALRVAEEYLGQDAATLETSLDWISYQDLAISEEAIYLADRVVIFDANP
                     WQIKAVLTIPLGKHRDRTSEDFLAIRDQIFEIFHMKREDAIEYYI"
     assembly_gap    1816..1825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1826..2033
                     /locus_tag="PLN77_07920"
     CDS             <1826..2033
                     /locus_tag="PLN77_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44234.1"
                     /translation="NDIILIYLPKFKALRHRNVVQRKTCFRQLNQFRCTVDFSGVQCI
                     CQIRPGAEIVNSIRYCFLLSRVQM"
     gene            complement(2110..>2746)
                     /locus_tag="PLN77_07925"
     CDS             complement(2110..>2746)
                     /locus_tag="PLN77_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRM44235.1"
                     /translation="GKEAAEVFAALLKGETVEKTDLLLVAPKCLQNRVLEMIDEEIAH
                     AKQGEESYIGIKINSLTDKAIITKLVEASQAGVKIEMIVRGICCLIPGIEGYTENIKV
                     VSIVGRFLEHSRIYRFGTKEREKVYLASADFMTRNTIRRVEVAAPVLDETLKERLDWM
                     FETMMKDNEKGKCLNASGKYVDRESNGTKLNSQELFYAMAYSNAEKAQTKA"
     assembly_gap    2747..2801
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2802..4209)
                     /locus_tag="PLN77_07930"
     CDS             complement(<2802..4209)
                     /locus_tag="PLN77_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRM44236.1"
                     /translation="MKKQQLKPEPYMMNRELSWLKFNERVLNEAGNPAVPLAERLTFA
                     SIYQSNLDEFYRVRVGTLMDQMESKEVVRENKTNMTSEQQVKAILQETRDLDQKKAAI
                     YEQLMGELEPKGIRLINFNKLSAEEGKLLETYFDNEIAPYLSANIVSKQQPFPFLKNK
                     DIYAVALLETKGGKTRTAIIPCSNNVFRRLIDIPTRKGTFMLSEELILHFLPKMFKNY
                     IVKEKSLIRVTRNADIDTETIYDEDLDYRDAMENLIKQRKRMSPVRMEMSRELNKKLT
                     SSLCKEIKVDKDHVFLSRVPLDLSFVFALQGYLRSLEQNGTADTKQLFYQRRAPRMTP
                     QLDSKAPLIPQVMKKDVLLSYPFESIKPFISLLDEAAKDESVVSIKMTLYRLADKSQI
                     VDALVEAAENGKEVVVLVELRARFDEESNIEYSRILEEAGCRVIYGLNGFKVHSKLCL
                     ISRKTEDGVSYVTQIGTGN"
     gene            complement(4372..5028)
                     /locus_tag="PLN77_07935"
     CDS             complement(4372..5028)
                     /locus_tag="PLN77_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM44237.1"
                     /translation="MTYKEIIKNKEINAYLKKGNENLGQLGYTDHSQKHCMQVAHQAG
                     KILKKLGYSEHEIELAQIAGYMHDIGNVINRSHHAEYGALLANELLKETDMTLEERVT
                     IVSAIGNHDESTGSPEDVISAALIIADKTDVRRSRVRQKEQSAFDIHDRVNYAVTDSK
                     LKVDEKKSEIALNLQIDEKICSMYDYFEIFLGRMMLCRKSAEILGTTFKLTVNGRKVL
                     "
     gene            complement(5053..>5783)
                     /locus_tag="PLN77_07940"
     CDS             complement(5053..>5783)
                     /locus_tag="PLN77_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRM44238.1"
                     /translation="SGGKIVPLVYKKSKPYGVSPRNVGQYFMQEALMTSAEEAPLVIL
                     KGMAGTAKTFYSLAVGMEKVYNNPTGEYRRVIVCRPNAQFDDDIGFLPGDEKEKIAPL
                     MRPVIDNLEQILDSDEKRRYEDEEELGDKITEIFERGIIQTEAMNFIRGRSIARTYLI
                     IDEAQNMTPNQVKGIITRAGQGTKIILLGDPQQIDKPFLDERTNGLSYAAKHMIDSSL
                     CWQITLSAEECERSALAMDAIKRL"
BASE COUNT         1232 a         1580 c         1189 g         1717 t
ORIGIN      
        1 ctccacggta ctgcggatcg tctctttgta tacaatattt ccggctccga attccaccca
       61 ttcgccgaaa cgctcgtgga tcagactctt taaaatctcg atctgcacct cacccatgac
      121 ctgcgcatgg atttccgcga ggcgctcatt ccagacaatg tggagctccg ggatttcttc
      181 ttctaactgg cgcagttttc caagcatctg atggacatcg cagtccggcg gaagctcgat
      241 gcggtacgtc agcaccggct ccagcaccgg tttttccgcc tcggtttcgt tgccgatgcc
      301 ctgaccggag aaagtgctgt tcagtcccgt tacggcacag atcgtccccg ccggtgcttc
      361 ctgtaccatc gtatagcccg caccggaata aatgcggatc tggtctgcct tttcttcgcc
      421 gagcatctgc tttaccttca gcacgccgcc cgtgattttc agatgcgtca gacggtttcc
      481 tgccgtgtcc cttgaaatct taaaaacgcg cgcgccaaaa tccccgccgt actccggcgt
      541 ttttgtaaac cggtccagaa ttttcagaaa ttccgtcacg ccctccatct tgagtgcaga
      601 gccaaagcag cacggaaaaa ccttccgctc tgcgatcatc gtgcggatct ggtcttcttc
      661 cacgcttccc gtttccagat actgctccag cacgtcctca tcacacatgg cgagttcttc
      721 cagcaattct gagttctgga gatcctgcga gaaattcagg cagtgctctg agagacggct
      781 ctgcagttct tccagcagtt tttccgcatc cgtgcccggc tgatccatct tgttgataaa
      841 gagaaagacc gggatctcat accgcgcgag cagccgccac agggtttcca catgaccctg
      901 cacgccgtcc gcaccgctga tcagaagaac cgcatagtcc atgacctgta aagtgcgctc
      961 catctccgcc gagaaatcca catggcccgg cgtgtcaagg agtgtgacct ccgtctcgtt
     1021 cagcttcagc acggcctgtt tggaaaagat cgtgataccc cttgcttttt ccagttcctc
     1081 cgtgtccaga aaggcgtcct tgtgatccac tcttccggct ttccggatct ggcctgtttt
     1141 atataaaatt ccctctgcca gcgtcgtctt tcccgcatcg acatgcgcta aaatgcctac
     1201 cgccagtttt ttggtttttt ccattttgtt tcatcctgtc tgtatgattc atatttttca
     1261 ggccccctca tcccctgcgg cccgactcac tgcttattgt accacaaaac ctctcattgc
     1321 ggaaccccgg agtgaaacgc aaaaaatctc aaagcggctt tgttcccccc gttaaatata
     1381 atattcaatc gcatcttccc gtttcatatg gaagatctca aaaatctgat ccctaattgc
     1441 cagaaaatcc tcactcgtac gatcccggtg ctttccaagc ggaatcgtaa gtactgcttt
     1501 gatctgccac ggattggcat cgaaaatcac cacgcggtcg gcgagataga tcgcctcctc
     1561 ggaaattgca aggtcctggt agctgatcca gtcaagcgaa gtttcgagtg ttgccgcatc
     1621 ctgtcccaga tactcctctg ccacccgaag cgcttctttt ttgtcgagct tttctcccgc
     1681 cgcaaaatag ccgtccagca gtttctgaaa cgatgcggga tctttttcga tcacatcggt
     1741 ccggaaatca gaccgcagca gagagaatcc tcccagagct cttcggagcg cgccagcacc
     1801 gttccaaagc cctgcnnnnn nnnnngaacg atattatcct tatctatctt ccaaagttca
     1861 aagccctgcg tcaccgcaat gtcgtccagc gtaagacctg ctttcgccag ctcaatcagt
     1921 tccgatgcac cgtcgatttt tccggcgtgc agtgcatctg tcagatccga ccaggagctg
     1981 agattgtcaa ttccattcgt tattgttttt tgctatcacg ggtgcaaatg taaaaccgga
     2041 gaacgcccgc ttttctgcct ggctgttctc cggttttgcc gtttcacaga ataaaatctc
     2101 tgtctgtttt tatgcctttg tctgcgcttt ttccgcattg ctgtacgcca tcgcgtaaaa
     2161 cagctcctgc gaattgagtt tcgtaccgtt cgattcccgg tccacatatt ttccgctggc
     2221 attcagacat tttccctttt cgttgtcctt catcatcgtc tcaaacatcc agtccagacg
     2281 ctcttttaag gtctcatcca gaaccggagc ggcaacctcg acgcggcgga tggtatttct
     2341 tgtcatgaaa tcggcggatg caagatagac cttctcgcgc tctttcgttc caaaccggta
     2401 aattctggaa tgctcaagga agcggccgac aatgctgaca accttgatgt tttccgtgta
     2461 tccctcgatt cccggaatca gacagcagat accgcggaca atcatttcga tcttcacgcc
     2521 cgcctgggaa gcctccacca gcttggtgat gattgcttta tctgtcaggg agttgatctt
     2581 gatgccgata tagctctctt cgccctgttt tgcatgtgca atttcttcgt ctatcatctc
     2641 aagcacccgg ttctgcagac atttcggtgc aaccagaaga aggtctgtct tttccacggt
     2701 ctcgcccttt aagagcgctg caaacacctc tgccgcctct tttccgnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtttccgg ttccgatctg
     2821 ggtcacatac gatacgccgt cctctgtttt tctggaaatc aggcacagct tggagtggac
     2881 tttaaagccg ttcaggccgt aaatcacgcg gcagccggcc tcctccagga tacgggaata
     2941 ctcgatattg ctctcctcat cgaagcgggc gcgcagctca acaaggacaa caacctcttt
     3001 tccattctct gctgcttcga ccagtgcatc aacaatctgg ctcttgtcag ccagacggta
     3061 cagtgtcatc ttaatcgaaa caacgctctc atcttttgcc gcctcgtcga gcaggctgat
     3121 aaacggtttg atgctctcaa atggatagga aagcagaaca tcttttttca taacctgcgg
     3181 aatcagcggc gctttcgaat ccagctgcgg tgtcatacgc ggggcgcggc gctgatagaa
     3241 cagttgctta gtgtcggccg ttccgttctg ctccagactg cgcagatatc cctgcagtgc
     3301 aaagacaaat gacagatcca gcggcactct ggacaggaat acatggtctt tgtccacctt
     3361 gatttcctta cagagcgatg aagtcagctt cttgttcagc tcacgggaca tctccatgcg
     3421 gaccgggctc atacgttttc tctgtttgat caggttttcc attgcatcac gataatccag
     3481 atcctcatcg taaatcgtct cggtatcgat atcggcattc cgcgttacac ggatcaggga
     3541 tttttctttt acaatataat ttttgaacat cttcggaagg aagtgcagaa tcagctcctc
     3601 ggagagcata aaggttccct ttctggtcgg gatgtcgatc agacggcgga atacgttgtt
     3661 gctgcatggg atgatcgcag tacgggtctt tccgcccttc gtctcaagga gtgcgaccgc
     3721 atagatatct ttgtttttca ggaacgggaa cggctgctgt ttgctcacaa tgttggcaga
     3781 cagatacggc gcgatctcat tatcaaaata cgtttccagc agttttccct cttcagcgga
     3841 cagcttattg aagttgatga ggcggattcc cttcggctcc aattctccca tcagctgctc
     3901 atagatcgcc gcttttttct gatccaggtc tcttgtctcc tgcagaatcg cttttacctg
     3961 ctgttcactc gtcatattcg ttttattttc ccggaccact tctttggatt ccatctgatc
     4021 catcaatgtg ccgacccgga cacggtagaa ctcatccagg tttgactggt agatcgaagc
     4081 aaacgtcaga cgctcggcaa gcgggacggc cgggtttccg gcttcgttta agactctttc
     4141 attgaatttc agccaggata attcacggtt catcatatag ggttctggtt ttaactgctg
     4201 ctttttcatg tattttaatt ctccttcttc tgcgcgttct ttcaaagatt ccgcttttct
     4261 tttcacttgc tgtaaaagcc ctgatggtta tcatataata agaaaatttt ttcatctatc
     4321 ctgctattgt tttgtttctg taaaatccat gtaaagccat ttttgttctt tttacaaaac
     4381 ttttcgtcca ttgaccgtca gtttaaacgt agttcccagt atttctgccg attttctgca
     4441 cagcatcatt cttccgagaa agatctcaaa atagtcatac atcgaacaga ttttttcatc
     4501 aatctggaga ttcaatgcga tctctgattt tttctcatcc actttcagct tggaatctgt
     4561 aaccgcataa tttactctgt catgaatatc gaaagcagat tgttcctttt gtcttacacg
     4621 gcttcttctg acatctgttt tatctgcgat gatcaaggca gctgagatca catcctccgg
     4681 acttcccgtt gattcatcgt ggtttccaat tgcagaaacg atggttactc tttcttccaa
     4741 cgtcatatcc gtttctttta aaagttcatt tgcaagaagt gcaccgtact ctgcatgatg
     4801 tgatctgtta atcacatttc caatatcatg catataacct gcaatttgag cgagttcaat
     4861 ctcatgttcc gaataaccca gctttttcag aatttttcct gcctgatggg caacctgcat
     4921 acagtgtttc tgcgaatgat ccgtatatcc cagctgtccc aggttttcgt ttcctttttt
     4981 cagatatgcg ttaatttctt tgttttttat aatttccttg tatgtcaatc gtttgttctc
     5041 ctctttttca gactacagtc ttttgattgc atccattgca agcgcagacc gttcacactc
     5101 ttctgcagac aaggtaatct gccagcagag agagctgtct atcatatgtt ttgccgcata
     5161 gctcaggcca ttcgttcgct catcgagaaa cggtttatca atctgctgcg gatcgccaag
     5221 cagaataatt ttcgttccct gtcccgctct tgtgatgatt cccttcacct gattgggtgt
     5281 catgttctgt gcttcatcga ttatcagata tgtccttgct atcgatcgtc cgcggataaa
     5341 attcatcgct tctgtctgaa tgattccccg ttcgaaaatc tccgtgattt tatcccccag
     5401 ttcctcttca tcctcatatc ttctcttctc atccgaatcc agaatctgct ccagattatc
     5461 aatcaccggc cgcatcagcg gtgcaatttt ttctttttca tcgcccggca gaaaaccaat
     5521 atcatcatca aactgtgcgt ttggccggca tacgatcaca cggcggtact ctcctgttgg
     5581 attattatag actttttcca ttccgaccgc gagcgaataa aatgtttttg cggttcctgc
     5641 cattcctttc agaataacaa gcggtgcttc ttctgcactt gtcataagtg cttcctgcat
     5701 aaaatactgc ccgacatttc tcggcgatac gccgtatggt ttgctctttt tataaaccag
     5761 cggaacaatt tttccgccag aca
//
LOCUS       DAOMUF010000134         5752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3807_length_5752_cov_4.558013, whole genome shotgun sequence.
ACCESSION   DAOMUF010000134 DAOMUF010000000
VERSION     DAOMUF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3807_length_5752_cov_4.558013"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLN77_07945"
     CDS             <1..780
                     /locus_tag="PLN77_07945"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRM44239.1"
                     /translation="EIEVVTNSRKDDHEIEIYKDLRFSDKSAEKIIAEVRGMFHTGRQ
                     RLNAVPTRQNEHADVLLSGDAVGSFFQYFAMHTNASYQYMGVAKTKIGEAITGADADP
                     LNIRLVPTLDGSAKNAPYDETGMPVAERVLFENGICRSYWGSLQHAHYIGMKDTTSMN
                     NMVVEGGSKTLDELRSMPHIEITDFSAFDMDAVSGTCGGEIRLAYESDGTTSHPTTSG
                     SLSARYDDVLKNLTFSKETQQINNRVVPCAVLLRDVTVAGE"
     gene            complement(1066..1152)
                     /locus_tag="PLN77_07950"
                     /pseudo
     CDS             complement(1066..1152)
                     /locus_tag="PLN77_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528792.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            complement(1145..3133)
                     /locus_tag="PLN77_07955"
     CDS             complement(1145..3133)
                     /locus_tag="PLN77_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRM44240.1"
                     /translation="MRQLEKEKQKKEHILVCLSSSPSNEKIIRTAAQMAQAFQAGFTA
                     LYVQTAEKTEKTPEDLARLQKHVRLAEQSGAEIVTTHSEDIALQIAEYARISEVTRIV
                     IGKSMMKQGYFHQRRVWTERLTDLVPDLEIHIIPDSDVYRRYQKRKKFPEWMELPSWK
                     ELMLTVLILAAATGIGEIFFRLGFTDANIITVYLFGVMLTSILTSGYTCSVVSSVASV
                     VLFNYFMTEPRLSLHAYGSGYPVTFAIMLGTSILTSTLASKLKENAKLSARDAFRTKI
                     LFNTSQLLQKAEDASEIFDITATQLIKLLGRNLVVAPVEKKKNGIVQGTLYNAETGIK
                     SEKVFNEKEQEILQWVLKNRKRAGAMTERFSEEPYLYLSIYAAQNRYGVIGIFIGQKP
                     LDVFENSILLSILGECAMALDREQSAREKEEAAVMAKNEQLRVNLLRTISHDLRTPLT
                     SILGNADSLISNFDALDEGMRKQIFSDIYEDAGWLIELVENLLALTKIEDGSVKLQLS
                     DQVVEDVVREALRHVERRKNEHKITVDCGEDVLLARMDAKLIVQVLVNLINNAVKYTQ
                     AGSKIRITAGKEEKNVWIAVEDNGPGIPAECREHVFEMFYSGKNKVSDSRRSLGLGLA
                     LCRSIVNAHGGELVLRDCAPHGCHFRFTLPLSEVNLNE"
     gene            complement(3369..4016)
                     /locus_tag="PLN77_07960"
     CDS             complement(3369..4016)
                     /locus_tag="PLN77_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRM44241.1"
                     /translation="MKNILLIGVGRFGRHIAMHLNQLGHQVMAVDRNEERIDAVMPYV
                     TNAQIGDSVNADFLRSLGVGNYDVCIVTIGSSFQDSLETACLLKELGAKWVVSRAERD
                     VQEKFLLRNGADQVVYPEKQMAKWTAIRYSSDHVFDYIEIDSQYAIFEVEIPENWIGK
                     NVGQLDIRKKYGINILATRKYGKTDVAVSPETVLDGDATILVLGEYKAIQKCFRI"
     gene            complement(4036..5376)
                     /locus_tag="PLN77_07965"
     CDS             complement(4036..5376)
                     /locus_tag="PLN77_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRM44242.1"
                     /translation="MWSIKLRYRKQWSSFEIIILGFAGVILAGALLLMLPVASREGNV
                     TPFTETLFTATSAACVTGLAVRDTGSYWSVFGQSVILLLIQIGGLGVVTIAVSIAMLS
                     GRKISLMQRSTMQDAISAPKVGGIVRLTRFILKGTFGIELLGALFLLPVFLRDYGGKG
                     IWMALFHSVSAFCNAGFDLLGKTDHLYPSLTSYVGNPLVNLTIMGLIVTGGIGFLTWD
                     DIYTKKYHFRQYCMQSKVILLTSLFLIFVPAAFFFFRDFAGMPVKERTLCALFQSVTT
                     RTAGFNTADLSGMTGASQAIMIVLMMIGGAPGSTAGGMKITTFAVLTANASATFRQRE
                     DTQMFGRRVGQEVLKNASTILMMYLFLVLMGGMVISVAENLPISECLFETASAVGTVG
                     LTLGITPRLGVLSKGILIALMFLGRVGGLTLVYAAMPGRKSAHAKMPLEKINVG"
BASE COUNT         1494 a         1540 c         1128 g         1590 t
ORIGIN      
        1 gagatcgagg ttgtcacaaa ctcccgcaag gatgatcatg agattgagat ctacaaggat
       61 ctccgcttct ccgacaaatc tgccgagaag atcatcgccg aagtgcgcgg aatgttccac
      121 accggccgcc agcgtctgaa tgctgttccg acccggcaga acgagcacgc cgatgttctc
      181 ttaagcggag acgccgtcgg cagcttcttc cagtattttg ctatgcatac caacgcttct
      241 tatcagtata tgggcgttgc aaaaacaaaa atcggcgaag ccatcaccgg cgcagatgct
      301 gatccgctga acatccgcct cgtgccgacg ctcgacggct ctgccaaaaa cgcgccgtac
      361 gacgaaaccg gtatgccggt agccgagcgc gttctgtttg aaaacggcat ctgccgctcc
      421 tactggggca gccttcagca tgcccattat atcggcatga aggatactac ttccatgaat
      481 aacatggtag ttgaaggcgg ttcgaaaaca ctcgacgagc tgcgcagcat gccgcatata
      541 gaaatcaccg atttcagtgc gtttgatatg gatgccgtaa gcggcacctg cggcggtgag
      601 atccgtctcg catatgagag cgacggaacc acctcccatc ctacaaccag cggatcactt
      661 tccgcccgtt atgacgatgt gctgaaaaat ctgactttct cgaaagagac acagcagata
      721 aacaaccgcg tggtaccgtg tgcggtactg ctgcgggatg tgacggtggc gggagaataa
      781 tttttcattc tgcagcatac gaaaaaggag catctccagg tttcaagttc gaaaccggag
      841 atgctccttt ttaattttct gtgtttatgt tcagtctttt cttcgtcttc tggatttctt
      901 tttgcttcca aaatccggaa caacgaccag ggtgttaaga cccagataag tttcaacatc
      961 ttctgccgtt ttgatattgt cattcagcac agtctgcagt gtcagaattg ccaaaacagg
     1021 aacaaaattt ctaaacagta atggggtctg accccaatgt ggttatttat aatcgtgtgt
     1081 tttcagtgtt gtcgtaatta ggttgtggac cggcgtatcg tcttccacga ccagtatcaa
     1141 tgtcttattc atttaaattc acctcactga gcggcagcgt aaaacgaaaa tgacagccgt
     1201 gcggagcgca gtcacgaagc accagttccc cgccatgcgc atttacaatg gagcggcaca
     1261 gcgccagccc aagaccaagg cttctgcggc tgtcactcac cttatttttt ccggaataga
     1321 acatttcaaa tacatgctcc ctgcactccg ccggaatgcc gggtccatta tcctccaccg
     1381 caatccatac gtttttttct tcttttccgg ctgtaatccg gatcttggat cctgcctgtg
     1441 tatattttac tgcattgttg atcaggttta ccaatacctg cacaatcagc tttgcatcca
     1501 tgcgggcgag cagtacatct tccccgcagt ccaccgtaat tttatgttcg tttttccgcc
     1561 gttctacatg gcgcagcgcc tcacgtacta catcctcaac cacctgatcg gataactgca
     1621 gttttacact tccatcttct attttcgtaa gcgcaagcag attttccacc agttcaatca
     1681 gccatccggc atcctcataa atatccgaaa aaatctgttt ccgcatccct tcgtccagtg
     1741 cgtcaaagtt actgatcaaa gagtctgcat tgccgagaat tgatgtcagt ggtgtgcgca
     1801 gatcatggga aatcgtccgc agcagattca cccgaagctg ctcatttttc gccatgactg
     1861 ccgcctcttc tttttctctc gcactttgtt cccgatccag tgccatggcg cactctccca
     1921 gaatagaaag caggatactg ttctcaaaca catccagtgg cttttgtccg atgaaaatgc
     1981 cgatcacacc atatctgttc tgtgccgcat agatggaaag gtacagatac ggttcttcag
     2041 aaaaacgttc tgtcatagct cccgcccgtt tccggttttt cagaacccac tgcaggattt
     2101 cctgttcttt ctcattaaaa actttttctg attttattcc tgtttcggcg ttataaagag
     2161 ttccctgcac aatgccgttt ttcttttttt ctaccggcgc cacaaccaga tttcttccca
     2221 gaagctttat cagctgcgtt gccgtaatat caaaaatttc tgacgcatcc tctgcctttt
     2281 gcagaagctg gctggtatta aaaagaatct tggttcgaaa ggcatctcgt gcagaaagct
     2341 ttgcattttc tttcaatttt gaagcaagcg tactggtcag gattgaagtg ccaagcatga
     2401 tcgcaaaggt caccggatag ccggaaccat acgcatgcaa cgaaagcctt ggctctgtca
     2461 taaaataatt aaacaggaca acacttgcca cagagctgac cacactacag gtataaccgc
     2521 tggtcagaat agatgtcagc atgacgccaa acagatatac cgtaataata ttggcatccg
     2581 taaaaccaag ccgaaaaaag atttctccga ttccggtcgc agcagccaga atcagaaccg
     2641 taagcatcag ttctttccag gaaggcagct ccatccactc cggaaatttt tttcgtttct
     2701 gatatctccg gtacacgtct gagtctggaa tgatgtgaat ttccagatca ggcacaagat
     2761 ccgtcagacg ctcggtccac acccttcgct gatggaaata gccctgcttc atcatgctct
     2821 ttccaatgac aatccgcgtc acctcggaaa ttcttgcgta ttctgcaatc tgcagggcaa
     2881 tatcctcact gtgtgtcgtt acaatttctg ccccgctctg ctctgccaga cgaacatgct
     2941 tctgcagcct tgccagatcc tccggtgttt tttctgtttt ttctgctgtc tgcacataca
     3001 atgccgtaaa gccggcctga aacgcctgtg ccatctgcgc cgcagtccga ataatttttt
     3061 cattggatgg cgaagaggac agacacacca ggatatgctc ttttttttgt ttttcttttt
     3121 ccaactgtct caccccgcct cctattgtag atcataaacg aagagaaagc tatagtcagg
     3181 aaatcttttt ttctcccgtt ccctgttctt gttttctgtt ttccgtgaaa ttttgtcccg
     3241 tttccggttc ctctcttctg ttttccgtga aaaatgtctc tatctgatct acgagcacgc
     3301 ctccagacaa aaaaccgacc agggtaacga agagcagtcc tacacttaac acagaaatca
     3361 cctctctttc agattcgaaa acatttctgg atcgctttat attcaccaag aaccaatatg
     3421 gtagcatcgc catccagaac tgtctccgga gacaccgcaa catctgtttt tccatatttt
     3481 cttgttgcca gaatattaat accgtatttt ttccggatat ccagctggcc gacatttttt
     3541 ccaatccagt tctccgggat ttcaacctca aaaatcgcat actgactgtc aatttcaata
     3601 tagtcaaaca catgatccga gctgtaacga atcgctgtcc attttgccat ctgtttttcc
     3661 ggataaacaa cctgatccgc tccgttccgc aacaggaatt tttcctgtac atcccgttct
     3721 gcccgggaca cgacccattt tgcgcccagc tccttcaaaa gacacgcggt ttccagcgaa
     3781 tcctgaaagc tgcttccgat cgtcacaata cagacatcat aatttcccac accaagggat
     3841 cgcagaaagt ctgcattgac actgtcaccg atctgtgcat ttgtcacata cggcatcacg
     3901 gcgtcgatcc gttcctcatt tcggtctacc gccatcacct gatgtccaag ctgattcaga
     3961 tgcatggcaa tatgtctccc aaatcttcct actccaatca gtaaaatatt tttcatctca
     4021 tctcttcctc tcttcttatc ctacatttat tttttccagc ggcatttttg catgtgcgct
     4081 ctttctcccc ggcatcgcag cataaacaag cgtcagaccg ccaacccttc caagaaacat
     4141 cagtgcgatc agaattcctt tcgaaagaac accgagccgc ggcgtaattc caagcgtcag
     4201 gccaaccgtt ccaacagcag atgctgtctc aaacagacac tcagaaatcg gcagattttc
     4261 tgccacactg atcaccattc ctcccattag caccagaaaa agatacatca tcagaatcgt
     4321 agatgcattt tttaatacct cctggcccac cctgcgtcca aacatctgtg tgtcctctct
     4381 ctgacggaac gtagcagacg cattagctgt cagcacggca aatgtggtaa ttttcattcc
     4441 gccagccgtt gatcccggtg ctccgccaat catcatcaga acaatcataa tcgcctggga
     4501 agcacctgtc atcccggaaa gatctgccgt attaaatccg gccgttcttg ttgtcacaga
     4561 ctgaaacaat gcgcagagcg tccgctcctt gaccggcatt ccggcgaaat cccggaagaa
     4621 aaagaaggcc gccggaacaa aaatcagaaa cagacttgta agcaggatca ccttgctttg
     4681 catgcagtac tgacgaaaat ggtatttttt tgtatagata tcatcccagg tcagaaatcc
     4741 gattccacct gttacaatta gccccataat cgtaagatta accagtggat ttcccacata
     4801 agaagtcagg gacggataca gatgatccgt ttttccaaga agatcaaatc ctgcattgca
     4861 gaatgcggat acagaatgaa acaacgccat ccagattcct tttccgccgt aatcccgtaa
     4921 aaaaacaggc agaagaaaca acgcccccaa cagttcaatt ccaaaggttc ctttgaggat
     4981 aaaacgcgtc aggcgcacaa ttccgcccac cttcggggca gaaatcgcat cctgcatcgt
     5041 gcttcgctgc atcagggaaa tttttctccc ggaaagcatc gcaatcgaaa cagcgatcgt
     5101 aaccacacca agaccaccga tctgaatgag cagcaaaatc acgctctgtc caaagacgga
     5161 ccagtagctt cctgtatctc tgactgccag tcctgtcaca caggcggctg atgttgcagt
     5221 aaacagtgtt tctgtaaatg gcgttacatt tccttcccgc gaagccacag gaagcatcaa
     5281 aagcagtgct cctgccagaa ttacccccgc aaatccaaga atgataattt caaacgacga
     5341 ccattgtttc ctgtatcgca gttttatact ccacatttct ctttttactc cttctttttg
     5401 attcttcctt attttagaga cttccgtttt aaaaacgtat aaataccttt tccatggcat
     5461 taagattgta taaagatttt tcgaatttca agcccaaaaa caggaaccgc ccaaaagttt
     5521 cccacaacag taatggggtc tgaccccaat gtgtgtatat ccttcttctt ttaccgtagt
     5581 aacgcattag ttggttaaac ttccgatttt tctccttttg aaagtagatt cttttgtcat
     5641 aaatatcaaa aaaagtattt acaaatcctt acgcatcagt tattatgtat ataatctatt
     5701 gagaagttcg tgtagaatta tgcaattttg tttttaataa atactctgtt tc
//
LOCUS       DAOMUF010000135         5696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3852_length_5696_cov_4.202092, whole genome shotgun sequence.
ACCESSION   DAOMUF010000135 DAOMUF010000000
VERSION     DAOMUF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3852_length_5696_cov_4.202092"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PLN77_07970"
     CDS             complement(<1..1131)
                     /locus_tag="PLN77_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44243.1"
                     /translation="MSLLEISHLTTKFETQQGTVSAVRDVSYHLEEGEVLGIVGESGS
                     GKSVGMLTLMGLLASNGRVEEGEILFDGENISPPHTKDRKEKRAYEKKMQQIRGNRIG
                     MIFQDPMTFLNPILKIGIQMTEGIRKHQNCSKKEAEAKAIELMRQVGIPSPEKRLDQY
                     PFEFSGGMRQRIIIATALACDPKLIIADEPTTALDVTVQAQILELLKKLTKEKGTSVI
                     MITHDLGVVASMCDRIAIMYAGQIVEEGTVDEIFYEPHHPYTKGLLNSINNSAKDNDE
                     PLVPIPGTPPDLLKLPKGCAFMSRCPYTMKICEVQASPVTTYSETHCCRCWLECMDET
                     KITVSGEEAALEDSMAGSHFYPDFAAVLLKQKVAEQYGLKAEN"
     gene            complement(1137..1517)
                     /locus_tag="PLN77_07975"
     CDS             complement(1137..1517)
                     /locus_tag="PLN77_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM44244.1"
                     /translation="MARQVRGQLIALKEQEFSMAALVIGASDARILFRHLLINAIGPI
                     IVQITFLVPNAIFTEAFLSFIGIGISAPMASWGSMAQDARQVLTMHPGQLLLPTICIC
                     ITIFSLNFLGEGIGDAFNPKKTRG"
     gene            complement(1502..1855)
                     /locus_tag="PLN77_07980"
     CDS             complement(1502..1855)
                     /locus_tag="PLN77_07980"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM44245.1"
                     /translation="MVRYIIKRLFIGVVTIWALITITFFLIRIMPGSPFEADNLSQSA
                     IEQLESTYGLDEPMWKQYILYMENLMHGDLGISYKKNVSVNTLIARGFPYTLSIGLLS
                     IAVSAFRAGSAWLVR"
     gene            complement(1952..2290)
                     /locus_tag="PLN77_07985"
     CDS             complement(1952..2290)
                     /locus_tag="PLN77_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRM44246.1"
                     /translation="MKKLTVIVKPANREDVIDIMESCAVYGVMETDITGYGNQKGYRV
                     MYRGTMSGPNVLMKCKFETVGDDKTIEVLKGFLEKKLCTGNIGDGKIFVEKVADVIRV
                     RTGQRGEEAL"
     gene            complement(2333..3547)
                     /locus_tag="PLN77_07990"
     CDS             complement(2333..3547)
                     /locus_tag="PLN77_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRM44247.1"
                     /translation="MENTQVLLNIVWTMVGAFLVYFMQAGFAMVETGFTRAKNSGNIL
                     MKNMMDFVLGSIFFFIIGFALMFGGSNAFIGTAGFFHPKSLADADGMFNGLPIGVFMI
                     FQTVFCATSATIVSGAMAERTKFISYLIYSAAISVFIYPITGHWIWGGGWLSQIGFHD
                     FAGSTAVHMVGGLCALAGAKMVGPRIGKYTKEGKPVAIPGHNLPLGALGVFILWFCWF
                     GFNCGSTTAATYNLGDIAMTTNLAAAAATLVTLIVTWVRYGKPDISMTMNGALAGLVA
                     ITASCDVVNDYEALFIGAVAGVVVVFAVEFFDKVVKIDDPVGAISVHGVCGALGTLFT
                     GIFGEGCSFITQLIGFVAVAAYTLILAFILFFVLKKTIGLRVTEKEEIDGLDMHEHGC
                     SAYANFHFHNDK"
     gene            complement(3635..3973)
                     /locus_tag="PLN77_07995"
     CDS             complement(3635..3973)
                     /locus_tag="PLN77_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRM44248.1"
                     /translation="MKKLEIVIRPESLENLELLLEEQKANGMMITNILGYGNQKGYRQ
                     MYRGAEIVAKMLPKVKVETVVEDDKVPKIVDFVVKNLSTGNYGDGKIFVYDVEDAVRI
                     RTGEHGEQAL"
     gene            complement(4223..5317)
                     /locus_tag="PLN77_08000"
     CDS             complement(4223..5317)
                     /locus_tag="PLN77_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44249.1"
                     /translation="MENTNVLFDRKRLVRLIVPLIVEQVLAVTVGMADMVMVSGAGET
                     AVSGISLVNTICVLQIVIFTSMASGGSVVGAQFLGSGDKKTACHAAEQLVMICGLIAQ
                     QEARRMKEENYEVVVFQGDPGTIDLTYEEIEAEWNKMLQTVPQIGKFRIIHQEKKRYT
                     FEDYSSQIGNADAALGIWVHKGVINEKLFEAHPNLKYIATLGHGFEDFDVEMTRRRGV
                     TITNTIYGDVTIAQYAMALLMNICHNVTVQSDYTKTGYWKEKETNPQASYNQLFVPQI
                     ELYQKTMGIIIDEEALLEALESRKVYMAGLDVVANEPPQYQIPLMQSPYTFITSHIAW
                     QPKAARLRAVDLAVKNFRSYLEGMPTSVIN"
BASE COUNT         1299 a         1516 c         1240 g         1641 t
ORIGIN      
        1 attttctgct tttaatccgt actgctccgc taccttctgt ttcagcagta ctgccgcaaa
       61 gtcaggataa aaatggcttc ccgccatgga gtcctccagt gccgcctcct ccccggaaac
      121 tgtgattttt gtttcatcca tacattccag ccagcatctg cagcagtgcg tctcgctgta
      181 ggttgtcacc ggagaggcct gtacctcaca gatcttcata gtgtaaggac agcggctcat
      241 aaatgcgcag cccttcggaa gctttaagag gtccggcggt gttcccggaa tcggaacgag
      301 cggctcgtcg ttatcctttg ccgagttgtt gatactgttc agaagacctt tcgtgtacgg
      361 atgatgcggt tcgtagaaaa tctcatcgac ggttccctct tctacgatct ggccggcgta
      421 cataatggca atccggtcgc acatagaagc gacaacgccg aggtcgtggg taatcatgat
      481 aacagaggtt cctttttcct tcgtcagctt tttcaaaagc tccagaatct gtgcctgtac
      541 ggtcacatcc agtgcggttg tcggctcatc tgcgataatc agcttcggat cacacgccag
      601 agccgtcgca atgatgattc tctgcctcat accgccggaa aattcgaacg gatactgatc
      661 gagccgtttt tccggacttg gaattccgac ctgccgcatc agctcaatgg ctttcgcttc
      721 cgcctctttt ttggagcaat tctgatgctt tcgaattccc tctgtcatct gaattccgat
      781 tttcaggatc ggattcagaa atgtcatcgg atcctggaaa atcatgccga tgcggtttcc
      841 gcggatctgc tgcattttct tttcataagc ccgtttttct ttccggtcct tcgtgtgcgg
      901 cggagaaatg ttttctccgt cgaaaaggat ctctccttct tccactctgc cgttcgatgc
      961 cagaagcccc atcagggtca gcatgccgac actttttccg gaaccggact cgccgacaat
     1021 accaagtacc tcgccctctt ccagatggta ggaaacatcg cggactgcgc tgacggttcc
     1081 ctgctgcgtt tcaaattttg ttgtgagatg gctgatttcg agtaaactca tacgcttcac
     1141 cccctggttt tctttggatt aaaggcatcg ccgattccct cgccgaggaa gttcagggag
     1201 aaaatggtga tacagatgca gatggtcggc aaaagcagct gtcccgggtg catggtaagc
     1261 acctgacggg catcctgtgc catggaaccc cagcttgcca tcggagcgga aatgccgatt
     1321 ccaatgaagc ttaagaaggc ttcagtaaaa atggcattcg gaacaaggaa ggtaatctgt
     1381 acaatgatcg gtccgatggc attgatcaaa aggtgacgga agagaattct cgcatcactt
     1441 gctccgatga cgagtgctgc catggaaaat tcctgctcct ttaaggcgat cagctggcct
     1501 cttacctgac gagccatgcc gatccagccc ggaatgcaga taccgcaata gagagaagtc
     1561 cgatgcttag cgtgtacgga aatcctcttg cgatcagtgt attaacagaa acgttctttt
     1621 tataggaaat gccgagatct ccgtgcatca gattttccat gtaaagaata tactgtttcc
     1681 acatgggttc atccagtccg taggtcgatt cgagctgttc aatcgcgctc tgggaaagat
     1741 tatccgcctc gaacgggctg cccggcataa tccgaatcag gaaaaaggta atggtgatca
     1801 gtgcccagat cgtcaccact ccaatgaata aacgttttat gatgtatctt accatgcagc
     1861 attcctcccg ttccggcttt tttagctcag aagaggcagc cgaacgatct gttctggctg
     1921 cctctttgca cctttttact gtcttttgtt tttataatgc ttcctcgccg cgctggccgg
     1981 tacgaacccg gatgacgtct gccactttct cgacaaaaat ctttccgtct ccgatgtttc
     2041 cggtacacag cttcttttcc agaaagcctt ttaatacttc aattgtctta tcgtcaccga
     2101 cggtctcgaa tttgcatttc atcagtacat tcggtccgct catggttcca cggtacatca
     2161 cccggtaacc cttctggttt ccatatccgg tgatatccgt ttccatcacg ccgtacacgg
     2221 cacacgattc catgatgtcg atgacatcct cccggttcgc gggtttgaca ataactgtca
     2281 gctttttcat atccgcacca cctttttatt gatccttttt catcgattct ttttatttgt
     2341 cattgtggaa gtggaagttt gcataagcgc tgcatccgtg ctcgtgcata tccagtccgt
     2401 cgatctcttc cttctcggtt acgcgaagtc cgatggtctt tttcaggacg aagaacagaa
     2461 tgaacgccag gatcagggta tatgctgcaa ctgctacaaa accgatcagc tgggtgataa
     2521 agctgcatcc ctcgccgaaa ataccggtaa ataaggttcc gagtgctcca cagacaccgt
     2581 gtacactgat tgcaccaacc gggtcatcga tttttacgac tttatcaaag aattcaactg
     2641 cgaatacaac gacaacacct gcaactgctc caatgaacag tgcctcgtaa tcattgacta
     2701 catcacagct tgctgtaata gctaccagtc cggccagtgc gccgttcatt gtcatactga
     2761 tatccggttt tccataacgt acccaggtaa caatcagggt aacaagtgtt gctgctgctg
     2821 ccgcaaggtt tgtagtcatg gcaatgtcgc caagattata ggttgctgct gtggtagatc
     2881 cacagttaaa tccgaaccag cagaaccaga ggataaagac accaagtgct ccaagcggaa
     2941 ggttatgacc aggaatagca accggttttc cctcttttgt gtattttccg atacgaggtc
     3001 cgaccatttt tgcaccggca agggcacaca gaccgccgac catatgaact gcggtagatc
     3061 ctgcaaaatc atggaacccg atctggctta accagccgcc tccccagatc cagtgtccgg
     3121 tgatcggata aatgaaaacg ctgatcgctg cggagtaaat cagataagag ataaatttgg
     3181 ttctctctgc catagctcct gatacaatcg ttgccgatgt tgcacagaac acggtctgga
     3241 aaatcatgaa tacgccgatc ggcagtccgt tgaacatgcc gtctgcatct gccagcgatt
     3301 tcggatgaaa gaaacctgct gttccgataa aggcattgct tcctccgaac atcagcgcaa
     3361 atccgatgat aaagaagaaa atagagccga gcacgaaatc catcatattc ttcatcagaa
     3421 tgtttccgga gtttttcgct cttgtaaatc ctgtctccac catggcaaat ccggcctgca
     3481 taaaatacac cagaaaggct ccgaccatgg tccatacgat attgagtaat acctgtgtgt
     3541 tttccataat aagttcctcc tcattcccat cttacctgtt cccgtctgca gatcttctga
     3601 aacggtcatc ctgaaacggt agctttcaga ttttttaaag tgcctgttcg ccgtgctcac
     3661 ctgtccggat ccgcactgca tcttcgacat cgtaaacgaa aatttttcca tcgccgtagt
     3721 ttccggttga caggtttttc acaacaaaat caacgatttt cggaaccttg tcgtcctcca
     3781 caacggtctc cactttaacc ttcggaagca ttttggccac gatttccgct ccacggtaca
     3841 tctggcgata tcccttctgg tttccatatc caaggatatt tgtgatcatc ataccgtttg
     3901 ccttctgctc ctcgaggagt agctccaggt tttcgaggct ctccggacga attacaattt
     3961 ccagcttttt catgattttc cctccctttc cgaaccggct cctgtacagc ttctgatttt
     4021 tccggctgcg gatttctttt ttatccgcct tcccggtttt ctcttccatt catttaccgg
     4081 ctctgctgct tctatataga aatctgtttt ctgacagatt ttctaacctc ttctttcatg
     4141 cctggactgt tccgcagctc tgctgttttt acagttgttt ttacagtcgt ttttttgcag
     4201 ctgttttcac aatccgctgt ttttaattaa tcacactcgt cggcattccc tccaggtaag
     4261 accggaagtt tttcaccgcc agatctactg cgcggagtct tgctgccttc ggctgccagg
     4321 cgatatggga agtaataaag gtgtacggcg actgcatcag cggaatctga tactgcggcg
     4381 gctcgtttgc aacaacatcg agtcctgcca tataaacctt tctcgattcc agcgcctcaa
     4441 gaagcgcctc ctcatcgatg atgattccca tcgttttctg gtagagctca atctgcggca
     4501 cgaacagttg attgtagctt gcctgcggat tcgtttcttt ttctttccag taccctgttt
     4561 tggtgtaatc gctctgtacc gtcacgttat gacagatgtt catgagcagt gccatcgcat
     4621 actgggcaat ggtgacatcg ccgtaaatcg tattcgttat ggtcacgccc cggcgtcttg
     4681 tcatttccac atcgaagtct tcaaatccat gtcccagcgt tgcgatatat ttcagattcg
     4741 gatgcgcttc aaagagcttt tcgttgatca ctcctttgtg gacccagata ccaagtgctg
     4801 cgtcggcgtt cccgatctga ctgctgtaat cttcgaaggt gtacctcttt ttctcctgat
     4861 ggatgatacg gaactttccg atctgtggta cggtctgcag cattttgttc cactctgctt
     4921 ctatttcttc gtacgtcaga tcgatggttc ccgggtctcc ctgaaaaacg actacctcat
     4981 agttttcttc tttcatacgc ctcgcctcct gttgtgcaat cagtccgcag atcataacga
     5041 gctgttcggc tgcgtgacac gcggttttct tatccccgct tccgaggaac tgtgctccga
     5101 caacggaacc gccgcttgcc atcgatgtga aaatcacaat ctgcagtacg cagatcgtat
     5161 tgacaagtga gattccggaa acagccgttt caccggctcc ggataccatc accatgtctg
     5221 ccataccgac ggtaacggcc agcacctgtt ccacaatcaa gggaacgatc agccggacca
     5281 gacgctttcg gtcaaataag acgtttgtgt tttccatcgg actttctcct ttccggtaaa
     5341 atcaaaaggc gacctggaaa tcttccttcc agatcgcccc gttgcgaact ctttactgac
     5401 gtttctcctt tggattgaaa aacgcggcac gaagctttcc cttcgtaccg cgccttcgcg
     5461 aatccattac ttatatggag tttatcttga aaaacagaca gagcgttttc cggttttctg
     5521 agagtgtttt ttgggagaaa taaaaccttg gagaaaggga gaaaattaca aacggagcga
     5581 ttctattccc agaaccgctc cgttgcgtta tctttactct attctttttc tctgctgttt
     5641 ttcaacatag gcacattcta gcggaattcc cgctttttgt caatccttaa attttt
//
LOCUS       DAOMUF010000136         5675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3878_length_5675_cov_4.898399, whole genome shotgun sequence.
ACCESSION   DAOMUF010000136 DAOMUF010000000
VERSION     DAOMUF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3878_length_5675_cov_4.898399"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLN77_08005"
     CDS             <1..345
                     /locus_tag="PLN77_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HRM44250.1"
                     /translation="LIPEQTAMYHKYHELIREGEYYRILSYRENHRSDCWAVASEDKN
                     EVLVTYVQVLAQVNMPSRKVRLRGFDPAKKYCLEGTDEVYSGEMLMNAGFRMKDFWGD
                     FVSRLYHFVAVD"
     gene            358..1359
                     /locus_tag="PLN77_08010"
     CDS             358..1359
                     /locus_tag="PLN77_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRM44251.1"
                     /translation="MAKVRLADIAKVAGVSAVTVHNAITGQKGVSDEMRARILEIAKE
                     MGYRQPGSGARNTQGKSLRCIGVVIPERYLAEYTTFYWKMYQEMALAATEKSCMVTVE
                     ILKQEMEDEMIVPCIAKEQTVDALILMGDTSRDYIHYLKKNVDLPIVFLDFYDNELAK
                     DAVIADNFHGMYLMTEYLYEHGLTKLAFVGSIHFSSSIMDRYCGFSKAVLEHRDVTPA
                     EWILEDRDERGQIEIHLPEQMPEAFVCNCDLTAGLLMLELEKRGISVPEDVSVVGFDN
                     YLYPGFPDLKITSYEINTKAMVKVALEKALKQIRGAASGKNMEIVSGQLVPKESVKL"
     gene            1665..2687
                     /locus_tag="PLN77_08015"
     CDS             1665..2687
                     /locus_tag="PLN77_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRM44252.1"
                     /translation="MSEVKIIGDAVKNMPWQEGPAEFSGAPVWRYSENPIIGRNPVKE
                     VARIFNSAVMPYGDEFIGVFRGEQTNGIPYIYLGRSKDAIHWNFDENKIQFVDEEGKP
                     FMPVYAYDPRLVKVEDTYYIIWCQDFYGAAIGMAKTKDFETFVRVENPFLPFNRNAVL
                     FPRKINGNFVMLSRPSDSGHTPFGDIFLSESPDMLYWGKHRHVMSKSPEWWESLKIGG
                     GAAPIETNEGWLLFYHGVSGTCNGYVYSIGGAILDKENPSIVKYRCQNFLLTPEEWYE
                     ERGFVPNVTFPCATIHDAETGKIAIYYGAADTYVGLAFTTVDLIVDYIKKNSVVRESD
                     TEIGRR"
     gene            2706..3884
                     /locus_tag="PLN77_08020"
     CDS             2706..3884
                     /locus_tag="PLN77_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRM44253.1"
                     /translation="MQSLAKEVKQELTGRIVPFWEGLRDEEYGGFYGYQDFDLNVQKT
                     YEKGCILNSRILWFFSNAYLTLKDEKLRADAEHAYRFMKQACVDREYGGVFWSVTYDG
                     KPLDTTKHTYNQAFAIYALSTYFDATGDTEALELAKSLQQIIETHCTDEYGYLEAFTR
                     DFKPESNEKLSENGVMAEKTMNTLLHVFEAYTELYRVTKDPFTGDRLRFMMDLFADKV
                     YNKEKGRQEVFFDRTWNTLIDLYSYGHDIETSWLIDRGTEVLGDPAYREKLLPITGEI
                     AENIYNTAYKNHSLMNECERGVDDTTRVWWVQAEAVVGFLNAWQKKPEETKYLDAAKD
                     IWGYIKEYVIDKREGSEWFWCVNADGSPIHEPIVEPWKCPYHNGRMCMEVIGRLKDAE
                     "
     gene            3874..5049
                     /locus_tag="PLN77_08025"
     CDS             3874..5049
                     /locus_tag="PLN77_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRM44254.1"
                     /translation="MLNKQYYVEKAKVDRLVARKNTKADFYNGIYDRYEYPVLTREFA
                     PVHWRYDLDEETNPYFMERLGINAVMNSGAIYLNGKYYLVVRVEGNDRKSFFAVAESD
                     TGIDGFHFWDYPVVLPDTCAEETNVYDMRLTQHEDGWIYGVFCSESKDKNDPDLSAAV
                     AEAGIVRTKDLKTWERLDNLKTLRSPQQRNVVLHPEFVDGKYAFYTRPMDGFIETGSG
                     GGIGFGLCDDITHAVIDEEKIISKRIYHTLTESKNGAGAVPIRGKKCWINIAHGVRNT
                     AAGLRYVLYVFGTDLNDPSKVIAEPSGVFLVPLGKERVGDVSNVVFTNGAIARENGEV
                     YIYYASCDTRMHVATTTIDKLEDYLFNTPQDPHRSPDCVKQRCELISKNLELLKNEK"
     gene            complement(5278..>5675)
                     /locus_tag="PLN77_08030"
     CDS             complement(5278..>5675)
                     /locus_tag="PLN77_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM44255.1"
                     /translation="EQSLQTLSEKRVLLAEDNDLNAEIVLTVLEENGIHAEHVKDGAL
                     CLKAVQNAPEDYYNVILMDIQMPNMNGYQAAEAIRALPGKRGEIPIVAMTANAFEEDR
                     RKALESGMNAHIAKPVDVSVLLETLSRFS"
BASE COUNT         1579 a         1258 c         1532 g         1306 t
ORIGIN      
        1 ctgattccgg agcagaccgc gatgtatcac aaatatcatg aactgatccg cgagggagag
       61 tattatcgta ttctttccta ccgtgaaaac cacagatccg actgctgggc agtagcttct
      121 gaggataaaa acgaagtgct tgtaacttac gtacaggtgc tggcacaggt aaatatgccg
      181 tcccgcaagg tacgcctgcg cggctttgat ccggcgaaaa aatattgtct ggagggaacc
      241 gatgaggttt acagcggcga gatgctgatg aatgccggtt tccggatgaa agatttctgg
      301 ggtgatttcg tcagcagact gtatcacttt gttgcagtag actaaggagc aacggatatg
      361 gcaaaagtaa gattggcaga tattgcaaag gtggcgggcg tcagcgccgt caccgtgcat
      421 aacgcgatta caggacagaa aggggtcagc gatgagatgc gcgcccggat tctggaaatt
      481 gcaaaggaaa tggggtaccg ccagccggga agcggagcga gaaatacaca gggaaaaagc
      541 ctgcgctgca tcggagtggt gattccggaa cggtatctgg cagagtatac gacattttac
      601 tggaaaatgt atcaggagat ggcactggcg gcgacggaaa agagctgtat ggtgaccgtg
      661 gagatcctga aacaggagat ggaagatgag atgatcgtgc cgtgcatcgc caaggagcag
      721 accgtcgatg cgctgatcct gatgggagac acgagccgcg attacatcca ctatctgaag
      781 aaaaacgtgg atcttccgat cgtatttctg gatttctacg acaatgagct ggcaaaagat
      841 gccgtgatcg cagataactt tcacggaatg tatctgatga cggaatacct gtatgagcat
      901 ggcctgacca agctggcttt tgtcggttcg attcattttt ccagcagtat catggatcgg
      961 tactgcggtt tttccaaggc tgttctggaa caccgcgatg tgacaccggc ggaatggatt
     1021 ctggaggacc gtgatgagcg gggccagatc gaaattcatc tcccggaaca gatgccggaa
     1081 gcgttcgtct gcaactgcga cctgaccgcc ggccttctga tgctcgagct ggaaaaacgc
     1141 ggcatctccg tgccggagga cgtttccgtt gtcggcttcg acaattacct gtatccgggt
     1201 ttcccggatc tcaagatcac ttcgtatgag atcaatacga aggcgatggt aaaagtagcg
     1261 ctcgaaaaag cgctgaagca gatccgcggc gcggcatccg gcaaaaatat ggaaattgtc
     1321 tccggacagc tggtgccgaa agagagcgta aagctttgat ttttataact gaattataaa
     1381 ttaagaaacg gggcattttg aaatgccccg tttttgtaca tattacacaa caaagtcact
     1441 taaaaattga cagtatctaa cggaacttat tttttaagta atttccagtt gaaaaattaa
     1501 ccatctatgt taaaattaag ctaaaggact gggcgtgagg gtgataaaac acggcggttc
     1561 ggagaaaaaa gaattaaaaa acaagattct gtccactaac agaaacttaa aaaaacaaag
     1621 tacaactcat aaacaaacaa tatttttcat ggaggtttca aaaaatgagc gaagttaaaa
     1681 ttattggaga tgctgtcaaa aatatgccat ggcaggaagg cccggctgag ttttccggtg
     1741 caccggtatg gagatacagc gagaatccga tcatcggaag aaatccggtg aaggaagtag
     1801 caagaatctt caacagcgcc gttatgccgt acggcgacga gttcatcgga gtattccgtg
     1861 gtgagcagac aaacggaatc ccgtacatct accttggaag aagcaaagat gcgatccact
     1921 ggaatttcga tgagaacaaa atccagttcg ttgacgaaga aggcaaaccg ttcatgccgg
     1981 tttacgcata tgacccgcgt cttgtaaaag tagaagatac ctactacatc atctggtgcc
     2041 aggacttcta cggtgcagcc atcggaatgg caaaaacaaa agattttgaa accttcgtac
     2101 gtgtggagaa tccattcctt ccattcaaca gaaatgccgt tctcttcccg agaaaaatca
     2161 acggcaactt cgtgatgctg tcccgtccaa gcgacagcgg ccacacaccg ttcggtgaca
     2221 tcttcctgtc cgagagcccg gatatgctgt actggggcaa acaccgccac gtcatgagca
     2281 agagcccgga gtggtgggag tccttaaaga tcggcggcgg cgcagctccg attgagacca
     2341 acgaaggatg gctgctgttc taccacggcg taagcggcac ctgcaatggc tatgtataca
     2401 gcatcggcgg cgcaatcctc gacaaagaga atccgtccat cgtaaaatac cgctgccaga
     2461 acttccttct gacaccggaa gagtggtatg aggagagagg cttcgttccg aacgtaacct
     2521 tcccgtgcgc aaccattcac gatgcagaga ccggaaagat cgcgatctac tacggcgcag
     2581 ccgatacgta tgtaggactg gcattcacaa ccgtagatct gattgtagat tatatcaaga
     2641 aaaacagtgt cgtacgcgag tctgacaccg aaatcggaag aagataagaa agaaggaaag
     2701 aaaagatgca gtctttggca aaggaagtaa agcaggagct tactggacgg atcgttccgt
     2761 tctgggaggg cttaagagat gaggaatacg gcggatttta cggctatcag gactttgatc
     2821 tgaacgtgca gaaaacgtat gagaagggct gcatcttaaa cagcagaatt ctctggtttt
     2881 tctcgaatgc gtacctgacc ttaaaggacg agaagctgcg cgccgatgcg gagcatgcgt
     2941 accgctttat gaaacaggcg tgtgtggacc gcgagtacgg tggcgtgttc tggtctgtca
     3001 cctacgacgg aaagccgctc gatacgacaa agcatacgta caatcaggca tttgcaattt
     3061 atgcgctgtc tacgtatttt gatgcgacgg gcgacaccga ggcgctggag ctggcaaaat
     3121 cgctgcagca gatcatcgag acacactgca ccgacgagta cggttatctc gaggcattta
     3181 cccgcgattt caagccggag agcaatgaaa agctctcgga gaacggcgtc atggcagaga
     3241 agaccatgaa tacgctgctg catgttttcg aagcatatac cgagctgtac cgcgtgacaa
     3301 aggatccgtt taccggtgac cgtctccgct ttatgatgga tctcttcgcg gataaagtat
     3361 acaacaagga aaaaggccgt caggaagtat ttttcgaccg gacatggaat acgctgattg
     3421 atctgtattc ttatggccat gatatcgaga cttcctggct gatcgaccgg ggtacagaag
     3481 tgctcggcga tccggcttac cgggaaaaac tgctgccgat caccggagag attgcggaaa
     3541 atatctacaa cacggcatac aaaaatcatt cgctgatgaa tgagtgcgag cgcggtgtgg
     3601 atgataccac aagagtctgg tgggtacagg cagaagctgt cgttggattt ttaaatgcat
     3661 ggcagaaaaa accagaggaa acaaagtatc tcgatgcggc aaaagacatc tggggctaca
     3721 tcaaggaata tgtgatcgac aaacgagagg ggtctgagtg gttctggtgc gtgaatgcag
     3781 acggaagccc gattcatgag ccgattgtag aaccgtggaa atgcccgtat cacaatggac
     3841 ggatgtgtat ggaagtaatt gggaggttaa aagatgctga ataaacagta ttatgtggaa
     3901 aaagccaagg tagaccgcct tgtggcaaga aagaacacaa aagcagattt ttacaacggt
     3961 atttatgacc gctatgagta tccggtgctg acaagagagt tcgctcctgt tcactggaga
     4021 tatgatctgg atgaggagac gaatccgtac tttatggagc gtctcggcat caacgctgtg
     4081 atgaactccg gcgcgattta tctgaatgga aagtattatc tcgttgtccg cgtggagggc
     4141 aatgacagaa aatctttctt tgctgttgca gagagtgata ctggtatcga cggcttccat
     4201 ttctgggatt acccggttgt gctgccggat acctgcgcag aggaaaccaa cgtttatgac
     4261 atgcgtctga cccagcacga ggacggctgg atttacggag tgttctgctc tgagagcaag
     4321 gacaaaaacg atccggatct gtccgcggct gttgctgagg ctggtatcgt aagaacgaaa
     4381 gacctgaaga cctgggagcg tctggacaac ctgaaaacct tacgttctcc gcagcagaga
     4441 aacgttgttc tgcatccgga gttcgtagac ggaaaatatg ccttctacac ccgtccgatg
     4501 gatggtttca ttgagaccgg aagcggcgga ggaatcggct tcggactctg cgatgatatc
     4561 acacacgctg tcatcgacga ggagaagatc atcagcaaac gtatttatca taccctgacc
     4621 gagtccaaaa acggtgccgg agcagtgccg atccgcggta agaaatgctg gatcaacatt
     4681 gcacacggtg taagaaatac agcagcaggc cttcgctacg ttctgtatgt cttcggtacc
     4741 gatctgaacg atccgtccaa agtcattgca gagccgtccg gtgtattcct ggttccgctt
     4801 ggaaaagaga gagtcggaga cgtttccaac gttgtattta caaacggcgc gattgcgcgt
     4861 gagaacggag aggtttacat ctactatgca tcctgcgata caagaatgca tgtagcaacc
     4921 accacaatcg acaaactcga ggactatctg ttcaacacac cgcaggatcc gcacagatcc
     4981 ccggactgcg taaaacagag atgcgagctg atctccaaaa acctggagct gctgaaaaac
     5041 gagaaataaa agcgtccgct gcggcgcatg aaaaaaggag ctgttctctt tacgggaacg
     5101 ctcctttttt catgcatatt atttgttttt gggtttctga tcgtcgatgg aagttaaaat
     5161 tccatagaag aggcgcggtg catcgggatc tgtaacggga ccgtattttc tgcggaagaa
     5221 tgctccgcta ctttcatcat tttatctgta ttcatatgca ttccagatct gtaaaggtca
     5281 tgaaaacctg gaaagtgttt cgagaagaac cgaaacgtca acgggtttcg cgatatgggc
     5341 gttcatgccg gattccagtg cttttcggcg gtcctcttca aacgcgtttg ccgtcatggc
     5401 gacaatcggg atttccccgc gttttcccgg gagtgcacgg atcgcttctg cggcctgata
     5461 gccgttcatg ttcggcatct gaatatccat cagaataaca ttgtaataat cttctggtgc
     5521 gttctggact gcttttaagc agagggcgcc gtcttttacg tgctcggcgt gaatgccgtt
     5581 ttcttccaga acggtgagta caatttctgc attcaggtca ttatcctcgg caagcagcac
     5641 gcgtttttcg gaaagcgtct gcagggactg ctcga
//
LOCUS       DAOMUF010000137         5641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3927_length_5641_cov_3.170247, whole genome shotgun sequence.
ACCESSION   DAOMUF010000137 DAOMUF010000000
VERSION     DAOMUF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3927_length_5641_cov_3.170247"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1832)
                     /locus_tag="PLN77_08035"
     CDS             complement(<1..1832)
                     /locus_tag="PLN77_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRM44256.1"
                     /translation="MKPYLKLETHPLADPKNVVKGDKYRITVLTEHLLRLEYSEDGEF
                     IDAASQTVVNRDFPAVEFSVKDTGDELELRTKYLQLNYNKKAFSANGLSCKTTAVGIS
                     SVPAWHYGDPTQDLGGTARTLDQVNGACELEPGIMSWFGSAVLDDSKSLLMTEDGWVT
                     PRKKGVQDLYFFGYGWNFRLALKDFYHLCGKTPMLPRFALGNWWSRYWRYSEASYMKL
                     MEDFDKENVPFSVAVIDMDWHRVDDVDPKYGSGWTGYSWNKKLFPDPERFLGKLHARG
                     MKTTLNVHPADGIRAYEDCYPEVAEAMGVDAKKEEPVNFDIADPKFVDVYFEKVHHPM
                     EKEGVDFWWIDWQQGQSTKIEGLDPLWMLNHYHFLDSGRDGKRPMTFSRYAGPGSHRY
                     PVGFSGDTHITWESLDFQPYFTSTASNIGYGWWSHDIGGHMLGYRDDELEARWYQYGV
                     FSPINRLHSSNSAFSGKEPWNFSMEVRSTMDDFLRLRHKLVPYLYTMNYRAWKEDTPI
                     VVPMYYDYPETVFWGKNQAPNQYFFGSELMVTPVTTKCIPELKRAKTKVWLPEGTWFD
                     FFNGMIYEGGRSMDLYRTLEEIPVLAKAGAIIPMTEKISALDVES"
     gene            complement(1933..3603)
                     /locus_tag="PLN77_08040"
     CDS             complement(1933..3603)
                     /locus_tag="PLN77_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRM44257.1"
                     /translation="MDKKWWKEAVVYQIYPRSFMDSNGDGIGDLNGITSRLDYLKDLG
                     VDVIWLSPVYQSPNDDNGYDISDYRAIMQEFGTMEDFDRMLSEMHKRGIRLVMDLVVN
                     HTSDEHKWFVESRKSKDNPYRDYYIWRDAKEGKEPNNWGSCFSGSAWKYDPQTEMYYL
                     HLFSTKQPDLNWENPAVRKEVFDMMNWWCEKGIDGFRMDVISMISKPEGLPDGKIPEG
                     GLYGDSGCVNGPHVHEYLQEMNREVLSKYDLMTVGECAGVTIEEAEKYASADGKELSM
                     VFQFELVDLASGTYGKWTDKRVPLKDFKRVMTAWQTELEGKAWNSLFLGNHDQPRCLS
                     RFGDDSPEYRVVSAKMLATCLHMMQGTPYVYQGDELGMKNVPFHDLSEFRDIESINAF
                     HELTGAGKVAPDDMMRYLNLRGRDNARTPMQWDDTASAGFTTGTPWIKVGPDYETVNA
                     KNEMADPDSVYNYYKKLIHLRKEKDIMVYGTYELLDPEDEALYVYTRTLGDQKLLVMC
                     NFTKEEQEYTVPETFAGAEVLISNYPQSGAAAGARKLRAYEAVVLETK"
     gene            complement(3758..4774)
                     /locus_tag="PLN77_08045"
     CDS             complement(3758..4774)
                     /locus_tag="PLN77_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM44258.1"
                     /translation="MIRIKDIADRAGVSPTTVSNVIHGKTGRVSKATVEKINRILKEM
                     EYVPSISARMLANNSSGLIGVALGFMKKGNASSFEDPFVSAMLGNLEYQIREHGYYMM
                     LVARHEQDDIMQQALGWNFDGMIAMALKEKEIAELSERLGKPLVTIDQYLPPELGVRS
                     ITMDDRGGAYQMSQYLIGKGHKKFLFLSDCDLGVDHYRWLGVRQAMEEAGIEDFESRH
                     IVIPWNPEQREKAYEEMLPFFKKQTALFFSSDYYALEASNFLQNRGIKVPEEISIAGF
                     DDVTYATLARPKLTTVHQMVDGKARRAVEVLMHLIQDEPVQKDIPPLPTTLVERESVR
                     DLTK"
     gene            5124..>5641
                     /locus_tag="PLN77_08050"
     CDS             5124..>5641
                     /locus_tag="PLN77_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44259.1"
                     /translation="MLCQAGFASSTPFTGASWELVGEYPILGSNGAVQSVCATEDYII
                     CIENFNDLTTEPDVVSAYYKNDTDADGNPVTQYSLAHQVRDADFAHANGMAYNPVTHE
                     ILVSGYSSPDASNYGCIFRLDPDTLEQKERIQLSTSYNILGIDYLPSTGGYLIQTDAD
                     GGYGFKLLDVSL"
BASE COUNT         1273 a         1562 c         1221 g         1585 t
ORIGIN      
        1 gattctacat ccagcgcact gatcttctct gtcatcggga taatcgcacc tgctttcgca
       61 agaaccggga tttcctccag cgtacggtac agatccatac ttctgccgcc ctcataaatc
      121 attccgttga agaaatcaaa ccaggttccc tccggcagcc ataccttcgt cttggcacgc
      181 ttcagctccg gaatgcattt cgttgtcacc ggtgttacca tcagctcgct tccgaagaag
      241 tactgattcg gtgcctgatt tttgccccag aaaaccgtct ccgggtaatc ataatacatc
      301 ggaacaacga tcggtgtgtc ttctttccat gcacggtagt tcatcgtgta cagatacggt
      361 acgagtttat gacgcagacg aaggaaatca tccattgtgc tgcgcacttc catactgaag
      421 ttccaaggct cttttccact gaaggcactg ttagagctgt gcagacggtt gatcggagaa
      481 aagactccgt actgatacca tcttgcttca agctcatcgt cacggtagcc aagcatatgg
      541 ccgccgatat catgactcca ccatccgtag ccgatgttgg aggctgtgct ggtaaagtat
      601 ggctggaaat ccagggattc ccaggtgatg tgggtgtctc cggaaaatcc gaccggatag
      661 cggtgggagc ccggtcctgc atagcgggag aaggtcatcg gacgttttcc gtctcttcca
      721 ctgtcaagaa aatgatagtg attcagcatc cacagcggat caagaccctc gatcttggta
      781 ctctgtccct gctgccagtc aatccaccag aaatcgacgc cctctttttc catcggatga
      841 tggacttttt cgaaatatac atcgacaaat ttcggatccg caatgtcaaa attgaccggc
      901 tcttcttttt ttgcatccac tcccatagcc tctgctactt ccggatagca gtcctcatac
      961 gcacgaattc cgtctgccgg atggacattc agtgttgtct tcatgccgcg tgcatggagt
     1021 tttccaagaa aacgctccgg atccgggaag agttttttat tccagctata gccggtccag
     1081 ccgctgccgt atttcggatc cacatcgtct acacggtgcc agtccatatc gataacagca
     1141 accgagaatg gaacgttctc tttgtcgaag tcttccatca gtttcatata agatgcttca
     1201 ctgtaacgcc agtaacggct ccaccagttt ccgagtgcaa atctcggaag catcggagtt
     1261 ttgccgcaaa ggtgataaaa atcctttaat gccaggcgga aattccagcc atagccgaag
     1321 aagtacagat cctgcactcc tttctttctc ggggtcaccc aaccgtcctc tgtcatcaga
     1381 agcgacttcg aatcatccag aacagcggaa ccaaaccagg acatgatacc aggttccagt
     1441 tcgcatgctc cgttgacctg atcaagcgta cgggccgttc ctccaagatc ctgtgtcgga
     1501 tctccgtaat gccatgccgg tacagaactg atgccgactg ccgttgtttt gcaggaaagt
     1561 ccgtttgcag agaatgcctt tttgttgtaa ttcagctgca gatactttgt gcgcagctcc
     1621 agctcatcgc ctgtgtcttt gacggaaaat tccactgctg gaaaatcacg gtttacaacc
     1681 gtctgggatg ccgcatcgat aaattcaccg tcctcactgt attccagccg aagaagatgc
     1741 tccgtcagta ccgtgatacg atatttatcg ccctttacta cattcttcgg gtctgcaagc
     1801 ggatgtgtct ccaatttcag atatggtttc atgttttgtt ttcccctttc ttactcttac
     1861 cggtttttgc cgtattttga gaacgggaac ctgtggcaaa agtccgttct gccacaggtt
     1921 cctctgcatt ttttatttgg tttccagaac aactgcctca tacgcacgca gttttcttgc
     1981 ccccgctgct gcgccgctct gtggatagtt gctgatcagt acctctgctc ccgcaaaggt
     2041 ttccggaact gtgtactcct gctcctcttt tgtaaaattg cacatcacga ggagtttctg
     2101 gtcgcctaag gttctggtat atacatacag agcctcatcc tccggatcga gcagctcgta
     2161 cgttccgtaa accatgatat ccttctcttt ccggagatga atcagtttct tgtaataatt
     2221 gtatacagaa tccggatccg ccatctcatt cttggcattg acggtctcgt aatccggtcc
     2281 gaccttgatc cacggtgttc ctgtggtaaa tcctgccgaa gcggtatcat cccactgcat
     2341 cggagttctt gcattgtctc ttccgcgcag gttcaggtag cgcatcatgt cgtccggcgc
     2401 tacttttccg gctccggtca gctcgtggaa cgcgttgatg ctctcaatat cgcggaactc
     2461 ggacagatcg tggaacggaa catttttcat gccaagctca tcgccctggt atacatacgg
     2521 ggtaccctgc atcatatgaa ggcaggttgc cagcattttg gcacttacca cacggtattc
     2581 cggtgaatca tcgccgaaac gggacaggca gcgcggctgg tcgtggtttc caaggaagag
     2641 gctgttccat gccttgccct caagctccgt ctgccatgct gtcatgacgc gtttgaaatc
     2701 tttgagcggt acgcgtttgt ccgtccattt gccgtatgtt ccgcttgcca gatctaccag
     2761 ctcgaactgg aataccatgc tgagctcttt gccgtctgcg gatgcatatt tttctgcctc
     2821 ctcgatcgtg acaccggcac actcaccaac cgtcatcaga tcgtatttgg agaggacttc
     2881 cctgttcatc tcctgcagat actcatgaac gtgcggtccg tttacgcagc cggaatcacc
     2941 gtagagtccg ccctccggaa tttttccgtc cggcagccct tccggtttgg agatcatgct
     3001 gataacgtcc atccggaaac cgtcaatgcc tttttcacac caccagttca tcatatcgaa
     3061 aacttctttt cgaaccgccg ggttctccca gttcagatca ggctgttttg ttgagaacag
     3121 atgcaggtaa tacatctctg tctgcggatc gtatttccat gcggaaccgg agaagcagct
     3181 tccccagttg tttggctctt ttccctcttt tgcatcacgc cagatataat aatcgcggta
     3241 tggattgtct ttcgatttgc ggctctccac gaaccatttg tgttcatctg aagtatgatt
     3301 gacaacaagg tccatgacca gcctgatgcc tcttttatgc atctccgaaa gcatgcggtc
     3361 aaaatcctcc attgttccga actcctgcat gattgcccgg taatcgctga tatcatatcc
     3421 attgtcatcg tttggtgact ggtacaccgg agacagccag ataacatcca cgccaagatc
     3481 cttcagataa tccagacggg atgtgatacc atttaaatct ccgataccgt ctccgttgct
     3541 gtccatgaag ctgcgcggat aaatctgata cactacggct tctttccacc attttttgtc
     3601 cattttttta cctcctttat ttcatcccca gaacgtgtct ggttctttga ttctgtcgtt
     3661 tgcaagcaca ttttaccata cctcacggtg atttttcaat agttttgcgc aaaaccagca
     3721 gtttctacaa gagtttttct gtaaatttgg taactattta cttcgtcaga tctcttaccg
     3781 actccctctc caccagcgtt gtcggcaatg gcggaatatc tttttgtacc ggctcatcct
     3841 ggatcaagtg catcaggact tctacggcac ggcgtgcttt tccatccacc atctgatgca
     3901 cggtagtcag cttcggccgc gcaagtgtcg cataggtaac atcatcaaag cccgcaattg
     3961 aaatttcctc cggaaccttt attcccctgt tctgcagaaa gttggatgct tcaagtgcat
     4021 agtaatccga tgaaaaaaag agcgctgtct gttttttgaa gaacggaagc atctcctcat
     4081 atgccttctc cctctgctct ggattccacg ggatcacaat atggcgactt tcaaagtcct
     4141 caattcccgc ctcctccatt gcctgacgca caccgagcca ccgatagtgg tcaaccccga
     4201 gatcgcagtc ggataaaaag agaaattttt tatgtccctt tccaatcagg tactgactca
     4261 tctggtacgc accgccgcgg tcatccatcg taatggagcg gactccaagt tccggcggga
     4321 gatactgatc aatggttacc agcggttttc cgagacgttc cgagagctct gcaatctcct
     4381 tttccttcag agccatcgca atcatgccgt caaaattcca gccgagcgcc tgctgcataa
     4441 tatcgtcctg ctcatggcgc gcaaccagca tcatatagta gccgtgttcc cggatctgat
     4501 actccagatt tccgagcatt gccgacacaa agggatcctc aaaggaagat gcatttcctt
     4561 tcttcatgaa accaagcgcc acaccaataa gaccggagct gttgttcgcc agcatgcgcg
     4621 cgctgatact cggcacgtac tccatctcct ttaaaatccg gttgattttc tcgaccgtcg
     4681 cctttgagac gcgccctgtc tttccgtgta ttacattcga aaccgtcgtc gggctgaccc
     4741 ctgcgcggtc tgcaatatct ttgattctaa tcatcggttc tctgcctttc ctttttcttc
     4801 acaattttct gaattctgtt atgtttattt taccaagacg gcaaaatgtt tacaactact
     4861 ttgcatcggc aaacactaca aaattattac attttcttaa acgatatgta agaaaatctc
     4921 catccctcct tcatttactt tccgccggtt ccgtggtaag ataggatgac agaaaaagtt
     4981 cacctttttt ctgtaagaaa ggacctacac atcatatgcg gcttgcattt acaaattttt
     5041 cgttaaaatc tcccaccggc aagaaatccg gccggttttt tcttgccctt tttctgggca
     5101 tttttctctt tctgactcac cccatgctct gccaggctgg ttttgcctcc agtacgccgt
     5161 ttaccggtgc ctcctgggaa ctggtcgggg aatatccgat tctcggcagc aacggcgcgg
     5221 tgcagtccgt ctgtgcgacg gaagattata tcatctgcat cgaaaatttt aatgatctga
     5281 cgacagagcc ggacgtagtc tctgcctact ataaaaatga tacagacgca gacggaaatc
     5341 ccgttacgca gtattccctc gcgcatcagg taagggacgc agactttgct cacgcaaacg
     5401 gtatggcata caatcccgtc acacatgaaa tcctcgtgag cggctacagc agcccggatg
     5461 cttccaatta cggatgtatc ttccgcctcg acccggatac tctcgagcag aaagagcgga
     5521 tccagctctc gacaagctac aatattctcg gtattgatta tcttccctcc accggcggct
     5581 atctgatcca gaccgatgcg gacggcggct acggctttaa actcctcgat gtctcacttc
     5641 a
//
LOCUS       DAOMUF010000138         5631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3938_length_5631_cov_4.438307, whole genome shotgun sequence.
ACCESSION   DAOMUF010000138 DAOMUF010000000
VERSION     DAOMUF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3938_length_5631_cov_4.438307"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1484)
                     /gene="mgtE"
                     /locus_tag="PLN77_08055"
     CDS             complement(90..1484)
                     /gene="mgtE"
                     /locus_tag="PLN77_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRM44260.1"
                     /translation="MEKRVQDYTQEILDTIRSNSSPAVMGSRLEDYHENDLADVLPLL
                     TVPERCKLYRILDTDMLSDIFEYAESDNVAEYLEEMDVKKAASILSKMETDALAEVLQ
                     KLDKAKKKLLIELLDPEVRHDIEMIASFDEDEIGSRMTTNCIIIREDLNVKQAMSSLI
                     DQAAKNDNISTIFVVNAQQKFYGAIDLKNLIIARRDETLEDLTVTSYPYVYAEEPINE
                     CIEELKDYSEDSIPVLDNDNRLLGVITSSNIIDLVDDEMGEDYAMFAGLTAEEDLKEP
                     LKDSMKKRLPWLIILLGLGMLVSSVVGIFEQVVTTLPIIMTFQSLILDMAGNVGTQSL
                     AVTIRVLMDESLTGKQKLQLVFKEMRIGFCNGLLLGVLSFAVIGLFIFLFKGKTMMFA
                     FSVSACIGIALLLAMLISSAVGTCIPLFFKKIHIDPAVASGPLITTVNDLVAVITFYG
                     LSWLFLIEMLHLAG"
     gene            complement(1905..2306)
                     /locus_tag="PLN77_08060"
     CDS             complement(1905..2306)
                     /locus_tag="PLN77_08060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM44261.1"
                     /translation="MLAVYADLNNLKIINDRYGHEEGDFSIRLVGQFLQDSIGKRGVC
                     GRIGGDEFACLVPCSDETMSRDLYRTLHSAFDIFNRTSEKAYNITVSTGFYLLPKDMD
                     STLPEMLAHADAMLYEEKQKRDKNVAKILPK"
     gene            2555..3250
                     /locus_tag="PLN77_08065"
     CDS             2555..3250
                     /locus_tag="PLN77_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM44262.1"
                     /translation="MYNILVCDDDREIVDAIEIYLTQEGYHVLKAYDGMQAIQMLERE
                     EIQLLIIDVMMPKLDGIRATLKIREKSSIPIIILSAKSEDVDKILGLNIGADDYVTKP
                     FNPLELVARVKSQLRRYTKLGNLPADDGENVYQVGGLLVNDDLKEVSVEGEPVKLTPI
                     EYNILLLLVKNPGKVFSIEQIYESIWNEEAIGADNTVAVHIRHIREKIEINPKEPRYL
                     KVVWGIGYKIEKQ"
     gene            3267..>5631
                     /locus_tag="PLN77_08070"
     CDS             3267..>5631
                     /locus_tag="PLN77_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM44263.1"
                     /translation="MKERKLYSLTGKLIVFLLLIVFAASALFGGIQTLMLSSVGLTPN
                     AAAKKYAFEETTACGTLVQRQLHNVMSYYNDMSKFGTNGRYDGTKEIDYRAFATDGEG
                     SGGISTTYTIDQLVKMYDDGSSADLNEVAAYTADNYATNDAGIDKEAYLAYEDGTDVS
                     QETEETDVAALTEEVSAADAETFDSFSPDGEVYFPSDNAVKSLKEKKIDPADVSSQFL
                     YLYDQGMSLETQKTKAGTYLYEYAAANLKKVSIRDLYMTLSGVTEEVAAFKNQESGIS
                     VDADDANLWYCLEDKEGNIYTNVDSWKNGYTTAIAQKSPYPFAFAVDRTGGADKVRSY
                     ATRKNSPAVEAARSYLLNNQIADNYQKIFVALDPDLAASDYFSQYSEIYSQWLPKMQL
                     LLLMIAVGGIGTLVCLVLFTLQAGRNGKNKEIRLYFFDQWYTEIAAAAAILIVMGAVG
                     IVAVCIELSYGGVHYIQNFDGNYIYSGSYGNEADTWRSLVAASGILAGFLITLIPYCS
                     LVRRIKARNVWKQSFLYCVGHRIVSAGRTFYEGRQESERTILMFCVFCGVQIILCCGF
                     GMFGVFLTLVMDAAVLLLLLREASGRKQIEHGLKEIAAGDLDYKIDLTELQGEDNLQL
                     AGIVNSLGEGMKAAVQEQMKSERLKADLITNVSHDIKTPLTSIINYVDLLKRENIENE
                     KAKDYIRVLDEKSQRLKQLTEDLVEASKISSGNIKLEFMNLNLNELVQQVNGEFAERF
                     ESRNLDLICILQPEPLLICADSRRIWRVLENLYVNVCKYAMPGTRVYV"
BASE COUNT         1557 a         1264 c         1445 g         1365 t
ORIGIN      
        1 aggcaaatat cagctttttc cgaaaccaga aaaagttgat atctgcctct taaaagggca
       61 tcttttgttt tacggtacag attacgcggt cagcctgcca gatgcagcat ctcgatcagg
      121 aacagccagc taaggccata gaacgtaatg acagcgacaa ggtcgttgac cgttgtgatc
      181 agcgggccgg aagcaaccgc cggatcgatg tggatctttt taaagaacag cggaatgcag
      241 gtgccgacgg cactggaaat cagcattgcc agaagcagtg cgatgccgat gcaggcggaa
      301 acggaaaatg cgaacatcat ggtctttccc ttgaataaga agatgaaaag tccgatgaca
      361 gcaaaggaca gaacaccaag cagaagacca ttgcagaaac cgatgcgcat ttccttgaaa
      421 acaagctgta atttctgttt tccggtcagg gactcatcca tcaggacacg gatggtaaca
      481 gccagggact gggtaccaac gtttccggcc atgtcaagga tcagggactg gaaggtcatg
      541 atgatcggaa gggttgtgac aacctgttcg aaaataccga ctacagaaga aaccagcatg
      601 ccaagaccga gcaggatgat cagccatgga agacgttttt tcatactgtc ttttaacggc
      661 tcttttaaat cctcctctgc ggtcagacct gcgaacatcg cgtagtcctc acccatctca
      721 tcatcaacca ggtcgatgat gtttgatgat gtgatgacac cgagtaggcg gttgtcattg
      781 tcgaggaccg gaatagaatc ctcggaataa tccttcagtt cttcgataca ctcgttgatc
      841 ggctcctctg catatacgta cgggtaagaa gttacggtca gatcctcgag cgtctcgtcg
      901 cgtcttgcga tgatcaggtt tttcagatcg atcgctccgt aaaatttctg ctgcgcattg
      961 acaacaaaga tggtagagat gttgtcgttc tttgcggcct gatcgatcag gctgctcatc
     1021 gcctgtttga cattcagatc ctcgcggatg atgatgcagt ttgtcgtcat gcggctaccg
     1081 atttcatctt catcgaagga ggcgatcatc tcaatatcgt ggcgtacttc cggatcgagc
     1141 agttcgatca gaagtttttt ctttgctttg tcgagtttct gcagaacctc ggccagtgca
     1201 tcggtctcca ttttggagag gatggaagcg gcttttttga catccatctc ttcgaggtac
     1261 tcggctacgt tgtcggattc agcatactca aaaatatcgg acagcatgtc cgtgtccaga
     1321 atgcggtaca gtttgcatcg ctccggaacc gttaaaagcg gcagaacatc tgccaggtca
     1381 ttttcgtggt aatcttccag gcggctgccc atgacagccg gagaagagtt actgcggatt
     1441 gtatccagga tctcctgggt ataatcctgt actcgttttt ccattttttc gttatcctcc
     1501 attctttttt ttgacagcag aaaaaaacat gcgtgctgtc ggtgcgaaaa agcatgttgg
     1561 aaccctcaca gggcagtaaa agcacacgaa aaaagaacca gagaattacg tttcaaaact
     1621 ggtttggatc atgtgtctta cttcgggttt tcataggata agaagggaaa atatttaaga
     1681 ttaaagcagg acagaaggat ggccgtcatc ctgcatattt tccggttccc gtcctatgct
     1741 ggtacttcgc ccatgagaat cacaactatc cattctgttc acctgccttt ttttagaaat
     1801 tttttgatca aacagccgtt tttgccggtc tgtttcgctt gaaatacggg attcaaaaat
     1861 catccctcaa gtattctaac tgctttccag aaaaaaatca attcctattt tggtaaaatt
     1921 ttcgcaacat tcttatctcg tttctgtttc tcctcgtaaa gcattgcatc cgcatgcgcg
     1981 agcatctccg gaagtgtgga atccatgtct ttcggaagca gataaaagcc ggtggaaacg
     2041 gtaatattat atgctttttc gctcgttcta ttaaaaatgt cgaatgcgga gtgcagcgtc
     2101 cggtacagat ccctggacat tgtttcgtcg gaacacggaa caagacatgc gaattcatcg
     2161 ccgccgatgc gtccgcagac accgcgtttg ccgatggaat cctgcagaaa ctgtccgaca
     2221 aggcggatgg agaagtcccc ttcttcatgc ccgtaccggt cgttgatgat ctttaaatta
     2281 ttgagatccg catagacggc aagcaggatg tgctaagaaa ataattgcaa ttgtctaaaa
     2341 aatgtttgag ctctgaattc acagacaaaa ctttatagaa acttaagatt ttcctttgcg
     2401 gacctaaaga ttcctttggt acagtaagga tgaaaacaaa agacatctgg caacttgagg
     2461 gaatttgcgg tacaatcatc tttgaatcat gtgggaggtc cggcgcggaa aaaaagtgcc
     2521 ggaaaagaaa aaatgaaagg tggagaaaga agaaatgtac aacattttag tatgtgatga
     2581 tgatagagag attgtagacg ccattgagat ttatctgacc caggagggct atcatgtgct
     2641 gaaagcatat gacggcatgc aggccatcca gatgctcgag agggaggaga ttcagctgct
     2701 gattatcgac gtcatgatgc cgaaactgga cggcatccgt gcgacgctga aaatcagaga
     2761 aaagagcagt attccgatca ttattctgtc tgcgaaatca gaggacgtag acaaaattct
     2821 gggactgaac atcggcgcgg atgattacgt gacgaagccg ttcaatccgc tcgagctggt
     2881 ggcgagggtg aaatctcagc tgcggcgcta cacgaaactt ggaaatcttc cggcggacga
     2941 cggagaaaat gtgtatcagg tcgggggtct tctcgtcaac gacgatttaa aagaagtcag
     3001 cgtcgagggc gaaccagtca aactgacccc gatcgaatac aacattctcc tgctgctggt
     3061 aaaaaatcca ggcaaggtat tttcgattga acagatctac gagagcatct ggaacgagga
     3121 ggcgatcggt gcggacaaca ccgttgcggt ccacatccgt cacatccgtg agaaaatcga
     3181 aatcaacccg aaggagccgc gctatctgaa agttgtatgg ggaattgggt ataagattga
     3241 gaaacaataa taaggagtga ataattgtga aagaacgaaa actctacagc ctgacgggga
     3301 agctgatcgt ttttcttctg ctgatcgttt ttgccgccag tgcgctgttc gggggcattc
     3361 agacactgat gctttccagc gtcggcctga cgccgaatgc ggcagcaaaa aagtatgcgt
     3421 ttgaagaaac caccgcatgc ggcacactgg tgcagcggca gcttcataac gtcatgagct
     3481 actataatga tatgagtaaa ttcggcacaa acggtcgata cgacggaaca aaagagattg
     3541 attaccgtgc ctttgcaacc gatggagagg gaagcggcgg gataagtaca acgtacacca
     3601 ttgaccagct tgtcaagatg tacgatgacg gcagcagcgc agacctcaat gaggtggcag
     3661 cgtatactgc ggacaattat gcgacgaacg atgccggaat tgacaaagaa gcctatcttg
     3721 catacgaaga tggaactgac gtgagccagg aaacagaaga gacggatgta gccgcactca
     3781 ccgaagaagt gagcgcggca gatgcagaga cgtttgacag cttttccccg gacggcgagg
     3841 tctactttcc ttccgataat gcagtgaaaa gcctgaaaga gaaaaaaatt gatcctgctg
     3901 atgtttcctc ccagtttttg tatctatacg atcagggaat gagcctggaa acgcagaaaa
     3961 cgaaagcggg aacgtacctc tatgagtacg cggcggcaaa tctgaaaaag gtatccatcc
     4021 gcgatctgta tatgacactt tccggggtca cggaagaagt tgctgctttt aaaaaccagg
     4081 agagcggcat cagcgtcgat gcggacgatg ccaatctctg gtactgtctg gaggataaag
     4141 aaggaaatat ctatacgaat gtggattcct ggaaaaatgg ttacacaaca gccattgcgc
     4201 agaaatcgcc ctatccgttc gcctttgctg ttgaccggac cggcggtgcc gacaaagtcc
     4261 gttcttatgc aacaagaaaa aacagcccgg ccgtggaagc agcgagaagc tatctactga
     4321 acaatcagat tgcagataat tatcagaaga tctttgttgc actggatccg gatctggcag
     4381 caagcgacta tttctcccag tacagtgaga tctacagcca gtggcttccg aaaatgcagc
     4441 tccttcttct gatgattgca gttggcggaa tcggaacgct ggtctgcctg gtcctgttca
     4501 cactgcaggc aggaagaaat ggaaaaaaca aagaaatccg cctttacttt ttcgatcagt
     4561 ggtacacgga aatcgcagct gcagccgcca tcctgattgt gatgggagca gttggaatcg
     4621 ttgctgtctg catcgaactt tcctacggcg gcgttcatta tatccagaat tttgacggaa
     4681 actacatcta cagcggatcc tatggaaatg aagcagatac atggcgaagc ctggtggccg
     4741 catcgggcat cctcgccgga tttctgatca cactgattcc gtactgctca ctggtgcgcc
     4801 ggatcaaagc acgcaacgtc tggaaacagt cgtttttgta ctgcgtcggc caccggatcg
     4861 tgtctgccgg aagaaccttc tatgaaggca gacaggagtc ggagcgaacg atccttatgt
     4921 tctgcgtttt ctgcggcgtg cagatcattc tctgctgcgg cttcggaatg ttcggcgtat
     4981 tccttaccct ggtgatggat gccgcagtgc tgcttcttct gctgcgcgag gcatccggaa
     5041 gaaaacagat tgaacatggc ttaaaagaga ttgctgcggg cgatctggat tacaagattg
     5101 acctgacgga gctgcagggg gaagacaacc ttcagcttgc cggaattgtc aattcgctcg
     5161 gagagggaat gaaagcggca gtgcaggagc agatgaagag cgagcgctta aaagcggatc
     5221 tgatcaccaa cgtatcccac gatatcaaga caccgcttac ttctattatt aattatgtcg
     5281 atctgctgaa acgggaaaat attgaaaacg agaaagcgaa agattatatc cgcgtactcg
     5341 acgaaaaatc acagcgcctg aaacagctga cggaggacct tgtcgaggca tcgaagatca
     5401 gttccggaaa tattaaactg gaatttatga acctgaacct gaacgaactt gtacagcagg
     5461 tgaacggaga gtttgccgag cggtttgaaa gccgtaatct tgatctgatc tgtattctcc
     5521 agccggagcc gcttctgatc tgtgcggaca gccgccgcat ctggcgtgtg ctggaaaacc
     5581 tgtacgtaaa tgtctgcaaa tatgccatgc cgggaacacg ggtttatgtg g
//
LOCUS       DAOMUF010000139         5594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3972_length_5594_cov_3.202383, whole genome shotgun sequence.
ACCESSION   DAOMUF010000139 DAOMUF010000000
VERSION     DAOMUF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3972_length_5594_cov_3.202383"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PLN77_08075"
     CDS             complement(<1..139)
                     /locus_tag="PLN77_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44264.1"
                     /translation="MAGYVSLQDVTKTYQMGEITIKAADGISFDVEKGEFVIVVGPSG
                     AG"
     gene            complement(245..400)
                     /locus_tag="PLN77_08080"
     CDS             complement(245..400)
                     /locus_tag="PLN77_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44265.1"
                     /translation="MMRSGNIFEREGSRGHGCDGDGTEITGGGDYSGWDKMYDQGSIE
                     IKGGCHL"
     gene            648..1364
                     /locus_tag="PLN77_08085"
     CDS             648..1364
                     /locus_tag="PLN77_08085"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM44266.1"
                     /translation="MKIIFIRHGEPNYEIDSLTEKGWREAELLSKRTAKWNVTDFYCS
                     PLGRAKDTASFTLKNADREAKILPWLREFDAPVIDPETGKRRIPWDFLPDVLDANRDL
                     YDNHLWTKNPIMQTGNMDENYRFVTDGLDTLLAHYGYVRDGYRYHASDETNREAVIVC
                     FCHLGVTCVALSHLLNMTPTQLWQGMFLAPTSVTIVGSEERKPGEVYFRCQTVGDVHH
                     LLTAGEPVSYYGSFNDPFQF"
     gene            1391..2623
                     /locus_tag="PLN77_08090"
     CDS             1391..2623
                     /locus_tag="PLN77_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM44267.1"
                     /translation="MNEAKLREYAKLIVKIGANVQKGQRVRLQAGVDQVLLATMVTEE
                     CYKAGASYVELIWECGPINKLSYQYATAETLGTVQNWEEERLKQMTVDLPVRIFIDSS
                     DPDELAGISADLLSTVRQMRGKVTKKYRDQLDGKHQWLIVAAPSPAWARKVFPGEPED
                     IAVEKLWNAIFDCLYLKEGEDVEKIWQAHCDRMTQKANWLNEQAFRKLHYTSKNGTDF
                     TVELIPDAKWSGAGDINHMNNTFYVPNMPTEEVFTSPMRGKCEGRLVSTKPLSWSGQV
                     INNFTVDFKDGKVVDCHAEQGEEVLKKMFAMDEGAAMLGEVALVPKESPINQSGLMFF
                     NTLFDENACCHVAAGAGFSEVLDGFMDMSDEEILAKGINDSLIHVDFMVGSDDLHIVG
                     IHEDGSETDVFVNGTWAE"
     gene            complement(2633..3241)
                     /locus_tag="PLN77_08095"
     CDS             complement(2633..3241)
                     /locus_tag="PLN77_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HRM44268.1"
                     /translation="MNILWSSMLLLGIAYGALQGRMPEVTDGVIQASREAVVLAVSLV
                     GVTGFWAGLMEIAKKAGVIDGLVKRMGPVMRFLFPEVPEDHPAMRAAGMNMICNVFGL
                     GAAATPPGLAAMERFEELEEERRKMRNPAAVPEGTANREMCTFLILNISSLQLIPVNI
                     IAYRSQYGSVHPTAIVGPALAATAASTIAAVVFCKVMERMGR"
     gene            complement(3545..4912)
                     /locus_tag="PLN77_08100"
     CDS             complement(3545..4912)
                     /locus_tag="PLN77_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44269.1"
                     /translation="MSQTQTRRSSQVDLLHGPILKSLLLFALPIMASRVFQQIYNTMD
                     TLIVGYTLGDSSIAAMGACSSIYEMMTGFTFGVGNGLAIVTARCFGRQETDRMKKSVA
                     SCIVIGVAVTLVVTILATIFMHPLLRLLKTPAEILEESYSYISILSIFLFVMFAYNLC
                     SGLLRAIGNSFMPLVFLIFSSLVNIVLDLLFIRTFGMGVRGAAIATVIAQGISVVLCL
                     IYIVTKAKILLPEKRHFALDRGLYADLLAQGFSMGFMSSIIYVGSIVLQPGINSLGYE
                     TIAGHTAARKLFSFSMMPLSSMAQAVNTYVAQNRGADNWERVRKGIRYAYIFCFSVTA
                     CLIVIVTPLAPTMVHLISGSSDPTVLGNGARYLRVAIPFFFALGLVNCSRLALQALGE
                     KILPLLSSFIELFGKIIFAMVFIPRYHYSAVIWCEPIIWCFMAAELVISLYRNPYMRK
                     GKTGN"
     gene            complement(5026..>5594)
                     /locus_tag="PLN77_08105"
     CDS             complement(5026..>5594)
                     /locus_tag="PLN77_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM44270.1"
                     /translation="LAEQLHVVRQTISKWEKGISVPDAVMLDRMAELFEVPVSVLLGG
                     GLEVEEEQPSELNEIAQQLAVLNDQLVQQAVRRRKVIRYAFVGVFAAIFVLIGAAIGL
                     RVYTESQLRDEASLRTVRYECTLDGESYVYEASYNSQYQILYEGGDAWISNHVRPEQY
                     EDVNVLSAQMQDYFEEKGGTCTIIDENP"
BASE COUNT         1460 a         1533 c         1299 g         1302 t
ORIGIN      
        1 tacccgcacc gctcggtcca acaacaatca caaactcacc cttttccaca tcaaagctga
       61 tcccatccgc agccttgatg gtaatctcac ccatttgata ggtcttggtt acatcctgaa
      121 gactcacata tcccgccatg ccgccgtgtt cctcctatgc aaaaaatcga tatttcctat
      181 ttcttttatt gtatcaatct acagccgcgg cggctagtcc taagtggacg aatttactgt
      241 ttttttataa atggcatccc ccctttattt caatactgcc ctgatcatac atcttatccc
      301 aaccagaata atcaccacct cctgtaatct ctgtgccatc tccatcgcag ccatgccctc
      361 tgctgccttc ccgttcaaaa atatttccag aacgcatcat cctctgtcac tctcctctca
      421 aatcttaacc actcctccca ggccgcctac cagacgtgcg aacgcctctt ccaatttgcc
      481 acatcttttt ccacagaaac tcacacaagt ttgacaattt atgatacact aagctaagtg
      541 acatcaattc tgaatgttct gtgctctgcc gcagattttt cggaactgaa attgactttt
      601 cgatgcataa accaaacgat ttgtggcata cgaagaaagg aattcttatg aaaattatct
      661 ttatccgcca cggcgaaccg aattatgaga ttgattcgct gactgaaaaa ggctggcgtg
      721 aggctgagct gctttcgaag cgaacggcga aatggaacgt caccgatttt tactgctcgc
      781 cgctcggacg tgcgaaggat acggcttctt tcacactgaa aaacgcggat cgcgaagcca
      841 aaattcttcc atggctgcgc gaatttgatg cgcctgtcat tgacccggaa accgggaagc
      901 gccggattcc gtgggatttt ctgccggatg tactcgacgc aaaccgggat ctctatgaca
      961 atcatctctg gacgaaaaat ccgatcatgc aaaccggaaa tatggacgaa aattaccgtt
     1021 ttgtgaccga cggactcgat acgctgcttg cacactacgg ctatgtacgg gacggctatc
     1081 gctatcatgc ctctgatgag acgaaccgcg aggctgtcat cgtctgcttc tgtcatctcg
     1141 gcgttacctg cgtggcactg tcccacttgc tgaatatgac accaacccag ctctggcagg
     1201 gtatgtttct ggcgccgact tctgtcacaa tcgtcggaag tgaggaacgc aaaccgggcg
     1261 aagtgtactt ccgctgccag actgttggcg acgtacacca tctgctgact gcaggcgagc
     1321 cggtttcgta ctatggatcg tttaacgatc cattccagtt ttaaacttta caaaaaggga
     1381 gttttttatc atgaatgaag caaaattaag agaatacgca aagctgatcg taaaaatcgg
     1441 cgcaaatgta cagaaaggac agcgtgtccg tctgcaggcc ggtgtggatc aggttctgct
     1501 tgctaccatg gtcacggaag agtgttacaa agccggtgca agctatgttg agctgatctg
     1561 ggagtgtggt ccgattaaca agctgagtta tcagtacgcc acagccgaaa cactcggtac
     1621 ggttcagaat tgggaggagg agcgcctcaa acagatgacc gtagatcttc cggtccgcat
     1681 tttcattgac tcctccgatc cggatgagct tgccggcatc tctgccgatc tgctctctac
     1741 cgtccgtcag atgcgcggga aagtaacgaa aaaataccgc gatcagctcg acggaaaaca
     1801 tcagtggctg atcgttgccg ctccgtcacc ggcatgggcc agaaaagtat tcccgggcga
     1861 gccggaggat attgcggttg agaagctgtg gaatgctatt tttgattgcc tgtatttaaa
     1921 agaaggcgaa gatgtcgaga aaatctggca ggcacactgt gaccgcatga cccaaaaagc
     1981 caactggctg aatgaacagg cgttccgcaa actgcattat accagcaaaa acggcaccga
     2041 tttcacagtt gaactgattc cggacgcaaa gtggagcggc gctggcgata tcaaccatat
     2101 gaacaatacc ttctatgtcc cgaatatgcc gaccgaagaa gttttcacca gcccgatgcg
     2161 cggcaaatgt gaaggccgcc tcgtttccac caaaccgtta agctggtccg gccaggttat
     2221 caacaacttt accgttgatt ttaaggacgg aaaggttgtc gactgccacg cagaacaggg
     2281 cgaagaggtt ctgaagaaaa tgttcgcgat ggatgagggc gccgcgatgc tcggcgaggt
     2341 tgcactcgtt ccgaaggagt ccccgatcaa ccagtccggt ctgatgttct ttaacactct
     2401 gttcgacgag aacgcatgct gccacgttgc cgccggcgcc ggcttttccg aggtgctgga
     2461 cggatttatg gatatgagtg atgaagaaat cctcgcaaaa ggtatcaacg attccctgat
     2521 ccacgttgac tttatggttg gatcggatga cctgcatatc gtgggtattc acgaagacgg
     2581 aagcgagacc gatgtctttg taaacggcac ctgggctgag taatctgaat aatcatcgcc
     2641 ccattctttc catcactttg caaaacacca ccgccgcaat cgtactcgca gccgtcgcag
     2701 caagcgccgg tccgacaatt gctgttgggt ggacgctgcc gtactgactg cggtacgcaa
     2761 tgatgttgac ggggatgagc tgcagagaag aaatatttaa aatcaaaaac gtacacattt
     2821 cccggtttgc ggttccttct ggaactgcgg ccgggtttct cattttccgc cgctcttcct
     2881 ccagctcctc aaaccgctcc atcgccgcaa gccccggcgg cgtcgcagcc gcgccgagtc
     2941 caaacacatt acagatcata ttcatccccg ccgcccgcat cgcaggatgg tcttccggca
     3001 cttccggaaa cagaaaccgc atcaccggcc ccattcgttt caccagcccg tcaatcactc
     3061 ccgccttctt cgcaatctcc atcagccctg cccagaaccc cgtcacccca accagcgaaa
     3121 ccgccagcac caccgcctcc ctcgaagcct gaatcacccc atccgtcacc tccggcatcc
     3181 gtccctgcag cgcgccatat gcgattccga gaagcagcat ggaactccat agaatgttca
     3241 ttgcatcacc tgactaaaaa gatctttata aaaatatgct gactttatat tttccatgat
     3301 tcgaaactca agtatacacc ataatcccac aaatttttat tatgccgtcg ttttataatg
     3361 ctttctaaaa ttgatatttt agttttatac gtatataata ataaacatta taagggtggt
     3421 gttttaaaat gaagaaatga gacctgcatt cagtgcctgc tatgaataat ataatgtgat
     3481 atataattac cggtcaacac acagattttt ccgtgcattg gccggtaatt ttttcattta
     3541 aaaatcagtt tcctgtcttc ccttttctca tataaggatt tcgatacagt gaaatcacaa
     3601 gctccgcagc catgaagcac cagatgatcg gttcgcacca gattacggcg ctgtagtggt
     3661 agcgtggaat gaataccatg gcaaagataa tctttccaaa cagctcaata aagctcgaca
     3721 gcaacggcag aattttctct ccgagtgcct gcagtgccag acggctgcag ttgacaagac
     3781 caagggcgaa gaaaaacggg atggctacgc ggaggtagcg ggctccgttt ccgaggacgg
     3841 tcgggtcgga tgaaccagag atcaggtgta ccatggttgg tgcaagcggg gtgacgatta
     3901 caatcaggca cgcggttacg ctgaagcaga aaatgtaagc gtaacggatg cctttccgca
     3961 cgcgctccca gttgtctgcg ccgcggttct gtgcaacgta ggtgttgacg gcctgcgcca
     4021 tggaggaaag cggcatcatg gagaacgaaa acagctttct cgcagcggta tgtccggcga
     4081 tagtttcata tccgaggctg ttgatgcccg gctgcagaac gatcgaacca acataaatga
     4141 tgctgctcat aaatcccatc gaaaatccct gcgccagcag atctgcgtac agtccgcggt
     4201 ccagcgcgaa gtggcgtttt tccggaagca ggattttcgc tttcgtaaca atataaatca
     4261 ggcagagcac aacggaaatg ccctgtgcaa tgacggttgc aatcgcggca ccccggacac
     4321 ccatgccgaa ggtgcggata aacagcagat cgagcacgat gttcaccagc gatgaaaaga
     4381 tcaggaacac aagcggcatg aagctgttgc cgatcgcccg cagcaggccg gagcacaggt
     4441 tgtaggcgaa catgacgaac aggaaaatcg agagaatcga aatatagctg taggattcct
     4501 caagaatctc cgccggtgtt ttcagcaggc gcagcagcgg atgcatgaag atcgtcgcca
     4561 gaatggtaac taccagtgtc acggcaacgc cgatgacgat acaggatgcg accgattttt
     4621 tcatccggtc cgtctcctgc cgtccaaagc agcgcgccgt gacgatcgca agaccgtttc
     4681 cgacgccgaa cgtaaatccg gtcatcatct catagatgga tgagcaggca cccatcgccg
     4741 caatcgatga gtcgccaagt gtatagccga cgatcagcgt atccatcgtg ttgtaaatct
     4801 gctgaaacac gcgggacgcc atgatcggca gggcaaaaag cagcagagat ttcaggatcg
     4861 ggccgtgaag cagatcgacc tgagaactcc gccgggtttg tgtctgtgac ataaaaaaat
     4921 tcctccctct aagtataatg cattgttacc attatatcag aagagaggaa ttaccgggca
     4981 gataaaatac ccaaaaccat aaaaaaatac aataatacaa aacttttacg gattttcgtc
     5041 aatgatcgtg catgttccgc ctttttcttc gaagtaatcc tgcatctgcg ccgacagcac
     5101 attcacatcc tcgtactgct ccggccgcac atggttcgaa atccaggcat cgccgccctc
     5161 gtacaggatc tgatactggc tgttatagga agcctcatat acatatgatt ccccgtcgag
     5221 cgtgcattca taacgaaccg tgcgaagcga cgcttcatcc cgcagctgtg attccgtgta
     5281 cacccgaagt ccgatcgccg ctccaatcag aacaaagatc gccgcaaaca caccaacaaa
     5341 cgcataccgg atcactttcc gtctccgcac tgcctgctgc accagctgat catttaaaac
     5401 cgcaagctgc tgtgcaatct cattcagttc cgaaggctgc tcctcctcta cttccagccc
     5461 gccgcccagc agcacactca ccggcacctc aaacagctcc gccatccgat ccagcatcac
     5521 cgcatccgga accgaaatcc ctttctccca tttcgaaatc gtctgccgca ccacatgcag
     5581 ctgctccgcc agcg
//
LOCUS       DAOMUF010000140         5582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_3988_length_5582_cov_2.715397, whole genome shotgun sequence.
ACCESSION   DAOMUF010000140 DAOMUF010000000
VERSION     DAOMUF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_5582_cov_2.715397"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..582)
                     /gene="atpF"
                     /locus_tag="PLN77_08110"
     CDS             complement(79..582)
                     /gene="atpF"
                     /locus_tag="PLN77_08110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRM44271.1"
                     /translation="MQVQELVGIVPWTFIAQICNLFIQMYLIKRFLFKPINEMLEKRR
                     AAADAEITDAEAAKKEANAIKEEYEQNMQEAKAKANEILESARKTATLQSEKIVKEAS
                     EQAAALKNKAEKEIAQEKKKAVNEVKGEIGGIAMDIAGKVIEREINEKDHEKLIDEFI
                     SNVGEAS"
     gene            complement(620..898)
                     /gene="atpE"
                     /locus_tag="PLN77_08115"
     CDS             complement(620..898)
                     /gene="atpE"
                     /locus_tag="PLN77_08115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRM44272.1"
                     /translation="MDFQILAKGIALAGCAIGAGCSLIAGIGPGIGEGNAVAKALEAI
                     GRQPECKGDVTSTMLLGCAIAETTGIYGFVTGLLLIFVAPGMFMNFLK"
     gene            complement(949..1710)
                     /locus_tag="PLN77_08120"
     CDS             complement(949..1710)
                     /locus_tag="PLN77_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HRM44273.1"
                     /translation="MEVDISGAKVFFTIPIDFPLFGKIQISETLVVSWIVMALITGLC
                     IWLTRDLKIRNISKRQAVAEMIVETANKFVIGNMGEKFRYLIPFVSALFATSVVSNLI
                     SLIGLRSPTADLSTEAAWAVVVFIMITTQKIKTNGFGGYLKGFTTPIAVMTPFNVLSE
                     LATPISMACRHFGNILSGVVINALIYGSLALASGKLLGLLPGVLGRTLSQIPILDVGV
                     PAVLSVYFDWFSGVMQAFIFCMLTVMYIANAAEEG"
     gene            complement(1877..2299)
                     /locus_tag="PLN77_08125"
     CDS             complement(1877..2299)
                     /locus_tag="PLN77_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44274.1"
                     /translation="MSNFGDKVQPAVKKETKNVAIYTVVGVVVMWVGFLILHLIWPEK
                     IPFDYRVILGGIGGGAVAVLNFFLMGMAVQKAVSETDEAGAKRRMQASYSQRNLMQML
                     WAILAIVLPCFQFAAGILPLLIPGTAIKLMGIKNSIKR"
     gene            complement(2404..2688)
                     /locus_tag="PLN77_08130"
     CDS             complement(2404..2688)
                     /locus_tag="PLN77_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRM44275.1"
                     /translation="MKKWTGMITNLTMLTQFGLSLVTPLFLCLALCWWLCDRFSVGTW
                     IYLLGFFFGIGGGAVTAWKFGQSVLRHQKRIETEPDGKKKKKRVSFNRHI"
     gene            complement(2956..5553)
                     /locus_tag="PLN77_08135"
     CDS             complement(2956..5553)
                     /locus_tag="PLN77_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HRM44276.1"
                     /translation="MKIYHAQVNHLENPVGFRMERTVFSWKIKEAKGKQQSYARIRVS
                     SDEAMEHLLFDSGEDTKASSLCYPVKLKLEPRTRYYWTVEAGSDAGEKAVSEVQFFET
                     GKREEAWSAKWVSCSSEEKRHPYFEKKIIPAKEVAKARLYICGLGLYEVFVDEKRVGN
                     EYLTPYSNDYNEWVQYQTYDVTEEFSSEGTLRVLLGNGWYKARFGFSAFEDRGFYGND
                     WKLIAELHLMYTDGSEEVIGTDETWQIRRSKISFSNLYDGEHRDDTLPDLPAEQAVLC
                     DAPKGELTDRMSLPVTVHETFRPKELIHTPAGELVFDMGQEFTGIFKLHVNVPKGTKV
                     HVQTGEILQHGNFYNENLRSAKSEYIYISDRTEIDLVPHFTFYGYRYVKIEGIPDLKK
                     EDFTGLAYYSNITATGWMKTGSDLVNQLISNVRCGLKCNFVDVPTDCPQRDERMGWTG
                     DAQVFSPAAMYLEDTYAFYAKYLYDMAKEQSVLGGKVPHVVPSCGVEDAACVWGDAAC
                     IIPWNLYLFYGDKSILEDQFVSMKSWVDYITKVDGDNHGWRYIFHFGDWLALDNPVQG
                     AEQVMGATDEEFIANLYYAISAGIVAKAAGVLGYREEQEKYQKLSEEQFAVVQEEYYS
                     ATGRCCIKTQTALLLTLKYHLSKNEELTKRQLLKLFEQSNHKLKTGFVGTPLLNNVLT
                     DNGMNDLAYELLLNEEFPGWLYEVKLGATTVWERWNSLLTDGTISGISMNSMNHYAYG
                     SIQEWMFRHVAGINTMESHPGARTVQFAPTLNWDLRYAEAKYDSASGMYSIRWELSDK
                     EHVTITMDVPFDCTAEAVLPMVAKSEKEAVAEVLGSEENGRYLLEPGHYEVSYQLSGW
                     KEKTAVCVE"
BASE COUNT         1282 a         1564 c         1125 g         1611 t
ORIGIN      
        1 cggaaaggat ttcctcctcg agatgctcct ctgcggccag ctcatacagg cttccaccgt
       61 atactctggc agtttctgtc atgatgcttc acctacattc gatataaatt catctatcag
      121 cttctcatgg tctttttcat taatctcacg ttcaatgacc tttccggcaa tatccatggc
      181 aatgccgccg atctcgcctt tgacttcatt gaccgctttc tttttctcct gggcaatctc
      241 tttttctgcc ttgtttttca gggcagccgc ctgttcggaa gcctctttta cgattttctc
      301 gctctgcagc gttgctgtct ttctggcgct ttcgagaatc tcgttggcct ttgcctttgc
      361 ctcctgcata ttctgctcat attcctcttt gatggcgttg gcttcttttt ttgccgcctc
      421 tgcgtctgta atctccgcat cggctgccgc acgccgtttt tccagcatct cattgatcgg
      481 tttgaaaagg aaccgtttga tcagatacat ctggatgaag aggttgcaga tctgtgcgat
      541 aaaagtccag gggacaattc ctaccagttc ctgaacctgc atagtttctt tcctcctagc
      601 aatcatttac tccttgcaac tatttcagga agttcatgaa cattcccggt gctacgaaga
      661 tcagaagcag gccggttacg aaaccgtaaa tacctgtggt ctctgcgatc gcacagccaa
      721 gaagcatcgt acttgttaca tcacctttgc attccggctg acgtccgatt gcctcaagtg
      781 cctttgctac ggcgttacct tcaccgatac caggtccgat acctgcgatc agggagcatc
      841 ctgcgccgat tgcgcatcct gcgagagcga taccttttgc taaaatctga aaatccatct
      901 tgttttcctc ctgtattttc tttttgtaaa cctcattttt taatctcttt agccttcctc
      961 tgctgcattc gcaatgtaca tgacagtcag catacagaaa ataaaggcct gcatcacgcc
     1021 ggagaaccag tcaaaataga ccgacagaac agccggaaca ccgacgtcca gaatcgggat
     1081 ctgtgaaagg gttcttccga gcacgccggg aagcagtccc agcagctttc cgcttgccag
     1141 tgccagcgat ccgtagatca gggcattgat gacaacgccg gaaaggatgt ttccaaagtg
     1201 acggcacgcc atactgatcg gcgttgcgag ctccgacaaa acgttaaacg gtgtcataac
     1261 cgcaatcgga gtcgtgaatc ctttcagata tccgccaaaa ccgttggttt tgatcttctg
     1321 tgtggtgatc atgatgaaaa ccacaacagc ccaggccgcc tcggtagaaa gatctgctgt
     1381 cggacttcga agtccgatca ggctgatcag gttcgagaca acgctggtcg cgaaaagcgc
     1441 cgacacaaaa ggaatcagat agcggaattt ttctcccatg ttgccgatga caaatttgtt
     1501 tgcggtttcc acaatcattt ctgccaccgc ctggcgtttg gagatgttcc gtatcttcaa
     1561 atctcttgtg agccagatgc aaagtccggt aatgagtgcc attacaatcc agcttaccac
     1621 cagcgtttcg ctgatctgaa ttttcccgaa cagcgggaaa tcgatcggga tcgtaaaaaa
     1681 gacttttgca ccggagatat caacctccat tctcttttct cacctcctct gtatgagata
     1741 aaataaaaaa tttgaacctt ttgtcaaagc ggatcttcac aatccgcttc gctgcttgct
     1801 cataaccgaa tcgctgcttc gctgtttcgg tttaaaaacg ccgtcccgtc tttgcggctt
     1861 tcgcctgaga tcaggttcag cgctttatgc tgttttttat gcccatcagc ttgatggccg
     1921 ttcccggaat taaaagcggc aggattcctg cggcaaactg gaagcatgga agcacaatcg
     1981 ccagaattgc ccagagcatc tgcatcaggt ttctctgcga atagcttgcc tgcatccgcc
     2041 gttttgctcc cgcctcgtct gtctcggaca ctgctttttg tactgccatg cccatcagga
     2101 aaaagttcag caccgcaacg gcaccgcctc cgattccccc aaggatcacc cgatagtcaa
     2161 atgggatttt ttccggccag atcaggtgca ggatcagaaa cccgacccac atcacaacca
     2221 cgccgacaac agtatagatg gcaacatttt tcgtctcctt ttttacggcc ggttggacct
     2281 tgtctccgaa attgctcata tcgccttgtt tcctttctaa gggattttcc ccttaaaatc
     2341 agtctgaaat cttttctctg cctcttgatc ttcgtcaact gtgaaaactt tttcagacac
     2401 gttctaaata tgtcggttga aagacaccct ctttttcttc ttttttccgt ccggctccgt
     2461 ctcgatgcgt ttctgatgcc gcagaacgct ctgcccgaat ttccaggctg tcacggcacc
     2521 gcctccgatc ccaaagaaaa agccgagcag ataaatccat gttccaaccg aaaagcggtc
     2581 gcaaagccac cagcaaagtg ccagacaaag aaaaagaggc gtcacaaggg acagtccgaa
     2641 ttgtgtcaac attgtgagat tggtaatcat ccccgtccac ttcttcatat cctgtccctt
     2701 tctgccgcct ctttttgtca gaccctcgga tctgtgtctt gtttcatttt tcccaaaaca
     2761 cacttccatt tttgtaacca cttagtcaga ttgtaccata aacgttttca taagacaaat
     2821 gaatttgtat attttccaaa ttatttattt ttatttacat tcttttcata ataattggaa
     2881 caattttctc attttgtccc attgcaaaat tgcagcgttc acctcgcttc ttattttttg
     2941 aaaaaaactg cggttttatt ccacacatac tgccgttttt tctttccaac cggaaagctg
     3001 atagcttacc tcatagtgtc ccggctccag caggtatcta ccgttttctt ctgatccgag
     3061 cacttctgca accgcttctt tttcactttt ggcaaccatt ggaagtacgg cctctgcggt
     3121 acagtcgaac ggaacatcca tggtgatggt cacgtgctct ttgtcggaaa gctcccagcg
     3181 aatgctgtac attcccgatg cagaatcata ttttgcctct gcatacctca aatcccagtt
     3241 cagcgtcggt gcaaactgta cggtcctggc acccggatgg gattccatcg tgttgatgcc
     3301 ggcaacgtga cggaacatcc attcctggat ggagccgtaa gcataatggt tcatactgtt
     3361 catgctgatg ccggaaatcg taccgtccgt aagaagggaa ttccagcgtt cccagacggt
     3421 agttgcacca agcttaacct cataaagcca acccggaaat tcctcgttca gcaacagttc
     3481 gtaggcgaga tcgttcatgc cgttgtctgt cagcacgtta ttcagcagcg gtgttccgac
     3541 aaatcctgtt ttcagtttgt ggttactctg ctcaaacagc ttcagaagct gccgttttgt
     3601 cagctcttcg ttctttgaca gatggtattt cagcgtcagc agcagcgccg tctgtgtttt
     3661 gatgcagcag cggcctgttg cgctgtaata ttcttcctgt accacagcaa actgctcctc
     3721 ggacagcttc tgatacttct cctgctcctc gcggtatccc agcacaccgg ccgcttttgc
     3781 cacgattcct gcgctgatcg cataatacag gtttgcaata aactcctcgt ccgttgcacc
     3841 catgacctgc tccgctccct gtaccggatt gtccaacgca agccagtcgc cgaagtggaa
     3901 aatataccgc cagccgtggt tatccccatc cactttcgtg atgtaatcca cccacgattt
     3961 catgctcaca aactgatctt ccagaatact cttatccccg taaaacagat acagattcca
     4021 cggaatgatg cacgccgcat cgccccagac acacgctgca tcctcgacgc cgcacgacgg
     4081 caccacgtgc ggcacctttc cgccgagcac gctctgctct tttgccatgt cgtacaggta
     4141 ttttgcatag aatgcatacg tatcttcgag atacatcgcc gccggcgaga acacctgcgc
     4201 atcacccgtc catcccatgc gctcatcacg ctgcgggcag tcggtcggaa catctacaaa
     4261 gttgcacttt aatccgcagc gcacattact gatgagttgg ttgaccaaat cacttcctgt
     4321 tttcatccag cctgtcgcgg tgatattgct gtagtaagca agtccggtga aatcctcctt
     4381 tttcagatcc ggaataccct cgatttttac ataacggtat ccatagaagg taaaatgcgg
     4441 cacaagatct atttccgttc gatccgaaat ataaatgtat tcggattttg cactccgcaa
     4501 attttcattg tagaaattgc cgtgctgcag aatttctcct gtctgaacat gtacttttgt
     4561 tccttttggc acgttgacat ggagtttaaa gattcccgtg aattcctgtc ccatatcgaa
     4621 aaccagctca cctgccggag tatgaatcaa ttcttttggc cggaaagttt catgaaccgt
     4681 caccggaaga ctcatgcgat cggtcagttc ccctttcggt gcatcacaga ggactgcctg
     4741 ctctgcagga agatccggca gcgtatcatc ccgatgctcg ccgtcgtaga gatttgaaaa
     4801 cgaaattttg ctgcgtcgta tctgccatgt ctcatctgta ccgatgactt cttccgagcc
     4861 gtctgtatac ataagatgca gctccgcaat cagcttccag tcatttccgt aaaagcctct
     4921 atcctcaaac gcactgaaac caaaccgcgc cttataccag ccgttgccaa gcagtacacg
     4981 gagtgttccc tcgctcgaaa actcctctgt cacatcgtat gtctgatact gtacccactc
     5041 attgtaatca ttgctgtacg gtgtcagata ttcatttccg acacgcttct catcaacaaa
     5101 cacttcatac agtccaagtc cgcatatata cagccgtgct tttgccactt cttttgccgg
     5161 aataattttc ttttcaaaat aaggatggcg tttttcttca ctgctgcagg agacccattt
     5221 ggcagaccat gcttcctccc gctttccagt ttcaaaaaac tgcacttcgc ttactgcttt
     5281 ttctccggca tcgctgcctg cttccaccgt ccagtaatac cgtgttctcg gctccaactt
     5341 cagtttcacc ggatagcaaa ggctactcgc tttcgtatcc tcccccgagt caaacaacag
     5401 atgttccatc gcctcatccg atgatacacg gatccttgca taactctgct gctttccttt
     5461 cgcttctttt attttccacg aaaagaccgt ccgttccatc cgaaatccca ccggattttc
     5521 cagatggttc acttgtgcgt gataaatctt catattaatt cctcctccat ttttgtattt
     5581 ca
//
LOCUS       DAOMUF010000141         5525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4052_length_5525_cov_4.532176, whole genome shotgun sequence.
ACCESSION   DAOMUF010000141 DAOMUF010000000
VERSION     DAOMUF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4052_length_5525_cov_4.532176"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLN77_08140"
     CDS             <1..291
                     /locus_tag="PLN77_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRM44277.1"
                     /translation="TERGESGKTEKRRGNAKKGCDSVRKETENMVENSRAKLEKKHLD
                     MIAANNLKTEDAGFGVATNVVTLITKDGIKELPLMGKDEVAGCLLDEIAARR"
     gene            497..1267
                     /locus_tag="PLN77_08145"
     CDS             497..1267
                     /locus_tag="PLN77_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRM44278.1"
                     /translation="MIFETHAHYDDEKFDEDRAELLSSMQENGIGRIINVSANLESLE
                     NTRKLMEAYPFIYGAFGLHPDEVGDLNEDVMARMRELCRMEKAVAVGEIGLDYYWDKE
                     NHEKQEYWFRRQIALAREEKLPMIIHSREAAADTLRVMKEEKSEEIGGVIHCFSYSVE
                     MAEEYLKMGFYLGIGGVVTFKNAKKIKEVVQMAPMERILLETDSPYLAPVPYRGKRNS
                     SLYLPYVVQEIAELKGISEEEVIETTEKNAVRLFRAEE"
     gene            1353..3356
                     /locus_tag="PLN77_08150"
     CDS             1353..3356
                     /locus_tag="PLN77_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HRM44279.1"
                     /translation="MVDKTMMGDKCAELVRAAMIGALAVTLGNPTAVYAAEEETADPG
                     QIGIEIAGEAAVGAASFCVEEDAAEEAPAEAEEVTEPEGTQNDEMEEESGSEGKEDET
                     VPEEPEPQEPDGGENAGMTEPGKTGETEPEGPENAEPGEAGETEPEEPENPEPGDQGD
                     MPETPGNSEMDGPWPGEGEDISNDPWPGQEEIGGGDGEQMEPPEEENGSAGSDDAAGG
                     DKTAEVSSDSDLSQSAEPPAAEAEPVFSAEQEAERTRESYSEWQAAEAEKVTISRMPW
                     LFHTVEKTYAIADVNSWLHVREGKGTNQKIVGILPKGSLCYILADADSDWVYVESGDV
                     RGFVCARYLLRGEEAEKEVNRWQEESFPMAEMWVKPWNNKAYTYTYATTRTLLSSDEA
                     RGEVLQYAKKFLGNPYVWGGTSLTNGCDCSGFAQQIFANFGYTLPRTSRQQAKAGTRI
                     PVQEAKPGDLLFYQRESGFIYHVMIYLGDGKVIHAGSEATGILISDFNYEKSTEFAVR
                     VISEEIRESKIETGDVDNGRKEGNSVDNQTTENGNGGKQKAGAENVQNTSAENSTAGD
                     RLESASGKYLGNFKLTAYCNCAVCCGRWAGGPTASGKMPVQGRTIATGVLPFGTKLNI
                     GGKIYTVEDRGTPYGHIDIYMERHADAEEFGVRYADVYQSEEI"
     gene            complement(3338..4186)
                     /locus_tag="PLN77_08155"
     CDS             complement(3338..4186)
                     /locus_tag="PLN77_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRM44280.1"
                     /translation="MSHKIGITACSNPMHPSFSADLARLCQILRELGFDPVLSPCLFD
                     NGSGFSGTGAERADALMNLYCDPEITDIFDVSGGDMANEVLPYLDFSVIAASGKRFWG
                     YSDLTTIVNAVTTATGNESMLYQVRNLLYDHSAEQITLFQNAFSGQSPSLFDLPCTFL
                     QGDHMEGVMIGGNIRCFLKLAGTDFQPDFRRKILLLEAMGGGVPQMVTFLSQLKMMHA
                     FEQINGILLGTFSQMERDQLTPDMPQLVRQAAGPDLPIAKTPYIGHGTDAHAAVIGKF
                     YQISSD"
     gene            complement(4319..5353)
                     /locus_tag="PLN77_08160"
     CDS             complement(4319..5353)
                     /locus_tag="PLN77_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM44281.1"
                     /translation="MIRFNNDYNHGAHPEILEALGAINGNSYGGYGEDEWCEKAAELI
                     REKIRCADAAVHFFPGATQANFIVTAAALSPVQSVIAADTGHINCHEAASIENTGHKI
                     LELPNTDGKISAEQIAACAAAYYEGGEPEYLTEPKMVYLSFPTEQGTLYSLQELTKIH
                     EVCRKYDMYLFVDGARLGYGLGSEKNDVSLEDLAALTDVFYFGGTKCGALFGEAVVLT
                     ADSLKKRFKAYMKQNGAVLAKGWLLGLQFHCLLGHDLYFTATRRADELAMKLRKAFAA
                     QGIPFWVESFTNQQFVILTDAQKETLEKRYIFEPMGKSADGGNIARFCTSWATTEEEV
                     QTLIDDLKAL"
BASE COUNT         1524 a         1277 c         1577 g         1147 t
ORIGIN      
        1 actgaaagag gagaatcagg aaaaactgaa aagcggagag gcaacgcgaa gaagggctgc
       61 gattccgtaa gaaaagaaac ggagaacatg gtggaaaact cgcgtgcgaa gctggagaag
      121 aagcacctcg acatgattgc ggcaaacaac ctgaagacgg aggacgccgg cttcggtgtg
      181 gcgacgaatg tggtgacgct gatcacgaag gacggcatca aagagcttcc gctgatggga
      241 aaagacgaag tggctggctg cctgctggac gagattgcgg cacggcggtg attttatcgg
      301 aatccggcat gttttttcgt ttcctggttg acgcgggaaa aatgaatgcg ttataataag
      361 cacaggcgca gcggcgcact ttacttttac gaaaacaacg aacgatagga tggataaacg
      421 tggaaacgga aacaggggaa ggctatgcag agctttcttc cgtttctgtt tcgttttttt
      481 aaggagaaat tcggatatga tatttgaaac acatgcacat tatgacgatg agaaatttga
      541 tgaagaccgc gcagagctgc tttcctcgat gcaggaaaac gggatcgggc gtatcatcaa
      601 tgtctcggca aatctggaat cactggaaaa tacaagaaaa ctgatggaag cgtatccgtt
      661 catctacggc gcgttcggcc ttcacccgga tgaggtgggc gatctgaacg aggacgtgat
      721 ggcgcggatg cgggaactct gccgcatgga aaaggcggtc gcggtcggtg agattggcct
      781 tgactattac tgggacaagg agaatcacga gaagcaggaa tactggttcc gccgtcagat
      841 tgcgcttgct cgggaagaaa aacttccgat gattattcac agcagggagg cggcggccga
      901 tacgctccgc gtcatgaaag aagaaaaatc ggaggagatc ggcggcgtca ttcattgttt
      961 ttcctactct gtggagatgg cagaggaata cctgaaaatg ggattttatc ttggcattgg
     1021 cggcgtggtg acgttcaaaa atgcgaagaa gatcaaagaa gtggttcaga tggctccgat
     1081 ggagcgcatt ctgctggaaa cagacagtcc gtatctggca ccggttccgt accggggaaa
     1141 acgaaattct tctctgtatc tgccgtacgt ggtgcaggag atcgcagaac tcaagggaat
     1201 ttcggaagaa gaagtcattg agacgacaga aaaaaatgcg gtcagactgt ttcgagcaga
     1261 ggaataggaa gcgataccag aggtgtttct tctgttttat ttgttcggat caattccgtc
     1321 tggccggaca aattgaatag gagaaagcga aaatggttga taaaacaatg atgggcgaca
     1381 aatgcgccga gctggtgcgc gccgcgatga tcggtgcgct cgcagtgaca ctgggaaacc
     1441 cgacagccgt ttatgcagca gaagaggaaa cggccgatcc gggacagatt ggaattgaga
     1501 ttgcagggga agcagccgtt ggggctgctt ctttttgcgt ggaggaagat gcggcggagg
     1561 aggcacctgc ggaagcggaa gaggtgacag aaccggaagg tacgcagaat gatgagatgg
     1621 aagaggaaag tggatccgag ggaaaagaag atgagacagt cccggaggaa ccggaaccgc
     1681 aggagcctga cggcggagag aatgcaggaa tgacggaacc gggaaaaacg ggcgagacag
     1741 agccagaggg gccggaaaat gcagaaccgg gagaggcagg cgagacagag ccagaggagc
     1801 cggaaaatcc agaaccggga gatcagggag acatgccgga aacaccggga aacagtgaaa
     1861 tggatggacc gtggccgggc gaaggggaag atatttccaa cgatccatgg ccggggcagg
     1921 aagaaatcgg cggcggagat ggggagcaga tggaaccgcc ggaggaagaa aacggatctg
     1981 caggaagtga tgatgcagct ggtggggaca agacggcaga agtctcttcg gattctgatt
     2041 tatcacagtc tgcggaaccg ccagccgccg aggctgagcc cgtcttttcg gcagagcagg
     2101 aagctgagcg cacgcgggaa agctacagtg agtggcaggc ggcagaagct gaaaaagtca
     2161 cgatttcccg tatgccgtgg ctgtttcata cggttgaaaa aacatatgct atcgccgatg
     2221 tgaattcctg gcttcacgtc cgcgaaggaa aaggcacaaa tcagaaaatc gtcggaatcc
     2281 tgccgaaggg aagcctctgt tatattttag cggatgcgga ttctgactgg gtttatgtgg
     2341 aatccggcga tgtccgtgga tttgtctgcg cgcggtatct cctgcgcgga gaagaggctg
     2401 aaaaagaggt aaaccgttgg caggaggaaa gttttcccat ggcggaaatg tgggtgaagc
     2461 catggaataa taaggcgtac acctatacat atgcaacgac gagaacactt ctttcttctg
     2521 acgaagcgcg cggcgaggtc ctgcagtacg cgaaaaaatt cctgggaaat ccgtacgtct
     2581 ggggcgggac gagtcttaca aacggctgcg actgttccgg ctttgcccag cagatttttg
     2641 ctaatttcgg gtataccctt ccgagaacct cacgccagca ggcgaaagct ggaacacgca
     2701 ttccggtgca ggaggcaaag cctggtgatc tgctgtttta ccagcgggag agcgggttta
     2761 tttaccatgt catgatttat ctcggtgatg gaaaagtaat ccacgctgga agcgaggcga
     2821 cggggattct gatttcagat tttaactatg agaaatcgac agaatttgct gtgcgggtga
     2881 tttcggagga aatacgtgag agcaaaatag aaacgggaga tgtggataat gggagaaaag
     2941 aaggaaactc tgtggataac cagacaacag aaaacgggaa tggtggaaaa cagaaggcgg
     3001 gagcagaaaa tgtgcagaat acaagtgcag aaaacagtac cgcaggtgat cggttggaaa
     3061 gcgcttcggg aaaatatctc ggcaatttta aactgaccgc ttactgcaac tgcgccgtct
     3121 gctgtggccg ctgggcgggc ggcccgaccg catccggaaa aatgccggtg cagggccgga
     3181 ccattgcaac cggcgttctg ccgtttggaa cgaagctgaa tatcggcgga aaaatttaca
     3241 cggtcgagga ccgtggcacg ccgtacggcc acattgatat ctatatggaa cgccatgcag
     3301 acgcagagga gttcggcgtg cgctacgcgg atgtatatca gtcagaagaa atctgataaa
     3361 atttgccgat cacggcggca tgtgcatccg tgccgtggcc gatatacgga gtctttgcaa
     3421 tcgggagatc cggtccggcc gcctggcgga caagctgcgg catatccggc gtcagctggt
     3481 cgcgttccat ctgggaaaat gttccgagga gaataccatt gatctgttca aaggcatgca
     3541 tcattttcag ctgggacaga aacgtaacca tctgcggaac gccgccgccc atggcttcga
     3601 ggagcaggat ttttctgcgg aaatccggct gaaaatccgt tccggcgagc ttcagaaaac
     3661 agcggatatt tccgccgatc attacacctt ccatgtgatc gccctgcaga aatgtgcaag
     3721 ggaggtcgaa gaggctggga gactgcccgg aaaaagcgtt ttggaacaga gtgatctgtt
     3781 cggcgctgtg gtcgtacagc agatttcgaa cctgatacag catcgactca tttccagtcg
     3841 cagtcgtgac agcattgaca atcgtcgtga gatcactgta tccccagaag cgcttgccgg
     3901 aggcggcgat gacagaaaaa tccagatacg gcagaacttc attcgccata tcgccgccgg
     3961 aaacatcaaa aatatccgtg atttctgggt cacagtaaag gttcatcaac gcatccgccc
     4021 gctccgcgcc cgtgccggaa aagccggaac cgttgtcgaa gaggcagggg cttaaaacag
     4081 gatcgaagcc cagctcccgc agaatctgac agagacgcgc cagatcggcg gaaaaggaag
     4141 gatgcatggg gttggagcag gcggtaatac caattttatg agacataaag attcctttct
     4201 cgaagtaaaa caaggcaaaa tctgcatttt cgtggcacac cggtacagct ttttctttga
     4261 atttgcaaag cagatttaga gatcgccggt gtttgcagaa tgccgttttg gacaaaaatt
     4321 ataaagcttt gaggtcatca atcagtgtct gcacttcctc ttccgtcgtt gcccagctgg
     4381 tgcagaaccg cgcaatattt ccgccgtccg cggatttccc cattggctcg aaaatatagc
     4441 gtttttccag agtttccttc tgcgcatcgg tgagaatcac aaactgctga ttcgtaaaac
     4501 tctccaccca gaatggaatc ccctgcgccg caaatgcttt gcggagtttc atggcaagtt
     4561 catcggcgcg gcgtgtcgca gtgaaataaa gatcatgccc cagcaggcag tggaactgca
     4621 gtccgagcag ccatcccttt gcgaggacag cgccgttctg tttcatgtag gctttgaacc
     4681 gctttttcag cgagtcagct gtaagcacaa cggcctcacc aaacagcgca ccgcatttcg
     4741 ttccgccgaa atagaaaaca tctgtgagag cagcgagatc ctcgagagaa acatcattct
     4801 tctctgatcc aagcccgtat ccaagccgtg ccccgtccac aaacagatac atatcatact
     4861 tccggcagac ctcgtgaatc ttcgtcaact cctgcagcga atacagcgtt ccctgctcag
     4921 tcggaaatga aagatatacc atcttcggct ccgtcagata ctccggctca ccgccttcat
     4981 aataagccgc cgcacacgcc gcaatctgtt ccgccgaaat tttaccatcc gtattcggca
     5041 gctccagaat cttatgcccg gtattttcaa tcgaagccgc ctcatggcag ttaatatgtc
     5101 cggtatccgc cgcaatcaca ctctgcaccg gcgaaagcgc tgccgccgtc acaataaaat
     5161 tcgcctgcgt cgcccccgga aaaaaatgaa ccgccgcatc cgcacaacga atcttctccc
     5221 gaatcagctc cgcagccttc tcacaccatt catcctcccc atatccacca taactgttcc
     5281 cattaatagc ccccagcgcc tccagaatct ccgggtgcgc accgtgattg tagtcattat
     5341 taaatcgtat catcagttta tgttctcctt aatttgaatt tgaaaaatgc tgtttccatt
     5401 atacggcatc cgaaggaaaa cagcaattgg agaacaaaaa gagaaaatgc aaaaaaatgc
     5461 agaaggagga ttatctgcat cgtacaatat tggagcaaga tattttatcc gtgaaatttt
     5521 aaaac
//
LOCUS       DAOMUF010000142         5513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4063_length_5513_cov_3.894650, whole genome shotgun sequence.
ACCESSION   DAOMUF010000142 DAOMUF010000000
VERSION     DAOMUF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4063_length_5513_cov_3.894650"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLN77_08165"
     CDS             <1..482
                     /locus_tag="PLN77_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44282.1"
                     /translation="KTMKHRIKILLLAVLIAALCNCMLMIGMTKTKEKNTIPKDASLI
                     CEETVSPNKDYITDEKDLVNYTVRVYQEKNNDIIVYAESNSPLFDEAQYIVICDHAIT
                     ADDVHIKWTTLMGSTEASEKDEIGLAHVSISKDGEVFSEKTISFVAKAVNAVTDVIG"
     gene            complement(610..1767)
                     /locus_tag="PLN77_08170"
     CDS             complement(610..1767)
                     /locus_tag="PLN77_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM44283.1"
                     /translation="MDKRTEQAFAYLEKNEHSMMELLKNLVSIETPSADREANERIAA
                     HLDTYCDALGMERQIHHFEKAGPTFAAWTRPQEKKPILLLGHMDTVHAVGKFGPEPFL
                     VKDDRVYGPGVYDMKGGDVIALFVLRALNAVGYEDRQIKLVLTGDEEVAHAFSHGEAG
                     KLVEQYASGCEAAFNLESGYTSGEVTTRRNGGAIIRVKVKGKAAHAGKEPQKGASAIL
                     QAARMITEIESRSVFGYLSFNCGRISGGTGANVIPDSCEFSIGIRYAANAQYEEAIAF
                     LKNLCEHVTVPGTSCTMEQNGYYPAMEMVDGADRLLSLYQESCKLLGEPIPQGIQQSG
                     CSDTSFVTRIGIPALCSLGIQGAGAHSLDEYAIPSSLLVQCKKLVATILAM"
     gene            complement(1791..3077)
                     /locus_tag="PLN77_08175"
     CDS             complement(1791..3077)
                     /locus_tag="PLN77_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44284.1"
                     /translation="MKIGVTGSDHICDRIQKTLEKRMPDLEVVYRRSNDYRYGLEAAA
                     QFQKGKVSGIIFTGPTNYHYALKRLEPNVPWTFLPHNQASILKALAESAVRYSCVPDV
                     ISIDMYEEQLVQETLEEIGISNGRVLVAHGSSPDQGDFQDALTDFHRYNYFHNGAKIC
                     FTNMEKTYEALSAEGIPCIRISVSEDVILEQVYHLQFLENTAQKNRGQSASVQIYFDY
                     SFDQETDLSLREWEKVHYQNEMRELIYSAAHRMGAAAFSEGASIFYIMSSRPVLMREF
                     IQNGEYQKILFHGQQTPHNRLWIGIGYGDTPMEAKSRAAMALNHSIADRSGANYIAED
                     EHQFTEIKINDPTDHTAYLLHRLHISSSTYEKLKEILRSHDQVITSAQLAADMGITER
                     SANRLILRLEQENCVTTIGKISGGRGRPARIMKITL"
     gene            3235..4332
                     /locus_tag="PLN77_08180"
     CDS             3235..4332
                     /locus_tag="PLN77_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM44285.1"
                     /translation="MLDELTELMRIDVSSGQETEIAEHLVKKLEQMGFAITRDEAGAT
                     FGGVCGNILAVREGERDGVVCFCSHMDRVPGGIGIKPVEEDGILRSSGDTILAADDLS
                     GVAAILEGVRQAIESGKALPKLEILFTVGEEAGLYGAKAFDVSLLTSKTMYVLDSPGR
                     IGRIVNGAPGRALLEAQVFGKAAHAGNEPEKGIDAAKIMAEMLATMETGRLDENTTSN
                     FSHLWSKTCEAMNVVCDHAILRGEMRSRSGEKLDRYEAYFQSHCREIAEKRGARVEFK
                     RENTFLPFFIPEDSEMIQIVTESLKEMGIEPQINGGGGGMDGNIFNQKGITCVGIATG
                     YFKNHSTEEYLHLDDFYHAGELVARIIGRIA"
     gene            4428..>5513
                     /locus_tag="PLN77_08185"
     CDS             4428..>5513
                     /locus_tag="PLN77_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRM44286.1"
                     /translation="MAEILTNNAMFSSFMIVLIVFALGDIVGKITKGKLSGMMVVMLL
                     FLVGFLTKLIPADIIDQGGLTALSKLAIAMVLFNMGTTLNVKQLIEEWRTVLMAALCM
                     LASCLVMLLVSPIIGFDTVLVGMPVINGAAMATSLMASAAAEKGLATAAALCAVIYSV
                     QKFVGAPIASAMGIRYGKKLLKAYRENPTQFQKQETGKGASAKVFFADKHKEWYSANV
                     MMALVAAGSWVAHILGDLTPINYSIWALLLGVVCVASGLVPTKPLQKSNSYGLMMIAV
                     FGSIIPSLAKVSLSDLGTMAFQTIVLFAAALIGVALVGWVLPTWKLVGDKDLAVGIGV
                     EQFLGFPSNVVICREVGDAVGETPEEKA"
BASE COUNT         1396 a         1355 c         1469 g         1293 t
ORIGIN      
        1 agaaaacaat gaaacataga ataaaaatcc tgctgcttgc agttctgatc gcagcgctct
       61 gcaactgtat gctgatgatc gggatgacca aaacgaaaga gaagaatacc attccaaaag
      121 atgcaagcct gatctgcgaa gaaaccgtat cgccgaacaa ggactatatt acggatgaaa
      181 aagacctggt aaattatacc gtgcgcgttt atcaggaaaa aaacaacgac atcatcgtct
      241 atgcggaatc caattcgccg ctgttcgatg aagcacaata catcgtaata tgcgaccacg
      301 ccatcaccgc cgacgatgtc catatcaaat ggaccaccct catgggcagc accgaagcga
      361 gcgagaaaga tgagatcgga ctcgcacacg tttcgatctc aaaagacggg gaagtgttca
      421 gcgaaaagac gatcagcttt gtggcgaaag cggtgaatgc ggttacggat gtgatagggt
      481 aaagaaaata agataataaa aaagaaacga gcaactccct cggctcacga tgagcttacc
      541 ggacaaaaaa cccgtttctt tttttatgct aaaatgactt tcctgttttg tcaagaaccg
      601 catagcattt tacatcgcca aaatcgtggc caccagtttc ttgcactgca cgagcagtga
      661 ggacggaatc gcatactcat caagtgaatg tgcgcctgcg ccctggatac cgaggctgca
      721 gagggcgggg attccgatgc gggtgacgaa ggaggtgtcg gagcagccgc tctgctggat
      781 gccctgcggg attggttcgc cgaggagttt acagctttcc tgatagaggg agaggaggcg
      841 gtcggcaccg tcgaccattt ccatggcggg gtagtagccg ttctgttcca tggtgcagct
      901 tgtgccagga acggtgacgt gttcgcagag attttttaag aaagcgatgg cttcttcata
      961 ctgcgcattt gccgcgtatc ggataccgat ggaaaactca caggaatccg ggatgacatt
     1021 ggctccggtt ccgcctgaaa tacggccgca gttgaaggaa agatagccga acaccgagcg
     1081 gctctcaatc tctgtaatca tgcgcgccgc ctgcagaatc gcactcgcgc ctttctgcgg
     1141 ctcctttcct gcatgagccg ctttgccctt tactttcacg cgaatgatcg cgccgccgtt
     1201 tcttctcgta gtcacttcgc cgctggtgta gcccgattcc agattaaaag ccgcctcaca
     1261 gccgctggca tactgctcga caagctttcc ggcctcgccg tgggaaaatg catgggcgac
     1321 ttcctcatca ccggtgagca ccagtttgat ctgtctgtcc tcatacccga ccgcattcag
     1381 tgcgcggagc acaaacagcg cgatcacatc gccgcccttc atgtcataaa cgcctggacc
     1441 gtacacgcgg tcatccttca caagaaacgg ttccgggccg aactttccaa ccgcatgaac
     1501 tgtatccata tgcccaagca gaagaatcgg cttcttttcc tgcggccggg tccacgcggc
     1561 aaacgtcggg ccagccttct caaagtgatg aatctgacgc tccatgccga gcgcatcaca
     1621 gtacgtatcg agatgcgccg caatccgctc attcgcctcg cgatcagccg aaggtgtttc
     1681 aatggagaca agatttttca gcagctccat catactgtgt tcgtttttct cgagataggc
     1741 aaatgcctgt tccgttcttt tatccataaa cattctccat ttcttatgtt ttataaagtg
     1801 attttcataa tccgtgccgg ccgtcccctg ccgccgctga ttttgccgat cgtggtcaca
     1861 cagttttcct gttccagccg cagaatcagc cggttggcgc tccgctccgt gatccccata
     1921 tctgcagcga gctgtgcgga agtgattacc tgatcatgtg accgcagaat ctctttcagt
     1981 ttttcatacg tactgctgct gatgtgaagc cggtgcagaa gatacgccgt gtgatcggtc
     2041 gggtcattga tcttaatttc ggtaaactga tgctcatcct ccgcaatata gttcgcgccg
     2101 gagcggtccg cgatcgagtg gttgagcgcc attgccgcgc gggatttcgc ctccatggga
     2161 gtatcaccgt agccgatgcc gatccacagt cggttgtgcg gcgtctgctg tccgtgaaac
     2221 agaatcttct gatattcccc gttctgaata aattcccgca tcagaaccgg acgggatgac
     2281 atgatataaa aaatagaagc gccctcggag aacgcagcag cccccatgcg gtgggcggca
     2341 gagtagatca gctcgcgcat ctcattctga taatgaacct tctcccattc ccggagcgaa
     2401 agatccgtct cctgatcaaa ggaataatca aaatagatct gcacggaagc actctgcccg
     2461 cggttcttct gcgccgtatt ttccagaaac tgaagatgat aaacctgttc cagaattaca
     2521 tcctcggaaa ccgagatgcg gatgcaggga atgccttctg ccgacagtgc ctcatacgtt
     2581 ttttccatgt tggtaaagca gatttttgcc ccgttgtgaa aatagttgta acggtgaaaa
     2641 tccgtgaggg catcctgaaa atcgccctga tcgggagaag agccgtgtgc cacaaggacg
     2701 cggccattgg aaatgccgat ctcttccagc gtttcctgca caagctgttc ctcatacata
     2761 tcgatactga tgacatccgg cacgcaggaa taacggacag cagattctgc cagcgctttt
     2821 aagatggaag cctgattgtg cggaagaaag gtccagggaa cgtttggctc cagccgcttt
     2881 aaagcatagt gatagttggt cggcccagta aaaataatgc cggatacttt ccctttctga
     2941 aactgggcag ccgcttccag accgtaccgg taatcgttgg aacgccggta gaccacttca
     3001 aggtccggca tgcgcttctc cagcgttttc tgaatcctgt cgcagatatg gtcagaacct
     3061 gttacgccga ttttcatata taaaattcct ttccgagaat taaagacata aaaatgaatt
     3121 atggcataat tttacaccta ttaaggacga aaggcaaatg gtaagatgta gaagaattgt
     3181 ttttgaaaat ttttttcaag aatggagagt ggaaaagaaa tgaaaaatca acgtatgtta
     3241 gatgaactga ccgaattgat gcggattgat gtgtcgagcg gtcaggagac ggagattgca
     3301 gagcatctgg tgaaaaaact ggagcagatg ggatttgcga ttacgcggga tgaggccggg
     3361 gctacgtttg gcggcgtgtg cggcaatatt ctggcggttc gggaaggaga gcgggacggt
     3421 gttgtctgtt tctgttccca tatggaccgt gtgccgggcg gcattggaat caaaccggtg
     3481 gaggaggatg gaatccttcg ttcttcgggc gacacgattc tggcggcgga tgatctgtcc
     3541 ggtgtggcag ccattttaga gggcgtgcgt caggcgattg aatccgggaa ggcgcttccg
     3601 aagctggaga tcctctttac ggtcggcgag gaggccggtc tgtacggtgc gaaagcattc
     3661 gatgtaagcc tgctgacatc aaaaacgatg tatgtgctcg actccccggg acgcatcggc
     3721 cgaattgtaa atggtgcgcc gggccgtgcc cttctggaag cacaggtttt cggcaaggca
     3781 gcccatgcgg gaaatgagcc ggagaaaggc attgatgcgg caaaaattat ggcggaaatg
     3841 ctcgcaacga tggagacggg aagactggat gaaaatacaa cgtcgaactt ttctcacctc
     3901 tggtccaaaa catgcgaggc aatgaacgtt gtctgcgacc acgcaatcct gcgcggagag
     3961 atgcggagcc ggtcgggaga aaaactcgac cgctatgaag cgtatttcca gtcgcactgc
     4021 cgtgaaatcg cagaaaagcg cggtgcgcgt gtggaattta aaagagaaaa caccttcctg
     4081 ccgttcttca tcccggagga ttccgagatg attcagatcg tcacggaatc cctgaaagag
     4141 atgggaatcg aaccgcagat caacggcgga ggcggcggta tggacggcaa tattttcaat
     4201 cagaagggaa tcacctgtgt cggtatcgca acaggatatt tcaagaatca ttcgaccgaa
     4261 gaatatctgc atctcgatga cttctatcat gccggagagc ttgtggcgcg gataattgga
     4321 aggattgcat aaatatgcaa aatataaaat aaaaactgga taaaacttgc aaaataaaag
     4381 aaaattatgt ataatttcaa attaaaaaga gcaagggaga aaaagaaatg gctgagattt
     4441 taacaaacaa cgcaatgttc tccagcttca tgattgttct gatcgtgttt gcactcggcg
     4501 acattgtcgg taaaattaca aaaggaaagc tgtccggaat gatggtcgtc atgctcctgt
     4561 tcctggtagg ctttctgacg aaactgatcc cggcggacat catcgatcag ggaggcctta
     4621 cggcgttatc aaaactggca attgcgatgg tactgttcaa catgggtact accctgaacg
     4681 tgaaacagct gatcgaggag tggagaaccg tattaatggc ggcactctgt atgctggcgt
     4741 cctgccttgt gatgcttctg gtatccccga tcatcggatt tgacactgtg ctcgtcggca
     4801 tgccggttat caacggtgcg gcaatggcga cttcgctgat ggcaagcgcc gcagcagaaa
     4861 aagggctggc aacggcagca gcactctgtg cagtcatcta ttccgtacag aaattcgtcg
     4921 gtgcgccgat cgcatctgcg atgggtatcc gctatggaaa gaaactgctg aaagcgtacc
     4981 gcgagaatcc gacacagttc caaaagcagg agaccggaaa gggtgcttcc gcaaaagttt
     5041 tctttgcaga caaacataaa gaatggtatt ctgccaacgt catgatggca ctcgtggcag
     5101 ccggttcctg ggttgcccat attcttggcg atctgacccc gatcaactac agcatctggg
     5161 ctctgcttct cggcgttgtc tgtgttgctt caggactcgt tccgacgaaa ccgctgcaga
     5221 aatcgaactc ctacggcctc atgatgattg ctgtcttcgg ttccatcatc ccgtccctgg
     5281 cgaaagtttc cttaagcgat ctcggcacca tggcattcca gacaatcgtt ctgttcgccg
     5341 cagcgctgat cggtgttgca ctggttggat gggttctccc gacctggaaa cttgtcggcg
     5401 acaaggatct ggctgtcggc atcggtgtcg agcagttcct cggattcccg agcaacgtag
     5461 tcatctgccg cgaggtcggc gatgcggtcg gagagacacc ggaggagaaa gcg
//
LOCUS       DAOMUF010000143         5509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4068_length_5509_cov_4.545288, whole genome shotgun sequence.
ACCESSION   DAOMUF010000143 DAOMUF010000000
VERSION     DAOMUF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4068_length_5509_cov_4.545288"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLN77_08190"
     CDS             complement(<1..379)
                     /locus_tag="PLN77_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM44287.1"
                     /translation="MEEISPNNILKVSHISASYQEGKQRKTVLHDISFELHENEILGL
                     VGESGCGKSTLSKVILGFIRPDEGEIISQVDHPQMIFQDPFSSLNPAKKISWILEEPL
                     RMQKVPKEERKKRVLAMAERVGLS"
     gene            complement(383..1261)
                     /locus_tag="PLN77_08195"
     CDS             complement(383..1261)
                     /locus_tag="PLN77_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44288.1"
                     /translation="MALLDVRDLKIAFYDTTPPTEVVKGISFSLEEGETLGIVGESGS
                     GKTQTALSILHLLKENSAVSGGEILYEDSSVAHASSQDLQRIRGKEISMIFQEPMTSL
                     NPVLTIEEQIAEGLLIHESCTPAERHEKVLAIMHEVELPDPEKLCKKYPHQLSGGQRQ
                     RVMIAAALITEPKLLIADEPTTALDVTIQSQILKLLKKINERHHTAILFISHDLGVIR
                     SLCDRVIVMNHGTIVESGSVDDVFFHPKEDYTRRLLDAIPNRRTSLRKRIKIAPTAQW
                     PTGSDKKAAPPAEKEE"
     gene            complement(1263..2066)
                     /locus_tag="PLN77_08200"
     CDS             complement(1263..2066)
                     /locus_tag="PLN77_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM44289.1"
                     /translation="MKKKRNLTLIIGAVLTAFMLLFLLIGFFYTPYDPDAMNGAAKFA
                     APSLSHPFGCDHFGRDIFSRVLKGTGTTFFVAFATLLLGGTIGVLVGAFTGYFGGWID
                     EILMRINDVIVSFPGILLALVFIALLGPGKYNVILALGIVFIPSFARITRSEFLARKD
                     MDYVKSARLMGVSHLRIIFVHILPNTVPSLLSMAAIGFNNAVLSEAGMSFLGIGVQPP
                     DASLGRMLSESQTYLMTAPWGSVFPGLAVILLALGVSLLGDGLQKKGGN"
     gene            complement(2145..3089)
                     /locus_tag="PLN77_08205"
     CDS             complement(2145..3089)
                     /locus_tag="PLN77_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM44290.1"
                     /translation="MKYIAKKFGSLLLTLLLVSFLSFFAFSVIPGDAAKSQLGTEATP
                     ERIEALQKEMGLDRPVLERYASWVTGFFHGDLGTSYSYSMPVGELIRDKVPITIAITV
                     IAFLMILVVSIPIGIFTAQHAGGHLDRLIMTINQVIMSVPGFFIGILITYLFGLILKW
                     FQPGAYVPISENFSGFIGYLVAPSAAIALPRCSMAVKMLRSSVLSQLDADYVRTAYSR
                     GNSTRRVMYHHVLKNALIPVLTFWGMTIADIVANSIILEQVFSIPGMGNLLITSISNR
                     DYPVVQGILVLIAALIIIINFVVDLLYGRIDPRIRVKD"
     gene            complement(3347..3898)
                     /locus_tag="PLN77_08210"
     CDS             complement(3347..3898)
                     /locus_tag="PLN77_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HRM44291.1"
                     /translation="MKATGIVRRIDDLGRVVVPKEIRRTMRIREGQAMEIYTGREGEI
                     ILKKYSPIEELAECAAGFTKSISTVTGCLVCVSDQEQIVAANGPEQKNYLGKSISSRL
                     EEAILNRKTVLARPGEKAYFSILENTDNTTPLPELIFPILSSGDAIGSVILCGKDRES
                     LPGDTEKKLLQVAATYLGREMEN"
     gene            complement(4070..4963)
                     /locus_tag="PLN77_08215"
     CDS             complement(4070..4963)
                     /locus_tag="PLN77_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM44292.1"
                     /translation="MFQVFVDSAANLPAVTAKKYDIHVVSFVNLVNGKELVCFDPDLS
                     PEEELAKGKEYYNAMRTGCEIKTGLISTANFADAFRTALEADQDVIYLSLSKNISGTY
                     NSARLAAEELLEEYGGKRKIRLVDSLNASLAQGILAIYASEMRDRGMNVDEAADLLET
                     YVSKMNGVFTVGDLKYLSRTGRIKGSVAVVGNMLKIKPILRGSEDGYIVSYKNVRGRK
                     AVLNELIRLFCETIEEPEKQIVGIAHADAYEESLYMMEEIQKRVKVREFINTSYDYCT
                     GSHVGPDTIALFYLGKDRSLL"
     gene            5320..>5509
                     /locus_tag="PLN77_08220"
     CDS             5320..>5509
                     /locus_tag="PLN77_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44293.1"
                     /translation="MHEYEIFIEEINPCGGEKHSKKTLIEAEAVSPEAYVKENGRFPV
                     LESMCNESGDVVIVTGDNK"
BASE COUNT         1483 a         1279 c         1365 g         1382 t
ORIGIN      
        1 cggagaggcc gactctttcg gccatcgcaa gaacccgttt ttttcgttct tcttttggaa
       61 ctttctgcat ccgaagcggc tcctctaaaa tccaggagat tttcttcgca ggattcaggg
      121 aagaaaacgg atcctggaaa atcatctgcg gatggtcgac ctgcgaaatt atttcgcctt
      181 catctgggcg gataaatcct aagatcactt tggagagtgt cgattttccg catccgctct
      241 ctccgacgag accgagaatt tcattttcat gaagctcgaa agagatatca tgaagcaccg
      301 tttttctctg ctttccctcc tgataggagg cagaaatatg gctgactttc agaatgttgt
      361 tcggactgat ttcttccatg ggttattcct ccttttccgc cggcggagca gcttttttgt
      421 cagagcctgt cggccattga gctgttggag cgattttgat tcgctttcgc agcgatgttc
      481 gtcggttcgg aattgcatcg agcaggcggc gggtgtagtc ttccttcggg tggaaaaaga
      541 catcgtctac actgccggat tccacaatcg tcccatgatt catgacgatg acccggtcac
      601 agagcgaacg gatgacgccg aggtcgtgtg agataaaaag aatcgccgtg tgatggcgtt
      661 cattgatttt tttcagcagc tttaagatct gcgactgaat cgtcacatca agagctgttg
      721 tcggctcgtc tgcaatcaga agcttcggtt ctgtaataag cgccgcagcg atcatcaccc
      781 tctggcgctg accgccggag agctggtgcg ggtatttttt gcaaagcttt tccgggtcgg
      841 gaagctcgac ctcatgcatt atggcaagaa ccttttcatg acgttctgcc ggtgtgcagc
      901 tctcatgaat cagcagtccc tctgcaatct gttcttcgat ggtcagaacc ggatttaaag
      961 aggtcatcgg ttcctgaaaa atcatgctga tttcttttcc gcggatgcgc tgcagatcct
     1021 ggctggaagc gtgcgcaacg gaagaatctt cgtagagaat ttctccgccg gatacggcgg
     1081 aattttcctt taaaaggtgc agaatggaga gcgccgtctg ggttttgccg gaaccggatt
     1141 ccccgacaat gccaagcgtc tcaccttctt ctaaggaaaa ggagatgcct tttaccactt
     1201 cggtcggtgg cgtggtatcg taaaatgcaa tttttaagtc acgtacatcg agaagtgcca
     1261 tatcagttac cgcctttctt ctgtaatccg tcgccgagga gcgatacgcc aagcgccagt
     1321 aaaatgacgg caagaccggg gaaaacagag ccccagggag cagtcatcag atacgtctgg
     1381 gattctgaga gcatccgtcc aagcgaggca tcgggcggct ggacaccgat gccgagaaag
     1441 ctcatgccgg cctcggagag aacggcgttg ttgaaaccga ttgctgccat cgaaagaagg
     1501 gacgggaccg tatttggcag gatatgtaca aatatgatcc gaagatggga aacacccatc
     1561 agacgggcac ttttgacata gtccatatct ttgcgtgcga gaaattcgct tctggtgata
     1621 cgtgcaaagc tcggaatgaa tacgataccg agtgcaagga tcacgttgta tttgccggga
     1681 cccagcagcg cgataaatac cagcgccagc aggattcccg gaaaggaaac aatgacatcg
     1741 tttatgcgca tcaggatttc gtcaatccat ccgccaaaat acccggtgaa cgcaccgaca
     1801 agaacaccaa tggttccgcc gagcagcaga gtagcgaaag cgacgaagaa ggttgtaccg
     1861 gttcctttta agacgcggct gaaaatatcg cgtccaaagt gatcgcagcc gaacgggtgc
     1921 gacagagatg gagcagcaaa cttggctgcg ccgttcattg catccgggtc gtacggtgtg
     1981 tagaaaaagc cgatcagaag aaacagaagc ataaaagcgg tgagaacagc tccgatgatc
     2041 agcgtgagat tgcgtttctt tttcataatg aaaacccttt cgtttgtcgt gggcggctcc
     2101 ctgcgaccga aaggtcgcag ggagcggatg atcgaaagct cccgtcagtc ttttacgcgg
     2161 atgcgcggat caattctgcc atacagcaga tctaccacga aattgatgat aataatgagg
     2221 gcggcgatca gtacaaggat gccctgaacc acggggtaat cgcggttgga gatcgaagtg
     2281 atcagaagat tgcccatgcc cggaatcgaa aaaacctgtt ccagaataat gctgtttgcc
     2341 acaatatctg cgattgtcat gccccagaag gtcagtaccg gaatcagggc gtttttcagc
     2401 acatggtgat acataacacg gcgtgtactg tttccgcggg aataggcggt gcgcacatag
     2461 tcggcatcga gctgcgagag cacagagctg cgcagcattt tgacagccat ggaacagcgc
     2521 ggaagtgcaa tggcggcaga cggtgcaaca agataaccga taaaaccgga aaaattttca
     2581 gaaatcggca cataggcgcc cggctgaaac catttgagaa tcagaccgaa aagatacgtg
     2641 atcagaatac cgatgaaaaa tcccggcaca gacatgatca cctgattgat cgtcatgatc
     2701 agccggtcca gatggcctcc ggcgtgctgt gctgtgaaaa tgccgatggg aatcgagaca
     2761 acaaggatca tcaggaatgc gataacggtg atagcgatgg taatcggaac tttatcgcgg
     2821 atcagttctc cgaccggcat cgagtacgaa tacgaggtgc cgagatctcc atgaaaaaag
     2881 ccggttaccc acgaagcata ccgttccaga accggccgat ccagtcccat ttccttttgc
     2941 agagcctcaa tccgctccgg cgtcgcctcc gttccaagct gcgattttgc cgcatctccg
     3001 ggtatgacgg aaaaggcaaa gaacgataaa aaggatacaa gaagcagcgt aagaaggaga
     3061 gagccgaatt tctttgcaat gtatttcatg aatggaaatt cctccttgtg taatgtagga
     3121 tactccggga atttttcccg gcttataaaa gcaggcacag aaaagcttcg ggcgtatcgg
     3181 ctgaaggttt tcgtgcacgt catttttatt gtagacaatg caggtgtaaa tagcaagata
     3241 aaattggaat atctcgaatt acagaataac aggaaaaggc ctcttgcata accggtgcgg
     3301 atgatgcaaa aagcctttga taaattgcct tcgataaatc ccctgtttag ttttccattt
     3361 cccgtccgag atacgttgcc gctacctgga ggagcttctt ttccgtgtcg cccggcaggg
     3421 actccctgtc tttgccgcag agaatgaccg aaccgatggc atcaccggag gagagaatcg
     3481 gaaaaatgag ctccggcaga ggagtagtat tgtctgtgtt ttccaaaatt gaaaaataag
     3541 ccttttcacc gggacgcgca agtactgttt tccggttcag aatcgcctcc tccagccggc
     3601 tgctgatgga cttgccaaga taatttttct gttccggccc gtttgccgcc acaatctgtt
     3661 cctgatccga aacacagacg agacatccgg tcaccgttga aatcgatttt gtaaaccctg
     3721 ctgcgcattc cgccagctct tcgatcggtg agtatttctt caggataatc tcaccctccc
     3781 gtccggtata aatttccatg gcctgtccct cccggatccg catcgtgcgc cggatttcct
     3841 tgggaactac cacacgcccc agatcatcaa tccttcgaac aattcccgtc gctttcatat
     3901 gaaaaattcc tcctgtttcc gcaatgtact ttccataatt tgtgctgtat gggtagtatg
     3961 tggaaaaaca ggtggaatta tgcatggaaa gaagaaacgt atttccgctg tttacagcaa
     4021 tttcggaaaa aatacagtgg aacagcagtg gttagtctgg ttgctccttt tacagaagac
     4081 tgcggtcttt tccgagatag aacaaagcga tcgtatccgg tccgacatgg cttccggtgc
     4141 agtaatcgta agaagtattg ataaattcac gcaccttcac tcttttctgg atttcttcca
     4201 tcatataaag agattcctca taggcatctg catgtgcgat tccgacgatc tgcttttccg
     4261 gttcttcgat cgtttcacag aagagacgga tcagttcatt taaaacggcc ttgcgcccgc
     4321 ggacattctt gtaggaaaca atatagccat cttcacttcc acgcagaatc ggtttgattt
     4381 tcagcatatt tccgaccaca gcgacacttc ccttgattct tccggtacgg gaaagatatt
     4441 tcagatcgcc gacggtgaaa acaccgttca ttttgctaac gtaggtttcc agaagatctg
     4501 ccgcttcgtc tacgttcatg ccccgatcgc gcatttcgct ggcatagatg gcgaggatgc
     4561 cctgtgcaag agaggcattc agggaatcga cgaggcggat tttacgtttt ccgccgtatt
     4621 cctcaagaag ctcctcggca gcaagtcttg cggagttata agttccgctg atgtttttgc
     4681 ttaaggaaag atagatgaca tcctgatctg cctcaagagc ggtgcggaac gcatccgcaa
     4741 aatttgcggt gctgatcagt ccggtcttga tttcacagcc ggtgcgcatg gcattgtaat
     4801 attcttttcc ttttgcaagc tcctcttccg gtgacagatc cgggtcgaag cagaccagct
     4861 cttttccatt cacaagattg acgaaagaaa cgacatggat atcgtatttt ttcgcagtaa
     4921 cagcaggcag atttgccgct gaatcaacaa aaacctgaaa cataaataaa attctccttt
     4981 gtagtgtaaa tgtcccccaa aatgatgtaa cttttgataa gcattgtagc acattgtatc
     5041 accaaagaag agagatttca atggaagata gacaacatac tacaaaggag tgtgccgaca
     5101 agttgattcc tgaaatcatc tgttttcgga agaaatttga ccaggtatca atgcatagta
     5161 ataatgacca aaaacggcag acgtattttt agtaaaaacg gcttttgaaa caaaaataaa
     5221 atcgttttat aatagaatca tagcgagtag tgtaatgatg tggaaaaaac catatgatac
     5281 tgctcgcttt tataaaacta ttttcaggag gaaccgtata tgcatgagta tgaaatcttt
     5341 attgaagaaa taaatccgtg tggaggggag aaacatagta aaaaaacttt gatcgaagca
     5401 gaagcagtca gtccggaagc ttatgtaaaa gaaaacggaa gatttccggt tcttgaatcc
     5461 atgtgcaatg aaagtggaga tgttgtaata gtaacaggag ataataaag
//
LOCUS       DAOMUF010000144         5493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4086_length_5493_cov_4.401067, whole genome shotgun sequence.
ACCESSION   DAOMUF010000144 DAOMUF010000000
VERSION     DAOMUF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_5493_cov_4.401067"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLN77_08225"
     CDS             complement(<1..609)
                     /locus_tag="PLN77_08225"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRM44294.1"
                     /translation="MGYKSDIEIAQECTMQPITAIAEKAGIDDKYLEQYGKYKAKIDY
                     NLLKDTNAEDGKLILVTAINPTPAGEGKTTTSVGLVDGLSRIGKKAMVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMIDNHIFQGNALNIDPR
                     KITWKRCVDMNDRQLRSVVDGLGGKTNGMPREDGYDITVASEI"
     gene            complement(838..2604)
                     /locus_tag="PLN77_08230"
     CDS             complement(838..2604)
                     /locus_tag="PLN77_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRM44295.1"
                     /translation="MKHKKQKKTKKAGRILLAVFAVLIGTLGVLLFTSERWMRKTWPN
                     LSMEELVYQLKAPIAGTSHDLLMSYICSSALIAAAALILLILVSIFLREKRKAHVAFG
                     MICIAAGAASIVYSINDVWAALDVKDYLKNQSTYNTVVEDNYVDPDRVNITFPEQKRN
                     LIYLYLESMESTYADKASGGAFDKNYIPELTQLAADNISFSNSELLGGGQPTVNATWT
                     IAGIFAQTSGLPLSIGIQRNEMAYQASFFPEINTLGDVLADEGYKQYFFIGSIGQFGG
                     REEYFKEHGDYEVDDYNWAMEKGLIPEGYYEWWGYEDEKLFSFAKDRLTEIAAEGEPF
                     NFTMLTADTHFEDGYPCELCDEENDGDNQYGMVLHCSSKQVTEFVSWIQQQDFYENTT
                     IVISGDHLTMDSDFCENIDPDYTRTVYNVIINSPIQPQQEKNRSFTTMDMFPTTIASL
                     GATIEGDRLGLGTNLFSGEQTLAEKLTFDQLNDDLSQKSKFFEKMEEQVTSIWTKTDE
                     GWKFYIEDEDRWAKSEWVSLNPHRYANDTEQRYYIDANGYAVKGWKLIDGKWYYFSTQ
                     GSYRLLEGPCDEPFEVDESQYS"
     gene            complement(2836..3459)
                     /locus_tag="PLN77_08235"
     CDS             complement(2836..3459)
                     /locus_tag="PLN77_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRM44296.1"
                     /translation="MDPNTFPTKGNLILAKNSLKLAHMGYELMDKKRNILIRELMGLI
                     DEAKDIQRQIDVTFREAYAALQIANMEIGITNVQTVSYTVPVEESIRIKTRSIMGVEI
                     PLVEADPSGQAPTYAYYSTKESLDQARQAFEKVKDLTLNLSMIENSAYRLAAAIKKTQ
                     KRANALKNITIPHYTQLSKDIADALEEKEREEFTRLKVIKQRKNKAS"
     gene            complement(3480..4904)
                     /locus_tag="PLN77_08240"
     CDS             complement(3480..4904)
                     /locus_tag="PLN77_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRM44297.1"
                     /translation="MAIEYLGLSEVNGPLVVLEGVKNASYEEIVEFTVDGNEKKLGRI
                     VAVYEDKAVIQVFEGTSSMSLTNTHTRLTGHPMEIGLSEEILGRTFDGIGKPIDRMGP
                     IDAEVRRNVNGLPLNPVTRKYPRNYIHTGISAIDGLTTLIRGQKLPIFSGNGLPHDQL
                     AAQIVQQASLGDSDEKFAIVFAAMGVKYDVAEFFRRTFEESGVSDHVVMFLNLANDPV
                     VERLITPKVALTAAEYLAFEKGMHILVILTDITSFCEAMREVSSSRGEIPSRKGYPGY
                     LYSELATLYERAGIVQGVEGSVTQIPILTMPNDDITHPIPDLTGYITEGQIVLDRQLH
                     GQSIYPPISVLPSLSRLMKDGIGEGYTRADHQDVANQLFSCYAKVGDARSLASVIGED
                     ELSPIDKQYLVFGNEFEHEFIGQGMDENRSMEDTLNKAWELLGLLPREELDRVNTKVL
                     DQYYHPTTIDAVAKAKAEADAMNE"
     gene            complement(4919..>5493)
                     /locus_tag="PLN77_08245"
     CDS             complement(4919..>5493)
                     /locus_tag="PLN77_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRM44298.1"
                     /translation="EPVTMNTKRFVRCFWGLDKSLAYARHFPAIHWLTSYSEYLQDLS
                     PWYKDHVNKNFVDMRNQLMGILNSESSLMEIVKLIGSDVLPDDQKLTLEIARVIRLGF
                     LQQNAFHPDDTCVSLEKQYKMMEVILYLYKKSKALVTMGMPMSVLKEDKIFDRVVSIK
                     YDVPNDRLDLFDTYKKDIDTFYDKVLEKNG"
BASE COUNT         1229 a         1386 c         1301 g         1577 t
ORIGIN      
        1 gatctcagat gctacggtga tatcgtagcc gtcttctctc ggcattccgt ttgtttttcc
       61 gccaaggccg tctacaacgc ttcgaagctg acggtcattc atatctacgc atcgtttcca
      121 ggtgatcttt ctcggatcga tattcagagc gttgccctgg aaaatgtggt tgtcgatcat
      181 agctgccagc aggttgtttg ctgcaccgat tgcatggaag tcaccggtga agtgaaggtt
      241 gatatcttcc attggaacaa cctgtgcgta tccgccgcct gctgctccgc cttttacacc
      301 gaagaccggt ccaagagatg gctcgcgaag tgcaaccatt gcttttttac cgattctgga
      361 aagtccgtct accagaccta cagaagttgt tgtttttcct tctcctgccg gtgtcgggtt
      421 gatggctgta acgaggatca gttttccatc ctccgcattg gtgtctttta acaggttgta
      481 atcaattttt gctttgtact ttccgtactg ctccagatat ttgtcatcaa tacctgcttt
      541 ttctgcgatc gctgtgatcg gctgcatggt acattcctgt gcaatttcaa tgtctgattt
      601 gtaacccatt gttttaaaat cccctttctt ttttccgctt tgtccgccga ttgatagcca
      661 tattttacca tgttttgtaa gatgattcaa ttgataatta tttatttcac aatctttata
      721 cgttttgtac caaaaaatgc agaaaattcc gccaaaaggc ggagcggcac aattctgcat
      781 taaaaaaaga aggattcccg gctttttgct gccgaaaatc cttctctttt ttatttttta
      841 agaatactgc gactcatcca cctcaaacgg ttcgtcgcac ggtccttcga ggaggcggta
      901 gctgccctgt gtggagaagt aataccattt tccgtcgatc agtttccatc cttttacggc
      961 gtagccgttt gcgtcaatgt aatatctctg ctcggtgtcg tttgcatagc ggtgcgggtt
     1021 cagggaaacc cattcgcttt tggcccagcg gtcttcgtct tcgatgtaga acttccagcc
     1081 ctcatcggtt ttggtccaga tggaggttac ctgctcttcc attttttcga agaatttgga
     1141 tttctgggag agatcatcat tgagctggtc gaaagtcagc ttctctgcaa gcgtctgttc
     1201 acctgaaaac aggttcgttc caagaccgag gcggtcaccc tcaatggtag ctccgagact
     1261 tgcgatggtg gtcgggaaca tgtccatggt ggtgaaactt ctgtttttct cctgctgcgg
     1321 ctggatcgga gagttgatga tcacgttgta tacggttctg gtgtagtccg gatcaatgtt
     1381 ctcacagaaa tcggaatcca tcgtcagatg gtcgccagaa attacgatcg ttgtgttctc
     1441 gtagaaatcc tgctgctgaa tccaggaaac gaactcggtg acctgcttgc tggagcagtg
     1501 cagaaccatt ccgtactggt tgtcaccgtc gttttcttca tcgcagagct cacacggata
     1561 tccatcctca aaatgggtgt ctgcggtcag catggtgaag ttgaacggct ctccctctgc
     1621 tgcgatttcg gtcagacggt ctttggcaaa ggaaaagagt ttttcatcct catagcccca
     1681 ccattcgtaa tatccttccg ggatcaggcc tttttccata gcccagttgt aatcgtctac
     1741 ttcgtagtcg ccgtgctctt taaagtactc ttcccggccg ccgaactgtc cgattgaacc
     1801 gatgaagaaa tactgcttgt agccttcatc cgccaggaca tcacccaggg tattgatttc
     1861 cgggaagaag ctggcctgat atgccatctc atttcgctgg attccgatgc tgagaggcag
     1921 accggaggtc tgtgcgaaaa ttccggcaat ggtccaggtt gcgtttacgg taggctggcc
     1981 gccgccgagc agctccgaat tggaaaaact gatgttgtct gcggcaagct gggtcagctc
     2041 cgggatatag tttttatcga atgctccgcc ggaagctttg tctgcgtagg tgctctccat
     2101 ggattccaga tagagataga tcagatttcg tttctgttcc gggaacgtga tattcacgcg
     2161 gtccggatct acgtagttgt cttctaccac ggtattgtac gtgctctgat ttttcagata
     2221 atcttttacg tccagtgctg cccacacgtc attgatggaa tagacgatgc tggcggctcc
     2281 tgccgcgata cagatcatcc cgaacgccac atgggctttg cgtttttccc gaagaaaaat
     2341 gctgaccaga atcagaagga tgagtgctgc cgctgcgatc agcgcacttg agcagatata
     2401 actcatcaga agatcatggc tggttccggc aatcggggcc tttaactggt aaacaagctc
     2461 ttccatgctc agattcggcc atgttttccg catccagcgc tcggacgtaa acagcagcac
     2521 gccaagcgtt ccgatcaaca ctgcaaacac agccagcaga atgcgccctg cttttttcgt
     2581 ttttttctgt ttcttgtgtt tcatcttcct acctccaaaa agcattacag cttacaaaat
     2641 cttaactttt ctttacaatc gccttacgta ttatagcaaa gaccgtttta aaaagataga
     2701 aaatttttat aaaatttttg cacaaaaagt gcagccaaaa acatattttt tgcatgacac
     2761 ataaaaaccc gcccggcacc gcaaatctct tcgcggccca agcgggtttt tcatctctat
     2821 tccgggaaag ccttatcagc ttgccttgtt ctttctctgt ttgatgacct tcagtcgtgt
     2881 gaactcctcg cgctccttct cctccagtgc gtccgcgata tccttggaca gctgggtgta
     2941 atgcggaatc gtgatgttct tcagcgcgtt ggcacgcttc tgtgttttct tgattgccgc
     3001 agcaaggcgg tacgccgagt tctcaatcat ggaaaggttt aaggtcaggt cttttacttt
     3061 ttcaaaggcc tgtcttgcct gatccaggga ctccttcgtg ctgtagtaag catacgtcgg
     3121 ggcctgtccg gacggatcag cctctaccag cggaatttcc acgcccatga tactccttgt
     3181 cttgatccgg atagattcct ccaccggaac ggtataggac acggtctgta cgttggtgat
     3241 accgatctcc atattcgcaa tctgcagtgc cgcataagcc tcgcggaatg tcacatcaat
     3301 ctgacgctga atatcctttg cctcatcgat caggcccatg agctcgcgga tcagaatatt
     3361 ccgcttcttg tccatcagct cgtaacccat gtgggcaagt ttcagagagt tctttgcaag
     3421 aatcaggttt cccttggtag gaaaggtatt cgggtccatt ccgatctcac tcctctcgct
     3481 tactcattca tggcatcggc ttccgccttt gcttttgcta ctgcatcaat ggtggtcgga
     3541 tgataatact gatccagaac cttggtgttg acacggtcga gctcttctct cggaagcagt
     3601 ccaagaagct cccatgcctt gttcagcgta tcttccatgg aacggttttc atccattccc
     3661 tggccgatga attcatgctc gaattcatta ccgaatacca ggtactgttt atcgatcgga
     3721 gaaagctcat cctcaccgat aacggatgcc agggaacgcg catctcctac ctttgcatag
     3781 caggagaaca gctggttcgc tacgtcctga tgatcggctc ttgtgtagcc ctcaccgata
     3841 ccatccttca tcagacggga aagactcggc agtacgctga ttggcgggta aatagactgg
     3901 ccgtgaagct gacggtccag tacgatctga ccctcggtga tgtaaccggt caggtcaggg
     3961 atcgggtgcg tgatatcatc gtttggcatg gtcaggatcg gaatctgtgt tacggatccc
     4021 tctacgccct gtacgattcc ggcgcgctca tacagcgtag ccagctcact gtacaggtat
     4081 ccaggataac ctttacggga aggaatctct ccacgggagg aggaaacctc acgcatagcc
     4141 tcgcagaagg atgtgatatc ggtcaggata accaggatat gcatgccttt ctcgaatgca
     4201 aggtactcgg cagctgtcag cgctaccttc ggagtgatca gacgctctac aaccgggtcg
     4261 tttgccaggt tcaggaacat aacaacgtgg tcagatacac cgctttcctc aaaggtccgg
     4321 cggaagaact ctgctacgtc gtatttaaca cccatagcgg caaatacgat tgcaaacttc
     4381 tcatcagaat ctccaaggga tgcctgctgt acgatctgtg ctgccagctg gtcgtgcgga
     4441 agtccgtttc cggagaagat cggcagcttc tgtccacgaa tcagggtggt cagaccatcg
     4501 attgcagaga taccggtatg gatatagttt cttgggtatt tacgggtaac cgggttcagc
     4561 ggcaggccgt tgacgtttct tcttacctct gcgtcaatcg gtcccatgcg gtcgatcggt
     4621 tttccgatac cgtcgaaggt acgtccaagg atttcttcgg aaagtccgat ctccatcgga
     4681 tgtccggtca gacgggtatg ggtattggta agagacatgc tcgaagttcc ctcaaatacc
     4741 tgaataacgg ctttgtcttc gtatacagca acgatacgtc ccagtttttt ctcattgccg
     4801 tctacggtaa attctacgat ttcctcgtag gaagcatttt ttacaccttc cagaacaacc
     4861 agagggccat tgacctcact aaggcctaaa tattcaattg ccatgatgaa ttccctcctt
     4921 aaccattctt ctcgagtacc ttatcgtaga aggtgtcgat atcttttttg tatgtgtcaa
     4981 acagatccag acggtcattc ggtacatcgt atttaataga aacgacgcgg tcgaagatct
     5041 tgtcctcttt cagtacggac attggcatgc ccatcgtcac gagcgctttg gatttcttgt
     5101 acagataaag aataacttcc atcatcttgt actgtttttc cagagatacg caggtgtcat
     5161 ccggatggaa tgcgttctgc tgcaggaagc ccagtcgaat aactctggcg atttccaggg
     5221 tcagcttctg gtcatccgga agaacatcgc taccgatcag ttttacaatt tccatcaggg
     5281 agctctcgga gttcaggatt cccattaact ggttccgcat atctacaaag tttttgttta
     5341 cgtgatcttt gtaccacggc gacagatcct gcaggtattc actgtaactg gtcagccagt
     5401 ggatcgccgg gaaatgacgg gcgtaagcca gagatttatc cagtccccag aagcagcgga
     5461 cgaaacgttt ggtgttcatg gtaacaggct cgg
//
LOCUS       DAOMUF010000145         5378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4196_length_5378_cov_3.788277, whole genome shotgun sequence.
ACCESSION   DAOMUF010000145 DAOMUF010000000
VERSION     DAOMUF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4196_length_5378_cov_3.788277"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1507)
                     /locus_tag="PLN77_08250"
     CDS             complement(290..1507)
                     /locus_tag="PLN77_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM44299.1"
                     /translation="MVERKEYLDQLWAWKDERQIKVVTGIRRCGKSVLLEQYQQRLLA
                     NGVAPEQIISINFENLDYEPLKDYMKLYNYLKERLCADKMTYIFLDEVQEVPSFEKVV
                     DSLYIRDYVDVYITGSNAYMLSSELATLLSGRYTEIKMLPLSFREYMAVTGMAKEEAF
                     AEFMKTGGIPYVAVMNRTDEKIDQYLEGIYNTVIVKDIEQRQTRREKESGKRKITDIA
                     LLKTIARYLASVIGSPVSMKSITDYLTSAGRKISQNTVSDYVEALTESFVFYPVERFD
                     IVGKQLLKVNNKFYMVDMGIRNHILPRNRYDLGFTIENIVYLELSRRGGKVNIGKYGS
                     TEVDFVTQKEGVLTYYQVTADMTAEETFEREMRPLRSIQDNYEKIVLTLDRFSLGNYD
                     GIKVMNVIDWLLG"
     gene            complement(1786..2178)
                     /locus_tag="PLN77_08255"
     CDS             complement(1786..2178)
                     /locus_tag="PLN77_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM44300.1"
                     /translation="MKLAAIHHVAIIVSDYEKSKDFYVNKLGFAIVRENFRKERNDWK
                     LDLRVGEGADAIELEIFAEPNPPKRVNRPEACGLRHLAFRVEDVEATVAELEQMGIGC
                     EPIRLDSYTQKKMTFFFDPDGLPLELHE"
     gene            complement(2197..2508)
                     /locus_tag="PLN77_08260"
     CDS             complement(2197..2508)
                     /locus_tag="PLN77_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44301.1"
                     /translation="MSITRKLAKLILTLLTLPLLVVVWLLKWFVMFLYCCSAWIFYLL
                     GSVLFATAVLSFLMKQSQGMEALQMLIGGFVIFMIPQVVGSVAVFLELVAATIRQVWY
                     I"
     gene            complement(2595..4211)
                     /locus_tag="PLN77_08265"
     CDS             complement(2595..4211)
                     /locus_tag="PLN77_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM44302.1"
                     /translation="MFYYKEKKNKGIRAAAYLRLSIEDGDKAESNSIGNQRELIQNFV
                     AERPELHLVGEYADDGYTGTNFDRPGFQKMMDGVRSGEIDTIIVKDLSRFGRDYIGVG
                     EYMEQIFPLLGVRLISVNDNYDSNNYNGATLGMDLVVSNLVNTMYCRDAGKKLRTANR
                     VKWRKGISTASSAPFGYQFDPNKKGSYIIDPPAAKIVRRIFDLAILGLGTREIAMTLN
                     DENAPVPSVYNREHKAYGKETTYTIAPVILWDSTRVWKILTAYVYTGAMVLGKSQRLI
                     SGKSISRTVPKGQQYITEGTHEAIVSREEFEKAQLVINNRNKVVMGSVDFPLKGKVRC
                     GNCRRAMGYDYKQASPIFWCREGQELIGQTKCSSEIFQANDIENAVFQALKKELSLLG
                     SLYGDIKKEEQSLKEASRKASRRKTSMEQELKNLKGEKMRMYEEYAAGTLSLDNYKNK
                     KQEYDRKIAEVQDKIEQSKAEEAAQSVVPGTVRAAAEQAENFLHGTRLTASMVSAFIE
                     NVYVHEGGRIVVQFKYEQSIQDAVRALHTD"
     gene            complement(4366..4527)
                     /locus_tag="PLN77_08270"
     CDS             complement(4366..4527)
                     /locus_tag="PLN77_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44303.1"
                     /translation="MKQKERMDKKVKQERTEPDHHRKIIIKGITVYESFRPEGSELWD
                     CLLQSMGKH"
     gene            4918..5310
                     /gene="mobC"
                     /locus_tag="PLN77_08275"
     CDS             4918..5310
                     /gene="mobC"
                     /locus_tag="PLN77_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HRM44304.1"
                     /translation="MARPKKEESIRRTSNVMVRFSSIEYELVAGYAKDAGYPISTFVR
                     KQALSEKVTVNYNIVADIGEIQKLTAQAAGIGNNLNQIARYFHGGGLASRGMLEELKK
                     CIAEIRELRQEIVQLGGVYRGNSKTHRK"
BASE COUNT         1366 a         1386 c         1002 g         1624 t
ORIGIN      
        1 gtcattccag catctttcgc tgcttcttca atcgtcacgc ggccctttct caccaactta
       61 accaacatcc gcatctcacc aatgttaatg ccttcttctc tgccttcggc cttcacgcct
      121 tgactaaggt tgcacacaac aaacacctct ctttccagtt cttcgttcat tgcaacgcca
      181 aattcttctt caagattttt ctctgtggtt gctctggtcg aatggaataa cgaaccagag
      241 tgttttggaa ctccttggtt gggtgtgtac aaacccagat gcagattcct catcccaaaa
      301 gccagtcgat gacattcata acttttatgc cgtcgtaatt tccgagcgaa aaacgatcca
      361 gtgtcaggac aatcttttca tagttatcct gaatgctccg cagcggtctc atctctctct
      421 caaaggtttc ttccgccgtc atatcagcgg tcacctgata ataggtaagc acgccttcct
      481 tctgtgtcac aaagtcaact tcagtagaac catattttcc gatattgact ttgccgcccc
      541 ttcgtgaaag ttcaagatac acaatgttct caatggtaaa tccaagatcg tatcggtttc
      601 ttggaagaat gtggtttctg attcccatat ctaccatgta aaacttattg ttgaccttta
      661 agagctgctt tcctacaatg tcaaatcgct ccacaggata aaaaacaaaa gactctgtca
      721 gggcttcaac ataatcgctg actgtgtttt gggatatttt tctgccagct gaggtcagat
      781 agtcggttat acttttcata gacaccgggc tgccgataac gcttgccaga tatctggcaa
      841 ttgtcttcag taacgcaatg tctgtgattt ttcgttttcc actctctttt tctcttcttg
      901 tctgtcgctg ctcaatatcc tttacaatga cggtgttgta gattccttcc aaatactgat
      961 ctattttttc atccgtacgg ttcattactg ccacatacgg aattccgcca gttttcataa
     1021 actctgcaaa agcttcctct tttgccatac ctgtcaccgc catatactcg cggaaagaaa
     1081 gcggcagcat cttgatctct gtgtaccttc ctgacaaaag cgttgcaagc tcacttgaaa
     1141 gcatatatgc attggagccc gtgatataaa catccacata atccctgata taaaggctgt
     1201 caaccacttt ctcgaatgat ggaacttcct gaacctcatc caaaaagatg tatgtcatct
     1261 tatcagcaca caaacgttct ttgaggtagt tgtacagctt catataatct ttcaagggtt
     1321 cgtaatccag attttcaaag ttgatagaaa taatctgctc tggtgcgact ccattcgcta
     1381 agagtctctg ctgatactgt tccagaagta cagattttcc acagcgacgg attccggtca
     1441 cgaccttgat ctgccgctca tctttccatg cccaaagctg gtcaagatat tcttttcgtt
     1501 caaccatatc tgcattcctc ctttttctca gtatagcata ttatacgcag aatatcaaat
     1561 tgttttcccg tttcggaaac ttttatttta tcatactcaa tattttctaa ttatggaaat
     1621 tgtctgatac gaggcatgaa aaagtgttta actccaactc caccaaagca gatctattcg
     1681 catgggtaga aacgctcaat ctataacctt cccattccta tggaatgaaa caccctcata
     1741 gcagagaaga tttacgaatc ctctcaacta tgagggcgtt gttttttatt catgcagttc
     1801 caatggcaaa ccatctggat caaagaaaaa tgtcatcttc ttttgggtgt agctgtccaa
     1861 tcgaatcggt tcgcatccta tccccatctg ttccagttct gctacagtgg cttccacatc
     1921 ctccacccgg aatgctagat gccgcagtcc gcaggcttcc ggtcgattca cgcgcttagg
     1981 cgggttcggc tctgcgaaga tctccagttc aatcgcatca gcaccctcac caacacgcaa
     2041 atccagtttc cagtcattcc gttctttacg gaagttttct cgtacaatcg caaatcccag
     2101 cttattcaca tagaagtctt tcgatttctc ataatctgaa acgatgattg ccacatgatg
     2161 aatcgctgct aatttcatat tctaggtttc acctccttat atgtaccata cttgccgaat
     2221 cgttgctgcc accagttcca aaaacacagc cacgctacca accacctgcg gaatcataaa
     2281 aatcacaaat cctccgatca gcatttgaag tgcttccatt ccctgcgact gcttcatcag
     2341 gaatgaaagc actgccgttg cgaacagcac actgcccagc agatagaata tccatgctga
     2401 acaacaataa aggaacatca cgaaccattt cagcagccat actactacga gtagcggcag
     2461 ggtcaacaag gtcaatatca gttttgccag ctttcgggtt atactcattt cgtcctcctt
     2521 ccggttggtc tgttgcccat attatatcac actcaccaat gtaatagcat gggcaacttc
     2581 gccttttttg atatttaatc tgtgtgcaat gctcttacgg catcctgtat actctgctca
     2641 tatttgaact gtacaacgat tcgcccaccc tcatgcacat aaacattttc gatgaaggcc
     2701 gataccatgc ttgccgtaag tctcgtgccg tgcaggaaat tttctgcctg ttctgccgct
     2761 gcgcgtaccg ttcctggaac aacgctctgg gcagcttctt ccgccttgga ttgttcaatt
     2821 ttatcctgca cctctgcaat cttcctatca tattcttgtt ttttgttctt atagttgtcc
     2881 agagagagcg ttccggctgc gtattcctca tacatccgca tcttctcgcc tttcagattt
     2941 ttcagttcct gctccatcga tgtttttcga cggcttgcct ttctactggc ttctttcaag
     3001 ctctgttctt cctttttaat atcaccatag agagagccta ataaagaaag ttcttttttc
     3061 aatgcctgaa atactgcatt ttcaatatca ttggcctgaa atatctccga tgaacatttg
     3121 gtctgcccga taagttcctg tccctctctg caccagaata tgggagaagc ctgtttgtag
     3181 tcatatccca tagcccgtct gcaattaccg catcgaactt ttcccttaag tggaaaatcc
     3241 acgctgccca ttactacttt attgcggttg tttatgacaa gctgcgcttt ttcaaattct
     3301 tcccggctga cgatggcttc atgggttcct tccgtaatgt actgctgccc tttcggaaca
     3361 gtacgagaga tagacttacc ggaaatcaac ctttggcttt tccccagaac catcgcaccg
     3421 gtatacacat acgctgtgag aattttccag acacgagtgc tatcccaaag tatcacaggc
     3481 gcaatcgtgt aagttgtctc ttttccatac gccttatgct cccggttata aacactcggc
     3541 accggagcat tttcatcatt cagcgtcatc gctatttctc gcgtccccag tcccagaatc
     3601 gcaaggtcaa aaatacgacg gacaatttta gccgctggtg ggtcaatgat ataggatcct
     3661 tttttattcg ggtcaaactg ataaccaaac ggtgcagaag atgctgtaga aatcccctta
     3721 cgccacttca ctcgattggc tgtccgaagt ttctttcctg catcacggca gtacatggtg
     3781 tttaccagat tgcttacaac caaatccatc cccagtgtcg caccattata attgttgctg
     3841 tcatagttgt cattgacgga gatgagccgg acacccagca gtggaaaaat ctgctccatg
     3901 tattcgccca caccaatgta atcacgtcca aaacgagaaa ggtcctttac gataatggta
     3961 tcaatctcgc cactgcgcac accatccatc atcttctgaa agcccggtct gtcgaaattg
     4021 gttcccgtgt aaccatcatc tgcatattct cctaccaaat gcagttctgg tcgttctgca
     4081 acgaaattct gaatcaattc tctctggttt ccgatgctgt tgctttcagc cttatctccg
     4141 tcctcgatag acaatctcag ataggccgct gctcgtatgc ccttattctt tttttcctta
     4201 taataaaaca aagccattaa acctcccgtc tccacgcaat tcgcatggta aaatccagaa
     4261 agttaatggc ttttgattgc acatatttac ttaacccgtc tgtattctac caagctgtag
     4321 cacaaaaatc aagctgtagc gtacattttt cgaaaaatct ttttttcagt gtttccccat
     4381 gctctgcaag aggcagtccc acagttcact gccctctggc cggaacgatt catacacggt
     4441 gatgccctta atgatgattt ttctgtgatg gtcaggctcc gttcgctcct gcttcacttt
     4501 tttgtccatt cgctctttct gcttcatcgg atcaatcctc ctttactaat aggaatccag
     4561 aagggcattt tggaatacag tttttgaaag aaaatttaaa aaagctgaaa agtgtattcc
     4621 aaataaaggc tgcggattcc tatttataga agcatctttt tagggtaagc cttcggatta
     4681 cggcagaagt ctggggcttt tctctcttag cgcaagattc gcccatagta actcaaaact
     4741 cactttcgtg atttttcgtt cctggcaatc ttgatgggga ttccgttccc cattcccacg
     4801 gtacgcacaa aatcttggat tttggctatc tggctccaca cgaatgtgtt cgcagccgaa
     4861 cgcctacggc ttatcggaag ttgcttcgca actataaaaa cgcaggagga tcacactatg
     4921 gcacgaccca aaaaggaaga aagtatcaga cgcaccagca atgtaatggt tcgtttcagc
     4981 agcattgaat atgaactggt ggccggatat gcaaaagatg ctggttatcc catctctact
     5041 tttgtgcgca agcaggctct atctgaaaaa gtgactgtca actacaacat cgttgccgat
     5101 attggcgaaa ttcagaagtt gactgctcag gccgcaggca tcggaaataa tcttaaccag
     5161 attgcccgct atttccacgg cggcggtctg gcatcgcgcg gaatgttgga agaactgaaa
     5221 aagtgcattg cggaaatccg tgaacttcgg caggaaatcg ttcagcttgg aggtgtatac
     5281 cgtggcaatt ctaaaacaca tcgcaagtaa aaatgccaat tacgggagtg ccatcgacta
     5341 tctgaaatac cagcacgatg agtttcatct ggttcccg
//
LOCUS       DAOMUF010000146         5368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4206_length_5368_cov_4.441182, whole genome shotgun sequence.
ACCESSION   DAOMUF010000146 DAOMUF010000000
VERSION     DAOMUF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4206_length_5368_cov_4.441182"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1859
                     /locus_tag="PLN77_08280"
     CDS             159..1859
                     /locus_tag="PLN77_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRM44305.1"
                     /translation="MKEQQKKKAAPVLVVLILIVLVGAAGIVSFLINRYKPGTEYMAG
                     NEYFNLTDENSVALIQNGELLEEQAVLIGGEPYAAYTYVESQLNSCFYWDEETKGILL
                     TTSGGVQTLLPGDAAVAKTPGGQPAVQQESDGTVYISLDVVKEYTDLDYAYYSDPNRV
                     VIRNEWDGVEQATVQSDTAQVRQKGGIKSLILADVQKGDTLLYLENLDNWCKVMTADG
                     YTGYIQTEDISEPEAIEARTAKKDSYERITRDHKINLVWHQSTSTESNDAMAEMTAEM
                     TGVNVISPTWFSVTDETGTISSLASADYVKLAHEAGREVWGLIDNFNEAFDETTDLAY
                     ASVRSRIIEQLLAEAASCGMDGINVDFENLKEAGIPHYLQFLRELTSAAHAQNLVVSV
                     DTPVPQAYTMYYQRGEQARFVDYMIVMAYDEHFAGSEEAGSVSSLPFVQQAVEEMTRV
                     MPADQVICGIPFYTRVWTEKFGQSAITSEVLGMDGAKNYAKENQMTETWDASLGQNVA
                     TVETSDARYTIWMEDEQSMEEKLKVIQSADLAGVAEWKLGFECADVWSLISEYIETNS
                     "
     gene            1920..3002
                     /locus_tag="PLN77_08285"
     CDS             1920..3002
                     /locus_tag="PLN77_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44306.1"
                     /translation="MSENNHEELFKTTLMGGFDKDDVMTKVQNLKDQAYAAQKKLEAK
                     IEEKEQEIEKLNRKIREREDKIEELEKNIHEKYQSYIDNYDSISGLVFEAQVKAKKLT
                     QETEDACREKTEETDAACRKLTEETEESCRKLKEETEEACRRKTEETDESCREKTEET
                     DEACCKKREETNESCRKQREETKESCAKQRRETKEQCEYMVESARQKGEELAAVYEEK
                     LQAEAEEIQSHMDAELQKANTRYQKIYAEMETMTSVLEKNRRIFEETFAGIEKMREEM
                     KRSASEVETFMKDPVKSIFPGEAKETAAEPDREDTAEEAEEEDEIVENSAVIDLENEP
                     DYLDDDNDEEEDEIPNRAHDEEDEEE"
     gene            3115..3765
                     /locus_tag="PLN77_08290"
                     /pseudo
     CDS             3115..3765
                     /locus_tag="PLN77_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542289.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            3773..5047
                     /locus_tag="PLN77_08295"
     CDS             3773..5047
                     /locus_tag="PLN77_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRM44307.1"
                     /translation="MAKRRHRRRRKRPLRALLPLMLALLAAYLVFGEFDESDPAQNGS
                     GADSSTASQTADENNGLSENGATQFQAENTDEEELAKRYYYSQLDENRQMIYRELVQG
                     IAEHQETIITKGGDPDVTAEVYGWVYMDYPEYCWINGASHVTGYGEPKNYCEVVPEYT
                     IPAEEITGRQTQIKGAGNDFLSDIDRSMDDYGKIKAVFEKCIRQIDYVKDAPENQTLY
                     SGLVNGQTVCAGYARTFQYLMNRLDIPVIYVTGTTDTGEAHGWNIVKCGDNYYNVDVT
                     WGDPVFAEGESGEYNLPDDLIYYDFLCVSDAEFSDTHQADANLPLPACNADDLEYYRQ
                     IGRYMDTFDATQILWEMETDIEETKEYSEFKFATDDLMAQVMEARPELLDKASGYLCS
                     YYGLGSVKYTYSEDTATRKLMVFWQYKGEPSE"
     gene            complement(5103..5210)
                     /locus_tag="PLN77_08300"
                     /pseudo
     CDS             complement(5103..5210)
                     /locus_tag="PLN77_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
BASE COUNT         1580 a         1181 c         1594 g         1013 t
ORIGIN      
        1 gcgaatgtta cagttcgctg ggaaccagtg aactgtaacg atactttctg actcgcatgc
       61 ggttcccttg cgcataacgg ttgctattgg gtattttttt tgttataatg ggcgcaagtg
      121 gggagatctt cagagcgaag aaaaggggaa aagaacacat gaaggagcaa cagaagaaaa
      181 aagcagcgcc ggtactggtc gtactgattc tgatcgtgct agtcggtgca gcaggcattg
      241 tttcattttt gataaaccga tataaacccg gaaccgaata catggccggg aacgaatatt
      301 ttaacctgac agacgaaaac agcgttgcgc tgatccagaa cggagaactt ctggaagagc
      361 aggccgtcct tatcggagga gagccgtatg cggcgtacac atatgtagaa tcccagctga
      421 acagctgctt ctactgggat gaagaaacaa aaggaatcct gcttacaacg agcggcggcg
      481 tgcagacact gcttccgggc gatgcggccg ttgccaaaac accgggcgga cagcctgccg
      541 tacagcagga gagcgacgga acggtttaca tttcgctgga tgtcgtaaaa gaatacacag
      601 atctcgacta tgcatattac agcgatccca accgtgttgt aatccggaat gaatgggacg
      661 gcgtggagca ggcgacggta cagtcagaca ccgcgcaggt gcgccagaaa ggcggcatca
      721 agagtctgat tcttgcggat gtacagaagg gtgacaccct gctgtatctg gaaaatctgg
      781 acaactggtg caaagtcatg acggcggacg gctataccgg ctacatccag acagaggata
      841 tctccgagcc ggaggccatc gaagcgcgga cggcaaagaa agattcctat gaaagaatta
      901 cacgggatca caaaatcaat ctggtctggc atcagagcac atcgaccgag tcgaacgacg
      961 cgatggcaga gatgacagca gaaatgaccg gcgtgaacgt gatttcaccg acctggtttt
     1021 ccgtgacaga cgaaaccggt acgatttcca gccttgcgag cgccgattac gtgaaactgg
     1081 cgcatgaagc gggacgggaa gtctggggat tgattgataa ctttaatgaa gcatttgacg
     1141 aaacaaccga tcttgcctac gcatccgtgc gcagcagaat cattgagcag ctgctcgccg
     1201 aggcagcatc gtgcggcatg gacggcatca acgtggattt cgagaatctg aaagaggccg
     1261 gaatcccgca ttacctgcag tttctgcggg aactgacgag cgcggcgcat gcacagaatc
     1321 ttgtggtgtc ggtcgatacg ccggtaccgc aggcttacac gatgtattat cagcgcggcg
     1381 agcaggcaag atttgtggac tatatgattg tcatggccta tgacgagcat ttcgcgggaa
     1441 gcgaggaggc aggctctgtt tcatccctcc cgttcgtaca gcaggccgtg gaggagatga
     1501 cgcgggtgat gcctgccgat caggtgatct gcggaatccc gttttatacc cgtgtctgga
     1561 cggaaaagtt cggacagagc gccattacca gcgaggtgct gggcatggat ggcgccaaaa
     1621 actatgccaa agagaatcag atgacagaaa cctgggatgc atcccttggc cagaatgtgg
     1681 caacggtaga aacttccgat gccagataca cgatctggat ggaggatgag cagtccatgg
     1741 aggaaaaact gaaagtgatc caaagtgccg atctcgccgg agtggcagag tggaagctcg
     1801 gattcgagtg cgcagacgtc tggagtctga tttcagaata tattgaaaca aacagttgac
     1861 gaggagtccg ggaaggataa ggggaatgat aaaagcccgg gcacgaagga ggagtataca
     1921 tgtcagaaaa caatcacgaa gaattattta aaacaacgct gatgggcggt ttcgataagg
     1981 acgatgtcat gacgaaagtg cagaacctga aagatcaggc gtatgccgct cagaagaaac
     2041 tggaagcgaa gatcgaggaa aaagagcagg aaatcgagaa gctgaaccgc aagatccgcg
     2101 agcgtgagga taaaatcgag gagctcgaaa aaaatattca tgagaaatac cagagctata
     2161 tcgacaatta tgacagcatc agcggccttg tgtttgaggc acaggtgaaa gcgaaaaaac
     2221 tgacacagga aacagaagat gcctgccgcg agaaaaccga agagaccgat gcagcgtgca
     2281 gaaagctgac ggaggaaacg gaggagagct gcagaaagct aaaagaagag accgaggaag
     2341 cctgccgccg gaaaaccgaa gaaacggacg agtcctgccg cgagaaaacg gaggaaacgg
     2401 atgaggcgtg ctgcaaaaag agagaggaga caaacgagtc ctgccggaaa cagagggagg
     2461 agacaaagga atcctgtgca aagcagcgcc gcgagacgaa ggaacagtgc gaatacatgg
     2521 tagagagcgc acgtcagaag ggcgaggaac tggctgctgt ttatgaagaa aaactgcagg
     2581 ccgaagcaga agaaatccag agccatatgg acgcggaact gcagaaagcc aacaccagat
     2641 accagaagat ttacgccgag atggaaacca tgacaagtgt actcgagaag aaccgcagaa
     2701 tttttgagga gacttttgcc ggcatcgaaa aaatgcgtga ggagatgaaa cgttctgcat
     2761 cggaagtgga aaccttcatg aaagatccgg taaaaagtat ctttcccgga gaggcaaaag
     2821 aaacagccgc agagccagac cgtgaagata ccgcagaaga agcagaggaa gaagacgaaa
     2881 tcgtcgaaaa ttctgcagtc atcgatctcg aaaatgagcc ggattatctc gatgatgaca
     2941 acgacgaaga agaggatgaa atcccgaatc gcgcgcatga tgaagaagat gaggaagagt
     3001 aaccggaagc aataagagtt gtaacctgcc ccgctgctgc ataaaatgac caagaaagga
     3061 aatttgcagc agggggcttt tcatttggaa aaagggaaaa aactggaatg gctgatggcg
     3121 gtgattctgc tttcggcggc gtatgttctg tcccgcgagg gcgcacggct ggtggcagca
     3181 cagaaggcag ccacaaaagt agtggtgctg gacagcggcc acggcggcga cgatcccggc
     3241 aaaatcgggg caaacggtgt caaagaaaag gacatcaacc ttgcgattgc ccgtctgctg
     3301 aaaaagaagc tggaaaagca gggaatcctt gtggtgatga cgcgggaagg ggaggatgat
     3361 ctctccgatc cgggagccgt gaatgcgaaa gtgcaggatc tgcagcggcg cgtgcagctg
     3421 attcatgaaa aaaagccgga ctgtgtgatc agcattcacc agaacagcta cccggatgcg
     3481 gcggtcaaag gggcgcaggt gttttattat acggattcaa aagaaggcga aaaactggcg
     3541 ctctgcatgc aggctgcact ggtcgcgggg ctcgatccgg cgaatcacag gaaagccaag
     3601 ggaaacaaaa cgtattacat gctgaagaag acagacgcag tgctggtgat ctgcgagtgc
     3661 ggattcctct caaatccgga ggaggaagcg ctcctgaaca caaaggagta ccagaaaaag
     3721 gtggcggatg cactgtgtga cggcgtttta acgtatctgg gagaaaagaa gaatggcaaa
     3781 gagaagacac agacgaagac ggaagagacc gctgcgggcg ctgcttccgc ttatgcttgc
     3841 cctgctggcg gcttatctgg tattcggaga atttgatgag agcgatcccg cgcagaacgg
     3901 aagcggagcg gacagcagca ctgcttcaca gacggcagac gaaaacaatg gtctgtcaga
     3961 aaatggcgca acacagtttc aggcggaaaa tacagatgag gaggagctgg cgaagcggta
     4021 ttattacagc cagctcgatg aaaacagaca gatgatttac cgcgaactgg tgcagggaat
     4081 cgcggaacac caggaaacca tcatcacaaa aggcggcgat cccgatgtga cggcggaggt
     4141 ttacggatgg gtctatatgg attacccgga gtactgctgg atcaacggag catcacatgt
     4201 cacgggatac ggcgagccga agaactactg cgaggtagtt ccggaataca cgattcctgc
     4261 ggaggagatc acaggacgcc agacccagat caaaggggcg ggaaatgatt tcttaagtga
     4321 tatcgaccgt tcgatggatg actatgggaa gatcaaggct gtctttgaaa aatgtatccg
     4381 gcagattgat tatgtgaaag atgcgccgga gaatcagaca ctctacagcg gacttgtgaa
     4441 cggacagacg gtctgcgcag gctatgccag aacgttccag tatctgatga accgcctgga
     4501 catcccggtc atttatgtga cgggaacgac agacaccggc gaggcgcacg gctggaatat
     4561 cgtaaagtgc ggagacaatt attacaacgt cgatgtaacc tggggcgatc cggtattcgc
     4621 agagggcgag agcggcgagt acaatctgcc ggacgacctg atctactacg attttctctg
     4681 cgtttccgat gcggaatttt cggacacgca tcaggcggat gcgaatctgc cgcttccggc
     4741 gtgcaatgca gatgacctcg agtattaccg ccagatcggc cggtacatgg atacatttga
     4801 tgcgacgcag attctgtggg aaatggagac ggatattgaa gagacaaaag agtattccga
     4861 atttaaattt gccaccgatg atctgatggc gcaggtcatg gaagcccgac cggagcttct
     4921 cgataaggct tccggctatc tttgcagcta ttacggactc gggtccgtaa aatatacata
     4981 cagcgaggat acggcgacaa gaaagctgat ggtattctgg caatataaag gagaaccgag
     5041 tgaatgacgg catcatcggc accgcatgcg taggcatcgc gcatcatggt ttccgcctgc
     5101 ggaggcccca tggtcagcac ggtcacggtt ccgccaagtt tttcacggag agaaattgca
     5161 gtttcgagtg caagaagatc atatggattc gtccgtgtat cgccggacat ccgcttcatg
     5221 gcgccggttt ccggatcgat atccactttc gtgccggccg gcacctgttt catacataca
     5281 attatcttca tttctttttc ctcttgtact ttttgacttt gacgcgttaa aacactgtgc
     5341 tgttatagca caaaagtata gggatgtc
//
LOCUS       DAOMUF010000147         5267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4327_length_5267_cov_2.476017, whole genome shotgun sequence.
ACCESSION   DAOMUF010000147 DAOMUF010000000
VERSION     DAOMUF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4327_length_5267_cov_2.476017"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLN77_08305"
     CDS             complement(<1..424)
                     /locus_tag="PLN77_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM44308.1"
                     /translation="MDKKEQASQPALQTLKEYAVITAAVLIMDIGIYVFKFPNNFSFG
                     GVSGMAVVICRFLPFTASQINLFINLLLLLVGFAVLGRNFGVKTAYVTVLSSVLLNIF
                     EKLFPMAHPLTDEIMLELCFAIILPALAAALLFFENASG"
     gene            718..1764
                     /locus_tag="PLN77_08310"
     CDS             718..1764
                     /locus_tag="PLN77_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRM44309.1"
                     /translation="MKNTKVYVDFITEKLKKLLSIDSPTGYTKDAVAWLKAEYEAMGY
                     APVITTKGGLLVQLAKGCEGNGGVLVETHTDTLGGMVAEVKGNGRLRLTALGGMNPNN
                     AETENCVVITRDGKRYEGCFQMNNPSIHVNGSYDQQDRTYDNMEVVLDEMVFSKEETK
                     ALGIETGDIVCFETRTRITEKGYIKSRFLDDKLSVAILLGYAKYLKEENVTLSRPVYQ
                     HITVYEEVGHGGSASVPEGVTDILSVDMGCVGDGLECKEHQVSICVKDSGGPYSYDFT
                     GELIAAAKANGIDYAADVYPHYGSDVEATLRGGADARHALIGAGVYASHGYERSHVDG
                     VKNTLELLAAYLDK"
     gene            1790..3655
                     /locus_tag="PLN77_08315"
     CDS             1790..3655
                     /locus_tag="PLN77_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HRM44310.1"
                     /translation="MRKLALPEEVLLQIEQPARYIGNEFNSVVKDKEKVALRGAFLFP
                     DVYEIGMSHLGIQILYDMFNKREDMWCERVYSPWPDLDRVMREQNIPLFGLESQEPVK
                     NEDFLFITLQYEMCYTNVLQALDLAGIPLHAVERTDEDPIVIGGGPCTYNPEPIAPFF
                     DLFYIGEGEVVYDQLFDTYLENKKNGGTRLDFLKKACQIPGIYVPQFYEVTYHEDGTV
                     AAFTPSIPEAPEKIKKQLVMDMTEATYPEKPVVPFIKATQDRVVLEIQRGCIRGCRFC
                     QAGMVYRPTRERDVEKLKELATHMLRNTGHDEISLSSLSSSDYSHLPELVNYLIDSCP
                     EKGVNISLPSLRIDAFSLDVMSKVQDIKKSSLTFAPEAGSQRMRNVINKGLTEEVILD
                     GAGKAFEGGWNKVKLYFMLGLPTETEDDIKGIAHLAEKIAERYYEIPKDQRNGKCQIT
                     ASSSFFIPKPFTPFQWAPMNTEREFLDKAAIVKAEVRAQLNQKSIRYIYHEADISLLE
                     GFLARGDRKCAEVIEKAYRKGAIFDAWSEYFRKDAWEEAFAETGIDVSFYACRKRSTD
                     EILPWDFLDIGVTKKFLIREWERAQKETVTPNCKMQCSGCGAKSFGGGVCYENQN"
     assembly_gap    3695..3704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3705..4352
                     /locus_tag="PLN77_08320"
     CDS             <3705..4352
                     /locus_tag="PLN77_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HRM44311.1"
                     /translation="VMRYFQKAIRRAELPIAYSEGFSPHMLLSFASPLGVGISSTGEY
                     FDMVLAEDMKTDEIVKRLNATMVEGMEVLSARHVPDGKASKAMSLVAGADYLVQFREG
                     KMPEIDLETKVPEFFALPQIEIMRKTKRSEKLTDIRPWIYDMKAKKSGVWMQLSTGSV
                     SNLKPELVMEAFCKWCGVELPPFAYTILREEVYADIGTEEERKLVPLEALGEEVE"
     gene            4345..>5267
                     /locus_tag="PLN77_08325"
     CDS             4345..>5267
                     /locus_tag="PLN77_08325"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRM44312.1"
                     /translation="MSKRVVIAHLPWEGKDYLVHTLAEGERPVELTAFCREKETLLGN
                     IYVGRVEKLAPGIRGAFVSFAEQQSCFLDLSRVKEGYLLKKAPGTELHVGDEIVVQIQ
                     REAQKTKLPSATAELSLSGSALALYYGKPEISCSGRLLPEQKKRLKELGSRIFPELSG
                     GSLPFGVIFRTASADASEEEIRQEYLELSEKLARIVQYGTMRSLYSCLYQASDAVPEL
                     VKRYAPSVSELVTDDRETYEICREYLERHDFHDCRLTWYEDQLTTLTKKENLENHVKQ
                     ALDKRVDLPSGGFLVIEPTEALTAIDVNSGK"
BASE COUNT         1424 a         1221 c         1454 g         1158 t
ORIGIN      
        1 cgccggaggc attctcaaaa aacagcagtg ctgccgccag tgccggaaga ataatggcaa
       61 agcagagctc cagcatgatc tcatcggtaa gcggatgtgc catcggaaac agcttttcaa
      121 aaatgttcag aagaaccgag gaaagcacgg tcacgtaggc tgtcttgacg ccgaagttcc
      181 gccccagcac agcaaaacca accagcagaa gcagcaggtt gataaaaagg ttgatctgtg
      241 aggcagtgaa cggaagaaac cggcagatga caacggccat accggataca ccgccgaaag
      301 aaaaattatt ggggaattta aaaacataaa ttccaatgtc catgatcaga acagctgcgg
      361 taatgacagc atattctttt aacgtttgta atgcaggctg cgatgcctgt tcttttttgt
      421 ccattttttt gctcctttat ttgaatttga ggcgaccttt attttattat agaagaaact
      481 tttttgaata acagctaata atgtatcccc ctcttcaaac atagcatggc atagggtagt
      541 ttgtccagtt acattttcct tatttccatt tttccgtttt ccgatggttg tatccaattt
      601 atttctgtgg tatgatgaag ggcaggatct tttttcggaa agaaaaaatc tgcagtttct
      661 gtttcaaaat gtttcgaaaa aagaagaaaa aacagaaaca aaaatggagg aagaaacatg
      721 aaaaatacaa aggtttatgt tgattttata acagagaaac tgaaaaaact gctctccatc
      781 gacagcccga ccggctacac gaaagatgcc gttgcatggc tgaaagcaga gtacgaagcg
      841 atgggctatg cgccggtgat caccacaaag ggcggccttc tggtgcagct tgccaaaggc
      901 tgtgaaggaa acggcggtgt ccttgtggag acacataccg atacgctcgg cggcatggta
      961 gcggaagtga aaggaaacgg ccgtcttcgt ctcaccgcgc tcggcggtat gaatccgaac
     1021 aatgcggaga cggaaaactg cgttgtcatc acaagagacg gaaaacggta cgagggctgc
     1081 ttccagatga acaacccgtc gatccatgtc aacggttcct acgaccagca ggaccgcacc
     1141 tacgacaata tggaggtcgt tctggatgag atggtcttct ccaaagagga gacaaaagcg
     1201 ctcggcatcg agactggtga tatcgtctgc tttgagacga gaacccgcat cacggagaag
     1261 ggctacatca agagccgttt cctcgatgat aaactgagcg ttgcaatcct gctcggctat
     1321 gcgaagtatt taaaagaaga aaacgtaaca ctgtcccgcc cggtttatca gcatatcact
     1381 gtatatgagg aggtcggcca cggcggatct gcttccgttc cggagggcgt gacagatatt
     1441 ctttctgttg atatgggctg tgtcggcgac ggactggagt gtaaagagca tcaggtatcc
     1501 atctgcgtca aagacagcgg cggcccttac agctacgatt ttaccggtga gctgattgcg
     1561 gcagcaaaag caaacggaat cgactatgcg gcggatgttt acccgcacta cggctctgac
     1621 gtggaggcaa cgctccgcgg cggcgcagac gcacgccatg ccctgatcgg cgccggtgtg
     1681 tacgcatccc atggatatga gagaagccat gtggacggcg tgaagaatac gctggaactg
     1741 cttgcggcgt atctggacaa ataaaaataa gaacaagtga gagaattgca tgagaaaatt
     1801 agcattgcca gaagaagtgc tgctccagat tgagcagcct gcgcgataca ttggaaatga
     1861 attcaacagt gtcgtaaaag acaaagagaa agtagccctg cgcggcgcat tcctgttccc
     1921 ggatgtgtat gagattggta tgtcccatct tggcatccag atcctgtacg atatgttcaa
     1981 caaacgcgag gacatgtggt gcgagcgagt ttattcccca tggccggatc tggaccgcgt
     2041 gatgcgtgaa cagaacattc cgctgttcgg tctggaatcc caggagccgg tgaaaaacga
     2101 ggatttcctg ttcatcaccc tgcagtatga gatgtgttat acaaacgttc tgcaggcgct
     2161 cgaccttgcc ggaatcccgc tgcatgcggt ggagcgtacc gacgaggacc cgatcgtcat
     2221 cggcggcggc ccgtgtacgt acaacccgga gccgatcgct ccgttcttcg atctgtttta
     2281 catcggagag ggtgaggttg tgtacgacca gcttttcgac acgtatctgg aaaataaaaa
     2341 gaacggcgga acccgtctgg attttctgaa aaaggcatgc cagatcccgg gcatttacgt
     2401 accgcagttc tatgaagtaa cgtaccacga ggacggaacc gttgcagcct ttacgccgtc
     2461 catcccggaa gcgccggaaa agatcaaaaa acagcttgtc atggatatga ccgaggcaac
     2521 gtatccggag aaaccggtcg tgccgtttat caaggcaaca caggaccgtg tcgttctgga
     2581 gatccagcgc ggctgtatcc gcggctgccg tttctgccag gcgggtatgg tgtaccgtcc
     2641 gacaagagag cgcgatgtgg agaagttaaa agaacttgcg acccatatgc tgcgcaatac
     2701 cggccacgat gagatttcgt tgagttcttt gagctcgagt gattacagcc atcttccgga
     2761 gcttgtcaac tatctgatcg acagctgccc ggaaaaaggt gtcaatattt cccttccgtc
     2821 tcttcgaatc gacgcatttt ccctcgatgt catgagcaag gtacaggata tcaaaaagag
     2881 cagcctgacc ttcgcaccgg aggcgggaag ccagcggatg cggaatgtta ttaataaagg
     2941 tctgaccgag gaagtcattc tcgacggagc cggaaaagcg tttgagggcg gctggaataa
     3001 agtaaaactg tacttcatgc tgggccttcc gaccgagacc gaggatgata tcaaaggaat
     3061 tgcgcatctg gcggaaaaga tcgcagagcg ttactatgag attccgaaag accagagaaa
     3121 cggaaaatgc cagattacgg caagctcctc gttctttatt ccaaagccgt tcaccccgtt
     3181 ccagtgggca ccgatgaaca cggagaggga gtttcttgac aaagcggcga ttgtaaaggc
     3241 agaggtccgc gcacagctga atcagaagag catccgttac atttatcatg aggcagatat
     3301 ttcccttctg gagggattcc ttgcacgcgg cgatcggaaa tgtgccgaag ttatcgaaaa
     3361 agcgtaccga aaaggtgcta ttttcgatgc ctggtccgag tatttccgga aagatgcctg
     3421 ggaagaagcg tttgcagaga ccggcattga cgtttcgttc tatgcctgcc gtaagcgcag
     3481 caccgatgag atccttccgt gggatttcct tgatatcggc gtgacgaaaa aattcctgat
     3541 ccgcgaatgg gagcgggcac agaaagaaac cgttaccccg aactgtaaga tgcagtgttc
     3601 cggctgcgga gcaaaatcat tcggaggagg tgtctgctat gaaaatcaga attaagtttg
     3661 ccaaaacagg tgtgatgaaa tttgtgggtc atctnnnnnn nnnngtgatg cgttacttcc
     3721 agaaagcaat ccgccgtgca gaacttccga tcgcttattc cgagggcttc agcccgcaca
     3781 tgctgctgtc gtttgcttct ccgctcggtg tcggcatcag cagcaccggt gaatatttcg
     3841 atatggtgct cgcggaagat atgaaaaccg acgagattgt aaaacgtctg aatgcgacga
     3901 tggttgaggg aatggaagtg ctttccgccc gccatgtgcc ggatggaaaa gcaagcaagg
     3961 caatgtctct cgtagcagga gccgattatc tcgtgcagtt ccgtgaggga aaaatgccgg
     4021 agatcgatct ggaaacgaaa gtgccggagt ttttcgcact tccgcagatt gagatcatgc
     4081 gaaagacaaa acgaagcgaa aagctgaccg atatccgccc atggatttac gatatgaaag
     4141 caaagaaaag cggtgtctgg atgcagctct ccaccggaag tgtttcgaac ctgaaaccgg
     4201 agctcgtgat ggaagcgttc tgcaaatggt gcggggttga actgccgccg tttgcataca
     4261 cgattctgcg cgaggaagtt tacgcagaca tcggaacgga agaagagcga aagctggttc
     4321 cgctggaagc attaggagaa gaagttgagt aaacgagttg tcattgccca tctgccgtgg
     4381 gagggaaaag attacctggt acacacgctg gcggaaggag aaagaccggt cgaactgacg
     4441 gccttctgca gggagaagga aacgctgctt ggcaatatct atgtcggccg ggtggaaaaa
     4501 ctggcgcccg ggatccgcgg ggcatttgtc tcgtttgcgg agcagcagag ctgttttctt
     4561 gatctgtccc gtgtaaaaga agggtatctt ctgaaaaaag caccgggaac ggaactgcat
     4621 gtcggggatg aaatcgtcgt gcagatccag cgggaagcgc agaaaacgaa gcttccgtcg
     4681 gcgacagcag aactttctct ttcgggaagt gccctggcgc tctattatgg aaagccggaa
     4741 atctcatgct cgggacgcct tctgccggag cagaaaaaac ggttaaaaga actgggaagc
     4801 cggatctttc cagagctttc cggcggaagc cttccgtttg gcgtgatctt ccgcaccgca
     4861 tccgctgatg cttcggaaga agagatccgg caggaatatc tcgagctgtc cgaaaaactc
     4921 gcgcggattg tccagtatgg aacgatgcgg agcctgtact cctgcctgta tcaggcatcg
     4981 gatgccgtgc cggagctcgt caaacggtat gcaccgtcgg tttcagaact tgtgacagac
     5041 gacagagaaa cgtatgagat ctgcagagaa tatctggagc gccatgattt tcacgactgc
     5101 cgcctgacct ggtatgagga tcagcttacc acactgacaa agaaagaaaa tctcgaaaat
     5161 cacgtaaaac aggcactcga caaacgtgtg gatctgcctt ccggcggatt cctcgtgatc
     5221 gaaccgaccg aagcgctgac cgcgattgat gtgaacagcg gaaagca
//
LOCUS       DAOMUF010000148         5065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4565_length_5065_cov_2.764072, whole genome shotgun sequence.
ACCESSION   DAOMUF010000148 DAOMUF010000000
VERSION     DAOMUF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4565_length_5065_cov_2.764072"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1571)
                     /gene="acsC"
                     /locus_tag="PLN77_08330"
     CDS             complement(216..1571)
                     /gene="acsC"
                     /locus_tag="PLN77_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HRM44313.1"
                     /translation="MALSGIQIFKMTPKKNCKECGCPTCMAFSMKVAQGAMDISACPY
                     MSDDAMAQLSEATAPPMKTIKVGTGDSEYTLGGETVLYRHEKTFVSKTRYAVSLCSCM
                     SDEEIDAKLEEDKKVDYDRIGERMCAEMLYVNYAGNGVDKYVSLVTKAAATGKVLVLG
                     CEDAEAAKAALEVCKAGKPILNGANASNYEEMSKLATEAGVVLGVSGANIDELHDTVE
                     AIEKLGNKNLVLDTTEATIKETFATTVQVRRASLKDTDRTFGYPSIVNLAKIAQGDRY
                     MQQALLSLFTMKYGSIIVLEEMGYAEALPVFGLRQNVFTDPQKPMKVEPGIYPLNGAD
                     ENSICLTTVDFALTYFLVSGELERSGVPVNLVINDAGGLSVLTSWAAGKFSGNSISKF
                     FKENVEDKVKSRKVIIPGKVAVLKGDIESKLPGWEVIVAPLEAVQLVKFMKDLQANGE
                     L"
     gene            complement(1609..2550)
                     /gene="acsD"
                     /locus_tag="PLN77_08335"
     CDS             complement(1609..2550)
                     /gene="acsD"
                     /locus_tag="PLN77_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HRM44314.1"
                     /translation="MPFNRKPQKFNAAIKSVVIGSGDKTVTLGGENVLPFYAFDGEIK
                     NGPKVGVEITDLGMEGEPESVKAYYEGAATMGEIAKKAAAMEGADFLCLRLAGGDPNG
                     LNKSVEELIETVKEVADAVDVPLVVEGCKNVEKDSELLTKVAEVLQGRNVLVMSAREE
                     DYKAVGAAAGLAYNQKVGAESAVDINLAKQLNVVMTQLGVSADSIVMNVGSAAVGYGY
                     EYVVSTLDRIKAAALSQDDKMLQMPIITPIASETWNVKEAMATEEESPEWGNQEVRGV
                     SMEIQTAAASLASGSDAVILKHPQSVATISKMIQELM"
     gene            complement(2849..4978)
                     /gene="acsB"
                     /locus_tag="PLN77_08340"
     CDS             complement(2849..4978)
                     /gene="acsB"
                     /locus_tag="PLN77_08340"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HRM44315.1"
                     /translation="MTLFERVFGGNDAVYGLTEAAVDAAIAANGEEKAVSFPDTAYAL
                     PCYYAVTGVKVTNLKEMKEALGVVKSLMTRENQLNDVFMSGVATALCAEFIEAIKYID
                     GAKPYDEPCYGHLSDAIIRELGVPLVTGDIPGVAVILGSAPTAQEGVDLVKSYQAQGI
                     LVTLVGGIIDQCQELGYKTGANVRVIPLGKDVTSVIHAVSVAIRAALIFGNVTAGDAG
                     ALMTYTKDRVPAFVNAFAPLDDVIVACGAGAIALGFPVITNDDKDVPAIPKSLIIQKD
                     VSKFNATSLEARDIKIKITNIDIPVAFASAFEGEIIRRGDMQVEFDGSRVDCCELVQT
                     KDASEVEDHKIELIGPDIDEFEVGSKHSIAYVVEVAGKSMQPDFEPVFERKFHSYLNC
                     IEGVMHTGQRDMIRIRVSKEAFNAGFRAKHFGEVLYAQVKNEFEAVVDKCQVKIYTNP
                     EDCTKIRHEIAIPVFDKRDERLSTMTDESVPVYYSCIMCQAFSPSHVCVVTPERLGLC
                     GAVSWLDAKATNELDPNGPCQVITKEKVIDERIGEYEDVNEAVRKLSQGALDDVSLYS
                     IMEKPMTSCGCFECICGIEPFSNGVCIANREYAGMTPLGMTFSELASMTGGGVQTPGF
                     MGHGKHFIASKKFMKAEGGVARIVWMPKELKEQVAEKLNETAKELYGIDNFTDMIGDE
                     TIAEDPETLVAFLTEKGHPALGMDPMM"
BASE COUNT         1173 a         1296 c         1093 g         1503 t
ORIGIN      
        1 ccagcatcca gctgctgttt tgctctcttt aaaattggct cttcatctct tgctgcgaat
       61 gctttgttta cagtaggagc tgtaacagaa agtctttcgc cgatggttac aaattttgcc
      121 atatctcttc tccttttctt ttgggaatcc cccataatta ttttttcacg ggagaaactt
      181 ccggataccg gagtttctcc cggatttttt atgtattaca gttctccgtt tgcctgaaga
      241 tctttcatga atttcacaag ctgaactgcc tcaagcggag caacgattac ttcccatcca
      301 ggaagtttgg actcaatgtc gccctttaat acggctactt taccaggaat gataactttt
      361 ctggatttta ctttatcttc tacattctct ttgaagaatt tggagataga gtttccggag
      421 aatttacctg ctgcccagga tgtcagtaca gaaagtccgc cggcatcgtt gattacaagg
      481 ttaaccggaa ctccggatct ctcgagctct ccggatacca ggaaataggt cagagcaaag
      541 tctactgtgg tcaggcagat cgaattttca tctgcgccgt tcagcgggta gatgcccggc
      601 tctactttca tcggtttctg cggatcggta aatacgttct ggcgaagtcc gaataccgga
      661 agtgcctctg cgtatcccat ctcctcaaga acgatgatag aaccgtattt catggtgaac
      721 agggaaagca gtgcctgctg catatatctg tcgccctgtg cgatttttgc cagatttaca
      781 atagacggat aaccgaaggt acggtctgta tcctttaagg atgcacgtct tacctgtacg
      841 gtagtagcga aggtctcttt gatggtcgct tctgtggtat caagaacaag gttcttgttg
      901 ccgagtttct cgattgcttc tacggtgtcg tgaagctcat caatgtttgc gccgctaacg
      961 ccgagaacaa cgcctgcctc tgtggcaagt ttgctcatct cttcataatt ggatgcattt
     1021 gcaccattca ggatcggttt gcctgctttg catacttcca gagcagcttt tgcagcttct
     1081 gcgtcctcac agccaagtac cagaactttt ccggttgctg ctgctttcgt tacaagagat
     1141 acgtatttgt ctacgccatt tccggcatag tttacataca gcatctctgc gcacatacgc
     1201 tcaccgatac ggtcgtaatc aacttttttg tcttcttcca gttttgcatc gatctcttcg
     1261 tcagacatgc agctgcagag agataccgca taacgggttt tgcttacaaa tgttttctca
     1321 tgacgataga ggaccgtctc tccgccaaga gtgtactcac tgtcgccggt tccgactttg
     1381 atcgtcttca ttggcggtgc ggtagcttcg gaaagctgtg ccatagcgtc gtcagacata
     1441 tatgggcacg ctgaaatatc catagcgccc tgtgcaacct tcatggagaa tgccatacag
     1501 gtcggacatc cgcactcctt acagtttttc tttggggtca ttttaaaaat ctgaatacct
     1561 gataatgcca taattcttta tcctcctaat ctcttcctta cgtactgatt acatcagttc
     1621 ctgaatcatt ttggaaatgg ttgcaacaga ctgtggatgt ttgaggataa ctgcatcaga
     1681 accagaagcc agagatgcgg ctgctgtctg aatctccata gagactcctc gtacttcctg
     1741 atttccccat tccggagatt cctcttctgt tgccattgcc tcttttacat tccatgtctc
     1801 agaagcgatt ggagtaataa ttggcatctg cagcatttta tcgtcctggg ataatgctgc
     1861 tgctttgata cggtccagtg tagatactac gtactcatat ccgtatccga ctgctgcaga
     1921 acctacgttc ataacaatgc tgtctgcgct gacaccgagc tgggtcataa caacattcag
     1981 ctgttttgca aggttgatat ctacagcgga ctcagcgccg actttctggt tgtatgccag
     2041 acctgcggct gcaccaactg ccttgtaatc ttcttctctt gcggacataa caagaacgtt
     2101 tcttccctga aggacctctg ctacttttgt gagcagttcg gaatccttct caacgttttt
     2161 acatccttct acaacaagcg gcacgtcaac ggcatcagca acctctttga ctgtctcaat
     2221 gagttcttct accgatttgt tcagaccgtt cgggtctccg cctgccagac gaaggcagag
     2281 gaaatctgca ccttccatag ctgctgcttt ttttgcaatc tcgcccatgg tagctgcacc
     2341 ttcatagtat gcttttacgc tttccggttc tccttccata ccaaggtctg tgatttcaac
     2401 gccgaccttc ggtccgtttt tgatttcacc gtcgaaagca tagaatggta atacgttttc
     2461 tccgccaaga gttacggttt tgtctccgct gccgattaca acggatttaa ttgctgcatt
     2521 aaacttttgt ggtttgcgat taaacggcat aaaaaaagcc tccttaaaaa taaaattcca
     2581 tacaggcata tctccatgat tccgcttcat ggacacacct gttattattt taacgttttt
     2641 tcccggagaa tgcaagattt accaggatat tttccaagat tttctgacaa aaagtatccg
     2701 ttttcttttc gtcagccgtt ttttcccaga aaatcacttt acagctgccg gcatgtgccg
     2761 gaaagggctt cctcctccgc tttggaatcc cctcccaagc catgccggtc tgcttattct
     2821 ttcacaggat aagcctgtaa aatacgaatt acatcattgg atccatgcca agtgccggat
     2881 gacctttctc ggtcaggaat gcaaccagtg tctccggatc ttctgcgatg gtctcatcac
     2941 cgatcatatc ggtaaagttg tcgataccgt acagctcttt tgctgtttca ttcagtttct
     3001 ctgcaacctg ctctttcagc tctttcggca tccatacgat acgggcaaca ccgccttctg
     3061 ctttcataaa cttcttggaa gcgataaagt gtttgccgtg tcccatgaat ccaggagtct
     3121 gaacaccgcc gcctgtcatg gaagccagct cggagaaggt cattcccaga ggagtcatgc
     3181 ctgcgtactc acggtttgcg atgcagacac cgttggagaa cggctcgata ccacagatac
     3241 actcgaagca tccgcaggat gtcattggtt tttccatgat ggagtacaga gatacatcat
     3301 caagagcacc ctgggaaagt ttacgaacag cctcgttaac atcctcgtac tcaccgattc
     3361 tctcatcgat tactttttct tttgtgataa cctggcaagg tccgttcgga tccagctcgt
     3421 tggtagcttt tgcatccagc catgaaacag caccgcaaag tccgagacgc tctggtgtaa
     3481 caacacatac atggctcggg gagaatgcct ggcacatgat acagctgtaa tataccggca
     3541 cggattcatc tgtcatggta gacagacgct cgtcacgttt gtcgaatacc gggattgcaa
     3601 tttcgtggcg gatcttggta cagtcttccg gattggtgta gatcttaacc tggcatttgt
     3661 caacaacagc ctcgaactcg ttcttcacct gtgcgtacag aacttctccg aagtgttttg
     3721 cacggaagcc tgcgttgaaa gcttctttgc tgacacggat acggatcata tcacgctgtc
     3781 cggtatgcat cacaccttcg atgcagttca ggtagctgtg gaatttacgc tcaaatacag
     3841 gctcgaaatc cggctgcatg ctctttccgg caacctcaac aacgtatgca atgctgtgct
     3901 tggaacctac ctcgaactcg tcgatgtccg gtccgatcag ctcgatcttg tgatcttcta
     3961 cttcggaagc atcttttgtc tgaaccagct cgcagcagtc tacgcgggaa ccgtcaaact
     4021 caacctgcat atcaccacgg cggatgattt caccctcgaa tgcagatgcg aatgcaaccg
     4081 ggatatcaat gttggtgatt ttgatcttga tatcgcgtgc ttccagagaa gttgcgttga
     4141 atttggaaac gtccttctgg ataataaggc tcttcggaat agccggaacg tctttgtcat
     4201 cgtttgtgat aaccggaaag ccaagtgcaa ttgcaccagc gccgcatgca acgatcacat
     4261 catccagagg agcaaaagca tttacgaatg ccggtacacg gtctttggtg taggtcatca
     4321 gtgcgcctgc atcgcctgca gttacgttac cgaagatcag ggctgcacgg attgctacgg
     4381 atacagcatg gataacagat gttacgtctt ttccaagcgg gatgacacgt acgtttgcgc
     4441 cggtcttgta tcccagttcc tgacactgat caatgattcc gccaaccagt gttacaagaa
     4501 taccctgtgc ctgatagctc ttaaccagat ccacgccttc ctgtgcagtc ggagcgcttc
     4561 caaggataac agctacgccc gggatatcgc ctgttacaag cggcacacca agctcacgga
     4621 tgatggcatc gcttaaatgg ccgtagcacg gctcatcata tggttttgca ccgtcgatgt
     4681 atttaattgc ctcgatgaac tctgcgcaga gtgcagttgc aacaccggac ataaatacat
     4741 cattcagctg attttctctt gtcatcagag attttaccac gccaagagcc tctttcatct
     4801 cttttaagtt ggtaaccttt actcctgtta cagcatagta acacggaagt gcgtaagcag
     4861 tgtccgggaa gctgactgct ttttcctctc cgtttgctgc gattgcggca tcaacggcag
     4921 cttcggtaag accgtatact gcatcatttc cgccaaatac tctctcgaat aatgtcatgt
     4981 gtttttcctc ctatcacact ttttccatgc aaggcatgtt tgacttatcg atttttgttt
     5041 ttattttccg tatctcttct actgc
//
LOCUS       DAOMUF010000149         5025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4630_length_5025_cov_2.695372, whole genome shotgun sequence.
ACCESSION   DAOMUF010000149 DAOMUF010000000
VERSION     DAOMUF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4630_length_5025_cov_2.695372"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLN77_08345"
     CDS             complement(<1..582)
                     /locus_tag="PLN77_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRM44316.1"
                     /translation="MTLKELPIGKTATVRTVGGEGALRQHFLDMGIIPGAEVTMVKYA
                     PMGDPVEVRIHSYELTLRLADAGRIAIDEMRDAVKEKEQPDAKAIPHPGFGEGGKYHN
                     KAEEHPLPEGELLSFALAGNQNCGKTTLFNQLTGSNQHVGNFPGVTVDRKDGEIRGQK
                     NTLVTDLPGIYSMSPYPSEEIVTRNFVLNEHPRG"
     gene            complement(659..2188)
                     /locus_tag="PLN77_08350"
     CDS             complement(659..2188)
                     /locus_tag="PLN77_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRM44317.1"
                     /translation="MNRGITQKQYLELVPPTEEDARSSQMRILDALKEKGITASFTLP
                     ALQKLYPICDEADYNITVSLAWNGSIWQAVDLEAGDTAAEHYGYAADLGSTTVVVRLV
                     NCSDGTVLAEESEYNRQTAYGTDILTRIFACKDKPEVLQDIRACTMETFTLLFEKLRQ
                     KTGIAPSDVLSMVVSGNTTMIHFLLGLDPFCVFSSPYAVRADRVGFLPAKEMGFPMKG
                     CVYCVPGKSNYLGGDILSGMIATELYKKETISVFFDIGTNGELVVGNREFLLCGAGAA
                     GPALEGGVVKTGMRAAEGAVDTVKIEDGKIQCHVIGEGKPKGICGSGIIDLLAELFLN
                     GWINLFGTLQPERSEKIKEDPETGEWCVEYSEGLCFYQSDIAEFLRTKSAAYTMVEYM
                     MRESGISMEEIDRFYAAGAFGKHVSKESAITIGMYPDMDRSRLICAGNTSLAGAEKIL
                     LDRTCIDDIDRILDEMVYIQFGQVAAFISMMKAAQAIPHTDLELFPSVQKKLEEKQKE
                     K"
     gene            2402..3880
                     /locus_tag="PLN77_08355"
     CDS             2402..3880
                     /locus_tag="PLN77_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRM44318.1"
                     /translation="MMDYRNNYNNENETNNENMMAAEQNSFNGKKPSSKNKKAAKLAK
                     KIGAIALSAVLFGGVAGGTFQAVNHFAGSTTATATTTTAQQTTSNSSLLKAAATSSSS
                     TSTGTMDVSTIAKNAMPSIVSITNQSVQEVQNYFSMFGYGAQTPQTEETTSCGSGIII
                     GKNDTELLIVTNNHVVEDADTLSVSFVNNQVCKANIKGTDADNDLAVIAVPLSEIPDD
                     TMSSIAVATMGDSDSVEVGEQVVAIGNALGYGQSVTTGIISAANRVIDSDSSSDGSSD
                     GTTADTSSTDTTGKTYLQTDAAINPGNSGGALLNMNGEVIGINSAKLASTEVEGMGYA
                     IPVSRVSDIIENLMNEQTRTKVAAEDQGTIGIKCLDVSSQIQQAYNMPAGIYISEVTS
                     GGAAENAGLKSGYVLTSFDGHSITSTSELKSLLQYYSAGETVEVEVQVPDNGSYETKT
                     FSLTLGTSISSSDDQQSDSQQNAGSSRSAEGGNTLTSATPSI"
     gene            complement(3999..>5025)
                     /locus_tag="PLN77_08360"
     CDS             complement(3999..>5025)
                     /locus_tag="PLN77_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRM44319.1"
                     /translation="MLGCNIGCTMSAILASFGCKKDAKRAACVHLLFNISGTIVCSII
                     FLLFGNQVVDFFMGISGNEAGRMIANANSIIKVCQVLLMLPFTPLLVKATYFIIRGND
                     EEDKKFELAYISSKHAMSPTTAVLQVVREMERMAQMAETNLIRAMNTLVTRDQKEIDE
                     VYRVEENINFLNKEITNYLVHLNQASLPTSDVMRIGALFHVVNDIERIGDHAENVADS
                     AVQMTNDNVTFSKQGELDLSEMLDMVLKILDESIEMFAKNDLQHLQEIIDIENSIDQE
                     ERDLQQKHVERLTRNECTPEAGMIFSDLVSGLERVADHATNIAFSILDEDPEEKAARE
                     AVAGAEK"
BASE COUNT         1221 a         1397 c         1149 g         1258 t
ORIGIN      
        1 gccgcgcgga tgctcgttca gaacgaaatt tcgcgtgaca atctcttcac tggggtacgg
       61 cgacatggaa tagataccgg gaaggtccgt gaccagcgtg ttcttctgac cgcggatttc
      121 accatccttc cggtccaccg tcacgcccgg gaaatttccg acatgctgat tcgatcccgt
      181 cagctggttg aacaacgtag tcttgccgca gttctggttt cctgccagtg caaaggacag
      241 cagctctcct tccggcagtg gatgctcctc tgccttattg tggtactttc cgccctctcc
      301 aaagccggga tgcggaattg ctttcgcatc cggctgctct ttttctttta cagcgtcccg
      361 catctcatca atggcaatcc gtccggcatc ggcgaggcgg agtgtcagtt catagctgtg
      421 aatccgcact tccacagggt ctcccatcgg tgcatacttt accatcgtca cttccgcccc
      481 cggaatgatt cccatatcta ggaagtgctg ccgcagcgcg ccctctcctc ctaccgtgcg
      541 gacggtcgct gttttgccga tcggcagttc tttcagtgtc atatatttca ctccttattg
      601 attctcatta tcaatagtaa gtatatgaca caattcgact gcggtcaagc aattgccatt
      661 atttctcttt ttgtttttct tcgagcttct tctgtacact cggaaacagc tccagatccg
      721 tgtgtgggat tgcctgggcc gctttcatca tagagatgaa agcggcgacc tggccgaact
      781 ggatgtagac catctcgtcc agaatgcggt caatatcgtc gatgcaggtc cgatccagca
      841 ggattttttc tgctcccgca agcgacgtat ttccggcgca gatcaggcgg ctgcggtcca
      901 tatccgggta catgccgatc gtgatggcgg attcctttga aacgtgcttt ccaaatgcac
      961 cagccgcgta aaaccggtca atttcttcca tggaaatgcc ggattcacgc atcatgtact
     1021 caaccatcgt gtaggctgct gactttgtac gaaggaattc ggcgatatca ctctgataga
     1081 agcacagccc ttcactatat tcaacgcacc actctcccgt ctccgggtct tccttgatct
     1141 tttcggaccg ctccggctgc agggtgccga acaggttgat ccatccattt aggaataatt
     1201 ccgcgagcag atcgataatg ccggaaccgc agatgccctt tggcttaccc tctccgatta
     1261 catggcactg gattttcccg tcttctattt ttaccgtatc gacagcgcct tctgccgctc
     1321 gcatgccggt tttgaccacg ccgccctcga gcgccggacc ggcagcgcct gcgccgcaga
     1381 gcagaaattc ccggtttccg acgacaagct cgccgttcgt tccgatatca aaaaaaacgc
     1441 tgatcgtttc ttttttatag agttccgttg cgatcatacc gcttaatata tcaccgccca
     1501 ggtaattgga ttttcccggc acgcagtaga cacatccctt catgggaaat cccatctctt
     1561 tcgccggaag gaatccgacg cggtccgcgc gcacggcgta cggggatgaa aagacgcaga
     1621 acggatccag tcccagcagg aaatggatca tcgttgtgtt gccggatacg accattgaga
     1681 gaacatccga cggcgcaatt cctgtctttt ggcgaagttt ttcaaagagc agtgtaaatg
     1741 tctccatcgt acacgcacgg atgtcctgca gtacctccgg tttatcctta caggcaaaga
     1801 ttctcgtcag aatatcggtt ccataggcag tctggcggtt gtattcgctc tcctcggcaa
     1861 gcacggtacc gtcgctgcag tttaccaggc gcacaacgac ggtcgtactt ccaagatcag
     1921 cggcatagcc gtaatgctct gctgctgtat cgcccgcttc cagatcaacc gcctgccaga
     1981 tgcttccatt ccatgcgagg gaaaccgtta tgttatagtc tgcctcatcg cagatgggat
     2041 acagtttctg cagcgctgga agggtaaatg atgcggtgat tcctttctct tttaatgcat
     2101 ccagtatccg catctggctg gagcgggcgt cctcctccgt cggcgggacc aactccagat
     2161 actgtttctg tgttattcct ctattcatta ttacttcctc atttcctgat catttttctg
     2221 tttcttattg tagcactttt ctcttttcca gagaacttat ttttttcaca tttttactac
     2281 atttccatca cagattccca ttatactgca aagcatagaa acaaaaaagg aagcaaacaa
     2341 aaccggaagc cgattccgga acacagttta aaatacagat accagaaagg aagtatttat
     2401 tatgatggac tacagaaaca actacaataa cgaaaacgaa accaataatg agaacatgat
     2461 ggcagcagag cagaacagct tcaacggaaa gaaaccttct tccaaaaata aaaaagctgc
     2521 taagcttgca aagaaaatcg gagccatcgc cttaagcgca gttctgttcg gcggtgttgc
     2581 cggcggtacc ttccaggcag tcaaccactt tgccggaagc acgacagcca ctgcaacaac
     2641 aaccactgca cagcagacga cttccaacag cagcctctta aaagccgctg ccacatcctc
     2701 aagctccact tccaccggaa cgatggatgt ttccacgatt gcgaaaaacg caatgccgtc
     2761 gatcgtctcc atcacaaacc agtccgtaca ggaagtacag aactacttca gcatgttcgg
     2821 ttacggtgcc cagactccgc agaccgagga gaccacaagc tgcggttccg gtatcatcat
     2881 cggaaagaat gacaccgagc ttctgatcgt caccaataac catgtagtag aagatgccga
     2941 tactctttct gtcagttttg taaacaatca ggtctgcaag gcaaatatca aaggtaccga
     3001 tgccgataac gatcttgccg tcatcgccgt tccgctctcc gagatcccgg atgacacgat
     3061 gagcagcatc gctgttgcca cgatgggcga ttctgattcc gttgaggtcg gcgagcaggt
     3121 cgttgcgatc ggcaacgcac tcggctacgg ccagtccgtt acgaccggta tcatcagtgc
     3181 tgcaaaccgt gtgatcgaca gcgattcctc ttctgacgga agctccgatg gaacaacggc
     3241 agatacttct tccactgata caaccggaaa gacctactta cagaccgatg cagccatcaa
     3301 ccctggcaac agcggcggcg ctcttctgaa catgaacgga gaggtcatcg gtatcaactc
     3361 cgcaaaactt gcttctacag aggtagaggg catgggctat gcaatcccgg tctcccgtgt
     3421 ttccgatatc attgaaaatc tgatgaatga gcagaccaga acaaaggttg ccgcagaaga
     3481 tcagggaacg atcggcatca aatgcctcga tgtcagcagc cagatccagc aggcatacaa
     3541 tatgccggcc ggcatctata tctccgaagt aacctccggc ggtgccgctg aaaatgcagg
     3601 tctgaaatcc ggatacgttc tgaccagctt tgacggacat tccatcacct ccaccagcga
     3661 gctgaaatcc ctgctgcagt attactctgc cggcgaaacc gtcgaggtgg aagtacaggt
     3721 tccagacaac ggatcctatg agacaaagac cttctccctg accctcggaa cgagcatcag
     3781 ctcttcagat gatcagcaga gcgattccca gcagaatgcg ggttcatccc gctctgcaga
     3841 gggaggaaat accctgacct ccgctactcc ttcgatctga ctgcccgctc tcccgtgaaa
     3901 gaaatccata aataaaaaac atcataagca tgaaagaaag gccccgaaac ataactccaa
     3961 tgtttcgggg cctttctcct attctcttaa tccatttctt atttttctgc tcctgcaact
     4021 gcctcgcggg ctgctttctc ttccggatct tcatccagaa tggagaatgc aatattggtt
     4081 gcatggtccg cgacacgctc gagtccggat acaagatcgg agaagatcat gccggcttcc
     4141 ggcgtacatt cgttgcgggt cagacgctct acatgcttct gctgcagatc ccgctcctcc
     4201 tgatcaatgg aattttcaat atcaatgatc tcctgcaggt gctgcagatc attctttgca
     4261 aacatttcga tcgactcatc gagaattttt aataccatat ccagcatctc ggaaagatcc
     4321 agttcgccct gtttgctgaa ggtcacattg tcattcgtca tctgcacagc agaatctgcg
     4381 acattctcgg catgatcacc aatccgctcg atatcattga ccacatggaa cagcgcaccg
     4441 atgcgcataa cgtctgatgt cggcagcgat gcctgattta agtgaaccag atagttcgta
     4501 atctctttgt tcaggaaatt aatattttcc tcgacacggt agacctcgtc gatttctttc
     4561 tgatcgcggg tcaccagcgt attcatcgcg cgaatcaggt tggtctctgc catctgtgcc
     4621 atacgctcca tctcacggac aacctgcaga actgcagtcg tcggggacat cgcatgtttc
     4681 gagctgatgt aggcaagctc gaactttttg tcttcctcgt catttccgcg gatgataaag
     4741 tacgttgcct tcaccagaag cggggtaaac ggcagcatca ggagtacctg gcatactttg
     4801 atgatcgagt ttgcatttgc gatcatacgg cctgcttcgt ttccggaaat acccataaag
     4861 aagtcaacta cctgatttcc aaacagcagg aaaataatgg agcagacaat cgttccggaa
     4921 atgttgaaca acaggtggac acatgccgca cgttttgcat cttttttgca gccgaagctg
     4981 gcaagaatag cggacatcgt acatccgatg ttgcatccaa gcatc
//
LOCUS       DAOMUF010000150         5010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4646_length_5010_cov_3.036932, whole genome shotgun sequence.
ACCESSION   DAOMUF010000150 DAOMUF010000000
VERSION     DAOMUF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4646_length_5010_cov_3.036932"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..322)
                     /locus_tag="PLN77_08365"
     CDS             complement(29..322)
                     /locus_tag="PLN77_08365"
                     /inference="COORDINATES: protein motif:HMM:NF019488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HRM44320.1"
                     /translation="MRKKKKQKKSLHPPGGELLSAMQVPSDLAFGEPVVTFYGGREVQ
                     IENYRRILEYNACRLCVLTQYGRLLLVGEGIRIVSYSGEEMRVEGRIDQVTFQ"
     gene            complement(453..596)
                     /locus_tag="PLN77_08370"
                     /pseudo
     CDS             complement(453..596)
                     /locus_tag="PLN77_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(1281..1820)
                     /locus_tag="PLN77_08375"
     CDS             complement(1281..1820)
                     /locus_tag="PLN77_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HRM44321.1"
                     /translation="MTQTEIEEYITQFPIYQYAFVKPEDIEFNDRVRQICKKECSRYG
                     ASWSCPPAVGTVEVCKERCMNYDHVLFFSSIAEVDNIMDMDETLKTKSEHEKITREIE
                     QHLKDKTLLTFALSSDSCQLCDKCTYPRGGCRHLELMHPCIESHGIVVANLIEKNMMD
                     YYLGEHMVIWFSMIFYKES"
     gene            complement(1842..2822)
                     /locus_tag="PLN77_08380"
     CDS             complement(1842..2822)
                     /locus_tag="PLN77_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM44322.1"
                     /translation="MRTRVLQEKNYAERMQNGVEPYLAAHAEEFYWERESGHPIHVLR
                     YRAFRPRGVVVISHGFTENAEKYKEIVYYFIKEHFHVYLPEHCGHGKSYRLVEDPSLV
                     YVDSYHRYVEDLLFVARKAKAEACGLPLVLYSHSMGGGIAAAAAAKAPHLFRKVVLSS
                     PMIRPLTGKVPFADAKRIAAFACATERGTQYVVGQKPYQGIESFKQSSSLSRPRFLYY
                     QQKREKEPLYHLNAASYGWLHAAAHLYRDLMRSGWKRIEAPVLLFQAENDHLVSNRAQ
                     ERMIRKMAERRPGMAKMVKVPGAKHELFGTRGRMLQTYWNMVFAFLEKDL"
     gene            complement(2830..3525)
                     /locus_tag="PLN77_08385"
     CDS             complement(2830..3525)
                     /locus_tag="PLN77_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HRM44323.1"
                     /translation="MIKVILWDIDGTLLNFKMSERYAIEYCFNKFHMGTCTDEMLQRY
                     SVINRGYWERLETGELTREQVMLGRFHEFFESEGLPTDQVKVFNDEYQIRLGDKAFFC
                     DHGDELVKQLKATVKQYAVTNGTTVAQERKLRFSGLDQLLDGVFISEQVGVDKPQKAF
                     FDAVWNEIGSYAPDEVVIVGDSLTSDIRGGKNAGILTCWYNPDHLPNKIEVTPDCTID
                     NLQDILSYLDESR"
     gene            3694..3972
                     /locus_tag="PLN77_08390"
     CDS             3694..3972
                     /locus_tag="PLN77_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRM44324.1"
                     /translation="MKFTYPAVFHKTEQGTYEGYFPDLACCYAKGDTLDEALEDAIHS
                     AYDWISLELTEEEPDFPPVSDVADLGKSEGEIARNIAVNIRLFEGWDE"
     gene            complement(4189..4707)
                     /locus_tag="PLN77_08395"
                     /pseudo
     CDS             complement(4189..4707)
                     /locus_tag="PLN77_08395"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
BASE COUNT         1194 a         1278 c         1107 g         1431 t
ORIGIN      
        1 agcattggaa tgattggaat cattacactt attgaaacgt cacctgatcg attctccctt
       61 ccacccgcat ctcctccccg gaatacgaaa caatccggat tccctctccc acaaggagca
      121 gcctgccata ttgtgtaagc acacagaggc ggcacgcatt atattccaga atcctgcggt
      181 aattttcaat ctgcacttct ctcccgccgt aaaacgtcac aaccggctca ccaaaagcaa
      241 gatctgacgg aacctgcatc gccgaaagca gctccccgcc cggcggatgg agtgattttt
      301 tctgcttttt cttttttcgc atccaaaacc tcctccgctt tgcgggtctg ctttttgttc
      361 tatcgctatt ttatgcagaa aaaagcacaa aaataccccg cagccgctgg ggtttcctgc
      421 ccgatcaggc tgcggggttt catttctctt ttttaaaaac attttttgca gggttcatac
      481 ccctgggctt ctgcattttc tctcgtcatc tgatatgccc gatccggatt cattctgccg
      541 cagttatttt tgttatggta cttcgatccg gtctctgaaa tccacacgag ctgtcctcca
      601 tacggatccg attcctgcgg tgcttccgtt acctgcggtt cttcggcagt cactgccggc
      661 tgtgattctt ccgctgcctg cgtttcaacg gtttcttccg ccgcagcttc tatggttggc
      721 tcaggtgtcg gcgtctcggt cgggtcagcc gttggagttg gggtagcagt cggagttggg
      781 gtagcagtcg gagtcggggt agcggtcggc gttggtgttt cggtcggtgt tgctgtcggc
      841 gttggtgttg atgtttcagt cggggtcggc gtcgcttttt tcgtcggcgc tgccgtaacg
      901 ctgacggaaa cagactctgc agatgccttc tgtgtttcct tactgctgta cgtcacaggt
      961 gtcagcgcac aggcggaaac cagcagcagc acaggaagca gcacaggaag tgctttcgcc
     1021 acatgaaacc gtttctttat cgtattccag gtaaccggaa gcaaaaagat tccaagcagc
     1081 agcagtgaca atgcatatgc cttctcctct ttccagtaac cggctcccgc cagcatcatc
     1141 agaataccga acagatacct gagaaaattt cctgcctttc tcagcatatt tcattccccc
     1201 tcttgtgtat tttctgaatt tcttacagcg aaatcctcta ctgtaaatac gtttgaaaga
     1261 atggaaaatt acatcacaga ttacgattct ttatagaaaa tcatgctgaa ccatatgacc
     1321 atatgctcgc cgaggtaata atccatcatg tttttttcaa tcaggtttgc aacaacgatg
     1381 ccgtggctct cgatgcatgg atgcatcagt tcaagatggc ggcagccgcc tctcgggtag
     1441 gtacatttat cgcagagctg gcaggaatcg gaggaaaggg cgaaggtgag cagggttttg
     1501 tcttttaaat gctgctcgat ttcgcgtgtg attttttcgt gctccgattt tgtttttaat
     1561 gtctcatcca tatccatgat gttgtccact tctgcgatgc tggagaagaa cagaacgtga
     1621 tcgtagttca tgcaacgctc cttacagact tcaacagtgc cgaccgccgg cgggcaggac
     1681 caggaggctc cgtagcggga gcactccttt ttgcagatct ggcggacccg gtcattgaat
     1741 tcgatgtcct ccggttttac aaatgcatac tgatagatag gaaactgggt aatatattct
     1801 tcgatttctg tctgtgtcat tttgtcatcc cttctctttt gttacagatc tttttccaga
     1861 aaagcaaata ccatattcca gtacgtctgg agcatgcgcc ctctggttcc aaacaattca
     1921 tgctttgcgc ccggtacttt taccattttt gccatgccgg gacggcgttc tgccattttc
     1981 cggatcatcc gctcctgggc acggttggac acaagatgat cgttctccgc ctgaaataaa
     2041 aggacaggag cctcgatccg tttccatccg ctccgcatca gatcgcggta cagatgtgcg
     2101 gcggcatgga gccatccgta agaagcggca tttaggtgat acagcggctc tttttcccgc
     2161 ttctgctggt aatagagaaa gcgcggacgg gagagggacg aactttgttt aaagctttcg
     2221 attccctggt atggtttctg tcctaccaca tactgtgtgc ctcgttctgt ggcacaggca
     2281 aaagctgcga tccgttttgc atcggcgaac ggcacttttc cggtcagcgg acggatcatc
     2341 ggcgaactca agaccacttt ccggaacaga tgcggcgctt tggcggccgc tgctgctgcg
     2401 attccgccgc ccatcgagtg agaatagaga acgagcggaa gaccgcatgc ctctgctttt
     2461 gcttttcgtg caacaaagag cagatcttct acataacgat ggtaggaatc gacataaaca
     2521 agcgacggat cctccaccag acggtagctc tttccgtggc cgcagtgttc cggcagataa
     2581 acatgaaaat gctctttgat aaaataatac acgatctctt tatatttctc tgcattttcc
     2641 gtaaaaccgt gtgagattac aacaacacct ctcgggcgga atgcgcggta acgaagaaca
     2701 tgaatcggat gaccgctctc cctttcccag taaaattctt ctgcatgagc ggcgaggtac
     2761 ggctccacac cattctgcat ccgctccgca taattttttt cctgtaatac gcgtgttctc
     2821 attagcaaat cacctgcttt catccagata cgaaaggata tcctgaagat tgtcgatggt
     2881 acagtccggt gtaacttcaa ttttgttcgg aagatgatcc gggttgtacc agcaggtcag
     2941 gattcctgca ttttttccgc cgcggatatc acttgtgagg gagtcgccga cgatcacaac
     3001 ctcatccggt gcataacttc cgatttcgtt ccataccgca tcaaaaaaag ccttctgcgg
     3061 cttgtccaca ccgacctgtt ccgagataaa aacgccgtcc agaagctgat caagcccgga
     3121 aaagcggagt ttacgctcct gtgcaacagt ggttccattt gttactgcat actgttttac
     3181 ggtagctttc agctgtttta ccagttcatc tccgtgatcg cagaagaaag ccttatcgcc
     3241 gaggcggatc tgatattcat cattgaatac ctttacctga tccgtcggca gtccttccga
     3301 ttcaaaaaat tcatggaacc gtccaagcat cacctgttct ctcgtaagct ctcccgtctc
     3361 cagccgctcc cagtaaccgc ggttgatcac gctgtagcgc tgcagcattt cgtccgtgca
     3421 ggttcccata tgaaacttgt tgaaacagta ctcaattgca tatcgttccg acatcttaaa
     3481 attcagaagt gttccgtcga tgtcccacaa aattactttt atcaaagaga aatcctccct
     3541 tggtttcatt tgtattttcc aattatctgt ttttcattct agcatagatt acaaatacga
     3601 tcaattgtat tttaggcatt gtatcttttg agaaaaagtg ctacgataaa aaagaatttc
     3661 cgtgaaaaag aaaaggaaga aggaagaaac actatgaaat ttacttatcc agccgttttt
     3721 cataaaaccg aacagggaac ctacgaaggt tattttccag atctcgcctg ctgctatgca
     3781 aaaggggata ccctcgacga ggcgctggaa gatgccattc actccgccta tgactggatc
     3841 tctctggaac tgacggaaga agaacctgat tttccgccgg tatcggatgt ggcagatctc
     3901 gggaaaagcg aaggtgaaat tgcaagaaat attgccgtca acatccggct ttttgaggga
     3961 tgggacgaat aagttcaaaa aagagaaaga aaaccgtatt gctgcgatat tcgaaacgcg
     4021 gcgatacggt tttcttttta tctcttccag gttttcagca tctctttttc ttctttgctg
     4081 atccgcagcg actctctgat tttctgaatg tgtccagtca tcgagactgt tttctttcag
     4141 cagtttctgt gtcttttccg gaaaatgaat ataacagatc gatactgccc aggcgacggc
     4201 catttttacg taatagccat cgtgatgaat ctttttcatc tcctccagca cagcatcgat
     4261 atacgcttcg ttgataaaaa agtccagcag acacacaaca ccaaaccgga tttcatattc
     4321 ccggctgctt ttcagccatt tctgcagata tccataccaa tactcctggt ctttcttcat
     4381 aaaatggcac gttgcacagg agctgtcaca gattccccag ctgttgatca tcggcacgaa
     4441 gcggtccagt tctgccgtct gttcttcacg ggtcagcttt ccgtagccga tcatcattcc
     4501 gcggatcaga agctcctcgt agacttccgg attaaactgt tcggtataaa cttcatagtg
     4561 gtctcttgct gtcgcttttg caatccgtcg cgaaaccgga atgcggacac caagaatccg
     4621 acagttctgt cccggaacaa gagagctgtg aaattcccgg tattttgcat ctatattgct
     4681 ttccagttct gcccgaatgg cctgcattac ttcctgttcc atatgattcc ccctctcttt
     4741 tctttctctt taagatgttt tacacgcttt ttacaaatgt ttaaccctgt tatttctgca
     4801 cttctgataa gataaaatta aagtaaacga taacatcccc cctgtcaaat gccagatctt
     4861 ttccggctga aaggtgagtc ttatgaaaaa gaaatataca ttttttcaaa atataccatt
     4921 cctgtgcaaa tttcagaagc agccagccct tgcagaagaa gcgccagctg tttccacaac
     4981 gctgtctatt ccgttttccg gacctgcaga
//
LOCUS       DAOMUF010000151         4976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4703_length_4976_cov_2.391587, whole genome shotgun sequence.
ACCESSION   DAOMUF010000151 DAOMUF010000000
VERSION     DAOMUF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4703_length_4976_cov_2.391587"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..859
                     /locus_tag="PLN77_08400"
     CDS             386..859
                     /locus_tag="PLN77_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44325.1"
                     /translation="MSSKTKIIVLHMKEVVYTTVFLALAILLLVVFLIMFGSGKKESA
                     KNPAASSTGSAESARYIPGIYTSTISLGDQTFDIQVDVEQDRITAISMNNLSETTAAM
                     YPLMEPALDSIASQIYVNQTTEGLIYGEDNRYTSELLVMAIDQALDQARNPSATK"
     gene            complement(1048..2956)
                     /locus_tag="PLN77_08405"
                     /pseudo
     CDS             complement(1048..2956)
                     /locus_tag="PLN77_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950316.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
     gene            complement(3046..3921)
                     /gene="hslO"
                     /locus_tag="PLN77_08410"
     CDS             complement(3046..3921)
                     /gene="hslO"
                     /locus_tag="PLN77_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRM44326.1"
                     /translation="MDYIVRATAADGQIRAFAATTRETVETARQDHNTSPVATAALGR
                     LLTAGAMMGVMMKGDKDLLTLRIHAGGPLNGITVTADSKGNVKGYVGNPDVVIPANKK
                     GKLDVAGAVGVGFMDVIKDLGLKEPYVGQCVLQTSEIAEDLTYYFATSEQVPSSVGLG
                     VLMNKDNTVRQAGGFIIQLMPFCDEKVIEKLEQRLAEVNSVTAFLDEGLTPEQILEKL
                     LGDMDLVINDTVPTQFYCNCSRERVSKALIAVGRKELNEMIQEAKPVELKCHFCNSAY
                     EFSTEDLKELLRKSK"
     gene            complement(3931..4716)
                     /locus_tag="PLN77_08415"
     CDS             complement(3931..4716)
                     /locus_tag="PLN77_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRM44327.1"
                     /translation="MKETKRMNESYTSFAQVYDMFMDNVDYPSWSKYLIQLLKEYQVE
                     DGLVLDLGCGTGNMTELLAKEGYDMIGVDNSEDMLEIASEKRAESGLNILYLLQDMRE
                     FELYGTVKAVVSICDSINYILEEDDLREVFSLVNNYLDPKGMFIFDLNTKYKYEQMGE
                     TTIAENREEASFIWDNYYDPEEEINEYELAIFIPEGEDSDLYRKFEEVHYQRAYDLAT
                     IRRLLEEAGMEFVTAYDAFTKDAPRPESERIYVVAREKGKSEK"
     gene            complement(4875..4946)
                     /locus_tag="PLN77_08420"
     tRNA            complement(4875..4946)
                     /locus_tag="PLN77_08420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4912..4914),aa:Asn,seq:gtt)
BASE COUNT         1190 a         1386 c         1024 g         1376 t
ORIGIN      
        1 taaatctgca gtgtattttc caccaggttt tcctcgtttt ttttagcaat atcaacaata
       61 aacgctttgt acagaaacag taaaataatc tgcagaagca aaaaagtagt aaaaaaaacg
      121 cccatggtcc ttctgatttt tctttttttc atctttcccc tcttcttttc ccaactcttc
      181 tctgaaatct gttatcgtga ccgattacat tatatactaa ttctcgggca gaagaaaggc
      241 tgtctaaaaa ttatatctgg aatttgcatg ttacgaaact ttctttcata aacgattttt
      301 cttttacata catcatagta aaaacgcccg tccgttcgcc ctcgtccgct cacagacggt
      361 ttttcaaaaa ggagtgtgcc cgcccatgag ttccaaaacg aaaatcattg tactacatat
      421 gaaagaagtg gtttacacca ctgtttttct cgctctcgcg atccttctgc tcgtggtttt
      481 tctgattatg ttcggttccg gaaaaaagga gtctgcgaag aaccctgcgg cctcatccac
      541 cggttctgcc gagagtgcac gctacattcc cggcatctat acctccacaa tttctctcgg
      601 tgaccagacc ttcgatatac aggtcgatgt cgagcaggac cgcattaccg caatctcaat
      661 gaataaccta agcgagacca cggccgctat gtacccgctt atggagcctg cactcgattc
      721 cattgcaagt cagatctacg taaaccagac aaccgaaggc ctcatttacg gcgaggataa
      781 ccgctacacc tccgaactgc ttgtcatggc aatcgaccag gctctcgatc aggcccgcaa
      841 tccttctgca accaaataaa gttaaaaatg gctcctgagt gttctcactc cgaagccatt
      901 tttctgttgt ttttctattg cttttctgtt gtcttatctg tcaatccttg attctgtgaa
      961 ccagacacac tctaatgctt atcgtccgaa cagtttaccg cgtcaatcgc aagcaccaga
     1021 agaagcgccg gaatctcatg cgatggatta cagccggtca agctgttctc tccagagttt
     1081 cacgcttgca tcacttggca tgcgcagatc gcctcgcggg gaaaccgcaa cgcttccgac
     1141 cttcggtccg tccggcaggc aggaacgctt aaactgctgt gcaaaaaatc tccagtagaa
     1201 ggttttcagc cacttcagga ttgttgtatc atcgtaaaca ccggcaaacg caagtttcgc
     1261 cacacggtaa attttcagcg gctcgtacgt cacacgcagc atatagtaca aaaagaaatc
     1321 gtggagttcg tacggtccga caagatcctc tgtcttctgg gaaatgacac cgtctttcgg
     1381 cggaagcagc tccgggctga ccggcgtatc aagcacatcc aggagaatat cagaaagtgc
     1441 tttctctccg caggtatccg cataatactg caccagatgg cgcaccagcg ttttcggtac
     1501 agaagcattg acggcataca tcgacatatg atcgccgttg tacgttgccc acccaagtgc
     1561 cagttcggaa agatctcccg tcccaatgac catgccgccg gactggttgg ccagatccat
     1621 cagaatctgc gttctctcac gcgcctgtcc gttttcatac gtcacatcgt gtttctccgg
     1681 attctgtccg atatcacgga aatgaagatt gacagcctcc ttgatatcga tctcttttaa
     1741 ggttgcgcca aggcatctcg tcagctcaca tgcattctga tacgtacggt ctgtcgttcc
     1801 aaaacacggc atcgttacag acaggatctg ctttctcgga atcccgagca gatcaaatgc
     1861 tcttgccgtt acaagcaatg ccagcgtcga gtcaagtcca ccggaaatac cgataactgc
     1921 gctctggcaa tgggtatgcg ccagacgttt tttcagtccc atagcctgaa tatttaaaat
     1981 ttcgtcgcat cgctgatcgc ggttatgctt atccgacggt acgaatggcc gtggatcgaa
     2041 gtaacgggtc agctttgtct cttctattga caaggagaat cgaacgacct gatacccatc
     2101 ggttctctgc tccggatacg tggtcatcct tctgcgctca cttgtgagac gatccacatc
     2161 gagatctgca caaatgatct gattctcaaa tcgttttgcc tcggcaagaa tggttccgtt
     2221 ttcagcaatc agatgctgtc cgccaaagac aagatctgtc gtcgactcgc cctcgcctgc
     2281 tgtcgcgtat atatagccgc agatcagacg cgccgactgt gcgctcacaa ggcttctccg
     2341 gtaactgtct tttccaacca tttcatcact ggcagaaagg tttacgacca ctgtcgcacc
     2401 tgcaagcgca tgcgccacac tcggcggatt tggggtccag agatcctcac agagctctgc
     2461 cgcaacctta agacgcggca ttttttcaca ctggaagagc agattcatgc caaacggaac
     2521 ctcttttcca aaaaattccg ttgttgtcac cgcttctttt cccggcgtaa aatgacgcag
     2581 ctcataaaat tcattatagt tcggcagata ctttttcggt acaacaccga gaagctccgt
     2641 gacaaagcac agcggctaca ttgaacagct ttccaccctg ttcccacggc agtccgacaa
     2701 aaatcagcgc atcggtctct ttcgtgccat cggcaatttc ctttaatcct tctttcgccg
     2761 catcaatcat ctgactctgc cagaataagt cactacaggt gtagccggtg agagccagct
     2821 ccgggagcac catcaccttt gctccctcgc cctccatttt atgaatcgtt tccagaatga
     2881 ctctcgtatt ttctctgaca tccgctacca ctaccgcaca ggaagcggcg gcaactttta
     2941 cgaatccctg tttcatctct ttcttcctcc actatctggt tccctgttac agattttctg
     3001 caaacagaag ctctcacagc ctccgtttcg cctgaaacag tactgttatt tgctcttacg
     3061 caataattct ttcagatcct cggtagaaaa ttcataggcg ctgttgcaga aatggcattt
     3121 cagctccacc ggttttgctt cctgaatcat ctcgttcagt tcttttcttc cgaccgcaat
     3181 cagcgctttc gatacacgct cacggctgca gttgcagtaa aactgggtcg gcacggtatc
     3241 gttgatcaca aggtccatgt ctccaagcag tttttcaaga atctgctccg gagtcaggcc
     3301 ctcgtcaagg aatgcggtaa cggaattgac ttctgccaga cgctgctcca gtttttcaat
     3361 gactttctca tcgcagaacg gcatcagctg aatgataaag ccgccggcct gacggacggt
     3421 attgtctttg ttcatcagca caccgagtcc gacgctggac ggaacctgct cactcgttgc
     3481 aaaatagtag gtcagatcct ctgcgatctc actcgtctgc agaacgcact gtccgacgta
     3541 cggctctttc agtcccaggt ctttgatgac atccatgaat ccgacgccga cagcacctgc
     3601 cacgtcaagt tttccttttt tgttggccgg aatcacgacg tccgggtttc cgacgtaacc
     3661 ttttacattt cctttggaat ctgctgttac ggtaatgccg ttcagcggtc cgccggcatg
     3721 gatgcgcagg gtcagaaggt ctttatctcc cttcatcata acgcccatca ttgcacctgc
     3781 cgtcagaagg cggcccagcg ctgcggttgc aaccgggctc gtgttgtgat cctggcgtgc
     3841 tgtctctacg gtttctcttg ttgtcgctgc aaacgcacga atctgtccgt ctgccgctgt
     3901 tgctcttaca atataatcca tgtttctctc ctatttctca ctttttcctt tttctcttgc
     3961 tacgacataa atacgttcgc tctccggtct cggcgcatcc tttgtgaatg catcgtaggc
     4021 cgtcacaaac tccatgcctg cctcttccaa taagcggcgg atcgtcgcaa gatcgtacgc
     4081 tctctgataa tgtacctcct caaatttacg gtacagatcg gaatcttctc cctccgggat
     4141 gaaaatggca agctcatatt cgttgatctc ttcctccggg tcatagtaat tatcccagat
     4201 gaagcttgcc tcttcgcggt tttccgcaat cgtagtctca cccatctgtt catatttata
     4261 cttggtattc agatcaaaga taaacatgcc cttcggatcc agataattgt tgacgagtga
     4321 gaagacttcc cgcagatcgt cttcctccag aatatagttg atagaatcgc agatgctcac
     4381 gaccgctttc acggttccgt aaagctcgaa ttcccgcata tcctgaagca gatataaaat
     4441 gtttaagccg gactcggcgc gtttttcact ggcaatctcg agcatatcct cagaattgtc
     4501 cacgccgatc atatcgtagc cctctttggc gagcagctcg gtcatgtttc ccgttccgca
     4561 gccaagatcg agcaccagtc cgtcttccac ctgatattct tttaaaagct gaatcagata
     4621 tttgctccag gacgggtaat ccacgttgtc catgaacatg tcgtaaacct gagcaaaact
     4681 ggtataactt tcattcatcc tctttgtctc cttcatttgt ttcggatagc cctattgtaa
     4741 cacatcagaa agggcaactc aattctcttt tgtctctgtc acccctagga tgacacatct
     4801 tacagtaaac cctttttcat cacgcctgca agaaacgaaa agacctgcaa gaaaattctt
     4861 acaggtctca ttcactcccc gagttgggct cgaaccaaca acccctcggt taacagccga
     4921 gtgctctacc attgagctat cgaggattat tcaagaaagt acaaaagaga agcttt
//
LOCUS       DAOMUF010000152         4928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4766_length_4928_cov_3.420275, whole genome shotgun sequence.
ACCESSION   DAOMUF010000152 DAOMUF010000000
VERSION     DAOMUF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4766_length_4928_cov_3.420275"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="PLN77_08425"
     CDS             <1..1759
                     /locus_tag="PLN77_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM44328.1"
                     /translation="TIDVTKESFASYDDSGATGHKSCYVLEEGSYEFYVGSDVRSAAF
                     AGAYEQPFKVVEILTEAMAPVEAFERMKAVPGEDGTLKPGYEAAPLRTVDPIERMKEN
                     RMEPITYTGDKGYKLGDVLDKKVTMEEFVAQLSDEDLICIFRGEGMCSPKVTPGTAAA
                     FGGLTPELQEFGIPAACCTDGPSGLRFDCGTRAFSMPNGTLLGCTFDLPLVEDLYEMA
                     GREMRQNRVDALLGPGMNIHRNPLNGRNFEYISEDPYLTGWISAVQILGMEKSDVTGT
                     IKHFCANNQESNRHHVDAVVSERALREIYLKGYEIAVKEGGARSIMSTYGPVNGIWTA
                     GNYDLLTTILRGEWNYDGFVMTDWWAMSNREGYEATKTTHAPMVSAGNDVFMVCTDCS
                     DMGQDDVKEALENGEITRGDLQRNAMNVLHFILGTPSILRFLDRISEEEKEAQEQMGD
                     NDFVAADLVTYEADPATGDVVIDASAWNTKKGNSEVCGVTILADKMGTYDIEIEMKSD
                     LEDLAQLPVTVYIDNIVKTMISIRGTKGEWIKETRDLGFFFGPNHYLKLYFGANGLEL
                     GKIRLKLREGMEVLSKHEE"
     gene            complement(1870..2499)
                     /locus_tag="PLN77_08430"
     CDS             complement(1870..2499)
                     /locus_tag="PLN77_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRM44329.1"
                     /translation="MKQFFQDVKNSFLLSAAVSIILGIVLIVYPVKTSLLICYIAGAL
                     LLFGGVVALIRYIASRGEPFFFRYDLFVALVLILFGCFVIFESDLVIAFIPVVIGIIL
                     LANGLLSIQKAFNLKKTGLEKWWLEFLLALLTSVLGIIICTNPFDAVATTNIFIGICL
                     VYSGISDLVTTYYMGRAKRRIKNVIQTEINQQKILHDDNVIDVDEDELH"
     gene            2640..3386
                     /locus_tag="PLN77_08435"
     CDS             2640..3386
                     /locus_tag="PLN77_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HRM44330.1"
                     /translation="MQDEQKKFQEKLSELLSYARNHENKVTMKEVRDFFEDFALDEQK
                     VTFVCEYLTMEQVDVADYEPGVVPEETEKEKKKPEFSEEELRALQQYLDELPETETPS
                     EEETAELYRKAAEGDSLAKSMLVQLWLPKVIETAKEMHTRDFFLMDLVQEGNVGLLVA
                     LESVVKAETAEQAIDAAVRETISDFMEEHRVQKHKDNTVVNKVNRLKDAIEELSDGDD
                     MDFSVAELSAYLDMSVEEIEDILRIAGEEP"
     gene            3456..3758
                     /locus_tag="PLN77_08440"
     CDS             3456..3758
                     /locus_tag="PLN77_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HRM44331.1"
                     /translation="MVHHIVMWKFKPEVEDAKKPELKKAMAENLKSLVGKVPGLLTVD
                     FVEAPLASSTHDMALVTTLEKAEDVAVYAKHPEHVKVADTYVRPYTTERAALDYEE"
     gene            complement(4044..>4928)
                     /locus_tag="PLN77_08445"
     CDS             complement(4044..>4928)
                     /locus_tag="PLN77_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44332.1"
                     /translation="TYSLSQKTLLADDTVDFGFSDDITINEPEGLCETNPGEFIAIVN
                     VTKADGGRYVQFYRTRVPYLFTVSTSAAEHGSVSEGGEVSRGEEKTVSYTADEGFRLA
                     KLSVDGLYLPVTEYPDSYTFSDIQKDHTLAVTFEPVPTATPTPTATATPTETPAITQT
                     PEPAVSKAPASSTVSFSSVASHFAAFWKGTVHAASWFRHLASAGASLFARIRKADPVF
                     LAMPFVLLVLILLFVLRLRYVRRIRRERSRKALEHRRRTYEELVREFTEDPKDEKVSI
                     DELLLTIDELQKEEQKNT"
BASE COUNT         1396 a         1138 c         1342 g         1052 t
ORIGIN      
        1 gacgatcgat gtgaccaaag agagctttgc atcctacgat gacagcggtg caaccggcca
       61 caaatcctgc tacgtactcg aagagggcag ttatgagttc tacgtcggaa gcgatgtcag
      121 aagcgcagcc tttgccggtg catacgagca gccgttcaaa gtggttgaga tcctgaccga
      181 ggccatggct ccggtagaag catttgagcg catgaaagct gttccgggcg aagacggaac
      241 gttaaaaccg ggatatgagg cagctcctct gcgcaccgta gatccgatcg agcgcatgaa
      301 agaaaaccgc atggagccga tcacctatac cggtgacaaa ggctacaagc tcggtgatgt
      361 cctggataag aaagtaacga tggaagagtt cgtggcacag ctttccgatg aagatctgat
      421 ctgcatcttc cgcggcgagg gcatgtgcag cccgaaggtt actccgggaa ccgcagcagc
      481 attcggcggc ctgacaccgg aactgcagga attcggtatt ccggcagcct gctgtaccga
      541 cggcccgtcc ggactccgct ttgactgcgg aacgagagcc ttctccatgc cgaacggaac
      601 cctgcttggc tgtacgtttg accttccgct ggtagaagat ctgtacgaga tggcaggacg
      661 cgagatgaga cagaaccgtg tcgatgccct gctcggacct ggtatgaata ttcacagaaa
      721 cccgctgaac ggccgtaact ttgagtatat ctccgaggat ccgtacctga ccggatggat
      781 cagtgccgta cagattcttg gtatggaaaa atccgatgta accggaacca tcaaacactt
      841 ctgtgcaaat aaccaggagt ccaaccgaca tcatgtggac gccgttgttt ccgagcgtgc
      901 cctgcgtgaa atctatctga agggatatga aatcgcagtc aaagaaggcg gcgcaagatc
      961 catcatgagc acttacggac cggtcaacgg tatctggact gcaggaaatt acgatcttct
     1021 gaccacgatt ctgcgcggcg agtggaacta cgacggcttc gttatgactg actggtgggc
     1081 aatgtcgaac cgtgagggat atgaagcaac caaaacaacc catgcaccga tggtatctgc
     1141 cggaaacgat gtcttcatgg tatgcaccga ctgctccgat atgggacagg acgatgtgaa
     1201 agaggcactg gaaaacggcg agatcacaag aggcgacctg cagagaaatg cgatgaatgt
     1261 cctgcatttc atccttggaa ctccaagcat cttacggttc ctggaccgca tcagcgagga
     1321 agagaaagaa gcacaggagc agatgggcga caacgatttc gttgcagcag atcttgtaac
     1381 atacgaggca gatccggcaa cgggagatgt tgtgatcgac gcatccgcat ggaacacgaa
     1441 aaaaggaaac agtgaagtat gcggtgttac gattctggct gacaagatgg gaacgtacga
     1501 tatcgaaatc gagatgaaat ccgatctgga agacctcgca cagctgccgg taaccgtgta
     1561 catcgacaat atcgtaaaaa ctatgatcag catccgcgga acaaaaggtg agtggatcaa
     1621 agagacgaga gaccttggct tcttcttcgg cccgaaccac tacctgaagc tgtacttcgg
     1681 cgcaaatggt ctggagcttg gaaagatccg cctgaagtta agagaaggca tggaagtact
     1741 gagcaaacac gaggaataaa taaaaaaaga aatacagaga aaaaccggaa cccgggagcc
     1801 gctgtttgaa agcggtgccg ggttccggtt tttcgtatga aagaagattt ttatggaaga
     1861 agctgttttt tagtgaagct cgtcctcatc cacatcaatc acattgtcat cgtgcaggat
     1921 tttctgctgg ttgatctcgg tctggatgac gtttttgatc cggcgttttg cgcggcccat
     1981 gtagtaggtg gtgacaagat cggaaatgcc gctgtagaca aggcagatac cgatgaaaat
     2041 gtttgtggtt gcaacggcat caaacggatt ggtacagatg atgatgccga gaacgctggt
     2101 taaaagagcc agcaggaact caagccacca tttttccagc cctgtttttt tgaggttgaa
     2161 cgccttctga atgctgagca gtccgtttgc cagaaggatg ataccgatga caaccgggat
     2221 gaatgcgatt acaaggtcac tttcaaagat aacgaagcag ccgaacagaa tcagtaccag
     2281 ggcaacgaac agatcgtagc ggaagaaaaa cggctcgccg cggcttgcaa tgtagcggat
     2341 caacgcaaca acgccgccaa acaggagcag tgctccggcg atataacaga tcagcaaact
     2401 tgtttttacg gggtagacga tcagcacgat acctagtata atagagacag cggctgaaag
     2461 caggaaactg tttttcacat cctgaaaaaa ttgtttcata aggattctcc tttccacttg
     2521 aatgtggttt ttcgtttgct tttatagtag cttttcaaag cagaaaaagc aatggacaaa
     2581 ttgaaaaaaa tgtacgaaga aatggaatcg tgaaacggaa gaaagagagg gtaaaaaaga
     2641 tgcaggacga acagaaaaag ttccaggaga agctctccga attgctgtcc tacgccagaa
     2701 accatgaaaa taaagtaacg atgaaagaag tcagagactt ttttgaagac tttgcactgg
     2761 atgagcagaa agttaccttt gtctgcgaat atctgacgat ggagcaggtc gatgtggcag
     2821 attatgagcc gggcgtcgtg ccggaggaga ccgaaaaaga aaagaaaaaa ccggagtttt
     2881 ccgaggagga gctgcgtgcc ttacagcagt atcttgacga actgccggag acagaaacac
     2941 cgtcggagga agaaacggca gagctttacc ggaaagcggc agagggcgat tcccttgcaa
     3001 agagcatgct cgtgcagctc tggcttccga aagttattga gaccgcaaaa gaaatgcaca
     3061 cccgcgattt cttcctaatg gatctggtgc aggagggaaa tgtcggactt ttagtagcgc
     3121 tggagtccgt tgtaaaagct gagacggcgg agcaggcgat tgatgcggct gtgcgtgaga
     3181 cgatcagcga tttcatggaa gaacacagag ttcagaaaca caaggacaat actgtagtca
     3241 ataaagtaaa ccgcttaaaa gacgccatcg aggaactctc cgacggcgat gacatggatt
     3301 tcagtgtggc agagctttcc gcgtatcttg acatgtccgt ggaagagatt gaggatattc
     3361 tgcggattgc aggcgaagag ccgtaacaaa aggctggtat ttgcacagaa actgtgtgaa
     3421 ttggcaataa aacaaaagca aaggagaaaa atacaatggt acatcatatt gttatgtgga
     3481 aattcaaacc ggaagtagag gatgcaaaga aaccggagct gaaaaaagcg atggcagaga
     3541 acctgaaaag cctggtcggc aaagtgccgg gactgttgac ggttgacttt gtagaagcac
     3601 cgcttgcatc ttccacccat gatatggcac ttgtcacaac actggaaaaa gcagaggatg
     3661 tagctgtata tgcaaaacat ccggagcacg taaaagtagc agacacctat gtacgtccgt
     3721 acacaacgga gcgtgcagca ctcgattacg aagagtaaaa acaaatacgg actttagagc
     3781 gtgtctgaaa aaccatttcc gcaatctgca agccccactt tgcgggagat tttaccctca
     3841 ttcggttgcc gtagcccgct acgccgccct cattcggata aaatctccca caaactggga
     3901 ctcgcagctc acgaaaagcc tttttcagac acgctctagc tcgttgcccg cgtaaataaa
     3961 aaagtatccc gcttccccaa aaggaacggg atactttttt tgtgccgaga agatcaattc
     4021 ctgatctggt gaatacgttt catttaagtg tttttctgtt cttccttctg cagttcgtca
     4081 attgtaagca gcagctcatc gatggaaacc ttttcgtcct tcggatcttc cgtaaattct
     4141 ctgaccagtt cttcgtacgt tcttcttctg tgttccagcg ctttccgcga ccgctcccgc
     4201 cggatgcggc ggacataccg caggcggagg acaaagagca gaatcagaac gagaaggaca
     4261 aacggcatcg ccagaaaaac aggatctgcc ttccggatac gggcaaaaag ggatgcaccg
     4321 gccgaggcaa gatggcggaa ccaggaagcg gcatgcacag tgcctttcca gaacgctgcg
     4381 aaatgggaag ccacagatga gaaggaaacc gtggatgatg ccggtgcttt ggaaacggcc
     4441 ggttccggtg tctgggttat ggccggtgtt tcggtcggcg tcgcagtggc agtcggcgtc
     4501 ggtgtcgccg tcggaaccgg ttcaaacgtt acggcaaggg tatgatcctt ctggatatcg
     4561 gaaaatgtgt agctgtccgg atattccgtc accggcagat acaagccgtc caccgagagt
     4621 ttcgcgagcc ggaagccttc gtctgccgtg taggaaacgg ttttttcctc tccgcgggaa
     4681 acttccccgc cctcggacac cgagccatgc tccgccgcgg aagtggaaac cgtgaacaga
     4741 tacggcaccc gggtgcggta gaactgcacg taccgtccac catctgcctt tgttacgttt
     4801 acaatcgcaa taaattcccc aggatttgtc tcgcagaggc cctctggttc attgatggtg
     4861 atatcgtcgg aaaagccaaa atcaaccgtg tcgtccgcaa gcagtgtttt ctgcgagagc
     4921 gagtatgt
//
LOCUS       DAOMUF010000153         4910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4786_length_4910_cov_2.658908, whole genome shotgun sequence.
ACCESSION   DAOMUF010000153 DAOMUF010000000
VERSION     DAOMUF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4786_length_4910_cov_2.658908"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLN77_08450"
     CDS             complement(<1..289)
                     /locus_tag="PLN77_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRM44333.1"
                     /translation="MGTEFFWFYDVILAAILIGMMFIGAKKGFVRMVLRLCAFAASFV
                     IALMISNGVSGWIYDSFISKPLEQTISTSINDALGDNVVTQLGKIDMEKAKI"
     gene            complement(392..1030)
                     /locus_tag="PLN77_08455"
                     /pseudo
     CDS             complement(392..1030)
                     /locus_tag="PLN77_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
     gene            complement(1242..2981)
                     /locus_tag="PLN77_08460"
     CDS             complement(1242..2981)
                     /locus_tag="PLN77_08460"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM44334.1"
                     /translation="MKKQDKRNWPKAVIQILSFLLIPGLFEAQFSAVGTIISAVYQGT
                     VSWERIRYSVWMLLATVPAVIIVGRFFCGYFCSFGAVQDFLWFAGNKLKKRFRKAGAK
                     NLHSNSKKSGRNVPTGWIKYGVLLFWAVFVWSGMIKWNIAGPWQVFGQYSSIGHWPGV
                     KPMFSIGGALILVIFVGSVLIQRFFCRYLCPMGAIYSLISRCALLKIVKPRSKCGNCR
                     LCTAKCTMGIDLAEKKQTAGGACIGCGVCVNGCPKNNAHWKYRYSVWIGVGVTCLTIA
                     ASQFLIRSESGSVGKTAGDAAIDTAEQNLDSRFQDGTYSGSGEGYRGTVTVSVKVEQG
                     AITGLTIENAEDDQTYLDRAKSGIFSEIMKKQSAEEIDTVSGATYSSKGLIEATQNAL
                     KEADQEKTDEPDENTASISAAGDENSKNETQENTASESTDTTEEKQAFLDAGRFHDLK
                     DGIYTGTADAFRGDVEVQVTVENQKVTDISILSYCDTEEYFFRAAPYVIEQMKEEQSL
                     NIDALSGATYSSNGIIHATANALEIPEDEYAPRPGRDLKNKQKDHGHIVQHTIENREQ
                     YEEKVKEYEDVQN"
     gene            complement(3236..4174)
                     /gene="tsf"
                     /locus_tag="PLN77_08465"
     CDS             complement(3236..4174)
                     /gene="tsf"
                     /locus_tag="PLN77_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRM44335.1"
                     /translation="MAITAAMVKELREVSGAGMMDCKKALTATEGDMDKAMEFLREKG
                     LATAQKKAGRIAAEGIVMLNVSEDGKKAVAVEVNAETDFVAKNEKFRGYVAQVAEQAM
                     DTTAADVEGFLAEPWKFDTNETVGEELAHQIATIGENMNIRRFQQVKEENGFVASYTH
                     MGGKIGVLVDVETDVVNDDLKEMAKNVAMQIAALRPQYTNRDEVSADYIAHEKEILMA
                     QIQNDPKESQKPEKVIQGMIQGRINKELKEICLLDQVYVKAEDGKQSVAQYVAQVAKA
                     NNATVAVKGFVRFETGEGIEKKEENFAEEVAKQMNA"
     gene            complement(4295..>4910)
                     /gene="rpsB"
                     /locus_tag="PLN77_08470"
     CDS             complement(4295..>4910)
                     /gene="rpsB"
                     /locus_tag="PLN77_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRM44336.1"
                     /translation="IIDLQKSVGMVDDAYKAVADIAADGGTILFVGTKKQAQDAIAVE
                     AERCGMYYVNERWLGGMLTNFKTIQSRIARLKAIETMEQDGTFEVLPKKEVIELKKEL
                     AKLQKNLGGIKEMKRLPDAIFVVDPKKERICVQEAHTLGIPLIGICDTNCDPEELDYV
                     IPGNDDAIRAVKLIVSKMADAVIEAKQGEAEEAAYEEAAETDAE"
BASE COUNT         1082 a         1302 c          969 g         1557 t
ORIGIN      
        1 ttattttcgc cttttccata tctatcttgc ctaactgtgt aacaacgttg tcaccgagcg
       61 catcgtttat agaagtcgag atagtctgtt caagcggttt actgataaag ctgtcatata
      121 tccagcccga aacgccgttg cttatcataa gcgctatcac aaatgacgcc gcaaaggcgc
      181 acagcctcag caccattcgg acaaagccct ttttcgctcc tatgaacatc atacctataa
      241 gtatagccgc aagaataaca tcataaaacc agaaaaattc agttcccata ttctttcctt
      301 tctgcaatta aagctgtatg caatcaaggc tgtcataccc cataagaagt attgagccgc
      361 caacctttat aaaatccgaa tagctgtctt taagcagagt tttctttaca gactgatccg
      421 gtttcaggat aacaaattca ttttcatgaa attccgggct gatctgtttt ccttccgggt
      481 cgctctggta agcatccgcc aaacaaattc cttttttctt aaattccttc accttatctt
      541 ccggaatgta aacttctatc cgcccggtat attgtgcatc gttatcagag atccgctctt
      601 tttcaaaatc ttccgccgga attcccagaa tctgcgcctt cagtcttaat acaatatctt
      661 tgctgaccag tataatctga tcatttttat ctgcatcggc aagtcctttg catactttca
      721 gaattctgtt atctgcctta tcttccggaa gatcctgcgg cagttctacc tgcacaaaat
      781 ttttttcaat ccgcagactg cctccccctg gcagttctac cccctgtaat aaatcgccgc
      841 actgtctgta tctttccagg ttccgaattg cttttcttgc attcgcaccc cgctctcctt
      901 ctgccttttt cagtccatcc agttcttcca gcactaccag cggcagtacc agacggttat
      961 catcgaagca ttccaaggca tacggtgcct gaatcaatac atttgtatct attacatagg
     1021 tttttatcat acactcgagc actccttttt ttctgtcttt tttactacaa tcacgatcgt
     1081 agcacatttg tattttaact gctatcctgt tccgataagg tctatatcaa atgattgtaa
     1141 aattttttat atttctgatg cagtcagacg gggttctgtt acagcagaaa agcactgcaa
     1201 atacagatgg atgtatttac agtgcttatt ctgagatttt tttaattctg tacgtcttcg
     1261 tattctttta ctttttcttc atactgctcc cggttctcga tggtatgctg tacaatgtga
     1321 ccatggtctt tctgtttatt tttcagatca cggccggggc gcggtgcata ttcgtcctcc
     1381 gggatttcga gggcattggc agtggcgtgg atgatgccgt tgctgctgta ggtggcgccg
     1441 ctcaaggcgt cgatgttgag actttgttct tctttcatct gttcaatcac gtacggcgct
     1501 gcacggaaaa agtattcctc ggtgtcgcag taggaaagga tggagatgtc ggtcactttc
     1561 tggttttcta ccgttacctg cacttcgaca tccccgcgga aggcatcggc ggtacccgtg
     1621 tagatgccgt ctttcaggtc gtggaaacgg ccggcgtcca ggaatgcctg tttttcttcc
     1681 gttgtatccg tcgattcaga tgctgtattt tcctgtgttt cattcttgga attctcgtct
     1741 cccgcagctg atatggacgc cgtattttcg tctggttcat ccgttttttc ctggtcggct
     1801 tcttttaacg cattttgagt ggcctcaatc aaccctttac tgctgtaggt ggcgccggaa
     1861 acggtatcta tctcctcggc actctgtttt ttcatgattt ccgaaaagat tcctgacttt
     1921 gctctgtcca gatatgtctg atcatcctcg gcgttttcta tcgtcagtcc ggttatggct
     1981 ccctgttcta cttttacgga aacggtcacg gttccgcggt agccttctcc acttccggaa
     2041 taagtgccgt cctggaatct gctgtccaga ttctgttctg ccgtatcaat ggccgcatca
     2101 cctgctgtct taccaacgga accgctctcg ctccggatca ggaactgtga cgctgcaatc
     2161 gtaaggcagg tcaccccgac accgatccac acgctgtatc ggtacttcca gtgtgcatta
     2221 ttcttcggac atccgtttac acatactccg catccaatac acgcgccgcc tgccgtctgc
     2281 tttttctctg ccagatcaat tcccatggta cattttgcag tacagagacg gcagtttccg
     2341 catttgctcc gcggttttac aatttttaac agagcacatc tggaaatcag gctgtagatg
     2401 gctcccatgg gacagagata ccggcagaaa aagcgctgga tcaaaacaga tccaacgaaa
     2461 atcaccagaa tcagcgcacc gccaatcgaa aacatcggct tcacacccgg ccagtgtccg
     2521 atggaactgt actgtccaaa cacctgccac ggccctgcga tgttccattt tatcataccg
     2581 ctccatacga agactgccca gaacaggagc acaccatatt tgatccagcc tgttggaaca
     2641 tttcttccac tcttctttga atttgaatgc aggtttttcg cacctgcttt tcgaaaccgc
     2701 tttttcagct tatttcctgc aaaccacaga aaatcctgca cagccccgaa gctgcagaaa
     2761 tatccgcaga aaaaccttcc cacaataatc accgccggaa ccgtcgccag cagcatccat
     2821 accgaatacc tgatccgttc ccacgaaacc gtcccctgat aaactgcgga aataatcgtc
     2881 ccaaccgcag aaaactgcgc ctcaaacaag cccgggatca gcaggaaaga aaggatctga
     2941 atcactgctt tcggccagtt tcttttgtct tgttttttca aatttgttcc tcttttctta
     3001 tgtttaacgt ttcatctcca ttaccggacc attcgattta tgttcctgga ttaattcaca
     3061 aatctccgaa agattcattg ccgggaaatc tccggggacg gtatccttca gggcacttac
     3121 tgcatttccg atggatactg cctgtgacag taaaaaaccc tcataaatcg tgtattttct
     3181 gcacatttat gagggtttta tatttttgcg ttatttttac aaattcacag ttgaattatg
     3241 cgttcatctg tttagcaact tcctcagcga agttctcttc tttcttctcg attccttcac
     3301 cggtctcgaa acgaacgaag ccttttactg ctacggttgc gttgtttgct ttagcaacct
     3361 gtgctacgta ctgagctacg gactgttttc cgtcttctgc ttttacgtat acctggtcaa
     3421 gcaggcagat ctctttgagc tctttgttga tacggccctg gatcattccc tggataactt
     3481 tttccggttt ctgggactct ttcgggtcgt tctgaatctg agccatgagg atttctttct
     3541 catgtgcgat gtaatcagcg cttacttcgt cgcggtttgt gtactgcgga cgaagagctg
     3601 caatctgcat tgcaacgttt ttagccatct ctttgaggtc atcgtttaca acatctgtct
     3661 caacgtcaac cagaacaccg attttaccgc ccatatgagt ataagaagca acgaaaccgt
     3721 tctcctcttt tacctgctgg aaacgacgga tgttcatgtt ctcgccgatg gtagcgatct
     3781 gatgagccag ctcttcgcca acggtctcgt tggtatcgaa tttccaaggc tctgccagga
     3841 atccttctac gtctgctgca gttgtatcca tagcctgctc tgcaacctgt gctacatagc
     3901 cacggaattt ctcgttcttt gcaacgaagt ctgtctcagc gtttacttct acagcgacag
     3961 ctttctttcc atcctcggat acgttcagca taacgatacc ctcagctgcg attctgccag
     4021 cttttttctg agcagtagca agtccttttt ctctcaggaa ctccattgct ttgtccatat
     4081 caccttcggt agctgtaaga gctttcttgc agtccatcat accagcaccg cttacttctc
     4141 ttaactcttt taccattgct gctgtaatag ccattgtttt attcctccgt atacgtgatt
     4201 cagcagactg catcttcatg ccggtctgct attgtgagat tcgccgaagg gaacctgaat
     4261 ttcaagttcc ccccagttca ttacttcgcg taaattattc agcgtctgtc tctgctgctt
     4321 cttcgtatgc agcttcctca gcctcgccct gttttgcctc gataactgcg tctgccattt
     4381 tggaaacgat cagttttacg gcacggatag cgtcgtcatt acccggaatt acatagtcaa
     4441 gctcttccgg atcgcagttg gtatcgcaga taccgatcag cggaattccc agtgtatgag
     4501 cttcctgtac gcagattctc tcttttttcg gatctactac gaagatagcg tccggcagtc
     4561 tcttcatttc tttgataccg ccaaggtttt tctgcagttt tgcaagctct ttctttaact
     4621 caatgacttc ttttttcgga agaacttcga aggtgccatc ctgctccatg gtctcgattg
     4681 ctttcagtct tgcaatacgg ctctggatgg ttttgaagtt ggtcagcata ccgccgagcc
     4741 atctctcgtt tacatagtac attccacagc gctctgcctc aacagcgata gcgtcctgag
     4801 cctgtttttt tgttcctacg aaaaggattg tgccgccgtc tgctgcgatg tctgcaacag
     4861 ctttgtatgc gtcatctacc attcctacag atttctgcag gtcgatgatg
//
LOCUS       DAOMUF010000154         4889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4822_length_4889_cov_4.218246, whole genome shotgun sequence.
ACCESSION   DAOMUF010000154 DAOMUF010000000
VERSION     DAOMUF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4822_length_4889_cov_4.218246"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..831
                     /locus_tag="PLN77_08475"
     CDS             136..831
                     /locus_tag="PLN77_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44337.1"
                     /translation="MPGFILHLTAAQMLLKHLPSHPDFPYPISSVNDFLIGNLLPDAT
                     QQKESSHFRDPLYREKMMVFPDLTRFTAKYRSLLPDSSALGYYFHLYIDRKFFKDFIP
                     QIVEFYNADGEITDMRDEIATVYIKKSRTSIPFSRYLTEEYYYGDYTRMNTYLVNRYC
                     IPLDLNPNVTNPGIAEIQYENVQQVLDLLHHFLSVPPEAAQDLKVFPLEELLAALEQY
                     VEEFLAVPNKYIS"
     gene            complement(906..1844)
                     /locus_tag="PLN77_08480"
     CDS             complement(906..1844)
                     /locus_tag="PLN77_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM44338.1"
                     /translation="MEFNTFYNKIQTDESMMETIQHGNSEYPFQLYYDDLALFDFHCV
                     EWHWHVEFEFVFVQEGTATFWVGEKQFSLGQGTGIFINSKILHRYFSEGHAIVPNFVL
                     KPTFLAPADSLIYKKYIAPIQACKVDCIVFCREIKWQAEALELMQKILKAQESDKDRE
                     LVTLFLTQELWHLLYLHMDSEVLQKKRENTASSQGRTQLMMQYIHQNFRQNLTLEDIA
                     GQAMVSKSTALNLFRRYLHDTPVHYLVKYRLQEAAKLLATTEKKITVISGETGFENMD
                     YFCKTFKKYYGRTPTEYRRKKQMDTDDREWIVQDVV"
     gene            2052..3236
                     /locus_tag="PLN77_08485"
     CDS             2052..3236
                     /locus_tag="PLN77_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM44339.1"
                     /translation="MKKNYHKTLIACYLGFITQAISANFAPLLFLTFHRTYHISLGKI
                     AFISTIFFFTQLLVDLFCARYVDKIGYRRCVVASEILSACGLIGLAFLPNILPNAYAG
                     ILLSAMIYAAGSGLIEVLVSPIVEACPFDNKDSVMSLLHSFYCWGSVGVILLSTAFLA
                     VFGMERWPILACIWAVLPLYNTFNFLSCPIESLTGSEEGLTIRQLCRLPIFWISLVLM
                     VCAGASEISMAQWASAYAESALGLSKSIGDIAGPCLFAVMMGISRTFYGKYGEKIDLT
                     KFMIASGTLCLICYLAAALAPYPLLNLIGCVVCGFSVGIMWPGTISISSSKIPLGGTA
                     LFALLAMAGDMGGSIGPFVIGFVTQNTGDDLKAGMLAGCIFPAVLILSVFLLRQRNAE
                     QK"
     gene            complement(3299..4753)
                     /locus_tag="PLN77_08490"
     CDS             complement(3299..4753)
                     /locus_tag="PLN77_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRM44340.1"
                     /translation="MKKEELFETLGNLDPGMVENAGKYQRSRRAAWKKWTATAACAVI
                     IGGAVLGVMTWRNGREGSAVRYPSGVTTVLAAYPASVERTMDAQKFMESDAHWDWWDS
                     YRELTAKSAELQSGMDAYYQNLMKQMLVSEDENTVCSPINLYIAFAMLAETSDGNTRQ
                     QILDMLGAQDMDTLRKNISSLWKSNYADTPALKSVLANSLWLDGEETYNDTTLQRLAE
                     QYYASTFRGTPGSEEMNQALRTWTDDNTGGLLKEYTKDMAIAPETVFELVSTIYYKAM
                     WRENFWEVDTEKETFHGAAGDTTVDMMKKTEWMDVYQGEHFRAVSLSLQDSGSMYFLL
                     PDENTDVNELVSDPDLMKVIRRDESSDNWYSPMVNLSVPKFKVSEKTDLIETVRALGV
                     TDALDADLADFSPLTGDKENLYLSKADHAAMLEIDENGVTGAAYTELGVSETAAEIPD
                     DEIDFVLDRPFLFLVTGQDGSILFSGVVRNIAET"
BASE COUNT         1185 a         1268 c          964 g         1472 t
ORIGIN      
        1 accggcaaat ttccatgtca agaaatttgc cagtgtttat gtttttttta gaatttacgc
       61 tactcaaata gaatttgaca aacgctgcca ttttgtttag attagaagca aatacgaaag
      121 gacaccttca ttcccatgcc aggttttatt ttacatctca ccgcagccca aatgctgtta
      181 aagcacctcc cgtcccaccc ggattttcca tatccgattt catcggtaaa tgattttctg
      241 atcggaaatc ttctgccgga tgcgacgcag cagaaagaat cctcccattt ccgcgatccg
      301 ctttaccgcg aaaaaatgat ggtattcccg gatcttacac ggtttacagc gaaataccgt
      361 tcacttctgc cagattcgag cgcactggga tattattttc atctttatat tgaccggaaa
      421 ttttttaagg atttcattcc gcagattgtg gaattttata atgcggatgg agaaattaca
      481 gatatgagag atgagattgc aacggtttat atcaaaaaat cgcggacatc cattcctttt
      541 tccagatatc tcacggagga gtattattac ggggactata cacgaatgaa tacatatctt
      601 gtcaaccgct attgcatccc gctcgatctc aatcctaacg tcacgaaccc tggaatcgca
      661 gaaattcagt atgagaacgt ccaacaggta cttgacctcc tccatcactt cctctccgtc
      721 ccgccggaag ccgcgcagga tctgaaggtc tttccgcttg aagaactgct tgcggcactg
      781 gaacaatatg tagaagagtt tttggctgtg ccgaataagt atatttcata attctccatt
      841 caaaaaggag cagaacccac accagattcc gctccttttt cagaatgtaa tttttccgtt
      901 tcctcttaca ctacatcttg aacgatccat tctctgtcat ccgtatccat ttgttttttt
      961 cttcgatatt ccgttggtgt cctcccataa tatttcttaa atgtcttgca aaaataatcc
     1021 atattttcaa atcccgtctc gccggaaatt acagttatct ttttctccgt cgtggccaga
     1081 agctttgcag cctcctgcag acgatacttc accagataat gcaccggcgt atcgtgaaga
     1141 tatcttcgga acagattcaa tgccgtactt ttgctcacca tagcctgccc ggcgatatcc
     1201 tccaatgtaa gattctgtcg gaaattttga tgaatatatt gcatcatcag ctgtgttctt
     1261 ccctgtgagg atgccgtatt ttctcttttt ttctgcagca cttccgaatc catgtgcaaa
     1321 taaagcaagt gccacaattc ctgcgtcaga aataaagtaa ccagttcccg gtctttgtct
     1381 gattcctgcg ctttgagaat tttctgcatg agctccagtg cttctgcctg ccattttatt
     1441 tccctgcaaa agacaatgca gtccacctta cacgcctgta tcggtgcaat gtattttttg
     1501 taaatcaggc tgtctgccgg agcgagaaat gtcggtttca gaacgaaatt tggcacaatc
     1561 gcatggcctt cagaaaaata gcgatgcaaa atctttgagt taataaaaat gcccgttccc
     1621 tgtcccagtg aaaactgctt ttcgccaacc cagaaggttg ctgttccctc ctgtacaaaa
     1681 acaaactcaa actccacatg ccagtgccat tctacacaat gaaaatcaaa caacgcaaga
     1741 tcgtcgtaat acaattgaaa cggatactca ctgtttccat gctgtatcgt ttccatcatg
     1801 ctttcatccg tttggatctt gttataaaat gtattaaatt ccatctttcc ccgcctccgt
     1861 ttttcaggca cgcccctctc tattttacga tattatacaa taaatagacg atatattcca
     1921 gtgaaagcga cactatttta cgttattata caaaaagaat cgttgtcgct ccatggaaca
     1981 cggtgaacgg caacgaaacg aacactacgg ccgcagcagt tctgccattt ttaaaaacgg
     2041 agtatatcac tatgaaaaag aactaccaca aaaccttaat tgcctgttat cttggattta
     2101 tcacgcaggc aatttctgca aattttgcac ctttactatt tcttaccttt cacagaactt
     2161 accatatttc tcttggaaaa attgcgttta tctcgacaat attctttttc acacaacttc
     2221 ttgtcgattt gttctgtgcc agatatgtcg ataaaatcgg ttatcgcagg tgtgtcgttg
     2281 cttctgagat tctttccgcc tgcggcctga ttggacttgc ctttctgccc aatattctgc
     2341 caaatgccta tgcaggaatt ttgctcagtg ccatgatcta tgccgccgga agcggactga
     2401 ttgaggttct tgtcagccca atcgtagaag cttgtccgtt tgacaataag gacagcgtta
     2461 tgagtctgct tcattccttt tactgctggg ggtctgtagg cgtcatctta ctctccactg
     2521 cttttttggc agtttttggt atggaacgct ggccgatcct cgcatgtatc tgggctgtac
     2581 ttccactgta caatacgttt aatttccttt cctgcccgat tgaatcactc accggaagcg
     2641 aagaaggact taccattcgt cagctgtgcc gtcttcccat tttctggatt tcccttgttc
     2701 ttatggtctg cgccggagca agcgagattt cgatggcgca atgggcttcc gcttatgccg
     2761 agtctgcatt aggtctctca aaaagcattg gagacattgc aggaccgtgc ctgtttgctg
     2821 tgatgatggg catcagcaga accttctatg gtaaatacgg ggaaaaaatt gacttaacaa
     2881 aatttatgat cgcatccggt acactttgcc tgatctgtta tctggctgca gctctcgcac
     2941 catatcctct tttaaatctt ataggctgcg ttgtgtgtgg tttctctgta ggaattatgt
     3001 ggccgggaac catcagtatt tcatcctcta aaattccact cggcggaact gcactgtttg
     3061 cacttctcgc catggccgga gatatgggcg gttccattgg cccatttgtt attggatttg
     3121 tcacacaaaa caccggcgac gatctgaagg caggtatgct ggccggctgc attttccctg
     3181 ctgtactgat tttgtctgtt tttctgctcc gacaaaggaa cgcagagcaa aaataagcac
     3241 cgaagagata ctgtaacgtg tctcagacac aggagccgga cagtccggct cctttttttc
     3301 atgtctctgc aatatttctc acaactcccg aaaacagaat cgatccgtcc tgccccgtga
     3361 caagaaacag gaatggacga tccagtacga agtcgatttc gtcgtccggt atttctgctg
     3421 cggtttctga aacgccgagt tctgtatacg ctgctccggt tactccgttt tcgtcgattt
     3481 caagcatggc ggcgtggtct gctttgctca ggtataaatt ctctttgtcc cctgttaatg
     3541 ggctgaaatc tgccagatcc gcatccagtg catccgttac acccagtgcg cgcaccgttt
     3601 cgatcaggtc tgttttttcc gatactttaa atttaggaac agacaggttc accatagggg
     3661 aataccagtt atcactgctt tcatcccgcc gtataacctt catcagatcc ggatccgata
     3721 ccagttcatt gacatcggta ttttcatccg gaagcaggaa atacatggag ccgctgtcct
     3781 gaaggctaag gctgactgca cggaaatgtt cgccctggta gacatccatc cattccgttt
     3841 ttttcatcat gtccacagtg gtatctcccg cagctccgtg gaaggtttcc ttctctgtat
     3901 ctacttccca gaaattttcc ctccacatcg ccttataata gatggttgat accagttcaa
     3961 ataccgtttc cggggcaatc gccatgtctt ttgtatattc cttcaaaagt ccgccggtat
     4021 tatcatccgt ccacgtccgc agggcctgat tcatctcttc cgatccggga gtcccacgga
     4081 aggtcgatgc ataatactgt tccgccagcc gctgcagcgt tgtgtcattg tatgtttcct
     4141 caccatccag ccacagcgaa tttgccagca cacttttcag cgcgggcgtg tctgcataat
     4201 tgcttttcca gagcgaagaa atatttttcc gtaaggtgtc catatcctgc gcaccaagca
     4261 tatccagaat ctgctgccgc gtattcccgt ctgaagtttc tgccagcatc gcaaacgcaa
     4321 tatacagatt gatcggtgag cagacggtat tttcatcctc tgaaaccagc atttgtttca
     4381 tcaggttctg ataatatgca tccattcccg actgcagttc tgctgatttt gctgtcaact
     4441 cccggtacga atcccaccag tcccagtgtg catcgctttc catgaatttc tgtgcatcca
     4501 ttgtccgctc cacagatgcc ggataagccg ccagaaccgt cgttaccccc gacggataac
     4561 ggactgcaga accctcccga ccgtttctcc atgtcataac ccccagcaca gctccgccaa
     4621 tgatcaccgc acaggctgca gtggccgtcc attttttcca tgcagccctt cgtgaacgct
     4681 gatatttccc cgcgttttcc accataccag gatccagatt tcccagcgtt tcaaataatt
     4741 cttctttctt catacttcga accccctttc cgtcaagtgt gtatgcagtt tcccgcggat
     4801 ccggttcagc gtaactgaaa catagttttc acttcttccc agccgatccg cgatttcacc
     4861 gatactgtct acataccagt accgcagga
//
LOCUS       DAOMUF010000155         4790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4960_length_4790_cov_3.456811, whole genome shotgun sequence.
ACCESSION   DAOMUF010000155 DAOMUF010000000
VERSION     DAOMUF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4960_length_4790_cov_3.456811"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..726
                     /locus_tag="PLN77_08495"
     CDS             280..726
                     /locus_tag="PLN77_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM44341.1"
                     /translation="MKDAFEKLFRAVHQFKKLNVSDLIPGLSSSEFSVMGAILQMGEN
                     GKITSSELAAKTKTLPPAVSRTLRGLEEKGYVERSVDKKDRRNTYISLTEKGWKKGEE
                     VRDRMQDFGCSVMSQLKEEDIDQLVAYLDRIYEIAEKEIDTRKRQN"
     gene            772..3093
                     /locus_tag="PLN77_08500"
     CDS             772..3093
                     /locus_tag="PLN77_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44342.1"
                     /translation="MSKIFKNMIPYWKSILVILVLLFVQAWCDLSLPSYTSDIIDVGI
                     QNSGVEHVTPKRITEEEFQTAEFIMTDDEVDLWESLYTKKDGYYERNKASEKELDEAD
                     DTLTVALLMNYQMGAMEEDTFKQLMAQQTGQDASVFENMTIEQIGEMMHVELKSFQQE
                     KEDDDGNKVEVTCVDVRPIFAAMLESGQMDKDTVLSMRDSMEKTLDTMGSSLVKSMGI
                     AYAVSADKAAGLDVDQIQKTYLLTAGLKMVGMALLMGVVTVLVGLFASRVGAGIGRDL
                     REKVFKRVVGFSNAEMDQFSTASLITRSTNDIQQIQMVSVMVLRMVAYAPILGIGGVL
                     KVMKTGAGMEWIIVLAIIVILGYVMLLVSLAMPKFKLMQKLVDNINLVSREILTGLSV
                     IRAFGREDKEEERFDGANKELTKTTLFTNRVMTFMMPGMMMIMNVLTVGIVWVGAHKI
                     DAGTMQVGAMTAFITYAMMIVMSFLMLTMMSIMLPRAAVAAGRIDEVIQTESSIQDVK
                     NPEQLEVHNGVVRFDHVNFRYPGAEEDVLHDIDFVAEPGKTTAIIGSTGCGKSTLVNL
                     IPRLYDVTGGKITLDGKDIRNITMKDLRDEIGFVPQKGVLFSGTIASNLRFGKDDATD
                     AEIEKAAAIAQATEFIEAKDDKYETAIAQGGTNVSGGQKQRLAIARAIAKDPKIFIFD
                     DSFSALDLKTDAALRKALAENVKDSTVIIVAQRISTILHAEQILVLDDGKVVGKGTHE
                     ELLRSCEVYQEIAKSQLSEKELGLKESEVADHE"
     gene            3086..>4790
                     /locus_tag="PLN77_08505"
     CDS             3086..>4790
                     /locus_tag="PLN77_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44343.1"
                     /translation="MSNEMNQQPRKRGPMGRMGRGMQPGEKPKDLKKSIKQLAQYIGK
                     YKIAIFIVMICAACSTVFNVAGPKILGKATTALSEGLMEKIRGTGSIDFARIGTILLF
                     VLGLYLMSALFSFIQGWIMTGVTQKVCYQLRKEISEKINRMPMKYFESRTYGEVLSRI
                     TNDVDTLGQGLNQSITTIITSVATLIGVLIMMLSISPLMTLIALVILPISMGLIGLVT
                     KKSQKFFRAQQEYLGHINGQVEEVYGGHLVIKAFNREKDTIEEFEKTNNILYDSAWKS
                     QFLSGMMQPIMMFVGNLGYACVALTGGLLAIKNVITIGDIQAFIQYVKNFTQPIQQIA
                     QVINMVQSMSAASERVFEFLNEEEEDQTTENPADIETVTGAVTFDHVQFGYNPDQIII
                     HDFCAKVKPGQKIAIVGPTGAGKTTMVKLLMRYYDVNSGAILLDDHNIRDFNRRELRD
                     AFGMVLQDTWLFKGSIMENIRYGRLDATDEEVIAAAKAARAHHFIQTLPGGYQMELNE
                     DASNVSQGQKQLLTIARAILADNKILILDEATSSVDTRTEIEIQKAMDNLMKGRTSFV
                     IA"
BASE COUNT         1356 a         1064 c         1319 g         1051 t
ORIGIN      
        1 gaatatattg ttcataatgg aatgatgaaa gatctttctg tgctccagga agcgccgttt
       61 acagatcggg gaagtgttgc ggaagtcttt acagatctgg cagtctggat gggcattcgg
      121 aaagtaatca atcagattaa cgaaaatgcg gtagcgtaaa ttctaaaaaa aacataaaca
      181 ccggcagatt tcttgacata gaaatttgcc ggtgttatta tttaacacgc gtaaataaat
      241 aacactgtta agcatttgcg gcaaaaggag ggggaaaaca tgaaagatgc atttgaaaaa
      301 ctgtttcgtg cggtgcacca gtttaaaaaa ctgaacgtat cagacctgat tccgggactc
      361 agcagttccg aattttccgt tatgggtgcg attttgcaaa tgggagaaaa cggaaaaatt
      421 acaagctcag agctggcggc gaagaccaaa acacttccgc cggctgtgtc gcgcacactg
      481 cgcggactgg aggaaaaagg atatgtagag cggagcgttg acaaaaaaga ccgcagaaat
      541 acgtatatca gtctgacaga gaagggctgg aaaaaggggg aagaggtacg ggacagaatg
      601 caggactttg gctgctctgt gatgtcacag ttaaaggaag aggatataga tcagctggtt
      661 gcttatctgg atcgtatcta tgagatcgca gaaaaagaaa ttgacacccg aaaacgacag
      721 aattagtgtg ccggcaagct ggtacacaaa aaaagacaga aaggggaaaa aatgagtaag
      781 attttcaaga acatgatccc gtactggaaa tcgattctcg tgattcttgt gcttctgttt
      841 gtccaggcgt ggtgcgacct gtcgctcccg tcgtacacat ctgatatcat cgatgtgggc
      901 attcagaaca gcggtgttga gcacgttaca ccgaaacgaa tcacagagga agaatttcag
      961 acggccgagt ttatcatgac ggatgatgag gtggatttat gggaaagcct ctatacgaaa
     1021 aaggacggct attatgaacg gaacaaagct tctgaaaaag aacttgatga agcagacgat
     1081 acactgaccg ttgcactgct gatgaattac cagatgggtg cgatggagga ggataccttt
     1141 aaacaactca tggcacagca gaccggtcag gatgcgtcgg tgtttgaaaa catgacgatt
     1201 gagcagatcg gcgagatgat gcatgtggag ctgaagagct tccagcagga aaaagaggat
     1261 gacgatggaa acaaagtcga agtcacctgt gtggatgtcc gtccaatctt tgcagcgatg
     1321 ctcgagagcg gacagatgga taaagacacc gtgctctcga tgcgcgacag catggaaaaa
     1381 acgcttgata cgatgggaag ctccctggta aaatcgatgg gcattgcgta tgcagtttcc
     1441 gcagacaaag cggcgggcct cgatgtagac cagattcaga aaacgtacct tctgaccgca
     1501 ggcctcaaga tggtcggcat ggcactcctg atgggtgtgg taactgttct cgtcggactg
     1561 tttgcctccc gcgtcggcgc cggaatcggc cgcgatctgc gcgaaaaagt attcaaacgg
     1621 gttgtcggat tctccaatgc ggagatggat cagttctcca ccgcatcgct gattacccga
     1681 agcacgaacg atatccagca gatccagatg gtctccgtca tggtgctgcg aatggttgcc
     1741 tacgctccga tccttggcat cggcggtgtg ctgaaagtca tgaaaacggg agccggtatg
     1801 gaatggatca ttgttcttgc gatcatcgtc attctcggct acgtgatgct gcttgtctcc
     1861 cttgcgatgc cgaaattcaa gctgatgcag aaactggtcg acaacatcaa cctcgtatcg
     1921 cgtgaaatcc tgaccggact ctctgtcatc cgtgcgttcg gaagagagga caaagaggaa
     1981 gagcgcttcg acggtgcgaa taaggaactg accaaaacaa ccctgttcac caaccgcgtc
     2041 atgaccttta tgatgcctgg tatgatgatg attatgaacg tgctgaccgt cggaatcgta
     2101 tgggtcggtg cacataaaat cgatgccggc acgatgcagg tcggtgcaat gactgcattt
     2161 attacctacg ccatgatgat cgtcatgtct ttcctgatgc tgacaatgat gtcaatcatg
     2221 ctgccgcgtg ccgctgttgc cgccggccgt atcgatgaag tcattcagac agaatcctcc
     2281 attcaggatg tcaaaaatcc ggagcagctg gaggttcaca acggagttgt ccgcttcgat
     2341 cacgtcaact tccgttatcc gggcgccgag gaggatgtgc tgcatgacat cgacttcgtt
     2401 gccgagcctg gcaagacgac ggcgatcatc ggaagcaccg gctgcggtaa atcgacgctg
     2461 gtcaacctga tcccacgtct ctatgatgtg acgggcggaa agattacgct cgacggaaaa
     2521 gatattcgaa acattacgat gaaggatctg cgcgatgaga tcggtttcgt gccgcagaaa
     2581 ggtgtgctgt tctcgggaac gatcgcatcc aacctgcgct tcggaaaaga cgatgcgacg
     2641 gatgcggaga tcgaaaaggc agccgcaatt gcgcaggcaa ccgagtttat cgaggcaaaa
     2701 gatgacaaat acgaaaccgc gattgcacag ggcggaacga acgtatccgg cggacagaaa
     2761 cagcgtctgg cgattgcgcg tgcgattgca aaagacccga aaatctttat tttcgacgac
     2821 agtttttctg cacttgacct gaaaacggat gcggcgcttc gaaaagcact ggcggagaat
     2881 gtcaaagaca gcaccgtgat catcgtggca cagcggatca gcacgattct ccatgcggag
     2941 cagattctgg tgctcgatga cggaaaagta gtcggaaaag gtacgcatga ggaactgctg
     3001 agaagctgcg aggtttacca ggagattgcg aaatcccagc tgtcagagaa agagcttgga
     3061 ctgaaggaaa gtgaggtggc tgaccatgag taatgaaatg aaccagcagc cgcgcaaaag
     3121 aggaccgatg ggacggatgg gccgcggcat gcagccgggc gagaagccga aagatctgaa
     3181 aaaatcgatc aaacagctcg cccagtacat cggaaaatat aaaattgcga tttttatcgt
     3241 tatgatctgt gccgcatgct caacggtgtt caacgttgcc ggaccaaaga ttctcggaaa
     3301 agctacgacc gcactgtccg agggactgat ggaaaagatc cggggcacag gaagcattga
     3361 ttttgcaaga atcggaacga ttctgctgtt tgtacttggc ctgtatctga tgagcgccct
     3421 gttcagcttc atccagggat ggatcatgac gggcgtgacg cagaaggtgt gctatcagct
     3481 tcgaaaagag atttcggaga agatcaaccg tatgccgatg aaatattttg agagccgcac
     3541 gtacggtgaa gtgctctcac gcatcacaaa cgatgtcgat acgcttggcc agggactgaa
     3601 ccagagtatc acgacgatca tcacctcggt ggcaacgctc atcggtgttc tgatcatgat
     3661 gctgagtatc tccccattga tgacgctgat cgcgcttgtg atcctgccga tttcaatggg
     3721 actcatcgga ctcgtgacaa agaaatcaca gaaattcttc cgtgcccagc aggaatactt
     3781 aggccacatc aacggtcagg ttgaggaggt ttacggcggc catctcgtca tcaaggcatt
     3841 caaccgcgag aaggatacga tcgaagagtt tgaaaaaacc aacaatattc tttacgattc
     3901 cgcatggaaa tcccagtttt tatccggtat gatgcagccg attatgatgt ttgtcggaaa
     3961 tttaggctac gcctgcgtgg cattgaccgg cggacttctg gcgatcaaaa acgtgatcac
     4021 gatcggtgat atccaggcat ttatccagta tgtgaaaaac tttacccagc cgatccagca
     4081 gattgcgcag gttatcaaca tggtacagtc gatgtcggcg gcatccgagc gtgtctttga
     4141 attcctgaac gaagaggaag aggatcagac cacggaaaat ccggcggaca ttgagacggt
     4201 aacaggagca gtaacctttg accatgtaca gttcggatac aatccggatc agatcatcat
     4261 ccatgatttc tgtgcgaagg taaaaccggg tcagaaaatt gccattgtcg gcccgactgg
     4321 agcaggaaaa acgacaatgg ttaagctgct gatgcgttat tatgatgtaa acagcggagc
     4381 cattcttctg gatgaccaca atatcaggga tttcaaccgc agagagcttc gcgatgcctt
     4441 tggtatggtg ctgcaggata cgtggctgtt caagggcagt atcatggaaa atatccgcta
     4501 cggccggctc gatgccacgg atgaggaggt tattgcagcg gcgaaggctg caagagcaca
     4561 ccatttcatt cagaccctgc cgggcggcta ccagatggag ctgaatgagg atgcaagcaa
     4621 tgtatcccag ggacagaaac agcttctgac gatcgcaaga gcaattcttg cagacaataa
     4681 gatcctgatt ctcgacgagg cgacctcatc cgttgatacc agaacagaga tcgagatcca
     4741 gaaagcaatg gataacctga tgaagggcag aacaagcttt gtcatcgccc
//
LOCUS       DAOMUF010000156         4778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_4971_length_4778_cov_3.721575, whole genome shotgun sequence.
ACCESSION   DAOMUF010000156 DAOMUF010000000
VERSION     DAOMUF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4971_length_4778_cov_3.721575"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PLN77_08510"
     CDS             complement(<1..1205)
                     /locus_tag="PLN77_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HRM44344.1"
                     /translation="MGKIFDYDGVPVLHTTSGDLKGYFYDGVYIYKGIPYAYADRFQM
                     PVPSKWDGVKDATNYGFVCPLQNQDTPNGELMVPHRYWPQDEHCQSLNIWTNKLDPEA
                     KKPVLVWFHGGGYAAGSSIEQVAYDGVSIAKKGDSILVSVNHRLNILGYLDLSPFGEK
                     YKNSANAGHADMVAALQWVHDNIALFGGDPENVTIFGQSGGGMKVIDLMQIPSADGLF
                     QKGLVMSGVMEDDPLGAGEKDGTEIITAMMKALGFDDVAQLETVPYPQLAAAYAKVAP
                     AIAQSGGYIGGGPKKGDYFYGNPFDAGFREHAHQIPMMIGTVYGEFATFAPAAYDKNK
                     LTAEEILEILKKVYGDNAEKVLDAFKEAYPEKNGVDVLAIDRAMREPTVKLAKLFAKG
                     GGKAYLYNFA"
     gene            complement(1371..3869)
                     /locus_tag="PLN77_08515"
     CDS             complement(1371..3869)
                     /locus_tag="PLN77_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM44345.1"
                     /translation="MERDNRDEDMMLKGTKQEMPGFRFRLLRPEKKRELWNPDFELLF
                     LLRGSGRVSYEDGEVHNLPMGSIFAINRFQICDLDLDEDGLALSLCVSAETIDSFHIK
                     LLKCEVKCQSFFYMEDQQEKFDLIRRDMARIFLEMYKNNEEQPIYMKSRVTALLEDLL
                     SYFTSGEKVEQGRGGRERIQQASDYILQHCREEITLEDLADHLYLSRAYISRSFPQYF
                     GVSFREYVTQVRLAHAVQEMHSGATLTEIAYHNGFVNETAMIRAFRKYRGMTPSEYRK
                     QMEYTVKQREKKGKEREEAAGDHDIFQSLLQYAAVTEQEIETTNESAVSVTAAVNGRK
                     PRVAGHWKRVINAGYAASVLNREVQDELEQLVQELGYELIRVKGILDDDMCVFRRNMW
                     GEIQFCWNYIDEVIDFILSTGAKPLLEFGHMPLLLAKTDPGRTMRPALSSSPRDLAEW
                     RMLIKNLMEHLRERYGINQMRRWIFNPWISGDVLTIDGGDEFFGTWKASYEEMKRVSP
                     DLVTCLSFGLGPEEHLKAFIETMKEKECVPEIFAFRSFGTVIYGEEEEKMNLIQNNES
                     VNMIVSRDPDFLRNRGEKVKGLLQKAGLGALPVLVDECSSNIWQRDLCNDTCYKAAWL
                     FKNLLENEEALQGIAYFSVNDRLDEVFPARETYHGGFGLFTMNGIPKAVCTALRLLGR
                     MGSRLVKRGDGYFISTEPEKNQSQIYLYNYVHYDMLYRYRHAVNISRTDRYRVFNMGE
                     IRTFSVKLTGLEPGKYCLRLYKVTKEHGSSYDAWVRMGAPERMNRMERAMLCHSADPE
                     YRVWEQETDPEGSLTVQERLEPHETALIEVEQIL"
     gene            complement(3982..>4778)
                     /locus_tag="PLN77_08520"
     CDS             complement(3982..>4778)
                     /locus_tag="PLN77_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM44346.1"
                     /translation="WGNTVALFDDYRDQLFAAQKIVDGRGMLCNFLENHDKTRIIDRF
                     LMPEDQNRYSEKMLPVTNFFLPGIVFLYQGQEIGMRDNPKQSIQGFVDKPTFAIYDRL
                     IAEGKTDAEALEQINRESREHSRTPMQWDASAEAGFTTGTPWFPVNKNYTELNYEAEE
                     KDPDSLLWFYRKMVAVRRREDLEETFLYGTLIPEYETVSGVLAYRRKDEKNNILILTT
                     SLADGITLPFTDTIEEVLLNNYDTCEAGEETIRLQPYQCLVLKVKE"
BASE COUNT         1052 a         1373 c         1038 g         1315 t
ORIGIN      
        1 gcgaagttgt agagatatgc tttgccgccg ccttttgcga acagtttggc aagttttacg
       61 gtcggctcac gcattgcacg gtcgatggca agaacatcta cgccgttctt ctccggatat
      121 gcttctttga aagcgtccag aaccttttcg gcattgtctc cataaacctt tttgaggatt
      181 tccagaatct cttccgctgt cagtttgttc ttatcataag ctgccggtgc aaacgttgcg
      241 aactcgccgt atacggttcc gatcatcatc ggaatctgat gtgcatgctc acggaagcct
      301 gcgtcgaatg gatttccata gaagtaatcg ccctttttcg ggccgccgcc gatgtagccg
      361 ccactctgtg caatcgccgg agccactttt gcatatgcag cagcaagctg cggatacggt
      421 acagtttcca gctgagccac atcgtcaaag ccgagcgctt tcatcattgc cgtaataatc
      481 tccgtgccgt ctttctctcc tgcgccgagc ggatcgtcct ccataacacc gctcatgaca
      541 aggcctttct ggaacaggcc gtctgctgat ggaatctgca tcaggtcaat caccttcatt
      601 ccgccgccgg actggccgaa aatggttaca ttctccggat cgccgccgaa cagggcgata
      661 ttgtcatgta cccactgcag ggctgccacc atatcggcgt ggcctgcgtt tgcggagttt
      721 ttgtattttt ccccaaacgg agacagatcc agatatccta aaatattcag tctgtggttg
      781 acagaaacaa gaatcgaatc acctttcttt gcgatgctga cgccgtcata cgcaacctgc
      841 tcaatggaag atcccgctgc gtatccaccg ccgtggaacc atacgagaac cggttttttg
      901 gcttccggat caagtttgtt tgtccagata tttaaggact ggcagtgctc atcctgcggc
      961 cagtagcggt gcggaaccat aagctcgccg ttcggtgtat cttggttctg aagcgggcat
     1021 acaaagccgt agtttgtcgc atccttcacg ccatcccatt tggacggaac cggcatctgg
     1081 aaacggtctg cgtatgcata cggaattcct ttgtaaatgt aaacaccgtc ataaaaataa
     1141 ccttttaaat cgccggatgt ggtatgcagc accggcacgc catcgtaatc aaatattttc
     1201 cccattttga aatacctctt ttctgtagaa tttagcattt atgttgtttt cagtataaac
     1261 aatattttcg tccttttcta ctcgaattgt cgcaagatct tcttatattg ttgctttttt
     1321 tccactcttt ctctgtattc tcccaccgcg agtgcaattt tttgaagatt ttacagaatc
     1381 tgctccacct caatcagcgc cgtctcgtgc ggctccaggc gttcctgcac cgtgaggctg
     1441 ccttccgggt cggtttcctg ctcccagaca cggtattccg ggtccgcact gtggcacagc
     1501 atcgcgcgct ccatccggtt catccgctcc ggagctccca tccgcaccca cgcatcgtag
     1561 ctgctgccgt gctctttcgt cactttatac agccgcaggc aatattttcc cggttccagt
     1621 ccggtcagct tcactgaaaa ggtccggatt tcccccatat tgaatacgcg gtaacggtcc
     1681 gtgcgggaaa tattcacggc atggcggtag cggtacagca tatcgtagtg aacatagttg
     1741 tacaggtaaa tctggctttg gtttttctcc ggctccgtgc tgataaaata gccgtcccca
     1801 cgcttcacca gacgagatcc cattctgccg agcagccgca gcgcagtgca gaccgccttt
     1861 ggaatgccgt tcatcgtgaa cagcccaaaa cctccatgat acgtttcccg cgccggaaat
     1921 acttcatcca aacggtcatt gaccgagaaa tacgcaattc cctgcagcgc ctcttcattt
     1981 tccagaagat ttttaaacag ccatgccgct ttataacagg tatcgttgca cagatccctc
     2041 tgccagatgt tgctgctgca ctcatccacc agaaccggca gtgcaccaag cccagccttc
     2101 tgcagcagac cttttacctt ctccccgcgg ttccgcagaa aatccgggtc gcggctcaca
     2161 atcatattga cgctctcgtt gttctggatc agattcattt tctcctcttc ctctccatag
     2221 atcaccgtgc caaacgagcg gaatgcaaaa atttccggca cacattcctt ttccttcata
     2281 gtttcgataa aggctttgag atgttcctcc ggtccaagac caaaggaaag acatgttaca
     2341 agatccgggc tcacacgttt catctcctcg tacgatgctt tccacgtccc aaagaactca
     2401 tctcctccat caatcgtgag cacgtctccg ctgatccacg ggttgaaaat ccagcgccgc
     2461 atctggttga ttccataccg ttcccttaga tgttccatca ggtttttaat cagcatgcgc
     2521 cactcagcca gatctcgcgg agagctgctt agcgccggac gcatcgttct tcccgggtct
     2581 gtttttgcca gcagcaacgg catatggccg aattccagca acggcttcgc tcccgtggaa
     2641 aggatgaaat cgatcacctc atcaatatag ttccagcaga actgaatctc gccccacata
     2701 ttccgccgaa atacgcacat atcatcatca agaattcctt ttacgcggat cagctcatag
     2761 ccgagctcct gcaccagctg ttccagctca tcctgcacct cgcggttcag cacacttgcc
     2821 gcatagccag cattgatcac gcgcttccag tggcctgcca ctcttggttt ccgtccgttt
     2881 actgccgctg tcacggaaac ggcactctca tttgtcgtct caatctcctg ctccgtgacc
     2941 gctgcatact gcaggagact ctggaaaata tcatggtctc ccgccgcctc ttcccgctct
     3001 tttcctttct tttcacgctg ttttactgta tattccatct gcttccggta ttcgctcggt
     3061 gtcataccgc ggtatttgcg gaatgcgcgg atcatcgccg tctcattgac aaatccattg
     3121 tgataggcaa tctccgtcag cgttgcgccg ctgtgcattt cctgtacggc atgcgccagg
     3181 cgcacctgcg tcacgtactc ccgaaatgat acaccgaaat actgcggaaa gctccgtgaa
     3241 atataggcac ggctcagata aagatgatcc gccagatcct ccagcgtaat ctcttcccgg
     3301 caatgctgca gaatatagtc tgatgcctgc tgaatgcgtt cccgtccgcc ccggccctgt
     3361 tccacctttt ccccggatgt aaagtaagaa agcagatcct ccagaagcgc cgtcacacgg
     3421 cttttcatat agatcggttg ttcctcatta ttcttgtaca tctcaagaaa gatccgtgcc
     3481 atatcccgcc ggatcaaatc aaatttttcc tgctgatctt ccatataaaa aaacgactga
     3541 catttgactt cacatttcag cagcttaatg tgaaatgaat caatcgtctc cgctgaaacg
     3601 cagagcgaca gagcaagtcc gtcttcgtcc agatccaggt cacagatctg aaaccggttg
     3661 atcgcaaaga tagatcccat cgggagattg tgaacttccc cgtcctcgta gctgactctt
     3721 ccactcccac gaagcagaaa taaaagttca aaatccggat tccacagttc ccgtttcttc
     3781 tccggccgga gtagccgaaa acggaatccc ggcatttcct gttttgttcc tttcagcatc
     3841 atgtcttcgt ctctgttgtc tctttccatt tcacacctca tgatagattt ttttgttgta
     3901 ttgtaccaca aaaaaggtcc cgcaaaaaga tcttctcctt ttgtgggacc aatttttcgg
     3961 tttttgtttt agtttttgca attattcctt caccttcagc accagacact gatacggttg
     4021 cagacggatc gtttcctcgc ctgcctcgca ggtgtcatag ttattcagaa gtacttcttc
     4081 gattgtatct gtaaatggca atgtaatgcc atccgcaaga gaagtagtaa ggatcagaat
     4141 attattcttc tcatccttcc tgcggtacgc cagaacgccg cttaccgtct cgtattccgg
     4201 aatcagcgtt ccgtagagga aggtttcttc cagatcttca cgtcgtctta ctgccaccat
     4261 tttgcggtag aaccacagca gggaatcagg atctttttcc tcggcctcat agttcagttc
     4321 cgtatagttt ttattcaccg ggaaccacgg cgtacctgtt gtgaatcccg cctcggcaga
     4381 tgcatcccac tgcatcggag ttctgctgtg ctcgcggctc tcgcggttga tctgctcgag
     4441 cgcttccgca tccgtttttc cttctgcaat cagacggtcg taaatagcaa aggtcggttt
     4501 gtcgacgaat ccctgaatag actgtttcgg gttgtcgcgc atgccaatct cctgtccctg
     4561 atacagaaat acaatacccg gcaggaagaa attggtgacc ggaagcatct tttcgctgta
     4621 gcggttctgg tcctccggca tcaggaaccg gtcgatgatg cgcgttttgt catggttttc
     4681 gagaaagttg cagagcatgc cgcggccatc cacgattttc tgcgccgcaa aaagctgatc
     4741 gcgataatcg tcaaacagcg ccacggtatt gccccatt
//
LOCUS       DAOMUF010000157         4735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5037_length_4735_cov_2.884829, whole genome shotgun sequence.
ACCESSION   DAOMUF010000157 DAOMUF010000000
VERSION     DAOMUF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5037_length_4735_cov_2.884829"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PLN77_08525"
     CDS             complement(<1..984)
                     /locus_tag="PLN77_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HRM44347.1"
                     /translation="MTDKKYTLGIDIGSTTVKIAILDEENQLLFADYERHFANIQETL
                     AHLLGEAHEKLGELTLQPVITGSGGLALANHLGIPFTQEVIAVSTSLKALAPQTDVAI
                     ELGGEDAKIIYFEGGNVEQRMNGICAGGTGSFIDQMASLLQTDATGLNEYAKHYQAIY
                     PIAARCGVFAKTDIQPLINDGATKEDLSASIFQAVVNQTISGLACGKPIRGHVAFLGG
                     PLHFLSELKTAFVRTLKLDDEHTIVPDNSHLFAAIGSAMNADGKTEISLLALKKRLEE
                     KVAIDFEVARLEPLFKDQAEYDEFLARQSVNNVVTADLSTYEGNCYLGIYAG"
     gene            1242..2378
                     /gene="tgt"
                     /locus_tag="PLN77_08530"
     CDS             1242..2378
                     /gene="tgt"
                     /locus_tag="PLN77_08530"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRM44348.1"
                     /translation="MYKILKMEGRAKRAHMETVHGNIETPVFMNVGTAAAIKGAVSTE
                     DLQQIGTQVELSNTYHLHVRPGDEVVKKMGGLHKFMVWDKPILTDSGGFQVFSLAGLR
                     KIKEEGVYFHSHVDGRKIFMGPEESMQIQSNLASTIAMAFDECPSSVADRRYVQNSVD
                     RTARWLQRCKDKMQELNQREDTINKHQLLFGINQGAIYEDIRIDHAKRISEMDLDGYA
                     VGGLAVGETHEEMYHILDEVVPYLPKEKPTYLMGVGTPANILEGVERGVDFFDCVYPS
                     RNGRHGHVYTNHGKMNLFNQKYELDPRPIEEGCQCPACRHYSRAYIRHLLKAKEMLGM
                     RLCVLHNLYFYNNMMTEIRNALDEGNFASYKRAKLAGFEEYDKK"
     assembly_gap    2440..2449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2450..2572
                     /locus_tag="PLN77_08535"
     CDS             <2450..2572
                     /locus_tag="PLN77_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRM44349.1"
                     /translation="LHNLYFYNNMMTEIRTALDEGSFASYKRAKLAGFEEYDKK"
     gene            2696..3040
                     /gene="yajC"
                     /locus_tag="PLN77_08540"
     CDS             2696..3040
                     /gene="yajC"
                     /locus_tag="PLN77_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRM44350.1"
                     /translation="MFLATTTAANSGVMSTGMLIMWLVIMFGMMYFLMIRPQKKEQKK
                     LQAMLNDLAVGDAVVTTSGFYGVVIDITDDDVIVEFGNNKNCRIPMRKTAITEVEKPE
                     TAVVEEKTEKKK"
     gene            3371..4456
                     /gene="leuB"
                     /locus_tag="PLN77_08545"
     CDS             3371..4456
                     /gene="leuB"
                     /locus_tag="PLN77_08545"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRM44351.1"
                     /translation="MEYRIGLIHGDGIGPEIVDEAKKVLDAVCEKYGHKFEYTNLLLG
                     GASIDVHGVPLTDETIEEAKKCDAVLMGSIGGDAKTSPWYKLSPDKRPEAGLLKIRKS
                     LGLFANLRPAYLYQELKDACPLKEEIIGEGFDMLIMRELTGGLYFGERSTVEENGIKK
                     ATDTLTYSEPEIRRIAIRAFDIARKRKKKVTSVDKANVLDSSRLWRAVVEDVAKDYPD
                     VTLEHMLVDNCAMQIVHNPCQFDVVLTENMFGDILSDEASMVAGSIGMLSSASLNETK
                     FGLYEPSHGSAPDIAGKNIANPIATVLSAAMMLRYSLDLDKEAEAVENAVQKILKDGY
                     RTVDIMSEGCKKVSTSEMGDLLVKALE"
     gene            4533..>4735
                     /locus_tag="PLN77_08550"
     CDS             4533..>4735
                     /locus_tag="PLN77_08550"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM44352.1"
                     /translation="MRSDAVKKGIAQAPQRSLMRALGLTEEEMKKPLVGIVSSYNEIV
                     PGHMNLDKITEAVKMGVAMAGGTP"
BASE COUNT         1416 a         1009 c         1259 g         1041 t
ORIGIN      
        1 accggcataa ataccgagat aacagttgcc ttcgtaggtg gaaagatcgg cggtcacgac
       61 gttgttgaca ctctggcgcg ccagaaattc gtcatactcg gcctgatctt taaacagcgg
      121 ctcaagacgg gcaacctcaa aatcgatagc aactttctct tcgagccgtt ttttcagtgc
      181 gagaagggaa atctcggttt ttccgtctgc attcatcgca gaaccgatgg ctgcaaacag
      241 gtgggagttg tccggtacga ttgtatgctc gtcatccagt tttaaggtac ggacgaatgc
      301 tgtctttaat tccgagagga agtgcagcgg gccgccgaga aaggcaacgt ggccgcggat
      361 cggtttaccg caggcaagac cagaaatggt ctggttgaca actgcctgga aaatggaagc
      421 ggaaagatcc tcttttgttg caccgtcgtt gatgagcggc tggatatcgg tttttgcgaa
      481 tacaccgcag cgtgctgcga tcggataaat cgcctggtaa tgcttcgcgt attcgttgag
      541 tccggttgca tcggtctgca gaagagaagc catctggtcg atgaaagaac cggtgccgcc
      601 ggcgcagata ccgttcatac gctgctccac attgccgccc tcaaaataga tgatcttggc
      661 atcctcaccg ccgagctcga tggcaacgtc ggtctgcgga gcgagtgctt ttaacgaagt
      721 ggaaaccgcg ataacttcct gcgtgaacgg gatgccgagg tggttggcaa gcgcaagtcc
      781 gccggaaccg gtgatgaccg gctgcagggt cagttcgccc agcttttcgt gtgcttcccc
      841 gagcaggtgg gcaagggttt cctgaatgtt tgcaaaatgg cgctcgtaat cggcaaagag
      901 aagctggttc tcttcgtcga ggatcgcaat tttaacagtg gtggaaccga tgtcgattcc
      961 gagagtatat tttttatcag tcatgaatca aactcctttt ataataagaa acaatctcaa
     1021 cacattatac gacaaaaaat aaggaaatac aattggaaaa agggcgatgt cttacgaaaa
     1081 aagtataaag tccttgtgaa aaatggagaa accaggaggg aaaaaggcag aaaaaagaaa
     1141 attcaggcat tgtcaaaaac gggttttgcc tgtatactaa aaagagtatg ccctgcatga
     1201 aaaacaggcg gcagggatca tgagaaagga tgaaaagcgt tatgtataaa attctgaaaa
     1261 tggaaggaag agcgaagcgc gcccacatgg aaaccgtgca cggcaatatc gagacgccgg
     1321 tattcatgaa tgtcggaacc gcggcagcga tcaagggagc cgtttccact gaggatctcc
     1381 agcagatcgg cacgcaggta gagctctcca acacgtacca cctccacgtg cgtccgggcg
     1441 acgaggttgt aaagaagatg ggcggactcc acaagtttat ggtctgggat aaaccgatcc
     1501 tgaccgactc cggcggattc caggtattct ctcttgcagg ccttcggaaa atcaaggagg
     1561 agggtgtcta tttccactcc cacgtagacg gaagaaagat cttcatggga ccggaggaga
     1621 gtatgcagat ccagtccaat ctggcatcca cgattgcgat ggccttcgac gagtgcccat
     1681 cgagtgtggc agaccgccgt tatgtgcaga attccgtgga ccgtacggca agatggctgc
     1741 agcgatgcaa ggataaaatg caggaactga accagagaga ggataccatc aacaagcacc
     1801 agctgctgtt tggtatcaat cagggagcaa tctatgaaga tatccgtatc gatcatgcaa
     1861 aacggatttc cgagatggat cttgacggat atgccgtcgg cggtcttgcg gtaggagaga
     1921 cacacgagga gatgtatcat attctcgatg aagtggtccc gtatctgcca aaagaaaaac
     1981 cgacatatct gatgggtgtc ggaacaccgg ccaatattct cgagggtgta gagcgcggcg
     2041 ttgatttctt cgactgtgta tatccgagcc ggaacggacg tcacggtcac gtttatacaa
     2101 atcatggaaa aatgaatttg ttcaaccaga aatacgaatt ggatccgcgc ccgatcgagg
     2161 aaggctgcca gtgtccggca tgccgccatt acagcagagc ttatatccgt catctgctga
     2221 aagcgaaaga gatgctcggc atgcgtctct gtgttctgca caatctgtat ttctacaaca
     2281 atatgatgac agaaatccga aacgcactcg atgagggcaa ttttgcctcc tacaaacgtg
     2341 cgaagcttgc cggatttgag gaatacgaca aaaagtaagc tctgttcaaa gccatgcgaa
     2401 atgcgagaag ccatattgag atgtggcgca gtgcaatcgn nnnnnnnnnc tgcacaacct
     2461 gtatttctac aacaatatga tgacagaaat ccggaccgca ctggacgagg gcagctttgc
     2521 ttcctacaaa cgggcaaaac tcgcaggatt tgaggaatac gacaaaaagt aagcgggaaa
     2581 gtatgaaatc tttggaaaat atccgaaaaa gacttgccca tcggttacgg attgtgtatt
     2641 atgataggtg tgtctaaaga acgggatgta ctctcgaata acaggaggaa agaaaatgtt
     2701 tttagcaaca acaacagcag caaacagcgg tgtgatgagc actggtatgt taattatgtg
     2761 gctggtcatc atgttcggta tgatgtattt cttaatgatc cgtccgcaga agaaagaaca
     2821 gaaaaaactg caggcaatgc tgaatgacct tgcagtcgga gatgcagtcg taacgacaag
     2881 cggtttctac ggcgtagtca tcgatatcac agatgacgat gtcatcgtag agttcggaaa
     2941 caacaaaaac tgccgtatcc cgatgcgcaa aacagcgatc acagaggttg aaaaaccgga
     3001 aacagcagtc gttgaagaga aaacagagaa gaaaaaataa acatgatcta ggtatgtaaa
     3061 aagcgcaggt attcaaaaaa aggatgagta cctgcgtttt ttttgcccat tagagatttt
     3121 atggaatttt cctgtagagt aaaaagcctt ccggaccgga ttgcccctgc ggcgagaccg
     3181 ggggaaaaga gggaaaaatg ccgcgtaatt ttcttttaaa cagcaaaaaa gcaaaaagat
     3241 tattgcgttt cgttagggat aaagaaattt cgtgttgaaa ttacggcgat aatagcgtat
     3301 aatttagaaa tgctatagac acagagaaaa tagaacggtt tttcagccgg aagaaaagag
     3361 ggagaaagat atggaatacc gtattggatt aattcacggt gacggaatcg gaccggaaat
     3421 tgtagatgag gcaaaaaagg ttctggacgc cgtctgcgag aaatatggac ataagtttga
     3481 atataccaac cttctgctgg gcggagcctc catcgatgtg cacggcgtgc cgctgacaga
     3541 tgaaaccata gaagaagcaa aaaaatgtga cgctgttctg atgggctcca tcggaggaga
     3601 tgccaagacc tccccatggt acaaattaag cccggataag cgcccggagg ccggactttt
     3661 aaaaatccgg aaatcgttag gcctgtttgc aaacctgcgc ccggcgtacc tgtatcagga
     3721 attaaaggat gcctgcccgt taaaagaaga aattatcggt gagggctttg acatgctgat
     3781 catgcgtgag ctgaccggcg gcctgtattt cggagagcgc agcaccgtag aagagaacgg
     3841 catcaaaaaa gcaacggata ccctgactta cagcgagcct gaaatccgcc gtattgcaat
     3901 ccgtgccttc gacatcgcca gaaagagaaa gaaaaaagta acaagtgtag ataaagcaaa
     3961 tgttctcgac tcctcccgtc tgtggagagc tgttgtggag gatgttgcaa aagattatcc
     4021 ggacgttacg ttagagcata tgctggttga taactgtgca atgcagattg ttcacaatcc
     4081 gtgccagttt gacgtggttc tgacggaaaa catgttcgga gatatccttt ccgatgaggc
     4141 aagtatggtg gcaggttcca tcggaatgct ttcttccgcc agcctgaatg agacaaaatt
     4201 cggtctgtac gagccaagcc acggctccgc accggatatc gcaggaaaaa acattgcaaa
     4261 cccgattgca acggttctgt ccgcggccat gatgctccgc tactctctgg atctcgataa
     4321 agaggcagag gcagtagaaa atgctgtaca gaaaatctta aaagacggat accgcaccgt
     4381 agatatcatg tccgagggat gcaaaaaagt atccacctcc gaaatgggag atctgctggt
     4441 aaaagcactg gagtgaggct tgcagattgc ggaaatggtt tttcagacac actctagcat
     4501 aaaactgtaa gataagaaaa gaggaaaaga tcatgagaag tgatgctgta aaaaaaggaa
     4561 tcgcacaggc accgcagcgt tctctgatgc gtgcgctggg tctgaccgaa gaagagatga
     4621 aaaaaccgct cgtaggaatc gtaagctcct acaatgagat tgtaccgggc cacatgaacc
     4681 tggataaaat tacagaggct gtaaaaatgg gcgttgccat ggcaggcgga acacc
//
LOCUS       DAOMUF010000158         4586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5269_length_4586_cov_2.132863, whole genome shotgun sequence.
ACCESSION   DAOMUF010000158 DAOMUF010000000
VERSION     DAOMUF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5269_length_4586_cov_2.132863"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PLN77_08555"
     CDS             <1..1201
                     /locus_tag="PLN77_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HRM44353.1"
                     /translation="QTDFVHASGTKEELQVANYLKEQCEALGVPAKLEGFRVAMGEIE
                     EAHLYADGMGIPCKALTCCGSGSVEGELYYMPGTDPVSIAGAKDKIVLMDTQGIGFFT
                     YQDLMKAGAKAILFQYGNSYYPHTDIDQRDLREAVVGEEKKVLCAMIHSAQAVELVKN
                     KVKQIRLEIRQKEYDGESHNVIAELPGKREEWIVLSAHYDTTSLSHGAYDNMSGCAGL
                     LGIMEQMKGKELNYGLRFVFCGSEERGLLGSKAYVRDHEKELRQTVLNINLDMIGTYM
                     GKFIACVSAEEKLTHYISYMAAEVGFPIAAENGVYSSDSTPFADHGVPAVSFARIANG
                     NVAPIHCRYDVKDVMSMEQLQKDIDFLTVFTGRFANAAVCPVAREIPETIRKQLDEYL
                     FRKRKEA"
     gene            1280..1774
                     /locus_tag="PLN77_08560"
     CDS             1280..1774
                     /locus_tag="PLN77_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM44354.1"
                     /translation="MKKIELLEIRQGNDALAEMVALFRVELRSYKGIASKPNVEDGRE
                     EMKEYLLAGFPVFAATVHGEYAGYVVCRIESEVVWVESIFVREEYRRQGVAAALHGKA
                     EEIAASYGNDTVYNYVHPNNHRMIEFLRKRGYTVLNLIEIRKPYQNEKLTQTITVGEH
                     EFDY"
     gene            complement(1743..2630)
                     /locus_tag="PLN77_08565"
     CDS             complement(1743..2630)
                     /locus_tag="PLN77_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM44355.1"
                     /translation="MELRQLEYFRAIVDAGTISGAARVLHMTQPPLSYQIKMLEEELQ
                     VPLLIRGTKKITLTEAGKTLYEQAGTLLMLADLTRREVVKSSQSATLHIGMTPSTVSL
                     LADYLLRFSRKYPHIHFDVHEGSTFALKDQLENHMVDLTTLRTPIVLNGCETKTLLKE
                     SITAMALPSHPLFAGRETLSLTELAEQPLILSNRYRKYMLSAFEQAGLFCDIYLTCED
                     ARTAMTMAEKGLGIALLPASMLNLTHKLKACTITDADLTTEILLAWRKGRLPSELRAF
                     LELWDENDSLNNQIHVPQP"
     gene            2754..4121
                     /locus_tag="PLN77_08570"
     CDS             2754..4121
                     /locus_tag="PLN77_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44356.1"
                     /translation="MKRIDFEHGTVTGNILGAALPMLVAQILSLLYNIVDRVYIARIP
                     EVGTAALGAVGLCFPLIVIITAFSNLFGSGGAPLFSIERGKNDQKKAERIMNTSYSMV
                     CVCAVILMVIGFLFARPLLSLFGASADAMVYAYPYMMIYLIGTLPSMVATGMNPFINA
                     QGYSTVGMLSVTIGAVANLLLDPLFIFVFGFGVQGAAIATVISQTLSAVFVLYFLTRK
                     SELKVRFVRKDEISECAGYAKNIVSLGTAGFIMQLTNSLVTICCNNVLSVTGGDIYIS
                     VMTIVSSVRQLVETPIYAMNEGTSPILSYNYGARRPARVRKAMCVMSAMILGYTAVMW
                     SVIVLAPHALIGIFSSDAVLTADAVPALKQYFAAFIFMDLQYIGQTVFKSLNKKKQAI
                     FFSLLRKVFIVVPLTYLMPYVLHMGTDGVFLAEPVSNVIGGSLCFITMLILVLPELKR
                     MEQQK"
     gene            4406..>4586
                     /locus_tag="PLN77_08575"
     CDS             4406..>4586
                     /locus_tag="PLN77_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44357.1"
                     /translation="MEENKMGTIPVSRLVISMALPLMLSLLINSLYNFVDSVFVARVS
                     EDALTAISLAAPMQLI"
BASE COUNT         1236 a          958 c         1229 g         1163 t
ORIGIN      
        1 ccagacagat tttgtgcatg caagcggcac aaaagaagaa ttgcaggttg caaactattt
       61 aaaggagcag tgtgaagcat taggagtccc ggcaaaactg gaaggtttcc gcgttgcgat
      121 gggcgaaatc gaagaggcac atttgtatgc agacgggatg ggaattccat gcaaagcgct
      181 cacatgctgc ggaagcggaa gcgtggaagg cgaactctac tatatgcctg gaaccgatcc
      241 ggtttctatt gccggtgcaa aagataaaat tgtgctgatg gatacgcagg gaattggatt
      301 ttttacgtac caggatctga tgaaggcagg cgcaaaagcg attctgttcc aatatggaaa
      361 tagctattac ccgcatacag atatcgacca gagagatctt cgggaagccg ttgtcggaga
      421 ggaaaagaaa gtgctctgcg ctatgattca ctccgcacag gcagtagaac ttgtgaaaaa
      481 taaagtaaaa cagatccgtc tggaaatccg gcagaaggaa tacgatggag aatcccataa
      541 tgtgattgca gaattgccgg gaaaaagaga agaatggatc gttttaagcg cgcattatga
      601 tacgacatca ctttcgcatg gcgcatatga caacatgagc ggatgcgcag ggcttcttgg
      661 aatcatggag cagatgaaag ggaaagagct gaattatggt cttcggtttg tattctgcgg
      721 aagcgaagaa agaggacttt taggatcaaa agcgtatgtc agagatcatg aaaaagaact
      781 gagacaaaca gtactgaaca tcaatctgga catgatcgga acgtatatgg gaaaatttat
      841 cgcctgtgta tcggcagaag aaaagcttac acattacatc agctatatgg ctgcagaagt
      901 tggatttcca attgcggcag aaaacggcgt ctattccagt gattccacac catttgccga
      961 tcatggggtt ccggctgttt cgtttgcgcg tattgccaac ggaaatgtcg ccccgattca
     1021 ctgccgctac gatgtcaaag atgtgatgtc gatggagcag ctgcaaaaag atattgattt
     1081 cctgacagta tttaccggaa gattcgcaaa cgcagcggtc tgcccggtgg caagagaaat
     1141 accggaaacg attcggaaac aattggatga atatttgttt cgcaagagaa aagaagcctg
     1201 atatacttcg atcacggata tgagcagcga gacgaattgc aaataggaaa aagaacgaca
     1261 gtggcaggag agagaaacca tgaaaaaaat agaactgtta gaaatcaggc agggaaacga
     1321 tgcccttgca gaaatggttg cgctgtttcg agtagaactg cgttcctata aaggaattgc
     1381 atcaaaaccg aacgtggaag acggacggga agaaatgaag gaataccttt tagcaggatt
     1441 tccagtgttt gccgccacgg tgcatggcga atacgcaggt tatgttgtct gtcgtattga
     1501 gagcgaggtg gtgtgggtgg aatccatctt tgtcagagaa gagtaccgcc ggcagggagt
     1561 cgctgccgca ctgcatggca aggcagaaga aattgcagct tcttatggaa acgatacggt
     1621 ttacaactat gtccatccca ataaccacag gatgattgaa tttttgagaa agcgtggtta
     1681 caccgttctg aatctaattg agatccgtaa accgtatcag aatgaaaaac tgacgcagac
     1741 gattacggtt ggggaacatg aatttgatta ttgagggagt cattttcatc ccacaattcc
     1801 agaaaagccc gaagttcaga cggaaggcgc cccttgcgcc aggcaagcag gatttcggtt
     1861 gtaaggtctg catctgtgat ggtgcaggcc tttaatttat gggtaagatt cagcatggag
     1921 gccggcagaa gagcgatgcc aaggcctttt tctgccattg tcattgccgt gcgggcgtcc
     1981 tcgcaggtga gatagatatc gcagaacaga cctgcctgct cgaaagcgga gagcatgtat
     2041 ttccggtagc ggttcgacag aatcagcggc tgctcggcaa gttccgtaag cgaaagggtt
     2101 tcacgcccgg caaaaagcgg gtgagaaggg agtgccatgg cggtgatgct ctcttttaaa
     2161 agggtcttcg tctcgcaacc atttaaaacg attggcgtgc ggagcgtggt gagatccacc
     2221 atgtgatttt cgagctggtc ttttaaagca aaggtagaac cctcgtggac atcgaaatgg
     2281 atgtgcgggt atttccggga aaatcgcagg agatagtcgg cgagcagcga aacggtggat
     2341 ggcgtcatgc cgatgtggag tgtcgcagac tgactggatt ttacgacttc gcgcctggtg
     2401 agatccgcaa gcatcagaag cgttcctgcc tgttcataca gtgtttttcc tgcctcggtc
     2461 agagtgattt tcttcgtgcc gcggatgaga agaggcacct gaagctcttc ctccagcatt
     2521 ttgatctgat aacttaacgg cggctgtgtc atatgcagca cccgtgccgc cccgctgatg
     2581 gttcccgcat ccacgatggc gcggaagtat tcgagttgtc gaagttccat aaaaatcctc
     2641 ctgaaaaaaa tatataaaaa ttatttaatt tcaatataaa aacaataatt tacatataga
     2701 atacgccatg ctatactgaa aagcaagtga aaaagaaggg aatgagggaa caaatgaaac
     2761 gaattgattt cgaacatgga accgtaaccg gaaacatcct cggggcagcg ctcccgatgc
     2821 tggttgctca gattttgagt ctgctgtaca atattgtaga ccgcgtctac atcgcgcgga
     2881 tcccggaggt aggaacggcg gcactcggtg cagtcggtct gtgctttccg ctgatcgtta
     2941 tcatcacagc attcagcaac ttgtttggaa gcggcggcgc tccgctgttt tcgattgagc
     3001 gtggaaagaa tgatcaaaaa aaggcggaac gtattatgaa tacgtcgtat tccatggtat
     3061 gtgtctgtgc tgtcatactg atggtgatcg gctttttgtt tgctcgtcca ctgctgtcgc
     3121 tgttcggtgc atcggcagat gcgatggtat acgcgtatcc ttatatgatg atttatctga
     3181 tcggaacttt gccgtcgatg gtggcaaccg gcatgaaccc gttcatcaac gcgcagggct
     3241 attcgaccgt tggcatgctt tccgtcacga tcggagcggt cgcaaacctg ctgcttgacc
     3301 cgctgtttat ctttgtattt ggttttggtg tgcagggggc tgcgattgca actgtgattt
     3361 cccagacact ttccgccgtg ttcgttctgt atttcctgac ccggaaatcg gaactgaaag
     3421 tgcgctttgt gcggaaagat gaaatttctg agtgtgcagg ctatgcgaaa aatattgtca
     3481 gtctcggtac cgccggattt atcatgcagc tgaccaacag ccttgtcacg atctgctgca
     3541 acaatgtact ttctgtgacc ggcggtgata tttacatctc tgttatgacg attgtatcga
     3601 gcgtgcgtca gctcgtcgag acaccgatct atgcaatgaa tgagggaact tctccaattt
     3661 tgagctataa ttacggtgca cgccgtccgg cccgcgtgcg caaggcgatg tgtgtcatga
     3721 gtgcgatgat cctcggctat accgcagtga tgtggagcgt gattgttctg gcgccgcatg
     3781 cgctgatcgg tattttcagt tcggatgcgg tactgacagc agatgcggtt ccggctctga
     3841 agcagtactt tgcagcattc attttcatgg atctgcagta cattggacag accgtattca
     3901 aatctctgaa taaaaagaaa caggcgatct ttttctctct gctccgaaaa gtcttcattg
     3961 ttgtaccgct cacctatctg atgccgtacg tactccacat gggaacggac ggtgtcttcc
     4021 ttgccgaacc ggtatcaaac gtaatcggcg gaagtctctg ctttatcacc atgctgattc
     4081 tggttctgcc ggaattgaag cgaatggagc agcagaagta atagttttga aaagcttttg
     4141 aaaaaagtac aggaagatat ttaccgaaat cgtttcggca gatatcttcc tgtttttgtt
     4201 gcgatacata cgataaaaag ggttagattc ccctttttcg ctgcgctgca ttcgaagaga
     4261 gctgccggtg gcagtttctg caggtgaaaa aattataaag tatagctaaa aactattttg
     4321 ctgtatcgaa gataagtatt tgatctttcc ggaaattcac gctacactgg aaacagcaaa
     4381 aacaaaagaa caggagaggg tttttatgga agaaaacaaa atgggaacga tcccggtcag
     4441 ccgtctggtg atttccatgg cgctgccgct gatgctgtca cttctgatta attcgcttta
     4501 caacttcgtg gattccgtgt tcgttgcgcg ggtttctgag gatgcactga cggcaatttc
     4561 cctggcggct ccgatgcagc tcattg
//
LOCUS       DAOMUF010000159         4582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5276_length_4582_cov_2.578087, whole genome shotgun sequence.
ACCESSION   DAOMUF010000159 DAOMUF010000000
VERSION     DAOMUF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5276_length_4582_cov_2.578087"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLN77_08580"
     CDS             complement(<1..646)
                     /locus_tag="PLN77_08580"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRM44358.1"
                     /translation="MAQIDLTKYGITGTTEIVYNPSYEQLFEEETKPELEGYEKGRET
                     ELGAVNVMTGIYTGRSPKDKYIVVDENSKDTVWWTSDEYKNDNHPATQEAWAAVKEIA
                     KKELSNKRLFVVDAFCGANKDTRMAIRFIVEVAWQAHFVTNMFIKPTAEELENFEPDF
                     VVYNASKAKVENYKELGLNSETAVVFNITSREQVIINTWYGGEMKKGMFSMMNYY"
     gene            961..2295
                     /locus_tag="PLN77_08585"
     CDS             961..2295
                     /locus_tag="PLN77_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44359.1"
                     /translation="MYYDLTRGKISRSLILFALPMIAGNLLQQFYNIADTLIVGRVLG
                     SNALAAVGSSYTLMTFLTSIFLGLSMGAGALFSIYQGRKDLSSLKLSILHAFLLILAV
                     TLVLNLAVYAGIEPILTFLRVPDEVRPGMRTYLLIIFAGLIATSVYNFFSCMLRALGN
                     SSVPLIFLAVSAVLNIALDLLFVAVLPFGIAGAALATVLAQYVSAVGILLYVLLRCRM
                     WLPSKKEWRFSHSILKEILNLSSMTCIQQSVMNFGILMVQGLVNSFGPAVMAAFAAAV
                     KIDTFAYLPVQDFGNAYSTFTAQNYGAGNHERIRRGTKDAFALSAAFSIVISLLVNLF
                     AHPLMQIFVKASEMEVIAIGVQYLRLEGSFYLGIGWLFLLYGYYRAVKRAEMSVVLTV
                     ISLGMRVALAYLFSATALGVAGIWIAIPIGWALADMTGLFFLRRAFRSQKPA"
     gene            complement(2306..3208)
                     /locus_tag="PLN77_08590"
     CDS             complement(2306..3208)
                     /locus_tag="PLN77_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM44360.1"
                     /translation="MLSQMNYRKETQPHGDELFPLSIHVFETDLQSTVRVYCHWHEEV
                     ELFYAVKGEAVFHMDTETFLLREGETAFVNANRLHEVEGIDGQEFTFLAVVFQPRFLS
                     GTSLDRIQQQYIEPVLSSEIRFPSKTERGTRLAEKTAQAMQEIRNLQERKEPGWELLL
                     KAELLKIWQGYYSAAEKNGGKTDASTNYRVSRMKEILTYMNAHFCEDLEIAELASQFH
                     MSESAFCRFFKSIMHISAVTYVNNLRIAESCRLLLSTEEAISKIASDCGFRNISYFNR
                     VFQERMHQTPGQFRSLSAENRTAI"
     gene            3498..3752
                     /locus_tag="PLN77_08595"
     CDS             3498..3752
                     /locus_tag="PLN77_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRM44361.1"
                     /translation="MIKTVLKIDGMMCGMCESHMNDVVRKSCSPKKVNSSAKNGETVV
                     ISEAPLDIPYLKQQIKDIGYELVSYTSEPYEKKGFFHFGR"
     gene            complement(3875..>4582)
                     /locus_tag="PLN77_08600"
     CDS             complement(3875..>4582)
                     /locus_tag="PLN77_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRM44362.1"
                     /translation="PLADTREEDYQTILAEIRELPDGPELSPTANAKSSTAAAEEVKK
                     KIAEPLDTMLNAVELAQYVESAKQFYGALEELETALGATRFLAGDFVTEADAALYVTL
                     VRFDLLYSRYLGPVKYRVQDLKNVSDYLKDLYQIPAFAHHTDFAAIIRQGRIEGEEDG
                     FRASTHYDLALPKIDWDAQWKVSTERAYLSSDPTHPIYLGNNRRFDIDPTWYDLGAES
                     PKKEEKETPPSCGCCCG"
BASE COUNT          982 a         1156 c         1056 g         1388 t
ORIGIN      
        1 gatagtagtt catcatggag aacatacctt tcttcatctc tccgccgtac catgtgttga
       61 tgataacctg ctcacggctt gtgatgttga atactactgc tgtctcagag ttcagaccca
      121 gctctttgta attctcaact tttgctttgg atgcgttgta tacaacgaaa tccggctcga
      181 agttctcaag ctcttctgct gtaggtttga tgaacatgtt agttacgaaa tgtgcctgcc
      241 aagctacctc aacgatgaag cggatagcca tacgggtatc tttatttgca ccgcagaatg
      301 catctacaac gaacagtctt ttgttggaga gctctttttt tgcgatctct tttactgctg
      361 cccatgcttc ctgggttgcc ggatggttat cgtttttgta ctcatcggaa gtccaccata
      421 cagtatcttt cgaattctcg tccacaacga tgtatttgtc tttcggtgaa cggcctgtat
      481 agataccggt cataacgtta actgcaccaa gctctgtctc ccggcctttt tcatagcctt
      541 ccagctctgg ttttgtctct tcctcaaata actgctcata agaagggttg tacacaattt
      601 ctgtggtacc ggtgatgcca tactttgtta aatcgatctg tgccatcttg taattaacct
      661 ctttcctttg atagtaatac tgcttcattc tgcaaaatgc agaaatttga aggtatcctt
      721 tttaagaaac cggaccagcc ggcctcttct actctaatta tacataaaag gccaaaaaaa
      781 tcaactgaaa atcttccgtt tttttacaga acgtgtccca aaatctgtaa aaattttata
      841 aacttcgatt tttcgaaatt ttctgacaat tgcacttttg tacacttcat cctgcaaatt
      901 gacatttttt gattttttat gttataatat taacaattat gttcggaaag aagtttcttt
      961 atgtattacg atctgacccg cgggaaaatc tcccgctctc ttatcctgtt tgccctgccg
     1021 atgatcgcag gaaatctgtt gcagcagttt tacaacattg ccgacaccct gatcgtcgga
     1081 cgcgttctcg gaagcaatgc gctcgccgcc gtcggctcct cctatacgct gatgacgttt
     1141 ctgacttcca tttttctcgg cctgtcgatg ggcgccggtg cgctcttttc gatttatcag
     1201 ggacgaaaag atctttcttc tttaaaactt tcgattctcc acgcgtttct gctgatcctt
     1261 gccgttacgc tcgtgctgaa tctcgctgtt tatgcaggca tcgagccgat ccttacgttt
     1321 cttcgcgtcc cggatgaagt ccgtcccggc atgcggacgt atcttctgat tatctttgcc
     1381 ggccttattg caacatccgt atacaatttc ttttcctgta tgctgcgcgc gctcggaaac
     1441 tcttctgtgc cgctgatttt tctcgctgtc tctgctgtgc tgaacattgc actggatctg
     1501 ctctttgtcg ctgtactgcc gttcggcatc gcaggcgcgg cgctcgctac ggttcttgcg
     1561 cagtatgtgt ccgccgtcgg tattctgctc tatgtgctgc tccgctgccg catgtggctg
     1621 ccgtcaaaaa aagaatggcg tttcagccat tccattttaa aagagattct gaatctttct
     1681 tcgatgacct gcattcagca gtcggtcatg aatttcggca ttctgatggt acagggactc
     1741 gtcaacagct tcggtccggc ggtcatggcg gcatttgcgg ccgcggtgaa aattgatacc
     1801 tttgcctatc tgccggtgca ggatttcgga aatgcctact ccactttcac cgcacagaat
     1861 tacggggccg gaaatcacga gcggatccgg cgcggcacga aagatgcttt tgcactgagc
     1921 gctgcttttt ccatcgtcat ttctctgctt gtcaatctgt tcgcgcatcc gctgatgcag
     1981 attttcgtga aagcatcaga aatggaggtc atcgcgatcg gcgtacagta cctgcggctg
     2041 gaaggctcgt tctatctcgg catcggatgg ctgtttctgc tgtacgggta ttaccgcgct
     2101 gtcaaacgcg ccgagatgtc ggttgttctg accgtcattt cgcttggcat gcgtgtggcg
     2161 ctggcatacc ttttctccgc cacggcgctt ggcgttgccg gaatctggat cgccattccg
     2221 atcggctggg cactggctga tatgaccgga cttttcttcc tgcggcgtgc attccgatcg
     2281 cagaagcctg cataagttcc gaatcttata tcgcagtacg gttttctgcg gataaactgc
     2341 ggaactgccc cggcgtctgg tgcatgcgtt cctggaagac tcggttaaaa tagctgatat
     2401 ttcggaatcc gcagtcgctc gcaatcttgc tgatcgcctc ctcggtggaa agcagcagcc
     2461 ggcagctctc ggcaatccgc aggttattga catacgtcac ggcggaaata tgcataatcg
     2521 acttaaagaa gcggcagaaa gcgctctcac tcatatgaaa ctggctcgcc agttccgcga
     2581 tttcgagatc ctcacagaaa tgagcattca tgtatgtgag gatctctttc attctgctga
     2641 cacggtaatt cgtcgaggca tccgtttttc cgccgttctt ttctgccgca ctgtaatatc
     2701 cctgccagat tttcagaagt tccgctttta acagcagctc ccagcccggt tcttttcttt
     2761 cctgcagatt tcggatctcc tgcatggcct gtgccgtttt ttctgcaagg cgtgtccccc
     2821 gctctgtctt ggatggaaaa cggatctcgg aggacagcac tggttcgata tactgctgct
     2881 ggatccggtc aagcgatgtg ccggacagaa aacgcggctg gaatacaacg gcaagaaacg
     2941 taaattcctg tccgtcgatt ccctctacct catgcagacg gtttgcattg acaaaggcgg
     3001 tctccccttc ccgcaggaga aaggtctcgg tatccatatg aaaaacagct tcgcccttta
     3061 ctgcataaaa gagctccact tcctcatgcc agtggcagta cacccgcacg gtactctgca
     3121 ggtctgtctc gaaaacgtga atgctcagcg gaaaaagctc atcaccgtgg ggctgtgttt
     3181 cttttcggta attcatctgt gagagcatat ttttgtctcc tctcttgttt tgctctgctg
     3241 tcccaattgt aacacgaact ttgcgattcc ggcaagtttc catgttctgc cggcgaatcg
     3301 cccttccatg accgtatttt tccttcttaa atgataataa ttatcatatt ttatcagctt
     3361 attgacattt cttttcatcc gcgctatatt ttagataccc cgatagggta tgtgatttca
     3421 aatttttcat caaactccag gaatacagtc cggcacgctt tcgtttcgct gtattcccgg
     3481 aagaaagcag gtattgtatg attaaaacag tcttaaaaat tgatggtatg atgtgcggca
     3541 tgtgcgaatc ccatatgaat gatgtagtcc gcaagagctg ctctccaaag aaagtaaact
     3601 cttctgcaaa aaacggcgag accgttgtca tcagcgaagc accgctcgat attccgtatc
     3661 ttaaacagca gatcaaggac atcggatatg agctggtttc gtatacgagt gagccgtatg
     3721 agaaaaaggg atttttccat ttcggacgat aagcggattt catgcctttc cttttgcagt
     3781 ttgtgcgtcg gaaaaaagca ccgtttcaga agtttatttc cttttggaaa tctgcctctg
     3841 aaacggtgtt ttctattttt tatctatgat ttgatcagcc gcagcagcat ccgcaggacg
     3901 gcggcgtttc tttctcctct tttttcggag attctgcgcc cagatcgtac caggtcgggt
     3961 cgatgtcgaa tctgcggttg tttccaagat agatcggatg ggtcgggtct gaggacaggt
     4021 aggcgcgctc ggtcgaaacc ttccactgtg catcccagtc aatcttcgga agtgccagat
     4081 cgtagtgtgt gctggcgcgg aagccgtctt cctctccctc aattcttccc tggcggatga
     4141 tggctgcaaa atctgtgtga tgtgcaaatg ccggaatctg gtacagatct tttaagtaat
     4201 ccgaaacatt tttcagatcc tgcacacggt atttcaccgg tccgaggtaa cgggaatata
     4261 agaggtcaaa gcgcacaagg gtaacataaa gcgctgcgtc tgcctcggtc acaaagtctc
     4321 ctgccaggaa acgggtggct ccaagtgctg tttcgagctc ttcgagggct ccgtagaact
     4381 gttttgcact ttctacgtac tgtgccagtt ctacggcatt cagcatcgta tccagcggct
     4441 cggcgatctt tttctttact tcttcagcgg ctgctgtgga cgattttgca tttgctgttg
     4501 gtgacagctc cggtccgtcc ggcagttcgc ggatttcggc aagaatcgtc tgataatctt
     4561 cttctctagt atcagccagc gg
//
LOCUS       DAOMUF010000160         4574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5282_length_4574_cov_2.849967, whole genome shotgun sequence.
ACCESSION   DAOMUF010000160 DAOMUF010000000
VERSION     DAOMUF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5282_length_4574_cov_2.849967"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..339
                     /locus_tag="PLN77_08605"
     CDS             55..339
                     /locus_tag="PLN77_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HRM44363.1"
                     /translation="MAFAETFKALSDPVRRRILELLKNERLSAGEIGSHFDMTGATIS
                     YHLGILKKADLVLESREKNFIYYELNTSVLEEIMLWISELKGGTSDVEKI"
     gene            323..955
                     /locus_tag="PLN77_08610"
     CDS             323..955
                     /locus_tag="PLN77_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRM44364.1"
                     /translation="MWKKYKTTLILTTLISLFPMVIGLLLWNRMPDTIATHFGTNNVP
                     NGWSSKTMAVIGIPLFLAALQIFTAVLTFSDPKRQNIGPKMIRLVLWIIPVTSLIVCC
                     SIYANAVGITVDIGFVANGIVGLLFILIGNYMPKCKQNYTTGIKVPWTLHSQENWNRT
                     HRLAGWVWIIGGVAMIVNSFLQLEWILFLTIAALVLIPIGYSFLLFQKGI"
     gene            complement(1097..1951)
                     /gene="nadC"
                     /locus_tag="PLN77_08615"
     CDS             complement(1097..1951)
                     /gene="nadC"
                     /locus_tag="PLN77_08615"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRM44365.1"
                     /translation="MNPITRTLNVDNLLLLALQEDISSEDVTTNAVMPEACQGTVQLL
                     CKQDGVIAGMEVFARVFTLLDEKTEIIPYVKDGDEVKKGDLLATVNGDIRVLLSGERT
                     ALNYLQRMSGIATYTRSVAKLLEGSKTTLLDTRKTTPNMRIFEKYAVKIGGGQNHRYN
                     LSDGVLLKDNHIGAAGSVTKAIEMARDYAPFVRKIEVECENLDMVREAADAGADIIML
                     DNMTPAQMKEAVAVINGRAKTECSGNVTKENIATILDTGVDYVSSGALTHSAPILDVS
                     LKNLHPIE"
     gene            complement(1917..3293)
                     /locus_tag="PLN77_08620"
     CDS             complement(1917..3293)
                     /locus_tag="PLN77_08620"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRM44366.1"
                     /translation="MKKYYDAVIVGCGVAGCFAALHLPQEADILMITKADTEDSDSFL
                     AQGGICVLKNESDYDAFYEDTMHAGHYENTPESVDIMIRSSRNVINQLIAWGVDFRRD
                     ENGELCYTKEGAHSTNRILFHDDVTGKEITSKLLAQVKKLPNVTLLVHTEMCDILSCK
                     NVCEGVLIRDEEGKLIPVYARDVIWACGGIGGIYDNSTNFPHLTGDALAIAIHRNVAL
                     KNVNYVQIHPTTLYSQKKGRRFLISESVRGEGAVLLDKNGERFTDELQPRDVVSREIH
                     RQMEKDHTKHVWLSLKTIPLDVKERFPNIYQKCLEEGYDITKEPIPVVPAQHYFMGGV
                     KVDSNSETTMDHLYAAGETSCNGVHGANRLASNSLLESLVFAERAAKKMAAEWTAEGL
                     TENADRKETATEPKEDEWKLLAKRYDIRKEDYQNMEEYAECCKESIWDAIRKEDEYES
                     NHKNTKCG"
     gene            complement(3334..4242)
                     /gene="nadA"
                     /locus_tag="PLN77_08625"
     CDS             complement(3334..4242)
                     /gene="nadA"
                     /locus_tag="PLN77_08625"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRM44367.1"
                     /translation="MDTRREKLEALKKEKNAVIMAHYYVPDEVQEIADYIGDSYYLSD
                     MATKVDASVIVLCGVRFMGESAKILNPGKKVLLPDLHADCPMAHMAAIEKIEEVRKEY
                     PDVAVVCYVNSTAELKSHSDVCVTSSNAVKIVKELPNHDIFFIPDEHLGTYVAEQVPE
                     KHIILNDGFCHVHAAIRPEAAKAAKEARPQAKLLVHPECRSEVVALADYVGSTAGIIK
                     YAEASKDTEFLVATELGVFAELKKRCPDKKFYAVGNTQICPNMKKVTLDKLIEALENE
                     ENEVLLSEQEISDAHAPLLRMLELAK"
BASE COUNT         1039 a         1153 c          981 g         1401 t
ORIGIN      
        1 atttagatga atttctaatt aatttgcatt catactgccg gaaaggaggt ttccatggca
       61 ttcgcagaaa cgtttaaagc tctctccgat cctgtccggc gaaggattct ggagcttctg
      121 aaaaacgaaa gactttctgc cggggagatc ggatcgcact ttgatatgac aggtgcaacc
      181 atctcctacc atctcggtat tctgaaaaaa gcagaccttg tgctggaatc ccgggagaaa
      241 aattttattt attatgaact caacacttcc gtcctggaag aaatcatgct ctggatttca
      301 gagctcaaag gaggcacttc tgatgtggaa aaaatataaa acaacactga ttctgacaac
      361 actcatttct ctttttccga tggtgatcgg acttctgctc tggaatcgca tgccggacac
      421 cattgccacc cattttggca caaacaacgt tcccaacggc tggagcagca aaacgatggc
      481 agtgatcggt attcctctgt ttcttgccgc tctgcagatt tttaccgctg ttctcacttt
      541 ttctgatccg aagcggcaga atatcggccc gaagatgatc cgtcttgttc tgtggatcat
      601 acctgtaacc tccctgatag tctgctgctc catctatgca aacgcagtcg gcatcacagt
      661 tgatatcggt tttgttgcca atggaatcgt tggtcttctc tttattctca tcggcaatta
      721 catgccaaaa tgcaaacaga actacacaac cggcatcaag gttccatgga cccttcacag
      781 tcaggaaaac tggaaccgca ctcaccgcct cgccggctgg gtttggatca tcggcggcgt
      841 tgccatgatc gtaaacagct ttctgcagct tgaatggatt ttatttctca cgatcgcagc
      901 gctggttttg attccgatcg gatattcctt tctcctgttt cagaaaggaa tctaaacccg
      961 gagaattttt tataagaaaa ttcaacgaag tttcctgttt aaaaaagctt ctgttcttcc
     1021 ctttttccgg gaggaacaga agctttttta tttcgcgatt tttatatcta taaggaaatg
     1081 attcctattt ttatacttat tctattggat gcagattttt caatgataca tcaagaatcg
     1141 gtgcagaatg cgtcagtgca ccgctggaaa catagtctac gccggtgtcg aggatcgtgg
     1201 cgatattttc ttttgtcaca tttccggagc actcggtttt tgctctgccg ttgatgacgg
     1261 ctaccgcctc tttcatctgc gccggagtca tattgtcgag catgataatg tcggcacctg
     1321 catctgctgc ttcgcggacc atatcgaggt tttcacactc gacttcaatc ttacgcacaa
     1381 acggagcata atcgcgtgcc atctcgatcg cttttgtgac actgcctgca gctccaatat
     1441 ggttgtcctt taacagaacg ccatcggaca gattgtagcg gtgattctgg ccgccaccga
     1501 ttttgactgc atatttttca aaaatacgca tattcggggt cgtttttctg gtatcgagaa
     1561 gggtcgtttt ggaaccttcg agcagctttg caacgcttct cgtatacgtt gcaattccgc
     1621 tcatccgctg cagataattg agcgcggttc tctcaccgga cagcagaaca cggatatcgc
     1681 cgtttacggt tgcgagcaga tcgccttttt tgacttcatc gccgtctttc acatacggaa
     1741 taatctcggt tttctcatcg agcagagtaa atactcttgc aaatacttcc ataccggcaa
     1801 tgacgccgtc ctgtttgcag agaagctgta ccgttccctg gcaggcttct ggcatgacag
     1861 cattcgtggt tacatcttca ctggagatat cttcctgcag tgccaaaagc agcagattat
     1921 ccacatttag tgttcttgtg attggattca tattcatctt cctttctgat cgcatcccag
     1981 atgctctctt tgcagcattc tgcatattct tccatattct ggtaatcttc ttttctaata
     2041 tcgtaccgtt ttgcgaggag cttccattca tcttccttcg gctccgttgc cgtttctttg
     2101 cggtctgcat tctctgtaag accctctgcg gtccattctg cagccatctt ctttgctgca
     2161 cgttccgcaa atacgaggct ctccagcaga gaattgcttg ccagacggtt tgcgccgtgc
     2221 acgccgttgc agctcgtctc acctgctgca tacagatgat ccatcgtcgt ttcgctattg
     2281 ctgtctacct tgacgccacc cataaagtaa tgctgtgccg gtacaaccgg gatcggttct
     2341 tttgtgatat catatccctc ttccagacat ttctggtaaa tattcgggaa ccgttctttt
     2401 acatcaagcg gaatcgtttt cagggaaagc catacgtgct tcgtgtgatc cttttccatc
     2461 tggcggtgaa tctcacggga taccacgtca cgcggctgca gctcatcggt gaaacgctca
     2521 ccgtttttat cgagcaaaac agcgccctct ccgcggacag attcggaaat cagaaaacgg
     2581 cggcctttct tttgggaata cagcgttgtt ggatgaatct gtacgtaatt gacatttttc
     2641 agtgctacgt tcctgtggat tgcaattgca agtgcatctc cggtcagatg cgggaagttc
     2701 gtggaattat cgtaaattcc gccgatgcca ccgcatgccc agatcacatc cctggcataa
     2761 accggaatca gctttccttc ttcatcccgg atcagaacac cctcgcagac atttttgcag
     2821 gaaagaatat cacacatttc cgtgtgcaca agaagcgtca cgttcggcag cttttttacc
     2881 tgcgcgagaa gcttgctcgt aatctccttt ccggttacat cgtcgtggaa caagatgcga
     2941 ttggtagaat gtgcgccttc tttcgtatag cagagttccc cgttttcatc gcggcggaaa
     3001 tccacacccc aggcaatgag ctgattgata acatttcggg aagaacggat cataatgtcg
     3061 acggattccg gcgtattttc atagtgcccg gcatgcatcg tatcttcata gaacgcatcg
     3121 taatcgctct cgtttttcag aacacagata ccgccctgtg cgaggaagga gtcgctgtcc
     3181 tcggtatctg cttttgttat catcagaata tcggcttcct ggggcagatg aagtgccgca
     3241 aaacatcctg ccacaccgca tcctacaatc actgcatcat aatacttttt catttcattt
     3301 cctcttttcc gcttctgcgt tctgcttatt ttgttacttt gcaagctcga gcattcttaa
     3361 cagcggcgca tgtgcgtctg aaatttcctg ttcgcttaac agaacttcat tctcctcgtt
     3421 ttccagtgct tctattaatt tgtccaatgt cacttttttc atatttgggc agatctgcgt
     3481 atttcccact gcataaaact tcttatccgg gcagcgcttt ttcagctctg cgaaaacgcc
     3541 aagctcggtt gcaacgagaa actcggtatc tttacttgcc tcggcatatt tgatgatgcc
     3601 tgcggtgctt ccgacgtaat cggcaagtgc aacgacttca cttctgcact ccgggtgaac
     3661 gagaagcttt gcctgcggac gcgcttcctt tgcagccttt gcagcttccg gacggatggc
     3721 agcatgtacg tgacagaaac cgtcatttaa gatgatatgt ttctccggca cctgctcagc
     3781 cacataggta ccaaggtgct cgtcggggat aaagaagatg tcgtggttcg gcagttcttt
     3841 tacaattttt actgcattgg aggatgtgac acagacgtcg gaatgacttt ttaattccgc
     3901 ggtggaattg acatagcata cgacagccac atccgggtac tctttccgga cttcctcgat
     3961 tttttcaatc gctgccatgt gagccatcgg gcagtcggca tgcagatccg ggagcagtac
     4021 ttttttgccc gggtttaaga ttttggcgct ctcacccatg aaacgaacgc cgcagagaac
     4081 aatgacactg gcgtccactt tcgtcgccat gtcgcttaag taatacgaat ctccgatgta
     4141 atcggcaatt tcctgcactt catccggcac gtaatagtgt gccatgatga cggcgttttt
     4201 ttctttcttt aatgcttcca gcttttctct tctggtatcc attggttttg tttccttcct
     4261 atttttacta tcacgattca gaaccctgaa tcgtgatatt tttttaacac tttagcgctt
     4321 agtataccac aacttttgtc atgtgacaag acagttgtaa tatccattgt ccattttttt
     4381 ctttcctgcc tttcccttcc ctgtggtcta tcataggaag tacttgtcta agatataaaa
     4441 atctgtgacg cagtaattca cgttctgcag cgctgaagtt ttgcattatt ttctgggatt
     4501 gctataaaaa cacccggaag tttgacgggt caaagcggat attcgcaatc cgcttcgcta
     4561 cttgctcata actg
//
LOCUS       DAOMUF010000161         4545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5339_length_4545_cov_3.897996, whole genome shotgun sequence.
ACCESSION   DAOMUF010000161 DAOMUF010000000
VERSION     DAOMUF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5339_length_4545_cov_3.897996"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLN77_08630"
     CDS             complement(<1..309)
                     /locus_tag="PLN77_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRM44368.1"
                     /translation="MAEDFMAAAVKRVEEQFAKSGVSESCAAFERKDSQIEMRDGVKL
                     HTIYYFPREGGSGENKKYPVILTRTCYPGNDRIHRAYGEGLARRGYVYVYQYCRGREQ
                     S"
     gene            complement(314..613)
                     /locus_tag="PLN77_08635"
     CDS             complement(314..613)
                     /locus_tag="PLN77_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44369.1"
                     /translation="MKLKEIAHARTGDKGEISNISVIPYDENNYEMLKEKVTEARVAE
                     YFREICHGKVTRYELDGIHALNFVLDKTLGGGVTRSLALDKHGKTLGMALLEMEV"
     gene            complement(618..1970)
                     /locus_tag="PLN77_08640"
     CDS             complement(618..1970)
                     /locus_tag="PLN77_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HRM44370.1"
                     /translation="MKKIRIGSGAGYAGDRIEPAVELMEKGNLDYIIFECLAERTVAI
                     GQQDKEKDPSKGYNQLLDYRMEKILPLMAKNKVKVITNMGAANPVSAAERTCEIARKL
                     GVSGLKIACVTGDDITDELDKYENEKVLEDGTLLKEKKDVLISANVYMGAEGITKALE
                     EGANLVITGRVSDPALTIGPLVYEFGWNIDDNPDQMGQAVLAGHLLECAGQVTGGYFA
                     DPGYKDVPDLWKLGFPLIEIDETGAFTVTKVEGSGGLVSVDTCKEQMIYEIHNPKAYL
                     TPDATADFSKVTFRQIGENQVRAEHATTHGRPETLKVSVGYKDCFIGEGEISYGGSNC
                     MARAELAAEILEKRIELTGIELEEYRTDFIGCNSLYRDAISKEIGSGTPCEVRLRAAG
                     RTKDRKNAVLLANEVEALYTNGPAGGGGAVKRVSEIVSVCSIFVPRDAVEAKVTFREV
                     "
     gene            complement(2166..2648)
                     /locus_tag="PLN77_08645"
     CDS             complement(2166..2648)
                     /locus_tag="PLN77_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM44371.1"
                     /translation="MQVHSVLESLQQGILICDQHSRIVFFNQVYSDFIGVPLETAKGH
                     KITDYRKSAIAPEVIWSGIPVEGMVRREGTQEYFASVYPIREEHHIRGSISIVTSLVQ
                     FEKRESEAHMTLEERVRRFERQEIQNTLLLYGRDMEGKQKAAKELGISLATLYNKIKE
                     "
     gene            complement(2918..3292)
                     /gene="rplL"
                     /locus_tag="PLN77_08650"
     CDS             complement(2918..3292)
                     /gene="rplL"
                     /locus_tag="PLN77_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRM44372.1"
                     /translation="MAKLTTAEFIDAIKELSVLELNDLVKACEEEFGVSAAAGVVVAA
                     AAGDAAAAEEKDEFDVELTEVGPNKVKVIKVVREATGLGLKEAKEVVDGAPKVLKEGA
                     SKAEAEELKTKLEAEGAKVTLK"
     gene            complement(3364..3873)
                     /gene="rplJ"
                     /locus_tag="PLN77_08655"
     CDS             complement(3364..3873)
                     /gene="rplJ"
                     /locus_tag="PLN77_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRM44373.1"
                     /translation="MAINAKIDLKKPVLEEISAQVEGAQCVLLVNYSGLTVAQDTQLR
                     KELREAGVHYKVYKNTMMKRAFAGTAFESLTKDLDGTNAIAISKDDATAPARIIAKFA
                     KTAPKLEMVSGVVEGNYYDKAGMEALANVPSREELLGKLLGSIQSPIANLARVLNQIA
                     ESKGGAAEA"
     misc_feature    complement(3911..4055)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          983 a         1247 c          932 g         1383 t
ORIGIN      
        1 cgactgctct ctgccgcggc aatactggta gacatacaca tagcctctgc gcgccagtcc
       61 ctcaccgtag gcacggtgaa tacggtcgtt gcccggatag caggtacgag tcaggatgac
      121 gggatatttc ttgttttctc ctgatccgcc ttcccttgga aaataataga ttgtatgcag
      181 cttcacacca tcgcgcatct cgatctggct gtcttttctt tcaaacgctg cacagctttc
      241 ggaaacgccg cttttcgcaa actgttcctc cacgcgtttt accgcagccg ccataaaatc
      301 ttctgccatt gccctagacc tccatttcca gcaatgccat gccgagtgtc tttccatgtt
      361 tgtccagcgc cagcgatctt gtgacaccgc cgccgagtgt cttgtcgagg acaaagttca
      421 gcgcatggat tccgtccagt tcgtagcggg ttacttttcc gtggcagatt tcgcggaagt
      481 attctgctac gcgtgcttct gttacttttt ctttcagcat ttcataattg ttctcatcat
      541 atgggatcac cgaaatattg gagatctcgc ctttatcgcc tgttcttgca tgtgcaatct
      601 cttttaattt catcgcctta tacctcccgg aatgtaactt ttgcttctac tgcatcgcgc
      661 ggcacaaaaa ttgagcagac cgagacaatc tcggacaccc gtttcactgc tccgccgcct
      721 cctgcgggac cgttggtata gagagcctcc acctcgtttg ccagcagaac ggcatttttc
      781 cggtctttcg ttctgcctgc ggcgcggaga cgcacttcac acggagttcc acttccgatc
      841 tccttggaaa ttgcatcacg atacagggaa ttgcatccga taaaatcagt acggtactct
      901 tccagctcga tgcctgtcag ttcaatccgc ttctccagaa tctccgccgc cagctccgcc
      961 cgtgccatac agttgcttcc gccgtaggaa atttcaccct caccgatgaa gcagtccttg
     1021 tagccgacac tcactttcaa agtttccggt cttccatgcg ttgtcgcatg ctcggcgcga
     1081 acctggtttt ccccgatctg ccggaacgtc acctttgaaa agtccgctgt tgcatccggg
     1141 gtcaggtaag cctttgggtt gtggatttcg tagatcatct gctctttaca ggtatccaca
     1201 ctcaccagtc cgccacttcc ctcgactttt gtcacggtaa atgcgcctgt ctcgtcgatc
     1261 tcaatgagcg ggaatccaag tttccacaga tccggcacat ccttgtagcc cggatccgca
     1321 aaatagccgc ctgtcacctg tccggcacac tccagcagat ggcctgccag aaccgcctgt
     1381 cccatctgat ccgggttgtc gtcaatgttc catccaaatt catataccag cggtccgatc
     1441 gtcagtgcag gatcggaaac gcgaccggta atgacaagat tggcaccctc ttccaacgct
     1501 tttgtgatgc cctccgcacc catatagaca ttcgcagaaa tcagaacatc ttttttctct
     1561 tttaacagcg tgccatcctc cagcactttt tcattttcat acttatccag ttcgtctgta
     1621 atatcatcgc cggtcacaca ggcaatcttc aatccactga cacccagttt tctggcaatt
     1681 tcacacgtcc gttctgcagc agataccggg ttggcagctc ccatattggt aataactttt
     1741 actttgtttt tcgccatcag cgggagaatt ttttccattc ggtagtcgag cagctggttg
     1801 tatcccttcg acggatcttt ctctttgtcc tgctgcccga tcgcaacggt gcgttccgcc
     1861 agacactcga aaataatgta gtccagattt cctttttcca tcagctccac tgccggctca
     1921 atgcggtctc ccgcatagcc tgcgccgctt ccgattctga tttttttcat gttctatggc
     1981 ctcgctttct ttgatctgag atcattgtaa tgcgatatcc gggcagatac tacaaaaggg
     2041 atcgtaattt ctaaaatttt agaaatcatg atccctcttt tatgtgagtt ctgttacagc
     2101 aaagacagaa aataccacaa agccccaaag ctgcagaaat tccggcattt cattctcctt
     2161 ctgtcttatt ctttgatttt attatataac gttgccagcg aaattccgag ttcctttgcc
     2221 gctttttgct tcccttccat atcccgtccg tacagcaaca acgtattttg gatctcctgc
     2281 cgttcaaagc ggcgcacccg ctcttccagc gtcatgtgag cctctgattc ccgtttttca
     2341 aactgtacga gactcgtcac aattgagatg cttccgcgga tatgatgctc ttcccgaatc
     2401 ggataaacac ttgcaaagta ttcctgtgtt ccctctctcc gcaccattcc ctcgaccggg
     2461 atgccgctcc aaatgacttc cggagcaatg gcagatttgc ggtaatccgt gattttatga
     2521 cctttcgcgg tttcaagcgg aacgccgatg aaatcgctgt agacttgatt aaagaacaca
     2581 atcctgctgt gctgatcgca gatcaggatt ccctgctgta gagattccag aacggaatgt
     2641 acctgcattt ttcgttcctc ccgttttctc tttctgtact ctcggagtct ttctgtcatg
     2701 aggatattta gcccttatac tcctccgtaa atatcttgta tgaatagatc acattctcaa
     2761 ttttatctcc acggtcgcaa ttacattttg ataccacatg gcatcaccat accttccggc
     2821 aatttttaac tttattgcca caaagtatgg actcagttca aaaaacgcac ggcaggaaat
     2881 cccgccgtgc gtccatagat tctgatttac gcttaaatta tttaagagta actttagcgc
     2941 cttcagcctc aagtttagtt ttgagctctt cagcttctgc tttggaagca ccctctttga
     3001 ggactttcgg agcaccgtca actacttctt tagcttcttt caggccaagt cctgtagctt
     3061 cacgtacaac tttgataact ttaactttgt tcgggccaac ctctgtcagc tctacgtcga
     3121 actcgtcttt ctcctcagct gctgctgcat cgcctgctgc tgctgcaact acaacgcctg
     3181 ctgctgcgga tacgccgaac tcttcctcac atgcttttac aagatcgttt aactctaata
     3241 cagataactc tttgatagcg tcaataaact ctgcggttgt taactttgcc attttatttt
     3301 cctccttgga tattttctga ataatgttct caggctgcgt atttcacgct gcccgcttga
     3361 ggcttaggcc tctgctgcgc cgcctttgga ctctgcgatc tggttgagga cgcgagccag
     3421 gtttgcgatc ggggactgga tgcttccaag cagtttgcca agaagttctt ctcttgacgg
     3481 aacgtttgcc agtgcttcca ttccagcttt gtcgtagtag tttccttcta caacaccaga
     3541 aaccatctcc agcttcggag cggtttttgc aaatttagcg atgattcttg ccggagctgt
     3601 tgcatcgtcc ttgctgattg cgatagcgtt tgtgccatcc aggtctttgg taagagactc
     3661 gaaagcagta ccagcgaatg cacgtttcat cattgtattt ttgtatactt tgtaatgaac
     3721 gcctgcttct cttaactctt tacgcagctg agtatcctga gcaacggtta atccgctgta
     3781 gtttaccagc agcacgcact gtgcgccttc tacctgtgcg ctgatctctt ccaggaccgg
     3841 cttcttcaga tcgatttttg cattgattgc catgattgca cctccttgta gtatttcgta
     3901 cctacgcttt ggaagtataa aaaaagctct tcgcgaagat tggcgaagag atctcaaaat
     3961 tccataacat gaaccttgtg taacttctcc tcggcaggcg tttgaaactt acgccttgcg
     4021 gcacctgctg tcttcggcgt gatacaggtg gtatgatatc atatgtgagg cgggttgtca
     4081 agatcaaaat aaactttttt catatttgtg caacttcatc aaaatatgcg ttctattttt
     4141 tcaaaccgtt tcttcctata ccatgttata cttacgttgt tgtcgcctcc caaactggca
     4201 acaggaaggg ggtgagcgca tgtctatact tatttctctt atcgtttccg ttgtagcagg
     4261 tgtaatcagc tactatatct gcaagtggct agacggaaaa catggtgaca attagcccag
     4321 gcggatgccc ttccgtaaaa agggaagaac ccccagcagt tgccgctgct gggggttcgc
     4381 tttgtgaaca catgtcactt atttctctta tcagctacat atactatatc atatgcagaa
     4441 ccgaatttca agattcctct cccacatttt tatttcactt tcaccagtcg cacagccata
     4501 tacggtcttg acggcatgtc gatcgtgaat tctccgctgt ggcgg
//
LOCUS       DAOMUF010000162         4524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5372_length_4524_cov_3.851645, whole genome shotgun sequence.
ACCESSION   DAOMUF010000162 DAOMUF010000000
VERSION     DAOMUF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5372_length_4524_cov_3.851645"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            502..1863
                     /locus_tag="PLN77_08660"
     CDS             502..1863
                     /locus_tag="PLN77_08660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM44374.1"
                     /translation="MTKEGMGDEAMKKSLFYRAKKMPPSAQIMLLFAVEGVFLQYITS
                     INGFGLNLYATNMGATDSQIGIIQMVPNIVACAALLPLGILADRLKSTKTIPMLTLLV
                     MCAGYAFLGSVPALGERRMELFFVSLAFTAGALAIYNAQWQAMFGAAVSLRQRNDVYA
                     FRNQFMFVIGILAPVICGILMGRCHTADGKLLVLRSFFYLCAVCVLLQAAAIKKIPVE
                     QQEEGKAPVEAGKASSRFSVSDLLEAITSVGKNKALRWFFVPVVFFYITWQLDWSMWY
                     LGQVKYCKMSEMTLSICNGVFNIGQLAAVGMIAKVVRKKSPDFALIFAGIGLCFCPVI
                     MILVPHLPLAYRGVTFIVLMTVLNAPQCAVALCTVQILLNAAPEKNRSLLISLFTMMT
                     TLTNSVLPLLGVRLYTALGADLTALYRFNLIDLGVRILSTLGLVWRYQKAKKDGFLTK
                     ISD"
     gene            1976..2932
                     /gene="prmA"
                     /locus_tag="PLN77_08665"
     CDS             1976..2932
                     /gene="prmA"
                     /locus_tag="PLN77_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRM44375.1"
                     /translation="MKWNKFTLKTRSEVEDIVISTLADVGIEGVEIQDKQPLTESDKQ
                     QMFVDIMPDIPDDDGIAYLNFYLDVDEDKEKVLADVRAALAEMQEFLDLGECTITESE
                     TEDKDWINNWKQYFKQFYVDDILIIPSWEEVKPEDRDKMIIHIDPGTAFGTGMHETTQ
                     LCIRQLKKYVTKDTELLDVGTGSGILSIIALKLGARHAVGTDLDPCAVPAVEENKEVN
                     GIPVEAFDMMIGNIIDDKEVQDKVGYEKYDIVTANILADVLVPLTPVIVHQMKPGAVY
                     ITSGILDVKEEVVKEAVVAAGLEVVEVTHQGEWVSVTARKPM"
     gene            3007..3759
                     /locus_tag="PLN77_08670"
     CDS             3007..3759
                     /locus_tag="PLN77_08670"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRM44376.1"
                     /translation="MYRFFVEESQIYDGTVHITGSDVNHIKNVLRMRPGEKILVSTGG
                     EKEYHCAVSDFPEGEVLAAVEEVTAADRELPSGIVLFQGLPKGDKMELIIQKAVELGA
                     TEIVPVEMKRCVVKLDRKKAEKKAERWQTIALGAAKQSKRMQIPTVHMPVTFQQALAM
                     MAESDVRLMPYENAEGMEGTRRILESIEPGESIAILIGPEGGIDEAEVEMAMKEKVEP
                     ITLGKRILRTETAGMTVLSILVYLLEGRERTR"
     gene            3759..>4524
                     /locus_tag="PLN77_08675"
     CDS             3759..>4524
                     /locus_tag="PLN77_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRM44377.1"
                     /translation="MEAYLDNSATTCVYQETADLVCDLMVNHYGNPSAMHQKGVEAEQ
                     YIRTAQETLAKILKVKEKEIFFTSGGTESDNWALVGTAMANKRTGNHIITSAIEHPAV
                     SAPLAFLEEQGFRITRLPVNKEGLVDVNELEEAITPETILVSIMYVNNEIGAVEPIGE
                     IGRRIKAKNPKTYFHVDAIQAFGKYRIYPKRMGIDMLSVSGHKIHGPKGSGFLYIDEK
                     VKIRPLILGGGQQNGMRSGTDNVPGIAGLAKSAEIVY"
BASE COUNT         1257 a          952 c         1221 g         1094 t
ORIGIN      
        1 attctgagag caaaccccga aaataacgca aaaagcagtt ctgaattttt ttatcctctt
       61 ccggcaagaa gacacgttct gcggtgggag atgaatttat agcaagacca gaaaataacg
      121 caaaaaattc agcactgcag aacgtgaatt actgcgtcac ggattttcgt cgtaccccgg
      181 tacgccttca aatccgttcc ttgtaattcg cgccctgcag cactgccttt tttgcgttat
      241 tttctggtct tgctatagtt tctgagattg ctataaacat ttccaatagg cggagcccca
      301 ggtaaaacgg gggctccttt ttcttatgcc aaacccatgc attcaacagg gagaatgccc
      361 ttttttcttt tgtgaatatt gacagaaaat gccgttaaaa ttctcacatt ggatccgggt
      421 atttctgttg acatttgaac atgaaattgc cattatgaaa gcagaatctt tacgtatccg
      481 gcagggggca ggaacgtgaa gatgacaaaa gaaggcatgg gggatgaagc aatgaaaaag
      541 agtctgtttt accgggcaaa aaagatgccg ccatctgcgc agatcatgct gctgtttgcc
      601 gtcgagggtg tatttctgca atatatcaca tcgattaacg gtttcggtct gaatctgtat
      661 gcgacaaaca tgggtgcaac cgacagtcag atcggcatta tccagatggt gccgaacatt
      721 gtagcgtgtg cagcactgct gccgcttgga attctggcgg atcgtctgaa gtcgacaaag
      781 acgataccga tgcttacgct tcttgtgatg tgtgccggct atgcatttct tggaagtgta
      841 ccggcactcg gcgagagacg tatggagctg ttttttgtat cactggcatt tactgcgggg
      901 gcacttgcta tttacaatgc gcagtggcag gcaatgttcg gtgctgcggt ttcactgcgg
      961 cagcgaaacg atgtctatgc ctttcggaat cagttcatgt ttgtgattgg cattcttgca
     1021 ccggtgatct gcgggattct gatgggaaga tgccacacgg ccgatggaaa gcttctggta
     1081 ctccgaagct tcttttatct gtgtgctgtc tgtgttttgc tgcaggccgc tgcaatcaaa
     1141 aaaattccgg tggaacagca ggaggagggc aaagctccgg tagaagcggg gaaggcatcc
     1201 tcacgttttt ctgtttctga tctgctggag gcgatcacaa gcgtggggaa aaataaagca
     1261 ttgcgctggt ttttcgtgcc ggtggtcttt ttctatatca cctggcagct tgactggagc
     1321 atgtggtatt taggacaggt caaatattgc aaaatgtcgg agatgacact ttccatctgc
     1381 aacggtgttt tcaatatcgg tcagcttgcg gcagttggaa tgatcgcgaa ggttgtccgg
     1441 aaaaaatcgc cggattttgc actgattttc gccgggatcg gtctttgctt ctgcccggtt
     1501 attatgattt tggttccaca tttaccgttg gcgtaccgcg gggttacttt tattgttctg
     1561 atgacggttc tgaacgctcc acagtgtgcc gttgcactct gcacggtaca gattctttta
     1621 aatgccgcac cggaaaagaa ccgttcgctt ttgatcagcc tctttacgat gatgacgacg
     1681 ctgaccaaca gtgttctgcc gttgcttggt gtccgtctgt atacggcact tggggcagat
     1741 cttacggcac tgtaccggtt caatctgata gatcttgggg ttcgcatttt atccacactc
     1801 gggctggtat ggcgttatca gaaggcgaaa aaagatggct ttctgacaaa gatttcggat
     1861 tgagacgggc cgtaaaagcc gaaaataaca tttacaacag gaacgatatc gtgtatgata
     1921 aacgtggttt tctttgcacg tatggaaaag agaagcgcaa aaaggagttt aaacaatgaa
     1981 atggaataaa tttacattaa aaacaagatc ggaagtggaa gacattgtga tctccactct
     2041 ggccgatgtg ggaatcgaag gcgtggaaat tcaggacaaa cagcctttga ccgagagcga
     2101 caaacagcag atgttcgtag atatcatgcc ggatattccg gatgacgacg gcattgccta
     2161 cctgaatttt tacctcgatg tggatgagga caaggagaag gttcttgcag atgtccgcgc
     2221 ggcacttgca gaaatgcagg agttcctgga ccttggagag tgcaccatca cagagtctga
     2281 gacagaggac aaggactgga tcaacaactg gaaacagtat ttcaaacagt tctatgtgga
     2341 cgacattctg atcatcccgt cctgggagga agtaaagccg gaagaccggg ataaaatgat
     2401 catccatatc gatccgggca cagcctttgg aacgggtatg catgagacaa cacagctttg
     2461 catccgtcag ttaaagaaat atgtgacaaa ggataccgag ctgcttgatg tcggaaccgg
     2521 aagcggtatt ctttcgatta ttgcactgaa gcttggtgcg cgtcatgcgg tcggaacaga
     2581 tctcgatccg tgtgcggttc ctgcagttga ggaaaataaa gaagtcaatg gaattccggt
     2641 agaagcgttt gatatgatga tcggaaatat cattgatgac aaagaagtac aggacaaagt
     2701 cggatacgaa aaatacgata tcgttaccgc caatattctt gcagatgttc ttgttccgct
     2761 gacgccggta atcgtacacc agatgaagcc gggcgctgtt tatatcacat ccggcattct
     2821 cgatgtgaaa gaggaagttg tcaaagaggc agtcgttgca gcgggacttg aagtagttga
     2881 agttacacat cagggcgaat gggtttctgt tacagcaaga aaaccgatgt aaatcaggaa
     2941 aaacgaagta tcagaaaaac aagacatcag aaaagcgaaa taccagaaga gaaagagaac
     3001 agattcatgt accgtttttt tgtagaagaa agtcagattt atgacggcac tgtccacatc
     3061 acgggcagtg acgttaatca catcaaaaat gtgctccgca tgcggccggg cgaaaaaata
     3121 ctggtcagta ccggaggcga aaaggaatat cattgtgccg tttcggattt cccggaaggg
     3181 gaggttcttg ccgcagtaga agaagtcaca gcagcagaca gagaactccc gtccggaatt
     3241 gtgctgtttc agggtctgcc aaaaggtgac aaaatggaac tgatcatcca aaaagcagtg
     3301 gaacttggtg cgacggaaat tgttccggtg gagatgaaac gctgcgttgt gaagctggac
     3361 cggaaaaagg cggaaaagaa agcagagcgt tggcagacga tcgcgcttgg tgcggcaaag
     3421 cagtccaaac ggatgcagat tccgacggta cacatgcctg ttacatttca gcaggcgctt
     3481 gcaatgatgg cagaatcaga cgttcgcctg atgccgtatg agaacgcaga ggggatggaa
     3541 ggaacgagaa gaattctgga atccattgaa ccgggtgaat ctattgcgat cctgatcgga
     3601 ccggagggcg gcatcgacga agcggaagtg gaaatggcca tgaaggaaaa ggtagagccg
     3661 attacgctcg gaaagcgcat tctgcgcacg gaaacggccg gaatgaccgt gctttccatt
     3721 ctggtgtatc ttttagaagg aagggaacga acaagataat ggaagcatat ctggacaatt
     3781 ccgcaaccac ctgtgtttat caggaaaccg cagatctcgt ctgtgatctg atggtaaatc
     3841 attatggaaa tccgtctgcg atgcatcaaa aaggggtaga agcagaacaa tatatcagaa
     3901 ccgcccagga aacgctggca aagattctga aagtcaaaga aaaagaaatt ttctttacct
     3961 ccggtggcac agagtccgac aactgggcgc tcgtcggaac agcaatggca aataagagaa
     4021 cgggaaacca catcatcaca agcgcgatcg agcacccggc cgtgtcggca ccgctggcat
     4081 ttctggagga gcagggattc cgtattacgc gcctgccggt aaataaagaa ggccttgtgg
     4141 atgtgaacga actcgaagaa gcgatcacgc cggagacgat tctcgtttcg attatgtatg
     4201 tcaacaatga gatcggtgct gttgagccaa tcggggagat cggacgcaga atcaaggcga
     4261 aaaatccgaa aacgtatttt catgtcgatg cgatccaggc gttcggcaaa taccggattt
     4321 atccgaaacg gatgggcatc gacatgctct ccgtgagcgg ccacaagatc cacggaccga
     4381 agggaagcgg ctttttatat attgatgaga aagtaaaaat ccgtccgctg atcttaggcg
     4441 gcggccagca gaacggcatg cgttccggaa ccgacaatgt gccgggcatc gcaggccttg
     4501 cgaaatccgc tgagatcgtt taca
//
LOCUS       DAOMUF010000163         4517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5389_length_4517_cov_2.965038, whole genome shotgun sequence.
ACCESSION   DAOMUF010000163 DAOMUF010000000
VERSION     DAOMUF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5389_length_4517_cov_2.965038"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..517
                     /locus_tag="PLN77_08680"
     CDS             113..517
                     /locus_tag="PLN77_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HRM44378.1"
                     /translation="MLKQLSIFAENKKGTMQQITQILFENDVNILGSVNNDSAEYGIV
                     RMVVSDPDKAEQALQAAGYMTRQTDVLGVEVVDQPGNLNSLLKTLLETNIDIDYIYLS
                     FNRESGCPIMVFHVPDVYETEASLKSKGYTVL"
     gene            complement(595..1974)
                     /locus_tag="PLN77_08685"
     CDS             complement(595..1974)
                     /locus_tag="PLN77_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44379.1"
                     /translation="MRATTKEQGFYSQIFKLVLPIVIQNLLSAAVNSADVVMLNYVGQ
                     SAISAVSLASNYANVLFMVYYGLGTGATLLCAQYFGKGNMRAIQTVEGIALRFSVIIS
                     VVVAGIAFTMPQMMMRLFTDNAELIRIGASYLRVMGVTYLCWGITEIYLAVLRSLGRV
                     TISMAMNMMAFALNVFFNACFIFGLFGFPKLGATGVAIATAMSRVIELIGCVIVSIMS
                     KDIKLDLRYMFIRNKMLFSDFVKLSLPALGNDLSWGVAFSMYSVILGHLGSDAVAANS
                     LVVVVRNIGTVLCFGVASAGGILLGNVMGEGNLEKAKEYASKLLKLTVVTGAVGGLVV
                     LGITPFVLKFASLNETAMHYLKYMLLINTYYIMGTAVNTTLIAGVFRAGGDTKFGLVC
                     DTIDMWCYAVPLGFIAAFVFKLPVLVVYFLLCTDEFVKWPWVLKRYKSGLWAKNITRD
                     HLFEDEEKE"
     gene            complement(1997..3319)
                     /locus_tag="PLN77_08690"
     CDS             complement(1997..3319)
                     /locus_tag="PLN77_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRM44380.1"
                     /translation="MGEGHKKLNFSDCMGLAIGQIIGSGIMVLTGIVIGLTGHGIAAA
                     FILGAVLAVITCVPFIILTSAIPSSGAGFTYIRRLMGEKAGFMYIAMFVLSQVLIATY
                     AKGFASYFCSIFPQFGESAVAMAALVICTAINLIGLKSSALVQKGMVVLLLLSLFLFI
                     VFGLPKVSWDALTPTVSNLMPNGPKNFFTGVALLSFACGGAKFVAENGDDIVEPSRTI
                     PKVIVLSTSIVAVFYVLIGIVAGGVLPVETVAFQNLTLVAQEIFPTWLYLFFVFGGAV
                     FALLTTLNGTLSWVTRGLQAAAKQGWLPEKTAEENRNGVPAILLMVFFLMGAIPILTG
                     MDLTLISNMGVGTDMVTEFMVLLACWRLPDIFPEEYQKSAFCMKKRTLHILLFFIGIL
                     MIGTSYVNLSDLTVPAAIACGIYILVMFVYTQVRYPYVKAKQEKKEDK"
     gene            3620..>4517
                     /locus_tag="PLN77_08695"
     CDS             3620..>4517
                     /locus_tag="PLN77_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM44381.1"
                     /translation="MTNETFSDRLKLAMQAKQFKQVDLIRAAQRRGVKLGKSHVSQYV
                     SGKTVPRSDILHFLADVLQVDPDWLLAKDSTVNYTADTVSDPAESFSDPSAASLSQTK
                     TGGSPMREFKKSSKLDNVLYDVRGPVVEEANRMEACGTQVLKLNIGNPAPFGFRTPDE
                     VIYDMSHQLTDCEGYSPSQGLFSARKAIMQYAQLKKLPNVSIDDIYTGNGVSELINLC
                     MSALLDDGDEILIPSPDYPLWTACATLAGGKAVHYICDEQSEWYPDIDDMRKKITDRT
                     KAIVIINPNNPTGALYPKEVLQQ"
BASE COUNT         1257 a         1193 c          956 g         1111 t
ORIGIN      
        1 gtaaacgata cgctttttta cctttttttt cgtcttcttt tacaaaattc gttgacgcgt
       61 ttgattttgg cagtataatg gaatcagaga tcaattttag gaggccccca ttatgttaaa
      121 acaactttct attttcgcag aaaataaaaa aggtacgatg cagcagatca cacagattct
      181 gtttgaaaac gacgtcaata ttttaggttc cgtcaacaac gacagcgcag agtacggcat
      241 tgtccgcatg gtcgtttccg atccggataa agcagagcag gcgcttcagg ctgccggtta
      301 catgacacgc cagacggatg tactcggtgt cgaggtagtt gatcagccgg gtaacttaaa
      361 ttcccttctg aaaacactgc ttgagacaaa tatcgacatc gactatatct atctctcttt
      421 caaccgcgag tccggctgcc cgatcatggt gttccatgta ccggatgtgt acgagacaga
      481 agcaagctta aagagcaaag gatataccgt tctctagagc atgtttgaaa aacacgaaaa
      541 aagacatctg gcgaagtgat ttttcacctc tgcagatgtc tttttctata tcatctattc
      601 tttttcttca tcctcaaaca gatgatcccg cgtaatgttt tttgcccaca gtccgctctt
      661 ataccgtttc agaacccacg gccattttac aaactcgtct gtacacagca ggaaataaac
      721 cacaaggaca ggaagcttga agacaaacgc tgcaataaat ccaagcggca ccgcgtagca
      781 ccacatatca atcgtatcgc agaccagtcc gaactttgtg tcacctccgg cacggaatac
      841 tccggcaatc agcgttgtat tgacggcagt ccccataata taataggtgt taatcagcag
      901 catatatttc agataatgca tcgcggtctc atttaaagaa gcaaacttca gtacaaacgg
      961 cgtgataccc agtacaacca gtccgcccac agctccggta acaaccgtca gcttcagcag
     1021 tttagacgca tattccttcg ccttttccag atttccttct cccataacat ttccaagcag
     1081 aataccgccc gcacttgcca caccaaagca cagcacagtt ccgatatttc ttacaacgac
     1141 aacgagcgag tttgccgcta ccgcatcgct tcccagatgg cccaggatca cagaatacat
     1201 cgaaaatgca acgccccagg aaagatcatt tccaagcgcc ggaagtgaca gcttcacaaa
     1261 atcggaaaac agcatcttgt tgcggatgaa catgtaccgc agatcgagct tgatatcctt
     1321 actcatgatc gacacaatca cacagccgat cagttcaatg acacgcgaca tcgccgttgc
     1381 gattgcaaca ccggttgctc caagcttcgg aaatccgaag aggccaaaaa tgaagcatgc
     1441 gttgaaaaat acgttcagtg cgaatgccat catattcatc gccatgctga tcgtcacacg
     1501 gccgaggctt cgaagcaccg caagatagat ctccgtaatg ccccagcaca ggtaggtcac
     1561 acccatgacg cgcagatacg aagcaccgat ccggatcagt tccgcattat ctgtaaacag
     1621 acgcatcatc atctgtggca tggtaaaagc aattcctgcc acaacaacgg agatgatgac
     1681 ggagaatcga agtgcgattc cctcgacagt ctgaattgct cgcatattgc cttttccaaa
     1741 gtactgggca cacagaagtg ttgctcctgt tcccagtccg taataaacca taaataatac
     1801 atttgcatag ttggatgcca gggataccgc ggaaatagcg gactgtccca cgtagttcag
     1861 catcacgaca tcggcagagt tgactgccgc gcttaaaaga ttctgaatca caatcggcag
     1921 aaccagctta aaaatctggc tgtaaaagcc ctgctctttt gttgttgccc tcatgtttct
     1981 tctccatttc tcttctttat ttgtcttctt tcttttcctg ttttgccttc acatacggat
     2041 atcggacctg cgtgtaaaca aacatcacca gaatatagat gccgcatgcg atcgccgcgg
     2101 gaaccgtaag atccgacaga ttgacatacg aggttccgat catcagaatg ccgatgaaaa
     2161 acagcagaat gtgaagtgtc cgctttttca tacaaaacgc agacttctga tactcttccg
     2221 ggaagatatc cggcagacgc cagcaggcca ggagtaccat aaattccgtc accatatcgg
     2281 ttccgacacc catgttggaa atcaatgtca gatccattcc cgtaaggatc ggaatggcgc
     2341 ccatcaggaa aaagaccatc agaaggatcg ccggaacacc gtttctattc tcctccgcgg
     2401 ttttttccgg cagccagccc tgttttgcgg cagcctgcag tcctcttgtc acccagctca
     2461 aggttccgtt cagggtggta agcagcgcaa atacggcacc accgaacacg aaaaacaggt
     2521 acagccaggt tggaaaaatc tcctgtgcga caagcgtcag attctgaaat gcaaccgttt
     2581 cgaccggaag cacgccgcct gccacgatac cgatcagcac atagaataca gcaacgatac
     2641 tcgtggaaag cacgataacc tttggaattg tgcgggacgg ctccacaata tcgtctccgt
     2701 tctccgcaac gaattttgcg cctccgcacg caaaactcaa aagtgccacg cctgtgaaaa
     2761 agttcttcgg accgttcggc atcaggttgg ataccgtcgg tgtcagggca tcccagctta
     2821 ctttgggcag cccgaataca ataaataaga acaggctcaa aagcaacagc acaaccatgc
     2881 ccttttgcac cagtgcgctc gatttcagtc cgatgagatt gatggcggtg cagataacaa
     2941 gtgctgccat tgcgacagcc gactcgccaa actgcggaaa aatcgagcag aaatagcttg
     3001 caaagccttt cgcgtacgtt gcgatcagaa cctggcttaa gacaaacatc gcaatgtaca
     3061 taaatccggc tttttcaccc atcaatcgtc ggatgtaagt aaagccggcg cctgatgacg
     3121 ggattgcgga cgtcaggatg atgaacggta cacaggtgat aacggcaagc acggcaccta
     3181 aaataaacgc tgcggcaatg ccgtgtcctg tcagaccgat gacgattccc gtcagtacca
     3241 tgatgcccga accgatgatc tgtccgatcg caagtcccat acagtcggaa aaattcagtt
     3301 ttttatgacc ttcccccata cattttcctt cctttctctc ccgtttctat tggcggatcc
     3361 caacagaaac gcatagcatc ctattttttt attctaccga aatctcatcg tttgtcaatt
     3421 tgcaaaatgc gcaaggttta acgctttacc tgtacagaat agacaaatat tataattgaa
     3481 cttatttttc ttctattttg aacttttcaa tattttttac aaatttcagc ttttctattg
     3541 aactttcttc ccgctcatgc tacaataagc acagttttga actttcttta ttttgcattc
     3601 tattttaagg agattttcta tgacaaatga aaccttttcc gaccgtctga aactggccat
     3661 gcaggcgaaa cagttcaagc aggtcgattt gatccgagcg gcccagcggc gcggtgtgaa
     3721 gcttggaaaa agccatgtca gccagtatgt cagcggaaaa acggttccgc gctccgacat
     3781 tcttcacttt ctggccgatg tgctccaggt cgatcccgac tggctgcttg caaaagattc
     3841 cacagtaaac tatacggcag atacggtttc cgatccggca gaatcctttt ctgatccatc
     3901 tgccgcttcc ctttcccaga caaaaacagg aggttcccct atgcgagaat ttaaaaaatc
     3961 atcgaaactc gacaatgtct tatatgatgt ccgcggacct gtcgtagaag aagcaaaccg
     4021 catggaagcg tgcggcacac aggttctgaa actgaacatt ggaaatccgg ctccatttgg
     4081 cttccgtact cctgatgagg tcatctacga catgagccat cagcttaccg actgcgaggg
     4141 ttactctcct tcccagggtc tcttctctgc acgaaaagcg atcatgcagt atgcgcagtt
     4201 aaagaaactg ccaaatgttt ctatcgatga tatctacacc ggaaacggcg tcagtgagct
     4261 gatcaacctc tgcatgtccg ctcttctgga tgacggggat gaaatcctca tcccatcccc
     4321 ggattacccg ctctggaccg cctgcgcaac gctggccggt ggaaaagccg tccactacat
     4381 ctgcgatgaa cagtccgaat ggtatccgga catcgatgat atgcgaaaaa aaatcaccga
     4441 ccgcacaaaa gcgatcgtta ttatcaatcc gaataacccg accggtgcac tctatccgaa
     4501 agaggtactg cagcaga
//
LOCUS       DAOMUF010000164         4512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5401_length_4512_cov_4.282028, whole genome shotgun sequence.
ACCESSION   DAOMUF010000164 DAOMUF010000000
VERSION     DAOMUF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5401_length_4512_cov_4.282028"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1568
                     /locus_tag="PLN77_08700"
     CDS             180..1568
                     /locus_tag="PLN77_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRM44382.1"
                     /translation="MIVNLITTVYNFLWGDLFTIPLPGGGSLGISLLILLLIPTGIYF
                     TIRTRFLPIRLFPDMIRALSEKKNEKSSLSSFQTLIVSTATRVGMGNLVGVVAAVSAG
                     GAGAVFWMWVTAIIGSSTAFIEATLAQLHKEEDPLYGGYRGGPAYYIHDLVEDHLKKK
                     KRYVLPAVLFAIAGLICWCGISQVISNSVASSFKNAFSIPPLYTSIMLVILSAIIVLR
                     KNATVKVLDFIVPVMAVCYFFITLFIIAVNLRQIPSVFARIFEEAFGLRQMAAGGFGA
                     VLMNGVKRGLFSNEAGSGSAPCAAAAAECDRPAKAGLVQALGVFVDTIVICSCTAMIM
                     LLAPKDLINGLSGMDLLQTAMQYHLGKFGVIFIALTLFLFSFSTFLGILFYARSNVAY
                     LFGDKWCFQTAYKVLALVMLFIGGIAAYTFVWDLGDVGIGLMTIFNIIALYPLAGEAL
                     SELKSYEESKKS"
     gene            1629..2534
                     /locus_tag="PLN77_08705"
     CDS             1629..2534
                     /locus_tag="PLN77_08705"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM44383.1"
                     /translation="MIIEPSNYTYETMPDYTDAVDGAVEIPVTGEEMEIRYAHEVVYD
                     EAHNLHLEIFTPFQMAHPERTWPCITFIQGSAWMKQYVYQKVGMIARLAQRGYVVAIV
                     EYRHSGIAHFPAQIIDAKNAVRFLRAHAEEYKLNPSQMFLMGDSSGGQVSSVAGMTAK
                     SGKLDEPINEESCEVCGIIDLYGAVDVTLPYGYPITLNHQKPDSPEGMLMGYDITEHL
                     EEAKAACSKTYVNEDFPPMLIAHGTKDTMVFCQQSVDLYHALKDAGKDVQLYLLRGAD
                     HGGAAFWTEEMITVYDNFFKKCLGE"
     gene            2536..3276
                     /locus_tag="PLN77_08710"
     CDS             2536..3276
                     /locus_tag="PLN77_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM44384.1"
                     /translation="MRIAYCEDEPAQRSRMELLLRTFAERKRLPLTVEAFSDSAAFLF
                     QYPGSYPFDLIFLDIDLGGMDGMALARKIRETDSEVPILFLTNHKEYVFEGYEVQAFR
                     YLLKPVTEDTLFPLLEELQAMRGQEKPHLLLSLGGETQKLFLSDLLFVESDNHYLTLH
                     TAAGTETFKKNLSEFREELSAAWQASGGTGECFAAAHRSYLVNLAHVERITRTECLLS
                     DGTKIPVSRGCYASLNHAFLQFYRPNAF"
     gene            3328..3987
                     /locus_tag="PLN77_08715"
     CDS             3328..3987
                     /locus_tag="PLN77_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRM44385.1"
                     /translation="MAHIPFLSHYLERKNIEYQNQLLARQVEEVQNIYLTMRGWRHDY
                     HNHLQSMKAYVEMGQYDKLQEYLGLLENDLDQVNQLCDSGNVNLDAILNSKISLAEKN
                     GIRVDYKAACPANLTVSDIDLCALIGNLIDNAVEACEGLADPYIRLYIGVLKNQLYIS
                     VTNATKDTVRKLDSEYITTKRGNHGHGLKRINLVVEKYEGYINRQNEPGVFVTEIMLP
                     L"
     gene            4172..>4512
                     /locus_tag="PLN77_08720"
     CDS             4172..>4512
                     /locus_tag="PLN77_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44386.1"
                     /translation="MYSLWSNYIYIHRELWHYSKKPFLFQALEIVSQVLTGFFGILLP
                     AGILLFLEQSSGFSGFALGTLVLFLCYAVVFVSHTFLQSRNAWQYTEFRTGFFTRRLL
                     CHFMQIDYAVLT"
BASE COUNT         1046 a         1096 c         1134 g         1236 t
ORIGIN      
        1 ttttaagagg cagatatcaa ctttttctgg tttcggaaaa agctgatatt tgcctcttat
       61 cttttctctt cccttcttta cattccaaac agaaaccgat ataatagcag gtaacggtgc
      121 ttttatgctt tgacgtttca cagtgccgca aatattttga aatgagggtt tttgaaaaca
      181 tgattgtcaa tctcatcacc acagtctaca actttctctg gggcgatctt tttacgattc
      241 cgctcccggg cggcggcagt ctcggaatct cccttttgat tcttctgctg atcccaaccg
      301 gcatctattt tacgatccgg acacgttttc ttcctatccg tctgttcccg gacatgatcc
      361 gtgccctgtc ggaaaagaaa aatgaaaaaa gcagcctttc ttcttttcag acactgatcg
      421 tttccaccgc aacgcgtgtc ggtatgggaa atctggtcgg cgttgtcgct gcagtctccg
      481 ccggaggcgc aggtgctgtg ttctggatgt gggtgacggc tatcatcggc tcgtctaccg
      541 cgtttatcga agctacgctc gcacagcttc acaaagaaga agatcctctg tacggcggct
      601 accgcggcgg tcctgcctac tacattcacg atctggttga agaccacctc aagaagaaaa
      661 aacgatacgt tctgccggct gttctgtttg ccattgccgg tctgatctgc tggtgcggta
      721 tcagtcaggt aatcagcaac tccgtggctt cttcttttaa aaatgcattt tccattccgc
      781 cgctttacac cagtatcatg ctggtgattc tttctgcaat catcgtactg cggaaaaatg
      841 caaccgtaaa ggtgctggat tttatagttc ctgtcatggc agtctgctat ttctttatca
      901 cactgtttat catcgcagtc aacctccgtc agattccttc tgtgtttgca cgcatttttg
      961 aggaagcctt cggacttcgt cagatggcag ccggcggttt tggtgctgtt ctgatgaacg
     1021 gtgtaaagcg tggtctgttt tcgaatgaag ccggttccgg ctccgctcca tgcgccgctg
     1081 ccgccgcaga atgtgaccgc ccggccaaag caggccttgt acaggcgctc ggcgtttttg
     1141 ttgacacaat cgtcatctgc agctgcaccg caatgattat gctgcttgca ccaaaggatc
     1201 tgattaatgg cctttccggc atggatctct tgcagaccgc catgcagtat catctgggaa
     1261 aattcggtgt gatttttatc gcacttacgc tgtttttgtt cagcttttcc acgttcctcg
     1321 gcattttgtt ctatgcaaga agcaacgttg cctacctgtt cggtgacaag tggtgtttcc
     1381 agaccgctta caaggttctt gcacttgtaa tgctgtttat cggcggtatt gccgcataca
     1441 cttttgtgtg ggatcttggt gatgtcggca tcgggctgat gacaattttc aacattattg
     1501 cactgtatcc acttgcaggt gaggcgcttt ccgagttaaa atcatatgag gagtcgaaga
     1561 aatcgtaaat cctgtggatt gctgtttcgg accgaaaaag gggtttttga aaaaggaggt
     1621 ttttacacat gatcattgaa ccatcaaact acacgtatga gacaatgccg gactacacag
     1681 acgcggtaga cggcgccgtg gaaattccgg tgaccggaga ggaaatggag atccgctatg
     1741 cgcatgaagt tgtctacgac gaggcgcaca atcttcatct ggaaattttc acaccattcc
     1801 agatggcgca cccggagcgc acctggccgt gcatcacatt tattcaggga tccgcctgga
     1861 tgaaacagta tgtctaccag aaggtcggca tgatcgcccg tctggcgcag cgcggctacg
     1921 ttgttgcaat cgtcgaatac cgtcacagcg gaatcgcaca tttcccagcg cagatcatcg
     1981 acgcaaaaaa tgcggtacgt tttctgcgtg cgcatgcgga ggaatacaag ctgaatccat
     2041 cgcagatgtt cctgatggga gattcttccg gcggacaggt ttcttccgtt gccggcatga
     2101 ccgcaaagag cggaaaactt gacgagccga tcaatgagga aagctgcgag gtatgcggta
     2161 ttatcgatct gtacggagct gttgatgtga cactgccgta cggctatccg ataacgctga
     2221 accatcagaa accagacagc ccggagggca tgctgatggg ctatgacatt accgagcatc
     2281 ttgaggaggc taaggcagcc tgctccaaaa catacgtgaa cgaggatttc ccgccgatgc
     2341 tgatcgcaca cggcaccaag gacacgatgg tgttctgcca gcagagtgtc gatctctacc
     2401 acgcattaaa ggatgccgga aaagatgtac agctgtatct tcttcgcggc gcagaccacg
     2461 gcggtgcagc attctggacc gaggaaatga ttaccgttta cgataacttc ttcaaaaaat
     2521 gtttgggtga ataacatgag aattgcttac tgcgaagatg aaccggcgca gcgttcccgg
     2581 atggaactgc tgctccgcac ctttgcggag cgaaaaaggc ttcctctgac cgtggaagcc
     2641 ttttcagact ccgccgcttt tctgttccag tatcccggct cgtatccgtt tgatctcatc
     2701 tttctcgata ttgatctcgg cggaatggac ggcatggcgc tggcgcggaa aatccgtgaa
     2761 acggacagtg aggtgccgat tctctttctg acgaatcata aagaatatgt tttcgaagga
     2821 tatgaggtgc aggcgttccg gtatctctta aagccggtga cagaggatac actgtttccg
     2881 ctgctcgagg agctgcaggc gatgcgcgga caggaaaagc cgcatctgct gctttcgctt
     2941 ggcggggaga cgcagaagct gttcctctcg gatctgcttt ttgtggaatc cgataatcat
     3001 tatctgacgc tgcatacggc cgccggcacg gaaacgttta agaaaaatct gtcggagttc
     3061 cgcgaagagc tttccgccgc atggcaggct tctggtggga caggagaatg ttttgcggcc
     3121 gcacaccgga gttatcttgt gaatctcgct catgtggagc ggatcacgcg gacggaatgt
     3181 ctcttgtcgg acggaacaaa aattccggtc agccgcggat gctatgcatc actcaaccat
     3241 gcgttcctgc agttttaccg gccgaatgcg ttttaaaggt tttgcatttt ttaatttcca
     3301 attccgaacg aaccgaggtg atgtttcatg gcgcacattc catttttatc ccattatctg
     3361 gaacgcaaaa atatcgaata ccagaaccag cttctcgccc gccaggtcga ggaagtgcag
     3421 aatatctatc tgacaatgcg cggctggcgc catgattacc acaatcatct gcagtcaatg
     3481 aaggcatatg ttgagatggg acagtatgat aagctgcagg aatatctcgg gctgctcgaa
     3541 aatgatctcg atcaggtcaa ccagctctgc gactcgggga atgtgaatct cgatgcgatt
     3601 ttaaattcca aaatttcact cgccgaaaag aacggcatcc gcgtggatta caaggcagcc
     3661 tgcccggcca atcttacggt gagtgatatt gatctctgcg cgctgatcgg aaatctgatt
     3721 gacaatgcgg tggaagcatg cgagggcctt gcggatccct atatccggct ttatattggt
     3781 gtgctgaaaa accagctgta tatttccgtt acgaatgcaa caaaggatac tgtgcggaag
     3841 ctggacagcg agtatatcac cacgaagcgc ggaaatcacg ggcatgggct gaaacggatc
     3901 aatcttgttg tggaaaagta cgaaggatat atcaaccggc agaacgagcc cggcgttttc
     3961 gtgacggaga ttatgctgcc gctgtaaaaa gctctttcca gaattttttg attttgtccg
     4021 atgattctac agaaaaattt tgcagaacgt gaacactttt tttgctgttc gcgttctttt
     4081 ttgttccatt tacgtacttt tcccccgtgt tttgcatgaa tatggtagga taactacagt
     4141 tcacgaaaga catgtgacag aggaggtctt tatgtattca ttatggtcta actacattta
     4201 tattcatcgg gagctgtggc attacagcaa aaagccgttt ctctttcagg cactcgaaat
     4261 cgtctcacag gtgctcaccg gctttttcgg gattctgctt cctgccggga ttctcctgtt
     4321 tctggaacag agcagcggct tttccggatt tgcacttgga acgctcgttc tctttctttg
     4381 ctatgccgtt gtttttgtca gccacacctt tctgcaatcg cggaacgcct ggcagtatac
     4441 cgaattccgg accggctttt ttacgcgccg ccttctctgt catttcatgc agatagatta
     4501 tgcggttctg ac
//
LOCUS       DAOMUF010000165         4461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5483_length_4461_cov_5.415570, whole genome shotgun sequence.
ACCESSION   DAOMUF010000165 DAOMUF010000000
VERSION     DAOMUF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5483_length_4461_cov_5.415570"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2179)
                     /gene="rpoC"
                     /locus_tag="PLN77_08725"
     CDS             complement(<1..2179)
                     /gene="rpoC"
                     /locus_tag="PLN77_08725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRM44387.1"
                     /translation="MPETAKKENYQMSFDAIKIGLASPEKIREWSHGEVKKPETINYR
                     TLKPEKDGLFCERIFGPSKDWECHCGKYKKIRYKGVICDRCGVEVTKSSVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLILDLSPRTLEKVLYFANYIVLDAGDTKLQYKQVLTEK
                     EYQDAREEFGNSFRAGMGAESIKELLMAIDLEKESAELKAGLKDSTGQKRARIIKRLE
                     VVEAFRESGNRPEWMILDAVPVIPPDLRPMVQLDGGRFATSDLNDLYRRIINRNNRLK
                     RLLELGAPDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKSGRF
                     RQNLLGKRVDYSGRSVIVVGPELKLYQCGLPKEMAIELFKPFVMKELVSNGTAHNIKN
                     AKKMVEKLEPQVWDVLEDVIKEHPVMLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVC
                     TAFNADFDGDQMAVHLPLSVEAQAECRFMLLSPNNLLKPSDGGPVAVPSQDMVLGIYY
                     LTQERPGEKGEGSFFRDMNEAILAYENGYITLQTKITIRCEKEMEDGTVMQQNVSSTL
                     GRFLFNEILPQDLGYVDRTVPGNELALEVDFLVAKKQLKQILEKVINTHGATKTAEVL
                     DYIKATGYKYSTRAAMTVSISDMTVPPQKPEMLAEAQATVDKITRNFKRGLITDEERY
                     KEVVDTWKKTDDALTKALLDGLDKYNNIFMMADSGARGSDKQIK"
     gene            complement(2218..>4461)
                     /locus_tag="PLN77_08730"
     CDS             complement(2218..>4461)
                     /locus_tag="PLN77_08730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRM44388.1"
                     /translation="SHYGRMCPIETPEGPNIGLINSLASYARINEYGFVEAPYRRIDK
                     SDPKNPRVTDEVVYMTADEEDNYHVAQANEPLDAEGHFLNKNVSGRYLDETQSYEPHM
                     FDYMDVSPKMVFSVATALIPFLQNDDANRALMGSNMQRQAVPLMITDAPVVGTGIEAK
                     AAVDSGVCVVAKKAGTISYSCSNEIRMNNDDGTKDVYHLTKFMRSNQSNCYNQKPIVF
                     KGDHVEAGEVIADGPSTYQGELALGKNPLIGFMTWEGYNYEDAVLLSERLVQDDVYTS
                     IHIEEYEAEARDTKLGPEEITKDVPGVGDEALKDLDERGIIRIGAEVRAGDILVGKVT
                     PKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGEYGIIVDAKVFTRENGDELSPGV
                     NQAVRIYIAQKRKISVGDKMAGRHGNKGVVSRVLPVEDMPFLPNGRPLDIVLNPLGVP
                     SRMNIGQVLEIHLSLAAAALGFNVSTPIFDGANEKDIQDTLELANDYVNMEWDAFSEK
                     YKDILLPEVYQYLEEHLDHRELWKGVDIARDGKVQLRDGRTGEPFDGRTTIGHMHYLK
                     LHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASYTLQEILT
                     VKSDDVVGRVKTYEAIIKGDNIPEPGIPESFKVLLKELQSLALDVRVLRDDNTEVKIM
                     ESVDYGDTNLNHIIEGDRYSDREDERFGDYGFSKKEFDGEEMVDVEEDEEEDSYLDLD
                     ESSDDDM"
BASE COUNT          967 a         1191 c         1064 g         1239 t
ORIGIN      
        1 gtttgatctg cttatcggaa ccacgggctc cggagtcagc catcatgaag atgttattgt
       61 atttatccag tccgtcgagc agtgcttttg tcagcgcatc gtcggtcttc ttccaggtat
      121 ctacaacctc tttgtaacgc tcttcgtctg taatcagacc acgtttgaag tttcttgtaa
      181 tcttgtctac cgttgcctgt gcttcggcga gcatctccgg cttctgcggc ggcacggtca
      241 tatcggaaat ggatacggtc atggctgcac gggtggagta tttatatccg gttgccttga
      301 tatagtccag aacctctgcg gttttggtag ctccgtgtgt attgataact ttttccagaa
      361 tctgtttcag ctgtttcttt gcaacgagga aatcaacctc aagcgcaagc tcgttacccg
      421 gaaccgtacg gtctacgtat cccagatcct gcggcagaat ctcattgaag aggaatcttc
      481 cgagtgtgga gcttacgttc tgctgcatta cagtaccatc ttccatctct ttttcgcagc
      541 ggatggtaat cttggtctgc agggtaatgt atccgttctc gtatgccagg atggcttcgt
      601 tcatatcgcg gaagaagctt ccttcgccct tctcgcccgg acgctcctgt gtcagatagt
      661 agataccaag aaccatatcc tgggaaggaa cggctaccgg accaccatcg gacggtttca
      721 gcaggttgtt cggagaaaga agcatgaaac ggcactctgc ctgcgcttct acagacagcg
      781 gcagatggac agccatctgg tcaccgtcga agtcggcgtt gaatgcggta caaaccagcg
      841 ggtgcagctt aatcgcttta ccttctacca gaatcggctc gaacgcctgg atacccagac
      901 gatgcagcgt aggggcacgg ttcagcataa ccggatgctc tttgatgacg tcctcgagaa
      961 cgtcccatac ctgcggctcg agtttctcga ccattttctt tgcatttttg atgttatgtg
     1021 cagtgccgtt ggaaaccagc tctttcataa cgaacggttt aaacagctcg attgccattt
     1081 ctttcggcag accgcactgg tacagtttca gttcaggacc aacaacgata acggaacggc
     1141 cggagtagtc aacacgttta ccaagaaggt tctgacggaa acggccggat ttacctttca
     1201 gcatgtcgga cagagatttc agaggacggt ttccaggacc ggttaccgga cggccgcggc
     1261 ggccgttgtc gatcagggca tcaactgcct cctggagcat acgtttctcg ttgcgcacga
     1321 tgatatccgg cgcaccgagc tccagcagtc tcttcagacg gttgttacgg ttgatgatac
     1381 ggcggtacag gtcattcagg tcggatgttg caaaacgtcc tccgtcaagc tgtaccatag
     1441 gacgcagatc cggcggaata accggaaccg catcaagaat catccactcc ggacggtttc
     1501 cggactcacg gaatgcctca acaacctcaa gtctcttgat gatgcgggct ctcttctgtc
     1561 cggtagaatc tttaagacct gcctttaatt cagcagactc tttttccaga tcgattgcca
     1621 tcagaagctc tttgatggat tccgcaccca tacctgcacg gaagctgttt ccgaactcct
     1681 ctctggcatc ctggtattct ttctcggtca gaacctgttt gtactgtaat ttggtatcgc
     1741 ctgcatcaag aacgatgtag tttgcgaaat acagaacctt ttccagagtt ctcggagaca
     1801 gatccaggat cagtcccata cgggacggaa ttcctttgaa ataccagatg tgggataccg
     1861 gagctgccag ctcaatgtgt cccatacgct cacggcgcac actggatttt gttacttcta
     1921 cgccgcatcg gtcgcagatt acgcctttgt agcgaatttt cttatattta ccgcaatggc
     1981 actcccagtc tttgcttggt ccgaagattc tttcacagaa cagaccgtct ttttccggtt
     2041 tcagcgttct gtagttgatg gtctccggtt tttttacctc gccatgggac cactcccgga
     2101 ttttctccgg ggaggcaagt cctattttga tggcatcaaa gctcatctgg taattttctt
     2161 tttttgctgt ttctggcatt tgattacccc tttcctcgca aagcgaagat ctacaagtta
     2221 catgtcatca tctgaacttt catccagatc aagataagag tcttcttctt catcctcttc
     2281 cacatctacc atttcttctc cgtcaaactc tttcttggag aatccatagt caccaaatct
     2341 ctcgtcctct ctgtcagagt aacggtctcc ctcgatgatg tggttcagat tggtatcacc
     2401 gtaatcaact gattccataa ttttaacttc ggtattgtca tcacgcagta cgcggacatc
     2461 gagtgccagg gactgcagct ctttcaacaa tactttgaag gattccggaa taccaggctc
     2521 cggaatattg tcgcccttga tgatggcttc gtatgtcttc acacggccaa ccacatcgtc
     2581 agacttcaca gtcaggatct cctgcagcgt gtaggatgca ccgtacgcct caagagccca
     2641 aacctccatc tctccgaaac gctgtccacc gaactgagct ttaccaccca gcggctgctg
     2701 tgtaacgagg gagtatgggc cggtagaacg tgcatggatc ttatcgtcta ccaggtgatg
     2761 cagtttcagg taatgcatgt gtccgatggt ggttcttcca tcaaacggct caccggtacg
     2821 tccgtcgcgg agctgtacct taccgtctct tgcgatatca acacccttcc acagttctct
     2881 gtgatccaga tgctcttcca gatactgata tacttccgga agcagaatat ctttatattt
     2941 ctcggagaat gcatcccatt ccatgtttac ataatcgttt gccagttcca gggtatcctg
     3001 aatatctttc tcgtttgcgc cgtcgaaaat cggggtggat acgttaaatc caagcgctgc
     3061 tgctgcaagg ctcaggtgga tctccagcac ctgtccgatg ttcatacggg acggcacacc
     3121 cagagggttc agcacgatgt ccagcggacg tccgttcggc aggaacggca tatcctctac
     3181 aggaagcaca cgggaaacga cacccttgtt tccgtgacga ccagccatct tatcgccgac
     3241 agagattttt ctcttctgtg cgatgtagat gcggactgcc tggtttactc caggagaaag
     3301 ttcgtcgccg ttttcacggg taaatacttt ggcgtccacg atgataccat actcaccgtg
     3361 cggtactttc agggaagtat cacgtacttc tcttgctttc tcgccaaaga tggcacggag
     3421 cagacgctcc tctgcggtca gctcagtctc tcccttcgga gttacttttc cgaccagaat
     3481 atcaccggca cgtacctctg caccgatgcg gatgattcca cgctcgtcca ggtcttttaa
     3541 ggcttcatcg ccgacgcccg gaacgtcttt tgtgatctct tccggtccaa gcttggtgtc
     3601 acgggcctct gcctcgtact cctcgatgtg gatggaagtg taaacatcat cctgtaccag
     3661 acgctcgcta agaagaacgg cgtcctcgta gttatatcct tcccaggtca taaatccgat
     3721 cagcgggttt ttaccaagtg caagctcgcc ctggtaagta gacggaccgt ctgcgattac
     3781 ttcgccggcc tctacatggt cacctttaaa tacgatcggt ttctggttgt agcagttgct
     3841 ctggttgctt cgcatgaatt tggtcagatg gtaaacatct tttgttccgt catcattgtt
     3901 catgcggatc tcgttggagc aggagtagga aatggttcct gctttctttg caacgacgca
     3961 gacaccggag tctacggctg cttttgcttc gataccggtt cctactacag gagcatcggt
     4021 gatcatcagc ggtacggcct gacgctgcat gttggatccc atcagcgcac ggttcgcatc
     4081 gtcgttctga aggaacggga tcagcgccgt agcaacggag aataccatct tcggagacac
     4141 gtccatgtaa tcaaacatgt gcggctcgta ggactgggtc tcatccagat aacgtccgga
     4201 aacgttcttg ttgaggaagt gtccttctgc atccagcggc tcatttgcct gtgctacatg
     4261 gtaattatcc tcttcatctg cggtcatgta gactacttcg tctgtaacac gcggattttt
     4321 cggatctgat ttgtcaattc ttctgtatgg agcctcaacg aagccatact cgttgatgcg
     4381 cgcatacgat gccagagagt tgataagacc gatgttcggt ccctcaggag tctcgatcgg
     4441 gcacattctt ccgtaatggg a
//
LOCUS       DAOMUF010000166         4459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5485_length_4459_cov_4.006585, whole genome shotgun sequence.
ACCESSION   DAOMUF010000166 DAOMUF010000000
VERSION     DAOMUF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5485_length_4459_cov_4.006585"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLN77_08735"
     CDS             complement(<1..244)
                     /locus_tag="PLN77_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44389.1"
                     /translation="MRLRNKDEKIKMKNTKRRGCAQCTASSFTYTTENCLRQPSLKGE
                     TAVGRILLSQGNMGFSSRFFSLPTGYYTQFFAICTRY"
     gene            complement(228..1517)
                     /locus_tag="PLN77_08740"
     CDS             complement(228..1517)
                     /locus_tag="PLN77_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HRM44390.1"
                     /translation="MKETLPKSIQELILSLREKLKDEPEIVDMFERCYSNTLDTTVKK
                     MEDGSTYVITGDIPAMWLRDSVAQLRPYLVPAQNDPELADLIAGLIRRQFMCINIDPY
                     ANAFNEGPNGNCWEKDETDMGPWIWERKYEIDSLCYPLQFSYLFWKNTGRTDQFDEVF
                     WEGVDKILTVFETEMNHEEKSPYSFIRKNCSYTDTLSRDGKGAQVKSGIGLIWSGFRP
                     SDDSCRYGYLIPSNMFAVVVLNYLKEIADFVGGKEEIAKKAEEMAKTVKQAIETYGTT
                     HIWGLGDVYAYEVDGFGQYNLMDDANVPSLLAMSYLGYEPESQEVADNTRKLILSEAN
                     PFYYSGTKLSGIGSPHTPVRYVWHISKAIEGLTAPTKEEKHQMIHELMATDGGTGLMH
                     EGVFVDDPTVYTREWFSWANAMFCELVMQYCGYEIKK"
     gene            complement(1629..3080)
                     /locus_tag="PLN77_08745"
     CDS             complement(1629..3080)
                     /locus_tag="PLN77_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM44391.1"
                     /translation="MKKKQFLALALSAVMAGGMLAGCGSSNGSSSSSKSDAKDEVVTL
                     KWIQVGNGMPKDYDEWLAQINPYLEEKIGVNVDMEIVPWGDWDNRRSVITNSGEYFDI
                     LFTDQNRYSSEVNTGALMDITDLLKDNASELYDMIPEDYWKAVEVNGKIYGVPTYKDS
                     SLSEYFVWDQDIADKYNIDVNSVTDFNTLYDALKTVKEGEGGSPYFMSKNGANFLLNL
                     NYDDLSSGLPAIGVKYGDDSKTVVNPLDDEEILSNLDIVRKMYQEGIINGDAPTADDS
                     SKYAMFFAAQGWSGAAKTTWGPNNGIANCSAVQYGNTVVSNTTVRGSINGIYSGCKHP
                     DKALQLLNLVNTDSKVRDWFYYGAEGKDFEYTDDNKVHRLTTDWGMAGYTQGTFFNVT
                     QTDDVDFNQWDEVEELNEKATPSEMLGFNLDTSNIETELANCRAVYEKYYSELFTGAQ
                     DPRELVKTIDAELETAGWETIREEAQKQIDAQK"
     gene            complement(3176..4108)
                     /locus_tag="PLN77_08750"
     CDS             complement(3176..4108)
                     /locus_tag="PLN77_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRM44392.1"
                     /translation="MAKKKKDLDLASSSHLNQIQGSTNAIFNVIFVILALACIIPVVF
                     IFMISISSEHSIKMNGYQFIPSAYSLDSYKFLLREGQMILTALGVSVAVTVIGTVIGV
                     ALTMLMGYVLSRSTYKLNGFFTMVVFIPMIFNGGMISSYVVNTQFLNLKNSIWALILP
                     LCVSSFNVVICKTFFKTNIPESIIESAQIDGATQFQIFGKIALPLSKPLMATIALFLT
                     FGYWNDWFQSSLYISDTKLYSLQALLDHVQRNIEMMANNPSLGVTTAQYMNSMPKEGA
                     RMAMAIIIIIPIACCYPFFQKYFISGLTVGAVKG"
     gene            complement(4121..>4459)
                     /locus_tag="PLN77_08755"
     CDS             complement(4121..>4459)
                     /locus_tag="PLN77_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM44393.1"
                     /translation="EAAVMDGANKRQQVWHITLPCLKSVIIMMFILNVGKVFYSDFGL
                     FFQLSQGASGSIFKTTATIDTYVYNALQSSTPIGMTSAATFFQSVACCITILLANAIV
                     KKIDEDSAII"
BASE COUNT         1074 a         1075 c         1036 g         1274 t
ORIGIN      
        1 tatatcttgt acatatcgca aaaaactgtg tataataccc cgtgggcaga gaaaagaagc
       61 gggatgaaaa tcccatattc ccctgggaca gcagtattct ccccactgct gtctctcctt
      121 tcagtgaagg ctgtcgcaga cagttttctg tggtgtaggt aaaagaggag gccgtgcact
      181 gagcacagcc tcttcttttc gtgtttttca tctttatttt ttcatcttta tttcttaatc
      241 tcatatccgc aatactgcat caccagctca cagaacattg cgtttgccca ggagaaccac
      301 tctctggtgt aaacggtcgg atcatctacg aaaacaccct cgtgcatcag tcctgtaccg
      361 ccgtcggttg ccatcagttc gtggatcatc tgatgtttct cctcttttgt cggtgcggtc
      421 agtccctcga tggctttgga gatgtgccat acgtagcgga ccggtgtgtg cggacttccg
      481 attccggaaa gctttgttcc ggaatagtag aacgggtttg cctcgcttaa gatcagcttt
      541 cttgtgttgt cagccacttc ctggctttcc ggctcgtagc cgagatagct catagccaga
      601 agactcggta cattggcatc gtccatcaga ttatactggc caaagccgtc tacctcgtaa
      661 gcataaacat cgccaagtcc ccagatatgg gtggtaccgt aagtctcaat ggcctgtttt
      721 acggtctttg ccatctcttc tgctttcttc gcaatctcct cttttccgcc gacgaaatct
      781 gcgatctcct tcagataatt cagaacgaca accgcaaaca tgttggacgg gatcaggtag
      841 ccgtaacggc aggaatcatc gcttggacgg aagccggacc agatcaggcc gatgccggat
      901 tttacctgcg caccttttcc atcgcgggaa agcgtatcgg tgtaggagca gtttttacgg
      961 atgaagctgt acggggattt ctcctcgtgg ttcatttcag tctcaaatac agtcaggatt
     1021 ttgtcaacac cttcccagaa aacctcgtca aactgatccg ttcttccggt gttcttccag
     1081 aacagatagg agaactgcag cggatagcag agagaatcga tctcatactt tctctcccag
     1141 atccacggtc ccatatcggt ctcgtccttc tcccagcagt tgccgttcgg tccttcgttg
     1201 aatgcgtttg cgtatggatc gatgttgatg cacataaact ggcggcggat caggcctgcg
     1261 atcaggtcag ccagctccgg gtcgttctgc gccggaacaa ggtacgggcg cagctgtgct
     1321 accgagtcac gcagccacat ggccgggata tctccggtga tgacgtaggt gctgccgtcc
     1381 tccattttct ttacggttgt gtcgagggta ttggagtagc atcgttcaaa catatcaacg
     1441 atttccggct cgtctttcag tttttctctc aggcttaaaa tcagctcctg aatggacttt
     1501 ggtaaggtct ctttcattct tttttcatcc tttcgctcaa tttttctcat ttcccgttat
     1561 tctacaacag ggcatctccg caatcgaaaa tgccctgttg tagttcttat aaaattaatg
     1621 tatcagactt atttctgtgc gtcaatctgt ttctgagctt cttcgcggat ggtctcccag
     1681 cctgctgtct caagctcagc atcgatggtt tttacaagct ctcttggatc ctgtgcgccg
     1741 gtgaataact cagaatagta tttctcatat acggcacggc agtttgcaag ctctgtttcg
     1801 atgttggagg tatccaggtt gaatccaagc atctcagacg gagttgcttt ctcgttcagc
     1861 tcttctactt catcccactg gttgaagtca acatcgtctg tctgtgttac attgaagaaa
     1921 gtaccctggg tatatccggc cataccccag tcagtggtca ggcggtgtac cttattgtca
     1981 tcggtgtact cgaagtcttt tccttctgct ccatagtaga accagtcacg tactttggaa
     2041 tccgtattta ccaggttcag aagctgaagt gctttgtccg ggtgcttgca tccggagtaa
     2101 ataccattga tggaaccacg tacagttgtg ttggaaacga ctgtgttgcc atactgtact
     2161 gctgaacaat tggcgatacc attgttcgga ccccatgttg tttttgcagc gccgctccat
     2221 ccctgtgcag caaagaacat tgcgtatttg ctggaatcat ctgctgtcgg agcgtctccg
     2281 ttgatgatgc cctcctgata cattttacga acgatatcca ggttggaaag gatttcttcg
     2341 tcatccagcg gattgacaac ggtcttgctg tcatcgccgt atttaacgcc gattgccgga
     2401 agtccggagc ttaaatcatc gtagttcagg ttcagcagga agtttgcacc atttttggac
     2461 atgaagtacg gagatccgcc ttcgccctct tttactgtct tcagagcatc gtacagagta
     2521 ttgaaatctg ttacagaatt tacatcaatg ttatatttgt ctgcaatatc ctgatcccat
     2581 acaaagtact cggaaaggga gctgtctttg taggtcggta cgccgtagat ttttccattt
     2641 acttctactg ctttccagta atcttccgga atcatgtcat acagctcgga agcgttgtct
     2701 ttgagcagat ctgtaatgtc catcagtgcg ccggtgttaa cctcggaaga gtaacggttc
     2761 tggtctgtga acaggatatc gaaatactcg ccggagtttg tgataacgct tcttctgtta
     2821 tcccagtcac cccacggaac gatttccatg tctacattaa cgccgatttt ctcctccaga
     2881 tatgggttga tctgtgcaag ccactcatca taatccttcg gcattccgtt accaacctga
     2941 atccatttca gtgtaacaac ttcatctttt gcatcactct ttgaagagct gctgcttccg
     3001 ttgctgcttc cgcagcctgc cagcatgcca ccggccataa cagcagaaag tgcaagagct
     3061 aaaaactgtt tctttttcat tgtagcatcc tcctgatata aaatatagaa tgttgaaatt
     3121 atccttaacc gaagcctttt gcttcagtat gagatccata aaggtaaatg gtcgatcagc
     3181 cttttaccgc accgaccgtc agaccggaaa tgaagtattt ctggaagaac ggatagcagc
     3241 atgcgatcgg gatgatgatg atgattgcca tcgccatacg ggcaccctct tttggcatcg
     3301 agttcatgta ctgtgccgtg gtaacgccaa gagacgggtt gtttgccatc atctcaatat
     3361 ttctctgtac gtgatccagc agagcctgca gggaatacag cttggtatcg ctgatgtaca
     3421 gggaagactg gaaccagtcg ttccagtaac cgaaggtgag gaacagggcg atcgttgcca
     3481 tcagcggttt cgacaacggc agggcgatct ttccgaaaat ctggaactgg gttgctccgt
     3541 cgatctgtgc cgactcaatg atagattccg gaatgttggt cttgaagaac gttttacaga
     3601 taacaacgtt gaacgaagaa acacaaagcg gaaggatcag cgcccagata ctgtttttca
     3661 ggttgaggaa ctgtgtgtta actacataag aagaaatcat accaccattg aagatcattg
     3721 gaataaaaac gaccatggtg aagaatccgt tcagtttgta ggtgcttctg gaaagaacgt
     3781 aacccatcag catcgtaagg gcaactccga ttaccgtacc gataaccgtt accgcaacgg
     3841 aaacgccaag tgctgtcagg atcatctgtc cttcccgaag aaggaatttg taggaatcca
     3901 gcgagtatgc actcgggata aactggtagc cattcatctt gatcgaatgc tcggaagaaa
     3961 tcgagatcat gaagatgaat accaccggga taatgcaggc cagggcaagg atcacaaaaa
     4021 taacattgaa aattgcattt gtagatccct ggatctgatt taaatgtgaa gaggaagcca
     4081 gatcaagatc ttttttcttt tttgccattt ttgtacctcc ttaaataatc gcgctgtctt
     4141 catcaatctt cttcacaatg gcattggcaa gaaggatggt gatgcagcat gcaacagact
     4201 ggaagaatgt tgccgcagaa gtcattccga tcggagtgga agactgcagt gcgttgtata
     4261 catatgtgtc gatagttgct gtcgttttga agatcgaacc gcttgctccc tgagaaagct
     4321 ggaagaacag tccgaagtcg gagtagaaga cctttccgac attcaggatg aacatcatga
     4381 tgatgacact ctttaagcaa ggcagggtaa tgtgccatac ctgctgtctc ttgttggcgc
     4441 cgtccataac ggctgcctc
//
LOCUS       DAOMUF010000167         4424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5551_length_4424_cov_4.692149, whole genome shotgun sequence.
ACCESSION   DAOMUF010000167 DAOMUF010000000
VERSION     DAOMUF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5551_length_4424_cov_4.692149"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1004
                     /gene="holB"
                     /locus_tag="PLN77_08760"
     CDS             15..1004
                     /gene="holB"
                     /locus_tag="PLN77_08760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRM44394.1"
                     /translation="MQGFQKVVGHQEIITHLQNAISMNKVSHAYLFGGESGSGKKMMA
                     SLFAMTLQCEKHGVEPCMECPSCKKAQSQNHPDIIYVKHEKPNTISIDEIREQLINDV
                     MIKPYSSPYKIYIIDEAQKLTLQAQNALLKTIEEPPAYAVVMLLADNPDALLPTISSR
                     CVQLNLKPVGDQLVKDYLMNEMHVPDYQAEVDASLAQGNIGKAERIARSPEYEETLEN
                     ALRMAKYADSMPLYEIVETIKKLTAEKDNIDDYFDIFSLWFRDVLLFKATKEVDSLVF
                     KQELNGIRERANKSSYEGLEKIIEAIQKARARLNANVNFDLTMELMFLTMKEN"
     gene            1009..1911
                     /locus_tag="PLN77_08765"
     CDS             1009..1911
                     /locus_tag="PLN77_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HRM44395.1"
                     /translation="MITIIGVRFRNVGKVYYFSPRELDICVGDHVIVETARGVEYGFV
                     VLGPKEVDDSKVIQPLKEVIRIATPKDDAREESNRKKEKEAFEICQKKIRAHNLEMKL
                     IDAEYTFDNNKMLFYFTADGRIDFRELVKDLAAVFKTRIELRQIGVRDETKILGGIGI
                     CGRPLCCHTYLSEFAPVSIKMAKEQNLSLNPTKISGVCGRLMCCLKNEQETYEYLNRK
                     LPGVGDIVTLPDGMKGEVSGVNVLRQLVKVLVDVNDEKEMRECPVEELKFKPKHKKEK
                     GKEKKKDRNSSGEQASSEEAKQPE"
     gene            2081..2764
                     /locus_tag="PLN77_08770"
     CDS             2081..2764
                     /locus_tag="PLN77_08770"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HRM44396.1"
                     /translation="MIQNPSLFCYGIDAVLLASYAKVKRGERAMDFCTGSGIVPILLK
                     AKTNGEHFSGLEIQEYSAEMAQRSVRYNHLENDVEIFQGDVKTASQQFGKEVFDVVTC
                     NPPYMTGQHGLTNALEAKTIARHEVLCSFDDIAKEASAVLKTKGRFYLVHRPFRLAEI
                     FSTLKKYHLEPKRMQLVYPFVDKEPNMVLIEAMKGGNPRITVEKPLIVYETPGVYTKD
                     IRELYGDGR"
     gene            2769..3617
                     /gene="rsmI"
                     /locus_tag="PLN77_08775"
     CDS             2769..3617
                     /gene="rsmI"
                     /locus_tag="PLN77_08775"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRM44397.1"
                     /translation="MAGKLYLCATPIGNLEDMTFRAIRILQEVDLIAAEDTRNSIKLL
                     NHFEIHTPMTSYHEYNKYDKGRELVQKLLAGTNIALITDAGTPGISDPGEELVKMAYE
                     AGIEVTSLPGACACVTALTLSGLSTRRFVFEAFLPPDKKEHKLALERLKNETRTMIVY
                     EAPHHLLKTLRELLETLGNRRLTLCRELTKKHETAWQTTIEDAITRYEQEDPKGECVL
                     VIEGRSQKEIEDEAKAAFEEISIEEHMKRYEDQGIARKEAMKLVAKDRGVTKRDIYQY
                     LLGKES"
     gene            complement(3590..3928)
                     /locus_tag="PLN77_08780"
     CDS             complement(3590..3928)
                     /locus_tag="PLN77_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HRM44398.1"
                     /translation="MKRLSKKQILMLHTQLIQQTGGSDGVRDYNLLDSALETPFQSFG
                     GEDLYPTLQAKAARLGYGLIKNHCMIDGNKRIGTHAMLVFLALNDRTWIFCARSFFSN
                     SIYDSFPSKY"
     gene            complement(3925..4167)
                     /locus_tag="PLN77_08785"
     CDS             complement(3925..4167)
                     /locus_tag="PLN77_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRM44399.1"
                     /translation="MTIDTKTLISISEANQNFSKVARLVDECGSAVILKNNVPRYLVI
                     DFSKAEQETTASDEDVLSISKRLMEQNKEAYEVLAK"
     gene            4315..>4424
                     /locus_tag="PLN77_08790"
     CDS             4315..>4424
                     /locus_tag="PLN77_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM44400.1"
                     /translation="MLSDNIKILRKKKGYSQETLAEQLHVVRQTISKWEKG"
BASE COUNT         1242 a          892 c         1284 g         1006 t
ORIGIN      
        1 cagggggtga ttttatgcag ggttttcaga aggttgtagg acatcaggaa atcataacgc
       61 atttacagaa tgccatttcg atgaataaag tatctcatgc ttatcttttt ggcggagaga
      121 gtggatcggg aaagaaaatg atggcttcac tgtttgcgat gacgcttcag tgcgagaagc
      181 acggggtcga gccctgtatg gaatgcccgt cctgtaaaaa ggcacagagc cagaatcatc
      241 cggatatcat ttatgtgaag catgaaaagc cgaacaccat cagcatcgat gaaatccgcg
      301 agcagctcat taacgatgtg atgatcaagc cgtacagcag tccgtacaag atttatatca
      361 tcgacgaggc gcagaagctg acacttcagg cgcagaacgc gctgctgaag acgatcgagg
      421 agccgccggc ctacgcagtt gtcatgcttc tggcagacaa cccggatgcg ctgcttccga
      481 cgatttcgtc acgctgcgtg cagttaaatt taaaaccagt gggagaccag cttgtgaaag
      541 attatctgat gaacgaaatg catgtgccgg attaccaggc agaggttgat gcgtccctgg
      601 cgcagggaaa tatcggaaaa gcagagcgga ttgcccgttc tccggaatac gaggagacgc
      661 tggaaaatgc actccgcatg gcaaagtacg ccgacagtat gccgctgtat gaaatcgtgg
      721 agacgatcaa aaaactgacg gccgaaaaag acaatattga tgattatttc gatatctttt
      781 ctttatggtt ccgcgatgtt ctgctcttta aggcgacaaa agaggtcgat tcccttgtgt
      841 tcaagcagga gctgaatgga atccgtgaga gagcaaataa gagctcatac gaaggcctgg
      901 agaagattat agaggcgatt cagaaggcga gagcccgcct gaacgcgaat gttaattttg
      961 atttaaccat ggagcttatg ttcctgacaa tgaaggaaaa ctgatagcat gataacaatt
     1021 ataggagtac gttttcgaaa cgtaggaaaa gtatattatt tctcgccgag agagctggac
     1081 atctgcgtgg gcgaccacgt tatcgttgag acagcgcgcg gcgtagagta tggtttcgtt
     1141 gtgctggggc caaaagaagt tgacgattcc aaggtgatcc agccgttgaa agaagtcatc
     1201 cgcattgcaa cgccgaagga tgatgcgcgg gaggagagca accgcaagaa agaaaaagaa
     1261 gcgtttgaaa tctgccagaa gaagatccgg gcgcacaacc tggagatgaa gctgatcgat
     1321 gcagagtata cgtttgacaa taacaagatg ctgttttact ttaccgccga cggaagaatt
     1381 gatttccgtg agctggtaaa ggatctggcg gctgtcttta agacgagaat cgagcttcgc
     1441 cagatcggtg tccgcgatga gacaaagatc ctcggcggaa tcggcatctg cggcagaccg
     1501 ctttgctgcc atacgtatct gtccgagttt gcaccggttt cgatcaagat ggcaaaagag
     1561 cagaacctgt ccctgaaccc gacgaagatt tcgggtgtct gcggacgact gatgtgttgc
     1621 ctgaaaaacg agcaggaaac ctatgaatac ctgaaccgca agctgccggg tgtcggcgat
     1681 atcgtaacgc tgccggacgg catgaaaggt gaggtttccg gtgtgaatgt gcttcgtcag
     1741 ctcgtaaaag tgctggtgga tgtgaacgat gagaaggaaa tgcgggagtg cccggtggaa
     1801 gagctcaagt tcaagccgaa gcataagaag gaaaaaggca aagagaagaa aaaagacaga
     1861 aacagctccg gggagcaggc ttcttcggaa gaggcaaaac agccggaata agattgattt
     1921 ggaatgcatt ttgaaaaaag taagcgccgg gtaaaactga cgcttctttt tgtgtgatgc
     1981 ggcgctgggc ggtcagcagg aggagggatt ttcattggat agaaaagaag aatttttgaa
     2041 gccgggtgag cggctcgatg atctgcagaa cggttactgg atgatacaga atccatccct
     2101 tttctgctat ggcattgacg cggtgctgct ggcatcctac gcgaaggtga agcgcgggga
     2161 gcgggcgatg gatttctgca cgggatcggg aattgtgccg attctgctga aggcgaagac
     2221 aaacggagag catttttccg ggctggaaat tcaggagtac agtgccgaga tggcacagcg
     2281 gagtgtgcgg tacaatcatc tggaaaatga tgttgagatt ttccagggag atgtgaagac
     2341 ggcatcgcag cagtttggaa aagaagtgtt tgatgtggta acgtgcaatc cgccgtacat
     2401 gacggggcag cacgggctga cgaatgcgct tgaggcgaag acgattgcgc ggcatgaggt
     2461 gctgtgctcg tttgatgata ttgcgaaaga ggcatcggcg gttttaaaga cgaaaggacg
     2521 gttctatctg gtgcaccggc cgttccggct ggcggagatt ttttcgacgc tgaaaaaata
     2581 tcatctggaa ccaaaaagga tgcagctcgt ttatccgttt gtggacaagg agccgaacat
     2641 ggtgctgatc gaggcgatga aaggcgggaa tccgagaatt accgtggaga aaccgctgat
     2701 tgtgtatgag acgccgggcg tgtatacaaa ggacatccgg gaattatatg gagacgggag
     2761 ataagaacat ggcaggaaaa ttatatttgt gtgcaacacc aattggaaat ctggaggaca
     2821 tgaccttccg tgcaatccgc attctgcagg aggtcgatct gatcgcggcg gaggatacga
     2881 gaaacagcat taagctgctg aaccattttg agattcatac gccgatgacg agttatcacg
     2941 aatacaacaa atacgacaag ggccgcgagc tggtgcagaa gctgctggcg ggaacaaata
     3001 tcgcgctgat cacggatgcc ggaacaccgg gaatttctga tccgggtgag gagctggtga
     3061 agatggcgta cgaggcaggc atcgaagtga caagcctgcc gggcgcatgc gcgtgtgtga
     3121 cggcactgac actgtcggga ctttcaacga gaagatttgt cttcgaagca tttcttccgc
     3181 cggataaaaa agaacataaa ctggcgctgg aacggctgaa aaacgagacg cggacgatga
     3241 tcgtatacga ggcgccgcat catctgctga aaacgctgcg ggagctgctg gagacgcttg
     3301 gcaaccggcg gctgacactc tgccgggaac tgaccaagaa acacgagacg gcatggcaga
     3361 ccacgattga agatgccatc actcgctacg aacaggaaga tccgaaaggc gaatgcgttc
     3421 ttgtcatcga gggcagaagc cagaaagaaa tcgaggacga ggcgaaggca gcgtttgagg
     3481 agatttcgat cgaagaacat atgaaacggt atgaagatca ggggattgca agaaaagaag
     3541 cgatgaaact cgtggcgaag gatcgcgggg tgacgaagag ggatatttat cagtatttgc
     3601 tggggaaaga atcataaata ctatttgaga aaaatgaccg ggcacagaat atccatgtcc
     3661 ggtcatttaa ggcaagaaaa acaagcatgg cgtgtgtgcc gattcgtttg ttgccgtcaa
     3721 tcatacaatg atttttgatc aggccatagc ccagtcttgc ggcttttgcc tgcagggtgg
     3781 ggtacaagtc ttctccgccg aaggactgga aaggcgtttc gagagcggag tccagcagat
     3841 tgtaatctct gactccgtcg cttccgccag tttgctgaat caactgagta tgcagcatga
     3901 gaatttgctt tttactcagt cttttcattt agcaagtacc tcgtatgctt ctttgttttg
     3961 ttccatcagg cgttttgaga tgcttaacac atcctcgtcg gatgcagtgg tttcctgctc
     4021 ggctttgctg aagtcgatga caagatatct tggaacgtta tttttcagaa tcacagcgga
     4081 accacattcg tctaccaggc gggcgacttt ggaaaaattt tggtttgctt cggaaatgga
     4141 aattaaagtt tttgtgtcga tagtcatttt gggtacctcc ttaagacttt tttctatagt
     4201 atatataaat cttaggataa atacaaccta atttgccgtg ttattttttg ttgcttgccg
     4261 caacgccagt ttttatcgta tgataggccc atcaaatacg cggaggacac agttatgctg
     4321 agtgataaca tcaaaatact gcgaaagaaa aaaggatatt cccaggaaac gctggcggag
     4381 cagctgcatg tggtgcggca gacgatttcg aaatgggaga aagg
//
LOCUS       DAOMUF010000168         4407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5584_length_4407_cov_3.465074, whole genome shotgun sequence.
ACCESSION   DAOMUF010000168 DAOMUF010000000
VERSION     DAOMUF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5584_length_4407_cov_3.465074"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2935)
                     /gene="nifJ"
                     /locus_tag="PLN77_08795"
                     /pseudo
     CDS             complement(1..2935)
                     /gene="nifJ"
                     /locus_tag="PLN77_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587737.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            complement(3241..4191)
                     /locus_tag="PLN77_08800"
                     /pseudo
     CDS             complement(3241..4191)
                     /locus_tag="PLN77_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950232.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(4245..4361)
                     /locus_tag="PLN77_08805"
     CDS             complement(4245..4361)
                     /locus_tag="PLN77_08805"
                     /inference="COORDINATES: protein motif:HMM:NF028446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44401.1"
                     /translation="MLYDEKAKVASRITTKVEVVDGKNVKTRVFKKSGNEVK"
BASE COUNT          912 a         1083 c         1092 g         1320 t
ORIGIN      
        1 atccgatatc gtaagcccat ccgtcaccac cgaagatcca ctgggatttc ttagcgagga
       61 agtctttgtt cttaacgatg tctctgcaaa cgtcgcagtc agcgtctttg attgcttcta
      121 caagtttctc ggttgcagtt ccgttggtag cgccgcagtc aaaggtatcc agatactctt
      181 tgcatgctgc ttttacttct tccggagctt tctcggagtt cataacatcc tcaactttgg
      241 ctttcaggcc gccgcggatt gctttctgag cgagaagcat accataaccg aactctgcgt
      301 tgtcctcgaa cagggagttg gaccatgccg ggccctgtcc tttctcgttt acagtgtaag
      361 gagtggacgg tgaggagtta ccccagatag aggagcatcc tgttgcgttt gcgatgtaca
      421 ttctgtcacc gaacagctgg gtgatcagtt ttgcgtaagg agtctcgccg cagcctgcgc
      481 atgcgccgga gaactcaagc agcggtttct tgaactggct gccctttacg gtattctctt
      541 tgaattttgc tacaacgtct gtcttctctg gaagagctgc tgcataatcg aagtatttct
      601 gctctgcttc gtgtgctgca aggctttcca tagccagtgc tttcacaccc ttcttgcccg
      661 ggcatacgtt tgcacaggaa ccacatccgg tacagtcaag ctgggaaata gccattgtga
      721 aggtgtagtc cggcataccg gtcatcggca tggtctggat gccttccgga gctgctgctg
      781 cttcttctgc ggtcatggca accggacgga taacagcgtg cgggcagacg aaggaacatc
      841 tgttacacta gatacagttt tccggattcc atacaggtac gtttacggcg ataccacgtt
      901 tctcgtatgc ggatgtgccg gacggagttg ttccgtctac gtagtctgca aatgcggata
      961 caggcaggtt gtttccttcc tgggaagtta cttttgcctg gatggtattt acgaatttaa
     1021 cagcgtcagc gcggccgctc tctgcatgag ttgtggtcag accttcgtct gctgcatttt
     1081 tccagctttc cggaactgca acttctttga tctgttttgc accctcatcg attgctgccc
     1141 agttctttgc tacgacatca tcacctttac ggccataggt agcttttgct gctgctttca
     1201 tgagttcgat ggctttttct tccggaatga tcttcgcaag cttgaagaat gcagactgaa
     1261 gaatggtgtt gatacgggtc ggtcccatgc cggtctcgat accgatctta acgccgtcga
     1321 tcacatagaa tttgatgtta tggtttgcga tgaatgcttt tacctgacct ggcagatgct
     1381 cttccagtcc tgcttcatcc catggacagt tcagaaggaa ggttcctccg tctaccagct
     1441 cctctaccat gtgatatttg ttcacatagg aaggattatg acatgcaaca aagtttgctt
     1501 tgtggatcag gtaggtggat ttgatcggtt tctttccgaa acgcaggtga gacatcgtta
     1561 caccgccgga cttcttggag tcataatcga agtaagcctg agcgtacatg tctgtgttgt
     1621 caccgatgat cttgatggag ttcttgtttg cacctacggt accgtctgcg ccaagtcccc
     1681 agaatttgca gttgatggtt ccttccggag ttgttaccag aggagcgcca accggcagag
     1741 acagattggt aacatcgtct acgataccaa tggtaaatct ctgtttctct gtattttcat
     1801 atacagcaac gatctgagcc ggtgtggtgt ccttggaacc aagtccgtaa cggcctgtga
     1861 ataccggtgt gctctcgaat ctgctgcctt ttaatgctgc tactacgtcc aggtacagag
     1921 gctcgccaag agatcccggc tcttttgttc tgtccagaac ggaaatctgt tttacagtct
     1981 ccggaattgc attgatgagt gcttctgcgg agaacggacg gtacagacgt acctttacaa
     2041 cgccgacttt cttgcctgct gctacgaggt aatcgatggt ctcctcgatg gtatcgcaga
     2101 cagaacccat agcgatgatt acgtgctcag cgtctgctgc tccataatag ttgaacagtt
     2161 tgtaatcggt tccgattttc tcgtttactt tgtccatgta ctcctgtacg attgccggaa
     2221 gagcatcgta gtacgggtta catgcttctc ttgcctggaa gaagatatcc gggttctgtg
     2281 cggatcctct ctggcacgga tggttcgggt tcagggcatg tttacggaat gcatctactg
     2341 catccatatc taccatgtct ttcagatctt cgtaatccca tgtctcgatt ttctgaatct
     2401 cgtgggaggt acggaatccg tcgaagaagt tgataaatgg aactttgcct ttcagtgctg
     2461 cacagtgtgc aaccggtgtc aggtccataa cctcctgtac gctggattcg cagagcattg
     2521 cgactccggt ctgacgacag gcgtaaacgt ctgaatgatc tccgaagatg gaaagtgcgt
     2581 ggcttgcaag ggcacgagcg gatacgttga atacgcccgg gagctgctcg ccggcaattt
     2641 tataaaggtt cgggatcata agaagaagtc cctgggatgc ggtgtaggtt gtggtaagag
     2701 ctccggctgc cagtgatccg tgtactgcac ctgctgctcc tgcctcagac tgcatctcag
     2761 ttacctgaac ttcctgtccg aaaatgttct ttcttccctg ggttgcccac tcatcggtat
     2821 gctccgccat aacggatgaa ggggtaattg ggtaaatagc tgctacgtcg gtatacgcat
     2881 aagacgcatg tgctgcagcc tggttaccat ccatggtttt catctttcta gccatttgat
     2941 tttatcctcc ttaaaaaatg atgaccaaaa attactttgg cttttctcag gttggtgttc
     3001 tgccgggttt cggttgcctc cgcgcttcga aaaacccgga taagggcatg gcaatgccac
     3061 tatgtccagt gtttgaatta taacaccgat tgggcattct gtccagattt tctgtgggtg
     3121 ttcagggaaa aatacaagtt ttttacaact tctggtaatt ccgcgtcgaa tttctcgata
     3181 aaaaaagaag gaacggcccg catatttttt tctcttctgc ggacagttcc tttgcttctt
     3241 ttatttctga ttctgcgcct caatctgttc tgcgaggtcg gttagataaa cccatctctc
     3301 ggttttttct tccagagcag cttcggtctt ctctttttcc accatcagtt cggcgagctt
     3361 tgcagagttg gtggcgtttt tgagcatctg atcgtcaagc gatgcaatct tttcctcgag
     3421 tgcggcgata tcgtcatcga tggtctcgta ctcgcgctgt tctttgaacg taaatttcag
     3481 gcgggtcgga cggttctgct tccagtcatt gttctttgac tttgcaggtt cgtccgcgga
     3541 cgttttggac ggcgctgctt ttgccgtttc ttccgcactt ccgccgaaac ggcgttcttt
     3601 tgcttcgagg taatcggtgt agccgccctc gtactgcaga agatttccgt ttccgtcgaa
     3661 ttcgaagatg cggtccacga cgttatccag gaaatagcgg tcgtgggaaa cggtgatgac
     3721 gatgccgacg aacgtgttca ggaaatcctc aagcacggtc agggtcggga tatcaacgtt
     3781 gttggacggc tcgtcgagga tcagaacatt ggcgccgctc actaagacgg ataagagata
     3841 cagtctccgt ttttcgcctc cggagagttt ggaaatcggg gtgtactgca tatccggtgt
     3901 gaacaggaag cgttcgagca tctgcgatgc gctgatgcgg ccgtcacggg tctggacata
     3961 ctcagcgata tctttcacgt agtcgatcac gcgctgattc gtatccatgt ccggctcttc
     4021 ctgtgccaga tagccgatgc ggatcgtgtc gccgatttcg atggttcccg catccggcag
     4081 gacttctccg gtgatcatcc gcagcattgt ggattttccg cagccgttcg ggccaatgat
     4141 gccgacactc tggttccgca ggaagatgta ttcgaaatct ttcatcattt gagggacaac
     4201 tacgttttta tatttttctt gtaaacgatt catcttctac cctcctactt cacttcgtta
     4261 ccgctcttct tgaagacgcg tgtttttaca tttttaccat ctacaacttc aacttttgtt
     4321 gtaatacggc tagcaacttt tgctttttca tcataaagca ttacatttga aacgtgaatt
     4381 ttagcttctt tttcaacgat tccacct
//
LOCUS       DAOMUF010000169         4391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5612_length_4391_cov_4.764991, whole genome shotgun sequence.
ACCESSION   DAOMUF010000169 DAOMUF010000000
VERSION     DAOMUF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5612_length_4391_cov_4.764991"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            653..2416
                     /locus_tag="PLN77_08810"
     CDS             653..2416
                     /locus_tag="PLN77_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44402.1"
                     /translation="MKKNPIGKYLLQNWYYYVFAFGSMALSVFLDMLAPLVTLHIVDD
                     VLVGHKMELLWKYLAAFLLIGAAKALFQYIKEFLFDVSSVRVASQMRGNLFRHVQRLS
                     VEYFDKNNTGELLARVRDDVDRIWDILGFAGMLILEGLICIMMAMVSMVKLDLPLTLV
                     SVAILPFVAGIAIRLEKELDRVYDAISEENAVMNTVAQENLAGVRTVKSFAREPYEIK
                     KFRKHNQKYCDLNMDQARVLMKYDPAISFLTKLMRVLVLLAGGYGVIHGRITLGVLTA
                     FMEYTSKITWPIENVGWLGNCFASAIASNKKLNKILAEEPQIAEPENPVELSKLPGDL
                     EFHHVDFSLHDTPILQDIDFTLKQGHTLGIMGMTGSGKTTIVNLLERFYDVTDGYICL
                     DGHDIRTLPLRTVRDTSSVVMQDVFLFSDTISENVRLGSRSTMKSEEVHNAVSAACAS
                     EFVDHLSDQYETVIGERGMGLSGGQKQRLSIARALAKQAPILVLDDSTSALDMETEYE
                     IQSHLAEKKDVSKIIIAHRISSVQSADEILYLDHGRIAERGTHESLMAQKGLYYSTWE
                     AQYGDYHKAKAALEQALPANA"
     gene            2484..4268
                     /locus_tag="PLN77_08815"
     CDS             2484..4268
                     /locus_tag="PLN77_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44403.1"
                     /translation="MAINSSKVDEEQKEVLKSATIRRLFSYLKNYKRQVAVVLVVLAV
                     TIAISTVNPLLLEYAIDVNIAQKDWRGLVALCVFMVVINLVYAAGVRLRMLLMARITN
                     NILLEIRDELYTHIQTLSFSFFDTRPAGKILARIIGDVNSLKDVLNDGVTKLIPNILT
                     IIAILVIMMIKNIWLSLSAILVLPLIAVGMYFIQIVAHKRWQTFRKKSSNVTAYIHED
                     FSGIRIIKSFTAEQESQGEFDRLLLEHQNSFIHAVRLADGFSAVIEVTWGIGTFLLYF
                     IGISVLGVDAVPIGTFTAFAMYLTMFWDPIQNLAGFYNKLITNLSAAERIFEILDTPA
                     EVADKPGVTELPPIKGEVTFDHVSFAYSDDPDTLVLKDVSFTAAPGETIALVGPTGAG
                     KTTIVNLISRFYNITSGTVRVDGHSLTDVSINSLRAQMGVMTQDNFLFSGTIRDNIAY
                     GKLDATEEEIIAAAKAVHAHDFIIELPDGYDTEISERGARLSNGQRQLLAFARTMVSD
                     PRILILDEATSSIDTQTEIQVQQGIESLLKGRTSFIIAHRLSTIKNADRIFVIDHGKI
                     FEQGTHDELMAKQGAYYQLYQAQFRRVS"
BASE COUNT         1052 a         1210 c         1026 g         1103 t
ORIGIN      
        1 gctcacacct gaatcacttt cttacgcagc gaaacagcaa gtgtttcact gctgattgaa
       61 cagtaatatt atatctcata ttccccaaaa cagctaccca aattcgcaat tttcatacct
      121 tttgcagctt ttatttttta atttttgaaa ataacaattg acaaatccgg aaaacaggag
      181 tagcttagaa ccataagctg tcgtgttcga tcagatgcgg cagaaaaaat catgaaagga
      241 gaacaccact tatgatcaat cttatttatg catccagcgt aaaacagaat aaacttcgta
      301 tggcgatcaa accaggtggc tgcccttctt tcgaaattct ttgtaagcat taactctgca
      361 taacaaatct ctatgcattc tttatgcctg ctatttcgca cagaaggcgc ggtacgttga
      421 tactgcggct ttttttattg ctataaacag tacgaaaccg ttctttccgg cttcgctccg
      481 tttccggcag gcagattccg cggtcttctt accgcatcga ttcccttctt ttatatacaa
      541 acgcagcctt cgctttcgtt tcccgttgtt ccgttcccac acttacggag cttttgaaag
      601 cctatgttct gccagacacc caaacgacat caatgaggag gttttaacat tcatgaagaa
      661 aaatccaatc ggtaagtacc tgctccagaa ctggtactac tacgttttcg cttttggttc
      721 catggcctta agtgtctttc tcgatatgct cgcgccgctt gtcacgctgc acatcgtgga
      781 tgacgtgctg gtcggtcaca aaatggaact gctctggaaa taccttgccg cctttcttct
      841 gatcggtgcc gcaaaagccc tgtttcagta catcaaagaa tttttattcg atgtcagcag
      901 cgtccgcgtc gccagccaga tgaggggcaa cctgttccgc cacgtccagc ggctttcggt
      961 ggaatacttt gacaaaaaca ataccggcga actcctcgcc cgcgtgcgcg atgacgtcga
     1021 ccggatctgg gatatcctcg gctttgccgg catgctgatc ttagaaggcc tgatctgtat
     1081 catgatggca atggtcagta tggtcaaact ggatctgccg ctgacactcg tctccgtggc
     1141 gatccttcct ttcgtcgcag gcatcgcaat ccgcctcgaa aaagaactcg accgtgtcta
     1201 cgatgcgatc agcgaggaaa acgccgtcat gaataccgtt gcacaggaaa atctcgccgg
     1261 tgtccgcacc gtcaaatcct ttgcacggga accgtatgag atcaaaaaat tccggaaaca
     1321 caatcagaaa tactgcgatc tcaacatgga tcaggcgcgc gtcctgatga aatacgatcc
     1381 cgccatctcg tttcttacga agctgatgcg cgtgctcgtc cttctcgcgg gcggctacgg
     1441 tgtcatccac ggacgcatta cgctcggtgt tttaacggcc tttatggagt acacctccaa
     1501 gatcacctgg ccgatcgaaa acgtcggctg gctcggcaac tgcttcgctt ctgcgattgc
     1561 ttccaacaag aagctgaaca aaattctcgc agaggagccg cagatcgcag aaccggaaaa
     1621 tccggtggag ctttcaaaac ttccgggtga cctggaattc catcacgtcg atttctccct
     1681 gcatgacaca ccgattctgc aggatatcga ctttacctta aagcagggcc acacactcgg
     1741 catcatgggt atgacgggtt ccggaaaaac aaccatcgtc aatctgctcg agcgttttta
     1801 cgatgtcacc gacggttaca tctgtctcga cggccacgat atccgcaccc tgccgctgcg
     1861 caccgtgcgc gatacttctt ccgtcgtcat gcaggacgtc ttcctgttct cagataccat
     1921 ttctgagaat gtccgcctcg gcagccgcag cacgatgaaa tccgaggagg tacataacgc
     1981 tgtctccgct gcatgtgctt ccgagttcgt cgaccatctt tcggatcagt acgaaaccgt
     2041 catcggcgaa cgcggcatgg gactttccgg cggtcagaag cagcgtcttt ccatcgcacg
     2101 cgccctggca aaacaggctc cgatcctcgt actggatgat tccacctcgg cgctcgatat
     2161 ggagaccgaa tacgaaatcc agagccatct cgccgagaaa aaagatgttt ccaagatcat
     2221 cattgcccac cgcatttctt cggtgcagag tgccgatgag atcctgtatc tcgatcacgg
     2281 acgcatcgcc gagcgcggca cgcatgagag tctgatggca cagaaaggcc tgtactactc
     2341 cacctgggag gctcagtacg gtgattacca taaagcaaag gcagccctcg agcaggcact
     2401 tcctgccaat gcttaattca atttaatctg ttacccgaca acttctgtat ttacttaccc
     2461 gttaagagag gaataatacg attatggcta tcaattccag taaagtagat gaagagcaga
     2521 aagaggtctt aaaaagcgcg accatccgcc gcctcttctc ctacttaaaa aattataaac
     2581 gccaggttgc cgtcgttctc gtggttctcg ccgttaccat cgcgatttcc actgtgaacc
     2641 cgctgctcct ggagtacgcc atcgacgtca atattgcgca gaaagactgg cgcgggcttg
     2701 ttgccctctg cgtattcatg gttgtgatca acctggttta cgcggccggt gtccgcctgc
     2761 ggatgcttct gatggcgcgc attaccaaca acattcttct ggaaatccgt gacgagctct
     2821 acacgcatat ccagacctta agcttcagct ttttcgatac ccgcccggcc ggaaagattc
     2881 tggcgcgcat catcggcgat gtcaactcgt taaaggatgt gctgaacgac ggtgtcacaa
     2941 agctgattcc gaacattctc acgatcatcg ccattctggt gatcatgatg atcaaaaaca
     3001 tctggctctc cttaagtgcg attctcgtgc tgccgctgat cgctgtcggc atgtatttta
     3061 ttcagatcgt cgcacacaag agatggcaga cgttccggaa aaaatcttcc aatgttacgg
     3121 cgtatatcca cgaggacttc tccggtatcc gcatcatcaa gagctttacc gcagagcagg
     3181 aatcgcaggg cgagtttgac cggcttcttc ttgagcatca gaactccttt atccatgcgg
     3241 tccgcctggc ggatggtttt tccgctgtga tcgaggtgac ctggggcatc ggaacgtttc
     3301 ttctgtattt tatcggtatt tccgtgctcg gcgtggatgc cgttccgatc ggtacgttca
     3361 cggcgtttgc gatgtatctg acgatgttct gggatccgat ccagaacctg gccggttttt
     3421 acaacaagct gatcacgaac ctttccgctg cggagcgaat ctttgagatc ctcgacacac
     3481 cggcggaggt tgccgacaag cctggcgtca cagaactgcc gccgatcaaa ggagaagtaa
     3541 cgttcgacca cgtcagcttt gcctactccg atgatccgga cacgctggtt ttaaaagatg
     3601 tcagctttac agcggctccc ggtgagacga tcgcactcgt cggcccgacc ggcgctggaa
     3661 agacgaccat tgtcaatctg atcagccgtt tctacaatat tacttcggga acggtccgcg
     3721 tcgacggcca cagcctgacg gatgtctcca tcaacagcct gcgtgcccag atgggcgtga
     3781 tgacacagga caactttctc ttctccggca cgatccgcga caacattgcg tatggaaagc
     3841 tcgatgccac ggaggaagaa atcattgccg ccgcaaaggc cgtgcatgcc catgacttta
     3901 tcatagagct gccggacggc tatgacaccg agatcagcga gcgcggtgcc cgtctttcca
     3961 acggccagcg ccagcttctc gcctttgcca gaacgatggt gtctgatccg cggattctga
     4021 ttctcgacga ggcgacgtcc agcatcgaca cacagacaga aattcaggtg cagcagggaa
     4081 tcgaaagcct gttgaagggc cgtacctcgt tcatcattgc gcaccgtctt tctactatta
     4141 aaaatgcgga ccggattttc gtgatcgacc acggaaagat tttcgaacag ggaacccacg
     4201 acgaactgat ggcaaaacag ggcgcgtact accagctcta ccaggcacag ttccgccggg
     4261 tatcctaaaa tcaagaaatt ttctcaaaac tgcctttctt ttttacaggg aaaggcagtt
     4321 tttttccctc ttttctataa ctggttttcc tttccttgac aaatgaatcc tccctgctat
     4381 atgatgataa c
//
LOCUS       DAOMUF010000170         4370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5665_length_4370_cov_2.070915, whole genome shotgun sequence.
ACCESSION   DAOMUF010000170 DAOMUF010000000
VERSION     DAOMUF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5665_length_4370_cov_2.070915"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLN77_08820"
     CDS             complement(<1..365)
                     /locus_tag="PLN77_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HRM44404.1"
                     /translation="MSALKVIQVPAAFNTAGLNIVWILVGLIAIYAGIKNLLDEENPS
                     RIGTAIFWCSFGIVTALGTWLPAKVSGVLVIIMCLPPIFKKVKVGKTNAPEKAHTEAQ
                     FKKIGMKIFVPAFCVAVFSL"
     gene            complement(378..1076)
                     /locus_tag="PLN77_08825"
     CDS             complement(378..1076)
                     /locus_tag="PLN77_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 family protein"
                     /protein_id="HRM44405.1"
                     /translation="MELIKLLGVLIVIVGFALKLDSILIIFLALITTGIVGGLGVGGL
                     LETIGTNFVANRSMAIFMMILLVTGTLERNGLREAAANLIKKVKSATAGKLIAVYGVL
                     NGLFGAFNVGFGGVAGFIRPVLLPMAEGAIENKEGTVNEEHLEEVKGLAAAMQNFTWF
                     FFQVLFVGGAGGILVQTTLAGLGYEVSLIDLAAVEIPVALFSLVVSCVYIIVKDNRIS
                     KKYYEKSAGKKAQK"
     gene            complement(1174..2451)
                     /locus_tag="PLN77_08830"
     CDS             complement(1174..2451)
                     /locus_tag="PLN77_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRM44406.1"
                     /translation="MKGAAEATVKAYAALWSKGVLEKCSCVPAETALVFETESGASYA
                     AQTPEREERPKAKRIENINLHEHELIGYDTYREIIEELKEVPGIEVFRIAVSYTGREL
                     YAVWLKPEYEGYLSLTKRLARVPSEVINARHHANEVASTNASFMLLKKLLTEDVYKEL
                     PDKLNLILIPMENVDGAAIHYELQKEHPTWKFHVARFNSLGKEFYRHYFQQDTIHSEA
                     MGISRIYEKYAPDMMVDNHGVPSHEWEQQFSGYTSPSYKGFWLPRSLLYGYFWYVMNP
                     EYKGNYDVNKVMEDVIADKIAAYPEMKALNQEWSAQFEKYAHAWMPKLFPANYYKEMI
                     NYWIPYESNPAHGYSSIRYPWITTVAYTSEVADETAQGEYLNLCARAHVAHDEVTIQM
                     LMEARNVMDCRFTEQDGTILTSYIRKRPMIVSR"
     gene            complement(2466..>4370)
                     /locus_tag="PLN77_08835"
     CDS             complement(2466..>4370)
                     /locus_tag="PLN77_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44407.1"
                     /translation="VEAALLLGFQAETAVFPVAAADGEIPVPETKEEFRKLWKKTADA
                     APEGYEAASDHIGPVYDLDFREKTGLEKMFQKGPFLKDQNHDFLPDALDVKLVLPEDA
                     SDAMFLAACDLAYRFGMETTGYEGTILAGAGYKGNQIIFEKADQTELVLGETDCDGVK
                     VYLHGDGEALTAFTARLCNTFPKICGERSWKDLLMDMVDDFILRGEDGQLAELKALQE
                     KESGAYAVYGSPRWSEEQKKEAGDAEVYNYKSGQKMYEKVYEFPWEVEVFEELLETEV
                     YPKLKEGSTVKITGAVSETESVRRKMCEAIDAKIREAGAVPEQTTIYCAYKQGYSWLA
                     EEMLPRLKEAGADTVTVYFKPFLPEGQTEWNDENGAIPSYHNLSAETPDKWYDLPIRY
                     LQELYPIEDLLVKELGIERKNVVFKAYEGEDDITYLCQGSKENSVCCEDAYKAAWSER
                     PYMNEYPQMGKVHPSTGYIKAEVNGKTILDKKVRTDLEEIWDVYQSEVLPDCRRYIEE
                     KTGGTVAEEMQPFFHELRMDITVSEPDEPTGSREDLISSLDALHEDMYFVGGDYFKNY
                     GIQKAGVMLDAPGLILPVIHQKEGRPVFRVTLTEPLNASQKTERPLPQRENEVKSKPG
                     FPQFRGKTES"
BASE COUNT          983 a         1210 c          939 g         1238 t
ORIGIN      
        1 agggaaaata cagcaacaca gaatgccgga acgaaaatct tcattccaat cttcttaaac
       61 tgtgcttcgg tgtgtgcttt ttccggtgcg tttgtttttc caacctttac tttcttgaag
      121 atcggcggca gacacataat aataacaagg acaccggaaa ccttggccgg aagccaggtg
      181 ccaagtgctg taacaatacc gaacgaacac cagaaaatgg ctgttccgat tctggacgga
      241 ttttcttcat ccagaaggtt tttaatgccc gcgtagattg caatcaggcc aacgagaatc
      301 catacaatat ttaatccggc cgtattaaat gcggctggaa cctgtataac ttttaatgcg
      361 ctcatctagt gttcctccta tttctgtgcc tttttgccgg ctgatttttc atagtatttt
      421 ttcgaaatcc gattgtcttt tacaatgata tagacacagg aaacaaccag tgagaataat
      481 gcaaccggaa tttcaactgc cgcaaggtca atcaggctta cttcatagcc aagaccggca
      541 agtgtcgtct gtaccagaat tccaccggca ccgccgacaa acagaacctg gaagaagaac
      601 caggtgaagt tctgcattgc tgctgcaagt cctttgactt cttctaagtg ctcttcgttt
      661 acggtacctt ctttgttctc gatcgcacct tctgccatcg gaagaagaac cggacggatg
      721 aatccggcaa caccgccgaa tcctacgttg aatgcaccga acaggccatt taatacgccg
      781 tatacagcga tcagcttgcc ggctgttgca ctcttaactt tcttaatcag gtttgctgct
      841 gcttcacgaa gaccgtttct ttcaagcgta ccagtaacga gcaggatcat catgaagatc
      901 gccatgcttc ggttcgctac gaagttggtt ccgatggtct caagaagacc gccgacgcca
      961 aggccaccaa cgatccctgt cgtaatcagc gccaggaaaa tgatgaggat tgaatccagt
     1021 ttcagcgcga atccgacgat aacgattaac acgccaagta atttgatgag ttccatacct
     1081 aaactcctcc ttaaataaaa tgtagttttt tttggataca gactgctccg cacgctcgtg
     1141 ctttcacaac cctgtatcct gtatttcttc cagttaccta gagacaatca tcggtctctt
     1201 tcgtatgtaa ctggtcaaaa tcgttccatc ctgctcggta aagcggcagt ccataacgtt
     1261 ccgcgcctcc atgagcatct gtattgtcac ttcatcgtgc gccacatgag cccgcgcaca
     1321 caggttcagg tattctccct gtgccgtttc atccgcaacc tcgctcgtat aagcgacggt
     1381 tgtaatccac ggatagcgga tcgacgaata tccatgcgcc ggattcgatt catacggaat
     1441 ccagtaattg atcatttctt tataataatt ggccggaaac agtttcggca tccacgcatg
     1501 ggcatatttt tcaaactgtg cactccactc ctggttcagt gctttcatct ccggatacgc
     1561 tgcaatcttg tctgcgatca catcctccat gaccttattc acatcatagt ttcctttgta
     1621 ttccggattc attacatacc agaaataacc atataagagc gatctcggaa gccagaaacc
     1681 tttgtaggac ggggaggtat aaccagaaaa ctgctgctcc cattcgtggc ttggcacacc
     1741 gtggttatcc accatcatat ccggtgcata tttctcgtaa atccggctga ttcccatcgc
     1801 ttcgctgtga atcgtatcct gctggaaata atgacggtaa aactcttttc caagagaatt
     1861 gaaccgcgcc acatggaatt tccacgtcgg atgctccttc tgcagttcgt agtgaatcgc
     1921 tgcaccgtcc acgttttcca tcgggatcag gatcagattc agcttatccg gcagctcttt
     1981 gtaaacatct tctgttaaaa gtttcttcag cagcataaac gatgcattcg ttgaggcaac
     2041 ctcgtttgca tggtgtcttg cattgatcac ctcgctcggc actctcgcca gacgctttgt
     2101 cagcgacaga tatccctcgt actccggttt cagccagact gcatagagct cgcggcctgt
     2161 ataggaaacg gcaatccgga agacctcgat acccggaact tctttcagct cctcaatgat
     2221 ctcccgatac gtatcgtatc cgatcagttc gtgctcatgc aggttgatgt tctcgatccg
     2281 tttcgccttt ggcctttctt cccgctccgg ggtctgtgcc gcatacgatg cgccgctttc
     2341 tgtttcaaag acaagcgctg tttccgcagg aacacagctg catttttcaa gaacaccttt
     2401 cgaccacagc gctgcgtatg cttttacggt agcctctgct gcgcctttca ctgtgatatg
     2461 gaaattcagc tctccgtttt cccacgaaac tgcggaaatc caggtttcga cttcacttcg
     2521 ttttctctct gcggcagcgg tctctccgtc ttttgtgatg catttaacgg ctctgtcagc
     2581 gtaacgcgaa aaaccggccg gccctctttc tgatggatca ccggcagaat caggcccggt
     2641 gcatccagca tcacgccggc tttctgaatg ccgtaatttt taaaataatc accgcccaca
     2701 aaatacatat cctcatggag tgcatcaagc gatgagatca gatcctcacg ggaaccggtc
     2761 ggctcgtccg gctcgcttac cgtaatgtcc attctcagtt cgtggaagaa cggctgcatc
     2821 tcctccgcaa cggttccgcc cgttttttcc tcgatatagc ggcggcagtc cggaaggact
     2881 tcgctctggt agacatccca gatttcttcc agatcggtgc ggactttttt gtcaagaatg
     2941 gtcttgccat ttacttctgc ttttatataa ccggtcgacg ggtgaacttt tcccatctgc
     3001 gggtattcat tcatatacgg acgctccgac cacgcggcct tgtacgcatc ctcacagcag
     3061 acgctgtttt ctttgcttcc ctggcagaga tatgtgatgt catcttctcc ctcataggct
     3121 ttgaatacga cattttttct ctcgataccg agctctttta ccagaagatc ctcgatcggg
     3181 tacagctcct gcagataacg gatcggtaag tcgtaccatt tgtccggcgt ctcggcactc
     3241 aggttgtggt aagacggaat ggctccgttt tcatcattcc attccgtctg gccttctggg
     3301 agaaacggtt tgaaataaac ggtaaccgta tcggctccgg cctctttcag acgcggaagc
     3361 atctcttctg ccagccagga atagccctgt ttgtaggcac agtaaatcgt tgtctgttcc
     3421 ggcacagcgc ctgcttcgcg gatttttgca tcgatcgctt cgcacatctt ccggcgcacg
     3481 ctctcggtct cgctaactgc tccggtgatt tttacggtgc ttccctcttt gagtttcgga
     3541 tagacttccg tttccagaag ttcttcaaat acttcgacct cccacgggaa ttcatatact
     3601 ttctcgtaca ttttctgacc ggatttgtaa ttgtaaactt ctgcatctcc ggcctctttt
     3661 ttctgctctt cgctccagcg cggcgatccg taaaccgcat aggcgccgct ttctttttcc
     3721 tgtaaagctt tcagttcggc aagctggccg tcctctccgc gtaggataaa atcatccacc
     3781 atgtccatca ggagatcttt ccagcttctc tcaccgcaga tctttggaaa tgtattgcag
     3841 aggcgggcgg tgaatgcggt cagtgcttcg ccgtctccgt gcagatacac ttttacgccg
     3901 tcacagtctg tctctccaag gaccagttct gtctgatctg ctttttcaaa aataatctga
     3961 tttcccttgt aacccgcccc ggcaagaatg gttccttcgt atcctgtcgt ctccatcccg
     4021 aaacggtatg ccagatcaca cgctgcaaga aacatagcat cgcttgcgtc ctctggaagc
     4081 accagtttta catcgagtgc atccgggagg aaatcgtgat tctgatcttt taaaaacgga
     4141 cctttttgga acattttttc caatcctgtt ttttcccgga aatccaggtc gtaaaccggc
     4201 ccgatgtggt ccgaagccgc ctcatacccc tccggggctg catctgctgt tttcttccac
     4261 agcttccgga attcttcttt tgtttccggt accgggattt ctccatccgc ggctgcaaca
     4321 ggaaacactg ccgtctctgc ctggaaacca agaagcagcg ccgcctcaac
//
LOCUS       DAOMUF010000171         4366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5669_length_4366_cov_3.199954, whole genome shotgun sequence.
ACCESSION   DAOMUF010000171 DAOMUF010000000
VERSION     DAOMUF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5669_length_4366_cov_3.199954"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /gene="rlmD"
                     /locus_tag="PLN77_08840"
     CDS             complement(<1..1397)
                     /gene="rlmD"
                     /locus_tag="PLN77_08840"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRM44408.1"
                     /translation="MSYQKNDVLTVKIEDMGHDGEGIGKCEGYTLFVKDTVIGDLAEV
                     KVIKAKKNYGYARLMRLIEPSAHRVEPVCPVARPCGGCQLQMLDYAEQLRFKEKKIAG
                     NLQRIGGMTEIPMEPIVGMENPFRYRNKAQFPIGQDRNGKLITGFYAGRTHQIMENRN
                     CYLGVEQNEEILNRILAWMEENGVPAYNEETGKGLVRHVLIRFGFMTKEIMVCLVVNG
                     KKLPAEEKLIKSLTELEGMTSITLSANRERTNVIMGKEIRCLWGQGYITDYIGTVKYR
                     ISPLSFYQVNPAQTEKLYGLALEYAGLTGNETVWDLYCGIGTISLFLAQKAKKVYGVE
                     IVPEAIADAKNNAKINEIENAEFFVGKSEEILPAYYEQYAKDHPGEHAHADVIVVDPP
                     RKGCEENLLHTMASMQPDRIVYVSCDSATLARDVKVLGELGYEVKKVRGVDQFPMTCH
                     VETVVLLSQQKPDDT"
     gene            complement(1394..2344)
                     /locus_tag="PLN77_08845"
     CDS             complement(1394..2344)
                     /locus_tag="PLN77_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM44409.1"
                     /translation="MKYINELREGNRISGIYLCKHKQSAVTKNGKQYENVILQDKTGT
                     IDAKIWDPNSMGIREFEALDYVDIMGDVSSFNGALQVSIKNARKAEEGEYNPADYLPT
                     SRYDINTMYQELLSWIGTVKNQYLSELLTYYFIQDQETAKRFRMSSAAKSVHHGFVGG
                     LLEHTLSVTRFCDFMVKSYPILNRDLLITAAILHDIGKTKELSLFPQNDYTNDGQLLG
                     HIMIGAEMVHDAAQKIDGFPQELENQLKHCILAHHGELEYGSPKKPAMVEAVALNLAD
                     NADAKMETLTELFDAAPAGNEWLGYNRFFESNIRRTGDFS"
     gene            complement(2582..4036)
                     /locus_tag="PLN77_08850"
     CDS             complement(2582..4036)
                     /locus_tag="PLN77_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRM44410.1"
                     /translation="MENDPKPYKFMKESIKKQPPDWKKIVLLIAGWLALAALGGLVAA
                     AVFAVTEPKIAEAVTREEPPAKVDIPGDEDPNSGQEPDETITASSASASVDSSGSGSE
                     ISSSTVDSSTSESSVSESTVSESTEENGAAGGTESSTSEEPEEGSEVSSVDGETDAEE
                     KDSSLKNYEALYQDMLEVTEKPKRALVTVIGITNQMDYFNQNYENQQQISGLIVADNG
                     QDLFILTEYRIVENVERIQVTFWDETMVDATYQRHDPSTGLTIVKVDESKLDEETRDG
                     LAVAPLGSSYLVSQGDPVVAVGSPVGYSNSIAYGVVTSVTNKISALDNEYNLLTTDIL
                     GSTDGSGILVNLDGEIVGIIAQSYSAKGNNVVTGIAISQIKKLIENLSNNVSRAYIGI
                     RGQDVTEELSDKTGIPKGVLISRVADDSPAMMAGMKEYDVIVKLGEHKVETIKQYHEQ
                     LGKYSTGDVVTVTAMRKGAEGYAEMTFDVTMGEV"
BASE COUNT          894 a         1224 c          989 g         1259 t
ORIGIN      
        1 atcgtgtcat ctggtttttg ttgggacaaa agaacaaccg tctccacatg gcacgtcatc
       61 gggaattggt caacccctcg gactttcttt acttcatacc ccagctcccc aagcaccttc
      121 acatctcgtg ccagcgttgc ggaatcgcag gatacataca cgatgcggtc cggctgcatg
      181 ctggccatgg tgtggaggag gttttcttcg cagccttttc tcggcggatc gacgacgatg
      241 acgtccgcgt gggcgtgttc gcccgggtgg tctttggcgt actgttcgta gtaggccgga
      301 agaatctctt ccgattttcc aacgaaaaat tcagcgtttt cgatctcgtt gattttcgca
      361 ttgttcttcg catccgcgat tgcctccggg acgatttcga cgccgtagac ttttttggct
      421 ttctgcgcga ggaacaggga aatcgttccg atgccgcagt agagatccca gaccgtctca
      481 tttccggtga gacctgcgta ttccagggca aggccgtaca gcttttccgt ctgcgccggg
      541 ttgacctggt agaaggacag cggggagatc cgatatttta cggttccaat atagtcggtg
      601 atatacccct gtccccacag gcagcggatt tcttttccca tgatcacatt ggtccgctca
      661 cggtttgcgc ttagggtaat gcttgtcatg ccttcgagtt ctgtcagcga tttgatgagt
      721 ttctcctcgg ccggcagttt ctttccgttc acaacgagac agaccataat ctctttcgtc
      781 ataaatccaa accggatcag cacatggcgc accaggcctt tgccggtctc ctcgttgtat
      841 gccggaacgc cgttctcctc catccaggca agaatgcggt tcagaatctc ctcgttctgc
      901 tccacgccga gataacagtt ccggttttcc atgatctgat gcgttcttcc cgcataaaat
      961 cccgtaatca gctttccatt tctatcctgc ccgatcggaa actgggcttt gtttcgatag
     1021 cggaacggat tttccatccc cacgatcggc tccatcggga tctccgtcat gccgccgatg
     1081 cgctggagat ttccggcgat tttcttctcc ttaaagcgaa gctgctccgc atagtcgagc
     1141 atctgcaact ggcatccgcc gcacggacgt gcaaccgggc agaccggctc gacgcggtgt
     1201 gcggacggct cgatcaggcg catcaggcgc gcgtagccgt aatttttctt tgctttgatc
     1261 acttttacct cggcaagatc gccgatgacg gtgtccttga caaagagggt atagccctcg
     1321 catttgccta taccctctcc gtcgtgtccc atatcttcaa ttttcaccgt cagtacatca
     1381 tttttctgat agctcatgaa aagtctcctg ttctgcggat gttggactca aagaaccggt
     1441 tgtagccaag ccactcattg ccggccggcg ctgcgtcaaa cagctcggtc agggtttcca
     1501 ttttggcatc ggcattgtcc gccaggttca gcgctaccgc ctctaccatt gccggttttt
     1561 tcggggaacc gtattccagc tcgccgtggt gcgccagaat gcagtgcttt aactgattct
     1621 cgagttcctg tgggaaaccg tcgatcttct gcgccgcatc gtgtaccatc tccgcaccga
     1681 tcatgatgtg gccgagcagc tgtccgtcat tggtgtaatc gttctgcggg aataaggaga
     1741 gttctttcgt ctttccgata tcgtgcagaa tcgctgccgt aatcagcaga tcgcggttca
     1801 gaatcggata cgattttacc ataaaatcgc agaaacgggt gacacttaac gtatgctcta
     1861 aaaggccgcc gacgaatccg tgatgcacgc tttttgctgc agagctcatg cggaaccgtt
     1921 ttgcggtctc ctgatcctgg ataaaataat acgtcagcag ttcggagaga tactgatttt
     1981 tcaccgttcc gatccaggag agcagctcct gatacatcgt attgatgtcg taacggctcg
     2041 ttggcagata gtccgccgga ttatattcgc cctcttccgc ttttcttgca tttttaatgg
     2101 aaacctgaag tgctccatta aaactggaaa catctcccat aatatctaca taatccagtg
     2161 cttcaaattc acggattccc atagaattcg gatcccagat tttcgcatcg atcgtaccgg
     2221 ttttatcctg taagatcacg ttttcgtact gttttccgtt tttggtaaca gccgactgtt
     2281 tatgtttgca gagataaatt ccggaaatgc ggtttccttc ccggagttcg ttgatatact
     2341 tcataaaaat aaatcctctt tttctaaaat aaatgcggac acagtgttcc gtttttgttt
     2401 ctatgatgcc gtttttatga tgcaaagcct gtgctcctgc atgccttacc aattctacca
     2461 gattttcctg cagaatgcac gtattttttc atccgccagt ggaaaaccat tcgaaaagaa
     2521 aagcttccgg aagccccacc ggctaaaacc ggctttttct gctcccgaaa gcctctgttt
     2581 tttatacttc ccccatcgtc acatcaaacg tcatctccgc atacccttcg gcgcctttcc
     2641 gcatcgccgt cacggtaacc acgtctcctg tgctgtattt gccaagctgt tcatggtact
     2701 gtttgatcgt ctcaaccttg tgctccccga gctttacaat cacatcgtac tctttcatgc
     2761 ccgccatcat cgccggagaa tcgtctgcga cgcggctgat gagaacgcct tttggaatgc
     2821 cggttttatc ggaaagttct tctgtgacat cctgtccgcg gatgccgata tacgcgcggc
     2881 tcacgttgtt ggaaagattt tcgatcagct ttttgatctg ggaaatcgca atgcccgtca
     2941 ccacgttgtt tcctttggcg ctgtaactct gcgcaataat gccgacgatt tcgccgtcaa
     3001 gatttaccag aatgccgctg ccgtccgtgc tgccgaggat gtcggtggtc agcagattgt
     3061 actcattgtc cagcgcggaa atcttgttcg taaccgaagt caccacaccg tacgcaatcg
     3121 aattgctgta gccgaccggg cttccgacag ccacgaccgg gtcaccctgg ctgacgagat
     3181 acgaactgcc gagcggcgca accgcaagac cgtcccgtgt ttcctcatcc agcttcgatt
     3241 catccacctt cacaatcgta aggccggtgc tcggatcgtg gcgctggtat gtggcatcca
     3301 ccatcgtctc gtcccagaag gtgacctgaa tccgctccac attttccacg atgcggtatt
     3361 ccgtcagaat aaagagatcc tgcccattgt ccgcaacaat cagaccggaa atctgctgct
     3421 ggttttcata gttctggtta aaatagtcca tctgattggt aatgccgatg acagtaacca
     3481 gcgctcgttt tggtttttcc gttacttcca acatatcctg gtagagcgct tcataatttt
     3541 tcaggctgct gtccttttcc tccgcatccg tttccccatc gacactggaa acctcgctgc
     3601 cctcctccgg ctcttcggaa gtgctgcttt ccgttccccc tgccgctccg ttctcctctg
     3661 tgctttcgga aaccgtactt tcggatacac tgctttctga agtactgcta tccactgtag
     3721 aactgctgat ttccgaaccg gagccgctgc tgtctaccga cgcactcgcc gaagatgcgg
     3781 taattgtctc atccggctcc tgtccgctgt ttggatcctc atctcccggg atatccactt
     3841 tcgccggcgg ctcttccctg gtaactgctt ctgcaatctt tggctctgtc accgcaaata
     3901 ccgcggccgc aaccagaccg ccaagcgccg caagtgccag ccatccggca attagaagaa
     3961 caatcttttt ccagtcgggc ggctgctttt tgatgctctc tttcatgaat ttgtaaggtt
     4021 ttgggtcatt ttccatacat ttctttctcc atacatctgt attccatacc gaactgctct
     4081 gcgcatatgc gcttccgcaa tcttcacata tataaaaaac tttaccagat agatatgaac
     4141 attttatgaa gactgttaca tttttttctc atttgcgcaa catcctgcat tttctcgttc
     4201 ttggggtttc tttcttttct tttttaaggt ataattgggg gagtggaaaa acctgtgacc
     4261 cacattccac ttccggtctg tacttcccgt gcagatatat gatcagatcg ctactgttca
     4321 ctcagcagtg aaacacttgc tgtttcgctg cgtaagaaag tgattc
//
LOCUS       DAOMUF010000172         4342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5710_length_4342_cov_4.032190, whole genome shotgun sequence.
ACCESSION   DAOMUF010000172 DAOMUF010000000
VERSION     DAOMUF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5710_length_4342_cov_4.032190"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLN77_08855"
     CDS             complement(<1..294)
                     /locus_tag="PLN77_08855"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRM44411.1"
                     /translation="MEEERKIEELQRLIDTHENIVFFGGAGVSTESGIPDFRSVDGLY
                     HQKYDVPPETILSHSYFLRYPEKFYAFYRDKMLPVGAKPNAAHKKLAELEAAGK"
     gene            complement(321..1172)
                     /gene="srtB"
                     /locus_tag="PLN77_08860"
     CDS             complement(321..1172)
                     /gene="srtB"
                     /locus_tag="PLN77_08860"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HRM44412.1"
                     /translation="MGKQKKKQKKRGNPLWTLVFLAALAVFCFSGYKLIGIYLDYKTG
                     TDEYRDLQQYTTEITKTPETPATTKTEEPQAESEPAEPSEPEPEPLSYPTEPPLAVDF
                     ESLKAINPDVKGWLYIEALDISYPVVQGPDNDAYLHTTYEGTSNFAGSIFLDYQNQGD
                     FSDGNTIVYGHNMKNLSMFGKLKQMKEQEKYRDSVYFWMLTPESNDVYQIFSAFYTEA
                     DSDVYTLYSGGGEAFVQYLNTMAARSEIPVEQPEMDENSHIIVLSTCAASDTTGRFVV
                     LGVRRNR"
     gene            1380..2444
                     /locus_tag="PLN77_08865"
     CDS             1380..2444
                     /locus_tag="PLN77_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM44413.1"
                     /translation="MRTIKKVASHIFIDGFGGMTQGLFATLIIGTMIQQIGLLIGGST
                     GNVIYLLGKAAAALTSAGIGVGVAVKYGESPLVTVSAAVAAMVGGYAGKITGGTFFVD
                     GNAVLAGPGEPLGAFIAAFAGITCGHLVSGKTKVDIIVTPVITIGAGSVVGLLVGPPI
                     SQMMTGLGSIINWATEQRPFIMGIVVSVVMGMVLTLPISSAALGIILNLSGLAAGAAT
                     IGCCCNMVGFAVASYRENKFGGLVAQGLGTSMLQVPNIMRHPLIWLPVIFSSAILGPV
                     STILANMQNNATGSGMGSAGLVGQITTYQTMIAYDDPKLVIIKIILLHFVLPAVITLF
                     FSEVFRKANLIKSGDMALNL"
     gene            complement(2532..>4342)
                     /locus_tag="PLN77_08870"
     CDS             complement(2532..>4342)
                     /locus_tag="PLN77_08870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRM44414.1"
                     /translation="KTDFAPQKEAFGDFASPETYFPRVVPESEGISSEKLTQMLRELA
                     AARHTDMHHLLVLRNGHVICECNFAPYRSGIWHATYSMCKSITGMAVGFLISEGKLSL
                     DENVYDIFEKRNGLLQKILRPNLTVEHLLTMKSGVQFNEMGVVSGNDWVDSFLNAPVK
                     GTPGEAFEYNSMNTYLLSAIIQERTGMKMVDYLRPRLFEPLGIRKVFWESCPAGITKG
                     GWGLFLCPEDAAKLGVMYVNGGKFEGKQIVPAEWVAASTSVHATPPEKMGKYGYGYQV
                     WMEERPGSFAFNGMLGQNVLGYPDTGVVIVTNAGSNELFQTCEMLDIVRKYFGAEFGA
                     ELKSGEAESPMAYQKLVQTCRDLEGAKETPGRILRGGWKRRTPGPRNSGTPRQIDMAQ
                     LLHGKEYKMEDTHVGMFPLTLQVFHNNFSDGIRRIGFFYEKGRFFVELTEGEKTQRIE
                     IGLTGSKVVEWVENEESYLLGTTGQFAENEDGELVLKLEFAFLEEAARRRVKIIFQRD
                     FERIRLEWNETPGKDLIIEGLESLVTDVAIAPLLPSRFRERSLDMIHLLMAQTIEPMV
                     EAHRVRPGEETETEEAAAEAESAAAVENAENAEETV"
BASE COUNT          894 a         1293 c         1029 g         1126 t
ORIGIN      
        1 ttttcccgcc gcttcgagct ctgcaagttt cttatgcgcc gcgttcggtt tcgcgccgac
       61 cggaagcatt ttgtcgcggt aaaaagcata aaacttttcc gggtaccgca ggaaatagct
      121 gtggcttaaa attgtctccg gcggaacgtc atacttctga tgataaaggc catccacgct
      181 ccggaaatcc ggaatcccgc tctccgtgga cacgcccgcg ccgccgaaga atacgatatt
      241 ttcatgcgta tcaatcaatc tctgcagttc ttcgattttt ctttcctctt ccatctgtca
      301 ttcctccttc tgaccaatca ttaccggttc cgccgcacgc ccagaacgac gaaccgtcca
      361 gtcgtatcgc tcgccgcaca ggtggacagc acaataatat ggctgttttc atccatctcc
      421 ggctgctcca ccgggatctc tgaccgcgcc gccatggtat tcagatactg tacaaacgct
      481 tcacccccgc cggaatacag cgtgtagaca tcactgtctg cctccgtata aaaggcagaa
      541 aaaatctggt acacatcgtt tgattccggc gtcagcatcc agaaatagac gctgtcccta
      601 tatttttcct gctccttcat ctgtttcaat tttccgaaca tggagagatt cttcatatta
      661 tgaccgtaaa cgattgtgtt tccatcactg aaatcaccct gattctggta atcgaggaaa
      721 atggaacccg caaagttgct cgttccctcg tacgtcgtat gaagatacgc atcgttgtcc
      781 ggcccctgta cgaccgggta actgatgtcg agagcttcga tataaagcca tcccttcaca
      841 tccgggttaa tcgcttttaa ggattcaaaa tcgacggcaa gcggcggttc tgtcggatac
      901 gacagtggtt ccggctccgg ttcggaaggc tccgccggtt cggattccgc ctgcggctcc
      961 tctgttttcg tcgttgccgg tgtctccggt gtttttgtaa tttccgtcgt atactgctgc
     1021 agatcccggt attcatccgt gccggtcttg taatccaggt aaatgccgat cagcttgtac
     1081 ccggaaaagc agaaaacagc aagtgccgct aaaaatacca gagtccaaag cggattgccg
     1141 cgttttttct gtttcttttt ctgttttccc atgcgtctcc tttatcactt ttttactgtc
     1201 tgcgctgttt tctggaagcg ctctgaattt gtctcaatta taagcgtgcc cctttctttt
     1261 gtcaacttat gcccccggaa aaggattgca cccgccccca tcttatgata gactaaaagt
     1321 cgactgacaa gtcacttgtg aatacagtaa cacaccacat acatgaggag gaattccata
     1381 tgcgaacaat caaaaaagtc gcctcgcaca tcttcattga tggcttcggc ggcatgacgc
     1441 agggtctttt tgcaaccctg attatcggta cgatgattca gcagatcggt cttctgatcg
     1501 gcggttcgac cggaaatgta atctatctgc ttggaaaagc tgccgcagcc ctgacaagtg
     1561 ccggtatcgg tgtcggcgtg gcagtgaaat atggggaaag cccgcttgtc acggtttctg
     1621 cagccgtcgc cgccatggtc ggcggttatg ccggcaagat caccggcggc acattctttg
     1681 tggacggaaa tgccgttctg gcaggacctg gcgaaccgct cggcgctttc atcgcagctt
     1741 tcgcaggcat cacctgcggc catctggttt ccggcaagac caaggttgat atcatcgtga
     1801 caccggtcat caccatcgga gccggttccg ttgtcggact gctcgtcgga ccgccgatct
     1861 cccagatgat gacaggcctt ggaagcatca tcaactgggc aacggaacag cggccgttca
     1921 tcatgggcat cgttgtttcg gtcgtcatgg gtatggttct gacactgccg atcagctccg
     1981 ctgcactcgg catcattctg aatctttccg gtcttgccgc cggtgccgca acgatcggct
     2041 gctgctgcaa catggtcgga ttcgcggttg caagttatcg ggaaaacaaa ttcggcggcc
     2101 ttgtcgcaca gggtcttgga acctcgatgc ttcaggtgcc gaacatcatg cgtcatccgc
     2161 tgatctggct acccgtcatc ttctcgagtg cgattttagg acccgtctcc acaatcctcg
     2221 ccaatatgca gaataatgca acgggaagcg gcatgggttc cgcaggtctt gtcggacaga
     2281 tcacgacgta tcagacgatg atcgcttacg atgatccgaa gctcgtcatc atcaaaatca
     2341 ttctgctcca cttcgtgctt ccggctgtga tcaccttgtt cttctcagag gtattccgca
     2401 aggcaaacct gatcaaatcc ggcgatatgg ctttgaatct gtaatctgta tttttaaaag
     2461 ggaatggaac tgcgcatccg gcagctccat tccctttttt atccacattt ctcttctgat
     2521 tttttctgtt tttacaccgt ttcttctgcg ttttccgcat tttccactgc cgcggccgat
     2581 tcagcttccg ctgccgcttc ttctgtctcc gtttcttccc ccggccgcac acggtgtgcc
     2641 tctaccattg gctcaatggt ctgtgccatc agcagatgga tcatgtcaag gctccgctcg
     2701 cggaaccgcg acggcagaag cggtgcgatc gccacatccg tcacgaggga ttcaaggccc
     2761 tcgataatca gatccttgcc cggcgtctca ttccactcca gtctgatccg ctcgaaatca
     2821 cgctggaaaa tgattttgac cctccgtctt gcggcctctt ccagaaatgc aaactcaagc
     2881 ttcagcacca gttcgccgtc ttcattctcc gcaaactgtc ccgtcgttcc gagcagatac
     2941 gattcctcgt tttccaccca ttctacaact ttggaccccg taagaccgat ctcaatgcgc
     3001 tgtgtttttt ctccctcggt cagctcgaca aagaaacggc ctttttcata gaagaaaccg
     3061 atgcggcgga tgccgtcgga aaagttgttg tggaagacct gcagcgtcag cggaaacatg
     3121 ccgacatgcg tgtcctccat tttatattct tttccgtgca gaagctgcgc catgtcgatc
     3181 tgacgcggtg ttccgctgtt ccgcggcccc ggtgttctcc gcttccagcc gccgcgcagg
     3241 attcttcccg gtgtctcttt cgcgccctcc agatcacggc aggtctgcac cagcttctga
     3301 tatgccatcg ggctttccgc ctcgccggat ttcagctctg caccaaattc cgcgccgaaa
     3361 tacttccgca caatgtcgag catctcacac gtctggaaca gctcgttgct gcccgcattc
     3421 gtcacaatca ccacgccggt atccggataa ccgagcacgt tctgccccag catgccattg
     3481 aacgcaaagc tgcccggccg ctcctccatc cagacctggt aaccatagcc atattttccc
     3541 atcttttccg gcggtgtcgc atgaacggag gtggacgctg cgacccattc cgccgggacg
     3601 atctgttttc cttcgaattt tccgccgttc acgtacatca ccccgagctt ggccgcgtcc
     3661 tccgggcaca ggaagaggcc ccagccgcct ttcgtaatcc ctgccgggca gctctcccag
     3721 aaaactttcc ggatgccgag aggctcaaac agacgcggcc gcaggtaatc taccattttc
     3781 atgccggtgc gctcctgtat gattgcagac agcagatacg tgttcatact gttgtactca
     3841 aacgcttcgc ccggcgttcc cttgaccggc gcatttaaga agctgtccac ccagtcgttg
     3901 ccggaaacaa cgcccatctc gttgaactgt acgccgctct tcatcgtcag aagatgctcc
     3961 acggtcagat tcgggcgcag gatcttctgc agcagaccgt ttcgtttttc aaaaatatca
     4021 tagacgttct cgtccaggga aagttttcct tcactgatca gaaagccgac cgccatgccg
     4081 gtgatgctct tgcacatcga atacgtcgcg tgccagatgc cgctgcggta tggggcgaaa
     4141 ttgcattcgc agatgacatg tccattccgc aggaccagca gatgatgcat gtccgtatgg
     4201 cgcgcagcgg cgagctcacg aagcatctgc gtcagctttt ccgatgagat gccctcgctc
     4261 tccggcacca cgcgcggaaa atacgtttcc ggagatgcaa aatccccgaa cgcttccttc
     4321 tgcggcgcaa agtccgtctt tc
//
LOCUS       DAOMUF010000173         4286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5812_length_4286_cov_4.398015, whole genome shotgun sequence.
ACCESSION   DAOMUF010000173 DAOMUF010000000
VERSION     DAOMUF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_4286_cov_4.398015"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..469)
                     /locus_tag="PLN77_08875"
     CDS             complement(212..469)
                     /locus_tag="PLN77_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRM44415.1"
                     /translation="MEQGNFTYILECSDGSFYTGWTNNLERRVKAHNAGKGAKYTKSR
                     KPVKLVYFESFSTKQEAMRREWEIKQLSRVEKWKLIQAKKQ"
     gene            complement(642..1517)
                     /locus_tag="PLN77_08880"
     CDS             complement(642..1517)
                     /locus_tag="PLN77_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM44416.1"
                     /translation="MRLGALEAGGTKMVCAIGNANGEIYERISIPTETPEITVPKMIE
                     YFKEKDIQALGIGCFGPIDLNRKSETYGYITTTPKLAWKNYNIVGAFADALDIPVGFD
                     TDVNGSALGEATWGITKGLDNSIYITIGTGVGMGIISNGKLLHGMLHPEGGHVLLTRH
                     PKDTYGGKCPYHSNCLEGLAAGPAIEARWGKKGIELADKKEVWELEAYYIGQALVDCI
                     MLLSPQRIVLGGGVMHQEHMMPLVREEVKKQLNGYLVTKELEDLDSYIVLPSLNDNQG
                     IMGALKLAVDELAMQ"
     gene            complement(1535..2470)
                     /gene="manA"
                     /locus_tag="PLN77_08885"
     CDS             complement(1535..2470)
                     /gene="manA"
                     /locus_tag="PLN77_08885"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HRM44417.1"
                     /translation="MELLFMDPVFKEAIWGGTKLRDVFDYEIPSDTTGECWAISGHAN
                     GMSRVAGGRFDGKTLGELWKEEPEIFGNYPGSQVPLLIKIIDAKNDLSIQVHPDDAYA
                     GEHENGSLGKTECWYVLDCEPGTEIVIGHNAKDKAEVEKMIRNHEWSDFIRKVPVHKG
                     DFFQINPGCVHAIKGGTLILETQQSSDITYRVYDYDRLQNGKPRQLHVEQSIDVIEAP
                     FKTADIDTFTEEIPGAVHTHFVTCKFYSVDKYDIDGVFTLDAKKYYTNVSVIAGEGTV
                     NGIPVKKGTHFIIPANFNGCRFEGKLSVICSNPEA"
     gene            complement(2813..3766)
                     /locus_tag="PLN77_08890"
     CDS             complement(2813..3766)
                     /locus_tag="PLN77_08890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRM44418.1"
                     /translation="MKRDKLRIGVITAALAMMIAGGVLTWQQDARNGKADQAKQGETA
                     QLTEDESTGTTEQQTEETAGDADGTRAVQDQADDVENSLEQVPEMEAACEEEAVGNPE
                     ADAETAQNTPEESDGNTERQDAQAVNGTAADQNGETGSGETAANEETSAASGTVDSAE
                     NQETAVETAGAAQDLMFSEATIMELPVTGTVLIPYNMENTVYFPTLDLYQCNPGVVFS
                     AAEGTPVLAAADGEVVSVENDARTGLTVTMNLGNGYEAIYGQLTDVSLEAGQKVQKAA
                     RIGAVAAPTKYYVKEGSNLFFEMKKDGKAIDPMVYLPAISE"
BASE COUNT          925 a         1153 c          785 g         1423 t
ORIGIN      
        1 cctgtcagaa acgctgtgta tttcaattac tacttcatgt agtttttatt actacttatc
       61 attgtttctt ttattatagc aggataaaat tatttttcaa gtcaaaatat ctattttctt
      121 ttgaaaatgt ttgacctgct ttgacacaaa aaaacagact gctcgcttac gcataccgtt
      181 gtgcctgttt gagccgtctg tttttgtatt tttattgctt cttcgcttga atcagcttcc
      241 atttttctac ccgcgaaagc tgcttaattt cccattctct ccgcatagcc tcctgttttg
      301 ttgaaaaaga ttcaaaatac accagtttta ccggtttgcg gctctttgta tatttcgctc
      361 cttttccagc attgtgtgcc tttactctgc gttccaaatt attggtccag cctgtataaa
      421 aagatccatc gctgcattcc agaatatatg taaaattccc ctgttccatt tcaaattccg
      481 ttccttctgt tcatctgtat tctatgtaac tttaacactc ggttctttta ggcgcaatta
      541 aaaaaataag aaagagtcct gcttcgcagg aaaattcaac gaagtttcct gtaagcaaaa
      601 aaccgtccgg atttctccga acggttttct catttttatt tttactgcat tgccagctca
      661 tctacagcca gtttcagcgc acccataata ccctgattgt cgttcaggct tggaagtaca
      721 atgtagctgt cgagatcctc aagttctttt gttacaagat atccgttcag ctgtttcttt
      781 acttcttctc ttacgagcgg catcatatgc tcctgatgca taacgcctcc gcccaaaacg
      841 attctctgcg gggagagcag catgatgcag tctacaagtg cctgtccgat atagtaagcc
      901 tcaagctccc atacctcttt tttgtctgcc agctcaatac cttttttgcc ccagcgtgct
      961 tcaatggcag gacctgcagc aaggccttcc aggcagttgc tgtgatacgg gcattttccg
     1021 ccgtaagtat ctttcggatg tctggtcagc agaacgtgtc cgccttccgg atgaagcatg
     1081 ccgtgcaaca gcttgccatt ggaaataatt cccattccaa cgcccgttcc gatggtgatg
     1141 taaatgctgt tgtccagacc ttttgtgatt ccccaggtag cctcacccag tgcagaaccg
     1201 tttacatcgg tatcaaatcc aaccgggatg tccagtgcat ctgcgaaagc gcctacgatg
     1261 ttgtagttct tccatgctaa tttgggagtt gtggtaatgt aaccatatgt ttcggatttt
     1321 ctgttcaggt cgatcggtcc aaagcaaccg attcccagtg cctgaatgtc cttctctttg
     1381 aaatactcaa tcattttcgg aacggtaatt tccggtgtct cggtcgggat agaaattctc
     1441 tcataaattt ctccatttgc gtttccaatt gcgcagacca tctttgttcc gcctgcttct
     1501 aatgctccaa gtctcattgt gtttttctcc tctcttatgc ttctggattt gagcagatta
     1561 cagaaagttt tccttcaaat ctgcatccat taaaattagc cggtataata aagtgagtac
     1621 ctttcttaac cggaataccg tttacagtac cctctccggc gattacactt acattagtat
     1681 aatatttttt cgcatccaag gtgaatacac cgtcgatgtc gtatttatct acagagtaga
     1741 acttgcatgt tacaaaatgg gtatgtaccg cgcccgggat ttcctctgta aaggtatcga
     1801 tatctgccgt tttaaacgga gcctcgataa catcgatact ctgctctaca tgaagttgtc
     1861 tcggtttgcc gttctgcagt ctgtcataat cgtatacacg ataggtgata tcgctgctct
     1921 gctgtgtctc cagaatcagt gtgccgcctt tgatggcatg tacgcatccc ggattgatct
     1981 ggaagaaatc tcccttatgt accggcactt tgcggatgaa atcgctccac tcatgattgc
     2041 ggatcatttt ctccacttct gccttgtctt ttgcattgtg tccgataaca atttctgtac
     2101 ccggttcgca gtccagcacg taccagcact cggtctttcc aagagatcca ttctcatgct
     2161 ctcctgcata agcatcatct ggatgaacct ggatgctcag atcatttttt gcatcaatga
     2221 tcttgatcag cagcggaacc tggcttcccg ggtaatttcc gaaaatttcc ggctcctctt
     2281 tccagagctc acctaatgtt tttccatcaa atcttccgcc tgccacacgg ctcattccgt
     2341 tggcatgtcc gctgatcgcc cagcactcgc cggttgtatc gctcggaatt tcgtaatcaa
     2401 atacgtctct taactttgtt ccaccccaga ttgcttcttt gaacaccggg tccataaata
     2461 aaagctccat atttttctaa tcctccttgt tttcaggatc tgtccactac gtctatttta
     2521 accgataaca gccaaaaacg aaacccctaa aatgacaaac tttcacattt tctgtaaccg
     2581 gttactttcc ttttattccg ccctttttct atatcaaaac aatcctgttt gttatatgta
     2641 aagctgactg ttttcagcca gcctttttta taaaaagcaa tcgaaaaacg gtattttacc
     2701 atccgcggtc tgcgccgcct tttttgtatt caaaacctgt aaaaaagatt cgattgcgaa
     2761 tcctacactt tataatactt tcccgcatcc catttcgtgc aggtcctctt ttttattccg
     2821 aaattgccgg gagatatacc ataggatcaa tggcttttcc atcttttttc atctcaaaga
     2881 aaagattgct tccctctttt acataatatt tcgtcggtgc tgcaactgct ccgatccgtg
     2941 ctgctttctg aactttctgg cctgcctcga gggaaacgtc tgtgagctgt ccgtagatgg
     3001 cttcgtatcc atttccaaga ttcatggtga cggtgaggcc ggttcttgca tcattttcca
     3061 cactcacaac ttctccgtcc gctgccgcca gaaccggagt tccttctgcc gccgagaaaa
     3121 caacgcctgg attgcactgg taaagatcca gtgtcgggaa ataaacggta ttttccatgt
     3181 tatacggaat cagaactgtt ccagtaaccg gcagttccat gattgtcgct tcgctgaaca
     3241 tcagatcctg tgcagcccct gctgtctcaa ctgccgtttc ctgattttct gcagaatcca
     3301 cagttccgga tgctgcactc gtttcttcat tcgccgctgt ttctcctgat cctgtttctc
     3361 cgttctgatc cgccgctgtt ccgtttaccg cctgcgcgtc ctgtctttct gtattcccat
     3421 ctgattcttc cggtgtattc tgagccgtct ctgcatctgc ttctggattt cccaccgcct
     3481 cttcttcaca cgccgcttcc atttccggca cctgttccag actgttttcc acatcatctg
     3541 cctgatcctg tacggcacgc gttccgtctg catctcccgc cgtctcctcc gtctgctgtt
     3601 ctgtagttcc ggtgctttcg tcctctgtaa gctgtgccgt ctctccctgt ttcgcctgat
     3661 ctgccttacc gtttctcgca tcctgctgcc aggtcagcac tccgcctgca atcatcattg
     3721 ccagtgccgc tgtgatcacg ccgattctca acttgtctct tttcatgttc ttaccacctc
     3781 tgctgatatc tttttaagca tttcagaggc gtttttcgat ttctcgctct cctcctgctt
     3841 ttcctacagt aagcagtatt tcccatccgg cgcgatctat acacaaaaac cggatttttc
     3901 ctttctgtta tccacaattt ctcctccgtg ctttttcatt ttgtggaaaa catctgccgt
     3961 ttctgcgcac ttcgtgcaaa acatccccat cattatccac actttatttt tgtcgtgtca
     4021 ggcggatatt cgcaatggta ataggagact tccttcattt ggttaaaaaa gagctggaga
     4081 cgggaaaatt cccagttcca gctctttttt tttgttgtaa tctttttatt gcagtcttcc
     4141 atacaccgta ctcgcatccg caatgctgtc caccgcagat cccgaatccg tcatcgcctc
     4201 tgccgtaacg agtatgtact cttctccccc gtattctgca aagctgacca ggcactgacc
     4261 cgcctcatcg gtaaatccgg tctttc
//
LOCUS       DAOMUF010000174         4275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5836_length_4275_cov_5.035308, whole genome shotgun sequence.
ACCESSION   DAOMUF010000174 DAOMUF010000000
VERSION     DAOMUF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5836_length_4275_cov_5.035308"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PLN77_08895"
     CDS             complement(<1..1224)
                     /locus_tag="PLN77_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM44419.1"
                     /translation="MEIHELQQLLSEMSLQEKIGQMVQLTGAYFDKEAVLTGVVGEQL
                     PPEWIIQYAGSVLGVIGKDKIYDIQSRYMEQHPHHIPLLFMADVIHGCRTIFPIPLGQ
                     ACSFHPELVSEAASIAASEASSEGLRATFSPMIDVSRDPRWGRMMESFGEDPYVNGIM
                     GKAMVDGYQQKDDAGIAACLKHFAGYGAVNAGREYNDVEISKRTFLEQYVKPFRMALK
                     AKPAMVMTAFNAIDRKPISGNKELLKGLLRDKEGFEGTVISDWGSIGQLEEQGVAADM
                     EEAAIQASEAGVDIDMMSPAYMLCLEKLVESGKIPEAFVDESAFRVLMMKNQLGLFEN
                     PFAGLGKSGKLTLHNREKAYQLAGESCVLLKNEKLLPLSRKKKVIWAGPYTASRELLS
                     RWSIFGEHEAVETIED"
     gene            complement(1316..3109)
                     /locus_tag="PLN77_08900"
     CDS             complement(1316..3109)
                     /locus_tag="PLN77_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HRM44420.1"
                     /translation="MKKQYFCNPLNMDYRYQFIQDMRSGEDKISREAADPSMILFQGK
                     YYIFASMTLSVWVSEDMVHWESHRLPDSLPLYDYAPDVRVVGDYVYFSASRRGTICDF
                     YRTKDILNGPFERIPGTFDFWDPNLFLDDDGKLYFYWGCNNVTPIWGVELEPETMVPK
                     TERKELIYGQPEKIGYERVGENHSKMPLSEEEAVEKFKEFLKAQGKDADHLTEEQIGF
                     AKIMFSQRPFIEGAWMTKHDGTYYLQYAGPGTEYNVYGDGVYESDSPLGPFRLAKNNP
                     YSYKPGGFLRGAGHGSTMEDRYGNWWHTATMQISKNHDMERRVGIWRAGFDKDGVLFC
                     NQQFGDWPMAVEQAKEDPWAEPEWYLLSYQKAMTASSSEEGREPSFATDENIQTWWRA
                     AGNQPGEWISMDLGEVKDVRAVQINFADDKIDAPLPGERQGERYIDPSQHKTRWLLEA
                     SADGTNYFVLADKSDAETNLPHDFIVKEEGVQIRYLKLTVFEVPYNQNPCISGLRVFG
                     LGNGEKPSAPQYQAERTGTMDMRITIEPQTDAVGYNVLWGFAPDKLYHSCMTFMPEQN
                     IGALVEGETVYVRVDAFNENGITEGTVRPLA"
     assembly_gap    3365..3374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3446..4078
                     /gene="grpE"
                     /locus_tag="PLN77_08905"
     CDS             3446..4078
                     /gene="grpE"
                     /locus_tag="PLN77_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRM44421.1"
                     /translation="MECGDFTLILQSGEKRAKIDLSQFTEEKPEPFPINTVFVPKQLL
                     ANVLQAGKEFCLGAKNLNPTTKDSAIGKGIQMIARQTQEVFADCHDGDTNIRVSTWIE
                     KLLAVKDNLEYGIVSSERTTNRWAYMDEKLLMMDFRTALAQNLYQLNVLPIEANGAIF
                     DPHIHDAVHIEETDRVEEDRVVEEIQKGYFFGEKLYRPTKVIVSKNPCQK"
BASE COUNT         1104 a         1087 c          879 g         1195 t
ORIGIN      
        1 atcttctatt gtttcaactg cctcatgttc tccaaaaatt gaccagcggc ttaataattc
       61 tcttgacgct gtataaggac ctgcccagat aactttcttc ttcctgctta atggcagcag
      121 cttttcattt ttcaaaagga cacagctttc gcccgccagc tgatatgcct tttcccggtt
      181 atgcaaagtc agttttccac tctttccaag accggcaaac ggattttcaa aaagccccag
      241 ctgatttttc atcatcagga ccctgaatgc actttcatca acaaacgctt ccggaatttt
      301 gccgctttcc accagttttt caagacacag catataagca ggactcatca tatcaatgtc
      361 tacaccggct tctgaagcct ggattgctgc ttcttccata tcagctgcca ctccctgttc
      421 ttccagctga cctatggagc cccaatctga aattacagtt ccctcaaatc cttccttatc
      481 ccgcagaagc cctttaagaa gttccttatt tccgcttatc ggtttacggt caatagcatt
      541 aaaagctgtc atgaccatag caggctttgc ttttaatgcc atacggaaag gttttacata
      601 ctgttccaga aatgtccgct tggatatttc aacatcatta tattcccttc ctgcattcac
      661 tgccccataa cccgcaaaat gcttcaggca tgccgctatt ccggcatcat ctttctgctg
      721 atagccatct accattgctt ttcccatgat cccatttaca tatggatctt ctccaaagga
      781 ttccatcatc cttccccatc tgggatctct tgaaacatca atcataggag aaaatgttgc
      841 tctgagccct tctgaagatg cctccgaggc agctatagaa gccgcctcag acaccagttc
      901 cggatgaaat gaacacgcct gccccaacgg aatcggaaaa atcgtccgac atccatggat
      961 aacatctgcc ataaaaagaa gcggaatatg atggggatgc tgttccatat atctgctctg
     1021 aatatcataa attttatctt taccgatcac tccaagcact gaaccggcat attgaatgat
     1081 ccattccggt ggcaactgtt cgccaacaac acctgtgagc actgcctctt tatcgaagta
     1141 tgcgccagtg agctgcacca tctgtcctat tttttcctgt aatgacattt ctgacagcaa
     1201 ttgctgtaat tcatgtattt ccataaatac tccttatgtt caatccgttt atttctaatg
     1261 ttcatttctc caaagagcac ctgagctgtt tttctgcttt ttcaatataa ttgtatcatg
     1321 caagcggcct tacggttcct tccgtaattc cattctcatt aaacgcatcg actctcacat
     1381 acaccgtttc gccttctacc agcgctccaa tattttgctc cggcataaag gtcatgcagc
     1441 tgtgatacag cttgtccggt gcaaaacccc agaggacgtt gtatccgaca gcgtctgtct
     1501 gtggctcgat ggtgatgcgc atatccatag ttccggtccg ctctgcctgg tactgcggtg
     1561 cggatggttt ttcgccgttg ccaaggccga aaacacgaag gccggagatg catggattct
     1621 ggttgtacgg cacttcaaaa acagtgagtt tcagataacg gatctgtaca ccctcttctt
     1681 ttacgatgaa atcgtgcggc aaattggttt ctgcgtctga tttatctgcc agaacaaaat
     1741 agtttgttcc atcggcggat gcctcaagca gccatctggt cttatgctgg ctcgggtcga
     1801 tatatcgttc cccctgacgc tcgcccggca gaggtgcatc gattttgtca tctgcaaaat
     1861 tgatctgtac ggcgcggaca tcttttacct caccaagatc catcgaaatc cactcacccg
     1921 gctgatttcc ggctgcgcgc caccaggtct ggatattttc atctgtcgca aacgacggtt
     1981 ctctgccttc ttccgaggac gatgccgtca ttgctttctg atagcttaag agataccact
     2041 ccggttctgc ccacgggtct tcttttgcct gctccaccgc catcggccag tcgccgaact
     2101 gctgattgca gaacaggaca ccatctttgt cgaagcctgc ccgccagatg ccgacgcgac
     2161 gctccatatc gtggtttttg ctgatctgca ttgtcgcggt atgccaccag tttccatagc
     2221 ggtcttccat cgtcgaaccg tggcctgcac cgcgcaggaa tccgcccggt ttataggaat
     2281 atggattgtt ctttgccaga cggaacgggc caagcgggga atcactctcg taaacaccgt
     2341 caccgtaaac gttgtattcc gttcccgggc cggcatactg gaggtaatag gttccgtcat
     2401 gcttcgtcat ccatgcgccc tcgatgaacg gtctctgact gaacataatt ttggcaaacc
     2461 cgatctgctc ctccgtcaga tgatccgcat cttttccctg tgctttcaga aattctttga
     2521 atttttcaac agcttcttct tcactcagcg gcattttgga atgattttct ccgacgcgct
     2581 cgtatccgat tttttccggc tgaccgtaga tcagttcttt tctctcggtc tttggaacca
     2641 tcgtctccgg ttcaagctcc acaccccaga tcggagtcac gttattgcat ccccagtaaa
     2701 aatagagctt tccgtcatca tcaagaaaca ggttcggatc ccagaaatcg aaggttcccg
     2761 gaatacgctc aaacggcccg ttcaggatat ctttcgtgcg gtaaaaatca cagatcgtgc
     2821 cgcggcggga tgcgctgaaa tagacgtaat cgccgaccac gcgcacatcc ggcgcatagt
     2881 catacagcgg caggctgtcc ggcagacggt ggctctccca gtgaaccata tcctccgaaa
     2941 cccagacgct caacgtcatg gatgcaaaaa tataatattt tccctgaaat aaaatcatcg
     3001 acggatcggc ggcctcgcgg gaaatcttgt cctcaccgct tctcatatcc tgaatgaact
     3061 ggtaacggta atccatattt aacggattgc agaaatactg ttttttcatc aaaattcctc
     3121 tttccggacg gtacgtaaaa acccctgttc gtacatcctt ttttcttatg atagaaaaag
     3181 tttagcatat cccgcaaaga aaggaaatgt tctgatttga cttttttgtt gcactttttg
     3241 gactgttttt tgtctcaggt attggacaaa ttcctgtgtt ggatttataa ttcgtgtaat
     3301 ttcaaattgc agaaaacaag atctgcaagc cgagttttca gcatggatcg aggttgaaat
     3361 ggaannnnnn nnnnagaaaa tggaggagat ctgtcctgaa tttccaaagg atgcaagccg
     3421 gctgaaagca tggatcgagg ttggaatgga atgcggcgat tttaccctga tactgcaatc
     3481 aggagagaaa cgtgcaaaga tagatttgag tcaattcaca gaagaaaaac cggaaccttt
     3541 cccgataaac accgttttcg taccgaaaca gcttctcgca aatgtacttc aggccggaaa
     3601 agagttttgc ctgggtgcaa aaaatctgaa ccccacaacg aaggattctg caattggaaa
     3661 gggaatccag atgatcgcca gacagacaca agaagtattt gcagactgtc atgacggaga
     3721 tacaaacatc cgggtcagca catggattga aaagctgctg gctgtaaaag acaatcttga
     3781 atatggaatt gtcagttcgg aaagaacgac aaatcgctgg gcctatatgg atgaaaaact
     3841 tcttatgatg gatttccgta cagctttggc gcagaattta taccagctga atgtcttacc
     3901 gattgaagca aatggtgcaa tatttgatcc tcatatccat gatgcagtac acatcgaaga
     3961 gacagaccgg gtggaagaag acagagttgt cgaggaaata cagaaaggct acttcttcgg
     4021 tgaaaaactg tatcgaccga caaaagttat tgtttctaaa aatccctgtc aaaagtaatg
     4081 ctctgaatgc acttcaaaag caccggccaa atggtcggtg cttaaataat ggtttcatca
     4141 tcctgtcaaa aataatgctc tgccatgtgt gtcttgatat atacgaatca atttctcaaa
     4201 cgcacagatc attaaccgat ataatcgagt aggagaaaat tcctcttgat gaggaatttt
     4261 cgacctctca cacca
//
LOCUS       DAOMUF010000175         4266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5850_length_4266_cov_3.645452, whole genome shotgun sequence.
ACCESSION   DAOMUF010000175 DAOMUF010000000
VERSION     DAOMUF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5850_length_4266_cov_3.645452"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLN77_08910"
     CDS             complement(<1..265)
                     /locus_tag="PLN77_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44422.1"
                     /translation="MVELLLLAAALSADNFAVSIGYGSSNTRISPGMTALLNLFCSAA
                     LVASAQFGKRFLQSAGPEWGTRVGAGCFFLMGTLKIFEELRKRR"
     gene            complement(346..1017)
                     /locus_tag="PLN77_08915"
     CDS             complement(346..1017)
                     /locus_tag="PLN77_08915"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM44423.1"
                     /translation="MRKSQISASMMCSNLVDLQATIDVFEKEKVEYLHIDVMDGEFVP
                     NFGLGVDYIRGLRELTSIPLDLHLMIKDPEYKLPWIGIHKEDIVTIHYESTFQVQRAL
                     DYLVPYGCKRFLAINPATPIGQIEEVLDYIDGVNLLMVNPGFAGQKIVPSTLRKAEKL
                     QKFLQEMHREDIILEVDGNITKEHGATLRSCGASIFVAGTSSIFCTDVSHFGEKIREF
                     RKAVE"
     gene            complement(1024..2037)
                     /locus_tag="PLN77_08920"
     CDS             complement(1024..2037)
                     /locus_tag="PLN77_08920"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HRM44424.1"
                     /translation="MRAYELHGVNDLRREDIEKPEIPSGWVLVQVKASGICSSDIPRI
                     FTNGTYHFPTIPGHEFSGVVAAYGEGVPEERVGKRVGIFPLIPCRTCPQCRQKKYEMC
                     EHYDYLGSRRDGGFAEYVAVPDWNLMELPENVSFREAAMLEPLSVALHAVKRSGVKPG
                     DTAAVIGTGMIGFAAAAWAKALGAETVFVIGRGEAKRAIAEQIPGISYVTEEESKAIQ
                     ADVVVEAVGTPQAVSSAVLLARPGGSIVLMGNPSGDLAMEKNVYWRILRKQLNLMGTW
                     NSSYEKDEACDWTEALDALSKKKIPAQALITHCFPSEKLMDGLELMKNHKEPYCKVMV
                     SWE"
     gene            complement(2034..2738)
                     /locus_tag="PLN77_08925"
     CDS             complement(2034..2738)
                     /locus_tag="PLN77_08925"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HRM44425.1"
                     /translation="MNYAIILSGGIGTRMQMGDFPKQYLEVEKKPILLYTLEQFQKSS
                     AVEKIVIVAADAWREKIRGWMEEDGITKFLAFADAGDTRQESIHNGLTVCMEDSVSEN
                     DGVIIHDGVRPLVSEQLIGDCLAALADHEGCMPVLPMKDTIYQSSDGTKIDHLLERST
                     LFAGQAPEAFRLHPYAKINREASKEELSLTRGTSEIAYRHGMDVAMIPGDERNFKITT
                     RSDLERFCTIVEGETK"
     gene            complement(2740..>4266)
                     /locus_tag="PLN77_08930"
     CDS             complement(2740..>4266)
                     /locus_tag="PLN77_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRM44426.1"
                     /translation="STVEGVGHFEPLRHYAEVHLLLEPAEPGSGLQIGTVCSEDTLDR
                     NWQRLILTHLLERKHPGVLTGSEITDMKITLVKGRAHIKHTEGGDFRQATYRAVRQGL
                     KKAESVLLEPVYAFRLEIPSESTGRALNDIQRMYGSFEPPEMEGDMTVITGTAPVVTM
                     RDYQKEVTAYSRGRGRVFCTLKGYEPCHNAEEVIASIGYDSEADVENPTGSVFCAHGA
                     GFVVPWNEVEDHMHLEYTLENPEEESDSAESAADRSGGASSVQKAKKASDRVPMAASL
                     QEAKELEEIFTRTYGKVERKRAGFERRTRPVTSSSGIGDPKYAKSNRPKEPQEEYLLV
                     DGYNIIFAWDDLNELAKYNIESARGKLMDILSNYQGYKKMTLILVFDAYKVKGNQGEV
                     GMYHNIHVVYTKEAETADQYIEKTVHRIGHNGNVTVASSDGLEQIIIMGAGAHRLSAR
                     DLRTEIEHTNGQIRENYLEKEQKTKSYLLENASGELGDFLKELEEERKKESKKSKGKA
                     "
BASE COUNT          850 a         1307 c          975 g         1134 t
ORIGIN      
        1 ttctgcgttt tctcagttct tcaaaaattt tcagcgttcc cataagaaaa aagcagcccg
       61 cacccactct ggttccccat tccggtcctg cggactgcag aaatcttttt ccaaactgcg
      121 cggaagcaac cagggcagca ctgcaaaaca gattcagcag cgccgtcatg ccgggtgaaa
      181 ttctcgtatt gctgcttcca tatccgatgc ttactgcaaa gttatcggct gagagcgccg
      241 cagcaagcag aagcaattct accatatgac acgctccgtc atttctcact ttacagtatt
      301 cgtctgtttt tcaaaccgtg catcccggga aggcagttct ttttcttatt ccactgcttt
      361 ccggaattcc cggatttttt ctccgaagtg cgatacatcg gtgcagaaaa tgctcgaagt
      421 tcccgccaca aaaatgctgg ctccacagga acgcagcgtc gcgccgtgct ccttcgtaat
      481 gtttccgtcc acttctaaaa tgatgtcctc ccggtgcatc tcctgcagaa atttctgcag
      541 cttttccgcc ttgcgcagcg tactcggcac aattttctgc ccggcaaagc ccggattcac
      601 catcagaaga ttgacgccgt cgatataatc aagcacttcc tcgatctgcc cgatcggtgt
      661 cgccgggttg atcgcgagga accgcttgca gccgtacggc acgaggtaat cgagcgcgcg
      721 ctgcacctgg aaagtgctct cataatgaat cgtcacgatg tcctctttgt gaatgccgat
      781 ccacggcagc ttgtactccg ggtctttaat catcagatga agatcgagcg gaatactcgt
      841 cagttcccgc agtccgcgga tgtagtcgac gccaaggccg aagttcggaa cgaactcgcc
      901 gtccatcaca tcgatatgga gatattcgac tttttctttt tcaaatacat cgatcgtcgc
      961 ctgcagatcc acaaggtttg agcacatcat ggatgccgat atctgacttt ttctcatatt
     1021 tttctactcc catgaaacca ttactttgca gtatggctct ttatgatttt tcatcagttc
     1081 cagtccgtcc atcagctttt cggacgggaa acagtgcgtg atcagcgcct gtgccgggat
     1141 tttcttcttc gaaagcgcat cgagtgcctc cgtccagtcg caggcttcat ctttttcata
     1201 agaagaattc cacgttccca tcaggttcag ctgtttgcgc aggatgcgcc agtacacgtt
     1261 cttttccatc gcaagatcgc ccgatggatt tcccatcagc acgatgcttc cgcccggtct
     1321 cgcaagaaga actgcactgg aaacggcctg cggcgttccg accgcttcta ctacaacatc
     1381 cgcctgaatc gctttactct cctcttccgt cacatatgag atgcccggga tctgttctgc
     1441 gatcgcacgt tttgcttcgc cccgtccaat cacaaaaacg gtttcggcgc cgagcgcttt
     1501 cgcccacgct gccgctgcaa aaccgatcat gcccgttccg atcactgctg cggtatcgcc
     1561 cggcttcaca ccgcttcgtt tcaccgcatg aagcgccaca gagagcggct ccagcattgc
     1621 tgcctcgcgg aagctgacgt tttccggcag ctccatcagg ttccagtccg gcacggccac
     1681 gtattccgca aagccgccgt cccggcgcga gccgaggtaa tcgtagtgtt cgcacatctc
     1741 atattttttc tgccggcact gcgggcaggt gcggcatggg atcagcggga aaatgccgac
     1801 ccgttttcca actcgctctt ccggcacacc ctcgccgtat gcggcaacca cgcctgaaaa
     1861 ctcgtgaccc ggaatcgttg gaaaatggta tgttccgttt gtgaaaatcc gcggaatatc
     1921 ggagctgcag attcctgatg cctttacctg caccagaacc cagccggatg ggatttccgg
     1981 tttttcgatg tcttcccttc ttaaatcatt gactccatgc agctcgtatg ctctcatttc
     2041 gtttctcctt ccacaatcgt acagaaacgc tccaggtcgc tccttgttgt aatcttaaag
     2101 ttccgctcat cccctgggat catcgccaca tccattccat ggcggtaggc aatttcactc
     2161 gttccccttg tgagggacag ctcctctttc gatgcttccc ggttgatctt cgcgtacgga
     2221 tgcagccgga aggcttccgg cgcctgaccg gcaaacagcg tgctgcgctc cagcagatgg
     2281 tcaattttcg ttccgtcgct gctctgatag atcgtgtcct tcatcggcag caccggcata
     2341 cagccctcat gatcggcaag cgccgcaagg cagtcgccaa tcagctgttc gctcaccaga
     2401 ggccgcacgc cgtcgtggat gatcacaccg tcgttctccg atacggaatc ctccatgcag
     2461 acggtaaggc cattatgaat ggattcctgc cgcgtatccc cggcatccgc aaaggcgaga
     2521 aatttcgtaa tcccgtcttc ttccatccat ccgcggatct tctctctcca ggcatctgcc
     2581 gccacgatga cgattttctc aacggcactg cttttctgaa actgctccag cgtatacagc
     2641 agaatcggtt tcttttccac ttccagatac tgcttcggaa aatcccccat ctgcattctc
     2701 gttccgattc cccccgatag tatgattgcg taattcatct taagcctttc ctttgctctt
     2761 tttactctct ttttttcgct cttcttccag ctcttttaaa aagtctccca gttctccgga
     2821 ggcgttctcc agcaggtacg acttcgtttt ctgttctttc tccagatagt tttcccggat
     2881 ctggccgttt gtatgttcaa tctcggtgcg cagatcccgt gcggagagac gatgcgcgcc
     2941 cgctcccata atgatgatct gctcgagacc gtccgaggaa gccaccgtta cgttgccgtt
     3001 gtggccgatc ctgtgaaccg ttttctcgat gtactggtca gccgtctccg cctctttcgt
     3061 ataaacgaca tgaatattat ggtacatgcc gacctcaccc tggttgcctt ttactttgta
     3121 ggcatcaaag acaagaatca gcgtcatttt tttatatccc tgataatttg acaggatgtc
     3181 catcagtttt cctctggcgc tctcaatatt atacttcgcc agctcattta aatcatccca
     3241 tgcaaaaata atgttatagc cgtccaccag cagatattcc tcctgcggct ccttcgggcg
     3301 gtttgatttt gcatatttgg gatccccgat gccggatgac gaggtgacag gacgtgtccg
     3361 ccgctcgaaa ccggcgcgct tccgctccac tttgccgtat gtcctcgtaa agatttcttc
     3421 cagctccttt gcctcctgca gggaagctgc catcggcacg cggtcggatg ctttttttgc
     3481 tttctgcaca gaggatgctc cgccgctccg gtctgccgca gattcggctg aatcgctttc
     3541 ttcttccgga ttttccagcg tgtattccag atgcatatgg tcttccactt cattccatgg
     3601 aacgacaaag cctgccccgt gggcacagaa cacagaaccc gtcggatttt ccacatccgc
     3661 ctcggaatcg tagccgatgg aggcaatcac ttcctccgcg ttgtgacacg gctcatatcc
     3721 ttttaaggta cagaacactc ttccgcgtcc ccggctgtaa gcagtcactt ctttctgata
     3781 atcccgcatc gtcacaaccg gtgcggttcc ggtgatcact gtcatatcgc cttccatttc
     3841 cggcggttca aagctgccgt acatccgctg gatatcgttc agcgcccgtc ccgtcgattc
     3901 cgacggaatt tccagacgga acgcatagac cggctccagc agaacggatt ctgctttttt
     3961 cagcccctgc cgcaccgcac gatacgtcgc ctggcggaag tcgccgccct cggtgtgctt
     4021 aatgtgcgca cggcccttga ccagcgtgat tttcatatcg gtaatctccg acccggtcag
     4081 cacgccggga tgcttccgct ccagcagatg tgtgaggatc agacgctgcc agttgcggtc
     4141 cagggtgtcc tcgctgcaga cggttccaat ctgcagtccg ctgcccggtt ctgccggctc
     4201 cagcagcaaa tgaacttccg cataatggcg cagcggctcg aaatggccga caccctccac
     4261 ggtact
//
LOCUS       DAOMUF010000176         4166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_5997_length_4166_cov_5.073948, whole genome shotgun sequence.
ACCESSION   DAOMUF010000176 DAOMUF010000000
VERSION     DAOMUF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5997_length_4166_cov_5.073948"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..769)
                     /locus_tag="PLN77_08935"
     CDS             complement(59..769)
                     /locus_tag="PLN77_08935"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM44427.1"
                     /translation="MYQNYIFDLYGTLVDIHTNEKKAYLWKKMSLFFGMKGAAYEPKE
                     LRMAYETKIKEQEAALKMECRGSHVPEIDIADVFRQLFEDQAVSVTEKEIADLAKMFR
                     AISIEELKLFPGVPEMLQRLKDAGKKVFLLSNAQALFTAPEISLLGLTKYFDGILLSS
                     DAGVKKPDPAFYEMLLKQYHLDPAECLMTGNDDIADCHGAASAGIASRYVATRQSPKI
                     NDPLPENCEKIAAIAELF"
     gene            complement(1081..1965)
                     /locus_tag="PLN77_08940"
     CDS             complement(1081..1965)
                     /locus_tag="PLN77_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRM44428.1"
                     /translation="MTREEFEQLAGAGVILLDGATGSNLTKAGMPKGVSTELWVLEHP
                     DILANLQKAYVEAGSQIVYAPTFGANRLKLADYGRENEVEELNARLVDISKKALAGTG
                     ALIAGDFSTPGKMLQPKGDLSYEELLESYTEQVTAVANAGVDLLVAETMLSVDETCVF
                     MDAALSVCDLPIMCSLTLEADGSALFGGNAVEAVETLQEMGASAVGLNCSVGPDQLEA
                     VVNSMKKVARVPVIVKPNAGLPKITPTGEAIYSMDAPTFAKYMNILVDAGAGIVGGCC
                     GTTPEYIRLVKEMLDARK"
     gene            complement(2716..>4166)
                     /locus_tag="PLN77_08945"
     CDS             complement(2716..>4166)
                     /locus_tag="PLN77_08945"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRM44429.1"
                     /translation="FLSIHFALHEEEVMKNLKAKAVCVMLSASMAAMGLTGCGSDNVD
                     GTKTAITVNDESITLGQANFMLRYQQATMYNYYSKMYSMYGMQMPSEMYDKEGDDGKT
                     TGETFKEQSLDTIEKELLMRQHAEEYGISLTDEEKQQAKEAAQAFADKNGDDVMKKLH
                     ATVEDIQDALELYVIQTKIYDPIIADVDTEVSDEEAKQTSISYITVSTAGTEKDDDGK
                     TIDLTDEEKAAKKEIAQRFLDLLKESEDPAAASFTDLRKELNDQLNAENTADSTDSAD
                     SSDESSSSSDASDTSASDASSASTSSSSASDSSSEVSYLTSSETSFGTGSEKDDDDTC
                     SLGDKVAEEAAKLKDGEYYDGVIEGDDAYYVIRVDKAFDEDKTESRRQTIISNRKSDK
                     YNDTLDGWVKESDIKVASNWKKLEVTDADLYTMTVDSASTDSTDGTTTDSTTTDSTAT
                     DSTTSDSTTSGSTETTSTSSTGTASTSSSTAE"
BASE COUNT          884 a         1128 c          863 g         1291 t
ORIGIN      
        1 gtgcgacagc cccttttttc gcattatttt ttcaggagtg ctgctgaatg ctcttcagtt
       61 aaaataactc cgcaatcgcc gcgatcttct cacagttttc cggcagcggg tcgtttattt
      121 tcggtgactg gcgcgttgcc acgtaacggc ttgcaattcc ggcagaggct gcaccgtggc
      181 agtcagcgat atcgtcattt cctgtcatca ggcattctgc cggatcgagg tgatactgct
      241 tcagcagcat ctcataaaac gccggatccg gttttttcac accggcatcc gatgagagca
      301 ggattccatc aaagtatttc gtaaggccga gcagggaaat ttccggcgcg gtgaaaagcg
      361 cctgtgcgtt ggacagcaga aaaaccttct ttcctgcgtc ttttaaacgc tgcagcattt
      421 ccggcacgcc ggggaacagt ttcagttctt ctatcgaaat cgcacggaac attttcgcca
      481 gatctgcaat ttctttttcc gttaccgaaa cagcttgatc ctcaaacagc tgccggaaca
      541 catccgcaat gtcgatttcc ggtacatggc tgccgcggca ctccatttta agcgcagctt
      601 cctgctcttt gatttttgtc tcatacgcca tccggagttc tttcggctca taggcggccc
      661 ctttcatccc aaagaacagg ctcatttttt tccagagata cgctttcttt tcattcgtat
      721 gtatatctac cagagttcca tataaatcaa aaatataatt ctgatacatc cttcatcatc
      781 ctttcttacg gaaagttttc cacacgacac gtattctacc agacttttct ccacaattcc
      841 atgtcttttt tccagacttc ctatcctttt ttctccaccc agattgtcta tcaaaccaga
      901 tcgttgaaac ctgttttgtc ttgttctctg taagaaagaa tcctgcccgg agggcttttt
      961 acgttatcgg aaaattcaac aaagtttccg gtaacgtaaa aaaattcccc cgtacagatt
     1021 ttgtccgcaa caggggaatt cattacatct cacagtttcc tgcgaattgt aatctttgat
     1081 ttactttctc gcatccagca tttctttcac cagacgaata tactccggtg tcgttccgca
     1141 gcagccgccg acaatgcctg ctcctgcgtc taccagaatg ttcatatatt ttgcgaacgt
     1201 cggcgcatcc atgctgtaga ttgcctctcc ggtcggcgtg attttcggca ggccggcatt
     1261 cggttttacg ataaccggta ctcttgccac ttttttcatg gaatttacga ctgcctcaag
     1321 ctgatccggt ccgaccgagc agttcagtcc tacggcggat gctcccattt cctgcagtgt
     1381 ctcaactgct tccacagcgt ttccgccgaa cagggcagaa ccgtctgctt ccagcgtcag
     1441 agaacacata atcggcagat cgcagacaga aagtgcagca tccataaaga cacacgtttc
     1501 gtctacgctc agcatcgtct ccgctaccag aagatcaacg cccgcatttg ctaccgctgt
     1561 tacctgctct gtgtaggatt ccagcagctc ttcataagag agatcgcctt ttggctgcag
     1621 catttttcct ggtgtggaaa aatctcccgc gatcagcgca cctgttccgg caagcgcttt
     1681 tttcgagatg tccaccaggc gtgcattcag ctcctccacc tcgttttctc ttccataatc
     1741 cgctaatttc aatctgtttg caccaaaggt cggcgcatag acaatctggc ttcccgcctc
     1801 cacgtaagcc ttctgtaaat ttgccagaat atcagggtgc tccagaaccc aaagctctgt
     1861 actgactcct tttggcattc ccgcttttgt cagatttgat ccggttgcgc catccaataa
     1921 aatgacacct gctccggcaa gctgttcaaa ttcttctctt gtcattttct tttctccatt
     1981 ctgcccgagt tacctgcagc acatgtccgc gtatactgac aaatcccggc tgttttttct
     2041 gaacctgccg cgcttttcgc gtcaaattca tgttcagtat agcattgtat tgttttcccg
     2101 acaagtaaaa aaagaggtat ccgttttgga taccccttcg tctgtttctg ttcatgaatg
     2161 ttcagcaaac cccgacattc tcaatcatga tgcgcgtctg aatgatgtgt ctccgaatga
     2221 tgcgtctccg aatgatgtgt cctgcccgag ctgttccccc caccggacgg gatattcttc
     2281 ggtgaccccg gagacaaatt tgttccctgg ttcacacggg ttgctttctg tccattttcc
     2341 acctgcgagg atgccgatac cgttgaaaca cccgaatcgg aactgttttc cgttgcagca
     2401 ttttttccac acgcaacgaa agccaatact gctgtaccga caagtacgga aattactgcc
     2461 ttcttcattt ctccaccttc tttcctggta catcgttttc gcctgaacat tagagcgtgt
     2521 ccgaaaaatt atttccgcaa tttgcgagat acactctggt tgtaatttca ttttaaataa
     2581 gcttcttact attgtcaatt tattctacaa attccctatt attttctttt ttttgttcca
     2641 aaatattttc agacacaaac agcagccgga cccctctccc ggatccggct gccgtttgtc
     2701 ctgtatcgta tgcttttatt ctgcagtgct tgaagacgtc gaagcagtgc ctgtgctgct
     2761 tgtcgatgtc gtttcggtac tgcctgatgt cgtgctgtca gacgttgtac tgtccgttgc
     2821 ggtactgtcc gttgttgtac tgtcggttgt ggttccatct gtggaatctg tagaagcgct
     2881 gtcaactgtc atcgtataca gatctgcgtc cgttacttca agttttttcc agttggatgc
     2941 gaccttgata tcgctctcct ttacccatcc atccagtgta tcgttgtact tatccgattt
     3001 gcggttggaa atgatagtct gtcttctgga ctcggttttg tcctcgtcga atgctttatc
     3061 tacacggatc acataatatg catcgtcgcc ttcgatgaca ccgtcataat actcgccatc
     3121 cttcagcttt gctgcttctt ccgctacctt atctccaaga gaacaggtat cgtcatcgtc
     3181 tttctccgat ccggttccaa aggatgtctc ggaagaggtc agataagaaa cctcagagga
     3241 ggaatcgctg gcggaactgc tcgatgtgct cgcgcttgaa gcgtcggatg cgcttgtgtc
     3301 ggaagcatcg gaagaagagc tgctctcatc ggagctgtcg gcagaatcgg tggaatctgc
     3361 ggtattctcc gcatttaact gatcgttcag ctccttgcgc agatcggtaa acgatgcggc
     3421 tgcaggatcc tcagactctt ttaagagatc caggaatctc tgtgcgattt ctttctttgc
     3481 cgctttttcc tcgtccgtca aatcaatcgt ttttccatcg tcatccttct cggttcctgc
     3541 tgtggatacc gtgatgtagc tgatggaggt ctgctttgcc tcttcatcgg atacctcggt
     3601 atctacatct gcgatgatcg gatcataaat tttggtttga ataacataca gctccagtgc
     3661 atcctggatg tcctcaacgg ttgcgtgcag ctttttcatc acatcgtcac cgttcttatc
     3721 tgcgaatgcc tgtgcagcct ctttcgcctg ctgtttttcc tcgtctgtca gggaaattcc
     3781 gtattcctct gcgtgctgcc gcatcagaag ctctttttcg atcgtatcaa gagactgctc
     3841 cttgaacgtt tccccggtag tctttccatc gtcgccttcc ttgtcgtaca tctcagacgg
     3901 catctgcatt ccgtacatcg agtacatctt ggaataataa ttgtacatgg ttgcctgctg
     3961 atatctcagc atgaaatttg cctgtccgag tgtaatcgac tcgtcattta cagtaattgc
     4021 cgtttttgtt ccgtccacat tgtcggaacc gcagccagtc agtcccatcg ctgccatcga
     4081 agcactcagc ataacgcaga ctgcttttgc ttttaaattt ttcattactt cctcctcatg
     4141 cagggcgaaa tgtatgctca aaaaga
//
LOCUS       DAOMUF010000177         4144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6036_length_4144_cov_3.655662, whole genome shotgun sequence.
ACCESSION   DAOMUF010000177 DAOMUF010000000
VERSION     DAOMUF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6036_length_4144_cov_3.655662"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..167
                     /locus_tag="PLN77_08950"
     tRNA            94..167
                     /locus_tag="PLN77_08950"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Met,seq:cat)
     gene            357..1490
                     /gene="rffA"
                     /locus_tag="PLN77_08955"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             357..1490
                     /gene="rffA"
                     /locus_tag="PLN77_08955"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HRM44430.1"
                     /translation="MIEFNKPPFIGTELEYIRQAAEENNKLCGDGPFTKKCSAWMQDN
                     FNVKHAMLTTSCTHALEMAAYLSEIQPGDEVIMPSYTFVSTADAFVLRGAKIVYVDIR
                     PDTMNIDETKIEDAITEKTKAIVPVHYAGVACEMDTIMDIAKRHNLKVVEDAAQGVSA
                     YYKGKALGTIGDFGCYSFHETKNYTMGEGGALLFQDENYLEKAEILREKGTNRSKFFR
                     GQIDKYTWIDYGSSYLPSDMNAAYLLAELEEHEKIDRKRMAIYNYYHEQLRPLAEAGK
                     IEQPVVPEGCVHNAHMYFIKARNLEVRTKLIKYMKERGVMCVFHYVPLHTSPAGQKFG
                     VFHGEDKYTTKESERLMRLPMFYSLSEQDMAYVVECLMDFKEW"
     gene            1497..2105
                     /locus_tag="PLN77_08960"
     CDS             1497..2105
                     /locus_tag="PLN77_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRM44431.1"
                     /translation="MYEKFLNYFTDYIFVKDEPQKADVIFVPGNGYPQMAAHAAELWK
                     EGFAPYVLPSGRYSTVLGHFAGVQDLAEQYGGNYETEWEFLRTVLQKNGVPEKAILRE
                     DQATYTYENALRSREVTDAAGIEVKKAILCCRNLHARRCILYYQLVYPETEFFVCPSD
                     IEFTRENWHTKGEWIDQVLGEMERCGGQFHKILRELPADSEF"
     gene            2386..2616
                     /locus_tag="PLN77_08965"
     CDS             2386..2616
                     /locus_tag="PLN77_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44432.1"
                     /translation="MNKIDEVLELLKQRERESEKPKNVILWVLAIVGAVAAVAGIAYA
                     VYRFVTPDYLEDFEDDFDDDFDDYFEDEDEDK"
     gene            2701..>4144
                     /gene="rlmD"
                     /locus_tag="PLN77_08970"
     CDS             2701..>4144
                     /gene="rlmD"
                     /locus_tag="PLN77_08970"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRM44433.1"
                     /translation="MKKGEIYEGVVERIDFPNKGRVHVEGETVTVKNAIPGQKIRFQI
                     NKKRKNRMEGRLLEVTEKSPMEKRDPVCSIFPSCGGCMYQTMSYEDQLAMKAGQVKKL
                     LDDALVEAGQVNEAGEADYPFLGIKGSPKEFAYRNKMEFSFGDEYKDGPLSLGLHKKG
                     STYDVLTACDCKIVHEDFTKILTCVLAYFKERNASYYHKISHEGYLRHLLVRRAENTG
                     EILVNLVTTSQEEWDLMPLVQEVLGLPLEGKVVGFLHIINDSLADVVKSDETKVLYGQ
                     DYFYEELLGLKFKITPFSFFQTNSLGAEVLYSTAREFVGETKDRTIFDLYSGTGTIAQ
                     ILAPVAKEVIGVEIVEEAVTAAKENAEANGLTNCSFLAGDVLKVIDEIEEKPDFIVLD
                     PPREGIHPKALPKIVDLYRCEKMLYISCKPTSLARDLVEFAKYGYRVEKVCCVDMFPG
                     TVHVETVVLLSKGEVDSKKIRVEFSLEDMDM"
BASE COUNT         1196 a          876 c         1140 g          932 t
ORIGIN      
        1 agctgggaga gcatctgcct tacaagcaga gggtcatagg ttcgagccct ataggcccca
       61 tattttatga aaaacggtgt tgaaaatgaa tatggcgaga tagctcagtt ggctagagca
      121 cgcggttcat acccgcggtg tcgagggttc gaatccccct ctcgctactt gtggaaccct
      181 tgagaaatca agggttcttt tggctttatg gacagtatga tgatatggct gtcatgtcaa
      241 aatcgtgtct tgaaatccac agggatttgt gagaaaataa gactacctaa gagcttgttt
      301 tgggctctga aagtaaataa aacagtaatg gatagaagaa agataaggaa aaaacaatga
      361 tagagtttaa taaaccacct tttattggaa cagaactgga atacatccgc caggcggcag
      421 aggaaaacaa caagctgtgc ggcgacggtc cttttacgaa gaaatgttct gcctggatgc
      481 aggacaattt caacgtaaaa catgcgatgc ttacgacatc ctgcacccat gcactggaaa
      541 tggcagctta cctgtctgag attcagccgg gcgacgaagt gatcatgccg tcctacacgt
      601 ttgtttccac tgcagatgcc ttcgttctgc gcggagcaaa aatcgtctac gtggatatcc
      661 gtccggatac aatgaacatc gacgagacca agattgagga tgccatcacc gaaaaaacaa
      721 aagcgattgt tccggttcac tatgcgggcg ttgcctgtga aatggatacc atcatggata
      781 ttgcaaaacg ccataacctg aaggttgtag aggatgcggc acagggcgtg agcgcgtact
      841 acaagggaaa agcccttgga acgatcggcg attttggctg ctacagcttc catgagacga
      901 aaaactatac gatgggagag ggcggcgcgc tgctgttcca ggatgagaat tatctggaaa
      961 aagcagagat ccttcgtgaa aagggaacga accgcagcaa gtttttccgc ggacagatcg
     1021 acaaatatac ctggatcgac tacggttcct cctatctgcc gagcgatatg aatgcggctt
     1081 acctgcttgc agaactcgag gaacatgaga aaatcgacag aaagcggatg gccatctaca
     1141 actattacca tgagcagctc cgtccgctgg ctgaggctgg aaagatcgag cagccggttg
     1201 tgccggaagg ctgtgtacat aatgcacata tgtacttcat caaggcaaga aatctggaag
     1261 tgcgtacaaa actgatcaag tatatgaaag agcgcggtgt gatgtgtgtc ttccattacg
     1321 ttccgctcca tacctccccg gccggacaga agttcggtgt tttccacgga gaggacaaat
     1381 acacaacaaa agagagcgag cgcctgatgc gtctgccgat gttctacagc ctgtctgaac
     1441 aggatatggc atatgtcgta gagtgcctca tggactttaa agagtggtaa catttcatgt
     1501 atgagaagtt tttaaactac tttacggact atattttcgt gaaggatgag ccgcagaaag
     1561 ccgatgtgat ttttgtgccg ggaaacggat atccgcagat ggcggcgcac gcggcagaac
     1621 tctggaaaga aggatttgcg ccgtatgtgc ttccgtcggg aagatacagt actgtgctcg
     1681 ggcatttcgc cggtgtacag gatcttgcgg agcagtacgg cgggaactat gaaaccgagt
     1741 gggagtttct gcggacggta ctgcagaaaa acggcgtgcc ggagaaagcg attctgcgcg
     1801 aagaccaggc gacgtatacg tatgaaaatg cactgcggtc acgggaagtg acagatgcgg
     1861 cgggcatcga ggtaaaaaaa gcgatcctct gctgccggaa tctccatgcg agacggtgta
     1921 ttctgtatta ccagcttgtc tacccggaga cagagttttt tgtctgtcct tcggatattg
     1981 agttcaccag agaaaactgg catacaaagg gtgaatggat cgaccaggtg ttaggagaaa
     2041 tggagcgctg cggcggtcag ttccacaaga ttctgcggga actgccggcg gattctgaat
     2101 tttaacacca gtagacaaga agtcccccgc ttctaaagcg gtgggatgaa ttgttaaaag
     2161 aaacataacg gcagtggtag gcggagagaa atcggtatcc tccactgaca tgaggaaagt
     2221 cacagtcgaa ctattctcga catgtcagac tccgtgaaaa taccgactgt aagttgaaac
     2281 gaaaagattt ataaaaaaga gcggaaattg tggtaaaatt gcggctcgta tggtatacta
     2341 tcgttaagtt aagtttctaa tctaaagcaa aggttggtgg acagtatgaa taagattgat
     2401 gaagtattag agcttttaaa acagagagaa cgtgagagcg aaaaaccgaa aaacgtgatt
     2461 ttatgggtcc ttgccattgt cggagcggta gcggcagttg caggaatcgc atacgcagta
     2521 taccggtttg taactccgga ttatctggaa gacttcgaag atgatttcga tgacgatttc
     2581 gacgattact tcgaggatga agacgaagat aagtaggatg gaaaaaagaa actccggtgc
     2641 cggaacgtgg taccggagtt tttctgtccg gaaatcagtt tgacatagaa ggagaagaga
     2701 atgaaaaaag gagaaattta cgagggagtc gtggaaagaa tcgatttccc gaataaagga
     2761 cgtgtacatg tggagggcga gacggttacc gtgaaaaatg caattccggg acagaagatc
     2821 cgctttcaga tcaacaaaaa aagaaagaac cgcatggagg gccgcctgct cgaagtaaca
     2881 gaaaaatccc cgatggaaaa gcgcgatccg gtctgcagta ttttcccgtc ctgcggcgga
     2941 tgcatgtacc agacgatgag ttatgaggat cagcttgcaa tgaaggccgg tcaggtgaaa
     3001 aaactgctcg acgatgccct cgtggaggcc ggccaggtca atgaggcggg ggaggccgat
     3061 tatccgttcc ttggaatcaa gggaagtccg aaggagtttg cataccggaa taaaatggag
     3121 ttttccttcg gcgatgaata caaggacggc ccgttgtctc tcggacttca taaaaaggga
     3181 agcacctacg atgtcctgac cgcgtgcgac tgcaaaatcg tgcatgagga tttcacaaag
     3241 attctgacct gcgtgctggc gtactttaaa gagcggaacg caagttacta ccacaagata
     3301 agccacgagg gttacctgcg ccacctgctg gtgcgaagag ccgagaatac cggagaaatc
     3361 ctggtaaatc tcgtcaccac aagccaggaa gagtgggatc tgatgccgct cgtgcaggaa
     3421 gtgcttggtc tgccgctgga aggaaaggtt gtcggattcc tccatatcat aaatgattcc
     3481 ctggcagatg ttgtaaagag cgatgagaca aaagtgctct acggacagga ctacttctat
     3541 gaagaactcc tcggactgaa attcaagatc accccgttct ccttcttcca gaccaattcc
     3601 cttggcgcgg aagttctcta cagcaccgcc cgcgagttcg tcggcgagac gaaagaccgc
     3661 acgatcttcg acctctacag cggaaccgga accatcgccc agatccttgc accggtcgca
     3721 aaagaagtga tcggcgtgga aatcgtggaa gaagctgtta cagctgcaaa agagaacgca
     3781 gaggcaaacg gccttaccaa ctgcagtttc ctcgccggag acgtgctgaa ggtgatcgat
     3841 gaaatcgaag aaaaaccgga cttcatcgtt cttgatccac cgcgcgaagg aatccatccg
     3901 aaggctctgc cgaagatagt tgatttatat aggtgtgaaa aaatgcttta catctcctgc
     3961 aagccgactt ctcttgcgcg ggatctggta gagtttgcaa aatatggata ccgtgtagaa
     4021 aaagtctgct gtgttgatat gtttccggga acggtgcatg tggagacggt tgtcttgctt
     4081 tccaagggag aggtcgactc gaaaaagatt cgggttgagt tctctttgga agatatggat
     4141 atgt
//
LOCUS       DAOMUF010000178         4112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6096_length_4112_cov_3.007148, whole genome shotgun sequence.
ACCESSION   DAOMUF010000178 DAOMUF010000000
VERSION     DAOMUF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6096_length_4112_cov_3.007148"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLN77_08975"
     CDS             <1..550
                     /locus_tag="PLN77_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HRM44434.1"
                     /translation="HVMTRRDCYTYRGNCFAWLLSQGILSSSEEVSDWAVPYLVFCHY
                     APYDFMLRPAETPKKGIPVPLFNLVYHDCVIEPWMMDRVTKDEDYMLYALLAGGAPYL
                     VRDGAYPNTDGAFDGEKISLEEMTERCRVVTELHEKTALLELVRHECMTADGSVQKSE
                     FSDGTYVICDFAEQIYEIGYGE"
     gene            746..2998
                     /gene="pflB"
                     /locus_tag="PLN77_08980"
     CDS             746..2998
                     /gene="pflB"
                     /locus_tag="PLN77_08980"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HRM44435.1"
                     /translation="MREFEQWDGFTGRLWKEGIDVRDFIQNNYTPYDGDEKFLEGPTE
                     ATDRLWGKLQELQKEERAKGGVLDMETDVVSGITAYGAAYIDDSMKDLEQVVGLQTDK
                     PLKRAFMPYGGIKMAEQSCETYGYKPSEKLHEIFTKYHKTHNQGVFDIYTPEMLKARH
                     NKILTGLPDTYGRGRIVGDYRRVALYGIDYLIERKKADFAATNRQGMRRGDFQLREEI
                     ADQVRALQDMKVMAQSYGYDISEPAKNAREAVQWLYFGYLAAIKTQNGAAMSVGRVST
                     FLDIYIERDIEKGILTEKEAQELIDHLVMKFRMVKFARIPSYNQLFSGDPVWATLEVA
                     GMGIDGRSIVTKNDFRFLHTLENMGPSPEPNLTVLYSENLPEAFKKYAAHISVQTSSI
                     QYENDDVMRPVWGDDYSICCCVSATETGKEIQFFGARANLAKCLLYAINGGKDEKTKQ
                     QVAPEYQPITSEYLDYDEVMKKYDVMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMALI
                     DTDVRRTFATGIAGFSHVVDSLSAIKYAKVKVIRDEEGMAVDFETTGDFPRYGNDDDR
                     ADEIAVWLLRTFMDKLKYCHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLS
                     PGANPSYGAEKSGLLASLNSVAKLPYEDALDGISNTQTINPGALGHNDEERTENLVHV
                     LDGYFEQGAHHLNVNVFGTEKLIDAMEHPEKPEYANFTIRVSGYAVKFIDLTKEQQMD
                     VIARTCHDHM"
     gene            3245..>4112
                     /locus_tag="PLN77_08985"
     CDS             3245..>4112
                     /locus_tag="PLN77_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRM44436.1"
                     /translation="MKKKIVAAFCLAMVMTVGGCSGAGTAAQPESSGSQTQPASVSES
                     SSSSDASEENSTQESASSTASSDAADASSNRDIFAMDTYMTVTAYGPNAETAVDQAQQ
                     EIERLDALLSTGEETSEVAQINANGGGTLSEDTTYLLERSLDLYDSTNGVFDIAIYPI
                     MDAWGFTTGNYTIPSDDTIESLLPLTDANDILYDKDSASISFAKDGMKIDFGGIAKGY
                     TSGRIADIYRECGVTSGLINLGGNVQVVGTKTDGSKWRVAVQSPEAEDDYLGILSTAD
                     RAVITSGGYERYF"
BASE COUNT         1164 a          926 c         1068 g          954 t
ORIGIN      
        1 gcatgtgatg acgagaagag actgctatac gtaccgcgga aactgcttcg cctggctgct
       61 ctcacagggg attctctcaa gttccgagga ggtcagcgac tgggcggttc cgtatctggt
      121 attctgccat tatgcgccgt acgactttat gctgcgcccg gcagagacac cgaaaaaagg
      181 aatcccggtg ccgctcttta atcttgtgta tcatgactgc gtaatcgagc cgtggatgat
      241 ggaccgtgtg acaaaagacg aggactatat gctgtatgcg cttcttgcag gcggtgcacc
      301 gtatcttgta cgggacggcg catatccgaa tacagatggt gcatttgacg gggagaaaat
      361 cagtctggaa gagatgacag aaagatgccg cgttgtaacg gagctgcatg aaaaaacggc
      421 actcctggaa ctggtacgtc acgagtgtat gacggcggac ggaagcgtac agaagagtga
      481 attttccgat ggaacatatg ttatttgtga ctttgcagag cagatatatg aaatcggata
      541 cggggaataa aagaaaagcg ccacatgtag attttgttct tataaaaata caagattttg
      601 tggttttcct tgtatttttt caagtgcata tcttgcgcgg aaagcagaga tttgttatat
      661 tatgcatgcg gaggtgttca tgcccggtgc aaagaagatg ccggagcgtg acgaaacaaa
      721 ataacaccag aaaggaacaa atcaaatgag agaatttgaa cagtgggatg gctttaccgg
      781 aagactctgg aaagaaggca tcgacgtacg tgactttatt cagaacaact atactccata
      841 tgacggagat gagaaattcc tggagggacc aacagaggct acagaccgtc tgtggggcaa
      901 attacaggaa ctccagaaag aagagcgtgc aaaaggcggc gttctggata tggagacaga
      961 cgttgtaagc ggaatcacag cttacggcgc agcatatatc gatgacagca tgaaagatct
     1021 ggagcaggta gttggtcttc agacagacaa accgctgaaa cgtgcattta tgccgtatgg
     1081 tggaattaaa atggctgagc agtcctgcga gacttacggc tacaaaccaa gcgagaagct
     1141 tcatgaaatt ttcactaaat atcacaaaac acataaccag ggtgtattcg acatctacac
     1201 accagagatg ctgaaagccc gtcataataa aatcctgacc ggtctgccgg atacctatgg
     1261 ccgtggccgt atcgtaggcg actaccgtcg tgttgctctg tacggtattg attacctgat
     1321 cgagcgcaaa aaagcagact tcgctgcaac aaatcgtcag ggaatgcgtc gtggtgactt
     1381 ccagttaaga gaagagatcg ctgaccaggt cagagcactg caggatatga aagttatggc
     1441 acagtcctat ggctatgaca tttccgagcc ggctaagaac gcaagagaag ctgttcagtg
     1501 gctgtacttc ggataccttg cagctatcaa aactcagaac ggtgctgcaa tgagcgttgg
     1561 ccgtgtatct actttccttg acatctacat cgaaagagat attgaaaaag gaatcctgac
     1621 tgagaaagaa gctcaggagc tgatcgacca tctggtaatg aaattccgta tggttaaatt
     1681 cgcaagaatt ccgtcttaca atcagctgtt ctccggtgac ccggtatggg ctactctgga
     1741 agttgccggt atgggaatcg acggacgttc tatcgttaca aagaacgatt tccgtttcct
     1801 gcatacactg gaaaacatgg gaccgtcccc agagccgaac cttactgttc tgtactctga
     1861 gaatcttccg gaagctttca agaaatatgc agctcatatt tctgtacaga caagctccat
     1921 ccagtatgag aacgatgatg taatgcgtcc ggtatggggc gatgattact ccatctgctg
     1981 ctgtgtatct gctaccgaga ccggtaagga gatccagttc ttcggcgctc gtgcaaacct
     2041 tgcaaaatgc cttctgtacg ctatcaacgg cggtaaagac gagaagacaa aacagcaggt
     2101 tgctccggaa tatcagccga tcaccagcga gtatctggac tatgatgagg ttatgaagaa
     2161 atacgacgtt atgatggact ggctggctca tctgtacgtt ggaactctga acatgatcca
     2221 ctacatgcat gacaaatact actatgaagc agcagagatg gctctgatcg acaccgatgt
     2281 aagacgtacc ttcgcaaccg gtatcgcagg attctctcac gtagtagact ccttaagcgc
     2341 aatcaaatac gctaaagtta aagttatccg tgacgaagaa ggaatggctg ttgacttcga
     2401 gacaaccggc gacttcccaa gatacggcaa cgacgatgac agagcagacg agatcgctgt
     2461 atggctgctt cgtaccttca tggacaaact gaaatactgc catacctatc gtgattccga
     2521 gccgacaacc tctatcctta ccattacttc caacgttgta tatggtaaag caactggttc
     2581 tctgccggac ggaagaaaag ccggtgagcc tctgtctcca ggagctaacc cgtcttacgg
     2641 cgcagagaag agcggacttc ttgcttccct gaactctgta gcaaaactgc cgtacgagga
     2701 tgctctggac ggaatctcca acacccagac catcaacccg ggcgcacttg gccacaacga
     2761 cgaagagcgt accgagaacc tggtacacgt tctggacgga tacttcgagc agggtgcaca
     2821 tcacctgaac gtaaacgtat tcggaaccga aaaactgatc gacgcaatgg agcacccgga
     2881 gaaaccggag tatgcaaact tcacgatccg tgtatccggt tatgcagtta aattcatcga
     2941 cctgacaaaa gagcagcaga tggacgtaat tgcacgtacc tgccacgatc atatgtaatt
     3001 tctgaaaatg ttatttctaa agcccccgcg gccgcagcaa tgcggctgtg ggggcttttt
     3061 gtatatcggt gcttgaattc cagtaaaatc cacttgattt agagtacata aaatgagaaa
     3121 atttatcaga tgcagaacgc taattgaaaa acttttatca agtaattcat agcgtaagtt
     3181 acaagttaat gttataatac caaagttaga atttcctaac acaagaaaaa atggagtttt
     3241 atttatgaaa aagaaaattg ttgcagcatt ttgtcttgcc atggtgatga cggtcggcgg
     3301 atgttccggt gcggggacag cagcgcagcc ggagagcagc ggaagtcaga cacagccggc
     3361 gtctgtttcg gagtcttcga gcagttctga cgcttcggaa gagaattcca cgcaggaatc
     3421 cgcatcatcc acagcttctt ccgatgccgc agatgcatcg agcaaccgtg atatttttgc
     3481 gatggatacg tacatgacgg tcactgccta tggtccgaat gcagaaacgg cggtggatca
     3541 ggcgcagcag gagattgagc ggctggatgc acttctgtcg accggagagg agacgagtga
     3601 agtagcgcag atcaatgcaa acggcggcgg aaccttatcg gaggatacca cctatctgct
     3661 ggaacgctcg ctggatctgt atgacagcac aaacggcgtt ttcgacatcg caatttatcc
     3721 gatcatggac gcctggggtt ttacaaccgg aaattatacg attccttcgg atgacacgat
     3781 cgaaagcctc cttccgctga ccgatgccaa tgatatcctt tacgacaagg acagcgcatc
     3841 catcagcttt gccaaggacg gcatgaaaat tgacttcggc ggaatcgcaa agggttatac
     3901 atccggcaga attgcggata tttaccggga atgcggtgtg acgagcgggc tcatcaacct
     3961 cggtggaaac gttcaggttg tcggcacgaa aacggatggc agcaaatggc gtgttgccgt
     4021 gcagagtccg gaagcggagg atgattatct cggcattctc tcgacggccg accgggcggt
     4081 tattacttct ggcggctatg agcggtattt cg
//
LOCUS       DAOMUF010000179         4085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6161_length_4085_cov_4.581141, whole genome shotgun sequence.
ACCESSION   DAOMUF010000179 DAOMUF010000000
VERSION     DAOMUF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6161_length_4085_cov_4.581141"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLN77_08990"
     CDS             <1..291
                     /locus_tag="PLN77_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HRM44437.1"
                     /translation="TIAYLSSMKYYFKPIRELPTGRGFADFVYLPKLEFRSDYPALLV
                     ELKWNHSAHTALNQIKEKKYIASLEPYIGDILLVGINYDKKTKKHECLIEKA"
     gene            complement(387..716)
                     /locus_tag="PLN77_08995"
     CDS             complement(387..716)
                     /locus_tag="PLN77_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM44438.1"
                     /translation="MSIMKNINKAKVMVLKDQVSYQEGQIVSKTLVQNESVSITLFAF
                     DKGEEISTHESGGDAFVTCLDGTGEITIDGVKYELHEGESIVMPAKHPHAVYGKEQYK
                     MLLVVVF"
     gene            complement(932..2566)
                     /gene="hcp"
                     /locus_tag="PLN77_09000"
                     /gene_synonym="priS"
     CDS             complement(932..2566)
                     /gene="hcp"
                     /locus_tag="PLN77_09000"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRM44439.1"
                     /translation="MEQNMFCYQCQETAGCSGCTKSGVCGKRPETAALQDLLIWTTKG
                     LSAVTTQLRREGKTVDASVNHLITENLFTTITNVNFDDDTIRARIEATLETKALLAQL
                     ADCSALPEAALWNGTTDEFAAKAVTVGVLSTENEDIRSLRELITYGLKGLAAYTSHAN
                     VLLKENEEIDAFLQRALAATLDDSLSAEELTALALETGSYGVQGMALLDEANTSAYGN
                     PEITTVDLSVGTRPGILVSGHDLRDLEMLLEQTVNTGIDVYTHSEMLPAHYYPAFKKY
                     EHFKGNYGNAWWKQKEEFEAFNGPILMTTNCIVPPKDSYKNRIYTTGAVRYPGCPHID
                     GVIGETKDFSLLIEQAKHCAPPTELEQGTIVGGFAHAQVLALADQIVDAVKSGAIKKF
                     VVMAGCDGRAKSRSYYSDFAQALPKDTVILTAGCAKYKYNKLNLGDINGIPRVLDAGQ
                     CNDSYSLAVIALKLKEVFGLDDINDLPIIYNISWYEQKAVIVLLALLSLGVKNIHLGP
                     TLPAFLSPNVAALLVENFGIAGIDTVENDMELFFGK"
     assembly_gap    2702..2801
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2824..3507)
                     /locus_tag="PLN77_09005"
     CDS             complement(2824..3507)
                     /locus_tag="PLN77_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRM44440.1"
                     /translation="MKSLADAVFPAEVFSLPLFHGISETGRAALVSSGWLRTCSFEKN
                     QTIYHMGDRVSELGIVLSGSVFVENTDLWGNRSILSKITPGQVFAETYAFCREPMMVN
                     VTAAEASTVLFFNLRNLDQTADGDDAWLSQLRSNMLRISMQKNLTLSNRIFCTTPKTI
                     RERVLVYLAAQSAKTGADTFTIPFDRQGLADYLNLDRSALSKELGKMQKEGILEFHKN
                     KFTLRHPSL"
     gene            complement(3491..3670)
                     /locus_tag="PLN77_09010"
     CDS             complement(3491..3670)
                     /locus_tag="PLN77_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate--pyruvate lyase"
                     /protein_id="HRM44441.1"
                     /translation="MLSCTYEVVAIDGDYAHLRRNDIEPEDNETKLVARALLPPEIME
                     GSILKYEMLQYEIIG"
BASE COUNT         1007 a          906 c         1058 g         1014 t
ORIGIN      
        1 acgattgctt atttaagctc catgaagtat tacttcaagc cgattcgtga actgccgaca
       61 ggaaggggct ttgcagactt tgtttatctt ccgaaactgg aatttcgcag tgattacccg
      121 gcacttctcg tagaactgaa atggaatcat tctgcccata cggcactgaa tcagatcaaa
      181 gagaaaaagt acattgcatc attggaacct tatattggag acattctgct ggttggaatt
      241 aattatgata aaaagacgaa aaagcatgaa tgtctgatag aaaaagctta aatgaataaa
      301 aaggcaaggc agcatatcac aggaaatcct gaggcatgct gccttatttt gccaggcata
      361 tcaataattg gttcaaaaag gatacgttag aataccacta ccagaagcat cttatactgc
      421 tcttttccat acactgcatg cgggtgtttt gccggcatga cgatggattc gccttcgtgg
      481 agttcgtatt ttacgccgtc gatggtgatt tcaccggttc cgtcgaggca ggtgacgaag
      541 gcgtcgccgc cggactcgtg ggtgctgatt tcttcgcctt tgtcgaaggc aaataaggtg
      601 atgctgacgg attcattctg taccagtgtt ttgctgacga tctggccttc ctggtaggaa
      661 acctgatctt ttaagaccat aaccttggct ttattgatat ttttcatgat gctcatagtt
      721 cataaatctc cgtgatagaa tgagaacgtg tctgaaaaaa ttcccaggca cgttctaaaa
      781 atgtgtgatt aagatgagat gactcatcgg tgtcgggcgt gtatagaaat ggaatctgag
      841 gtccgttttc tgatatgccc aaattcaaag atatttttgt ctgaacatat acagtatacg
      901 tggaaaaatg aaagaaagca aggggttttc tttatttccc gaaaaacaat tccatatcgt
      961 tttcaacggt atcgattccg gcgatgccga agttttcaac gagaagagct gcaacattcg
     1021 ggcttaaaaa tgccggaagt gtcgggccga ggtggatgtt cttgacaccg agagacagca
     1081 gtgccagaag aacaatcaca gctttctgct cgtaccagga aatattgtag atgatcggca
     1141 gatcgttgat atcgtcgagg ccgaagacct cctttaattt cagtgcaatc acggcaagag
     1201 agtaggaatc attgcactgg ccggcatcga ggacgcgcgg aatgccgttg atgtcgccga
     1261 ggttcagctt gttgtatttg tattttgcac agccggcggt cagaatcacg gtgtctttcg
     1321 gcagtgcctg tgcaaaatca gagtaatagc tgcggctctt tgcacggccg tcacatccag
     1381 ccatgacaac gaattttttg atcgcgccgg atttcacggc atcgacgatc tggtctgcca
     1441 gggcgagtac ctgtgcatgg gcgaagcctc cgacaatggt tccctgctcg agttccgtcg
     1501 gcggtgcgca gtgctttgcc tgttcgatga gcagagagaa atcttttgtc tcaccgatga
     1561 cgccgtcgat gtgcgggcat cccggataac gaaccgctcc ggttgtgtaa atacggtttt
     1621 tgtagctgtc cttcggcggc acgatacagt tcgtcgtcat cagaatcggg ccgttgaagg
     1681 cttcaaattc ttctttctgc ttccaccagg cgttaccata atttccctta aaatgttcat
     1741 attttttgaa cgccggatag tagtgtgcag gcagcatttc ggagtgggtg taaacatcga
     1801 tgccggtgtt cacggtctgc tcgagcagca tctcgaggtc gcgcagatca tgaccggaga
     1861 cgagaatacc cgggcgggtg ccgacactga gatcgacggt ggtgatctcc ggattgccgt
     1921 aagcggaagt attggcctca tcgagcagtg ccattccctg cacgccgtag gagccggtct
     1981 cgagtgcgag agccgtcagt tcctctgcgg agagggaatc gtcgagcgtt gcggcgagag
     2041 cacgctgcag gaaggcatcg atttcctcgt tttcttttaa gagaacattg gcatggctgg
     2101 tgtacgcggc aagtcctttc agaccgtagg tgatgagctc gcgtaaagag cggatgtcct
     2161 cattttccgt ggaaagtacg ccgacggtaa ccgcttttgc ggcaaactca tcggttgtgc
     2221 cgttccagag ggcggcctcc ggcagagcgg agcagtcggc aagctgagcg agaagcgctt
     2281 tggtctcaag cgttgcctca attcgtgcgc ggatcgtatc atcatcaaag ttcacgtttg
     2341 tgatggtcgt aaacaggttt tcggtgatga gatgattgac gctggcatcg acggtttttc
     2401 cttcgcggcg gagctgtgtc gtcacggcgg aaagtccttt cgtcgtccag atcagcagat
     2461 cctgcagcgc cgctgtctcc gggcgctttc cacagacacc ggattttgta cagccggagc
     2521 agccggcagt ttcctgacac tggtagcaaa acatattctg ttccataaaa atagttcctc
     2581 ctgtagggta tttccgaggg gttaagcgca gagggaaaaa cagatctgcg cggagtttca
     2641 taatttacac gtacagaata tcggatataa caggaaaagt atgtggttat aaccacggaa
     2701 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaatttac gctgcggtac
     2821 cgcctagaga gacggatgac gcagcgtaaa tttgttttta tgaaattcga ggattccctc
     2881 tttctgcatt tttccgagtt ctttggagag ggcgctccgg tcgaggttca ggtagtcggc
     2941 gaggccctgg cggtcgaagg ggatcgtaaa ggtgtcggcg ccggttttgg cagactgcgc
     3001 ggcgaggtag accagcacgc gctcgcggat cgtctttggt gtagtgcaga aaatgcggtt
     3061 ggagagggta agatttttct gcatggagat gcggagcatg ttactgcgaa gctgggacag
     3121 ccatgcatca tcaccatcgg cggtctggtc gaggtttcga agattgaaaa agagaaccgt
     3181 cgaggcttcg gctgccgtga cattcaccat catcggctcc cgacagaagg catacgtttc
     3241 ggcaaatacc tggccggggg tgatcttgct taagatactg cggttgcccc agagatccgt
     3301 attctccacg aaaacgctgc cggacagcac aatccccagt tcggacacgc ggtcacccat
     3361 gtgataaatg gtctggtttt tctcaaacga acacgtccgc agccacccgc tcgaaacaag
     3421 agccgcccgc ccggtttccg agatgccatg aaagagcggg agagaaaaca cctcggccgg
     3481 aaaaacagca tcagccaatg atttcatact gcagcatctc gtatttcaaa atactgccct
     3541 ccataatttc cggcggcagc agcgcacggg caaccagttt cgtttcattg tcctccggtt
     3601 cgatgtcatt gcggcgtaaa tgcgcgtaat caccatcgat ggcgactact tcgtaggtgc
     3661 aggataacat aagaaaacaa cctccttttt ctttacatga tacctattat accgtgtgca
     3721 gaaacggatg tcgatggaat ttttaatgtc ggaaacaaaa gtctatgcta tacttacagc
     3781 agggtatgac agaaaagaaa ggatggaagg taggatcctg ggggaagaac ttgaactgga
     3841 agtctgctcg gtgctgagaa aaatccggga agatatgtag ctcattatca tcagcggcta
     3901 tgcggaattt tcttatataa taggaaagtt cgacatctgc tcagagaaat ccctaaacat
     3961 gtggaggata aaaaaactga ttttctaaca gagcttctgc catggtccaa gtctttacca
     4021 gaacatatcg ggaagcccag acagcaataa gatttcacca gctaacagag gatttttctt
     4081 tacac
//
LOCUS       DAOMUF010000180         3974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6388_length_3974_cov_2.762439, whole genome shotgun sequence.
ACCESSION   DAOMUF010000180 DAOMUF010000000
VERSION     DAOMUF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6388_length_3974_cov_2.762439"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PLN77_09015"
     CDS             complement(<1..1142)
                     /locus_tag="PLN77_09015"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRM44442.1"
                     /translation="MRKYNRIFLIVLDSLGIGAMADSEKFGDVGVNTFGHIWESQGGL
                     EIPNLRRLGMGNLCGVTEDEAKGYARRLNEASNGKDTMTGHWEMMGIYTTKPFVTFTD
                     HGFPKELIDELEKRTGRKIIGNKAASGTEILDELAEREIENGELIVYTSADSVLQICG
                     NEETMGLETLYHYCEIARELTMKDEWKVGRVIARPYVGKKKGEFVRTANRHDYAVKPT
                     GKTVLNAMQEAGYDVISIGKINDIFCGEGIMESLKSKSSVHGMEQTIETAGKDFTGLC
                     FVNLVDFDALWGHRRNPEGYGKEIEKFDVNLGKLLEQLKDDDLLLITADHGNDPTYTG
                     TDHTREQVPLLAYSPSMKESGLQETKDTFAVIGASVAENFGVKMPEG"
     gene            complement(1162..3006)
                     /locus_tag="PLN77_09020"
     CDS             complement(1162..3006)
                     /locus_tag="PLN77_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44443.1"
                     /translation="MAARAYSGKKPKNLKHTLRVFLSYLGRHKKMLAVVAVLVTISAG
                     ANLLGTYMIRPVVNGLADGDVHTLLRGVLITALIFGCGALAAYGYTQTMVKAAQQVVF
                     DIRRDLFEHVQTLPLQFFDSRRHGDIMSLFTNDIDTMADALNNSFAMVIQSFIQIVGT
                     LTLLYILNWRLSLIVTVCYGIMFWYIKFSGKRSKGYYTKQQNSLGELNGYIEELITGQ
                     KVVKVFHHEEESFTEFCKKNEELRKAGTGAQGYAATMVPVVVSISYVNYAIVAVLGGL
                     LALHGKADIGSLASYLVFVRQAALPINQFTQQSNFLLSALAGAERVFDVMSLEPEIDE
                     GKVELVNVKEENGALAVCEETTGRWAWKRPDGTMTELKGDVRFENVDFGYTADRLILK
                     NISLYAKPGQKIAFVGSTGAGKTTITNLINRFYDVQGGAVVYDGIDVKDIEKDALRHS
                     LGIVLQDTHLFTGTVAENIRFGKLDATQEEIERAAKIANADSFIRRLPNGYDTMLTSD
                     GANLSQGQRQLLAIARAAVADPPVLILDEATSSVDTRTEALIEKGMDQLMEGRTVFVI
                     AHRLSTVRNANAIMVLEQGNIVERGDHDALLAQKGKYYQLYHGMFELS"
     gene            complement(2987..>3974)
                     /locus_tag="PLN77_09025"
     CDS             complement(2987..>3974)
                     /locus_tag="PLN77_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44444.1"
                     /translation="VLILWFGGNMILKKQLAVGNLTGFLSYVMQVMNSMMMIANVFLL
                     LTRSMASAHRIVEVLDEKIVLTSPENGVTEVTEGSVEFDNVSFKYKEEAKEYALSDVT
                     LKIKAGQTVGILGGTGSSKTTLVQLIPRLYDTSRGTVRVGGKDVRAYDLAALRDAVNI
                     ILQKNVLFSGTVRENLKWGNVDASDEEIWEACRAACADEFLNRMPDGLDTVLEQGASN
                     LSGGQKQRLCIARALIGKAKILIFDDSTSAVDTATEKKIREALAARKNVTKIIIAQRV
                     TSVMNTDQIIILDDGKVHRIGTHAELLADDPIYQEIYASQMKGGDENGSESL"
BASE COUNT          775 a         1142 c          990 g         1067 t
ORIGIN      
        1 ccctccggca tttttacgcc gaagttctct gcaacggatg cgccgatcac agcgaaggtg
       61 tccttcgtct cctgaaggcc gctctccttc atagacggtg agtacgcaag gagcggcacc
      121 tgttctctgg tgtggtccgt tccggtataa gtcgggtcgt tgccgtggtc tgccgtgatc
      181 aggagcagat cgtcgtcttt taactgctcc agcagctttc caaggttcac atcgaatttt
      241 tcaatctctt ttccgtagcc ttccgggttt cttctgtggc cccagagtgc atcgaaatcg
      301 acgaggttga caaagcacag tccggtaaaa tcttttcctg ccgtctcaat cgtctgctcc
      361 atgccgtgga cgctgctctt ggatttcaga ctctccatga tgccctcgcc gcagaaaata
      421 tcgttgattt ttccgatgct gatgacatca tatcctgcct cctgcatcgc gttgagcacg
      481 gtttttcccg tcggtttgac agcgtagtcg tgacggtttg cggtgcggac gaactctcct
      541 tttttctttc cgacgtacgg tcttgcaatg acacggccga ctttccactc gtctttcatg
      601 gtcagctcac gggcaatctc gcagtaatga tacagcgtct ccaatcccat cgtctcctcg
      661 ttgccgcaga tctgcagtac ggaatccgcc gaggtataga caatcagttc tccgttttcg
      721 atctcgcgct ctgccagctc atcgaggatc tccgttccgc tcgccgcctt attgccgatg
      781 atctttctgc cggttcgttt ttccagctcg tcgatcagtt ctttcggaaa tccgtggtcg
      841 gtgaacgtaa cgaacggctt tgtcgtgtaa attcccatca tctcccagtg gccggtcatg
      901 gtgtcttttc cgttgctcgc ctcgttcagt cttctggcgt aaccctttgc ctcatcctct
      961 gtcacaccgc agagatttcc catgccgagg cggcgcaggt tcgggatttc aaggcctccc
     1021 tgactctccc agatgtggcc gaaggtattg acaccgacat ccccaaactt ctccgaatcc
     1081 gccatcgccc cgattccgag agaatccagg acgatcagaa aaatacggtt atattttctc
     1141 atactcttta ctcctttctt tttaactcag ctcaaacatt ccatgataga gctgataata
     1201 ttttcctttc tgtgccagaa gtgcgtcgtg gtcgccgcgc tctacgatat ttccctgctc
     1261 cagcaccatg atcgcattgg cgtttcgcac ggtgcttaag cggtgggcga tgacaaatac
     1321 ggttctgccc tccatgagct ggtccattcc tttttcgatc agcgcctcgg ttctggtgtc
     1381 aacggaggat gttgcctcgt caagaatcag caccggcgga tcggcaacgg cggcacgggc
     1441 aatcgcgaga agctggcgct ggccctggga caggttggct ccgtcggagg tcagcatcgt
     1501 gtcgtagccg ttcggcagac ggcggatgaa ggaatccgca ttcgcaattt tggccgcacg
     1561 ttcaatttct tcctgcgtgg catcgagttt tccaaaacgg atgttttctg ctacggttcc
     1621 cgtgaacaga tgggtatcct gcagaacgat accgagggag tggcgcagag cgtctttttc
     1681 gatatctttt acgtcaatgc cgtcgtagac aaccgcgccg ccctggacgt cgtagaaacg
     1741 gttgatcagg ttcgtgatcg tcgtttttcc ggctcctgtg gaaccgacaa aagcaatttt
     1801 ctggcctggt ttggcataca ggctgatgtt ctttaagatc agacggtctg ccgtgtagcc
     1861 gaaatccacg ttttcaaaac gcacatcgcc ctttaattcc gtcatcgttc cgtccgggcg
     1921 cttccatgcc cagcggcctg ttgtctcttc gcagacggca agtgcgccgt tctcttcttt
     1981 tacgttgacc agttccactt ttccctcatc gatttccggc tccaggctca tcacgtcgaa
     2041 gacacgctcc gcaccggcga gcgctgagag aaggaagttg ctctgctggg tgaactggtt
     2101 gatcggaagc gccgcctggc ggacaaagac aaggtagctt gccaggcttc cgatatcggc
     2161 ttttccgtgc agggcgagaa ggccgccgag cactgccaca atcgcataat tgacatacga
     2221 aatgctgaca acgacaggaa ccatcgtcgc cgcatacccc tgcgcgccgg ttcccgcttt
     2281 ccggagctcc tcattctttt tgcagaattc cgtaaagctc tcttcttcgt ggtggaacac
     2341 cttgacgact ttctgtccgg tgatcagctc ctcaatatag ccgttcagtt ctccgaggct
     2401 gttctgctgc ttcgtataat aacccttgct gcgctttccg ctgaacttga tataccagaa
     2461 catgataccg tagcacaccg ttacaatcag cgagagccgc cagttcagga tatacagcaa
     2521 ggtcagtgtt cccacaatct ggatgaagct ctggataacc atcgcaaaac tgttgttcag
     2581 ggcatctgcc atcgtgtcga tatcgttcgt gaaaagactc atgatatcgc cgtggcgtct
     2641 gctgtcgaag aactgcagcg gcagtgtctg gacgtgttcg aacagatcgc ggcggatatc
     2701 aaatacaacc tgctgtgctg cttttaccat ggtctgcgtg tagccgtatg ccgcgagtgc
     2761 gccgcacccg aaaatgagtg ccgtaatcag cacgccgcgc agcagtgtgt gcacatctcc
     2821 gtccgccagt ccgttgacaa ccgggcggat catatacgtt ccaaggaggt tggctcccgc
     2881 gctgattgtt accagtaccg ctacgactgc gagcattttt ttgtgccgtc ccagataaga
     2941 caggaagacg cgcagcgtgt gtttcaggtt ctttggtttc tttccgctat aagctctcgc
     3001 tgccattttc gtctcctcct ttcatctgtg aggcgtaaat ttcctgataa atcgggtcat
     3061 ccgccagcag ttcagcgtgc gtgccgatcc ggtgtacttt tccgtcatca agaatgatga
     3121 tctgatccgt attcatgacg gatgttactc tctgcgcaat gataattttt gtgacattct
     3181 tccgcgccgc cagtgcctcg cggatctttt tctccgttgc cgtgtcgacc gcacttgtcg
     3241 aatcatcgaa gatcaggatt tttgcctttc cgatcagcgc acgcgcgatg cagagacgct
     3301 gcttctggcc tccggagagg ttgcttgcgc cctgctcgag cacggtgtcg aggccgtccg
     3361 gcatgcggtt caaaaattca tccgcacacg ccgcgcggca tgcttcccag atctcctcat
     3421 cggatgcatc cacattgccc catttcaggt tttcacgcac ggttccggag aacagcacgt
     3481 ttttctgcag aatgatattg actgcatcgc ggagagcggc aagatcgtac gcgcgcacat
     3541 cctttccgcc gacgcggacg gtgccgcggc tggtgtcata cagacgcgga atgagctgca
     3601 ccagcgtcgt tttcgaagaa cctgttcctc cgaggattcc taccgtctga ccagctttga
     3661 tcttcagcgt aacatcggag agtgcgtact cttttgcttc ttctttgtat ttaaacgata
     3721 cgttgtcaaa ctccacgctg ccctctgtca cttctgttac accattttcc ggcgaggtga
     3781 gcacgatttt ctcatcgagc acctcgacaa tccggtgtgc gctcgccatg gaacgggtca
     3841 ggagcaggaa gacgtttgca atcatcatca tcgagttcat gacctgcatg acgtagctta
     3901 gaaatccggt caggtttcca acggcaagct gttttttcag aatcatattt ccgccgaacc
     3961 acagaatcag cacg
//
LOCUS       DAOMUF010000181         3960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6416_length_3960_cov_3.084251, whole genome shotgun sequence.
ACCESSION   DAOMUF010000181 DAOMUF010000000
VERSION     DAOMUF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6416_length_3960_cov_3.084251"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..859
                     /locus_tag="PLN77_09030"
     CDS             161..859
                     /locus_tag="PLN77_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44445.1"
                     /translation="MSELVTCKDLTKRFGNHTALDHLNLSLDSGKIIGLLGPNGSGKT
                     TLLKLLNGLLTPSEGEVLIAGNHPGIETKRIVSYLPDKMYLGSWMRVSDLLTYYSDFF
                     EDFDMSRANAMLESLKISPKDRLKTMSKGTKEKVQLILTMSRRAKLYCLDEPIAGVDP
                     AARDYILSTILNNYEPDASILISTHLISDVENILDEVIFIQEGQLKLHASVEDIRMQE
                     GESIDSYFREVFKC"
     gene            853..1686
                     /locus_tag="PLN77_09035"
     CDS             853..1686
                     /locus_tag="PLN77_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44446.1"
                     /translation="MLKKCLKHEWKACWKNMALLNGAAIFMGALGGFGFLFSQLSELP
                     DLLIVLYLFAYVLIVIAASVMTQVLMIQRYYKNLFTDEGYLTNVLPVSATDHILSKML
                     VFGAWSILNALSVLASFAFLLLPSAIRYFTANTLLFYNKTMTFSDFLSYLWAQITCVA
                     DAAFSLIGITNHIGMALFSIVGFFASLFFGILLFYAAISIGSLVSSHKILTAVLSYIG
                     MTTVIQIFGGISIVRVFQDSTPTAVVTTSSVSTLIITVVLCVVLFLICRYILSRRLNL
                     S"
     gene            complement(1760..>3960)
                     /locus_tag="PLN77_09040"
     CDS             complement(1760..>3960)
                     /locus_tag="PLN77_09040"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRM44447.1"
                     /translation="SEIFEALMVITKQEMAGRKRNTGSKKLYYISAEFLIGKLLSNNL
                     INLGMYEEVEKALAAHGRELTEIEEMEMEPSLGNGGLGRLAACFLDSIATLGLPGDGI
                     GLNYHFGLFRQLLVDHKQKEVKNPWITNESWLVRQPVSFAVPYKNFTMHSTLYDIDVP
                     GYNNGCNRLHLFDVDTVDESIVPSDSINFDKHQIQKNLTLFLYPDDSDRAGQLLRIYQ
                     QYFMVSNGAQFILMECEQKGYDLHKLYDHVVIQINDTHPSMVIPELIRLLQERGFTMQ
                     EAIDVVSKTCAYTNHTILAEALEKWPIDYLEEVVPHLMPIIHELDAQAKKKYKDEKVA
                     IIDKDQRVHMAHMDIHYGFSVNGVAALHTEILKNSELKPFYDIYPEKFNNKTNGITFR
                     RWLLHCNRELAQYIESLIGPGFKKDADELEKLLKYKDNKKVLAKLEEIKHNRKLDLKQ
                     FLYETQNIALDENSIIDVQIKRLHEYKRQQMNALYAIYKYMDIKSGNKPKTPITMVFG
                     AKAAPAYIIAKDIIHLILCLQELIDNDPEVSPYFKVIMLENYNVSKAAKVIPAADISE
                     QISLASKEASGTGNMKFMLNGALTLGTEDGANVEIGDLVGKENIYIFGKKPQDVIDLY
                     ADAAYCSKDIYDEDPEIAKLVDFIVSDELLAIGDKVCLERLYKEILNKDWFMTLLDLK
                     EYIKTKERVYKDYENKDAWNKKCLINIAKAGFFSSDRTIAQYNEDIWHLA"
BASE COUNT          886 a          989 c          938 g         1147 t
ORIGIN      
        1 ggacgcttta ttaccgaaga taaggagttg attcatcaga tgaaaaaaga acttgccgcc
       61 gcagaagtat ctgcttttgt tgcccacatg aaacagctcg gcataacccc ggaggaaatc
      121 cggcagctac ttgccgaaac gattgaggag gaagaaaatc atgagtgaac ttgtaacgtg
      181 taaagacctg acgaagcgct tcggaaacca caccgcgctc gatcatctca acctttccct
      241 ggattccggg aaaattatcg gactgctggg gcccaacgga agcggaaaga cgacgctctt
      301 aaagctgctg aacggactgc tgaccccgag tgagggtgaa gtcctcatcg ccggaaatca
      361 tcccggtatt gagacaaaac ggatcgtctc ctatctgccg gataaaatgt atctcggcag
      421 ctggatgcgc gtctcggatc ttttaacgta ttacagcgat ttctttgagg actttgacat
      481 gtcccgcgcc aatgccatgc tggaatcctt aaaaatcagc ccgaaggacc gtttgaaaac
      541 gatgtcgaag ggaacgaaag agaaagtcca gctcatcctc acgatgagcc gccgcgcaaa
      601 gctctactgc ctcgacgagc cgattgcagg cgtcgatccg gcagcacgcg attacatttt
      661 atctacgatt ttaaacaact acgaaccaga cgcatccatc ctcatctcaa cgcatctgat
      721 ttccgatgtc gaaaatattc tcgatgaggt catctttatc caggagggcc agttaaaact
      781 gcacgcctcg gttgaggata tccggatgca ggagggtgaa tcgattgatt cttatttcag
      841 ggaggtattc aaatgttaaa aaaatgtctg aaacatgagt ggaaagcatg ctggaaaaac
      901 atggcgctgc tgaacggtgc cgccattttc atgggcgctc tcggaggctt cggattcctc
      961 ttttcccagc tttctgagct gccggatctg ttgatcgtcc tctatctgtt tgcgtacgtt
     1021 ttgattgtga tcgccgcatc cgtcatgaca caggtgctga tgatccagcg ctactataaa
     1081 aatcttttta ccgacgaagg gtatctgacc aacgtccttc cggtatcggc aacggatcat
     1141 attctctcca aaatgctggt tttcggcgca tggagcatct taaacgcact ttccgttctc
     1201 gcttccttcg cttttttgct tctgccgtcc gcaatccggt attttaccgc aaacaccctg
     1261 cttttttaca acaagaccat gaccttttcc gattttctgt cctatctctg ggcacagatt
     1321 acctgtgttg ccgatgctgc tttttccctg atcggcatca cgaaccacat cggtatggca
     1381 ctgttttcca tcgttggctt cttcgcttcg ctgttctttg gcattcttct gttctatgcc
     1441 gccatcagca tcggaagcct ggtatccagc cacaaaattc tgacggctgt cctctcttac
     1501 atcggcatga ctaccgtgat ccagatcttc ggcggaatca gcatcgtccg tgttttccag
     1561 gactccacac cgactgctgt ggttaccaca tcctcggttt ccacacttat catcaccgtg
     1621 gtgctctgcg ttgttctgtt cctgatctgc cgctatatct taagcagacg gctgaatctt
     1681 tcataaaaat tgcattaaaa agcgccgccg cggacgaacc gaatcgtctg cggcggcgtt
     1741 ttgtatgcat gttctgcact tatgccagat gccagatatc ctcgttgtac tgtgcaatgg
     1801 tacggtcgga ggagaagaag cctgcttttg cgatgttgat caggcacttc ttgttccatg
     1861 catctttgtt ctcgtagtct ttgtatactc tttcttttgt cttgatgtac tctttcaggt
     1921 cgagcagcgt catgaaccag tctttgttca agatctcttt gtagagtctc tccaggcaaa
     1981 ccttgtcgcc gatagcaaga agctcatcgc tgacgatgaa atcaaccagc tttgcaatct
     2041 ccggatcttc gtcatagatg tcttttgagc agtaagcagc atctgcgtac aggtcgatga
     2101 catcctgcgg tttctttccg aagatgtaga tgttctcttt tccgacaaga tcgccaatct
     2161 caacgtttgc accgtcttct gttccaagcg tcagggcacc gttcagcatg aatttcatgt
     2221 ttccggttcc ggaagcctct ttggatgcca gggagatctg ctcggagata tctgctgccg
     2281 ggatcacctt tgcagctttt gatacgttgt agttctcaag cataatcact ttgaagtacg
     2341 ggcttacctc aggatcgtta tcaatcagct cctgcaggca caggatcaga tggatgatat
     2401 cttttgcgat gatataagcc ggtgcagcct ttgcaccgaa taccatggtg atcggggttt
     2461 tcggcttgtt gccgctcttg atatccatgt atttgtaaat agcgtacagg gcgttcatct
     2521 gctggcgttt gtactcatga agacgtttta tctgtacatc gatgatggaa ttttcatcca
     2581 gcgcgatatt ctgtgtctca tacaggaact gtttgagatc cagtttgcgg ttgtgtttga
     2641 tctcctcaag ttttgcaaga accttcttgt tgtctttgta tttaagcagt ttttccagct
     2701 cgtcggcatc ttttttgaat cccggtccga tcagactctc gatgtactgt gccagttcac
     2761 ggttgcagtg cagcagccag cggcggaaag tgattccgtt tgtcttgttg ttgaatttct
     2821 ccggataaat atcgtagaac ggtttcagct cggagttttt caggatctcg gtgtgcagtg
     2881 ctgcaacgcc gttaacagaa aatccgtagt ggatatccat gtgtgccata tggacgcgct
     2941 gatccttgtc gatgatcgct actttctcat ctttgtattt cttctttgcc tgtgcgtcca
     3001 gctcgtggat aatcggcatc aggtgcggaa caacctcctc gaggtaatcg atcggccatt
     3061 tttccagtgc ctctgccagg atggtgtggt tggtgtaagc acaggtcttg cttacaacat
     3121 cgatggcttc ctgcatggta aagcctctct cctgcagcag acggatcagc tccggaatga
     3181 ccatggacgg atgggtatcg ttgatctgga tcacaacgtg atcatacagc ttatgcagat
     3241 cgtagccctt ctgctcgcac tccatcagga tgaactgtgc gccgttgctg accatgaaat
     3301 actgctggta gatacgaagc agctgtccgg cacggtcgct gtcatccggg tagaggaaca
     3361 gtgtcaggtt tttctggatc tgatgcttgt caaagttgat gctgtctgat ggaacgatac
     3421 tctcgtctac cgtatctaca tcgaacagat gcagacggtt acagccattg ttgtaacccg
     3481 gtacgtcgat atcatacagg gtagaatgca tcgtgaagtt tttatacgga actgcaaagg
     3541 ataccggctg tctgacgagc cagctctcat ttgtgatcca tggatttttc acttctttct
     3601 gtttgtggtc gaccagaagc tggcggaaca gaccgaagtg ataattaagg ccgataccgt
     3661 ctcccggcag gccaagcgtt gcgatagaat caaggaagca tgctgccaga cggccgagac
     3721 ctccgtttcc aagggacggc tccatttcca tctcctcgat ctcggtcagc tctcttccgt
     3781 gagctgcaag tgctttttca acttcctcgt acatgccaag gttaatcagg ttattggaca
     3841 gcagttttcc gatcagaaac tctgctgaaa tataatacag cttcttactt ccggtgtttc
     3901 ttttgcgacc tgccatctcc tgtttggtga taaccatcag ggcttcaaag atttcgctct
//
LOCUS       DAOMUF010000182         3906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6545_length_3906_cov_3.125162, whole genome shotgun sequence.
ACCESSION   DAOMUF010000182 DAOMUF010000000
VERSION     DAOMUF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6545_length_3906_cov_3.125162"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLN77_09045"
     CDS             <1..198
                     /locus_tag="PLN77_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRM44448.1"
                     /translation="PAQRKKEMIHNPKHLVLMSAHPSPLSAYRGFFGSHPFSRTNEYL
                     EENGLAPIDWQIENVIRGAKE"
     gene            195..1820
                     /locus_tag="PLN77_09050"
     CDS             195..1820
                     /locus_tag="PLN77_09050"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRM44449.1"
                     /translation="MSKKKNTLLRNTSILMAATIISRVIGLLYRSPLGETIGNEGLGY
                     YSTASNLYTILLLISSYSIPMAVSKIVSERLALKEYRNAHRVFHGALIYAVIVGGIAA
                     LVAFFGGKFLLPYNQQNALLALRVLAPTIFFSAVLGVLRGYFQAHSTMMPTSISQIIE
                     QVFNAAFSIGAAWFFIQQFATNDTEHAKFGAAGGTLGTGAGVLAGLCFMLIVYGVNRK
                     TILRKAAKDTHHREESYREIFQVLFLMVTPVIFTTFIYNCNAYLDNYLFFTILGWHGE
                     NQKALNAAYGEFSNYYVTLINVPLAMASASASAMMPEVSSCYATGDLDEANDKILETI
                     RLTMFISIPAAVGLGVLAFPITGVLFPSSSSLSGKLLMMGAVSVVFSALSTITNSVLQ
                     SIGQQKKALHNAAISLGMDLIVLALILAVFPKTNIYAVVFAGILFSLSMCVLNNLSIR
                     KHLNFRNEFKNTYVKPLIAAAIMGVVTWVVYYGLFAITKRPSISMLIAILIAIVVYLI
                     AFVVVTGTTEAEMRRMPMGTKLVKVLRILRIYR"
     gene            complement(1924..>3906)
                     /locus_tag="PLN77_09055"
     CDS             complement(1924..>3906)
                     /locus_tag="PLN77_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HRM44450.1"
                     /translation="EAINHYNCPIVTSYAENIKNNVDELQDDSITFRNPFLAFTSEKV
                     LADGLVKEFSGLPEDEVRAAVHAGWEELGAARRDMQKKGEETLQYMKETGRRGIVLAG
                     RPYHIDPEIHHGIPDMINSYGLCVLTEDSVSHLAPLERPLRVNDQWMYHTRLYAAANY
                     VKTRDDLDLIQLNSFGCGLDAVTTDEVYEILTRSGKIYTCLKIDEVNNLGAARIRVRS
                     LLAALRAHDRKQAVREILPSSIQKPVFTKEMRKDYTILCPQMSPIHFSLLQPAFNAAG
                     YNLEVLPNDNKEAVDVGLKYVNNDACYPSLMVVGQIMQALLSGKYDLNKVAVIMSQTG
                     GGCRASNYIGFIRRALEKADMTQIPVISINLSGLEENPGFKITPDLAIRLCYAAEFGD
                     IMMKCIYRMRPYEQKKGTTDRIHKKWEKICIDFVSAKHLSHTRFKQICRTMIRDFDHI
                     PITDEKKPRVGIVGEILVKFLPAANNHLAELLESEGAEPVVPDLIDFFCYCFYNTNFK
                     VEHLGFKKSSSMLGNTGIKLINWLRSAAVAEFKKSEHFDPPADVRDLAKYASPIVSCG
                     NQTGEGWFLTGEMMELIHSDVYNIVCIQPFACLPNHIVGKGVIKAIRKEYPKANIVAV
                     DYDPGASEVNQLNRIKLMLSTANKNLKQEPNGEKDA"
BASE COUNT          923 a         1091 c          949 g          943 t
ORIGIN      
        1 ccggcacagc gcaaaaaaga gatgatccac aatccgaagc atctggtgct gatgtccgct
       61 catccgagcc cgctttctgc ataccgcgga tttttcggca gccacccgtt ttccagaacg
      121 aatgaatatc tggaggaaaa cggtcttgcg ccgattgact ggcagatcga aaatgtaatc
      181 cggggagcga aagaatgagt aaaaagaaaa atacgttact gagaaatact tctattttaa
      241 tggccgcaac gattatctcg cgtgtgatcg gcctgctgta ccggtcaccg ctcggcgaaa
      301 ccattggaaa tgagggactt ggctattaca gtacagcgag caatctgtac acgatcctgc
      361 tgctgatctc gtcctacagt attccgatgg cagtgtcgaa aatcgtatcc gagcgcctgg
      421 ccttaaaaga gtaccgcaac gcacaccgcg tcttccacgg tgccctgatt tatgcggtaa
      481 ttgtcggcgg aatcgctgca ctggtggcat ttttcggcgg aaaattcctt ctgccgtata
      541 accagcagaa tgcactcctt gccttacgcg tactggcgcc gaccatcttt ttctcggctg
      601 ttctcggcgt tctgcgcggt tatttccagg cccacagcac gatgatgccg acctcgatct
      661 cacagatcat cgagcaggta ttcaacgccg cattcagtat tggcgccgcc tggttcttca
      721 tccagcagtt cgcgacaaac gataccgaac atgccaaatt cggtgccgcc ggcggaaccc
      781 tcggaacagg agccggtgtt ctggcaggcc tctgctttat gctgatcgtt tacggcgtaa
      841 accggaaaac aattctgcgg aaagctgcca aagacacaca tcaccgtgaa gaatcctacc
      901 gtgaaatttt ccaggtcctt ttcctgatgg taacgccggt catctttacg acctttattt
      961 acaactgcaa cgcttacctg gacaactacc tgttcttcac catcctcgga tggcacggcg
     1021 aaaaccagaa agcgctgaac gctgcatacg gcgagttttc caactattac gtcaccctga
     1081 tcaacgttcc gcttgccatg gcaagtgcat ctgcatccgc catgatgccg gaggtgtcct
     1141 cctgctatgc aaccggtgac cttgatgagg caaacgacaa aatcctcgag accatccgcc
     1201 tgacgatgtt catctccatc ccggcagccg tcggccttgg cgtactcgca ttcccgatca
     1261 caggagtctt gttcccgtca agctccagcc tttccggaaa actgctgatg atgggcgccg
     1321 tttcggtcgt attctcggcc ctgtccacca tcaccaacag cgtcctgcag tccatcggcc
     1381 agcagaaaaa agccctgcat aacgcagcca tttccctcgg aatggacctc atcgtcctgg
     1441 ctctgatcct cgcagtcttc ccgaagacaa acatctatgc cgttgtcttc gcaggaatcc
     1501 tgttctcgct gagcatgtgc gtattgaata acctttcgat ccgcaaacac ctgaacttcc
     1561 gcaacgagtt caaaaacacc tacgtaaaac cattgatcgc agccgccatc atgggagtcg
     1621 taacctgggt tgtttactac ggcctctttg ccatcaccaa acgcccgtcc atcagcatgc
     1681 tgatcgcgat tctgatcgcc attgtcgtct acctgatcgc cttcgtcgtc gtaaccggca
     1741 caaccgaagc cgaaatgcgc cgcatgccaa tgggaaccaa actggtaaaa gtcctgcgta
     1801 ttctgcggat ttaccgttaa aaacaacaaa acaaaagcaa aacgaatccc cggccggaaa
     1861 gccttgataa cagctttccg gccggggatt tattttgatg ttcagaaaat atattatgta
     1921 attttacgcg tctttctcgc catttggctc ctgcttcagg ttcttattcg cagtagaaag
     1981 catcagctta atacgattga gctggttaac ctccgaagcg cccggatcgt agtcaaccgc
     2041 cacaatattc gccttcggat actccttgcg gatcgcctta ataacaccct tgccgacaat
     2101 atggttcggc aggcacgcga acggctggat gcagacgata ttgtaaacat cgctgtggat
     2161 cagctccatc atctcgccgg tcaggaacca tccctcacca gtctggttgc cgcaggagac
     2221 gatcggagaa gcatatttgg ccagatcccg tacgtcagcc ggcggatcaa aatgctcact
     2281 cttcttgaac tctgcaacag ccgcactccg tagccagttg atcagcttga tacccgtatt
     2341 tccgagcatc gaagaactct tcttgaagcc aaggtgctcc accttgaaat tcgtattgta
     2401 gaagcagtag cagaagaaat caatcagatc cggcacaacc ggctccgcac cttcactttc
     2461 gagcagctcc gccagatggt tattcgccgc cggaaggaac ttaacgagaa tctcgccgac
     2521 aataccaacg cgaggtttct tctcatccgt aatcggaata tggtcaaagt cgcggatcat
     2581 cgtgcggcag atctgtttaa agcgggtatg gcttaaatgt tttgccgaaa caaaatcaat
     2641 acagatcttc tcccatttct tgtgaatccg gtctgtcgtg ccctttttct gctcataagg
     2701 acgcatccgg taaatacatt tcatcatgat atcgccgaat tctgctgcat agcagagacg
     2761 aatcgcaaga tccggcgtga tcttgaagcc cggattttcc tccagaccgc tcaagttaat
     2821 cgagatgacc ggaatctgcg tcatatcggc cttctccagc gcacggcgga taaatccgat
     2881 gtagttcgaa gcacggcagc cgccgccggt ctggctcatg atgacagcca ctttgttcag
     2941 gtcatacttg ccggagagca gcgcctgcat aatctgtccg actaccatca gagacggata
     3001 gcaggcatcg ttgttgacgt atttcagtcc gacatcgacc gcctctttgt tgtcgttcgg
     3061 aagcacttcc agattgtatc cggctgcgtt gaacgccggc tgcagaagcg agaagtggat
     3121 cggtgacatc tgcgggcaga ggatggtgta gtctttccgc atttctttcg tgaagaccgg
     3181 tttctggatc gaggacggaa gaatctcgcg taccgcctgt ttccggtcat gtgcacggag
     3241 ggcagcgagc agagagcgga cacggattct ggcagcgccg aggttgttga cctcatcgat
     3301 cttcaggcag gtataaatct tgccgcttct ggtcagaatc tcataaacct catcggtggt
     3361 aacggcgtcg agaccgcagc cgaacgagtt cagctgaatc aggtcaagat cgtcgcgtgt
     3421 ctttacatag tttgcagctg catacagacg ggtgtggtac atccactggt cattgacacg
     3481 gagcggacgc tcgagcggtg ccagatggga aacagagtcc tccgtcagca cgcacagtcc
     3541 gtaggagttg atcatatccg ggataccgtg atggatttcc ggatcgatgt ggtatggacg
     3601 gccggcaagc acgatgcctc tgcggccggt ttctttcata tactgaagcg tttcctcgcc
     3661 ttttttctgc atatcgcgtc ttgcagcacc aagctcttcc catccggcat gaacagcagc
     3721 acggacttca tcctccggca gaccggagaa ctctttgaca agaccatcgg caagcacctt
     3781 ttcactcgta aacgcaagga acggattgcg gaacgtaatg gaatcgtcct gcagctcgtc
     3841 cacgttgttt ttgatatttt ccgcatacga ggtaacgatc gggcagttgt agtggttgat
     3901 ggcttc
//
LOCUS       DAOMUF010000183         3880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6606_length_3880_cov_3.264052, whole genome shotgun sequence.
ACCESSION   DAOMUF010000183 DAOMUF010000000
VERSION     DAOMUF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6606_length_3880_cov_3.264052"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PLN77_09060"
     CDS             <1..1343
                     /locus_tag="PLN77_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRM44451.1"
                     /translation="GENELAVKVRDETDTDWKGRGKQSLTPGGMFYTAQSGIWQSVWM
                     EWVPETYLERIVITPEYDTASVKVRVFLNGPDAGLTKKITVRESEAPDAKVICVRETR
                     ENEAELILGNFAGWSPEHPHLYGVSIEAGEDRIESYFGMRKFGIGKDEKGITRLLLNG
                     KPYFQNGVLDQGYWPESLYTPPSDEAMVYDIKTAKRLGFNMIRKHLKVECARWYSHCD
                     HIGMLVWQDMINGGGRSIQTFLLYLPTVLPFVTTEFRDNCYPLFSRTSKTGREWWKRE
                     CRETVHQLYNAPCVSLWVLFNEGWGQFDAREMTEMVRALDPTRQIDHASGWYDQGAGD
                     IKSLHNYFRPLKVKPEERAFAFSEYGGYTYPVQEHLYSEKSFGYRTYQNQAQYQKAMD
                     ALAEKIRELTEQGLAAAVYTQLTDVEEESNGILTYDRKVCKWEPQEAKDFCPKE"
     gene            1465..1971
                     /gene="ilvN"
                     /locus_tag="PLN77_09065"
     CDS             1465..1971
                     /gene="ilvN"
                     /locus_tag="PLN77_09065"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRM44452.1"
                     /translation="MEQKRTLSLLVDNTPGLLSRVAGLFSRRGYNIETITAGVTADPR
                     YTRMTVVLYCSDEMILEQIKKQLAKLVDVRDIKELKPDESVRRELMLVKIRVNENERR
                     NVIAISDVFRGRIVDVGQESLIVELTGNQSKLEGFLNLVQGYEILELARTGVTGLSRG
                     VHDVRYLD"
     gene            2130..3149
                     /gene="ilvC"
                     /locus_tag="PLN77_09070"
     CDS             2130..3149
                     /gene="ilvC"
                     /locus_tag="PLN77_09070"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRM44453.1"
                     /translation="MAARLFYQEDCNLSLLEGKKIAIIGYGSQGHAHALNLKDSGCDV
                     IIGLYEGSKSWAKAEAQGFKVYTAAEAAKQADIIMILINDEKQAAMYKKDIEPNLEEG
                     NMLMFAHGFAIHFGQIVPPKNVDVAMIAPKAPGHTVRSEYQAGKGTPCLVAVEQDYTG
                     KALDRALAYGLGIGGARAGLLETTFRTETETDLFGEQAVLCGGVCALMQAGFETLCEA
                     GYDPRNAYFECIHEMKLIVDLIYQSGFAGMRYSISNTAEYGDYITGPKLITEETKKTM
                     KKILSDIQDGTFAKDFLLDMSPAGGQAHFRAMRRKAAEHPSEKVGEEVRKLYSWSNED
                     KLINN"
     gene            complement(3307..3663)
                     /locus_tag="PLN77_09075"
     CDS             complement(3307..3663)
                     /locus_tag="PLN77_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HRM44454.1"
                     /translation="METEIYFLLHSLGIGAKYRGFRYLAYGIALCMEDEDYLLRVSKT
                     LYPKIAQTFQVSSSCVERDIRTAISVCWTRGNRDLLFSLSVHPVLTKPTNSEFFDILS
                     SYIKYYRAFPACRQEA"
BASE COUNT         1128 a          826 c         1057 g          869 t
ORIGIN      
        1 cgggagagaa cgagcttgcc gtcaaggtgc gcgatgagac ggacacggac tggaagggcc
       61 gcggaaagca gagccttacc ccgggcggca tgttttacac cgcgcagagc ggcatctggc
      121 agagcgtctg gatggaatgg gtgccggaga cgtatctgga acgcatcgtg atcacgccgg
      181 aatatgatac ggcgagcgta aaagtacggg ttttcctcaa cggaccggat gcgggactga
      241 cgaaaaaaat tacggtgcgg gagagcgaag cgccggatgc gaaggtgatc tgcgtccggg
      301 agacgagaga aaacgaggcg gagctgattc tcggtaattt tgcaggctgg agcccggaac
      361 atccgcatct ctatggggtt tccattgaag cgggagaaga ccgcatcgag agttatttcg
      421 gcatgcgcaa attcggcatt ggaaaagatg agaaaggaat cacgcgtctt ctcttaaacg
      481 gaaagccgta tttccagaac ggtgtcctcg accagggtta ttggccggaa agcctctaca
      541 cgccgccgtc cgatgaggcg atggtttatg acattaagac ggccaaacgc ctcggtttca
      601 atatgatccg gaaacacctg aaagttgagt gcgcaagatg gtattcccac tgcgaccata
      661 tcggtatgct ggtgtggcag gatatgatca acggcggcgg ccggtcgatc cagacgttcc
      721 ttttgtacct tccgaccgta ctgccgtttg tgacaacgga attcagggat aactgttatc
      781 cgctcttttc gcggacctca aaaacgggaa gagagtggtg gaagcgggag tgccgcgaga
      841 cggttcatca gctttacaat gcaccgtgcg tgtcgctctg ggttctgttc aacgagggat
      901 ggggacagtt tgatgccagg gagatgacag aaatggtgcg cgctctcgat ccgacgcggc
      961 agatcgacca cgcgagcggc tggtacgatc agggagcggg ggacatcaag agccttcaca
     1021 attactttcg tccgttaaaa gtaaaaccgg aggagcgcgc gtttgctttt tctgaatacg
     1081 gcggctatac gtatccggta caggagcatc tttactccga aaaatcgttc ggctaccgca
     1141 cataccagaa ccaggcacag taccagaaag cgatggatgc actggcagaa aagataagag
     1201 aactcacaga acagggactt gccgcagcag tctacacaca gcttaccgat gtcgaggagg
     1261 agtcgaacgg catccttacg tatgaccgga aggtatgcaa atgggaacca caggaggcaa
     1321 aagatttttg cccaaaagaa taatttggta aaatttttgt tgacaaagcg aggataagtt
     1381 gctaatatat tggataaggc gttggaagtt gtaagaaatt gctgaaaatc cgcggcgcct
     1441 gcggatggag gaaagttggt gtttatggaa cagaaaagaa cattatccct gctggtggat
     1501 aatacgccgg gacttttaag cagagtagct ggtcttttca gccgacgcgg atacaatatc
     1561 gagacgatca ccgccggtgt gacagcagac ccgcgataca caagaatgac tgttgttctg
     1621 tactgcagtg atgaaatgat tctggagcag atcaaaaaac agctggcaaa acttgtcgat
     1681 gtgcgggata tcaaagagct gaagccggac gagagtgtca gacgagagct gatgctggtc
     1741 aaaatccgtg tgaatgaaaa tgaacgccgc aatgtcatcg ccatctccga tgtcttccgt
     1801 ggaagaatcg tggatgtggg acaggaaagc ctgattgtag agctgaccgg aaaccagtca
     1861 aagctcgaag ggttcctgaa tctggtacag ggatacgaaa tcctggaact ggccagaaca
     1921 ggagtgacag gactctccag aggagtccac gatgtgcgtt acctcgatta acgcagatca
     1981 cattacatac gtttgctgga ggaatgaaat ttttgtatag aaaagttcat ttccctgaca
     2041 aacagcaaaa ggcagaaatg cttttcgctg tataaattga ccgacctgcc gccggtcaag
     2101 acattctata tattatggag gaaaataaaa tggcagcaag acttttttat caggaagatt
     2161 gtaatctctc tttactggaa ggcaaaaaaa tcgcaattat cggctacgga agccagggac
     2221 atgctcatgc actgaacctg aaagattccg gctgtgatgt tattattgga ctttatgaag
     2281 gcagcaaatc ctgggcaaaa gcagaggcac agggattcaa agtatatacc gctgctgagg
     2341 ctgcaaaaca ggctgatatc atcatgatcc tgatcaacga tgagaaacag gctgcaatgt
     2401 acaaaaaaga tatcgagcca aacctggaag agggcaacat gctgatgttc gctcatggtt
     2461 tcgctatcca cttcggacag atcgtacctc cgaagaacgt agatgtagct atgatcgctc
     2521 cgaaagcacc tggccacaca gtaagaagcg agtatcaggc tggcaaagga actccttgcc
     2581 tggttgctgt tgagcaggat tacaccggaa aagctctgga cagagcactg gcttacggcc
     2641 ttggaatcgg cggagcaaga gctggtcttc tggagacaac cttcagaact gagaccgaaa
     2701 cagacctgtt cggtgagcag gcagttctct gcggcggtgt atgcgccctg atgcaggctg
     2761 gatttgagac tctgtgcgaa gccggatacg acccgagaaa tgcatatttt gagtgtatcc
     2821 atgagatgaa actgatcgta gatctgatct accagtccgg atttgcagga atgagatatt
     2881 ctatctccaa tacagcagag tatggcgatt acatcaccgg accgaagctg attacagaag
     2941 agacgaagaa gaccatgaag aaaatcctct ccgatattca ggatggtact tttgcaaaag
     3001 atttccttct ggatatgtcc ccagcaggcg gacaggctca cttccgtgct atgagaagaa
     3061 aagctgcaga gcatccgtca gagaaagtcg gagaagaagt cagaaaactg tacagctgga
     3121 gcaacgagga caaactgatc aacaactaat cggtttgctg tcaaatgatc catatcggga
     3181 ggctgccttt gcgggcagtc tccttttttc gttctatcgt tttatcatag aaaaaacact
     3241 tccgcgccat gcatatcccc caatatgata gggcgcggaa gtgttatctt ttctgtattc
     3301 gtttttttat gcctcctgac gacaggcagg aaaagcacgg tagtatttga tataagaact
     3361 taaaatatca aaaaattcgg aattggtcgg ttttgtcaga accggatgca cagaaagcga
     3421 aaagagcaga tcacggtttc cgcgggtcca gcagacggaa atagcggtgc ggatgtctcg
     3481 ttcgacgcag gaggacgata cctgaaacgt ttgcgcgatc tttgggtaca gcgttttgct
     3541 gacccgaagg agataatctt cgtcttccat gcataaagcg ataccatagg cgagatagcg
     3601 aaatccgcgg tattttgcac caattcctaa agagtgtagt agaaagtaaa tttcagtttc
     3661 cattacgatg tctcctttta ttcagttttt acatagtctt tgcagacggg catgccagag
     3721 gtgaaagctt gtatggcata cgattttccc ctttcactta tgatacgtgg aaaacgtcga
     3781 aaaattacat attaagaaag aaaaaagtga cgatatagta aaataaaaga gaatacgaca
     3841 cgaaataaag aaacgaatgt aatgaaacgg gagagggaaa
//
LOCUS       DAOMUF010000184         3878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6613_length_3878_cov_2.679309, whole genome shotgun sequence.
ACCESSION   DAOMUF010000184 DAOMUF010000000
VERSION     DAOMUF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6613_length_3878_cov_2.679309"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLN77_09080"
     CDS             <1..292
                     /locus_tag="PLN77_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HRM44455.1"
                     /translation="SFEFIEGESLLIMLDMKGICASSGSACTSGSLDPSHVLLAIGLP
                     HEKAHGSLRLTLSEEITKEDVDFVVEQIKAIVDRLRSMSPLYEDYIKKQQGK"
     gene            400..837
                     /gene="nifU"
                     /locus_tag="PLN77_09085"
     CDS             400..837
                     /gene="nifU"
                     /locus_tag="PLN77_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HRM44456.1"
                     /translation="MYSEKVMDHFQHPRNVGEIENASGVGTVGNAKCGDIMRMYLDID
                     DNGIIQDCKFKTFGCGAAVATSSMATELVKGKTIQEALEVTNKAVMEALDGLPPVKVH
                     CSLLAEEAIHAALWDYAEKHHIKIEGLAKPKSDISEGEEEEEY"
     gene            840..1934
                     /gene="mnmA"
                     /locus_tag="PLN77_09090"
     CDS             840..1934
                     /gene="mnmA"
                     /locus_tag="PLN77_09090"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRM44457.1"
                     /translation="MGKKKVVVGMSGGVDSSVAAYLLKKAGYDVIGVTMQIWQDEAET
                     VQEENGGCCGLSAVDDARRVAQVLDIPYYVMNFKKEFRDNVMDYFTNEYLCGRTPNPC
                     IACNRYVKWEALLQRSLSIGADYIATGHYARIEQLANGRYAIANSVTAAKDQTYALYN
                     LTQEQLSHTLMPVGDYTKDQIREIAAKIGLPVAKKKDSQEICFVPDQDYASFIQNETG
                     IVAPKGNFVNTKGEILGTHEGITHYTVGQRRGLGLPMGHRVFVLEIRPETNEVVVGEN
                     EEVFAKVVKANKVNYMAIPPLELGEELSCTAKIRYGHKGSPCVIKRTGEDEITCTFPD
                     GVRAPTPGQAVVFYVDGCVGGGGTITEAER"
     gene            1931..2731
                     /locus_tag="PLN77_09095"
     CDS             1931..2731
                     /locus_tag="PLN77_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRM44458.1"
                     /translation="MKIADCHMHSFFSSDSEAPTEEMVKRAVELGLPAICLTDHYDMD
                     YSTGEFQLDTPAYAAKIRELQEKYHDRIDIRFGVELGLQLHLKERMEEYVNAWPFDYV
                     IGSMHVIDGKDPYYEDAFPDWTAEELYRAYFRATAENIRFFHNFQTLGHLDYVVRYCR
                     EKGKDYSYRAFADEIDTILKELIQYDIALEINTGGYKAGLGVPNPTPDVIRRYRELGG
                     EKITFGADAHKPEHIAFHFADAAAIAQEAGFRYYVRFKEKKPEYLPIQ"
     assembly_gap    2778..2787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3007..>3878
                     /locus_tag="PLN77_09100"
     CDS             3007..>3878
                     /locus_tag="PLN77_09100"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRM44459.1"
                     /translation="MAVKVAINGFGRIGRLAFRQMFGAEGYEVVAINDLTSPKMLAHL
                     LKYDSSQGKYALADTVTSDEDSITVDGKNIKIYAFPDANNCPWGELGVDVVLECSGFY
                     TSKAKAQAHINAGARKVVISAPAGNDLPTIVYNVNHETLTADDNIISAASCTTNCLAP
                     MAKALNELAPIKSGIMVTIHAYTGDQMTLDGPQRKGDLRRSRAAAVNIVPNSTGAAKA
                     IGLVIPELNGKLIGSAQRVPTPTGSTTILTAVVEGNVTVDQINAAMKAASNESFGYNE
                     DQIVSSDIVGMRFGS"
BASE COUNT         1146 a          872 c          999 g          851 t
ORIGIN      
        1 cagctttgaa ttcatcgagg gagagtccct tctgatcatg ctggatatga aaggtatctg
       61 cgcatccagc ggttctgcat gtacttccgg atccctggat ccgtcccatg tactgctggc
      121 catcggactg ccgcacgaga aagcacacgg ttccttacgt ctgactttaa gtgaggagat
      181 cacaaaagag gacgttgatt tcgttgtaga gcagatcaaa gcgatcgtag accgtctgcg
      241 cagcatgtcc ccgctgtatg aggattacat caaaaaacag cagggtaaat aagtttaaaa
      301 aaataagaca gtccatccag aaagccggta cagaaaaatt caggtaaaag ctgtgccgga
      361 ggaggactgc tttgaaatag ttcaggagga agaataacaa tgtacagtga aaaagtaatg
      421 gatcatttcc agcatccgag aaatgtagga gaaattgaga acgcaagcgg tgtaggtacc
      481 gtaggtaatg caaaatgtgg agatatcatg agaatgtatc tggatatcga tgacaacggt
      541 atcattcagg attgcaaatt caaaacattc ggttgtggtg cagctgtcgc aaccagcagt
      601 atggcaaccg agctggtaaa aggaaaaacc atccaggaag ctctggaagt aaccaacaaa
      661 gccgttatgg aagcactcga cggacttccg ccggtcaaag ttcactgttc tctgcttgca
      721 gaagaagcaa ttcacgctgc actctgggat tacgcagaga aacaccacat caagatcgaa
      781 ggtcttgcaa aaccgaagtc tgatatcagc gagggcgagg aagaggaaga atactaagta
      841 tgggaaaaaa gaaagtagtc gttggaatgt ccggcggcgt agattcctcg gtggcagcgt
      901 atctcctgaa aaaagcagga tacgatgtca tcggggtgac catgcagatc tggcaggatg
      961 aggccgagac agtccaggag gaaaacgggg gctgttgcgg cctttctgct gttgatgatg
     1021 caaggcgcgt ggcacaggtg ctggacattc cgtattatgt catgaacttc aaaaaagagt
     1081 tccgtgataa cgtcatggac tatttcacga acgaatatct ctgcggcaga acaccgaacc
     1141 cgtgtatcgc ctgcaaccgc tatgtgaaat gggaagcgtt gcttcagcgc agtctttcca
     1201 ttggagcgga ctacattgcg accggacatt atgcaagaat cgaacagctt gccaatggac
     1261 ggtatgcgat tgcaaattcg gtaacagcgg caaaagacca gacgtatgca ctgtataatc
     1321 tgacccagga gcagctttcg catacgctga tgccggtcgg agactacaca aaagatcaga
     1381 ttcgtgagat tgcagcaaaa atcggtcttc cggtagcaaa aaagaaggat tcccaggaaa
     1441 tctgcttcgt gccggatcag gattacgctt cctttatcca gaacgagaca ggaatcgtgg
     1501 caccgaaagg caatttcgtg aatacaaagg gtgaaatcct cggtacccat gaggggatta
     1561 cccattacac cgtaggacag cgccgcggac tcggccttcc gatgggacac cgcgtgttcg
     1621 tactggaaat ccgtccggag acgaacgagg ttgtggtcgg cgaaaatgaa gaggttttcg
     1681 caaaagtagt aaaagcaaac aaagtaaact atatggcaat tccgccgctg gaacttggag
     1741 aagagctctc ctgcaccgca aaaatccggt acgggcacaa aggctccccg tgcgtgatca
     1801 aacggaccgg ggaagatgag attacctgta ccttcccgga cggcgtgcgt gctccgacac
     1861 cgggacaggc tgttgtcttc tacgtggacg gctgtgtcgg cggaggcgga acgattacgg
     1921 aggccgaaag atgaaaatag cggactgtca tatgcacagt ttcttttcaa gtgacagcga
     1981 agcaccaaca gaagagatgg taaaacgggc agtcgagctg ggacttccgg caatctgcct
     2041 gacggatcac tacgatatgg attacagtac cggcgagttc cagctcgata caccggcata
     2101 tgcggcaaaa atccgggaac tgcaggaaaa ataccacgac agaattgata tccgattcgg
     2161 cgtggaactg ggactgcagc tccacttaaa agaacggatg gaagagtatg taaatgcgtg
     2221 gccgttcgac tatgtgatcg gctccatgca tgtcattgac ggaaaagacc cctattatga
     2281 ggatgctttc ccggactgga cagcagaaga gctgtaccgg gcgtattttc gggctacagc
     2341 agaaaatatc cggttcttcc acaatttcca gacgcttggt catctggatt atgtggtacg
     2401 ctactgcaga gaaaagggaa aagattacag ctaccgcgca tttgcggacg agatcgatac
     2461 cattttaaaa gagctgattc agtacgatat cgcgctggaa atcaacaccg gcggctacaa
     2521 ggcaggcctt ggcgtgccga atccgacgcc ggatgtcatc agaagatacc gggaactggg
     2581 cggcgaaaag atcaccttcg gtgccgatgc ccacaaaccg gaacatatag cgtttcattt
     2641 cgcggacgcg gcagccatcg cgcaggaagc tggattccgg tactacgtgc ggtttaaaga
     2701 gaagaagccg gaatatctgc cgatccaata gaaaacgcgc aggaaaagcc ggaaaaaagc
     2761 cccggaaagg ctggatgnnn nnnnnnntcc ggcttcttct gcaatagacg gatctttcga
     2821 aattttgcag aaatacggat tggcatccgg cttcttcaca aattttagaa ataatttcgt
     2881 attttcttga aaatagaact tgaattttcc gacgaaatta ggtattatac agataaggtt
     2941 gatgtttctt taacaaagta cagagactca aaattaaaat tcacttttta ggaggaattg
     3001 aaaatcatgg cagtaaaagt agcaatcaat ggttttggcc gtatcggacg tcttgcattc
     3061 agacagatgt ttggagcaga aggttatgaa gtagtagcaa tcaacgattt aacatccccg
     3121 aagatgttag ctcatctgtt aaaatatgac tcttctcagg gaaaatatgc tctggctgat
     3181 accgtaacat ccgatgaaga ttccatcaca gtagatggca aaaacatcaa aatctatgct
     3241 ttcccggatg ctaacaactg cccatgggga gagcttggcg tagacgtagt tctggagtgc
     3301 tccggattct acacctccaa agcaaaagct caggcacaca tcaatgcagg cgctcgtaaa
     3361 gtagttatct ccgctccggc tggtaacgac cttccgacca tcgtttacaa tgtaaaccat
     3421 gagaccctga cagcagacga taacatcatc tccgctgcat cctgcaccac aaactgcctt
     3481 gcacctatgg caaaagctct gaacgaactg gctccgatca aatccggtat catggttact
     3541 atccatgctt acactggaga tcagatgact ctggacggac cgcagagaaa aggcgatctg
     3601 agaagatccc gtgcagctgc agttaacatc gttccaaaca gcacaggtgc tgcaaaagct
     3661 atcggtctgg ttatcccgga actgaacggc aaactgatcg gttctgcaca gcgtgttcca
     3721 actcctacag gatccacaac tatcctgaca gcagttgttg aaggtaacgt aacagttgat
     3781 cagatcaacg cagctatgaa agcagcttct aacgagtcct tcggatacaa cgaagatcag
     3841 atcgtttcta gcgatatcgt aggaatgaga tttggttc
//
LOCUS       DAOMUF010000185         3852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6676_length_3852_cov_4.265736, whole genome shotgun sequence.
ACCESSION   DAOMUF010000185 DAOMUF010000000
VERSION     DAOMUF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6676_length_3852_cov_4.265736"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..955)
                     /locus_tag="PLN77_09105"
     CDS             complement(80..955)
                     /locus_tag="PLN77_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM44460.1"
                     /translation="MQTKLVLLGTGTPNACPNANGPSSAVVVGDRAYLVDFGPGVVRQ
                     ASAAYFNGVDALRPDLLTVAFCTHLHTDHTAGYPDLIFTPWVLERPVPLKVFGPKGLQ
                     HMTDHILQAYETDIDFRIHGFEKANESGYRVEVTEIESGIVYRDDLVTVEAFPVSHGT
                     LECYGYKFTTPDRTIVITGDTAPLDLVAEKSKGCDILLHEVEYAAGISCREPKWQKYH
                     REVHTLSTDLAGVAKKANPKLLVTYHRIYHMNVQDNHKNLAAEMAWRDTAILDEIRDA
                     GYDGYVVNGKDLDVF"
     gene            complement(1029..2444)
                     /gene="nhaC"
                     /locus_tag="PLN77_09110"
     CDS             complement(1029..2444)
                     /gene="nhaC"
                     /locus_tag="PLN77_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HRM44461.1"
                     /translation="MSGTKEKKLNLPQSLLLLLLIIVSVAVCIRLKTGGPMIGLFASW
                     IFIYLFCKIFGISYANIVAGAYDAIRMVVPTLCLLMAIGVMIGTWLQSGTIATIISWG
                     LKMINPSWLLPLTLLFCSILSIVTGTSYGSVGSAGVAMMAIGNAMGIHPGMVAGAVIC
                     GAMFGDKLSPLSDTTNLAPAVADAKLGDHIRSMLWTTLPTYVITLILFTILGFQQTSG
                     SYTEGSITVYIDALNGEFQLGWITMIPAILIIVLLLCKVNAISALGLSSFAAGFVSYF
                     VQHDTLQGIIRTAYNGYTTTIEEPVLQSILNRGGMGSMLQYVAIICFAVGMGGMLEKL
                     GVLDHILQAIVKRINSDGSMILATMIVGYVTSLISCSQPMAHVLTGRLMAPVFQERKV
                     APEILSRCLEDSGTMAGPMIPWHGYGVYMAGTLGVAWSAFFPYLFLLYLTPVFSIFYG
                     FTGISIKHLPETKNNESKEEK"
     gene            2671..3852
                     /locus_tag="PLN77_09115"
     CDS             2671..3852
                     /locus_tag="PLN77_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM44462.1"
                     /translation="MTRIDIISGFLGAGKTTLIKKLLGDALKGQQVVLIENEFGEIGI
                     DGGFLKDAGIEIREMNSGCICCSLVGDFGAALKDVITKYHPDRIIIEPSGVGKLSDVI
                     KAVDGVEKEAGVALNSATTVVDVMKCKMYLRNFGEFFENQVKSAGTIILSRTDKADTE
                     KVEAAVKMLRELNPEAHIITTPVEVLGGKKVLDTMEGAIINLAQVEEEEHHHHHDHDE
                     CCCGHDHDEEHEHHHHDHDEDEHEHHHHDHDGECCCGHDHDEEHEHHHHDHDDECGCG
                     HDHEHEHHHHDDECGCGHEHHHHHHADEVFTSWGQETVKTFTKEQIEGILKKLSEDTA
                     YGMVLRAKGMVAGADGQWIYFDMVPEEYEVRDGAPEFTGRICVIGSKLAEDKLAELFG
                     L"
BASE COUNT         1061 a          897 c          989 g          905 t
ORIGIN      
        1 tcctgataca agcaagtccc aacccactgc ttattgcttc acgcaataga agcagtgggt
       61 tgggacttgc ttgattcggt taaaatacat ccaggtcttt tccgttcacg acatatccgt
      121 cataccctgc atcccggatt tcatccagta tcgcagtatc gcgccatgcc atctcggcgg
      181 caaggttttt gtggttatcc tgtacattca tatggtaaat ccggtgatac gtaacgagca
      241 gtttcggatt tgcctttttt gcaacaccgg caagatccgt gcttaacgta tgcacttccc
      301 ggtggtattt ctgccatttt ggttcgcggc aggagattcc ggcggcgtat tccacttcgt
      361 gcagcaggat atcacagcct ttcgattttt ctgccacgag atccagcgga gcggtatcgc
      421 cggtgatgac gattgtgcgg tccggtgtgg tgaatttata gccgtagcat tccagtgttc
      481 cgtggctgac cggaaaagcc tccaccgtaa ccaggtcatc ccggtagaca atgccggatt
      541 caatttctgt tacctcaacg cggtagccgg attcgtttgc tttttcgaaa ccgtgaattc
      601 ggaaatcaat gtcggtttca taggcctgta agatatgatc ggtcatgtgc tgtaagccct
      661 ttggcccgaa aactttcaat ggaaccgggc gctccagcac ccacggagta aaaatcagat
      721 ccgggtatcc tgcggtatgg tctgtgtgaa ggtgcgtaca gaaggcaacg gtcagaagat
      781 ccggccgcag ggcatccacg ccgttaaaat aggcggctga ggcctggcgg acaacgccag
      841 ggccaaaatc taccagataa gcacggtcac cgaccacgac agcggaggaa ggtccgttgg
      901 cattcggaca cgcatttggt gtgccggttc cgagaagaac aagttttgtc tgcattcttt
      961 ctcttttcct tttctttttt tgtttctatt tatgtttatg atttcctgtt acgatttctg
     1021 cttttgcctt atttctcttc ttttgattca ttgtttttgg tttccggaag atgcttaatg
     1081 gaaattccgg taaatccata gaaaatactg aaaaccgggg tcaggtaaag caggaataag
     1141 tacgggaaaa atgccgacca tgcaacacca agtgtgccgg ccatgtatac gccgtagcca
     1201 tgccatggaa tcataggacc agccatagtt ccggagtctt cgaggcagcg ggaaagaatt
     1261 tccggtgcaa ccttgcgctc ttgaaagaca ggtgccatca gacggccggt cagaacgtgt
     1321 gccatcggct gggagcagct gatgaggctg gtcacgtaac cgacaatcat cgttgccagg
     1381 atcatacttc catcgctgtt gattctcttt acaatcgcct gaagaatgtg atccagtaca
     1441 ccgagttttt ccagcatgcc gcccatgccg acagcaaagc agataatggc tacgtactgg
     1501 agcatgcttc ccatgccgcc gcggtttaag atggattgta ataccggttc ttcgattgtg
     1561 gttgtgtaac cgttgtaagc tgtgcggatg atgccctgca gagtatcgtg ctgcacaaaa
     1621 taggaaacaa atccggcagc gaagctggaa agaccaagtg cagagatcgc atttactttg
     1681 cagaggagca atacaataat caggatggca gggatcatgg taatccatcc gagctggaat
     1741 tcaccgttta aagcatcgat gtaaacggta atgcttccct cggtatagct tccgctggtc
     1801 tgctggaaac cgagaatagt aaaaagaatt aaggttatca catatgttgg aagggtggtc
     1861 cagagcatcg agcggatatg atcgccaagt ttggcatctg caacggccgg agcaaggttt
     1921 gtggtatcgg aaagaggact taatttgtca ccgaacatgg caccgcagat gacagcaccg
     1981 gctaccatgc caggatggat acccatggcg tttccgatcg ccatcatggc aacacctgcg
     2041 cttccgacgg aaccatagga agtaccggtt acaatactca aaatggagca gaacagcaac
     2101 gtcagaggca gcagccagga cgggttaatc atttttaagc cccaggaaat gatggtggca
     2161 atggtgccac tctgcagcca ggtgccgatc atgacaccga tggccatcag aaggcagagc
     2221 gtcgggacga ccatacggat ggcatcgtat gcgccggcaa caatgttggc gtaagaaata
     2281 ccgaaaatct tacaaaacag atagataaaa atccagcttg caaacaggcc gatcatagga
     2341 ccgccggttt tcaggcggat acagactgcg acggatacga tgatcagcag cagcaaaagc
     2401 agtgactgtg gcagattgag ttttttttct ttcgttccac tcataaaaaa atcccccatt
     2461 cataggtaaa atgatttgtc cattcaacgc gttaaagtag aaaagacatg taatatatta
     2521 taatggtgaa atgcagaaaa agcaagggaa attctcttga ttttttgttc tatggttgct
     2581 ataataagcg tgagtgtggg gcggaggata aacttcggcc cttttgcagc taactataaa
     2641 cggaatcaac aagataaaga ggttaaatca atgacaagaa tagacattat ttcaggtttc
     2701 ctgggcgcag gaaaaacaac cctgatcaaa aaactcctcg gagacgcact gaaaggccag
     2761 caggttgttc tgatcgagaa cgaattcggt gaaatcggaa tcgacggagg cttcttaaaa
     2821 gatgccggta tcgaaatccg tgaaatgaac tccggctgca tctgctgctc tcttgtggga
     2881 gacttcggcg cagcattaaa agacgttatc accaaatatc acccggaccg catcatcatc
     2941 gagccgtccg gcgtaggtaa actctccgac gtcatcaagg ctgtagacgg tgtagaaaaa
     3001 gaagcaggcg tagccctgaa ttccgcaaca accgtagtcg acgttatgaa atgcaaaatg
     3061 tatctcagaa acttcggcga attcttcgag aatcaggtaa agagcgcagg aaccatcatc
     3121 ctgagccgta ccgataaagc cgatacggaa aaagtagagg cagcagtaaa aatgctccgc
     3181 gaactgaatc cggaagcaca cattattacc acaccggttg aagtactggg tggtaagaaa
     3241 gtactcgata cgatggaagg tgccatcatc aaccttgcgc aggtagaaga ggaagagcat
     3301 catcatcatc atgaccacga cgagtgctgc tgcggccatg accacgatga ggagcatgag
     3361 caccatcatc acgatcatga tgaggacgaa catgaacatc atcaccacga ccatgacggc
     3421 gagtgctgct gcggccacga tcacgacgag gagcatgagc atcatcacca cgaccatgat
     3481 gacgagtgcg gctgcggcca tgatcacgaa catgagcatc atcatcacga tgacgagtgc
     3541 ggctgtggtc atgagcatca tcaccatcat cacgcagacg aagttttcac aagctggggt
     3601 caggagactg tgaagacctt caccaaagag cagatcgaag gcatcttaaa gaaactttcc
     3661 gaggataccg cttatggtat ggttcttcgt gccaaaggta tggtagcagg cgccgatgga
     3721 cagtggatct atttcgatat ggttccggaa gagtacgaag tacgtgacgg agcaccggaa
     3781 ttcaccggaa gaatctgtgt catcggttcc aaattagcag aagataagct ggcagagctg
     3841 ttcggactgt aa
//
LOCUS       DAOMUF010000186         3848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6684_length_3848_cov_2.650672, whole genome shotgun sequence.
ACCESSION   DAOMUF010000186 DAOMUF010000000
VERSION     DAOMUF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6684_length_3848_cov_2.650672"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLN77_09120"
     CDS             complement(<1..254)
                     /locus_tag="PLN77_09120"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HRM44463.1"
                     /translation="MKKKDLILAGAVLLLAGILALALRLPKTENGNTMKVTVDGEVYG
                     TYSLAENQTVKIQTGHGTNVLVIENGNVHMEEADCPDGYC"
     gene            complement(275..1147)
                     /locus_tag="PLN77_09125"
     CDS             complement(275..1147)
                     /locus_tag="PLN77_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM44464.1"
                     /translation="MKNLDLSTLPAKKRKQIVSCLRAGIQLLYFLFLPSVFTAAFNGV
                     KYIFTQLGQGEKIEMTAFVSALLLVCAFTIVFGRFFCGFACAFGSLGDAMHAIYGIIC
                     KKRKKKPRCIKPELAKKLVLIKYGILLLIALCCFAGVYQKARGTSPWDVFSMLHAGNF
                     RLSSYIPGIVLLVLILVGMFFEERFFCRFLCPMGAVFSLLPILPFFTLHRKRENCIRG
                     CSACTRQCPSAVELPSDGYVEISGDCFQCQKCIDTCPKGNIRCGVKEFHGNEILFTLF
                     RAVFLLTLLLWAGI"
     gene            complement(1149..1637)
                     /locus_tag="PLN77_09130"
     CDS             complement(1149..1637)
                     /locus_tag="PLN77_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM44465.1"
                     /translation="MRQLIVRFLNVAVIAAVLIGYNTVLDKRDKEDQIARLSAELETT
                     QLQKENLQAAAAASNTADTAASEAAEAEGQYVDGTYDGEADGFGGTIAVEVTVEGGQI
                     TDLAITSADGEDSAYLSNAEAIIPKIIEAQSADVDTISGATFSSTGIRNAAQEAIEKA
                     EK"
     gene            complement(1650..3707)
                     /locus_tag="PLN77_09135"
     CDS             complement(1650..3707)
                     /locus_tag="PLN77_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM44466.1"
                     /translation="MGKWKKLTAFAPALAAVIVAACAGISLNGYTAPVYAVEQPEALV
                     TPEPEQDTDAAEKKDDTKKKDDTKKTTDTEAELVAAVGSGSFELADGTYEGSGTGFGG
                     TITVSVVLKDKTIQSISIVSAPGEDAAFLNRAKGVIDRIIASQSVDVDTVSGATFSSK
                     GILAAVKNALTGQEDTSEPASASGAAGGGASAGGTVTVSKIEEPAAYRDGTYYGTGTG
                     FGGTMKVCVVVSGGKIASIDIVENSDTPSYLSSASSLISTIISTQSTNVDTVSGATYS
                     SRGIIEAVRSALSQAAVNGSSSTNGGSQSNTNNGNNSQNNNQNNNNSSASKGSFPYQD
                     GIYYGTAAGFQGDIKVAVALQDQTIKAVLILENEDDETFFNRAKVVADRIVDGQKTDV
                     DLVSGATYSSRGIQNAVKQALENAKKATNGETVPDNGTSSDGTTTIPEGKFPYEEGIY
                     YGTGEGYLGDITTAVVIQDETIKAILVTESEDDEAFLKRAKQTAKDVVKNQTLEVDTV
                     SGATYSSRGILAAIEEALKEAERVTEEKKNPTPTPTVTPTPTETPTPTETPEPTETPT
                     PTPTETPEQKIYQDGEYTAIAVCEPDEDEDFTAYQLSVTVTIENDKIVALSNITGDGD
                     SQNASYIRRASEGTSKIKGVSAQILETGTLENIDTVSRATCSSKAILEACRNALDAAK
                     VTQ"
BASE COUNT          801 a         1024 c          925 g         1098 t
ORIGIN      
        1 ttgcagtagc cgtccgggca gtcagcttct tccatatgga catttccgtt ttcaatcacc
       61 aggacattcg ttccgtgacc cgtctgaatt ttcacggtct gattttctgc gagtgaatac
      121 gttccgtaga cctcgccgtc caccgtgact ttcatggtat ttccattttc cgttttcggc
      181 agccgaagcg ccagggcaag gattccagcc aggagcagaa cggcgcctgc cagaatcaga
      241 tcttttttct tcatatagtt ttccttattc tgaatcagat gccagcccat aacagcagtg
      301 tcagcaggaa tacagcacgg aacagcgtaa agaggatttc atttccgtga aattccttta
      361 ccccgcagcg gatattacct ttcggacacg tatcaataca tttctggcac tggaaacagt
      421 cgccggagat ctccacgtat ccatcggatg gcagttccac cgctgacggg cactgtctcg
      481 tgcaggcgct gcagccgcgg atgcagtttt cgcgtttccg gtgcagagta aagaacggca
      541 ggatcggaag cagagaaaag acagctccca ttgggcacag gaaacggcag aaaaagcgct
      601 cctcaaagaa catgccgacc aggatcagca ccagaagaac aatgcctgga atataagagg
      661 aaagtcggaa atttcccgca tgcagcatgg aaaagacatc ccacgggctt gttcctcttg
      721 ctttctgata aactcctgca aagcagcaaa gggcaatcag aagcagaatg ccgtatttga
      781 tcagtaccag tttttttgcc agctccggtt tgatgcatct gggcttcttt ttacgtttct
      841 tacagattat accataaatt gcgtgcattg catcccccag acttccaaaa gcgcatgcaa
      901 aaccgcagaa aaatcttccg aatacgatcg taaatgcaca gacaaggagc agagccgaca
      961 caaacgcggt catctcaatt ttttcaccct gtccaagctg cgtaaaaatg tatttcactc
     1021 cgttaaatgc agcggtaaat actgacggaa gaaacaggaa atataaaagc tggattccgg
     1081 cacgcaggca ggaaacgatc tgttttctct tctttgccgg taatgtggat agatctaaat
     1141 ttttcatgtt atttctccgc cttttctatt gcttcctgcg ccgcatttct gataccggtg
     1201 gagctgaatg ttgctccgct gatcgtatca acatcggcgg actgtgcctc aataatcttt
     1261 ggaatgatcg cctccgcgtt tgacagatac gcactgtcct cgccgtcggc ggatgtgatg
     1321 gcaagatcgg taatctggcc gccctctacg gttacttcaa ctgcaatggt gccgccgaag
     1381 ccgtcagctt caccgtcata cgtaccgtcc acgtactgcc cttctgcttc tgctgcttca
     1441 cttgcggccg tatccgccgt gttggatgca gctgctgcgg cctgcagatt ttccttctga
     1501 agctgggtcg tctcaagctc tgccgagagc cgcgcgatct gatcctcttt gtctctttta
     1561 tcgagtacgg tattgtagcc gatcagaaca gcagcaatga cagcgacatt caaaaaacga
     1621 acaatcaatt gtctcatctg ttttcccctt tattgcgtta ctttcgcagc atccagcgca
     1681 ttccggcatg cctcaagaat ggcttttgag gagcaggttg cacgggaaac ggtgtcgatg
     1741 ttctcaagcg ttccggtttc cagaatctga gcggaaacgc ctttgatttt ggaggttcct
     1801 tcggatgcgc ggcggatgta ggaggcattc tgactgtctc cgtcaccggt gatgttgctt
     1861 aacgctacga ttttatcgtt ttcaattgtt accgtcacgg aaagctgata agcggtaaaa
     1921 tcttcatctt catccggctc gcagacggca atcgctgtgt attcgccgtc ctgatagatc
     1981 ttttgttccg gagtttccgt cggtgttggt gttggcgtct cagttggctc cggggtttcg
     2041 gtcggggttg gtgtttccgt cggtgtcgga gttaccgttg gcgtcggggt tggatttttc
     2101 ttttcctcag ttacgcgttc tgcctctttt aaagcttcct cgattgccgc aagaattccc
     2161 ctggagctgt aggttgcgcc ggaaacggtg tcgacttcca gtgtctggtt tttcacaacg
     2221 tcctttgctg tctgttttgc gcgttttaag aatgcttcgt catcttcact ctcggtcacg
     2281 agaattgcct tgatcgtctc atcctgaatg acaactgccg ttgtaatgtc tcccagatag
     2341 ccctcgccgg ttccgtagta aatgccttcc tcgtatggga attttccttc cggaatcgtt
     2401 gtggttccgt cgctgctcgt tccgttatcc ggaacggtct cgccgtttgt tgccttcttc
     2461 gcattttcca gcgcctgttt tactgcgttc tggatgccgc gggagctgta cgttgcgccg
     2521 gatacgagat ccacatcggt tttctgtccg tctacgatgc ggtccgctac gactttggca
     2581 cggttgaaga atgtctcgtc atcttcgttt tccagaatca gtaccgcttt gatcgtctga
     2641 tcctgcaggg ctaccgccac tttgatgtct ccctggaaac cggcggctgt gccgtaataa
     2701 attccgtcct gataagggaa acttcccttg gatgcagacg aattgttatt gttctgatta
     2761 ttattctggc tgttgtttcc gttgtttgta ttgctctggc ttcctccgtt tgtactggaa
     2821 gacccgttca ctgccgcctg acttaaggca ctccgcacag cttcaataat accgcgggag
     2881 ctgtatgtcg cgccggagac cgtatccaca ttcgtactct gtgttgagat aatcgtcgaa
     2941 atcagagagg aagcactgga aaggtagctt ggcgtatcgc tgttttccac aatatcaatc
     3001 gaagcaattt ttccgccgct gacaacaaca cagactttca ttgtgccgcc aaatcctgtt
     3061 ccggttccgt aatacgttcc atcccggtag gcagccggct cttcaatttt agataccgta
     3121 actgttccgc cggcagaggc tccgccgcct gccgctcctg atgcggatgc cggttctgaa
     3181 gtgtcctcct gaccggtcag tgcatttttc acggctgcaa ggataccttt tgaactgaaa
     3241 gttgcaccag aaaccgtgtc cacatccaca ctctgggatg cgatgatacg gtcgataact
     3301 cccttcgcac ggttcaggaa agctgcgtcc tcgccgggag cgcttacaat cgagatcgac
     3361 tgaatcgttt tgtctttcaa tacgacagag actgtaatcg taccgccaaa acctgttccg
     3421 cttccttcgt acgttccgtc cgccagctca aagctgccgg aaccgactgc tgcgacaagt
     3481 tctgcttcgg tatcggttgt ctttttggta tcgtccttct tttttgtatc atctttcttt
     3541 tccgctgcat cggtatcctg ttccggctct ggtgtcacca gtgcctccgg ctgctctacg
     3601 gcgtaaaccg gtgcagtgta gccgttcagc gaaataccgg cgcaggctgc tacgataact
     3661 gccgcgagag ccggtgcaaa tgctgtcagt tttttccatt ttcccatggt tatgtatcac
     3721 cgtctttcct gataatgttt gtctcgtgat cggatgcgaa atcatcggca attccttcgg
     3781 ttacatacag aactccgtcc tcatcctcca gaatggtgtc aaattcatcg ctgtgtgcac
     3841 gccagtat
//
LOCUS       DAOMUF010000187         3818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6750_length_3818_cov_2.992293, whole genome shotgun sequence.
ACCESSION   DAOMUF010000187 DAOMUF010000000
VERSION     DAOMUF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6750_length_3818_cov_2.992293"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLN77_09140"
     CDS             complement(<1..846)
                     /locus_tag="PLN77_09140"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRM44467.1"
                     /translation="MNTEPLTTLKGIGEKTEKLFAKVGIYNLQQLLHYYPRDYDCYAE
                     PVPLRECRQEEKNAVYGRIVHSPSVKGNQRKSVVVLELYEEPVRLQLTWFNMPFLRST
                     LKKGSVFIFRGKIQEKSGRLVMEQPEIFTPAAYHELLASLQPVYGLTAGLTNKMVSKA
                     VAQVLERCAPAQDYLPEKIRARYELCEINYALHEVHFPKDQEHLATARRRIVFEEFFL
                     FILSLRQLKEQTEGITNAFPIRAVWKTEEVIENMPYRLTNAQMRVWGELERDLKGHTR
                     MARLVQ"
     gene            complement(865..2544)
                     /locus_tag="PLN77_09145"
     CDS             complement(865..2544)
                     /locus_tag="PLN77_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRM44468.1"
                     /translation="MATNTIDAGLLAKMFLAGAKNLEVKKEWINELNVFPVPDGDTGT
                     NMTLTIMSAVKEVNSLTEVNMYNLSKAISSGSLRGARGNSGVILSQLLRGFTKKIRDY
                     EELNAEILAEAMEKAVETAYKAVMKPKEGTILTVAKGAAEKAAELAPESEDLNAFIED
                     VLAHAEYVLSQTPEMLPVLKEAGVVDSGGQGLLTVLQGAYDAFLGKEIDLNFEMPEAK
                     TEFVRPSKETEKEIKFGYCTEFIIMLENPLPDEEVYAFKDFLNGIGDSIVLVADDELV
                     KVHVHTNDPGKAISKALTYGQLTRMKIDNMREEHQERLIKNAEKIAAQQAEEDKKRKE
                     AAKQPPKENGFIAVSIGEGIKEIFQGLGVDYLIEGGQTMNPSTEDMLTAIEKVNAKNI
                     FILPNNKNIILAANQAKAMTEDKNIIVVPTKTVPQGITAMISYVPEKSAEENEEAMTE
                     GIQMVKTGQVTYAVRDTHIDEKEIHQGDIMGLGDHGLLAVGQDILTVAEETIRAMVDE
                     DSELISIYYGEDMSEEDAESLGEKIEQAYPDCDVEVNYGGQPIYYCVVSVE"
     gene            complement(2569..2925)
                     /locus_tag="PLN77_09150"
     CDS             complement(2569..2925)
                     /locus_tag="PLN77_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRM44469.1"
                     /translation="MKGRMNTGLGEIVINPDVIALYAGSVAVECFGIVGMAAVNMKDG
                     LVKLLKKESLTHGINVSISENKISLDFHVIVSYGVSISAVTDNLINNVKYKVEEFTGM
                     EIEKINILVEGVRVID"
     assembly_gap    3153..3162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3173..3358
                     /gene="rpmB"
                     /locus_tag="PLN77_09155"
     CDS             3173..3358
                     /gene="rpmB"
                     /locus_tag="PLN77_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRM44470.1"
                     /translation="MAKCAICEKGAHFGNNVSHSHRRSNKMWKSNVKSVKVKVDGVPQ
                     KMYVCTSCLRSGKVERA"
BASE COUNT          883 a          964 c          810 g         1151 t
ORIGIN      
        1 ctgtaccagc cgcgccatgc gggtatggcc ttttaagtcc cgctccagtt cgccccacac
       61 gcgcatctgt gcattcgtga gacggtacgg catattttcg atgacttcct ccgtcttcca
      121 gacggcgcgg atcgggaacg cattggtaat tccctccgtc tgctctttta actgccgcag
      181 ggacaggatg aagagaaaaa actcttcgaa cacaattctg cgccgcgccg ttgccagatg
      241 ctcctggtct tttggaaaat gaacctcatg gagggcataa ttgatttcac agagttcata
      301 ccgggcacgg attttctccg gcagatagtc ctgcgcaggt gcgcatcgtt ccagcacctg
      361 tgcgacggcc ttgctcacca tcttgttcgt gagaccggct gtcagtccat aaaccggctg
      421 aagacttgcc agaagctcat ggtatgcggc aggagtgaag atttccggct gctccatcac
      481 gagacggcct gatttttcct gtatctttcc gcggaagata aaaacactgc ctttcttcag
      541 cgtgctccgc agaaacggca tattaaacca ggtaagctgc agccgcaccg gctcctcgta
      601 gagttccagg acaacgacgc tctttctctg gtttcctttt accgacggac tgtggacaat
      661 tcttccgtat accgcattct tttcctcttg tctgcattcg cgaaggggaa ccggctctgc
      721 atagcagtca taatctcttg gataataatg cagaagctgc tgcaaattgt agattcctac
      781 ttttgcaaac agcttctccg ttttttcacc gatccccttc agtgttgtca ggggttccgt
      841 attcatattt ttctttcctt actcttattc aaccgaaacg acacagtagt aaatcggctg
      901 tccgccatag tttacttcca catcgcagtc cggatatgcc tgctcgattt tttcgccaag
      961 gctttcggca tcttcctcac tcatatcttc accgtagtag atgctgatca gctcgctgtc
     1021 ctcatcgacc atggcgcgga tggtttcttc tgcaacggta agaatatcct gtccgacagc
     1081 gagaagtccg tgatcgccaa gtcccatgat gtcgccctga tggatctctt tctcatcgat
     1141 gtgggtatca cgtaccgcgt acgttacctg accggttttc accatctgga tgccctctgt
     1201 catggcctcc tcgttctctt ctgcagattt ctccggcaca tagctgatca tggctgtgat
     1261 gccctgcgga acggttttgg tcggaacaac gatgatattt ttgtcctcag tcatggcttt
     1321 tgcctgattt gctgcgagga tgatattttt gttgttcggc agaataaaga tattctttgc
     1381 attgaccttc tcaattgcag tcagcatatc ctcggtgctc gggttcatgg tctgaccgcc
     1441 ctcgatcagg taatcaacac cgagtccctg gaagatctct ttgattccct ctccgatgga
     1501 cacggcgatg aatccgttct ccttcggcgg ctgttttgct gcttcttttc tcttcttatc
     1561 ttcctctgcc tgctgtgctg cgatcttttc tgcgttcttg atcaggcgct cctgatgctc
     1621 ctcacgcatg ttgtcgatct tcattctggt cagctgtccg tacgtcagcg ccttggaaat
     1681 tgcttttcca ggatcgttgg tatgtacgtg taccttaacc agctcgtcgt ctgcaacgag
     1741 aacgatcgaa tctccgattc cgttcaggaa atctttgaat gcataaacct cttcatccgg
     1801 aagcgggttt tccagcataa tgataaactc ggtgcagtag ccgaatttga tttctttttc
     1861 tgtctctttg gacggtctga caaattctgt ctttgcttcc ggcatctcga aattcagatc
     1921 aatctctttc ccgaggaacg cgtcatatgc gccctgcagt acggttaaga gaccctgtcc
     1981 gccggaatcc acaacgcctg cctcttttaa taccggcagc atctctggtg tctgggagag
     2041 cacgtactct gcatgcgcga gaacgtcctc gatgaatgcg ttcagatctt cgctttccgg
     2101 tgcaagctct gctgctttct ctgctgctcc ctttgccacg gtcaggatgg taccttcttt
     2161 tggcttcata actgctttgt atgcagtctc gactgccttt tccattgcct ctgcaagaat
     2221 ctctgcattc agctcttcat agtcgcggat cttcttggta aagccacgaa ggagctggga
     2281 aagaataacg ccggagttac ctctggcacc gcgcagagaa ccggaagaga ttgccttgga
     2341 aaggttgtac atgttcactt ctgtgagcga gtttacttcc ttgactgcgg acatgatcgt
     2401 cagcgtcatg ttggtacctg tgtcgccgtc cggtaccggg aacacgttca gctcattgat
     2461 ccactctttc tttacttcca gattttttgc accagccaga aacatttttg ccagtaagcc
     2521 ggcatcaatt gtatttgtag ccactttctt catttcctcc tattaccatc agtcgatgac
     2581 gcgaacgcct tcaaccagga tgttgatttt ttcaatttcc attccggtga actcttccac
     2641 cttgtatttc acattattga tcaggttatc tgttacggca gaaatactga ctccgtaaga
     2701 aacaatcacg tggaaatcca gggaaatttt attttccgaa atcgatacat tgattccgtg
     2761 tgtcagactc tcttttttca gaagtttgac cagtccatct ttcatgttta ccgctgccat
     2821 tccgacaatg ccgaagcact ccacagcaac gctgcccgca tacagggcga ttacatccgg
     2881 attgattacg atttctccca agcctgtatt catacgacct ttcatgcctt gtatcctcca
     2941 attccatcat actattcaaa acacatggag acaggaataa ctgactccac tctacgatac
     3001 attataaccg cttttccgca aaaaagaaag tttaaaatga aaaaccttca aaattcatat
     3061 aattttttca ttttttactt gcaaaaccgg tatagatctg ttagaatgac ttatgttgtg
     3121 agtctaaaat gcgttttgac tcaagtacgt atnnnnnnnn nnaggtgcaa tcatggctaa
     3181 atgtgctatt tgtgaaaagg gtgctcattt cggaaacaac gtaagtcatt cacatagaag
     3241 aagcaataaa atgtggaaat ctaacgtaaa atctgtcaaa gttaaagttg acggtgtacc
     3301 gcagaaaatg tacgtatgta cttcctgctt aagatccgga aaagtagagc gtgcataagt
     3361 tgaatggtta aaaggccttt ccagtccggg aaggcttttt tgctgttcaa aaatagaatt
     3421 aagattttaa aaaaagttct gggaagagca aacaggacat aagttttcgc ttatgccctg
     3481 ttgtgctcta ttttttatat tttaaaaggg agaaaaaaat agcgtggtct cttaagctga
     3541 acctgccaac gatccatatg cttagcaaaa gaaaaagagc atcctaccga aactttcgtt
     3601 ctggtaagac gccctcgtct tgatctatga tattagtata gttggctgat tcatttaaga
     3661 tggacttatt atagcgcagc tttcttcatt ttgcaagtat ttctctgtac aaatcagcca
     3721 gttcatttcc gatctttttg tgcatctttc actattcccc tgtattgcag aacagatgat
     3781 atcccacgca cagcagcaga gcggccgcaa gaaccgta
//
LOCUS       DAOMUF010000188         3779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6843_length_3779_cov_2.818206, whole genome shotgun sequence.
ACCESSION   DAOMUF010000188 DAOMUF010000000
VERSION     DAOMUF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6843_length_3779_cov_2.818206"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PLN77_09160"
     CDS             <1..831
                     /locus_tag="PLN77_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRM44471.1"
                     /translation="ELNSVMTVQGVPTDEITLEVKKADGKKKTITYEPTVTTRYMMGF
                     NYDDCDRGMEFTYVQQNMPLAAAGVVAGDLLVSINGAPITCVADFTAYQTEHPFDGSA
                     VTLEYEHSGKTKEITVTPVENTYANVQFSYQLREKQSPIGVLKYSVLEIKYWVRTVID
                     SVSMLITGQYSVNDLSGPVGVVDAIGTAYDDVKSQGVLVTVMTMLNMVILLSSNLGVM
                     NLLPLPALDGGRLVFLIIEAIRRKPIRRDLEGMVHFAGLVLLMALMVYVMIHDVQRIL
                     "
     gene            905..>1940
                     /locus_tag="PLN77_09165"
     CDS             905..>1940
                     /locus_tag="PLN77_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRM44472.1"
                     /translation="MKNLRMQQEMTDRGRLEIRVNARIQARPVSGARVTVSYTGDPQN
                     VIEELITDGDGRTETLDLATPPLEYSLEPNVNQPYSEYTVKVEAEGYEPVEVTGSELL
                     SGEQSVQQVDLEPAEGAAFADVTIPDHTLFGEYPAKIPESEIKPTGESGEIVLSRVVI
                     PEYVVVHDGAPTDSTARDYYVRYRDYIKNVACSEIYATWPDAAIRANILAIMSFTLNR
                     VYTEWYRNKGYDFTITSSTAYDHKWIYGRNIFDSISLVVDEIFADYLSRPNVKQPILT
                     QYCDGNRVSCPNWMSQWGSKNLADQGYSTIQILRNYYGDNMYINTAEEISGIPSSWPG
                     YDLTIGSSGNK"
     assembly_gap    1941..1950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1951..2166
                     /locus_tag="PLN77_09170"
     CDS             <1951..2166
                     /locus_tag="PLN77_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRM44473.1"
                     /translation="LQMQEQLNVIAEVYSSIPTVYENGYFDEETQDAVEAFQRLFGLP
                     VSGIVDYPTWYKIQSIYVAVTRIAELQ"
     gene            2306..2671
                     /locus_tag="PLN77_09175"
     CDS             2306..2671
                     /locus_tag="PLN77_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HRM44474.1"
                     /translation="MITKVIAMEKILEQTLLYDFYGELLTEHQRQVYEDVVLNDFSLS
                     EVAAARGISRQGVHDLVRRCNKTLEEYEEKLHLVQRFVQIRENVNEIRKLTDPSGDTP
                     KEDVMQRIAAIASDILEEL"
     gene            2674..>3779
                     /gene="ffh"
                     /locus_tag="PLN77_09180"
     CDS             2674..>3779
                     /gene="ffh"
                     /locus_tag="PLN77_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRM44475.1"
                     /translation="MAFESLTEKLQNVFKNLRSKGRLTEADVKTALKEVKMALLEADV
                     SFKVVKQFIKSVQEQAVGQDVMNGLNPGQMVIKIVNDELVKLMGSETTEIALRPASEV
                     TIIMMAGLQGAGKTTTTAKIAGKLKAKGRKPLLAACDVYRPAAIKQLQVNGEKQGVEV
                     FAMGDKNRPVDIAKAALVHAKTNGFNVLILDTAGRLHIDEEMMQELQDIKAAVPVDQT
                     LLVVDAMTGQDAVNVSEMFNNKVGIDGVVLTKLDGDTRGGAALSIRAVSGKPILYVGM
                     GEKLSDLEQFYPDRMASRILGMGDVMSLIEKAQANIDEEKAKAMEQKMKKAQFDFNDY
                     LDSMEQMRNMGGISSILNMLPGVGAKMKGQDLES"
BASE COUNT         1009 a          852 c         1098 g          810 t
ORIGIN      
        1 gagctgaact ctgtgatgac ggtacagggc gtgccgacgg atgagattac gctggaggtt
       61 aaaaaggccg acgggaaaaa gaaaacgatc acatatgaac cgacggtgac aacgcggtat
      121 atgatgggct tcaattacga tgactgtgac cgtggcatgg aattcacgta tgtgcagcag
      181 aatatgccgc ttgccgctgc gggtgttgtc gcgggcgacc tgctcgtatc gatcaacgga
      241 gcgccgatca cctgtgtggc agattttact gcttaccaga cagagcatcc tttcgatgga
      301 agcgctgtga cgctggagta tgagcacagc ggaaagacga aagagattac ggtgacaccg
      361 gttgaaaaca cgtatgcgaa tgtacagttt tcgtatcagc tccgggagaa acagtccccg
      421 atcggcgtgc tgaaatacag cgtgctggag atcaaatact gggtgcgcac cgtcattgac
      481 agcgtcagta tgctgatcac cggacagtat tctgtgaacg atctttccgg cccggtcggc
      541 gtggtggatg cgatcggaac cgcctatgat gatgtaaaat cgcagggcgt tctggttacg
      601 gtgatgacga tgctgaacat ggtgattctg ctgtcatcga accttggcgt catgaacctg
      661 ctgccgctcc cggcactcga cggcggacgg cttgtctttc tgatcataga agccatccgc
      721 agaaaaccaa tccggcgaga cctggagggt atggttcact tcgcgggcct ggtgcttctg
      781 atggcgttga tggtctacgt catgatccac gacgtccagc ggatcctgta aatcgaaaag
      841 tgaaaagcaa caaagcctca tattctgcat accataaaag gagtgcagga tatggggctt
      901 ttttatgaaa aatttgcgta tgcagcagga aatgactgac cgcgggcggc tggaaatccg
      961 ggtaaatgca agaatccagg cgcggcctgt ttccggggcg cgggtgacgg tttcgtatac
     1021 gggagatccg cagaatgtga tcgaggagct gattaccgac ggggacggca gaacggagac
     1081 gctggatctg gcgacgccgc cgctcgagta cagtctggaa ccgaatgtga atcagccgta
     1141 ttccgaatat acggtgaaag tggaggcgga agggtatgaa ccggttgaag tgacgggctc
     1201 cgagcttctt tcaggagaac agtccgtcca gcaggtggac ctcgaaccgg cagagggcgc
     1261 agcgtttgcg gatgtgacga tcccggacca tacgttgttc ggcgaatatc cggcgaaaat
     1321 tccggaaagt gagatcaagc cgacggggga gagcggtgag attgtgctgt cccgcgtcgt
     1381 gatcccggag tatgtcgttg tccacgacgg agcgccgacc gacagcacgg cgcgcgacta
     1441 ttatgtgcgg taccgcgact acatcaaaaa cgtggcgtgc tcggaaatct atgcaacatg
     1501 gccggatgcg gcgatccggg cgaatatcct cgcaatcatg tcatttacgc tgaaccgtgt
     1561 ctatacagaa tggtaccgca acaagggata cgattttacg atcacttcgt cgacagccta
     1621 cgatcacaaa tggatttacg gcaggaatat tttcgacagc atttcccttg tggtcgatga
     1681 gatttttgcc gattacctgt cgcgcccgaa tgtgaaacaa ccgattctga cgcagtactg
     1741 tgacggaaac cgtgtgagct gcccgaactg gatgagccag tgggggtcca aaaatctggc
     1801 ggaccaaggg tattcaacga ttcagattct ccgcaattat tatggagaca atatgtatat
     1861 caacacggcg gaggagattt ccggcatccc gtcctcctgg ccggggtatg acctcaccat
     1921 cggctcgtcc gggaacaagg nnnnnnnnnn ctgcagatgc aggagcagct gaacgtgatt
     1981 gcggaggtgt attcatcgat tccgacggtc tatgaaaatg gctatttcga tgaagagacg
     2041 caggacgccg tcgaagcgtt ccagcggctg ttcggcctgc cggtttccgg cattgtggat
     2101 tatccgacgt ggtacaaaat ccagtcgatt tatgtggctg tgacgaggat tgcggagctg
     2161 cagtaggtat acaaaagtct ttataagggg cgtgttttct gcgaaaaaac gcggaaaatg
     2221 cgccttttct gcggcttttg aaacggatca gaaaaatttt aaaactgtca agaaaaaata
     2281 cttgacaccc agcatcattt cgtgtatgat aaccaaggtg atcgcaatgg aaaaaatatt
     2341 ggaacagaca ttactgtatg atttttacgg cgaacttctg actgaacatc agcgtcaggt
     2401 atacgaggac gtggtgctca acgatttttc cctgagcgag gttgcggcag cgcggggcat
     2461 ttcccgccag ggagtccacg atctggtcag acgctgcaac aagacactgg aagagtatga
     2521 agaaaagctt catctggtac aacgctttgt acagatcagg gaaaatgtga acgagatccg
     2581 aaagctgaca gacccatccg gtgatacccc gaaagaagat gtgatgcagc ggattgcggc
     2641 aattgcatcg gatatattag aggagctttg agcatggcat ttgaaagttt aaccgagaaa
     2701 ctccagaatg ttttcaaaaa tctcagaagc aagggccgtc ttactgaggc ggatgtaaag
     2761 actgcgctga aggaagttaa aatggcactt ctcgaggcgg atgtcagctt caaagtagta
     2821 aaacagttta tcaaatccgt acaggagcag gcagtcggac aggacgtcat gaacggactg
     2881 aacccgggac agatggtcat taaaatcgta aatgacgagc tggttaagct gatgggctcc
     2941 gagacaacgg agattgcact gcgcccggca agcgaagtta ccatcatcat gatggcaggt
     3001 cttcagggag ccggtaaaac aaccacaacc gcaaaaattg caggaaagtt aaaagcaaag
     3061 ggaagaaaac ctctccttgc cgcctgtgac gtctatcgcc cggcagcgat caaacagctg
     3121 caggtcaatg gagaaaagca gggcgtggaa gtgttcgcca tgggagataa aaaccgtccg
     3181 gtggatatcg caaaggcagc gctagtgcat gcaaagacaa acggcttcaa tgtattgatt
     3241 ctggatacgg caggccgtct gcacatcgac gaagagatga tgcaggagtt acaggatatc
     3301 aaagcggctg ttcctgtgga tcagaccctt ttggtagtag atgctatgac cggacaggac
     3361 gcagtcaatg tgtccgagat gttcaacaat aaagtcggca tcgacggcgt agtacttaca
     3421 aaattagatg gtgacaccag aggcggtgcg gcactttcga tccgcgcagt ttccggcaaa
     3481 ccgattctct atgtcggcat gggcgaaaag ctttcggatc tggagcagtt ctatccggac
     3541 cgtatggcgt cccgtatcct cggcatgggc gatgtgatga gcctgattga aaaagcacag
     3601 gcaaacatcg acgaagagaa agcaaaagcc atggaacaga agatgaagaa ggcgcagttc
     3661 gacttcaacg attatctgga ttccatggag cagatgcgca atatgggcgg catctccagc
     3721 atcttgaata tgcttccggg cgtcggcgcg aaaatgaagg gccaggatct tgagagcat
//
LOCUS       DAOMUF010000189         3763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6885_length_3763_cov_4.270496, whole genome shotgun sequence.
ACCESSION   DAOMUF010000189 DAOMUF010000000
VERSION     DAOMUF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6885_length_3763_cov_4.270496"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLN77_09185"
     CDS             <1..307
                     /locus_tag="PLN77_09185"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRM44476.1"
                     /translation="GYLITSIPYDENFVLKVDGKETMLFRANTAFLGAKIPSGEHKIV
                     LVYHAPGRTAGSWCSLMGGMLALILVICEKKKQQNGSERAGESKMLQKQRKYYIIRV"
     gene            643..1035
                     /locus_tag="PLN77_09190"
     CDS             643..1035
                     /locus_tag="PLN77_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM44477.1"
                     /translation="MELREQLNGIQHVGIPTNDIEMTIAFYENLGFEIAFRTVNEAAD
                     EKVAFLKLGTLVIETYENKAAALKPGAIDHVAIDVKNIEKVHEMITGAGLNTTQDEVH
                     FLPFWENGVRFFTIEGPNKEKVEFSQYL"
     gene            1394..1672
                     /locus_tag="PLN77_09195"
     CDS             1394..1672
                     /locus_tag="PLN77_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM44478.1"
                     /translation="MEQFIWETPEELDQKLAQRVRKIRKRRSITQEKLASISGVSYGS
                     IKRFETTGQISLLSLTKIAIALDLADELRNIFTQVPYKDIQEVINETR"
     gene            1659..2798
                     /locus_tag="PLN77_09200"
     CDS             1659..2798
                     /locus_tag="PLN77_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HRM44479.1"
                     /translation="MKQDKVLQVCYEDQTVGTLAMTADHKVAFQYSDEWLETGFSINP
                     FSLPLQKQVFVPVKDHFEGLFGVFADSLPDHWGRLLLDRLLRAHKQNPDELTVLDRLA
                     IVGTSGMGALTYHPEKNFPEEQSSADLDKLAEECQKILNTEYSDKLDELYLLGGTSGG
                     ARPKIMTTIDNEEWIIKFPAHVDGKDAGKMEYDYSCCAKACGIMMSETRLFPSEKCKG
                     YFGTKRFDRRNCLKGKKKVHMLTTAAILELDFEQPSLDYHGLMKLTKIMTRNCDEDVE
                     NMFRRMCFNVFAHNRDDHSKNFTYLYEADEDKWHLSPAYDLTYSNTYYGEHTTSVDGN
                     GRNPGRKELLAVGTGAGMKKTRCEEIIDEIEKKVKEMLEEYLLGK"
     gene            complement(3008..>3763)
                     /locus_tag="PLN77_09205"
     CDS             complement(3008..>3763)
                     /locus_tag="PLN77_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRM44480.1"
                     /translation="YESSFSLQIWKVYGDEISIELISPGGIRSGTLSPVLGTARLTLG
                     PTELLLFYGMPSPYSQAQEIYLTFQGAGNHLSVENGIWTLRLTPGRLISGSFDLWLPG
                     GNAVGSQTRFFSPTADTTLTIPSTARSAITVGAYDPRLSSYASFSGRGYTRILHEIKP
                     DLAAPGVNIPAPRSGGGYASFTGTSFATPFVSGSAALMMEWGILRGNDPFLYGEKLKA
                     YLIAGAKPIASESEYPNRRVGWGRLCLESSLPD"
BASE COUNT         1187 a          709 c          977 g          890 t
ORIGIN      
        1 cggatacctc atcaccagca ttccatacga tgaaaatttt gtgctgaaag tagatgggaa
       61 agaaacaatg ctgttccgcg caaacacggc atttctcggc gcaaaaatcc catcaggcga
      121 gcacaaaatt gttcttgtgt accatgcgcc cggacggacg gctggcagtt ggtgcagcct
      181 gatgggagga atgctggcac tcatccttgt gatctgtgaa aaaaagaaac agcaaaacgg
      241 cagcgaacgg gcaggagaga gcaaaatgtt gcaaaaacag cggaaatatt atatcatcag
      301 agtgtaaaaa tagttgtagt gacacttggt ggaatggagc atatctttac agccgcagga
      361 agaactggca gcgtttgccc gttttggaaa cgccgttgca agcctttgtg tggagaaaaa
      421 aggtgctaga gcgtgtctga aaaatcattt ccgcaatctg caagccccac tttgcggtat
      481 attttaccct cattcggttg ccgtagcccg ctacgccgcc cttattcgga taaaatctcc
      541 cacgaactgg gactcgcagt tcacgaaaag cctttttcag acacgctcta ttccggccat
      601 gccggatctg gcacaggtag aaaaaagact gggggataag aaatggaatt aagagagcag
      661 ttaaatggaa tccagcatgt cggtattccg accaacgata ttgagatgac gattgcgttt
      721 tatgaaaatc ttggatttga aattgcattc cgcaccgtaa atgaggcggc ggatgagaaa
      781 gtggcattct taaagcttgg aacacttgtg atcgaaacgt acgaaaacaa agctgcagca
      841 ctgaaacccg gagcaatcga ccatgtggcg attgacgtaa agaatattga aaaggttcac
      901 gaaatgatta ccggggccgg tctgaatacg acgcaggatg aagtacattt ccttccgttc
      961 tgggaaaatg gcgtccgttt cttcacgatc gaaggaccaa ataaagaaaa agtggaattc
     1021 agccagtatt tgtaacgatt ccaagccatg cgaaatggaa gaaaaatcgc gaatcatgct
     1081 tgcatgaata tccgtttgac cagcaaagcc tctgccggga agcaaaattt ttgcccggca
     1141 gaggcttttt tacttactct gaaacaaata ctaccggata gtcaaaaaag cgcaaattat
     1201 ccctatagag taaaggagat gcaaaattta tctgcagtta caaaaacggg gtggagtttt
     1261 tatggaaaca gggcaggaaa tcattgtgct ttctgcacgt gagtggtaaa atagaataga
     1321 taatatataa tctgttttga ataaaaacag gatattttag ataacatata atctgtaaca
     1381 agaaggggaa aaaatggaac aatttatatg ggaaactccg gaagaactgg atcaaaaact
     1441 tgcgcagcga gtgagaaaaa tcagaaagag acgttcgatt acgcaggaaa aattagcatc
     1501 tataagcgga gtgagttacg gctcaatcaa aagattcgaa acgacaggac aaatttccct
     1561 gttatcttta acgaaaattg caatcgcact ggatcttgca gatgagctca gaaatatttt
     1621 cacgcaggta ccgtataaag atattcagga ggttatcaat gaaacaagat aaagtactgc
     1681 aggtgtgtta tgaggatcaa acggtgggaa cattagctat gacggcagac cacaaagtgg
     1741 catttcagta cagtgatgaa tggctggaaa ccggattttc gatcaaccca ttttcattgc
     1801 ctttgcaaaa acaggtattt gttccagtta aggatcattt tgaaggatta tttggggttt
     1861 ttgcggacag tcttccagat cattggggca gattactgtt ggatcgcctg ttacgggcac
     1921 ataaacaaaa tccggatgaa ctgactgtac tggatcgtct ggcgattgtg ggcacatcgg
     1981 ggatgggagc gttgacatat cacccggaaa agaattttcc agaggagcag agcagtgcag
     2041 atctggacaa actggcggaa gagtgtcaaa agatactgaa tacagagtat tcggataaac
     2101 tggacgaatt gtatctgctg ggaggtacca gcggcggagc aagacctaaa atcatgacaa
     2161 ccattgataa tgaagaatgg attatcaaat ttccagcgca tgtggacgga aaagatgcag
     2221 gaaaaatgga atatgactat tcctgctgtg ccaaggcctg cggaattatg atgagtgaaa
     2281 cgagattgtt tccatcagaa aaatgtaaag gatattttgg aactaaaagg tttgacagga
     2341 gaaactgttt gaaagggaaa aagaaagtgc acatgttgac gacagctgca attctggaat
     2401 tggattttga gcagccaagt ctggactatc atggattaat gaaactgaca aaaatcatga
     2461 cacgcaattg cgatgaagat gttgaaaata tgttccgccg aatgtgtttc aatgtttttg
     2521 cacataacag agatgatcat tcgaagaact tcacgtattt gtacgaagca gacgaagata
     2581 aatggcattt aagtccggca tatgatctta cttacagtaa tacgtattat ggcgaacata
     2641 ctacgtcagt agatggaaat ggaagaaatc caggcagaaa agagttactg gcggtaggaa
     2701 ctggtgcagg gatgaaaaag acaaggtgtg aagagatcat agacgaaatt gagaaaaaag
     2761 taaaggaaat gttggaagaa tatcttttgg gtaagtgatt ctctttgcat tcaaaaaatt
     2821 ttaaacagca agttattaac agttaaataa aaaagagttg ccagacaaat gaacactata
     2881 agtaccttgc tacgagtttt attttcgtag caaagtacaa gatagtacag agataaaaat
     2941 atgtctcatt tgtcgtttaa ctctttacat tgcgctttta tttcttctat cgaatccttc
     3001 aataacttta atctggcagg ctactttcta aacaaagtcg tccccatccc acccgcctgt
     3061 tcgggtattc gctctcggat gcgatgggtt ttgcacctgc aatgagataa gctttcagtt
     3121 tttcaccata taaaaacggg tcatttccgc gcagaatacc ccattccatc ataagcgcgg
     3181 cgcttcccga tacaaaaggc gtggcaaacg aggtgccggt gaaggaagca tagccgccgc
     3241 cggaacgggg agctgggata ttgacacccg gggcggcgag atcaggttta atttcgtgga
     3301 gaatacgggt atagccgcgg ccggagaagc tggcatagga ggaaaggcgg ggatcgtagg
     3361 caccgaccgt gatggcggag cgggcggtgg aagggatggt gagtgtggtg tcggcagtgg
     3421 gggagaagaa gcgtgtctgt gaaccgacgg cgtttccgcc gggaagccag aggtcaaagg
     3481 aaccggagat caggcggccg ggggtgagac gcaaggtcca gatgccgttt tcgacggaca
     3541 gatggtttcc tgcgccctga aaggtcaggt aaatttcctg cgcctggctg tagggggatg
     3601 gcatgccata aaacaggaga agttctgtcg gaccaagcgt cagacgcgcc gtgccgagga
     3661 caggagagag cgtgccggag cggatgccgc cgggggaaat cagttcgatc gagatttcat
     3721 ccccataaac cttccagatc tggagactga acgaggattc ata
//
LOCUS       DAOMUF010000190         3739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6942_length_3739_cov_3.722041, whole genome shotgun sequence.
ACCESSION   DAOMUF010000190 DAOMUF010000000
VERSION     DAOMUF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6942_length_3739_cov_3.722041"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..241)
                     /locus_tag="PLN77_09210"
                     /pseudo
     CDS             complement(119..241)
                     /locus_tag="PLN77_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512622.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(306..2381)
                     /locus_tag="PLN77_09215"
     CDS             complement(306..2381)
                     /locus_tag="PLN77_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRM44481.1"
                     /translation="MVKVGKWIAKHRILMLIIGFLLIIPSVIGMAATKVNYDLLSYLP
                     EHLETVKGQDILVDEYGMGAFSMVVVENMEMKDVQKLEEKFEQVNHVKEVLWYDDVAD
                     ISVPVEMIPEKLRKAFYNGDATMMLVLFDNTTSSDDSMEAIEDLRKIANEQCFIGGMT
                     GVVTDIKNVALKELPVYVVIAAVLSLVVIELTSTSFVVPILFLLSIGLAILYNLGSNV
                     FLGETSYITKALTAVLQLGVTMDYSIFLLNSFEENKKRFPDDKERAMGHAIANTFKSV
                     AGSSVTTVAGFLALCVMTFALGRDLGIVMAKGVLIGVVCCVTVLPAMVLVFDKAIEKT
                     RHKPLVKSLDKPSAFITKHYKVWVVIFLVLLFPSIYGNNHTQIYYNIARSLPSTLDCN
                     VANDEVKAQFNVSNMHIVMMDRDMDSKQKKKMMEEIGNVKGVKSTISMSSLLGPTIPE
                     SMIPENLRSMLQSDEYELAFVSSEYESATDEVNEQVKAIDKIVKSYDKDGMVIGEAPL
                     MKDLQDVTDADLVNVNVLSIGAIFIIILLIFKSISLPIILVAVIEFAIMLNMAIPYYQ
                     GTSLPFVASIVIGAIQLGATVDYAILMTTRYQKERQNGKDKKEAISIAHKTSMPSIIS
                     SGLSFFAATFGVACYSQVEMIGSICTLLARGAIISMVVVILILPAMFMIFDKLICKTS
                     IGFLKKKTK"
     gene            complement(2610..3488)
                     /locus_tag="PLN77_09220"
     CDS             complement(2610..3488)
                     /locus_tag="PLN77_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRM44482.1"
                     /translation="MKIAVTYDNGNIFQHFGKTENFKVYEVEDNQVVSSEVIGSNGTG
                     HGALAGLLSEQGVDVLICGGIGGGAQAALAEAGVELCSGAEGDVDAAVEAYLNGTLTS
                     AGATCDHHDHEEGHSCGGHCGSHEEEESSCGGSCGTCGGGCGAPTPVMEGKNVGKTCR
                     THYRGTFNDGTQFDSSYDRGEPLEFICGVGQMIRGFDAAVAEMEVGEKVNIHLMPEEA
                     YGMPDPNAIFTVAIAQLPGAEEVEVGQQVYLQNQYGQPFPVKVTAKDETNITFDANHE
                     MAGKELNFEIELVEVK"
     gene            complement(3516..>3739)
                     /locus_tag="PLN77_09225"
     CDS             complement(3516..>3739)
                     /locus_tag="PLN77_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRM44483.1"
                     /translation="LGIDVHDVTAAWNDKLRPGAIITDEPGLYIDEWEIGIRIEDDIL
                     ITEDGCEVLSENLMRDPDQIEAFMQEKEA"
BASE COUNT          894 a          969 c          820 g         1056 t
ORIGIN      
        1 caatgtctgc tcactgtttc tacgtattct tgccattttt tcttctcctt tagtccttgt
       61 ccgtcctatc ttgcagtgtc tttccgattt cttttgcctg attccgtaca cttcggatca
      121 cgccattatc ccttacagaa taatggattg catcgatgta gagaatagga tagacctcgt
      181 ccagtggccg gttctgccag tcttcaatcc gtggcagaat cttatccgtc acatcagaaa
      241 tttgaacata aagcaaaaaa attcccccgc accagctact tccggtacag gggatttgtc
      301 atactttatt ttgttttctt ctttaaaaat ccaatcgatg tcttacagat cagtttatcg
      361 aaaatcataa acattgccgg cagaatcagg ataacaacga ccatactgat gatcgcgccc
      421 cgtgcaagca gagtacagat ggaaccgatc atctcaacct gagagtagca ggccacaccg
      481 aaggttgcgg caaagaagct cagaccactg ctgatgatgg acggcatact ggtcttatgg
      541 gcaatactga tagcttcctt cttgtctttt ccattctggc gctctttctg ataacgggtt
      601 gtcatcagga tcgcgtagtc cacggttgca ccgagctgaa tcgctccgat tacgatgctg
      661 gccacgaatg gaaggctggt tccctgataa tacgggattg ccatattcag cataattgca
      721 aactcgatga ctgctaccag aataatcggc agagaaatcg atttgaagat cagcagaatg
      781 atgataaaga ttgcaccaat ggaaagcacg tttacgttga caaggtcagc atcggtaaca
      841 tcctgcaggt ctttcatcaa cggtgcttca ccgatgacca ttccatcttt atcgtaggat
      901 tttacgattt tgtcaatcgc tttgacctgt tcgtttactt catctgttgc ggactcatat
      961 tcagagctca caaatgcaag ctcatactca tcgctctgca gcatgcttcg gagattttcc
     1021 gggatcatgg attccggaat cgtcggtcca agcagggaac tcatgctgat ggtcgatttg
     1081 acacctttta cgttgccgat ctcctccatc attttctttt tctgtttgga atccatatcg
     1141 cggtccatca tgacgatgtg catgttgctg acgttgaact gcgcttttac ttcatcgttt
     1201 gccacgttac agtccagggt agatggaagg gaacgtgcga tgttgtaata aatctgggta
     1261 tgattgtttc cgtagatcga cgggaacagc agaaccagga agatcacaac ccatacttta
     1321 taatgtttgg tgataaaggc ggacggttta tccaggcttt ttaccagcgg tttgtgtctc
     1381 gttttctcaa ttgccttgtc aaataccaga accatggccg gcaggacggt gacacagcag
     1441 acaacgccga tcaaaacacc ttttgccata acgataccaa ggtcgcggcc aagggcaaag
     1501 gtcataacac acagtgccag gaaaccggct acggtggtaa cggagcttcc ggctacggat
     1561 ttgaacgtgt tggcgattgc atggcccatc gcacgctctt tgtcatccgg gaaacgcttt
     1621 ttattctcct cgaagctgtt cagcaggaag atggagtagt ccatcgtaac accaagctgc
     1681 agcacggcgg tcagcgcttt tgtgatgtag gaagtctcac caaggaacac gttgcttccc
     1741 aggttgtaaa ggattgcaag tccgatactt aacaggaaca ggatcggaac aacgaaggaa
     1801 gtcgatgtca gctcgatgac aacgaggctc aaaactgcgg cgatgacaac gtaaaccggc
     1861 agctctttta atgctacgtt tttgatatcg gtaacaacac cggtcatacc gccgatgaag
     1921 cactgctcgt tcgcaatttt acgaagatcc tcgattgcct ccatggagtc atcggaagaa
     1981 gtcgtattgt cgaacagaac gagcatcatg gttgcatccc cattgtagaa tgctttccgg
     2041 agtttttccg ggatcatctc aaccggcacg ctgatgtctg ccacatcgtc ataccataaa
     2101 acttctttta cgtggtttac ctgctcgaat ttttcttcga gtttctgtac gtccttcatt
     2161 tccatgtttt ccacgacaac catggagaac gcacccatgc cgtattcatc taccagaatg
     2221 tcctgtcctt ttacagtttc cagatgctcc ggcagatagc tgagcagatc atagtttact
     2281 ttcgttgctg ccattccgat gactgacgga ataatcagca gaaatccaat gatcagcatc
     2341 agaatcctgt gcttggcaat ccatttgcca actttaacca ttttcattca tctcctctga
     2401 ttcattttta acttgttttt ctagtaccac atcttacaac ttaaatgact attagtcaat
     2461 atttgaaatg gcaaaaaatg aattttggtc attttttgtt tacttttaca aaaaataacg
     2521 gaaatcggaa atgggaatcg ttgatttttc tgcgcatcaa aaaagagtgc ctggctcctt
     2581 cgtcctgaca ctctttttta tgcatttttt tatttcactt caaccagctc aatctcaaaa
     2641 ttcagctctt ttccagccat ctcatggttg gcatcaaacg tgatattggt ctcgtctttt
     2701 gcggtaactt ttaccgggaa cggctggccg tactggttct gcagataaac ctgctgtccg
     2761 acctctactt cctctgctcc cggaagctgt gcgatcgcaa cggtgaaaat tgcgttcgga
     2821 tccggcattc cgtaagcctc ctccggcatc agatggatgt tgactttttc gccgacttcc
     2881 atctctgcaa cggctgcatc gaagccgcgg atcatctggc ccacaccgca gatgaactcg
     2941 agcggttcgc cgcggtcgta ggaagaatca aactgggttc catcgttgaa ggttcctctg
     3001 tagtgggtac ggcaggtttt tccgacattc ttaccttcca taaccggagt cggtgcaccg
     3061 catccgccgc cgcaggttcc gcaggaaccg ccgcaggagc tctcctcttc ctcatggctt
     3121 ccgcagtggc cgccgcagga atggccttcc tcgtgatcgt ggtgatcgca ggttgcaccc
     3181 gcagatgtga gcgttccgtt taagtaagct tccaccgctg catccacatc tccctccgct
     3241 ccggagcaga gctctacgcc tgcctcagca agagcagcct gtgcaccgcc gccgatgccg
     3301 ccgcagatca gaacatccac gccctgctcg gaaagaaggc ctgccagtgc gccgtggccg
     3361 gttccgttgg atccgatcac ttcgctggaa accacctgat tgtcttctac ttcgtaaacc
     3421 ttaaaattct ctgtttttcc aaaatgctgg aaaatattcc cgttatcgta tgtaactgca
     3481 attttcatct aactaaatcc tccgtagttt ttttcttacg cctctttttc ctgcataaag
     3541 gcttcaatct ggtctggatc acgcatcaga ttctcggaaa gaacctcgca tccgtcctct
     3601 gtaatcagga tatcatcctc gatccggatt ccgatctccc actcgtcaat ataaagaccc
     3661 ggctcatcgg tgatgatggc gcctggacgc agcttgtcgt tccatgccgc ggtcacatcg
     3721 tgtacatcga tgccgaggt
//
LOCUS       DAOMUF010000191         3736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6951_length_3736_cov_3.019832, whole genome shotgun sequence.
ACCESSION   DAOMUF010000191 DAOMUF010000000
VERSION     DAOMUF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6951_length_3736_cov_3.019832"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="PLN77_09230"
     CDS             <1..1843
                     /locus_tag="PLN77_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM44484.1"
                     /translation="YFSTQEIVTVLNYLHLCDNPCQEIPFTAILRSPICGCTDTELAA
                     VRCVDKDVKIYEACGKYAAMGDDEALREKLRRFLAQLETLRSRVPYTPIHELITQILE
                     ETGYGGYASAMPGGVQRRANLEMLVEKAVEFEATSYRGLFNFIRYIEQLQKYEVDFGE
                     VNIYGESADTVRIMSIHKSKGLEFPVVFLSGMGKSFNQMDSRAALVLHSRMGIGADAI
                     DPEHRVRQATLPRQIIRQSMKLENLAEELRVLYVAMTRPKEKLIMTGMASKLESRVKQ
                     CAAHAGRQEELLSYQTLSSASGYLDWILPAFSRNRCFDGLYEQFGLAGNADNPLYAEK
                     IPVTIRLVSPAGLVEGEVLHQMERAVNEQKIMEIPKQPPREAALKELVEERFAYQYPY
                     EKEKQIPVKVTVSQLKKAGMEESEIGKELFAQEELVPIVPGFLKKDHVAALTGSDRGT
                     AYHRLLECLDYRRAYSITELMAQTEELIAEGKMTKEAAECIYFKSILAFAKSPVGMRM
                     RNAFLNETLKREQPFVMSVPASEADPSYPEDETILVQGIIDAWFPEGDEIVLVDYKTD
                     RVKEAGELKKRYEKQLAYYQQALERTTGKKVKEKIIYSLALDEELVL"
     gene            1951..2652
                     /locus_tag="PLN77_09235"
     CDS             1951..2652
                     /locus_tag="PLN77_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM44485.1"
                     /translation="MIRKIVQIEEDRCTGCGLCAHACHESAIAMVDGKAKLIRDDYCD
                     GFGDCLPVCPADAISFVEREALPYDEEAVKENQRKAKEAAGQENTGAGKAGENSWAID
                     RADAQENSAAVPSRLRQWPVQIKLVQERASMFAGADLLVAADCTAYAYGNFHQDFIRD
                     HVVLVGCTKLDDIDYSEKLARIFMRNDIKSITVARMEVPCCAGMERAVKMALKESGKK
                     IPVKTVVISTEGEIL"
     gene            2771..3337
                     /locus_tag="PLN77_09240"
     CDS             2771..3337
                     /locus_tag="PLN77_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44486.1"
                     /translation="MSMTKIQKELLGALIGLARSVDGRGEAAKGESQEFSSGDSNGHR
                     PDKATHEILLGGICAANPEKELREEEGKSWIVRVEAEKNRLNPDCAVCKGKCGRTDNY
                     DLEEMETDEPSDRARKEQILAELFPMAEKLQQLRKETKEQNEELQGAVNFLYKALFAI
                     GNYVGEKSLQSVVQEMNNLFVKLENVLK"
BASE COUNT         1090 a          710 c         1151 g          785 t
ORIGIN      
        1 gtacttttcc acgcaggaaa ttgttacggt gctgaattat ctgcatctgt gtgataatcc
       61 atgtcaggag attccattta cggccattct gcgttccccc atctgcggat gcacagacac
      121 ggagcttgcg gcggtgcgtt gtgtggataa agatgtgaaa atttacgagg catgtggaaa
      181 atatgcggcg atgggggatg atgaagcact ccgggagaag ctgaggaggt tccttgcaca
      241 gctcgaaacg ctgcgaagcc gcgtgccgta tacgccgatc catgagctga tcacacagat
      301 tctcgaggag acgggctacg gcggctatgc atctgcaatg ccgggtggtg tgcagcggcg
      361 ggcaaatctg gagatgctgg tggaaaaggc ggtggaattt gaggcgacga gttaccgtgg
      421 actgttcaac tttatccgtt atatcgagca gcttcagaag tatgaagtcg atttcggcga
      481 ggtgaatatt tacggggaga gcgcagacac ggtgcgtatc atgagtattc acaagagtaa
      541 aggactggaa ttcccggttg tgttcctctc gggcatgggc aaatcgttta accagatgga
      601 cagccgtgcg gcgcttgtac tccattcccg catgggtatc ggcgcggatg cgatcgatcc
      661 ggagcaccgc gtccgtcagg cgacactgcc gcggcagatc atccggcaga gcatgaagct
      721 ggaaaatctg gcggaggagc tgcgggtgct ttacgttgcc atgacgcgcc cgaaggagaa
      781 gctgattatg acgggcatgg cgtcgaagct ggagagccgc gtgaagcagt gcgcggcaca
      841 tgcggggcgg caggaggagc tgctgtcgta tcagacattg agcagtgcat ccgggtatct
      901 cgactggatt ttgccggcat tttcgagaaa ccggtgtttt gacgggctgt atgaacaatt
      961 cggtctggca gggaatgcgg acaatccgct gtatgcagag aaaattccgg taacgatccg
     1021 ccttgtatca ccggcaggac ttgttgaggg agaggtgctg catcagatgg agcgcgcggt
     1081 gaatgagcag aaaatcatgg aaattccgaa gcagccgccg agagaagcgg cgctgaagga
     1141 acttgtggag gagcggtttg cctaccagta tccgtatgag aaggaaaaac agattccggt
     1201 aaaggttacc gtttcacagc tgaagaaggc cgggatggag gaatccgaga tcggaaaaga
     1261 gctgttcgcg caggaagaac tcgtaccaat tgtgccgggc tttctgaaaa aggatcatgt
     1321 tgcggctctg acgggctctg accgcggaac ggcgtaccac cgcctgctgg aatgtctcga
     1381 ttaccgcaga gcgtacagca tcacagagct gatggcacag acggaggaat tgattgcaga
     1441 aggaaagatg acgaaagaag cagcggaatg catttatttc aaatcgatcc tcgctttcgc
     1501 aaaaagtccg gttggaatgc ggatgcggaa tgcctttttg aatgaaaccc tgaaacggga
     1561 gcagccgttt gtgatgtccg ttccggcaag tgaggcagat ccgtcctatc cggaagatga
     1621 aaccattctc gtgcagggaa tcattgatgc atggttcccg gagggggatg agattgttct
     1681 tgtggactac aaaacggacc gtgtgaaaga agccggggag ctgaaaaagc ggtatgaaaa
     1741 acagctcgcc tactaccagc aggcactgga gcggacgaca gggaagaaag tgaaagagaa
     1801 aattatttat tcgctggcac tggatgaaga gttggtattg taggaaaaaa ctactagaaa
     1861 aaacaactgt caaaacaggg ataaagtatg atatcatagt tgagaaacaa acaggtattg
     1921 agaaataaag agaaaaaagg agattcagcg atgattcgaa aaattgttca gatagaggaa
     1981 gacagatgta caggatgcgg gctttgtgcg catgcctgtc atgaaagtgc gattgcgatg
     2041 gttgacggga aggcaaagct gatccgggat gactactgcg acggattcgg cgactgcctg
     2101 ccggtttgtc cggcagatgc gatttcgttt gtggagcggg aggcgctgcc gtatgatgag
     2161 gaggcggtaa aagagaacca gcggaaggca aaagaggcgg ccggacagga gaataccgga
     2221 gcaggaaaag caggtgaaaa cagctgggcg attgaccgtg cagatgcgca ggaaaattct
     2281 gcagcagttc cgtcccggct ccgccagtgg ccggtgcaga tcaagctggt gcaggagcgg
     2341 gcttcgatgt ttgcaggagc agaccttctc gttgcggcgg attgtacagc gtatgcgtat
     2401 ggaaacttcc accaggattt tattcgggat catgtcgttc tcgtaggctg tacaaaactt
     2461 gacgatatcg attattccga gaaacttgcg cggattttca tgcggaatga tattaagagt
     2521 atcacggttg cacgcatgga ggttccgtgc tgcgcaggaa tggagcgtgc cgtgaagatg
     2581 gcactgaaag agagtggaaa gaagattccg gtgaaaaccg tggtgatttc tacggagggg
     2641 gaaattttgt agaaaatcag atatagtaat tagcgtgtgc ccttaaaagt atttttacaa
     2701 ttggtacatt ctgctttgtg gaatatgtaa gacgaaaaaa atgaaaagct cggtaagagg
     2761 tattagaaaa atgagtatga cgaagataca aaaggagctt cttggtgcgc tgatcggtct
     2821 ggcccggtcg gtagatggaa gaggagaagc agcaaaaggt gaatcgcagg agttttccag
     2881 cggtgacagc aatggtcacc gcccggacaa agccacgcat gagattctgc ttggcggaat
     2941 ttgtgcggca aatccggaaa aagagctgag ggaagaagag gggaaatcct ggattgtacg
     3001 cgtggaggca gaaaagaacc gtctgaatcc ggattgcgca gtgtgcaagg gaaaatgcgg
     3061 cagaacagat aactatgatc tcgaagaaat ggagacagat gaaccgtcgg accgggcacg
     3121 aaaagagcag attcttgcgg aattgtttcc gatggcagaa aaactgcagc agcttcggaa
     3181 agaaacaaaa gagcaaaacg aagaattgca gggggcggtt aattttttgt ataaggcatt
     3241 gtttgcgatt ggaaactatg tcggggaaaa gagtctgcag agtgtggtgc aggagatgaa
     3301 taaccttttt gttaaattgg aaaatgtatt gaaataagta gaaaataaga aaaatcctct
     3361 gtccggagtg tataaaaaac cccggaaaca ggggattttt tgcgttctgc gccaagtgca
     3421 taaaacgtat tatcgcgttt tcacaaagtt ttgcaaaagc agacaaaatt gcagacaggc
     3481 gcttgcaaca gaaaaatgaa taggttataa cattataaca aggcggcagg gagagccaat
     3541 acaaagaaat gagattctag tggcatataa acagaaagaa tacagggaaa agatgaaaag
     3601 ggacggaagc gggatggagc agaaaaaata tatccttctg gatgttggcg gaacggaaat
     3661 caaaggaagt gtgtcggaca gaaatggagt aagaacaaaa atccggaaat ttccagcaca
     3721 ggcgcagaag agcaca
//
LOCUS       DAOMUF010000192         3735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6956_length_3735_cov_2.747011, whole genome shotgun sequence.
ACCESSION   DAOMUF010000192 DAOMUF010000000
VERSION     DAOMUF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6956_length_3735_cov_2.747011"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PLN77_09245"
     CDS             <1..827
                     /locus_tag="PLN77_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRM44487.1"
                     /translation="GEVMDIAQGTCFRDPVSIIAGEYEDAKDSDIVIITSGIARKPGQ
                     TRIELTQTNVNILKSITPEIVKAAPNAIYIIVSNPVDIMTYVFTKISGLPENQILGSG
                     TMLDSARLRCGLSEHLNIAQKNIHAYVFGEHGDTSFIPWSGAYVSGVSLDEYYETVEK
                     MGRNVTKIDKDAMLEYVRTSGGQVIANKGATFYAVSVAVCKLVATILSSSDSVATVSS
                     MMHGEYGIEDVCLSTLTLVGPKGVQGKIPMRMTKEEVEKLKASADALKAVIAQIEH"
     gene            1034..1831
                     /locus_tag="PLN77_09250"
     CDS             1034..1831
                     /locus_tag="PLN77_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HRM44488.1"
                     /translation="MTYSYFPGCTLKNKAKDLDHYARECAKVLGFELEEIENWQCCGA
                     VYPQASDEIATKLSSVRALADAKEKGQDLITVCSACHHVIKRVNDDMANVEDIQTKVN
                     NYMALSEPYLGETKVLHYLEVLRDVVGFDKIKEKVVNPLKGRKIGAYYGCMLLRPSTT
                     MQFDDPENPTIIEDFIKALGATPVVYPMRNECCGGYISLKEKKMASNMVDQIMASASY
                     KGAEELITACPLCMYNLRNNGTKEGLPVTYFTELLAEALGIKEEVQA"
     gene            1828..2211
                     /locus_tag="PLN77_09255"
     CDS             1828..2211
                     /locus_tag="PLN77_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRM44489.1"
                     /translation="MKIVTSDEIRAIAGVDTRKCMKCGKCSASCPAYNEMDIRPHQFV
                     SYVQNGDVEALMNSKSIWKCLSCFACVERCPRDVKPAKLIEAVRLSVIRQKGENYLSP
                     DEIPALLDDETPQQLLMSAFRKYRK"
     gene            2245..>3342
                     /locus_tag="PLN77_09260"
     CDS             2245..>3342
                     /locus_tag="PLN77_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM44490.1"
                     /translation="MQRIGVFVCHCGTNIAATVDVKKVVDVISKEPGVVHAEDYQYMC
                     SETGQQKVIDAIQNEHLTGVVICSCSPRMHEGTFRKAAEKAGLNPYQLEIANIREQCS
                     WIHKDMEEATEKAVILARAAVAKVMLDTPLTPGESRVVKRALVIGGGIAGIQTALDIA
                     DAGYEVDIVEKTPSIGGRMSQLDKTFPTLDCSSCILTPRMGEVNAHEKINIYTYSEVE
                     SVGGFVGDFKVDIRKKARYVDMDKCTGCGLCQEKCPSKKNLSEFNRGLSTRTAIYTPF
                     AQAVPNVPVIDTANCIHFKTGKCGICSKVCAAGAVNYDQKDEVITREYGAIVVATGFD
                     TINLDKFDEYYYNQSKDVITSLEMERLMNAAG"
     assembly_gap    3343..3378
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3379..>3735
                     /locus_tag="PLN77_09265"
     CDS             <3379..>3735
                     /locus_tag="PLN77_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="disulfide reductase"
                     /protein_id="HRM44491.1"
                     /translation="SYCSKICCMYTAKQAMLVRDHYPDVNVHVFYIDVRTPGKNFDEF
                     YRRAVEEFSVDYIKGQVGKVSEAPNGRLLVQGSDLLDNRQIKMEADLVVLATAIEPSK
                     DARKLATMLTASMDTN"
BASE COUNT         1100 a          840 c          910 g          849 t
ORIGIN      
        1 agggagaggt tatggatatc gcgcagggta cctgcttccg cgatccggtc tccatcatcg
       61 caggtgagta tgaggatgcc aaggactctg atatcgtaat tattacttcc ggtattgcaa
      121 gaaagcctgg ccagacccgt attgagctga cccagaccaa cgtaaacatc ttaaaatcca
      181 tcacaccgga aatcgtaaaa gcggctccga atgccatcta catcattgta agcaacccgg
      241 ttgatatcat gacctacgtg tttacaaaga tctccggact tccggaaaac cagatccttg
      301 gttctggtac catgctggat tccgcgagac ttcgctgcgg actgtctgaa cacctcaaca
      361 ttgcccagaa aaacatccac gcttacgtat tcggtgagca cggcgatact tccttcattc
      421 cgtggagcgg cgcttacgtt tccggtgtca gtctggacga gtactacgag accgtagaaa
      481 aaatgggcag gaacgtaacc aagatcgaca aagatgctat gctggagtac gtaagaactt
      541 ccggcggaca ggttattgca aacaaaggtg ctactttcta tgcagtatct gttgcagtat
      601 gcaaactggt agcaaccatt ctttcttctt ccgattctgt agcaaccgta tcttccatga
      661 tgcacggtga gtacggcatc gaggatgtat gcttaagcac actgaccctc gttggaccga
      721 aaggcgtaca gggcaagatt ccgatgcgta tgacaaaaga ggaagtagaa aaactcaaag
      781 caagtgccga cgcattaaaa gcagttatcg cacagatcga gcactaagag cgggcaataa
      841 gctcaaaaca taaaatttat ggcagtggca gaaagtgcga aagagcggta tctcccactg
      901 ccttaacgtg cgcctgaaga aattttccgc cgggtgtaca taaaaagagt accggaaact
      961 tttccggtgc cggacagaag ataccgcagg aaaaatggaa accaacagga gcaagtaaga
     1021 aaggagcaat tccatgacct acagttattt tccgggctgt acgctgaaga acaaagcaaa
     1081 agatctggat cactatgcca gagagtgcgc aaaagttctg ggatttgaac tggaagaaat
     1141 tgaaaactgg cagtgctgcg gagctgtgta tccacaggca tccgatgaaa tcgccacaaa
     1201 actttcttcg gttcgcgcct tagcagacgc caaagaaaaa ggacaggatc tgatcacggt
     1261 ctgttccgcc tgtcatcatg taatcaaacg tgtcaacgat gatatggcga atgtagaaga
     1321 cattcagacc aaagtaaaca actacatggc tctttccgag ccatatcttg gcgaaacgaa
     1381 agtacttcat tatctggaag tgctgcgtga cgtcgttgga tttgacaaga tcaaagagaa
     1441 agtggtaaat ccattaaaag gaagaaaaat cggcgcatat tacggctgta tgctgcttcg
     1501 cccaagcacc acaatgcagt tcgatgatcc ggagaacccg acaatcattg aagattttat
     1561 caaagccctt ggtgcaacac cggtcgtata tccgatgaga aacgagtgct gcggcggcta
     1621 catatccctc aaagaaaaga aaatggcatc caacatggta gaccagatca tggcgtccgc
     1681 atcctacaaa ggagcagaag aactgatcac tgcatgtccg ctttgcatgt acaacctgcg
     1741 caataacgga acaaaagaag gtcttccggt tacctacttt accgagcttc ttgcagaagc
     1801 actcggcatc aaagaggagg tgcaggcatg aaaattgtaa catccgatga aatccgtgcc
     1861 atcgccggcg tggataccag aaaatgtatg aaatgcggta aatgttccgc atcctgtcct
     1921 gcttataacg aaatggacat ccgtccgcat cagtttgtat cttacgtaca gaacggcgat
     1981 gtggaagcac tgatgaactc caagtccatc tggaaatgcc tctcctgctt cgcatgcgta
     2041 gagcgctgtc caagagacgt aaaacctgcc aaactgatcg aggcagtccg tctttctgtc
     2101 atccgccaga aaggtgagaa ttatctctcc ccggatgaaa ttccggcact tctggacgat
     2161 gagactccgc agcagctgct gatgagtgca ttccgtaaat atagaaagta aaaaacaacc
     2221 catccacaga aaggtgagag aaaagtgcaa agaataggag tatttgtctg ccactgcggt
     2281 acaaatattg cggctaccgt tgatgttaaa aaagttgttg atgtcatcag caaagagccg
     2341 ggtgttgtac atgcagagga ttatcagtat atgtgttccg aaacaggtca gcagaaagtg
     2401 atcgatgcca ttcagaacga gcatctgacc ggcgttgtta tctgttcctg ctctcctcgt
     2461 atgcatgaag gaacgttccg caaagcagca gaaaaagcgg gtctgaaccc gtatcagctg
     2521 gagattgcaa acattcgtga gcagtgttcc tggattcata aagatatgga agaggctacc
     2581 gagaaggccg taattctggc ccgtgcagcc gttgctaaag ttatgctgga taccccgctt
     2641 accccgggcg agagccgcgt tgtaaaacgt gcgctggtga tcggaggcgg tatcgcaggt
     2701 attcagacag cactggatat cgcagatgca ggctatgaag tagatatcgt agagaagacc
     2761 ccgagtatcg gcggaagaat gtcccagctt gataaaacat tcccaaccct cgactgttct
     2821 tcctgtatcc tgactcctcg tatgggagag gtaaacgcgc atgagaagat caacatctat
     2881 acctacagtg aagtagagag cgttggcgga tttgtcggtg atttcaaagt agatatccgc
     2941 aaaaaagcaa gatacgtaga tatggacaaa tgtaccggat gtggtctctg ccaggagaaa
     3001 tgtccatcta agaaaaactt aagtgagttc aaccgtggac tgtccacacg tactgcaatt
     3061 tacacaccgt tcgcacaggc agttccgaat gtaccggtca ttgatactgc aaactgtatc
     3121 catttcaaaa ccggaaaatg cggtatctgt tccaaagtct gcgcagcagg tgcagtaaat
     3181 tacgatcaga aagatgaagt aattacaaga gagtacggtg ctatcgttgt tgcaaccggt
     3241 tttgatacca tcaatctgga taagtttgat gaatattatt acaaccagag caaagacgtt
     3301 atcacttctc ttgagatgga acgtctgatg aacgctgccg gcnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnga gctactgctc caagatctgc tgtatgtaca ccgcaaaaca
     3421 ggccatgctg gtgcgtgacc attatccgga tgtcaatgta catgtcttct acattgatgt
     3481 tcgtaccccg ggtaagaact tcgatgagtt ctatcgccgt gcagtagaag agtttagtgt
     3541 agattatatc aagggtcagg taggaaaagt atctgaggct ccgaacggac gtcttctggt
     3601 acagggatcc gaccttctgg acaaccgtca gatcaagatg gaagcagacc ttgttgtcct
     3661 tgcaacggca atcgagccga gtaaagatgc aagaaaactg gctaccatgc tgactgcaag
     3721 tatggatacc aacga
//
LOCUS       DAOMUF010000193         3719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_6998_length_3719_cov_4.257369, whole genome shotgun sequence.
ACCESSION   DAOMUF010000193 DAOMUF010000000
VERSION     DAOMUF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6998_length_3719_cov_4.257369"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..606
                     /locus_tag="PLN77_09270"
     CDS             322..606
                     /locus_tag="PLN77_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM44492.1"
                     /translation="MVSEKVTIVNPSGLHLLNAVNLSNSVSRYKSSMSFTYENNGMQG
                     TVNLKSILSIVAAGIKQGETIEICSSGEDEEEALRAAVAAIKSGLGETPE"
     gene            909..1121
                     /locus_tag="PLN77_09275"
     CDS             909..1121
                     /locus_tag="PLN77_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HRM44493.1"
                     /translation="MARQKKIDLEHMTEKEKMKYEIAGELGLLERVLNDGWRSLTAKE
                     TGRIGGLVTRRKRQMAAEGKNENEEK"
     gene            1317..3104
                     /locus_tag="PLN77_09280"
     CDS             1317..3104
                     /locus_tag="PLN77_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRM44494.1"
                     /translation="MEAKKEKKYMSDIHLEDYTEQYETGFATVDTMIFEGGRREELLN
                     GGWHYAVDQYDTCLRQKWYKERYRDEKGFTVPIDYSFDEWPVMQLPCSWNTIDPMYLL
                     YEGSMVFTRKFSYIAEREETVFLKVGAANYLCRVFLNGKYVGMHRGGSTPAFWNITEY
                     LKAENRIVLAVDGTRRPEQVPTENTDWFNYCGVYRDIALIRVPKCHIKTFKIALVPDG
                     TFGHVMAKVTLSEKITAKAELVIEELGVSRKIQLENGAGEVVFDAKPELWTPEKPKLY
                     DVKVTCGTDTVSDRVGFREIRVNGRDILLNGEPVFLRGISCHEDSVENGKGLTREERI
                     ENIRIAKELGCNFMRLAHYPHNEEMAKLADELGLLLWEEIPVYWAIRFEREKTYEDAQ
                     NQLRELINRDWNRASVIIWSVGNENADTDERLKFMSALAECAHREDETRMVSAACLVN
                     AAKNKIEDRLMEYLDIIGINEYCGWYTPDFAMLPALMENSQPDKPVIVTEFGADALPH
                     HHGTISDKGTEECQADVYEKQIATLRNIDYIKGMTPWILYDFRCPRRTSLIQKYYNRK
                     GLLSEDKKYRKPAFYVLQKFYEELKRKEQ"
     gene            complement(3290..>3719)
                     /locus_tag="PLN77_09285"
     CDS             complement(3290..>3719)
                     /locus_tag="PLN77_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44495.1"
                     /translation="TAIYGLDGMSIVNSPLWRPAGVMVMFQVSMISDEDILKLKDLPI
                     WFTHAKTDPVVVPDDFVVPTYERLAKVNPNAHFTYWDKVLDHTGTQKNADGTPFEYIG
                     HWSWIPMLNDECVLDYDGKPVITDGKETPILEWMAAQKKA"
BASE COUNT         1120 a          782 c         1019 g          798 t
ORIGIN      
        1 gccgcatttc cactccttaa aaggagctgc cttctgctat ccgctgtcgt gggtgatcac
       61 aacgattgcg tactttattt attacctgtt cttcagccag ctgagtaagc ggaaagaatt
      121 gaggagtatc taacagaatg ttcaaactgt attaacaaaa ctgtgctatc atgaaaatat
      181 cagtgtcaaa aggtcaaaaa accgatgcag gcaaagcttg caggaggatt tttgaactcg
      241 ggacacggca aaaaatgagt aagtagccat ttttcaaaga aaaatgagcg gattacaaag
      301 gaaagacagg tggaacgtaa gatggtaagt gaaaaagtaa cgattgtcaa tccgtcaggt
      361 ctgcatcttt taaacgcggt aaatttgtca aacagtgtaa gccggtacaa atcttccatg
      421 tcgtttacct atgaaaacaa cgggatgcag ggaacggtta atttaaagag tattttgagt
      481 atcgtagctg caggaatcaa gcagggagaa accattgaga tctgcagcag cggggaggat
      541 gaagaggagg cacttcgggc ggctgttgcc gcaattaaaa gcgggctcgg agagacgccg
      601 gagtgaggag ataggaacat agaatagaaa taaagaaaag gacctcgctt tttgaaaaaa
      661 cggaatggga aaccggaccg tatttcaaaa agcgaggttt ttttgtggcg gcagataaaa
      721 gatccctgcc ttcggggggg aatttcgaaa gcagggatga aaagataagc tgcgaagcag
      781 ctcttcggct atgagcaagc agcgaagcgg attgcgaata tccgtttgac aacaggaggt
      841 ataaggccgg aaaaaagtgt ctacactctg gataaagcaa aaatgcagag aaaaggagtg
      901 cagaaaaaat ggcaaggcag aagaaaattg atctggaaca tatgacggag aaggagaaaa
      961 tgaaatacga aatcgcaggt gagctggggc ttttagaacg cgtgctgaat gacggctggc
     1021 gatcactcac ggcaaaagaa acgggacgga tcggtggcct tgtgacacgg aggaagcggc
     1081 agatggcggc ggaaggaaag aacgaaaacg aagaaaaata agcggtaccg caatttttgc
     1141 aatatcctca acagttttga ccatagattt ttcctgtgaa agcgttataa taaaaacaag
     1201 gaatgggtgg tgccgcgcca aagtccgtgt ctgcacgaga cccggcgact gccgcgatac
     1261 cctgagaaac tcgcagagcg tgtttcggat cgtttggaga agtgagggaa aagtttatgg
     1321 aagcaaaaaa agagaaaaag tatatgtcgg atatccatct cgaggattat accgaacagt
     1381 acgaaacggg atttgccaca gtggacacga tgatatttga agggggaaga agagaggagc
     1441 tgctgaacgg cggctggcat tatgcagtcg atcagtacga tacctgtctg cgccagaaat
     1501 ggtacaaaga gagataccgc gacgaaaaag gcttcactgt gccaatcgac tattcctttg
     1561 acgagtggcc ggtgatgcag cttccgtgca gctggaatac catcgatccg atgtatctcc
     1621 tgtatgaggg aagcatggtc tttacgagaa aattttccta tatcgcagaa agagaggaaa
     1681 cagtctttct gaaggtgggc gcagccaatt atctgtgccg cgttttctta aacggaaaat
     1741 acgtcggtat gcatcgcggc ggatcgacac cggcgttctg gaatatcaca gaatacctga
     1801 aagcggagaa ccgcatcgtg cttgctgttg acggaacgag acggccggag caggtgccga
     1861 ccgagaatac cgactggttc aactactgcg gcgtataccg cgatatcgca ctgatccgtg
     1921 ttccgaaatg ccatatcaaa acttttaaaa ttgcgcttgt gccggacgga acatttggac
     1981 atgttatggc gaaagtgaca ctgtcggaaa aaatcacagc caaggcagag cttgtcatcg
     2041 aggagcttgg cgtgagccgg aaaatccagc tggaaaacgg agccggagaa gttgtttttg
     2101 atgcaaaacc ggagctttgg acaccggaaa aaccgaagct gtatgatgta aaagtaacct
     2161 gtggaaccga taccgtcagc gatcgggtcg gctttcgtga aattcgcgtg aacggcagag
     2221 atattctgct gaacggagag cccgttttcc tgcgtggtat cagttgccac gaagacagcg
     2281 tggaaaacgg aaaaggcctg acaagagagg aacggatcga aaatatccgc attgcgaaag
     2341 aactgggatg caacttcatg cgccttgcac attacccgca taacgaagaa atggcaaaac
     2401 tcgccgacga gcttgggctt cttttgtggg aggaaattcc ggtttactgg gcaattcgtt
     2461 ttgaacgtga aaaaacatat gaggatgccc agaatcagct ccgcgaactc atcaaccgtg
     2521 actggaaccg cgcgagcgtg atcatctggt ctgtcggaaa tgagaatgcc gatacggatg
     2581 aacggctgaa attcatgagc gcactggcag aatgcgcgca tcgggaggat gagacgcgaa
     2641 tggtgtctgc ggcctgcctg gttaatgctg cgaaaaataa gattgaggac cgtctgatgg
     2701 aataccttga catcatcggc atcaacgagt actgcggctg gtacaccccg gattttgcga
     2761 tgctgccggc actgatggaa aacagccagc cggacaagcc ggttatcgta acggagttcg
     2821 gcgcagacgc gctgccgcat caccatggaa ccatctcgga caaaggaacc gaggagtgcc
     2881 aggcggatgt gtacgaaaaa cagattgcaa cgctgcggaa cattgactac atcaagggca
     2941 tgaccccctg gattctctac gacttccgct gcccgcgcag aacaagcctg atccagaaat
     3001 attataaccg gaaggggctg ctctctgagg acaaaaagta ccgcaaaccg gcattttatg
     3061 tgctgcagaa attttatgaa gagctgaaaa gaaaagaaca gtaggggcaa gagcttttca
     3121 aaaagatcag ccgacttgca cggttgatga tatcaaacgg aacagagaaa aaaagacaag
     3181 aacagacaaa aacagatggt atgaaatcag ctttgaagtc cgatttcata ccatctgttt
     3241 ttttcactgc aacaatccgc cgtaggcgga gaatccggca agtgctgtgt tatgcttttt
     3301 tctgtgcagc catccactcc agaatcggag tctcttttcc gtctgttata accggttttc
     3361 cgtcgtaatc gaggacacac tcatcgttca gcatcggaat ccaggaccag tggccgatgt
     3421 attcgaatgg ggtgccatcc gcgtttttct gtgttccagt gtgatcgagt accttgtccc
     3481 agtaggtgaa gtgtgcattc ggattgacct ttgccagacg ttcataggtc ggaacgacaa
     3541 aatcatccgg aacgacaacc gggtcggttt ttgcgtgtgt aaaccagatc ggcagatctt
     3601 tgagcttcag aatatcttca tcggaaatca tcgacacctg gaacatgacc atcaccccgg
     3661 caggccgcca cagcggagaa ttcacgatcg acatgccgtc aagaccgtat atggctgtg
//
LOCUS       DAOMUF010000194         3716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7010_length_3716_cov_3.582081, whole genome shotgun sequence.
ACCESSION   DAOMUF010000194 DAOMUF010000000
VERSION     DAOMUF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7010_length_3716_cov_3.582081"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    282..291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(394..1158)
                     /locus_tag="PLN77_09290"
     CDS             complement(394..1158)
                     /locus_tag="PLN77_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRM44496.1"
                     /translation="MNLYEAIFVRKSVRNYCFDTLPPQTLDKIWEHYKEMPALFSGIG
                     VDMAILDNRKGQERMLSMFSVKAPYYMAFYSEESERYLMNVGYIMEQMVLYLCSIGLG
                     TCFIGSNRVKKAELEKNGKRLVGIVAFGKSHGSHTRRQSEAKRLPLEDLCVFKEVPRQ
                     WMTQMLEAARLSPSSMNSQPWRFVVYDNRIHIFSKKHSVEKLRKWDEVNFGIMFANMM
                     VAAEELWLDVDLIRLGDISQKNFSNNQYVLSAILKA"
     gene            complement(1498..3306)
                     /gene="pepF"
                     /locus_tag="PLN77_09295"
     CDS             complement(1498..3306)
                     /gene="pepF"
                     /locus_tag="PLN77_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HRM44497.1"
                     /translation="MAEIKLKKREEMDPAYCWDLSSLFGSDEEFEKTEQELAKKIEAF
                     RAKQGTMKESAEALYDVLSGLDDISRVFSSLYVYASQKYHQDMGNQKYQGYAGKMDSM
                     MAVLADASSFVEPEILELDEETVTEFEKAYEPLRLYDRILKENFRKKEHILSPEMEAV
                     LAKAGEMASSPDQIFSAFSNADLRFQPVTDEEENQVPLSHGTFVGFLQSKDRRVRKEA
                     FEKYYAEYEKHKNMLAATFGANLKQAAFFADVRKYPSALAASLDGGNIPVSVYDKLLE
                     AIHAKMPAMYRYVALRKKLLGVDELHMYDVYVSLTKEYEQKYTYEQAIEIVKKALAVL
                     GDDYVALLDKGFSERWVDVYENEGKKSGAYSWGSYDSHPYVLMSFNGNIDSVFTLAHE
                     MGHSLHSWYSNHTQPFTYAEYRLFVAEVASTCNEALLIRYLLKHAKEKEEKIFLLNYF
                     LDQFKGTVFRQTMFAEFEKRIHEKMAEEGTLTADGISELYLSINKEYFGPDMISDPQI
                     ALEWARIPHFYTPFYVYQYATGFSAAIAISSKILAGEPGIVEKYKQFLSGGCSMDPID
                     LLKICGVDMTKPEPVEEALDVFASYVEELEKLTAEA"
     gene            3509..>3716
                     /locus_tag="PLN77_09300"
     CDS             3509..>3716
                     /locus_tag="PLN77_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM44498.1"
                     /translation="MPWNLDSSRPIYLQIIERVQMDIITGRYQPGDKLPSVRDLAQEA
                     AVNPNTMQKALSELERSGLIYSQRT"
BASE COUNT          909 a         1092 c          713 g          992 t
ORIGIN      
        1 cgcctcctgg cggatccaga cgctttaccc ccgtccccac cgtcagacct cgcgtggctt
       61 cgtacctgcg gtgcagcggg catagaaaaa gagggcaaga actgtgagcg cagaaggggc
      121 ccggaaaaat gggatgacct ctggtgcaca ataccagagg tcaaaacgca ctgagtcggg
      181 aaaccatcag gtttcctgac gaattgcgtt gattctcatt tttccgggcc ccttccgcgc
      241 cacaagttcc gccctctttt tctcgcccgc cgcaccgcag gnnnnnnnnn nggcggatcc
      301 agccgcttta tccccgtccc caccgtcaga cctcgcgtgg cttcgtacct gcgatgcagg
      361 tacgaagccg cacgaggtcg tccgtattca cctctacgcc ttcagaatcg cgctcagcac
      421 atactgatta tttgagaagt ttttctgcga aatatccccg agtctgatca ggtcaacatc
      481 cagccacagc tcctctgctg ccaccatcat atttgcaaac ataatcccga aattcacctc
      541 atcccacttc cgaagcttct ccacactgtg cttcttcgag aaaatatgaa tcctgttatc
      601 atacaccaca aatctccacg gctggctgtt catactggat ggcgacagtc tcgccgcttc
      661 cagcatctgc gtcatccact gtcgcggcac ctctttgaac acacaaagat cctccagcgg
      721 cagtctcttt gcttcacttt gccgtctcgt atgcgaacca tgactcttac caaaagcgac
      781 gatccccacc agccgctttc cgttcttctc aagctccgcc tttttcacac gattacttcc
      841 gataaagcag gttcccagtc cgatactgca cagatacagc accatctgtt ccataatata
      901 acccacgttc atcagatatc gttctgactc ctccgaataa aaagccatat aatacggcgc
      961 cttcacactg aacatactga gcattctctc ctgccctttt ctgttgtcca gaatcgccat
     1021 atccacaccg atgcccgaaa acagtgccgg catctcctta taatgctccc aaatcttatc
     1081 caaagtctgc ggcggaagcg tatcaaaaca ataattcctt actgattttc taacaaaaat
     1141 agcctcatat aaattcatac tctcaccctt acttcatctg ctatccgtct gtttttgtta
     1201 cagttcactg ctgaatgaac agtaatattt ctttcatagc cggccgcggc ccgctcccca
     1261 tttttacagg agcaaagccg cattccgaaa aatttacgtt taatcttccg cattcgacag
     1321 aaatacggaa attctccctc ttaatcgcaa atatttttca atttccagct catccatttt
     1381 tactctgtaa taatattgta acatatccaa aaactttttc aagggaaaaa cgcgccaaat
     1441 tttgtatatt tttcacacat atttcccatc aaatttatgc aactttaaaa cctgtgctta
     1501 cgcttccgcg gtcagcttct ccagctcctc cacataagaa gcaaacacat ccagcgcctc
     1561 ttccaccggc tccggcttcg tcatatcgac gccgcagatc ttcaaaaggt cgatcggatc
     1621 catcgagcag ccgccgctta agaactgttt gtacttctcc acgatacccg gctcccctgc
     1681 caggatcttg ctgctgatcg caatcgccgc cgaaaatccc gttgcatact gatatacata
     1741 gaacggtgta taaaaatgcg gaattctcgc ccattccagc gcgatctgcg gatccgaaat
     1801 catatccggt ccgaaatact ccttgttgat cgacagatac agctccgaaa taccgtccgc
     1861 cgtcagcgtg ccttcctctg ccatcttctc atgaatccgc ttctcaaact ccgcaaacat
     1921 cgtctggcgg aacaccgttc ccttaaactg atccaggaaa taattcagca ggaaaatctt
     1981 ctcctctttt tctttcgcat gcttcagcag atagcggatc agcagtgcct cgttgcaggt
     2041 tgatgccacc tcagcgacaa agaggcggta ctccgcatac gtaaacggct gcgtatggtt
     2101 cgaataccag gaatgcagcg aatggcccat ctcatgcgcc agtgtgaaga ccgaatcgat
     2161 atttccgtta aagctcatca gcacatacgg atgggaatcg taagaacccc aggagtaagc
     2221 accgctcttt tttccttcgt tctcataaac atccacccag cgctccgaaa agcccttgtc
     2281 cagcagtgcc acataatcat ccccgagcac ggcaagcgct ttcttcacga tctcgatcgc
     2341 ctgctcatac gtatatttct gctcgtactc tttcgtcaga gaaacgtaaa catcatacat
     2401 atgaagttca tccacaccga gcagcttctt acgcaatgcc acgtagcggt acatcgccgg
     2461 catttttgca tgaatcgcct cgagcagctt gtcgtaaacc gaaaccggga tatttccgcc
     2521 atccagcgat gcagccagtg cagatggata ttttcttaca tctgcaaaga atgcggcctg
     2581 tttcagattg gcaccgaacg ttgcggcgag catgtttttg tgtttttcat actccgcata
     2641 atacttctca aacgcttctt ttctgacccg gcggtccttc gactgtaaaa atccgacgaa
     2701 ggtgccgtgg ctcagtggaa cctgattttc ctcttcatcc gtgaccggct gaaatctgag
     2761 gtctgcgttc gaaaatgccg agaaaatctg atccgggctg gatgccatct ctccggcttt
     2821 tgcaagaacc gcctccatct ccggcgagag aatgtgctct ttcttgcgga agttttcttt
     2881 taatatgcga tcgtacagac ggagcggctc atatgctttt tcaaattctg taaccgtctc
     2941 ctcgtcaagc tccaggatct ccggctctac gaaagaagag gcatccgcaa gaacagccat
     3001 catgctgtcc atctttccgg catagccctg atacttctga tttcccatat cctggtgata
     3061 tttctggctt gcatagacat acaggctgga aaagacgcgg ctgatgtcat ccagaccgga
     3121 aagaacgtca tacagcgctt ctgcactctc tttcattgtt ccctgttttg cgcggaacgc
     3181 ttctattttc ttcgcaagct cctgctctgt tttctcaaat tcttcatcac taccaaacaa
     3241 actcgaaaga tcccagcagt acgccggatc catctcttct ctttttttca gtttaatttc
     3301 tgccatattt ctcccttcat ccatcttttt agacggaacc atttctgcgg tttttcttct
     3361 aatactaatg tgccgcactt acgcttagtt taccacaatt taacctctaa atctaaaatt
     3421 ttttcttaat tttcttgaca aatccataaa ccgattgtat gattgtacta tcaaattaat
     3481 acagtagtac aaaaggagtg aatgccatat gccatggaat cttgattcct cgcggccgat
     3541 ctatctgcag atcatcgagc gggtacagat ggacatcatc accgggcgct atcagccggg
     3601 cgataagctt ccgtccgtcc gcgatctcgc gcaggaggcc gccgtcaacc cgaacacaat
     3661 gcagaaagcg ctttccgaac tggaacgcag cggactgatc tattcccagc ggacca
//
LOCUS       DAOMUF010000195         3643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7192_length_3643_cov_5.292642, whole genome shotgun sequence.
ACCESSION   DAOMUF010000195 DAOMUF010000000
VERSION     DAOMUF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7192_length_3643_cov_5.292642"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1482)
                     /locus_tag="PLN77_09305"
     CDS             complement(148..1482)
                     /locus_tag="PLN77_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44499.1"
                     /translation="MMNRINWTELKPQIQQVGRLSLPAILTQITTIAMQYIDSAMVGA
                     LGANASAAIGLVSSTTWLLSGTIYAVSAGFSVQVAHQIGGNHDKKARDVVRHGIASAL
                     VVSAVLCLIGLLISHPLPGWLGGAQKIRRNATLYFMMFAIMLPFSQLNSLMSSFLQCS
                     GDMVTPSILNAVMCVLDVIFNALLIPVFGVMGAGMGTMLACAVISLTGAWFCCVRNPR
                     LRLRRKEKTVFDAKILKTAFKIGVPVAVQEIAMNSAMVAATMLIAPLGSIAIAANSFA
                     VTAEGLCYMPGYGIGSAATTLVGRSYGAGNYKLAKRYGNICIAMGAVLMAITGGLMMI
                     FCPFVFSILTPDPQVRSFAAEVLRIELLAEPFFGASIVAAGALRGTGDTFVPSLLNLG
                     SIWVIRLGLAVLLITPLGLRGMWIAMAIELCIRGLLLLYRQHTSKYYKLPAA"
     gene            complement(1644..2480)
                     /locus_tag="PLN77_09310"
     CDS             complement(1644..2480)
                     /locus_tag="PLN77_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HRM44500.1"
                     /translation="MKILNLENENRKFTKSIENFHVMEYLQDSSVSPMTAMEEYYMSK
                     MNVRRRQVVIELDKEHSAIIQSGAMQWMGGHVQATAGIKGIGDLFGKAIKGAMTKESA
                     VKPEYVGDGYLVLEPTYKYIILVDVEKWGSSGMTIEDGIFLACDGRVKTKLTARKSIS
                     SAVLGGEGFFNLSLVGRGAVALESNVPEDELIEVELENDELKIDGNLAVCWSSNLDFT
                     VERSTKTLVGSAVSGEGLVNVYRGTGRVLMSPVAPTDSLLTATNTTQANPAVKNNLPP
                     EN"
     gene            2676..3245
                     /locus_tag="PLN77_09315"
     CDS             2676..3245
                     /locus_tag="PLN77_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRM44501.1"
                     /translation="MSTITVRDAKIEDAARILEIYAYYVEYTVISFEWDVPSLEEFEN
                     RMRDIMKKYPYLVIEQDGKIEGYAYAHAFVGRAAYDWSSELTIYLDPNARKGGLGRRL
                     YEELADRLKKMGILNLYACIGYPQVEDEYLTKNSAEFHAHLGFELVGTFHNCGYKFDR
                     WYDMVWMEKIIGEPHAGQPAVKPYSEVKE"
     gene            3242..>3643
                     /locus_tag="PLN77_09320"
     CDS             3242..>3643
                     /locus_tag="PLN77_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44502.1"
                     /translation="MSTITTYTRKHFDPVHPDPGLLCVEDFAHALPMICRGNGHVTTF
                     WSVGEHCICCAKEAAARGYSRRLVLACLLHDASECYMSDVPRPLKQEMPRYREIEDHL
                     LSVIYEKYLGSDLTAEEQRMMKQIDNDLLWYD"
BASE COUNT          918 a          897 c         1010 g          818 t
ORIGIN      
        1 agtcacagtc gaactatttt cgacatgtca gactccgtga aaataccgac tgtgaattga
       61 aacaagggat aaggtaaaat agaaagcgct tatttgaggc gggaatgaaa tctcaaataa
      121 gcgctttttc tatacgctgc cgccaagtca ggctgcgggc agtttataat atttcgatgt
      181 atgctggcgg tagagcagca gcaatccgcg gatgcagagc tcgatagcca tggcgatcca
      241 catgccgcgc aggccgagcg gggtgatgag aagcaccgcg agaccgaggc ggatgaccca
      301 gatgctgcca aggttcagaa ggcttggcac aaacgtatcg cctgttccgc ggagggctcc
      361 ggcggcaacg atggaagcgc cgaaaaacgg ctcggcgagc agttcgatgc ggagtacctc
      421 ggcggcaaac gagcgaacct gcgggtcagg agtcagaatg ctaaagacga acggacagaa
      481 gatcatcatc agacctccag tgatggccat caggactgcg cccatggcaa tgcagatatt
      541 tccgtagcgt ttcgccagct tgtaatttcc ggcgccgtag cttctgccga cgagcgttgt
      601 cgcggcggaa ccgatgccgt agcctggcat gtagcagagg ccttcggcgg tgacggcgaa
      661 cgaattggcc gcaatcgcaa tggagccgag cggggcaatc agcatggtag cagcaaccat
      721 ggcgctgttc atcgcgattt cctgtacggc aaccggcaca ccgattttga atgcagtttt
      781 cagaattttc gcatcgaaaa ccgttttttc cttccggcgg agacgcagcc gcggattgcg
      841 gacacaacag aaccaggcac ccgtgaggct gatgacggcg caggccagca tcgttcccat
      901 accggcaccc atgacaccga aaaccggaat caaaagagca ttgaaaatga catcgagcac
      961 gcacatgacg gcatttaaaa tactgggtgt caccatatca ccgctgcact gcaggaagga
     1021 cgacatcagc gagttgagct gggaaaacgg aagcatgatg gcaaacatca taaaatagag
     1081 cgtggcattc cgccggatct tctgcgcacc gccgagccac cccggcagcg gatgactgat
     1141 cagaagcccg atcaggcaca aaacagccga cacaacaagc gcggacgcga tgccatgccg
     1201 cacaacatcc cttgccttct tgtcatgatt tcccccgatc tgatgcgcta cctgaacaga
     1261 aaaaccggct gaaaccgcat aaatcgtacc gctgaggagc cacgtcgtcg acgaaaccag
     1321 tccgatcgcc gccgaagcat tcgccccaag cgcgccgacc atcgcggaat caatgtattg
     1381 catcgcaatc gtagtaatct gtgtaagaat cgcaggcaga cttaatcgcc cgacctgctg
     1441 aatctgcggt ttcagttcag tccagtttat tctattcatc attttctcca gaacatgtat
     1501 tttatcgtat ggtttttctt aagaatgtaa agataaatgc gtattttacg ctaacatgtt
     1561 ttgaagaaag tgtcaatcaa aaacgaggtt taatttgact tttttctatg cgtattaggc
     1621 ataggggcgg agaggattcg aatttaattt tccggcggaa ggttgttctt cacagcgggg
     1681 ttcgcctggg tcgtgttggt tgcagtcagc agggaatctg tcggggcgac cgggctcatg
     1741 aggacgcggc cggttccgcg gtagacgttg acgaggcctt cgccgctgac ggcggagccg
     1801 acgagggttt tggtggagcg ctctacggtg aaatcaaggt tggaggacca gcagacggcg
     1861 aggtttccat cgattttcag ttcgtcgttt tcgagttcga cttcgatcag ctcgtcctca
     1921 ggtacgttgc tttcgagggc aacggcgcct ctgccgacga ggctcagatt gaagaagcct
     1981 tcgccgccga ggacagcgga ggaaatgctc ttgcgggcgg tcagtttggt tttgactctg
     2041 ccgtcgcagg cgaggaatat gccgtcctca atcgtcattc cggaggagcc ccatttctcg
     2101 acatccacaa gaatgatgta tttgtaggtc ggttccagca cgagatatcc gtcgccgacg
     2161 tactccggtt ttacggcaga ttcctttgtc atggcgcctt tgatggcctt tccgaacagg
     2221 tcgccgatcc ctttgattcc ggcggtcgcc tgtacatggc cgcccatcca ctgcatcgca
     2281 ccggactgga taatcgcgga gtgctccttg tccagttcaa tgacaacctg gcggcggcgc
     2341 acattcattt tgctcatgta atactcctcc atggcagtca tcggcgagac gctggaatcc
     2401 tggagatatt ccatgacgtg gaaattttcg atggatttgg tgaatttccg gttttcgttt
     2461 tcgagattta aaattttcat aggcacgttc ctttctttca ttaagaagaa aaatagtgat
     2521 ccgtcttgtt tcttttatta tacaggaaag aaagcaaaga gcacagaaaa atttagaaaa
     2581 aaaggaaatc tggaaatcat gttgtccatt tttcagcgga gtgataagat aaagttcaga
     2641 aaacgcaagc agtacggtca gaggaggaat ttattatgag tacaattact gtcagagatg
     2701 caaaaataga ggatgccgca cgcattctgg agatttatgc ttattatgta gaatacaccg
     2761 tcatttcttt cgagtgggac gtgccgtctc tggaagaatt tgagaaccgc atgcgtgata
     2821 tcatgaaaaa atatccatac ctcgtcatcg agcaggatgg aaaaatcgaa ggttatgcat
     2881 acgcacatgc ctttgtcggc cgcgccgcat acgactggtc gagcgagctg accatttatc
     2941 tcgatcccaa tgcaagaaaa ggaggccttg gccggagact gtatgaagag ctggcggacc
     3001 gcctgaaaaa aatgggcatt ctgaacctct acgcctgcat cggctatccg caggtcgagg
     3061 atgaatatct gacgaaaaac agcgccgagt tccatgcgca tctcggattc gaactcgtgg
     3121 gaaccttcca caactgcggc tacaaattcg accgctggta cgatatggtc tggatggaaa
     3181 aaatcatcgg cgagccgcac gcgggacagc cggcagtaaa gccgtacagc gaggttaaag
     3241 aatgagtacg ataacgacgt atacaagaaa acacttcgat cccgtacatc ctgatccggg
     3301 gctgctctgt gtggaggatt ttgcgcatgc gctgccgatg atctgccgcg gaaacggcca
     3361 tgtgacgacg ttctggtcag tgggagagca ctgcatctgc tgtgcgaaag aggcggccgc
     3421 gcgcggctat tcccggcggc tggtgctggc gtgccttctg catgacgcga gcgagtgtta
     3481 catgtcggac gtgccgcggc cattaaaaca ggaaatgccg cggtaccgcg agatcgagga
     3541 ccatctgctt tcggtgattt atgagaaata tcttggcagt gatctgacgg cggaggagca
     3601 gcggatgatg aaacagatcg acaatgatct gctgtggtac gat
//
LOCUS       DAOMUF010000196         3632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7233_length_3632_cov_3.601901, whole genome shotgun sequence.
ACCESSION   DAOMUF010000196 DAOMUF010000000
VERSION     DAOMUF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7233_length_3632_cov_3.601901"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLN77_09325"
     CDS             complement(<1..757)
                     /locus_tag="PLN77_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM44503.1"
                     /translation="MSFHIVADSCCELTADMKKRGNIEIAPLTLEVGGESILDDETFD
                     QKSFLRKVAECPECPKSACPSPEYFRTAFLNGAKHCYAVTLSAQLSGSYNSAVLGANL
                     AQEEDEDLKIHVFNSRSASIGETLIVKKIVECEEAGMSFERVVETVELYISTQHTYFV
                     LENLETLRKNGRLSKTKALVASALKIKPVMGATPEGDIVQLDQARGINKALMKMVDAV
                     VNDAQHVETKTLAVSHCNCPERAEMVKEALLERL"
     gene            1084..1965
                     /locus_tag="PLN77_09330"
     CDS             1084..1965
                     /locus_tag="PLN77_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRM44504.1"
                     /translation="MEWTFSDSVRLSLLSFSEASKACAALPGFSLFRRSLQPERIEYC
                     RAELLPDCLVGSLAVPDKTHPLHLAFSLAFYLDRGQLILAADEQTLPALQSLLFPEEP
                     LDTSDVFHLFFSFLESLIQEDMLYLQNFEKRLATIEESLLDTLPKELQRTLITIRREL
                     LVFHAYYQQLLDMCEILSEDENHFLPPECARQFSRLGNRVDRLYDHTQMLREYALQIR
                     EMQQAQIDLRQNDTMRILTVVSTIFFPLSLIAGWYGMNFTHMPELSAPYAYFILIGVC
                     ILIVAVEIWYFKKKGWF"
     gene            1972..2478
                     /locus_tag="PLN77_09335"
     CDS             1972..2478
                     /locus_tag="PLN77_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5721 family protein"
                     /protein_id="HRM44505.1"
                     /translation="MIILQITQLKNFMNTLLRLETFDRFPVSEASITTFTTFSIDGSL
                     HTDFFDPDDAQLLKEKGRTRLLWKDVKSFCYSVIKGKRTPLQFKFVFQLPQAACEKMI
                     ADHALPLEIENVFGCFLNFQYKDGKLLLTTGTSLKIFTMDKSLDRVFDEEVRQFLLKN
                     EIAFEEQI"
     gene            2513..3598
                     /locus_tag="PLN77_09340"
     CDS             2513..3598
                     /locus_tag="PLN77_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HRM44506.1"
                     /translation="MNQIIMWIMAVGAVLGGVDRIAGNRFGLGKRFEEGFTLLGPTAL
                     SMSGIICLTPLLSRFLRFALVPIWNFLGLDAGLLAGILAIDMGGYQLAGELSASQEMV
                     RYAGLVIAATLGCTITFTIPVGMGMLKSGDRLFFSRGMLIGTGTLPVTMIVGGLLSGL
                     SFLQIVLQSLPVLLFCFLLMFGIWRFPEQTVRAFTVFADVIRLLTTIGLIAGAFCYMT
                     GFSLLPDLAPLEDAMAVVSSIGIVLLGSLPTAELLQRVLKKPLSFIGRKTGMNDSSAA
                     GLLMGIVSPVPAITMMEKMDERGKIVNAAFLVSAASTIAAHMGFTFGTDPDFVVPLLV
                     AKLAGGIAAVCAALFFTKKSAYSKTRK"
BASE COUNT          743 a          988 c          888 g         1013 t
ORIGIN      
        1 caaggcgctc cagaagggct tcttttacca tctcggcgcg ctccgggcag ttgcagtggg
       61 aaacggcgag ggtctttgtc tctacatgct gtgcgtcatt cacaacggca tcaaccatct
      121 tcatcagcgc tttgttgatg ccgcgcgcct ggtcgagctg aacgatatcg ccctccggtg
      181 tggctcccat aaccggtttg atcttaagcg cggatgcaac aagtgctttt gttttggaaa
      241 ggcggccgtt tttccgcaac gtttcgagat tttccagcac aaaataggta tgctgtgtgc
      301 tgatgtagag ttctactgtc tcaactacac gttcaaagct cataccggct tcttcacact
      361 ccacgatttt tttcacaatc agtgtctcac cgatggatgc acttctggaa ttaaacacat
      421 gaattttcag gtcctcatct tcttcctgtg ccaggttggc tccaagcact gcactgttat
      481 aggatccgct gagctgtgcg gaaagcgtta ccgcatagca gtgctttgcc ccgttcagaa
      541 atgccgtgcg gaaatactcc ggagacgggc atgcggattt tgggcattcc gggcactccg
      601 ccacttttct taggaatgac ttctgatcaa atgtttcgtc atccagaatg gattcgccgc
      661 cgacttccag ggtcagcggt gcaatttcaa tatttcctct ttttttcata tctgctgtca
      721 gttcacagca gctgtctgca acaatgtgaa aactcatata ttttgtccct ccaaacttga
      781 ttccgctttt tctcactgcg gacgtttcgt atttccaatc accgctcttt ttcaaacctt
      841 cctgaactag ggacgaattc gacacagtga attttttgat attttgtcat gtagttttga
      901 taactacatt ttatcataac acacttagtg ctccattgca aaaagttttt tcccaataaa
      961 agtttttccg ttaaaagttt tttccttttc ttgtcgcact cccctttttg cggtatacta
     1021 cggtcatacg gcttttttgt ttatggatat ttttccattg cttattttca ggaggtggta
     1081 cgtatggaat ggactttttc ggattcggtc cggctttccc tgctttcgtt ttcggaagct
     1141 tccaaagcct gcgcggcgct gcccggattt tctcttttcc ggcgcagtct gcagccggag
     1201 cgcatcgaat actgccgcgc agagctgctg cctgactgcc tggtcggatc gcttgccgtc
     1261 ccggacaaga cgcatccgct tcaccttgca ttctcgcttg cgttttatct cgaccggggg
     1321 cagcttattc tggcagccga cgaacagaca cttccggctt tgcagagcct cctcttcccg
     1381 gaagaaccgc tcgatacttc cgatgtcttc cacctgtttt tctcgtttct ggaatcgctg
     1441 atccaggagg atatgctcta tctgcagaat tttgagaagc gcctcgcgac aatcgaggag
     1501 tctcttctcg acactctgcc aaaagagctg cagcggaccc tgatcacgat ccgtcgggag
     1561 ctgctcgtgt tccatgccta ttaccagcag cttctcgaca tgtgcgaaat actctccgag
     1621 gacgaaaatc actttcttcc cccggaatgc gcccgtcagt tttcgcgcct tggaaaccgc
     1681 gtggaccgtt tgtacgacca cacccagatg ctgcgggagt acgccctgca gatccgcgag
     1741 atgcagcagg cgcagatcga cctgcgtcag aacgatacga tgcggattct gacggtcgtc
     1801 tcgacgatct ttttcccgct ttccctgatc gccggctggt acggcatgaa ttttacgcat
     1861 atgcctgagc tttctgcccc gtatgcgtac ttcatcctga tcggcgtctg tattctcatt
     1921 gttgctgttg aaatttggta ttttaaaaag aaaggctggt tttaactacg gatgattatc
     1981 ctgcagatta cacagttaaa gaattttatg aacaccctgc tccgtctcga gacatttgac
     2041 cgctttcccg tctccgaggc gtcgatcaca acgtttacga cgttctccat cgacggcagt
     2101 cttcacacgg actttttcga tcccgatgac gcgcagctct taaaagaaaa agggcggacg
     2161 cggcttctct ggaaagatgt gaaatcgttc tgctattccg tcatcaaagg aaaacggaca
     2221 cccctgcagt tcaaatttgt gtttcagctt ccgcaggcgg cgtgcgaaaa aatgatcgcg
     2281 gaccatgcgc tgccgctgga aatagagaac gtgtttggat gctttctgaa ttttcagtat
     2341 aaggacggaa aactcttact taccaccgga acttccctca aaatctttac gatggacaag
     2401 tcgctcgacc gcgtcttcga tgaggaagtc cgccagtttc tgctgaaaaa tgaaattgca
     2461 tttgaagaac agatataaag acaaaatact tatttagaaa ggttctcttt ttatgaatca
     2521 gattattatg tggatcatgg cagtcggcgc ggtactcggc ggtgtcgacc ggatcgctgg
     2581 caatcgcttc ggacttggaa aacggtttga ggagggcttt acgctgcttg gaccgacggc
     2641 actttccatg tcgggcatca tctgcctcac cccgctgctc tcccgctttc tccgtttcgc
     2701 cctcgttccc atctggaact ttttggggct ggatgcggga cttctcgccg gcattctcgc
     2761 cattgacatg ggcggctacc agctcgccgg agagctttcg gcatcgcagg agatggtgcg
     2821 ctacgcagga cttgtgattg cggcgacgct tggctgtacc atcaccttta cgattccggt
     2881 cggcatggga atgctgaaaa gcggcgaccg cctctttttc tcccgcggca tgctgatcgg
     2941 cacgggcacg cttcccgtca cgatgatcgt cggcggactg ctctcagggc tctcttttct
     3001 gcagatcgta cttcagtcgc tcccggtgct cctcttctgc tttctgctga tgttcggcat
     3061 ctggcgtttt ccggaacaga ccgtgcgcgc gttcacggta tttgcagatg tgatccggct
     3121 tctgacgacg atcggcctca tcgcgggcgc gttctgctat atgaccggct tttcgctgct
     3181 cccggacctt gcgccgctgg aggacgcgat ggcggtcgtc tcgtccatcg gcattgtact
     3241 gctcggcagc cttccgacgg cggagctttt acagcgggtg ctcaaaaagc cgctctcttt
     3301 tattggaaga aaaaccggca tgaacgacag cagtgccgcc ggccttctga tgggcattgt
     3361 cagccctgtt ccggcgatca caatgatgga aaaaatggac gagcgcggaa agattgtaaa
     3421 cgcagctttc ctcgtctccg ccgcatcgac catcgccgca catatgggat ttacgttcgg
     3481 caccgatcct gattttgtcg tgccgcttct cgttgcaaag ctcgccggag gcatcgccgc
     3541 cgtctgtgcc gcactgtttt tcacgaagaa atctgcttat agcaagacca gaaaataacg
     3601 caaaaaaggc agtgctgcag ggcgcgaatt ac
//
LOCUS       DAOMUF010000197         3605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7306_length_3605_cov_2.636620, whole genome shotgun sequence.
ACCESSION   DAOMUF010000197 DAOMUF010000000
VERSION     DAOMUF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7306_length_3605_cov_2.636620"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLN77_09345"
     CDS             <1..674
                     /locus_tag="PLN77_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM44507.1"
                     /translation="EDSDVLVVNKPKDMVVHPAAGHYSHTLVNAVLYHCRGQLSGING
                     VLRPGIVHRIDMDTTGALVICKSDFAHQSLAEQLSVHSITRKYRAIVHGNLKEDEGTV
                     RGAIGRHPTDRKKMAINERNGKPAVTHYRVLERFGNYTYIECQLETGRTHQIRVHMAS
                     IGHPLLGDAVYGPKKCPVKNLQGQTLHAMVLGFIHPRTGAYMEFEAPLPEYFSNLLLQ
                     FRKNV"
     gene            848..1486
                     /locus_tag="PLN77_09350"
     CDS             848..1486
                     /locus_tag="PLN77_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRM44508.1"
                     /translation="MKTIITIGRQYGSAGHSIGKILADDLGIKYYDKELLERAAKDSG
                     LCQELFENHDEKPTNSFLYSLVMDSYSFGYGSTMMDMPLNQKVFLAQFDAIKKISQDG
                     PCVIVGRCADYALEENPNVLSIFIKADMQDRIRRIAKLYDLSDAKAKDKITKIDKQRA
                     SYYNYYTSKRWGEVDSYDLCLNSSVFGIDGTVDMIKQAIEVKEAGIRKIFSI"
     gene            1562..2002
                     /gene="dtd"
                     /locus_tag="PLN77_09355"
     CDS             1562..2002
                     /gene="dtd"
                     /locus_tag="PLN77_09355"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRM44509.1"
                     /translation="MRIVIQRVKRASVSVEEKVVGSIQKGFLVLIGVGQGDDEATADK
                     YMKKLLGLRIFEDENGKTNKSLADVDGQLLMVSQFTLYANCKKGNRPSFTEAGEPHRA
                     EELYEYMVAKAREQVPVVETGVFGAEMEVSLVNDGPFTVILEDL"
     gene            2169..3116
                     /locus_tag="PLN77_09360"
     CDS             2169..3116
                     /locus_tag="PLN77_09360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM44510.1"
                     /translation="MPQFDYQQLCHFVLPEQGGLSVETILKRFLHLTPREIRHAKYIP
                     DGIQKNGVSCRTNAVVQTGDTITFRYEQKCPHLSEDQVLPVSGDSAGVRILYEDSDLC
                     VADKPSGMVCHASHGHYSDSLTHAAASLLKTPTAPSELRCIGRLDKDTSGLVLFAKNQ
                     LAAARLSRQRESGALKKEYLALVHHPFSEETQKETIRLSMEQIPGDDPDSFDPSGKRI
                     TKMRICKEGNGLWATTHYEAVLQDPFWAVVGCRLETGRTHQIRLHMASIGHPLFGDPL
                     YGVRDGGTRAALHAWKLHFLQPMTGEPIDLENIRTHMFD"
     gene            3146..>3605
                     /gene="mraW"
                     /locus_tag="PLN77_09365"
     CDS             3146..>3605
                     /gene="mraW"
                     /locus_tag="PLN77_09365"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HRM44511.1"
                     /translation="MEEQKEHKRRVRYKGTHPRNYNEKYKELNPEKYQGTIEHVIQKG
                     STPAGMHIPILVDEILDFLKIEPGQNGLDATLGYGGHSSRMLACLQGKGHLCALDVDP
                     IEMAKTKERLAAQGYGPEILTILHQNFANIDQVAAEYGPFDFILADLGVSS"
BASE COUNT         1027 a          807 c          914 g          857 t
ORIGIN      
        1 atgaggacag cgacgttttg gttgtcaaca agccaaagga tatggtcgtc catccggcag
       61 ccgggcatta cagccacacc ctcgtcaacg ccgtactgta tcactgtagg ggacagcttt
      121 ccggcatcaa cggcgtgctg cgccccggaa tcgtccacag aatcgatatg gacacaacgg
      181 gtgccctggt tatctgtaag agcgatttcg cccaccagag cctcgcagaa cagctctctg
      241 tacattccat tacccgaaag taccgggcaa ttgtccatgg gaatctgaaa gaggacgagg
      301 gaaccgtgcg cggtgcaatc ggccgccatc cgacagaccg gaaaaagatg gcgattaatg
      361 aacggaacgg aaaaccggcg gtcacacatt accgtgtgct ggaacgcttt ggaaattata
      421 cgtatattga atgccagctg gaaaccggcc ggacgcatca gattcgtgtc catatggcaa
      481 gtatcgggca tccgctgctt ggcgatgcgg tttatggccc gaagaaatgc ccggtgaaaa
      541 acctgcaggg acagacgctg catgcgatgg tacttggatt tatccatcca cggaccggtg
      601 cctatatgga gtttgaggcg ccgcttccgg aatatttttc caatctgttg ctgcagtttc
      661 gaaaaaatgt ctgaaactgc ttaaaaagaa tgatatttca aaaagtatat agttaatttg
      721 tataagtttt ttcaaaatgt tctgtacttt ctgtgaattc tgtagtatac tagatgtaac
      781 gaaaaaacgc gagttactac atctgtgaaa gcagattatt gtgacggaat ggaaaggagc
      841 ggattatatg aaaactatca taacgattgg acgtcagtac ggaagcgccg gacattccat
      901 tggtaagatt ttagcggatg acctgggaat caagtactat gacaaagagc tgctggagag
      961 agcagcaaag gacagcggat tatgccagga attgttcgaa aaccacgatg aaaaaccaac
     1021 caacagcttt ttatactctc tggttatgga cagttactcc ttcggctatg gatctaccat
     1081 gatggatatg ccgctgaacc agaaggtatt tctggcgcag tttgatgcca tcaagaagat
     1141 ttcgcaggac ggtccgtgtg tcatcgtagg ccgctgtgca gattatgcac tggaggaaaa
     1201 tccgaacgtg ctgagcatct ttattaaagc agatatgcag gatcgcatcc gccgtatcgc
     1261 aaagctgtat gatctttctg atgcaaaagc aaaagacaag atcacaaaga tcgacaaaca
     1321 gcgtgcaagc tactacaatt actacaccag caaacgctgg ggcgaggtag acagctacga
     1381 cctgtgcctg aacagcagtg tattcggtat cgatggaacc gtagatatga tcaaacaggc
     1441 aattgaagtt aaagaagcgg gaatccgcaa gattttcagc atctgatcaa aacagaacat
     1501 aggagaaacg accctcctct taaaaataag aggagggtca ttttatgaaa ggattttaaa
     1561 gatgagaatt gttatacaac gtgtaaaaag agcaagtgtt tcggtggaag agaaggttgt
     1621 tggatcgatt cagaaaggtt ttctggttct gatcggtgtc ggccaggggg acgatgaagc
     1681 aacagcagac aagtatatga aaaaactgct gggactgcgt atttttgaag atgaaaatgg
     1741 aaagacgaac aagtccctgg cagatgtgga tggacagctt ctgatggttt cacagtttac
     1801 cctgtatgcc aactgtaaaa aaggaaaccg tccaagcttc acggaagcag gcgagccgca
     1861 cagagcagaa gaactgtatg aatatatggt cgcgaaagca cgggaacagg ttcctgttgt
     1921 cgaaacggga gtgttcgggg ctgagatgga agtatcactg gtgaatgatg ggccgtttac
     1981 ggtgatctta gaggatctgt aaataaaatt tttttgtttg ccgaatcggt aaaacagaac
     2041 gtggtttgcg agatctgtga aacctgtttt tgtctagaga tagtgtacca tcatcgtact
     2101 gaattgtttg tatttatata gtttacataa aatgattatg tgaacttgtg aagaggagaa
     2161 aatcttttat gccgcaattt gactatcagc agctgtgtca ctttgtcctt ccggaacagg
     2221 ggggactgtc ggtggaaaca attctaaaac gttttctgca cctgacacca cgtgaaatcc
     2281 gccatgccaa atatattccg gatggaattc agaaaaacgg tgtttcctgc cgcacaaatg
     2341 cagtcgtcca gactggagat acgatcactt ttcgatatga gcagaagtgt ccccaccttt
     2401 ccgaagatca ggttctgccg gtatcaggtg attctgccgg cgtacggatt ctctatgagg
     2461 acagtgatct ctgtgtcgca gacaagccat ccggtatggt ctgccacgct tcccacggcc
     2521 attattccga ttccctgaca catgctgccg catccctgtt aaaaacaccg acagcgcctt
     2581 ctgaactccg ctgcatcggc cgtcttgaca aagatacatc gggtctggta ctatttgcaa
     2641 agaatcagct ggctgctgcc agactgtccc ggcagcggga gagcggcgcg ctgaaaaaag
     2701 aatacctggc tctggtacac catccctttt ccgaagaaac gcaaaaagaa accattcgtc
     2761 tttccatgga gcaaattcca ggagatgacc ctgattcttt cgatccgtct gggaaacgta
     2821 tcactaaaat gcgtatctgt aaagaaggga acggcctgtg ggctactaca cactacgagg
     2881 ctgtccttca ggaccctttc tgggccgtcg tgggctgccg tctggaaacc ggccgcacgc
     2941 atcagatacg ccttcacatg gcatctattg gacatccact tttcggagat ccgctctacg
     3001 gagtccgcga cggcggtacc cgtgccgcgc tccatgcctg gaaactgcac tttctgcagc
     3061 cgatgacagg agaaccaatt gatctggaaa atattcgaac gcacatgttc gattaaatat
     3121 atttgaagta taaggagaac attttatgga agaacagaaa gaacacaaac gccgcgtccg
     3181 ctacaaaggc acacatccgc ggaattacaa tgagaaatac aaagaactga atccggagaa
     3241 atatcagggg accattgagc atgtgatcca aaagggaagc acaccggccg gcatgcatat
     3301 cccgattctc gtagatgaaa tcctcgattt tctcaagatc gagccgggac agaacggact
     3361 tgatgcaaca ctcggatacg gcggccacag ctcccgtatg cttgcatgcc tgcagggaaa
     3421 agggcatctc tgtgcgctgg atgtcgatcc gatcgagatg gcaaaaacaa aagaacgact
     3481 ggccgcgcag gggtacggcc ctgagatttt aacgattctt catcagaatt ttgccaatat
     3541 tgatcaggtt gctgccgaat acggcccctt tgacttcatt ctggcggatc ttggtgtttc
     3601 ctcca
//
LOCUS       DAOMUF010000198         3561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7421_length_3561_cov_4.181118, whole genome shotgun sequence.
ACCESSION   DAOMUF010000198 DAOMUF010000000
VERSION     DAOMUF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7421_length_3561_cov_4.181118"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLN77_09370"
     CDS             <1..260
                     /locus_tag="PLN77_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM44512.1"
                     /translation="KGKRIRGNTFNKRLDLVLKAVGLHHRSIHKGRKTYGTTLIDAGC
                     EDSLVMNQLGHTTIETSRKYYYFSNRTREHQIEEIKKAVNI"
     gene            617..1216
                     /locus_tag="PLN77_09375"
     CDS             617..1216
                     /locus_tag="PLN77_09375"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRM44513.1"
                     /translation="MSREKNGSRGTVKAVIYLLIVVILAGMVVYLTQSLRRDREPEIT
                     GTFIDSKLEAAGELTSAKMIYNGLIHYSDGSIPFLTQKAFNMTYRAEVRAGVDLSKAN
                     TEVTDSEVTVTLPAVEIFDISIDNDSIQYYDEKAALLNWERKEDAMDAIASAKEDVEQ
                     QTKEMDDLETMAQEQAKTLITGMLSETVGDKTLVVKFEE"
     gene            1607..2368
                     /locus_tag="PLN77_09380"
     CDS             1607..2368
                     /locus_tag="PLN77_09380"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM44514.1"
                     /translation="MDYKLLLDTAVMAGEILLESGAETWRVEDTMLRMLRMSGLKTAD
                     VLALTTGFVVTLDDPAMDSMTVMRSVESRATDLNRIHAVNQLSRDFCEGNIDLDALFH
                     RVRDIYRAKSEIKKNLLAMMATTAGFAIMFGAGFYEVLASALAGFISGIGVYGGKKAG
                     MQSFLVNCIGSFLLAVTSILCVHYLPGTMDLNVIIIASIMPLVPGVAITNAVYDTLHG
                     DYISGAARMLEAFVTAAAIAIGVGLGMLLLRPLVR"
     gene            2410..2856
                     /locus_tag="PLN77_09385"
     CDS             2410..2856
                     /locus_tag="PLN77_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM44515.1"
                     /translation="MRIAGEIILKSLGAWVAIWGFSRLLNAPKKLECYTAGVGMIGWA
                     VYLIVEVFNDGILMKNFLAAVAVALVSQAMARHFKAPVILFVIPGILPMVPGAGMYRI
                     VYSIVMGPENMVGTYLLETATAAGAIALGIFLGEALQKVFFYEFHR"
     gene            3215..>3561
                     /locus_tag="PLN77_09390"
     CDS             3215..>3561
                     /locus_tag="PLN77_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44516.1"
                     /translation="MKFEDDDLKDGKLWAALQKEIDRDLAEKESGETFQEELADIEDS
                     IEFESKETKSEDHIPDETANLSNLRSVLEATERAKDEEAKQKMKQTKVEPVTQDIEIP
                     EFTEEEEEIEETPA"
BASE COUNT         1080 a          649 c          982 g          850 t
ORIGIN      
        1 aaaaaggaaa acgaatccgg ggaaacacat ttaataaaag attggatctt gtattgaagg
       61 cggtggggct gcatcacaga tccattcaca aaggaagaaa gacgtatgga acaacattga
      121 ttgatgctgg atgtgaagat tctttagtca tgaatcagtt gggacatacg acgatagaaa
      181 cgagtagaaa atattattac ttttcaaacc gaacaaggga acatcaaata gaagagataa
      241 aaaaggctgt aaatatatag ttttggggaa aaaggtaatc ataaggtaat caagaaaact
      301 gaatatagag tcactgttga atacattaaa aaagctgatg gttacgcggt tttttagtta
      361 taggggcatt gagaagaatg aaaaaaaacc ggattcgatt ctctcatccc ctgtattagg
      421 aaagagtgaa gatacaaaat cttcactctt ttttcgtatt gtttaaaaga aaagtctaac
      481 agaacaggat ttacagaatt ttaagatatt tttgtaaagc aggactgttt tttatggtgt
      541 aaataaggta caatagagcc acagaacata ttcaaaaata atgaaaaatg aaacatgaga
      601 agggagtggg tgtaacgtga gcagagaaaa gaatggaagc cgcggcacgg taaaagctgt
      661 catttatctt ctgatcgtag taatccttgc tggaatggtt gtttatctga cgcagtcgtt
      721 gcggcgggac cgggagccgg agattacggg gacgtttatt gacagcaagc tggaggcggc
      781 gggggagctg acgtcggcga agatgattta taacgggctg attcattact cggacgggtc
      841 gattccgttt ctgacgcaga aggcgtttaa tatgacatac cgtgcagagg tacgtgcggg
      901 cgtggatctc tcgaaggcga atacggaggt gacggattca gaagtaacag ttacacttcc
      961 ggcagtggag atttttgata tttccatcga caatgattcc atccagtatt acgatgagaa
     1021 ggcagcgctt ctgaactggg agcggaagga agatgcgatg gatgcaatcg cgagtgcgaa
     1081 ggaggatgta gagcagcaga cgaaagaaat ggatgacctg gaaacgatgg cacaggaaca
     1141 ggcgaaaaca ctgattaccg gaatgctgtc cgagacggtc ggggacaaga cattggttgt
     1201 aaaatttgaa gaatagtaca tagacgggat gtcggcactt ctttttgggg gataaaaaaa
     1261 ggagggccgg tatttcttct ggcagaaaca ccggcattat gaaaaagaaa aatctatata
     1321 aattggaaag aatattaggt tgggacaacg gttgaaagat agaggaagat gatttgttgt
     1381 ccctttgttt tgatacgttt agtatagttg tcatatgtga ggaaagtgtg aatggagttt
     1441 gaagaaactt tgaaaagatt tgtgaatggc gcgggtgaac atcggaacat cccaacgccc
     1501 ggatatcgcc gaatttgcat agaattaact tgaaagtgaa cactgcttca tggtacaatc
     1561 gtttggcatg tgtgaaatat gagttacatt tgagaggaca aaaactatgg actataaact
     1621 gctgctggac accgcggtca tggcgggaga aatcctgctg gaaagcgggg cggagacgtg
     1681 gcgggtggag gataccatgc tgcggatgct gcgcatgtcc ggcctgaaaa ctgcggatgt
     1741 actggcgctg acgaccggct ttgtggtaac gctcgacgac ccggcgatgg attccatgac
     1801 tgttatgcgc agcgtagaga gccgtgcaac ggatctgaac cggatccatg cggtcaatca
     1861 gctttcccgc gatttctgcg aaggaaatat cgacctggat gcgctgttcc accgggtgcg
     1921 ggatatctat cgtgccaagt cggaaattaa aaaaaatctg ctcgcgatga tggcgacgac
     1981 cgcaggcttt gcaatcatgt tcggagccgg gttctacgaa gtgctggcat ctgcacttgc
     2041 aggttttata tcaggaatcg gtgtttacgg aggaaaaaaa gccgggatgc agtcgtttct
     2101 tgtgaactgt atcgggtcat ttttgctggc ggtcacatct attctctgtg ttcattacct
     2161 gccggggacc atggatctga acgtcattat cattgcatcg atcatgcccc tcgtaccggg
     2221 tgtggcgatc acgaatgcgg tgtatgatac cctgcacggc gactacattt ccggtgcggc
     2281 acggatgctt gaggcgtttg taaccgctgc ggccatcgca atcggcgtgg gacttggcat
     2341 gctgctgctc cgtccgctgg tgcggtagag aaaaccgata cagaaaaaag atgaggttaa
     2401 agcaaaaata tgagaatagc aggtgaaatc atcctgaaat cccttggagc ctgggttgca
     2461 atctggggat tttcaagact gttgaacgca ccaaaaaagc tggaatgcta cacggccggt
     2521 gtcggtatga tcggctgggc ggtctatctg atcgtggaag tattcaacga cggcattctg
     2581 atgaagaact ttcttgccgc cgttgccgtc gccctggttt cccaggcaat ggcgcgtcat
     2641 ttcaaagcgc cggtgatcct tttcgtgatc ccgggcattc ttccgatggt gccgggagcc
     2701 ggtatgtacc gcatcgtcta ctcgatcgtt atggggccgg aaaacatggt tggaacgtat
     2761 cttttagaga cagcgaccgc ggcaggcgca attgcccttg gaattttcct cggagaagcg
     2821 ctgcagaagg tattttttta cgaattccat aggtgattta cgaaaaaagt gagaaatttg
     2881 ccaaaaccaa attttttgct tttttttgtc gaattttatg atatacttaa cagtgtatgt
     2941 gcccttggac aaaagaagaa atctataggg gcatttttaa catcagtaga caagaagtcc
     3001 cccgcttcta aagcggtggg atgaattgtt aaaagaaaca taacggcagt ggcgggcgga
     3061 gagaaatcgg tatcctccac tgacatgagg caagtcacag tcgaactatt ttcgacatgt
     3121 cagactccgt gaaaataccg actgtgagtt gaaacaagga aaaaagaaga gaagtaagta
     3181 aagtgcagac acagaactat gaggagcaaa aaatatgaag tttgaagatg atgatttaaa
     3241 ggacggtaaa ctttgggctg ccctgcaaaa agagattgac cgtgacctgg cggaaaagga
     3301 gagcggtgaa acattccagg aagaactggc agacatcgaa gactccattg aatttgaaag
     3361 caaagaaaca aaaagcgaag atcacatccc ggatgagact gcaaatttaa gtaatctgcg
     3421 aagtgtcctt gaggcaactg agcgtgcaaa agacgaagaa gcgaagcaga aaatgaagca
     3481 gaccaaagtg gagcctgtga cgcaggacat cgagattccg gaatttacag aagaggaaga
     3541 agaaatcgaa gagacgccgg c
//
LOCUS       DAOMUF010000199         3558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7431_length_3558_cov_3.945761, whole genome shotgun sequence.
ACCESSION   DAOMUF010000199 DAOMUF010000000
VERSION     DAOMUF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7431_length_3558_cov_3.945761"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..2080
                     /locus_tag="PLN77_09395"
     CDS             110..2080
                     /locus_tag="PLN77_09395"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRM44517.1"
                     /translation="MNRTQKLGNVLIISSRKRMLSEFQSYLHSVLGEYLTFNTLLREQ
                     ATDPSLFRGYQCVLFPTARAMENFPLTIDPSILQLPCDRVFNHMFLDKIIQIPPHERV
                     YLVNDDRYSTLAIISQLEECGITQYDFVPFYPGCKDTESDIQFAITAGEPQLVPSRIP
                     NVLDIGNRIIDISTILQLCEYFNIPLQTVNRVSRNYVNQILHTVKTSETYYTNYVQTE
                     QLMQVILFSLPIGICLFGADGRIQFMNAKFAHAFSLPSSNFEGQPFSECFPPAYADTA
                     FDRNGDWTILLSDQKTQITLSVLSMVFPDTEPVFLAFLPEGRSTEMSVPKDEKTEEST
                     FFASSRTFSLSLSHADSVQNLMAQARHLALFDFPILIQGESGTQRRTLARAIHNFSRR
                     HHYPFSVLHSPGNDLTEASLLRLLAETNHGTLVLSQVDRFPLSIQDLLVNVLTNVHGN
                     FFSAPETRRFDVRIIAIADDNLYKKVEKGTFLRELFHLLSASELQTVPLRRRREDIPD
                     LLNYFLLQFFHNTDMTCDRIFSEGLLRFLKEYAYPGNIHELYNLSCSLFTRYSSHKLQ
                     LSDLPTYLRSRQMADVSLTDLQYQVLSFVSTHPKAGRLAIKNGLADGETFVSEAALRT
                     VLGELSSAGYLIVHRTKGGCEISELGRMVLEK"
     gene            complement(2082..3149)
                     /locus_tag="PLN77_09400"
     CDS             complement(2082..3149)
                     /locus_tag="PLN77_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRM44518.1"
                     /translation="MRQKRISDYGYRIGHHENGPRNKITDVPGVRVGHATVESGNQHT
                     GVTVIVPGEGNPFLKKYLASAYVHNGFGKTCGLVQVQELGTLETPIALTNTLNVGKVA
                     DAMISWMIQQTKKEGEDVFSINPVVGECNDSRINEICNRVVGEKELYQALEQADTDFA
                     EGDIGAGTGTICYGLKGGIGSASRTLVLDGKTYTIGVLVQSNFGATRDLKISGKPAGE
                     KILERIRKEECGSSAEDRGSIMTVLATDLPVSERQLYRIIRRCGVGIARTGAYTGHGS
                     GEIMIGFTTANRVDTKSTCEVEACARIKEEKINEAFEAAAEAVEEAILNSMVTAKEKR
                     GLDGARYRSLAEFLEIPGFLK"
BASE COUNT          761 a         1032 c          686 g         1079 t
ORIGIN      
        1 ttttcttttt tgtctttttg catttaggtt gtttattttc gtcaaaaatg gtataattat
       61 cctaattttt aacccattac cactttttag caggaaaaga ggtcccttta tgaacagaac
      121 acaaaagctt ggcaacgtat tgatcatttc ttcccggaaa cgaatgcttt ccgaatttca
      181 gtcttatctt cactccgtgc tcggtgaata tctcaccttt aatacccttt taagagaaca
      241 ggccacagac ccatccctct tccggggata ccagtgcgtc ctttttccaa ccgcccgcgc
      301 catggaaaac tttccgctta ccatcgaccc ttccattctg cagctcccct gcgaccgcgt
      361 tttcaaccat atgttcctgg ataaaatcat tcagattccg ccgcacgaac gtgtctatct
      421 ggtaaacgat gatagatatt ccacgctcgc tatcatttcc cagcttgagg aatgcggcat
      481 cacacaatat gactttgttc cgttttatcc cggatgcaag gataccgagt ctgacattca
      541 gtttgctatc accgccggcg agccgcagct cgttccttcc cgcattccga atgtgctgga
      601 tatcggcaac cgtatcatcg atatttccac tattttacag ctctgcgaat atttcaacat
      661 tcctctgcag accgtaaacc gtgtatcgcg caattatgtc aatcagattc ttcacaccgt
      721 caagacctca gaaacttact ataccaatta tgtacagacc gaacagctca tgcaggtgat
      781 cctgttctct cttcccattg gcatctgtct gtttggtgcg gatggccgca ttcagttcat
      841 gaacgctaaa tttgcacatg ccttctcgct tccctcctcc aactttgaag gacagccttt
      901 ttcggaatgt tttccgcctg cctacgcgga tacggctttc gaccgcaacg gtgactggac
      961 tattctcctt tcggatcaga aaacacagat cacgttaagc gttctctcta tggtttttcc
     1021 ggatacagag cctgtttttc ttgcgtttct tcccgagggc agatccacgg aaatgtcggt
     1081 tccgaaagac gaaaaaacgg aagaaagcac tttttttgct tcctcccgta ccttttcgct
     1141 ttccctgtcc cacgcagatt ccgtccagaa tctgatggcg caggccagac atcttgcact
     1201 ttttgatttt ccgattctga tccagggaga aagcggtaca cagcgcagga cactcgcacg
     1261 tgcgatccac aatttttcgc gccgccatca ttatccgttt tccgttcttc attctcccgg
     1321 aaacgatctg accgaagctt ctctgctccg gcttcttgcc gaaaccaacc atggcacact
     1381 cgttctttcc caagtggacc gttttccgct ttccattcag gatctgctgg tcaatgttct
     1441 taccaatgtt catggtaact tcttttccgc accggaaacc cgccgtttcg atgtgcggat
     1501 cattgctatt gcggatgata acctctacaa aaaagtagaa aaaggaacct tcctgcggga
     1561 actttttcac cttttaagtg cttcagagct tcagactgtc ccgctccgca gacgcaggga
     1621 ggatattccc gatcttctca attactttct gcttcagttt ttccacaaca ccgatatgac
     1681 ctgcgaccgg atcttttcgg aagggcttct gcgctttctg aaggaatatg cgtaccccgg
     1741 aaatattcat gagctgtaca atctgtcctg cagcctgttt acccggtaca gcagccataa
     1801 gctgcagctt tccgatctgc caacgtacct ccgctcccgc cagatggctg acgtttccct
     1861 caccgacctt caatatcagg ttctttcttt cgtatcgacg catccgaaag caggccgcct
     1921 ggcaatcaaa aacggactgg ccgacggaga aacctttgta tccgaagcgg ctctgcgcac
     1981 cgtgctcgga gagctttctt ccgccggtta tctcatcgtg caccggacaa aaggcggatg
     2041 tgaaatttcc gagctggggc gtatggttct ggaaaaataa tttatttcag aaatcccggt
     2101 atttccagaa attctgcaag gctgcggtat ctggctccgt ccaggcctct tttttccttt
     2161 gcggttacca tagagttcag gatcgcttcc tccacagcct ctgccgctgc ttcaaatgcc
     2221 tcgttgattt tttcttcttt tatccgggcg cacgcttcca cctcacatgt actctttgtg
     2281 tctacgcggt ttgctgttgt aaatccgatc atgatttcac cgcttccgtg gccggtgtag
     2341 gctcctgttc tggcgattcc gactccacag cggcggataa tccggtacag ctgccgctcc
     2401 gataccggaa ggtctgttgc cagaaccgtc ataattgatc cccggtcttc ggcgctgctt
     2461 ccgcattctt ccttccggat ccgctccaga atcttctctc ctgccggttt tccactgatt
     2521 ttcaaatctc tggttgcccc gaaatttgac tgtaccagaa caccaatggt atatgttttt
     2581 ccgtcaagca ccagcgtgcg ggatgccgat ccgattcccc ctttcagccc atagcagata
     2641 gttcctgttc cggcaccgat atcaccttcc gcaaagtctg tatccgcctg ctccagtgcc
     2701 tgatacagct ctttttcccc cactacccgg ttacaaatct cattgattct cgaatcatta
     2761 cattctccga caaccggatt gatggaaaaa acatcttccc cttctttttt ggtctgctgt
     2821 atcatccatg aaatcatggc atccgccact tttccgacat tcagtgtatt ggtcagggca
     2881 atgggcgttt caagcgttcc gagctcctgt acctgcacaa gaccgcacgt tttcccgaaa
     2941 ccgttatgca cgtaggcaga cgccaggtac tttttcagaa acggattgcc ttctcccggc
     3001 acaatcaccg tcactccggt atgctgattt ccactttcca ccgtcgcatg gcctacccgc
     3061 acaccgggca catccgtaat tttgtttcgc ggtccgtttt catgatgtcc gatccgatat
     3121 ccgtaatcgc tgattctctt ctgtctcatc ctgtttcctc ctctttttat gatgtactcc
     3181 cggaattttt cagctcacgc aaatacaggc acaaaaaaga ctccgaagat acatttttct
     3241 ctcactttac gctctcttct ctcttctgtc catacctctt cccacaattt ttcttctttt
     3301 ctttatattt tttccatttc ttttagaatt tggacacact ttgctcacat attcctttta
     3361 taataaagct cggatagttg ataaaccact cactatctcc cccctcataa aaagatggcg
     3421 atatactttt gtatatcgtc atctttgttt ttgctgacaa aaacgccccc gggtggaaaa
     3481 cgtaaccact tccgttttcc gcccgggggc attctttctt tttgctcagg cctctgcagc
     3541 agcttcctct gcttccgc
//
LOCUS       DAOMUF010000200         3542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7482_length_3542_cov_3.999426, whole genome shotgun sequence.
ACCESSION   DAOMUF010000200 DAOMUF010000000
VERSION     DAOMUF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7482_length_3542_cov_3.999426"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="coaBC"
                     /locus_tag="PLN77_09405"
     CDS             complement(<1..747)
                     /gene="coaBC"
                     /locus_tag="PLN77_09405"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRM44519.1"
                     /translation="MENLDGKCVVLGVTGGIAAYKMANVASALRKAGADVHVIMTENA
                     TQFITPLTFETLTNNRCVVDTFARDFKYEVTHISLAKAADLIMIAPATANVIAKMAHG
                     IADDMLTTVVLAAKCKKLVAPAMNTAMLENPITQDNLATLKKYGFGIIEPTVGMLACK
                     DVGKGKLPDPEVLLDYIAMELAREKTLAGVRVTVTAGPTQESLDPVRYLTNHSSGRMG
                     YAIAREAMLRGAQVTLISGPVSLAPVPGVAM"
     gene            914..1699
                     /locus_tag="PLN77_09410"
     CDS             914..1699
                     /locus_tag="PLN77_09410"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRM44520.1"
                     /translation="MILAIDIGNTTVTLGGVRIAPQQAYSLEFTARIDINHTWSASQY
                     ESAIEQLFEDKNLSPSDFRGVIISSVVPSVLTLLQECVRNLFGTEPLLVTSESDLGMT
                     FSIPEPEKVGRDRLVDAAWAAAHYPLPVITVDLGTATTFNVIRENSVFCGGAIAPGLD
                     MGLGALSERTAQLPRLDLQIPKRIIGRNTEECILSGTIVGMAAMLDGMVQRIEAELGS
                     PATLILTGGAARFVEPLVLHPHIYDPNLLLKGLAFLCERNCAN"
     gene            complement(1790..2377)
                     /locus_tag="PLN77_09415"
     CDS             complement(1790..2377)
                     /locus_tag="PLN77_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44521.1"
                     /translation="MLAGVILCVIAGCLVKRMTAVYTQKENPIGPERGTTDVFLTAAD
                     FISEKSDQELLQEIETFDTDKAEMIYLYIPEDTGAGMSFAITREDHPEEMDEILSTIY
                     GEKISCQKPKQVFNMTERILYDYNWYVLIYDHEMNQMALVFLYSDDVIVYNGHVLQTT
                     ETYDESIRPCILKLKEENQEKLKSGEATQRRVAIP"
     gene            complement(2572..3282)
                     /locus_tag="PLN77_09420"
     CDS             complement(2572..3282)
                     /locus_tag="PLN77_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44522.1"
                     /translation="MNKKLAGIFAMCALLLTGCQGAKESSKEITPPDTGWGKTVDEVL
                     ADWNLDRAQVEIFSETESAAAIAVDTEATVFGEQTSRVMFQFINLDQTGATGKPVLCE
                     VDITYPDDADMDTVKKEMEKSYGSSKDSITRYELYQSLGDDQLPEYTYKKADQLAVWS
                     GESLKDAIPSDKSTEYETAWEAYQPGLTTDNWENYTEQASMATAVCASGAEAFPMFEK
                     NGVSLEAYPGLVYEQVKK"
BASE COUNT          798 a          990 c          758 g          996 t
ORIGIN      
        1 catcgcaacg cctggaaccg gggctaatga aaccgggccg gaaatcagcg tcacctgcgc
       61 gccgcggagc atcgcctccc gcgcaatcgc gtatcccatg cggccgctgc tatggttcgt
      121 cagataccgc accggatcga gggattcctg cgtcgggcct gcagtcacgg tgacacgcac
      181 accagcgagc gtcttttcgc gggcaagctc catcgcgata taatcgagca gcacctccgg
      241 gtccggcagt tttccttttc cgacgtcctt gcacgccagc attcccacgg tcggctcgat
      301 gatgccgaaa ccgtattttt tcagcgtcgc cagattatcc tgcgtaatcg gattttccag
      361 catcgccgta ttcatcgccg gagcgaccag ctttttgcac ttcgctgcga gcaccaccgt
      421 tgtcagcata tcatctgcaa tgccgtgtgc catttttgca atcacattgg cggtagccgg
      481 ggcaatcata atcagatccg ccgcttttgc cagcgaaata tgcgtcacct catatttaaa
      541 atcacgcgca aacgtatcga ccacgcagcg gttgttggtc agcgtctcga acgtcagcgg
      601 ggtgatgaac tgcgtcgcgt tttcggtcat gatgacatgc acatccgctc cggctttccg
      661 aagagcgctt gccacgtttg ccattttata ggcggcaatt ccgcccgtca caccaagtac
      721 aacacatttt ccgtcaagat tttccattgt aactccgttc tgctgcctcg aagcaacttt
      781 tttcctatga tagttttaag tatagcagac agaaaaacag attacaacca atcctgtttg
      841 ccattgatgc aaaaaggctt tataataaaa ggacaaaaga gctctacttt taaaataatc
      901 gcgaggtatt tttatgattt tagccattga tatcgggaac acaaccgtta cgctcggcgg
      961 cgtccggatc gcaccgcagc aagcgtattc cctcgaattc acagcccgga ttgacatcaa
     1021 ccacacctgg tctgccagcc agtatgaatc tgcgatcgag cagctttttg aagacaagaa
     1081 tctttccccg tccgactttc ggggcgtcat catttccagc gttgttccgt cggttctgac
     1141 gcttctgcag gaatgtgtcc gtaatctgtt cggcacagaa ccgctgctcg tcacatccga
     1201 aagtgatctc ggcatgacat tttccatccc ggaaccggaa aaagtaggcc gcgaccgcct
     1261 cgttgatgca gcctgggccg ccgcacatta tccgctgccg gtcatcaccg tggatcttgg
     1321 aacagcgacg accttcaatg tcatccggga aaacagcgtc ttctgcggcg gcgccatcgc
     1381 accgggactc gatatgggac ttggcgccct ctccgagcgg accgcccagc tcccgcggct
     1441 cgacctccag atcccgaaac gcatcattgg cagaaacacc gaagaatgca ttctctccgg
     1501 caccatcgtc ggcatggccg cgatgctcga cggaatggtc cagcgcatcg aagccgaact
     1561 cggctccccc gccaccctca tcctcaccgg cggcgcggca cgcttcgtcg aaccgctcgt
     1621 tttacatcct catatctatg acccgaacct tctgctgaaa gggctggcgt tcctgtgtga
     1681 gcggaattgt gcaaattaaa atcgtacttc caaatattca tcagactttt tcattctgca
     1741 tgacgttaaa aagcgtgtct gttctccagg cacgcttttc tctatttttc tatggaatcg
     1801 caacccttct ttgtgttgcc tctccgcttt tcagtttttc ctgattctcc tctttcagtt
     1861 ttaaaataca cgggcgaatg ctttcatcat acgtttctgt tgtctgaagt acatgaccat
     1921 tgtatacaat cacatcatcg gaatacagaa atacaagcgc catctgattc atctcatgat
     1981 cgtagatcag aacataccag ttataatcat acaaaatcct ctctgtcatg ttgaatacct
     2041 gttttggctt ctggcaggaa attttttctc cgtaaatggt cgagagaatt tcgtccatct
     2101 cctccggatg atcctcgcgc gtgatcgcaa aggacattcc ggctccggtg tcttccggga
     2161 tataaagata aatcatctcg gctttgtccg tatcaaaggt ttcgatttcc tgcagcagtt
     2221 cctgatccga tttttctgag atgaaatctg ctgccgtcag gaaaacatct gtcgtacccc
     2281 gctcaggacc aatgggattt tctttctgtg tatacaccgc cgtcattctt tttaccagac
     2341 atcccgcaat cacgcaaagg atcactccag ctaacatagt gatttttctc tttttcatac
     2401 gtttcaagga aatcacctct cttccaaaac agacattccg ggaaccttcc aaaagttgaa
     2461 ttttctcttt tcagtatatc ataaattttt ccgggacaca ctcttttcct tctccctatg
     2521 cacggaaaaa ctcccggcac tgccgggagt ttatgacttt cttatttcat attacttttt
     2581 cacctgctca tataccagtc ccggataagc ttcgaggctg acaccgtttt tttcaaacat
     2641 tggaaatgct tctgcgccgg atgcgcatac ggcggttgcc atgcttgctt gttcggtgta
     2701 gttctcccag ttgtcggtgg taaggccagg ctggtatgct tcccaggcag tttcgtattc
     2761 ggtgctctta tcggaaggaa tcgcatcttt caggctctcg ccggaccaga cggcaagctg
     2821 atctgctttt ttgtaagtgt attccgggag ctgatcgtcg cccagcgact ggtacagctc
     2881 gtagcgggta atggagtctt tggaacttcc gtaggatttt tccatttctt ttttcacggt
     2941 atccatatcg gcatcatccg ggtaggtgat gtctacttcg cacaataccg gttttcccgt
     3001 cgctccggtc tgatcgaggt tgataaactg aaacatgact ctggaagttt gctcgccgaa
     3061 aacagtggct tctgtatcta ctgcgatcgc ggctgcactt tcggtctcag agaaaatttc
     3121 cacctgagcc cgatctaaat tccagtcggc aagcacttca tccactgttt ttccccatcc
     3181 ggtatccggc ggcgtaattt ccttgctgct ctctttcgct ccctgacatc cagtcagcaa
     3241 cagagcacac attgcaaaaa tacctgctaa ttttttattc atacacctgc atctcctttc
     3301 cactttgtta tttttttcac atactttttt tgttagccct atactaccat atattcttct
     3361 gaaaatctat gacgtttggt gaaaattttc ttacattttt cttatgcatt cgatttaaat
     3421 caaagaaaaa gcaacaacat cccttataga aattctctac cgctagtgaa cctcacatga
     3481 ctaaagtcac gtgcttccgg ccgctttaag cggcagacca aatatcggcg gatacgcctg
     3541 tt
//
LOCUS       DAOMUF010000201         3535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7506_length_3535_cov_2.822701, whole genome shotgun sequence.
ACCESSION   DAOMUF010000201 DAOMUF010000000
VERSION     DAOMUF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7506_length_3535_cov_2.822701"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLN77_09425"
     CDS             <1..322
                     /locus_tag="PLN77_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM44523.1"
                     /translation="RREVEQIKQYIRENYSDSGMGVDQLAREVGMTPNYLSSIFKKNT
                     GENLSRYLKGFRMERAREMLENTNEKIGVICEKCGFVNVSYFCQSFREYFGISPQKYR
                     DQGE"
     gene            390..2054
                     /locus_tag="PLN77_09430"
     CDS             390..2054
                     /locus_tag="PLN77_09430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM44524.1"
                     /translation="MAYVLTGLFPMCLMFTLFYTQMRRVLVEREIKNLQTTFDQAVDD
                     VNTELALHQSMSDYLAFDQTIVQIVKAEDKNSFEAYERMVKEFDPMMDSLSYFYPEIR
                     QSTVYVRDFVIPHGTYLRPAREMENDEWTAPADNDVHWYADMDQGTVTLVRSMPLIDD
                     GKGGFLYISMDYSKIFGSMELTVNEDYGVFVYNEDKEVLYENQKMTRNAKYQMEFSDF
                     QKIQKKEKQNTANYILLEKEIEQNGWHAACYLPKKGIISPMQPILLMVLLMWAGCVIF
                     SAVVIMKFSGNMAHRLEKIKMTMSEVEEGNLEVELVSTERDEIGDLTRGFNQMLSRIR
                     QLIQEVYESRIRQKQYEMTALRAQINPHFLYNSLSLINWKALEIGSDDISKATLALSR
                     YYRTSLNKGKNTMSIREEIDNVRSYLQIQEMFHDYSFTVKMDVAEDILDYRTLNLILQ
                     PLAENAIAHGIDRRRGSEPGVITISGRRDGNCVVLSVADNGVGMEQEKAQTILTEKSS
                     GYGVRNVNSRIQLEYGEAYGLSIESEPGKGTKVTVRIPGVEREVQA"
     gene            2051..>3535
                     /locus_tag="PLN77_09435"
     CDS             2051..>3535
                     /locus_tag="PLN77_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM44525.1"
                     /translation="MMQKKLAAGLAVLLAAGMALSSCGESGAAGNDSVSDAAGNEAAA
                     LTEAKTTPYGRYPETITYTLAKMTGVNNSNLPEGETYEDNAYTRLIREIINVQNEDVY
                     ENYGDTYNVGISTMIATGNIADIMVVDQKTMNAMQKNDQLADLTEVYANCASDRIKDI
                     YASYGEEILQGCTFDGKLMAFPETNISDGPNLLWVRKDWMEKLGLSVPETIDDVKHIA
                     LTFAEENPANQEMGNIGLAVSTTLYGGTGISSEYGMNLIFASFGAYPGRWLTDAEGMP
                     VYGSVQPNVKDALGMLADWYQEGVLDRDFLIRTQDDIAELIAQGRCGIFFAPWWAPNN
                     PLWRCHETDPEADWQPFLIRTGKDGSVRYCNEKLTGNYVVVRKGYEYPEIVPKILSVM
                     FDYMRYSYDDPRGEFQQYYTGNIDPTARPLAINLDYNQALTICYENLQAALNGEKSED
                     ELEILERSFEKVCRAYLENPKTASAEEWSAYLSRITACSLLSDEK"
BASE COUNT          993 a          736 c          986 g          820 t
ORIGIN      
        1 acgcagagaa gtcgagcaga tcaaacagta tatccgtgag aactattctg acagtggcat
       61 gggtgttgac cagcttgcgc gcgaggttgg catgacaccg aattatctga gcagcatttt
      121 caagaaaaat acaggagaaa acctgagccg ctacttaaaa ggtttccgta tggagcgcgc
      181 acgggaaatg ctcgaaaata caaacgaaaa aatcggcgta atctgtgaaa agtgcggttt
      241 tgtgaatgtg tcctatttct gtcagagctt ccgcgaatat tttggaatca gcccgcagaa
      301 ataccgggat cagggggagt aagatgacat catttgccgc tagaataaaa aaagcatttt
      361 cagatatgaa ggtacaccgg aagcttatcg tggcttacgt tctcacagga cttttcccaa
      421 tgtgcctgat gttcacactg ttttacaccc agatgcgtcg tgtacttgtc gagcgtgaga
      481 tcaaaaatct gcaaaccacc tttgatcagg cagtggacga tgtgaatacg gagcttgcgc
      541 tgcaccagag tatgtcggac tatctggcat tcgaccagac aattgttcag attgtaaagg
      601 cagaagacaa aaacagcttc gaagcatacg agcgcatggt gaaagagttt gatccgatga
      661 tggattcctt aagttatttt tacccggaaa tccgacagtc tacggtctat gtgcgtgatt
      721 tcgtcattcc gcacggcacg tatctgcgtc cggcgcggga aatggaaaat gatgaatgga
      781 cagcaccggc agacaatgat gttcactggt atgctgatat ggatcaggga acggtgacgc
      841 tggtgcggtc tatgccgctg attgatgacg gaaaaggcgg ttttttatac atttccatgg
      901 actacagtaa gatttttggc tccatggagc ttacagttaa tgaggattac ggtgttttcg
      961 tctacaatga ggacaaagaa gtgctgtatg aaaatcagaa gatgaccagg aatgcaaaat
     1021 accagatgga attttcagac tttcagaaga tacaaaaaaa agagaagcag aatacagcga
     1081 actacattct cctggaaaag gagattgagc agaatggatg gcatgccgcg tgctatctgc
     1141 cgaaaaaagg catcatcagt ccgatgcagc cgattcttct gatggttctt ctgatgtggg
     1201 caggctgcgt tattttctct gcggtcgtga ttatgaaatt ctccggaaac atggcgcacc
     1261 gccttgaaaa gataaaaatg acaatgtccg aggtagaaga aggaaatctg gaagtggaac
     1321 tggtttctac agaaagggat gaaatcggtg atctgacgcg tggctttaat cagatgctgt
     1381 cacgcatccg tcagctgatt caggaagtct atgagagccg catccgccag aaacagtatg
     1441 agatgacggc gctccgcgca cagattaacc cgcattttct gtataattcc ctgtcgctga
     1501 tcaactggaa agcgctggaa atcggttcgg atgatatcag caaggcgacg ctggcgcttt
     1561 cgcggtacta caggacttcc ctgaacaaag gcaaaaatac gatgtccatc cgcgaggaga
     1621 tcgacaatgt gcgttcgtat ctgcaaattc aggaaatgtt tcatgactac agctttacgg
     1681 ttaaaatgga tgtagcggag gatattctgg attaccgtac attgaacctg attctgcagc
     1741 cgcttgccga aaatgcgatt gcgcatggaa ttgaccggcg gcgcggcagt gagcccggcg
     1801 tgatcacaat cagcggacgg cgtgacggaa attgtgtggt actttccgtg gcagataatg
     1861 gtgttggtat ggaacaggag aaagcacaga caattttgac ggagaagagc agcggctacg
     1921 gtgtgcggaa tgtcaattcc aggattcagc tcgaatacgg agaagcgtat ggtctttcga
     1981 ttgagagtga gccgggaaag ggaacaaaag tgacggtgcg gattccgggc gtggaaaggg
     2041 aggtacaggc atgatgcaaa aaaaacttgc ggcaggcctt gctgtactgc tggcagcggg
     2101 aatggcgctg agcagctgtg gagaaagtgg cgcagcagga aatgattctg tttccgatgc
     2161 tgccggaaat gaggctgcgg ctcttacgga agcgaaaaca acgccgtacg gacgttaccc
     2221 ggaaaccatc acgtacacgc tggcaaaaat gacgggtgtg aataattcca atctcccgga
     2281 gggagaaacc tatgaggaca atgcctatac acgcctgatc cgcgaaatca ttaatgtaca
     2341 gaacgaggat gtttatgaaa actacggtga tacctataac gttggcattt ctaccatgat
     2401 tgcaacagga aacattgccg atatcatggt tgtggatcag aaaacgatga atgccatgca
     2461 gaaaaacgat cagcttgccg atcttacaga ggtttatgca aattgtgcca gcgaccgtat
     2521 caaggatatt tatgcaagct acggtgagga gattctgcag ggctgtacct ttgacggaaa
     2581 gctgatggca ttcccggaaa ccaacatttc ggacggtccg aatcttctat gggtgcggaa
     2641 ggactggatg gaaaaactgg gactttccgt accggaaacg atcgatgatg tgaaacatat
     2701 tgcgttgacg tttgcagagg agaatccggc aaaccaggag atgggtaaca tcggtcttgc
     2761 agtcagcaca actctgtacg gcggcacggg catttcttcg gaatacggca tgaatcttat
     2821 ttttgcctca tttggtgcat atccgggaag atggctgacg gatgcggaag gaatgccggt
     2881 ttatggttcc gttcagccca atgtaaaaga cgcacttggc atgctggccg actggtacca
     2941 ggagggcgtc cttgaccggg actttttgat ccgcacacag gatgacattg ccgagctcat
     3001 tgcccaggga cgctgcggca ttttcttcgc accgtggtgg gcaccgaaca acccgctctg
     3061 gcgctgtcat gagacggatc cggaggccga ctggcagccg tttctgattc ggaccgggaa
     3121 ggatggaagt gtgcgatact gcaacgaaaa gctgaccgga aattatgtgg tagtgcgaaa
     3181 agggtatgaa tatccggaga ttgtgccgaa gattctgtcg gtgatgtttg attatatgcg
     3241 ctattcctac gatgatccaa ggggagaatt tcagcaatat tatacgggaa atatcgatcc
     3301 gacggcacgc cccttagcaa tcaatctcga ctataatcag gcactgacga tctgttacga
     3361 aaatctgcag gcggcactga acggggaaaa atcggaggat gagctggaga ttttggaaag
     3421 atcgttcgaa aaggtatgcc gcgcctatct ggagaacccg aaaacagcga gtgcggagga
     3481 gtggagcgcg tatttatccc gtatcacagc ctgctcgctt ttaagcgacg aaaag
//
LOCUS       DAOMUF010000202         3526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7528_length_3526_cov_3.213483, whole genome shotgun sequence.
ACCESSION   DAOMUF010000202 DAOMUF010000000
VERSION     DAOMUF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7528_length_3526_cov_3.213483"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLN77_09440"
     CDS             complement(<1..202)
                     /locus_tag="PLN77_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44526.1"
                     /translation="MDGKNREEQENGAKVRDLEEYKAENRKRKRRRRITVGILAGAIL
                     AAGIGTGVWLQLRTFQGYDTVQA"
     gene            complement(291..1598)
                     /locus_tag="PLN77_09445"
     CDS             complement(291..1598)
                     /locus_tag="PLN77_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRM44527.1"
                     /translation="MIEIVYKENKEQANGNEGYFHLPKNIRQVGDTNGRERIYLEDYV
                     GTFLKKYFSAPEGRAAMLFGEFKWADGISYFFIRSAAAIHTMEPAPDHLVFDDAVWTE
                     VHDVMEKYFKNQQILGWALSLPGYGEDLNEQIIRAHLNHFGGNDKTLFRVNGQEKEET
                     FYTYEGGTLRSTGGFYIYYEKNEPMQSYLIDQNQNRSIENGESVPDRAVSDFRKMIEE
                     KSGEEPEHDHRKPILYAMTACAAAALLVTVTQKYGDALPFPGKTKEQTVAVAGNVQGE
                     KTQTAESAAEASAAEEGNTGENTSTPESTGTELETENESGSAAVSEEQNLSGTDPAGS
                     GGQSTSGGEQTSAAENNTGTAQTDPAPGAEPSSAPGTASGSETAETAAQVWQEYTVQK
                     GDSLSKISERYYGTMSRVKDICELNQIAVEDLIYAGQKIYLPK"
     gene            1799..>2103
                     /locus_tag="PLN77_09450"
     CDS             1799..>2103
                     /locus_tag="PLN77_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1256 domain-containing protein"
                     /protein_id="HRM44528.1"
                     /translation="MFRKKDAEVYYINERSRSASEELASLFSYCIQKDGRMFRELVFL
                     CIGSDRITGDSLGPLIGYQLSPYCSRVFHVYGTLDDPVHALNLPDRISYIHSRHPEAL
                     "
     assembly_gap    2104..2113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2114..2393
                     /gene="yyaC"
                     /locus_tag="PLN77_09455"
     CDS             <2114..2393
                     /gene="yyaC"
                     /locus_tag="PLN77_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HRM44529.1"
                     /translation="VAIDASLGSRRHQGYITIGNGAIRPGAGAGKTLPEVGDIFITGI
                     VNVSGSFEQLLLQTTRLATIFHMAESISQGILLACRELEAAGTSELLL"
     gene            complement(2485..3426)
                     /locus_tag="PLN77_09460"
     CDS             complement(2485..3426)
                     /locus_tag="PLN77_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRM44530.1"
                     /translation="MSEVKKIATRESYGNALVELGAEHDNLIVLDADLAAATKTGVFK
                     KAYPDRHIDCGIAECNMMGIAAGLSTTGIVPFASTFAMFAAGRAFEQVRNSIGYPHLN
                     VKIGATHAGISVGEDGATHQCNEDIALMRTIPGMVILNPADDVEAKACVKAAYEYNGP
                     VYLRFGRLAVPVINDRPDYKFELGKGVVLREGKDVTIVATGLCVSSALEAAEKLAADG
                     IDAKIINIHTIKPLDEELIVAAAKETGKVVTVEEHSVIGGLGSAVCDALAEKCPVPVK
                     KIGVQDVFGESGPAAALLAKYKLDGEGVYEQVKEFCK"
     gene            complement(3414..>3526)
                     /locus_tag="PLN77_09465"
     CDS             complement(3414..>3526)
                     /locus_tag="PLN77_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRM44531.1"
                     /translation="SFMENQVGWHGKAPNDEEYAIAMAELEKAGEALCQK"
BASE COUNT          648 a         1009 c          838 g         1021 t
ORIGIN      
        1 ttgcctgtac tgtgtcgtat ccctgaaaag tacgcagctg aagccatacc cctgtaccaa
       61 ttccggccgc gagaatggct cctgcaagga ttccaacggt gatccggcgt ctgcgttttc
      121 tttttctgtt ttctgcttta tactcctcaa gatcccggac ttttgctccg ttctcctgtt
      181 cttctctgtt ttttccgtcc attttcattc cccttatgtt cttttcggac ttattttctg
      241 ccgccctttc ggccgcagtt tgattccgct ctagtgtacc atatttttct ctattttggg
      301 aggtatattt tctgccccgc atatatgaga tcttccaccg cgatctggtt cagttcgcag
      361 atatctttca ctctgctcat cgttccgtaa tatctctcac tgattttcga gaggctgtcc
      421 cccttctgta cggtgtactc ctgccagacc tgcgcggcgg tttctgccgt ctcactgccg
      481 gaagcagttc ccggggcgga cgatggctct gctcccggtg ctggatctgt ctgcgccgta
      541 ccggtattgt tctccgctgc actagtctgc tccccgccgg atgtgctttg ccctccgctg
      601 cctgccggat ccgttccgga taaattctgc tcctcactta ccgccgcgct cccgctctca
      661 ttttccgttt ccagttccgt tcctgtgctt tccggtgtcg aagtattttc ccctgtgttt
      721 ccctcttccg ccgctgatgc ttccgctgca ctctccgccg tctgtgtctt ctctccctgt
      781 acgtttcccg ccactgctac cgtctgctcc tttgttttcc ccggaaacgg cagcgcatct
      841 ccgtatttct gcgtcaccgt cacaagaagc gccgcagccg cgcatgctgt catcgcatag
      901 aggatcggct ttctgtgatc gtgttccggc tcctccccgc tcttctcctc aatcatcttc
      961 cgaaaatcgc tgacagcgcg gtcgggcacg ctctctccgt tttcgatcga ccggttctga
     1021 ttctgatcga tcagatacga ctgcatcggc tcattctttt cataataaat gtaaaagcct
     1081 cccgtgctcc gcagggtgcc gccctcgtac gtatagaagg tttcctcttt ttcctgtccg
     1141 ttgacgcgga acagcgtctt gtcatttccg ccgaagtggt tcagatgcgc tcggatgatc
     1201 tgttcattca gatcctcccc gtatcccgga agagaaagtg cccagccgag gatctgctgg
     1261 tttttgaaat atttttccat cacatcgtgg acctccgtcc atactgcatc atcgaagacc
     1321 aggtgatccg gcgccggctc catcgtatga atcgcggccg cgctgcggat aaaaaagtag
     1381 gaaattccgt ccgcccactt aaattcgccg aacagcatgg cggcacgtcc ctccggtgcg
     1441 gaaaaatatt ttttcagaaa ggttccgaca taatcttcca gataaatccg ctccctgccg
     1501 ttcgtatcac cgacctggcg gatatttttc ggcagatgaa agtatccctc gttgccgttc
     1561 gcctgctctt tgttttcttt atagacaatc tcaatcatgc ttttcacccc tttttgccta
     1621 ctttatcacg gatgttccgc gaaaaagatc gttttggggc agggctgtca cggttttttc
     1681 cgactgtttt cccgtttttc tgcaccggag ggcatgtcat gtttttttca ttgcaggaat
     1741 agtttatacc atatacggtt aaactgtgga tgattcttct gaaaggcagg taccggtcat
     1801 gtttcgaaaa aaagacgccg aagtttacta catcaatgag cgaagccgca gcgccagcga
     1861 agagctggcg tcgctgtttt cttactgcat tcaaaaggac ggacgcatgt tccgtgaact
     1921 ggtatttctc tgcatcggaa gcgaccggat cactggggat tccttaggac cgctgatcgg
     1981 gtaccagctt tcgccgtact gcagccgcgt ttttcatgtc tatggaacgc tggatgaccc
     2041 ggtgcacgcc ctgaatctac cggaccggat ttcgtacatc cattcccgcc acccggaggc
     2101 actnnnnnnn nnncgttgcg atcgatgctt cgcttggttc gcggcgtcat cagggatata
     2161 tcacgatcgg aaacggggcg atccggccgg gcgcgggggc agggaagacg ctgcccgagg
     2221 tcggggatat ttttatcaca ggcattgtga atgtatcggg cagttttgaa cagcttcttc
     2281 tgcagacaac ccggcttgcc acgattttcc atatggcgga atccatctcg cagggaatcc
     2341 tgctcgcctg ccgggaactt gaggcggccg ggacaagcga acttctgctt taagaaaaac
     2401 tgcagacaac aaaacaggca gagactgcac cgttttccgg tgtggcctct gcctgttttc
     2461 ttttgtcatt tatttcgaaa aatcttattt acagaactct tttacctgct cgtaaacgcc
     2521 ctcgccgtcg agtttgtatt ttgcaagaag agcggctgcc gggccggact ctccgaagac
     2581 gtcctgtaca ccgattttct tcaccggaac cgggcatttc tctgccagtg cgtcgcagac
     2641 tgcgcttcca agtcctccga tcacggagtg ctcttctacg gttacgactt tgccggtctc
     2701 ttttgctgct gcaacgatca gctcctcatc cagcggtttg atggtgtgga tgttgatgat
     2761 cttggcatca atgccgtctg cggcaagttt ctctgctgct tccagagcgg agcttacgca
     2821 aaggcctgtt gcaacgatgg tgacatcctt gccttcacgg agaacaacgc cttttccgag
     2881 ctcaaatttg taatccggtc tgtcgttgat caccggcact gccagacgtc cgaaacgaag
     2941 gtaaaccggt ccgttgtatt cataagctgc tttcacgcag gcttttgcct ctacatcatc
     3001 tgccgggttc aggatgacca tgcccgggat ggttctcatc agggcgatat cctcgttgca
     3061 ctgatgtgtt gcgccgtctt ctcctacgga aattccggca tgggtagcac cgatttttac
     3121 gttcagatgc gggtatccga tggagttgcg cacctgctca aacgcacggc ctgcggcaaa
     3181 catcgcgaag gtgcttgcga acggaacgat gcctgtggtg gaaagaccgg cagcgattcc
     3241 catcatatta cattctgcga taccacagtc gatgtgacgg tccggatatg cttttttgaa
     3301 cacaccggtc ttggttgctg ctgcaaggtc agcgtcgagt acgatcagat tgtcatgctc
     3361 tgcgccaagt tcaaccagtg cattaccgta actttctctt gttgcgattt tctttacttc
     3421 tgacataacg cttcacctgc cttttccagt tctgccatag caattgcgta ttcttcatcg
     3481 ttcggagctt ttccgtgcca gcctacctgg ttctccatga aggaaa
//
LOCUS       DAOMUF010000203         3500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_7602_length_3500_cov_3.135849, whole genome shotgun sequence.
ACCESSION   DAOMUF010000203 DAOMUF010000000
VERSION     DAOMUF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7602_length_3500_cov_3.135849"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="PLN77_09470"
     CDS             complement(<1..1069)
                     /locus_tag="PLN77_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRM44532.1"
                     /translation="MDYAKESLKMHYELRGKLEVTSRAAVDTKDALSLAYTPGVAAPC
                     LEIQKDVNKSYELTRRWNTVAVVTDGTAVLGLGDIGPEAGMPVMEGKCVLFKEFGGVD
                     AIPLCVRSKDVDDIVNTVRLLAGSFGGVNLEDISAPRCFEIEKKLKECCDIPIFHDDQ
                     HGTAVITLAGVINALKLVGKKLDEVKIVTSGAGAAGIAIIRLLISMGLKNVIMTDRKG
                     AIYEGRDGLNPIKEEMAKITNYNHEKGTLAEVIKGADIFIGVSAPGTLTPDMVRTMAK
                     EPIIFACANPTPEIFPDEAKAAGAAVVSTGRSDYPNQVNNVLCFPGIFRGALDVRASD
                     INDEMKVAAAYAIAGLVSDEEL"
     gene            complement(1418..2806)
                     /locus_tag="PLN77_09475"
     CDS             complement(1418..2806)
                     /locus_tag="PLN77_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44533.1"
                     /translation="MQKHRKFIGDKRFYLMVLGVAMPIMIQNGITNFVSLLDNIMVGQ
                     VGTEQMSGVAIVNQLLFIYNLCIFGGVSGAGIFTAQYFGQGNDEGVRNTFRFKLWIGV
                     ILTAAAITIFLGAHEPLIRLYLTGDQGSQDMNATLAYGISYMKVMLVGLPPFLFVQLY
                     ASTLRECGETMLPMKAGITAVFVNLIFNYILIYGKFGAPQLGVVGAAVATVLSRYVEM
                     AIVLIRTHGNKEKYRFTKHLYRTIRVPAALTKQILIKGTPLMLNETFWAGGVAILMQC
                     YSVRGLNVVAAMNISNTISNLFNVVFIALGDSVAIIVGQLLGAGRMEEAKDTDTKLIT
                     FSVLLCIVIGALMALVAPIFPRLYNTTDEVRHMAAIFIVEVAMFMPVNAFLHASYFTL
                     RSGGKTIITFLFDSVFIWVVSVPVAFCLSRFTGLSIYAIYFMVMSVDLIKCVIGFVLV
                     KKGVWLQNIVAE"
     gene            complement(3154..>3500)
                     /locus_tag="PLN77_09480"
     CDS             complement(3154..>3500)
                     /locus_tag="PLN77_09480"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM44534.1"
                     /translation="TGRGDEGGSGAGGKICGMLWDDFLNSKVNAFQDVLNSKIYINKT
                     GLLEYTNSVIDTTSKFICNSRPRRFGKSITADMMTAYYSRSLDTEEMFEKLNIGQAAN
                     QKIQDEYQTADS"
BASE COUNT          881 a          918 c          755 g          946 t
ORIGIN      
        1 tcagctcttc atcgcttacc aggcctgcga ttgcgtaagc tgctgcgacc ttcatttcat
       61 cgttgatatc agatgctctc acatcgagcg ctccgcggaa gatgcccggg aagcagagta
      121 cgttgttgac ctggttcggg taatcgcttc ttcctgtgct gacaacggct gctcctgctg
      181 ctttcgcctc gtccgggaaa atctccggcg tcggatttgc gcaggcaaag atgatcggct
      241 cttttgccat cgtacgtacc atatcgggag tgagcgtacc cggcgcagaa acgccaataa
      301 aaatatcggc tcctttgatt acctctgcaa gcgtgccctt ctcatggttg tagtttgtga
      361 tcttcgccat ctcctctttg atcgggttga ggccgtcgcg tccctcgtaa atcgcacctt
      421 tgcggtctgt catgatgacg tttttgagac ccatcgaaat cagcaggcgg ataatggcga
      481 ttccggctgc accggctcct gaggtaacaa ttttgacttc atccagtttc tttccaacca
      541 gtttcagcgc attgatgaca ccggccagtg tgatcacggc tgttccatgc tgatcatcgt
      601 ggaaaatcgg aatatcacag cattccttta atttcttctc gatctcaaaa catctcggtg
      661 cggagatgtc ctccaggttg acaccgccga agcttcctgc gagaagacgc acggtattga
      721 caatatcatc cacatccttg ctgcgcacgc agagcgggat tgcatcgaca ccaccgaatt
      781 ctttaaacag cacgcatttt ccttccatga ccggcatgcc ggcctccggg ccgatatctc
      841 caagtccgag aactgcggtt ccatctgtaa caacagctac cgtgttccat cttctggtca
      901 gctcgtagct cttgttgaca tctttctgaa tttccaggca cggcgccgcc acacccggtg
      961 tataggcaag actcaaggca tcttttgtat ctacggccgc ccgggaagtg acctcaagct
     1021 ttccgcgtaa ttcatagtgc atctttaatg attcttttgc gtaatccatc gctttttttc
     1081 ctccatgtgg tgtatgaacg cgcctcatta ataataagaa gagagattca cgcgtcatac
     1141 gagatgtctg ttcgctgcac ccctattata acaagcgttc tctaaatttc aaccggtgtt
     1201 ttgattaatg aaaaaatttc cacttttcca ccaaaaacag ctgcttcatt tgcgaaattt
     1261 tatcaaaaat actctttttc tttctctcag ccgcagtccg atcctgcccg gagggctttt
     1321 tttgctttac agtaaaattc aacaaagttt tttataaagc aaaaaatctg cccgcctccc
     1381 atccttttca gactggaaaa cagacagatt tttttcttta ctccgccaca atattctgca
     1441 gccagactcc tttcttgaca aggacaaatc caatcacgca cttgatcagg tcaaccgaca
     1501 taaccataaa gtagattgcg tagatcgaaa gtccggtaaa acggctcagg cagaaagcca
     1561 ccggcacact gacaacccag atgaagacgc tgtcgaacag gaacgtgatg atcgtttttc
     1621 caccggagcg cagggtaaag tagcttgcat gcaggaatgc attgaccggc atgaacatgg
     1681 ccacttccac gatgaagatt gccgccatgt ggcgtacttc gtccgtcgtg ttgtagaggc
     1741 gtgggaagat tggagccacg agcgccatca gcgcgccgat cacaatgcag agtaatacgg
     1801 aaaacgtgat cagcttcgta tcggtatcct ttgcctcttc catccttccg gctccgagga
     1861 gctgtccgac gatgatggca acggaatcgc cgagtgcgat gaaaacgacg ttgaacaggt
     1921 tcgagatcgt gttggagata ttcatcgcgg ctacgacatt cagtccacgc acggagtaac
     1981 actgcatcag gatcgcaacg ccgcctgccc agaacgtttc gttcagcatc agcggtgttc
     2041 ctttgataag gatctgtttc gtcaaagccg ccggaacacg gatcgtgcgg tacagatgct
     2101 tcgtaaaacg atatttttct ttgtttccgt gcgtgcggat cagcacgatc gccatctcca
     2161 cgtaacggga cagcaccgtt gcgactgccg cgccgacaac gccgagctgc ggtgcaccga
     2221 attttccgta gattaaaatg tagttaaaaa tcagattaac gaagacagcg gtaataccgg
     2281 ccttcatcgg aagcattgtc tcgccgcatt cgcgcagcgt gctggcataa agctgcacaa
     2341 acaggaacgg cggaagtcct accagcatga ccttcatata agaaatgcca taggctaatg
     2401 tcgcattcat gtcctggctt ccctgatctc ctgtcaggta gagacgaatc agaggctcgt
     2461 gcgcaccaag gaaaatagtg attgccgctg ccgtcaggat cacgccgatc cagagcttga
     2521 agcggaaggt gtttcgcaca ccctcgtcgt tgccctgtcc gaaatactgc gcagtgaaga
     2581 ttcctgcgcc agacacgcct ccaaagatac agaggttata gataaaaaga agctgattga
     2641 cgatcgcaac accggacatc tgctcggtgc cgacctgtcc taccatgatg ttgtcgagaa
     2701 ggctgacaaa gttggtgata ccgttctgaa tcataatcgg catcgcaacg ccaagcacca
     2761 tcaggtaaaa ccgtttgtcc ccaataaatt ttctgtgttt ctgcatggtt tccccctttt
     2821 cttgttgtat aaaataaatg acataaatcg gagcctttct ggatttctcc aggcaggccc
     2881 cgaacagtaa tgcttattat acacgattca tgtttagaaa gaaagatcct ttttatcttt
     2941 tttctggctt tttttgacat cttaatttca gagcatcaaa cagcctcaac gataagataa
     3001 gcataactcc ccatattacc catgccgcaa agcacccaat tccctgatgg cggatatcta
     3061 ccattattcc aacaataaaa ggaagtgaca taaacataat tactgccatt ataaaatcaa
     3121 aaataatgta taacactatg tttaccttcc ctattaagaa tctgctgttt gatattcatc
     3181 ctgtattttc tgatttgccg cctgaccaat attaagcttt tcaaacattt cttctgtatc
     3241 cagacttctg ctgtagtacg cagtcatcat atccgctgtg attgattttc caaatcttct
     3301 ggggcggctg ttgcagataa acttcgatgt tgtgtcaatc acactgttgg tgtattccag
     3361 caatcctgtt ttattgatat atatttttga gtttaatacg tcctgaaaag cattcacctt
     3421 tgaatttaaa aaatcgtccc acagcatgcc gcaaattttt ccaccagcgc cactgccacc
     3481 ttcatcaccc cggccagtac
//
LOCUS       DAOMUF010000204         3366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8019_length_3366_cov_4.920870, whole genome shotgun sequence.
ACCESSION   DAOMUF010000204 DAOMUF010000000
VERSION     DAOMUF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8019_length_3366_cov_4.920870"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2992)
                     /gene="mfd"
                     /locus_tag="PLN77_09485"
     CDS             complement(<1..2992)
                     /gene="mfd"
                     /locus_tag="PLN77_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRM44535.1"
                     /translation="MKALLEPIKEIARFDELFRDLKKGTAGVTVISGCLESQKAHMIA
                     GLSEDAPVRLLIAENELKAKELYEDYRLYDAETLYYPAKDLIFYQADIHGNLLIRQRL
                     QVIQALLERKEVTIITSVGGCMDFLLPLGVFEKHCIHLRNDSELDLDKIRKELVRLGY
                     ESTFQVESTGQFSVRGGILDIYPLTEQNPIRIELWGDEIDSIRSFDAESQRSIENLEE
                     ITIYPASELILTENTIARGVKAIKKEAKAQSTKFRDKMMTEEAARIRHAAEEICDQIG
                     EGEILQGAEQYLHYFYPETVSFLEYLPENSRIFLDEPNRIIECADALEAEFQESVSHR
                     LEKGYLLPGQAKLMCSTAELVAKLNRRGCVAVSLMEPQKGEWNIRDEFSVTVKSVNSY
                     NNQFPLLVKDLKSWKKAGYRIVLASPSRTRAQHLAEDLQNEEIPAFYSENEERVLKPG
                     EVMLIHGAARRGFEYPMQKFVLITETDIFGKEKKKNRKKSEYSGQKIQNFADLSIGDY
                     VVHESHGLGIYRGIEKIEMDRVTRDYMKIEYAGGSNLYVLATQLDTLQKYASGEAAKV
                     PKLNKIGGQEWNKTKTRVRHAVKNIAQDLVKLYAERQASSGYQFGKDTIWQKEFEELF
                     PFEETDDQLAAIEATKADMESKKIMDRLICGDVGYGKTEVAIRAAFKAVQESKQVVYL
                     VPTTILAQQHYNTFVERMKDFPVRVDLLCRFRTPAEQKKTLEDLKKGLVDIVIGTHRV
                     LSKDVKFKDLGLLIVDEEQRFGVTHKEKIKQLQKNVDVLTLTATPIPRTMHMSLIGIR
                     DMSVLEEAPQDRQPIQTFVCEYNEEMVREAINRELARGGQVYYVYNRVQSIADMANTI
                     QKLVPDANVGFAHGQMKERELEKIMYGFINGEIDVLVSTTIIETGLDISNVNTMIIHD
                     SDQMGLSQLYQLRGRVGRSNRTSYAFLMYRRNKMLKEVAEKRLHAIREFTEMGSGFKI
                     AMRDLEIRGAGNLLGAEQSGHMESV"
     gene            complement(2998..>3366)
                     /gene="pth"
                     /locus_tag="PLN77_09490"
     CDS             complement(2998..>3366)
                     /gene="pth"
                     /locus_tag="PLN77_09490"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRM44536.1"
                     /translation="RELVNYYKVDPESELIVVYDDISLAPGQIRIRKKGSAGGHNGIK
                     NIIANLGTDRFMRVKVGVGEKPKNWDLADYVLSPFTKDERPLVNLAIEHAAKAIEQML
                     NGDVDAAMNEYNRKSANPAE"
BASE COUNT          702 a          994 c          755 g          915 t
ORIGIN      
        1 caaccgattc catatggccc gactgctctg cgcccagcag atttccggcg cctcggatct
       61 caagatcacg cattgcaatt ttaaagccgc ttcccatctc cgtaaattca cggatcgcat
      121 ggagacgctt ctccgccact tccttcaaca tcttgtttct gcggtacatc agaaatgcgt
      181 aggaagtccg gttcgaccgt ccgacgcggc cgcggagctg atagagctgt gacagtccca
      241 tctggtcgga atcatggata atcatcgtgt tgacattcga aatatcgagt cctgtctcaa
      301 tgatcgttgt tgacacgagc acatcgatct ctccgttgat aaagccgtac atgatctttt
      361 ccagctcacg ctctttcatc tgtccgtgcg caaagcctac atttgcatcc ggcacgagct
      421 tctggatcgt gtttgccata tccgcaatcg actggacacg gttatagaca tagtaaacct
      481 gcccgccccg cgcaagttca cggttaatcg cctcgcggac catttcttca ttgtattcac
      541 agacaaaggt ctggatcggc tggcgatcct gcggtgcttc ctcgagcaca ctcatatccc
      601 ggatgccgat caggctcatg tgcatggtac gtggaatcgg cgtcgccgtc agtgtcagaa
      661 catccacatt tttttgcagc tgtttgattt tttctttatg cgtcacgccg aaccgctgct
      721 cctcatcgac aatcagaagg ccaagatctt tgaacttcac atcctttgaa agaacgcggt
      781 gtgttccaat gacaatatcc acgagtcctt ttttcagatc ctccagcgtt tttttctgct
      841 ctgccggcgt gcggaaccgg cacagaagat caacgcgcac cggaaagtcc ttcatgcgct
      901 caacgaacgt attgtaatgc tgctgtgcca gaatggtggt cggaacgagg tagacgacct
      961 gtttgctctc ctgtactgcc ttgaacgctg cacggattgc aacctcggtt ttgccgtatc
     1021 cgacatcgcc gcagatcagg cggtccatga tctttttgct ctccatgtcc gcctttgtcg
     1081 cctcgatcgc cgcaagctga tcgtccgtct cctcaaacgg aaacagttcc tcaaattctt
     1141 tctgccagat cgtatccttt ccgaactgat agccggatga cgcctgccgc tcggcataca
     1201 gcttcacgag atcctgtgcg atatttttta ccgcatggcg aacccgtgtt ttcgtcttat
     1261 tccattcctg accgccgatt ttattcagct tcggcacctt tgcagcttcg ccggacgcat
     1321 acttctgcag cgtatcgagc tgggtggcca gcacatagag gttcgatccg cccgcatact
     1381 cgattttcat gtaatcacgc gttacgcggt ccatctcaat tttttcgata ccgcggtaaa
     1441 taccaagacc gtggctctca tgcacgacat agtcaccgat cgatagatcc gcaaaattct
     1501 ggattttctg accggaatac tccgatttct tccggttttt cttcttctct tttccaaaaa
     1561 tatccgtctc tgtgatcagc acaaatttct gcatcggata ttcaaagcct ctgcgggccg
     1621 cgccgtggat cagcatgacc tcgcccggtt tcagaacacg ctcctcattc tcgctgtaga
     1681 aggccggaat ctcctcattc tgcaggtcct ccgccagatg ctgcgccctc gtccgcgacg
     1741 gactcgccag tacaatacgg taacccgctt ttttccagga tttcagatct tttaccagaa
     1801 gcgggaactg attgttatac gaattgaccg atttcaccgt cacactgaat tcgtcacgga
     1861 tattccactc gcccttctgc ggctccatca gcgatacggc aacacagccg cggcggttca
     1921 gttttgccac aagctccgcc gtactgcaca tcagcttcgc ctgtcccggc aggaggtatc
     1981 ccttttcgag acggtggctc acgctctcct gaaattctgc ttccagcgca tcggcacact
     2041 cgatgatgcg gttcggctca tcgaggaaaa tgcggctgtt ctccggcaga tactcaagaa
     2101 acgagacggt ctccggataa aaataatgaa gatactgctc cgctccctgc agaatttcgc
     2161 cctcgccgat ctgatcgcag atttcctctg ccgcatggcg gatacgcgcc gcttcctccg
     2221 tcatcatctt gtcgcggaac tttgtgcttt gcgcttttgc ctctttttta atcgctttca
     2281 caccgcgcgc aatcgtattc tctgttaaaa tcagctccga tgccggatag attgtgattt
     2341 cctcgaggtt ttcaatcgac cgctggctct ccgcatcgaa gcttcggatg gaatctatct
     2401 catcgcccca gagctcaatc cggatcgggt tctgttccgt cagcggataa atatcgagaa
     2461 tgccgccgcg gacggaaaac tggccggtcg actccacctg aaaggtactc tcatagccga
     2521 gccgcaccag ctcttttctt attttatcca gatcgagttc gctgtcattg cgcagatgga
     2581 tgcaatgttt ttcgaacacg ccaagaggca gaagaaagtc catacatccc cctacactgg
     2641 tgatgatggt aacttccttc cgctcaagaa gtgcctggat cacctgaagc cgctgccgga
     2701 tcagcagatt tccgtgaatg tccgcctgat agaaaatcag gtccttcgcc ggataataca
     2761 gcgtctccgc atcgtacagc cggtaatcct catataattc ttttgctttt aattcatttt
     2821 ccgcgatcag aagccgcacc ggggcatcct cggagagacc tgcgatcatg tgggctttct
     2881 ggctttccaa acagcccgag atcaccgtca cacctgcggt tccttttttc agatcacgga
     2941 acagctcgtc aaaacgggca atttctttga tcggttctaa taacgctttc attttcctta
     3001 ctctgccggg tttgcagatt tgcggttata ctcattcata gctgcgtcca catccccgtt
     3061 cagcatctgc tcgattgctt ttgccgcatg ctcgatcgcc agatttacaa ggggacgttc
     3121 gtctttcgta aacggactta acacgtagtc cgccagatcc cagtttttcg gtttctcacc
     3181 gacgccgacc ttcacacgca taaatcggtc ggtgccaaga ttggcaatga tgtttttgat
     3241 gccattgtgg ccgcccgcgc ttcctttttt gcggatacgg atctgtcccg gcgccaggct
     3301 gatatcatcg taaaccacga tcagctcgct ctccggatcc actttgtaat agttcaccag
     3361 ctcccg
//
LOCUS       DAOMUF010000205         3326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8148_length_3326_cov_3.518190, whole genome shotgun sequence.
ACCESSION   DAOMUF010000205 DAOMUF010000000
VERSION     DAOMUF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8148_length_3326_cov_3.518190"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="PLN77_09495"
     CDS             <1..1345
                     /locus_tag="PLN77_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44537.1"
                     /translation="NGLINIVLECFNLLFIVIFMFLVDVQMALVVLCGVPVLCVFMVW
                     IKNKQRRAWQAVSNKNSNLNAYLQENIVGARITQIFAREDENAEIFKDLSQDCRRTWN
                     TAVRYSNLVWPGIDSISVCVRAAIFLAGLVLFGQGSKSLGTIVAISSYASYFWQPIMN
                     LGNIFNNFINNIAYLERIFETMDEPVTVKDAENAVKMPEIRGEVTFDHVNFSYDESKQ
                     ILKDVSFTVKPGESVALVGPTGAGKSTIVNLISRFYNVNGGRVLIDGQDISQVTIHSL
                     REQMGIMMQDSFIFSGDIEDNIRYGKLDATLEEIVNASKTVCADEFISKMPDGYQTEV
                     RERGSMLSQGQKQLISFARTLLSDPAILILDEATSSIDVQTEKALQTGLNAMLKGRTS
                     FIIAHRLSTIRNCDKIMYIDNGGIMESGTHDELMAKKGYYYKLYTAQLDEVQKAG"
     gene            complement(1425..2297)
                     /gene="aroE"
                     /locus_tag="PLN77_09500"
     CDS             complement(1425..2297)
                     /gene="aroE"
                     /locus_tag="PLN77_09500"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRM44538.1"
                     /translation="MSKHITGHTGLLCLLGSPVAHSVSPEMHNEACDQLGLDYSYLAF
                     DVPEDKMPQAVEGLRTMGARGWNITMPGKNIMCKLADKVSPASEISGACNTIVNDNGV
                     LTAYTTDGVGFMRAVAENGVDIIGKKMTLLGAGGAATAILVQAALDGVAEINVFNVRD
                     NFFGRAEEIVAKLNERTSCKVTLHDFSDPEVLRASIADSAILVNGTSVGMAPKTENTI
                     ITDTTMFHKDLFVFDVIYNPQETRLLREAKEAGCKTGNGMYMLLYQGAASFKLWTGED
                     MPVEIIKEKYFSGK"
     gene            complement(2499..>3326)
                     /locus_tag="PLN77_09505"
     CDS             complement(2499..>3326)
                     /locus_tag="PLN77_09505"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541936.1"
                     /note="YdiB; quinate/shikimate dehydrogenase from
                     Escherichia coli uses both NAD and NAD(P) to convert
                     quinate and shikimate to 3-dehydroquinate and
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HRM44539.1"
                     /translation="TQSPTTHNLAYDKNGDDVIQLAFEVDNDSLKDAVQSIRALKMLG
                     SNISMPNKTVVHKYLDEVDEAAKLCGAINTVVNLRDENGQVTGKLKGYNTDGMGYWQA
                     LTEEGIDFKGMKMVLIGTGGAATAIAVRGALDGVAEIEIFNIKDKFYSRGEEIVDLIN
                     KNTNCKATMNDLADKDLLKEKMHAADLFCDATGVGMHPLEDLSNIPDPSFFKKDLIVT
                     DTVYAPRETVMMKQAKEAGCEKVFKGMGMMLFQALIAIKLYTGKDTPVDYMKEKLGI"
BASE COUNT          799 a          779 c          864 g          884 t
ORIGIN      
        1 gaatggactg atcaacattg tgctggagtg tttcaacctg ctgttcatcg tcattttcat
       61 gttcctggtc gatgtgcaga tggcgctcgt cgttctctgc ggcgtgccgg tgctgtgcgt
      121 gttcatggtc tggatcaaga ataaacagag acgggcatgg caggcagttt caaataagaa
      181 ctcgaacctg aatgcgtatc tgcaggaaaa tatcgtcgga gcccgcatta cacagatctt
      241 tgcaagagag gatgaaaatg cggagatttt caaagatctt tcacaggact gccgcagaac
      301 gtggaacacg gcggtgcgtt attccaacct ggtatggccg ggcatcgatt cgatttccgt
      361 ctgcgtgcgc gcggcgatct tcttagcagg ccttgtgctg ttcggacagg gcagcaaatc
      421 actcggtacc atcgtggcga tttccagcta cgcatcgtac ttctggcagc cgatcatgaa
      481 ccttggaaat atcttcaaca actttatcaa caatatcgct tacctggagc gtatcttcga
      541 gacaatggac gagccggtaa cggtaaaaga tgcggagaac gccgtaaaaa tgccggagat
      601 ccgcggtgag gtgacgtttg accatgtcaa cttctcgtac gatgaatcga agcagatttt
      661 aaaagacgtt tcgtttacgg taaaaccggg tgagagtgtg gcgcttgtcg gaccgacggg
      721 agccggaaag agtacgattg tcaatctgat ctcccggttc tacaatgtca acggcggccg
      781 cgtcctgatc gacggccagg acatctcaca ggtgacaatt cattcgctgc gtgaacagat
      841 gggtattatg atgcaggaca gctttatttt ctcgggagac attgaggaca acatccgtta
      901 cggaaaactc gatgcgacac tggaggagat tgtcaatgcg agcaaaaccg tgtgtgcgga
      961 cgaattcatc agcaaaatgc cggacggcta ccagacagag gtgcgcgagc gcggttccat
     1021 gctgagccag ggccagaaac agctgatctc gtttgccaga acgctgcttt cggatccggc
     1081 gattctgatt ctcgatgagg caacctcgag tatcgacgtg cagaccgaga aagcactgca
     1141 gacgggcctc aatgcgatgc tgaagggcag aacatcgttc attatcgccc accgtctgtc
     1201 cacgatccgc aactgcgata agatcatgta tatcgacaac ggcggcatca tggagagcgg
     1261 aacgcacgac gaactgatgg caaagaaggg atattactac aaactgtata cggcacagct
     1321 cgacgaagta cagaaagcgg gctgacacca tttcaggaca gcaaaaaggc ttctgccggc
     1381 ggatgaaaat ccggggcaga agccttttga aattgtgtga tattttattt tccggagaag
     1441 tatttctctt tgatgatctc aaccggcata tcctcgccag tccacagttt gaaggaagca
     1501 gctccctggt ataacagcat gtacataccg tttccggtct tgcatccagc ctcttttgcc
     1561 tcacgcagca ggcgggtttc ctgcggattg tagatcacat cgaaaacgaa gaggtctttg
     1621 tggaacatcg ttgtgtcggt gatgatggtg ttctcggttt ttggagccat tccgacggaa
     1681 gttccgttga caagaatggc gctgtctgcg atggatgcgc gaaggacttc cggatcggag
     1741 aagtcgtgca gggtaacctt gcaggaagta cgctcattca gttttgcaac gatttcctcg
     1801 gctcttccga agaaattgtc tctgacgttg aatacattga tctcagccac gccgtcgagg
     1861 gctgcctgta ccaggattgc agtagcagcg ccgccggcac cgagcagagt catctttttg
     1921 ccgatgatgt ctacgccgtt ttctgcaact gctctcatga agccgacacc gtcggtggtg
     1981 taagcggtga gaacgccgtt gtcgttgacg atggtattac atgcgccgga gatctccgaa
     2041 gcaggagaaa ccttgtctgc caatttgcac atgatatttt tgccaggcat ggtgatgttc
     2101 cagcctcttg cacccatggt gcggagtcct tcgactgcct gcggcatttt gtcttccggt
     2161 acatcaaagg caaggtagct ataatcaagg ccgagctggt cgcatgcttc gttgtgcatt
     2221 tccggagaaa cactgtgtgc aaccgggctt ccgagtaaac ataataatcc ggtgtgtcct
     2281 gtgatgtgtt ttgacataat ttgaaatcct ctctctcctc gtatttccgc atgatgataa
     2341 atcgtgctgt agcactttaa actgctacta tatgaagtga attatactcc agatgccgaa
     2401 agaaagcaag aaaaaaagag gaaatgacgc aaagttcatt tcctcttttc gtgttggttt
     2461 ttgattaagg tttaagatgg aaaagattcg cccggaaatc agattccaag tttctctttc
     2521 atgtagtcaa ccggagtatc ttttccggtg tacagcttga ttgcgatgag tgcctggaac
     2581 agcatcatgc ccatgccttt gaatactttc tcacatcctg cttcttttgc ctgtttcatc
     2641 ataacggttt cgcgcggagc gtaaacggta tccgttacga tgagatcttt tttaaagaag
     2701 gaaggatcag gaatgttgga aaggtcttcg agtggatgca ttcctacgcc ggtagcatcg
     2761 cagaacaggt cagctgcgtg cattttttct ttcaggaggt ctttgtctgc cagatcgttc
     2821 atggtagcct tgcagttggt gttcttattg atcagatcaa caatttcctc tccacggctg
     2881 tagaatttgt ctttgatgtt gaagatttca atttctgcaa ctccatccag agcaccgcgc
     2941 actgcaattg cagtagcagc accgccggtt ccgatcagga ccatcttcat tcccttgaaa
     3001 tcgatgccct cttcggtcag tgcctgccag tatcccatac catcggtgtt gtagcctttc
     3061 agttttccgg ttacctgacc gttttcgtcg cggaggttga ctacggtatt gatagcgccg
     3121 cataacttag ctgcttcatc tacttcgtcc agatatttgt ggacaacagt tttgtttggc
     3181 atggaaatat tggaaccgag cattttcagc gcgcggatac tctgcacagc atcttttaaa
     3241 gaatcgttat caacttcgaa agcaagctga atgacgtcat ctccgttttt gtcgtatgca
     3301 aggttgtggg ttgtcggaga ctgggt
//
LOCUS       DAOMUF010000206         3311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8201_length_3311_cov_3.398034, whole genome shotgun sequence.
ACCESSION   DAOMUF010000206 DAOMUF010000000
VERSION     DAOMUF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8201_length_3311_cov_3.398034"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1522)
                     /locus_tag="PLN77_09510"
     CDS             complement(65..1522)
                     /locus_tag="PLN77_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRM44540.1"
                     /translation="MSTENLEHEDQNRTTEPIIDIPGNEAPADPEQEIVSQGVHNYSA
                     AETYVCPTDPAVLQQLEWFRDQKLALMMHFGLYCQPGMVASWALSDKDSDWSRHQVNW
                     TDGETFKKQYFGLNRSFNPVRFQPEEWAQMAEDCGFRYLLLTTKHHDGFCLWDTKYTD
                     YKTTAEDCPFHEHKNADIVKSMFDAFRAKGIGIGAYFSKADWHCPDYWKEDLRKDGLT
                     SRGPSYDPKEYPEVWNRFKKYTKNQVLELCENYGKLDILWFDAGWVSESNGQDIELGK
                     IVEEARKIQPWILSVDRTIGGAYENYVTPELCIPEEPLNVPWESCLTLGVDFTYEYGD
                     HYKSPRELVGMLVNIVAKGGNMALNVSPQPDGRIPVDAWDSLNGFGQWMKTFGDAIYG
                     TRVCAPYQSGTLSFTQKGDSVYVFRLYPTVQESVEAEIFVPYTEAKISEICMVENGET
                     VAFKEVEGGLCITVPAGYRRGSAPIALVFEMKKER"
     gene            1957..2949
                     /locus_tag="PLN77_09515"
     CDS             1957..2949
                     /locus_tag="PLN77_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRM44541.1"
                     /translation="MHMADALVAPAVAATMYVCSGGAAGFSVKKVRLESDPKKIPVMG
                     VMGAFVFAAQMINFTIPGTGSSGHLCGGMMLTALLGPYAAFLTMIGVLLIQCLLFADG
                     GLLALGCNIWNMAFYGCFLGYFLFWRPMMKKGMSRGKIVGASILGCVVTLQLGAFSVA
                     LETLASGITDLPFSVFVATMQPIHLAIGLVEGLITAAVLLFVYEARPEMLSCSEERAK
                     SRFSFKKTIAILGIAALVIGGAVSLAASSNPDGLEWSMERLTGSTELENDGTGAHAAA
                     EEIQEKTALLPDYGFKNSDSTFGTSFSGIAGSAVVLVVCVGACYAFRFFKKKKS"
     gene            2973..>3311
                     /locus_tag="PLN77_09520"
     CDS             2973..>3311
                     /locus_tag="PLN77_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HRM44542.1"
                     /translation="MNKLGNALYEIHHMDMLAAKDQWVNRLHPLVKLVLTIVFLTVTM
                     SFQKYDLTGLLRMGIYPIVLFIVGEISFRDSIRRLRIVLPLVCFVGLFNPIFDRQVIG
                     YIGTAPLTAGM"
BASE COUNT          808 a          785 c          842 g          876 t
ORIGIN      
        1 atcttaataa ttatgaaaag ggctgaacag tcacactgta cagccctttt cgattgtaaa
       61 aaatttaccg ctctttcttc atctcaaaca ccaacgcaat cggtgcggag ccccgtctgt
      121 atcctgccgg gacagttatg caaaggccgc cttcaacttc tttaaacgca acagtttctc
      181 cattttccac catacagatc tcagaaattt tcgcctcggt atacggtacg aaaatttctg
      241 cctctaccga ttcctgtacc gtcggatata accggaatac atagacagaa tcacctttct
      301 gtgtgaacga aagggttccg ctctggtacg gcgcgcagac gcgggttccg tagatggcat
      361 cgccgaaggt tttcatccac tggccaaaac cgttcaggct gtcccaggca tcgaccggga
      421 tgcggccgtc cggctgtggg ctgacgttca gtgccatgtt gccgcctttg gcaacgatgt
      481 tgacaagcat gccgacaagc tcacgcggag atttataatg gtcgccgtat tcgtacgtaa
      541 agtcaacgcc gagggtaagg cagctttccc acggaacgtt cagcggttct tccggaatgc
      601 agagctccgg tgtcacgtag ttttcgtaag caccgccgat tgtacggtca acggagagaa
      661 tccacggctg aatcttacgt gcttcctcaa cgatctttcc aagctcaata tcctggccgt
      721 tgctctcaga gacccagccc gcgtcgaacc agagaatatc cagcttaccg taattctcgc
      781 agagctccag aacctgattt tttgtatatt ttttaaaacg gttccacact tccggatatt
      841 cctttggatc gtaggaaggt ccgcgggatg tcagaccatc ttttctcaga tcttctttcc
      901 agtagtccgg gcagtgccag tcggctttgg aaaaataagc accgataccg ataccttttg
      961 cgcgaaatgc atcaaacatg gattttacaa tatccgcatt tttatgttca tggaacgggc
     1021 agtcctctgc agttgtttta taatctgtat atttggtatc ccagaggcag aatccgtcgt
     1081 gatgctttgt ggtcagaaga agataacgga atccgcagtc ctctgccatc tgcgcccatt
     1141 cctccggctg aaagcggacc ggattaaacg aacgattcag gccgaaatac tgttttttaa
     1201 aggtttcgcc gtccgtccag tttacctggt ggcgggacca gtcggaatcc ttgtcgctta
     1261 atgcccagga agcaaccatg ccgggctggc agtacaatcc aaaatgcatc atcagcgcaa
     1321 gcttctgatc ccggaaccat tcgagctgct gcagaacagc cggatcggtc ggacacacat
     1381 acgtttcagc ggcgctgtag ttgtggacac cctgggaaac aatttcctgt tctggatcag
     1441 caggcgcttc gttgcctggg atatcgataa tcggttccgt tgttctgttt tgatcttcgt
     1501 gttctaagtt ctctgtgctc ataagagagt ctccttctcg tcaagatgaa attcacacca
     1561 atactcgaaa atacagccag aacccgttaa agagccggtt ttggcactaa aataagaaag
     1621 aatcctgccc ggagggcttt tttctgtaga aaaaaccgcg gcgacccgat gacgtgcagg
     1681 cggccgcgga tcggacagga atcctgtcct cgagatagtg tgtgtacgga ctcccgacac
     1741 cggggtatcc gtctgtgttg tctatagaat accatataaa tgttaaaaag tcacccttta
     1801 attttaaaca aaatgttcat aaaaaatcgg taaaagtgaa caaaaaggat cgcggaaaaa
     1861 tctgaaaaaa taacccccac aggggcttgc aggacgaatt ttagcgtagt aaagtaaaaa
     1921 aagaaaaaaa cagaaaacga aaaggaggag aacattatgc atatggctga tgcgctggtt
     1981 gcgcctgcag tggctgcaac aatgtatgtc tgttccggcg gagcagctgg tttttctgta
     2041 aaaaaagtac gcctggagag cgacccgaaa aagatcccag tgatgggagt gatgggtgcg
     2101 tttgtctttg cggcgcagat gattaacttt acaattccag gcacaggctc gagtggtcat
     2161 ctctgcggcg gtatgatgct gaccgcgttg ctcggaccgt atgccgcgtt tttaacaatg
     2221 atcggtgtgc tgctgatcca gtgtctgctg tttgcggacg gcggtcttct ggcgctcggg
     2281 tgcaacatct ggaatatggc gttttatggg tgctttctcg gttatttcct gttctggcgt
     2341 ccgatgatga aaaagggaat gtcgcgcgga aagattgtag gtgcttccat ccttgggtgc
     2401 gtggtgacgc tgcagcttgg tgctttcagt gtggcgctgg aaacacttgc atccggtatt
     2461 acggatctgc cgttttctgt atttgtggca acaatgcagc cgattcatct cgcgattggc
     2521 cttgtagaag gcctgattac agcggccgtt ctgctgttcg tctatgaagc gcgtccggag
     2581 atgctttcgt gcagcgaaga aagggcaaag agccgatttt ctttcaagaa gacaattgcg
     2641 atcttaggaa tcgcagcact ggtgatcggc ggagccgttt ctcttgcggc atcctccaat
     2701 ccggatggac ttgagtggtc tatggagcgt ctgactggtt cgacggaact ggaaaatgac
     2761 ggaaccggcg cacatgcagc ggcggaagaa attcaggaaa aaacggcact tctgccggac
     2821 tatgggttta aaaattccga ttccacattt ggaaccagct tttccggaat tgcgggatcc
     2881 gcagtcgttc ttgttgtctg tgtcggtgca tgctatgcat tccgtttctt taaaaagaaa
     2941 aagtcataag ctgcacggaa caggagaaag aaatgaataa acttggcaat gcactatatg
     3001 aaatccatca tatggatatg cttgcggcga aggaccagtg ggtgaacagg cttcatccac
     3061 tggttaaact tgttttaacg attgtttttc tgaccgtaac tatgtccttt caaaaatacg
     3121 atctgaccgg cctgctgcgc atgggaattt atccgattgt actctttatc gtcggcgaaa
     3181 tttcattccg cgacagtatc cggcggcttc ggattgtcct gccgcttgtc tgctttgtgg
     3241 ggctgttcaa tccgattttt gaccgtcagg taatcgggta tattggtact gctccgctga
     3301 cagcagggat g
//
LOCUS       DAOMUF010000207         3265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8346_length_3265_cov_3.455763, whole genome shotgun sequence.
ACCESSION   DAOMUF010000207 DAOMUF010000000
VERSION     DAOMUF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8346_length_3265_cov_3.455763"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1850
                     /locus_tag="PLN77_09525"
     CDS             135..1850
                     /locus_tag="PLN77_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44543.1"
                     /translation="MKKKQAWKVMGAVLAGTLVFSDAIPLRASASAGTEKSAVQNETV
                     SGTENGTETKTKRAAVYVNLKTGDDTADGTTAETAVKTVEKAQELLAAAAAELQADGK
                     TTEKNTTADTSKTAAEEAAEKYLVFVGMDEKELETYRKKQQKTDFWGRVTDTVFAADE
                     IVVTEAGYEAVLAAEKAKETVSETPTLAPGESPAPDAAEDTVTPTPGPTETPDAEPTA
                     EPTVTPDAEPTVEPTETPDAEPTVTPAEEPTATPTPDVDNGENTEDTPTAAPTPDAEP
                     TVTPEVTPTTAPGENTDDSENPENPETPAEDPTVTPAPDDTAKDDPAADDTNQDDTNQ
                     DNQNGTGNDASSDQWKQDENLDDLVKDEIQKENETVKNFDEISNPSGTDSTEDGYLIS
                     DVSLLIQPMAVSLSGSVVSEEQTETAEAETADEGEHELLAAAEQKSNLSAGEMTASAA
                     DDVSTLRAAGTILVGPDNYGTNKTSTPTPTATPSKSSSNNSSSNSNTGSTKIPQKKTQ
                     TTASIRSIPVNTGDETMVLPLAISSALSAAVMFCMAFLQMQAKRAKSMAAWLEFKKNH
                     PFDEE"
     gene            1912..2304
                     /locus_tag="PLN77_09530"
     CDS             1912..2304
                     /locus_tag="PLN77_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HRM44544.1"
                     /translation="MEEKKTWAVGAGSGIGWLKALLVSYLISVLLLLLLSFLLWKFSW
                     KESWVQAGIVVIYLASSFSGGRMAGNMAGKRRFLWGICFGIVYFAILIIVSAVFLPGG
                     FAGAGRLLWAAAVCAVGGMAGGMTAHGR"
     gene            2438..2578
                     /gene="scfA"
                     /locus_tag="PLN77_09535"
     CDS             2438..2578
                     /gene="scfA"
                     /locus_tag="PLN77_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HRM44545.1"
                     /translation="MKHIKTLNTKSLQNTVKKGGCGECQTSCQSACKTSCTVGNQSCE
                     HK"
     gene            2695..>3265
                     /locus_tag="PLN77_09540"
     CDS             2695..>3265
                     /locus_tag="PLN77_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HRM44546.1"
                     /translation="MIHLYKSNGFNMVLDVNSGSVHVVDDCVYDVLSCMNEGKNAEET
                     AEALSSKYAEQDITEAREEIQELADNGMLFTKDIYENAIDQFSNRPTVVKALCLHIAH
                     DCNLACRYCFAEEGEYHGRRALMSLEVGKKALDFLIANSGSRRNLEVDFFGGEPLMNW
                     DVVKQLVAYGREQEKLHNKNFRFTLTTNGV"
BASE COUNT         1028 a          771 c          869 g          597 t
ORIGIN      
        1 acgaatcccg cgtttcaagg cgacttcagc cttgcgcggg attctttctt gtgctataat
       61 gctgtaatgc gaaattatga cacaaaaaga cagatttttt taggatagat caagagaaca
      121 taaggagaaa cgcaatgaag aaaaaacagg catggaaagt gatgggtgct gtccttgcgg
      181 gcacactggt attttccgat gcaattccgc tccgtgccag cgcatcggca ggaacggaaa
      241 aaagcgcagt acagaacgaa actgtctccg gaacggagaa cggtacagag acaaagacaa
      301 aacgggcagc cgtttatgtg aatctgaaaa ccggcgatga tacggcagac ggtacaacgg
      361 cagagacagc agtaaaaacc gtggaaaaag cacaggagct tcttgcagct gcagcggcag
      421 aactgcaggc ggacgggaaa acaaccgaaa aaaacactac tgcagatacc agcaaaacgg
      481 cagcggagga agcagcagaa aaatatctgg tattcgtcgg aatggatgaa aaagagctgg
      541 aaacgtatcg aaagaaacag cagaaaacag acttctgggg aagagtaaca gataccgttt
      601 ttgcggcaga tgaaatcgtg gtgacagaag ccggatatga ggctgttctg gcagcagaaa
      661 aagcaaaaga aacggtttcg gaaacaccaa cgcttgcgcc aggcgagtct ccggcaccgg
      721 atgcggcaga agataccgta acaccaactc cgggtccgac ggaaacgccg gatgcagagc
      781 cgaccgcaga accaacggtg accccggacg cagagccaac ggtagaaccg acggaaacgc
      841 cggacgcaga gccgaccgta acgccggcgg aggaaccaac ggcaactccg acaccggatg
      901 tggacaacgg agaaaataca gaggatacac caacggcagc gccgacaccg gatgcggaac
      961 cgaccgtaac gccggaagta actccgacga cagcaccggg cgagaatacc gatgacagtg
     1021 aaaatccgga gaatccggaa actccggcag aagatccaac cgtgacacct gccccggatg
     1081 atacagcaaa agacgatcct gcagcagatg atacaaacca ggacgataca aaccaggata
     1141 accagaacgg aacgggaaat gatgcatcat cggaccagtg gaaacaggat gaaaatctgg
     1201 atgaccttgt aaaagatgaa attcagaaag aaaacgaaac cgtaaaaaat ttcgatgaaa
     1261 tttccaatcc atccggtacg gacagcacag aagacggcta tctgatctcc gatgtctctc
     1321 ttttgatcca gccgatggca gtaagtctgt ccggaagcgt tgtatcagag gagcagacgg
     1381 aaacagcaga agctgagacg gcggacgaag gcgagcatga actgcttgca gctgcagagc
     1441 agaaatccaa cctgagtgcc ggtgaaatga cagcaagtgc agccgatgac gtaagtacgc
     1501 tgcgtgccgc aggaacgatc cttgtaggac cggacaacta cggaacgaat aagacatcca
     1561 ctccgactcc gacagccaca ccgtcgaaga gcagcagtaa caacagctct tccaattcca
     1621 ataccggaag cacaaagatt ccgcagaaga agacacagac cactgcaagc atccgcagca
     1681 tcccggtcaa caccggcgat gagacgatgg tgcttccgct ggcgatcagc tccgcacttt
     1741 ctgcagccgt tatgttctgc atggcattcc ttcagatgca ggcgaaacgc gcaaagagca
     1801 tggccgcatg gctggaattc aaaaagaatc atccgtttga cgaagaataa agaaacaggc
     1861 atatccgaaa aagccattcc atacatttac agtatgggag ggctttttct tatggaagaa
     1921 aagaaaacat gggcggtggg tgcgggcagc gggatcggct ggctgaaggc tcttctcgtt
     1981 tcctatctga tctcggtgct tcttcttttg ctgctgtctt tcctgctctg gaaattttcc
     2041 tggaaggaaa gctgggtgca ggcgggcatt gtggtgatct atctggcatc aagcttttcc
     2101 ggcggaagaa tggcaggaaa catggcggga aaacggcggt ttctctgggg catctgcttt
     2161 ggaatcgtgt attttgccat tctgatcatc gtctccgcag tcttcctgcc gggcggattt
     2221 gccggagccg gaagacttct gtgggcggca gcagtctgtg ccgtcggcgg aatggcaggc
     2281 ggaatgacgg cacacggcag ataaaagcgg acaaaacaga acattttttc aaaaaacaga
     2341 cttttaaatt tgaaaaacct atgctataat gggaagagct aatggccgaa gactataaaa
     2401 aagtcgcgcg tcaactacaa tgaaggagga atgtgccatg aaacacatca aaactttaaa
     2461 tacaaaaagc ttacagaaca ctgtaaaaaa aggtggatgc ggtgagtgcc agacatcctg
     2521 ccagtccgca tgtaagactt cttgcaccgt aggcaaccag agctgcgagc ataagtaaaa
     2581 gtaatccata aggacgagta aaaggtgttc ccagaaccgg tttcgccgga tttgggaaca
     2641 ttctttttgc caggcacatc aaaaaaagag agagttatag aaaggaatac cacagtgata
     2701 catttatata aaagcaatgg cttcaacatg gtgctggacg tcaacagcgg ctccgtccat
     2761 gttgtggatg actgcgtata cgatgtcctt tcctgcatga acgaaggaaa aaatgcagaa
     2821 gagacagcag aggcgcttag cagtaaatac gccgagcagg acatcaccga agccagagaa
     2881 gaaattcagg aacttgcaga caacggcatg ctctttacca aagatatcta cgaaaacgca
     2941 atcgatcagt tttccaaccg tccgaccgtc gtaaaagcac tctgcctcca tatcgcacac
     3001 gactgcaatc ttgcctgtcg ttactgcttc gcagaagagg gcgagtacca cggaagacgt
     3061 gcgctgatgt ccctggaagt cggcaaaaaa gcgcttgatt tcctgatcgc aaactccggc
     3121 agcagaagaa acctcgaggt agatttcttc ggcggtgaac cgctgatgaa ctgggatgtt
     3181 gtaaaacagc tcgtagccta cggaagagaa caggagaagt tacataacaa gaacttccgt
     3241 ttcaccctga ccaccaacgg tgttc
//
LOCUS       DAOMUF010000208         3235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8436_length_3235_cov_4.101258, whole genome shotgun sequence.
ACCESSION   DAOMUF010000208 DAOMUF010000000
VERSION     DAOMUF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8436_length_3235_cov_4.101258"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1505)
                     /locus_tag="PLN77_09545"
     CDS             complement(108..1505)
                     /locus_tag="PLN77_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRM44547.1"
                     /translation="MLQLIETINNAVNNFIWGVPAMICIFGVGLYLSLRTRFLQIRKF
                     PYAIRTTLGRMFRKRDASDGAITPFQAVCTALAATVGTGNIAGVAGAIAIGGPGAVFW
                     MWVSALLGMCTKFAEVTLAVFYHEKNANGELVGGPMYYIKNGLSKKWHFLAYLFAAFG
                     VLTVFGTGNATQVNTITTAVNSALMNYHVISKDAIPTSNLIQGIIIAVLVALILLGGV
                     KRIAQVTEKLVPFMALFYIVLAIGVILLNLNRIPSVFVSILEGAFRPSAVTGGIVGSM
                     LMSVKKGVSRGIFSNEAGLGTGSIAHACADTKKPVKQGMFGIFEVFTDTIVICTLTAL
                     VILCSGTSITYGQAAGAELTISGFTLTYGNWVSLLTAFAMCCFAFSTILGWGLYGARC
                     IEFLFSERITKYFMAAYALVAILGATANLGLMWSIAETFNGLMAIPNLIALFLLSGKV
                     VELTKEYFSGEGKTR"
     assembly_gap    1920..1929
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1930..2825
                     /locus_tag="PLN77_09550"
     CDS             <1930..2825
                     /locus_tag="PLN77_09550"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRM44548.1"
                     /translation="INLDKLEKGGSLCQCFSLFTFLKGLKDETPFEHVRKLADLWKSE
                     VERYPDRICQIKTYADLMQVQKEGKIGAVMTVEEGGVYEGSIEKLHTLYDLGVRISTL
                     TWNFPNELGFPNPAQEEGKPYLPDLENGLTSTGIAFVEEMERLGIVIDLSHLNDAGIR
                     DVFAHTRGPVIASHSNARGACFHLRNLSDEMIRQLAERGGVTGINFCPSFLREEEPAA
                     KKASTMDMVRHMKYLRNVGGIDIIGLGTDFDGFSGETDVPDTAHMQHLADVMSHDGFT
                     DEEIEKVFSKNVLRVFRECWK"
     gene            complement(2828..>3235)
                     /locus_tag="PLN77_09555"
     CDS             complement(2828..>3235)
                     /locus_tag="PLN77_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44549.1"
                     /translation="KEERKKRVLAMAERVGLSAEHLKRRPHELSGGQRQRVSIAAALI
                     QGSRLILADEPVSALDVTIQKQIMELMVQLQEELQLSILFISHDLNVIYQMCDRVLVM
                     KEGRIVENAETEELFAHPQNDYTKELLAASPEL"
BASE COUNT          849 a          754 c          866 g          756 t
ORIGIN      
        1 tataaacttt taacagtgta aggggtagta acctgattgt caagggaaaa ggcgaattta
       61 catgattttc ttacagaaga agggaatgcg ccgcgagaaa aggaatttta ccgggttttg
      121 ccttctccgg aaaaatattc ctttgtaagt tccacaacct tgcccgaaag caggaagagc
      181 gcaatcaggt ttgggattgc catgaggccg ttgaaggtct cggcaatgct ccacatcagg
      241 ccgagatttg cggtggcgcc aaggatggca accagcgcat aggcggccat aaaatatttt
      301 gtgattcttt cggagaacaa aaactcgatg cagcgtgcac cgtaaagtcc ccatccgaga
      361 atcgtggaaa aggcgaagca gcacatcgcg aacgcggtga gcagcgaaac ccagtttccg
      421 tatgtaaggg taaaaccgct gatggtcagc tctgcgccgg cagcctgtcc gtaggtgatg
      481 gaagtaccgc tgcagagaat aacaagtgca gttaaggtac aaatcacgat cgtatcagta
      541 aatacctcaa agataccgaa cataccctgt ttgaccggct tttttgtgtc ggcacaggcg
      601 tgcgcgatcg aaccggtacc aagacctgcc tcgtttgaga aaataccgcg ggacacacct
      661 tttttcacac tcatgagcat gcttccgacg ataccgcctg taacagcaga gggacggaag
      721 gcgccctcaa ggatcgatac gaaaacagac ggaatccggt tgagattcag caaaattaca
      781 ccgatggcaa ggacaatata aaacagagcc ataaacggaa cgagcttttc tgttacctgt
      841 gcgatccgtt tgacaccgcc gagcaggatc agcgctacga gtaccgcaat gatgataccc
      901 tgaatcaggt tggaggtcgg gatcgcgtcc tttgagatca catggtaatt catcagtgcg
      961 gagttgactg cggttgtgat ggtgttgacc tgcgttgcat ttccggttcc gaacacagtc
     1021 agtacaccga aagcagcgaa aagataggcc agaaaatgcc attttttgct gagaccgttt
     1081 ttgatgtaat acatcggtcc gccgacgagt tctccgttgg cgtttttctc gtgatagaaa
     1141 acagccagtg ttacctcggc aaatttcgta cacatgccga gcagggcgga aacccacatc
     1201 cagaacacgg caccgggtcc gccgatagcg atagcgccgg caacaccggc aatattaccg
     1261 gtaccgaccg tggcggcaag tgctgtacag accgcctgga acggtgtaat tgcgccatcg
     1321 gaagcatctc gtttgcggaa cattcttccg agcgttgtgc ggatcgcata cgggaatttt
     1381 cgaatctgga gaaaacgggt gcgcagactc aggtaaaggc cgacaccgaa aatgcagatc
     1441 atagccggaa caccccagat aaaattgttc acggcattgt tgatggtttc aatcagttgg
     1501 agcatagata cctctttctt tttcataagc agaagcactt tcttggaaga tcagtgcttt
     1561 cataaagtaa cacaatgcaa aaagtatatc atctcagacg agatttgcaa gtttttttcg
     1621 agaaaaaacg cggaccccca attttttgcg attccggaaa gatatatttt ctctagcctt
     1681 atgggtttac catgagccag gcggtttttt acgttttctt ccagtttttg tgaaggaatg
     1741 cagcgaagta tgatagaata gagaaaaaac agaaaagagg attattgcca tgtttcaggt
     1801 aattgatatg cactgcgaca ccatcccggc gctgcagtgg aaggaaaaac aggagcatct
     1861 tgcggatgcg gatcttcaga tcaatctgga taagttcggt cagaagctgt cgattctgan
     1921 nnnnnnnnna gatcaatctg gataagctgg aaaagggcgg cagcctctgc cagtgctttt
     1981 ctttgtttac gttcctgaag ggactgaaag acgaaacacc gtttgagcat gtgagaaagc
     2041 tggctgatct ctggaaatca gaggtggaac ggtatccgga ccgcatttgt cagatcaaaa
     2101 cgtatgcgga tctgatgcag gtacagaagg aaggaaaaat aggtgcggtc atgaccgtcg
     2161 aagagggtgg tgtttacgaa ggcagtatcg aaaaactgca cacactgtac gatctcggtg
     2221 tgcggatttc cacgctgacc tggaactttc cgaatgagct tggattcccg aacccggcgc
     2281 aggaagaggg aaagccatat cttccggatc ttgaaaacgg gctgacatcg accggtattg
     2341 cctttgtcga ggagatggag cggctcggca tagtgatcga tttatcacac ttaaatgatg
     2401 ccggaatccg cgacgttttt gcgcatacga gaggtccggt cattgcgtcc cattccaatg
     2461 cgcgcggcgc gtgcttccat ctgagaaacc tttcggatga aatgattcgt cagctcgcag
     2521 agcgtggcgg cgtcaccggt atcaatttct gtccgtcgtt tctgcgcgag gaggagccgg
     2581 cagcgaaaaa ggcatcgacg atggatatgg tacgccatat gaaatatctg cgcaacgtcg
     2641 gcggtatcga tatcatcggc cttggaaccg attttgacgg tttcagcggt gaaacggatg
     2701 tgccggacac cgcgcatatg cagcatctcg cagatgtgat gagccacgac ggtttcaccg
     2761 atgaggagat cgagaaggtc ttttccaaga atgtacttcg ggtgtttaga gaatgctgga
     2821 aataaaacta cagctccggt gatgccgcca gaagctcctt tgtataatca ttctgcggat
     2881 gcgcaaacag ttcttccgtc tccgcgttct ccacaatccg gccttctttc atcacgagaa
     2941 cccggtcgca catctgatag atcacgttga ggtcatgcga gataaagaga atcgagagct
     3001 gaagctcctc ctgaagctga accatcagct ccatgatctg cttctgaatc gtcacatcga
     3061 gagcggaaac cggttcgtcg gcaaggatca ggcgtgagcc ctggatcagt gcggccgcga
     3121 tgctgactct ctggcgctgg ccgccggaga gctcatgcgg gcggcgtttc aggtgctctg
     3181 cggagaggcc gactctttcg gccatcgcaa gaacccgttt ttttcgttct tcttt
//
LOCUS       DAOMUF010000209         3224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8481_length_3224_cov_2.479015, whole genome shotgun sequence.
ACCESSION   DAOMUF010000209 DAOMUF010000000
VERSION     DAOMUF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8481_length_3224_cov_2.479015"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLN77_09560"
     CDS             complement(<1..364)
                     /locus_tag="PLN77_09560"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM44550.1"
                     /translation="MHSIFWFRTRQWVLSRVLHLTEHKKQTLLAAPDAVLLLPALFRK
                     RRISRILLVTTPGFVRRGTLTSLLDGCKKENISVTIFSEIAPDPQIESVEQGFRLYQQ
                     IQAQAILAIGGGSVLDAAK"
     gene            581..>1381
                     /locus_tag="PLN77_09565"
     CDS             581..>1381
                     /locus_tag="PLN77_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRM44551.1"
                     /translation="METKRDYYEVLGVSRNADEKEIKRAYRKLAKKYHPDTNAGNPDA
                     EKKFKEVTEAYSVLSDPEKKKMYDQFGFAAFDQGAGAYSQQGGGNPYGSAYGNPFGGS
                     GNNGGYHEFHFEGNADDMFGDFFDQMFHGKSRGFGSERYTRSGSSRHPFEDAFSGRGM
                     AEDLDLHAEVSIRLEEAASGCEKQITLRNPDGTTNTLLVKIPAGIESGKSIRLRGKGH
                     TGSNGRRGDLLLKVNVEEKKGFERRGMDIYTTIQIPYTTAVFGGEARVA"
     assembly_gap    1382..1413
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1414..1613
                     /locus_tag="PLN77_09570"
     CDS             <1414..1613
                     /locus_tag="PLN77_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRM44552.1"
                     /translation="LYGDVVCKIKEGTQSGSKLRLRGKGMVSMKDANVHGDHYATIEI
                     AVPRSLNRTARQKLMEYKEAC"
     gene            1844..2431
                     /locus_tag="PLN77_09575"
     CDS             1844..2431
                     /locus_tag="PLN77_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRM44553.1"
                     /translation="MNTLKAEKRSLDVKAKRLRREGYVVGNVFGREMKESIPVKMVKR
                     DVEKLLKTDHKGSQIMLDVDGAAYDVLIKNVDYNPVAGQIDSIEFQALVSNEKVHSVA
                     EIVLVNHEKIAEGVLQEDLNEVAYMAYPASLVDQVMVDVGEMKIGDVIRVKDLSLAAD
                     PDITIKTNPESIVVSLMASHNVEAPAEDTEEAEEK"
     gene            complement(2512..3126)
                     /locus_tag="PLN77_09580"
     CDS             complement(2512..3126)
                     /locus_tag="PLN77_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM44554.1"
                     /translation="MHHRRSAPGRILSRIASIVGTAALAALAAGGTAAYAADAYPSKP
                     IRLVVPYPAGGGTDIIAMIIKKYSTMNISTALLVTDLIVVILAFLIFDTLTGLCSVLG
                     LMAKTLLIDKTIERMKLNKFFTIISNNPQPICDFITKDLNHSATLYDAVGAYSDQQRT
                     VILTVVDVKQAVYLQRFIHDTEPTAFIAITKSSEIIGKGFMSYI"
BASE COUNT          936 a          699 c          878 g          679 t
ORIGIN      
        1 gctttgctgc atcgagaacg gatccgccgc cgatggcgag gatagcctgc gcctgaatct
       61 gctgataaag acggaaaccc tgttccacgc tctcaatctg cggatccggg gcgatttcgg
      121 agaaaatggt gacggaaatg ttttcttttt tacagccatc cagcagagac gtcagtgttc
      181 cgcggcggac aaagccgggg gtagtcacca ggagaatccg ggagatccgg cgtttccgga
      241 ataacgcggg gagcagcaga acggcatccg gtgctgcaag gagtgtctgt ttcttatgct
      301 ctgtcagatg aagaacgcgg ctcaacaccc actgccgggt gcggaaccag aaaatggaat
      361 gcatcaatca agtacctcca taaaaaatcg tgagaatcgt atatctctta ttgtaacagt
      421 gcacggcagg tttttcaatt cgtataggaa ttttgaatct tccgtgaaaa gaattgacag
      481 tgtgaaaaat gcgtgttata gtacgtatag aacagataaa aagagccagg aattgttcac
      541 ctgggaaatc aggaaaaggc ttgactggag gtgcctgatg atggaaacaa agagagatta
      601 ttatgaagta ttaggcgtca gccggaatgc agacgagaaa gaaatcaagc gtgcataccg
      661 gaaactggcg aaaaaatatc atcccgatac gaatgcggga aacccggatg cggaaaagaa
      721 atttaaggaa gtgacagaag cgtattctgt cttaagcgat ccggaaaaga agaaaatgta
      781 cgatcagttc ggattcgcgg cgttcgacca gggagccggc gcgtacagcc agcagggcgg
      841 tggaaatccg tacggcagtg cgtatggaaa cccgttcggc ggaagcggaa acaacggcgg
      901 ctatcacgaa tttcactttg aaggaaacgc ggacgatatg ttcggcgatt tctttgatca
      961 gatgtttcat ggcaaaagcc gtggattcgg aagcgagcgg tatacgcgtt cgggaagcag
     1021 cagacatccg tttgaggatg cattttccgg ccgcggtatg gcagaggatc tcgatctgca
     1081 cgctgaggtt tcgatccgcc tggaggaagc ggcatcgggc tgtgaaaaac agattaccct
     1141 gcggaacccg gatggaacga ccaatacgct gctggttaaa atcccggccg gtatcgagag
     1201 cggaaagagc atccgtctgc gcggcaaagg ccatacagga tcaaacggcc gccgcggcga
     1261 tctgcttttg aaggtcaatg tagaagaaaa gaaaggtttt gaacggcgcg gaatggacat
     1321 ctacacgacg attcagattc catacacaac ggcagtcttt ggcggcgaag cgcgtgtcgc
     1381 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncactgta cggagatgtg gtctgcaaaa
     1441 tcaaggaagg aacacagtcc ggcagtaagc tccgtctgcg cggcaagggc atggtatcga
     1501 tgaaagatgc gaacgtgcac ggtgatcatt atgcgacgat tgagattgct gtaccgcgca
     1561 gtctgaaccg cacagcacgc cagaaactga tggaatataa ggaagcgtgc tgaaaatgtg
     1621 aagaaactgt gaaatcggaa aatccgggtt gacaaatcgt gtttgtggta ttatattaca
     1681 cctagaacaa agaaaaggaa tcaacaagaa accaattctt ccttgcatgt tcccaaacat
     1741 cctgttccaa gaaacttatt catagccaca aggcaaagca aacttaacac ccttcacaag
     1801 ataagaagcc tgctaagaat cagatttttg gaggaaaagg taaatgaata ctttaaaagc
     1861 tgaaaagaga agtttggatg taaaagcaaa aagactcaga agagaaggat acgttgtcgg
     1921 caatgtattc ggcagagaga tgaaagaatc cattccagtt aagatggtaa aaagagatgt
     1981 cgagaaactg ctgaagacag accacaaagg cagccagatt atgctggatg ttgacggagc
     2041 cgcatacgat gtcctgatca aaaatgttga ttataacccg gtagccggac agatcgattc
     2101 gatcgagttc caggcactgg tcagcaacga gaaagttcat tccgttgcag agattgtcct
     2161 tgtaaaccat gaaaagatcg cagaaggcgt tctgcaggag gatctgaacg aggttgccta
     2221 catggcatac ccggcatccc tggtcgatca ggtaatggtc gatgtcggag agatgaagat
     2281 cggtgatgtc atccgcgtca aagatctgtc cctggccgct gacccggata tcaccataaa
     2341 gaccaatccg gaatccattg tcgtaagtct gatggcttcc cacaacgtag aagcaccggc
     2401 agaagatacc gaagaagcag aagagaaata aacagaataa gcatatacaa agaaatccga
     2461 aggcgtcaaa aaccttcgga ttttttgcat gaaaaatatg gtttgaatcg atcagatata
     2521 actcatgaag ccttttccga taatttcgct gctctttgtg atcgcaataa acgcggtcgg
     2581 ctcggtatca tgaataaacc gctgaagata gacggcctgc ttgacatcca caaccgtgag
     2641 aatgactgtc cgctgctggt cggagtaagc gccgacggcg tcgtaaaggg ttgcgctgtg
     2701 attgagatcc tttgtgatga aatcgcagat cggctgcgga ttgttggaga taatcgtaaa
     2761 aaatttattc agcttcatcc gctcaatcgt tttgtcaatc agcagtgttt tcgccatcag
     2821 accgagaacc gaacaaagtc cggttaaggt atcgaaaatc aggaaagcaa ggatgacaac
     2881 gatgaggtcg gtcaccagca gggcagtgga aatattcatt gtagaatatt ttttaataat
     2941 catggcgatg atgtccgtgc cgccccctgc cggatagggc accaccagcc ggatcggctt
     3001 gcttggatag gcatcggccg cgtaggcggc ggtgccccca gcggccagcg ccgccagcgc
     3061 ggcagtgccc acgatgctgg caatacggct cagaatacgg cccggggccg aacgacggtg
     3121 gtgcatgtct agtctcctga tcaatttttt tgggtgacgg gccaacccag gcgcgcaacg
     3181 cctgaagggg ctacaagccc cttgcgatga tttccttcat gatt
//
LOCUS       DAOMUF010000210         3164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8709_length_3164_cov_1.726600, whole genome shotgun sequence.
ACCESSION   DAOMUF010000210 DAOMUF010000000
VERSION     DAOMUF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8709_length_3164_cov_1.726600"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3164)
                     /locus_tag="PLN77_09585"
     CDS             complement(<1..>3164)
                     /locus_tag="PLN77_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRM44555.1"
                     /translation="SSKNKNDETQLQRELDELFPQEFVDFLAQTGMDYESRRVKVNSG
                     KNFADGILWMSFTDYPLLLLVENKKLLSLLKKRCAHMMVNDVIRFPLPMACKGAEPLL
                     VMSDARFPEKAKQLSHAWMFGVLASSPVAKLEITVIDPENRGTSVGPFFDARKRLPEL
                     FGEKICFTAEDIATQLALLNEEIETTLMDKLGTEYDSIFDYSREHPEETLRVRCLVMY
                     DFPRNISDSILTDVRNILRNGSRCGIYTMIICPPPQPGTRQESYRQMLQTIQPLTAVI
                     KQDQQSFSERGLPLLYHPMPDKAGFDRFFSKYMLIFEGMQNRGIAFSPFIRKLVEAKD
                     DAELDVSIQNLTEWKQISQEPFDQQSASTTFPELFCMGQIAYPAEIFDESIGYKKIVR
                     AFRAETPGHSIDMSRVELPMMFNLHHPLNLEVIGAEAQHDNMQSAAYRVIWGFLRKCP
                     ASKVRFCIFDPKEGGGSVRMLSNFVNKMPDSYKRAVTQMSRTEELLSCLKELEGQTLD
                     FIRDRPDYDDLLDYNAHNPRRTEAITLLMLYDFPLNADARCLELLSSVMQKGNRCGIY
                     VVLCRNTAVEVASSYDHIDEKLAELEKNCVQIECKENGFALLPYHLSVRLIEKPDAGQ
                     LEKFAVEYHKAVEKLSVQSIHFEEILPPEPFQGSTAKVLKLPMGIGDGDSVVSMVFGE
                     GTSHHGLIGGGTGGGKSTLLHTLIMSSMMNYSPEQLNLYLMDFKGGTEFKIYESERLP
                     HIKLLALDALQEFGESILENLVQEMANRSDIFKRSGGYTKLEDYVTNTGNSMPRILVI
                     MDEFQILFDSGTNRKVAERCANLAKKIVTEGRSYGVHLLMATQSTKIISTLTLDRGTI
                     EQMRIRVGLKCGEDDTRYLFGDTHCSDAMKKMEGPRGTAVLNEDYTENNPNVGLRVAF
                     CNDETKKYYLKQISEKFADSPCTMQVFEGSRTVPMLDYLAARSIGIADTSTVTVHMGE
                     KIKVDDPFELTFDRKKQHNVLICGSNDELMDRTVNLFLLSAALNRRACVYCVDGDSIV
                     GDDRCGSFYQSLEHGAAELHTAHT"
BASE COUNT          758 a          867 c          730 g          809 t
ORIGIN      
        1 gggtatgcgc agtgtgcagt tctgctgcac catgctcgag gctttgataa aaacttccgc
       61 agcggtcatc tcccacgatg ctgtcgccat ccacacagta cacacacgcc ctgcggttca
      121 gcgcagccga gagcaggaac agattgaccg tccgatccat cagttcatca ttggaaccac
      181 agatcaggac attatgctgt ttcttgcggt caaacgtcag ttcaaacggg tcatccacct
      241 taattttttc gcccatatgc acagtgaccg tgctggtatc ggcaataccg atcgaccgtg
      301 cagcaagata gtccagcatc ggcacagtgc gggagccctc gaacacctgc atcgtgcagg
      361 gagaatccgc aaacttctca ctgatctgtt tcaggtaata ctttttggtt tcatcattgc
      421 agaaagcgac ccgcaggcca acgttcggat tgttctctgt gtagtcttcg ttcagcacag
      481 cggttccgcg gggtccttcc atttttttca ttgcatccga gcaatgggta tcaccaaaca
      541 gataacgagt atcatcttcg ccacatttaa ggccgacacg gatacgcatc tgctcgatcg
      601 taccacgatc cagcgtcagg gtcgaaatga ttttggtgga ttgagtcgcc atcagaagat
      661 gcacgccgta ggaacgtcct tccgtaacga tcttcttcgc cagatttgcg caccgctctg
      721 ccaccttgcg gtttgttccg ctgtcaaaca aaatctggaa ctcatccatg atgacaagga
      781 tacggggcat gctatttccg gtattggtaa catagtcttc cagctttgta tatccaccgg
      841 atctcttgaa tatatccgag cgattcgcca tctcctggac cagattttcc aagatgctct
      901 cgccgaactc ctgcagtgca tccagcgcca gcagcttgat gtgcggcaac cgctccgatt
      961 catagatttt gaactcggta ccgcccttga aatccatcag atacaaattg agctgttccg
     1021 gcgaatagtt catcatactg ctcataatca gcgtatgcag cagggtcgat ttgccgccac
     1081 ccgtaccacc gccgatcagg ccatgatgcg aagtgccctc gccaaacacc atgctcacga
     1141 ccgaatcgcc atcgccgatg cccatcggca gcttcagtac cttggctgtg ctgccctgga
     1201 acggttccgg cgggaggatt tcttcaaaat ggatgctctg aacgctcagt ttttcgactg
     1261 ccttgtgata ttctaccgca aatttttcca actgtccggc atcgggtttt tcgatcaaac
     1321 ggaccgagag atgatacggc agcagagcaa agccgttttc tttacattcg atctggacac
     1381 aattcttttc cagttcggcc agtttttcgt ctatgtggtc atagctgctt gccacctcga
     1441 ccgcagtatt ccgacacaga acgacataga tgccgcacct gtttcctttc tgcataacag
     1501 aggacagcag ttccagacag cgagcgtccg cattcaacgg gaaatcatac agcatgagca
     1561 atgtaatcgc ttctgtacgt cttggattgt gcgcattata atccagcaaa tcatcataat
     1621 cagggcggtc acggataaaa tcaagcgtct gtccttccag ctctttcaga cagctcagca
     1681 attcttctgt ccgggacatc tgcgtgacag cccttttgta ggaatccggc attttattga
     1741 cgaagttgga caacatacgg acacttccgc cgccctcttt cggatcaaag atacagaagc
     1801 gcactttgga tgccgggcac ttccgaagga agccccagat tacgcgatat gctgcactct
     1861 gcatattatc gtgctgcgcc tcagctccga ttacctccag attgagtgga tgatggagat
     1921 taaacatcat cggcaattcc acacggctca tatcaataga gtgacccggt gtttccgcgc
     1981 ggaaagcacg cacgattttt ttatagccga tgctttcatc aaagatttcg gccggataag
     2041 cgatctggcc catgcagaac aactccggga aagttgtact tgccgactgt tgatcaaatg
     2101 gctcctgaga tatctgtttc cattcagtaa ggttctgaat ggaaacatcc agctctgcat
     2161 catctttcgc ttcgaccagc ttgcggataa acggcgagaa cgcaatgccc cggttctgca
     2221 ttccctcaaa gataagcata tacttactga agaagcggtc gaaaccggct ttatccggca
     2281 tcggatgata aagcaacggc aagccgcgct ccgaaaagct ctgctgatcc tgcttgatga
     2341 ccgctgttag aggctgtatc gtctgcagca tctgccggta gctttcctga cgtgtgcctg
     2401 gctgcggcgg cgggcaaatg atcattgtat aaatgccgca ccggctgcca ttacgcagaa
     2461 tattgcgcac atccgtaaga atcgaatcgc tgatattgcg agggaaatca tacatcacga
     2521 ggcagcggac tcgcagggtc tcttccgggt gttcccttga atagtcaaaa atactgtcgt
     2581 actctgttcc gagtttgtcc atcagggtcg tctcgatttc ttcgttcaac agcgccagtt
     2641 gtgtagcgat atcctctgcc gtaaagcaga tcttttcacc gaacagttct ggcagacgct
     2701 tgcgagcatc aaagaaaggt ccgacactgg ttccacggtt ttcaggatcg atcaccgtga
     2761 tttccaactt tgccacaggg ctgctagcca gaacaccaaa catccatgca tgcgacagtt
     2821 gctttgcctt ttccgggaaa cgagcgtccg acatcacaag gagtggttca gctcctttac
     2881 atgccatcgg aagcggaaag cggatcacat cgttaaccat catatgggca cagcgctttt
     2941 tcagaaggga caaaagcttt ttattctcga ccagaagcaa aagaggataa tccgtaaagc
     3001 tcatccagag gattccatct gcaaagtttt tcccgctgtt cactttcact ctgcgcgatt
     3061 cataatccat tcccgtctgc gccagaaaat caacaaattc ctgcgggaac agttcatcaa
     3121 gctctctctg cagctgcgtt tcatcgttct tgttcttgct gctc
//
LOCUS       DAOMUF010000211         3154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8740_length_3154_cov_2.835431, whole genome shotgun sequence.
ACCESSION   DAOMUF010000211 DAOMUF010000000
VERSION     DAOMUF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8740_length_3154_cov_2.835431"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /locus_tag="PLN77_09590"
     CDS             complement(<1..1792)
                     /locus_tag="PLN77_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44556.1"
                     /translation="MPYVRSRYYREEEKPAGYRRVEAGFGLLEKNTLFHQLDGYITAK
                     PSHLNAKGSLACVTKDGDIYVNMRANLSPEQWCYVMAHNLLHLAFGHFDKASIPSDCE
                     FVPALWNKACDIYITRFLYDIRLGEPICADPAEAYPIKLNSEQKIYEYLLKHQDNGAQ
                     ICGTNSEKLKDMIGVERPIVYKKGERNAYAEKFSYAVTHSIKSALCDAGGYDLKTKKN
                     TVISRAAEWFLSHYPLLGGLAAAFKIVEDIEMCHRYEIHIAAVDAEQGIIYANPSCGF
                     SEEEWIFVLAHEYLHAGLQHQKRCNGRDFFLWNIACDYVINDWLHEMEIGRMPPDGLL
                     YDETLHNKSAESIYDLIVKEIRKYKKLNTFRGFGRGDMFNGNRPHFTGRENGVSLDEL
                     LKNALREGLDYHQEHNRGFLPAGLVQEIRALAMPVIPWDAALAKWFDVQFPPLEKHRT
                     YARPSRRQGATPDIPRPGYAYYEQDLESRTFGVVVDTSGSMSAKQIGMALGSIASYAA
                     AKDVPFVRIIFCDAKATDAGYLAPEDIAGRVKVTGRGGTILQPGVDCLEQANDFPKDG
                     PILIITDGEIENDLKVRREHAFLLPRGNRLP"
     gene            complement(1903..2958)
                     /locus_tag="PLN77_09595"
     CDS             complement(1903..2958)
                     /locus_tag="PLN77_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRM44557.1"
                     /translation="MHLPISVTQDELLEVLLQVAPVRPVFIWGAPGIGKSALVEKFAD
                     EVGLPCVSLLGSQLAPEDIIGIPQIRGETSEFLPPKMIARKEPYVLFLDELNACTQEV
                     QKAFYSLIHERRIGEYHLPEGSIVIGAGNRAEDSAIVKTMSSALLNRMFHVQLKADVG
                     QWIKWAQAEGLHPWVIDYIIQRPDHLFSEPPKTEEPFSTPRSWHMLSDALKEYRAGEQ
                     DISQETLKMMAYACLLEQHAGMFLAYTKTLRNTHLLDDIIAQKAKWPDKPENRDVLYF
                     LAQSFRARLLAELPKSKQGISGGMLSFAYRAKGMIKELAVINFEIAQMTVAADGAEAL
                     PDWFMMEIIRDLPRLVG"
BASE COUNT          674 a          881 c          733 g          866 t
ORIGIN      
        1 acggcagccg gtttcctctc ggaagcagaa atgcatgctc tcgtcttacc ttcaggtcat
       61 tctcaatctc cccgtccgta ataatcagaa tcggcccgtc ctttggaaaa tcgttcgcct
      121 gttccagaca atccacgccc ggctgcagta tggttcctcc tcgtcccgtg acttttaccc
      181 ggccggcaat atcttccggt gccagatatc ccgcatccgt cgctttggca tcacagaaaa
      241 tgattctcac gaacggaaca tctttcgccg cggcatagct ggcgatggag ccgagtgcca
      301 taccgatctg ttttgcactc atggaaccgg acgtgtccac gaccacaccg aaggtccggc
      361 tctccagatc ctgctcgtaa tacgcatatc ctggccttgg aatatccggc gtggcaccct
      421 gccgtctgct cggccgcgca taggtgcggt gtttttccag cggtggaaac tgtacatcaa
      481 accacttcgc aagcgcggca tcccagggaa tgaccggcat cgcaagcgcc ctgatttcct
      541 gcaccagccc ggccggcagg aaaccacgat tgtgctcctg atggtaatcc aatccctccc
      601 gcagtgcatt tttcagcagt tcatccaggc tgaccccgtt ttctcttcca gtaaaatgcg
      661 gtctgtttcc gttaaacata tcacctctgc caaagccccg gaaggtgttt agctttttgt
      721 actttcggat ctctttcacg atcagatcat agatactttc tgccgatttg ttgtgaagtg
      781 tttcatcata caacagccca tccggcggca ttctgccgat ctccatttcg tgcagccagt
      841 cgttgatgac gtagtcgcag gcgatgttcc acagaaagaa gtcacgccca ttgcaccgtt
      901 tctggtgctg cagtccggca tggagatatt catgcgcgag aacaaaaatc cactcttcct
      961 ccgaaaatcc gcacgacgga ttggcataaa tgattccctg ctctgcgtca accgctgcaa
     1021 tatggatttc ataccgatgg cacatttcaa tgtcctccac aattttaaat gcagccgcaa
     1081 gacctccgag cagcggataa tgggacagga accattctgc tgctctggaa atgacggtat
     1141 tctttttcgt tttaagatcg tatcctcccg catcgcagag tgcagatttg atggaatgag
     1201 tgactgcata ggaaaatttt tcagcatatg catttctttc tcccttttta taaacgatcg
     1261 gccgttctac cccgatcata tcctttaatt tttcagagtt tgtcccgcag atctgcgcac
     1321 cattatcctg gtgcttcaac agatattcat aaattttctg ctcgctgttc agtttgatcg
     1381 gatatgcttc cgccggatct gcgcagatcg gctcgccgag gcggatgtca taaaggaatc
     1441 tggtgatgta gatatcacag gctttgttcc agagtgccgg gacaaattcg cagtcggaag
     1501 ggatgctcgc tttgtcaaag tggccgaacg ccaggtgcag aaggttgtgt gccatcacat
     1561 agcaccattg ttccggcgac aggttcgccc gcatgttgac gtagatatct ccatctttcg
     1621 taacacaggc cagacttcct tttgcattca gatgggaggg ctttgcagtg atgtacccat
     1681 ccagttgatg gaacagtgta tttttttcca gcaaaccaaa gccggcctcc actctgcggt
     1741 acccggccgg cttttcttct tcgcggtaat acctgcttcg aacgtatggc atctgtttct
     1801 ccttcctgtt tttcagatcc gtgtcaattt attttagtgc ttccatttga agctcatgaa
     1861 tcactccccc agtcatgtgt ttcaataata accgggcttt tttcagccga cgagccgcgg
     1921 cagatcacgg atgatttcca tcataaacca gtccggcagt gcctccgctc catcagctgc
     1981 gacggtcatc tgggcgattt cgaaattgat gactgcgagt tctttgatca tgcccttggc
     2041 gcggtacgca aaggaaagca tgccgccgga aattccctgt ttgcttttcg gcagttctgc
     2101 aagcagtctg gcgcggaagg actgtgcgag aaaatacagc acatcccggt tttccggctt
     2161 gtccggccat tttgctttct gcgcgataat gtcatccagg agatgtgtgt tgcgcagtgt
     2221 ttttgtgtaa gcaagaaaca tccccgcatg ctgttccaag aggcaggcgt acgccatcat
     2281 ttttaatgtt tcctgcgaaa tatcctgctc cccggcccgg tattccttca acgcatcgct
     2341 cagcatatgc caggaacgcg gggtggaaaa cggttcttcg gttttcggcg gttcggaaaa
     2401 gaggtgatcc ggccgctgga tgatatagtc aatgacccac ggatggagtc cttctgcctg
     2461 cgcccacttg atccactgtc ccacatccgc tttcagctgc acatggaaca tacggtttaa
     2521 aagggcggac gacatcgttt ttacgatcgc gctgtcctcg gcacggtttc ctgctccgat
     2581 cacaatgctt ccctccggaa gatggtattc tccgatccgc cgctcatgga tcagggaata
     2641 gaaggctttc tgcacttcct gcgtacaggc gttgagttcg tccaggaaca ggacatatgg
     2701 ctcttttctg gcgatcattt tcggcggaag gaattccgag gtctcgcccc ggatctgcgg
     2761 aatgccgata atgtcctccg gcgcgagctg gcttccaagg agcgacacac acggcaggcc
     2821 gacttcgtct gcgaattttt ccactaacgc ggattttccg atgccgggcg caccccagat
     2881 aaaaaccggc cgcaccggcg caacctgaag caatacttct aataattcat cctgtgttac
     2941 actgattggt aaatgcatct gatgtactcc cttctgtttc gatcctgtgt cttttcaaag
     3001 cggatattcg caatccactt taccacttgc ttaatttgac taaatcactt tttctgccct
     3061 gccgtgatct tactgtgctg cattacaacg tgtctcacca tatttctctg tattttccag
     3121 tttcactgtc atattccagc accggaattt ttcc
//
LOCUS       DAOMUF010000212         3147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8771_length_3147_cov_4.958926, whole genome shotgun sequence.
ACCESSION   DAOMUF010000212 DAOMUF010000000
VERSION     DAOMUF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8771_length_3147_cov_4.958926"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(431..2020)
                     /locus_tag="PLN77_09600"
     CDS             complement(431..2020)
                     /locus_tag="PLN77_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM44558.1"
                     /translation="MKKRRSVLALLLVAALTVVLGGCQDGKDGKADSSDTTSSQVSTE
                     STPTGEPVMGGSITVGIPQDIEDSLDPHKSVAAGTKEILFNIYEGLVKPDEEGNLNDA
                     VAESHSISEDGKVYTFKLRNGVKFHDGTTVTAEDVKYSIERCAGINGDGTPLVEAFSN
                     VDKVEIPDESTIDIYLKEADTEFLAYLTVAIVPEHVEDLEADPVGTGPFHYVSRSPQE
                     NIVLEKFSDYWDTENQAYLDKVTFRIVKDSNAVVTNLKSGTLDMYARLSSTQTAQLAE
                     DSDFTIYDGGMNLVQALYLNNAVEPLNNVKVRQALCYAANRQEVLDMIADGKGTIIGS
                     SMFPAFGKYYVPELSERYNQDIEKAKELLKEAGYPDGFELTITVPNNYQQHIDTAQVL
                     VEQLKAIGVTAKIQQVEWDSWLSDVYADRKFQSTVVGVDAAYLTGRALLERFTSTSSK
                     NFINYSNEEYNKLYQQVKTSTDEEEQVELYKKMETLLCDDAANLYIEDMACEVALRSD
                     FAGYRFYPLYVQDMAKIYKVK"
     gene            complement(2259..2726)
                     /locus_tag="PLN77_09605"
     CDS             complement(2259..2726)
                     /locus_tag="PLN77_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRM44559.1"
                     /translation="MLMPSIFGENLFDDFFNDFPFYDYDKEMKNTEKKLYGRKASHVM
                     KTDIRELNNGYEVVVDLPGFTKDEVQATLENGYLTISAEKGLDKDEKEKENGRYIRKE
                     RYAGACSRSFYVGEDITEEDIKAEFKHGLLKLFVPKKEAKPAVEQKKTISIEG"
BASE COUNT          726 a          801 c          599 g         1021 t
ORIGIN      
        1 attgcagcag attcccacca tactggataa aataccatag ctcgtgcgca catccgcatt
       61 gtcggtgttc tcatactctt ttacaaaaca tcttaccaga aaatctgtca tgggttcccc
      121 ttctttccac cctatattaa gaaaaaggcc tcacgcatcg ccgcggagcc tttttgtttt
      181 ccctgtcctt tctatagtag gcagtgttga aaaaaaagtc aagcctaaca tattttcgga
      241 gattctatcg acagagagaa aaagcaacgc agactgcttc ttttcagcaa ttctgcgccg
      301 ctttttctct gttttttcag attttatctc ttttcaggca gatttgacaa tcgaatgaga
      361 gtttttcttt ttcctgcctg aaatagcgtt accgcccacc ggtacccagc gagccgtaac
      421 ggaatagcta ctattttact ttataaattt tcgccatatc ctgcacatac agcggataga
      481 accgatagcc cgcaaaatcg ctccgaagag caacctcgca tgccatatcc tcgatataca
      541 ggtttgccgc atcgtcacag agcagtgttt ccattttttt gtaaagctca acctgctcct
      601 cttcatccgt gctggtctta acctgctgat acagtttatt atactcttcg ttgctgtagt
      661 tgataaagtt cttagatgat gttgaggtaa accgttccag caacgcgcgt ccggtcagat
      721 atgccgcgtc aacgccgaca accgtggact ggaacttccg gtctgcatat acatccgaca
      781 gccagctgtc ccactcgacc tgctgaattt tggcagtaac accgatcgct ttaagctgct
      841 ctaccagaac ctgggctgta tcgatatgct gctgataatt gttcggcact gtaatcgtaa
      901 gctcaaatcc atctgggtat ccggcttctt ttaacagttc ttttgccttc tcaatatcct
      961 gattatatcg ctctgacagt tccggcacat aatattttcc aaatgccggg aacatgctgc
     1021 ttccgatgat cgttcctttt ccgtccgcaa tcatatccag cacttcctga cggtttgccg
     1081 catagcagag cgcctggcgc accttcacat tgttgagcgg ttccacggca ttgttcagat
     1141 acagcgcctg taccaggttc ataccgccgt cataaatcgt aaaatcggaa tcttcggcaa
     1201 gctgcgctgt ctgtgtggaa ctgagacgtg catacatgtc cagtgtaccg gatttcaggt
     1261 ttgttacgac tgcattgctg tccttgacaa ttcgaaatgt taccttgtcc agatacgcct
     1321 gattttccgt atcccagtaa tcgctgaatt tttcaaggac aatattttcc tgcggggacc
     1381 gtgatacata gtggaacggg cctgttccga ccggatctgc ttccagatct tccacatgct
     1441 caggtacaat cgccaccgtc agataggcaa gaaactcggt gtctgcctct tttaaataga
     1501 tgtcgatcgt gctctcgtcc ggaatttcaa ccttgtctac attggaaaat gcctctacca
     1561 gaggcgttcc gtctccgttg atacccgcac agcgttcgat tgaatatttt acatcctctg
     1621 cagtgacggt cgttccatcg tggaacttga caccattccg gagcttgaag gtataaacct
     1681 ttccatcctc cgagatcgaa tggctctctg ccaccgcatc gttcagattt ccttcttcat
     1741 cgggctttac aaggccctca taaatattga ataggatttc tttcgttcct gccgcaacag
     1801 atttgtgtgg gtcaagacta tcctcaatgt cctgaggaat tcctaccgtg atggaacctc
     1861 ccataacagg ttcccctgtc ggggttgatt ccgttgatac ctgagaactt gtggtatcgg
     1921 aagaatctgc tttcccatcc ttgccatcct ggcatccgcc aagtacaaca gtaagagctg
     1981 caactaaaag cagcgcaagt acgcttcttc ttttcttcat gatttctcct tttgatacga
     2041 gttctctcat tttgtaattg tcacgcctat tgtatccgac ctgttcccaa atagcaagta
     2101 cattattgta tacaatttat tttccaaatc cgaaaatcac gtatatccgc cccgcgtatt
     2161 ccggattctt tccttcggga tgtaccggaa aaaataaaaa ttttgccgcc cgggaatttt
     2221 aacattcccg ggcggcaaac gcttttgctt tttttctttt atccttcaat ggagattgtc
     2281 tttttctgct ccacagccgg ttttgcttcc ttcttcggta cgaagagttt cagcagaccg
     2341 tgtttaaatt cagccttgat atcctcttcg gtaatatcct cacctacata gaagcttcta
     2401 ctgcatgcac ctgcgtaacg ctctttgcgg atgtagcgac cgttctcttt ttctttctcg
     2461 tctttgtcaa gacctttctc tgcactgatg gtcagataac cattttccag tgtggcctga
     2521 acttcatctt ttgtgaagcc cggaaggtca accactacct cgtagccatt attcagctcg
     2581 cgaatatctg ttttcatcac atggctggct ttgcggccat acagtttctt ttctgtattc
     2641 ttcatctctt tgtcataatc atagaacgga aaatcgttga aaaagtcatc aaataaattc
     2701 tctccaaaaa tactaggcat taacataagt catcgctcct tcttattctt ctcatggaac
     2761 ctgatccatg ctttttctcc ggtttttctt ttcaccggat ctttttgttt acctgtttct
     2821 ttggaacgga aaggcttccg gataccggag gttttcttct tttcctctct ttgtatccct
     2881 cttcctttgt tctagctgta atataacact tattattagc actgtcaaga ggtgagtgct
     2941 aattattttg tgaagatttt gtgaagcctt gattttacgg gcttcacagg gatttttcac
     3001 ttgctttcgc actcgatttt cttgatttga ccacattttt acgacaaatt cttggaaaaa
     3061 tatattcgtc tgattttctt accacatttt acgacaacga cttatggttt ataccacagc
     3121 aggtacggtt tctggcaggt tcatttt
//
LOCUS       DAOMUF010000213         3129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8846_length_3129_cov_3.560833, whole genome shotgun sequence.
ACCESSION   DAOMUF010000213 DAOMUF010000000
VERSION     DAOMUF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8846_length_3129_cov_3.560833"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2478)
                     /locus_tag="PLN77_09610"
     CDS             complement(<1..2478)
                     /locus_tag="PLN77_09610"
                     /inference="COORDINATES: protein motif:HMM:NF040159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HRM44560.1"
                     /translation="MRRRLEQLLNGRFEYEVPHLLLSETEVALTLDEGQNFRGELNIG
                     AEDGRRVKGIVTTDHQRIVLAKNQFQGTASTIEYGVDTSGLKAGDEICGNITVSSNLE
                     ERCVRVHVSIAGKTMNISGQEIHSLADFVHLASHDFGAAYRFFVKKEFARLLQKEAPE
                     QMALYQGLSHKPVTFQHLEEFLVALGQKEPVMLSAEQPEKTVLTVDQPRKETLYLYKN
                     TWGYVRMEIEVQGDFLEVEKKVVTSEDFIGSVYGVEYIIHQEKIGNGRHYGRITVRQG
                     KQELRFELEVTNSEKHVTSRKNTERDRQITAIARGYLDLAVHKRDYRTWYQDTWEAIE
                     QFEKAGGDMAWVTLGKAWLYESHEETGKARETLSYVKEHQELLDTAEKKGAYLYLAWK
                     LKMEVGHAELVSRLGTLMRQESASYFLLKLAIDADDSWQQSLTRQLYAMELCYECGCK
                     SPLLYLDAAQLYLRQEGQLRRISPFTVQVLKFAQKEGILSGELLKRAAYLTETMKTFD
                     ENVYQILTAGYERFPSDEVLEAICKLVMKGRPVRKEYFPWYAKAVERNIRITRLYEYY
                     IETMDASFDEVLPQVIRMYFSYSNTLAGQKKALIYANVIKNREKDRHTFQSYRPAMEV
                     FTARQIQKGRMNREYAALYRTFLKKPEDPDSAKAIANVLFTHHVTCENPKITRVIVCH
                     PAMAGETSYPMTERGAYIRLYGKKARILLEDEQRRRYAVTETCRVEPLFTDEELARAC
                     GGFAIENPGLLLHLCGEQEEEMQLTAENLAWYQQAARTSAFSSAYRKTVRQKLLEYYL
                     AHPDERETEAAVAELEDSAYAKVDRIST"
     gene            complement(2580..>3129)
                     /locus_tag="PLN77_09615"
     CDS             complement(2580..>3129)
                     /locus_tag="PLN77_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HRM44561.1"
                     /translation="KLIKYMFPKAHAAAYVMMAYRIAWYKVFHPLAYYAAFFSIRATS
                     FSYELMCMGKERLEYYMAEIRKKGDEASKKEQDTLKDMRIVQEMYARGFDFVPIDLYK
                     AKAHRFQIIGNKLMPALDTIEGLGDKAAEAVVDAAAKGKFLSKDDFRDRTKVSKTIID
                     FMADLGVFGDLPESNQFSLFDY"
BASE COUNT          656 a          894 c          721 g          858 t
ORIGIN      
        1 cgtagaaata cggtccacct ttgcgtaagc gctgtcctcc agctctgcaa cagctgcctc
       61 ggtctcacgc tcatccggat gggcaagata gtattccagc agtttctgac gcactgtttt
      121 ccggtaagca ctggaaaatg cgctcgtgcg cgctgcctgc tgataccagg caagattttc
      181 tgcggtaagc tgcatctcct cttcctgctc cccgcacagg tgcagcagca gacccggatt
      241 ttcaatggca aaaccgccgc aggcacgggc aagctcttca tccgtaaaca atggctctac
      301 gcggcaggtt tctgtcaccg catatcttct gcgctgctca tcttcgagaa gaatccgcgc
      361 ttttttgccg tacagacgga tataagcacc ccgttccgtc atcggatagg acgtttcgcc
      421 cgccatcgcc ggatggcaga cgatcacacg cgtgattttc ggattttcac acgttacatg
      481 atgggtaaac agcacatttg cgatcgcttt tgcgctgtcg ggatcttccg gttttttcag
      541 aaaagtccgg tacagtgcgg cgtattcgcg gttcatgcgg cctttctgga tctgtcttgc
      601 cgtgaaaacc tccatcgccg ggcggtagct ctggaatgta tggcggtctt tttcgcggtt
      661 tttgatcacg ttggcgtaga tcagcgcttt cttctggcct gccagtgtgt tgctgtagga
      721 gaaatacatc cggatgacct gcggaaggac ctcgtcgaac gacgcatcca tcgtttcgat
      781 atagtactcg tacaggcgcg tgatgcggat gttccgctcc acggcttttg cataccacgg
      841 gaaatattcc ttccgcaccg ggcggccctt catcaccagt ttgcagatgg cttcgagcac
      901 ttcatcggat ggaaaacgct catagcccgc agtcagaatc tgatagacat tctcatcgaa
      961 cgttttcatc gtctctgtaa gataagcggc ccgttttaac agttccccgc ttaaaatgcc
     1021 ttctttctgc gcaaatttca gcacctgaac ggtgaacggc gaaatccggc ggagctgtcc
     1081 ctcctggcgg agatagagct gtgccgcatc cagatacaga agcgggctct tgcacccgca
     1141 ctcatagcaa agttccatcg cgtagagctg acgggtcagc gactgctgcc aggaatcgtc
     1201 ggcatcaatg gcaagcttta acagaaaata gcttgcactc tcctgccgca tcagtgttcc
     1261 aaggcgtgat accagctcgg catggcccac ttccattttc agcttccatg ccagatacag
     1321 gtacgctcct tttttctccg cggtgtccag cagttcctga tgctctttta cgtaagaaag
     1381 cgtttctctt gcttttccgg tttcctcatg gctctcgtac agccatgctt ttcccagcgt
     1441 cacccacgcc atgtctccgc cggctttttc gaactgctcg atggcctccc aggtgtcctg
     1501 ataccaggta cggtagtcgc gtttatggac tgccagatcg agatagccgc gcgcgatggc
     1561 agttatctga cggtcgcgct ccgtattttt cctgctggtc acatgctttt cgctgtttgt
     1621 cacttccagt tcaaagcgaa gctcctgttt tccctgacgc accgtaattc tgccgtaatg
     1681 gcggccgttt ccgattttct cctgatggat aatgtattcc acaccgtaga cgcttccgat
     1741 gaaatcttct gaagtgacga ctttcttttc cacttccaga aagtctccct gcacctcgat
     1801 ttccatgcgg acgtagcccc aggtattttt atacagatac agtgtttcct tgcgcggctg
     1861 gtccacggta agcacagttt tctccggctg ttccgccgaa agcatcaccg gctctttctg
     1921 accgagggca accagaaatt cctccagatg ctgaaatgtc accggcttgt gggacagtcc
     1981 ctgataaagt gccatctgct ccggtgcctc tttctgaaga agacgtgcaa actctttttt
     2041 tacaaagaag cggtacgctg cgccgaaatc atggcttgcc agatgcacaa aatctgccag
     2101 ggaatgaatc tcctgtccgg aaatgttcat tgtctttccc gcaatcgaga cgtgtacccg
     2161 cacgcagcgt tcctccagat tgctgctgac ggtgatgttt ccacagattt catcgcctgc
     2221 ttttaatccg ctggtatcca cgccgtactc aatcgtactc gccgttccct ggaactgatt
     2281 tttcgccaga acgattctct gatgatctgt ggtcacaatt ccttttactc ttctcccgtc
     2341 ttccgctccg atattcagct ctccgcggaa attctgcccc tcgtccagcg tcagggccac
     2401 ctccgtctcg gacagcagca gatggggaac ctcatattca aatctgccgt tcagcagttg
     2461 ttccaatctt cttctcaatg tttttcacct tattttatct aaatcgtttt cgttcttttc
     2521 cacaacgtct gattataccc caagacgcac gggattgcaa cggaaagggg acgctccttt
     2581 taatagtcga acagcgaaaa ctggttgctt tcgggcagat ctccgaagac acctaggtcc
     2641 gccataaagt cgataatcgt cttgctgacc ttggtgcggt cgcggaaatc atccttggaa
     2701 agaaattttc ccttcgcggc tgcatctacc acagcctctg ccgccttatc tccaagaccc
     2761 tctatggtat ccagcgcagg catcagttta tttccaatga tctggaaccg gtgggccttt
     2821 gctttataaa ggtcaatggg cacaaaatca aaacctctgg cgtacatctc ctgtacgatt
     2881 ctcatatcct tcagtgtatc ctgctccttc ttggatgcct catcgccttt tttgcggatc
     2941 tctgccatat aatattccag gcgttctttt cccatacaca tcagttcata ggaaaaagat
     3001 gtggcacgga tactgaaaaa tgccgcataa tatgccagcg gatgaaacac tttataccag
     3061 gcgatacggt aggccatcat aacgtaggcc gccgcatggg ccttcgggaa catatacttg
     3121 atcagctta
//
LOCUS       DAOMUF010000214         3117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8905_length_3117_cov_2.458850, whole genome shotgun sequence.
ACCESSION   DAOMUF010000214 DAOMUF010000000
VERSION     DAOMUF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8905_length_3117_cov_2.458850"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    263..272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            425..1906
                     /gene="malQ"
                     /locus_tag="PLN77_09620"
     CDS             425..1906
                     /gene="malQ"
                     /locus_tag="PLN77_09620"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRM44562.1"
                     /translation="MRASGILLPVASLPSRYGIGCFSKEAYEFVDRLEEAGQSYWQIL
                     PLGPTGYGDSPYQSFSTFAGNPYFIDLETLVKEGLLTEEECDACDFGDNAEYIDYEKI
                     YLSRFKVLRKAFERFAADDVYDAFVSENGYWLEDYALYMAIKDALGGISWSEWPAELK
                     DREEAALNQKREELAGEVAFYKFQQFMFLKQWKALKAYANEKGIRIIGDIPIYVAFDS
                     ADTWANPVLFQFDEDNQPKAVAGCPPDAFSATGQLWGNPLYKWDYHKSTGYAWWLLRL
                     AHVFKLYDTVRIDHFRGFDEYYSIPFGDQTAERGHWEKGPGMDLFNTVKEKLGDVDVI
                     AEDLGYLTESVIEMVKESGYPGMKVLQFAFDSREESDYLPHNYERNCVVYTGTHDNDT
                     ILGWYYVMSEEDREFSKEYMGNAKSTDEELPWDFIRMSMESVANLAVTPMQEFLGLGT
                     EARINYPSTLGNNWKWRLLPGQFTPEMAKRIHRLTQITARIAK"
     gene            2005..3039
                     /locus_tag="PLN77_09625"
     CDS             2005..3039
                     /locus_tag="PLN77_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM44563.1"
                     /translation="MEQVTIRDIARKCKVGVSTVSRAMNNHPDINPETKEMILKVIAE
                     SNYVPNNSARNLKRSNAKAIAILVKGMDNMFFNNMISVIEEVVRKKKYACIIERVEEK
                     ANEVDSAIEIVKEKRLRGIIFLGGNFTHPHEKMAQIPVPYVISTIGAVSDGGKPCASV
                     SVDDYRESYKMVDYLCGLGHRRIAIITACEDDISIGRLRLNAYRQALLDHGIPYDPDL
                     VFHMTKDDTYSFATGYKVTKEILEKKTEFSALYATADTLAIGACRALKEAGLTVPDDI
                     SVAGFDGIDAGEYYIPSITTIRQPVEQLAAETAKMLFSLISGKEEPCSKVYDGELVVR
                     ESTKSRIAQQ"
BASE COUNT          903 a          629 c          845 g          730 t
ORIGIN      
        1 gggaggcttg aaaaattcat gatttttaag tgcccaaagc ggttccgccc ccccgcatgc
       61 tgtcttttta tgtatggatt tgcatcgtta caaaaatgca aattcgttgt tttgaataaa
      121 cagagtattc aatatttggt aaatatgata agggggttgg gggttgtttt ttgatgcaga
      181 gtatggtatt gtaaatataa cgagttccgg aaacgttacc gacaccgttt cctatcaatg
      241 agcgccattg gaagcaggaa ggnnnnnnnn nnagacgaga taccgacagc cctgttttta
      301 cgaaacaaaa tgagctggct ataggggttg cataagttac aatttgctgc gtatccgtga
      361 acaggaatta tgtatttgtg gggtcggatg gatagaaaaa acgacaagga gaaagggttt
      421 aaaaatgaga gcaagtggta ttttacttcc ggtagcttcg ctgccgtccc gatatggaat
      481 cggatgtttt tcgaaagaag cgtatgaatt tgtggatcgc ctggaggagg ccggccagag
      541 ctactggcag attcttccac ttggaccgac gggatatgga gattcgccgt accagtcgtt
      601 ctctactttt gcaggaaatc catattttat tgatctggag accctggtaa aagagggact
      661 tctgacagaa gaagagtgtg atgcgtgcga tttcggcgac aatgcagagt atattgatta
      721 cgaaaagatt tatctgtccc gtttcaaagt actcagaaaa gcatttgaaa gatttgcggc
      781 agatgacgta tatgatgcat ttgtatcgga aaatggttac tggctggagg attatgcgct
      841 gtatatggcg attaaggatg cactcggcgg aatcagctgg agtgagtggc cggcagaatt
      901 aaaggacaga gaagaagcag cactgaatca gaaacgggaa gaacttgccg gggaggttgc
      961 attttacaaa ttccagcagt ttatgttcct gaaacagtgg aaagcactga aagcatacgc
     1021 aaatgaaaag ggtatccgta tcatcggtga tattccgatt tatgtggcat ttgacagcgc
     1081 tgacacctgg gcaaatccgg ttctgttcca gtttgatgag gacaaccagc caaaagcagt
     1141 tgccggatgc ccgccggatg cgttctccgc aaccggacag ctctggggca acccgttata
     1201 caaatgggac tatcataaga gcacaggtta tgcatggtgg ctgttacgtc tggctcacgt
     1261 gtttaaactg tacgatactg tccgcatcga ccatttccgc ggatttgatg aatattattc
     1321 catcccgttc ggtgatcaga cggcagagcg cggccactgg gaaaaaggac cgggcatgga
     1381 tctgttcaac accgtcaaag aaaagctcgg cgatgtggat gtcatcgcag aagacctggg
     1441 atatcttacc gagagcgtga tcgaaatggt gaaagaatcc ggctatccgg gtatgaaggt
     1501 gctgcagttt gcttttgact cccgtgagga gagtgattat ctgccgcaca attacgagag
     1561 aaactgtgtt gtctacaccg gaacacacga caatgatacc attcttggat ggtattatgt
     1621 catgagtgag gaagaccgcg aattctccaa agagtatatg ggaaatgcaa agagtaccga
     1681 cgaggaactc ccgtgggatt tcattcgcat gtcaatggag agcgtggcaa atctggctgt
     1741 cactccgatg caggaatttt taggtcttgg aacggaggcg agaattaact atccgtctac
     1801 acttggaaat aactggaaat ggcgcctgct tccgggacag tttacaccgg aaatggcaaa
     1861 gagaattcat cggctgacac agattacagc acgtattgca aaataaaaat acgcgccgga
     1921 gagaaagcaa aacaggccgg gaaaacaagc cggaagagat tccggcagaa caggtacaga
     1981 gtttaaagga gaggcctgca gaatatggaa caggtaacaa tcagggacat tgcccgcaaa
     2041 tgtaaggtag gcgtgagtac cgtatcgaga gcgatgaaca atcatccgga catcaatccg
     2101 gaaacaaaag agatgatttt aaaggtcatt gccgaatcga attacgtacc gaacaacagt
     2161 gccagaaacc tgaagcggtc gaatgccaaa gcgattgcca ttctggtaaa aggcatggac
     2221 aacatgttct tcaacaacat gatcagtgtg attgaagagg ttgtgcgaaa aaagaaatac
     2281 gcctgcatta ttgagcgagt agaggaaaag gcaaatgagg tagactccgc gatcgagatc
     2341 gtcaaagaaa aacggctgcg cggcatcatt ttcctcggcg gaaactttac gcatccgcat
     2401 gagaaaatgg cgcagatccc ggttccgtac gtgatcagta caatcggggc ggtttcagac
     2461 ggaggaaaac cgtgcgcgag cgtatcggtc gatgactaca gggaaagcta taagatggta
     2521 gattatctct gtggtctcgg gcaccggcgc attgccatca ttaccgcctg cgaggacgac
     2581 atcagtatcg gacggctccg tctgaatgcg taccgtcagg ctttactgga tcatgggata
     2641 ccttatgacc ctgatctggt atttcatatg acgaaggacg acacgtattc tttcgccaca
     2701 gggtacaagg taacaaaaga aattctggaa aaaaagacgg agttttccgc gctgtatgcc
     2761 accgcggata cgctggcaat cggtgcgtgc cgtgccttaa aagaggcagg gcttaccgtg
     2821 ccggatgaca tttccgtcgc cggatttgac ggaattgatg caggagagta ttatataccc
     2881 agcatcacaa cgatccgtca gccggtggag cagcttgctg cagagacggc gaagatgctg
     2941 ttttccttaa tttcaggaaa agaagaaccc tgcagcaaag tatacgatgg ggaactcgtc
     3001 gtaagggaat ctacgaagtc gcggatcgcg cagcagtaaa atatttgctg tttcgctgcc
     3061 cccaaaagtg attcagaagt gaacagtccc attcagggaa aaactttgca tggaact
//
LOCUS       DAOMUF010000215         3110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8932_length_3110_cov_2.204583, whole genome shotgun sequence.
ACCESSION   DAOMUF010000215 DAOMUF010000000
VERSION     DAOMUF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8932_length_3110_cov_2.204583"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLN77_09630"
     CDS             <1..573
                     /locus_tag="PLN77_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRM44564.1"
                     /translation="LMNFGWVDGSFGMLEAEQLNDSILASIGSVIAPLFAPLGWGDWK
                     MAVAAVSGLIAKENVVGTFGILFGFGEVAEDGAEIWGQLAGSLSTVAAYSFLVFNLLC
                     APCFAAMGAIKREMNNTKWFFTAIGYQTLLAYVVSLCIYQIGNLFIGGGFGIGTVVAV
                     LLIIGFVYLLVRPYKESATLSVSTNKMFSK"
     gene            671..805
                     /locus_tag="PLN77_09635"
     CDS             671..805
                     /locus_tag="PLN77_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HRM44565.1"
                     /translation="MGTFITLVVVVGLVGLAVRSMIKDKKSGKGSCGGNCGQCKGHCD
                     "
     gene            839..1294
                     /locus_tag="PLN77_09640"
     CDS             839..1294
                     /locus_tag="PLN77_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRM44566.1"
                     /translation="MPQEQTKHEARNYHRTQMKRELIIQKLREQGCRITRQRRILLDI
                     ILEEECSCCKEIYYKALQKDSSIGPATVYRMVNTLESIGAISRKNFYKIACGEECFKD
                     QGCTVELSDHTICRLSGEMWMDVMKSGLKAKGYIQNQDIENVIVSAVEG"
     gene            1471..1782
                     /locus_tag="PLN77_09645"
     CDS             1471..1782
                     /locus_tag="PLN77_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HRM44567.1"
                     /translation="MTIKDLKHPGLFAAGVLFGTAGIKALSSKDAKKLYTNCTAAVLR
                     AKKVVMDTVTTIQENAEDIYADAKQINEEREAAEEAVEDVAEETAEEAVEAEAEDTVE
                     A"
     gene            1880..>3110
                     /locus_tag="PLN77_09650"
     CDS             1880..>3110
                     /locus_tag="PLN77_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM44568.1"
                     /translation="MKFYIKHESRGRIRIHLAQKRMSSVQAETLLYYLQNQNQVSFAK
                     VYDRTGDAVICYEGERAAVIRMIQLFHYEEVELPTGLLESSGRALNNEYQEKLISKVI
                     YHFGRKWLLPAPIRAIYTTVVSVKYIWKGIQTLAQGKIEVPVLDATAIGVSMLRGDYG
                     TAGSVMFLLGVGELLEEWTHKKSVGDLARSMSLNVGKVWLKKDGQEILVPSEKIVAGD
                     EIVVHMGNLIPFDGEVSNGEGMVNQASLTGESVPVRRTLGSVVYAGTVLEEGELTILV
                     KQTGGSSRYEKITAMIEESEKLKSGLESKAEHLADRLVPYSLGGTALTYLLTRNATKA
                     LSILMVDFSCALKLAMPISVLSAIREANQHKITVKGGKFLEAVAEADTIVFDKTGTLT
                     KAQPTVAEVVSFSETKSP"
BASE COUNT          901 a          647 c          838 g          724 t
ORIGIN      
        1 ctgatgaact tcggatgggt tgacggatcc ttcggaatgc tggaagccga gcagttaaat
       61 gacagtatcc tggcttccat cggaagcgtg atcgctcctc tgtttgcacc tcttggatgg
      121 ggcgactgga agatggctgt tgcagctgtt tccggactga tcgcaaaaga gaacgttgtt
      181 ggtacctttg gtatcctgtt tggctttggt gaagttgcag aagacggtgc cgagatctgg
      241 ggacagctcg caggaagctt atctacggta gcagcttatt ctttcctggt attcaacctt
      301 ctgtgcgcac cgtgcttcgc agcaatgggt gcaatcaaac gtgagatgaa caataccaaa
      361 tggtttttta ctgcaatcgg atatcagaca ctgcttgcct atgttgtatc cctgtgcatt
      421 tatcagatcg gtaacctgtt catcggcggc ggttttggaa tcggaacggt agttgctgtt
      481 ctgctcatca ttggatttgt atatctgctg gtaagacctt acaaagagag cgcaactctg
      541 agcgtttcca ccaacaaaat gttcagcaaa taattgctgt gacagataga gaacatgtat
      601 ctgatgaaga gtccgtatcc ggcatccgct gcaggatgcg gactcttttg aaaagaaagg
      661 agttgtttct atgggaactt ttatcacact tgtagtcgtt gttggtctgg taggacttgc
      721 agtacgcagt atgattaaag ataaaaaaag cggcaaagga agctgtggtg gtaactgcgg
      781 gcagtgcaag ggacattgcg actgacagga caggaatcat catcaggagg tagaacgtat
      841 gccgcaggaa cagacaaagc atgaagccag aaattatcac agaacacaga tgaagcgaga
      901 actgattatt cagaagctcc gggaacaggg atgcagaata accagacaga gacggattct
      961 gctcgatatt atcctggaag aggagtgctc ctgctgtaaa gaaatttact ataaagctct
     1021 gcagaaagac agcagcatcg ggcctgctac ggtgtatcgc atggtgaata cactggagag
     1081 catcggtgca atcagccgca aaaattttta caagattgcc tgtggagaag aatgcttcaa
     1141 agatcagggc tgtaccgtag aactgagcga tcataccatc tgccgcttaa gcggagagat
     1201 gtggatggac gtgatgaaat ccggactgaa ggcaaagggc tatattcaga atcaggatat
     1261 tgaaaatgtg attgtttcag cggtggaagg ctgaaaaacg gggaaaacgg gtgaaaattc
     1321 cttccgtttt ccctgtttta atgaaaataa ttatcaaaaa gaactgtacg tattgtctaa
     1381 ccgagcaggt gagtgctata ctgtgaacca cagcggggga aaatgtaaaa ttcaggaaaa
     1441 atttatttta ataacaggag gaaatgtaat atgacaatca aagatttaaa acatccagga
     1501 cttttcgcag caggagtatt attcggaaca gcaggtatca aagcactgtc cagcaaggat
     1561 gcgaaaaaac tttacaccaa ctgcaccgca gctgttcttc gtgcaaagaa agttgttatg
     1621 gatacggtaa cgaccatcca ggagaatgcg gaggacatct atgctgacgc aaaacagatc
     1681 aacgaagagc gtgaagcagc agaagaggca gtagaggatg tagctgaaga gacagcagag
     1741 gaagctgttg aagcagaagc agaggatacg gttgaggcat aagctgtaca cagcaacatc
     1801 tgatttacgt cgtattcata cggaaaaaac aaaacgcctg cagaattctc tgcaggtgtt
     1861 tttctaggag gcataagatt tgaaatttta tattaaacac gaaagcagag gcagaatccg
     1921 aatccatctg gcacagaagc gcatgtcctc cgtacaggca gagacactgc tgtactatct
     1981 gcagaatcag aaccaggtat ctttcgcgaa ggtatatgac agaaccggcg atgccgtgat
     2041 ctgttacgag ggagaacgag ccgctgtgat tcggatgata cagctcttcc actacgagga
     2101 agtagagctg ccgaccggac tgcttgagag ctccggcaga gcgttaaaca acgaatatca
     2161 ggaaaagctg atcagcaaag tgatttacca ctttggaaga aaatggcttc ttccggcacc
     2221 gatccgtgcc atctatacta cggttgtttc cgtaaaatat atctggaaag gaatccagac
     2281 actggcacag ggaaagatcg aagttccggt tcttgatgca accgcaatcg gtgtttcgat
     2341 gctccgcggc gactatggaa ccgcaggctc tgtcatgttc ctgctcggcg taggagagct
     2401 gcttgaggaa tggacgcata aaaaatccgt cggagatctg gcacgcagca tgtcgctgaa
     2461 cgtcggaaag gtatggctga aaaaagacgg ccaggaaatt ctcgttccgt ctgaaaaaat
     2521 tgttgcgggc gacgagattg tcgtacatat gggaaatctg attccattcg acggcgaggt
     2581 atccaacggc gagggtatgg taaaccaggc ttctctgacc ggagaatctg ttccggtacg
     2641 ccgtacctta ggttccgttg tctatgcggg aacggttctt gaggagggcg agcttaccat
     2701 tcttgtgaag cagaccggcg gatccagccg ttacgagaag attaccgcga tgatcgaaga
     2761 gtccgagaaa ctgaaatccg gactggagag caaggcagag catctggcag accgtcttgt
     2821 accgtattcc cttggcggaa cggcgttgac gtatcttctg accagaaatg caacgaaagc
     2881 actctctatt ctgatggtag acttctcctg tgcgttaaaa cttgccatgc cgatttccgt
     2941 tctctctgca atccgcgagg caaaccagca caagatcacc gtaaagggcg gtaaattcct
     3001 ggaggctgtg gcggaagcgg atacgatcgt tttcgataag accggaacac tgacaaaagc
     3061 gcagccgacc gtagcagagg tggtatcctt cagcgaaaca aagagcccgg
//
LOCUS       DAOMUF010000216         3103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_8952_length_3103_cov_4.973097, whole genome shotgun sequence.
ACCESSION   DAOMUF010000216 DAOMUF010000000
VERSION     DAOMUF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8952_length_3103_cov_4.973097"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="PLN77_09655"
     CDS             complement(<1..1308)
                     /locus_tag="PLN77_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44569.1"
                     /translation="MTKTVQKSKARDMTQGSIWKQLLLFSLPLMAGNLFQQLYNTVDS
                     IVVGNFVGKEALAAVGSVGPIINSLIGFFMGLSTGAGVIISQYYGAKADEKVSRTVST
                     TLVMTFILSIVFTILGILITPYMLRMMSTPDDVIRESATYLKIYFGGVAGLMFYNMGS
                     GVLRAVGDSRHPLYFLIFSAVVNTVLDLLFVVSFGMGIEGVALATVIAQCLSAVLTLY
                     VLTTTDGPYRICWKKLCMDWSLLYRIVCVGLPAAIQQMVTSVSNVFVQSYINVFGSDV
                     MAGWSACMKIDQFMMLPMMSVGLASTTFTGQNVGASYIDRAKKGATTAFLLAELVTIV
                     MMIPLLIFAPQMVYLFNQEAAVIRYGTLFLYLLSPFYILCCVNQVYSNVLRGAGDTRT
                     PMIVMLGSFVVFRQIYLFVMSHIFDSTIPIALGYPAGWLVCSVL"
     gene            1608..>3103
                     /locus_tag="PLN77_09660"
     CDS             1608..>3103
                     /locus_tag="PLN77_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HRM44570.1"
                     /translation="MGDYFVCSKTDPVVETKAGKVRGFRLNTTYAFHGIHYAEADRFQ
                     MPQPVKPWKGIKNALAYGYVCPLLKQDEPNMEVLVPHRYWPQDEHCQNLNVWTQSLDP
                     GAKKPVMVWLHGGGFSAGSAIEHVAYEGDHLSEFGDVVVVSVNHRLNILGYLDLSPFG
                     EKYKNSANAGNADMVAALQWVHDNIAAFGGDPENVTIFGQSGGGMKVATLMNTPAADG
                     LFQKGIIESGVYEAKVYQKEDGDGTAIVKALLKELNLDESEVEKLETIPYYELSNAYN
                     KVEAEVAAKGCYIGQGPMENGWFHGNPIKCGFTDHAKTIPVLAGTNIGEFDFGPVVPG
                     KHEMNREEQIAFLTRKYGDATPELISLFEKAYPDKTIADLWSVDTFFRPATIEFIRQK
                     SEFTEAPTYSYQFTYEFPIDGGKAAWHCAEIPFVFHNIDRIAVCNVPGETDRLQERMA
                     TAWINFARYGSPETPSLPNWPACTPGDEATMIFDRTCEIRRNFDHELV"
BASE COUNT          868 a          709 c          850 g          676 t
ORIGIN      
        1 cagaacactg cataccagcc atcccgccgg ataaccaagt gcaatcggaa tggtggaatc
       61 aaatatatga gacatcacaa acaggtaaat ctggcggaac acgacgaagc ttccgagcat
      121 tacgatcatt ggtgttctgg tgtctcctgc gccacgcagg acgttggaat aaacctggtt
      181 gacgcagcag agaatgtaga acggggatag cagatacaga aacagggttc cgtaacgaat
      241 cacagctgcc tcctggttaa acagatagac catttgcgga gcgaaaatca gcagaggaat
      301 catcatcaca atggttacaa gctcagcaag caggaatgcg gttgtagcac ccttttttgc
      361 acggtcaatg tagctggcac cgacgttctg tccggtgaag gtggtcgaag caagaccaac
      421 actcatcatc ggcagcatca taaactggtc gattttcatg caggcactcc agcctgccat
      481 aacatccgag ccgaatacat tgatatagga ctgcacgaaa acgttggaaa cggaagtcac
      541 catctgctgg atcgctgccg gcagtccgac gcagacgatg cggtacagca aagaccagtc
      601 catgcagagc tttttccaac agatgcggta tgggccgtct gttgtggtca gcacatacag
      661 cgtcagaacc gccgaaaggc actgtgcaat aacggttgca agcgccacac cctcgattcc
      721 catgccgaag gaaacgacaa acaaaagatc gagcacggta ttgacaacag cggaaaaaat
      781 caggaaatac agcggatggc gggaatcgcc gacagcgcgg agaacgccgg agcccatgtt
      841 gtagaacatc agaccggcaa caccgccaaa gtaaatttta agatacgttg ccgactcacg
      901 gatgacatcg tccggtgtgc tcatcatgcg gagcatgtac ggcgtgatga gaatgccgag
      961 aatggtaaag acaatggaga gaataaacgt catcaccagt gtcgtgctga cggtgcggct
     1021 gaccttttca tccgcctttg cgccataata ctgagagata atgacaccgg caccggtgga
     1081 aagtcccatg aaaaagccga tcagagaatt gatgatcgga ccgacggagc cgacggcggc
     1141 gagtgcttcc ttgccgacaa agttgccgac aacgatgctg tcaacggtgt tgtaaagctg
     1201 ctggaagaga tttccggcca tcagcggaag cgagaagaga agaagctgtt tccagataga
     1261 gccctgggtc atatcgcggg ctttgctctt ttgtactgtt ttcgtcatag tagtcccctt
     1321 ttttacgttg attttacctt cttttttatt gtagcacttt gaagttgtga tggaaatatc
     1381 catttttgac gtgattttcg gactttgttg atataaacag ccgaaaataa aagtaaaaag
     1441 aattgtttct gaaaaaattg tctggtataa tagggagcag atgcgtaaat gcaaaaacaa
     1501 cagatgcttc aaagaccggt gcaaaaacgg aaagatcagg gggattttgc ggtggttttt
     1561 tggaaactgc gcgtcacaga acaggataga aagaaggtaa ctgaaatatg ggagattatt
     1621 ttgtatgcag taaaacagat ccggtagtag agacaaaggc gggaaaggta cgcggcttcc
     1681 gcctgaatac gacatacgcg tttcatggaa tccattacgc tgaggcggac cgtttccaga
     1741 tgccacagcc ggtgaaacca tggaaaggaa tcaaaaatgc gctggcatac ggctatgtat
     1801 gtccgctttt aaagcaggat gagccgaaca tggaggtgct ggtaccgcat cgttactggc
     1861 cgcaggacga gcattgccag aacctgaatg tctggacaca gagtcttgat ccgggtgcga
     1921 aaaagccggt catggtatgg ctccacggcg gcggcttcag cgccggctct gcgattgagc
     1981 atgtggcata tgagggggat cacttaagcg agttcggtga tgtcgttgtt gtttccgtga
     2041 accatcggct gaatatcctc ggttatctcg atctctcgcc gtttggcgaa aaatacaaaa
     2101 attctgcgaa tgcaggaaac gcagatatgg tagctgcatt gcagtgggta catgacaata
     2161 tcgcggcatt cggcggagac ccggagaatg ttacgatttt cggtcagtcc ggcggcggta
     2221 tgaaggtagc aacgctgatg aatacgccgg cagcagacgg cctgttccag aagggtatca
     2281 tcgagagcgg tgtttatgag gcaaaggttt atcagaaaga ggacggagac ggcaccgcga
     2341 ttgtgaaggc tctgttaaaa gaactgaatc tggacgagtc cgaggtggag aagcttgaga
     2401 cgatcccgta ttatgagctg tcaaatgcgt acaataaagt tgaggcagag gttgctgcga
     2461 aaggctgcta tatcggacag ggaccgatgg agaatggctg gttccacgga aatccgatca
     2521 agtgcggatt tacggatcac gctaaaacga ttcctgtgct ggcaggaacc aacatcggtg
     2581 agtttgattt tggaccggtt gtgccgggaa aacatgagat gaaccgtgag gaacagattg
     2641 cgttcctgac gagaaaatat ggcgatgcaa cgccggagct gatttctctt tttgaaaaag
     2701 catatccgga caaaacaatt gccgacctgt ggtctgtgga tacgttcttc cgtccggcaa
     2761 cgatcgaatt cattcgtcag aaatcggagt ttacagaagc gccgacctat tcctatcagt
     2821 ttacctatga attcccgatc gatggcggaa aagccgcatg gcattgtgcc gagattccgt
     2881 tcgtattcca taacatcgac cgcatcgccg tatgcaatgt cccgggcgaa acagaccgtc
     2941 tgcaggagcg tatggccacc gcctggatca actttgcccg ctacggaagc ccggaaaccc
     3001 catccctccc gaactggccg gcatgcaccc caggggatga agcaaccatg attttcgaca
     3061 gaacctgtga aatccgccgc aacttcgatc acgagctggt gaa
//
LOCUS       DAOMUF010000217         3070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_9098_length_3070_cov_2.657048, whole genome shotgun sequence.
ACCESSION   DAOMUF010000217 DAOMUF010000000
VERSION     DAOMUF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9098_length_3070_cov_2.657048"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..209)
                     /locus_tag="PLN77_09665"
     tRNA            complement(125..209)
                     /locus_tag="PLN77_09665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Leu,seq:aag)
     gene            413..1420
                     /locus_tag="PLN77_09670"
     CDS             413..1420
                     /locus_tag="PLN77_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRM44571.1"
                     /translation="MNLRESFEEREFQELSPYAAHSRNSRGRDRYEPECDIRTVYQRD
                     RDRILHSKAFRRMKDKTQVFLAAQGDHYRTRLTHTLEVSQIARTIARALNLNEDLTEA
                     IALGHDLGHTPFGHSGEAALNAVHPGGFRHAEQSVRVVEVLEKEGQGLNLTWEVRDGI
                     LNHRTAGKPSTLEGQVVRLCDKIAYIHHDMDDAIRAGILSEDDIPITIRVVLGDTCRE
                     RLNTLIHDIVEQSRGTEYVRQSAEIAEGMRMLRELMFANVYRNPEAKKEEEKAKKMLI
                     SLYTYYQEHPEQMSREYRNLIGCGEDKTQVVCDYIAGMTDQYSMHKYEELFVPKAWAV
                     Y"
     gene            1437..>2103
                     /locus_tag="PLN77_09675"
     CDS             1437..>2103
                     /locus_tag="PLN77_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRM44572.1"
                     /translation="MRYSDDLVEEIRMKNDIVDVISGYVKLTRRGSSYFGLCPFHNEK
                     SPSFSVSPGKQMYYCFGCGAGGNVFTFLMEYENFTFVEALKFLADRAGVELPQMEYSK
                     EAKEQADLKNTLLEINKQAAKFYYYQLHTERGKQGYDYLKGRGLSDDTIRKFGLGYSD
                     KYGSLLYRFLKEKGYDDEILRQSGLFLADEVHGMQDKFWNRVIYPIMDANSRVIGFGG
                     RVMG"
     assembly_gap    2104..2143
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2144..>3070
                     /locus_tag="PLN77_09680"
     CDS             <2144..>3070
                     /locus_tag="PLN77_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRM44573.1"
                     /translation="KIFDKSRNLYGLNLARSSRRKNLIICEGYMDVISMHQAGFNNAV
                     ASLGTALTSLQAGLMKRYTDEVLVIYDSDEAGVKAALRAIPMLKGVGLTTRVVNLRPY
                     KDPDEFIQHEGCEAFEKRLEEAENSVLYEIRMKERDFDLADPQGKSDFLHEAVRRLVA
                     IEDEIERNSYLEAVARKYGIAVEVLRKQVGQMALSGAGRTGSVKPRNTASNKKEKEGG
                     VEKAQKLMLTWMTSYPALIGKLEKYITPADFTTPLYHQIAEMIVLQYHEGGVNPAKLL
                     NQFVDSEEQKEVAGLFNARIPLETEEEKRRAL"
BASE COUNT          802 a          714 c          871 g          643 t
ORIGIN      
        1 cttgtgtttg caagggtttt ttcctactca aaaagaagag cagcagaaat ttttatctgg
       61 attttatctg gtctgattct ttttgagtag gaaaaaaccc ttgcatacac aagggttctc
      121 attctgcgga agatgggact tgaacccaca cgatcgcaat gaccaccaga tccttagtct
      181 ggcctgtctg ccaattccag cacttccgca tgttctttaa gtttttgtca tctcgctgac
      241 gacaagatgt attatagcac cagagttgta agccgtcaac actttttttg aaaaaatttg
      301 aaaaatttga aaaataaaaa aggaaatcgg aatcgggcac cgaatatagg aaataggagg
      361 acaatgaaaa gaactgttac aggcatatta ccgggaaaga ggtggtcttc gtttgaactt
      421 gagagaatcg tttgaagaac gggaatttca ggagctgagt ccttatgcgg cgcacagccg
      481 gaattccaga ggaagggacc gatatgaacc ggagtgtgac atccgcaccg tctaccagag
      541 agaccgggac cggattctcc actccaaagc ctttcgcagg atgaaagaca aaacacaggt
      601 gtttctcgct gcgcagggcg atcattaccg gacccgcctg acgcacacac tcgaggtgtc
      661 ccagattgcc cgcacgattg cgcgggcgct gaatctgaat gaggatctga ccgaagccat
      721 tgcgcttggc cacgatctcg gacacacgcc gtttggccat tccggggaag cggcgctcaa
      781 tgcggtgcat cccgggggct tccgccacgc ggaacagagc gtccgtgtcg tggaggttct
      841 agaaaaggaa ggacagggcc tgaacctgac ctgggaagtg cgggatggaa ttctgaacca
      901 ccgcacggcg ggaaagccgt cgaccctgga gggacaggtt gtgcgtctct gcgacaagat
      961 cgcctacatc caccacgata tggacgatgc catccgtgcc ggcattttaa gcgaggatga
     1021 catcccgatt acgatccgcg tcgtccttgg cgatacatgc cgcgagcggc tcaatacgct
     1081 gatccacgac attgtggagc agagccgcgg aacggagtat gtgcgccagt cggcggagat
     1141 tgccgagggc atgcgcatgc tgcgggagtt gatgttcgcg aacgtctacc gcaacccgga
     1201 ggcgaaaaaa gaggaggaga aggcaaagaa gatgctgatt tccctctaca cctactacca
     1261 ggagcacccg gaacagatgt cccgcgagta ccggaatctg atcgggtgcg gcgaggacaa
     1321 gacacaggta gtatgcgatt acattgccgg tatgaccgat cagtattcca tgcacaaata
     1381 cgaggagctg ttcgtgccga aagcctgggc ggtttattag atacagggag gaaaaaatgc
     1441 gatattccga tgatttagtg gaagagattc gtatgaaaaa tgacatcgta gatgtgatat
     1501 ccggctacgt aaagctgaca agaagaggaa gctcttactt cgggctctgc cctttccaca
     1561 atgagaagtc cccttccttt tccgtcagcc caggaaagca gatgtattac tgcttcggat
     1621 gcggagccgg aggaaatgtt ttcacatttt tgatggaata cgaaaacttc acgtttgtgg
     1681 aagccttgaa atttctggcc gaccgggccg gggtggagct tccgcagatg gagtattcga
     1741 aagaagcaaa ggagcaggcg gatttaaaga acacgctgct ggaaatcaac aaacaggcgg
     1801 cgaaattcta ttattaccag ctccacaccg aacgcggaaa acagggctat gattacttaa
     1861 aggggcgcgg tctttcggat gatacaatcc ggaagttcgg tcttggctac tcagataagt
     1921 acggaagcct gctgtaccgc tttttaaaag aaaaagggta tgacgatgaa atccttcgcc
     1981 agtcgggatt gtttctggcg gacgaagtgc acggtatgca ggataagttc tggaatcgtg
     2041 tcatctaccc gatcatggat gccaacagcc gcgtgatcgg cttcggaggc cgtgtgatgg
     2101 gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaagatc ttcgacaaaa
     2161 gccgcaacct ctatgggctg aacctggcgc gctcttcgcg gaggaaaaat ctgatcatct
     2221 gcgagggcta tatggatgtc atctccatgc accaggcggg cttcaacaac gcggtggcat
     2281 ctcttggaac ggcgctcacc tcgctgcagg cagggctcat gaagcggtac accgacgagg
     2341 ttcttgtgat ctacgacagc gatgaggcgg gtgtcaaagc agcactccgt gcgatcccga
     2401 tgctgaaagg cgtcggtctt accacgcgtg tcgtcaattt aaggccgtat aaggaccccg
     2461 atgagtttat ccagcacgaa ggctgtgagg cgttcgaaaa acgcctggag gaggcggaaa
     2521 acagcgttct gtacgagatc cgcatgaagg agcgtgactt tgaccttgcc gatccgcagg
     2581 gcaaatccga ttttcttcat gaagcggtgc gccgtctcgt tgcgattgag gatgagatcg
     2641 agcggaacag ctatcttgag gctgtggcga gaaagtacgg cattgccgtc gaggtactgc
     2701 gcaagcaggt cggccagatg gcactctcgg gagccggaag gacggggagc gtaaagccgc
     2761 ggaatacagc ctcaaacaaa aaagaaaaag agggcggcgt cgaaaaagca cagaagctga
     2821 tgctgacgtg gatgacctct tatccggcgc tcattggaaa gctggaaaaa tatattacgc
     2881 cggcggattt taccacccct ctctaccacc agattgcaga gatgattgtg ctgcagtacc
     2941 acgagggcgg cgtaaatccg gcaaagcttt tgaatcagtt tgttgattcc gaggagcaga
     3001 aagaagttgc aggccttttt aatgccagaa tcccgctgga gacagaggag gaaaaacggc
     3061 gcgcgctgca
//
LOCUS       DAOMUF010000218         3035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_9257_length_3035_cov_3.368792, whole genome shotgun sequence.
ACCESSION   DAOMUF010000218 DAOMUF010000000
VERSION     DAOMUF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9257_length_3035_cov_3.368792"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLN77_09685"
     CDS             complement(<1..260)
                     /locus_tag="PLN77_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM44574.1"
                     /translation="MKQEIIADLSRITEEEQAILDGKTEVEKERYSSGQKFVMDAGKL
                     LERGKLIDIRPHTRFIRFPAHSHNYVEMIYMCQGSTVHTING"
     gene            615..1046
                     /locus_tag="PLN77_09690"
     CDS             615..1046
                     /locus_tag="PLN77_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM44575.1"
                     /translation="MEQQTYTIREMSERFGLPASTLRYYEEIGLLTDVIHTESKKRIY
                     TQQHIDCMTAILCFKRTGLPIAGIQEFFRLEDDIPGNIDQMVAILEEHEQNIREKIER
                     MESDLEHIQQKVRYYSAVRDAIKNGDEMPSWDSCGCEEKAQ"
     gene            complement(1166..2974)
                     /locus_tag="PLN77_09695"
     CDS             complement(1166..2974)
                     /locus_tag="PLN77_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HRM44576.1"
                     /translation="MKKEHSSRWRKLDNAAQAFPAATGKKDTRVFRFYCQLKEDIQAD
                     LLQKALEETMEHYPVFSMVLRKGLFWFYLEQRDLPAKVEEEKRPPCSEIYVPDHKTLL
                     FQVSYYKTRINFEVFHALTDGTGAMLFLKELVSNYLILCHPEEIFSKVSEDMLTETDF
                     EEDSFSQYYTGKKSEKEKSRPAYQIKGEYLEQEEMEITEILLSAEAVHKCAKAHGVSV
                     TAYLAAALVYAVYEEIPKSRLKKPVSLMVPANLRNFFPSASMTNFWSWIEIACDLGPE
                     ASFEDALQITGAAMQKEALKQEISTRMNDLVRIERNPVLRAVPLEIKNLALMAGTTLG
                     GRSITTVYSNIGRIQMPPEYETYIERFGFFTSTDKVQMCSCSYGDSMVLGITSKIADS
                     NIERNLMHLLQKEGIVCEQEENDFPGQKEQPHGTAKLGLKIFSFTCIAAVVLCWMMNF
                     LATPQMWWAGYATAGVFCAWLLIRVGYQKRKNPLKNSMWQLIFIMIGAILWDYATGWI
                     GWSVDFAIPLAVLLNGATMQILARAYKMEVSEYLFYLMQSGAAGIVPAILWLTGTVRI
                     TWPSVICVGLSVLYLIGLFFFRGKDFMREMQKKFRV"
BASE COUNT          734 a          824 c          646 g          831 t
ORIGIN      
        1 tccccgttga tggtgtgcac ggtgcttccc tggcacatgt agatcatctc gacataattg
       61 tggctgtgcg ccggaaaacg aataaaccgc gtgtgcggcc gaatatcaat caattttccc
      121 cgctccagca gctttcccgc atccatcaca aacttctgcc cgctcgaata ccgctccttt
      181 tccacttccg tcttgccgtc cagaatcgcc tgttcctcct ccgtaatccg ggacaggtct
      241 gctataattt cctgtttcac gatgattcct cacatttttt cgttttttct gtcttctatt
      301 atattccatt ctgatacaga taagcaatgc actttttatc ccctgccatg aaaaaagact
      361 ccgatggatt ttcactccgt cagagcctta tatccccata gctgaagcaa ggggttttac
      421 gacgcattgg ataagcaatg tactttttct tctcactttc tgataaatat tcctttccta
      481 cgcatcaaaa cagcaagtgc tgcactgcca agtgaaacgt aacgaagatt ctctctttac
      541 aatttcactt tcttaaagta tactttaaga agttattcta ctattcaaac tctatcgttc
      601 aaggaggttt ttccatggaa cagcagacct acaccattcg tgaaatgtcc gagcgttttg
      661 gacttccggc ctctacgctt cgttattatg aggagatcgg gcttttgacg gatgtcattc
      721 ataccgaaag taagaaacga atttacaccc agcagcacat tgactgcatg accgcaattc
      781 tctgcttcaa gcgcaccggt cttccgattg cggggattca ggagtttttc cggcttgagg
      841 atgatatccc gggaaatatt gaccagatgg ttgccattct cgaggaacat gagcagaata
      901 tacgcgaaaa aatcgagcgc atggaatccg atctggagca tattcaacag aaagtgcggt
      961 attacagcgc ggttcgcgat gcgattaaaa atggggatga aatgccgtcg tgggattcct
     1021 gcggctgtga ggagaaggcg cagtagattt ctctccaggc ttttccgccg cagacacaca
     1081 caagtttcaa aagagcaggt aagaaaaggg accaacatcc acttttctta cctgctcttc
     1141 ttattttttc tatcaccttt tcccttcata cccggaattt tttctgcatt tcccgcataa
     1201 aatcttttcc gcggaaaaaa aacagcccga tcagatacag cacactgaga cccacgcaga
     1261 tcacggacgg ccaggtaatg cggacagttc ccgtcagcca caaaatcgcc ggaacgattc
     1321 ctgctgctcc ggactgcatc agataaaaaa gatattcact gacctccatt ttgtacgctc
     1381 ttgccagaat ctgcatcgtc gcgccattca gaagaacggc gagcggaatt gcgaaatcga
     1441 cagaccagcc gatccagccc gtcgcataat cccacagaat cgcaccgatc ataataaaaa
     1501 tcaattgcca catactgttt ttgagtggat ttttccgttt ctgatatccc acccggatca
     1561 gaagccaggc gcagaacaca cccgctgtcg cataacctgc ccaccacatc tgcggcgttg
     1621 caaggaaatt catcatccag cagagtacga cagccgcgat gcaggtgaaa gaaaaaattt
     1681 tcaatcccag tttggccgtc ccgtgcggct gttctttctg tcccggaaaa tcattttcct
     1741 cctgttcaca gacaatgccc tctttctgca gcagatgcat caggttccgt tcaatattgg
     1801 aatcggcgat tttagatgtg attccaagca ccatgctgtc cccgtaggag caggaacaca
     1861 tctgtacttt gtctgtgctg gtgaaaaatc cgaaccgctc aatatatgtt tcatactccg
     1921 gcggcatctg aatccggccg atattggaat agacggtcgt aatgcttcgt ccgccgagcg
     1981 tcgtgcccgc catcagcgcc agatttttga tttccagcgg cacggcgcgc agcaccggat
     2041 tccgctcaat ccgcaccaga tcgttcatac gtgtggaaat ctcctgcttc agcgcctctt
     2101 tctgcatcgc ggctcccgtg atctgcaggg catcctcaaa cgatgcctcc ggcccaagat
     2161 cacaggcaat ctcgatccag ctccagaaat tcgtcatcga tgcagatggg aaaaagttgc
     2221 ggagattggc tggaaccatc agagaaaccg gctttttcag cctgcttttc gggatttctt
     2281 catataccgc atataccagc gccgccgcaa ggtacgccgt taccgacacg ccatgtgctt
     2341 ttgcgcactt atgaactgct tccgcagaaa gcagaatctc agtaatttcc atctcctcct
     2401 gctccagata ctcgcctttg atctggtatg ccgggcggct cttttctttt tcacttttct
     2461 ttccggtgta atactgggaa aagctgtcct cctcgaaatc tgtctctgtc agcatgtctt
     2521 cgctgacctt tgaaaagatt tcctcgggat gacacaggat cagataattg ctcaccagtt
     2581 ctttcagaaa cagcatcgca ccggtgccgt ctgtcagggc atggaatact tcgaaattga
     2641 ttcgggtttt atagtaagaa acctggaaaa gaagcgtttt gtggtcgggt acgtaaatct
     2701 cactgcacgg cggacgcttt tcctcttcta cttttgctgg cagatcgcgc tgctccagat
     2761 agaaccagaa aagacctttc cgcagtacca tggaaaacac cgggtaatgc tccattgtct
     2821 cctccagcgc tttctgcagc agatccgcct ggatgtcctc tttcagttgg cagtaaaacc
     2881 ggaagactct cgtatccttt tttccggtgg ccgccggaaa tgcctgtgcc gcgttgtcca
     2941 gctttctcca gcgcgaagaa tgttctttct tcattttcta tgcctcctta cgccttccgg
     3001 tagtgtcaaa aactacctgt tttttattgt tctaa
//
LOCUS       DAOMUF010000219         3008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_9355_length_3008_cov_2.564511, whole genome shotgun sequence.
ACCESSION   DAOMUF010000219 DAOMUF010000000
VERSION     DAOMUF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9355_length_3008_cov_2.564511"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PLN77_09700"
     CDS             <1..1021
                     /locus_tag="PLN77_09700"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HRM44577.1"
                     /translation="ATEPPAPTEDPAPEPLVPEAIEPTPAEDALPEGMVKSYLTGEPV
                     AEVQGRRRPVAVMLSNIISACPQSGISRAGVIYEAPVEGGITRLMGLFENYDDLEKIG
                     SVRSCREYYVYLAAGFDALYYHYGQAAYAIPLLESDHIDNVNGMEYSEQVYYRTSDRP
                     SPHNAYLDAAGIAVGIDLCGYRTSYKDGYDGFFQFASETEPETLEQGRNATYVAPGGY
                     FHNNPWFSYDEVSGTYLRYQFGEPQIDEMTQEQLAVKNIIVQYCSWKKYDDNAEYLDI
                     DVVNGGTGTYITSGKAIDVTWSKDDPWGVTKYRDGNGDEIKLNPGKTWVLIVLDNYAD
                     ETEIR"
     gene            1128..1346
                     /locus_tag="PLN77_09705"
     CDS             1128..1346
                     /locus_tag="PLN77_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HRM44578.1"
                     /translation="MEINIQRINELARKAKAEGLTPAEKEEQQKLRREYIAAVKMNLR
                     TQLDNIDMQEKDGTIVNLGERYGSKRKH"
     gene            1324..1872
                     /locus_tag="PLN77_09710"
     CDS             1324..1872
                     /locus_tag="PLN77_09710"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRM44579.1"
                     /translation="MEAKGNIRKEVFARRRAATQEEIQEKSRMIYEKITALPEFLDAD
                     CFFAYMDFKKEVMTRDLIEKALQMGKTVAVPRVEGDDMVYYEIKDFSTLKSGYFGIME
                     PDGGNVCMREEGFLLVPGVAFDPARHRVGYGKGFYDRFLAAHPGFTTVAAAFEFQLFD
                     AVPFEETDILPQMLVTEQKIYR"
     gene            1913..>3008
                     /locus_tag="PLN77_09715"
     CDS             1913..>3008
                     /locus_tag="PLN77_09715"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRM44580.1"
                     /translation="MKLEEIGTRARQAEPVLRIMETEKKNRVLKKAAENLVADAETII
                     AANALDMEHGKEKGMSQGLLDRLFLDKSRIEAMAEGMCQVSELDDPIGEVLGMKKRPN
                     GLLIGQKRVPLGVVGIIYEARPNVTADAFALCFKTGNVVILKGGSDAIHSNTAIVASL
                     RKTLKEEQVPEDALLLIEDTSRETTAQFMKMNRYVDVLIPRGGAGLIRAVVENSTIPV
                     IETGTGNCHIYVDETADLSMAVDIILNAKTQRVGVCNACESIVVHENVREKLLPVLEE
                     RLKEKHVEMRADEKAREYMPEAEEATEEDWGKEYLDYIVSIKTVSSIDEAIAHINHYN
                     TKHSEAIITNNYTHAQKFLDEIDAAVVYVNA"
BASE COUNT          866 a          652 c          878 g          612 t
ORIGIN      
        1 agctacggaa ccaccggccc cgaccgaaga tccggcaccg gagccgctgg ttccggaagc
       61 gattgagccg acacccgcgg aggatgcgct gccggaaggt atggtgaaaa gttatctgac
      121 aggagaacct gtcgccgaag tacagggaag aaggcgcccg gtggcggtta tgctgagtaa
      181 tatcatcagc gcctgcccgc agagcggtat cagccgtgcg ggagtgatct atgaagcgcc
      241 ggtcgaaggc ggcattacgc gcctgatggg gctgtttgaa aactatgatg atctcgaaaa
      301 gatcggatcg gtgcggagct gccgcgaata ttatgtgtat ctggcagcgg gatttgacgc
      361 tctgtattac cactatggac aggcggcgta tgcgattccg ctgctcgaat cggatcacat
      421 agacaatgtg aacggcatgg agtattcgga acaggtgtat taccggacgt cggatcgtcc
      481 gtcgccgcac aatgcgtatt tggatgcggc cgggattgca gtgggaatcg atctctgcgg
      541 ctaccggaca tcttacaaag acggttatga cggatttttc cagtttgcat ccgagacaga
      601 accggaaacg ctggaacagg gacgaaatgc cacctatgtg gcaccgggcg gatatttcca
      661 caataatcca tggttttcgt acgatgaggt gagcggcacg tatttgcgtt atcagttcgg
      721 ggaaccgcag atcgatgaga tgacacagga acagcttgcg gtgaagaaca ttatcgtgca
      781 gtactgttcc tggaaaaagt atgatgacaa tgcggaatat ctcgatatcg atgtggtaaa
      841 cggcggaacg ggaacgtata tcacaagcgg aaaggccatc gacgtgacat ggagcaagga
      901 tgatccgtgg ggcgtcacaa aataccggga tgggaatggc gatgagatta agctgaatcc
      961 tggaaaaaca tgggttctga ttgtgctgga taattatgca gacgagactg aaatccggtg
     1021 attgaaaacc ggatttcaga tttttcgcgg atctgctgga cagataccgt gttttgcggt
     1081 aagataaaga gaaaattccg gtaatttaaa ggagagaaat acagaaaatg gaaattaata
     1141 tacagagaat taacgaactt gcccgcaagg cgaaagcaga ggggctgaca ccggcggaga
     1201 aagaagagca gcagaagctg cggagagaat atattgcagc cgtgaaaatg aacctccgta
     1261 cccagctcga caatattgat atgcaggaga aagacggaac gatcgtaaat ctgggggagc
     1321 gctatggaag caaaaggaaa cattagaaaa gaagtttttg cccgaagaag agcagccaca
     1381 caggaagaaa ttcaggaaaa gagccgtatg atttatgaaa aaatcacggc tctgccggag
     1441 tttctggatg cggactgctt ttttgcgtat atggacttca aaaaagaagt catgacacgg
     1501 gatctgatcg agaaggccct gcaaatggga aagacggtcg ctgtgccgcg cgtggaaggc
     1561 gatgatatgg tttattatga gatcaaagat ttctccacgc tgaaatccgg ctattttggc
     1621 attatggagc cggacggcgg aaatgtctgc atgcgggaag agggctttct gctggtaccc
     1681 ggtgtggcct ttgatccggc gcgccacaga gtcggatatg gaaaaggatt ttatgaccgc
     1741 tttcttgccg cacatccagg gtttacaacg gttgcagcgg cgtttgagtt tcagctgttt
     1801 gatgcggtgc cgttcgagga gacggacatt ctgccgcaga tgctggtgac agaacagaag
     1861 atttaccgtt aagccaatta cgataagaca acacgaggag gagacgagta tgatgaagct
     1921 ggaagaaatc ggaacgagag cccgccaggc ggagcctgtg cttcgaatca tggagacgga
     1981 gaagaaaaac cgtgtgctga aaaaagcagc ggaaaacctt gtggcagacg ccgaaacgat
     2041 tatcgcagcc aatgcgctgg atatggagca tggaaaagaa aaaggcatgt cccagggcct
     2101 tctggaccgg ctctttctgg ataaaagccg gattgaagca atggcagaag gcatgtgtca
     2161 ggtatccgag ctggatgacc cgatcgggga agtgctggga atgaaaaagc gcccgaacgg
     2221 tcttctgatc ggacagaaac gtgttccgct tggtgttgtc ggtatcatct acgaggcacg
     2281 cccaaatgtg acggcggatg catttgcact ctgcttcaaa acgggaaatg tcgtgatttt
     2341 aaaaggcgga agcgatgcaa tccactcgaa tacggcgatt gttgcctctc ttcggaaaac
     2401 actgaaagag gagcaggtgc cggaagacgc cctgcttctg atcgaagata cctcccgcga
     2461 gacgacggcg cagtttatga agatgaaccg ctatgtcgat gtcctgatcc cacgcggcgg
     2521 tgccggactg atccgcgctg tcgtggaaaa cagcacgatt cctgtcattg agacgggaac
     2581 cggaaactgt catatctacg tggatgagac agccgatctt tccatggcag tggatattat
     2641 tctgaacgca aaaacacagc gcgtcggcgt atgtaatgca tgtgaatcga ttgtggtaca
     2701 cgaaaatgta cgtgaaaaac tgctcccggt tctggaggaa cgattaaaag aaaaacatgt
     2761 ggaaatgcgc gcagacgaaa aggcaagaga atacatgccg gaagcagaag aagcgacaga
     2821 agaggactgg ggaaaagagt atctcgacta catcgtgtcc atcaaaaccg tctcttccat
     2881 cgacgaagcg attgcacaca tcaaccacta caacacaaaa cactccgaag caatcatcac
     2941 caacaactac acccacgccc agaaattcct ggacgaaatc gacgcagcgg tggtctacgt
     3001 caacgcct
//
LOCUS       DAOMUF010000220         2982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_9452_length_2982_cov_4.297916, whole genome shotgun sequence.
ACCESSION   DAOMUF010000220 DAOMUF010000000
VERSION     DAOMUF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9452_length_2982_cov_4.297916"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PLN77_09720"
     CDS             <1..1287
                     /locus_tag="PLN77_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRM44581.1"
                     /translation="LCFRKPSNMEIYSRDGFVCVSLSWVVLGVFGAVPFVITGEIPFY
                     INALFEIVSGFTTTGASILSDVEALSHASLFWRSFSHWIGGMGVLVFILALLPMKGGS
                     VMNLMKAESPGPTVSKFVPRVRGTAKILYQIYLGMTLFTIAALLLSGMKTFDSVTLAM
                     GAAGTGGFAVLNSGCAVYTPLQQWILTIAMIAFGVNFNFYYLMLCKKGKAAISSQEVR
                     AYFLIILLAGGVISWNTYPMYHSIGETVRTAFFQVGSIITTTGYSTTDFDLWPELSKC
                     ILVMLMFIGACAGSTGGGIKVSRILILLKTVKKELGSIVHPRSIKRIRFDDHPVEHEV
                     LRAINVFLIVYLVIYSFSMLIISVDGFSFETNFTAVAATLNNIGPGLDQVGPTSNFFG
                     YSPVSKLILIFDMLAGRLELFPMLILFYPSTWKKRG"
     gene            1450..2529
                     /gene="hisC"
                     /locus_tag="PLN77_09725"
     CDS             1450..2529
                     /gene="hisC"
                     /locus_tag="PLN77_09725"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRM44582.1"
                     /translation="MSNWEKNVRKVVPYVPGEQPHEADMIKLNTNENPYPPSPKAAEA
                     IRRVAEEDTLRLYPDPTAAELVQAIADVYHVSPKQVFVGVGSDDVLAMAFMTYFNSDK
                     PILFPDITYSFYDVWAEEFRIPYEQIPLDENFCIVPEQYFAENHPNGGIVFPNPNAPT
                     GVELSLSAIESILQHNPDVVVIVDEAYVDFGAATAQPLLEKYENLLVVQTTSKSRSLA
                     GMRIGYAIGNEKMISYLNDVKYSFNSYTMNQTTIAAGAAAIRDVEYFRKTVKKVMETR
                     EWSKEELKKLGFSFEDSKSNFIFATHKSVPAGKIFQALRERHIYVRYFEKPRIDNYLR
                     ITIGTREEMQTLFDFLKDYLTESSK"
     gene            2690..>2982
                     /locus_tag="PLN77_09730"
     CDS             2690..>2982
                     /locus_tag="PLN77_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44583.1"
                     /translation="MNKEKKEVVKVNQITEGVIWQQILIFFLPILFGTFFQQLYNTAD
                     AMIVGHFLGKQALAAVGGTTGTLINLLVGFFVGLSSGATVVISQHYGANKEDKV"
BASE COUNT          770 a          648 c          798 g          766 t
ORIGIN      
        1 ctctgcttca gaaaaccgtc caatatggaa atctattccc gcgacggatt tgtctgcgtt
       61 tcgttaagct gggttgtcct tggtgttttc ggagcggttc cgtttgtaat tacgggggaa
      121 attccgtttt atatcaatgc gctgtttgag atcgtttccg gttttacaac gacaggagcc
      181 agcattttga gcgatgtgga agcactttca cacgccagcc tgttctggag aagcttttcc
      241 cactggattg gcggtatggg cgttcttgtg ttcattcttg cattgcttcc gatgaagggc
      301 ggttccgtca tgaacctgat gaaggcagag agcccgggac cgactgtttc caaatttgtt
      361 ccgagagtgc gcggaaccgc taaaattctg tatcagattt atcttggcat gacacttttt
      421 accattgctg ccctgctgct ttccggcatg aagacatttg attctgtcac actcgccatg
      481 ggagctgccg gaacaggagg ctttgccgtg ttgaacagcg gttgtgcagt gtacacacca
      541 ctgcagcagt ggattctgac aatcgccatg attgcgttcg gtgtgaattt taacttttat
      601 tatctgatgc tttgcaaaaa gggaaaggcc gccatttcgt cccaggaggt aagagcgtat
      661 ttcctgatta tccttctggc aggaggcgtg atttcctgga atacctatcc gatgtaccat
      721 tccatcggtg agacagtacg gaccgccttt ttccaggtcg gatcgattat cacgacaacg
      781 ggttattcca cgacggactt tgacctctgg ccggagctgt caaaatgtat tctggtgatg
      841 ctgatgttca tcggagcctg cgccggttca acgggaggcg gaatcaaggt ttcgcgaatc
      901 ctgatcctct taaaaaccgt gaaaaaagag cttggttcga tcgtacatcc gcgcagtatc
      961 aaacgcatcc gtttcgacga tcacccggta gagcatgagg ttctgcgggc gatcaacgta
     1021 tttttgattg tttatctggt gatctacagc ttttccatgc tgatcatcag tgtggatgga
     1081 ttttccttcg agacaaactt cacggcagtt gccgcaacac tgaacaacat cggaccgggt
     1141 cttgaccagg taggaccgac cagtaacttc ttcggataca gtccggtttc caagctgatt
     1201 ctgattttcg atatgctggc aggccgtctg gaactgtttc cgatgctgat ccttttctat
     1261 ccgtctacct ggaagaagag aggataaagc ggttcaccgg gaagaagagg gactttgcgt
     1321 cggcgggaaa ctgccgggag cagaaggaaa agaaaaaatg gttacgattc acagggtttt
     1381 ccaaaaaaac agaaagacgg aataaagtca gggtgcattt cctgaccaca ggcagaaagg
     1441 aaggaactta tgagtaactg ggaaaagaat gtaagaaaag ttgtgccgta tgtaccgggc
     1501 gagcagccgc atgaggctga tatgatcaag ctgaatacga atgagaatcc gtatccgccg
     1561 tcaccaaaag ccgcagaggc gatcagacgg gtagcagaag aggataccct ccgtctctat
     1621 ccggatccga ccgcggcaga actggtgcag gcgatcgcgg atgtctacca cgtaagcccg
     1681 aagcaggttt ttgtcggtgt cggttcggat gatgttctgg cgatggcgtt tatgacgtat
     1741 tttaactctg acaaaccgat tcttttcccg gacattacgt attccttcta cgatgtctgg
     1801 gcggaggaat tccgtattcc gtacgagcag atcccgctcg atgaaaattt ctgcattgtg
     1861 ccggagcagt attttgcaga gaaccatcca aatggcggaa ttgtgttccc gaacccgaat
     1921 gcaccgacgg gtgtggagct ttcgctttct gcgatcgaat cgatattgca gcataacccg
     1981 gatgtggttg tgatcgtaga tgaggcatat gtggatttcg gagcagccac tgcgcagccg
     2041 ctgctggaaa aatatgaaaa cctgctggtt gtgcagacaa catcaaaatc ccgttcgctt
     2101 gccggcatgc gcatcggcta tgcaatcgga aatgagaaga tgatttcgta tctgaacgat
     2161 gtgaaatatt cattcaattc gtataccatg aatcagacca cgatcgcagc cggagccgca
     2221 gcgatccgcg atgtggaata tttccgcaag acggtgaaaa aagtgatgga aacgagagag
     2281 tggtcgaagg aggagctgaa aaagctggga ttttctttcg aagattcgaa atcgaacttt
     2341 attttcgcaa cacacaaaag cgtaccggcc gggaagattt ttcaggcact ccgggaacgc
     2401 catatttatg tgcggtattt tgagaaaccc cgcattgaca attatctgcg tattacgatt
     2461 ggcacgcggg aagaaatgca gacgctgttt gattttttga aagattactt aaccgaatca
     2521 agcaagtagc gaagcggatt gcaaatatcc gcttgccaag agggatgaag cagctgaaaa
     2581 tataaaacag aagaagagta tctgcagagg agcagaagaa aattttaaag ggggactgcc
     2641 gtcggaatca gccatgagat ccggcggcag ttttagaagg tatttgggta tgaacaaaga
     2701 gaagaaagaa gtagtaaagg taaatcagat tacggaaggt gttatctggc agcagattct
     2761 gatctttttc ctgccgattc tgttcggaac gtttttccaa cagctttaca acacggcaga
     2821 tgccatgatt gtcggacact ttctcggaaa acaggcactt gcggcggtcg gcggaacgac
     2881 aggtacgctg atcaatctgc tggtcggatt tttcgtcgga ctttccagtg gtgcgacagt
     2941 tgttatttca cagcattacg gagccaataa agaggacaag gt
//
LOCUS       DAOMUF010000221         2962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_9534_length_2962_cov_3.315789, whole genome shotgun sequence.
ACCESSION   DAOMUF010000221 DAOMUF010000000
VERSION     DAOMUF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9534_length_2962_cov_3.315789"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    254..263
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<264..1191)
                     /locus_tag="PLN77_09735"
     CDS             complement(<264..1191)
                     /locus_tag="PLN77_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRM44584.1"
                     /translation="MIDQKRENLLNLALAATEVERRRVPELSAGYNSYSKTWEVIVQY
                     QGDLAFLEGQGVRVTLLLISYAILLVPESLMDYVTGLPQITYLEKPKLLYFADAFVRS
                     ISCITPVQEGVMGLSGNGVLLACIDSGVDYAHPDFCAPDGTSRIALLWDQTIPGNPPT
                     GYALGSVYTRRQINEALAASSPTERFALVPSRDVTGHGTAVLGIAAGNGRSSADGAIR
                     GVAPEATLVVVKLGNPDPADLPRTSQLLQAVDFCVRYALLVERPLVINLSFGNNYGSH
                     SGQSLLETYLNAVSNLGQHVICIGAGNEGDTGR"
     gene            1306..2736
                     /gene="trkA"
                     /locus_tag="PLN77_09740"
     CDS             1306..2736
                     /gene="trkA"
                     /locus_tag="PLN77_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRM44585.1"
                     /translation="MVLRCRLAGLYYFTRNAFALKGENMKIIIVGCGKVGTALTAQLS
                     REDNLVTVIDTDSIVVRNVSNTYDVMGIVGNGASYQVLQEADIEHADLMIAVTKSDEM
                     NLLCCVIAKQAADCHTIARVRNPMYREEREFIRKKLGLSMIINPEHAAAMEMARLLRF
                     PSAIEIDSFSRGRIEMLRFKVPETSKIVHMSLRELAGALQYSLLVCAVERNGEVYIPD
                     GNFVIQAKDSLSIITTPQNAESLFKKIGVHTNKVHNTMIVGGGEITYYLAKSLANMNV
                     DVKIIEKNKDRCEVLSEALPDATVIYGDGSDQELLKEERIQNMDSFVACTDMDEENII
                     LSLYAKEKVSSKVVTKINHLEFNDVIHSLNLDSLIYPKHITAEYILQYVRAMKNSIGS
                     NVETLYKLMEDRVEALEFIVNQNCRMIGIRLQDMKTRPNLLVAAINRQGKVIIPGGQD
                     SFEAGDSVIIVTTETGLQDIHEILAE"
     gene            2752..>2962
                     /locus_tag="PLN77_09745"
     CDS             2752..>2962
                     /locus_tag="PLN77_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRM44586.1"
                     /translation="MNYKMIRFVISWVLKVEGMLMLLPALVGAMYQEKTGFAYLIWGL
                     LCIAAGLILCFRKPSNMEIYSRDGFV"
BASE COUNT          771 a          754 c          756 g          671 t
ORIGIN      
        1 tcgagatttc atccccataa accttccaga tctggagact gaacgaggat tcataggcgc
       61 cgacctgaaa ttcaatggag acagcgcggt tggccgctgc cgagactgca ggaggattgg
      121 gcagtgcgga cattgtgtgg accattcgag cggtagctgc cgagaccgca gacgtcgcag
      181 tcggatcgga cgtcgcgcgg acggtttgcg tttggccggc cgacctgcga tccgacttca
      241 ggttcccggc atannnnnnn nnnggcgtcc ggtatcccct tcattccctg ccccgataca
      301 gatcacatgt tgccctaaat ttgacactgc attgagatac gtttccagca gcgattgccc
      361 gctgtgcgaa ccataattat tcccgaaact caagttaatg acgagcggcc gctccaccag
      421 cagcgcatac cgtacacaga aatccaccgc ctgcagcaat tgcgatgtcc gcggcagatc
      481 cgccggatca ggatttccaa gctttacgac aacgagcgtt gcctcaggcg ctacaccccg
      541 tatggctcca tccgcagagg accgcccgtt tccggcggca attccgagaa ccgctgtccc
      601 atgccccgtc acatcgcggg acggcacgag ggcaaaccgc tccgtgggcg aagaagccgc
      661 aagtgcctca ttgatctgcc gccgtgtata caccgatccg agtgcatacc ccgtcggtgg
      721 atttcccgga atcgtctgat cccataaaag tgcaatccgg cttgtcccgt ccggtgcgca
      781 gaaatccgga tgcgcatagt ccacgcctga atcaatgcat gcaagcagca ccccattccc
      841 agacagaccc ataaccccct cctgcacagg cgtaatacag gatatgctcc gcacaaatgc
      901 atctgcgaaa taaagtaatt tcggtttctc cagataggta atctgcggca gtcctgtcac
      961 gtaatccatc agggactccg gaaccagaag aatggcatac gagatcagca gcagcgtcac
     1021 acgaactccc tgcccctcca gaaaagccag gtctccctga tactgtacaa ttacttccca
     1081 tgttttgctg taagagttat aacctgccga aagctccggc acacgccgcc gttcgacttc
     1141 cgttgcggcc agcgcaaggt tcaacaggtt ttcccttttt tgatcaatca ttacaaaaac
     1201 tccgtccaaa atgttgttaa aatctattcc gcttttcctg gaaatatgat ataatcgcca
     1261 tcgtacaata ggggtacaga tctgcttttt ggagtgcatc gaaaaatggt tttacgatgc
     1321 cgcctggcgg gtctttacta ttttacgcga aatgcttttg cattgaaggg agagaacatg
     1381 aaaattatca ttgtgggctg cggaaaagtc ggaaccgcac tgaccgccca gctgagccgc
     1441 gaggacaacc ttgtgacggt cattgacaca gacagtattg tcgtccgcaa cgtctccaac
     1501 acctacgacg ttatgggaat tgtaggaaac ggagcaagct atcaggtgct tcaggaggcg
     1561 gatattgagc atgcggatct gatgattgcc gtaaccaaaa gtgatgaaat gaacctgctt
     1621 tgctgtgtca tcgccaaaca ggcagcagac tgtcacacca tcgccagagt tcgaaatccg
     1681 atgtatcggg aggagagaga atttatccgt aaaaagctgg gtctgtccat gatcatcaac
     1741 ccggagcacg cggccgccat ggaaatggca agactgctgc ggttcccatc tgcgattgag
     1801 atcgacagct tttcccgcgg aagaatcgaa atgctgcgct ttaaagtgcc ggaaacatcg
     1861 aaaattgtcc atatgtcgct gcgtgagctg gccggagcgc ttcagtacag tctgctggtc
     1921 tgtgctgttg agcgaaacgg cgaggtttac ataccggatg gaaattttgt gatccaggca
     1981 aaagacagcc tttccatcat taccacaccg cagaacgcag agagcctgtt taagaaaatc
     2041 ggtgttcata caaacaaggt acacaatacg atgatcgtcg gcggcggtga aattacgtat
     2101 tatctcgcaa aatcactggc aaatatgaac gtcgatgtca agattatcga gaaaaataag
     2161 gatcgctgtg aggttttaag cgaagcactg ccggatgcaa ccgttattta cggtgacgga
     2221 agtgaccagg agctcttaaa agaggagcgc atccagaata tggattcctt tgtggcctgc
     2281 accgatatgg atgaggaaaa catcattctg tcgctgtatg cgaaagagaa ggtatcctca
     2341 aaggttgtaa cgaaaatcaa ccatctggaa tttaatgatg ttatccacag cctgaacctg
     2401 gacagcctga tctacccgaa acacatcacc gcagagtaca ttctgcagta tgtccgggca
     2461 atgaaaaact ccatcggaag caacgttgag acgctgtata agctgatgga agaccgtgtg
     2521 gaggctcttg agtttatcgt gaaccagaat tgccgtatga tcggcatccg tctgcaggat
     2581 atgaaaacac ggccaaacct tctggtagca gcgattaaca gacagggaaa ggtcatcatt
     2641 ccgggtggac aggattcctt tgaggcagga gattccgtca ttatcgtaac aacggaaacc
     2701 ggtcttcagg atatccatga gattctggca gagtaacggg ggaagcggat tatgaactat
     2761 aaaatgattc gttttgtcat cagctgggtg ctgaaagtgg aaggtatgct gatgctgctt
     2821 ccggcgctgg tcggagccat gtatcaggaa aagacaggct ttgcgtatct tatctgggga
     2881 ctgctctgca ttgcggccgg tctgattctc tgcttcagaa aaccgtccaa tatggaaatc
     2941 tattcccgcg acggatttgt ct
//
LOCUS       DAOMUF010000222         2959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_9547_length_2959_cov_3.981061, whole genome shotgun sequence.
ACCESSION   DAOMUF010000222 DAOMUF010000000
VERSION     DAOMUF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9547_length_2959_cov_3.981061"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1854)
                     /locus_tag="PLN77_09750"
     CDS             complement(133..1854)
                     /locus_tag="PLN77_09750"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44587.1"
                     /translation="MRNLWILTRFLLKSGPGKSNQKSGKKKAFSDGGRIVVYLLLGVC
                     LLPVSVLLFLLGYSGYTFLQPLGLETLLIEFVCAIGMMVIFFYGLPLFLSEYYMDSDL
                     VKLLPLPFTPAQIVGAKGFCCLLNEYYLIVLLFFPFLLGYGISAKMGIFYWINMILAC
                     LIFPVVPLVYAAVLTMLVMRLFKNIRNKDFLSYLGFAASLIFAIGINVFSRSIGNFEM
                     QDIMNLMESQKGTLRAFRTIFPNLPLMTGSLADASFLKMILYIATTAVILAVFFALAW
                     KIYLPAVLGMSETTSEKRILSKEEVTRTVKSKNPVRTYAMIEWKKLYRTPAWFMNCVL
                     MPLIWPVFMLGIALISIISSLGMAKTTGLWTRLVADGTIFRLLKGELPVAVAVLTAAG
                     IAVMMSMFCVISATAMSRKGSEYIYMKCIPMSYHDQIRAMLVSGILISLLGTLPYALV
                     FNIIAVVFGLHPATLLYTTAITVLFTLFVNYEQLLFDLAFPKLNWENETAAIKSNNRS
                     LISVLIDLTVGAILIGAGYLLYGKLHLNIHITTSVMILLTAVLTFAMRTALFKWGVQV
                     MEHLESA"
     gene            complement(1847..2572)
                     /locus_tag="PLN77_09755"
     CDS             complement(1847..2572)
                     /locus_tag="PLN77_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44588.1"
                     /translation="MIRLNHVSKIYGDGSKKAVDDLSWDIEDGKITGFIGPNGAGKTT
                     TLKMITGILAPSEGTIEINGKDIQKDPLEAKRQFGFVPDDPDAFLRVRGIEYLNFMAD
                     IYGVGTEERQHFIEETAKRFEMEENLSSRIMSYSHGMRQKIMVMGALIHKPPVWILDE
                     PLTGLDPRSAHELKQMMREHADAGNAVLFSTHVLEVAEKLCDRIAIIHHGRQVYLGTL
                     EELKAAYPDQTLENIFLEITEHA"
BASE COUNT          753 a          687 c          777 g          742 t
ORIGIN      
        1 ccgtacagag ccctggcgcc gatcacaagg tacgcagcga aattggaaag gagcgcaaaa
       61 aaaagagatc cgcacattgg ctgaaatctc ttttctatca cattttaata ttgttattat
      121 gtctcgaaac tgttaagcgc tttccagatg ttccatcact tgaacgcccc atttaaaaag
      181 ggcggttcgc atggcgaagg tcaggactgc ggtaagcaga atcatcacgc tcgtggtgat
      241 gtggatgttg aggtgaagtt ttccgtacag caggtagccg gctccgatca ggattgcgcc
      301 gacggtgagg tcgatgagga cggagatcag ggagcggttg ttggatttta tggcggcggt
      361 ttcgttttcc cagtttaatt tcggaaatgc aaggtcaaaa aggagctgtt catagttgac
      421 gaacagggta aataaaaccg tgatcgccgt ggtgtaaaga agggtcgccg ggtgcagacc
      481 gaaaacgacc gcgatgatgt tgaagaccag agcgtacggc aaggtgccaa ggagactgat
      541 caggatgccg cttaccagca tggcacggat ctggtcgtga tagctcatcg gaatgcattt
      601 catatagatg tattcgctgc cctttcggga catcgctgtg gcggagatca cgcagaacat
      661 ggacatcatg acggcgattc cggcggcagt aaggacggca actgcgacgg gcagttcccc
      721 tttcaggagg cggaatatag tgccatcggc gacaagccgg gtccaaaggc cggtagtttt
      781 agccatgcca aggctggaaa tgattgaaat gagggcaata ccaagcatga agaccggcca
      841 gatgagaggc atcagaacac aattcatgaa ccaggccggt gtgcggtaca gttttttcca
      901 ttcaatcatc gcgtaggtgc ggaccggatt tttgcttttg acggttcggg tcacttcttc
      961 tttggataaa atgcgttttt cgctggtggt ttcgctcatg ccgaggacag ccgggaggta
     1021 gattttccag gcgagggcga aaaagacagc aaggatgacg gctgttgtag cgatgtagag
     1081 tatcattttg agaaaggatg catctgcaag gcttccggtc atcaggggca ggttcggaaa
     1141 aatggtccgg aaggcgcgca gagtgccctt ctggctttcc atcaggttca tgatatcctg
     1201 catttcaaaa tttccgatac tgcgcgaaaa tacgttgatg ccgatcgcaa aaatcaggga
     1261 tgcggcgaat ccgaggtagc ttaagaaatc cttgtttcgg atatttttga aaagacgcat
     1321 cacgagcatc gtcaaaactg cggcgtaaac gagcggcaca accgggaaaa taaggcaggc
     1381 gaggatcata ttgatccagt aaaaaatgcc cattttggcg ctgatcccgt accctaacag
     1441 aaacggaaag aacagcagta caataagata gtattcgttc agcagacagc agaagccttt
     1501 cgcaccgaca atctgcgccg gggtaaacgg cagcggcaaa agctttacga ggtcagagtc
     1561 catgtaatac tcgctcagaa acagcggcag gccatagaag aaaatgacca tcatgccgat
     1621 ggcacagacg aactcgatca gcagggtttc cagcccgagc ggctgcagga aggtatagcc
     1681 ggaatatccc agaagaaaca gtaagaccga cacgggaagg aggcagacac caagcagaag
     1741 gtatacaacg atacgtcctc cgtccgaaaa tgctttcttt tttccgctct tctgattgct
     1801 ttttcctggt ccgcttttca gaaggaaacg ggtgagaatc cataagttac gcatgttcgg
     1861 tgatctccag aaagatattt tcaagtgtct gatccggata ggccgctttt aattcttcca
     1921 gggtacccag gtatacctgg cggccgtgat ggatgatggc gatgcggtcg cagagttttt
     1981 ccgctacttc cagcacatgg gtggaaaaca gcacggcgtt tccggcatcg gcgtgttcac
     2041 gcatcatctg tttgagctcg tgcgcggagc gcgggtcaag gccggtcagc ggctcatcga
     2101 gaatccagac cggcggcttg tggatcagtg cgcccatcac catgattttc tgacgcatgc
     2161 catgcgagta gctcatgata cggctcgaaa ggttctcttc catctcaaag cgttttgcgg
     2221 tttcttcgat aaaatgctga cgttcttccg ttccgacacc gtagatatct gccataaaat
     2281 tcagatattc aattcccctg acgcgaagaa acgcatccgg gtcgtctggc acaaaaccga
     2341 actggcgctt ggcttcgagc ggatccttct ggatatcttt tccattgatt tcaatcgttc
     2401 cctcggacgg tgccaggatg ccggtgatca tttttaatgt tgtcgttttc ccggctccgt
     2461 ttgggccgat aaatccggtg atctttccat cttcgatatc ccaggaaaga tcgtcgacgg
     2521 cttttttgct tccgtctccg taaatcttac tgacgtggtt cagacgaatc atgttttatt
     2581 ccccctcttt tttctttgta aagcaaagct atcattctct tgcttcgttt ctaaaaacag
     2641 actgcccttt tctcactccg aaagctctta tccttccctc gcaggaaaag gagtcaggcg
     2701 gatattcgca atccgcttcg ctacttgctt gattcggtta aatctcgaag cacgggctgt
     2761 gatcttgtgt ttttttcagt atgcattttt ctgtcagaac tgtcaatcgt cggagcgaaa
     2821 aatacagaat tttgtaagaa cttgtaagac ttttagagcg tgtctgaaaa aaaagatacc
     2881 gcaaagtgaa gcctgcagat tgcggaaatg ctttttcaga cacgctccag gatcaaatcc
     2941 gattttaccc tctactttt
//
LOCUS       DAOMUF010000223         2957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_9556_length_2957_cov_4.167815, whole genome shotgun sequence.
ACCESSION   DAOMUF010000223 DAOMUF010000000
VERSION     DAOMUF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9556_length_2957_cov_4.167815"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..129
                     /locus_tag="PLN77_09760"
     CDS             4..129
                     /locus_tag="PLN77_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HRM44589.1"
                     /translation="MSQIVLVITGIAMMGYGAVRGEAAAVLGKAIKLCLECVGIG"
     gene            122..1036
                     /locus_tag="PLN77_09765"
     CDS             122..1036
                     /locus_tag="PLN77_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004849719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM44590.1"
                     /translation="MDKKKKLRSKKIAGIRGWIQAAATLLTNPHIPNFFQGKIYQGKA
                     KTVCVPGLNCYSCPAATGACPIGAFQAVVGSSKFKFSYYITGFLILLGVTLGRFICGF
                     LCPFGWFQDLLHKIPGKKFSTARLKPLRYLKYIILVVFVILLPMFATNSIGMGDPFFC
                     KYICPQGVLEGAIPLSIGNAAIRSALGKLFSFKFCILITVVVLSILFYRPFCKWICPL
                     GAIYSLFNKVSFLHITIENSKCVGCNQCSRACKMDIDVCRTPNHPECIRCGACIKACP
                     KDAIHYQFMGKTCQKKDTGDQQSEIRNH"
     gene            1120..2061
                     /locus_tag="PLN77_09770"
     CDS             1120..2061
                     /locus_tag="PLN77_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRM44591.1"
                     /translation="MKTKRTFIPVFTLSVALLLTACNGKEAADTKKTQTTQTESGTEA
                     VTGSVAESGTETAGASGTKLDDLYQQENQIFSDHADVWNKVFGMMNKSTADPSGNYGD
                     YLADTVEANKDSFSEDELNTLTDDIETIRKIEEQMAELENGNTASEDSDAKNDSEEAS
                     PFRDFSGQDYDGNTVDESLFSQNAVTVVNFWFTGCKPCVAELSKLNELNDAIKSMGGE
                     VVGINTETFDGNQDAIKEAAAVLESQGAKYRNLSIDSASAAGKYASDIMAFPTTILVD
                     RNGNIVGEPMLGGIDNQENYDTLMKQIQSVIDADSAK"
     gene            complement(2431..>2957)
                     /locus_tag="PLN77_09775"
     CDS             complement(2431..>2957)
                     /locus_tag="PLN77_09775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HRM44592.1"
                     /translation="LYVIYALLYGIFTMITVALGFRAGFSFSAGAMDLLFSSSLPAAQ
                     KTWLIIPLGIAAFLVFYFVFLFAIKKWDLKTPGREDDDLEAEKKVELASDNYTAIAAK
                     ILEGCGGKENITSIDNCVTRLRLEVKDITAVNDKVIKSAGVAGVIKPGKTSVQVIVGT
                     KVQFVADAFSKLCE"
BASE COUNT          840 a          624 c          727 g          766 t
ORIGIN      
        1 aaaatatctc aaattgtttt agttatcaca ggcatagcaa tgatgggcta tggcgccgtc
       61 agaggggaag cggctgctgt gcttgggaaa gcaattaaat tatgtctgga gtgtgtcgga
      121 attggataag aaaaagaagt tacgatcaaa gaaaatagcc ggaatacgcg ggtggataca
      181 ggcagcggcc acactgctta ccaatcctca tatccccaat ttttttcagg gcaaaatata
      241 tcagggcaag gcgaaaacgg tatgcgtacc gggactaaac tgttattcct gtcctgctgc
      301 gacgggagcg tgccccatcg gagcctttca ggcggttgtc ggatcatcca aatttaagtt
      361 ttcctactac ataaccgggt tcctgatttt actcggcgtg acactgggaa gattcatctg
      421 tggttttttg tgtccgtttg gatggtttca ggatttgctt cacaaaatcc ccggaaagaa
      481 attctctacg gccagactga agccgctgcg gtatctgaaa tatatcatcc ttgttgtttt
      541 tgtgattctt ctgccgatgt ttgcaacgaa ttcgatcggg atgggagatc cgttcttctg
      601 caaatatatt tgtccgcagg gcgttctgga gggggcgatc ccgctttcca tcggaaatgc
      661 cgcaatccgg tctgcactgg gcaagctgtt ttcgtttaaa ttctgtattc tgattacggt
      721 tgtcgtgctc agcatcttgt tttacagacc cttctgcaaa tggatctgtc ctcttggggc
      781 aatctattcc ttatttaata aggtaagctt tttacatatc acgattgaaa acagcaaatg
      841 tgtcggatgc aatcagtgtt ccagggcatg taaaatggat atcgacgtgt gccggacccc
      901 aaatcatccg gaatgcatcc gatgcggagc ctgcatcaag gcctgcccga aggatgcgat
      961 ccactatcag tttatgggaa aaacgtgtca gaaaaaggac accggagatc aacagtcaga
     1021 gatcagaaac cattgattag cagcagatca cgaagcatcg aaggatgata ttgaattttt
     1081 gttttttaca ttatactatc ttaaaaggag ccataattta tgaaaaccaa aagaactttt
     1141 atccctgttt ttacactcag cgtagcatta ttacttaccg catgcaacgg aaaagaggct
     1201 gcagacacaa agaaaacgca gacaacccag acggaatccg gcacagaagc tgttacggga
     1261 tctgttgcgg aatccggaac cgaaacggca ggggcttccg gcacaaagct ggacgattta
     1321 tatcagcagg agaaccagat tttttcagat catgcggatg tatggaacaa ggtgtttggc
     1381 atgatgaata aaagcacggc tgatccgagc ggaaattatg gggattatct ggcagacacg
     1441 gttgaagcca acaaggactc tttttctgag gacgagctga atacactgac ggatgatatt
     1501 gaaacgatac gaaaaatcga ggaacagatg gcggaattgg aaaacggcaa tacagcatcg
     1561 gaagatagcg atgcgaaaaa cgattccgaa gaggcatctc cgttccggga tttttcgggt
     1621 caggattatg atggcaacac agttgacgaa agtctgtttt cccaaaatgc ggtaacggtc
     1681 gtgaatttct ggtttaccgg ctgcaagcct tgtgttgctg agttatccaa gctgaacgaa
     1741 ttaaacgatg caatcaaatc catgggaggc gaggttgtag gcatcaacac agaaaccttc
     1801 gacggaaatc aggatgcgat caaagaagca gcagcggttc ttgaaagcca gggtgcgaaa
     1861 taccgcaatc tttccattga ctctgcttcc gctgcaggaa agtatgcttc cgatattatg
     1921 gcatttccga ccacaatctt agtcgatcga aatggaaata ttgtgggaga accgatgctt
     1981 ggcggaatcg acaatcagga gaattatgat acgctgatga aacagatcca gtctgtgata
     2041 gatgcagaca gcgcgaagta agacgagagc tgcggcgttt tttgggagag aaaatgggag
     2101 tgcgaaaaaa caaagttacg ttttttcgca ctccttttca taacaagatt taaaaaaata
     2161 catttacgaa acgatgtact gtgctgtacc tgtcccgaaa agctgctcgg tgcttagaac
     2221 aagagctgag gtcacacggc gattccccac atatatgcac acgttttcgg aaccaccagt
     2281 aatccgacct ttggttttga tagcagccat ttgacaaaca acagccgggt tcctgctctg
     2341 cgaaagggca gaaaataagg aaaactttgc gcaattaaaa ggagcatacc gcatcagcga
     2401 tatgctcctt ttggactacc tgggtaaata ttattcacaa agtttggaga aagcgtcagc
     2461 tacgaactga acttttgttc caacgataac ctgtacagag gttttgccag gtttgatcac
     2521 gcctgccacg ccggcagatt tgattacctt gtcgtttacg gcagtgatat ctttcacttc
     2581 cagtcgcagt cttgtaacgc agttatcaat gcttgtgatg ttttcttttc cgccgcagcc
     2641 ttccagaatt ttagctgcga ttgcggtata gttatcactt gccagttcta cttttttctc
     2701 tgcttcgaga tcgtcatctt cgcgtccagg agtcttcaga tcccatttct tgatagcaaa
     2761 caggaatacg aagtagaata cgaggaatgc tgcgattcca agcggaatga tcagccatgt
     2821 tttctgtgct gcgggcagtg aggatgagaa caggaggtcc atcgcaccgg cggagaagct
     2881 gaatcctgca cggaagccaa gggcaactgt gatcatagta aagataccat acagtaatgc
     2941 atagattacg tacagag
//
LOCUS       DAOMUF010000224         2923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_9697_length_2923_cov_2.983264, whole genome shotgun sequence.
ACCESSION   DAOMUF010000224 DAOMUF010000000
VERSION     DAOMUF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9697_length_2923_cov_2.983264"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLN77_09780"
     CDS             complement(<1..329)
                     /locus_tag="PLN77_09780"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRM44593.1"
                     /translation="MKVLVTGGAGFIGGNFVHHMVNKYPDYQIVNLDLLTYAGNLETL
                     KPVEDKPNYKFVKGDIADEAFIMDLFEKEKFDVVVNFAAESHVDRSIEDPGIFVQTNV
                     MGTRVLL"
     gene            complement(478..1317)
                     /locus_tag="PLN77_09785"
     CDS             complement(478..1317)
                     /locus_tag="PLN77_09785"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM44594.1"
                     /translation="MGKQEYLSAGRFAALVGTTKETLFHYDEIGLFVPKRRGTNGYRY
                     YAMDQIETFDVIGMLRELGMPLAEIRNYLEQRSPEKFGVLLEQEEKVVREKMQRLREM
                     QRWIGEKKRFLAQASAECREAFEKNPFCPVGIHTLPLQYLVTNHSESADNLAVNQKIG
                     ELYAYCGQYGNRSSYNVGFIQKRETLETGNFLDYRDFYLIFDRVPAKVRYTVRLEGEY
                     LCYYYKGPWQGIEAAYRKLFRYATEQRLLLDDRFYEEYMVDALTSQEMEQYITRIAVK
                     ILR"
     gene            1502..2854
                     /locus_tag="PLN77_09790"
     CDS             1502..2854
                     /locus_tag="PLN77_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44595.1"
                     /translation="MSQSLAKDFNFRSLLKFAFPSIIMMIFMSLYSIVDGFFIAQYVD
                     SMALSAANIVYPAVSILLAVGIMFGTGGSAVVAAKIGQEKQEEANRNFSALTLTTLII
                     GILGAVLGNLFCRQICSLLGATPVLMDYGTAYLRTILSFAPVCLLQALFQSFFVTAGR
                     PGLGLSLTVSAGITNMLLDYLFVVPLHLGVAGAALATGLGQCIPAVAGILYFTFARKG
                     LRFTRPEFSKGLLSTSCFNGSSEMVSNLSAAVVTYLFNMLMLKLEGEIGVAAITAILY
                     GQFLFVALYLGYSIGVAPVFSFNYGSRNKQRLIRLYRISIRFVVVSSVIIALAAAFGS
                     PVISAVFMQKGTYGFELTRHGGYLFSIAYLFCGTNIVASGIFTALSDGKTSALISFLR
                     TFVFIVLSALLLPLVLGTNGVWLSIPVAEFLTLFISVPKLSRYFKDPKVAPETQTSPN
                     "
BASE COUNT          604 a          830 c          611 g          878 t
ORIGIN      
        1 tccagcagta cacgggttcc cattacgttg gtctgaacaa agatgccagg atcctcaatg
       61 gaacggtcta catggctctc tgctgcgaag ttgacaacaa cgtcaaattt ctctttttca
      121 aacagatcca tgatgaaagc ttcgtctgcg atatcacctt ttacaaattt gtagtttggt
      181 ttgtcctcaa ccggtttcag agtctccagg tttcctgcat aggtcagcag gtccaggttt
      241 acgatctgat aatccgggta tttgtttacc atatgatgta cgaagtttcc gccgataaat
      301 ccggctccgc ctgttactaa tactttcatg ctttttctcc tttttggtgt gtttatcgtc
      361 tcagttttat gtcaattcaa cttgttgtac tctcactata atgggtgacg ttgcgtcatg
      421 gtcaagagga gaatgaaaaa agtttgtgaa aatcttgttt tttcccctcc tacctgtcta
      481 tcttaaaatt tttacggcga ttctcgtaat atactgttcc atttcctgac tggtcagcgc
      541 gtccaccata tattcctcat aaaagcggtc gtccagcaaa agtctctgtt ccgtggcata
      601 ccggaacagc ttccggtacg ccgcttcaat tccctgccat ggtcctttat aataataaca
      661 aagatactct ccctccagcc gaaccgtgta tcgcactttc gccggcacac gatcaaaaat
      721 cagataaaaa tcacggtaat ccagaaaatt ccccgtttcc agtgtttccc gtttctgaat
      781 aaacccaaca ttatacgaac ttcgatttcc gtattgccca cagtaagcat agagctctcc
      841 gattttctga ttgactgcca gattatccgc tgactcgctg tgattcgtga caagatactg
      901 caacggcagg gtatgaatcc ccacaggaca gaagggattt ttttcaaacg cctcacggca
      961 ttccgccgat gcctgtgcca gaaaccgctt cttttcccca atccagcgct gcatctcgcg
     1021 gagccgctgc attttttcgc gcaccacctt ctcctcctgt tccagcagta ctccgaattt
     1081 ttcaggactt cgctgttcca gataatttcg aatctctgcc agcggcatgc ccagctcccg
     1141 cagcattccg atcacatcaa acgtttcaat ctgatccatc gcataatacc ggtagccgtt
     1201 tgtccctctt ctttttggta cgaacagccc aatctcatca taatggaaga gcgtttcctt
     1261 tgtcgtgcct accagcgctg caaatcgccc cgcgcttaaa tactcctgtt ttcccatttc
     1321 tgactccttt tcctgaatat gtgctctcgg aatttttcct gtttttcacc ttttttgcac
     1381 ttgactatag ggttacccta tcctttataa tagcaaacga ttcaaatttt cacaagaaat
     1441 ctttcccatc taaagcgtgt ctgaaaaagg cttttcacac ataaaaagga ggtttttgat
     1501 tatgagccag tcactcgcca aggatttcaa cttccgctcc ctgctgaagt ttgcgtttcc
     1561 atcgatcatc atgatgatct ttatgtctct ctacagtatt gtggatggct ttttcatcgc
     1621 acagtacgtg gacagcatgg cgctttccgc cgccaatatc gtctatcctg ccgtcagcat
     1681 tcttcttgcc gtcggcatca tgttcggtac cggaggcagc gccgttgtcg ccgcaaagat
     1741 cggacaggaa aaacaggagg aagccaaccg taacttttcg gcccttaccc ttaccacact
     1801 gatcatcggt attctgggcg cagtgctcgg caacctgttt tgccgtcaga tctgcagtct
     1861 gctcggtgca acgccggtgc tgatggatta cggtaccgcc tatctgcgga cgatcctttc
     1921 ctttgccccg gtctgtcttc tccaggcgct gttccagtcc ttctttgtaa cggcaggacg
     1981 cccgggactc ggactttccc ttacggtgtc cgccggcatt accaacatgc ttctggacta
     2041 tctgtttgtc gttccgctcc atcttggtgt tgccggagct gcccttgcca ccggccttgg
     2101 ccagtgcatt ccggccgttg ccggcatttt atactttacc tttgcccgca aaggccttcg
     2161 cttcacccgt ccggaatttt ccaaaggcct gctctccaca agctgcttca acggttcctc
     2221 cgagatggtc agcaatcttt ctgcagccgt tgtcacttac cttttcaaca tgctgatgct
     2281 gaaattggag ggcgaaatcg gtgttgccgc catcacggcg attctgtacg gacagtttct
     2341 ctttgttgcg ctgtacctcg gctacagtat cggcgttgca cctgtgttca gcttcaacta
     2401 cggcagccgg aacaaacagc ggctgatccg gctttaccgc atcagtatcc gcttcgttgt
     2461 ggtaagctct gtgatcattg cacttgcggc tgccttcggt tctcctgtta tttccgccgt
     2521 tttcatgcag aaaggcacct atggctttga actgacccgc cacggtggtt atctgttctc
     2581 catcgcctat ctgttctgcg gtacgaatat cgtcgcctcc ggcattttta cggcactgtc
     2641 agacggaaag acctctgctt tgatttcctt tttgcgaacc ttcgtcttta tcgttctctc
     2701 cgcgctgctt ctgccgctcg tgcttggcac caacggtgtg tggctgtcga ttccggtcgc
     2761 cgaatttctg acgctgttca tctccgtgcc gaagctgtcc cgctatttca aagatccgaa
     2821 agtcgcaccg gagacgcaaa cgagtcccaa ctgatctgac cgacttcgct gattctcaaa
     2881 accccgcaca atagatccgt tgttttcatc ggatccgccg tgc
//
LOCUS       DAOMUF010000225         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_9924_length_2874_cov_2.904221, whole genome shotgun sequence.
ACCESSION   DAOMUF010000225 DAOMUF010000000
VERSION     DAOMUF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9924_length_2874_cov_2.904221"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1237)
                     /gene="iadA"
                     /locus_tag="PLN77_09795"
     CDS             complement(89..1237)
                     /gene="iadA"
                     /locus_tag="PLN77_09795"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HRM44596.1"
                     /translation="MILFQNIDVYAPEYLGRKDVLTVYDKIVKIADAGTITADGLLSG
                     AETVDGTGLVLTPGFVDCHVHVLGGGGEGGFANRTPEATMEGLNKFGVTTVVGCLGTD
                     GIGRDMCALVAKTKGLREQGMSAWCYTGSYQVPVRTLTDGITKDIMMIEEIIGTGEIA
                     ISDHRSSQPTFEEFARLAADTRLGGVLSGKAGVINVHLGDGARCMELIERVISETEIP
                     ASQFLPTHVNRNEKLFQKAIEYAVNGGAVDFTGNEEIDYWETVCDEVRVCKGIRRMLD
                     AGVNPKHITISSDGQGSLPIFNEKGEFLRMGMGQSSCLLKEVKECVEKAGIPLETAIS
                     TITANPAEILSLKGKGKVREGYDADLCVLDQGLEIQEVIARGKNVYRK"
     gene            complement(1253..2773)
                     /gene="yfcC"
                     /locus_tag="PLN77_09800"
     CDS             complement(1253..2773)
                     /gene="yfcC"
                     /locus_tag="PLN77_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HRM44597.1"
                     /translation="MSKKKGEGLKSFNRGFNVPDTYIIIFFVVVIAAVMTFLVPKGYY
                     ETEDVTYLMNGVEKTRTVIKDGSFQYLTDAAGKPVTEGVALFSGDGGTGFFNYMYNGI
                     ISSSAIEIIAFLMVVGGAFGIMIRTGAVEAGLIGLIRKAKGAEKLLIPVLFVLFSLGG
                     AVFGMGEEALPFTMILCPLFVAVGYDTVIAVLVTYVATQIGFGSSWMNPFSVGIAQGI
                     AGVDVFSGAGFRMVMWVVFTALGCGMTMFYAAKVKKTPEISVAYESDQYFRDQNEKTG
                     IDEGHSFGIGHILVLVTLAVTVVWVIWGVMAKGYYMAEIATQFFIMGIVAGVIGVIFH
                     LNDMKVNDIAVSFKDGAKDLIGAALVVAMAQGIMQVLGGSDPTTPTVINTIMYGISQA
                     LAGLSGAAAAVLMYVFQSVFNFFVVSGSGQAAITMPIMAPLADLLGVSRQTSVLAFQL
                     GDAFTNLIVPTSGCLIGSLAIAKIEWSNWIKFMWKFLGILMIGAVITILIAVAIGF"
BASE COUNT          663 a          802 c          703 g          706 t
ORIGIN      
        1 ctcgtaattt ttctcccatc ccattttttt ccaaaaagaa aaaggctaca gctccacgca
       61 agccgtaacc tcttctctaa tctctcttct atttccgata cacattcttc ccccgtgcaa
      121 tcacttcctg aatctctagt ccctgatcca gtacgcaaag atccgcatca tatccttccc
      181 ggaccttgcc ttttcctttc agtgacagga tctctgccgg gtttgccgta atcgtcgaaa
      241 ttgcggtttc cagcggaatt cccgctttct ccacgcattc ttttacttct ttcagcaaac
      301 agctcgactg gcccatgccc atccgcagga attctccctt ctcattaaag atcggcagac
      361 tgccctgacc gtcggatgag atggtgatgt gtttcgggtt cacgcctgca tccagcatgc
      421 ggcggattcc tttgcacacg cggacttcat cacagacagt ttcccagtaa tcgatctctt
      481 catttccggt gaaatcgacc gcaccgccat ttacggcgta ttcaattgcc ttctggaaca
      541 gcttctcgtt ccggttgaca tgggtcggca ggaactggct ggccgggatt tcggtttcgc
      601 tgatgacgcg ttcgatcagc tccatacatc ttgcaccgtc gccaaggtgg acattgatca
      661 ctcccgcttt cccggaaaga acgccgccga ggcgcgtgtc tgctgccagt cgggcgaatt
      721 cttcgaaggt cggctgtgag gatcggtggt ccgagatggc gatttcgccg gttccgatga
      781 tttcttcgat catcatgata tcttttgtaa ttccatccgt cagtgtgcgc accggaacct
      841 ggtagctgcc ggtgtagcac caggcggaca tgccctgctc ccggagacct tttgtttttg
      901 ccacgagcgc gcacatgtca cggccaatgc cgtcagttcc gaggcagccg acaacggttg
      961 tcacgccgaa tttgttcagg ccttccattg ttgcctccgg ggtgcggttg gcaaagccgc
     1021 cctcaccgcc tccgccgagc acgtgaacat ggcagtcgac aaagccaggt gtcaggacaa
     1081 gtccggttcc gtctacggtc tccgcgccag agagcaggcc gtctgccgtt atcgtgccgg
     1141 catctgcgat cttgacaatt ttatcataca ccgtaagaac gtcttttctt ccgaggtatt
     1201 ccggcgcata gacgtctatg ttctgaaata atatcatttt ctctcactcc tcttaaaatc
     1261 caatggcaac cgcaatcaaa atcgtaatga ctgctccaat catcagtatc cctaagaatt
     1321 tccacatgaa tttgatccag ttcgaccact cgatctttgc aatcgccagg gagccgatca
     1381 ggcagccgga ggtcggaacg atcaggtttg tgaaggcatc accgagctgg aacgcaagga
     1441 cggatgtctg gcgggatacg ccgagaaggt ccgcgagcgg tgccatgatc ggcatggtga
     1501 tcgccgcctg gccggagccg gatacgacga agaaattgaa gaccgactgg aacacataca
     1561 tcagcacggc tgcggctgca ccggagagac cggccagggc ctgtgagatg ccgtacataa
     1621 tggtgttgat gacggtcgga gtcgtcggat cagaaccgcc gagcacctgc atgatgccct
     1681 gtgccatcgc tacaacgagt gccgcaccga tcaggtcctt tgcgccgtct ttgaaggaaa
     1741 cggcaatatc atttactttc atatcgttca gatggaaaat gacaccaatg acacctgcca
     1801 caattcccat gatgaagaac tgggttgcga tttcggccat gtaatagcct tttgccataa
     1861 cgccccagat gacccagaca acggtaacag ccagtgtcac cagaaccagg atatggccga
     1921 tgccgaagga atggccctcg tcgatgcctg ttttttcgtt ctgatcacgg aaatactggt
     1981 cgctttcgta agcaacggag atttccgggg tcttttttac ttttgctgca tagaacatgg
     2041 tcatgccgca gccaagagcg gtgaacacca cccacatcac catacggaaa cctgcgccgg
     2101 agaagacgtc aacgccggcg attccctgtg cgattccgac ggaaaacggg ttcatccagg
     2161 aagatccgaa tccgatctgt gttgccacgt acgtaacgag cacggcaatg acggtgtcgt
     2221 atccgacagc aacgaacagc gggcagagga tcatcgtgaa cggaagggcc tcttctccca
     2281 ttccgaacac ggctccgccg agggaaaaca ggacgaaaag gaccgggatc agcagttttt
     2341 cagcgccttt tgctttacgg atcaggccga tcagaccggc ttcgacagct ccggtgcgga
     2401 tcatgatgcc gaaagcaccg ccgactacca tcagaaatgc gataatttcg atggcagagc
     2461 tgctgataat tccgttatac atgtaattga aaaatccggt tccgccgtcg ccgctgaaca
     2521 gtgcaacgcc ttctgtgacc ggttttccgg cagcatcggt cagatactgg aaacttccgt
     2581 ctttgatgac ggtacgggtt ttttctaccc cgttcatcaa gtatgtgaca tcctccgtct
     2641 cgtaatagcc tttcgggacg aggaaagtca tgacggccgc gataactacc acaaaaaaga
     2701 tgataatgta ggtatccggc acgttaaatc ccctgttgaa tgatttcagg ccttcgcctt
     2761 tctttttgct catattttga gatttcctcc atttgatatt ctattctcgt ttttaacgcg
     2821 catggattag tgtaccatag atcatagagg aaagtcactg aaaaaatcaa aaaa
//
LOCUS       DAOMUF010000226         2824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_10190_length_2824_cov_4.462983, whole genome shotgun sequence.
ACCESSION   DAOMUF010000226 DAOMUF010000000
VERSION     DAOMUF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10190_length_2824_cov_4.462983"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..354)
                     /gene="spoVG"
                     /locus_tag="PLN77_09805"
     CDS             complement(64..354)
                     /gene="spoVG"
                     /locus_tag="PLN77_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HRM44598.1"
                     /translation="MNITDVRVRKVTKEGKMKAVVSITIDDEFVVHDIKVIEGEKGLF
                     IAMPSRKATDGEYRDIAHPINSGTREKIQTIILKQYEQAMAEAEAEEAAAEA"
     gene            complement(383..1498)
                     /gene="glgD"
                     /locus_tag="PLN77_09810"
     CDS             complement(383..1498)
                     /gene="glgD"
                     /locus_tag="PLN77_09810"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HRM44599.1"
                     /translation="MRALGIILAGGNNNKMRELSNKRAVAAMPVAGSFRSIDFALSSM
                     ANSHIQKVAVLTQYNARSLNEHLSSSKWWDFGRKHGGLFVFSPTVTSDNSWWYRGTAD
                     AISQNLSWLKNSHEPYVVIASGDGVYKLDFNKVLEYHIAKRADVTVVCTDSKEADTSR
                     FGVLKMNEDCRIEEFEEKPMVSSSNVISTGIYVIRRRQLIEMIERSAQEDRYDFVKDI
                     LIRYKNLKRIYGYKINTYWSNIATVDSYYRTNMDFLKPEVRDYFFGQYPGIYSKIDDL
                     PPAKYNPGCTVKNSLVSSGCIINGQVENSVLFKKVYVGNNCVIKNSIILNDVYLGDNT
                     HIENCIVESRDTIRANSYHCGEDGIKIVVEKNERYAI"
     gene            complement(1500..2771)
                     /locus_tag="PLN77_09815"
     CDS             complement(1500..2771)
                     /locus_tag="PLN77_09815"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRM44600.1"
                     /translation="MIKKEMIAMLLAGGQGSRLGVLTEKVAKPAVAFGGKYRIIDFPL
                     SNCINSGIDTVGVLTQYQPLRLNTHIGIGIPWDLDKNEGGVTVLPPYEKSTNSEWYTG
                     TANAIYQNMAYMETYNPEYVLILSGDHIYKMDYEVMLDYHKANHADVTIACMPVPIEE
                     ASRFGVMITDGNGRITEFEEKPEHPRSNLASMGIYIFSWKALKESLTALKDQPSCDFG
                     KHILPYCRDNGKRLFAYEFNGYWKDVGTLGSYWEANMELIDIIPEFNLYEEFWKIYTK
                     GDIIRPQYVSGDAVIDRCIIGEGAEIYGEVHNSVIGADVRIEKGVVVRDSIIMRHSTI
                     GEGTQVNKAIIAENVHVGKHVVMGVGEEAPNVLKPNIYGFGLVTVGEKAVIPDGVTIG
                     KNVCISGETSLEDYPDGTLPSGGAIVEKDGE"
BASE COUNT          644 a          721 c          599 g          860 t
ORIGIN      
        1 ccccgggtgg aaaacgtaac cacttccgtt ttccgcccgg gggcattctt tcttcctttt
       61 tgctcaggcc tctgcagcag cttcctctgc ttccgcttct gccatcgcct gttcgtactg
      121 tttcaggata atggtctgaa ttttttctct ggttccggag ttgatcggat gtgcgatatc
      181 gcggtactcc ccgtccgtag ccttacggct cggcattgcg atgaacagtc ctttttctcc
      241 ttcgattacc ttgatatcgt gtacaacaaa ttcgtcatcg attgtgatag acacaaccgc
      301 cttcattttt ccctcttttg tcaccttgcg tactcttacg tctgtaatgt tcataaactt
      361 ttatgcccct ttcaccccat aatcagatgg cgtatctttc gtttttttcg acaacaattt
      421 tgatgccgtc ctccccacag tgataggagt ttgcacggat ggtgtccctg ctctccacaa
      481 tacagttttc aatgtgagtg ttgtctccga gatatacatc atttaagatg atagaatttt
      541 taatcacaca gttgtttcca acatatacct tcttgaacaa aacagagttc tcaacctgtc
      601 cgttgataat acagccgctg gatacaagag aatttttcac tgtgcagcca ggattgtatt
      661 ttgccggcgg aagatcgtcg atcttggagt agatgcccgg atactggccg aagaagtaat
      721 ctcttacctc cggtttcagg aaatccatgt tggttctgta ataggaatct acggtagcaa
      781 tgttgctcca gtaggtatta atcttgtatc cgtaaatccg tttcagattt ttgtaacgga
      841 ttaaaatatc ctttacaaag tcgtaacggt cttcctgtgc gctgcgctca atcatttcga
      901 tcagctggcg acggcggatg acatagatac cggtagagat cacgttggag ctcgaaacca
      961 tcggtttttc ctcgaactcc tcgatgcggc agtcctcatt catcttcagg acgccgaagc
     1021 ggctggtatc tgcttccttg gaatccgtgc ataccacagt aacatccgca cgttttgcaa
     1081 tatgatattc cagtacctta ttgaaatcca gtttgtatac gccatctccc gatgcgatga
     1141 ctacgtacgg ttcatggctg tttttcagcc agctcaggtt ctgactgatc gcgtctgccg
     1201 ttccacggta ccaccagctg ttatctgatg taacggttgg agaaaataca aacagaccgc
     1261 catgttttct tccgaaatcc caccatttgg aagagctcaa atgttcattt aaggatctgg
     1321 cgttgtactg tgtcaggaca gccaccttct gaatatgaga gtttgccatg cttgacagcg
     1381 caaaatcgat gctgcggaag ctgcctgcga ccggcatagc tgcaacggct cttttattgg
     1441 acagctctct cattttgttg ttgttaccgc ctgcaagaat aattcccaat gctctcatat
     1501 tactcaccgt ccttttctac aattgctccg ccggacggaa gtgtgccatc cgggtaatcc
     1561 tcaagggaag tttcgccgga aatgcagaca ttctttccaa tggttacgcc atccggaatg
     1621 actgcctttt ctccaacggt cacaagtccg aatccgtaaa tattcggttt taagacgttc
     1681 ggcgcttcct cgccaacgcc catcacaaca tgtttgccga catgtacgtt ttctgcaata
     1741 atggctttat tgacctgggt tccctcgccg attgtgctgt ggcgcataat aatagaatct
     1801 cttacaacaa cacccttttc aatgcgcaca tccgcaccga tgacggaatt atgtacctct
     1861 ccgtagattt cagcgccctc gccgataata cagcggtcga tgacagcgtc cccggaaaca
     1921 tactgcggac ggatgatatc acctttggta tagatcttcc agaattcctc atataaattg
     1981 aattccggaa taatatcgat cagctccata tttgcttccc agtatgagcc aagcgttccg
     2041 acatctttcc agtagccgtt gaactcatac gcaaacagac gttttccgtt gtcgcggcag
     2101 tacggcagaa tgtgttttcc gaagtcacag ctcggctgat ccttcagggc tgtcagagat
     2161 tcctttaatg ctttccagct gaaaatatag atacccatag aagcgaggtt gcttctcggg
     2221 tgctccggtt tttcttcaaa ttcggtaatt cttccgtttc cgtctgtgat catgacaccg
     2281 aaacggcttg cttcttcgat cggtaccggc atacatgcga tggtcacatc tgcgtggttt
     2341 gctttgtgat agtcaagcat cacctcgtaa tccattttgt aaatgtggtc gccggaaaga
     2401 atcagaacat attctggatt gtatgtttcc atataagcca tgttctggta aattgcattt
     2461 gcagttcctg tataccattc gctgtttgta cttttttcgt atggcggcag aacggttacg
     2521 ccgccttcgt ttttatcgag atcccacgga ataccgattc cgatatgggt attcagccgc
     2581 agcggctgat actgggtcag tacgcctacc gtatcaattc ctgaattgat acaattactg
     2641 agcgggaagt cgatgattcg atactttccg ccaaatgcta ctgccggttt tgccactttc
     2701 tccgtgagca caccgagacg gcttccctgg ccgcctgcca gcagcattgc tatcatctct
     2761 ttcttaatca tgcaatcacc tcttgtcgta tctaaagatt tgcagtaact gtaagaatgt
     2821 acgg
//
LOCUS       DAOMUF010000227         2822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_10200_length_2822_cov_3.474521, whole genome shotgun sequence.
ACCESSION   DAOMUF010000227 DAOMUF010000000
VERSION     DAOMUF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10200_length_2822_cov_3.474521"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLN77_09820"
     CDS             complement(<1..966)
                     /locus_tag="PLN77_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HRM44601.1"
                     /translation="MKFVKERVNVICQELARLSISQKIKITDMQVKNGCYITPAEADA
                     SKEPWTSFDTVNDRWYGPDKHYWFRTTITVPESFDGKNLWMRVHAGLDEWDDGRNPQF
                     LLFANGEVIQGMDINHREVLVRENAKAGEKIQLDLQSYTGTLHSEFRLLADLEEHDAK
                     IEEIYYDLIVPMQGLNRMDEDNKTRLDLETALTNTINLLDLRKPYSKEFYASIEEAEK
                     CIQEEIYEKMGGWDEVVATCIGHTHIDVAWLWTIDQVRQKSCRSFATVLKLMDEYPNY
                     HFMSSQPKLYSFVKERHPEVYEKIRQRVKEGRWEPEGGMWVEADCN"
     gene            complement(1082..2074)
                     /locus_tag="PLN77_09825"
     CDS             complement(1082..2074)
                     /locus_tag="PLN77_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HRM44602.1"
                     /translation="MMSKIIGNNLPDMPWQEKPAGEHMPVWRYDQNPIIGRYEQKRSN
                     SIFNSAVVPFEDGYVGVFRCDSRSISMDIFVGRSKDGIHWEIEDEPIKFEGADEEILT
                     REYRYDPRVCKIDDKYYVTWCNGYHGPTIGVAYTYDFKKFVQLENAFLPFNRNGVLFP
                     RKINGYYMMMSRPSDNGHTPFGDIFVSQSKDMEFWGRHRHMMSPVRGDESAWQCTKIG
                     AGPVPIETDEGWLLIYHGVITTCNGYVYRMGCALLDIDEPWKVKYRTKDYIMAPITQY
                     ECMGDVPNVVFPCATLSDAATGRITIYYGCADTVTGMAFTTVDELLNYVKENSL"
     gene            complement(2289..>2822)
                     /locus_tag="PLN77_09830"
     CDS             complement(2289..>2822)
                     /locus_tag="PLN77_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-beta-hexosaminidase"
                     /protein_id="HRM44603.1"
                     /translation="ELNEQISRLAYGDRSGKFVSYMAKTAECSIFDWWDANVVYEEKV
                     LGHPNNRNALFDARIQDEAKRAAAKETIAALKKELKKTAGALEESCRPMVPVLELTME
                     AIDIWNETGARMCDIELGKEKDETACAALAGRLETWFMKYKASWRSISKEGDLHHIAE
                     IVFWYADILRGRKPYEK"
BASE COUNT          561 a          746 c          630 g          885 t
ORIGIN      
        1 gttgcagtct gcctcaaccc acatgccgcc ttccggctcc cagcgtcctt ctttgactct
       61 ctggcggatt ttctcgtata cttccgggtg tctttccttt acaaagctgt agagcttcgg
      121 ctggcttgac atgaaatgat agttcggata ctcatccatc agttttaata ccgttgcaaa
      181 gcttctgcag gatttctgac gcacctggtc gatggtccac agccatgcta catcgatgtg
      241 tgtgtggccg atgcacgttg ccacaacttc gtcccagccg cccatttttt catagatctc
      301 ttcctggatg catttttctg cctcttcgat ggaagcgtag aactctttac tgtacggctt
      361 gcgcaggtcc agcagattga tcgtgttggt cagtgcggtt tcgagatcca gacgcgtttt
      421 attatcctcg tccatacggt tcaggccctg catcggaacg atgaggtcgt aatagatctc
      481 ctcgattttc gcatcatgct cctcaaggtc tgccagcaga cggaactcgc tgtgcagcgt
      541 tccggtgtag gactgcaggt cgagctggat tttttcaccg gcctttgcgt tttctcttac
      601 aagtacttcg cggtggttga tatccatacc ctggatcact tcgccgtttg caaacagcag
      661 gaactgcggg tttcttccgt catcccattc gtccagtcct gcatggactc tcatccagag
      721 attttttcca tcgaagctct ccggaacggt gatggtggtg cggaaccagt aatgtttgtc
      781 cggtccgtac cagcggtcat tgaccgtatc aaacgatgtc cacggctctt ttgatgcatc
      841 ggcctctgcc ggtgtgatat agcatccgtt tttcacctgc atatcggtga ttttgatttt
      901 ctgtgaaatc gaaagtcttg ccagttcctg gcagatcacg ttcacacgct cttttacaaa
      961 tttcatagtg cctccttatg ctttccatgg ggtcatttta aaaaagaaag tccgccggac
     1021 ctgcaggctt ctttcacccg taagcccggc ggacttgtcg ctttttaagt tatcttataa
     1081 atcaaaggct attctctttc acatagttca gcagctcatc tacggttgtg aatgccatac
     1141 cggttacggt atctgcgcat ccgtagtaga tagtgattct tccggttgct gcatcgctta
     1201 aggttgcgca cgggaatact acgttcggaa catcacccat gcactcgtac tgggtgatcg
     1261 gagccataat atagtctttc gttctgtatt ttactttcca cggctcgtcg atatcaagca
     1321 gtgcgcatcc catacgatat acgtatccgt tgcaggttgt gataacgccg tggtagatca
     1381 gcagccatcc ctcatctgtt tcgatcggaa ccgggccggc accgatcttg gtgcactgcc
     1441 atgcagactc atcgccgcgg accgggctca tcatgtgacg gtgtcttccc cagaattcca
     1501 tatctttact ctggctgaca aagatatcgc cgaacggtgt atggccgttg tcgcttggac
     1561 ggctcatcat catgtagtag ccgttgatct ttctcgggaa cagcacgccg ttacggttga
     1621 atggaaggaa tgcgttctca agctgaacga atttcttgaa gtcataggtg taagcaacac
     1681 cgatggtcgg gccgtgatag ccgttgcacc aggttacata gtatttgtcg tcaattttgc
     1741 agacacgcgg atcataacga tactctctgg tcaggatctc ctcgtcagcg ccctcgaatt
     1801 tgatcggttc atcttcgatc tcccagtgaa taccgtcttt acttcttcca acgaagatgt
     1861 ccatgctgat ggaacggctg tcgcagcgga atacacctac gtatccgtcc tcgaacggta
     1921 cgactgcgct gttgaagata ctgttggaac gtttctgctc gtaacggccg atgatcgggt
     1981 tctgatcata gcgccatacc ggcatgtgct ctcctgctgg tttttcctgc caaggcatat
     2041 ccggcagatt gtttccgatg attttactca tgattgtttc cctctgtgaa aattattttt
     2101 ttctttctct cttgtcacgc catacggaca tttttgatct ttcgatccga tacttctatc
     2161 ttcgtccatt tctgcgcaaa agtcaagtgt ttttgttcac ttttatgtaa attttcgttt
     2221 tctttgttac tttttatcgt tcatttattt tctatttgtg ttcatttttg ttcacaaatg
     2281 gattttttct atttttcgta cggttttctt ccccgcagaa tgtcggcata ccagaataca
     2341 atctctgcaa tgtggtgcag atcgccttct ttgctgatgc ttctccagga cgctttatac
     2401 ttcataaacc aggtttccag ccgtcccgca agtgccgcgc aggctgtctc atccttttct
     2461 tttccaagct caatgtcaca cattctcgcg cccgtctcat tccagatgtc aatcgcttcc
     2521 atcgtgagtt ccagcaccgg aaccattgga cggcaggatt cttccagagc accggctgtc
     2581 tttttcagtt cttttttcag agccgcaatt gtttcctttg cagctgcacg cttcgcctca
     2641 tcctggattc tcgcgtcaaa cagcgcattc cggttgttcg gatgaccgag cactttttcc
     2701 tcgtaaacga cgttggcatc ccaccagtca aagatggagc attctgccgt ttttgccata
     2761 taggaaacga attttccgct ccggtcgccg taggccagac gggaaatctg ttcattcagc
     2821 tc
//
LOCUS       DAOMUF010000228         2792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_10340_length_2792_cov_3.414322, whole genome shotgun sequence.
ACCESSION   DAOMUF010000228 DAOMUF010000000
VERSION     DAOMUF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10340_length_2792_cov_3.414322"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2792
                     /locus_tag="PLN77_09835"
     CDS             <1..>2792
                     /locus_tag="PLN77_09835"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRM44604.1"
                     /translation="RLWEGAMQYEPYKTYKDRFNVYALCTASDKTYSAIDGYDSTFFD
                     VWGKNISVNGSQWKNHIFERCIGPAFIEKVHDAHIPQQADPNVDWDFEKYKYVHDYIS
                     QFVLLVNSANDFGGAFNDLDYGFHYIVSPAYSQRAVETLTHELGHGLLWLGDEYNSGS
                     FMGEASEKTSLNRTGISDPEQVKWRQLLGFRKTYSVPHTDYDTDKIYNSSRECMMRQT
                     WNGFCEVCKLQGNKRMRQLVTEGPDLYVAEPEVTKRTDAYTKLSDFSDATGWGYTKFD
                     ADKKTRLLTGADKITFQPTEMKGQKIELRTIVQNLSDTKLSQVTLQVWVNHADGTIAT
                     ADGQPVAASETFKIPLWTEKGNFRPKGTLEYHGSDFNSGLKNCSLLYTIPSNADLRTG
                     DTVGYAVRDEQGTVLAYEGTLPNKGQDILPAPEPAKSYTVTFCYNDGRENTTKTTGIN
                     GKLGDLPAPAREGYVFDGWYTTGGEKVDLTRVYSSNTVLYARWSEYIAPSPNVKKAPV
                     ILLATSPDTVTEGEQVMLSVSETSGFGVDLSGVTYTADPSLPISGTGEAQTIRLDQAG
                     TYTFTAHYSGDNEKYLAADSNRVTVTVTKKADVSGGTTSGGGSSSGGGSTAGGGSSAG
                     GGSSAGGGSSSGGGAAAGGATAGGGAANGNASATTTPDIKDSDGTTVAIVNGKKGMIT
                     AEVQLSEKAIANAEKSGEAVKLPVEVKAGKNIKAASTVTINLPEGAGKTKVKIPVKNM
                     TAGTVAVLVNADGTEKIVKKSVAAKDGVQLIVDEDTTVKLVDKAKNFKDTKKHWAKDS
                     IDFVSARGLMNGKSSTAFAPEAKITRARLWTILARWEDVDLTGGKKWYSKARAWAKNQ
                     GISDGSRPNAAITRAEAITMLWRAQGKPAAEQETAFKDVSSDEYYAQAVAWAKEKGIA
                     QANSKGRFNPDAACTR"
BASE COUNT          851 a          549 c          781 g          611 t
ORIGIN      
        1 aacggttatg ggaaggcgcg atgcagtatg agccatataa gacgtacaag gatcgcttca
       61 atgtatatgc attatgtacg gcatcggata aaacgtacag tgccattgat ggatatgaca
      121 gtacattttt cgatgtatgg ggaaagaata tatccgtgaa tggaagtcag tggaagaacc
      181 atatttttga acggtgtatc ggaccggcgt ttatcgagaa agtgcatgat gcccatattc
      241 cgcagcaggc agatccgaat gtagattggg attttgaaaa gtataaatat gtacatgact
      301 acatcagcca gttcgtactt ctggtcaatt ctgcaaatga ttttggcggg gcattcaacg
      361 atcttgatta cggattccat tatattgtca gccctgcata cagccagcgt gcagtcgaaa
      421 cgcttacgca tgagcttggt catggtctgt tatggcttgg agacgagtac aacagcggat
      481 cttttatggg agaagcgtct gaaaaaactt cgctaaacag aacggggatc agtgatcctg
      541 aacaggtaaa atggagacag ctgcttggct tccggaagac atacagtgtt cctcatacag
      601 attatgatac ggataagatt tataattcca gccgtgaatg tatgatgcgc cagacctgga
      661 atggtttctg cgaggtatgt aagctgcagg gaaataagcg gatgcgccag ctggtcacag
      721 agggtccgga tctgtatgtg gcagaaccgg aagtgacaaa gcgcacagat gcatatacga
      781 agctttcaga ttttagtgat gcaaccggtt ggggttatac aaaatttgac gcagacaaaa
      841 agacacgcct tttgacaggg gcagataaaa ttactttcca accgacagag atgaaggggc
      901 agaagattga gctgcgtaca atcgtgcaga acctttccga tacaaagctc agtcaggtga
      961 cattacaggt atgggtaaac catgctgacg gaaccattgc tactgctgat ggtcagcctg
     1021 tagcagcgtc agaaacgttc aagattccgc tttggacgga aaagggcaat ttccgcccga
     1081 agggaacgtt ggaatatcat ggaagcgatt ttaattccgg tttgaaaaat tgttccctct
     1141 tatataccat cccgtccaat gcagatctgc gaaccggtga cacggtcgga tatgcagtga
     1201 gggatgagca gggaactgtt cttgcttacg aaggaacgct tccaaacaaa ggtcaggata
     1261 tcttgccggc accggagcct gcaaagagtt atacagttac tttttgttat aatgacggaa
     1321 gagaaaatac aacaaaaacg accggaatca atggaaaact cggtgatctt ccggcaccgg
     1381 cccgtgaagg atatgtgttt gacggatggt atacgacagg tggtgagaaa gttgatttga
     1441 caagagtata tagcagcaat acggtgttgt atgcaagatg gagtgaatat atcgcaccgt
     1501 caccgaatgt taagaaagct ccggtcattc tgctggcgac aagcccggat acagtgaccg
     1561 aaggagaaca ggtcatgtta agtgtcagcg aaacctcagg atttggtgtg gatttaagcg
     1621 gagtaactta cacggcagat ccttcgctgc cgatatccgg cacaggggag gctcagacga
     1681 tccggctcga tcaagctggt acttatacat ttactgctca ttattccggt gataatgaga
     1741 aatatcttgc ggccgacagt aatcgtgtta ccgtaaccgt aacgaaaaaa gcggatgtca
     1801 gcggaggaac aacgtcagga ggtggatcat cgtcaggagg cggttccacg gcaggaggcg
     1861 gatcttcagc aggaggcgga tcttcagcag ggggcggatc ctcgtcagga ggcggtgcag
     1921 cagcaggagg tgccaccgcg ggaggcggcg cagctaacgg aaatgcatct gcaacaacta
     1981 cgccggacat caaggattcg gatggaacga ctgttgccat agtgaacggt aaaaaaggaa
     2041 tgatcacagc agaagtgcag cttagtgaaa aggcgatcgc aaatgctgaa aaatcaggtg
     2101 aagcagttaa acttccggtt gaagtaaagg caggtaaaaa tataaaagct gcatccactg
     2161 tgaccatcaa tctaccggag ggtgcaggga aaacgaaagt gaagattccg gtaaaaaata
     2221 tgaccgcagg tacagtcgca gtgcttgtca atgccgatgg cactgagaaa attgtgaaga
     2281 aatcagtggc tgcaaaagat ggagttcagc tgatcgtgga cgaagacact acagtgaagc
     2341 tcgtagataa ggcgaaaaac tttaaggata caaaaaaaca ttgggcgaag gattccattg
     2401 attttgtaag tgcacgcggg ctgatgaatg gaaagagcag tacggctttt gcaccggaag
     2461 caaaaattac ccgggcacgg ctatggacga ttctcgcacg atgggaagac gtagatctga
     2521 caggtggaaa gaagtggtac agcaaagcac gtgcatgggc taaaaaccag ggaatttcgg
     2581 atggcagcag accaaatgct gcgataacga gagccgaggc gatcacaatg ctatggcgtg
     2641 cgcaaggaaa accggcagca gagcaagaga cagcttttaa agatgtttct tctgatgaat
     2701 actacgcaca ggcagtggcg tgggccaaag agaagggtat cgcacaagcg aatagcaaag
     2761 gacgattcaa tccggatgct gcctgtacaa ga
//
LOCUS       DAOMUF010000229         2779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_10389_length_2779_cov_3.096916, whole genome shotgun sequence.
ACCESSION   DAOMUF010000229 DAOMUF010000000
VERSION     DAOMUF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10389_length_2779_cov_3.096916"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="PLN77_09840"
     CDS             complement(<1..1851)
                     /locus_tag="PLN77_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HRM44605.1"
                     /translation="MKFLPKPKEVKLLTGEHALCYDGRIVLDGRLLGNGDTYAKVLQK
                     GIKKETGMQYDIGYGVPGRKETGAIVLELDETRKSQQYVLQVTEEEIRIQGGDGAGVL
                     YGVQTLCQMMHEYGALLPAVRIEDEPDLPVRGYYLDETRGRVLTLSYLKQVADRMAYY
                     KLNQLQLYVEHTYLFSGLSEMWRDETPLTAEEIRELDAYCAKLHIELVPSIATFGHLY
                     MLLSTKSYGDLCEFPDSWKEPFSFWDRMQHHTVDVSGGRAIELIKAMIEEYMALFATD
                     KFNICADETFDLGKGKSKPLADEKGVHRLYIDYVKELCEFLVAKGKKPMFWGDIICAQ
                     PELIKELPEETVCLTWGYAAEQREHEAKVMAEAGARQYLCPGVGGWNQWMNLVENSYK
                     NIARMCGYARKYHAEGVLNTDWGDCGHINQPDFSLPGMIYGAVFSWGDDTDSFEELNE
                     QISRLAYGDRSGKFVSYMAKTAECSIFDWWDANVVYEEKVLGHPNNRNALFDARIQDE
                     AKRAAAYETIAALKKELKKTAGKLEESCRPMVPVLELTMDAINLWNETGARLCDIELG
                     KEKDEAACAALAGRLESWFMKYKASWRSISKEGDLHHIAEIVFWYADILRG"
     gene            2281..2742
                     /locus_tag="PLN77_09845"
     CDS             2281..2742
                     /locus_tag="PLN77_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM44606.1"
                     /translation="MLNLLSNATKFTQNGGTIIVRIIQKPYYKKGYALYEFHVRDNGI
                     GMSREFQEHLFESLTREETSTVSGIQGTGLGMAINVSHMVQEIAMELLSNAGFSVSLA
                     DDGDVAVETLRTAAPDQFDLVLMDIQMPKLDGYSATRQIRTPDGAIAKVLG"
BASE COUNT          582 a          795 c          602 g          800 t
ORIGIN      
        1 tccccgcaga atatcggcat accagaatac aatttccgca atgtgatgca gatcgccttc
       61 tttgctgatg cttctccagg atgctttgta cttcataaac cagctttcca gccggcccgc
      121 aagtgctgcg caggctgcct catctttttc ttttccaagc tcgatgtcac acagtctcgc
      181 acccgtctcg ttccagagat tgatggcgtc catcgtaagt tccagcaccg gaaccatcgg
      241 acggcaggat tcttccagtt ttcctgctgt ctttttcagt tcttttttca gtgccgcaat
      301 cgtttcataa gcggccgcac gcttcgcctc atcctggatt ctcgcgtcga acagcgcgtt
      361 tcgattattc ggatggccga gcactttctc ctcataaacc acattggcat cccaccagtc
      421 gaaaatggag cattccgcgg tttttgccat ataggaaaca aattttccgc tccggtcgcc
      481 gtaggccaga cgggaaatct gttcattcag ctcctcaaag ctgtcggtat cgtctcccca
      541 ggagaagacg gctccataga tcatgccagg aagggaaaag tccggctgat tgatgtggcc
      601 gcagtcaccc cagtctgtgt tcagaacacc ttccgcatgg tattttctcg catagccgca
      661 catccgtgcg atatttttgt agctgttctc caccaggttc atccactggt tccagccgcc
      721 gaccccagga cagagatact gtcttgcacc agcttccgcc atcacttttg cctcatgctc
      781 tctctgctcg gctgcatagc cccaggtcag gcagacggtt tcctctggca gctctttgat
      841 cagctccggc tgtgcgcaga tgatatcgcc ccagaacatc ggctttttgc cttttgccac
      901 gagaaactcg cagagctctt ttacatagtc gatatacagg cggtgaacgc ctttctcgtc
      961 tgccagcggt ttcgattttc cttttccaag gtcgaacgtt tcgtccgcgc agatgttgaa
     1021 tttatccgtg gcaaaaagcg ccatgtactc ctcgatcatg gccttgatca gctcgatcgc
     1081 tctgccgccg gagacatcca ccgtgtgatg ctgcatccgg tcccagaagg aaaacggctc
     1141 tttccagctg tctgggaatt cacagaggtc gccatagctc tttgtggaga gcagcatgta
     1201 cagatggccg aacgttgcga tcgacggcac cagttcgata tgaagctttg cgcagtacgc
     1261 atccagctcg cggatttcct ctgccgtaag cggcgtctca tcgcgccaca tctcggaaag
     1321 tcccgagaac agatacgtgt gctccacata gagctgcagc tggttcagct tgtagtacgc
     1381 catgcggtct gccacctgtt ttaaatagga aagtgtcagt acacggcctc tcgtctcatc
     1441 gagataatag ccgcgcaccg gcagatccgg ctcatcctcg atccgcacgg caggaagcag
     1501 tgcaccgtat tcgtgcatca tctggcacag cgtctgcaca ccgtagagca cgccggctcc
     1561 gtctccgccc tggatgcgga tttcctcctc tgtcacctgc agcacatact gctggctttt
     1621 ccttgtctca tccagttcca gtacaatcgc gccggtctct tttcttcccg gaacgccgta
     1681 tccgatatcg tactgcattc cggtttcctt tttgattccc ttctgcagca ctttcgcata
     1741 cgtatcccca tttcccagca gtctgccatc cagtacaatt ctgccgtcat agcagagtgc
     1801 atgttccccc gtcaggagtt ttacctcttt tggttttggt aaaaatttca ttttccttct
     1861 cctcttgttt tttgttttct ttgtaaccat agaactttca ctgaacgtta agtaacattt
     1921 tgaaaccact tttctcttct cccccatcct accatatttt ttccacatat tccacaataa
     1981 tttgtaattt tcttcaactt ttttcaaagt ttcttcctgt tctgctcttt tatatgtaaa
     2041 gtaactttcg tttctctttg tttcccattt ccattccgga acaaaatctg tttttcggat
     2101 gcatcgttca ttccattttt tacttttctt ttacacaaac gctggtgaat tttgtctgta
     2161 tcaagtataa tgagatatat gttgtcttat atttttgttt tgtaagcaat ttctttacat
     2221 atttcataac acagggggat ttctattatc ctctgcaaca agctctgcct gaaccagatt
     2281 ctgctgaatc tgctgagcaa tgccacgaaa ttcacacaga acggcggcac catcattgtc
     2341 cgtattattc agaaaccgta ctataaaaaa gggtatgcgc tctacgaatt ccacgtccgc
     2401 gacaacggca tcggcatgag ccgggaattc caggagcacc tctttgaatc gcttacacgc
     2461 gaggagactt ctacggtcag cggcattcag ggtacgggac ttggcatggc gatcaacgtc
     2521 agccatatgg tacaggaaat cgcgatggag ctcctttcga acgccggatt ttccgtcagc
     2581 cttgcagacg acggcgatgt tgccgttgag acgctccgca ccgcagcgcc ggatcagttc
     2641 gatctggtgc tgatggatat tcagatgccg aaactcgacg gatactctgc aacgcgccag
     2701 atccgcacgc cggacggcgc cattgccaaa gtgcttggct gattcccctt tcttatctgc
     2761 cggaaccgtg aattttctc
//
LOCUS       DAOMUF010000230         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_10585_length_2745_cov_3.613383, whole genome shotgun sequence.
ACCESSION   DAOMUF010000230 DAOMUF010000000
VERSION     DAOMUF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10585_length_2745_cov_3.613383"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1707
                     /locus_tag="PLN77_09850"
     CDS             109..1707
                     /locus_tag="PLN77_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM44607.1"
                     /translation="MGRFVNPDNSAFQVALNSEIYVDKTGLLSYTNKVLDTIQGYICN
                     SRPRRFGKSFAANMLAAYYSKGCDSRKMFSGLAISQTADFEKHLNQYDVIHLDIQWFL
                     SNVSDPEQIVAYITECTLAELRTIYADQLPPEVSTVPDALSRIKEATGQKFVIIIDEW
                     DVLIRDEASNQKIQDDYINFLRGMFKGTEPTKYIQLAFLTGILPIKKLKTQSALNNFH
                     QYSMLNPGPLASYIGFTEDEVSALCQKYGQNFEEVRRWYDGYLLGNYHVYNPNAVTSL
                     LLDGTFQSYWSETSTYDSMVPFINMDFDGLKTAILEMLSGAAVPVLVTSFKNDMVSFV
                     NKDDVLTLLIHLGYLAYNQQTQMAYIPNEEIRREFLTAVTSNRWNELLTFQQESAELL
                     DATLAMDENAVAAGIGKIHEEYTSVIQYHNENSLSCVLTIAYLSSIQYYFKPIRELPT
                     GRGFSDFVFLPKPEYRYDFPALVVELKWNKNAKTALDQIKRQHYPASIASYTGDILLV
                     GVNYDKKTKVHECRIEKYLKSGEK"
     gene            1707..>2745
                     /locus_tag="PLN77_09855"
     CDS             1707..>2745
                     /locus_tag="PLN77_09855"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRM44608.1"
                     /translation="MKVVIAIDSFKGSLSSLQAGTAAATGIYRVDPKVETVVRPLADG
                     GEGTVQALTEGLGGRLVSVSVTGPAGKQVICPYGIIDRTQTAIIEMSGAAGITQVHGD
                     EKNPLYTTTYGVGEVICDAIRNGCHRFIIGIGGSATNDGGIGMLQALGFGLLDAEGKQ
                     VPFGAVGLESLTAITTDHVLPELSECTFRIACDVTNPLCGSNGCSAVFGPQKGADAVM
                     IKRMDTSLASYAALCRKTFPNVDAEFPGTGAAGGLGFAFQTFLSASLEPGIQIVLDET
                     RLRDDVRDADLVITGEGQLDGQTVMGKAPIGVAKLSKEYGKPVLAFSGCVTKDASACN
                     AAGIDAFFPILR"
BASE COUNT          698 a          619 c          706 g          722 t
ORIGIN      
        1 tcggttcttt tccgcgattt tacgggattt tcgggggtgt gcgttgctat ttggatgggg
       61 gaatggtaaa atcggggtat aatcaaaaaa cggaagggaa gtgaaatcat gggaagattt
      121 gtgaatccgg acaacagtgc atttcaggtt gctctcaatt ctgaaatcta tgtggacaaa
      181 acaggacttt tatcgtatac caacaaagtt ctggacacca tacagggcta tatctgcaac
      241 agccgcccgc ggcggtttgg aaaatcattt gctgcaaata tgctggcggc ctattacagc
      301 aagggctgtg attccagaaa gatgttttct ggccttgcga tcagccagac ggcagacttt
      361 gagaagcatc tgaaccagta cgatgtgatt catctggaca tccagtggtt tctttcgaat
      421 gtcagcgatc cggaacagat tgtggcctac attacagaat gtacactggc cgagctgcgt
      481 accatctatg cagatcagct tccgccggaa gtttccaccg ttcccgacgc tctgtcccgg
      541 atcaaagagg cgaccggaca gaaatttgtg attattattg atgaatggga tgtcctgatt
      601 cgtgatgagg cctccaacca gaaaattcag gatgattaca ttaattttct tcgtggaatg
      661 ttcaaaggga cggaaccgac gaaatatatc cagcttgcgt ttctcacggg tattcttccg
      721 atcaaaaaat tgaaaacgca gtctgcctta aataattttc atcagtattc catgctgaat
      781 ccaggcccgc tggcttccta tattggattc acagaagatg aagtttccgc tctctgtcag
      841 aaatacggac aaaattttga ggaagtcaga cgatggtacg acggctatct gcttggaaat
      901 taccatgtat acaatccaaa cgcggtgaca agtctgcttc tggatgggac attccaaagc
      961 tattggtcgg agacgagcac atacgactcg atggttcctt ttatcaatat ggattttgac
     1021 ggattgaaaa cagcaattct ggagatgctt tccggcgccg cagttcccgt actggttact
     1081 tcctttaaaa atgatatggt cagctttgtg aataaagatg atgttctgac gctgctgatt
     1141 catctagggt atcttgccta taatcagcag acgcagatgg catacattcc aaatgaagaa
     1201 atccgccggg aatttttgac ggcggttact tccaaccgct ggaatgaact tcttaccttt
     1261 cagcaggaat ctgcagagct tctggatgct acacttgcta tggatgaaaa tgcggttgct
     1321 gcaggaattg gaaagatcca tgaggaatat acttctgtga ttcagtatca taatgaaaat
     1381 tcactcagtt gtgtgctcac gattgcttat ttaagttcca tacaatatta cttcaaaccc
     1441 attcgtgaac ttccgaccgg gcgcggtttt tccgattttg tctttctgcc aaagccggaa
     1501 taccgatacg attttccggc gcttgtagta gaactgaaat ggaacaagaa tgcgaaaacg
     1561 gcactggatc agattaaaag acagcattac ccggcttcca ttgcatctta tacaggggat
     1621 attctgctgg ttggtgttaa ttatgataag aagacgaagg tgcatgagtg tcggattgaa
     1681 aaatatttga aatcaggaga aaaatgatga aagttgtgat tgcaattgat tcttttaaag
     1741 gaagcctttc ctctctgcag gcaggaacag cggccgccac cgggatttat cgtgtcgatc
     1801 cgaaggtgga gacggttgtg cgcccgcttg ccgatggcgg tgaaggtacc gttcaggcac
     1861 tgaccgaagg tcttggcgga cggcttgtct ccgtttcggt gaccggtcct gccggaaagc
     1921 aggttatctg cccgtatggc atcatcgacc ggacgcagac cgccattatc gagatgtcgg
     1981 gggctgccgg tattacgcag gtacacggcg atgagaaaaa tccgctgtac accacaacgt
     2041 acggtgtcgg tgaggttatc tgcgatgcca tccgcaatgg ctgccaccgc tttatcattg
     2101 gaatcggcgg aagtgccaca aatgacggcg gtattggtat gctacaggca ctcggtttcg
     2161 gactgctcga tgcagaagga aaacaggttc cttttggtgc cgtcgggctg gaatctctta
     2221 ccgcgattac gacagatcac gttctgccgg aactttcgga atgcaccttc cgtattgcct
     2281 gcgatgtaac gaatccgctc tgcggttcca acggctgcag cgccgttttc ggaccgcaga
     2341 agggcgcgga tgctgtcatg atcaaacgta tggacacatc gcttgcctcc tacgccgcac
     2401 tctgccggaa aacattcccg aacgtcgacg cggaatttcc cggaacgggt gctgcgggcg
     2461 gacttggatt tgcttttcag acgtttcttt ctgcttcact ggaacccggc attcagatcg
     2521 tactggacga aacccgactt cgtgatgatg tgagagacgc tgatcttgtc atcaccggtg
     2581 agggacagct cgatgggcaa accgtgatgg gaaaagcgcc gattggagtt gcaaaactgt
     2641 caaaagaata cggcaaacct gtccttgcgt tctcaggctg tgttacaaaa gatgcatctg
     2701 catgcaacgc cgccggaatt gatgcctttt tcccgattct gcgga
//
LOCUS       DAOMUF010000231         2709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_10783_length_2709_cov_3.410324, whole genome shotgun sequence.
ACCESSION   DAOMUF010000231 DAOMUF010000000
VERSION     DAOMUF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10783_length_2709_cov_3.410324"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLN77_09860"
     CDS             <1..702
                     /locus_tag="PLN77_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRM44609.1"
                     /translation="ERTKNGTMPENPALVETIVTTDMAKAIAKAYNVALIEVLTGFKY
                     IGEQIKFFEQSGAHNYVFGLEESYGCLAGTYARDKDACVAVMMLCEVAAYYKQQGKTL
                     WDAMVDMYEEYGYYKEGLATMTLKGIDGAKEIQTMMTNFRENPPKELGGFQVLAVRDY
                     KADVRQDLVSGEKSATGLPSSNVLYYELENNAWCCVRPSGTEPKIKFYFGVKGTSLED
                     AAEKLEKLKNAMVTA"
     gene            810..>2709
                     /locus_tag="PLN77_09865"
     CDS             810..>2709
                     /locus_tag="PLN77_09865"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRM44610.1"
                     /translation="MAILYYDEKKLFQLNTENTTYVIGLSPEGYVGHVYYGPLLHGEP
                     DLYPLRMDEPPFTPSVNKREKSSFLDRFPMEYPTGGVGDYRESCLNIRNAQGRMGCEI
                     FFDSYEIFKGKRPLTGLPASFGTEDEVETLEITCKDPVLDLVVVLSYSVFTKENVITR
                     SVRVKNEGSEALKVEKIYSACLDMDNEDFEMLSLHGSWGRERHIQQGALRYGKQLVSS
                     GKGESSHQEHPFVAMVTPGTDQERGEVYAMHFVYSGNFIGQVERCQFDTVRMVMGINQ
                     EEFCWNLKAGDEFQAPEVVMVYSAEGLGKMTRSYHAFYRRHMIRSPYNHKKRPILINN
                     WEATYFDFDTDKLLDIAREAKKDGIEMLVMDDGWFGKRNKDDSSLGDWVVNEEKIKGG
                     LKNLVDKVNEIGLEFGIWFEPEMISPDSDLYREHPEWAIQIPGREATEIRCQYVLDLS
                     RPEVQDYAYECVAKILRSANIKYVKWDMNRQLSDLGSTYLDKDSQQELFHRYVLGMYA
                     MQERLVQEFPDLLLENCSGGGARFDPGMLYYSPQIWCSDDTDAIERLEIQEGTALIYP
                     LCSMGAHVSVCPNHTVGRVTPFTTRGHVALAGTFGYELDITKLPEEERKLIPEQTAMY
                     HKYHELIRE"
BASE COUNT          753 a          626 c          728 g          602 t
ORIGIN      
        1 gagcgcacca agaacggaac catgccggag aacccggcac tcgttgagac gattgttacg
       61 accgatatgg caaaagcaat cgcaaaagct tataacgtgg cactgatcga ggttctgacc
      121 ggattcaaat acatcggcga gcagatcaaa ttttttgagc agagcggtgc acacaattat
      181 gtatttggtc tggaagagag ctatggctgc ctggccggga catatgcaag agataaagat
      241 gcctgcgtgg cagttatgat gctctgcgag gttgctgcat actacaaaca gcagggcaaa
      301 acactgtggg atgccatggt ggatatgtac gaggagtacg gctactacaa agagggtctt
      361 gcaacgatga ccttaaaagg tatcgacggt gcaaaagaga tccagaccat gatgaccaac
      421 ttccgtgaga acccgccaaa agagctgggc ggtttccagg ttcttgccgt acgcgattac
      481 aaagcagatg tacgccagga tctcgtaagc ggagagaaga gcgcgaccgg acttccgtcc
      541 tccaacgttc tttactatga gctggaaaac aacgcatggt gctgtgtgcg cccatccggt
      601 actgagccga agatcaaatt ctatttcggc gtaaaaggca catcccttga agatgcagcc
      661 gagaaactgg aaaaattaaa aaatgcaatg gtaactgctt aaacgctgtg tgatatagaa
      721 acaaaaaaag gcctcagccc ggcgggtgag gtctttttgt acaaaaatcg gcccggcgga
      781 tcaagcttac ctgaaataag gagaagaaaa tggcgatttt atattatgat gagaaaaagc
      841 tttttcagtt aaatacagag aatacgacgt atgtgatcgg cctgagcccg gaaggctacg
      901 tcggacatgt ttactacgga ccccttctgc acggagagcc ggatctgtat ccgctccgca
      961 tggatgagcc gccgttcacc ccgtccgtca ataagagaga gaaatcttcg ttcctcgacc
     1021 gttttccaat ggaatacccg accggaggag tcggcgatta ccgtgagagc tgtctgaata
     1081 taagaaatgc acagggccgt atgggatgtg agattttctt tgattcctat gaaattttca
     1141 aaggaaaacg cccgctgacc ggtcttccgg cttcttttgg aaccgaggac gaagtagaaa
     1201 ctctggaaat cacctgcaaa gatcctgttc ttgatcttgt tgttgtcctt tcctactctg
     1261 tttttacaaa agaaaacgtt atcacccgaa gtgtccgcgt gaaaaatgaa ggcagtgaag
     1321 cgctgaaggt agagaaaatc tacagtgcct gcctcgatat ggacaacgaa gactttgaga
     1381 tgctttctct tcatggatcc tggggaagag aacgccacat ccagcagggt gcgctccgtt
     1441 acggaaaaca gctcgtttcc tccggaaaag gagagtccag ccatcaggag catccgttcg
     1501 tggcaatggt aacaccggga acggatcagg agcgcggcga ggtttacgca atgcacttcg
     1561 tatattccgg caactttatc ggacaggtgg aaagatgcca gtttgatacc gttcgtatgg
     1621 tcatgggtat caaccaggag gagttctgct ggaatttaaa agccggcgat gaattccagg
     1681 cgccggaggt tgttatggtc tactctgccg agggacttgg caaaatgacg cgttcctacc
     1741 atgcatttta tcgcagacat atgatccgca gcccgtacaa ccacaaaaaa cgcccgattc
     1801 tgattaacaa ctgggaagca acttactttg attttgatac cgataaactc ctcgatatcg
     1861 caagagaggc aaaaaaagac ggcatcgaga tgcttgttat ggatgacggc tggttcggaa
     1921 aacgaaataa agacgattct tccctcggtg actgggttgt caacgaagag aagatcaaag
     1981 gcggcctgaa aaatctggtt gacaaagtga atgaaatcgg tcttgaattc ggtatctggt
     2041 tcgagccgga gatgatctcc ccggactctg atctctacag agagcacccg gaatgggcga
     2101 ttcagattcc gggaagagaa gcgactgaga tccgctgcca gtacgtgctg gatctctccc
     2161 gcccggaagt gcaggattat gcgtatgaat gtgtggcaaa aatcctgcgc agtgcaaaca
     2221 tcaaatacgt gaaatgggat atgaaccgtc agttaagcga cctgggaagc acctacctgg
     2281 acaaagattc ccagcaggag ctgttccacc gctacgtgct cggtatgtat gcgatgcagg
     2341 agcgtctggt acaggagttc ccggatctgc ttctggagaa ctgctccggc ggcggtgcac
     2401 gtttcgatcc tggaatgctt tactacagtc cgcagatctg gtgctccgac gacaccgatg
     2461 cgattgagcg tctggaaatc caggagggaa cagccctcat ttacccgctc tgctcaatgg
     2521 gtgcacacgt cagcgtatgc ccgaaccaca cggttggccg tgtaactccg tttacaacga
     2581 gaggccatgt ggcactggcc ggaacgttcg gctatgagct tgatatcaca aaactgccgg
     2641 aagaagagag aaagctgatt ccggagcaga ccgcgatgta tcacaaatat catgaactga
     2701 tccgcgagg
//
LOCUS       DAOMUF010000232         2709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_10784_length_2709_cov_2.686511, whole genome shotgun sequence.
ACCESSION   DAOMUF010000232 DAOMUF010000000
VERSION     DAOMUF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10784_length_2709_cov_2.686511"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PLN77_09870"
     CDS             complement(<1..1023)
                     /locus_tag="PLN77_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRM44611.1"
                     /translation="MLKITRKNTLLLGTLILTASGIICRILGFLYRIFLSRRIGAEAF
                     GIYQLATPLYALMLSLGAGGMQTVLSRFVASYLAKKDSRRAKICLAAGCGIVVILSFS
                     ASIFLFFSADFIGTRLLFEPRTILLLRILSLGILPSGIHNCLSAWYLGQNQTALPSFC
                     QLLEQFVRMGASYLAWLFCLSTSLPSAAVAAFGTAAGELFSCLFLMACLSVSHSRSPQ
                     KHRPISVFFKEKTLWYDTFRELAVLNLPLTLNRILLNVLHSIESALLPGCLVTSGLSR
                     KAALSQYGILTGMALPMIFFPSAITHAVSVMLLPGVAKQQADGTPEQISDTIRYTISF
                     CVLLGTS"
     gene            1182..1637
                     /locus_tag="PLN77_09875"
     CDS             1182..1637
                     /locus_tag="PLN77_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRM44612.1"
                     /translation="MKLSTKGRYGLRAMIDLASHEEEGAVSIASISERQNISESYLEQ
                     LVRPLRKAGFIESVRGAGGGYVLAKPADSISVGDVLRVLEGGLEAVTCSVNEGLGGCD
                     AADFCVTRFVWQRINEGITNAVDSISLGELVQQSRQLEKNRNSDRNDKE"
     gene            1644..>2709
                     /gene="nifS"
                     /locus_tag="PLN77_09880"
     CDS             1644..>2709
                     /gene="nifS"
                     /locus_tag="PLN77_09880"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HRM44613.1"
                     /translation="MIYLDNAATTKTAPEVVEAMLPYFSEYYGNPSSIYAIAGKSKEA
                     ITKGREQIANVLGAKPEEIYFTAGGSESDNWALKATFEAYKNKGNHIITTKIEHHAIL
                     HTCEYLEKERGAKITYIGVDENGVVNLDELEAAITPETILISVMAANNEIGTIQPIKE
                     IGRIAKEHNILFHTDAVQAFGQIPINVDECNIDMLSSSGHKINGPKGIGFLYIRKGVK
                     IRSFVHGGAQERKRRAGTENVPGIVGYGVAAERAARTMAERTAKEIEVRDYLINRILT
                     EIPNCRLNGDPVKRLPNNCNISFEFIEGESLLIMLDMKGICASSGSACTSGSLDPSHV
                     LLAIGLPHEKAHGSLRLTLSE"
BASE COUNT          800 a          605 c          768 g          536 t
ORIGIN      
        1 ggaagtgcca agcagcacac agaaagaaat tgtataacgg atcgtgtctg atatttgctc
       61 cggcgtaccg tcggcctgct gttttgctac gccgggcaga agcatgacgg acaccgcgtg
      121 ggtgatggca gatggaaaaa agatcatcgg aagtgccata ccggtgagaa tgccatactg
      181 cgagagcgct gctttccggg aaagaccgga ggtgaccaga cagccgggga gcagagcact
      241 ttcaatgctg tggaggacat tcagaagaat acggttcagc gtaagcggaa gattcaaaac
      301 ggcaagctct cggaaggtat cgtaccagag ggtcttttct ttgaaaaaaa cagaaatcgg
      361 gcggtgtttc tgaggggacc tgctgtgcga gacggacagg caggccatca gaaagagaca
      421 cgaaaagagt tcgccggcgg cagtaccgaa tgcagcgaca gcggcagagg ggaggcttgt
      481 ggaaagacag aaaagccagg caaggtagga agcgcccatg cgcacgaact gttccaaaag
      541 ctggcaaaaa gaaggcagtg ccgtctgatt ctggccaaga taccatgccg agagacagtt
      601 gtggatcccg gaggggagaa tgccgagtga taaaatccgg aggagcagaa tcgtccgcgg
      661 ctcaaaaaga agccgtgttc cgataaaatc cgcgctgaaa aagagaaaga tggaggcgga
      721 gaaggaaagt atcactacaa tgccgcatcc ggccgccaga cagatctttg cccgtctgga
      781 atcctttttg gcaaggtacg atgcgacaaa gcgcgagagc accgtctgca tgccgcctgc
      841 gccaagggag agcatcagtg cgtagagtgg tgtggcgagc tggtagatac caaaggcttc
      901 cgcaccgatc cggcgcgaca ggaatattct atagagaaat ccaagaattc tgcagatgat
      961 cccggacgcg gtcaggatca gtgtgccaag aagaagcgtg ttctttcttg tgattttcag
     1021 catgggatag actcctttca cggcattgcg cagatagtct aaaatatgcg aaaaaactcc
     1081 ttgacagaac cccgtgatag gtgctattct gagtgagaaa taaatccgat taaaactgtc
     1141 ggaattggaa aacgacagca caggaaaaag gagaaccagg aatgaaatta tcgaccaaag
     1201 gaagatatgg tctgcgtgcc atgatcgatc tggcgagtca cgaagaagag ggtgcagtgt
     1261 ccattgccag catttcagaa cgccagaata tctcagagag ctatctggag cagctggtgc
     1321 gtccgcttcg aaaagccgga ttcattgaga gtgtgcgggg agcaggcggt ggatatgtac
     1381 ttgcaaaacc ggcagattcc atttcggtag gagatgttct ccgtgtgctg gagggcggac
     1441 tcgaggcagt cacgtgctcg gtcaatgaag gcctgggcgg ctgcgatgcg gccgatttct
     1501 gcgtcacgcg ttttgtgtgg cagaggatca atgagggaat tacgaacgct gtggattcca
     1561 tcagcctcgg agagctggtt cagcagagcc ggcaattaga gaaaaacaga aacagcgata
     1621 gaaatgacaa ggagtgaaca acaatgattt atctggacaa tgcggcaaca acaaaaacag
     1681 ccccggaagt tgtcgaggct atgcttcctt atttcagcga atactacgga aatccatcca
     1741 gtatttatgc aattgcagga aaaagcaaag aagctattac gaagggaaga gaacagatcg
     1801 caaacgtgct cggcgcaaaa ccggaggaaa tttatttcac agccggcggc agcgagtccg
     1861 ataactgggc gttaaaagct acctttgaag catataaaaa taaaggaaac cacatcatca
     1921 ccacaaaaat cgagcatcat gcgattcttc atacctgtga gtatctggaa aaagagcgcg
     1981 gtgcgaaaat cacctatatc ggcgtcgatg agaacggcgt tgtcaatctg gatgaactcg
     2041 aagcagccat cacacctgag accattctga tttccgttat ggcggcaaac aacgaaatcg
     2101 gaacgattca gccgatcaag gaaatcggaa gaattgcaaa agaacacaat attttattcc
     2161 acacagatgc cgtacaggca tttggccaga tcccgatcaa tgtggatgag tgcaatattg
     2221 atatgctcag ttccagcggt cataagatta acggaccgaa aggaatcggc ttcctttaca
     2281 tccgaaaggg tgtgaaaatc cgctccttcg tacacggcgg tgcgcaggaa agaaaacgcc
     2341 gtgccggaac agaaaatgta ccgggcatcg taggatatgg agttgcggca gagcgtgcgg
     2401 caagaactat ggcggagcgt accgcaaaag aaatcgaagt acgtgattat ctgatcaacc
     2461 gtattttaac cgagattccg aactgccgtc tgaacggcga tccggtaaaa cgtctgccga
     2521 ataactgcaa catcagcttt gaattcatcg agggagagtc ccttctgatc atgctggata
     2581 tgaaaggcat ctgtgcgtcc agcggttccg catgtacttc cggatccctg gatccgtccc
     2641 acgtactgct ggccatcgga ctgccacacg agaaagcaca cggttcctta cgtctgactt
     2701 taagtgagg
//
LOCUS       DAOMUF010000233         2704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_10813_length_2704_cov_1.826727, whole genome shotgun sequence.
ACCESSION   DAOMUF010000233 DAOMUF010000000
VERSION     DAOMUF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10813_length_2704_cov_1.826727"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLN77_09885"
     CDS             complement(<1..704)
                     /locus_tag="PLN77_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRM44614.1"
                     /translation="MEKSTVYFTDFRCPVGTSQLDKLKKLCIAAGIKDIDMEGKFVAI
                     KMHFGELGNMAFLRPNYAKVVADLCKEQGGLPFLTDCNTLYPGSRKNALEHLDCANLN
                     GFNTITTGCQIIIGDGLRGTDEVEVPVVNGEYCKTALIGHAIMDADIFISLSHFKGHE
                     ATGFGGALKNIGMGCGSRAGKMQQHASGKPAINTDVCRGCHRCAKECGSDAISYVDKK
                     AVIDYDKCKGCGRCIGA"
     gene            885..1544
                     /locus_tag="PLN77_09890"
     CDS             885..1544
                     /locus_tag="PLN77_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM44615.1"
                     /translation="MIKAVLFDMDGILFDTEIVMKEGWLKAARELNFTLTEEHLSQMR
                     GSALPRSRKLFEEWFHGKVTYDEGRAIRSAYLNDYIQRNGVPEKPGLHEFLSWLKEHS
                     IKVAVATSTTRRVAEGYWKQSGIDQYIDASVCGDEVVNSKPDPEIFLKAASLLQVPIA
                     ECMVAEDSINGLKAARASGAVSCMIPDLTPYTEELAPICDHVSPSLLDCRKIILDSQE
                     K"
     assembly_gap    1580..1589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1736..>2704)
                     /locus_tag="PLN77_09895"
     CDS             complement(1736..>2704)
                     /locus_tag="PLN77_09895"
                     /inference="COORDINATES: protein motif:HMM:NF037025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HRM44616.1"
                     /translation="DPSENGAFPYKKETVYEVKTSTGNGIITFAKQFVGRPYVWGGNS
                     LTDGIDCSHFVWQILTRCGAYDGEYTTSGGWRSLGTEVASLDEARAGDVICYNGHVAL
                     YDGEGKIVEALNENAGITCDRPVDCDTILTIRRFAADDELGGTNAEKIWNYFLMHGFT
                     KEGAAGIMGNIANEASTDLNPTLLEYGSTSRTSLSGEQYTNLVEVIRSSRFGLYSGGR
                     YGYGLCGFTDPTIKEYLCRYTIDLGKSLGSLSGQLDSLMAYLSDYNPNLLDRLKNAED
                     VDTAATAFMREYEKCANQSTQQKLRTTAAEQIYNVMESYDSPVDVE"
BASE COUNT          640 a          664 c          629 g          761 t
ORIGIN      
        1 gcaccgatgc agcggccgca tcctttgcat ttgtcatagt caatgactgc ttttttatcc
       61 acataggaaa ttgcatcgct tccgcattct tttgcacagc ggtggcagcc acgacagaca
      121 tccgtattga tcgccggttt tccactggca tgctgctgca tctttccggc gcggcttccg
      181 catcccatac cgatattttt cagtgcaccg ccaaagcccg ttgcctcatg gcctttgaaa
      241 tggctcaagc tgataaagat atctgcgtcc atgatcgcat ggccaatcaa tgctgttttg
      301 cagtattctc cgttgacaac cggaacttct acttcatcgg ttccgcgcag accgtctccg
      361 atgataatct gacaaccggt cgtaatggta ttaaatccat tcagatttgc gcagtcaaga
      421 tgttccagcg cattctttct gctgcctgga tacagcgtat tgcagtctgt cagaaacgga
      481 agaccgccct gctctttgca gagatcggca accactttcg cataattcgg gcgaagaaac
      541 gccatgtttc ctagttcgcc gaaatgcatt ttgattgcga cgaatttccc ttccatatca
      601 atatctttga tgccggcagc gatacagagt tttttcagct tgtcaagctg actcgttcca
      661 accggacatc gaaagtcggt aaaataaacc gttgactttt ccattttgtc attcctcctg
      721 atttgtcatt ttttgacagc atatcacttc aagctaggtt gaagtcaagt ataatttgtg
      781 gtcttctgtt tgtctcatgg acttgtcttt tttcgggatt tttcgtatac tgaacatagt
      841 atttaagcaa ctgaaaatac agaagtgctt tggaggtcat gtttatgata aaggcagtat
      901 tattcgatat ggatggtatc ctgtttgata ccgagattgt aatgaaagag ggctggctca
      961 aagctgcccg ggagctgaat tttacgctca cggaggaaca tctctcacag atgcgcggca
     1021 gcgcgcttcc acggagcaga aagctgtttg aggagtggtt tcacggaaag gtgacatacg
     1081 atgagggacg cgcgatccgc tcggcgtatc tgaatgatta tattcagcga aatggtgtac
     1141 cggaaaagcc gggacttcac gagtttcttt cctggctgaa ggaacattcg attaaagttg
     1201 cggttgccac ttccacaaca cggcgcgttg cggaaggata ctggaaacag tccggcattg
     1261 accagtatat cgatgcttcg gtctgtggag atgaggttgt aaacagcaaa ccggacccgg
     1321 agattttctt aaaggccgct tctcttctgc aggttccgat tgcagagtgc atggtagccg
     1381 aggacagcat caacggtctc aaagctgccc gcgcctccgg cgctgtctcc tgcatgatcc
     1441 cggatctgac accgtatacg gaagaactcg cgccgatctg tgatcatgtg agtccgtcgc
     1501 ttctggactg caggaaaatt attctggatt cacaggaaaa ataatcaatc gtaaagaaag
     1561 tgcctgtcag tgtttttcan nnnnnnnnng ctgcagactc cggaagcgga caaactgaaa
     1621 agctggaagt aaaaaatcat acataaattc aattatttta ctgtaagaaa gtcctccaca
     1681 cagaatcttt ctgctgcgga ggactttcct taacacgttt tcatcatctg ttcgtttatt
     1741 ctacatctac cggcgaatca tacgattcca taacattgta gatctgttct gctgctgttg
     1801 tacgcagttt ctgctgtgtg ctctggtttg cgcatttttc gtattcacgc atgaaggcag
     1861 ttgctgcggt gtcaacatct tcggcatttt tcagtctgtc caacagattc ggattgtagt
     1921 cggaaaggta ggccatcaga ctgtcgagct ggccggacag gcttccaagg ctctttccaa
     1981 gatcaatggt atatcggcac agatactctt tgatcgttgg atctgtaaaa ccacagagac
     2041 catatccata tcttccgcca ctgtaaaggc caaaacggga acttcggatc acttcaacaa
     2101 ggttcgtgta ctgctctccg ctcaggcttg tgcggctggt agagccatac tcaagcagtg
     2161 tcggattgag gtctgtggat gcctcgtttg caatatttcc cataataccg gcggctcctt
     2221 cttttgtaaa tccgtgcatc aggaagtaat tccagatctt ttctgcgttc gttccgccga
     2281 gttcatcgtc ggctgcaaaa cgacggatcg tcagaatcgt gtcacagtcc accgggcggt
     2341 cgcaggtgat accggcgttt tcgttcagcg cttctacgat ttttccttct ccgtcataca
     2401 gcgccacatg gccattatag caaataacat cgcctgcgcg tgcctcatcc aggcttgcaa
     2461 cctccgttcc aaggctgcgc catccgccgg aggtcgtata ttcaccgtcg tacgcaccgc
     2521 aacgggtcag aatctgccat acaaaatgag agcagtcaat accgtccgtc agggagtttc
     2581 caccccagac gtatggtctg ccaacgaact gttttgcaaa tgtgataata ccgtttccgg
     2641 ttgaggtttt tacttcataa accgtttctt ttttataggg aaaggctcca ttttcagacg
     2701 gatc
//
LOCUS       DAOMUF010000234         2680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_10926_length_2680_cov_2.809143, whole genome shotgun sequence.
ACCESSION   DAOMUF010000234 DAOMUF010000000
VERSION     DAOMUF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10926_length_2680_cov_2.809143"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLN77_09900"
     CDS             complement(<1..755)
                     /locus_tag="PLN77_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRM44617.1"
                     /translation="MASLKKRAKEFETPYPVTKAITKGDAVTKLSMLVMGLGNLAHKQ
                     IVKGILFLAVEIAYLLFMIEGGINNLYHLITLGGRAQEEVWNEAKGIYEYTGGDMTIL
                     FLLYGVATIFITVLFFMIWRVNMKSAYEVECRAKEGKHINTIKEDLEALVDKRLHWTL
                     LTLPILGIVIFTILPLVFMICMAFTSYSKVGDHLTIFHWVGLKNFSTVLGMGNSIGKT
                     FWSVLGWTLIWAVLATFLNYIFGMILAIVINRKT"
     gene            complement(857..2170)
                     /locus_tag="PLN77_09905"
     CDS             complement(857..2170)
                     /locus_tag="PLN77_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM44618.1"
                     /translation="MKKKVVSIMMTAALAVGMLAGCGNSDSGSSNAASGNDSKSTSEA
                     GSTSASASNAAEEEEITTTLTVWSPSEDQASDSGEWLQTMCNQFAEEHPSWHITFDYG
                     VCAESDAKDTVTQDVEGAADVYMFANDNLTSLIAANGISKLGGQTVEEVKENNSQAIV
                     DSVSVDGDIYGVPFTTNTWFMYYDKSAFTEDEVKNLDTMIAKDKISFPITNSWYIASF
                     YVANGCTLFGEDGTDEAAGVDFEGDKAKAVTDYLVDLVNNPNFVSDADGSGLSGLRDG
                     SVKAMFSGSWDASAVKEALGDNYGVVQLPTITINGEEKQMKSFSGSKAIGVNPNCEYP
                     QVAVALARYLGSADAQKAHYEMRNVVPCNEELLSQITDDALVTAQNDTFNNTSIIQPF
                     VKNMSNYWTPAENFGKSLVNGEVTHDNAAEMTEKLNTSMNTSAVE"
     gene            complement(2448..>2680)
                     /locus_tag="PLN77_09910"
     CDS             complement(2448..>2680)
                     /locus_tag="PLN77_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44619.1"
                     /translation="RTKTTETYRYTLKELESETFLKIIRGESELSSFDDFVKEWQEEG
                     GNEIIQEMIQERKSLSSQENQKTEEKAEQKAE"
BASE COUNT          633 a          653 c          579 g          815 t
ORIGIN      
        1 gttttacggt tgatgacaat cgcaagaatc ataccaaaaa tatagttcag gaaggtagcg
       61 agcacagccc agatcagtgt ccatccaaga acagaccaga atgtcttgcc gatcgagttg
      121 cccatgccaa gaaccgtact gaaatttttc agtccaaccc agtggaaaat cgtcagatga
      181 tcgccgactt tactgtagct ggtgaaagcc atacagatca taaagaccag tggaaggatt
      241 gtgaaaatca caataccgag aatcggaagt gtcagcaggg tccagtgcag tctcttgtca
      301 acgagtgctt ccagatcctc tttgatggtg ttgatatgtt tcccttcctt tgcacgacac
      361 tctacctcat aagcgctctt catattgaca cgccagatca taaagaagag aaccgtaata
      421 aaaatcgttg ctactccgta gagcaggaac agaattgtca tatcgccgcc ggtatattca
      481 taaataccct tggcctcgtt ccacacctct tcctgcgcac ggccgcccag ggtgatgaga
      541 tgatagagat tgttgatacc gccttcgatc ataaacagga gatacgcaat ttctactgcc
      601 agaaataaaa taccctttac gatctgtttg tgagccagat ttcccagtcc catgacaagc
      661 atggaaagtt tcgtgaccgc atcgcccttt gtgatggctt ttgtcaccgg atagggagtc
      721 tcaaactctt tggcgcgttt tttcaatgat gccataatct atcctttccg aaagacaggc
      781 attgtcaatc ctggtgtctg acaatgcctg cggtttcaat cctgttgatt ttcgaacgct
      841 tcagagccgg ttttacttac tcgactgcag aagtattcat gcttgtgttc agtttctctg
      901 tcatttctgc agcgttatca tgggtaactt caccgttgac cagagattta ccaaaattct
      961 ctgccggagt ccagtagttg ctcatattct taacgaacgg ctggatgata gaagtattgt
     1021 tgaaggtatc gttctgagca gttaccagtg catcgtctgt gatctgggaa agcagctctt
     1081 cgttacacgg aactacgttt ctcatttcat agtgtgcttt ctgagcatct gcgcttccga
     1141 gatatcttgc aagagcaact gcaacctgcg gatattcaca gttcggattc acaccgattg
     1201 ctttagaacc ggagaaggat ttcatctgtt tctcctcgcc gttgatggtg atggtcggaa
     1261 gctgtacaac accgtaatta tcaccgagtg cctctttaac tgcggatgca tcccaggaac
     1321 cggagaacat tgcttttaca gaaccgtcac gcagaccgga aagaccggat ccgtctgcat
     1381 cagatacgaa gttcggattg tttaccaggt caaccaggta atcggtcact gcttttgctt
     1441 tatcgccttc gaagtcaacg cctgctgcct cgtctgttcc gtcctcaccg aacagcgtac
     1501 agccgtttgc tacgtagaag gaagcaatgt accaggagtt tgtaatcggg aaggaaatct
     1561 tgtcttttgc gatcatggta tccaggtttt tcacttcatc ctcagtgaat gcagatttat
     1621 catagtacat aaaccaggtg tttgtggtga atggtacacc gtagatatcg ccgtcaacgg
     1681 atacggaatc tacaattgcc tgagagttgt tctcttttac ctcctcaact gtctgtccgc
     1741 cgagttttga aattccgttt gcagcgatca gggatgtcag gttgtcgttt gcaaacatgt
     1801 aaacatctgc tgctccttca acgtcctgtg ttacggtatc ttttgcatcg ctctctgcgc
     1861 atacaccgta atcaaaggta atgtgccagg atggatgctc ctctgcaaac tgattgcaca
     1921 tggtctgcag ccactcaccg ctgtcgcttg cctgatcctc tgatggagac catacggtaa
     1981 gggttgtggt aatttcctct tcctctgctg cgttggaagc agatgcgctt gtgcttcctg
     2041 cctcggatgt gctcttggaa tcgtttccgg aagcggcatt tgaagaaccg ctgtcgctgt
     2101 ttccgcatcc tgccagcatg ccaactgcaa gtgctgcggt catcataata gatactactt
     2161 tctttttcat attgaaaatc ctccttatat gatttttctt tctggaacgt ctgctgtcat
     2221 atcgcatttg ctgcttggta tctctcgttc ttattgattt tattatagca gttggcggat
     2281 ttttctgaaa tatcaagaat ctatgatcta tgttggattt ctacgttaca tttctacacc
     2341 ggagcgtatc cggaaaatca tttccgcaat ctgcagaatt cttttccgca acaaaaaaag
     2401 aggccgcgca ccgtttttcc ggtgcgtcct gcctcctctt ctgtttttta ttccgccttc
     2461 tgctcggctt tttcttctgt cttctgattt tcctgtgaac tcaggctttt cctttcctga
     2521 atcatctcct gaatgatctc atttccgcct tcctcctgcc actcttttac aaagtcgtca
     2581 aaagaagaaa gctcagactc gccccggata atttttaaaa aggtctccga ttccagttct
     2641 tttaaggtgt aacgatatgt ttctgtggtc ttcgtccggg
//
LOCUS       DAOMUF010000235         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11095_length_2648_cov_3.204396, whole genome shotgun sequence.
ACCESSION   DAOMUF010000235 DAOMUF010000000
VERSION     DAOMUF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11095_length_2648_cov_3.204396"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PLN77_09915"
     CDS             complement(<1..127)
                     /locus_tag="PLN77_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRM44620.1"
                     /translation="MSSKITIIGAGSVGSTIAYTLSNEDIASEIVLIDINKKKVEG"
     gene            complement(631..2181)
                     /locus_tag="PLN77_09920"
     CDS             complement(631..2181)
                     /locus_tag="PLN77_09920"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRM44621.1"
                     /translation="MKQSVLEYKKLLEQEGLLVEFAEPEGKEGEDRPVTLVTYFSGGA
                     DQGTLFLCKGAAFKEAYLKDAVNRGAVAYVSEKKYEAGKEIPALIVNDARKAQFVLGE
                     AFYDYPAKSLHVTALTGTKGKTTTAYYLQSMLDACLKKQGGNGCALLSSIEYYDGKTR
                     EASTLTTPESLELCRHMRNAVDAGLSHMVMEVSSQALKYERVGNTHFDTAIFLNISED
                     HISPTEHKDFDDYFQSKLKIFDRCTCACVNLDSDHIEEILKAAAKCERVVTFGSKENA
                     VIRVSDIKPGRGKFYFHVAGPDFSEDFTLPVHGAFNVENAVAAIAAAWSMGIPVSLIK
                     ESLLCVTVPGRMETYESSAGEVTAIVDFAHNGLSFEKMFDTVALEYPGSRVSVVFGTP
                     GGKAFNRRKDMGEISGKRADRIFLVPDDPGNEDPQAICEEIGGYIAKEGASFQIRMDR
                     GAAIREAILGAVPGEVVLILGKGADVTMHVGNGNVPYRSDAVYAKEALAEYGNAFAEA
                     ATARETEE"
     gene            complement(2283..>2648)
                     /locus_tag="PLN77_09925"
     CDS             complement(2283..>2648)
                     /locus_tag="PLN77_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44622.1"
                     /translation="MFLWSRQISLLFVDASETVILDQARQMLLINMVFYIPLTLVNVV
                     RFTVQGMGFSKFAILAGVCEMIGRSVVAFFLVPYFGYTAVCFASPVAWILADLFLVPA
                     YLYCAKTLRSLFEKNAQMM"
BASE COUNT          584 a          705 c          568 g          791 t
ORIGIN      
        1 ctccctctac ttttttctta ttaatatcga tgaggacgat ttcagatgcg atatcttcgt
       61 tcgacagtgt ataggcgatc gtagatccaa cgcttccagc tccaatgatg gtaatcttgc
      121 tgctcataaa tactcctttc taaaataccc ctagttggtt tttgtgattg ataaattttt
      181 cactttcctt atcatagcac tatgacaaga atttaacaag tgtttttttc gagcattttc
      241 gtgaaatctt ttttctcact gagcccgtac gcttccagaa ggggtgttct ggatatattt
      301 caatacaatt ataccatttt ttgttctttt tcacaagaat tctttccaaa aacttacagc
      361 tttgtccttc atcccgcaga attcgtcttt ttccgcatgg ttcccgggtt tttgctttct
      421 gtgttcagtt tcacatttct aactttcgtt cgttatattt ttaacaactt ttcgtcatat
      481 gtgttagttt ctgtccttct tttctacatt ttgtaggatg tatccgccaa atttcctgtc
      541 tgattttcat ttctgctgcc gccagacgca aaaaagacac ggatttttct gttccgtgtc
      601 ttttttgctc tgttttttct gtttttgctt ctattcttct gtttcacggg ccgttgccgc
      661 ttcggcaaat gcatttccat attcggcaag cgcctctttt gcatagacgg catctgaccg
      721 gtacggcaca tttccgtttc cgacgtgcat cgtcacgtcc gcgccttttc caaggatcag
      781 aacaacctct cccggtacgg cgccaagaat cgcctcccgg attgctgctc cgcggtccat
      841 gcggatctgg aaggatgcac cttcctttgc tatgtatccg ccgatctcct cacagatcgc
      901 ctgcggatct tcgtttcccg gatcatccgg tacgaggaaa atccggtccg cccgttttcc
      961 ggaaatctca cccatatctt ttctgcggtt gaatgctttt ccgcccgggg ttccgaacac
     1021 aacgcttacg cggcttcccg ggtattcgag tgctacggta tcaaacattt tttcaaaact
     1081 caggccattg tgcgcgaaat ccacgatcgc cgtgacctcg cccgcgctgc tctcatacgt
     1141 ctccatcctt cccggaacgg tgacgcaaag gaggctttcc ttgatcagcg aaaccgggat
     1201 tcccatcgac caggcagccg caatggcagc tacggcattc tccacattga aggctccgtg
     1261 caccggcagg gtaaaatctt ccgagaaatc cggtcctgcc acatggaaat agaattttcc
     1321 tctgcccggc ttgatatcac tcacgcggat tacagcattt tctttcgatc caaacgtaac
     1381 gacgcgctca cattttgccg cagctttcag gatttcctcg atatggtcgc tgtccaggtt
     1441 cacgcaggcg caagtgcagc ggtcgaagat ctttaatttc gactggaaat aatcatcaaa
     1501 atccttgtgc tccgtcgggc tgatgtggtc ctcggagatg ttcaggaaaa tggcggtatc
     1561 aaaatgcgtg tttccgactc tctcgtattt gagcgcctgg gaggatactt ccatcaccat
     1621 atgggaaagg cctgcatcta cggcattgcg catatggcgg cacagctcca gggattccgg
     1681 cgtggtgagc gttgatgctt ctctcgtctt tccgtcgtaa tattcaatcg aacttaacag
     1741 tgcacatccg tttccgccct gttttttcag gcaggcatcg agcatcgact gcaggtagta
     1801 ggctgtcgtt gtctttcctt ttgttccggt cagcgccgtg acatgcagcg actttgccgg
     1861 ataatcgtaa aacgcctcac ccagcacaaa ctgtgctttt ctggcatcgt tgacgatgag
     1921 tgccggaatt tcttttcccg cttcgtattt tttttcactt acataggcaa cagcaccgcg
     1981 gtttacggcg tcttttaagt atgcctcttt gaatgcagct cctttgcaga gaaacaacgt
     2041 tccctgatct gctccgcccg aaaaatacgt tacgagggta acgggtctgt cctctccttc
     2101 ttttccttcc ggctctgcaa attccaccag aaggccttcc tgctccagaa gtttcttata
     2161 ttctaatact gactgtttca taggatatct tcctctcttc tatgggattc tttttcagct
     2221 ttcctaaaat ccaggctcca ttataccaga attgaaacag cttgtccatc ttgcggcacg
     2281 gcttacatca tctgcgcatt tttctcaaaa agagaacgaa gcgtttttgc acagtacagg
     2341 tacgccggaa cgagaaacag atcggcgaga atccatgcca ccgggcttgc gaagcagact
     2401 gcggtgtaac caaaatacgg aaccaggaaa aaggctacca ccgaacggcc gatcatctcg
     2461 cagacgccgg caaggattgc aaatttgctg aagcccatgc cctgcacggt aaagcgtacg
     2521 acattgacaa gggtcagcgg aatgtaaaat accatgttga tcagcagcat ctgacgcgcc
     2581 tggtcgagga tgaccgtctc ggatgcatct acaaagagaa gcgaaatctg gcgggaccac
     2641 agaaacat
//
LOCUS       DAOMUF010000236         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11098_length_2647_cov_2.555170, whole genome shotgun sequence.
ACCESSION   DAOMUF010000236 DAOMUF010000000
VERSION     DAOMUF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11098_length_2647_cov_2.555170"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PLN77_09930"
     CDS             <1..1142
                     /locus_tag="PLN77_09930"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HRM44623.1"
                     /translation="FFRLLPIRRKSGVRIRIQKKQGLPFFYRRSLKRKAFFIGIFLCL
                     AGLYTLSQFIWNIHVEGNYANSTQTILNSLDAAGIHHGIWKKRVDCSQTASYLREQFP
                     NLTWVSAKIEGTQLVLAVKENVDGYVIEEKADDPQNLVAKKEGTVVRIITRNGIPQVL
                     PGDTCQAGDILVKGEIPLVNDSGETYDYSYVHADADIDLETTWYYYDTFPLSHTVRTY
                     QKAEKKTPVFQISAYRFFLGNLLPENFVESLRSMTGTADQKTEKKENGADEEVSLQQL
                     YLTENFALPFYFGYRQKFPFEIQKETYSEHEAKEKASQNYQKFHRNLSEKGLQISENH
                     VTIELNDTSCITKGTLRVIERAVEAQPCEMQKMSDGKETMVDEQQ"
     gene            1129..2160
                     /locus_tag="PLN77_09935"
     CDS             1129..2160
                     /locus_tag="PLN77_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRM44624.1"
                     /translation="MNSNNNMIELQMELPIEHEKKVFGQFDEHIKILERSLAVTLISR
                     DGVLKIVGPASNAARAKECLTQLLELSRRGNDITTQNVNYVISLVMEDSGSSLAEIDS
                     DCICHTVAGRPVKPKTLGQKAYVDAIREKMVVFGVGPAGTGKTYLAMAMAITAFRNDE
                     VGRIILTRPAIEAGEKLGFLPGDLQSKIDPYLRPLYDALYQIMGAESFQKNMEKGLIE
                     VAPLAYMRGRTLDNAFIILDEAQNTTPAQMKMFLTRIGFGSKVIVTGDASQKDLPSGT
                     RSGLDVAMQVLKKVDGISFCELTSKDVVRHPLVQKIVQAYDEYEKKAKPENHSGRAKS
                     TTRKRRENK"
     gene            2157..>2647
                     /gene="ybeY"
                     /locus_tag="PLN77_09940"
     CDS             2157..>2647
                     /gene="ybeY"
                     /locus_tag="PLN77_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRM44625.1"
                     /translation="MTLTFENEQDFDLGFDPEEVAAAVINGVLDQEACPYEAEVELIL
                     TDPENIRTMNREHRNIDRETDVLSFPMVDYPSPASFDFLETEEGDDCFNPDSGELMLG
                     DIVISVARARAQAEEYGHSLRREFAFLVAHSMLHLLGYDHMTPEEAAVMEAKQEEVLQ
                     RLG"
BASE COUNT          764 a          581 c          711 g          591 t
ORIGIN      
        1 attttttccg cctcctgccg atccgccgca agagcggtgt gcgcatccga atacagaaaa
       61 aacagggact gcctttcttt taccggcgaa gtttaaaaag aaaggcattt tttattggca
      121 tttttctctg cctggcggga ctttacacgc tgtcgcagtt catctggaat attcatgtgg
      181 aaggaaacta cgcgaacagt acgcagacga ttttaaatag cctggatgcg gccgggattc
      241 atcatggaat ctggaaaaaa cgagtggact gcagccagac cgcatcgtat ctgcgggagc
      301 agtttcctaa tctgacctgg gtgtccgcaa aaatagaagg gacgcagctt gtccttgcgg
      361 taaaagaaaa cgtagacggc tatgtgatcg aagaaaaagc agatgatccg cagaatcttg
      421 tggcgaaaaa agaagggacg gtcgtgcgta ttatcacacg gaacggcatt ccgcaggtgc
      481 tccccggtga tacatgtcaa gcgggagaca ttcttgtaaa gggcgagatt ccgcttgtga
      541 atgacagtgg ggaaacgtat gattattctt atgttcacgc agacgcggat attgaccttg
      601 agacgacgtg gtactattat gatacctttc cgctttccca tacggtacgg acatatcaga
      661 aagcagagaa aaaaacgccg gtattccaga tttctgctta ccgatttttc cttggaaatc
      721 tgctgccgga gaattttgtg gagagtttgc ggagcatgac cggaacggcg gatcagaaaa
      781 cagagaaaaa ggaaaatgga gcggacgagg aagtgtccct tcagcagctt tatctgacag
      841 aaaactttgc ccttccgttt tatttcggct accgtcagaa atttcctttt gaaattcaga
      901 aagaaactta ctcagaacac gaggcaaaag aaaaagcctc gcagaattat cagaaattcc
      961 acagaaattt atcagaaaaa ggacttcaaa tatcggagaa tcatgttaca atagaactta
     1021 acgatacgtc atgtatcaca aaagggacac ttcgcgtgat agaacgggcc gtagaagcac
     1081 agccctgcga aatgcagaag atgtcggatg gaaaggaaac aatggtagat gaacagcaat
     1141 aacaacatga tagaactgca gatggaactg cccatcgaac atgaaaagaa agtattcggc
     1201 cagtttgatg aacacatcaa aatcctggag agatccctgg cggttacgct gatctcccgg
     1261 gacggcgttt taaaaattgt aggcccggca tcgaatgcag cgcgcgcaaa ggaatgtctg
     1321 acacagcttc tggagctttc acgccgcgga aatgatatca ccacacagaa cgtcaattat
     1381 gtaatctcac ttgttatgga ggacagcgga agcagccttg ctgaaatcga cagtgactgc
     1441 atctgccaca cggtcgcagg ccgtccggtc aaaccgaaaa cactcggtca gaaagcgtac
     1501 gtggatgcga ttcgggaaaa gatggtcgtc ttcggcgtcg gccctgccgg aaccggaaaa
     1561 acataccttg caatggcgat ggcgatcacc gcgttccgaa acgatgaagt aggacgtatt
     1621 atcctgacgc gtccggcgat tgaggcgggt gaaaaactcg gtttcctgcc tggcgacctg
     1681 cagagtaaaa ttgacccgta tctgcggccg ctctacgacg cgctgtatca gatcatggga
     1741 gcagagagct tccagaaaaa catggaaaaa ggcctgattg aggttgcgcc gctcgcgtac
     1801 atgcgaggcc gtacgctcga caatgcgttt attatccttg atgaggcaca gaatacgaca
     1861 ccggcgcaga tgaagatgtt cctgacccgt atcggtttcg gttccaaagt cattgtaacc
     1921 ggagatgcct ctcagaagga ccttccttcc ggtacacgct ccggccttga tgtggcaatg
     1981 caggtactga aaaaagtaga cggaatttcc ttctgcgagc tgacgagcaa ggacgttgtc
     2041 cgtcatccgc tggtgcagaa gattgtacag gcttacgatg aatatgaaaa gaaagcaaag
     2101 ccggaaaatc acagcggcag agcaaaaagt acgacgagaa aacggaggga aaataaatga
     2161 cactgacgtt tgaaaatgaa caggattttg accttggatt tgatccggaa gaggttgcag
     2221 cagcggtaat caatggtgtg ctggatcagg aagcgtgccc gtatgaggca gaagtagaac
     2281 tgattctgac cgatccggaa aatatccgca ccatgaaccg ggaacacaga aacattgacc
     2341 gggagacaga cgtgctttct ttcccgatgg tagattatcc ttctccggct tcgttcgatt
     2401 ttctggagac ggaagaggga gatgactgtt ttaacccgga cagcggtgag ctgatgctgg
     2461 gtgatatcgt aatttctgtt gcgcgcgcaa gagcgcaggc ggaggaatac ggtcattctt
     2521 taagacgcga atttgctttt cttgttgcac acagtatgct gcatcttctg ggttatgatc
     2581 atatgacacc ggaggaagca gccgtgatgg aggcaaagca ggaagaggtg ctgcagcgtc
     2641 tcggtat
//
LOCUS       DAOMUF010000237         2601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11362_length_2601_cov_4.609976, whole genome shotgun sequence.
ACCESSION   DAOMUF010000237 DAOMUF010000000
VERSION     DAOMUF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11362_length_2601_cov_4.609976"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="PLN77_09945"
     CDS             <1..1835
                     /locus_tag="PLN77_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRM44626.1"
                     /translation="LRLIAGDIEMSNLDSDESCGITMSGKQNIGFLRQINFTDGDVPV
                     KEELEKAFSEVFACEARKKEIEKLLAETEDHKKLLAEYDFLERRIESLHGYSWKKDME
                     TMFQKFGFALEDLEKPIGEFSGGQQTKIALIQLLLQRPDILLLDEPTNHLDIAAVEWL
                     ENYLKSYENAVIIVSHDRMFLDRVIDVTYEIEYGDIKRYPGNYSAFVKQKEEAQIKQE
                     KDYEAQQKEIERLTAWIEKWKNTPTKVAATRSKRMAIEHMVKIEKPRRFDTRTFHALF
                     YPVRESYTDVLTMKKLKIGYDTVLSEVSAVIKKAERIVIIGENGKGKSTLLKTVVGEI
                     NPLGGSYQFGNRVDFAYFDQHKAVNQKFDPEQTVLDYFWSLYPNLLRNDVRSALGAFL
                     FSGEDVEKKMGQLSGGEKVRLELCRIFYTKPNFLILDEPTNHMDLAGKEALEKMLTSY
                     EGTVLFVSHDRYFINQVATGILEFGESDVQFYGMNYAQYLEEIKRQTPGRETGNAGGV
                     VRKPADVPTLDDVFDKKKYYNPGKILSRLKRQLEKYEEQLAESESRMSELQLEMMDPE
                     LSANYEQLMELQTKIDEENQVQESLLERMMETELELEEMETQDE"
     gene            2296..>2601
                     /locus_tag="PLN77_09950"
     CDS             2296..>2601
                     /locus_tag="PLN77_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HRM44627.1"
                     /translation="MENFINNAPFALVLVCLVIGFVLLIKGADFFVEGSSSAAKRLHV
                     PSIIIGLTIVAMGTSLPETAVSVSASLTGNNELAVSNVIGSNIFNLMVVIGVCAVLTT
                     "
BASE COUNT          795 a          529 c          673 g          604 t
ORIGIN      
        1 tgctgcggct gattgccggg gatattgaga tgagtaatct ggacagcgac gagagctgcg
       61 gaattacgat gagcggaaaa cagaacatcg gttttctgcg ccagattaat tttaccgatg
      121 gagatgtgcc ggtaaaagag gagctggaga aggctttctc agaagtcttt gcgtgcgagg
      181 cacgaaaaaa ggaaatcgaa aagctgctcg ccgagacgga agatcacaaa aagctgctgg
      241 cggagtacga ttttctggag cggagaatcg agagtctgca cgggtacagc tggaaaaaag
      301 atatggaaac gatgttccag aaatttggtt ttgcgctcga agatctggaa aaaccgatcg
      361 gtgaattttc gggcggacag cagacaaaaa ttgccctaat tcagctcctc ttacagcgcc
      421 cggacatcct gcttctggac gagccgacca accacctcga catcgctgca gtggagtggt
      481 tggaaaatta tctgaaaagc tacgaaaatg ccgttatcat tgtatcacat gaccgcatgt
      541 ttctggaccg cgtgattgat gtgacgtatg aaatcgaata cggtgacatc aaacggtatc
      601 cgggaaatta ttcggcattt gtgaaacaaa aagaagaagc acagatcaaa caggaaaaag
      661 actatgaagc gcagcagaag gaaatcgagc gtctgacggc gtggattgaa aaatggaaaa
      721 acacaccgac aaaagttgcg gccacccgtt cgaaacgaat ggcgatcgag cacatggtaa
      781 aaattgagaa accgcggcgg tttgacacga ggacattcca cgccttattc tatccggtac
      841 gcgaaagcta tacggatgtg ctcacgatga agaaactgaa aatcggatac gataccgtcc
      901 tgagcgaggt ttcggccgtc atcaaaaaag cagaacggat tgtgatcatc ggagaaaacg
      961 gaaaaggaaa atcgacgctt ctgaaaaccg ttgtgggcga aatcaatcca ctgggcggaa
     1021 gttatcaatt tggaaaccgc gtggattttg cctacttcga tcagcacaaa gcagtcaacc
     1081 agaaattcga cccggagcag acggtgctcg actatttctg gagtctttat ccgaacctgc
     1141 ttcgcaacga tgtccgaagc gcactgggag cctttctgtt ttccggtgag gatgtcgaaa
     1201 agaaaatggg gcagctctcc ggcggtgaaa aagtgcggct ggagctctgc cggattttct
     1261 atacgaaacc gaatttcctg atcctggacg agccgacgaa tcacatggat ctggcgggaa
     1321 aagaagcgct ggaaaaaatg ctgaccagct acgagggaac cgttctgttc gtatcgcacg
     1381 accgttattt catcaaccag gtggcaaccg gcattctgga gttcggcgag agcgacgtac
     1441 aattttacgg aatgaattat gcacagtatc tcgaagaaat caaacggcag acgccgggca
     1501 gagaaacggg aaatgcggga ggagttgtac gaaaaccggc cgacgttccg acactggatg
     1561 atgtgtttga caaaaagaaa tattataacc cgggaaaaat tctgtcccgc ttaaaacggc
     1621 agctcgaaaa atacgaggag cagcttgcgg agagtgagag cagaatgtca gaacttcaac
     1681 tggaaatgat ggacccggaa ctttccgcaa attacgaaca gctgatggag cttcagacga
     1741 aaatagacga agaaaatcag gtacaggaat ccctgttgga gcgcatgatg gaaacggaac
     1801 tggaactcga agagatggaa acacaggacg aatagagagg tgagtaccat agaaaagata
     1861 gatcagtcta aaaatggact atcatgaaaa tgaagttttt ctcgttttag gatattcggt
     1921 ttgttatatt ttgtaaagta ttctgcaccc atgactggta tgcattcgta agcggggctg
     1981 aaatctttcg gataaaaaag tcaaattttg ttgacaaccc cgaagacatt tgatagtatt
     2041 tatcaagtaa tgtaatattc catgagggag tagttagcac gaatgtgtga tttgtcaaca
     2101 ttctgattat aagaaaagag tataatctgg caaatcttaa ataatgagac tcatgtatat
     2161 cgatgaatca ccggtgtatc gcatatcgga tgttccttga tatacatgag tctttttttg
     2221 cggataatga gtaaaacctg cttctgttaa cgatactatt ttggaatata tgcttggaaa
     2281 acaaaggagg aaaacatgga aaatttcatc aacaacgccc cgtttgctct tgtgcttgtc
     2341 tgtctcgtca tcggttttgt cctgctgatc aaaggtgcgg actttttcgt agaaggaagc
     2401 tcaagcgcag caaagagact gcacgttccg tcgatcatca tcggactgac cattgttgcc
     2461 atgggaacat cacttccgga gaccgcggtc agtgtatcag cttcacttac cggcaacaac
     2521 gagcttgccg tcagtaatgt catcggttcc aatatcttta acctgatggt tgtcatcggt
     2581 gtctgtgccg tactgaccac c
//
LOCUS       DAOMUF010000238         2592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11422_length_2592_cov_3.076862, whole genome shotgun sequence.
ACCESSION   DAOMUF010000238 DAOMUF010000000
VERSION     DAOMUF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11422_length_2592_cov_3.076862"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLN77_09955"
     CDS             complement(<1..923)
                     /locus_tag="PLN77_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM44628.1"
                     /translation="MLRRYKLKNYNFRLVILLIAISIIGVLLIGSADSADQKKQAIGV
                     VLGVVIMIIVSLLDYSWLLNFYWVLYIGNLMMLGVLYLGGSTAKNTFGATRWIQIGSF
                     QFQPTELSKIFLIMFFAMFLMKHEEDLNTAKTIFTAIGLLIPPLVMIYRQPDLKNTIT
                     VMVLFCFMMYVAGLSYKVIAVVLVSGSALVLALLLVITQVNFQLPAALDKTIGYQLGR
                     IQDWLSDDADNADGRLQQQNSITAIGSGKLTGKGLNNNKVSSANKGNFVSQNHNDFIF
                     AVAGEELGFFGCCGIILLLFLILVECIRTSR"
     gene            complement(907..1167)
                     /locus_tag="PLN77_09960"
     CDS             complement(907..1167)
                     /locus_tag="PLN77_09960"
                     /inference="COORDINATES: protein motif:HMM:NF015717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRM44629.1"
                     /translation="MGLFSGRKPHSGLVAKERLSNLLMAERLNCSPASLQMLKNDLSA
                     AAGKYLTVEADEISVTITQTTPVILTAKIPLKRNEEYHVKTI"
     gene            complement(1180..1959)
                     /gene="minD"
                     /locus_tag="PLN77_09965"
     CDS             complement(1180..1959)
                     /gene="minD"
                     /locus_tag="PLN77_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRM44630.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANIGIGLAQLGKKVVMVDTDIGLR
                     NLDVVLGLENRIVYNLVDVVEGNCRMKQALIRDKRYEQLYLLPSAQTRDKTSVTPEQM
                     KKLCDELKQSFDFVLLDCPAGIEQGFANAVAGANKAIVVTTPEVSAIRDADRIIGLLE
                     AQDLRDISLVINRIRPDMVKRGEMMSVDDVQEILAVPLLGILPDDEQVVIATNQGEAI
                     AGSGSKAGEAFTNISHRILGESVPYLDLDSKKGFFRRVFGR"
     gene            complement(2089..>2364)
                     /locus_tag="PLN77_09970"
     CDS             complement(2089..>2364)
                     /locus_tag="PLN77_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44631.1"
                     /translation="TIFPESWPVELSGKTGTAEEDHTRANHGLFMGFSHYESRDDIAL
                     AVRIPFGYSSMNAELVAKDILDYYYGLSDDILSGQANSNGVASVRAD"
     assembly_gap    2365..2374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2375..>2592)
                     /locus_tag="PLN77_09975"
     CDS             complement(<2375..>2592)
                     /locus_tag="PLN77_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM44632.1"
                     /translation="TTTQLARYVTTIANSGTVYNLSLLQKATDSDGNDIDMGADFGPT
                     VNSEMDVDSSIWDLVHEGMRKVISVSNA"
BASE COUNT          668 a          666 c          578 g          670 t
ORIGIN      
        1 ttccgggaag tccggatgca ttcgacgaga atcaggaaca ggagcaggat gattccgcag
       61 catccaaaga aaccaagctc ctctcctgcc actgcaaaaa taaagtcatt atggttctgg
      121 gaaacgaaat ttcccttatt tgcggaagaa actttattgt tgttgagtcc ttttccggtc
      181 agctttccgg aaccgatggc tgtaatggaa ttctgctgct ggagacggcc gtcggcgttg
      241 tcggcatcat cgcttaacca gtcctgaatt cgcccaagct gataaccaat cgtcttgtca
      301 agagccgccg gaagctggaa attcacctgc gtaatgacaa gcagaagcgc aagtaccagt
      361 gctgaaccgg aaaccagaac aacggcgata actttgtagc ttaagcccgc cacatacatc
      421 ataaagcaga acagaaccat gaccgtaatc gtatttttca aatccggctg acggtaaatc
      481 ataacgagcg gcggaatcag aagcccgatc gcggtaaaaa tggttttcgc cgtattcaga
      541 tcttcttcat gcttcatcag aaacatcgca aaaaacatga tcagaaagat ttttgacagc
      601 tcggtcggct gaaactgaaa gcttccgatc tgaatccaac gggtcgcacc aaacgtgttt
      661 tttgcggtag aaccgccgag ataaagaacg ccaagcatca tcagatttcc gatgtaaaga
      721 acccagtaaa aattcagaag ccagctatag tcaagcaaag agacaataat catgatgact
      781 acgcccaata cgactccgat ggcctgtttt ttctggtctg cactgtctgc acttccgatc
      841 agaagcacac caatgatgct gatggcaatc agaagaatga ccagccggaa attataattt
      901 ttcagcttat atcgtcttaa catgatactc ctcgtttctt ttcaatggta ttttcgctgt
      961 cagtatgaca ggggttgttt gtgtaatggt gacggaaatt tcatccgcct caacggtaag
     1021 gtattttcct gcagcagcag aaagatcatt tttcagcatc tgaagcgatg ccggagaaca
     1081 gttcagacgt tctgccatta aaagattgga cagacgttct tttgcaacga ggccggagtg
     1141 cggctttctg cctgaaaata aacccatacc agttccctcc tatcgcccga atacgcggcg
     1201 gaaaaatccc tttttgctgt caagatccaa gtagggaaca ctctccccga ggatcctgtg
     1261 gctgatattc gtaaaggctt ctcctgcctt ggaaccgctt ccagcgatcg cttcgccctg
     1321 gttggtggcg attactacct gctcatcatc gggcagaatg ccgagcagcg gaacagcaag
     1381 aatttcctgt acatcatcca ccgacatcat ttcaccgcgt tttaccatat ccggccggat
     1441 acgattgatg acaagagaga tatccctcag atcctgcgcc tccagaaggc cgatgatgcg
     1501 gtccgcgtcc cggatggccg aaacctccgg cgtagtcaca acaattgcct tattggcacc
     1561 ggcaactgca tttgcaaatc cctgctcaat gcctgccgga cagtcaagaa gaacaaaatc
     1621 aaagctctgt tttaattcat cgcagagttt tttcatctgc tcgggcgtta cgcttgtctt
     1681 gtccctcgtc tgggcagacg ggagaaggta gagctgctcg taccgtttgt cgcggattag
     1741 cgcctgcttc atccggcagt tgccctccac aacatccaca agattgtaga caatccggtt
     1801 ttcaaggccg agcacgacat ccaggtttcg aagaccgatg tcggtatcta ccatcacaac
     1861 tttctttcca agctgtgcca gcccgattcc gatatttgct gcggttgtcg tctttccgac
     1921 gccgcctttt cctgatgtaa ttacaatgac ttcactcatc tggaacctcc ttgttgaaaa
     1981 ctgttctgca ttttcatgcc gcaggatgcg tccaaacctt tgtttaaaaa cacatccttt
     2041 ctcttttgca gctttcttcc caggcttcgt ttctgccgga aaaattcctt agtctgctct
     2101 cacgcttgca acaccgttgg agtttgcctg accggacaga atatcgtctg agaggccata
     2161 gtaataatct aagatatcct tagctaccaa ctctgcgttc atagaagaat atccaaacgg
     2221 aatgcggacg gcaagagcaa tatcgtctct gctctcatag tgagagaagc ccataaacag
     2281 tccatggttt gcacgtgtat ggtcttcctc cgcggtaccg gtttttccgg aaagctctac
     2341 cggccaggat tccgggaaaa tggtnnnnnn nnnncgcatt ggatacgctg attacttttc
     2401 gcatgccttc gtgtaccaga tcccagatgc tggaatcaac atccatttct gaatttaccg
     2461 tcgggccgaa atctgcgccc atatcgatgt cattgccatc cgaatccgtt gctttctgca
     2521 gaagggaaag attataaacc gtaccggaat ttgcaatcgt tgtgacatag cgtgcaagct
     2581 gtgtcgtcgt at
//
LOCUS       DAOMUF010000239         2588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11450_length_2588_cov_2.128701, whole genome shotgun sequence.
ACCESSION   DAOMUF010000239 DAOMUF010000000
VERSION     DAOMUF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11450_length_2588_cov_2.128701"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLN77_09980"
     CDS             complement(<1..365)
                     /locus_tag="PLN77_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44633.1"
                     /translation="MLRIWQKTEKQKLEPVGMGTAFPFLKEKNHVVSIIGGGGKTTLL
                     YEMAGFCVKNDQKVLVTTSTHIYRPPKEWHDQSLEAVERKFRTGRAAIIGSACRDPEK
                     LSMPETELFEEARKKADLTL"
     gene            complement(504..>882)
                     /locus_tag="PLN77_09985"
     CDS             complement(504..>882)
                     /locus_tag="PLN77_09985"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRM44634.1"
                     /translation="ATQETDNSGAADESAPAAEEPQQNDTEGTKNETDAQMEIEALPA
                     ESPKLYLNTYEVTVEAGNDLDKTSYIKEMTDDADSQESLFNQIEIDGDVNTAVPGDYT
                     LTYHVTDSDGNRSNAAVLTVHVQ"
     assembly_gap    883..892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<893..1227)
                     /locus_tag="PLN77_09990"
     CDS             complement(<893..1227)
                     /locus_tag="PLN77_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44635.1"
                     /translation="MKKGIVAGLIVLCVALGAGTAAVVVTDDRKGPEIELPADGDVTY
                     EEGSDTAPLLEGVTATDEKDGDVTDSLMIENIFPNDDHTSASVVYAAKDSHNNVSKAT
                     RRVNYKVAA"
     gene            complement(1233..1934)
                     /locus_tag="PLN77_09995"
     CDS             complement(1233..1934)
                     /locus_tag="PLN77_09995"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRM44636.1"
                     /translation="MNQVVVKQEKSGYQIVEAYKSLRTNLQFCGEDKKVIAVTSCTPN
                     EGKSSVSMQLGISLAESGKRVILIDADLRKSVLLGRTKTQKSVRGLAHFLSGQATLED
                     VICSTNVKNFYMIYSGPFPPNPAELLGGKNFRSLLNALRKVYDYVIVDTPPLGSVIDS
                     AIVAEICDGSIMVIESGVISYRFAQEVKSQLERSNCPLLGVVLNKVDMQKQAYGKYGK
                     YGQYKYGDYGQEEDD"
     gene            complement(1950..>2588)
                     /locus_tag="PLN77_10000"
     CDS             complement(1950..>2588)
                     /locus_tag="PLN77_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRM44637.1"
                     /translation="LGLLMVLVSKVLMKPQYESTTKMYVLSKQDSSNTVTSGDLQASS
                     LLTKDYAELIQSRQVVETVIAQLNLDLTYEQFLNKLTVTTQNDTRILSITVKDEDPYM
                     ASQIADAIRVAAADHIQNVMNTEAVNVVDEANIPDEPVSPSIKKNGLIGAVAGAFIAI
                     IVIIVVYLTNDTIQTSEDVERYLGVSTLGIIPIREGQKKNKKKNKNGRGRKK"
BASE COUNT          541 a          722 c          528 g          787 t
ORIGIN      
        1 agcgtcagat cggccttttt gcgtgcctct tcaaatagtt ccgtttccgg catggaaagc
       61 ttttccggat cccggcaggc actcccgata atcgctgcgc ggcctgtgcg aaattttctt
      121 tcgaccgctt ccagactctg gtcatgccat tccttcggcg gacgataaat gtgcgtcgaa
      181 gttgtcacca gtaccttctg atcgtttttt acacaaaagc ctgccatctc gtacagcagc
      241 gtcgtttttc cgccgccgcc gatgatagaa actacatggt tcttttcctt taaaaagggg
      301 aacgccgttc ccattcctac cggctccagc ttctgttttt ctgttttctg ccagattcga
      361 agcatctcct ctgccccctt ctgccgtctt tctttctcac tctaccatac tcattttggg
      421 ctgtcaaatt ttactcgcgc cgtaaaaact tgaaaaaaga actgccccga tcgggcatcc
      481 ccatcagagc agttcttcta ttcttactga acgtgtacgg taagcaccgc tgcatttgat
      541 ctgtttccat cgctgtccgt cacatgatac gtcagcgtgt aatctcccgg cactgcagta
      601 ttcacatcgc cgtcgatctc aatctggtta aagagtgact cctggctgtc tgcatcatcg
      661 gtcatctctt tgatatagct ggttttgtcc agatcgtttc cggcttccac tgttacttca
      721 tacgtattca ggtacagttt tgggctttcc gccggcagtg cttcgatttc catctgcgca
      781 tctgtctcat tttttgtgcc ctctgtgtca ttctgctgcg gctcctctgc tgccggtgca
      841 gactcatctg ccgccccact gttatccgtt tcctgcgtag ccnnnnnnnn nntccgctgc
      901 aactttataa ttcacgcggc gtgttgcttt tgatacatta ttgtgactgt cctttgccgc
      961 atagacaacc gatgcactgg tatggtcatc gttcgggaaa atgttttcaa tcatcagcga
     1021 atctgtcaca tctccgtctt tctcatccgt tgcagtaacg ccttccagca aaggcgctgt
     1081 atcactgcct tcctcatacg ttacatcccc atcagctgga agctcgattt ccgggccttt
     1141 tcggtcgtcc gtcactacga cagctgctgt accggctcct agcgccacgc acaggacaat
     1201 caaacctgca acaatccctt ttttcatggc agttaatcgt cctcctcctg gccgtaatct
     1261 ccatatttgt actgaccgta ttttccgtat ttaccatacg cctgtttctg catatccacc
     1321 ttgttcagga caacgccaag cagcgggcag ttacttctct cgagctggct ctttacttcc
     1381 tgtgcaaaac gatagctgat cacaccggac tcaatgacca tgattgaacc atcacagatt
     1441 tctgcaacga ttgcactgtc gataacgctt ccaagcggag gagtatccac aatcacataa
     1501 tcgtaaactt tacgaagtgc atttaaaaga cttcggaagt tctttccccc cagaagctct
     1561 gccggattcg gcggaaacgg gccggaataa atcatgtaaa agttctttac attggtagaa
     1621 cagatgacat cctccagagt tgcctgaccg gacaggaagt gtgccagtcc gcgaacagat
     1681 ttctgtgttt tcgttcttcc aagaagaacc gatttccgaa gatcggcatc gatcaaaatc
     1741 actctttttc cgctctctgc aagagaaatg ccgagctgca tagaaacact gctctttccc
     1801 tcatttggtg tacagcttgt aaccgcgatt actttcttat cttctccgca gaactgaagg
     1861 ttggtccgaa gagatttgta ggcttccaca atctgatagc ctgatttttc ctgttttact
     1921 actacctgat tcacgttccg tatcctccct tatttttttc ttccacggcc gtttttgttt
     1981 ttcttcttgt tctttttctg tccttctctg atcggaatga taccgagtgt gctgacaccc
     2041 agataacgct ctacatcctc agacgtctgg atcgtgtcgt tggtcagata aacaacaatg
     2101 ataacaatga ttgcgataaa ggctcctgct accgcaccga tcagtccgtt tttcttgatg
     2161 ctcggactta ccggctcatc cgggatattt gcctcatcta ccacatttac ggcttcggtg
     2221 ttcataacgt tctggatatg atctgctgct gccacacgga tcgcatccgc tatctggctt
     2281 gccatatacg gatcctcatc ctttaccgtg atcgaaagaa tacgcgtgtc gttctgcgta
     2341 gtaacggtaa gcttattcag aaactgctca tacgtaagat ccagattcag ctgtgcaatg
     2401 acggtttcca ccacctgacg gctctggatc agctccgcat agtccttggt cagaagggag
     2461 cttgcctgca ggtctccgct tgttacggta ttgctgctgt cctgtttgga cagtacatac
     2521 attttcgttg tcgattcata ctgcggcttc atcaatacct tgctgaccag taccatcaga
     2581 aggccaag
//
LOCUS       DAOMUF010000240         2575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11509_length_2575_cov_3.459127, whole genome shotgun sequence.
ACCESSION   DAOMUF010000240 DAOMUF010000000
VERSION     DAOMUF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11509_length_2575_cov_3.459127"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLN77_10005"
     CDS             <1..375
                     /locus_tag="PLN77_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HRM44638.1"
                     /translation="SVAQEKPTIGYPLAFSHPGAVEAQAKLGEMTLARFDGDNGEYSL
                     LLGNAKGVEGPYTKGTYVWVEVQNLKRLEAKIVEGPYIHHCVGIHENVVPVLYEACKY
                     IGIAPDLYDPIEEDVKAYLRGE"
     gene            381..1214
                     /locus_tag="PLN77_10010"
     CDS             381..1214
                     /locus_tag="PLN77_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRM44639.1"
                     /translation="MSEYKELSCLAYDLRKKVVEMVVSGKGGHIGGDMSVIDVLVALY
                     FKEMNISPKNMDDPDRDRFVMSKGHSVEAYYAVLAAKGFFPMEEVIEKFSKFGSPYIG
                     HPNNKLPGIEMNSGSLGHGLPVCVGMALAGKMDKKDYRVYTVMGDGELAEGSVWEGAM
                     SAGNYKLDNLCAIVDRNRLQISGNTEDVMKQDSQEERWAAFGWNVLSVPGNDMDALVH
                     AFELAKHCKGKPTVIIANTTKGCGSSVMENKAEWHHKVPTPEEVEQIMKDLDERKEAL
                     S"
     gene            1211..2143
                     /locus_tag="PLN77_10015"
     CDS             1211..2143
                     /locus_tag="PLN77_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRM44640.1"
                     /translation="MNKIPNRQAICEVLMKHAETDKDIVVLCSDSRGSASLTPFANAY
                     PEQFVEMGIAEQDLVSVSAGLAKCGKKAFAASPACFLSTRSYEQAKIDCAYSNTNVKL
                     IGISGGVSYGALGMSHHSAQDIAAMAAIPNMRVYLPSDRFQTAKLVEALLTDEKPAYI
                     RVGRNAVADIYSEENTPFEMDKATVLSEGTDVLLVACGEMVRPALDAAAMLKEEGISA
                     GLLDMYCVKPLDKEGLVEAAKKAKVVISIEEHAPFGGLGSMVSQVVGAECPRKVVHMS
                     LPDAPVITGTSKEVFDYYGLNAEGIAKKAKELLA"
BASE COUNT          709 a          547 c          757 g          562 t
ORIGIN      
        1 tctgttgcac aggaaaaacc gacgatcggt tatcctctgg cattcagcca tccgggggct
       61 gtagaggcgc aggcaaaatt aggcgaaatg acactggcgc gttttgacgg agacaacgga
      121 gagtactccc tgctgcttgg aaatgcaaaa ggtgttgagg gaccttatac gaagggaacc
      181 tacgtatggg ttgaggtaca gaacctgaaa cgcctcgagg caaaaatcgt ggaaggaccg
      241 tacatccacc actgtgtcgg tatccacgag aacgttgtgc cggttctgta tgaagcatgc
      301 aaatacatcg gcattgcccc ggatttatat gatccgattg aggaagatgt caaagcatat
      361 ttaagaggag aatgaaaatc atgagtgaat acaaggaatt gagttgtctg gcgtatgatc
      421 tgcggaagaa agtagtggaa atggtagttt ccggcaaggg cggccacatc ggcggcgaca
      481 tgagtgtcat cgatgtgctt gtagcgctgt attttaaaga gatgaacatc agccctaaga
      541 acatggacga cccggaccgc gaccgttttg taatgagtaa aggtcattcc gttgaggcat
      601 attatgcagt acttgcagca aagggattct tcccgatgga agaggtcatc gaaaaattct
      661 ccaaattcgg cagcccgtac atcggtcatc cgaacaataa actgccgggc atcgagatga
      721 attccggttc tctcggccac ggacttccgg tctgcgtggg aatggcgctg gcaggaaaaa
      781 tggataaaaa agattaccgt gtttacaccg ttatgggtga cggcgagctg gcagaaggct
      841 ctgtctggga gggcgcaatg tccgcaggaa actacaagct ggacaacctc tgtgcgatcg
      901 tagaccgcaa ccgtctgcag atctccggaa ataccgagga tgtcatgaaa caggacagcc
      961 aggaggagag atgggcagcg ttcggatgga atgttctttc cgtaccgggc aacgatatgg
     1021 acgcgctcgt tcatgcgttt gagctggcga aacactgtaa gggaaaaccg acggttatta
     1081 ttgcaaatac aacgaagggc tgtggttctt ccgtgatgga aaataaggcg gagtggcatc
     1141 ataaagtgcc gacaccggag gaagtagaac agatcatgaa ggatctggat gagagaaagg
     1201 aggcactgtc atgaataaga ttccaaacag acaggccatt tgcgaggttt taatgaaaca
     1261 tgccgaaacg gataaagata tcgtggtgct ctgcagcgat tccaggggaa gtgcttccct
     1321 gacaccgttt gcaaatgcgt atccggagca gtttgtggag atgggtatcg cggagcagga
     1381 tctggtcagc gtttctgccg gtcttgcaaa atgcggcaag aaagcatttg cggcatctcc
     1441 ggcgtgcttc ctctccacca gaagctatga gcaggcgaag attgactgtg catactccaa
     1501 caccaatgta aagctgatcg gcatcagcgg cggtgtgagc tacggcgcac tcggcatgag
     1561 ccatcattct gcacaggaca tcgcagcaat ggcagctatt ccgaacatgc gcgtttatct
     1621 gccgagcgac cgcttccaga cagcaaaact ggttgaggcg cttcttaccg atgaaaaacc
     1681 ggcttatatc cgagtgggaa gaaatgccgt tgcggatatt tacagcgaag aaaatacccc
     1741 gtttgagatg gataaggcaa cggttctctc ggaaggcacc gatgttttac tggtagcctg
     1801 cggtgagatg gtccgcccgg cgctcgatgc agctgctatg ctgaaagaag agggaatcag
     1861 cgcaggactg ctcgatatgt actgtgtgaa accgctggac aaagagggcc tggtagaggc
     1921 tgcaaagaag gcaaaagttg tcatttcgat cgaagagcac gctccatttg gcggccttgg
     1981 atcgatggtg agccaggtgg tcggtgcgga atgcccgcgc aaagtcgttc atatgtctct
     2041 gccggatgcg ccggtgatca cgggaacttc caaagaagtc tttgactatt atggactgaa
     2101 tgccgagggc attgcaaaaa aagcgaaaga attactggct taaccagaaa tggaatcagc
     2161 ggtgaatgtg atagcccgca gggaactgta aactgccgca ggtctgcgta tgccatgttc
     2221 ctgcgtgcct gggcggaaga aaatccggga acgcaggaac tttttctata taatcaggaa
     2281 taaatcgttc tgcggaagat gttggaatgt gtggtaatcg ccaaagatct tgcctatagg
     2341 ggcagggtga atgccgcgac aatagaaaat gacgcatggt gtgttgaaaa acagggtgta
     2401 ttcagcgtta ttcgagatgg ggatctggat atttataaaa ttttaggaat ggagctggaa
     2461 aaatggagaa aaggtatatc atcggaattg accagagcac gcagggaacg aaagcgctgc
     2521 tctttgatgg ggagggacat ttactccgcc gtaccgacct gccgcaccgg cagat
//
LOCUS       DAOMUF010000241         2556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11613_length_2556_cov_2.604958, whole genome shotgun sequence.
ACCESSION   DAOMUF010000241 DAOMUF010000000
VERSION     DAOMUF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11613_length_2556_cov_2.604958"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLN77_10020"
     CDS             complement(<1..470)
                     /locus_tag="PLN77_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM44641.1"
                     /translation="MFQYHCLNPIAGVGLANFNENYQQIESLEGADAVLVRSAAMHGM
                     ELPKSLLAVARAGAGVNNIPLTDCAEQGIVVFNTPGANANGVKELVLAGMLLASRDIA
                     GGIEWVKSDKEDGDIAKTAEKQKKKFAGCEISGKKLGIIGLGAIGQLVGNAATHL"
     gene            complement(470..1552)
                     /gene="serC"
                     /locus_tag="PLN77_10025"
     CDS             complement(470..1552)
                     /gene="serC"
                     /locus_tag="PLN77_10025"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRM44642.1"
                     /translation="MSRVYNFSAGPAVLPEEVLKEAQEEMLDYRGCGMSVMEMSHRSK
                     VFQNIIDEAEADLRDLMGIPSNYKVLFLQGGASLQFSMIPMNLMKNGVADYIVTGQWA
                     KKAYAEAQKYGKANKIASSEDKTFSYIPDCSDLPISPDADYVYICENNTIYGTKYKKL
                     PNTKGKTLVADVSSCFLSEPVNVSDYGIIYGGVQKNIGPAGMVISIIRDDLITDDVLP
                     GTPTMMKFKTHADAGSLYNTPNCYCIYMCGKVFKWLKKMGGLEVMKQRNEEKAKLLYD
                     FLDQSKLFKGTVVPEDRSLMNVPFITGNADLDAKFVKESKEAGLENLKGHRTVGGMRA
                     SIYNAMPKKGVETLVAFMKKFEEENL"
     gene            complement(1880..>2556)
                     /locus_tag="PLN77_10030"
     CDS             complement(1880..>2556)
                     /locus_tag="PLN77_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRM44643.1"
                     /translation="IAKIREMKEKYPLKHEEGLTGPYVIEEIYKATDGQAIITTDVGQ
                     HQMWAAQYYRYTSPRTLLTSGGLGTMGFGLGAAMGAKMGRPDKTVINIAGDGCFRMNM
                     NELATLSRHNIPVIEVVVNNHVLGMVRQWQTLFYGKRYSNTVLQDQADFVKVAEALGV
                     KGIRVTKKEEMGPAIREAIEGGKPALIDVWIDCDEKVFPMVPAGAPIEDVFDAEDLAK
                     KEQEKD"
BASE COUNT          524 a          680 c          587 g          765 t
ORIGIN      
        1 agatgggtgg ctgcatttcc tacaagctgg ccgatggcac caaggccgat gataccgagt
       61 ttctttccgc tgatctcaca gccggcaaat ttctttttct gtttttctgc cgtttttgcg
      121 atgtcgccgt cttctttgtc actcttgacc cattcgatac cacctgcgat atcacgggat
      181 gccagcagca taccggcaag tacaagctct ttgacgccat ttgcattggc tcccggtgta
      241 ttgaagacca cgataccctg ctctgcgcag tcggtaagcg gaatgttgtt gacgccggct
      301 cctgctcttg ctacagcgag gaggctcttc ggcagctcca tgccgtgcat ggctgcactt
      361 ctgacgagaa ccgcatcagc tccctccagg ctctcgatct gctgatagtt ttcattaaag
      421 tttgcaaggc ctacgcctgc aatcggattt aagcaatgat actgaaacat tagagatttt
      481 cctcctcgaa ctttttcatg aaggctacga gtgtttctac gccttttttc ggcattgcat
      541 tgtagatgct tgcacgcata ccgccgacgg tacgatggcc tttcaggttc tcaagtccgg
      601 cttctttgga ctctttgacg aatttcgcat ccagatctgc atttccggtg ataaacggaa
      661 cgttcatcag agaacggtct tccggtacaa cggttccttt gaagagcttg ctctgatcca
      721 ggaaatcata cagcagcttt gctttctctt cgttgcgctg tttcataact tccaggccgc
      781 ccattttctt cagccatttg aagactttgc cgcacatgta gatgcagtag cagttcggtg
      841 tgttgtacag ggagcctgcg tctgcgtgtg tcttgaattt catcatggtc ggtgttcccg
      901 gcaggacatc gtcggtgatc agatcgtcac ggataatgga gatcaccatg ccggcaggtc
      961 cgatgttttt ctggacaccg ccgtagatga tgccgtaatc agagacattg acaggttcgg
     1021 agaggaaaca ggaggagacg tctgccacca gggttttgcc cttggtattc ggcagttttt
     1081 tgtatttggt accgtaaatg gtgttgtttt cgcagatata aacatagtcg gcatccgggc
     1141 tgatcggaag atcgctgcag tccgggatgt aggaaaatgt tttatcctca gaggaggcaa
     1201 ttttgtttgc ctttccgtat ttctgtgctt ctgcgtatgc tttctttgcc cactggcctg
     1261 ttacgatgta atctgcgaca ccgtttttca tcaggttcat cgggatcatg gaaaactgca
     1321 gggatgcacc gccctggaga aacagaactt tgtaatttga cgggattccc atcagatcac
     1381 gaagatctgc ttctgcctca tcgatgatgt tctggaatac tttggaacgg tggctcatct
     1441 ccatgacgga catgccgcat ccgcggtaat ccagcatttc ctcctgggct tcttttaaca
     1501 cttcctctgg cagaaccgcc ggtcctgcgg aaaaattata gactctgctc atttttctca
     1561 tcctcctgtt tctcttttct gcaccagatt actccgcact tcgtgctccc gtaatctgca
     1621 aaatattcgt actccgctca tttttctccg aaaaatggct acttactcat attttgcggg
     1681 tcccaagttc aaaaatcttc ctgtaagctt tgcttgcgtc agactttcga acttttgccc
     1741 ctggtgttac tttatcttac taggaaacca ccgatagtag accgatggtt tccattgtaa
     1801 tcacatctgt gcgattcatg cggtaatacg gtattgcttt accacgttga aactatgata
     1861 cctctttgtc agaaacctgt caatcctttt cttgttcctt ttttgccaga tcctctgcat
     1921 cgaagacatc ctcaatcgga gctcctgccg gtaccatcgg gaatactttt tcgtcgcagt
     1981 cgatccagac atcgatgagc gccggttttc cgccctcaat tgcctcacgg atggcaggac
     2041 ccatctcctc ttttttcgtc acgcggatgc cctttacgcc gagcgcctca gcaaccttga
     2101 caaagtctgc ctgatcctgg agaacggtat tggaataccg ttttccgtag aacagcgtct
     2161 gccactggcg taccatgccg agtacgtggt tgttgacaac aacctcgatg accggaatgt
     2221 tgtgtctgga aagtgttgcc agctcattca tgttcatacg gaaacagccg tcacctgcga
     2281 tgttgatgac cgttttgtcc gggcgtccca tttttgcgcc catggctgct ccaagaccga
     2341 atcccatcgt tccaagaccg ccggaggtca gaagcgttct cgggctcgta taacggtaat
     2401 actgcgccgc ccacatctga tgctggccga catccgttgt gataattgcc tgtccgtccg
     2461 ttgccttata gatctcctcg atcacatacg gtccggtaag cccctcttca tgctttaacg
     2521 ggtatttttc tttcatttcc ctgatctttg caatcc
//
LOCUS       DAOMUF010000242         2554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11625_length_2554_cov_3.273709, whole genome shotgun sequence.
ACCESSION   DAOMUF010000242 DAOMUF010000000
VERSION     DAOMUF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11625_length_2554_cov_3.273709"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLN77_10035"
     CDS             complement(<1..830)
                     /locus_tag="PLN77_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HRM44644.1"
                     /translation="MNRNRKKKNRKKETGAVVSLLLASSLAFGGCGTAVTSSSSVNAE
                     SARTESTGETSADNADTTSESTDSENAMESASDINFDLELTESTIDTEFTDREKSGSY
                     KASEAVKITLNKTTATVSGSGAKADGSTITITEEGVYIVSGTLEDGQIIVDASDSDKV
                     QIVLDGVHINCETNAAIYVREADKVFITLAENSSNTLGGGNEYTQIDDNTVDGVIFSK
                     SDLVCNGTGSLTIEADYKHGIVSKDDLVITGGTYKIAAADNGITAKDQIKILNGSFDI
                     "
     gene            complement(830..1283)
                     /locus_tag="PLN77_10040"
                     /pseudo
     CDS             complement(830..1283)
                     /locus_tag="PLN77_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1571..2344)
                     /locus_tag="PLN77_10045"
     CDS             complement(1571..2344)
                     /locus_tag="PLN77_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRM44645.1"
                     /translation="MMQSQQIPMTFQRYETKYMLSPIMARKLRKSLPGHLQMDEYGLD
                     TICSLYYDTPDHQLIRRSLEKPVYKEKLRLRSYGPAKETSPIYVELKKKYEGIVYKRR
                     ERMILSEAVPYLAGKSEPGFDSQILHEITWFRNYYRGLVPAMYIAYDRIACFGTEDRN
                     FRVTFDTNIRWRTDNLCLDGPTEGTRLLEPGWYLMEVKTPGAVPLWFSKILDDLAIYP
                     ASFSKYGFAYQIENQKASEEKAFIPFTDIRQTVSKTAFR"
BASE COUNT          586 a          573 c          615 g          780 t
ORIGIN      
        1 tcaatgtcaa agcttccgtt cagaatttta atctgatctt tggctgtgat cccgttgtcc
       61 gcagcggcga ttttgtaggt tccgccggta atcacaaggt catctttgga tacaatcccg
      121 tgtttgtagt ctgcctcgat ggtcagactt cctgtcccgt tgcagacaag atctgatttg
      181 gagaagataa cgccgtctac cgtgttgtcg tcaatctgag tgtactcgtt tccaccgcca
      241 agcgtgttgc tgctgttttc agcaagcgtg atgaaaactt tgtccgcttc gcgaacgtag
      301 atggcagcat ttgtctcaca gttgatatgg actccatcga gtacgatctg aaccttgtcg
      361 gaatcggatg cgtctacgat aatctggcca tcctcaagtg ttccgcttac gatgtaaacg
      421 ccttcttccg tgatggtaat tgtgctgccg tctgctttgg ctccgctgcc ggagacggta
      481 gctgtcgtct tattaagtgt aatttttacg gcttccgacg ctttgtagct tccgctcttc
      541 tcacggtctg tgaattcggt gtcgatagtg gattcggtca gttccagatc aaaattaata
      601 tcggaagcac tttccattgc attttccgaa tctgtgctct ctgatgtggt atccgcgttg
      661 tcggcagatg tttcgccggt tgactctgta cgggcagatt cggcatttac agagctggaa
      721 gatgtcactg ctgtgccgca tccgccgaag gccagcgatg atgcaagaag caggctgacg
      781 actgcgccgg tctctttttt tctgtttttt ttctttctgt ttcgattcat tataattcct
      841 cccttccggt ctgcgcacga ctgcacagga tttcaaggtt tccgtttctg cagcggagct
      901 ggtcaatcat gtttttctct tcgccttcct cgcggaatac gatgcgatac tccagcttga
      961 aaagacttcc catattcgtg gtttttacct gtacaagctc gcaacgggtc agatacctgg
     1021 caaagatatc gtcaaaaaca ctggtataat caagggactc cggttccaag atttccattg
     1081 acgagcatga tcaccatctg tacgatcaca ggcatcagaa caagcgtgac agcaaagctt
     1141 ttggtgcatt cgcttttgta cctgtaaaca gcagcgatca acagtccctg aatcagagac
     1201 actgctgtgc aggcaaagaa ctgaccaaag gtgaaggcgg cggttgtttc tgtaaaaacg
     1261 gaactgaata ttgtgttaag catagcaaat tcctcccaga atttttttat tttcttgtct
     1321 attcagagcc ccatctcgat tccgcttacg ctacatcgcg atgttgcttc tcgccatatg
     1381 aagttccaga aaaaccgctc attcatgcaa gcatgattcg cgatttttct tctattttgc
     1441 atggctctga atagttacat tttctttttt tgtgaagcag actatgtttg aaaaaggctt
     1501 ttcgcaagct gtgagtccac ccactgcttc ttgcttttcg caatggccgc agaggactta
     1561 cctgattcag ctaacggaat gctgtttttg atacggtttg ccggatatct gtgaatggta
     1621 tgaaagcttt ttcttctgat gctttctgat tttcgatctg ataggcaaag ccgtatttag
     1681 aaaatgatgc cggatagatt gcaagatcat ccagaatttt gctgaaccat aggggcacag
     1741 cacctggtgt tttcacctcc atgagatacc agcctggttc taaaagcctg gttccttccg
     1801 ttggaccgtc aagacacaga ttgtcggtac gccatcggat attggtgtcg aaggtaacac
     1861 gaaagttcct gtcttccgtg ccgaagcagg cgatgcggtc gtaggcgatg tacatagcgg
     1921 gaacaagccc ccggtagtag ttgcgaaacc aagtgatttc gtggagaatc tggctgtcga
     1981 agcccggttc tgattttccc gcaagatagg gaaccgcttc tgataatatc atgcgctcgc
     2041 ggcgtttgta gacgattcct tcatattttt tctttagttc tacgtatatg gggctggtct
     2101 ctttggccgg accataactg cggagtcgca gtttttcttt gtagacgggt ttttccagcg
     2161 aacggcggat gagctggtga tccggggtat cgtaatagag gctgcagatg gtatcaaggc
     2221 cgtattcatc catctgcaga tgacccggaa ggctcttgcg aagcttgcga gccatgatcg
     2281 gggaaagcat gtattttgtt tcataccgtt ggaaggtcat gggaatctgt tggctttgca
     2341 tcataatcga ttgatccttt cgtttgattt acttccggaa atgtttttta catttttaag
     2401 tataatgagg aaactttaaa ggaaccttaa agaatggacg gaaaatgtga aaggtttgtg
     2461 aaaagacgga cctctgtaat ccggatcgct ttttgaatac catataaaaa aagaaagagc
     2521 cgtttccgga caaattcgtt ggaagcggat ttgt
//
LOCUS       DAOMUF010000243         2551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11648_length_2551_cov_2.604167, whole genome shotgun sequence.
ACCESSION   DAOMUF010000243 DAOMUF010000000
VERSION     DAOMUF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11648_length_2551_cov_2.604167"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLN77_10050"
     CDS             <1..185
                     /locus_tag="PLN77_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44646.1"
                     /translation="SLLVLRFFFYYKNSGFLCQEIDFYYFQGKNIIFFKKLLDKPKNE
                     TIMQSVQKKKGIRKKQ"
     gene            586..2283
                     /locus_tag="PLN77_10055"
     CDS             586..2283
                     /locus_tag="PLN77_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRM44647.1"
                     /translation="MNTLVIVLIAAVCLIAGYTFYGSWLAKKWGIDPKAKTPAVAKND
                     GQDYVPTDGWTVFAHQFSSIAGAGPVTGAIQAAVFGWVPVLLWILIGGIFFGAVTDFG
                     ALYASVKNEGKSMGLLIEKYIGKTGKKLFLLFCWLFTLIVIAAFADMVAGTFNAYETV
                     DGVTKLSAQATVNGAAGSISLLFIAFAVVFGLLQKKLQWKGWKEMVLGLACTVAAFAI
                     GMNVPLVTSKATWTYLVFAYIFLAAVLPMWLLMQPRDFMTTFMFAGMILGAVVGLVVA
                     HPKMNLPMYTGFHNAASGDLFPILFVTVACGAVSGFHSLVSSGTSSKTIANEKDMQKV
                     GYGAMVLESLLAVLALCVAGAAASADGTAAVGTPFAIFSSGVAGFLEMFGIPVYGAQC
                     FMTMCVSALALTSLDSVARIGRMSFQELFTVDDMEHAAGWRKLLCNKYFSTIVTLLCG
                     YILTQIGYANIWPLFGSANQMLSALVLITLCVFLKVTGRENRTLFPPLIIMLCVTFTA
                     LVERVIALVKAYGAGTAVFLVEGLQLIIAILLMVLGVIIVISCGKELFRKKAGQETK"
     gene            complement(2380..>2551)
                     /locus_tag="PLN77_10060"
     CDS             complement(2380..>2551)
                     /locus_tag="PLN77_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM44648.1"
                     /translation="IGATKTAYLEDAVKAFAVTLTDDDIAYLEEPYIPHRIVGAVDHN
                     LADGVMLLDEKK"
BASE COUNT          624 a          605 c          605 g          717 t
ORIGIN      
        1 gaagccttct ggttctgcgc tttttcttct attataaaaa ttctggtttt ctgtgtcaag
       61 aaatagattt ttactatttt cagggaaaaa acataatatt ttttaaaaaa ttgcttgaca
      121 aaccgaaaaa tgagactata atgcaaagtg ttcaaaagaa aaaagggatc agaaaaaagc
      181 aatgagcagg acaacatttt tcgggtgaac ctttgcagag aaccatcggc tggtgcgaga
      241 tggcaggtat cctaaagaac aatcccctgt gagctgtaca gacgaaacca gaaatggaaa
      301 gtagtccgga ccggcctctc accgttagca gagaagagat tcaccggaaa gtgtatcaaa
      361 tggtttcggg gatcttgtct gagtgatcga ccaaaagaaa cagccgataa ttagaagtgg
      421 taacgcggag cgttttttga aagcttcgtc ttcttgcata agtcagggag acgaagcttt
      481 ttttgtttcc aagcatacca acacaattgc cttttccctt ttctttttcc atttggaatc
      541 catgtaaact ggaaatatca tttttgaacg aggaggaatt ttattatgaa cacgttggtt
      601 attgtactga tcgccgccgt atgtctgata gcaggttata ccttctacgg aagctggctg
      661 gcaaagaaat ggggcatcga tcccaaagca aaaacacctg ccgttgcaaa aaatgacggt
      721 caggattacg tccctaccga tggatggacc gtattcgccc atcagttttc ttcaatcgca
      781 ggtgcaggcc ctgtcaccgg tgccattcag gccgctgtct ttggatgggt tcccgtcctt
      841 ttatggatcc tgatcggcgg catcttcttt ggcgctgtta ctgatttcgg cgcgctgtat
      901 gcgagtgtca aaaatgaagg aaaatccatg ggacttctca ttgagaaata catcggcaaa
      961 acaggaaaga aactgtttct tctcttctgc tggctcttta cactgatcgt tatcgccgcc
     1021 tttgcagata tggttgccgg aacatttaat gcttacgaaa cggttgacgg cgtcacaaag
     1081 ctttctgccc aggcaactgt aaacggcgct gccggaagca tctccctgct gtttatcgct
     1141 tttgctgttg tcttcggact gctccagaaa aaactgcagt ggaaaggctg gaaagaaatg
     1201 gtactcggcc ttgcctgcac tgttgcagcc tttgcaatcg gcatgaatgt accgcttgta
     1261 acaagcaaag caacctggac ttatctggta tttgcctaca tctttcttgc cgctgtcctt
     1321 ccgatgtggc ttctgatgca gccgcgtgac tttatgacaa cctttatgtt tgccggcatg
     1381 atccttggcg ctgttgtcgg acttgttgtt gcgcacccga agatgaacct tccaatgtat
     1441 accggattcc ataatgcggc atccggagat ctgttcccga tcctgtttgt caccgttgcc
     1501 tgtggtgctg tttccggatt ccacagcctg gtatcgtctg gaacctcttc gaaaaccatt
     1561 gcgaatgaaa aagatatgca gaaagtcgga tacggagcta tggttctgga gagcctgctt
     1621 gccgtcctcg ccctctgcgt tgccggtgct gctgcttctg cagacggtac ggctgctgtc
     1681 ggaacgccat ttgcgatctt ttcttccggt gttgccggat ttctggaaat gttcggcatc
     1741 ccggtatacg gagcacagtg cttcatgacg atgtgtgtat cggcgctggc acttaccagt
     1801 cttgactccg ttgcacgaat cggccgtatg tccttccagg aactgttcac tgtcgatgat
     1861 atggaacatg ctgccggatg gagaaagctg ctctgcaata aatatttctc taccattgtt
     1921 actctgctct gcggctacat cctgacccag atcggatatg ccaatatctg gccgctgttc
     1981 ggaagtgcaa accagatgct gagtgcactc gttctgatca cgctctgtgt cttcctgaaa
     2041 gtcaccggcc gtgaaaacag aaccctgttc ccgccgctta tcatcatgct ctgcgtcacc
     2101 tttaccgcac tggtagagcg tgtcatcgca cttgtaaaag cgtatggagc aggaaccgct
     2161 gtcttcctcg ttgagggact gcagctcatc atcgccattc tgctgatggt tcttggtgtc
     2221 atcattgtca tcagctgtgg aaaagaactg ttccggaaga aagcgggaca ggaaacgaaa
     2281 taatatagat gtttcttcaa agagcttcct aacgtaaaac gccgtcagac tacaacaagt
     2341 ctgacggcgt tttacatgcc atagtttttt acgaaacaat tatttctttt catccagcag
     2401 cataacccca tctgccagat tgtgatcaac ggcaccaacg attcggtgcg gtatatacgg
     2461 ttcttcgaga tacgcgatgt cgtcatcggt taatgttaca gcgaaggctt ttaccgcatc
     2521 ttccagatac gccgtcttgg tcgcaccgat a
//
LOCUS       DAOMUF010000244         2534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11748_length_2534_cov_2.758370, whole genome shotgun sequence.
ACCESSION   DAOMUF010000244 DAOMUF010000000
VERSION     DAOMUF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11748_length_2534_cov_2.758370"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLN77_10065"
     CDS             <1..374
                     /locus_tag="PLN77_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM44649.1"
                     /translation="GDVVDSMVAFLSGFVLYFYLLYLLRGRMSKIATNISGGALVTLI
                     AVFLYQAGIGHHLDKVIIGSIIPLVPGVNFTNAIRDIADQDYIAGSVRMLDALLVTFC
                     IALGVGLIITGYHQLAGGLQL"
     gene            371..826
                     /locus_tag="PLN77_10070"
     CDS             371..826
                     /locus_tag="PLN77_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM44650.1"
                     /translation="MSDTIRLIAEFFAACVGTIAFALLFQVPRKYYLYCGLAGACGWI
                     CYKLILPHTTEPVSIFFATVLVILLSRSFAVYKRCPVTVFLIAGIFPLVPGSGIYWTA
                     FYVVTNDLERAGARGFLTLKDAVAIVLGILFVFEFPQSWFRKVLTDKNK"
     gene            872..1651
                     /locus_tag="PLN77_10075"
     CDS             872..1651
                     /locus_tag="PLN77_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRM44651.1"
                     /translation="MALNQFSRTQLLLGQESMDLLANATVAVFGIGGVGGYTVEALAR
                     SGVGHFVLVDDDKVCLTNINRQIIATRSSVGKYKVDVMKERILDINPKAEVEVYKCFY
                     LPENADDFDFSKYSYVVDAVDTVTAKIEIIMRAKACNVPVISCMGAGNKLDPTKFRVA
                     DIYKTNMCPLAKVMRREMKKRGVKKLKVVYSEEKSLRPLEDMSISCRTNCICPPGAAR
                     KCTERRDIPGSVAFVPSVAGLILAGEVIKDLSGVRERRQLQ"
     gene            complement(1741..>2534)
                     /locus_tag="PLN77_10080"
     CDS             complement(1741..>2534)
                     /locus_tag="PLN77_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44652.1"
                     /translation="VYQAVLDAGWDGEWFLRAYDAAGEKVGSNECEEGKIYIEPQGFC
                     VLAEIGVKEGLAEKALTSVQNILETKYGVVLLQPAYTKYYLNLGEVSSYPPGYKENAG
                     IFCHNNPWISIAETVVGHGNRAFDLYRKICPAYIEDISEIHRTEPYVYSQMIAGKDAA
                     HFGEAKNSWLTGTAAWTFVNVSQYILGIQPDYDGLTLNPCIPSDMEEFKIRRYFRGAW
                     YNITFQNPEHREKGISSLTVNGTAVEGNLIPLTEGCTEYDVVAVM"
BASE COUNT          596 a          577 c          711 g          650 t
ORIGIN      
        1 gcggcgatgt ggtggacagc atggtagcgt ttctgtcggg ctttgttctg tatttctatc
       61 tgctgtacct gctgcgtggc agaatgtcaa agattgcgac gaatatttcc ggcggcgcgc
      121 ttgtcacgct gattgccgtt tttctgtatc aggcggggat cggccatcat ctggacaagg
      181 tcatcatcgg ttcgattatt ccgctggtgc ccggtgtcaa tttcacgaac gccatccgtg
      241 atattgcgga tcaggactac atcgctggtt ccgtgcggat gctcgatgcg ctgcttgtga
      301 cattctgtat tgcgcttggc gtcggactga ttatcacagg atatcatcaa ctggcgggag
      361 ggctgcagct atgagtgata ccattcgttt aattgcggaa ttttttgccg cctgcgtcgg
      421 aacgattgcg tttgcgctgc tgttccaggt gccgcggaaa tactacctgt actgcggact
      481 ggcaggtgcc tgcggctgga tctgctataa actgatcctc ccgcatacga cagagccggt
      541 gtccatcttt tttgcaacgg tgctcgtcat ccttctgtcg cgcagctttg cggtgtacaa
      601 gcgctgcccg gtaacggtat ttctgattgc gggtattttc ccgctggtgc cgggaagcgg
      661 catctactgg actgcttttt acgtggtaac caatgatctg gagcgcgccg gtgcaagagg
      721 atttctgaca ctgaaggatg cagttgcgat tgtgcttggc attctctttg tttttgagtt
      781 tccgcagagc tggttccgta aggtgttgac agacaaaaac aaataaccta ttattgtagt
      841 agagattata aacaaggagg aaggaaaaca aatggcatta aatcagtttt ccagaacaca
      901 gcttctgctc ggacaggaga gcatggatct tctggcgaat gcaaccgttg ccgttttcgg
      961 tatcggcggc gtaggcggat ataccgtaga ggcgctggca agaagcggtg tcggccattt
     1021 tgtgctggta gacgatgata aggtctgcct caccaacatc aaccgtcaga ttattgcgac
     1081 gagaagcagt gtcggaaaat ataaagtaga cgtaatgaaa gaacgcattc tggatatcaa
     1141 tccgaaagcg gaggtagagg tatacaaatg cttttatctg ccggaaaatg cggacgattt
     1201 tgatttttcg aagtattcat acgtggtgga tgctgttgat accgtgacag cgaagattga
     1261 gattatcatg cgcgccaaag cgtgcaatgt gccggtcatc agctgcatgg gagctggaaa
     1321 caagctggac ccgacgaagt tccgcgtggc ggatatctat aaaacgaaca tgtgtccgct
     1381 ggcaaaggtc atgcggcgtg agatgaaaaa acgcggcgtg aagaaactga aagtggtcta
     1441 ttcagaggaa aaatccctcc gtccgcttga ggatatgagc atcagctgcc ggacaaattg
     1501 tatttgtcct ccgggagctg caagaaaatg cacggagcgg cgtgatatcc cgggcagcgt
     1561 agcgtttgtg ccgtccgtag caggactgat tctggcggga gaggtcatca aagatctttc
     1621 cggtgtcaga gagagacggc agctgcagta aaaaataaga ataaaaggga cgagccccgg
     1681 aaaagcagtt gtgctttccg gggctcgcct tgtgtttatg gagatttgaa atttgaaaaa
     1741 ttacattacg gcaacaacat catattcggt gcagccttcg gtgagcggaa tcagatttcc
     1801 ttctactgcg gttccgttta ccgtgaggga agaaatacct ttctccctgt gctccggatt
     1861 ctggaaggtg atgttatacc atgcaccgcg gaagtatctg cggatcttga actcttccat
     1921 atcggacgga atacacggat ttaaggtcag tccgtcgtaa tccggctgaa tgccgaggat
     1981 gtactgggaa acattgacaa aagtccatgc ggcagtaccg gtcagccagg agttctttgc
     2041 ctcaccgaag tgagcagcgt cttttccggc gatcatctga gagtagacgt acggctcggt
     2101 gcggtggatc tcgctgatgt cctcaatgta agccgggcag atcttgcggt acaggtcaaa
     2161 tgcgcggttt ccgtggccga caacggtctc agcgatggag atccatgggt tgttgtggca
     2221 gaagatacca gcattttctt tgtatcccgg cggataggag gaaacctcac caagattcag
     2281 gtaatacttg gtgtaagccg gctgcagaag aacaacgccg tacttggttt ccagaatgtt
     2341 ctggacagaa gtcagggctt tttctgccag tccttctttg acaccgattt cagcaagaac
     2401 acagaagccc tgcggctcga tgtagatctt accttcctca cactcattgg agccgacttt
     2461 ttcgcctgct gcatcataag cacgcaggaa ccattcgccg tcccagcctg catcaaggac
     2521 tgcctggtaa actt
//
LOCUS       DAOMUF010000245         2533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11754_length_2533_cov_3.412429, whole genome shotgun sequence.
ACCESSION   DAOMUF010000245 DAOMUF010000000
VERSION     DAOMUF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11754_length_2533_cov_3.412429"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2192)
                     /locus_tag="PLN77_10085"
     CDS             complement(<1..2192)
                     /locus_tag="PLN77_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRM44653.1"
                     /translation="MATFYASKTGEVSAREKEHSALVRELAGECMTLLENDGTLPLAG
                     AGKVAVYGNGVRHTVKGGTGSGDVNTRTVVTIEQGLKEAGFEILTGKWLDEYDKVLAD
                     AQAAYQAELAKKAEELHVPIFAVMFSEVFAQPDVPVITEKEDTDTAIYVLSRNSGEGA
                     DRYNRACDYLLGENELADIAYLAEHYEKTVLILNIANLVDTTELKKIKGLNAILLAGQ
                     AGNATGNIVADVVLGKSIPSGKLTDTWAASYEDYPSSENFSHNNGDTNDEYYSDGIYV
                     GYRYFDTFNVTPNYCFGYGKGYTDFETEVRDVKADDKNVTVTASVKNIGDTFAGKEVV
                     QVYYSAPDGTIEKPYQELGGFGKSDLLSPGESQTITISFPTRSMASYDEKKAAWVLEA
                     GTYYIRVGNSSRTTKVAAALNLKETVVTVQGKNLFPADDAPQELSKAGVTPYSYEGEA
                     EEKAAAKQIEICSKCIKTETVIYSGTPEAFPVYEGEKLTAADVKSGKATLKDLVSQLT
                     VEEMATVCNGTADGLGQEGFIGSSSDMAPGAAGDTTSILLADRGIYNTILADGPAGLR
                     LIPHFVVDADGKMVSSGNPLEDAFNKNEIEVPEGGTEYFQYCTAIPVAALLAQSWNMD
                     LIQKCGDIVGKEMEEFHISVWLAPGMNIHRNPLNGRNFEYISEDPILTGRICAAQVKA
                     MAKSGIGSTIKHFCGNNQEVGRSTSDSVISERALREIYLKGFEIAVKEG"
BASE COUNT          580 a          644 c          513 g          796 t
ORIGIN      
        1 ccctctttta ccgcaatctc aaaacctttc agataaatct cacgcaaagc tctctcagag
       61 atcacagagt ccgaagtact tcttccaacc tcctgattat taccacagaa atgcttgatc
      121 gtacttccga ttccagactt cgccatagcc ttcacctgag ctgcacaaat cctgcctgta
      181 agaatcggat cctcagaaat atactcaaag ttacgtccat tcaacggatt tctgtgaatg
      241 ttcatgccag gtgccagcca tacggaaatg tggaattctt ccatctcttt tccgacgata
      301 tcaccgcatt tctgaatcag atccatgttc cacgattgtg caagaagcgc tgctaccggg
      361 attgcggtgc agtactggaa atactccgtg ccgccttccg gaacttcgat ttcatttttg
      421 ttaaacgcat cttccagagg atttccggag gataccatct ttccgtctgc atcaacgaca
      481 aagtgcggaa tcagacgaag accggccgga ccgtctgcaa gaatcgtatt gtaaatacct
      541 ctgtccgcaa gaagaatgga agtcgtatct cccgctgctc caggcgccat atcggaagaa
      601 gatccgataa agccttcctg tccgaggccg tctgccgtac cattgcaaac ggtagccatc
      661 tcttctacgg tcagctgcga tacgagatct tttaacgtcg cttttccgct ctttacatct
      721 gccgcagtca gcttctctcc ctcataaact gggaatgctt ccggtgttcc actgtaaata
      781 acggtctctg tcttgatgca tttactgcaa atctcaatct gttttgcagc tgctttttct
      841 tctgcctcac cttcatagct gtacggtgtt acgcctgctt tggagagttc ctgcggagcg
      901 tcatctgccg ggaagagatt ctttccctga accgtaacaa cggtctcttt gagattcagg
      961 gctgctgcca ctttcgttgt tctggagctg ttaccgacac ggatgtaata cgttcctgct
     1021 tcgagtaccc atgctgcttt tttctcatcg taggaagcca tagatctcgt cgggaaggaa
     1081 atggtaatcg tctgggactc acccggcgag agcagatcgg attttccgaa gccgccaagc
     1141 tcctgatacg gtttctcaat cgttccatcc ggtgcggaat agtaaacctg tacgacttct
     1201 tttccggcga atgtatcacc aatattcttt acggatgcgg taacggtaac gttcttatca
     1261 tctgccttca cgtcccttac ttccgtctcg aaatcagtat agcctttgcc gtagccgaag
     1321 cagtagttcg gggttacgtt gaaggtgtca aaataacggt agccgacata aatgccatcg
     1381 ctgtaatact catcgttagt gtcaccgttg ttgtggctga aattttcgga ggacggatag
     1441 tcttcatagg aagcagccca tgtatcggtc agctttcctg atgggatgct ctttccgaga
     1501 acaacgtctg caacgatgtt tccggttgca tttccggcct gtcctgcaag aagaattgca
     1561 ttcagtcctt tgattttctt taactctgtg gtgtctacaa ggttcgcaat attcaaaatt
     1621 aaaactgttt tctcataatg ctctgccaga taggcaatgt cagcgagctc gttttcgccg
     1681 agcagataat cgcatgctct gttatagcgg tctgcacctt caccggagtt tcttgacaga
     1741 acgtagatag cggtatcggt gtcctctttt tcggtaatta ccggcacgtc aggctgcgca
     1801 aatacttccg agaacataac agcaaaaatc ggaacgtgaa gctcctctgc tttttttgcc
     1861 agctctgcct gatatgcagc ctgcgcatct gcaagcactt tgtcatattc gtcaagccat
     1921 ttaccggtca gaatttcaaa gccggcctct tttaagccct gttcgatcgt aacaaccgtt
     1981 cttgtgttaa cgtctccgga accggtaccc ccttttaccg tgtggcggac gccgttacca
     2041 taaactgcga cttttcctgc accggcaagc ggcagggttc cgtcgttttc aagaagtgtc
     2101 atgcactcac cggccagttc acgtaccagc gcggaatgtt ctttttctct agcagatact
     2161 tctcctgttt ttgatgcata aaatgttgcc ataattctaa tacctcccaa taacaaattc
     2221 acatatagcg cttttattgt aacgcatttt tcgtcattat gcaaaagttt tttcacccgt
     2281 tttctcatat tttatttgta ttttgtgcat tttctcttat tatccgactt ttctgtcttt
     2341 ttttcgctca ttgatttctt tttttatttt ttatattgat gcttttattc tattgatttt
     2401 tctgtaaacg atacgctttt tccacctttt ttcgtcttct ttcacaaaat tcgttgacgc
     2461 gtttgatttt ggcagtataa tggaatcaca gatcaatttt aggaggcccc aattatgtta
     2521 aaacaacttt cta
//
LOCUS       DAOMUF010000246         2531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11776_length_2531_cov_2.996769, whole genome shotgun sequence.
ACCESSION   DAOMUF010000246 DAOMUF010000000
VERSION     DAOMUF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11776_length_2531_cov_2.996769"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1102
                     /locus_tag="PLN77_10090"
     CDS             77..1102
                     /locus_tag="PLN77_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM44654.1"
                     /translation="MSMVTLGSTGICVNKNGFGALPVQRVTKEDAVYLLRKAYDGGIR
                     FFDTARAYSDSEEKLGAAFEGMREKVFISTKTMAKDVEGFWKDLETSLSLLKTDYIDI
                     YQFHNPSFCPKPGDGTGLYEAMLEAKAQGKIRHIGITNHRMSVAEEIIESGLYETLQF
                     PFCYLATDREKALVQGCKEKNIGFIAMKALSGGLITNSAAAYAFEDQYDNVLPIWGVQ
                     REKELDEFLSYIACPPAMTDEIKTIIEKDQKELSGNFCRGCGYCMPCPAGIEINTCAR
                     MSLLLRRSPSANQLTERGQAMMKKIEGCLHCGACMKKCPYGLNTPKLLEENYEDYKNV
                     LAGKTLV"
     gene            1099..2043
                     /locus_tag="PLN77_10095"
     CDS             1099..2043
                     /locus_tag="PLN77_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM44655.1"
                     /translation="MSNLIFSLNATMPIFLTMILGLFFRKVGILDESFTSKMNKFVFK
                     IALPVLLFQDLSDSDFSAVWDIKFVLFCFFATLLSILAVWGLSHLLKERSARGEFIQA
                     AYRSSAAILGIAFIQNIYGNSGMAPLMIISSVPLYNIMAVVVLAVFGPEREEITADFV
                     KKTAKNIVTNPIILGIAAGLLWSVLRIPKPVILQKTVQNIAVLATPLGLIAMGASFEA
                     KSAAKNIRPSIAAAAIKLVGLAAAFLPIAVILGFRQEKLVAILVMLGSATTVSSFIMA
                     KNMGHEGSLTANTVMLTTCGSAFTLTFWLYLMKTLAFI"
     gene            2151..>2531
                     /locus_tag="PLN77_10100"
     CDS             2151..>2531
                     /locus_tag="PLN77_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HRM44656.1"
                     /translation="MDFKNAVKALQIGKKEGKKFRLSTEFGKNPEEELPFPEYPRPQM
                     RRENWTNLNGWWEYAFTETQKMPKQRDGKILVPFSPECDASGVKRQLLPKEYLWYFRT
                     IQLPKIPAGKRLLLQFGAVDQDAVI"
BASE COUNT          665 a          587 c          689 g          590 t
ORIGIN      
        1 attttcgaca tgtcagactc cgtgaaatac cgactatgag ttgaaacaat gaaagcgaaa
       61 aaggaggaac acaagtatgt ccatggtaac actgggaagt acaggaatct gtgtaaacaa
      121 gaacggcttc ggcgcgctgc cggtacagcg tgtcacgaaa gaagacgctg tttatctgct
      181 gcgcaaagcg tatgacggag gcatccgctt ttttgatacc gcgcgtgcat acagcgacag
      241 cgaggaaaaa ttaggcgcag cgtttgaggg aatgcgtgag aaggtcttta tctctacgaa
      301 gacgatggca aaggatgtgg agggcttctg gaaggatctg gagacaagcc tttccctttt
      361 gaagacggat tacatcgata tctatcagtt ccacaatccg tccttctgtc ctaagccggg
      421 agatggaacc ggcctgtatg aggcgatgct ggaagcaaaa gcacagggaa aaatccgcca
      481 catcggtatc acgaaccacc gcatgagcgt ggcagaggag atcatcgagt ccggtcttta
      541 cgagacgctg cagttcccgt tctgctatct ggcaacggac cgtgagaaag cactggtaca
      601 gggatgcaaa gagaaaaata tcggctttat cgcaatgaaa gcgctctcgg gcgggctgat
      661 cacaaactcc gcggctgcat atgcttttga agaccagtac gacaacgtgc ttccgatctg
      721 gggcgtccag agagaaaaag agctggacga gttcttaagc tacattgcct gtccgcctgc
      781 catgacggat gaaatcaaga cgatcatcga gaaagaccag aaagagctct ccggcaactt
      841 ctgccgcggc tgcggttact gtatgccatg tccggcaggc attgagatta acacctgcgc
      901 gcgcatgagc ctgcttctgc gccgttcccc gtccgccaac cagctcaccg agcgcggcca
      961 ggcgatgatg aagaaaatcg aaggttgtct ccactgcggc gcctgcatga agaaatgtcc
     1021 ctacggtctg aatacgccga agcttctcga agagaactac gaagattata aaaatgtact
     1081 ggcaggaaaa acactggtat gagtaatctg atcttcagcc tgaacgcgac gatgccgatc
     1141 ttcctgacaa tgattttggg acttttcttt cgaaaggttg gtattctgga cgaatcgttc
     1201 acaagcaaaa tgaacaaatt tgtgtttaaa atcgcactcc cggtgctgct gttccaggac
     1261 ctgtcggatt ctgatttctc cgctgtgtgg gatatcaaat tcgttctgtt ctgcttcttt
     1321 gcaacactgc tctcaattct tgcggtatgg ggactttccc atctcttaaa agagagaagt
     1381 gcaagaggcg aatttatcca ggcggcgtac cggagcagtg cggcgatcct tggtattgcc
     1441 tttatccaga atatctacgg aaattccggc atggcgccgc tgatgatcat cagcagtgtg
     1501 ccgctgtata atatcatggc ggttgtcgtt cttgcggtat tcggtccgga acgggaggag
     1561 attacggcag attttgtgaa aaagacggcg aaaaatatcg tgacaaaccc gattatcctc
     1621 gggatcgctg caggacttct ctggtcggtg cttcgcattc caaagccggt gattctgcag
     1681 aaaacggtgc agaatattgc ggtacttgca acgccgctcg gcctgattgc gatgggtgcg
     1741 tcgtttgagg caaagagcgc ggcaaagaat atccggccgt cgattgctgc agctgcgatt
     1801 aagctggtcg gtcttgcagc tgcctttctg ccgatcgccg tgatacttgg cttccgtcag
     1861 gaaaaattgg tggcaatcct tgtgatgctg ggttccgcaa cgacggtgag cagttttatc
     1921 atggcgaaaa atatgggtca tgagggaagt ctgacggcaa acaccgttat gctcacaacg
     1981 tgcggcagtg cctttacgct gacgttctgg ctgtatctga tgaaaacgct ggcgtttatc
     2041 taaccggatt gtgaatatcc gctttaattt tcagtacacc aaaagcgtgt ccgggatttt
     2101 gtacgggcac gcttttatca atcataaaaa tcagaaaaag agggaaaaga atggacttca
     2161 aaaatgcggt caaagcatta cagattggaa aaaaggaagg aaaaaaattc cgcctttcta
     2221 cggaatttgg gaaaaatccg gaagaagagc tcccgtttcc ggaatatccg cgtccgcaga
     2281 tgcgccggga aaactggaca aatctgaacg gatggtggga gtatgcgttc acggaaacgc
     2341 agaaaatgcc aaaacagcgg gacggaaaaa ttctcgttcc gttttccccg gagtgcgatg
     2401 cgtccggcgt gaagcggcag ctgcttccaa aagaatatct gtggtacttc cgcacgattc
     2461 agcttcccaa aattcctgcc ggaaaacggc tgctgctgca gttcggcgcc gtggatcagg
     2521 atgcggtaat t
//
LOCUS       DAOMUF010000247         2505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_11935_length_2505_cov_3.393061, whole genome shotgun sequence.
ACCESSION   DAOMUF010000247 DAOMUF010000000
VERSION     DAOMUF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11935_length_2505_cov_3.393061"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLN77_10105"
     CDS             <1..370
                     /locus_tag="PLN77_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HRM44657.1"
                     /translation="TRVGAGCFFLMGTLKIFEELRKRRRKKQGKPEARLEHLEKQLHP
                     LEFGLLAVSMSVDGIVSGVFAADLPVTGGQIFFASFAVGMAAMYAGTAIGKKTGQKNG
                     GNGALFGGILFYLLAFLKLT"
     gene            510..1274
                     /locus_tag="PLN77_10110"
     CDS             510..1274
                     /locus_tag="PLN77_10110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRM44658.1"
                     /translation="MISVVIPSYNGAETLFQAIDSVLCQTTKTPLEILVIDDNSTDDT
                     LEKMGRYGENDRVRYIRNETNKGASASRNRGVQLAKGDYIAFLDADDYWDPEKLEKQE
                     KALAKTGDVLCCTGRELIYDQEPEKQKIFSVPERITYKMLLRGNVIGCSSVLAKRSVL
                     LEFPMEKDECHEDYLDWLRILKKYGTACGVDEPLLKYRLSSTGKSGNKLHSAGMTYRV
                     YRELGMGTFRSLYCFACYAVNGVRKYYGKGKKRGRD"
     gene            1274..>2505
                     /locus_tag="PLN77_10115"
     CDS             1274..>2505
                     /locus_tag="PLN77_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRM44659.1"
                     /translation="MNYKNREQYKHLMRVLAVAAIVLLECAVFSVFWDRYYASHLWLE
                     PFWDKGNQIIVVIYGIFLLVFMYIYGGMKIGYMKGSSIIYSQMMTAFCANALMYLQII
                     LLWRHLPYILPMVGMTLVDFALAGIVSWLFEKTFARLFPPREVLLIYGEYPMESLELK
                     MNQRPDKYIVAHKVSVEVGEKELCRQIDAYGQEGVILGDLHSELRNRLLKYCYAKEIR
                     VYLTPKLSDIMVRKAEALHIFDTPLLLARNSGLSFDQRFCKRLLDLLVSTILLVITSP
                     IMLIAALCIKLYDHGPVFFKQKRLTIGGKEFYVYKFRSMIVDAEKDGVARLATKNDSR
                     ITPVGKVIRATRIDELPQLLNIFKGDMSVVGPRPERPEIAKEYEESIPEFAYRLKVKA
                     GLTGYAQIYGKYNTTAYD"
BASE COUNT          670 a          558 c          709 g          568 t
ORIGIN      
        1 aaccagagtg ggtgcgggct gcttttttct tatgggaacg ctgaaaattt ttgaggaact
       61 gagaaaacgc agaagaaaaa agcagggaaa accagaagca agactggaac atctggaaaa
      121 acagctccac ccactggaat ttggcctgct ggcggtttcg atgtcggtgg atggaattgt
      181 ctccggcgtc tttgccgcgg atcttccggt gacaggagga cagatctttt ttgcctcctt
      241 cgcggtcgga atggcggcga tgtatgccgg aactgccatc ggaaaaaaga cggggcagaa
      301 aaacggcgga aacggagcac tgttcggagg aattctgttc tatttactgg cgtttttaaa
      361 gctcacatag aattacatgt aagaaagaaa aaccagcaga tcccggcgta aaattgacaa
      421 gcccgtgcag atatgctata aaagtaggag gtttgctgct ggaaaattta aaaaacagat
      481 gcagcagaca aagtaagaga ggaaaataca tgatttcagt agtaatccct tcgtacaacg
      541 gtgcagaaac actgtttcag gcaatcgact cagtcctttg ccagacaaca aaaaccccgc
      601 tggaaatcct ggtgatcgac gacaattcca cggacgatac gctggaaaag atggggcgtt
      661 acggagaaaa tgaccgggta cggtatatcc ggaatgagac aaacaaagga gcatccgcat
      721 ccagaaaccg cggtgtacag ctcgcaaagg gcgattacat tgcctttctg gacgcggatg
      781 attactggga tcccgaaaaa ctggaaaagc aggaaaaagc ccttgcaaag accggtgatg
      841 tgctctgctg tacgggtcgg gagctgattt acgatcagga accggaaaag cagaagatct
      901 tttctgtccc ggagcggatc acctacaaaa tgctgctgcg cggcaacgtg atcggctgct
      961 cgtcggtact ggccaaaaga agcgttctgc tggaattccc gatggaaaag gacgagtgcc
     1021 acgaggatta tctggactgg ctgcggatcc tgaaaaaata cgggacagcc tgcggtgtgg
     1081 atgagccttt gctgaaatac cggctgtcca gcacaggaaa atcggggaat aaactgcatt
     1141 ctgcaggaat gacttaccgt gtttaccgcg agctgggaat gggaacgttc cgctccctgt
     1201 actgttttgc gtgctatgcc gtaaacggtg tgcggaagta ttacggtaag ggcaaaaaga
     1261 gaggaagaga ttgatgaact ataaaaacag ggaacagtac aaacatctga tgcgtgtcct
     1321 ggcggtcgcg gcgattgtgc ttctggaatg cgctgttttt tctgtgttct gggacagata
     1381 ctatgcgagc cacctgtggc tggagccgtt ctgggataag ggaaatcaga ttatcgtcgt
     1441 gatctacggt atttttcttc tggttttcat gtacatttac ggcggcatga agatcggcta
     1501 catgaaggga agcagcatca tctattccca gatgatgacg gcattctgtg ccaatgcgct
     1561 gatgtatctg cagattatcc tgctgtggcg ccacctgccg tacattctgc cgatggtcgg
     1621 catgacactc gtggactttg cgctggcagg cattgtatcc tggctttttg agaagacgtt
     1681 tgcgcgtctc tttccgccaa gagaggtgct tctgatttac ggtgaatacc cgatggaaag
     1741 cctggagctg aagatgaacc agcggccgga caagtatatt gtagcacata aggtgagcgt
     1801 ggaagtggga gagaaagagc tctgccgcca gattgacgca tacggtcagg agggcgtgat
     1861 cctgggcgat cttcattcgg agctgcgcaa ccgtctgctg aaatattgct atgcgaaaga
     1921 aatccgtgtc tatctgacgc cgaagctgtc ggatattatg gtacggaaag cggaagcgct
     1981 ccatattttc gatacaccgc ttctgctcgc gcgaaacagt ggtctttcgt ttgatcagag
     2041 attctgcaag cgcctgctgg atctgctcgt atcgacgatt cttcttgtga tcacgtcgcc
     2101 gattatgctg atcgcagcgc tctgcatcaa gctgtatgac catggcccgg tgttctttaa
     2161 acagaaacgt ctcacgatcg gcggaaaaga attctatgtc tacaaattcc gaagcatgat
     2221 cgtggatgcc gaaaaagacg gcgtagcccg cctggcaacg aagaacgaca gccgcattac
     2281 gccggtcggg aaggtgatcc gcgcgacgag aattgatgag ctgccgcagc ttctgaacat
     2341 tttcaagggc gatatgagcg tcgtcggacc gcgcccggag cggccggaga ttgcaaagga
     2401 atatgaggaa tcgattccgg agtttgctta ccgtctgaaa gtaaaggcag gccttaccgg
     2461 ctacgcccag atttacggaa agtacaacac gaccgcatac gataa
//
LOCUS       DAOMUF010000248         2468 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_12139_length_2468_cov_2.790717, whole genome shotgun sequence.
ACCESSION   DAOMUF010000248 DAOMUF010000000
VERSION     DAOMUF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12139_length_2468_cov_2.790717"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PLN77_10120"
     CDS             complement(<1..173)
                     /locus_tag="PLN77_10120"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566946.1"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRM44660.1"
                     /translation="MNYKMILQYDGTRYNGWQKQGNTDNTIQGKLETLLSRIAGEPVE
                     IHGAGRTDAGVHAA"
     gene            complement(203..1642)
                     /locus_tag="PLN77_10125"
     CDS             complement(203..1642)
                     /locus_tag="PLN77_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRM44661.1"
                     /translation="MNIQEAKETIEKALRAYFARDEIGFPLVPLRQQRPILLIGPPGI
                     GKTAIMEQIAEECGVGLVAYTMTHHTRQSAMGLPEICTREIEGKTVHTTEYTMSEIIA
                     SIYDCMEQTGKKRGILFLDEINCVSETLAPVMLQLLQDKKFGNQHIPDDWLIVAAGNP
                     PEYNKSVREFDVATLDRVRKIEVLPELSVWLSYAEKNQIHSAVTTYLMAHSDHFYSVS
                     QEADEKRFVTARGWEDLSAVLKSYEILNFPVDEALIGQYLQEEKTAEEFSSYYRIYEK
                     YGQDYGVEEILTGAFSGKIMAEKQKMAANGSAEERSVLLSLFHAALQKEASGCQKQEH
                     TAKELSECFRQFTGLCRQKKDETYASWLQQKEQGFAVCKQQGLLKKGEEETNARVLKK
                     LKKLEETAKKCRKNDPDQMKELFETVCELEQEKAEKEVKDVKLQIRRAAEFLQNSFPG
                     GAEVVLFEANLARSPALRAFLIEKSMDAE"
     gene            complement(1730..>2468)
                     /locus_tag="PLN77_10130"
     CDS             complement(1730..>2468)
                     /locus_tag="PLN77_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HRM44662.1"
                     /translation="IESFQSVWKRASRKLAENYGTGGLRRGQNCGGKKETLSGIEKRA
                     YDYRRFLKRFTVTREEMELDLDSFDPVAYHYGLMYYGNFPFIEPPETREGNRLEELVI
                     AIDTSGSCKKEIVAKFLGETRSILEEKENFFSRWNVCLIQCDSFVQDVTMIHSPKEWE
                     AYRETITIKGRGGTDFRPVFQKVEELRKQHVFRNLKALIYFTDGDGIYPEQKPDYETA
                     FVFVRQTEKMNLVPKWAKKLLTEECTL"
BASE COUNT          501 a          671 c          552 g          744 t
ORIGIN      
        1 gccgcatgga cgccggcatc ggttctgccc gcgccgtgga tctccaccgg ctctcctgcg
       61 atcctggaca acagcgtttc gagcttgccc tgaatcgtgt tgtccgtatt tccctgcttc
      121 tgccagccgt tatagcgcgt gccgtcgtac tgcaggatca ttttataatt catctttttc
      181 tcctttccaa agtggccgct tatcattcgg catccatgct tttctcaatc aaaaaagccc
      241 gcagcgcagg acttcgcgcc agatttgctt caaacagcac cacttctgct ccgcccggaa
      301 agctgttctg taaaaattca gcggcacggc ggatctgcag cttcacatct tttacttcct
      361 tctccgcttt ttcctgttcc agttcacata ccgtttcaaa aagctctttc atctgatccg
      421 gatcgttttt gcgacatttt ttagcggtct cctcgagttt ttttaacttt ttcaagaccc
      481 gcgcattcgt ttcttcctcg ccctttttca gcagtccctg ctgtttgcac actgcaaatc
      541 cctgctcttt ctgttgtagc caggatgcgt atgtctcatc ttttttctgc ctgcaaagcc
      601 cggtaaactg gcgaaagcac tcggagagct cctttgccgt gtgttcctgc ttctggcagc
      661 cggatgcctc tttctgaagt gccgcatgaa acagagacag cagtacagat cgctcttctg
      721 cggaaccatt tgccgccatc ttctgttttt ctgccatgat ttttccggaa aatgcaccgg
      781 tcagaatctc ctctacaccg taatcctgtc catatttttc gtaaatcctg tagtacgagc
      841 tgaattcctc cgccgtcttt tcttcctgca aatattgtcc aatcagtgct tcgtcgaccg
      901 gaaaattcag gatttcataa cttttcagca ctgccgacaa atcctcccag ccgcgtgctg
      961 tcacaaaacg cttctcatcc gcttcctgcg aaacagaata aaaatggtca ctgtgtgcca
     1021 taaggtacgt tgtcacggcg ctgtggatct gatttttctc tgcgtaggag agccagacgg
     1081 aaagctcggg aagcacctcg atcttccgca cacggtcaag cgttgccaca tcaaattccc
     1141 gcacagattt attatattcc ggcggatttc cggcggccac gatcagccag tcatccggga
     1201 tgtgctgatt gccgaatttt ttgtcctgca aaagctgcag cattaccgga gcgagggtct
     1261 cggagacgca gttaatttca tcgagaaaga ggataccccg cttctttccg gtctgctcca
     1321 tgcagtcata gatcgaggcg atgatctcgc tcatcgtgta ttccgttgta tgtaccgttt
     1381 tcccttcgat ttcacgtgtg cagatctccg gaaggcccat ggcactctga cgcgtatggt
     1441 gtgtcatcgt gtaggcaaca agccccacac cgcactcttc tgcgatctgc tccataattg
     1501 ccgtttttcc gatgcccggc gggccgatca ggagaatggg acgctgctgc cggaggggaa
     1561 ccagcggaaa accaatttca tctcttgcaa agtatgcgcg cagggctttt tcaattgtct
     1621 cttttgcttc ttgtatgttc atatgttctc tcttatcttg tgcactcttt atgtactctc
     1681 gaaatctttc tgtgtttgat tttgcgagat gcttttttgt cagttgtgtt caaagagtac
     1741 attcttctgt cagcagtttt tttgcccatt ttgggacaag attcattttt tcggtctgtc
     1801 tgacaaagac aaatgccgtc tcgtaatctg gcttttgttc gggataaatt ccgtctccgt
     1861 cggtaaaata gatcaacgct tttaagtttc ggaatacatg ctgtttccgc aactcttcca
     1921 ctttctgaaa gacaggacgg aaatcggttc cgccccggcc cttgatggta attgtttcac
     1981 ggtaagcttc ccattctttc ggtgaatgga tcatcgtgac atcctgtacg aagctgtcgc
     2041 actggatcag gcagacgttc catcgggaaa agaagttttc tttttcttca agaatgctgc
     2101 gcgtctcacc gagaaatttt gctacgattt cttttttgca cgaaccggat gtgtcgatcg
     2161 cgataaccag ctcttcgaga cggtttccct ctcgcgtctc cggcggctcg atgaaaggga
     2221 aatttccgta atacataagc ccataatggt aagcgactgg atcgaagctg tcgagatcca
     2281 gctccatttc ctcgcgggta acggtaaagc gtttcaggaa gcggcggtaa tcgtacgctc
     2341 ttttttcgat cccggacagg gtttcttttt ttccaccgca gttctggcct cgccgcaggc
     2401 cgccggtgcc gtagttttcc gccaacttgc gggaagcacg tttccagact gattgaaaag
     2461 actcgata
//
LOCUS       DAOMUF010000249         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_12152_length_2466_cov_2.890502, whole genome shotgun sequence.
ACCESSION   DAOMUF010000249 DAOMUF010000000
VERSION     DAOMUF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12152_length_2466_cov_2.890502"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1374)
                     /gene="ilvA"
                     /locus_tag="PLN77_10135"
     CDS             complement(163..1374)
                     /gene="ilvA"
                     /locus_tag="PLN77_10135"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRM44663.1"
                     /translation="MLTLPKFEEASEIVKKVTQETKLVYSKYFSEQTGNKVYLKPENM
                     QLTGAYKLRGAYYKISTLSEEERAKGLITASAGNHAQGVAYAAKCYGAKAVIVMPTTT
                     PLIKVERTQSYGAEVVLYGDVYDEACAKAYELAAEHGYTFIHPFDDLTVATGQGTIAM
                     EVIQELPLVDYILVPIGGGGLATGVSTLAKLLKPNIKVIGVEPSGAACMKASFEKGEV
                     TTVSPVSTIADGTAVQTPGSKIFPYVRQNLDEIITVDDDELIVAFLDMVENHKMIVEN
                     SGLLTVAALKHLDVKGKKIVSILSGGNMDVITMSSVIQNGLIQRDRIFTVSVLLPDKP
                     GELVRVSQVIANENGNVIKLDHNQFFTTNRSAAVELRITMEAFGTDHKNRIVKALEEA
                     GYTPKIVRPNL"
     gene            complement(1411..2352)
                     /gene="ftsY"
                     /locus_tag="PLN77_10140"
     CDS             complement(1411..2352)
                     /gene="ftsY"
                     /locus_tag="PLN77_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRM44664.1"
                     /translation="MAEEKVGFFKRLVNGLTKTRDSIVAGIDSIFSGYSNIDDDFYEE
                     IEEILVMGDIGINTTTKIIDHLKERVAEEHITDPAECKQLLIESIKKQMDVGDTAYEF
                     ENRKSVVLVIGVNGVGKTTSVGKLAGLLKDQGKKVVLAAADTFRAAAGEQLTEWAHRA
                     GVDIIGGQNGADPASIVYDAVAAAKARNADVLICDTAGRLHNKKNLMEELHKIYRILE
                     KEYPEAYLETLVVLDGTTGQNALQQARQFAEVANVTGIILTKLDGTAKGGIAVAIQSE
                     LDIPVKYIGVGESIHDLQKFDADQFVNALFHTSEENA"
BASE COUNT          528 a          652 c          524 g          762 t
ORIGIN      
        1 aaagaatcag accagataaa atccagataa aaatttctgc tgctcttctt tttgtctttc
       61 taaactatct ttctttcaaa aaaaatcaga ggaaattctt tttcggatct cctctgattt
      121 ttatttttct ttttttcaga attttctctg caatttctgt ttttacaggt tcggtcttac
      181 aatcttcggt gtatagccag cctcttccag tgctttgacg atacggttct tgtgatcggt
      241 gccgaatgcc tccattgtga tgcggagctc tactgcagca cttctgttcg ttgtgaagaa
      301 ctggttgtgg tccagtttga tgacgtttcc gttctcgttt gcaatcacct gggaaacgcg
      361 taccagctcg cctggtttat ccggcagaag aacggataca gtaaagatac ggtctctctg
      421 gatcagtccg ttctggatca cggaagacat ggtgatgacg tccatatttc cgccgcttaa
      481 gatcgaaaca atctttttgc ccttgacatc cagatgcttt aatgcagcca cagtcagaag
      541 accggagttc tcaacgatca tcttgtggtt ttctaccata tcaaggaatg ctacgatcag
      601 ctcatcatca tccacggtaa tgatctcgtc caggttctgt cttacatacg ggaaaatctt
      661 ggatcccggt gtctgtactg cagtaccgtc tgcaattgtg ctgacaggag atacggtcgt
      721 tacctcgcct ttttcaaaag aagccttcat acaggctgct ccggacggct caacaccgat
      781 gactttgatg tttggtttca gcagttttgc cagtgtggaa acaccggtag ccagtccgcc
      841 tcctccgatc ggaacaagga tataatctac gagcggaagc tcctgaatca cttccatagc
      901 gatggttccc tggccggttg caacggtcag atcatcaaac gggtgaataa aggtatagcc
      961 atgctctgct gcaagctcgt atgcttttgc acatgcctca tcgtacacat ctccgtacag
     1021 aacaacctca gcgccgtagc tctgggtacg ctctactttg atgagcggtg tggtcgtcgg
     1081 catcacaatg acagcctttg caccgtagca ttttgccgca tacgcaacgc cctgtgcatg
     1141 gtttcctgca gatgcggtga tcagtccttt tgcgcgctcc tcttcactta aggtgctgat
     1201 tttgtaatag gcaccgcgca gcttataggc gccggtcagc tgcatatttt ccggttttaa
     1261 gtagacttta tttcccgtct gctcgctgaa atatttgctg taaaccagtt tggtctcctg
     1321 tgttactttt tttacaattt cagacgcttc ctcaaatttc gggagtgtca gcatttcttc
     1381 tgccatagtc ttttctcctt gttgtctttt ttaagcattc tcttcggatg tatggaacag
     1441 tgcatttaca aactggtctg catcaaattt ctgtagatcg tggatgctct cgcccacgcc
     1501 gatgtatttg accggaatat caagctcgga ctggatggca acggcgatac cgccctttgc
     1561 agttccgtcc agcttcgtca gaatgatacc ggtgacattt gccacttctg caaactgtct
     1621 tgcctgctgc agcgcattct ggcccgtggt accatccagc accaccagcg tttccaggta
     1681 tgcctctggg tactcttttt ccagaatccg gtaaatcttg tggagctctt ccatcaggtt
     1741 cttcttgttg tgaagtcttc ctgccgtatc acagatcagc acatccgcat ttctggcttt
     1801 tgccgctgcg actgcatcgt acacgatgga cgccggatct gctccgttct gtccgccgat
     1861 gatatccacg ccggcgcggt gcgcccactc cgtgagctgc tcgcctgccg ctgcacggaa
     1921 cgtatctgct gccgcaagaa caaccttttt gccctgatcc ttcagaaggc cggccagctt
     1981 tccgacggag gtcgtttttc cgactccatt gacaccgatc accagcacta ccgatttccg
     2041 gttttcaaat tcataggctg tatcgcctac atccatctgt tttttgatgc tctcgatcag
     2101 aagctgtttg cactctgccg gatcggtaat gtgctcttct gccacccgct cttttaagtg
     2161 gtcaataatt ttggtcgttg tattgatgcc gatatctccc atgacgagaa tctcctcgat
     2221 ctcctcataa aaatcgtcat caatattgga atagccgctg aaaatagaat caattccagc
     2281 tacaatgctg tctcgggtct tcgtcagacc gttgacaaga cgtttgaaaa agcctacttt
     2341 ctcctccgcc ataccgttcg cctcccttta tcttttctat cttacttctt tcgtattgta
     2401 tcttattcgt ttctatttgt ccagttctcc ctcgatcaaa tcaacagaaa caagggtaga
     2461 aacacc
//
LOCUS       DAOMUF010000250         2402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_12571_length_2402_cov_3.746059, whole genome shotgun sequence.
ACCESSION   DAOMUF010000250 DAOMUF010000000
VERSION     DAOMUF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12571_length_2402_cov_3.746059"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="PLN77_10145"
     CDS             complement(<1..1651)
                     /locus_tag="PLN77_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRM44665.1"
                     /translation="MEKNRIRPIPTGKSMRMSYQRQKEVLEMPNLIEVQKDSYDWFLR
                     SGLKEVFDDISPISDYGGRLSLEFVDFTLCEDDVKYSIEECKQRDATYAAPLKVKVRL
                     YNKEKDEITEHEIFMGDLPLMTATGTFVINGAERVIVSQLVRSPGIYYGIAHDKLGKR
                     LFSCTVIPNRGAWLEYETDSNDVFYVRVDRTRKVPITVLIRALGVSSNAEIVELFGEE
                     PKILASFTKDTSTNYQEGLLELYKKIRPGEPLAVENAESLIMSMFFDPRRYDLAKVGR
                     YKFNKKLALRSRIRNQILAEDVVDPSTGEILAEKGTTVTLELADKIQNAAVPYVWIQT
                     EERNVKVLSNLMVDLKAWVDVDEEELGINEKVYYPVLEKILEENTDIEEIKQAIKRDI
                     HDLIPKHITKEDIFASINYNMHLEYGAGNADDIDHLGNRRIRAVGELLQNQYRIGLSR
                     MERVVRERMTTQDIDGISPQSLINIKPVTAAIKEFFGSSQLSQFMDQNNPLGELTHKR
                     RLSALGPGGLSRDRAGFEVRDVHYSHYGRMCPIETPEGPNIG"
     gene            complement(2002..>2402)
                     /locus_tag="PLN77_10150"
     CDS             complement(2002..>2402)
                     /locus_tag="PLN77_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HRM44666.1"
                     /translation="LAKLFAKGGGKAYLYNFALEFPFQHGKPAWHCSDIPYFFGNADL
                     VEICGIPDVSDKLESEIFGALLAFAKNGTPDHAGLPHWPEVEAEDADTMVFDRKTEVK
                     HNYDDKVFAEINKVLKPWSFMDMMADNIQH"
BASE COUNT          537 a          567 c          549 g          749 t
ORIGIN      
        1 gaccgatgtt cggtccctca ggagtctcga tcgggcacat tcttccgtaa tgggagtagt
       61 gaacgtctcg aacctcgaat ccggcacggt cacgggacag accgccaggt ccaagggcgg
      121 aaagacgtct cttgtgcgtc agctctccaa gcgggttgtt ctgatccatg aactgggaca
      181 gctgggaaga tccgaagaac tctttgattg cagctgtaac cggtttgata ttgatcaggg
      241 actgcggaga aattccgtcg atgtcctgtg ttgtcatacg ctcacgtacc acacgctcca
      301 tacgggacag accgatgcgg tactggttct gaagcagctc acctacggca cggattctac
      361 ggttgcccag gtggtcgata tcatcggcat ttcctgcgcc gtattccaga tgcatgttgt
      421 agttgatgga agcgaaaata tcttcctttg tgatatgttt cggaatcagg tcgtggatgt
      481 cacgtttgat cgcctgtttg atctcttcga tatcggtatt ctcctccagg atcttttcca
      541 gaaccggata gtatactttc tcgttgatgc caagctcttc ctcgtcaaca tctacccatg
      601 ctttcagatc taccattaag ttggaaagaa cttttacgtt tctctcctcg gtctggatcc
      661 atacatacgg tactgctgcg ttctgaatct tgtctgcgag ttccagagta acggtggtgc
      721 ctttttctgc aaggatttca ccggtggacg gatctacaac atcctcagcg aggatctggt
      781 tgcggatacg gctgcgcagc gcaagtttct tgttgaattt ataacggcct acctttgcca
      841 ggtcatagcg acgcgggtca aagaacatgc tcatgatgag gctctctgca ttttctactg
      901 cgagaggctc gcccggacga attttcttgt acagctcgag aagaccttcc tgatagttgg
      961 tggaagtatc tttggtgaag cttgcaagaa tcttcggctc ttcaccgaac agctctacga
     1021 tctctgcatt ggagcttact ccaagtgcac ggatgaggac tgtgatcgga acttttctgg
     1081 tacggtctac gcggacatag aagacgtcgt tggaatcggt ctcgtactcc agccatgctc
     1141 cacggttcgg gattacggta caggagaaca gtctctttcc gagcttatca tgtgcgatgc
     1201 cgtaataaat accaggggaa cgtaccagct ggctgacgat tactcgctct gcaccattga
     1261 taacgaatgt tcctgttgct gtcatcaacg gcagatcacc catgaaaatt tcgtgctctg
     1321 tgatctcgtc tttttcctta ttatataatc ttacttttac ctttaacggt gccgcataag
     1381 tagcgtcacg ctgtttacac tcttcgattg agtattttac gtcatcttca cacagtgtaa
     1441 agtctacgaa ttcaaggctc agccgtccgc cgtagtcgga gattggtgaa atgtcatcaa
     1501 agacttcctt gagtccgctt ctcaggaacc agtcatagga atccttctgg acttcaatca
     1561 agttgggcat ttccaatact tctttttgtc tctggtaact cattcgcata ctttttccgg
     1621 taggtatcgg acgtattctg tttttctcca ttgacgttgt caccccttgt gttataatat
     1681 atttattacc gctcatgcct gaacaaaagg ttttaggcat atcttgccat aatagtgcat
     1741 tttctagatt atcatgtttt ttcaagtgtg tcaacaaaac agattgtatt tttttcagta
     1801 caatccgttt tttactggtt tttcttccgt gaccctgtta gaataatact ttagatgtga
     1861 ttgaaagcac cgaattttca gaaaaaaccg cctgtttgat ttttttattt ttcaaatcaa
     1921 acaggcggtt tttttattct aattttttaa tatttcagag ctccatcccg atttcgctta
     1981 tgccgcgtcg aatcgttctt tttaatgctg aatattgtca gccatcatgt ccataaagct
     2041 ccatggtttc agaactttgt tgatctctgc aaacacttta tcatcgtagt tgtgttttac
     2101 ttccgttttg cggtcaaaca ccatggtatc cgcatcctct gcttctactt ccggccagtg
     2161 cgggagtccg gcatgatccg gggtgccgtt ctttgcgaag gcaagaagtg ctccgaagat
     2221 ttcgctttcc agtttatcgc tgacatccgg gatgccacaa atttctacga gatctgcgtt
     2281 tccgaagaaa tatgggatat cggagcagtg ccatgccggc ttgccgtgct ggaacgggaa
     2341 ttccagagcg aagttgtaga gatatgcttt gccgccgcct tttgcgaaca gtttggcaag
     2401 tt
//
LOCUS       DAOMUF010000251         2393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_12631_length_2393_cov_2.750214, whole genome shotgun sequence.
ACCESSION   DAOMUF010000251 DAOMUF010000000
VERSION     DAOMUF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12631_length_2393_cov_2.750214"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLN77_10155"
     CDS             <1..883
                     /locus_tag="PLN77_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRM44667.1"
                     /translation="IKDDKGQVAGAVLQNMETGEYQVAKAKTVIIATGGAGRLHYNNF
                     PTSNHYGATADGLILGYRAGAPLLYQDTIQYHPTGAAYPAQIFGALVTEKVRSLGAML
                     VNVDGEAFMHPLETRDVSAASIIRECSDDRNEGVETPQGKAVWLDTPMIELIHGEGTI
                     EKRIPAMFRMFMQYGIDMRKEPILVYPTLHYQNGGLEINGEGFTNTVSNLLVAGEAVG
                     GIHGRNRLMGNSLLDVIVFGRDAGKAAAAKAKDVTLGKMNLDHVEKYAETLKEAGIDT
                     GMVSPQLLPDYAGKRHL"
     gene            1020..1862
                     /locus_tag="PLN77_10160"
     CDS             1020..1862
                     /locus_tag="PLN77_10160"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRM44668.1"
                     /translation="MREIQASTITDVVEKLCIEANYHLPEDVKCAITGCRACEDGEIA
                     KGVLDKIVENYEIADAQNVPICQDTGMACVFLEIGQDVHIAGGDLAEAVNEGVRRGYD
                     KGYLRKSVVKDPVRRGNTGDNTPAMLYTEIVPGEQIKVTVGPKGFGSENMSMIRMFKP
                     SAGLQGIKDFILEVVETAGPNPCPPMVVGVGIGGTFDKCALLAKKALMRPIDSENPDP
                     FYADLEKEMLEKVNKLGIGPQGFGGKTTAIGLNIETMPTHIAGMPCAVNINCHVTRHK
                     TEVI"
     gene            1865..>2393
                     /locus_tag="PLN77_10165"
     CDS             1865..>2393
                     /locus_tag="PLN77_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HRM44669.1"
                     /translation="MEKHIQLPLTEELAKTLHAGDTVYLTGTIYTSRDAGHKRMCEAL
                     ARGEKIPFDPTDATIYYVGPTPAKPGQVIGSAGPTTSGRMDAYAPTMMSVGARGMIGK
                     GARLPEVVEAMKKYHGVYFGAIGGAGALLAKCIKKAELIAYEDLGAEALRRLEVEDMP
                     LFVIIDSDGNNLYEMG"
BASE COUNT          695 a          566 c          644 g          488 t
ORIGIN      
        1 gatcaaagac gataagggac aggttgcagg tgctgttctc cagaacatgg agactggcga
       61 gtaccaggta gcaaaggcaa aaaccgttat catcgcaaca ggtggtgcgg gacgtcttca
      121 ctacaacaac ttcccgacat ccaaccatta cggtgcaacc gctgacggtc tgatccttgg
      181 ataccgtgca ggcgctccgc ttctgtatca ggataccatt cagtaccatc cgaccggagc
      241 tgcttacccg gctcagatct tcggtgctct ggtaaccgag aaagtacgtt ctctgggcgc
      301 aatgctcgta aacgtagacg gcgaagcttt catgcatcca ctggaaaccc gtgacgtatc
      361 cgcagcaagc attattcgtg aatgctccga cgaccgcaac gagggcgttg agactccaca
      421 gggcaaggct gtatggctgg atactccaat gatcgagctg atccacggag aaggaaccat
      481 cgagaagaga atcccggcta tgttccgtat gttcatgcag tatggtatcg atatgagaaa
      541 agagccgatc ctggtttacc cgacgctgca ttatcagaac ggtggactgg aaatcaacgg
      601 cgaaggcttt accaacaccg taagcaacct gctcgtagca ggtgaggcag ttggaggaat
      661 ccacggaaga aaccgtctga tgggtaactc tctgctggac gttatcgtat ttggccgtga
      721 tgcaggaaaa gctgcagcag ccaaagcaaa agacgtaaca cttggaaaga tgaatctgga
      781 tcacgttgag aaatacgctg agaccttaaa agaggcaggt attgataccg gtatggtatc
      841 tccgcagctt ttgccggatt atgcaggtaa aagacacctg taaaatgcag taaccgcgaa
      901 aaagcgtgtc ggaagggggc ggcgtttaaa agccgcttcc gggacacgct ttccgtgctg
      961 aaatccaaaa ggtttttcgg tacgataaat catagaagta aagaaaggtg gagaacccca
     1021 tgcgtgaaat tcaggctagt accattacgg atgtggtaga gaaactttgt atcgaagcaa
     1081 actaccatct tccggaagac gtaaaatgtg ccatcacagg ctgccgtgcc tgcgaggatg
     1141 gcgagattgc aaaaggtgtt ctggacaaaa tcgtagaaaa ttatgagatc gcagacgcac
     1201 agaacgtgcc gatctgccag gataccggaa tggcatgtgt tttcctggaa atcggacagg
     1261 atgtgcacat cgcaggagga gatcttgcag aggcagtcaa cgaaggtgtc agaagaggat
     1321 atgacaaagg atacttaaga aaatccgttg tcaaagatcc ggtcagaaga ggaaacaccg
     1381 gcgacaatac accggctatg ctctacaccg agatcgttcc tggcgagcag atcaaagtta
     1441 ccgtaggccc gaaaggcttc ggaagcgaaa atatgagtat gatccgtatg ttcaaaccgt
     1501 ccgcaggtct tcagggaatc aaggatttca tcctggaagt tgttgagaca gcaggcccga
     1561 acccatgccc gcctatggta gtcggcgttg gtatcggcgg aaccttcgat aaatgtgcac
     1621 tgcttgcgaa aaaagcactg atgcgcccga tcgattccga gaacccggat ccgttctacg
     1681 cagatctgga aaaagaaatg ctggagaaag tcaacaaact cggcatcgga ccgcagggct
     1741 ttggcggaaa gaccacagcc atcggcctca acattgagac aatgccgact catatcgcgg
     1801 gaatgccgtg tgcagtcaac atcaactgcc atgttacccg tcataaaacg gaggtgatct
     1861 gatcatggaa aaacatattc agctgccttt aacagaagaa ctggcaaaaa cactgcatgc
     1921 cggagatacc gtatatctga ccggaactat ctatacttcc cgtgacgccg gacataagag
     1981 aatgtgtgaa gcgctcgcaa gaggagaaaa aatcccgttt gatccgacag acgctacgat
     2041 ttactacgta ggaccgaccc ctgcaaaacc aggccaggtc atcggatctg caggtcctac
     2101 caccagcggc cgtatggatg cgtatgctcc gaccatgatg tccgtaggcg caagaggaat
     2161 gatcggtaaa ggcgcacgtc ttccggaagt tgtagaagcc atgaagaaat atcatggcgt
     2221 atacttcgga gccatcggcg gagccggcgc actgcttgca aaatgcatca aaaaagcaga
     2281 gctgatcgca tacgaagatc ttggcgcaga agcacttcgc cgtctggaag tagaagacat
     2341 gccgcttttt gttatcatcg acagcgacgg aaacaatctg tatgagatgg gaa
//
LOCUS       DAOMUF010000252         2388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_12661_length_2388_cov_3.045864, whole genome shotgun sequence.
ACCESSION   DAOMUF010000252 DAOMUF010000000
VERSION     DAOMUF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12661_length_2388_cov_3.045864"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLN77_10170"
     CDS             complement(<1..1183)
                     /locus_tag="PLN77_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM44670.1"
                     /translation="MNDKIAIYLRLSLADGDLKKGSKDESNSIENQRMLLHDYIGKQE
                     DLFGEIVEYVDDGYTGTNFNRPAFQKMIVDLKQGDIKVIMVKDLSRLGRDYIGVGDYI
                     EQIFPLMGVRFIAVNNSFDSMKLNNGTPGIEVAVSNLVNNMYSRDIAKKIRAALETNW
                     KNGKATCTNVPFGYVWNKKGGQRWEIDPEAAPCVKKVFELALSGRNTTQIAYGMNELN
                     LPTPGLYAKRKNLLMGSNPIIAPDSEMLWNAAIVWRILRRYEYTGALVMGRRKKIDVN
                     TTSVRTLPEDKWIIAENAHAAIVTKDEYYQAQKAIRNVTPIQYKVGDDFALKGKICCG
                     NCNRQLRHERQYGEMVFYCGYKRSAGKFSKCYGGYYREYSVNAKVARAIKTVFYALDV
                     VN"
     gene            complement(1176..>2388)
                     /locus_tag="PLN77_10175"
     CDS             complement(1176..>2388)
                     /locus_tag="PLN77_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM44671.1"
                     /translation="YAKDISKKISLSYQMRREKGIPTSWCTPYGYQLNQQGNKFEATE
                     DAKWVKLIYQWYLAGVSTNEIARRLEFLEVARPNERLNRKLHEGDDPTYNKWHPSTVL
                     RILNSSAYIGELVSGKTQTASYKGIGLHPVEKKEWHIVENAHEAIILKSDFEKVQARR
                     EQNKEKRERAMARSKATREKCYNHLSGMVYCGCCRRNMTFERRVHGTVKETHYGVFIC
                     KRKKNTTPCAYHAVPEKMLMMVAMDQIHHLVSTMCEEEKLVKDMMRSSNLDSARSIKM
                     KENSILFRIQEAEERRLRLYEDYKAEILDEDEYSQLKEHYIAEKQRLEHELQKQRQRA
                     LELEKRIKICDAQMERMRGILNQNEFDEELVHELIKRIYVGMDNSVEVEFKCSDPYQE
                     VLAIMSEVQNE"
BASE COUNT          553 a          646 c          433 g          756 t
ORIGIN      
        1 gatttaccac atccagcgca tagaatactg tttttatcgc acgagccact tttgcattca
       61 ccgaatattc tctgtaatag ccgccgtagc atttagagaa ctttccggct gaccgtttat
      121 agccgcaata gaaaaccatt tccccatatt gcctttcgtg gcgaagctgc cgattacagt
      181 ttccgcagca gatttttcct tttaacgcaa aatcatcacc caccttatat tgaattggag
      241 ttacgttacg gattgctttc tgtgcctggt agtattcatc ttttgtcacg atggccgcat
      301 gagcgttttc tgcgatgatc cacttatcct ccgggagcgt ccgaacagag gtagtattca
      361 catcaatttt ctttcttctc cccataacca acgcaccagt gtattcatac cgtctaagga
      421 ttctccacac gattgccgcg ttccataaca tttcactgtc cggggcaata ataggattgc
      481 tgcccatcag cagattcttt cgtttcgcat acaatcccgg cgtaggaaga tttaattcgt
      541 tcatgccgta ggcgatctgc gttgtattgc ggccggacaa tgctaattca aatacttttt
      601 tcacgcaggg tgctgcctcc gggtcaatct cccaccgctg cccacctttc ttgttccaca
      661 catatccaaa agggacattc gtgcaggtgg ctttcccgtt cttccagttc gtttccagag
      721 cagcccgaat ctttttcgca atatcccggc tatacatatt attcaccaga ttgctgaccg
      781 ccacttcgat tcccggtgtt ccgttgttca gtttcatgct gtcaaaggaa ttattcacag
      841 caataaaccg aactcccatc aacggaaaaa tctgttcaat gtaatcgcca acaccgatat
      901 agtcacgacc aaggcgggat aaatccttta ccatgatgac ttttatatcg ccctgcttca
      961 aatccacgat cattttctga aaagccggac gattaaaatt tgttccagta tagccatcat
     1021 ctacatattc tacaatctcg ccaaacaaat cttcctgctt cccaatgtaa tcgtggagca
     1081 gcatccgttg gttctcaatg ctgttacttt catccttgct gccctttttc aaatctccgt
     1141 ctgccaatga taagcggaga tagattgcaa ttttatcatt cattctgaac ctccgacatg
     1201 attgcaagca cttcctgata ggggtcgcta cacttaaatt cgacctccac agaattgtcc
     1261 attcctacat agattctctt gataagttca tgcaccagtt cttcatcaaa ttcattttga
     1321 ttcagaatcc cgcgcatccg ctccatctgc gcatcgcaga ttttgattct cttttccagc
     1381 tccaatgcgc gctgacgctg cttttgcagt tcatgttcca accgctgctt ttctgctatg
     1441 tagtgttcct ttagctgact atattcatct tcgtccagaa tttcagcttt atagtcctca
     1501 tataaacgca accgccgttc ttcggcttcc tgaatccgaa acaggataga gttttctttc
     1561 attttgatgg aacgagccga atccagattg ctgctgcgca tcatgtcttt taccagcttt
     1621 tcttcttcac acatggtaga caccagatga tgaatctgat ccatcgccac catcatcagc
     1681 attttctccg gcaccgcatg ataggcacag ggagtcgtgt tcttctttct tttgcaaatg
     1741 aacactccat agtgcgtttc tttcaccgta ccatgcacac gccgttcaaa cgtcatgttg
     1801 cgtctgcagc agccacagta aaccattccc gatagatggt tataacactt ctctctggtt
     1861 gctttggaac gagccatcgc cctttcccgt ttttccttat tctgctcccg ccgtgcctgc
     1921 actttttcaa aatccgattt cagaatgatt gcttcatgcg cgttttcaac aatatgccat
     1981 tccttctttt ccactggatg gaggccaatc cctttgtatg atgcagtttg cgttttccca
     2041 gataccagtt ctccaatata ggcactgctg tttaaaatac gaagaaccgt actggggtgc
     2101 catttattat aggtagggtc atctccctca tgtaatttgc gattcagccg ttcgttcggc
     2161 cttgctactt ctaaaaattc caacctgcgg gcaatttcgt ttgtactcac tcctgcaaga
     2221 taccattgat agatcagttt gacccacttc gcatcctcag tcgcttcaaa cttatttccc
     2281 tgttggttca actgatagcc atacggtgta caccacgatg tagggattcc cttttcgcgg
     2341 cgcatctggt aactaaggct gattttcttt gaaatgtcct tcgcgtac
//
LOCUS       DAOMUF010000253         2371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_12795_length_2371_cov_2.641623, whole genome shotgun sequence.
ACCESSION   DAOMUF010000253 DAOMUF010000000
VERSION     DAOMUF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12795_length_2371_cov_2.641623"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLN77_10180"
     CDS             complement(<1..966)
                     /locus_tag="PLN77_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44672.1"
                     /translation="MKDESFRPNRMLSYFQEEWKILFFVTVSGLIYNLGLLAGPWFEG
                     KMTGKLVDLLGGTGTFHQMLMLVTAYVVSIGIVQVSRYFKRFYVRRFANNVNRHMKQI
                     LYGTLVRKSRLELEAEGTGNVLTKAILDVDDCVEGMRKFTTEIFDTGVALLAYACMLL
                     WYDWRLALLCMIFPPISYVIAEKMKVMVQKTGAAYKVQSGALSAATLDRATNAITYRV
                     FGCEEDRKAAYEENLSAYESAAVRANIWNTAMPPIYRMISMTSILFILYFGGRNVLHE
                     GWTPWTIAVFTTFLSCYTKLATKSSSAAKLFNAVHKAQVSWKRIKP"
     gene            complement(956..1468)
                     /locus_tag="PLN77_10185"
     CDS             complement(956..1468)
                     /locus_tag="PLN77_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM44673.1"
                     /translation="MDPTERKITKIAREAGKFTVQAMKEEGIGTAEFDFIHLVRHHPG
                     ITQTEVREQLKIDKGAAARRAASLEAKGYLIRKPNPWDGRSQLLYATEKAESLKNSKA
                     EIETFFYEWLLAELPEKEAESFCETLDKLYWKSKRERQNHFAELSKILREKADKTIKA
                     TAEGGSGDEG"
     gene            1659..>2371
                     /locus_tag="PLN77_10190"
     CDS             1659..>2371
                     /locus_tag="PLN77_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRM44674.1"
                     /translation="MEKKHTRGSFTGSIGFVLAAAGSAVGLGNIWRFPYLAAKDGGGT
                     FILVYIVLALTFGFTLLTTDIAIGRKTGQSPLVAYGKIHPGWNGLGLLASIVPVVILP
                     YYCSIGGWVLKYLSVFLTGHGTAAAEDGYFTGYITGTWQPIVWLGIYLFLTAFVVYRG
                     VNKGIENYSKILMPILLILIIGISVFSLTLTHTDADGVVRTGLQGMKIYLVPNFKGMT
                     PQKFFTVFVDALGQLFFSI"
BASE COUNT          562 a          585 c          529 g          695 t
ORIGIN      
        1 aggtttgatt cgtttccagg aaacctgcgc tttatgtacc gcattaaaaa gctttgctgc
       61 gctcgatgat ttggtggcaa gcttcgtgta gcaggacaaa aatgtggtaa atacagcaat
      121 ggtccacggt gtccaaccct catgcaggac gttcctgcct ccaaaataca ggataaacag
      181 aatgctcgtc atcgagatca tacggtaaat cggcggcata gctgtgttcc agatattggc
      241 gcgcacagcg gcagattcgt aagccgaaag attctcctca taagcggcct ttctgtcctc
      301 ctcacagcca aagacacgat acgtgatggc atttgtcgca cggtccaggg ttgcagcact
      361 taatgcaccc gactgtacct tataggcagc gcccgttttc tgtaccatga ccttcatctt
      421 ttcggcaatc acgtaggaaa tgggtgggaa aatcatgcag agaagagcca ggcgccagtc
      481 gtaccagagc agcatacacg cataggcgag aagagcaaca ccggtatcaa agatttccgt
      541 tgtaaatttc cgcattcctt ctacgcaatc gtctacgtcg agaatggcct tcgtcagtac
      601 atttccggta ccttccgctt ccagctccaa acggcttttg cgaaccagcg ttccgtaaag
      661 aatctgcttc atatgccggt tgacgttgtt ggcaaatctt ctgacataga accgtttgaa
      721 atagcgggaa acctgcacaa tgccaatact cacgacatag gccgtgacca gcatcagcat
      781 ctggtggaag gttcctgtgc cgccgaggag atcaaccagc tttcctgtca tttttccctc
      841 aaaccagggg cctgcaagaa gtcccagatt atagatgaga ccggaaaccg taacaaaaaa
      901 gagaattttc cattcttcct gaaagtaaga gagcatccgg ttcgggcgaa aggattcatc
      961 cttcatcgcc gctgcctcct tctgctgttg cttttattgt tttatctgct ttttccctta
     1021 aaatttttga aagttccgca aaatgattct gccgttctct cttcgatttc cagtacagct
     1081 tgtccagtgt ttcgcagaag ctctctgcct ctttttccgg aagctctgca agcagccatt
     1141 cgtaaaagaa tgtttcaatt tccgctttgg aatttttcaa ggattctgct ttttccgtag
     1201 catacagaag ctggcttctg ccgtcccacg gattcggttt ccggatcaga tatccctttg
     1261 cctcaaggct tgcagcgcgt cttgcagcgg ctcctttatc aatctttaac tgttcgcgca
     1321 cttccgtctg cgtgatgccc ggatgatggc gcaccagatg gataaagtca aattctgctg
     1381 tgccgatgcc ctcttctttc attgcctgaa ccgtaaattt cccggcttcc cgcgctattt
     1441 ttgttatttt ccgttctgtt ggatccattt ttctgttcct cctgtccatt ctcgatctgt
     1501 ttcaaataga tgcatataca actaattaga tgatagtaca actaattaaa attgtcaatc
     1561 gctagatctt ctggttgacg aagtatcgtc cggactttat aatagaagta ttcggctgtt
     1621 aaaaacagtc agtctatcaa taaggagatg tatcttatat ggaaaagaaa catacgcgcg
     1681 gcagctttac cggatcgatc ggcttcgtgc ttgctgctgc aggaagcgcg gttggactgg
     1741 gaaatatctg gcgtttccct tatcttgccg caaaagacgg aggcggaacc tttattttag
     1801 tttatatcgt actggctctg acctttggat ttaccctgct gacaactgat attgccatcg
     1861 gaagaaaaac cggacaaagt ccgcttgtcg cttacggtaa aattcacccg ggctggaacg
     1921 gtcttggcct tctcgccagt atcgtgccgg ttgtcatcct gccttactac tgctccattg
     1981 gcggctgggt attaaaatac ctgtctgtgt ttctcaccgg acatggcact gctgctgccg
     2041 aggatggtta ctttaccggc tatattaccg gtacatggca gccgattgtg tggttgggaa
     2101 tctatctttt tcttaccgca ttcgttgtat atcgtggtgt caataaggga attgaaaact
     2161 acagcaaaat tctgatgccg atccttctga ttttaatcat cggaatttct gttttttctc
     2221 tgaccctgac ccataccgac gcagacggtg tcgtccgtac gggactgcag ggtatgaaaa
     2281 tttatcttgt tccaaatttt aaaggaatga cgccgcagaa atttttcacc gtctttgtcg
     2341 atgcactcgg acagcttttc ttttcgatca g
//
LOCUS       DAOMUF010000254         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_12857_length_2362_cov_4.430863, whole genome shotgun sequence.
ACCESSION   DAOMUF010000254 DAOMUF010000000
VERSION     DAOMUF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12857_length_2362_cov_4.430863"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLN77_10195"
     CDS             <1..587
                     /locus_tag="PLN77_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM44675.1"
                     /translation="HCEYLINSLIGFFIGPAAAFDSYHMQDENGLWSGYGYEMMQGIA
                     KYMQCTFSYVGYDKTAKECEEMLQNGEADIYTAAKKTPEREGEFAFSRHPAITAFTSM
                     NVETPNWRSDLYNKYYGSVEVNTDYTAEEKALLSQLKESGAVIRGVMNPDGNPYSWYE
                     NGEAKGIAAARCVGKTAENSGNRRWCDGQRNPEQ"
     gene            559..981
                     /locus_tag="PLN77_10200"
     CDS             559..981
                     /locus_tag="PLN77_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44676.1"
                     /translation="MTVKEILNNEWPNAEIVSVKDTDECVQRLVNENVDGALLMTYTA
                     QKLARDDTQNRLRVEAVPGALMSLRMGVLSEVDRSFYGLWEKTLYNVSRKSRAEIVQS
                     YVEDVETPTIMAYLFDHPLYLVALIAGVLLFCLRRIMH"
     gene            1002..1418
                     /locus_tag="PLN77_10205"
     CDS             1002..1418
                     /locus_tag="PLN77_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM44677.1"
                     /translation="MLGSLGLVVDWAENGSEAVDQFEASDPGSYFAIFMDMQMPVMEG
                     IEATKKIRNSVREDNDVPIFAMTANTFATDRKRCADAGMTRAYSETGEFKRDSDDAGR
                     ESADGMRRITGRINNRKIWRRGSTALDCSGAFLCVS"
     gene            1450..>2362
                     /locus_tag="PLN77_10210"
     CDS             1450..>2362
                     /locus_tag="PLN77_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3878 family protein"
                     /protein_id="HRM44678.1"
                     /translation="MEIAENITWTEELERLDVLLSQGQFELLLPGERYGGGEKEIWLV
                     YLMNDAAESFLVFHDAELTGTYQKEYEGEIDAALEKDGEQYVLIVRQGETVCTLFFSR
                     LSLEVHLFDYGKTGHFWVDGYEYLRQIEFRIAILRDKLEYLGEAFCTEEEMRLASLAN
                     FPPLNFCCYPAVPDQYLVPSCPWWEATEEAITEMKKLASEAGDKVLLRYLALYEKWQG
                     KLLAKQIAKLLHTSRHAKVVDLLEKKLAREAQNYPKRRFTGEEGTQIRKIQEQAMKRK
                     KILEAEGKRASLLREEPFFYARDSVEYK"
BASE COUNT          648 a          492 c          734 g          488 t
ORIGIN      
        1 tccattgtga gtacctgata aattctctta taggcttttt cataggtcca gcagcagcct
       61 tcgacagcta tcacatgcag gacgaaaacg gactgtggag cggatatgga tatgagatga
      121 tgcagggcat tgccaaatat atgcagtgta cgttttccta cgtcggatac gataagacgg
      181 cgaaggaatg tgaggagatg ctgcaaaatg gagaagcaga catttatacg gctgccaaaa
      241 agacaccgga gcgcgagggg gagtttgcat tttcccgcca tccggcgatc accgcattta
      301 cgagcatgaa cgtggagaca ccgaactggc ggtccgatct gtacaataaa tattacggtt
      361 ccgtggaagt gaataccgat tacacggcag aggaaaaagc gcttctttcc cagctgaaag
      421 agagcggcgc cgtgatccgc ggcgtgatga atccggacgg aaatccatac tcgtggtatg
      481 aaaacgggga ggcaaaagga attgcggcgg cgcggtgcgt cgggaaaact gcagagaata
      541 gcggtaatcg acgatggtgt gacggtcaaa gaaatcctga acaatgaatg gccgaatgca
      601 gaaattgtgt cggtgaaaga tacggacgaa tgtgtgcaga gactggtcaa tgagaatgtg
      661 gacggtgcac tgctgatgac ctacacagcg cagaagctgg caagagatga tacgcagaac
      721 cgcctccgcg tggaagctgt gccaggtgca ttgatgtccc tgcggatggg cgtactctca
      781 gaggttgacc ggagtttcta cggtctgtgg gaaaaaacgc tttacaacgt atcacgaaag
      841 agcagagcgg aaattgtgca gagctatgtg gaagatgtgg agacaccgac catcatggcg
      901 tatctgtttg accacccgct gtatctggtg gcactgattg caggtgtgct gctgttctgc
      961 ttgcggagga taatgcactg aatgcagagg ttgcgacgga gctgctcgga agcctcggcc
     1021 ttgtcgtgga ctgggcggag aatggttccg aggccgtgga tcagtttgaa gcgtcggatc
     1081 cgggaagcta ttttgcgatc ttcatggata tgcagatgcc ggtgatggag ggcatcgagg
     1141 cgacgaaaaa aattcgaaac agtgtgcgtg aggacaatga tgttccgatt tttgcgatga
     1201 cagcaaatac ctttgcaacg gaccgcaagc ggtgcgcgga tgcgggcatg acgcgggcat
     1261 attccgaaac cggtgaattt aaaagagatt cagacgacgc tggacgagaa agtgcggacg
     1321 gcatgcgccg gataacggga cggattaata acagaaaaat ttggagaagg ggctctactg
     1381 ctttggattg cagtggagcc tttttatgtg tatcatagga agtaaaagaa aacaggggag
     1441 aatcacggaa tggaaattgc agaaaatatc acatggaccg aggaactgga aaggctggat
     1501 gtgcttcttt cgcaggggca gttcgagctg ctgcttccgg gtgagcggta cgggggagga
     1561 gaaaaggaga tctggcttgt ctatctgatg aacgatgcgg cagagagctt tctcgttttt
     1621 catgacgcgg agctgacggg aacgtatcag aaggaatacg agggagaaat agatgccgca
     1681 ctggaaaaag acggggagca gtacgttctg atcgtgcggc agggagagac ggtgtgcacg
     1741 ctgtttttca gccgcctttc actggaagtg catctgtttg attacggaaa aacgggtcat
     1801 ttctgggtgg acggatacga gtatctgcgg cagattgagt tccgtattgc gatcctgcgg
     1861 gacaagctgg aatacctcgg cgaggcgttc tgcaccgagg aagaaatgcg tctggcatcg
     1921 ctggccaatt tcccgccgct caatttctgc tgctacccgg ccgtgccgga tcagtatctc
     1981 gtaccgtcat gcccgtggtg ggaagcgacg gaagaggcaa ttacggagat gaaaaaactg
     2041 gcatcggagg caggcgataa agtgctgctg cggtatcttg cactttatga aaaatggcag
     2101 ggaaaactcc tcgcaaaaca gatcgcaaaa ctgctgcata cgagccgcca cgcgaaagtc
     2161 gtggatttac tggaaaaaaa actggcccgc gaagcacaga attacccgaa acgacgcttc
     2221 accggcgaag aaggaaccca gatccgaaaa atccaggagc aggcaatgaa acggaaaaag
     2281 atactggaag ccgaaggaaa acgcgcatca ttgctgcgcg aggagccatt cttttatgca
     2341 cgggattcgg tggaatataa gg
//
LOCUS       DAOMUF010000255         2348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_12951_length_2348_cov_4.485826, whole genome shotgun sequence.
ACCESSION   DAOMUF010000255 DAOMUF010000000
VERSION     DAOMUF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12951_length_2348_cov_4.485826"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLN77_10215"
     CDS             <1..734
                     /locus_tag="PLN77_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM44679.1"
                     /translation="PEWAFADAVGLLSQDYHVIQIVYDGHGETGEDFISVETTVDEIT
                     DWLQAHGITRLNAAYGCSLGGACLTRFLALGKIPVERAVIDAGITPYRMPLILRRLAC
                     LRDDLGFRLIAKNRKILETVYPPERWTMPGRDPVKEYDVLAAYLKTYSKRTVRNIFWS
                     ANNYTLPTKPAEMGCQITYWYGEEEKRARHSNVSFIKQYFPWVELHEIPKMDHAELVM
                     MYPQEFYRRIMEFLTHTSAAQNERN"
     gene            748..1455
                     /locus_tag="PLN77_10220"
     CDS             748..1455
                     /locus_tag="PLN77_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HRM44680.1"
                     /translation="MSKKATEFHFDRSCHVLYSKPCKKEIQAKIALHYPEAEREAVWE
                     QVQRQYVDFLSDWRTDLGGKRNFHNGAGGTYDCIAIMSYYAACKAVTSFREIEEIEEN
                     LILPTFRKLKFVDCNKPFWKKLMYKSFVRAKAGCDKWHDYEMTVAPYEKNKPIYYEFT
                     ACPAAEFAIRHDLTEIMPALCNVDFAAMELLHARLVRTTTCANGCKCDYTICGDQDPY
                     LKEHPEYRDENGYRRNR"
     gene            1455..2228
                     /locus_tag="PLN77_10225"
     CDS             1455..2228
                     /locus_tag="PLN77_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44681.1"
                     /translation="MLFQTYGDRNHPAVLFFHAMGVTGESSEPVAKYLQDRYFCILPT
                     STVYCKGQKYVSKADEVRQVEAYLKSQGVEHLELVVASSIGADLAMAFLTSTELPIGH
                     VFFDGGQFAQIGKGTRRMMTPFLYLAIKSLYWSKGGTLKKILWCDDDSIKLYFIAASE
                     NLTYTNLRRQLSDSLEDKPFPSLPEELQKQIYFEFGSIEDHFKYRQAVMEAYPCGHYP
                     VFEGYNHMQYQIRDPRGFAEMLTSIAEHDGMPKLPFIRK"
     gene            2240..>2348
                     /locus_tag="PLN77_10230"
     CDS             2240..>2348
                     /locus_tag="PLN77_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM44682.1"
                     /translation="MKYKIEKNTVQETLILPLYSRKLCSELYPNLYRDET"
BASE COUNT          625 a          563 c          635 g          525 t
ORIGIN      
        1 agccggagtg ggcgttcgcc gacgcggttg gtctgctttc gcaggattac catgtgattc
       61 agatcgtcta tgacggccac ggcgaaacgg gagaagactt tatctccgtg gagacgacag
      121 tagacgagat aacagattgg ctgcaagcgc acggcattac ccgcttgaac gcggcatacg
      181 gctgttctct gggcggtgcg tgtctgacac gctttctcgc cttgggaaaa atcccggtcg
      241 agcgcgccgt cattgatgcg ggcattacgc cgtatcgaat gccgctgatt ttgcgccgtc
      301 tcgcctgcct gcgtgacgat cttggtttca ggctcatagc gaagaaccgc aagattcttg
      361 aaacagtcta tccgccggag cgctggacga tgccgggacg cgatccggtg aaggaatacg
      421 acgtgctggc ggcgtatctg aaaacctatt caaaacgcac cgtccgcaac atcttctggt
      481 cggcgaataa ctatactctg ccgacgaagc cggctgaaat gggctgccaa attacgtatt
      541 ggtacggcga agaggaaaaa cgggcgcggc acagcaatgt cagctttatt aagcaatatt
      601 ttccatgggt ggaactgcat gaaattccga agatggatca cgcggagctt gtgatgatgt
      661 atccgcagga attttaccgg agaatcatgg agttcctgac acacacatcg gcagctcaaa
      721 acgaaagaaa ctgaaggaga aacggaaatg tccaagaagg caaccgaatt tcatttcgac
      781 cgcagctgtc atgtgctgta ttccaagccc tgcaagaaag aaatacaggc aaaaatcgct
      841 ctgcactatc cagaggccga gcgagaggct gtttgggaac aggtgcagcg gcagtatgtg
      901 gattttcttt cagactggcg cacggatctg ggcggtaaac ggaactttca caacggcgca
      961 ggcggcacct atgactgcat cgccatcatg agctactatg cagcctgcaa agcggttaca
     1021 tccttccgcg aaatcgagga gatcgaggaa aatctgatcc tgccgacctt ccgcaagctg
     1081 aaattcgtgg actgcaacaa gccattctgg aagaaactga tgtacaagtc attcgtacgg
     1141 gcaaaagcgg gctgtgacaa atggcacgat tatgagatga cggtggctcc ttacgaaaag
     1201 aacaagccga tctattatga atttaccgcc tgtcctgcgg cagaatttgc catccggcac
     1261 gaccttacgg agatcatgcc cgccctgtgc aatgtggatt ttgcagcgat ggaactgctt
     1321 cacgcaaggc tggtgcggac aaccacctgt gcgaatggct gtaagtgtga ctacaccatc
     1381 tgcggcgatc aggacccata cttaaaggaa catcccgaat accgggacga aaacggatac
     1441 aggaggaaca ggtaatgctg tttcaaacat acggcgacag gaatcatcct gcggttttgt
     1501 tttttcatgc aatgggcgtg acgggggaga gcagcgaacc ggttgcgaaa tatttacaag
     1561 atcggtattt ctgcattctg cctacctcca ccgtgtactg caaagggcag aaatatgtca
     1621 gcaaggcgga cgaggttcgt caggtagaag catacctgaa atcgcaggga gtggaacacc
     1681 ttgaactggt tgtggcttct tcgatcggtg ccgatcttgc catggcgttt ttgacaagta
     1741 cagaattgcc cattggacac gttttcttcg atggcgggca gtttgcgcaa atcggaaaag
     1801 gtacgcgccg catgatgaca ccgtttttgt atctggccat caagagcctg tattggtcaa
     1861 agggcggcac actgaaaaag attttgtggt gtgacgatga ctccataaag ctgtatttta
     1921 tagccgcaag cgaaaatctg acctacacaa atttgcggcg gcagctttcg gacagcctgg
     1981 aggataaacc ctttccgtca cttcccgaag aactgcaaaa gcagatctat tttgagtttg
     2041 gcagcataga agatcatttc aaataccgtc aggcagtgat ggaagcctac ccctgcggcc
     2101 attatcccgt gtttgaggga tacaatcata tgcaatatca gatacgcgat ccgaggggct
     2161 ttgctgaaat gctgacatct atcgccgagc atgacggtat gccgaaactg ccgtttatca
     2221 gaaagtgaga gaacccgata tgaaatacaa aatcgagaaa aacactgtgc aggaaacgtt
     2281 aattctcccg ttatattccc ggaagctgtg ttcggaactg tacccaaacc tctatcggga
     2341 cgaaacgg
//
LOCUS       DAOMUF010000256         2320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_13149_length_2320_cov_2.426932, whole genome shotgun sequence.
ACCESSION   DAOMUF010000256 DAOMUF010000000
VERSION     DAOMUF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13149_length_2320_cov_2.426932"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..882
                     /locus_tag="PLN77_10235"
     CDS             76..882
                     /locus_tag="PLN77_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM44683.1"
                     /translation="MKMNFAKKATALAMSVLFAVSMAGCGSSASTAKKVEVTSVEDLA
                     DLKIGVQTGTTGDSQASDAVNADSQMQRYNKGADAIQALKNGKIDCVVIDSLPAEKFV
                     AANDDLKIVEGIFDTEEYAMCFKKGNELRDEFNTALAELKEDGTLDEIMSNYIGDEVG
                     QHPYESPADVDRSNGTLTMATNAEFEPWEYKEGTDIVGIDADISQAICDKLGYELKIE
                     DMAFETILASVNSGKADFGAAGMTVTPEREESVDFTDTYANATQVVIVRK"
     gene            976..1665
                     /locus_tag="PLN77_10240"
     CDS             976..1665
                     /locus_tag="PLN77_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM44684.1"
                     /translation="MENLQHQFNHYFVDNGATEWWLTGLKTTLLVTVIALCIGVVLGL
                     LIALIRSTHEQTGKLKLLNIVARVYLTIIRGTPSMIQILFFYSVIFATVNLNNIVIGG
                     IAFGINSGAYVAEIFRSGIMSVDKGQTEAGRSLGLNSAQTMRLIIIPQAFKNVLPALI
                     NEMIVLLKETAIIGYIGTIDITKAATLVQSRTYDALVPLLSAAIFYLILVMILTYFMG
                     KLERRLRKSDH"
     gene            1655..>2320
                     /locus_tag="PLN77_10245"
     CDS             1655..>2320
                     /locus_tag="PLN77_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM44685.1"
                     /translation="MTTNEKVLIKVENLQKSFEDNKVLDGISTEIRQGEVVAVIGPSG
                     SGKSTFLRSLNRLEIPTGGKIYFEGVDITAPETDIDHVRQKIGMVFQQFNLFPHKTVK
                     ENILLAPVTLKLMTPEEAEKKAMELLTRVGLPDKADAYPGSLSGGQKQRIAIARALAM
                     NPDVMLFDEPTSALDPEMVGEVLEIMKELAESGMTMLVVTHEMGFAREVATRVVFINE
                     GNIQ"
BASE COUNT          669 a          498 c          591 g          562 t
ORIGIN      
        1 gctttttaga aagatgctgt tataataagg aaaagtgaat aaatatacat cacatgagga
       61 gagaaggaga ttattatgaa aatgaatttt gcaaaaaaag caacagcact tgcaatgtcc
      121 gttctgtttg ctgtttcgat ggcaggatgc ggaagttctg catctaccgc aaagaaagta
      181 gaagtaacat ccgtagagga tctggcagat ctgaagattg gtgtacagac cggaacaaca
      241 ggtgattctc aggcgagcga tgccgttaat gcagattccc agatgcagcg ttacaacaaa
      301 ggcgccgatg ctattcaggc actgaaaaac ggaaagattg actgtgttgt catcgattcc
      361 ctgccggcag agaaattcgt tgcagcaaac gatgatctga aaattgtaga aggcattttt
      421 gataccgaag agtacgcgat gtgcttcaaa aaaggaaatg agttaagaga cgaatttaac
      481 accgcattgg cagagctgaa agaggacgga actcttgatg agatcatgag caactacatt
      541 ggtgatgaag tcggccagca tccgtatgag tccccggcag acgtggaccg ctccaacgga
      601 acgcttacca tggcaaccaa cgctgagttc gagccgtggg agtacaaaga gggaaccgat
      661 attgttggta tcgacgctga tatttcccag gccatctgcg ataagcttgg ttatgagctg
      721 aaaatcgagg atatggcatt tgaaactatc cttgcatccg tcaacagcgg aaaagcagat
      781 tttggcgcag ccggaatgac tgttactccg gagcgtgagg agagcgttga tttcaccgat
      841 acttatgcaa atgcaacaca ggttgttatt gtaagaaagt aatctaatga gagaaaaaga
      901 gcggctgtcc gccaggcaaa atggtgcagc cgctcttttt gcaggaaatg aaaccacaaa
      961 ggagagatgt attctatgga aaatttacag catcagttta accactactt tgtagacaac
     1021 ggtgctacag agtggtggct gaccggtctg aagacgacgc tgcttgtcac ggtaatcgca
     1081 ctgtgcatcg gtgttgtgct cggacttctc atcgcactga tccgttcaac acacgagcag
     1141 accggtaaat taaaacttct gaatattgtt gcacgcgtct atctgacgat catccgcgga
     1201 acgccgtcca tgattcagat tctgtttttc tattccgtta tttttgcaac cgtaaacctg
     1261 aacaatatcg tgatcggcgg aattgccttt ggtatcaact ccggtgcgta cgttgccgag
     1321 atcttccgtt ccggaatcat gtctgtggat aaaggacaga cggaagcggg acgaagcctt
     1381 ggtttaaatt cggcacagac catgcgtctg attatcattc cgcaggcgtt taaaaatgtt
     1441 cttccggcgc tgatcaacga gatgatcgtg ctgttgaaag agactgccat catcggctat
     1501 atcggaacca tcgatattac aaaagcagca accctggtac agagccgtac atacgatgca
     1561 ctggttccgc ttctctccgc agcgatcttt tatctgattc ttgttatgat tctgacctat
     1621 ttcatgggca aactggaaag gagactgcgt aagagtgacc actaacgaaa aagtattaat
     1681 taaagttgaa aatctgcaga agagcttcga ggataataaa gttttagacg gaatcagcac
     1741 agagatccgt cagggagaag ttgtcgctgt catcggtcct tccggttccg gaaaatcgac
     1801 gtttctgcgc tccttaaacc gtctggaaat tccaaccggc ggaaaaattt atttcgaggg
     1861 tgttgacatc acagcaccgg aaaccgacat tgaccatgtg cgtcagaaga tcggaatggt
     1921 attccagcag ttcaatctgt ttccgcacaa aaccgttaag gaaaatatct tgctcgctcc
     1981 ggtgacgtta aaactaatga caccggaaga agcagagaaa aaagcgatgg agcttctgac
     2041 ccgtgtcgga ctgccggaca aagcagatgc ctatccggga agcctttccg gcggacagaa
     2101 gcagcgaatc gccatcgcac gcgcccttgc gatgaatccg gatgtcatgc tgtttgacga
     2161 accgacttct gcactcgatc cggagatggt aggagaggtt ctggaaatta tgaaagagct
     2221 tgccgaatcg ggcatgacca tgctcgttgt tacacacgag atgggttttg ccagagaggt
     2281 ggcaacccgc gtcgtcttta tcaacgaggg aaatattcag
//
LOCUS       DAOMUF010000257         2318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_13161_length_2318_cov_3.623509, whole genome shotgun sequence.
ACCESSION   DAOMUF010000257 DAOMUF010000000
VERSION     DAOMUF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13161_length_2318_cov_3.623509"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PLN77_10250"
     CDS             complement(<1..884)
                     /locus_tag="PLN77_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44686.1"
                     /translation="MKMKERFHAICRFLTMVSQGNHRVLFWLLGSAFAAATLPYLSLF
                     FSARILNLLLAKSYRACLYTVVVFLLTQYGLGLFEKICRQYLDGQKELCLARTEQKIT
                     AKALELEFEKFEKTETMDAIRRTNVSSMGSGNVGDQLIVIHTLITSLLSVLYALFFLL
                     RLFLLSDSSRNNFFTSSFSMLALLLLCGVQLALSSRINRRSTQKKIELNQGNDHSNSV
                     ANYLVNVMLEERRADDIRIGHLDHFLDVQFGKAMEHFLPMYLDFARFSAITDGKNALL
                     SLLSNFAAYLVIGARALYG"
     gene            complement(884..>2318)
                     /locus_tag="PLN77_10255"
     CDS             complement(884..>2318)
                     /locus_tag="PLN77_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM44687.1"
                     /translation="KAVGATCSNWVGVEGMFRYDVTIYSSFLGLILYAGLIGTVHPAI
                     ILLLLVLSVLQFLSYRRAASYEVRRRDDLSEIEVSQRYFQKQSYNTSAGKDIRLYQLN
                     GWLDGVYRQLNKRYQKILFRIRSAYFANDLLTLTLQLLRDGICYGFLIYRLSHGTMTV
                     SGFVLALGAVSGFSSYFNEITAALSKSVLCLEQIRWLREFFDLPFPAGHTLPFRPELA
                     EEKPLEICFSHVSFSHPGGDKRILDDVSFTLHAGEHTALVGINGAGKSTIVKLICGLY
                     QPTEGNITINGIPLGSLNPAQYYKAIAAVFQDPFALSFSIAENVSCSPLEETDRNRCR
                     EALIRAGLWEKIASLPQKEETYLGKDMADDGITLSGGEMQKLMMARALYKNCHLLLLD
                     EPTAALDAISENKMYETYSTLLKNKTALFISHRLASTRFCDTILFLENGKIKERGTHE
                     ELLALGGSYAHMFEVQSQYYQETEEDI"
BASE COUNT          587 a          564 c          642 g          525 t
ORIGIN      
        1 ccgtacagag ccctggcgcc gatcacaagg tacgcagcga aattggaaag gagcgaaagc
       61 agcgcgtttt ttccgtctgt gatggcggag aagcgggcga agtcgaggta catcggcaga
      121 aagtgttcca tggcttttcc aaactgtacg tcgagaaaat gatcgagatg accgatgcgg
      181 atatcgtccg cgcgccgttc ttcaagcatc acattaacga gataattggc aaccgagttg
      241 gaatggtcgt tgccctggtt cagctctatc tttttctgcg tactcctgcg gttgatgcgg
      301 gaggaaagtg caagctgcac accacagagc aggagcagtg caagcatcga aaacgacgag
      361 gtgaaaaagt tattcctgct gctgtcggac agaagaaaca gacggagcag gaaaaacagg
      421 gcatagagaa ccgagagcag ggaagtgatc agcgtatgaa tgacaatgag ctggtcaccc
      481 acatttcccg atcccatgga ggagacgttg gtgcgccgga tggcatccat cgtctctgtt
      541 ttttcgaatt tctcaaactc cagctccagc gcctttgcgg tgattttctg ttccgtgcgt
      601 gcaagacaga gttctttttg gccgtcgaga tactgacggc agattttttc aaacaatccc
      661 agcccatact gcgtaagcag aaagacgaca acagtataca gacaggcgcg gtacgacttt
      721 gcaagcagta aattcaaaat tcgcgccgag aaaaacaagg agagatacgg cagcgttgca
      781 gctgcaaaag cgcttccgag aagccagaac agcacgcggt gattgccctg gctcaccata
      841 gttaaaaagc ggcagatggc atgaaaccgt tctttcattt tcatcagata tcttcctccg
      901 tttcctgata atactggctc tgtacttcaa acatatgcgc atacgacccg ccgagggcga
      961 gcagctcctc atgggttccg cgctctttga tttttccgtt ttcgagaaac agaatcgtat
     1021 cgcagaaccg tgtcgacgcg aggcggtgcg agatgaaaag cgcagtctta tttttcagca
     1081 gtgtgctgta ggtttcgtac attttatttt ccgagattgc atcgagcgca gccgtcggct
     1141 catcgagcag gagcagatgg caatttttat agagtgcccg cgccatcatc agtttttgca
     1201 tttcaccgcc ggaaagcgta atgccgtcgt ccgccatatc cttgccaaga taggtttctt
     1261 ctttctgcgg cagacttgca attttttccc agagtccggc gcggatgagt gcttctcggc
     1321 aacggttacg atcggtctct tccagtgggg agcagctcac attttctgca atcgaaaacg
     1381 agagtgcaaa cgggtcctga aagaccgccg cgattgcttt gtaatactgt gccggattca
     1441 gactgccaag gggaatgccg ttgatggtga tattcccttc cgttggctga tatagcccgc
     1501 agatcagctt gacaatggtg gattttccgg cgccgttgat gccgacaagt gccgtatgct
     1561 cgcccgcatg gagtgtaaaa ctgacatcat cgaggatcct tttatcgccg cccggatggg
     1621 aaaacgagac gtgggaaaag cagatttcca gcggtttttc ttccgcaagt tccggccgga
     1681 acggcagcgt gtggcctgca gggaacggga gatcgaagaa ttcccgcagc cagcggatct
     1741 gctcgagaca gagcacggat ttggagagcg cggcggtgat ctcgttaaaa tatgaagaaa
     1801 aaccggagac ggcaccgagc gcaagcacaa agccggatac ggtcatcgtg ccgtgggata
     1861 gacggtaaat cagaaagcca tagcagattc cgtcgcgcag aagctgtagt gtaagtgtca
     1921 gcagatcatt tgcaaagtag gcgctgcgga tgcggaacag aattttctga taccgcttgt
     1981 tgagctggcg ataaacacca tcgagccatc cgttcagctg atacagacgg atatcctttc
     2041 cggcggatgt gttgtacgac tgtttctgga aatagcgctg cgatacttca atttcagaca
     2101 gatcgtctct gcggcggact tcgtaagaag ccgcgcggcg gtagctgaga aactgcagaa
     2161 ccgagagcac aagcagcaga agaatgatag ccggatggac ggttccgatc agtcctgcgt
     2221 agagaatcag cccaagaaac gaagaataaa ttgtgacatc gtatcggaac atgccttcca
     2281 cgccgaccca gttgctgcag gtggctccga cggctttt
//
LOCUS       DAOMUF010000258         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_13266_length_2302_cov_3.097463, whole genome shotgun sequence.
ACCESSION   DAOMUF010000258 DAOMUF010000000
VERSION     DAOMUF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13266_length_2302_cov_3.097463"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLN77_10260"
     CDS             <1..209
                     /locus_tag="PLN77_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRM44688.1"
                     /translation="LTDFNQFYVINKICGQEKQRHYLESLGFVPGARISILSELHGYY
                     LVMVKGSKIGLDKSLAKKIIVYAS"
     gene            262..555
                     /locus_tag="PLN77_10265"
     CDS             262..555
                     /locus_tag="PLN77_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44689.1"
                     /translation="MEKNKKIIKLCYLQIAFYVVFLTSLLLHFKRGLDHRAFALLIIL
                     TLLGCASALTLTDYLKSLIRRSGFDVAGVHDKKSLEEKLLQLQNADDALDDDV"
     gene            542..2197
                     /locus_tag="PLN77_10270"
     CDS             542..2197
                     /locus_tag="PLN77_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM44690.1"
                     /translation="MMMFDMNNLKIINDTYGHEEGDVFIQTFASYLTRILTEDSFLAR
                     FGGDEFVIVQNHATWNQLEQMNLQLQTMIDTYNQTADHPLSYAVGYELSCKNHYYLIM
                     DLLQMADEKMYQDKRYKKQLQKNGPLAARRSMLAESISTDSLKEKIFTLLNNRSEEKQ
                     YAFLMTDVDNFHLINDYWGYETGTNILNFILKKLELFPQTLFVNRYHSDIFVGIIDIT
                     GQDPAVVREKISAYHKQAIREVLESYPLNYMTLNTGVYYLNDTDTPAEEVISHANIAR
                     RRAKETSTCVFEYNAELSSTEQKRAETIHSFQNALAKKEFQIYFQPKISGKTQEIASA
                     EVLVRWLREDGTLWFPDSFLPILEETGEIESLDYYVYNAAFQWLADRWENGLPLLPLS
                     LNVSPVHFRKIDTFTQRITQLLDHYQIPSKYLVFEITETTYIHNIDAVNQMIRFFHDR
                     NIRISMDDFGSGYSSLNSLKDILFDEVKIDKHFLSAGLSEKGKIVLEEMFHLLKRTDK
                     WIVCEGVETKEMVDFLIAEGCDELQGFYYYKPMEQKKFEELIA"
BASE COUNT          616 a          554 c          557 g          575 t
ORIGIN      
        1 atttaaccga tttcaatcag ttttacgtca tcaataaaat ctgcggccag gaaaagcagc
       61 gtcattatct ggagtcgctt gggtttgttc ccggggccag gatttcgatt ctgtcggagc
      121 ttcacggtta ttatctcgtc atggtgaagg gcagtaaaat cgggctggat aagagtctgg
      181 ccaagaaaat tattgtgtat gccagctgaa ccgatggcag ggttcacttt ttgcatacgc
      241 atttagaaag tatggagtat tatggaaaag aacaaaaaaa tcatcaaact gtgctatctg
      301 cagatcgcat tctatgtcgt ctttctgaca tcgctgcttc tgcatttcaa gcgtgggctg
      361 gatcatcgcg cctttgcgct tctgattatc cttacgctgc tcggctgcgc ctctgcactg
      421 acgcttaccg attatctgaa aagcctgatc cgccgctccg gctttgatgt cgccggcgtg
      481 cacgataaaa agtcgctgga agaaaagctc ctccagcttc agaacgcgga cgatgcactc
      541 gatgatgatg tttgatatga acaatttaaa gataatcaac gacacgtacg gccacgaaga
      601 aggcgacgtc tttatccaga ccttcgcctc ctatctgacc cgcattctga ccgaggacag
      661 ctttctggcg cggttcggcg gtgatgaatt tgtcattgtg cagaatcatg caacctggaa
      721 ccagctggag cagatgaatc ttcagctgca gacgatgatc gacacctaca accagacggc
      781 ggatcacccg ctgagctatg ccgtcggata tgagctgagc tgcaaaaatc attattatct
      841 gatcatggat ctgctgcaaa tggcggacga aaaaatgtac caggacaagc ggtataaaaa
      901 acagctccag aaaaacggac ctctcgccgc ccgccgcagt atgcttgctg agagcatttc
      961 cacggattct ttaaaagaaa aaatttttac gcttctcaat aacagaagcg aagaaaagca
     1021 gtacgccttt ctgatgacgg atgtcgacaa ttttcatctg atcaacgatt actggggtta
     1081 tgagacgggt acgaacattc tgaatttcat cctgaaaaag ctggaactct ttccgcagac
     1141 actctttgtg aaccgctatc actccgatat ctttgtcggc attatcgata tcaccgggca
     1201 ggatccggcc gtggtgcggg aaaaaatttc cgcctatcac aagcaggcga tccgcgaagt
     1261 gctggaatcg tacccgctga attacatgac gctgaacact ggcgtctatt atctgaatga
     1321 cacggacaca ccagcggagg aagtcatctc tcatgccaac attgcgcggc ggcgtgcgaa
     1381 ggaaacttcc acctgcgtct tcgaatacaa cgccgagctt tccagcacag aacagaagcg
     1441 cgcggagacc attcattcct tccagaatgc ccttgcgaaa aaagaatttc agatttattt
     1501 ccagccgaaa atcagcggaa aaacgcagga aattgcaagc gccgaggtgc tggtacgctg
     1561 gctgcgcgag gacggcacgc tctggttccc ggacagcttt ctgccgattc tggaggagac
     1621 cggagagatt gagtcgctcg actattatgt gtataatgct gctttccagt ggctcgctga
     1681 ccgctgggaa aacggacttc cgctgctgcc gctctcgctg aatgtgtcgc cggttcattt
     1741 ccgcaagatc gatacgttca cacagcggat cacacagctg ctcgatcatt atcagattcc
     1801 ttcgaagtac ctggtttttg agatcacaga aacaacgtac attcataaca ttgatgccgt
     1861 caaccagatg atccgctttt tccacgaccg gaatatccgc atttccatgg acgattttgg
     1921 gtctggatat tcgtccttaa attcgctgaa ggatattctg tttgatgaag ttaaaattga
     1981 caaacatttc ctctcggcgg gcctctccga aaaaggaaaa attgtgctgg aagagatgtt
     2041 ccatctttta aaacgcacag acaaatggat tgtctgcgag ggtgtggaga cgaaagagat
     2101 ggtagatttt ctgatcgccg aaggatgcga cgagctgcag ggattctatt attataaacc
     2161 gatggaacag aaaaaatttg aagagctgat cgcttgacaa agtccgaatt ctatactatg
     2221 gttatctcac ccggtagtta ctaccgagtg aacctcacat gactaaagtc acgtgcttcc
     2281 ggccgcttta ggcggcagac ca
//
LOCUS       DAOMUF010000259         2284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_13412_length_2284_cov_2.526694, whole genome shotgun sequence.
ACCESSION   DAOMUF010000259 DAOMUF010000000
VERSION     DAOMUF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13412_length_2284_cov_2.526694"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1446
                     /locus_tag="PLN77_10275"
     CDS             64..1446
                     /locus_tag="PLN77_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM44691.1"
                     /translation="MTQEKQMTNPLGTDRISTLVARFAVPSIIATLVSAVYNIADQLF
                     IGNAVGTLGNAATNVAFPLSMLCASLALLFGIGGAASFNLHMGAGRKEEAPYYIGNSI
                     TMLLSLGFLLLLITELFLNPLLVLFGSPADVLPLAEQYVRVTAVGFPFFILTIGSGHL
                     IRADGNPKMAMFVTVSGAVINIVLDALFVFGFQWGMYGAAWATVIGQIISAAIAIRYF
                     MHYHTVTLEKQHFIPSGKVLAQICSIGMSSGINQIAVMIVQIVMNNLLKHYGAQSVYG
                     ESIPIACAGIVMKVNQLYFSIVIGLAQGSQPIESFNYGAKQYKRVTDAFLLAASVGAV
                     VSIISFLLFQLFPRQILGLFGSGSEKYFEFGVNYFRAFLFFTWLNFLQPISSMFFSSI
                     GKAYKGTFLSLTRQFLFLLPLFVALPHFFGIMGVLYAGPIADLLSFAVGLSMVIVEFK
                     HINKLEAELA"
     gene            complement(1456..2235)
                     /locus_tag="PLN77_10280"
                     /pseudo
     CDS             complement(1456..2235)
                     /locus_tag="PLN77_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--AMP phosphotransferase"
BASE COUNT          515 a          603 c          487 g          679 t
ORIGIN      
        1 atattataaa tgaaaataca gacattttga cacctgaatt ttataaaaaa ggagtttatc
       61 aacatgacac aggaaaaaca aatgacaaat ccacttggta ccgataggat ttccacgctg
      121 gtggcacgtt ttgctgttcc aagcattatc gcaacgcttg tcagcgcagt ttacaatatc
      181 gcagaccagc tttttatcgg aaacgcagtc ggcacgctcg gaaatgctgc aaccaatgtc
      241 gcctttccgc tctctatgct ctgcgcttcg ctggcactgc tttttgggat cggcggagca
      301 gcaagcttta accttcatat gggcgctgga cgaaaagagg aagcgcctta ttatatcggc
      361 aactccatca ctatgctgct ctcactcgga tttcttttac tgcttatcac cgaactgttt
      421 ttaaatccgc tgttagtgct ctttggttcc ccggcagatg tactcccact ggcagagcaa
      481 tatgtccgcg tcacagcggt cggttttcca ttttttatct taaccatcgg aagcggtcac
      541 ctgatccgtg cggacggcaa cccgaaaatg gcaatgtttg tcacggtttc aggtgctgtc
      601 attaatattg tcttagacgc actgttcgta ttcggtttcc aatggggaat gtacggtgct
      661 gcctgggcga ctgtcatcgg acagatcatc tccgctgcga tcgcgatccg ttacttcatg
      721 cattaccaca ctgtgacatt agaaaaacag cattttatcc cttccgggaa agttctcgca
      781 cagatctgtt ccatcggcat gtcctcaggc attaaccaga ttgccgtgat gatcgtgcag
      841 atcgtcatga acaacctgtt aaaacactac ggcgcacagt ccgtttatgg cgaatccatc
      901 ccgatcgcct gtgccggaat tgtcatgaag gtaaaccagc tttacttttc tattgttatc
      961 ggactggccc agggaagcca gccaattgaa agttttaatt acggtgcaaa gcaatacaaa
     1021 cgggtgactg acgctttcct tctcgcagcg tcagtcggtg cggttgtatc cattatttcc
     1081 tttttgctgt tccagctttt cccgagacag atccttggtc tgttcggaag cggaagcgaa
     1141 aaatattttg agttcggtgt caactatttc cgagccttcc tgtttttcac ctggttaaac
     1201 tttttgcagc cgatctcctc gatgtttttc agttccatcg gaaaagcgta caagggaact
     1261 ttcctctccc tgaccagaca gttcctgttc ctgctgccgc ttttcgttgc gctaccgcat
     1321 tttttcggca ttatgggcgt actttatgca ggaccgattg ccgaccttct gtcctttgca
     1381 gtcggtctta gcatggtgat cgttgaattc aaacatatta ataagctgga agctgagctg
     1441 gcttaatatt atcctttacc ccaacacctt ctccagctcc tccacaacga tcttaagagc
     1501 tttgatccgg gcatatttct tgtcgacaga ctctaaaata tgccacggag cataggaggt
     1561 gctggtcttt tggatcatct cattgaccgc tgtctcatac gcatcccatt tgtcacggtt
     1621 gcgccagtcc tcgtctgtga tcttccactg tttttccggt gtattctgtc tctcattaaa
     1681 acgctcaagc tgcgtatctt tgtcaatctg cacccagaat ttgatgatca cagcgcccca
     1741 gtcgtgaagt tctttttcaa attcgttgat ctcattgtag gcgcgcatcc agtcattttc
     1801 actgcaaaat ccctccagac gctccaccat cactcttccg taccaggtgc ggtcaaaaat
     1861 agcgatatgt cctgttttcg gcagtctcgt ccagaaacgc cacaggtaat gtcttgcttt
     1921 ctcatgaggt tccgggcttg cgatcggatg aacttcgtaa ccgcgcgggt cgagcgctcc
     1981 tgcgatccgc ttgatattgc cgccttttcc tgctgcatcc cagccctcat aggcaatgat
     2041 gaccggcact tttttccggt aaagacgatt gtgcagctca ccgagccggt tctgcaattc
     2101 tttcagttct ttgcggtact cgtccgtctc aatctctttg tctaacggca cttctgacaa
     2161 aagcggcatt gggactaacg ggaaaacatt ctgcaaaaga ggcactgcca gtttctgatt
     2221 actcaacgca atatcccctt gtttcccgcc agcctttcac ataccatctt atcacgacat
     2281 atat
//
LOCUS       DAOMUF010000260         2247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_13699_length_2247_cov_2.281478, whole genome shotgun sequence.
ACCESSION   DAOMUF010000260 DAOMUF010000000
VERSION     DAOMUF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13699_length_2247_cov_2.281478"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PLN77_10285"
     CDS             complement(<1..1083)
                     /locus_tag="PLN77_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRM44692.1"
                     /translation="MAVKWTAEQQKVIDTRDRNLLVSAAAGSGKTAVLVERILAMVKD
                     PVRPIDIDQLLVVTFTRAAAGEMKERIGRALQQSLEENPENEHLQRQQTLLHHAQIST
                     IHGFCSYVIKNYFQMIDLDPVYRMADEGEIRLMKSDVIQKVMEERFAEKDPAFTRFVE
                     SYAAGKQDDGLDALVLKLYEFSMSYPWPEQWLEECRAAYQIDSPEAVQKSIWVQVLMA
                     EAKKRLSSILEGIEENRKTAVSPGGPYLYDEALASDQQMVEELIETESFDGMVRAMAG
                     MKFKALSRKKADDVSEALKDQVKAERDACKKELQKLKEQFFEKDSAEIAEEIADCREP
                     IGVLIDLTEQFAKAFAEKKRSKNILDY"
     gene            complement(1156..>2247)
                     /locus_tag="PLN77_10290"
     CDS             complement(1156..>2247)
                     /locus_tag="PLN77_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRM44693.1"
                     /translation="FMEFGLQIRERDQYELKAVDMGTVMHEALEKFSKKLQENGETWK
                     TVGDDTRDRLIEECVEETMADYGNTIFQSSSRNQYRIIRVKRILKRTVWALQQQIRQG
                     EFEPGEFEVSFSMEDSLSAINIDLSEHEKMRLRGRIDRVDLCETDDKVYVKIIDYKTG
                     NTSLDLVALYYGLQLQLAVYLDAAVELEQKKHPGKLVEPAGVFYYHIDDPILDQEEDE
                     TDEAWGRRMLKALRMDGLVNADRKVVELLDRVLEDGTTSDVIPVGKKKDGSYTSYSKV
                     ASPEQFDVIRTYTRKKVREIGEGIFSGNVKISPYQRDGATACAYCEYQGICGFDQKIQ
                     GYEYRKLKGMDTELLMKAMQEITQSDQKE"
BASE COUNT          459 a          733 c          433 g          622 t
ORIGIN      
        1 ataatcaaga atatttttgc tccgtttttt ctctgcaaac gctttcgcaa actgctccgt
       61 cagatcgatc agcacgccga tcggctcccg gcagtccgca atctcctccg caatctcggc
      121 gctgtccttc tcgaaaaact gctcttttaa tttctgcagt tcttttttgc atgcatcgcg
      181 ctctgccttc acctgatctt tcagcgcctc gctcacatcg tctgcctttt tccgcgaaag
      241 cgccttgaac ttcattccgg ccatcgcgcg caccattccg tcgaacgatt ccgtctcgat
      301 cagctcttcc accatctgct gatctgatgc cagcgcctcg tcatacagat acggtccgcc
      361 agggcttacc gccgtttttc ggttttcctc aattccttcc agaatactcg agagccgctt
      421 tttcgcctca gccatcagta cctgtaccca gatgcttttc tgcaccgcct ccggcgaatc
      481 gatctgatac gccgcccggc actcttcgag ccactgctcc ggccacggat aactcatgga
      541 aaattcatac agcttcagca cgagcgcgtc gagtccgtca tcctgttttc ccgccgcgta
      601 gctctccaca aaccgtgtaa acgcaggatc tttttccgca aaccgctcct ccatcacctt
      661 ctggatcacg tcacttttca tcaggcggat ctctccctca tccgccatgc ggtataccgg
      721 atcaagatcg atcatctgaa aataattttt tatcacatac gagcagaagc catggatcgt
      781 gctgatctgt gcatggtgaa gcagggtctg ctgccgctgt aaatgctcat tctctggatt
      841 ttcctcgagg ctctgctgca gggcgcggcc gattctctct ttcatctctc ccgccgctgc
      901 acgcgtaaag gtcacgacaa gaagctgatc aatatcaatc gggcgcacgg gatctttcac
      961 catcgcaagg atccgctcta ccagaaccgc cgtctttccg gaacctgccg ctgcgctgac
     1021 caaaagattc cggtctctcg tatcaatgac tttctgctgt tccgctgtcc attttactgc
     1081 catttatctc tccctcttct ttctggctga ttactttctc atacatggaa aactgcattt
     1141 ctctttcctt tccagtcact ccttttgatc actttgtgtt atctcctgca tcgccttcat
     1201 cagcagttcc gtgtccatgc ccttcagttt gcggtactcg tatccctgaa ttttctggtc
     1261 aaaaccgcag attccctgat attcacagta cgcacacgcc gtcgcaccgt cccgctgata
     1321 cggggaaatt tttacattgc ccgaaaaaat gccctcgccg atctcacgca ccttctttct
     1381 ggtgtacgta cggatcacat caaactgctc cggcgatgct actttcgaat agctcgtata
     1441 acttccatcc ttcttctttc caaccggaat cacatccgac gtcgttccgt cctcgagtac
     1501 acggtcgaga agctccacca ccttccgatc tgcatttaca aggccatcca tccgcagcgc
     1561 tttcagcatc cgtctgcccc acgcctcatc ggtctcatct tcttcttggt cgaggatcgg
     1621 atcatcaata tgataataaa atacccccgc aggctccacc agcttacctg gatgcttttt
     1681 ctgctccagc tccaccgccg catccagata aaccgccagc tgcagctgta acccataata
     1741 gagcgccacc aggtcaagcg atgtatttcc cgtcttgtaa tcgataattt tcacatagac
     1801 cttatcgtcc gtctcacaga gatccacgcg gtcaatccgt ccgcgcaggc gcatcttctc
     1861 atgctccgac agatcaatat taatcgccga caaggagtcc tccatggaga acgaaacctc
     1921 aaattcaccc ggctcaaatt ctccctgccg aatctgctgc tgcagcgccc acaccgtccg
     1981 tttcagaatc cgctttacgc ggatgatgcg atactgattc cggcttgagc tctggaaaat
     2041 cgtgtttccg taatctgcca tcgtctcctc gacgcactcc tcgatcaggc ggtcacgggt
     2101 atcatccccg acagtcttcc atgtctcgcc attttcctgc agtttttttg aaaacttttc
     2161 gagtgcttca tgcataaccg ttcccatatc gaccgctttc agctcatact gatcccgctc
     2221 gcggatctga aggccgaact ccataaa
//
LOCUS       DAOMUF010000261         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_13714_length_2245_cov_4.049315, whole genome shotgun sequence.
ACCESSION   DAOMUF010000261 DAOMUF010000000
VERSION     DAOMUF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13714_length_2245_cov_4.049315"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLN77_10295"
     CDS             complement(<1..532)
                     /locus_tag="PLN77_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRM44694.1"
                     /translation="MIRFILVILTAFLYLILSIPVLLVLLLIRKKKPEVCDKVSRALI
                     RWIFRVILFFAGTKITVEGQENIPKDRAVLYIGNHRSYFDILVTYTTVPGSCGFVAKK
                     ELARIPLLKNWMDLIHCLFLDRSDIKQGLQMILAGCEEIKSGTSICIFPEGTRGKTPS
                     ELELGEFHNGSFKMATK"
     gene            710..1132
                     /locus_tag="PLN77_10300"
     CDS             710..1132
                     /locus_tag="PLN77_10300"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HRM44695.1"
                     /translation="MMKKKRRGRVLLAAVILMLLASCRITDVSEGERKELSYAIVKPG
                     DFPPEIDQILRRKKESAFQMAYESGDDLYILRGYGKQKSGGFSIQIEEVSKSENAVFV
                     RTKLVGPAAKEEQKGAASCPYVVLKTKAAAGISVVFED"
     gene            1148..>2245
                     /locus_tag="PLN77_10305"
     CDS             1148..>2245
                     /locus_tag="PLN77_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HRM44696.1"
                     /translation="MDFVKKILHQISRKSEAVSQITFDEDYNVPDARPDVGRMIQKKG
                     EVTIGDVQISEGKARVFGGLTFHLLYVSDGERRRICQLSGELPIDETIHLDGLTGGDK
                     VCITWEVEDLNLHLINSRKLGVRAIVTLHAWIEELCDLAVPMEIRGESDVAVKRQEYR
                     VVELAVQKKDVLRVKKELTIPSGKPELHEILWQDLEVRGLDLRSEEGRVSAQGELFVF
                     CLYSDGEEDHPLQWVEQALPFQAEVECQGCISEMIPRIESTLGQTTLEIQPDSDGEER
                     VLQAEAVLELDICLYQEETVSLLQDVYHPHRECIPKVQEETLESLLIRNSSKCRLTDK
                     LQMRVPKNKILQICHSDGKIKIDEERIVENGI"
BASE COUNT          579 a          539 c          660 g          467 t
ORIGIN      
        1 ttttcgttgc catcttgaat gaaccgttgt ggaactcgcc gagctccagc tcgcttggtg
       61 ttttgccgcg ggtgccctcc gggaagatac agatggaagt gccgctcttg atctcctcac
      121 atccggcaag gatcatctgc agaccctgtt tgatgtcgga gcggtcgaga aacaggcagt
      181 ggataagatc catccagttc tttaagagcg gaatgcgcgc cagctccttt ttcgctacga
      241 atccgcagga acccggaacc gttgtgtagg ttacgagaat atcaaaatag ctgcggtgat
      301 tgccgatgta gagaacggca cgatctttcg gaatattttc ctgtccttcc accgtgatct
      361 tcgtgccggc gaaaaacaga atcacgcgga aaatccagcg gatcagcgca cggcttacct
      421 tgtcacagac ctccggcttt ttcttccgga tcagaagcag aacaagaagc accgggatcg
      481 acagaatcag atacagaaaa gcggtcagaa tgaccagaat aaaacgaatc atgaatcttt
      541 ttctcctctt ggtaaaaata gtgatattca gttattgcgt acttttcagt atacgtcata
      601 tatggggcgg gttgcaataa ctttcgtctt tttcggaaaa agattttgcg tcatagaaga
      661 ctgtggcccg tcatacaatg gatagaaaga gcaggaagcg gcgtttggaa tgatgaagaa
      721 aaagagacgg ggaagagttc ttctggcggc ggtgatcctg atgctcctgg catcgtgccg
      781 gatcacagat gtgagcgagg gcgagcggaa agaactgtcg tatgcgattg taaagccggg
      841 cgattttccg ccggaaatcg atcagatttt gcgcaggaaa aaagaatccg cgttccagat
      901 ggcgtacgag agcggggacg atctctacat actgcgcggc tacggaaagc agaaaagcgg
      961 aggcttttcc atacagatcg aagaagtatc gaaatcggaa aacgccgttt tcgtgcggac
     1021 aaaactcgtc ggacctgccg caaaggagga gcagaaaggc gccgcctcct gcccttacgt
     1081 cgtgctgaaa acgaaagcgg cggcgggaat ctcagtcgta tttgaggatt agagtgggag
     1141 gagccttatg gattttgtaa aaaagattct gcatcagatc agcagaaaaa gcgaagcggt
     1201 gtcacagatt acgtttgatg aggactacaa tgtgccggac gcacggcccg atgtggggcg
     1261 gatgattcag aaaaaaggcg aggtgacgat cggggatgtg cagatttcgg aaggaaaagc
     1321 gcgtgtattc ggcggcctca cctttcatct gctgtacgtc tccgacggcg aacggcgccg
     1381 gatctgccag ctctccggcg agcttccgat cgacgagacg atccatctcg atggtctgac
     1441 agggggcgac aaggtctgca tcacctggga agtggaagat ctgaacctgc atctcatcaa
     1501 ctcgcggaag ctgggcgtgc gcgcgatcgt cacgctgcac gcgtggatcg aggaactgtg
     1561 cgaccttgcc gtgccgatgg aaatccgcgg ggagagcgac gtcgcggtaa aaaggcagga
     1621 ataccgcgtg gttgagcttg cagtgcagaa aaaagatgtg ctgcgtgtca aaaaggagct
     1681 tacgattccg tccggcaagc cggaattgca cgagattctc tggcaggatc tggaagtgcg
     1741 cggtctcgat ctccgctctg aggaggggcg ggtcagtgcg cagggagaac tctttgtctt
     1801 ctgtctgtac agcgacggcg aggaggatca cccgctgcag tgggtggagc aggcgctccc
     1861 gttccaggca gaagtggaat gccagggctg catctccgag atgatcccgc ggatcgaaag
     1921 cacactcggc cagaccacac tcgaaatcca gcccgattcc gacggcgagg agcgggtcct
     1981 gcaggcggag gccgtactcg aacttgacat ctgtctgtat caggaagaaa ccgtatcact
     2041 tctgcaggac gtttaccatc cgcaccgaga atgcattcca aaagtgcagg aggagacttt
     2101 ggagagcctt ctgatccgca attcctccaa atgccgcctg accgacaaac tgcagatgcg
     2161 cgtgccgaaa aataaaattc tgcagatctg ccacagtgac ggcaaaatca agatcgacga
     2221 ggaaagaatc gtagaaaacg gcatc
//
LOCUS       DAOMUF010000262         2206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14053_length_2206_cov_2.578801, whole genome shotgun sequence.
ACCESSION   DAOMUF010000262 DAOMUF010000000
VERSION     DAOMUF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14053_length_2206_cov_2.578801"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PLN77_10310"
     CDS             complement(<1..144)
                     /locus_tag="PLN77_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM44697.1"
                     /translation="MISEKIFELILRNGMTQKEFSEETGISQSTISDWKRKGTNPSAD
                     KILK"
     gene            complement(205..1059)
                     /locus_tag="PLN77_10315"
     CDS             complement(205..1059)
                     /locus_tag="PLN77_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM44698.1"
                     /translation="MNIEYLKYFAELAKLQHYGKAAKALNISQPGLSHAIKTLEDEYG
                     VPLFQKEGRNVSLSRYGKELMQDVEEILDAYLRMEERAAGLRTEEKTVRIGTVYPLAA
                     GTIPHMLREFGATFPFIIYNRLTPEIEEGLLDGKYDIGFCSELLKSEELEYYPLRESY
                     IAAAVPEGHPLEGKENITLKEAAKYPQVMFSRTSGFRSLQEQIFAEQGIQVKSVCEAE
                     EIEVITGLVENGFGISILPYMDIVRLHHITAIPVQTSSWKSKFYIARRKYGIRTAQEE
                     AFFQHWRK"
     gene            1226..>1679
                     /locus_tag="PLN77_10320"
     CDS             1226..>1679
                     /locus_tag="PLN77_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HRM44699.1"
                     /translation="MNTRKFHLPGEAALLIVLLINPLAIDLMSKSVFGISTISSVPLI
                     LSTAFPVFSFGTWNYIFQTLLVITLMVLKRSFCPGYLFSFVVGIGFGKMIDVHNTWVT
                     LLPNVLSLNVVCFFTGFLLMCFGICLANNCLLPIVPTDIFPRDLSELLH"
     assembly_gap    1680..1689
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1690..1927
                     /locus_tag="PLN77_10325"
     CDS             <1690..1927
                     /locus_tag="PLN77_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HRM44700.1"
                     /translation="KKYNQIKTTFDLTCLTTTVILSLVILHHLYGIGIGTFFCAFMTG
                     RTVSLVQKFIGNHVEFYRLMKKEMVMPGKKMRHA"
     gene            complement(1975..>2206)
                     /locus_tag="PLN77_10330"
     CDS             complement(1975..>2206)
                     /locus_tag="PLN77_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRM44701.1"
                     /translation="AAALMRPSKSTKDMELKSLKKKYKSKGFAAGCSREVIERGAEQL
                     DWPLEKLLTMTMQAMADSEDEINREVEAEQNA"
BASE COUNT          468 a          563 c          465 g          700 t
ORIGIN      
        1 tttcagtatt ttatccgccg acggattcgt gccctttctt ttccagtcgc taatggtgct
       61 ctgggaaatt ccggtttctt cggagaattc tttctgggtc atgccgtttc gaagaattag
      121 ctcaaaaatt ttttcactga tcattttgta tctcaccttt ttcacttaga ttttgtttta
      181 ctataccata aaatttcctg cccgtcattt cctccaatgc tgaaaaaacg cctcttcctg
      241 cgctgtgcgg attccgtatt ttcttctggc gatgtagaat tttgatttcc agcttgaggt
      301 ctgcaccggg attgcggtga tgtgatggag gcggacgatg tccatgtagg ggaggataga
      361 tattccaaaa ccattttcca caaggccggt gatgacttcg atttcttcgg cctcacacac
      421 ggattttacc tggatgccct gttcggcgaa gatctgctct tggaggctgc ggaagccgct
      481 cgtcctggaa aacatgacct gcggatactt ggctgcttct tttagtgtga tgttctcttt
      541 cccctccagc ggatgacctt ccgggacggc ggccgcgatg taagattccc ggaggggata
      601 atattcgagt tcttcggatt tcagcagttc tgagcagaag ccgatatcgt attttccatc
      661 cagcagaccc tcctcgatct ctggggtcag gcggttgtag atgataaacg ggaaggtcgc
      721 gccgaattcc cgcagcatat gtggtattgt tcctgccgcg agcggatata cggttccgat
      781 ccgcactgtc ttttcttccg tccgaaggcc tgccgcccgc tcctccattc gaagatatgc
      841 gtccagaatc tcctcgacat cctgcatcaa ttcttttccg tagcggctca ggctcacgtt
      901 tcgtccttct ttctggaaga gcggaacacc gtactcgtcc tccagtgtct tgatcgcatg
      961 gctgagcccc ggctgggaaa tattcagcgc ttttgccgct tttccatagt gctgcagttt
     1021 tgccagctct gcaaaatatt ttaaatactc gatattcata gatagcctct tttcctgttc
     1081 tgcatctatc ataccacttt tcatttaatt ttagaatcaa ttcattcaaa ataatcattt
     1141 gtttttatcg gtttctgtgt ctataataag catagtttca agaccttaac gtaatttcat
     1201 tctcaggaaa gaaggatttt tactcatgaa taccagaaaa tttcatcttc caggcgaagc
     1261 ggcgcttctg atcgtgcttc tgatcaaccc tcttgccatt gatctgatgt caaaaagcgt
     1321 atttggaatt tcaacaattt cctccgtgcc gctcatcctc agcacagcgt ttccagtttt
     1381 cagtttcgga acgtggaact atatctttca aacgctgctt gtcatcaccc tgatggtcct
     1441 gaagcgttcg ttctgtcccg gctatctgtt ttcttttgtt gtcggcatcg gattcggaaa
     1501 aatgatcgat gtacacaata cctgggttac cctgctgcca aatgttctgt cgctgaatgt
     1561 ggtctgcttt ttcaccggat ttcttctgat gtgcttcggc atctgcctcg ccaacaactg
     1621 cctgctgccg attgtcccga ccgatatttt tccgcgcgat ctgtctgaac tgctacacan
     1681 nnnnnnnnnt aaaaagtata accagatcaa gacaaccttt gatctcacct gtctgacaac
     1741 gaccgtaatc ctgtcccttg taatccttca ccatctttac ggaatcggca tcggaacttt
     1801 cttctgtgct tttatgacgg gacgaaccgt atctctggtt caaaaattca ttggaaatca
     1861 tgtagaattt taccgtctga tgaaaaaaga aatggtaatg cctgggaaaa agatgcgcca
     1921 tgcataaaaa aactgcccga attccagttt ttctgttgaa attcgggcag ttttttatgc
     1981 gttctgctcc gcttctactt cccggttgat ctcatcctcg ctgtcagcca tggcctgcat
     2041 tgtcatagtc agcagttttt ccagcggcca gtcaagctgc tcggcgcccc gctcaatgac
     2101 ttcccgcgaa catccagccg caaagccttt gctcttatat tttttcttca gggatttcag
     2161 ttccatgtct tttgtgctct tcgaaggccg catcagcgca gctgcc
//
LOCUS       DAOMUF010000263         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14234_length_2185_cov_2.110798, whole genome shotgun sequence.
ACCESSION   DAOMUF010000263 DAOMUF010000000
VERSION     DAOMUF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14234_length_2185_cov_2.110798"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PLN77_10335"
     CDS             complement(<1..960)
                     /locus_tag="PLN77_10335"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRM44702.1"
                     /translation="MAEKVRDLRSALERLRNMPGQLAETDVEVDPMAELAGVYRYVGA
                     GGTVKRPTKEGPAMIFRNVKGHPDASVAIGVLASRKRVAALLDCEPESLGKMLYHSVE
                     EPIAPVEFQGAPPCQEVVHLATDPDFDLNRLVPAPTNTPDDAGPYITLGMCYASHPDT
                     GLHDVTIHRLCIQGKDELSIFFTPGARHIGAMADRAEELGQTLPISISIGVDPAIEIG
                     SCFEPPTTPLGYDELSVAGALRKEPVELCRCVSVKEMAIANAEYVIEGEVIPGVRVQE
                     DQNSHTGYAMPEFPGYTGPASDQCRLIKVKAVTHRRSPIMQTCI"
     gene            1334..1984
                     /locus_tag="PLN77_10340"
     CDS             1334..1984
                     /locus_tag="PLN77_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HRM44703.1"
                     /translation="MLQKMHHFQETTRQPLRVGSLPFLTQYHLTSRIRAFTHAHLEIE
                     LTLEECEETELMDGLQSGHFDLVIARDSMISLQKYHFEPITEDRLCVMLPIDHPLAEK
                     PALTIADLAAEPLILMHPYTSIYQLCMQLFEKAGVKPQILRTARLESTISSVEIGEAF
                     SLFAESNFELFRHPNVTAVPLLDAPPLVIGAAYPLRTETMKKPALKSFSSFIQDPT"
BASE COUNT          491 a          666 c          502 g          526 t
ORIGIN      
        1 gatgcaggtc tgcataatcg gactgcggcg gtgtgtcacg gcttttactt taatcagacg
       61 gcactgatca cttgccgggc cggtgtagcc cgggaactcc ggcatcgcat agccagtgtg
      121 gctgttctgg tcctcctgca cacgcacacc cgggatgacc tcgccctcga tgacgtactc
      181 tgcatttgca atcgccatct ccttcacgga aacgcagcgg cacagttcta ccggctcttt
      241 ccgcagtgca cccgcaacgg aaagctcatc gtaaccgagc ggtgttgtcg gcggctcgaa
      301 acaggaaccg atctcaatcg caggatcaac accgatactg atggaaatcg gaagtgtctg
      361 tcccagttcc tccgcgcggt ctgccattgc gccgatgtgg cgtgcgcccg gggtgaagaa
      421 aatcgaaagc tcgtcttttc cctgaatgca gagacggtga atcgtcacat catgcagtcc
      481 ggtgtccgga tgggaggcgt agcacatgcc gagcgtgatg tacgggccgg catcatccgg
      541 cgtattcgtc ggcgccggga cgagacggtt cagatcaaaa tcgggatcgg tcgccagatg
      601 taccacttcc tggcatggcg gcgcgccctg aaattcaacc ggtgcaatcg gctcttctac
      661 gctgtgatac agcatttttc caagactctc cggctcgcag tccagcaggg cagccacgcg
      721 tttccggctt gccagaacac caatcgcaac gctggcgtcc gggtgccctt ttacgttgcg
      781 aaaaatcatc gccgggcctt ctttcgtcgg acgctttacg gtcccgcccg caccgacgta
      841 acggtaaaca ccggcaagct ccgccatcgg atcaacctca acgtctgtct cggcaagctg
      901 tcccggcata tttctcaatc gttccagcgc actacgcaga tctcttactt tttcagccat
      961 agttcttctc cttttattct atcgacggcg ccccgaagga tgccttttca tcctcatttt
     1021 ctacccttat tgtaaaaaga aacctttgtg gaatcaattc gatttgtggt acaattgttt
     1081 ccagaatgga atgaatcaaa cgaaaaggag gctcccctta tgactctgga acagatcgac
     1141 ttttttctga tggccgttcg atgcgacacc tttttagagg ccgcggaaaa tcttcatatc
     1201 gcacagtcca cgctctcgaa acagatccag aagctcgaga ctgaattgaa tcttcccctc
     1261 ttcgaccgca cccgccgcca ggccgtactg actccggcag gaagccgcgg agctctccct
     1321 ccagtatcac cagatgctgc aaaaaatgca tcacttccag gaaaccacgc gccagccgct
     1381 ccgcgttggc tctcttccgt ttctgacgca gtatcatctc acttcccgca tccgtgcctt
     1441 cacgcacgcg catctggaaa ttgagctgac tctggaagaa tgcgaagaga ctgagctgat
     1501 ggacggcctg caatcaggcc attttgacct tgtgatcgcg cgggattcca tgatttcact
     1561 gcagaaatac cacttcgaac ccatcacaga agaccgcctc tgcgtgatgc tgcccatcga
     1621 tcatccactt gccgaaaagc ctgctcttac catcgctgat cttgccgccg aaccgctgat
     1681 tctcatgcac ccctatacct cgatttatca gctctgcatg cagctatttg aaaaggcagg
     1741 tgtaaaaccg cagatcctgc gcacggcccg cctggaatcc acgatcagct ctgtggaaat
     1801 cggcgaggct ttcagtctct ttgccgagag caactttgaa cttttccgcc atccaaatgt
     1861 caccgccgtt cctcttctcg acgcaccacc gcttgtaatc ggtgcagcgt atccgctccg
     1921 aacggaaaca atgaaaaaac cagcattaaa atctttttcc tctttcattc aagatcccac
     1981 ataaccaaaa ctcgcttcac tcgttatcgc ggcattcgtc aaggtctcat gcaagcacgg
     2041 acttttcctc attgcacgcg aaaaatacgc ccctgacact ttgaactgtg ccagaggcgt
     2101 acgttttatt tactataacc tgttttccaa ttactgaact gccttaaatg cctcatccag
     2161 atcctcgata atatcatcga cattt
//
LOCUS       DAOMUF010000264         2181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14263_length_2181_cov_2.711195, whole genome shotgun sequence.
ACCESSION   DAOMUF010000264 DAOMUF010000000
VERSION     DAOMUF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14263_length_2181_cov_2.711195"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PLN77_10345"
     CDS             complement(<1..1192)
                     /locus_tag="PLN77_10345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRM44704.1"
                     /translation="MDLTDIIYSNDYINILIQNYVAQSERYQMLFPESSALLTLNPQY
                     SVLFLERSLLPENLFGAVPFTGLPKLFTPLSTLSLEVSGIVQTQNLYGGGYTGKNVIF
                     GFLDTGIDYRHPAFLHANGQSRILAVWDQTDRTGTPPAQFPYGSLYTKSDLDAALESS
                     DPLSLVPVTDPDGHGTYVAGVAGGTPDASAGFLGVAPEADFAVVKLKPAKQNLKDFYL
                     VSSNAPVFEEDDLLSAMHFLNETAAAEGKPLVLCLALGTNQGSHSGTLPVSLTLARYG
                     NTPGVIPVCATGNEGNASHHYYGSVSETYTPKNVEFLVSEGNRGFTLELWGQAPQLFS
                     VGFRSPGGETIPRIPVSLNQEQRITFVLERTVIYVNYEVVQATTGSQLILIRMLDPTP
                     GIWTL"
     gene            complement(1310..2181)
                     /locus_tag="PLN77_10350"
                     /pseudo
     CDS             complement(1310..2181)
                     /locus_tag="PLN77_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331258.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; frameshifted; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
BASE COUNT          509 a          525 c          651 g          496 t
ORIGIN      
        1 gaagtgtcca gatgccgggc gtcggatcca gcatgcggat cagaatgagc tggctgccgg
       61 tcgtggcctg gaccacttcg tagttgacgt agatcacggt gcgctccaga acgaaggtga
      121 tccgctgttc ctgattcaaa gagacgggaa tccgcggaat cgtctcgccg ccgggagagc
      181 ggaaaccgac agaaaaaagc tgtggagcct gtccccagag ttccagggtg aaaccgcggt
      241 ttccttcaga aacgagaaac tctacatttt tcggggtgta agtttcggat acagagccat
      301 aataatggtg tgatgcattt ccttcgttgc cggtcgcgca gacggggatc acaccaggcg
      361 tgttgccgta gcgcgccaag gtcagagaaa cggggagcgt gccggagtgg ctgccctgat
      421 tcgtcccgag tgccagacag aggacgagcg gttttccttc cgcagctgcg gtttcattca
      481 gaaagtgcat ggcggagagc aggtcatctt cctcgaagac gggagcattg gaggacacaa
      541 gataaaaatc tttcagattc tgtttggcgg gttttaattt aacaacggca aaatctgcct
      601 ccggtgcgac gccgagaaat ccggcggagg catccggagt accgccggcc acgcctgcga
      661 catacgtgcc gtgcccgtcg ggatcggtga ctggaacaag ggagagtgga tcgctgcttt
      721 caagtgccgc gtcgagatcg gacttggtat agagactgcc gtaaggaaac tgcgccggcg
      781 gcgttcccgt ccggtccgtc tgatcccaga cggcgagaat ccggctctgt ccgttggcat
      841 gcagaaatgc ggggtggcgg taatcaatgc cggtgtccag aaaaccgaag attacatttt
      901 tgcctgtata tccgccgccg tagagattct gcgtctggac aatcccggag acttccagtg
      961 agagcgtgga gagcggcgta aaaagttttg gaagcccggt aaagggaact gcgccgaaga
     1021 gattttccgg cagcagactc cgctcgagaa agagaacgga atactgcggg ttcagcgtaa
     1081 gaagagcact tgattccgga aacagcatct ggtaccgctc ggactgtgcc acgtaattct
     1141 ggatcagaat gttgatgtaa tcgttggaat aaatgatgtc tgtaagatcc ataaagagat
     1201 cctttgtttt tgatgctatt gtatgcaaac attacgtcaa agtatgcatg aaacaaaaaa
     1261 gcccccttac ctgacgcagt gtctgcgcgg taagggggac gaaatagaat cagctttcga
     1321 gaagagcgct gatatcctgt ttcggtactt ccattgggta atggctgtcg aagcatgcac
     1381 ggcagatcgg cagatcgccg accatgttct ggagttcctc gatcggcatg tagccaagag
     1441 agtcggctcc gatctgctgg cggatctcct ccgtggagtg agagcttgcg atcagctgtt
     1501 tgttcgaagg aacatcggtg ccgaaatagc acggatacag gaacggcgga gagctgatac
     1561 ggacatggac ttcggtcgcg ccggctttgc gatcgtgatc aggttggaaa tcgttgttcc
     1621 acggacaatg gagtcgtcga cgagcacgat ccgttttcct tttacaacag attcgagaac
     1681 gctcagcttg attttgacgc tggattcccg ttccttctgg gtcggcttga tgaaggtacg
     1741 gccgacatag ctgtttttgt ggaacgcaag accgaacggg atgccggatt cttcggaata
     1801 gccctttgcg gaggcaagtc cggagtccgg gacaccacag acgaggtctg catcaaccgg
     1861 gtaggtcttt gcgagagttg caccgccgcg gattctggct tcgtagacac tgatattgtc
     1921 tattttgctg tcgaggcggg caaaataaat atattcaaaa atacaatgcg ctttttttac
     1981 gtcaaccggc ataaattcgg atttcagatt tccggttttg tcacacgtta caatttctcc
     2041 cggagcgaca tcgcggacga attcggcgcc gatcgaagtg agggcgcagc tttccgaggc
     2101 gagaacccag gcgttgcccc gctttccgat gcagagcggt tttaagccat atgggtcacg
     2161 cacgccgatg agttttctag g
//
LOCUS       DAOMUF010000265         2165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14414_length_2165_cov_3.713270, whole genome shotgun sequence.
ACCESSION   DAOMUF010000265 DAOMUF010000000
VERSION     DAOMUF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14414_length_2165_cov_3.713270"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="PLN77_10355"
     CDS             complement(<1..1353)
                     /locus_tag="PLN77_10355"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRM44705.1"
                     /translation="MYKSFSMELAGRTLTVDVGRVAKQANGAAFMHYGDTVVLSTATA
                     SEKPRDGIDFFPLSVEYEEKMYAVGKIPGGFNKREGKASEHAILTSRVIDRPMRPLFP
                     KDYRNDVTLNNMVMSVDPECDPEVVAMLGSAIATCISDIPFDGPCAMTQIGMIDGEFI
                     VNPTLAQKAVSDLKLTVASTREKVIMIEAGAKEIPEAKMIDAIYKAHEVNQEIIKFID
                     SIVAEVGKPKHAYESCAIPEELFAAIKEIVPPAEMEEAVFSDDKQTREENIRVITEKL
                     EEAFADNEEWLAVLGEAVYQYQKKTVRKMILKDHKRPDGRAITEIRPLAAEVDIIPRV
                     HGSAMFTRGQTQICNVTTLAPLSEAQKLDGLDEFETSKRYMHQYNFPSYSVGETKPSR
                     GPGRREIGHGALAERALVPVLPTEEEFPYAIRTVSETFESNGSTSQASICASTMSLMA
                     A"
     gene            complement(1606..1872)
                     /gene="rpsO"
                     /locus_tag="PLN77_10360"
     CDS             complement(1606..1872)
                     /gene="rpsO"
                     /locus_tag="PLN77_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRM44706.1"
                     /translation="MISKEKKQAIIKEYARTEGDTGSPEVQVAVLTARIEELTEHLKV
                     HHKDHHSRRGLLKMVGQRRGLLDYLKKTDIERYRALIEKLGIRK"
BASE COUNT          473 a          531 c          509 g          652 t
ORIGIN      
        1 ggctgccatc agagacatgg tggatgcaca gatacttgcc tgggaagtag aaccgttaga
       61 ctcgaaggtc tcggatacgg tacggattgc gtacgggaac tcttcctcgg tcggaagtac
      121 cggaaccagg gcacgttctg ccagagcacc atggccgatc tcacggcgtc ccggtccgcg
      181 ggacggtttt gtctcaccta cagagtagga cgggaagttg tactgatgca tgtaacgttt
      241 ggaggtctcg aactcatcca gtccgtccag tttctgtgct tcggaaagcg gagccagtgt
      301 tgttacgttg cagatctgag tctgtccacg ggtgaacata gcggaaccat gaacacgcgg
      361 gataatgtca acctctgctg ccagaggacg gatttctgtg atcgcacggc cgtccggacg
      421 tttgtggtct tttaagatca tcttacgaac ggtctttttc tgatactggt aaacagcctc
      481 gcctaatact gccagccact cttcgttgtc ggcaaatgcc tcctcgagtt tctcggtgat
      541 gacgcggatg ttttcttctc ttgtctgttt gtcatcgctg aaaacagcct cttccatctc
      601 tgccggagga acgatctctt tgatcgctgc aaacagctct tccggaatag cgcagctctc
      661 ataagcgtgt ttcggttttc caacctctgc tacgatgctg tcgatgaatt tgatgatttc
      721 ctggtttact tcgtgtgctt tgtagatggc gtcgatcatt tttgcttccg gaatctcttt
      781 tgctccggct tcgatcatga tgactttctc tctggtggaa gctacggtca gtttcaggtc
      841 ggatacggct ttctgtgcga gagtcgggtt tacgatgaac tcgccgtcga tcataccgat
      901 ctgagtcatg gcacacggac cgtcaaacgg gatgtcggaa atgcaggttg cgattgcgga
      961 acccagcatt gcgactactt ccggatcaca ctccggatct acggacatta ccatgttgtt
     1021 taatgtaaca tcgttgcggt aatcttttgg gaacagcgga cgcatcggac ggtcaatgac
     1081 acggctggtc agaattgcgt gctcgcttgc ttttccttct cttttgttga atccgcccgg
     1141 gatttttcca acggcgtaca tcttctcttc gtactcaacg cttaatggga agaagtcgat
     1201 gccgtcacgc ggtttctcag atgcggttgc tgtagaaaga actacagtat cgccataatg
     1261 cataaatgct gcgccgtttg cctgttttgc tacacggcct acgtctacgg tcagggttct
     1321 gcctgccagt tccatagaaa aactcttgta cataaataat ctcctttttt tcttctgagc
     1381 agaatttccg aaacaactga aaagcacacc ggaatacgga caggatgcag cttttcaatg
     1441 tgtttttcag tggtctcgga taaaattcct gcacctccag aacctgccac tggcagcttc
     1501 ttacggatgc aatgcagctc agaatggctc catgtcgttt gcgtctttga tcttacaagc
     1561 aaagaaacgg gcggaaaatg tatcccgccc gttgttccaa gctgattatt ttctgattcc
     1621 taacttctcg attaaagcac ggtatctttc aatatcagtt ttctttaagt agtcaagcag
     1681 accacgtctc tgaccaacca ttttcagaag acctcttctg gagtgatgat ctttgtggtg
     1741 aactttcaga tgctcagtga gctcctcgat tcttgcggta agaacagcta cctgtacttc
     1801 cggtgatcct gtatctccct cagttcttgc atactctttg atgattgcct gttttttttc
     1861 tttcgaaatc attttaaatt cctcctgtta ttttgattgc accgctgact aagaagtggt
     1921 cggagtgtcc tgcctctgaa tcagggtccg gggatctttt ttgtcccgca tactcgatta
     1981 gtatagcata cgcttttcga attgtaaata ctaaaatcag atgttttcgc gatgaatttt
     2041 cacgtagtta cagttctccg aactgtaact ttcgcagctc tcattttaag ttttgcttcg
     2101 caaaaggaga gctgctcaca cctgaatcac tttcttacgc agcgaaacag caagtgtttc
     2161 actgc
//
LOCUS       DAOMUF010000266         2163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14430_length_2163_cov_2.945920, whole genome shotgun sequence.
ACCESSION   DAOMUF010000266 DAOMUF010000000
VERSION     DAOMUF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14430_length_2163_cov_2.945920"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="PLN77_10365"
     CDS             complement(<1..1779)
                     /locus_tag="PLN77_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRM44707.1"
                     /translation="MEQELLQEIASYWGTRAEGYSEVNEKELAGSQREAWLHVLEEQF
                     PEKKKEEMKILDIGTGPGFFPMILSEAGYTVTAVDYTEEMLEKAKENLGKYTKYGLER
                     VTLQRMDAQNLEFADETFDVVISRNLTWNLEKPEQAYQEWMRVLKPGGVLLNFDANWY
                     GYLYDEEKKEAYEADRKKVEEQQLDDHYLCTDIDRMENIARQVPLSAMERPAWDTKVL
                     ESLGVCSIQTDSEIWKRVWSEEERLNYASTPMFLVRAEKSAEQSFQLGDVTVRRGEKY
                     QGDISFANGDIVLPGTIICGKLPGKTMLITGGVHSGEYVGIQACVELGAELQPEKTVG
                     TIVILKVLNRPAFENRAGSLGLSDGKNLNRVFPGNPNGTEMERLAWAITKEVYPKVDY
                     YIDLHSGDDFEALTPYVYYAGKAAQEVMETSRKMAEQVDVPYMVRSMVSSGGAYNYAA
                     SKGIASILLERGGMGAWTSEEVNSDKRDVRNILSSLGIYQIRRDVRNYVPMEVTDVRY
                     QAASESGLWYPAAKPGDMVAEGALLGIIRDYNGKLRETCRAEYTGVVLYQTGSLQVIE
                     GGSVVAYGRIVREPEYDDRKEQIVHYW"
     gene            1924..>2163
                     /locus_tag="PLN77_10370"
     CDS             1924..>2163
                     /locus_tag="PLN77_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HRM44708.1"
                     /translation="MIGGADLIICGRQTTDGDTAQVGPLIAKHLTIPHAAWVAEVTDV
                     TDHSITVRQDLVSVSQVSELPYPCLITIEFLERELI"
BASE COUNT          439 a          684 c          478 g          562 t
ORIGIN      
        1 ccagtaatgg acaatctgtt ctttgcggtc gtcatattcc ggctcgcgca cgatccgtcc
       61 gtaggcaaca acagatcctc cctcgattac ctgcaggctg cccgtctggt acagcacaac
      121 accggtgtat tcggcgcggc atgtctcccg gagttttcca ttataatcac ggatgattcc
      181 aagcagtgcg ccctccgcaa ccatgtctcc cggtttcgcc gccggatacc acaggccgct
      241 ttccgaagcc gcctgatagc ggacatccgt cacttccatc ggcacgtagt tccgcacatc
      301 gcggcggatc tgatatatgc cgaggctgct taagatattg cgcacatcac gcttgtcaga
      361 attgacctcc tcggaagtcc aggctcccat gccgccgcgc tctaagagga tgctcgcgat
      421 gcccttggac gccgcgtagt tgtacgctcc gcctgatgat accatggagc gcaccatgta
      481 cggtacatcc acctgctccg ccattttccg cgaagtctcc atcacttcct gcgccgcttt
      541 tccggcgtaa tagacatacg gcgtcaatgc ttcgaaatca tcgccgctgt ggagatcaat
      601 ataataatcc accttcggat aaacttcttt cgtaatggcc cacgccagcc gctccatctc
      661 ggttccgttt ggattgcccg gaaatacacg gttcaggttt ttgccatcgg aaagtccgag
      721 actgcccgca cgattttcaa aagcaggacg gtttaagacc tttaaaatca cgatcgttcc
      781 aaccgttttt tccggctgaa gctccgctcc cagttccaca cacgcctgaa tgccgacgta
      841 ttccccggaa tgaacgccgc cggtgatcag cattgttttt cccggaagtt ttccacagat
      901 aatcgttccc ggaagcacga tatccccatt cgcaaaggag atatccccct gatatttttc
      961 gccgcggcgg acggtcacat caccaagctg gaaggactgc tctgcacttt tttccgcgcg
     1021 caccagaaac atcggggtcg atgcatagtt cagtctctct tcctcgctcc agacgcgctt
     1081 ccagatttcg gaatcggtct gaatggaaca gacgccgagg gattcgagca ccttcgtatc
     1141 ccacgccggc cgttccatcg cagagagcgg cacctggcgc gcaatatttt ccatgcggtc
     1201 aatgtccgtg cacagataat ggtcgtcgag ctgctgctct tccactttct tccggtctgc
     1261 ctcgtacgct tcttttttct cctcgtcata gagataaccg taccagttcg catcaaaatt
     1321 gagcaacacg ccgcccggtt tcaggacacg catccactct tggtatgcct gctccggctt
     1381 ttccagattc caggtcagat ttctggaaat gacaacatca aaggtctcat ccgcaaactc
     1441 cagattctgt gcatccatcc gctgcagcgt cacgcgctcc agaccgtatt tcgtatattt
     1501 tcccagattc tctttcgcct tttccagcat ctcctccgtg tagtcaacag ccgtcaccgt
     1561 ataacctgcc tccgacaaaa tcatcgggaa gaatcccggg ccggttccga tatcgagaat
     1621 cttcatctct tctttctttt tctctggaaa ctgctcctct aatacatgca gccacgcttc
     1681 tctctggctt cccgccagtt ctttttcatt gacctcagaa tacccctccg cacgggttcc
     1741 ccagtaggag gcaatttcct gtaataattc ctgttccata tcttccgctt ctttcttctt
     1801 tttcttaact ttcccggcgc ggccgttttc caaccgctca aatatccctt tactctacca
     1861 aaaatcggcc tttttcacaa gcccccacgg gggatattct taacgccggc gcagggtatt
     1921 caggtgatcg gcggcgcgga tctgattatc tgcggacgtc agacaaccga cggcgatacc
     1981 gcgcaggtcg gccctttgat tgcgaaacat ctgacgatcc cgcatgccgc ctgggtcgca
     2041 gaggttaccg atgtcacgga tcattccatc accgtccgtc aggatcttgt gagtgtttcc
     2101 caggtttccg aactccctta tccgtgcctg attaccatcg agtttctcga gcgcgagctg
     2161 atc
//
LOCUS       DAOMUF010000267         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14470_length_2157_cov_4.600381, whole genome shotgun sequence.
ACCESSION   DAOMUF010000267 DAOMUF010000000
VERSION     DAOMUF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14470_length_2157_cov_4.600381"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PLN77_10375"
     CDS             complement(<1..990)
                     /locus_tag="PLN77_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM44709.1"
                     /translation="MSKETREYAKQLVSQMTLEEKMSQMVYQSPAIERLGIPAYNWWN
                     EALHGVARAGVATVFPQAIGLAATFDKELLQEIGDVVSTEGRAKFNEFSRKGDRGIYK
                     GLTFWAPNVNIFRDPRWGRGHETYGEDPYLTGELGCAYIRGLQGPDPDHPKAAACAKH
                     FAVHSGPEAIRHEFDARVSKHDLYDTYLYAFKRCVKDAKVEAVMGAYNRVNGEPACGS
                     KTLLKDILRDEFGFEGHVVSDCWAIIDFHEHHRVTKNVEESAARAVNNGCDLNCGIAF
                     LHLPKAYEDGLVSEEAITAAVERLMEIRIRLGMMKDYPSPYEDLSYDLVECKEH"
     gene            complement(1223..1828)
                     /locus_tag="PLN77_10380"
     CDS             complement(1223..1828)
                     /locus_tag="PLN77_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44710.1"
                     /translation="MLFKTYQKITTLFKEHHGYMSFADLRDHQVTVLQLAEMEEEGSL
                     ERFARGWYWCKDCGYEKPEDYQYIEIAKVNPRAVICMDSAAWLAGMLKEEPATIAVAT
                     ARTDRKKMDLENFEIRRFYFQNADVPGEICEKKTEFGSYKYYSPKRTFCDCLRMNNKM
                     PEEVKEEILTWCRKQKYSKEEILEYANKLRAVKNIEEEYQK"
BASE COUNT          462 a          570 c          444 g          681 t
ORIGIN      
        1 atgctccttg cactcgacta gatcgtagga cagatcttcg tacggtgacg gatagtcttt
       61 catcataccg aggcggatgc ggatttccat caggcgctca actgccgcgg tgattgcctc
      121 ttcggatacc aggccatcct cgtatgcttt cggcagatgc aggaaagcaa tgccgcagtt
      181 caggtcacag ccgttgttga cagcacgcgc tgcggactcc tctacatttt tggtcactct
      241 gtggtgctca tggaaatcta tgattgccca gcagtcggag acaacatggc cttcaaagcc
      301 gaactcatcg cgcaggatgt cctttaacaa tgtcttgctt ccgcatgccg gttcaccgtt
      361 gacacggtta tacgcgccca tgactgcctc gacttttgca tccttgacac agcgtttgaa
      421 ggcatacaga taggtgtcat acagatcatg tttgctgact cttgcgtcaa actcatggcg
      481 gatcgcctcc gggccgctgt ggacggcgaa atgtttggca cacgccgctg ccttcgggtg
      541 atccgggtcc ggtccctgta atccgcggat gtaggcgcat ccgagctcac cggtcagata
      601 cgggtcctca ccatatgtct catggccgcg gccccatctc ggatcacgga aaatgttgac
      661 gttcggtgcc cagaaggtca ggcctttgta gatgccgcgg tcgccttttc tcgaaaactc
      721 gttgaatttt gcacgtccct cggtagaaac aacatcaccg atctcctgca gcagctcttt
      781 atcaaacgtt gctgcaaggc caatagcctg cggaaatacc gttgccacac cagctcttgc
      841 cacgccgtgc agcgcttcat tccaccagtt gtatgccgga atgccaagac gctcgatcgc
      901 cggtgactga tagaccatct ggctcatttt ttcttccagc gtcatctggg aaaccagctg
      961 ttttgcatac tctctcgtct ctttactcat aggtaaaatc ctcccgaaat ttgttgcctc
     1021 tattgtatcg ccgcgtttcc cgttcggcta gtcaagtatt gctctttgct tctcatttta
     1081 tgcgattttt gcgaatgaca caggggaaca tacgaggaaa tcgtgtatgc gtaaccatcc
     1141 gatatacacg cagaaactcc cggcagaatt tctcgtataa gatcttccgt cgggagtttc
     1201 ccttatctat tcctgtgaaa ttttatttct gatactcttc ctcgatattt tttaccgcac
     1261 ggagcttgtt cgcgtattcc agaatctctt ctttggaata tttctgtttc cggcaccagg
     1321 tgaggatttc ttcttttact tcctccggca tcttgttgtt catgcgcaga cagtcacaaa
     1381 aggttctctt cggagaataa tatttgtagg aaccaaactc tgtctttttc tcacagattt
     1441 cgcccgggac atcggcattc tggaaataaa aacgtctgat ttcaaaattt tccagatcca
     1501 ttttttttcg gtctgtccgt gctgttgcaa ctgcaatcgt agccggctct tctttcagca
     1561 ttccggcaag ccatgcagca ctgtccatgc agatgactgc gcgtgggttt actttcgcaa
     1621 tttcaatata ttgatagtct tccggttttt cataaccaca gtccttgcac cagtaccagc
     1681 cgcgtgcaaa tcgttccaga cttccttcct cttccatttc tgccagctgc agtacggtca
     1741 cctgatgatc tctgaggtct gcgaagctca tatatccatg atgttcttta aataacgtcg
     1801 tgatcttctg gtatgtttta aaaagcatat tttctttccc ctttcaggat gtgatacatc
     1861 ttttttcatc tataggcgtt tgcacatata atattccaat tttcatggaa tggcaagcac
     1921 taatttgcaa aaaccatgtt ttttctctgt ttttttctta ttagctgctg ctaacatatt
     1981 tctcttttta tcacttataa gtataccttt tttacctgaa atatgcaacc tgctgatccg
     2041 ctttttctgt tctcatacca gcagaaaaag accggatttt ttacttctca tccggtcttt
     2101 ttctctttca ttctatcttt taacgtttac cgtaaaacct ttttcactct gccgctt
//
LOCUS       DAOMUF010000268         2152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14517_length_2152_cov_5.000477, whole genome shotgun sequence.
ACCESSION   DAOMUF010000268 DAOMUF010000000
VERSION     DAOMUF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14517_length_2152_cov_5.000477"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLN77_10385"
     CDS             <1..345
                     /locus_tag="PLN77_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRM44711.1"
                     /translation="GNEGFLAIENEVNATLEADKSVIAPGGSAIYGKEAMEHFKEIGT
                     IVYLKLSYEDLAKRLGNLKGRGVVLKEGQTLKDLYDERSKLYEQYAEITVDESGRDIQ
                     GTILALREQIGM"
     gene            683..1972
                     /locus_tag="PLN77_10390"
     CDS             683..1972
                     /locus_tag="PLN77_10390"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM44712.1"
                     /translation="MEQYVIRGGNPLVGEVEIGGAKNAALAILTAATMTDETVLIENL
                     PDVSDINVLLEAIAEIGATVDRIDRHTVRINASTIGDLSVDYEYIKKIRASYYLLGAL
                     LGKYKRAEVPLPGGCNIGSRPIDQHLKGFRALGAEVSIRHGAIVASAADLHGSHIFLD
                     MVSVGATINIMMAASMARGNTTIENAAKEPHVVDVANFLNSMGANIKGAGTDVIRIKG
                     VDTLHGTSYAVVPDMIEAGTYMFAAAATKGDVLIKNVIPKHLEATTAKLEEIGCDVEE
                     FDDSIRVSASKRLGRTHVKTQPYPGYPTDMQPQIAVTLALANGTSIVTESIFENRFKY
                     ADELARMGACIKVEGNTAIIDGVEKFTGAEVSAPDLRAGAALVIAGLAAEGVTVVDDI
                     VYILRGYEDFDGKLRGLGAEIERVNNAKDIQKFRLRA"
BASE COUNT          657 a          418 c          589 g          488 t
ORIGIN      
        1 ggaaatgaag gctttcttgc gattgagaat gaagtcaatg cgacactgga agccgacaaa
       61 agcgtcatcg cgccgggcgg cagtgccatt tacggaaaag aggcgatgga gcattttaaa
      121 gaaattggaa ccatcgttta cctgaagctg tcgtatgagg atctggcgaa acgtctcgga
      181 aacctgaagg gacgcggcgt cgtgctgaaa gagggccaga cgctgaagga tctctacgat
      241 gagagaagta aattatatga gcagtatgcc gagattacgg ttgacgagag cggaagggat
      301 atccagggca cgatcctggc gctccgcgag cagatcggca tgtaaaacag aattggtgga
      361 atattcaggg aaattttgaa aacatacgat tatatcataa taagggaaat gttctgatag
      421 ttagaaatta catataacgt ttctctgaat attcctgacc ggaaacgatg gaaaaagacg
      481 gaaatagcac agaatgtatt aaaattgatt tacaaagtat tagatttgag ttaagataag
      541 gggagactga aaaataactg cggggagcag tgcgtttcag cgtatctgga gtgtgtaaaa
      601 aaacaggaaa aagactttga ttcgttagat attggataaa acacactcgt taagtatgag
      661 aaagaaaatt tgaggtgaat atatggaaca gtatgtcatc agaggtggaa atcctcttgt
      721 gggcgaagtg gaaatcgggg gagcaaaaaa cgcggcgctt gcaattctta cggcagcaac
      781 gatgaccgat gagacggtcc tgatcgaaaa cctgccggat gtcagtgata ttaatgtact
      841 tctggaagca atcgcagaga tcggagccac cgtggaccgc atcgaccgcc ataccgtgcg
      901 gatcaatgca tcgaccatcg gggatctgag cgttgattat gaatatatca aaaaaatccg
      961 tgcgtcttat tatcttttag gcgcgctgct tggaaaatac aaaagagcgg aagttccgct
     1021 tccgggcgga tgtaacattg gaagccgtcc gatcgaccag catttaaagg gattccgcgc
     1081 actcggtgct gaggtaagca tccgtcatgg cgcaatcgtg gcaagtgcgg cagatctgca
     1141 tggaagccac attttcctgg atatggtttc cgttggagcc accatcaaca tcatgatggc
     1201 agcttcgatg gcaagaggaa atacaacgat tgagaatgca gcgaaagagc cgcacgttgt
     1261 cgatgtggcg aacttcctga acagcatggg agcaaacatc aaaggagcag gaaccgatgt
     1321 gatccggatc aagggtgtcg ataccctgca cggaaccagc tatgcggtcg taccggatat
     1381 gatcgaggca ggtacgtata tgtttgcagc agcagctaca aagggcgatg tgctgattaa
     1441 aaacgtgatt ccaaagcatc tggaagcaac aaccgcaaag ctcgaggaga ttggctgcga
     1501 cgtagaagag tttgacgatt ccatccgtgt gagcgcaagc aaacgtctgg gcagaacaca
     1561 cgtaaagaca cagccgtatc cgggttaccc gaccgatatg cagccgcaga ttgcggttac
     1621 actggcactg gcaaacggta cgagtattgt aacagagagt atttttgaaa atcgttttaa
     1681 atatgcggat gagctggcac gtatgggcgc ctgtattaaa gtagaaggca acacggcgat
     1741 catcgacggc gtagagaaat ttaccggagc agaggtttcc gcaccggatc ttcgcgccgg
     1801 agcagcactt gttattgcag gtcttgcagc agagggtgtt accgtggtgg atgatattgt
     1861 atacattctg cgcggatacg aggacttcga cggaaagctc cgtggactcg gcgctgaaat
     1921 tgagcgtgtg aataatgcaa aggacatcca gaaattccgg ttaagagcgt aaaaacagta
     1981 taaaacaaat gttgacaaaa aggacggtgc aataaaaggt accgttcttt tattttacct
     2041 tattgcgaag aaattggctt aaattttact actattattg tgaattatta ttacctgtga
     2101 acctcacatg actaaagtca cgtgcttcag accgctttag gcggcagaca aa
//
LOCUS       DAOMUF010000269         2147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14556_length_2147_cov_3.203155, whole genome shotgun sequence.
ACCESSION   DAOMUF010000269 DAOMUF010000000
VERSION     DAOMUF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14556_length_2147_cov_3.203155"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLN77_10395"
     CDS             complement(<1..193)
                     /locus_tag="PLN77_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44713.1"
                     /translation="MKKEELFEVLGNLEPGMVEKARSDRHPRRGVWKKWTAAAACAVI
                     IGGAVLGVATWRNGREGSAV"
     gene            complement(190..750)
                     /locus_tag="PLN77_10400"
     CDS             complement(190..750)
                     /locus_tag="PLN77_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM44714.1"
                     /translation="MEDTHIVQMYWDRNETAISESAMKYGTYCTSIAQNILRNPADAE
                     ECVNDTWLHAWNAMPPHRPSLLSTFLGKITRNLSFDLYRKMHRKKRGESQMDAVLDEL
                     EECVSGKDDIERQWEMKELIAEINQFLQKLPEEKRCMFVLRYWYVDSIGEIAERLGRS
                     ENYISVSLNRIRGKLHTHLTERGFEL"
     gene            complement(874..>2147)
                     /locus_tag="PLN77_10405"
     CDS             complement(874..>2147)
                     /locus_tag="PLN77_10405"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRM44715.1"
                     /translation="MVTASHNPAKYNGYKAYGPDGCQMTDDAAAIVYAEIQKTDILTG
                     VKYMSFAQGVEEGLIRFVGDDCKNALYEAIESRQVRPGLCKTAGLKLVYSPLNGSGLV
                     PVTRVLKDIGITDITIVPEQEYPNGYFTTCSYPNPEIFAALEKGLDLAKETGADLMLA
                     TDPDADRVGIAMKCPDGSYELVTGNEVGVLLLDYICAGRIEKGTMPEKPVAVKSIVST
                     PLADAVAEHYGVELRSVLTGFKWIGDQIANLEADGEVDRFIFGFEESYGYLAGPYVRD
                     KDAVIGSMLICEMAAYYRSIGSSLKQRLEEIYAEYGRYLNKVDSFEFPGLSGMDKMAG
                     IMDGLRKEPLTEIAGSAVVKVTDYEKPEETGLPSANVLIYKLANGETVIVRPSGTEPK
                     IKVYYTTLGKDLAEAEAEKEKLAEALKPILA"
BASE COUNT          446 a          601 c          482 g          618 t
ORIGIN      
        1 ggactgcaga accctcccga ccgtttctcc atgtcgcaac ccccagcaca gctccgccga
       61 tgatcaccgc gcaggctgcg gcggccgtcc attttttcca tacgcctctt cgcgggtggc
      121 gatcactccg tgctttttcc accataccag gctccagatt tcccagcact tcaaacaatt
      181 cttctttctt cataattcga accccctttc cgtcaagtgt gtatgcaatt ttccacggat
      241 ccggtttaat gaaactgaaa tataattttc acttcttccc agccgctccg cgatttcacc
      301 gatactgtct acataccagt accgcaggac aaacatgcat cgtttctcct ccggcagctt
      361 ctgcagaaac tgattgatct ccgcgatcag ttctttcatc tcccactgcc tctcgatatc
      421 atcttttccc gaaacacatt cttccagctc gtccaatacc gcatccatct gactttctcc
      481 gcgttttttg cgatgcattt ttctatacag atcaaacgaa agatttctcg ttatttttcc
      541 aagaaatgtg gataacagag acggccggtg cggcggcata gcgttccacg cgtgaagcca
      601 ggtgtcgttg acacattctt ctgcatccgc cggattccgc agaatattct gtgcaatgga
      661 cgtacagtat gtaccatatt tcatcgcaga ctcgctgatc gctgtttcgt tcctgtccca
      721 gtacatttgg acaatatgag tatcctccat atggaaatcc cccctttcat ctatcatcac
      781 gccattttgc agaaaatctt acacttttca aaagaaattt cggttcaaaa aagggcgtga
      841 ccaaagccac gcccttccat tcttttattt tctttatgcc agaatcggtt ttaaagcctc
      901 tgcgagtttt tctttctctg cttctgcctc tgccagatcc ttaccaagtg ttgtgtagta
      961 tactttgatc ttcggctctg ttccggacgg gcggacgatg acggtttcac cgtttgccag
     1021 tttgtagatc agaacgtttg cggatgggag tcctgtttct tccggtttct cgtaatcggt
     1081 tactttgaca acagcggaac ctgcgatctc ggtcagcggc tcttttcgaa ggccatccat
     1141 aataccggcc atcttgtcca ttccgctaag tcccgggaac tcgaaactgt ctactttgtt
     1201 caggtagcgg ccatactcgg catagatctc ttccagtctc tgtttcagag agcttccgat
     1261 gcttctgtag taagctgcca tctcgcagat cagcatggag ccgatgactg cgtctttatc
     1321 tcttacatac gggccagcca ggtatccgta gctctcctcg aatccgaaga tgaagcggtc
     1381 tacttcgccg tctgcctcaa ggtttgcaat ctgatcgccg atccatttga atccggtcag
     1441 aacgctgcgg agctccacgc cgtagtgctc ggcaacagca tctgccagtg gtgtggatac
     1501 gatggatttt acagcaactg gtttctccgg catggtgcct ttctcaatgc ggcctgcgca
     1561 gatgtagtcg agaagcagaa cgccgacttc gtttccggtt accagctcat agcttccatc
     1621 cgggcatttc atggcgatac caacgcggtc tgcatccggg tcggttgcca gcatcaggtc
     1681 tgcgccggtc tcttttgcaa gatccaggcc tttttccagt gcagcaaaga tttccgggtt
     1741 cggatagctg caggttgtga agtatccgtt cggatactcc tgctccggaa cgatggtgat
     1801 atcagtgatg ccgatgtctt ttaatacacg ggtaaccggt acaagaccag atccgttcag
     1861 cgggctgtaa acgagtttca gtccagcggt cttgcagagg cctggacgaa cctgacggga
     1921 ttcgatggct tcgtacagtg cattcttgca gtcatcgccg acgaaacgga tcagtccctc
     1981 ttctactccc tgtgcaaacg acatatattt tacgccggtc aggatatcgg ttttctgaat
     2041 ctctgcgtat acgattgctg cagcatcatc ggtcatctgg catccgtccg ggccgtaggc
     2101 tttgtagccg ttgtattttg ccgggttgtg ggatgcagtt accatga
//
LOCUS       DAOMUF010000270         2147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14557_length_2147_cov_3.072658, whole genome shotgun sequence.
ACCESSION   DAOMUF010000270 DAOMUF010000000
VERSION     DAOMUF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14557_length_2147_cov_3.072658"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLN77_10410"
     CDS             complement(<1..897)
                     /locus_tag="PLN77_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRM44716.1"
                     /translation="MREGYTSQAKKALAYAVRTAEKCGHNYVGTEHLLIGLLNVEDGT
                     AGMVLSEFHVDAGQLTELIDRLIAPGGNTVTESRPGYTPRVRRVLENAEAEAARFKSR
                     AVGTEHLLIAILKESDCVATRLLFTMGINIQKLYGAILTAMGEAPLQGGASGNANTGR
                     GPQTRGGAQTSETPALDQYSRDLTELAREGKLDPVVGRGQEIERVIQILSRRTKNNPC
                     LIGEPGVGKTAIAEGLAQRIALGIVPETIRDKRVVVLDLSGMVAGSKYRGEFEERIKR
                     VVKEVTDNENILLFIDELHTIIG"
     gene            complement(914..1300)
                     /locus_tag="PLN77_10415"
     CDS             complement(914..1300)
                     /locus_tag="PLN77_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM44717.1"
                     /translation="MYYETIQIDFNSDEAIYIQLRNQIILGIATSRIQEGDTLPSVRQ
                     LADNIGINMHTVNKAYSVLRQEGLLSIDRRKGAVISIDADKLYAMQELREDLQVILAK
                     AVCKNISKEEVHQLVDEIYSNYADET"
     gene            complement(1380..2144)
                     /locus_tag="PLN77_10420"
     CDS             complement(1380..2144)
                     /locus_tag="PLN77_10420"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRM44718.1"
                     /translation="MILAIDIGNTNIVLGCVEGKKTYFIERLSTDKAKTELEYAVSLK
                     NVLELYEIPVEQIEGGIISSVVPQVTELCRSAAEKVIKKPVKVVGPGVKTGLNIKLDD
                     PATAGSDLVVVAVAALNEYTCPQILIDMGTATTMSVLDQKGSFIGGVILPGLRTSLDA
                     LVSNAAQLSQTSLTAPKKVIGKNTLDCMKGGMIYGNAASIDGMIERMEEELGCKCTIV
                     ATGGLAKAVIPHCRHDIIIDENLLLKGLQIIYEKNR"
BASE COUNT          455 a          582 c          471 g          639 t
ORIGIN      
        1 cccgatgatg gtgtgaagtt catcgatgaa cagcagaata ttttcattgt ccgtcacttc
       61 tttgacgaca cgtttgatac gctcttcgaa ctcaccgcgg tatttgcttc cggctaccat
      121 tccggaaagg tcaaggacaa caactcgctt atcgcggatc gtttccggca caatgccaag
      181 ggcaatccgc tgcgccagac cttccgcaat cgccgttttt ccgacacccg gctctccgat
      241 cagacacggg ttgttttttg tccggcgact taagatctga atgacacgct caatttcctg
      301 cccacggccg acaaccggat cgagttttcc ttcgcgcgca agctcagtca gatcccggct
      361 gtactggtcc agtgccggcg tttcgctggt ctgcgctcca cctcttgtct gcggtccgcg
      421 gccggtattt gcatttccgc ttgcaccgcc ctgcagcggt gcctcgccca tggcagtaag
      481 gattgctcca tatagtttct gaatgttgat ccccatggta aaaagcaaac gggtagcaac
      541 acagtcactt tcttttaaga ttgcgatcag cagatgctcg gttcccacag ctctgctctt
      601 aaaacgcgct gcctccgctt ctgcattttc aagcactctg cgtacccgcg gagtatagcc
      661 cggtcgggat tcggtcacgg tgtttccgcc tggtgcaatc agacggtcaa tcagttcggt
      721 aagctgtccg gcatctacgt gaaactcaga aagcaccatg cctgctgtgc catcctccac
      781 gttcagaagt ccgatcagaa ggtgttcggt tcctacgtaa ttatgtccac atttttcagc
      841 tgtcctaacc gcatatgcca gtgctttttt tgcctgactg gtatatcctt ctctcataat
      901 actattgtct ccttcatgtt tcgtctgcgt aattgctgta aatttcatct acaagctgat
      961 gtacttcttc ttttgatata tttttgcaga cggcttttgc aagaatcacc tgcagatctt
     1021 cgcgcagttc ctgcatggca tacagtttat ccgcatcgat agagataacg gctccttttc
     1081 ttcggtcaat cgaaagaagc ccctcctgcc ggagtacgct gtaggcctta tttacggtat
     1141 gcatgttgat tccgatattg tctgcgagct gtcttaccga aggtagagta tctccttcct
     1201 gaattctgga agtggcaata cccagaataa tctgatttct cagctggata tagatcgctt
     1261 catcgctgtt gaaatcgatc tgtattgtct cataatacat agttacaccc tgctttctcg
     1321 tatcgttttg ttatacatat gttataacaa ttcgcaaact ttgtcaacaa acggtttccc
     1381 tacctgtttt tctcgtagat gatctgaaga cctttcagga gcagattctc atcgatgatg
     1441 atgtcatgtc tgcagtgcgg aatcacagct ttcgccagac ctccggtagc cacaatcgtg
     1501 catttgcagc caagttcttc ttccatccgt tcgatcatgc cgtcgatgct ggcagcgttt
     1561 ccataaatca taccgccttt catacagtcc agcgtatttt tgccaatgac ttttttcggt
     1621 gctgtcagac tggtctggct aagctgcgcg gcatttgata cgagagcatc cagcgaagta
     1681 cgaagtcccg gaagaatcac gcctccgatg aagcttcctt tctgatcaag tacactcatc
     1741 gtcgtggcag ttcccatatc gataagaatc tgcggacatg tgtattcatt cagcgctgct
     1801 actgctacaa caacaagatc gcttcctgcc gttgcggggt cgtccagttt gatattaagt
     1861 ccggtcttca caccggggcc gacaactttg accggctttt tgatgacttt ttctgccgca
     1921 ctccggcaga gctctgtcac ctgcggaaca acggaagaga tgatacctcc ttcgatctgc
     1981 tccaccggaa tctcatacag ttccagtaca tttttcaggc tgactgcgta ttccagctct
     2041 gtttttgcct tgtccgtgga gagtctttcg atgaagtatg tttttttccc ttccacacac
     2101 ccaaggacga tgttcgtgtt gccgatgtct attgcaagga tcatgat
//
LOCUS       DAOMUF010000271         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14702_length_2131_cov_3.164258, whole genome shotgun sequence.
ACCESSION   DAOMUF010000271 DAOMUF010000000
VERSION     DAOMUF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14702_length_2131_cov_3.164258"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLN77_10425"
     CDS             complement(<1..932)
                     /locus_tag="PLN77_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM44719.1"
                     /translation="MEKKWWHKTVGYQIYPKSFQDTNGDGIGDLKGVIRHLDKIEKLG
                     ANVIWLCPVFASPMVDNGYDISDYMDIDPSFGTMEDMKELIAEAKKRGIRILMDLVVN
                     HSSDRHAWFQAALKDPFGKYGKYYVFREGKDGKEPNNWRSIFGGSAWEKVPGYDNLYY
                     LHIFTKEQPDLNWENPELREEIYEMILKWMDLGLGGFRLDAISHLKKNYQYTNLPPDG
                     PDEYNMAFEYFNNVDGLADILCEMKERTFAPTDALTIGEYDHMGPEDVEDVIGENGSF
                     SSVFDFCHTLDNVRNPKWGNTVALFDDYRDQLFAA"
     gene            complement(1082..>2131)
                     /locus_tag="PLN77_10430"
     CDS             complement(1082..>2131)
                     /locus_tag="PLN77_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HRM44720.1"
                     /translation="WGQRVPSYREYITSPSPDAALWQTGWWKQLREIPSKTKVPIYLL
                     SGWYDHHHGSSMKTWERLNEETKQHSWLEIGAWNHFFQICLEDKEVLHPQNEEIPKML
                     EWFELTLKQKEIPTQRIRAYEIGADRWIDIVSDRLGEEGTAGSMTLYLDEKTLREDAP
                     ERGKTRNYTFDPATPVESIGGEALLHTMPQIGSHLQPEPDYREDVLSFVSEPLEETFT
                     LNGKASVRLFVESDCEDTAFTAKIMEVTPEGKAYNIRSSIATLEQGKTEAGAYEPGSV
                     AEVQITMWNIVYTIPKGSRIRVDVSSSDFPQYHVHSNQKGLWSEKTENKIAHQTIHMG
                     GEAASCVILPLMKKQ"
BASE COUNT          457 a          596 c          457 g          621 t
ORIGIN      
        1 gccgcaaaaa gctgatcgcg ataatcgtca aacagcgcca cggtattgcc ccatttcggg
       61 ttacgcacat tgtcgagggt gtgacaaaaa tcgaaaacgg acgagaaact gccgttttcc
      121 ccaatcacat cctccacatc ctccggtccc atgtgatcgt actctccgat cgtcagcgca
      181 tcggtcggcg caaaggtgcg ctccttcatt tcacacaaga tatcggcaag accgtctaca
      241 ttgttgaaat actcgaacgc catattgtac tcgtccggtc catccggcgg aaggttggtg
      301 tactgataat tctttttcag atggctgatc gcatcaagac ggaatccgcc aagtcccaga
      361 tccatccatt ttaaaatcat ctcataaatt tcttcccgca attccggatt ctcccaattg
      421 agatccggct gctcttttgt gaagatgtgg aggtaataca ggttatcata tcccggtact
      481 ttctcccatg cgctgccgcc aaaaatcgac cgccagttgt tcggctcttt gccgtctttt
      541 ccttcccgga acacataata ctttccgtac tttccaaacg gatctttgag tgccgcctga
      601 aaccacgcat gccggtcaga ggaatgattc acgacaaggt ccatcagaat gcggatgccg
      661 cgtttttttg cctccgcaat cagctccttc atgtcctcca tcgttccaaa cgacgggtcg
      721 atatccatgt aatcggaaat atcgtatccg ttatccacca tcggggatgc aaacaccggg
      781 cagagccaga tgacattggc tcccagcttt tctattttgt caagatgacg gatcacgcct
      841 ttcagatctc cgatgccgtc cccgttggta tcctgaaaac tcttcgggta aatctgatat
      901 ccgaccgttt tgtgccacca ctttttctcc attactcttc tcctgttcat tttgttttta
      961 tgatgcgggc cgttctttag gtttaccgca aaacctatag tgttttcact atatcacaaa
     1021 agaatggatt gtgctacttt tttctttttg atttttccta tgttcgcctt accgttacgg
     1081 ttcactgttt tttcatcagc ggcaaaatca cgcaggaagc agcttctccc cccatatgaa
     1141 tggtctgatg tgcaatttta ttctctgttt tttccgacca cagccctttc tgattgctgt
     1201 gaacatgata ctgcgggaag tcggaggagg aaacatctac acgaatgcgg gaaccctttg
     1261 gaatcgtgta tacaatattc cacatagtaa tctgtacctc tgccacgctg cccggttcat
     1321 aggctccggc ctccgttttt ccctgctcca atgttgcaat cgaggaacga atgttgtacg
     1381 cttttccttc cggcgttacc tccatgattt tagcggtaaa tgccgtatcc tcgcagtcac
     1441 tttccacgaa cagacggacg gaagcttttc cgttcagcgt gaaagtttct tccagcggtt
     1501 ctgatacaaa actcaggaca tcctcacgat agtccggttc cggctgaaga tggctgccga
     1561 tctgcggcat cgtatgcaga agggcttctc cgccgatact ctcgacggga gtggccgggt
     1621 caaacgtgta gtttctcgtt tttccccgtt ccggcgcgtc ttcccgcagt gttttctcat
     1681 ccagatacag cgtcatgctt cccgcagttc cttcttctcc cagtcggtcg gatacgatgt
     1741 caatccaccg gtccgctccg atttcatagg cacggatccg ctgtgtcggg atttcttttt
     1801 gtttcagcgt cagctcaaac cattccagca tctttggaat ttcttcattc tgcggatgca
     1861 gcacctcttt atcttcaaga caaatctgga aaaagtggtt ccatgcaccg atttccagcc
     1921 agctgtgctg tttcgtctcc tcattcagtc gctcccacgt tttcatactg ctgccgtggt
     1981 gatgatcgta ccagccgctt aaaagataaa tcggaacctt cgtttttgat ggaatttccc
     2041 gcaactgttt ccaccagccc gtctgccaga gtgccgcatc cgggctcggg cttgtgatgt
     2101 attcgcggta ggacggaaca cgctgtcccc a
//
LOCUS       DAOMUF010000272         2116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_14848_length_2116_cov_1.479864, whole genome shotgun sequence.
ACCESSION   DAOMUF010000272 DAOMUF010000000
VERSION     DAOMUF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14848_length_2116_cov_1.479864"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLN77_10435"
     CDS             complement(<1..348)
                     /locus_tag="PLN77_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HRM44721.1"
                     /translation="MRKTIDLQLFADAVRGKKIVYLYRLKKDAAKNAATALAFTTENG
                     RTTSKDADTTETKDGTIRTPGAAEVEITATSILAKGDTLIDSLEDAMINDELVEIWEA
                     NLDDPASSGSNKFK"
     gene            complement(358..744)
                     /locus_tag="PLN77_10440"
     CDS             complement(358..744)
                     /locus_tag="PLN77_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44722.1"
                     /translation="MDPQQELFSALLLELKKQYPGSVYDTFLPSEGTPYPFVYLADSD
                     LNDRANKTAVFGIVSQTIHVWHDNPRQRGTVSQMILQIKQICRQLEHTGSFSWSVQDL
                     NQRILPDTTTNQPLLHGIVEVTFLFS"
     gene            complement(749..1084)
                     /locus_tag="PLN77_10445"
     CDS             complement(749..1084)
                     /locus_tag="PLN77_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HRM44723.1"
                     /translation="MPKIKLEGMEKLQVKLKKNVQMSDVKRIVKSNGAALQESAQRKV
                     PVDTGNLKRSIGLEIRDSGLTAEVEPTAEYAAYVEYGTRYMNAQPYMRPSYTAQKEKF
                     KSDLKKLTR"
     gene            complement(1084..1386)
                     /locus_tag="PLN77_10450"
     CDS             complement(1084..1386)
                     /locus_tag="PLN77_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44724.1"
                     /translation="MRYDTPIFFQRVLPGEYDSKTGNYAADQVTEVQKMASVMDTRAE
                     IMQIVYGGIRQGSVTVQLQNHYQKPFDRIRIGNTNYKVDYTRKLRVKQTFILSEVV"
     gene            complement(1383..1709)
                     /locus_tag="PLN77_10455"
     CDS             complement(1383..1709)
                     /locus_tag="PLN77_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HRM44725.1"
                     /translation="MIEDLKLLLGMEDTDKKTEQQLQLILNATKQRLKFLLGGLEPPE
                     EMEYIILDVSVIRFNRIGSEGLSSHSVEGESLSWSENDFAGYMDDIQSYLDSQREARK
                     GKVKFL"
     gene            complement(1720..1944)
                     /locus_tag="PLN77_10460"
     CDS             complement(1720..1944)
                     /locus_tag="PLN77_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44726.1"
                     /translation="MYRVIEYFTDLQDDDHEYRVGDTFPREGLKVSEARLAELASAEN
                     LRGIPLIELVEPEKAGKGKSKNKAVDSLAE"
     gene            complement(1961..>2116)
                     /locus_tag="PLN77_10465"
     CDS             complement(1961..>2116)
                     /locus_tag="PLN77_10465"
                     /inference="COORDINATES: protein motif:HMM:NF037320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44727.1"
                     /translation="AVKYGDDVKNWSKWDGESEIASTAGHHITLVECDQNYKAVRSGD
                     VTVAVKS"
BASE COUNT          451 a          576 c          436 g          653 t
ORIGIN      
        1 tttaaattta ttgcttccgc tggatgctgg atcatccaga tttgcttccc agatctcgac
       61 cagttcatca ttgatcatgg catcttcaag agagtcgatc agtgtgtcgc ccttggcaag
      121 aatactggtt gccgtaatct caacctcggc tgctcccggg gttcgaatcg tgccgtcctt
      181 ggtctctgtg gtatctgcat ccttgcttgt cgttcttccg ttttctgtcg taaaggctaa
      241 tgctgtagct gcatttttag ccgcgtcttt tttaaggcgg tacagataaa cgatcttttt
      301 accacgtacc gcatctgcga ataactgtaa atcaattgtt tttctcatgc tgttctccta
      361 actgaataaa aaagtcactt ccacgatgcc gtgaaggagt ggctggttgg tagttgtgtc
      421 cggcaatatt ctctgattca agtcctgcac ggaccaggag aagctgccgg tatgttccag
      481 ttgtctgcaa atctgcttga tctgcagaat catctgtgaa actgtgcctc gctgccgcgg
      541 attgtcgtgc cagacgtgga ttgtctggct tacaatgccg aacacggctg ttttattggc
      601 tctgtcattc aaatcactgt ctgccagata gacaaacgga tatggtgtgc cttccgacgg
      661 taaaaacgtg tcatacacac tgcctggata ctgttttttc aattccagaa gcaatgcact
      721 gaataattcc tgctgtggat ccatcgtgtc acctcgtaag ctttttcaga tcagatttga
      781 atttctcttt ctgtgctgta taggaagggc gcatatacgg ctgtgcgttc atatatcggg
      841 ttccatactc cacgtatgcc gcatactctg ctgtaggctc cacttccgct gtaagaccgc
      901 tatcccggat ctcaagtcca atgcttcgtt tcaagttacc agtatccacc ggtacctttc
      961 tctgcgccga ctcctgcagg gctgccccat tacttttcac aatccgcttt acatcgctca
     1021 tctgcacgtt tttcttcaat ttgacctgca gtttttccat tccttccagc ttgattttcg
     1081 gcatcagacc acctccgata gtatgaacgt ctgttttaca cgcaatttcc gtgtatagtc
     1141 cactttatag tttgtgttcc cgatccggat ccgatcaaac ggcttctgat aatgattctg
     1201 aagctgcact gtcacgctgc cctgacggat tcctccgtat acgatctgca tgatctctgc
     1261 tctcgtatcc atcacagaag ccattttctg cacctctgtg acctggtctg cagcatagtt
     1321 tccggttttc gaatcatact cacccggcag gactcgctgg aagaaaattg gcgtatcgta
     1381 tctcacagaa acttcacctt tcccttcctt gcctcccgct ggctgtccag ataagactga
     1441 atatcatcca tgtacccggc aaaatcattt tcagaccagg aaaggctctc accctcaacg
     1501 ctgtgagagg agagcccttc cgagccgatc cggttgaatc gaatgaccga aacatccaat
     1561 atgatgtatt ccatctcttc cggcggctcc agaccgccaa gaagaaattt cagccgctgt
     1621 ttcgtggcat tcagaatcag ctgtaactgc tgttctgtct ttttgtctgt atcttccatt
     1681 ccaagaagca gtttcagatc ttcgatcata ggctgcctcc tattctgcca aggaatctac
     1741 tgccttattc ttgcttttcc ctttgcctgc cttttccggc tccaccagct cgatcagtgg
     1801 gataccacgc agattttcag cagatgcaag ctccgccagg cgggcttcgg ataccttaag
     1861 cccctcacgc gggaaggtat cgcctactct gtattcatgg tcatcgtcct gtaaatcagt
     1921 aaaatactcg attactctgt acatatacca tcatcctttc tcagcttttc acggctactg
     1981 ttacgtcacc ggaacgaact gctttgtagt tctggtcgca ctcaaccaat gttatatgat
     2041 ggccagcggt agatgcgatc tcggattctc catcccattt gctccagttt ttcacatcat
     2101 cgccgtattt taccgc
//
LOCUS       DAOMUF010000273         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_15903_length_2007_cov_3.788934, whole genome shotgun sequence.
ACCESSION   DAOMUF010000273 DAOMUF010000000
VERSION     DAOMUF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15903_length_2007_cov_3.788934"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1375)
                     /locus_tag="PLN77_10470"
     CDS             complement(158..1375)
                     /locus_tag="PLN77_10470"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRM44728.1"
                     /translation="MIQIAVLGYGTVGSGVVEVINTNHDSINKRAENEINIKYVLDLR
                     DFPGDPVEKVLVHDYEVIANDPEVDVVVEVMGGVEPAYTFTKRALEAGKSVCTSNKEL
                     VARHGTELLAIAKEHGANYLFEASCGGGIPIIRPLNSSLTADEIDEVTGILNGTTNYI
                     LSKMASDGSDFADVLKEAQRLGYAERNPEADVEGYDACRKIAILSSLAYGKEVNYEEV
                     YTEGITKITAEDIKYATSMGTAIKLLATSRKVGDQYYAMVSPVMIDAKNPLYSVNGVF
                     NAIFIHGNVLGDAMFYGSGAGKLPTASAVVADVVDCAKNKGRNIMMSWSEEKLDLLQI
                     DDVKSRFFVRVSGHAAERQSEIEELFGKVEIVAVPSLSEEFAFITGEITEQEYREKAE
                     KLDGIQSRIRARI"
     gene            complement(1406..1852)
                     /locus_tag="PLN77_10475"
     CDS             complement(1406..1852)
                     /locus_tag="PLN77_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRM44729.1"
                     /translation="MREEKTKYYVIKEKAVPEVLLKVIEAKRLMDMQELTVQQATEKA
                     GISRSSFYKYKDDILPFHDNAKGKTITFILQMDDEPGLLSDVLKIIAEFHGNILTIHQ
                     TIPLNGVASLTISVDIAEDRGDASEMINEIESRQGVHYFKILGQES"
BASE COUNT          425 a          535 c          428 g          619 t
ORIGIN      
        1 tcctgtatcc ggcatcggaa ccggtgggat atgatacgga ttcctttgga tccggcacag
       61 atctttaaaa ataaatctta agataactaa aaagacgttt cacacccctt gctttacggc
      121 tgtgaaacgt ctttttagtt atgtctattt cgagatctta aattctcgcg cggattctgc
      181 tctggattcc gtcgagtttc tctgcttttt ctctgtactc ctgctctgtg atttcgccgg
      241 taataaatgc gaactcctcg gacagacttg gaactgctac gatttctact tttccgaaca
      301 gctcctcgat ctcactctgg cgctctgcgg cgtggccgga cacgcggacg aagaagcggc
      361 tctttacatc atcgatctgc agcaggtcca gtttttcctc gctccagctc atcatgatgt
      421 ttctgccttt gttctttgcg cagtctacga catcggctac aacggcactt gcggtcggaa
      481 gctttcctgc tccgcttccg tagaacatcg catcacccag tacatttccg tggatgaaga
      541 tggcgttgaa gacaccgttt acgctgtaca gcgggttttt cgcgtcaatc atcaccgggc
      601 ttaccatggc gtaatactga tcacccacct tgcggcttgt tgcgagcagt ttgattgcgg
      661 ttcccatcga ggtcgcatat ttgatatcct ctgcggtgat ctttgtgatt ccctcggtgt
      721 aaacctcttc gtaattgact tcttttccat aagcaagaga agaaagaatc gcaattttac
      781 ggcacgcatc gtagccttct acatctgctt ccgggttgcg ctctgcgtag ccgaggcgct
      841 gtgcctcttt taatacatcc gcgaaatcgg aaccgtcgct tgccattttg cttaagatat
      901 agttcgtggt accatttaag atgccagtta cttcatcgat ctcatctgca gtcagggagg
      961 agtttaacgg acggatgatc gggataccgc cgccgcagct tgcctcaaac agatagtttg
     1021 cgccgtgctc ttttgcaatt gcaagaagct cggtaccgtg tcttgccacc agctctttgt
     1081 tggatgtgca gacgctcttt ccagcctcca gcgcacgttt tgtgaaggtg taagccggct
     1141 caacgccgcc catgacctcg acaacaacat caacctccgg atcgtttgcg atcacttcgt
     1201 aatcatgtac cagcactttt tccactggat ctcccgggaa atctcttaaa tccagaacgt
     1261 atttgatatt aatctcgttc tctgctctct tgttgatgct gtcatggttc gtgttgatga
     1321 cttctaccac gccggaacca acggtgccat atcctaatac tgcaatctga atcatctctt
     1381 ttgtccctct cttgcttttt ctttttcagg actcctgtcc taagatttta aaataatgaa
     1441 ctccctgtct ggactcaatc tcgttgatca tctccgaggc gtctccccgg tcttccgcga
     1501 tatccacgct gattgtcagt gacgccacgc cgtttaacgg aatcgtctga tgaatggtca
     1561 gaatgtttcc gtgaaactcg gcgataatct tcagcacatc ggaaagcagt cccggctcat
     1621 cgtccatctg cagaataaac gtaatcgttt ttccttttgc attgtcatga aaaggaagaa
     1681 tatcatcttt gtatttatag aaagagcttc tgctgattcc cgctttttcc gttgcctgct
     1741 gcacggtaag ctcctgcata tccatcaacc gtttggcctc gatgaccttc aaaaggacct
     1801 ccggaaccgc cttttctttg attacataat actttgtttt ctcttctctc atggcaatcc
     1861 tctgtttcta tgttctttcc aggaaataca tttgttttca caggaaagat cgtaccatat
     1921 tccggcacaa tgcgcaagga ctttttagaa tccgatactt ccgccgccgt ggctgtttcc
     1981 gctggcggat gtgtgaacgt ggcttcc
//
LOCUS       DAOMUF010000274         2002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_15958_length_2002_cov_2.772470, whole genome shotgun sequence.
ACCESSION   DAOMUF010000274 DAOMUF010000000
VERSION     DAOMUF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15958_length_2002_cov_2.772470"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="PLN77_10480"
     CDS             complement(<1..1821)
                     /locus_tag="PLN77_10480"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRM44730.1"
                     /translation="MAIKVSENGRLFTLQTKDSSYQMFADDKNVLLHLYYGEKIGEEN
                     LSGLIFCTDMGFAGNPEEAGPNRKYSLDTLPQEIPGSGVGDFRDDMIDIRHADGSFAA
                     DFRFDSFEILDHSYAIPGMPALYDTEKEKGETLVITMTEKASDIVLKLFYGVFENENV
                     ITRAARLENHGETAIELEKMLSFSMDLMYENYEMIYFSGRHAMERTAERIPVQHAKVE
                     IGSTRGTSSHHYNPAVILCEEGAGETHGSCIGACLVYSGNFVAAAQKDQKNQTRFQMG
                     IHPTNFCFHLEKGEAFDTPQAILSYSGTGLTKLSQQYHEIIREHICRGAYKHAKRPIL
                     INNWEATYFDFNEEKILKIAEQAQKLGIEMLVLDDGWFGKREDDNSGLGDWFVNEKKM
                     NGSMAQLAEKIHRMGMKFGLWFEPEMISEDSDLYRAHPDWAFAIPGRVPNHSRNQLVL
                     DMTREDVREYLLERLTTIVHDAKIDYVKWDMNRSVCDVYSHVAAQSRNGELYHRYVLG
                     VYDLMERFLAACPNLLLEGCSGGGGRYDAGMMYYSPQIWCSDNTDAINRLSIQYGSSF
                     FYPISTVGSHVSVCPNHQTGRVTPFRTRGDVALAGSFGYEM"
BASE COUNT          482 a          544 c          427 g          549 t
ORIGIN      
        1 catctcatat ccaaagcttc ctgccagcgc cacatcgcct cttgtccgga acggtgtcac
       61 acggccggtc tgatggttcg ggcatacaga aacgtgggaa ccaacggtac tgatcgggta
      121 gaagaacgag cttccgtact ggatagacag gcggttgatc gcatcggtat tatcgctgca
      181 ccagatctgc ggggaataat acatcatacc ggcatcatat cttccgcctc ctccgctgca
      241 gccctcgagc agcagattcg ggcatgcggc aaggaatctt tccatcaaat cgtaaacacc
      301 gagcacatag cggtgataca gctctccgtt ccggctctgt gctgccacat ggctgtaaac
      361 atcgcagacg ctgcggttca tatcccattt gacataatcg atctttgcat catgaacaat
      421 ggttgtcaga cgctccagca gatactcacg cacatcctca cgcgtcatat caagtacaag
      481 ctgatttctg ctgtggttcg gcacacggcc aggaattgca aatgcccaat ccggatgtgc
      541 acggtaaaga tcactgtcct cggaaatcat ctccggctca aaccagagac cgaatttcat
      601 gcccatccta tggatttttt ctgcaagctg cgccatggag ccgttcatct ttttctcgtt
      661 gacgaaccag tcaccgagac cactgttatc atcctctcgt tttccaaacc agccatcgtc
      721 aagcaccagc atctcaatgc caagcttttg cgcctgctct gcaattttca gaattttttc
      781 ttcgttgaaa tcaaaatagg ttgcttccca gttgttgatc agaatcggtc gtttggcatg
      841 cttgtatgcg ccgcggcaga tatgctcgcg gatgatctca tgatactgct gtgacagttt
      901 ggtaagaccg gttccggaat agcttaagat tgcctgcggg gtatcgaacg cctctccttt
      961 ttccagatgg aagcagaaat tggtcggatg aatacccatc tgaaaacggg tctgattttt
     1021 ctgatccttc tgtgctgccg caacaaagtt tccgctgtaa acgaggcatg cgccgatgca
     1081 ggaaccgtgt gtctcacccg caccctcctc gcagaggatc acagccggat tgtaatgatg
     1141 gctcgatgta ccacgggtgc ttccgatctc gacttttgca tgctgcaccg gaatccgctc
     1201 cgccgtacgc tccatcgcat gacgacccga gaaatagatc atctcataat tttcatacat
     1261 cagatccatg ctgaatgaca gcattttttc cagctcaatc gccgtttctc cgtgattttc
     1321 gagtcttgcc gctctcgtaa ttacattttc attttcaaat acaccgtaaa acagcttcag
     1381 cacaatatcg gaagccttct ccgtcattgt aataacaaga gtctcgcctt tttctttctc
     1441 tgtatcgtac agcgccggca ttcccggaat ggcataggaa tgatccagaa tttcgaacga
     1501 atcgaaacga aaatctgctg caaagctgcc gtccgcgtgg cggatatcaa tcatatcatc
     1561 acggaaatca ccgacaccac ttcccggaat ttcctgcgga agtgtatcaa gagaatattt
     1621 ccggttaggg cctgcttcct ccggatttcc tgcaaatccc atatccgtac agaaaatcag
     1681 accggaaaga ttttcctcac cgattttttc tccatagtag agatgcagaa gcacattttt
     1741 gtcatctgcg aacatctggt aactactgtc ttttgtctgg agggtaaata accgtccatt
     1801 ttcacttact ttgattgcca tggttttttc tcctttatct tatcgcattt tttcgggctg
     1861 ctcacaccaa aatcactttc ttacataccc gaacagcaag tgttccggtg ctgtgtgaac
     1921 cgcaaaggct gctactccgg gcctttgatt tcctacagcg gcttgcagta tcctgcaaaa
     1981 cacgatataa ttttactgat at
//
LOCUS       DAOMUF010000275         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_16286_length_1973_cov_2.877477, whole genome shotgun sequence.
ACCESSION   DAOMUF010000275 DAOMUF010000000
VERSION     DAOMUF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16286_length_1973_cov_2.877477"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="PLN77_10485"
     CDS             <1..1452
                     /locus_tag="PLN77_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRM44731.1"
                     /translation="ALERRFQPVKVEEPTESEALDILKGLRPYYERHHGVEITDEALE
                     AAVKMSVRYINDRFLPDKAIDLIDEASAGVQLAGYQVPDNMAKEEQTLFEVQKEKESA
                     ISAGDFEQAKELQARQQELEKDLEQIRKRFERRCKNKKLQVTEEEIAKTVSTWTKIPV
                     QRLAEGESKRLARLEQTLHKRVVGQEEAVTAVAKAVKRGRVGLKDPNRPTGSFLFLGP
                     TGVGKTELSKALAEAVFGTEQALIRVDMSEYMEKHSVSKLIGSPPGYVGYEEGGQLSE
                     KVRRNPYSVILFDEIEKAHPDVFNILLQVLDDGHITDAQGRKVDFKQTCIIMTSNAGA
                     QNIVAPKRLGFLSTEDERKDYEYMKGQVMEEVKRMFKPEFLNRIDEIIVFHQLTKADM
                     KKILQILLKDLERRCKEQMDIELKVRDTVKEYLVENSFDSKYGARPLKRAIQSKIEDP
                     LAEAILNGEVKKGDTVAAGMNKKEITFKVLEKK"
     gene            1556..1969
                     /locus_tag="PLN77_10490"
     CDS             1556..1969
                     /locus_tag="PLN77_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HRM44732.1"
                     /translation="MSVVKELIRTEADGGISFGDYELAAKAKKSDFEHRGDLYKVKTF
                     KEITKLERNGLFVYESVPGTAVTDLNVFDDGMTFKVEGPEDAQITVEMEAETEYKVLL
                     DGVNVGHMTTNLGGKLSFSVELEKAEDVAVEIVKA"
BASE COUNT          645 a          453 c          515 g          360 t
ORIGIN      
        1 gcattggagc gccgtttcca gccggtcaaa gtagaagaac cgacggaaag cgaagcactt
       61 gatatcttaa aaggcctgcg cccatactac gaacgccacc acggcgtgga aatcaccgat
      121 gaagcactgg aagcagccgt aaaaatgagc gtccgctaca tcaacgaccg tttcctgccg
      181 gataaagcca tcgatctgat cgatgaggca tccgcaggcg tacagctcgc cggctaccag
      241 gtgccggaca acatggcaaa agaggagcag acactgttcg aagtacagaa agaaaaagaa
      301 tccgcgatca gtgccggcga ttttgagcag gcaaaagaac tgcaggcgcg ccagcaggag
      361 ctcgaaaaag atctggagca gatccgaaaa cgctttgaac gccgttgcaa aaacaaaaaa
      421 ctgcaggtaa cagaagaaga aatcgcaaaa accgtatcca cctggacgaa aattccggta
      481 cagcgtctcg cggaaggaga atcgaaacgt ctggcaagac tggagcagac actccataaa
      541 cgcgttgtcg gccaggaaga agccgtgaca gctgtggcaa aagccgtaaa acgaggcaga
      601 gtcggattaa aagacccgaa ccgtccgaca ggatccttcc tgtttcttgg accgaccggt
      661 gtcggaaaaa cagaactttc caaagcactc gccgaggcag ttttcggtac agagcaggcc
      721 ctgatccgcg tcgacatgtc ggagtacatg gagaaacaca gcgtctccaa actgatcggc
      781 tcccctccgg gatacgtcgg ctacgaggaa ggcggccagc tctccgaaaa agtgcgccgc
      841 aacccgtaca gtgtgatcct gtttgacgag atcgaaaagg cgcacccgga tgtcttcaac
      901 attctgctgc aggtgcttga tgatggccat atcaccgacg cgcagggcag aaaagttgat
      961 ttcaaacaga cctgcattat catgacatcc aatgccggag cacagaacat tgtagcgccg
     1021 aaacgccttg gattcctttc gacagaagat gagcggaaag actacgaata catgaagggc
     1081 caggtgatgg aagaagtcaa acggatgttt aagccggaat tcctgaaccg aatcgacgag
     1141 attattgtat tccatcagct taccaaggcg gatatgaaga agattctgca gattctgctc
     1201 aaagatctgg agcgccgctg caaggagcag atggacattg aattgaaagt ccgtgacacc
     1261 gtgaaagaat atctcgtgga aaacagcttc gacagcaagt acggtgcccg cccgttaaaa
     1321 cgcgcgatcc agagcaaaat tgaggatccg ctcgcagaag ccatcctgaa cggagaagtg
     1381 aaaaaaggcg ataccgttgc ggctggaatg aataagaaag agattacatt taaggttctt
     1441 gagaaaaaat aatataaaat tccataaatt atatggtaaa atctttttga ttatggtatg
     1501 attttcacaa agaaacgaaa caccacaaaa gacaagacca ggagggcatt tacaaatgtc
     1561 agtagtaaaa gaattaatcc gcacagaggc agacggaggc atcagcttcg gagattatga
     1621 gcttgcggca aaggcaaaaa aatctgattt tgagcacaga ggagatctgt ataaagtaaa
     1681 aacctttaag gaaatcacaa agctggagag aaacggactg ttcgtatacg aatccgttcc
     1741 gggaaccgca gttaccgatc tgaacgtatt cgacgacggc atgaccttca aagtagaagg
     1801 accggaggat gcacagatca ccgttgaaat ggaagcagag accgaataca aggtgctgct
     1861 cgacggcgta aacgttggcc acatgaccac aaacctcggc ggaaaacttt ccttcagcgt
     1921 agagctggaa aaagcagagg atgtagcagt agaaatcgta aaagcatgat gaa
//
LOCUS       DAOMUF010000276         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_16483_length_1957_cov_2.182965, whole genome shotgun sequence.
ACCESSION   DAOMUF010000276 DAOMUF010000000
VERSION     DAOMUF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16483_length_1957_cov_2.182965"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PLN77_10495"
     CDS             complement(<1..1223)
                     /locus_tag="PLN77_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HRM44733.1"
                     /translation="MNLKKKYGNYELHPQMKITGYENEIWDEKKEIIEELKRAVQEKQ
                     KEKKTCILSFDLYPGVRKEEIMELANALQPDRIFDIEDCAKDEETLLREFNDYITDDR
                     VFGIMCHKAIDTWFESEKLETMKKAIETERAEEKDTNGGLIVIVGTAAELLTEADLLV
                     YCDLTRWEVQLRYRSGMPNWHSTNYNDPILTKYKRGFFIEWRLADRYKKERYEKFTYL
                     LDTEKENAPVLTTGNAFRGALQQLAGQPFRMEPYFDPGVWGGQWMKENFGLDASKENF
                     AWSFDGVPEENSLNLEIGGKVLKVPAQDLVFYAPHELLGERVHGRFGAEFPIRFDLLD
                     TMGGQNLSLQVHPLTEYIYEKFGMPYTQDESYYLLDADEDEETYVYLGLKEGVDKKEM
                     ERELRAAEKGEELFPA"
     gene            complement(1369..>1957)
                     /locus_tag="PLN77_10500"
     CDS             complement(1369..>1957)
                     /locus_tag="PLN77_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM44734.1"
                     /translation="ELSRVTVRQAMTNLVQSGYARARRGIGTDVVYEKVEEQMEGVIS
                     FTEEMKKHHIEMQTTYCKMELISPGETVARSLQIPLTEPCYCLKRVRNAVGKPMVYTI
                     TYLKKICELPTEPEPYMESLYQYLREEHGIYIESGRDTLEAALPSEEVQKALKIDAQM
                     PIFIRTRQTFRKGGEVFEYSICYYPGNRYKYTVEL"
BASE COUNT          415 a          525 c          388 g          629 t
ORIGIN      
        1 gcagggaaaa gttcttctcc tttttctgct gcacggagtt cccgctccat ctctttttta
       61 tccacgcctt ctttgaggcc gaggtataca tacgtttcct catcttcatc ggcatccaga
      121 agatagtagc tctcatcctg cgtatatggc atgccgaatt tttcatagat atattcggtc
      181 agcggatgta cctgcaggga aaggttctga ccgcccatcg tatccagcag gtcaaaacgg
      241 atcgggaatt ctgcgccgaa ccggccgtgg actctttcac ccagcagttc atgcggcgca
      301 tagaagacaa gatcctgtgc cggtactttt aagacttttc cgccgatttc cagattcagg
      361 ctgttttcct ccggaactcc gtcaaagctc cacgcgaaat tttccttcga tgcatccaga
      421 ccaaagtttt ccttcatcca ctgaccgccc catacgccgg gatcaaaata cggctccata
      481 cgaaatggct gtccggcaag ctgctgcagt gcgcctcgga aggcatttcc cgttgtaaga
      541 accggtgcat tttctttctc cgtatccaga agatacgtga atttttcata ccgctctttt
      601 ttatagcggt ccgccagacg ccattcaatg aaaaatcccc gtttatattt cgtcaggatc
      661 ggatcattat aattggtact gtgccagttt ggcatgccgc tccgatagcg gagctgaact
      721 tcccagcggg tcagatcgca gtaaacaagt agatctgcct cggtcagaag ctctgccgct
      781 gtaccaacga tcacgatcag accaccgttt gtatcttttt cttccgcacg ttctgtctca
      841 attgcttttt tcatggtttc cagcttttcg ctctcaaacc aggtatctat tgctttatga
      901 cacatgatac cgaaaacacg gtcatcggtg atgtaatcgt taaattctct taacaacgtt
      961 tcttcgtctt tggcacagtc ttcgatatca aaaatccggt ccggctgcag cgcattggcc
     1021 agttccatta tttcttcttt tcgtacaccc gggtacagat caaaggaaag aatacaggtt
     1081 ttcttttctt tctgtttttc ctgaaccgcc cgttttaatt cttcgatgat ctcctttttt
     1141 tcatcccaga tttcattttc gtatcctgtg atcttcattt gcggatgtaa ttcgtagttt
     1201 ccatatttct tttttaaatt catttggatt cttttccttt tcttaatttt tcttccatcc
     1261 attgggttgt ttcctgcgcc gatcagacct atcatctttg tttcaacgct ttcgcatctg
     1321 gcggatttcg cacttctgtc cggtttcgtt tttttacatg tcatatgttt acagttccac
     1381 cgtgtactta taacgatttc ccggataata gcagatcgaa tattcaaaaa cctcgccgcc
     1441 cttccggaat gtctgccgtg tccggataaa aataggcatc tgcgcatcaa ttttcagtgc
     1501 tttctgcacc tcttccgatg gaagtgccgc ctcaagcgta tcccggccgg attcaatata
     1561 gattccatgt tcctcccgca gatactgata cagcgattcc atgtatggct ccggctctgt
     1621 tggcagttcg cagatttttt tcagatacgt aatggtatag accatgggtt ttcccacagc
     1681 attcctcaca cgtttcaggc aataacatgg ctccgtcaat ggaatctgca gacttcttgc
     1741 taccgtttcc ccgggactga tcagttccat cttgcagtat gttgtctgca tttcgatatg
     1801 atgctttttc atttcttccg tgaaactgat gacaccttcc atctgctctt cgactttctc
     1861 gtagacaaca tctgttccga tgccgcgccg tgcacgggcg taaccgctct gtaccagatt
     1921 cgtcatcgcc tggcgtactg tcacacgcga aagctcg
//
LOCUS       DAOMUF010000277         1941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_16659_length_1941_cov_4.600212, whole genome shotgun sequence.
ACCESSION   DAOMUF010000277 DAOMUF010000000
VERSION     DAOMUF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16659_length_1941_cov_4.600212"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..288
                     /locus_tag="PLN77_10505"
                     /pseudo
     CDS             1..288
                     /locus_tag="PLN77_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
     gene            293..631
                     /locus_tag="PLN77_10510"
     CDS             293..631
                     /locus_tag="PLN77_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44735.1"
                     /translation="MADEITEEQYQAEVLDYEEAHRKLNKQLTAIMENTMVWERALSL
                     RNPWIQQMEQYKTPEALDRNFVKKYIEQVVVTFLGDRQAEISLTMKTEEWKQMLERIE
                     LEGTDDGTKK"
     gene            615..896
                     /locus_tag="PLN77_10515"
     CDS             615..896
                     /locus_tag="PLN77_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44736.1"
                     /translation="MARKSRKNLPQPEQAMVSVQFPELDEEKIPTAIYGRLSVEDDED
                     NESMETQIALVQDFINRSGELNYVDTYFDNRSQNFLFSPLAEMASEAKM"
     gene            946..1067
                     /locus_tag="PLN77_10520"
                     /pseudo
     CDS             946..1067
                     /locus_tag="PLN77_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
     gene            1388..>1941
                     /gene="pgk"
                     /locus_tag="PLN77_10525"
     CDS             1388..>1941
                     /gene="pgk"
                     /locus_tag="PLN77_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRM44737.1"
                     /translation="MGLGKKTIDDQNYCGKKVLVRCDFNVPMKDGVITNENRINAALP
                     TIQKLVNDGAKVILCSHLGKPKNGPEAKFSLAPVAVALSAKLGKTVVFADDDNVVGEN
                     AKAAVAAMNNGDVVLLQNTRFRKEETKNMPEFSEELASLADAYVDDAFGSCHRAHCST
                     AGVTDFIKDTAVGYLMEKEIKYLG"
BASE COUNT          581 a          373 c          527 g          460 t
ORIGIN      
        1 tggcgcggaa gcgttgcatc caagtttatt cgtggtggcg aaaaaaatgc cgtgaaatat
       61 gcggatgaaa tcattgttct aagcaaaggt gtgcagaagt atttcatgga aatatacggg
      121 agggaaacac attttatccc taatggtgtg aataggccag agattcggga agcaaaactg
      181 attacagata agtttgggct gaaaaaagac tcctacatat tgttcctcgg tcgtctggtg
      241 ccggagaagg ggattcgata tctggttgag gcattcaaga atgtcaagct atatggctga
      301 tgaaatcacg gaagaacaat atcaggctga agtgctggat tatgaagaag cccatcgaaa
      361 gctgaataaa cagcttacag caatcatgga aaatacaatg gtatgggaac gggcattgag
      421 tttgcgaaat ccgtggattc agcagatgga acaatataag accccagagg cactggatcg
      481 taattttgtg aaaaagtaca ttgaacaggt cgtggttaca tttttaggcg atagacaggc
      541 agaaatcagt ctaacgatga aaacagagga atggaagcag atgcttgaac ggatagaatt
      601 ggagggtaca gacgatggca cgaaaaagta gaaagaattt gccccaaccg gaacaggcaa
      661 tggtttcagt gcagttcccg gaattggatg aagaaaagat accaaccgca atctatggac
      721 ggctttcggt ggaagatgat gaagataacg aaagcatgga aacgcagatt gcactggtgc
      781 aggattttat taaccgtagc ggtgaattaa attatgtaga tacgtatttt gacaatagaa
      841 gtcagaattt tcttttttcc cctctagcgg agatggcttc tgaggccaaa atgtgaccca
      901 gtaggtcaca ttttaaggaa caagtgacct gctgggtcac agaaaaatta tctgatatcc
      961 atgaatatgt gggggggttg actccggatt ttctggatgt attggaggaa ggcttcaaag
     1021 aattctttga aaaagaagtg tcgaataatc ctctgaaagc agaatatttt caggaagtat
     1081 aaggcgagcg gctatgctgg tgtggaagca atttttgagc gggatcagcg gaaccagaag
     1141 ttcatatggg gaatgtatga ccgcatttgc ggaatgttct gccggaaaac acggtaaggc
     1201 tgcggctttc ggtcaaaata cacacatttc cgtgcatcag cggcaaatgt ttgttaaact
     1261 attatctatc ttgcgacctt gcaatgtgca cggtttttgt ttataatagt agagtaacgg
     1321 ggcaagatcc cccgaatgcg agcgaaactc tagttcagaa attaaaactt gagaggagtt
     1381 tattaccatg ggtttaggta agaagacgat cgacgatcag aactactgcg gcaagaaggt
     1441 gcttgtccgt tgcgatttca acgtcccgat gaaggacggt gtgatcacca acgagaatcg
     1501 catcaatgca gctctgccca ccatccagaa gctggtcaac gatggcgcaa aggtcatcct
     1561 gtgcagccat ctgggcaagc ccaagaacgg ccccgaggca aagttcagcc tggcacccgt
     1621 tgctgtggct ctgagcgcaa agctgggcaa gaccgttgtg tttgcagatg acgacaatgt
     1681 tgtgggcgag aacgcaaagg ctgctgttgc tgccatgaac aacggcgacg tcgttctgct
     1741 gcagaacacc cgtttccgca aggaagagac caagaacatg cctgagttca gcgaagagct
     1801 ggcttctctg gctgatgcat atgttgacga tgcattcggc agctgccacc gtgcacactg
     1861 ctccactgca ggtgtcaccg acttcatcaa ggataccgct gtcggctacc tgatggagaa
     1921 ggagatcaag tatctgggca a
//
LOCUS       DAOMUF010000278         1926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_16826_length_1926_cov_3.257616, whole genome shotgun sequence.
ACCESSION   DAOMUF010000278 DAOMUF010000000
VERSION     DAOMUF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16826_length_1926_cov_3.257616"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLN77_10530"
     CDS             complement(<1..697)
                     /locus_tag="PLN77_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HRM44738.1"
                     /translation="MDEKKKQASGPVKWILIGISVIFLFVMLVLPLVVVVTQSLSKGW
                     ECYVQAVTDSYTLKALALTIEATVAAVVCNTIFGLCAAWAMTRFHFRGKKLLTTLIDI
                     PVTVSPVIAGLIYILVFGKQSPLYSVLDKAGLQIVFAVPGIILATIFVTFPFVSREII
                     PVLESIGSDEEEAAALMGAKGWTIFTKVTFPHIKWAFLYGVVLCTARAMGEFGAVSVL
                     SGHLRGKTNTLPLH"
     gene            complement(697..1536)
                     /gene="cysT"
                     /locus_tag="PLN77_10535"
     CDS             complement(697..1536)
                     /gene="cysT"
                     /locus_tag="PLN77_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRM44739.1"
                     /translation="MKKQQSKRVIPGFGLTMGVTLAMLSIVVLIPLASLAVYSARLGF
                     REFIQVITRPRVLSGFYVSFITAFVATVVNAIMGLILAWVLVRYDFPGKRIMDGMIEL
                     PFALPTAVAGISLTSLTSDKGLVGSFFAKFGIKIAYTRIGITVALIFVGIPFVVRAIQ
                     PVLEKLDGSYEEAARVMGAKPFTIFRRVILPELLPAMLSGAGLAFGRCLGEYGSVVFI
                     AGNKPYYTEITPLIIMSKLQEFDYESATSIALVTLAVSFLILFGVNLVQARNTKRIRG
                     RDA"
     gene            complement(1574..>1926)
                     /locus_tag="PLN77_10540"
     CDS             complement(1574..>1926)
                     /locus_tag="PLN77_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM44740.1"
                     /translation="NEAFLSVRDNPDDYEIVTPSISILAQPSVAVVDENVKKHDNADA
                     AKAYLKYLYSDEAQELIAENYYRPVNQTILKKHADTFNLSVKLTTIKDFGGWDAVQKK
                     HFVDGGVFDEIYEE"
BASE COUNT          528 a          534 c          383 g          481 t
ORIGIN      
        1 catgaagcgg aagtgtattt gtttttccac ggagatgtcc ggacagtacg gacacggctc
       61 caaactctcc catagctctc gctgtacaca gcacaacgcc ataaaggaat gcccacttga
      121 tatgcgggaa agtcactttg gtaaagatcg tccagccttt tgctcccatc agtgctgccg
      181 cctcttcctc atcggaccct atagattcca gcaccggaat gatctctctt gatacaaatg
      241 gaaatgtaac aaaaatcgtt gccaggataa ttccaggaac tgcaaacact atctggagcc
      301 ctgctttgtc aagaacagaa taaagcgggc tctgctttcc aaaaaccaga atataaataa
      361 gacctgcgat gaccggtgac accgtaaccg gaatgtcgat cagcgtcgtc agaagctttt
      421 tcccccggaa atgaaatctg gtcatagccc atgctgcaca taatccgaag atcgtgttgc
      481 atacaacagc agccaccgtt gcctcaatag tcagcgccag agctttcagc gtataagaat
      541 ccgtcacagc ctgcacatag cactcccaac ctttactcag tgactgggtc acaacaacga
      601 ccaacggaag caccagcatc acaaaaagaa agatcacgga aatcccgata aggatccatt
      661 tcaccggccc ggaagcctgc ttcttttttt catccattat gcatccctcc ctctgatacg
      721 tttcgtattt cttgcctgca ccagattcac gccaaacagg atcaaaaacg ataccgccag
      781 tgtaaccagc gcgatagatg ttgcactttc gtagtcaaac tcctgaagtt ttgacatgat
      841 gatcagcggc gtgatctctg tataataagg tttgtttcct gcgataaaaa caacacttcc
      901 gtactctccc aggcagcgtc cgaaggcaag tcccgctccg gaaagcattg ccggaagaag
      961 ctctggaagg ataaccctcc ggaaaatggt gaatggtttt gcccccatga cacgtgctgc
     1021 ctcttcatag gaaccatcca gtttttccag caccggctga atagcccgga ctacaaacgg
     1081 aattcccaca aagatcagag ccaccgtgat accgatcctg gtatacgcga tcttgatccc
     1141 gaactttgca aaaaaacttc ccacaagccc tttgtcggat gtcagtgatg tcagcgagat
     1201 accggcaact gcagttggca gcgcaaaagg cagctcgatc attccatcca tgatcctttt
     1261 tcccggaaaa tcataccgca ccagcaccca cgcaaggatc agtcccatga tggcattcac
     1321 cacagtggcc acaaatgcgg taataaaact tacgtaaaat ccggacaaaa ctcgaggtct
     1381 cgtgatcacc tggataaatt cccgaaatcc cagccgcgcc gaataaactg ccagagatgc
     1441 aaggggaatc agaaccacga tactcagcat cgccaaagtc actcccattg tcagcccaaa
     1501 gcctggtatc acccgtttac tctgttgttt cttcatttct gtctcccctc ttttctgtat
     1561 ccgctatgct tttttactct tcataaatct catcaaacac tccaccgtcc acaaaatgtt
     1621 tcttctgtac tgcatcccat ccgccgaaat ctttgatagt agtcagcttc acactcaggt
     1681 taaaagtatc tgcatgtttt ttcaggatgg tctgattgac cggtctgtaa taattctcag
     1741 cgatcagctc ctgcgcttca tcggaataca gatatttcag ataagctttt gcggcatctg
     1801 cgttatcgtg tttttttaca ttttcatcta caacagcaac ggacggctgt gccaggatac
     1861 tgatgctcgg tgttacgatc tcgtaatcat ccggattgtc ccttacagaa agaaacgctt
     1921 catttt
//
LOCUS       DAOMUF010000279         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_16930_length_1917_cov_2.646079, whole genome shotgun sequence.
ACCESSION   DAOMUF010000279 DAOMUF010000000
VERSION     DAOMUF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16930_length_1917_cov_2.646079"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLN77_10545"
     CDS             <1..348
                     /locus_tag="PLN77_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44741.1"
                     /translation="SGPAERSLYFTENPDFTAPSAGIHSRRFPGSRIFLYSSDPEHDL
                     AAVPSSLFDVLHRYFAVHSPDDVVLWKDGILSFQTMENQPQPLTALCTPDDARDSKII
                     PFPTLSERQRISQ"
     gene            complement(413..1354)
                     /locus_tag="PLN77_10550"
     CDS             complement(413..1354)
                     /locus_tag="PLN77_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM44742.1"
                     /translation="MIKIDLITGFLGSGKTTFLKQYAKYLMEQGKNIGILENDFGVVN
                     VDMLLLQELRGDQCELEMISGGCDKETHRRRFRTKLISMGMSGYDRILVEPSGIYDVD
                     EFFDTLYDEPLNRWYEAGSVITILDAGLDEKLSEEEEYLLASEAANAGKIVLSKVQNV
                     SEEKKEETIAHLNRTLEQAGCRRQFSDAEILQKNWDDLTEDDFKMLSECSYRSEDYRK
                     LDFGEQQTFDSLCFLEPKITEEALKKAAEAIFADPSCGNVFRIKGIVKTGETVWSEIN
                     ATREQMTFQAVPESQEVLIVIGAGLSKERISGILGIK"
     gene            complement(1411..>1917)
                     /locus_tag="PLN77_10555"
     CDS             complement(1411..>1917)
                     /locus_tag="PLN77_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRM44743.1"
                     /translation="VIAMREMRLKKREITDPEILREILESCEVVRIGAMDEEGMFIVP
                     MNYGYDVREEDGTLHVCLYLHGAKEGRKAAAFAADPRVAVEMDYNHEVITGDYTCAYS
                     YAYRSIMGNGTITEVTDPEVKKKGLEKIMAHIAPEAKIAFLPEMVERVAVWRIDIDTF
                     TGKERRRK"
BASE COUNT          405 a          593 c          374 g          545 t
ORIGIN      
        1 tccggacctg cagaaagatc cttatatttt accgaaaacc cggattttac cgctccctct
       61 gccggtatcc attcccgtcg ttttccggga agccggatct ttctatattc cagcgatccc
      121 gagcacgatc ttgcggcagt tccatcctct cttttcgatg tgctgcaccg ctattttgcc
      181 gtccattcac cggatgatgt cgttttatgg aaagatggca ttctctcctt ccagaccatg
      241 gaaaatcagc cgcagccgtt gactgctctt tgtacaccgg atgatgcacg ggattccaaa
      301 atcattccgt tccccacact gtcagaacgg cagcgcattt cacagtaact gctatttagc
      361 caaaccagca ggagcatgat ggaaaatacc cctttccgtc atgctccatt ttttacttaa
      421 ttccgagaat tccactaatc cgctctttcg aaagccccgc gccgattaca atcagcactt
      481 cctggctctc cggcactgcc tggaatgtca tctgttcccg cgtcgcattg atttcgctcc
      541 acacagtttc gcccgtcttt acaattcctt tgatccggaa tacattgccg catgatggat
      601 ccgcaaagat tgcttctgct gcctttttca gcgcttcttc cgtaatcttc ggctccagaa
      661 agcagagcga atcaaatgtc tgctgctcgc cgaaatccag cttccggtaa tcctccgaac
      721 ggtaactgca ttcggagagc attttgaaat catcctctgt cagatcatcc cagtttttct
      781 gtaaaatttc tgcatcagaa aactgtcttc tgcagcctgc ctgctcgagc gtgcggttca
      841 gatgggcaat cgtttcttct ttcttttcct ccgaaacatt ctgtactttg ctcagcacga
      901 ttttaccagc gttcgccgcc tcggaagcca gcagatactc ttcttcctcc gacagcttct
      961 catcgagccc ggcatccaga atcgtgatga cgcttcccgc ctcataccag cggttcagcg
     1021 gctcgtcgta cagcgtgtca aagaactcat ccacatcgta aatccccgac ggttccacga
     1081 gaatgcggtc gtagccgctc atgcccatcg agatcagttt cgtgcggaac cgtctgcggt
     1141 gtgtctcttt gtcgcatccg ccggaaatca tctccagctc gcactgatcg ccgcgcagtt
     1201 cctgcagaag cagcatatcc acattgacta ccccaaaatc attctccagg atgccgatgt
     1261 tctttccctg ttccatcaga tactttgcgt actgtttcaa aaaagtcgtc ttccctgagc
     1321 caaggaagcc tgtaatcaga tcaattttaa tcatttctgc tgtcctttct gctgtctttt
     1381 tccgtctgcg cctgcggagc ttcgaacact ctattttcgt ctgcgctctt tgccggtaaa
     1441 tgtatcgatg tctatgcgcc atacggcaac gcgctccacc atctccggca gaaaagcgat
     1501 cttcgcctcc ggtgcgatat gcgccatgat tttttccagg cctttcttct ttacctccgg
     1561 atctgtcacc tccgtgatgg ttccgtttcc catgatactg cggtacgcat aggaatacgc
     1621 acacgtatag tcacctgtaa tcacttcatg gttgtaatcc atctccaccg ccacgcgcgg
     1681 atccgctgca aatgcagccg ccttccgccc ttcttttgcc ccatgcaggt acagacagac
     1741 gtgaagagtt ccatcttctt cccggacatc atatccataa ttcatcggca cgataaacat
     1801 gccttcctca tccatcgccc cgatccgcac aacctcgcag ctttccagaa tttctcttaa
     1861 aatttcagga tctgtaatct cccgcttttt taaacgcatt tcacgcatcg caatcac
//
LOCUS       DAOMUF010000280         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_16968_length_1914_cov_2.256052, whole genome shotgun sequence.
ACCESSION   DAOMUF010000280 DAOMUF010000000
VERSION     DAOMUF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16968_length_1914_cov_2.256052"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..893)
                     /gene="yqeC"
                     /locus_tag="PLN77_10560"
     CDS             complement(108..893)
                     /gene="yqeC"
                     /locus_tag="PLN77_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HRM44744.1"
                     /translation="METAFPFLLEPDHVVSLLGGGGKTTLLYEMAGFGVRNGQNVLVT
                     TSTHLYRPPEEWRDRTLKEVERKFQAGCAAIIGSDCRDPKKIAMPEEQLFETARKKAD
                     LTLIEADGTKHLPCKVPAEHEPALLSCSDLVVGVTGLSALGQPLEKSCFRAKLAAERL
                     GCPMDHRITEEDLAQLIAAEWGQQKDLDGRAFYAVLHQYEEKEHKKAVRKIAELLQRR
                     GIRQIFVTCVPKNQMTGEGSMACSWHGRADTLQREKSGAKEPE"
     gene            complement(1083..1838)
                     /locus_tag="PLN77_10565"
     CDS             complement(1083..1838)
                     /locus_tag="PLN77_10565"
                     /inference="COORDINATES: protein motif:HMM:NF019161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRM44745.1"
                     /translation="MKKGILAGLIVLCLALFAGTAAVMVTGDRKGPEIAVPSGADVTY
                     EEGSDTALLLEGVTATDDRDGDVTDSVIIENIFPNDDRTGASVIYAAKDSHNNVTKVT
                     RRVNYKAAGDAAAPEENTETDGSDAAPAENAEAEGSDAAPAAEEAQQNDTEGVKNEAN
                     AKMEIEALPSKSPRMYLNTYETTGKAGSELDKISYIKEITDDADSQKTLFAEIQVDGE
                     VDTATPGDYTLTYHVTDSNGNQSNMAVLTVHVQ"
BASE COUNT          361 a          603 c          387 g          563 t
ORIGIN      
        1 ggacatccac catgacggcg ctgccgtcct tcttaaaatg agtaaattct gccatttttc
       61 tcctccgttt ttttcctgta cgctccggat ctttctctct ggatctttca ctccggctct
      121 ttcgctccgg atttttctct ctggagcgtg tctgcccttc catgccagga acacgccatg
      181 ctgccttctc ccgtcatctg gttctttggc acacaggtga cgaaaatttg tcggattcca
      241 cgcctctgca gcagctccgc aattttccga accgcctttt tatgctcctt ttcctcatac
      301 tgatgaagaa ccgcataaaa agctctgccg tccagatctt tttgctgtcc ccattctgct
      361 gcgatcaact gtgccagatc ctcttccgtg atgcggtgat ccataggaca tccaagccgt
      421 tcggccgcaa gcttggcccg aaaacagctc ttttcaagcg gctgtccaag cgccgaaagt
      481 cccgtcacgc cgaccacaag atcggaacag gaaagcagcg ccggctcatg ctctgccggc
      541 accttgcacg gcagatgttt tgtcccgtcc gcctcgatca gggtcagatc tgcttttttc
      601 cgtgcagtct caaacagctg ctcctccggc atcgctatct tttttggatc ccgacagtca
      661 cttccgataa tcgccgcaca gcctgcctga aattttcttt ccacctcttt tagggtgcga
      721 tcccgccatt cttccggtgg gcggtaaaga tgcgtcgagg ttgtaaccag cacattctgt
      781 ccgtttcgga caccaaagcc tgccatctca tacagcagcg tcgttttccc accgcctcca
      841 agcagcgaaa ccacatggtc tggttccagc aaaaatggaa acgccgtctc catttccaca
      901 ggcaccagcc tctgcctttc tgtcgctttc cagattcgaa gcatcctttt ggcccccttc
      961 tttactttct tttcttttta ctttctcact ctaccatact cattttttgc tgtcaaattt
     1021 tcccctggca aagcgaaaaa aactgccccg acaggatgtt tcccatcaga gcagttctgc
     1081 ttttactgaa catgtacggt aagaaccgcc atatttgatt gatttccatt gctgtccgtc
     1141 acatggtagg tcagggtata atctcccggt gtcgcggtat ctacctcgcc gtccacctga
     1201 atctccgcaa aaagcgtctt ctgactatct gcgtcgtccg taatctcttt gatgtagctg
     1261 attttgtcca gctcacttcc ggctttcccc gtcgtctcgt acgtattcag atacattctc
     1321 gggcttttgg atggcagtgc ttctatttcc attttggcat ttgcctcatt tttcacaccc
     1381 tcggtatcat tctgctgtgc ttcttccgcc gccggagcag catcgctacc ctctgcttct
     1441 gcgttttccg ccggtgcggc atcactgcca tctgtttccg tgttttcctc cggcgcagcc
     1501 gcatcccctg ccgctttata attcacgcgg cgtgtcacct tcgtcacatt gttgtggctg
     1561 tcctttgcag catagatcac cgatgcaccg gtacggtcat catttggaaa aatattttcg
     1621 atgataacag aatcagtcac atccccgtct ctgtcatcgg ttgccgtgac gccttccagc
     1681 agcaaagccg tatcgcttcc ctcctcgtac gtcacatccg caccggacgg aaccgcgatt
     1741 tccggtcctt ttcggtcacc cgttaccatc actgcagctg taccggcaaa aagagccaga
     1801 cacaggacaa tcagacctgc aagaattcct tttttcatgg ctgttactcg tcctcctcct
     1861 ggccgtactc gccataatac ttatactggc catacttgcc gtatttacca tatg
//
LOCUS       DAOMUF010000281         1901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_17117_length_1901_cov_2.638678, whole genome shotgun sequence.
ACCESSION   DAOMUF010000281 DAOMUF010000000
VERSION     DAOMUF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17117_length_1901_cov_2.638678"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLN77_10570"
     CDS             complement(<1..654)
                     /locus_tag="PLN77_10570"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HRM44746.1"
                     /translation="MGPQEMLDRAEVKNKWQLTPLKDFKSPRLTERMWRTAVVVLLTS
                     LIYEYLIPGGRNPCFALIGAVYGVGSRFEEGFHNGINRLIGTFVGGILVIPFYMLYQK
                     QPFGIPAFLWLAAGLCLVLWCNLALQADSAIQPGIVVYFVVLYTVGEERVVFYTLARI
                     LDTGIGVLISLLCYLLLPSKYDKEQGLSFRTMWKATKHAFQSYKDKNRLFREDEQKNF
                     "
     gene            complement(692..1324)
                     /gene="ttdB"
                     /locus_tag="PLN77_10575"
     CDS             complement(692..1324)
                     /gene="ttdB"
                     /locus_tag="PLN77_10575"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HRM44747.1"
                     /translation="MIEIRGDKKVLITPVSAEDLKDIHIGDIVWLDGDLMTCRDVAHR
                     RLVEYGRELPYNIKDKAIFHAGPIVRKIEGTEDDYEMVSVGPTTSMRMEKFEYEFTKL
                     TGVRVIVGKGGMGPNTERACKEFGAIHCVFPAGCAVVAATEVERIAEHHWDELGMPET
                     LWCNKVKEFGPLIVSIDAEGRNLFEENKVVFNERKEAAKQAIYPEVKFIK"
     gene            complement(1340..>1901)
                     /locus_tag="PLN77_10580"
     CDS             complement(1340..>1901)
                     /locus_tag="PLN77_10580"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRM44748.1"
                     /translation="EYNTKKNVGKGTPTVWWDIVPNSDKCEIYAYMAGGGCTLPGKAM
                     VLMPGAGYEGITDFVLDQMTSYGLNACPPLLVGVGVGTSIETAALNSKKALMRPIGSH
                     NENANAAKMERLLEDGINAIGLGPQGMGGKYSVMGVNIENTARHPSTIGVAVNVGCWS
                     HRRGHIVVNPDLTVTCDTHSTWKFNA"
BASE COUNT          452 a          520 c          415 g          514 t
ORIGIN      
        1 aaagtttttc tgttcatcct cgcggaacag acggttttta tccttataag actgaaacgc
       61 atgttttgtc gccttccaca tggtccggaa cgagagcccc tgttctttat catatttcga
      121 tggcagtaaa agatagcaga gaagggaaat cagtacgccg atgccggtat ccagaatccg
      181 ggcaagcgta tagaaaacaa ctcgttcctc accaactgtg taaagtacca caaagtagac
      241 cacgattccc ggctggatcg cagagtccgc ctgcagtgcc agattgcacc acaataccag
      301 gcagagcccc gccgccagcc acagaaaagc cggaattcca aacggctgtt tctgatacag
      361 catgtagaat ggaatgacca ggattcctcc cacaaacgtt ccgatcaggc gattgattcc
      421 attgtggaat ccctcttcaa accggctccc aaccccgtac actgcgccga tcagcgcaaa
      481 gcaggggttg cggccgccgg gaatcagata ttcataaatc agggaagtca gcagaacaac
      541 caccgctgtc cgccacatgc gttccgtcag acggggtgat ttgaaatcct tcagcggcgt
      601 gagctgccac ttatttttca cctccgcccg gtccagcatt tcctgtggtc ccataggctg
      661 ctcctgtcaa aaactcttat tatttgataa attatttgat aaacttcact tccggataaa
      721 ttgcctgttt tgcagcctct ttgcgctcgt tgaaaacaac tttgttctct tcaaacaggt
      781 tgcgtccttc tgcatcaata gaaacgatca gcggaccgaa ctcttttact ttattgcacc
      841 ataacgtctc cggcattccc agttcatccc agtgatgctc tgcaattctt tccacctcag
      901 tcgcggcaac aaccgcgcag cctgccggga agacacagtg aatggcaccg aattctttac
      961 atgcacgctc ggtattcgga cccatgccgc cttttccgac aatgacacgt acgccggtca
     1021 gttttgtaaa ttcatactcg aatttctcca tacgcatgga agtagtcggt ccgacagaaa
     1081 ccatctcgta atcatcttct gttccctcaa ttttccgtac gatcgggccg gcatggaaaa
     1141 ttgccttgtc tttgatatta tatggaagct ctcttccgta ctcaacgagt ctgcggtgtg
     1201 caacgtcacg gcaggtcatg agatcgccgt ccagccaaac aatatctccg atgtggatat
     1261 ctttcaggtc ttctgcgctt accggtgtaa tcagaacttt tttatctcct ctaatttcga
     1321 tcataattcg tacctccgtt tatgcattaa atttccaggt ggaatgtgta tcacaagtta
     1381 ctgtcagatc aggatttacc acgatatggc cgcggcggtg ggaccagcat cccacattta
     1441 cggcaacgcc gatcgtggat ggatggcggg cggtgttttc aatgtttaca cccataacgg
     1501 aatattttcc gcccattccc tgcggtccaa ggccgatggc attgatgccg tcttccagca
     1561 gacgctccat ttttgctgca tttgcatttt cattgtggga accgatcggg cgcatcagcg
     1621 ctttctttga atttaatgct gcggtttcga tggatgttcc aactccaacg ccgacaagaa
     1681 gcggaggaca cgcatttaag ccgtagcttg tcatctgatc cagtacgaaa tcggtgattc
     1741 cttcatatcc tgcacctggc atcagaacca tcgcttttcc aggaagcgta catccgccgc
     1801 ctgccatgta agcgtagatt tcgcatttgt cactgttcgg aacgatatcc caccatacgg
     1861 tcggggttcc ttttcctacg tttttcttgg tattgtactc a
//
LOCUS       DAOMUF010000282         1873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_17436_length_1873_cov_6.371837, whole genome shotgun sequence.
ACCESSION   DAOMUF010000282 DAOMUF010000000
VERSION     DAOMUF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17436_length_1873_cov_6.371837"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLN77_10585"
     CDS             complement(<1..727)
                     /locus_tag="PLN77_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRM44749.1"
                     /translation="MTACAIAPDKNFIQILETLSEKLEQPFFITYTPYTFKCILIFTA
                     AYFLGIGIYESQKRNYRRGVEHGSAKWGNVSEICRRYCEKQYTQNLLLTQHFRMGLDG
                     YKHKRNLNVLVVGGSGAGKSRTYAIPNIMQCNCSMVITDPKAELLRKTGGVLERNGYE
                     VRVFDLINPETSWCYNPFAYVWDDKDVLKLINNLIRNTTPKGAQSSDPFWEKSETALL
                     QALMLYLLHEAPPEEQNFPMIMEM"
     gene            complement(793..1071)
                     /locus_tag="PLN77_10590"
     CDS             complement(793..1071)
                     /locus_tag="PLN77_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HRM44750.1"
                     /translation="MVIYSREKVARLREQYPAGTRICLDSMDGEAGMPQGLEGTVQYV
                     DDAGQLGVKWDNGRSLSLIPGVDSFHKKEGQGHPEEKPRKKRESDLQR"
     gene            complement(1088..1399)
                     /locus_tag="PLN77_10595"
     CDS             complement(1088..1399)
                     /locus_tag="PLN77_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44751.1"
                     /translation="MTKYYLKMDNIMKNMFVVALNDELQAQSEQDECLEETKALLKKV
                     SEYDLAQKRLQITAGEQRQIRKALNRLRSKYLAAGRYSDGIDKVILQVAKPNTTRHMF
                     W"
     gene            complement(1483..>1873)
                     /locus_tag="PLN77_10600"
     CDS             complement(1483..>1873)
                     /locus_tag="PLN77_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44752.1"
                     /translation="NRELACAALDQHTGTYSRQKVSAQAYCAAYVIAKRYGVDVTGFN
                     LEYIAQRNECGKKDPQELRDFLNDVRQASYSIRNHIERNFGAKEQEYITDEFAIGDPV
                     KIPETKDEKPSEKASGKEKKAKSQPER"
BASE COUNT          419 a          486 c          353 g          615 t
ORIGIN      
        1 gcatttccat gatcatcggg aagttctgtt cttctggtgg agcttcatgc agcaaataca
       61 gcataagtgc ctgtaaaagt gcagtctcgg acttctccca gaacgggtct gaactctgcg
      121 ctcccttcgg tgttgtgttg cggatcaggt tattgatcag cttcagtaca tccttatcat
      181 cccagacata cgcaaacgga ttataacacc aggaagtctc cggattgatc aaatcaaaaa
      241 cccggacttc atatccattc cgttccagaa cacctccggt tttacgcaaa agttctgcct
      301 tgggatcagt aatcaccatc gaacagttgc actgcatgat gtttgggatt gcatacgtcc
      361 ggctctttcc agctcctgaa cctccgacta ccagcacatt taggtttcgt ttgtgcttat
      421 agccgtccag tcccatacgg aaatgctgtg ttaaaagcag attttgcgtg tattgttttt
      481 cgcagtaacg tctgcagatt tcggacacgt tgccccattt tgcggaacca tgttccacac
      541 ctcttctata atttcttttt tgggattcat aaataccaat gcctaagaaa taggctgctg
      601 tgaatatcag aatacatttg aacgtatacg gtgtatacgt gataaaaaac ggctgttcga
      661 gtttttcgct cagcgtttct aagatttgaa taaaattctt atccggtgca atggcacaag
      721 cggtaatcgc tgccgcccac cagacaaatg gcagaaagac aagatacaga acccagtcct
      781 gcttttttgt acttatctct gcaagtcaga ttctcttttc ttgcgtggct tttcttctgg
      841 atgtccctgt ccttctttct tatgaaagga atccactccc ggtatcagtg agagacttct
      901 gccgttgtcc cacttaacac caagctggcc tgcatcatcc acatactgca cagttccttc
      961 cagtccctgc ggcatacccg cttctccgtc catcgaatcc agacagattc ttgttcctgc
     1021 cggatattgt tctctaagtc gtgccacttt ttctcttgaa taaatgacca atcaaccact
     1081 cctttctcta ccagaacatg tgtctggtgg tattcggttt tgcaacctga aggatgactt
     1141 tatctattcc atcactgtat cttccggctg caagatattt acttctgagt ctgttcagtg
     1201 ccttacggat ctgcctttgt tccccggctg taatctgcag ccgtttctgg gcgaggtcgt
     1261 attcacttac ttttttcaga agagctttcg tttcttccaa acattcatcc tgctcactct
     1321 gtgcctgcag ctcatcattc agtgccacca cgaacatatt tttcataatg ttatccatct
     1381 tcaaatagta ttttgtcatc tggctgacct cctttttttg ttacaccaac catatcacaa
     1441 gacctccgta atagctactc caaccgtcca actttttcac ttttatcgtt ccggctggct
     1501 ctttgctttt ttctcttttc cggatgcttt ttcagatggt ttttcatcct tggtttccgg
     1561 tatttttaca gggtctccga ttgcgaactc atcggttata tactcctgtt cctttgcacc
     1621 aaaattgcgt tcgatatggt ttcgtatact gtaggaagcc tgacggacat catttaaaaa
     1681 gtctctcagt tcctgcggat ctttctttcc acattcattt ctctgtgcaa tatactccag
     1741 attgaatccg gtaacatcca caccataacg ttttgcaatc acataggccg cacaatatgc
     1801 ctgtgcgctg actttctgtc ggctgtaggt tccggtatgc tggtccagtg ctgcacatgc
     1861 cagttctctg ttc
//
LOCUS       DAOMUF010000283         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_17698_length_1852_cov_2.061770, whole genome shotgun sequence.
ACCESSION   DAOMUF010000283 DAOMUF010000000
VERSION     DAOMUF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17698_length_1852_cov_2.061770"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PLN77_10605"
     CDS             complement(<1..1061)
                     /locus_tag="PLN77_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM44753.1"
                     /translation="MIKTRLVGLLSHAKKYIVYTILWQWAALLSQVIAVFTIADLLER
                     VVYGAVTAAAVKRTIFTLAIVVVIRFFCERMGARSSYLACVDVKRILREKIYEKMLKL
                     GASYSEQVSSSEVVQVSTEGVEQLETYFGKYLPQLFYSLLAPLTLFAILCRVSMKASV
                     VLLICVPLIPVSIVVVQKIAKKLLNKYWSIYTGLGDSFLENLQGLTTLKIYQADQQKA
                     DEMDVESQNFRRITMKVLTMQLNSTSVMDIVAYGGAAVGMAVAVSEFLKGNLTVSGTL
                     CIVLLASEFFLPLRLLGSFFHIAMNGMAASDKIFKILDLPEPQTGERTLPDGPLDVVL
                     EDVHFSYEEDREILKGIDLT"
     gene            complement(1075..1422)
                     /locus_tag="PLN77_10610"
     CDS             complement(1075..1422)
                     /locus_tag="PLN77_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRM44754.1"
                     /translation="MNKVEKKRQKEQYVVEEMIRLYCRKNHGKEGHASGGMCPECQKL
                     ADYAKQRSQKCPFMEQKTFCANCKVHCYKPEMREKIRAVMRFSGPRMLLYHPILAIWH
                     LICSMREKRKAKA"
     gene            complement(1419..1823)
                     /locus_tag="PLN77_10615"
     CDS             complement(1419..1823)
                     /locus_tag="PLN77_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HRM44755.1"
                     /translation="MKSPFKIFWIVLGFLCLGFGTIGIVLPILPTVPFYMATLFCFAK
                     SSERLHSWFIGTQLYKKHLDSFVQHRAMTVGTKCKIIGMVTVVMAIGFFAMKNVPIGR
                     ICIAVVWMCHILYFFLRVKTVKTEDHILGETK"
BASE COUNT          449 a          534 c          396 g          473 t
ORIGIN      
        1 gtaaggtcga tgcccttcag aatttcccgg tcttcttcat aggaaaaatg tacatcctcc
       61 aacacgacat ccagcggtcc gtccggcagc gtccgctctc ccgtctgcgg ctccggcaga
      121 tcgaggattt tgaaaatctt gtcactcgcc gccatgccgt tcatcgcgat gtggaagaat
      181 gatccgagca gacgaagcgg caggaaaaac tcgctcgcca gcagcacaat gcagagcgtg
      241 ccggacacgg tgaggtttcc tttcagaaat tccgataccg ccaccgccat gccaaccgcc
      301 gcgccgccgt atgctacgat gtccatcacg ctcgttgagt tgagctgcat cgtcagaact
      361 ttcatcgtga tccggcggaa gttctgggat tccacatcca tctcgtcggc tttctgctgg
      421 tcggcctgat agatttttaa ggtcgtcagg ccttgcagat tttcaaggaa cgaatcgccg
      481 agccctgtgt aaatactcca atatttgttc agcagcttct tcgcaatctt ctgcacaacc
      541 acaatcgaaa ctgggatcag cggcacgcag atcagaagga ccacgctcgc tttcatgctc
      601 acccggcaga ggatggcgaa cagcgtgagc ggtgcgagca ggctgtaaaa cagctgcggc
      661 agatactttc caaagtatgt ttcgagctgc tccacacctt cggtggacac ctgtacgacc
      721 tctgaggaag acacctgctc gctgtacgat gcccccagtt tcagcatttt ctcataaatt
      781 ttttcacgca gaatccgttt cacatccaca cacgcaagat acgacgaccg cgcgcccatc
      841 cgctcgcaga aaaagcggat cacaacaaca attgccagtg taaaaatcgt ccgtttcaca
      901 gccgctgccg ttaccgcacc atacacgacc cgttccagca gatcggcgat cgtaaatacc
      961 gcgatcacct gcgacagaag cgccgcccac tgccacagaa tcgtgtacac gatatacttt
     1021 ttcgcgtggg acagcagccc caccagtctt gttttaatca taagtacctc tcttttatgc
     1081 ttttgctttt cgcttctctc tcatgctgca gattaaatgc cagatagcta aaatcggatg
     1141 atacagcagc atcctcggcc ccgaaaaccg cataacggcg cggatctttt cccgcatttc
     1201 cggcttgtag cagtgtactt tgcagtttgc gcagaatgtt ttctgttcca tgaagggaca
     1261 tttctggctc cgctgttttg catagtccgc taatttctga cattccggac acatcccgcc
     1321 ggatgcatgc ccctcttttc catggttctt ccggcagtac aggcggatca tctcttccac
     1381 gacgtactgt tctttctgcc gctttttttc tactttgttc attttgtttc tcctaggata
     1441 tgatcttctg tttttacggt tttgacccgc aggaagaaat acaaaatatg gcacatccat
     1501 accacagcaa tacagattct tccaatcggc acattcttca tagcaaaaaa gccgatcgcc
     1561 atcacaaccg ttaccatccc gataatttta catttcgttc ctaccgtcat cgcccgatgc
     1621 tgtacgaagc tgtcgagatg cttcttatat aattgagttc caataaacca gctgtgcagc
     1681 cgttccgaac ttttcgcaaa gcagaacagg gtcgccatat agaatggaac tgtcggcaga
     1741 atcggaagca caatgccgat ggtgccaaat cccaggcaca gaaaaccgag tacgatccaa
     1801 aatattttaa atggactctt cacaattttc ctcctttctc accgaatatt aa
//
LOCUS       DAOMUF010000284         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_18215_length_1813_cov_3.294084, whole genome shotgun sequence.
ACCESSION   DAOMUF010000284 DAOMUF010000000
VERSION     DAOMUF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18215_length_1813_cov_3.294084"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..512
                     /locus_tag="PLN77_10620"
     CDS             96..512
                     /locus_tag="PLN77_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM44756.1"
                     /translation="MYSMKEACIQTGMSYETLKFYCKEGLVPNVKRDSHNYRVFDDRD
                     IKWIQSLGCLKRCGMTLAEMKEYLAYCLEGPSSIPERKEILAVKKEALLASMEKLQKA
                     IDYIDWKQNFYDDVLSGKIEYHSNLISDEEIAQKAQ"
     gene            complement(557..820)
                     /locus_tag="PLN77_10625"
     CDS             complement(557..820)
                     /locus_tag="PLN77_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44757.1"
                     /translation="MIDEINSTELHTCLLEITLLPLAHYWHFWYGPLDAAIYGSFILG
                     AKILWHIVGFGQSMRTAAEVNEQLRRRRMETVEERREKMRKRH"
     gene            complement(883..1749)
                     /locus_tag="PLN77_10630"
     CDS             complement(883..1749)
                     /locus_tag="PLN77_10630"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRM44758.1"
                     /translation="MERFRTALQEKNTILIGAGAGLSTSAGFTYSGERFRKYFADFEQ
                     KYGFHDMYSGGFYPYDTLEEYWAYWSRYIYINRYKDAPKPVYQKLLSLMKNKSYFVIT
                     TNVDHCFQKAGFDKNRLFYTQGDYGLFQCSEPCCKEKYDNEEIIRKMLLAQGYQILED
                     GSLVPPENGNLSMRIPSELVPYCPHCGKPMSMNLRADDTFVEDKGWHEASSRRIDFLL
                     RHNRGHILYLELGVGYNTPGIIKYPFFSMTAEDKDNLYVCINTSDMRVPDLIRDRAVC
                     IKGDIGEVLDRI"
BASE COUNT          460 a          455 c          401 g          497 t
ORIGIN      
        1 cgcttgacaa agttcgaatt ctatactatg gttatcttac ccggtagtta ctaccgattc
       61 aattctgtta tgaactttgt tgggaggtat gcatgatgta ttccatgaaa gaagcctgca
      121 tccagacagg catgtcgtat gaaaccctga aattttactg caaagaaggc ctcgtcccga
      181 acgtaaaacg cgacagccac aattaccgcg ttttcgacga ccgcgacatc aaatggatcc
      241 agagcctcgg ctgcctgaag cgctgcggca tgacgctggc ggaaatgaaa gaatatctcg
      301 cctactgcct cgaaggtcca tcctccattc cggagcgtaa agaaattctc gcggtaaaaa
      361 aagaagcgct gctcgcatcg atggaaaaac tgcagaaagc catcgattat atcgactgga
      421 aacagaattt ttacgatgat gtcctgtcgg gaaaaatcga atatcacagc aatctgattt
      481 ccgatgaaga gattgcacag aaggcacagt agtccttcct atcctatgca aaaaacggcg
      541 ccgatctttt tacagatcag tgccgttttc tcattttttc tcttctttcc tccaccgtct
      601 ccatgcgtcg tctgcggagc tgttcattta cttcggctgc ggtccgcata ctctgtccga
      661 aaccgacgat gtgccacagg atttttgctc ccaggataaa ggatccgtag atggctgcat
      721 ccagcgggcc gtaccagaaa tgccagtaat gtgccagcgg cagcaatgtg atttccagca
      781 ggcaggtatg caactctgtt gaatttatct cgtcaatcat gtcatcatct cgtcacgtaa
      841 gtgacgtgat taattttctc gaatattata tgtttttccg tctcagatcc gatccagcac
      901 ttccccaata tcccctttaa tgcacaccgc tctgtcacgg atcagatccg ggacacgcat
      961 atcgctggtg ttgatgcaga cataaagatt atctttatct tctgctgtca tgctgaaaaa
     1021 tggatatttg atgattcccg gcgtgttgta tcccacgccg agttccagat acaggatatg
     1081 gcctctgttg tgacgaagca ggaaatcgat gcgtcggctg gatgcctcat gccagccttt
     1141 atcttccaca aacgtgtcat ccgcgcgcag attcatgctc atcggttttc cgcagtgtgg
     1201 gcagtacggc accagttcgg atgggatccg catgctcagg tttccgttct ccggcggaac
     1261 aaggcttccg tcttccagga tctgataccc ctgggcaagc agcatctttc gaatgatttc
     1321 ttcattgtca tacttttcct tgcagcacgg ctcagagcac tgaaacaggc cgtaatctcc
     1381 ctgcgtgtaa aacaaacggt ttttatcaaa tccggctttc tggaagcagt ggtctacatt
     1441 tgtggtgatg acaaaatagc tcttattttt catcaggctg agcagtttct ggtaaaccgg
     1501 ttttggcgcg tccttataac ggttgatgta aatgtatctg ctccagtacg cccagtattc
     1561 ttccagcgta tcgtaagggt agaagccgcc agaatacata tcgtggaaac cgtatttctg
     1621 ttcaaaatct gcaaaatatt tccggaaccg ctcaccgctg taggtaaatc cggccgatgt
     1681 cgaaagccca gccccggctc ctattaaaat cgtgtttttt tcctgcagtg cggtgcgaaa
     1741 acgctccatc tttttttcaa actataaatg agcaaatcca aaatattgca caaaagaaag
     1801 acaccctctg tag
//
LOCUS       DAOMUF010000285         1811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_18241_length_1811_cov_3.146925, whole genome shotgun sequence.
ACCESSION   DAOMUF010000285 DAOMUF010000000
VERSION     DAOMUF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18241_length_1811_cov_3.146925"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PLN77_10635"
     CDS             <1..776
                     /locus_tag="PLN77_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRM44759.1"
                     /translation="CASTMSLMAAGVPIKKPVAGISCGLVTGETDDDYLVLTDIQGLE
                     DFFGDMDFKVAGTHDGITAIQMDIKIHGLTRQIVEEAIARTKQAREYILTEVMEKAIA
                     EPRKTVGEFAPKIIQMMIDPQKIGEVVGQRGKTINAIIDETGVKIDITDDGAVSICGT
                     EQAMMDQAKKYIEIIASDFTEGQILTGKVVSIKDFGAFLEFAPGKEGLVHISKLAKQR
                     VEKVEDVVSLGDVVKVVCMGKDKMGRVSFSIKDVPADAK"
     gene            877..1371
                     /gene="nrdG"
                     /locus_tag="PLN77_10640"
     CDS             877..1371
                     /gene="nrdG"
                     /locus_tag="PLN77_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRM44760.1"
                     /translation="MRYHNITKDDMLNGDGLRVVLWVAGCGHHCKGCQNPITWDPNGG
                     IPFDDAAKAEIFEQLDKDYISGITFSGGDPLYEANRADVTALAKEIKERYPKKDIWVY
                     TGYEWESVCDLELMDYTDVVVDGRFILAQRDVTLEWKGSPNQRVIDVKKTRARGEVVL
                     FGEK"
     gene            1399..>1811
                     /locus_tag="PLN77_10645"
     CDS             1399..>1811
                     /locus_tag="PLN77_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HRM44761.1"
                     /translation="METIKIQYLNDEIERLRYIDGKSDWIDLRAAEEVELKAGEFKLI
                     HLGVAMQLPEGYEAHIVPRSSTFKNFGIIQTNHCGIIDESYCGPNDWFYMPAYALRDT
                     VIHVNDRICQFRIEKHQPTIGFEEVDHLNGEDRGG"
BASE COUNT          518 a          414 c          511 g          368 t
ORIGIN      
        1 tctgtgcatc cacaatgtct ctgatggcag ccggtgtacc gatcaaaaaa ccggttgcag
       61 gtatctcctg cggtctggta acaggcgaga ccgatgatga ttacctcgta ctgaccgata
      121 tccagggtct cgaagacttc ttcggcgaca tggactttaa ggtagctggt acccacgacg
      181 gaatcactgc aatccagatg gatatcaaga tccatggact gacccgtcag atcgtagaag
      241 aggcgattgc cagaacgaaa caggcaagag agtacatcct gaccgaggtt atggagaaag
      301 cgatcgcaga gccgcgtaag acggtcggcg agttcgctcc gaagatcatc cagatgatga
      361 tcgacccgca gaagatcggt gaagttgtcg gccagagagg aaagaccatc aacgccatca
      421 tcgatgagac cggcgtaaag atcgacatca cagacgacgg tgcagtttcc atctgcggaa
      481 ccgagcaggc tatgatggat caggcaaaga aatatatcga gatcatcgca agcgacttta
      541 ccgagggaca gatcctgacc ggaaaagttg tcagcatcaa agacttcggt gctttccttg
      601 agttcgcacc tggaaaagaa ggtctggtac acatctccaa gctcgcaaaa cagcgtgttg
      661 agaaagtaga agacgttgtc agccttggcg atgttgtaaa agtagtctgc atgggcaaag
      721 ataaaatggg ccgtgtaagc ttcagtatca aggatgttcc ggcagacgcg aaataaatag
      781 atctgccaga cgataaagag cggggaacac agaaaaaggg atctgcaaaa cggttcgttt
      841 ttctgtgttt tcttatgtcc aataggagtg aaagctatga gataccataa tataacgaaa
      901 gacgatatgc tgaacggaga cggcctgcgc gtcgtgctct gggtcgccgg atgcggtcat
      961 cactgcaagg gatgtcagaa tccgattacc tgggatccaa atggcggcat cccgtttgat
     1021 gacgcggcaa aggcagaaat ttttgaacag ctggacaagg actacatttc cggcatcacc
     1081 ttctccggcg gagatccgct gtatgaggca aaccgggcgg atgtgacggc tctggcaaaa
     1141 gagatcaagg aaagatatcc gaaaaaagat atctgggttt acaccggata tgagtgggaa
     1201 tccgtctgtg atctggaact gatggattac acggacgtgg ttgtggacgg ccgctttatt
     1261 cttgcccaga gggacgttac cttggaatgg aagggcagcc cgaatcagag agtcattgat
     1321 gtgaaaaaga cgagagcacg cggcgaagtc gtactgttcg gcgaaaagta gtcacgcaaa
     1381 aagaaaagga gacgctttat ggaaaccatt aaaattcagt atttaaacga tgaaattgag
     1441 cgcctgcgct atatcgacgg aaagtccgac tggatcgatc tgcgcgcagc agaggaagtg
     1501 gagttaaagg ccggagaatt caagctgatc cacctcggcg ttgccatgca gctgccggag
     1561 ggctacgagg cgcatattgt gccgagaagc agcaccttca agaacttcgg tattatccag
     1621 acgaaccact gcggtatcat cgacgagagc tactgcggac cgaacgactg gttctacatg
     1681 ccggcgtacg ccctgcgcga caccgtgatt catgtgaatg accgcatctg ccagttccgc
     1741 atcgagaaac accagccgac catcggcttt gaagaagttg accacttaaa cggcgaggac
     1801 cgcggcggaa t
//
LOCUS       DAOMUF010000286         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_18283_length_1808_cov_4.317741, whole genome shotgun sequence.
ACCESSION   DAOMUF010000286 DAOMUF010000000
VERSION     DAOMUF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18283_length_1808_cov_4.317741"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..1099
                     /gene="trmB"
                     /locus_tag="PLN77_10650"
     CDS             449..1099
                     /gene="trmB"
                     /locus_tag="PLN77_10650"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRM44762.1"
                     /translation="MRLRNIRGSKDVIASCPFVVQEPESCRGRWAEIFGNENPIHIEV
                     GMGKGRFLMDMAVLHPEINYVGIEMYDSVLLRAVQKREKLETEIKNLFFIRMDARQLP
                     EVFAEGEVDRIYLNFSDPWPKDRHAKRRLTSHEFLARYEKILKKDGTLEFKTDNKDLF
                     DFSLEEMKNAEGWELTAFTYDLHHDVVLSEGNVMTEYEEKFSSIGNPICKMEARFL"
     gene            1173..1676
                     /locus_tag="PLN77_10655"
     CDS             1173..1676
                     /locus_tag="PLN77_10655"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRM44763.1"
                     /translation="MVTKDADGTWNEDLCTSGYVGYGGVGETTEWNRKTPRGQFSFTY
                     AFGILPNPGTELSYTQVDDTYYWVDDVNSRYYNQFVTTKTTPKAWNSAEHIIEINPAY
                     HYVLSLDYNPNCTPGVGSAIFLHCSTNRPTGGCISVPEDQMITILKNVQPGCQTIIDS
                     ASELKNY"
BASE COUNT          520 a          380 c          422 g          486 t
ORIGIN      
        1 cgatctctgc aagtttgtta attacagatt ccatagcatc gctttccctc cttatttatt
       61 taaaagacaa caaagacgag gaaaactccc cgcatctttg ctgttagtat tctacacctt
      121 tttttccaaa gttgacatat tgaaatcgta aaaaaaagtg ggatttcgat cgacttttta
      181 attaaaaata gacaatgggt catatttttt cttccaatct caatagccaa aagatggtcc
      241 ctcatatgat ttcgatatgg ttccaatccg attgtcatat tgctgataag aaatgctatg
      301 tgcaggttga tattgtatat agtattttga aaacttcgaa aatttgaaaa cgggattttt
      361 ctgttttgct ttttcgggag ctatgatata ctggatatga ttggctttta ttctaatatt
      421 tatcacttaa gaaaggaatt ttacgcatat gcgccttaga aatatcagag gatcaaaaga
      481 tgtgattgct tcctgtccgt tcgttgtgca ggaaccggag agctgccggg gacggtgggc
      541 tgaaattttt ggaaatgaaa atccgattca tattgaggtc ggtatgggaa agggccgctt
      601 tttgatggat atggcggttc tgcatccgga aatcaactac gtcggcattg aaatgtacga
      661 cagcgtgctg ctccgcgcag tacagaaacg ggaaaaactg gagacggaaa tcaaaaacct
      721 gttctttatc cgcatggatg cacgccagct tccggaagtg tttgctgagg gcgaggtaga
      781 ccgcatttac ctcaacttct ccgatccgtg gccgaaagac cgccatgcga aacggagact
      841 gacgtcgcat gagtttcttg cacggtacga gaagatttta aagaaggacg gaacactgga
      901 attcaaaacc gataataaag atctgtttga cttctctctt gaggagatga aaaatgcaga
      961 gggatgggaa ctcactgcat tcacatacga tctgcaccac gatgtggttc tctcagaagg
     1021 aaatgtaatg acggaatatg aggagaagtt ttcctcaatc ggaaacccga tctgtaagat
     1081 ggaggcacgg tttctgtaaa aaacatttct gcatgtaagg gggatttgaa aacgaaaaga
     1141 aaagttctgg cggcaccagt gcgacagtgg ccatggtaac gaaagatgcg gacggcacct
     1201 ggaacgagga tctttgtaca agcggctatg tcggatacgg cggtgtaggc gaaactaccg
     1261 aatggaaccg caaaacaccg cgcggacagt tcagctttac gtatgccttc ggcattctcc
     1321 cgaatcccgg aacggaactt tcctataccc aggtggatga tacgtattac tgggtcgatg
     1381 atgtaaattc cagatactac aatcagttcg tcacgacgaa gacaacgccg aaagcctgga
     1441 attccgcgga gcacatcatc gagatcaatc cggcctatca ttacgttctg tccctcgatt
     1501 acaatccaaa ctgcactccg ggtgtcggtt ctgccatctt cctgcactgc agcacaaacc
     1561 ggccgactgg cggatgcatt tccgttccgg aagaccagat gattacgatt ctgaaaaatg
     1621 ttcagccggg ctgccagact attattgatt cggcgagcga gctgaagaac tattgacatt
     1681 ttttacttgg aatgcaaaac cgacagtgaa aagatctatt tttttaagat ctctccgctg
     1741 tcgttttttt gtgcgtaaaa caagaaaaac accgtccccg cagctctcag gtctgcaaaa
     1801 acagtgct
//
LOCUS       DAOMUF010000287         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_18463_length_1794_cov_3.653824, whole genome shotgun sequence.
ACCESSION   DAOMUF010000287 DAOMUF010000000
VERSION     DAOMUF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18463_length_1794_cov_3.653824"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..495)
                     /locus_tag="PLN77_10660"
     CDS             complement(4..495)
                     /locus_tag="PLN77_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HRM44764.1"
                     /translation="MEAFTGKVWVLGDDIDTDIIIPTEYLALKTIDDMKQYGFSPLRP
                     ELAGQIEKGDIIVAGKNFGCGSSREQAPEIIKALGISCVIAKSFARIFFRNSINNGLL
                     LIEQPDLHDDIKEGDTITVKMNEHVDYNGKTYPIAALPENLMDIIQAGGLVKAMRKRN
                     GLD"
     gene            complement(541..1791)
                     /locus_tag="PLN77_10665"
     CDS             complement(541..1791)
                     /locus_tag="PLN77_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRM44765.1"
                     /translation="MGMTIAEKIIAAAAGVPEVKPGDIHTVTLDRLMSNDGTTHLTVD
                     MYRNKLNHPHIADPKKLVFIVDHNVPSDSPKTAASQKKMRDFARENNIDFWEGKGVCH
                     QVMIENYVRPGELIFGADSHTCSYGALGAFGTGVGCTDFLYGMVTGTSWVLVPESVKF
                     NLVGKLPEGVYARDLILTIIGEVGANGCNYQVMEFTGEGAKTLSISDRMALCNMAVEA
                     GAKTGIFEADEKAIEYLKEHGREPKEIFHSDPDAKYVREYTFDLSKVRPVVAKPDFVD
                     RVVPAEETCGTPINEAFLGSCNNGRIEDLRVGAEIIRGKKVAEGVRFLVVPASQTIYR
                     QALKEGIIDTFMEAGAIVMNPNCSVCWGSCQGVIGENEVLISTGTRNFKGRAGHPSSK
                     VYLGSAATVTASAIAGKIATADQI"
BASE COUNT          363 a          481 c          457 g          493 t
ORIGIN      
        1 ctcttagtcc agtccgttgc gcttgcgcat tgcctttacc aggccgcctg cctgaatgat
       61 atccatcagg ttctccggca gagctgcgat cgggtatgtt tttccgttgt aatcaacatg
      121 ctcattcatc ttgacggtga tggtgtcgcc ctctttgata tcgtcgtgca gatccggctg
      181 ctcgatcaga agaagaccgt tgttgataga gttgcggaaa aagatccggg caaaggactt
      241 cgcgatgacg caggaaatgc cgagcgcctt gatgatctcc ggtgcctgct ctctcgagga
      301 gccgcagccg aagttctttc ctgccacgat gatgtcgccc ttttcaatct gtccggccag
      361 ttccgggcgg agcggggaaa agccgtactg tttcatatcg tcaattgtct ttaaagcaag
      421 atactcggtc gggataatga tatcggtatc aatatcgtct ccaagcaccc atacttttcc
      481 tgtaaatgct tccatatgtt tattctccca aaaatattat atatccctca aagaaaatgg
      541 tcagatctga tctgccgtcg caatctttcc ggcaattgca gatgcggtta cggttgcggc
      601 agagcccaag tatactttgg agcttggatg accggcacgg cccttgaagt tacgggttcc
      661 ggtgctgatc agcacttcgt tctcgccgat aacgccctga cagcttcccc agcagacgct
      721 acagttcggg ttcatgacga tggctccggc ctccatgaag gtgtcgatga tgccttcttt
      781 taatgcctgg cggtagatcg tctggcttgc cggaacaacg aggaaacgga cgccctctgc
      841 gactttcttt ccgcggatga tctctgcgcc gacgcggaga tcttcgattc ttccgttgtt
      901 gcaggatcca aggaatgctt cattgatcgg cgttccacag gtctcctctg ccggaacaac
      961 gcggtctacg aaatccggtt ttgcaacaac cgggcggact ttggaaaggt caaaggtgta
     1021 ctctctgacg tattttgcgt ccggatcgct gtgaaagatt tcttttggct ctcttccgtg
     1081 ctctttcagg tactcgatgg ctttctcatc tgcctcaaag ataccggtct tggcgccggc
     1141 ctcaactgcc atgttgcaaa gtgccatacg gtcgctgatg cttaaagtct ttgcaccctc
     1201 accggtaaac tccatcacct ggtagttgca tccgtttgcg ccgacctcgc cgatgatggt
     1261 gaggatcaga tctctcgcat aaacgccctc cggcagtttt cctacaagat tgaattttac
     1321 gctttccggt acgagtaccc aggaagttcc cgttaccatg ccgtacagga aatcggtaca
     1381 gccgacaccg gtgccgaatg cgccgagtgc tccgtaagag caggtatggc tgtcagcgcc
     1441 gaagatcagc tcgcccgggc ggacatagtt ctcaatcatc acctgatggc atacaccttt
     1501 tccttcccag aagtcaatgt tgttctctct tgcaaaatca cgcatctttt tctgggatgc
     1561 tgctgttttc gggctgtcgg acggcacgtt gtggtcgacg atgaaaacga gctttttcgg
     1621 atccgcgatg tgcgggtggt ttaatttgtt gcggtacata tcaaccgtca gatgggtggt
     1681 tccgtcgttg ctcatcagac gatccagtgt tacggtgtgg atatctcccg gctttacttc
     1741 cggaacgccg gctgcagcgg cgataatttt ctctgcaatt gtcattccca tggt
//
LOCUS       DAOMUF010000288         1786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_18571_length_1786_cov_3.340843, whole genome shotgun sequence.
ACCESSION   DAOMUF010000288 DAOMUF010000000
VERSION     DAOMUF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18571_length_1786_cov_3.340843"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..>1786
                     /locus_tag="PLN77_10670"
     CDS             67..>1786
                     /locus_tag="PLN77_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRM44766.1"
                     /translation="MRFQTENGVLIAQANGETLRIEPWGKDSLRVRATMLPEFSGKDW
                     ALTEVPEKTEAKAEQFEVDHLEIDGSMGKRTSASITNGKIRAVVNFAGVITFYKEEQE
                     ILREYHRCYDGTISKESRCLKTVNREWKGVIGGSEYSLNLKFESNDEEKIFGMGQYQQ
                     KYMDLKGCTLELAQRNSQISVPFAVSSLGYGMLWNNPAVGQVTFGKNYTEWTARSTKD
                     MDYWLTAADTPKEILENYTAVTGRAPKFPEDRMGLWQCKLRYRTQEEVLEVARRYQKE
                     GIKIDQIVIDFFHWTVQGDWKFDEKYWPDPKAMVDELHSMGIKVIVSVWPSVDRKSEN
                     FWPMLDRGLLIKTERGALQTYDFQGDCVEIDVFNPETRKYVWEVCKKNYYDLGIDAFW
                     LDNSEPDFGVYDFDHYRYIDGPALSCSNIYPQLYSRVFYDGMKAEGEENIVNLLRCAW
                     AGSQKYGNVVWSGDVPSTFEAFYDQLQAGLNMGLAGIPWWTTDIGGFMTDDVNDPDFQ
                     QLLIRWYEFAVYSAVFRMHGDRGPHNIPPLDDRDFGGGYLYTGQSNELWSYGEENYRI
                     MKKYYDI"
BASE COUNT          507 a          415 c          485 g          379 t
ORIGIN      
        1 tctgctgaca tgtcagactc tgtgaaatta ccgactgtgg attgaaacaa aaaggaggaa
       61 aaaacaatgc gttttcagac agaaaatggt gtgttaattg cacaggcaaa cggagaaaca
      121 ctgcggattg agccgtgggg aaaagattcc ttacgtgtcc gcgcaaccat gcttccggaa
      181 ttttccggaa aagactgggc gctgaccgaa gtgccggaaa aaacagaggc aaaagcagag
      241 cagtttgagg tcgatcatct ggaaatcgac ggatcgatgg gaaaaagaac cagcgcatcg
      301 atcaccaatg gaaaaatccg tgctgtggtc aactttgcag gagttatcac cttctacaaa
      361 gaagaacagg aaattttaag agaatatcac cgttgctacg acggaacgat cagcaaagag
      421 agccgctgct taaaaacggt caaccgtgag tggaaaggtg tgatcggcgg aagcgaatac
      481 agcctgaacc tgaaatttga gagcaacgac gaagagaaga ttttcggaat gggccagtat
      541 cagcagaaat acatggatct gaagggatgc accctggagc tggcgcaacg gaactcccag
      601 atcagcgtac cattcgccgt atccagctta ggatatggta tgctgtggaa caatccggct
      661 gtcggacagg taaccttcgg aaagaactac accgaatgga ctgcacgctc gaccaaagat
      721 atggactact ggctgaccgc agccgataca ccgaaagaga tcctggaaaa ttacaccgca
      781 gtgaccggcc gcgcgccgaa attcccggag gaccgtatgg gcctgtggca gtgcaagctg
      841 cgctaccgta cgcaggagga agtgcttgaa gtggcacgcc gctaccagaa ggaaggcatc
      901 aaaatcgatc agattgtcat cgatttcttc cactggaccg tacagggcga ctggaaattc
      961 gatgaaaaat actggccgga tccaaaagcc atggtggacg aactgcattc catgggaatc
     1021 aaagttatcg tttccgtatg gccaagcgta gaccggaaga gcgagaattt ctggccgatg
     1081 ctggaccgcg gcctgctgat taaaacagag cgcggcgccc tgcagacgta tgatttccag
     1141 ggggactgcg tggaaatcga tgtgttcaac ccggaaacca gaaaatacgt ctgggaagtc
     1201 tgcaagaaaa actattatga tttgggaatt gatgccttct ggctggacaa ctcagaaccg
     1261 gatttcggcg tttacgattt cgatcattac cgctatatcg acggacctgc tctttcctgc
     1321 agcaatatct atccgcagct gtacagccgt gtcttctatg acggcatgaa agcagaagga
     1381 gaagaaaata ttgtaaacct gcttcgctgc gcctgggccg gaagtcagaa atacggaaat
     1441 gttgtatggt ccggcgatgt gccgagtacc tttgaagcct tctatgatca gctccaggcc
     1501 ggccttaata tgggtcttgc cggcattccg tggtggacaa ccgatatcgg cggctttatg
     1561 acagatgatg tcaacgaccc ggatttccag cagcttctga tccgctggta tgagtttgcc
     1621 gtttactctg ctgttttccg tatgcacggc gaccgcggac cgcacaacat tccaccgctc
     1681 gatgaccgtg actttggcgg cggctatctg tataccggcc agtccaatga attgtggagc
     1741 tatggagaag aaaattaccg gatcatgaag aaatattacg acatcc
//
LOCUS       DAOMUF010000289         1752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_19057_length_1752_cov_2.974072, whole genome shotgun sequence.
ACCESSION   DAOMUF010000289 DAOMUF010000000
VERSION     DAOMUF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19057_length_1752_cov_2.974072"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..896)
                     /locus_tag="PLN77_10675"
     CDS             complement(96..896)
                     /locus_tag="PLN77_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRM44767.1"
                     /translation="MLRDITLGQYYPADSILHRLDPRVKFVGTIVYVVSLFLFHNWGY
                     LLGTVFLAVMIKLSKVPFKFITKGLKSIFVLLAITMVFNILFTPGEVLVRIWKITITR
                     EGVAMACRMSVRLIFLMIGSSLMTLTTTPNQLTDALESLLGPLKKIHVPVHEISMMMS
                     IALRFIPILLEETDKIMKAQMARGADFESGNIIQRVKNMVPLLVPLFISAFRRANDLA
                     LAMEARCYHGGEGRTQMKPLRYQTRDYAAYGVLMAYLVCSIGLRVCGL"
     gene            complement(890..>1752)
                     /locus_tag="PLN77_10680"
     CDS             complement(890..>1752)
                     /locus_tag="PLN77_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRM44768.1"
                     /translation="TYTYSPGTAYEMHALEHINLEFPNGQFVGIIGHTGSGKSTLVQH
                     LNGLIRPTAGRVLYNGEDIWKEGYPLRELRSHVGLVFQYPEHQLFETDVITDVCFGPK
                     NQGLSSEECRKRAEEALDHVGFPKEMYEKSPFELSGGQKRRVAIAGVLAMNPDFLVLD
                     EPTAGLDPKGRDEILDQLALLHKTRGISVVLVSHSMEDIAKYVERIIVMNHGHVAFDD
                     TPKKVFAHYKELEKIGLAAPQMTYIMHALKEHGMDVDVTATTIEEARDTILAALESQK
                     KNGKKGAEVC"
BASE COUNT          427 a          495 c          377 g          453 t
ORIGIN      
        1 ccgtttggct gcacttgcca tccacagtaa tttgtgccgt catacgctac aatcagctta
       61 attctgcgca ttcttctcct ccgttttcgt caaagtcaga gtccgcacac gcgaaggccg
      121 atactgcaca caaggtatgc cataagaacg ccatacgctg cataatcgcg cgtctgatac
      181 cgaagcggtt tcatctgtgt tcttccctct cctccatggt agcagcgtgc ttccatcgcc
      241 agcgccagat catttgcacg gcggaaggct gaaataaaca gcggcaccag aagcggcacc
      301 atgttcttta ctctctggat aatatttccg ctctcaaaat ccgcgccgcg cgccatctgc
      361 gctttcataa tcttgtctgt ctcttccaga agaatcggga taaaacggag cgcgattgac
      421 atcatcatgg aaatctcatg caccggcaca tggatttttt tcaatggtcc gagcaggctt
      481 tcgagtgcat ccgtcagctg gttcggtgtt gtcgtcagcg tcatcagtga agagccgatc
      541 atcaggaaaa tcagacgcac cgacatacgg catgccatag ccacaccctc acgggtgatc
      601 gtaattttcc agatacggac gagcacctca cccggcgtaa ataagatgtt aaataccatc
      661 gtaatcgcaa gcagcacaaa aattgatttc agacccttcg tgataaattt gaacggtacc
      721 ttcgacagtt taatcatcac cgcaaggaat acggttccaa gcagatagcc ccagttgtgg
      781 aacaaaaaga gggaaacgac ataaacgatc gttcctacaa atttcacacg cgggtcaagc
      841 cggtgcagaa tggaatctgc cgggtaatac tgtcccagag taatatctct taacatactt
      901 ctgctccttt tttcccgttt ttcttctgac tttccagtgc cgcaagaatc gtatcgcgtg
      961 cctcctcaat cgtcgtcgcc gttacgtcca cgtccatgcc gtgttccttt agcgcatgca
     1021 taatatacgt catctgcggc gccgcaagac cgattttctc gagctcttta tagtgagcga
     1081 agactttctt cggtgtatcg tcaaacgcca catgaccgtg gttcatgaca ataatccgct
     1141 ctacatattt tgcaatatcc tccatgctgt gcgatacgag cacgaccgaa atgccgcgcg
     1201 tcttatgcag caatgcaagc tgatcgagga tttcatcgcg tcccttcgga tcaaggcctg
     1261 cagtcggctc gtcaagcacc agaaaatccg ggttcatggc aagcacaccg gcaatcgcaa
     1321 cacgacgttt ctgacctccg gagagttcaa acggcgattt ttcatacatt tcctttggaa
     1381 atccgacatg atccaacgcc tcttccgccc gttttctgca ttcctccgat gaaagtccct
     1441 gatttttcgg tccgaagcag acatctgtga tcacatccgt ctcaaaaagc tgatgctccg
     1501 ggtactggaa aaccagtccc acatggcttc gaagctctct tagcggatat ccttctttcc
     1561 agatatcctc tccgttatac agcacccgtc cggcagtcgg tcggatcaga ccgtttaaat
     1621 gctgcacaag cgtcgatttt ccgcttcctg tatgaccgat aatccccaca aactgaccgt
     1681 tcggaaattc aagattaata tgctccaggg cgtgcatttc atatgccgtt cccgggctgt
     1741 atgtgtacgt ta
//
LOCUS       DAOMUF010000290         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_19287_length_1735_cov_2.283929, whole genome shotgun sequence.
ACCESSION   DAOMUF010000290 DAOMUF010000000
VERSION     DAOMUF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19287_length_1735_cov_2.283929"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="PLN77_10685"
     CDS             <1..126
                     /locus_tag="PLN77_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRM44769.1"
                     /translation="LYNSLYGPITPNVPASILQLHPDLTVVADEDALSMIREKIK"
     assembly_gap    314..343
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<344..614)
                     /locus_tag="PLN77_10690"
     CDS             complement(<344..614)
                     /locus_tag="PLN77_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRM44770.1"
                     /translation="MNILFFLIPKSEVAYIFEDETLRQTLEKMEHRKYSCIPILTMDG
                     KYLGTISEGDLLWGMRRADIHDIKETEGIPIMAIPRRATYKPVHAD"
     gene            complement(663..1673)
                     /gene="asnA"
                     /locus_tag="PLN77_10695"
     CDS             complement(663..1673)
                     /gene="asnA"
                     /locus_tag="PLN77_10695"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRM44771.1"
                     /translation="MNTITIPATYHADLNLHDTQIAIKTVKDFFQQTLSQKLNLLRVS
                     APTFVAPESGLNDNLNGVERPVSFDIKAIEGSNAEIVHSLAKWKRYALKKYGFSHGEG
                     LYTDMVAIRRDEDLDNIHSVYVDQWDWEKIISKEERSMETLKETVRTIYSVLRKTEKY
                     MAVQYDYIEEILPKDIFFITTQELLDMYPDCTPREREYRITREKGAVFLMKVGKTLTN
                     GERHDGRAPDYDDWELNGDILVYYPVLDIALELSSMGIRVDEDALDRQLTEAGCDDRR
                     ELPFQKAILNKELPYTIGGGIGQSRICMFFLRKAHIGEVHVSLWPKEITDAAETHGLQ
                     LL"
BASE COUNT          393 a          373 c          440 g          499 t
ORIGIN      
        1 ctgtataatt ccctgtacgg accgatcaca ccgaacgtgc cggcatctat cctgcagctt
       61 cacccggatc tcacggttgt ggcagatgaa gatgcactga gtatgatccg cgaaaaaata
      121 aaataaaaat ccggaataca actgccggat caaaaaagga tggcttccga ttcctacctg
      181 tgcaggaacg gaagccatcc tttttctttc cgctgctcag tggatttctt caccggcagt
      241 tttttgtgcg gcttttattt cgttgtaaca gtatttgatg atagattttc tggttatgat
      301 tccgatgaag gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaatctgc atggaccggt
      361 ttgtaggtgg cacggcgtgg aatcgccatg attgggattc cctctgtttc cttgatgtcg
      421 tggatatctg cacggcgcat gccccagagg agatctccct ctgagatggt gccgagatat
      481 ttgccgtcca tggtgaggat aggaatgcag gagtacttgc ggtgctccat tttttcgagg
      541 gtctggcgaa gagtttcatc ctcaaagatg taggctactt cgcttttggg aatcagaaaa
      601 aacagaatgt tcacgttatt acctcatttc cgcgaaaatt ataaataaat ctgtaggctg
      661 ttttagagaa gctgaagtcc gtgtgtctct gcagcatctg tgatctcctt cggccagaga
      721 gagacatgta cttctccgat gtgtgcttta cgaaggaaga acatacagat cctggactgt
      781 ccgataccac caccgatggt gtacggaagc tctttgttca ggattgcttt ctggaatgga
      841 agctctcttc ggtcatcaca gcctgcttcg gtaagctgac ggtcaagtgc gtcctcgtca
      901 acacggattc ccattgagga aagctcaagg gcaatgtcaa gtaccggata gtatacgagg
      961 atatcaccgt tcagttccca gtcatcatag tccggggcac gtccgtcgtg gcgttcgccg
     1021 ttggtgaggg ttttgccgac tttcataagg aagacagcgc ccttttcacg ggtgatccga
     1081 tattcacgtt ccctcggtgt gcagtccgga tacatgtcaa gaagctcctg agttgtgata
     1141 aagaagatat ccttcggaag gatctcctcg atgtagtcat actgtacagc catgtatttc
     1201 tcagttttac gaaggacact gtaaatggtg cgtacggttt ccttgagagt ctccatggaa
     1261 cgttcttctt tggagatgat cttttcccag tcccactggt ctacgtaaac agagtggata
     1321 ttgtccagat cttcatcgcg gcggattgct accatatctg tgtagaggcc ttcaccgtga
     1381 gaaaaaccgt attttttcag agcgtagcgt ttccatttcg caagggagtg tacgatctcg
     1441 gcgttgctgc cttcgatagc cttgatatca aagcttaccg ggcgttctac accgttaagg
     1501 ttgtcattaa ggccggattc cggtgctaca aatgttggag cggatactcg aagaaggttc
     1561 agcttctggg agagtgtctg ctggaagaaa tcttttactg ttttgatcgc aatctgtgtg
     1621 tcatggaggt tcaggtctgc atgataggtt gcggggattg tgattgtatt cattatcggc
     1681 tcctcctgat gatttttagg cttattgctc tgtggggtat tatagcagaa aatgg
//
LOCUS       DAOMUF010000291         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_20361_length_1664_cov_2.331883, whole genome shotgun sequence.
ACCESSION   DAOMUF010000291 DAOMUF010000000
VERSION     DAOMUF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20361_length_1664_cov_2.331883"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLN77_10700"
     CDS             <1..206
                     /locus_tag="PLN77_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44772.1"
                     /translation="FNARIPLETEEEKRRALHDVICNMKENSIAYQSAHMNVTDMAAL
                     QKLMADKKSLERWKAGELPDFAF"
     gene            312..1411
                     /locus_tag="PLN77_10705"
                     /pseudo
     CDS             312..1411
                     /locus_tag="PLN77_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246158.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            1441..>1664
                     /locus_tag="PLN77_10710"
     CDS             1441..>1664
                     /locus_tag="PLN77_10710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM44773.1"
                     /translation="MQLSKRLSAVAEFVTPGGCLVDVGTDHGYVPIALLEQKKISSAI
                     AMDVNRGPLERAREHIAQYNMGDYIETRLS"
BASE COUNT          469 a          371 c          486 g          338 t
ORIGIN      
        1 tttttaatgc cagaatcccg ctggagacag aggaggaaaa acggcgcgcg ctgcatgatg
       61 tgatctgcaa tatgaaagaa aacagcattg cgtatcagtc ggcacatatg aatgtgaccg
      121 atatggcggc gctgcagaag ctgatggcgg acaaaaagag cctggagcgg tggaaagcag
      181 gggaacttcc ggatttcgct ttttaaggag gcctggatac caaagaaaaa ccctggaatc
      241 ttctgttctg cgtgtttaat ttcgcagggc agggacagaa tatatacaag ctatggaagg
      301 atggacagtc aatggagttc gataaagcaa aatttatgga aaaattaaaa gagctggtag
      361 catacggcaa gaagaaaaag agcgtcctgg aatatcagga aatcaacgac tttttccgcg
      421 atatgccgct ggatgcggag cagatggaga aggtatttga atacctggag gcaagcggta
      481 tcgacgtact gcgtctctcc gatatgaccg aggatccaaa tgcggcagac cgtgatcttc
      541 tccttctgga cgacgatgac atcgaccttt ccgaggaaga agagatcgat atggaaaaca
      601 tcgacctttc cgttccggaa ggcgtcagca ttgaggaccc ggtccgcatg tacttaaaag
      661 agatcggtaa agtgccgctt ctttccgcag aggaagagat caagctggca cagagaatgg
      721 aagagggcga tgaggatgcg aaaaaacgtc tggcagaggc caaccttcgt ctcgttgttt
      781 ccattgcaaa acgctatgtc ggccgcggca tgctgttcct ggatctgatt caggagggaa
      841 atttaggact gatcaaagca gtgcaacgtg gtggatccgc caggcgatca caagagcgat
      901 tgcagaccag gcgagaacga tccgtattcc ggttcatatg gtagagacca tcaacaagct
      961 gatccgcgtt tcccgtcagt tactgcagga gttaggccgc gaacctactc cggaagagat
     1021 cgcagaggag atgcacatgt ctgtagagcg tgtgcgtgag atcctgaaaa tctcccagga
     1081 accggtttcc ctcgagacac caatcggtga ggaagaggac agccacctcg gcgacttcat
     1141 ccaggatgac aatgtgccgg taccggcaga cgcggcagca ttcactatgc tgaaagagca
     1201 gctcgaggat gtgttaagca cgctgactga ccgtgagcag aaggttctgc gtctccgttt
     1261 cggactcgat gacggccgtg cgagaaccct cgaagaggtt ggtaaagagt ttaatgttac
     1321 aagagagcgt atccgtcaga tcgaggcgaa agcgctccgt aaactgcgcc atccgagccg
     1381 ttccagaaag ctgaaagatt acctggacta atctgcgagg aagaaaagga cgacaggaaa
     1441 atgcagttat caaaaagact ttccgcggta gcggagtttg tcaccccggg cggctgcctg
     1501 gtcgatgtgg gaacagatca tggctatgtg ccgattgcgc tgctggaaca gaagaagatt
     1561 tcctccgcaa tcgccatgga tgtgaaccgc ggaccgctgg agcgggccag ggagcatatt
     1621 gcgcagtata acatggggga ctacatagaa acaagacttt ccga
//
LOCUS       DAOMUF010000292         1593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_21538_length_1593_cov_4.164499, whole genome shotgun sequence.
ACCESSION   DAOMUF010000292 DAOMUF010000000
VERSION     DAOMUF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21538_length_1593_cov_4.164499"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLN77_10715"
     CDS             complement(<1..184)
                     /locus_tag="PLN77_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRM44774.1"
                     /translation="MPGAGKSSVGVILAKVLGYEFIDTDLVIQHREKRLLREIIADEG
                     NEGFLAIENEVNATLEA"
     gene            complement(254..1273)
                     /locus_tag="PLN77_10720"
     CDS             complement(254..1273)
                     /locus_tag="PLN77_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRM44775.1"
                     /translation="MKVLTFGEVMLRLKTPEHLKILQADTFEANYGGAEANVAVSLAT
                     LGDDVSYVTKVPGHQVGQAAVNEIRRFGVDTSRVLRGGGRVGIYYFEKGTNIRPTSVV
                     YDRAYSAIAMAKAEEFDWKKLLDGVDLFYFSGITPAISTEIEKALENALALCREKKIQ
                     VVCDLNYRGKMWSTKDAQRVMRRLMAYVDVCIANDEDFESSLGIPAYDGDMSRGIEQI
                     ESYKEGMLEIQKQFPNCKAVASVLRNLYTVEDGDWMGIYLKDGKFYESPVHKVHSFEA
                     VGAGDAFGAGLIHAMLHDFEPQKAIDFAISASVLKLMIQHDANVASETEIEQIMKQGG
                     TNLQR"
BASE COUNT          372 a          460 c          317 g          444 t
ORIGIN      
        1 cggcttccag tgtcgcattg acttcattct caatcgcaag aaagccttca tttccttcat
       61 ccgcaatgat ctcgcgcaga agacgtttct cccgatgctg gatcacaaga tccgtatcaa
      121 taaactcata tcccaacacc tttgcaagaa taacaccaac gctgcttttg ccggctcccg
      181 gcattcctat taagataata ttccgtttat tcaagattta ttcctcctgc tgtctcgtaa
      241 atttttctct atcctatctc tgcagattcg ttccgccctg cttcatgatc tgctcgatct
      301 ctgtttcgct cgccacattg gcatcgtgct ggatcatcag ctttaagaca cttgcggaaa
      361 tcgcaaagtc gatggctttc tgcggctcaa aatcatgaag catcgcatgg atcagtcccg
      421 cgccaaatgc atcaccggct ccgactgcct caaagctgtg tactttatga accgggctct
      481 cgtagaattt tccgtctttc aggtaaattc ccatccagtc gccgtcctcc acggtgtaga
      541 gattgcgcag cacactcgca accgctttgc agttcgggaa ctgcttctgg atttccagca
      601 tgccctcttt gtaagactcg atctgctcaa tgccgcgcga catatctccg tcgtacgccg
      661 gaatgccaag tgatgattca aaatcctcat cgtttgcaat gcagacatcc acatacgcca
      721 tcagacggcg cataacgcgc tgtgcgtcct tcgtggacca catttttccg cggtagttca
      781 gatcacatac cacctggatt tttttctctc tgcagagcgc cagagcgttc tccagcgctt
      841 tttcaatctc tgtgctgatg gcaggcgtga taccggaaaa ataaaagaga tctacgccat
      901 caagcagttt tttccagtca aattcctctg cctttgccat tgcaattgcg ctgtaagcgc
      961 ggtcataaac cacgctcgtc gggcggatat tcgttccctt ttcaaaatag taaataccga
     1021 cgcgtccgcc gccccgcagc acacgggatg tatcgacgcc gaaccggcgg atctcattga
     1081 cggctgcctg tcccacctgg tgccccggca cttttgtcac gtacgaaaca tcgtccccga
     1141 gtgtcgccag ggaaaccgca acattcgcct ccgcgccgcc gtaattggct tcaaatgtat
     1201 ctgcctgtaa aattttcaga tgctccggtg ttttcagacg cagcatcact tctccaaaag
     1261 taagtacctt catactaaaa tcccctccaa aagcaatttg cttttttgtc tcaatacctc
     1321 tttatcttac gctgttttcc ccacggacgc aagaaaaacc tgcggaaacc acccccgttt
     1381 tccatcgcag aaaagcacaa aaaaagcggc tttctgtttc gcttcagaaa gcctcttttc
     1441 gtgtatttca ttattctttt actgctccca gtgtaatacc ggttacaaag tatttctgca
     1501 ggaacggata tacaatcagc ataggaacaa gggcgatgaa tacctttgct gcctccagag
     1561 atttgttgga gttctgtgct aactgctgat act
//
LOCUS       DAOMUF010000293         1587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_21667_length_1587_cov_2.624021, whole genome shotgun sequence.
ACCESSION   DAOMUF010000293 DAOMUF010000000
VERSION     DAOMUF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21667_length_1587_cov_2.624021"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /gene="truA"
                     /locus_tag="PLN77_10725"
     CDS             <1..620
                     /gene="truA"
                     /locus_tag="PLN77_10725"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRM44776.1"
                     /translation="EPVEIHGAGRTDAGVHAAGQVANAKFHTDLSDEELLQTINHYLP
                     EDIEVTSLARMPERFHSRLNAVDKTYEYRIVIDGRKHVFERKYVYFPEAPLDVEKMKE
                     ASKAFIGTYDFRSFCANKRMKKSTVRTITGIDFCEKDGILTISYTGTGFLYHMVRILT
                     GTLVEAGMGKRDIVSMTELLKAKNRESAGFLAPACGLLLKEVRYE"
     gene            726..>1587
                     /locus_tag="PLN77_10730"
     CDS             726..>1587
                     /locus_tag="PLN77_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HRM44777.1"
                     /translation="MFQKKRNTFHGGVHPYEGKELSKDKPIVKAQPGNLLYYAVSQHI
                     GAPAVPIVQKGDHVKAGQKIAEAGGFVSAPVYASVSGTVKGIEKKVSAAGSPVDCIVV
                     ENDGLYEKETFEECPHWEKLDADTIRNKIKEAGIVGMGGAGFPTHVKVSPKDPSAIDH
                     ILVNGAECEPYLTSDYRRMLEDPEKVIGGLKIMLHMFPKAKGIICIEDNKPDCIAKFC
                     ELVLPGDNIEVLELKTKYPQGAERMLIYAATGRKVNSSMLPADAGCIVDNIDTVTAIY
                     QAVRFGEPLMS"
BASE COUNT          471 a          354 c          439 g          323 t
ORIGIN      
        1 gagagccggt ggagatccac ggcgcgggca gaaccgatgc cggcgtccat gcggcaggac
       61 aggtcgcaaa tgcaaagttc catactgatc tatccgatga agaacttctg cagacaatca
      121 accattatct gccggaagac atcgaagtta ccagcctcgc gcggatgccg gagcgcttcc
      181 acagccgtct gaatgcggtg gacaaaacat acgaataccg tatcgtgatc gatggcagaa
      241 agcatgtttt cgagcggaaa tacgtgtatt ttccggaagc accgctcgat gtggaaaaaa
      301 tgaaagaagc atcgaaagcg tttatcggaa cgtatgattt caggagcttc tgtgccaaca
      361 aacgtatgaa aaaatcgacg gtacggacga ttaccggaat tgatttctgc gagaaagacg
      421 ggattcttac catttcctat accggaaccg gttttctgta ccacatggtg cggatcctga
      481 ccggaacgct tgtggaggcc gggatgggaa aacgggatat cgtatcaatg acggagcttc
      541 tcaaagcaaa aaatcgcgaa agtgcgggtt ttcttgcacc ggcatgcgga ctcctgttaa
      601 aagaagtccg atatgaataa ccgaaataat cagggcaatg caccagcctt tttggtataa
      661 aaaccgaaaa aaggctgtga attgataaat gcagcataga aaagagaaag gatgaggtga
      721 aaagcatgtt tcagaagaaa agaaatactt ttcatggcgg cgttcacccg tacgagggaa
      781 aagagctctc caaggacaaa ccgattgtca aagcgcagcc gggcaactta ttgtattacg
      841 cagtgtcaca gcacatcgga gcgcctgctg tgccgatcgt acaaaaaggc gaccatgtaa
      901 aagcgggaca gaaaatcgca gaagcgggag gatttgtttc ggctcctgtt tacgcatcgg
      961 tttccggcac ggtaaaagga attgagaaaa aagtttccgc agccggttcc ccggtagact
     1021 gtatcgttgt ggagaacgac gggctgtatg aaaaagaaac atttgaggag tgcccgcact
     1081 gggaaaaact cgacgcagat acgatccgga acaagatcaa agaggccgga atcgtgggaa
     1141 tgggaggtgc aggcttcccg acacacgtca aagtatcccc gaaagatcca tcagcgattg
     1201 atcacattct cgtcaatgga gcagagtgtg agccgtatct gacgagcgac tatcgcagaa
     1261 tgctcgaaga tccggaaaag gtgatcggag gcttaaaaat catgctgcat atgttcccga
     1321 aagcaaaggg aatcatctgc attgaggaca acaaaccgga ctgtatcgcg aaattttgcg
     1381 agctggtgct tccgggggac aatattgagg ttctggaact taaaacgaaa tacccgcagg
     1441 gtgccgagcg tatgctgatc tatgcagcaa cggggcgaaa ggtaaattcc tccatgctgc
     1501 cggcggacgc gggctgtatc gtggataaca tcgataccgt gaccgctatt taccaggcag
     1561 tccgcttcgg cgaaccgctg atgagcc
//
LOCUS       DAOMUF010000294         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_21906_length_1572_cov_2.025709, whole genome shotgun sequence.
ACCESSION   DAOMUF010000294 DAOMUF010000000
VERSION     DAOMUF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21906_length_1572_cov_2.025709"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLN77_10735"
     CDS             <1..652
                     /locus_tag="PLN77_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRM44778.1"
                     /translation="VGKNARLRYSTIENWSKNMYNLNTKRAQVEEGGKIEWVSGSFGS
                     HVSYLYPMSVLKGRGAKMEFTGITFAGKGQNLDTGAKVVHSAPDTSSYINTKSISKDG
                     GISTFRSSVSATKAAKNTKSAVSCQSLMLDDISRSDTIPAMDIRTKDANIGHEAQIGS
                     ISDEAVFYLMSRGMTEEDARACIVSGFADDVSKELPLEYAVEMNNLIRLEMKGSIG"
     assembly_gap    741..750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <751..>1572
                     /locus_tag="PLN77_10740"
     CDS             <751..>1572
                     /locus_tag="PLN77_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRM44779.1"
                     /translation="AGAPCEICAEELSADDEITGFPEAMNTGAGKETDVLFAPEQTKN
                     WSVSAKEGQTKQVTLSIGQGKETVSSGKIRAVAEEGASLTVFEVFEPAQAAGQLAVRT
                     ELYAKKDSRIRLVQVMMRGEGQELLNDVGCICEENGALDLLQVVVGKGDVYDGIWTEL
                     QKDHASLQAEIGYLLQNQQKFDVNLNVRHFGKVTKSTIQADGTLMDAAEKIFRGTIDF
                     VRGSADSVGAETEQVLLLGDDVVNKTIPVILCAEENVQGSHGAAIGELDEETLFY"
BASE COUNT          433 a          355 c          467 g          307 t
ORIGIN      
        1 tgtcggaaag aatgcaagac tccgttattc tacgattgaa aactggtcga agaatatgta
       61 caacctgaac acgaagcgcg cgcaggtcga ggagggcgga aaaatcgagt gggtttccgg
      121 ttcattcggc tcccacgttt cctacctgta cccgatgagc gtgctgaaag gaagaggcgc
      181 aaagatggaa tttaccggca tcacgttcgc cggtaagggc cagaatctcg ataccggagc
      241 gaaggttgtc cacagcgcgc cggatacgag ttcctatatt aacacgaaat cgatctcgaa
      301 ggacggcggc atcagcacct tccgttcgtc agtctctgca acaaaagcgg caaagaacac
      361 gaaatcagcc gtttcctgcc agtctctgat gctcgatgat atctcccggt cggacacgat
      421 tccggcgatg gatatccgta caaaagatgc gaacatcggc catgaagcac agatcggaag
      481 catcagcgat gaagccgtct tttatctgat gtcgcgcggc atgacggaag aggatgcgag
      541 agcgtgcatc gtcagcggat tcgcggatga tgtttcaaaa gaactgccgc tggagtacgc
      601 ggtggagatg aacaatctga tccgcctcga gatgaagggc agcattggat aaggaggcca
      661 gacacatgga agaacagaac agcatgaaaa tcaaccagct tccatcgaaa acatggtact
      721 ggctgggatt aaatgataca nnnnnnnnnn ggcgggtgcg ccgtgtgaaa tctgcgcgga
      781 agagctgtct gcggatgatg agataacggg ttttccggaa gctatgaata ccggggcagg
      841 aaaagagacg gatgtccttt ttgcaccgga gcagacgaaa aactggagtg tctctgcaaa
      901 ggaggggcag acgaaacagg tcacgctttc catcggacag ggaaaagaga cggtttcatc
      961 cggaaagatc cgcgcggtgg cagaagaggg agcttccctt accgttttcg aggtatttga
     1021 accggcacag gcagcgggac agctcgcggt gcggacagaa ctttacgcga aaaaagacag
     1081 ccgcatccgg ctcgtgcagg tgatgatgcg cggtgaggga caggagcttt taaatgatgt
     1141 cggatgcatc tgcgaggaaa atggtgcact ggatctttta caggtcgttg tcggaaaagg
     1201 cgatgtctat gacggtatct ggaccgaact gcagaaggat cacgcatcac tgcaggcaga
     1261 aatcggctat cttctgcaga atcagcagaa atttgatgtc aatctgaatg tgcgccactt
     1321 cggaaaagta accaagagca cgatccaggc agacggaacg ctgatggatg cggcggaaaa
     1381 gatcttccgc ggcacaatcg actttgtgcg cggttcggca gattccgtcg gagcagagac
     1441 agaacaggta ttgctgctcg gcgatgatgt agtcaacaag acgattcctg tgatcctctg
     1501 tgcagaggaa aacgtacagg gaagccacgg cgcggcaatc ggagaactcg atgaagagac
     1561 actgttttac tt
//
LOCUS       DAOMUF010000295         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Fusicatenibacter saccharivorans isolate AMR_MDS_5737
            NODE_22968_length_1514_cov_2.895134, whole genome shotgun sequence.
ACCESSION   DAOMUF010000295 DAOMUF010000000
VERSION     DAOMUF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867958
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fusicatenibacter saccharivorans (wastewater metagenome)
  ORGANISM  Fusicatenibacter saccharivorans
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Fusicatenibacter.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.138
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:28:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,150
            CDSs (total)                      :: 2,120
            Genes (coding)                    :: 2,088
            CDSs (with protein)               :: 2,088
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 13 of 32
            Pseudo Genes (incomplete)         :: 19 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 3 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Fusicatenibacter saccharivorans"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22968_length_1514_cov_2.895134"
                     /isolate="AMR_MDS_5737"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:1150298"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLN77_10745"
     CDS             <1..559
                     /locus_tag="PLN77_10745"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM44780.1"
                     /translation="TLAANLALALAEEQNRVLLLDCDFRQPALHKIFEIPEKDGKDFG
                     KVVLGKESASGVFEKHKDTNVYTGICRNRLEEPSLAIGGEIFHRILETCRTNMDYIIL
                     DTPPMGMTADAEELAEYADAAVLSVRQDGVLTRDINDAIDALNQKEEKVIGCVFGNVY
                     PGFGERIGNSYGYGYGYGTYSRAER"
     gene            575..>1514
                     /locus_tag="PLN77_10750"
     CDS             575..>1514
                     /locus_tag="PLN77_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM44781.1"
                     /translation="MSRETYETYSAEPEKIDLIAFLAEYFQAFRKFFLGVLILVILMG
                     GGSFLTAKLRYQPMYEAYTSFVVGSNRAVGYSYYDNVTAQQLGKTFPYIVTSGVLKDV
                     VARDLQVGAVTSQIEASVMENTNLFTIRVKDSSPDTAYRVLQSVITNYPEVAEYIIGA
                     TTLTVVDDSGVPVSPINSQDAVHAGMIGAAAGLAVALLLVFIYVRTRKTIRQADDVKK
                     LTNAAFLGNLPEAKIKKRSNVKEQTITICNPKVPDSFKEAMQLIRTRTEDGLGKADCP
                     VLLVTSSVPGEGKTTVAVNLAEAFAKKKYRVVLLDGD"
BASE COUNT          419 a          342 c          427 g          326 t
ORIGIN      
        1 gacccttgcg gcaaatctgg cgcttgccct tgcagaagag cagaaccgtg tactgctttt
       61 ggactgcgat ttccgacagc cggcgttaca taaaatcttt gagattccgg aaaaagacgg
      121 aaaggatttc ggaaaggttg tcctcggaaa agagtcggca tccggcgttt ttgagaaaca
      181 caaggatacc aacgtatata ccggaatctg ccgtaatcgt ctggaagagc cgtcgcttgc
      241 aatcggcggt gagattttcc accggattct ggagacatgc cggaccaata tggactacat
      301 tattctggat acgccgccga tgggaatgac ggcggatgca gaagagcttg cagagtatgc
      361 cgatgcggca gttctttccg tgcggcagga cggcgtactg acgagggata tcaacgatgc
      421 gattgatgca ttgaaccaga aagaggaaaa agtgatcggc tgtgtatttg gaaatgttta
      481 cccgggattc ggtgagcgga tcggcaacag ctacggttat ggatatggat acggcaccta
      541 cagcagggca gaacgataag agggagcaaa gagcatgagc agagaaacgt atgaaaccta
      601 ttcggcggaa ccggagaaaa tcgatctgat cgcctttctg gcagaatatt tccaggcatt
      661 ccgaaaattc tttttgggcg tgctgattct tgtgatcctc atgggaggcg gctcctttct
      721 gacggcaaag ctgcggtatc agcccatgta tgaggcatac acctcatttg tggtcggatc
      781 aaaccgcgcg gtcggctatt cctattatga caatgtgacg gcgcagcagc ttggaaaaac
      841 ctttccttat atcgtaacaa gcggtgtatt aaaggatgtt gtagcacggg atcttcaggt
      901 gggagcagtg acatcccaaa ttgaggcgag cgtgatggaa aacaccaacc tgttcacaat
      961 tcgcgtaaaa gattcttcac cggatacggc atatcgcgta cttcagtccg tcattaccaa
     1021 ttatccggag gtagcagagt acataatcgg tgcgacaacg ctgacggtgg tagacgacag
     1081 cggcgtaccg gtatccccga tcaacagcca ggatgcggta catgccggaa tgatcggagc
     1141 agcggcggga cttgccgttg cactgctttt ggtcttcatc tatgtgagaa caagaaaaac
     1201 aattcgccag gcagatgacg tgaaaaagct gacgaacgca gcgttccttg gaaatctgcc
     1261 ggaggcgaaa atcaagaaac gaagcaacgt aaaagaacag accatcacga tctgtaaccc
     1321 caaagtgccg gattccttca aagaagcaat gcagctcatc cggacaagaa cagaggacgg
     1381 gcttggaaaa gccgactgtc ctgttttgct tgtgacaagc tctgtgccgg gagagggaaa
     1441 gacaaccgtt gccgtgaatc tcgcagaagc attcgcaaag aagaagtacc gcgtagtgct
     1501 gctggacgga gatc
//
